LOCUS       DBMDUH010000001        80306 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_373_length_80306_cov_6.518833, whole genome shotgun sequence.
ACCESSION   DBMDUH010000001 DBMDUH010000000
VERSION     DBMDUH010000001.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 80306)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 80306)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80306
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_373_length_80306_cov_6.518833"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            312..755
                     /locus_tag="ACZPCG_00005"
     CDS             312..755
                     /locus_tag="ACZPCG_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA
                     domain-containing protein"
                     /protein_id="HIR1283488.1"
                     /translation="MTGIIITGHGHFPTGLLSAVSLVAGKPEKVEAVDFTEGMSSEEL
                     KGQLKEAADRLEEQEILILADLTGGTPFNMAAAVKMEETEKHFAVVSGVNMPALVEGV
                     FSRVMYGSLEELAAGVMKAGKEGLCSLEALADEEEEEAPEFEDGL"
     gene            850..1986
                     /locus_tag="ACZPCG_00010"
     CDS             850..1986
                     /locus_tag="ACZPCG_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009254224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HIR1283489.1"
                     /translation="MPFLTKEEVKTAMDRVVKQTELNMEYFGTRFPWPAAKQGTYEII
                     DNIEWTDGFWTGLLWLCYEYTKDEKFRKRAEENMASFLNRVEKRIELDHHDLGFLYIP
                     SCVAGYKLTGSETGKRAGILAADKLMERFQEKGGFIQAWGALGARDNYRLIIDCLLNI
                     PLLYWATETTGDKKYADAAKRHYETACNYVIRDDASAYHTFYFDPETGAPLKGVTRQG
                     YSDDSAWARGQAWGISGIPFNYRYTKDESAFNLFEGMTNYFLNRLPADDVCYWDLIFG
                     DGSGQSRDSSAAAVAVCGIHEMMKYLPETDEEKETYRRAMHGILRSLIQGYVNPDMHP
                     GAPILLHGVYSWHSGKGVDEGNIWGDYFYMEALLRFYKDWQLYW"
     gene            2005..2496
                     /locus_tag="ACZPCG_00015"
     CDS             2005..2496
                     /locus_tag="ACZPCG_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HIR1283490.1"
                     /translation="MAEPNIVAFRIDERLIHGQGQLWIKALGANTVIVADDGASEDPM
                     QQMLMKAVVPKTIAMRFFSVQHTCDVIMKASPKQTIFIVCKTPADALRLVKGGVPVKE
                     INVGNIHRAPGKEEISKYIALGEDDKAALRELRDSYGITFNTRATASGDDGSGKVDLN
                     KYL"
     gene            2518..3306
                     /locus_tag="ACZPCG_00020"
     CDS             2518..3306
                     /locus_tag="ACZPCG_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HIR1283491.1"
                     /translation="MTISITQCVLIGLWTAFCLAGMLFGIYTNRCLIMSAGVGLILGD
                     LPTGLAMGAVGELAFMGFGVSQGGSVPPNPMGPGIVGTIMAITMKDSGIDTGSALALS
                     FPFAVAFQFVITATYTFATTLTGLAYKALDRKDFRGFRLAANATVCIFALVGFIIGFG
                     GAFSAEGLQKVISLIPAWLSTGLGMAGKMLPAIGFAMILNVMAKRELIPFVLLGYIAI
                     AYMKLPVMGVAILGTAIALLVFFNAGRKGEDSVEEVEVEFEDGI"
     gene            3296..4105
                     /locus_tag="ACZPCG_00025"
     CDS             3296..4105
                     /locus_tag="ACZPCG_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HIR1283492.1"
                     /translation="MASNRLEKKDYLKTSLRAFFLQNGFNYGNYQGLGYANVMYPALR
                     KLYKDDDDKLQEALRENIEFFNTNIHFLPFITSLHLVMLENKTPSKEIRNIKMALMGP
                     LAGIGDSLAQFCLAPLFATIGASLAQEGLIMGPILFFLGMNLVLLALKIATGMWGYKV
                     GTSIIESLSTRMEQISSVASMIGVTVISGLAVNFVKITTPIQYVAQMPDNQQKIVAIQ
                     DMIDAIAPNLLPVLFTGLIFYLIKVKKWTTYKLVVLTIIIGVVLSCLHVIA"
     gene            4187..6265
                     /locus_tag="ACZPCG_00030"
     CDS             4187..6265
                     /locus_tag="ACZPCG_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HIR1283493.1"
                     /translation="MTEERKKILAAVTERAENFVKGFDPEFAAGYMKKREEAEAEELL
                     IRAGELMDQTFVFADKWDMEPCREPYTLTEMEWQRTPNGDPEWIFMLNRHDYLHKLMM
                     AYYLTGNEAYTDKLKWYLFHWMCHNPILPEGSDSTRTIDTGIRCMNWEDLILHLAGNG
                     MLTQEELDELLLKLDEQFENLRQRYIGKYTLSNWGLLQTTAICEGYLLFGDSLCHPDT
                     GKWAWQELKRQLDLQVMDDGSHWEQSVMYHMEVLLASMRLMKWKELEENNLCPERYRS
                     DFPEEWDWLKALIEKMSLYVLYCAGPDHRQPAQCDSDRTDVRDIMVKAAVLTGNGVFR
                     FCGYEALDLESLWLFGRKGAERYEAAVSREPERLNYHAQDTGNIYIRSSWKEDANYTY
                     LSCGPQGSGHGHGDLTHISLYYQGKPFLIDSGRYSYREDEPLRMEFKMLQAHNVCVVD
                     DGSVIRPDGSWSFHSYGDCFKTYYREKEGISYCEMAYRGRLASGEGCLVTRKVMTARE
                     GLWLIVNDIWCEGKHTVREYYHLDPGAEARVAEDEILLTNGSVQLKALGEPQEHGPWT
                     QPVPIPFQLESCKVSLEYNRLSDSMCLVKEGSFSDSFVSSSAFCGAGVQVSAPEVYQF
                     GAGEPVSDGRVTAKTFHLSEEESWTFLVWNRETFRGGKMYECSGVPVYGKAGAIHSLG
                     GKQTWIRLRN"
     gene            6351..7355
                     /locus_tag="ACZPCG_00035"
     CDS             6351..7355
                     /locus_tag="ACZPCG_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002571272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR1283494.1"
                     /translation="MKKLTINEIAEKAGVSKTTVSFYLNGKANKMSEETQQRIQQIID
                     ETGYEPSAAARAMKAKSSGLIGVILKDAADPYCARALKGMGEAAGETEYQILIGSSGY
                     TFKNEREHVERMLKAGAEGFIIQATYRFGMLAGELEKKRKPIIYLEAKPYDFKGRYVK
                     GNNYDCVYQVITECIKKGYGDFLMISDGESSMGTAFENTAGYRDALQDAGLEGRTLYV
                     QSGARSAQIFEALKDELDLSLHTLIYVAAPELLKAVFQALRSLPDYERLFPEHLGLIG
                     FDPEGWTRMTSPPVSAIIPPAQEEGKRAMTELLELLDGKKKDTEVVFKNIVKWRGTTV
                     "
     gene            complement(7486..7923)
                     /locus_tag="ACZPCG_00040"
     CDS             complement(7486..7923)
                     /locus_tag="ACZPCG_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002593852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HIR1283495.1"
                     /translation="MLRGIPNCLSPDLLKVLAEMGHGDTLVIGDAFYPSASSARHSML
                     VRADGVRATELLDAILHFFPLDASVKAPVLIMDKQECDKDIPTPIWEEYRQIVRRHDP
                     RGDDCIGMIERFDFYKAAEHAYAVVATGETALYGCIILQKGTL"
     gene            8500..9558
                     /locus_tag="ACZPCG_00045"
     CDS             8500..9558
                     /locus_tag="ACZPCG_00045"
                     /inference="COORDINATES: protein motif:HMM:NF012742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR1283496.1"
                     /translation="MKQNPQKGKRMAVNVREIAKKANVSAATVSRVLHQSEQVKPETR
                     KKVEEAMRQLQVTAGELVRSARMDTRIVGILVPDLSNSFFSECILGIEEYAREAGLTT
                     IICHTKESDQEEVRYLRMLKEMHVCGIIMTPTSDDNDYMNNEYLNLLIDMKIPVVLLD
                     RDVKYSQLDGVFIDNENGAFNAVKLLLENGHRNIAMISGPLNTVPGRGREEGYRNAFE
                     FMNVPLREELIYEGHFSIQGGREATEMILREHPEVTAIFSSNNHMTIGCLSRLFEEGL
                     KIPEDMAVIGFDDIPILNQLGYEITVVGRPTKEMGHQAVKILAKMMNGKRTDMTRRII
                     LIPKLIIRGSERLCRPAD"
     gene            9583..10515
                     /gene="rbsK"
                     /locus_tag="ACZPCG_00050"
     CDS             9583..10515
                     /gene="rbsK"
                     /locus_tag="ACZPCG_00050"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009267117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HIR1283497.1"
                     /translation="MKKVAVIGSFIVDLMARTPHLPEPGETVMGSFFKAGPGGKGGNQ
                     AVAAKRAGSNLVYSTKIGKDSFAEIARASFAENGISMDYVFETEKAATGAALISVDEV
                     TSQNEIIVILGASATYEEEDLEKLKEALSGCEYLLLQMEINREATERLISMAYDMGIR
                     VILNPAPVQQLSEELYSKLYLVTPNEVEARILTEIACDDIEGCRQAARAFMEKGVERV
                     IITIGKRGVYYYDGETEEIIGNYNVKVLDTTGAGDAFNGGLLAGLSQGMELLSAARYG
                     NVVSNLAVTKLGTAPAMPTKQEIDVFIEKEQIKF"
     gene            10531..11565
                     /locus_tag="ACZPCG_00055"
     CDS             10531..11565
                     /locus_tag="ACZPCG_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1283498.1"
                     /translation="MSSITLKNIHKAYGKDVIIPDLNFTIEDGSFTVLVGPSGCGKST
                     TLRMIAGLEKPTSGEIFIDDENVVDVDPGKRGIAMVFQNYALYPTMSVRDNIEFGLKN
                     KKVPKEERNKLIADVSEVVGLTPYLSRKPSELSGGQRQRVALARAMVKNPKVFLMDEP
                     LSNLDAKLRQQMRVELIELHKRLGTTFIYVTHDQVEAMSMGDNIILMDKGIIMQRSTP
                     KEMYHNPQNVYTAQFIGTPPMNIVSNMVKGIQVGFRPEKTSLIDPGDAVLSIQGRIIT
                     KEQLGAEMNYSIETAFGKVMVKTEDDIEGQECRLYIREKNVFAFGEDGNRIPVTEEIK
                     KALEQVEKGV"
     gene            11569..12495
                     /locus_tag="ACZPCG_00060"
     CDS             11569..12495
                     /locus_tag="ACZPCG_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1283499.1"
                     /translation="MEDKKGSVKQKGNFWNHDRKDALKGYLLIFPSILLLGIFTIYPI
                     FYLLDSSLRDGSLLSKKREFVGLENFITLFKSVDFHTTLKNTFIYSVGLVAILMVLAT
                     LLAVWVNGKAQKRLNSVTLAAVFTPHIISLVSVSTVFLWIMDPQIGAINYVLRTLGLK
                     PFPFLASSKTALASLVGMMVWKSLGYYSLLILAALQGVPKDIYEAAEIDDTPKIRTFF
                     RITLPMISPTLFFTTIVATISSFQVFETVNLMTQGGPVNSTNTLVYQIYSDAFKYLKL
                     GPATAEAVVLLTMVGILTFLYFFFLGKKVHYQ"
     gene            12511..13356
                     /locus_tag="ACZPCG_00065"
     CDS             12511..13356
                     /locus_tag="ACZPCG_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1283500.1"
                     /translation="MKKTVMKKKGRILGGALNAALKLLLIFLIAYPFIWMVFTSFKPY
                     KETTIYPPTLLPTNWTTEGYVKALEMVDVATFLKNSLIVSLTVLVLQYLVIVPAAYAF
                     ARCKFRFKGFFFGIVLLGFMIPQQVTFIPIYLMFSKAGLLQSLIPQILPFISNAFGIF
                     LLRQYFMQIPEEIIEAARLDDAGELKIILRIMIPMARPAIFTIGLLSFISSWNSYFWP
                     LIMTTKDAYRTLPIGVAMLNSQEGGRLWNVLMAGNMFLVVPILIVYLFANKQIRKAFA
                     YSGIK"
     gene            13372..14670
                     /locus_tag="ACZPCG_00070"
     CDS             13372..14670
                     /locus_tag="ACZPCG_00070"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013799.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HIR1283501.1"
                     /translation="MKKKLVVLSVDSLFDEDMEFLKTLPNFKKILDQGCYAEGGMRSV
                     YPSFTYPAHASIITGTWPEFHGIYHNEKLDVGNPSPDWYWYHKDLKVDTILDVAHRQG
                     LTTACVGWPCMGADPNVDWLVAEIWPENDKVDPRPILKTGCSENMFEAGGVMERHWHK
                     LRKTTQPFMDQMMVGASCDIIRRYQPDILFIHLAHLDHTRHANGIHGPAVNQAIIAND
                     DWLGRLMEAAMDAGVYEDTNFAVISDHGHLPVKQMFNPNVLLAEEGFIRLDGNGRIAS
                     WDAYCNSTSLSCQVVLKDPADKEVRDKLEALLYQMRKNPAYGVECVYNKEEVEKEQHL
                     TGDFEYVLEGMAGTSFGAACTGEVLVKPDNSDYKFSVASHGHLPHKGPQPVFMIAGPD
                     VKKGAVIPRQRIIDEAPTFAKIFGFDLPDAKGTVLKEILK"
     gene            14705..16063
                     /locus_tag="ACZPCG_00075"
     CDS             14705..16063
                     /locus_tag="ACZPCG_00075"
                     /inference="COORDINATES: protein motif:HMM:NF024808.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1283502.1"
                     /translation="MKRRGALVMSALLMTSLLSGCGGGQADAEKTQEAAQTQAQGENQ
                     SQAEAGETAAVNPDATEVLFWISLSGGMGETIEEIVNNYNASQDKVHVTVEFQGNYYD
                     MAAKLQTAVTSGEMPHVAQLEMGRTKMFADYGVLQDMTELAQEAGLDTSMFFEGLMSS
                     CDWGEGLYALPFNRSTPMFYYNKDMFEEVGLDPEAPPQTWDELKEYAAKLSIPDERWG
                     FEMPIDAWFYEAFIMQSDGRILNEDETDIGFNNETGTEPLAMWKSMISEGIMKAPPGK
                     EYNSYEAARSDFSAGITGMIVTSSGDLGTLNSSCDFEVGTAFLPKNTRFGVPTGGANV
                     VMMAGLEEDSAATMDFLKYLTSPEVAAYWATKTGYVPSSQAAADTDVYQEYISANGNA
                     KTALEQMQYTDIPRPINEDYPQIHSEIMMTEIQRCIEEPSYTPEQAVTAISEQTKALL
                     AK"
     gene            16153..16821
                     /gene="deoC"
                     /locus_tag="ACZPCG_00080"
     CDS             16153..16821
                     /gene="deoC"
                     /locus_tag="ACZPCG_00080"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HIR1283503.1"
                     /translation="MELNKYIDHTYLKPFAMESDIIRICDEAKKYHFASVCVNPCNVP
                     LVAKELAGSDVAVCSVVGFPFGTHTTKTKLFETREALDNGADEIDMVINVGRLIQGDE
                     AYVEQEVAALVQLCHERGKILKVIIETCYLNEEQIKAMCRIVESTHADFIKTSTGYGS
                     RGANFEDIELFKKYLKIPAKMKASGGIRTTEDAKRYVESGCSRLGTSNGVSIMEGTKA
                     QEAY"
     gene            16858..17595
                     /locus_tag="ACZPCG_00085"
     CDS             16858..17595
                     /locus_tag="ACZPCG_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HIR1283504.1"
                     /translation="MKLREREKEEKIEMVRDMIAMLSDVNLFSTVMRLGLALLLGGVL
                     GIERGRRRRPAGLRTYMIVCMASALVMLTGHFLFETFHTGDPSRLGAQVISGIGFLGA
                     GTIIITSKQVKGLTTAAGLWASACLGLAVGAGFYAGAITCGIFLLLVFSCMSKLDTRL
                     RKNAKQISFFAEFKSMKALGEFIHMMRDKNYKIYDVEINRSKDTVGDLVGAVFWVDLN
                     APADHVQVIADFSSFQEVRYVEELEVL"
     gene            complement(18009..19241)
                     /locus_tag="ACZPCG_00090"
     CDS             complement(18009..19241)
                     /locus_tag="ACZPCG_00090"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HIR1283505.1"
                     /translation="MKEKVVLAYSGGLDTTAVIPWLKENFGYDVICCCIDCGQGKELE
                     GLEERAKLSGASKLYIEDLVDDFCDNYIMPCVKADAVYENKYLLGTSMARPAIAKRLV
                     DIARKEGAVAICHGATGKGNDQIRFELSIKALAPDIKIIAPWRMTDVWKMQSREDEIE
                     YCKQHGIDLPFDHSHSYSRDCNIWHISHEGLELEDPSLEPNFANMLVLGVTPEEAPDQ
                     GEYVTMTFEKGVPTSVNGVQMKVSDVIRKLNELGGKHGIGIVDIVENRVVGMKSRGVY
                     ETPGGTILMEAHKQLEELVLDRATMDVKKDMSNRMAQIVYEGKWFTPLREAVQAFVES
                     TQEYVTGEVKFKLYKGNIIKAGTTSPYSLYSESLASFTTGDLYDHHDADGFITLFGLP
                     LKVRAMKMAEMKAEKNSN"
     gene            19490..20530
                     /gene="argC"
                     /locus_tag="ACZPCG_00095"
     CDS             19490..20530
                     /gene="argC"
                     /locus_tag="ACZPCG_00095"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HIR1283506.1"
                     /translation="MIKAGIIGSTGYAGGELARLLLQREDVEIKWYGSRSYIDQKYAS
                     VYQNMFKLVDAECMDDNMKELAEQVDVVFTATPQGLCSTLVNEDVLSKVKIIDLSADF
                     RIKDVEIYKQWYKLEHGSPEYIKEAVYGLPEINRDKIKDARLIANPGCFPTCSFLSTY
                     PLVKEGIIDPNTLIIDAKSGTSGAGRGAKVDSLYCEVNENIKAYGVGVHRHTPEIEEQ
                     LSYAAGKPVTISFTPHLVPMNRGILVTAYASLAKDVTEEEIRAAYEKYYKNEYFVRLL
                     EPGVVPQTRWVEGSNFADVNFKLDPRTKRVVMMGAIDNMVKGAAGQAVQNMNLMFGLP
                     ENTGLKQVPLFP"
     gene            20593..21057
                     /locus_tag="ACZPCG_00100"
     CDS             20593..21057
                     /locus_tag="ACZPCG_00100"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1283507.1"
                     /translation="MSGTMEILIREMTIEDYEAVYHLWTEIRGFGIRSIDDSKEGVER
                     FLKRNPTTSVVAVQNGRIVGNILCGHDGRTGCFYHVCVDPDYRKHGIGYRMVRAAIHA
                     LQKEGVSKISLIAFKSNEIGNAFWQGIGWTEREDINSYEFILNEENITRFVK"
     gene            21071..22297
                     /gene="argJ"
                     /locus_tag="ACZPCG_00105"
     CDS             21071..22297
                     /gene="argJ"
                     /locus_tag="ACZPCG_00105"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - L-glutamate N-acetyltransferase
                     activity, acting on acetyl-L-ornithine as donor [Evidence
                     IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HIR1283508.1"
                     /translation="MYEVIQGGVTAAKGFMAAFTAAGIKYKNRPDMAMIYSQVPCESA
                     GTFTTNLVKAAPVKWDKAQVTSGAKAQAVIVNAGIANACTGKEGMDYCAQTAKAAAEA
                     LNIKEDSVLVASTGVIGMQLPMERIEAGIRAMAPELGDSLESGTEASKAIMTTDTKNK
                     EVAVKVVLGGVPVTIGGMCKGSGMIHPNMCTMLSFVTTDAAISKELLQEALSEDIKDT
                     YNMISVDGDTSTNDTVLLLANGLAGNPEITEKNEDYKAFCEALNYINETLAKKMAGDG
                     EGCTALFEVKIKGAQTKEQAKILAKSVITSSLTKAAIFGHDANWGRILCAMGYSGAEF
                     DPEQVDLYFESAAGSMQIIKDGVAVDYSEEQATKILSEPEVTAIADIKMGTEEAAAWG
                     CDLTFDYVKINADYRS"
     gene            22352..23242
                     /gene="argB"
                     /locus_tag="ACZPCG_00110"
     CDS             22352..23242
                     /gene="argB"
                     /locus_tag="ACZPCG_00110"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HIR1283509.1"
                     /translation="MNLEPMQKAAVLIEALPYIQRFNRKIVVVKYGGSAMVDEELKKK
                     VIQDVVLLKLVGFKPIIVHGGGKEISRWVGKVGMEPYFINGLRVTDEPTMEIAEMVLN
                     KVNKSLVQLVNELGIKAVGISGKDGMMLKCHKKLSGGQDIGYVGEIDSVDPEIIHDLL
                     EKDFLPIICPIGYDEHFQSYNINADDAACAIARAVQAEKLAFLTDVEGVYKDFEDKDS
                     LISEMEVDEAQAFVDSGSLGGGMLPKLQNCIDAIKNGVSRVHILDGRIPHCLLLEIFT
                     DKGIGTAIYNSKEDERYYHG"
     gene            23235..24473
                     /locus_tag="ACZPCG_00115"
     CDS             23235..24473
                     /locus_tag="ACZPCG_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIR1283510.1"
                     /translation="MADEKMINRAEQVLYKTYNRFPVVFDHGKGVTLWDSEGQEYLDF
                     GAGIAVMGLGYADEEYTQAVKAQLDKLTHISNLFYNEPSITAGEKLLKVSGMDKVFFT
                     NSGTEAVEGALKIAKRYHYNKLHETMGDGFDGCEEKEIDMTGEIIAMNHSFHGRSLGA
                     LSVTGNAHYQAPFAPLIPGIRFADFNDLDSVKSQITDKTCAIIMETIQGEGGIYPAEE
                     AFLKGVRALCDEHDILLILDEIQCGMGRTGEMFAWQHYGVKPDVMTVAKALGNGVPIG
                     AFLACGKAATAMVPGDHGTTYGGNPLVTAAAGAVLDIFEKRQITDHVKEVGAYLYEKL
                     EELTKKYDIICGHRGRGLIQGLEFTVPAGPIVSKALLEEKLVLISAGSHIIRFVPPLI
                     IQKSDVDEMAARLERVLKNL"
     gene            complement(24576..24854)
                     /locus_tag="ACZPCG_00120"
                     /pseudo
     CDS             complement(24576..24854)
                     /locus_tag="ACZPCG_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005540551.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
     gene            complement(24855..25127)
                     /locus_tag="ACZPCG_00125"
     CDS             complement(24855..25127)
                     /locus_tag="ACZPCG_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004069026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000987 - cis-regulatory region
                     sequence-specific DNA binding [Evidence IEA];
                     GO_function: GO:0015643 - toxic substance binding
                     [Evidence IEA];
                     GO_process: GO:0044010 - single-species biofilm formation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HIR1283511.1"
                     /translation="MATAPTQIRIDADIKKQATDLFNTLGLDMSGAVNLFLHQCVLRG
                     GLPFNIEMPHYNQRTLDAMDEAKRISRDPDVKGYNSMYELRRTLEE"
     gene            complement(25196..25606)
                     /locus_tag="ACZPCG_00130"
     CDS             complement(25196..25606)
                     /locus_tag="ACZPCG_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1283512.1"
                     /translation="MIRYDRLWDTMTNRGMTQYKLIKFHGFSAGQIGRLKKNMHVSTH
                     TLETLCRLLDCRIEDIVEYRADEQIPSDPPASSPQSPAPDTVPPITDTTPELQDSDFL
                     SVGRSDKPEKTKKGSKAKKASKKSLKNAKKSRKK"
     gene            25756..26631
                     /locus_tag="ACZPCG_00135"
     CDS             25756..26631
                     /locus_tag="ACZPCG_00135"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR1283513.1"
                     /translation="MLDYRIAAVCGAALGAALAARSKYERKSLSVEETEVFSDRLTRD
                     WIFVFLSDLHENEFGPENEQLIAAIDRIKPDGILIGGDLIVTKKRQARTRVSETLLAA
                     LASRYPIYCGNGNHESRMRWRKEEYKDGYEQYRAYLKKLGICLLEDQSVWIGDEIRVS
                     GLELDPCFYKKAYSKKLALMDQAYIEKKLGPASRDHFQILLAHSPLFFEAYRKWGADL
                     TLSGHFHGGTIRLPVLGGVMTPQYQFFLPECAGTHEKGGKYMAVSRGLGTHSINIRLN
                     NRPQLLFVRLKRKRT"
     gene            26634..27434
                     /locus_tag="ACZPCG_00140"
     CDS             26634..27434
                     /locus_tag="ACZPCG_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007709126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="segregation and condensation protein A"
                     /protein_id="HIR1283514.1"
                     /translation="METITYKLEHFEGPLDLLLHLIEKNKIDIYDIPIVEITAQYLAY
                     VRQMEREDLNIVSDFLVMAATLLDIKAKMLLPKEVNEEGEEIDPRAELVSRLLEYKRY
                     KLLAEELAEREEGALMHFYKASTLPPEVAKYQPPVDLDELLDGMTLAKLKRIFEQVMK
                     RQENKVDPIRSKFGTIRREPVSLETKIGSVLAYARKKRRFSFRQLLERQSDKLEIVVT
                     FLAVLELMKIGKIHLTQEEIFGDMDIEALEPEGMEEEPDLAELSYFEE"
     gene            27438..28067
                     /gene="scpB"
                     /locus_tag="ACZPCG_00145"
     CDS             27438..28067
                     /gene="scpB"
                     /locus_tag="ACZPCG_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002578228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HIR1283515.1"
                     /translation="MENSSINVNFEPVENEKDQEAVIEAVLFTIGGAVELRQLAAAIG
                     QDKETAKRAVERLAKRYREETRGMEIKQLEDSYQMCTRSEYYENLIRVAAAPKKQVLS
                     DVVLETLSIIAYRQPVTKMEIEKIRGVKSDHAVNRLVEYDLVYEAGRLDAPGRPALFA
                     TTEEFMRRFGVDSLGELPDVDPEQEALIRAEVEEELQLSVEELQKDMEE"
     gene            28170..28790
                     /locus_tag="ACZPCG_00150"
     CDS             28170..28790
                     /locus_tag="ACZPCG_00150"
                     /inference="COORDINATES: protein motif:HMM:NF014689.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump"
                     /protein_id="HIR1283516.1"
                     /translation="MFQSFILVLALCADTFGAGFAYGTNRIRVGFGKVLFLNGICSVC
                     LGLAMTTGFFAAERLSGINLRTLCGGSLIFMGLFRLTDFSLRRWIRMHWENGRSFCFS
                     HSGIHVLIRIWSDPMEADADGSHSLEWKETFILSLAMSLDSLAAGAAAALSEGRIALT
                     VLLSLLVGTAVTEAGLMAGRFAAEKGQRDLGWLSGLLLIAVGIGKL"
     gene            28912..30318
                     /locus_tag="ACZPCG_00155"
     CDS             28912..30318
                     /locus_tag="ACZPCG_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012739703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HIR1283517.1"
                     /translation="MLQFLNTALKAIDDLVWGVPLIVLILATGIYLTVRLKGMQIRKL
                     PAALRYMVQNEEEGSGEVTSFGALCTAMSATIGTGNIVGVATAIVAGGPGALFWMWIA
                     ALFGMATKYAEGVLAICYRSVDKETGHVLGGPFYYIENGMGKKWRWLGKIFAFFGAGV
                     GLLGIGTFTQVNGITSAVGDFFDSQKACTVALFGREYTVAVLIAGLILTICVALVVLG
                     GLERIATVSQIIVPFMAVFYVAACITILCVNAQRVPMAFKMILESAFGLRPAAGGALG
                     SILLAMQKGIARGIFSNEAGLGSAPIAAAAARTREPVRQGLVSMTGTFIDTIIICTMT
                     GLSIVLTGAYETGLEGVAVTTYAFNHGLPLPAWASSFLLMACLIFFAFTTIIGWNYYG
                     ERCLEYLSGGSRRAVMTYRFLYILAIFIGPFMTVEAVWTIADIFNALMAIPNLIAVLA
                     LSGVVAAETGDYWKRMKK"
     gene            30472..32604
                     /gene="nrdD"
                     /locus_tag="ACZPCG_00160"
     CDS             30472..32604
                     /gene="nrdD"
                     /locus_tag="ACZPCG_00160"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003503530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HIR1283518.1"
                     /translation="MDLTRVKVVKKDGTKEDFNVQKVISAISKSAERAMITFSKAELN
                     FICEFVEEKVQELDQELIPIPQMHNIVEGALEKVNPLVAKSYRDYRNYKQDFVRMLDD
                     VYKKSQSIMYIGDKENSNTDSALVSTKRSLIFNELNKSLYQKFFMTVEELQACREGYI
                     YIHDMSARRDTMNCCLFDVKAVLTGGFEMGNLWYNEPKTLDVAFDVIGDIVLSAASQQ
                     YGGFTVPSVEEILAPYAEKSYEKYIEKYLDLGLAADKAKEVAWKDVQRDMEQGFQGWE
                     YKFNSVSSSRGDYPFITMTAGTGTSRFAKMATMTMLNVRKGGQGKKDHKKPVLFPKIV
                     FLYDEALHGPGGELEDVFEAGIDCSSKTMYPDWLSLTGKGYIASMYKRYGKIISPMGC
                     RAFLSPWYERGGMYPADDKDSPVFVGRFNIGVVSLHLPMILAKARQESRDFYEVLDYY
                     LELIRKIHIRTYAYLGELKASTNPLAYCEGGFYGGHLGLHDKIKPLLKTATASFGITA
                     FNELQQLYNKKSLVEDGQFALEVLEHINKKIEEFKEEDGNLYAIYATPAENLCGLQVK
                     QFREKYGIIENVSDREYVSNGFHCHVTEDITPIEKQDLEYRFWELSNGGKIQYVKYPI
                     SYNKEAIRTLVRRAMKMGFYEGVNLSLAYCDDCGHEELEMDVCPVCGSRNLTKIDRMN
                     GYLSYSRVKGDTRLNEAKMAEIAERKSM"
     gene            complement(32661..32999)
                     /locus_tag="ACZPCG_00165"
     CDS             complement(32661..32999)
                     /locus_tag="ACZPCG_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1283519.1"
                     /translation="MTTMSFELERIEELCRIRGWSHYRLAKEMDVSPNSIGNLFRRTS
                     IPSVPTIRHICEAMGITISQFYSTDGSAATITEQQKAFLELFDRLSPGDKKKAEGYIE
                     GLLAKSQSEP"
     gene            complement(33180..33473)
                     /locus_tag="ACZPCG_00170"
     CDS             complement(33180..33473)
                     /locus_tag="ACZPCG_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002578230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1283520.1"
                     /translation="MRKITTSEQKNIIGSRIRQLRMARKLTQDELAARLQVNGYNFSK
                     LTILRIELGKRLVTDIELKGLCEFFETDPNSLLGYRPDSPKEEALKDPFIRRF"
     gene            33936..34397
                     /gene="mscL"
                     /locus_tag="ACZPCG_00175"
     CDS             33936..34397
                     /gene="mscL"
                     /locus_tag="ACZPCG_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HIR1283521.1"
                     /translation="MKKFIEEFKAFALKGNMVDLAVGMIIGSAFTSIVNSLVNDIFMP
                     VLSIFTGKIDFSNMFIALDGNKYPTLEAAKEVTATINYGSFITQTINFLLMALVVFLA
                     VKAISRLEKIGKQPEAPKAPTEKKCPYCSTMIPLSAVRCPHCTSNLDEQEK"
     gene            34416..34916
                     /gene="nrdG"
                     /locus_tag="ACZPCG_00180"
     CDS             34416..34916
                     /gene="nrdG"
                     /locus_tag="ACZPCG_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HIR1283522.1"
                     /translation="MRYHNITKDDMLNGEGLRTVLWVAGCSHNCPGCHNPVTWDVRGG
                     LPFDEAAKEELFAELEKDYISGVTFSGGDPLHPANREDIARLAEEIRGRYPNKTIWLY
                     TGYDWEQICEFALLKNIDVVVDGEFIEAYKDVSLHWKGSSNQRVIDVQRSLLMGEAVL
                     YEEPDT"
     gene            35000..35515
                     /locus_tag="ACZPCG_00185"
     CDS             35000..35515
                     /locus_tag="ACZPCG_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004840830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase/dUTPase family protein"
                     /protein_id="HIR1283523.1"
                     /translation="MSRIAKFHKVSFEQFKGDWEDTFGKQEDIRSIYEDIRLPERATS
                     GSAGYDFFAPKAFVIQPGETVKIPTGIRAEIDEGWVLALFPRSSLGFKYRLQLNNTVG
                     IIDSDYFYSDNEGHMFIKITNDSNEDKTLELPKGAGVVQGIFLPFGITLDDASSGQRN
                     GGFGSTDKGRN"
     gene            35518..36555
                     /locus_tag="ACZPCG_00190"
     CDS             35518..36555
                     /locus_tag="ACZPCG_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR1283524.1"
                     /translation="MEGSRRRSSYPGGSRPLGAGRPGDAGRRPVRAGSSSGRRNGGNP
                     RRRRRKRLMKRILTAAFCLILTGGIAFGVSRLAGSFHFSKKKKLRAQGIELLRGGDAG
                     GAVSAFDEALAAAGDKSKTFNIDVLSYRAEAEMMLDDFEAAKHSWDLILEQGGDPISG
                     RYMKSFCESRLGNKDQAVALYREALGMEEKGKTSPGYEQALLAAGEACMEAGDHEGAK
                     VLYEEALRTTGGDGRFESRVRCQMGLYQMAEEDYEGAADSFYQGYDRLITSYKAGTGA
                     SLDQAAAAITEENQADWQLLKKLAFYQAAVCEYMQNYEEAKSRFETYIQVFGPDEAAQ
                     HEIDFLNTRIR"
     gene            36564..37826
                     /gene="hflX"
                     /locus_tag="ACZPCG_00195"
     CDS             36564..37826
                     /gene="hflX"
                     /locus_tag="ACZPCG_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HIR1283525.1"
                     /translation="MAELIDLKELEERVILIAVNVGDERETSASLDELEELAQTAGAV
                     PIARIVQNREAVHPGTYLGKGKILEVRELLADTGATGVICDDELSPAQLRNLEDALDT
                     KVMDRTMVILDIFASRAHTREGKIQVELAQLRYRAIRLVGMRNSLSRLGGGIGTRGPG
                     EKKLETDRRLIHQRIGQLKRELEDVKRHREVTRQHREKNYSLTAAIVGYTNAGKSTLL
                     NRLTGAGILAENKLFATLDPTTRAFVMADGQQILLTDTVGFIRKLPHHLIDAFKSTLE
                     EARYSDVILHVVDCSNPQMDMQMHVVKETLRELEIVDKTVVTVFNKIDRLEGKETELL
                     PRDFSSDYQVRISAKTGDGLKDLEQILGGIIRSSRVYLEKVYPYSGAGKIQTIRAYGQ
                     LLSEEYTEQGIAVKAYVPAELFEGLYRD"
     gene            37997..40387
                     /locus_tag="ACZPCG_00200"
     CDS             37997..40387
                     /locus_tag="ACZPCG_00200"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016282789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HIR1283526.1"
                     /translation="MIQVVKRDGETAEFCLKKITEAIKKAFRATGNEFNDEILELLSL
                     RVTADFQPRMKDGSISVEEIQDSVEHVLEQTGYTDVAKAYILYRKQREKIRSMNSALL
                     NYKDVVNSYVKVEDWRVKENSTVTYSVGGLILSNSGAVTANYWLSEIYDQEIADAHRN
                     ADIHIHDLSMLTGYCAGWSLKQLLMEGLGGIPGKITSAPAKHLSVLCNQMVNFLGIMQ
                     NEWAGAQAFSSFDTYLAPFVKADHLSYGEVKKCIESFIYGVNTPSRWGTQAPFSNITL
                     DWTVPDDMAEMNAIVGGREMDFKYKDCKAEMDMVNRAFIETMIEGDANGRGFQYPIPT
                     YSITKDFDWSDTENNRLLFEMTSKYGTPYFSNYINSDMQPSDVRSMCCRLRLDLRELR
                     RKTGGFFGSGESTGSVGVVTINIPRIAYLAEDEADFYRRLDRMMDISARSLKTKREVI
                     TKLLEQGLYPYTKRYLGTFENHFSTIGLIGMNEAGLNTRWIRKDMTQPECQRFTREVL
                     DHMRERLSDYQEEYGDLYNLEATPAESTTYRLAKHDKERYPDIITAGKEGETPYYTNS
                     SHLPVGYTEDIFDALDIQDELQTRYTSGTVFHAFLGEKLPDWKAAASLVRTIAQNYKL
                     PYYTLSPTYSICKSHGYLAGEHFTCPICKQKAEVYSRITGYYRPVQNWNEGKLQEYKD
                     RVTYDTGKSVLKKEPVSMRPVERVEAESQNESGEGLKKKPQLYLFTTRTCPNCRTAKE
                     FLKGVDYQIIDAEEHPELAEKFGIMQAPTLVLVRDGMVQKFANASNIRRFAEEVKA"
     gene            complement(40528..40974)
                     /locus_tag="ACZPCG_00205"
     CDS             complement(40528..40974)
                     /locus_tag="ACZPCG_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HIR1283527.1"
                     /translation="MSSKTKIVVLRMKEIIYTAIFAGLAILLITLCFIMFRHGDENTS
                     ETDAAKAQQTAYVPGVYSTSLTLGNEQVNVEVTVDKDQIRAVTLVPLSEAVATMYPLM
                     QPTMEGLAEQIVNTQSTENLTYPDSSRYTSLALLQAVNTALGKAKP"
     gene            41030..44518
                     /gene="addB"
                     /locus_tag="ACZPCG_00210"
     CDS             41030..44518
                     /gene="addB"
                     /locus_tag="ACZPCG_00210"
                     /EC_number="3.1.-.-"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HIR1283528.1"
                     /translation="MSLQFVMGGAGSGKTRYLYQTAIKQAVEQPDRLFLFVVPEQYTM
                     QTQKELVRLHPRKGLMNMDVLSFKRLAYRVFEDLGVQVPAVLDDMGKSMVLRKVAGMV
                     RRDLGLYGSHLEQPGFISQLKSQISELGQYGIRPEDLKQVEAETDHPLLKDKLKDLGV
                     IYKSFRDYIEDHYITAEEILDILCRELPRWEKLKNSVIYLDGYTGFTPVQYRLAELFM
                     LHAREVVCTVTVDLRENPYEKCGIQHLFYMGRHTVCRLKELADRHHVEIKQEIRCDRH
                     PGWRFKDSESLDFLEQNLYRYTGKVWAGKKPDIRLCQGKTPAEEAAYICTMVNRLVRE
                     DGLRYREIAVITGDPVSYGRELIHPFEEAGIPYFMDQKKSILENPMVELIRAALETVR
                     DCSYESVFRYLKTGLVYDRETGNSEENEDTPVFSRENVSVLTDRLENYVRALGIRGWK
                     KWDQEWTRPSRGGERLNLKELNQYRQWVLAPLKELREAFSQEGASISTVTSAVRSFLE
                     KMELKEKLEEYAEFFEMRHCRGDENLAKEYGQVYERVEELLLRLEGLLGEEKADRKNY
                     IQILEAGFEEIRVGVIPATADQVMIGDLTRSRLESVKVLFFAGLNEGLVPQRKSGGSL
                     LTDTDREIFRSFDMELAPTAREDSCIQKFYLYLMLCKPSRKLILTWAASSGDGKSMRP
                     SSLIGEVKKLFGELREEICFEEGYPIQTIWDGRQRLIGMLREARESQEKKPAFLELYR
                     YFYSEEAYREQVKQLVNAAFFTYEDRGIGRAAARALYGRTLEGSVTRLEQFASCAYAH
                     FLKFGLELMERQEYRLEAVDMGNLFHQSIDLCFQAIQSRKLDWKGITEAERKALVREC
                     VEQVTGQYGNTIMESSARNAYLARRVERITDRTIWALAEQVKKGDFVPAGFEVSFSAI
                     DDLKAMRIRLSEDEKLQLKGRIDRLDLCEDEEHIYVKIIDYKSGSTSFDLAALYYGLQ
                     LQLVVYMDAALEMQERKNPGKEAVPAGIFYYNIKDPVVDKDGEMSPEFIEKQILKQLR
                     MNGLVNSDLDVIHHLDREMVKDSDVIPVVFGKDGGVQESRSSVAKAVQFETLRAFVGR
                     KLKASGKQILNGDTALRPYKQGSRTACDYCPYHAVCGFDTKTAGYGFRQLCSMSPNAV
                     WEAIEREEEENGSELD"
     gene            44499..48353
                     /gene="addA"
                     /locus_tag="ACZPCG_00215"
     CDS             44499..48353
                     /gene="addA"
                     /locus_tag="ACZPCG_00215"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HIR1283529.1"
                     /translation="MAVNWTDEQKQVINSRNSSLLVSAAAGSGKTAVLVERIISMISE
                     GDHPLNIDQLLVMTFTNAAAAEMRERIGAAVEKKLREHPLDEHLWLQAALIPQAQIMT
                     IDSFCLNLIRNHYNSLDIDPSFRIGDEGELTLLRGDVMEELLEACYQEENEDFIRFAE
                     QFGAGKSDKAMEDVIVQAWQFSQSHPWPGEWLDECRRQLKQEAEGNLDESPWMKFLLK
                     DTALQMEELTHQLQAALEVCREEGGPAVYEPMLENDLAGLLGIKKAAERGTFEKLYEA
                     LQAMEFVRLAAARNKAIDEDKKEYVKECRTQVKKLVEKCRESFGFQSPKEAAEAMAGA
                     APVVETLLDMVQKFDDFYRKAKRERNVLDFNDLEHLALEVLLEGDSPRQPSAVAKELQ
                     RQYEEILVDEYQDSNLVQETLIGSISREETGHPNVFMVGDVKQSIYRFRLARPELFME
                     KYASYTREKSPHQMIELRQNFRSRDTVLAFINDIFYQIMTPGLGGIRYTEETALYPGA
                     VFASAEGMAGVPSRLLMADTGSDAYKQLDEDAMGYTAREMEAKMIAGEIRRLTDETDG
                     LLVWDKEQEKYRRVCLGDMVILLRSMTGWSETLVSVLMNEGIPARAQTRTGYFSTTEV
                     ETVLSLLSVIDNPMQDIPLAAVLRSPVVGMSDDEMAWMMASYKKNTKKGQDRGVYGAW
                     KLWEEEREHPGLREALEHPGGAFENETGDSEDAVRIGAAVVPVEQAALIWKKLEDFGA
                     LLKELRTEARYRPIHELLYYVYRRSGYYDYVSAMPAGEIRRGNLDMLVEKAAAYEATS
                     YKGLFHFVRYIERLKKYDTDFGEAAVAGEEKNMVRIMSIHKSKGLEFPVVFLAGMGKK
                     FNQRDAYGTLLLDADMGAATDWLDLKLRTKTPTLKKQVLKRRLELETMGEELRILYVA
                     MTRAKEALIMTGTDRSLSSKLERWRIGRTEGKQLPFTLLSGAGSYLDWILMAAGAVPQ
                     EHFEMIKVQPETLVGEEVTRQMERKMAKEDLLELDAGYTYDEEMKKELEQAFSYAYPY
                     ESDTGLYTLVSVSELKKQSQIGREEEAMGTGREEDGWIRDLDEEKAEDSVKETEAGGT
                     VQEAEASEAGAKEGKKVYGRSGGSKRGTAYHRALECLSFHELHSLSDTKKALFSLYEQ
                     GYLDQESYEWINPWDIWKFVESPLGKSMAKAQAEGRLFKEQQFMIGVPASEMGHEDSE
                     ELVLVQGVIDAFIEEPEGLLLIDYKTDRISGKDKEMGKERLKERYRTQIDCYERALCQ
                     ITGKTVARRCIYSFALREEIEI"
     gene            complement(48375..49205)
                     /gene="thyA"
                     /locus_tag="ACZPCG_00220"
     CDS             complement(48375..49205)
                     /gene="thyA"
                     /locus_tag="ACZPCG_00220"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HIR1283530.1"
                     /translation="MSYADQLFIQNCKDILENGVWDTDHEVRPVWEDGTPAHTIKRFG
                     IVNRYDLTKEFPVITLRRTAFKSAVDELLWIWQKKSNNIHDLKSHIWDSWADETGSIG
                     KAYGYQLGVKHHYKEGEFDQVDRILYDLKHNPLSRRIMSNIYNHHDLSEMNLYPCAYS
                     MTFNVSGNTLNGILNQRSQDMLTANSWNVCQYAVLLHMFAQVSGLKAGELVHVIADAH
                     IYDRHIPMVEELIKKQPFEGPTLWVDPEVKDFYQFTTDSFRLENYQFHPFEHKIPVAV
                     "
     gene            49624..50721
                     /locus_tag="ACZPCG_00225"
     CDS             49624..50721
                     /locus_tag="ACZPCG_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HIR1283531.1"
                     /translation="MTQKRIRDYGIMPGRIKTGKRNKITDVPGVRVGHCTVKEGEIHT
                     GVTVILPGPDNAFLNPYTAAAYVHNGFGKSCGLIQIQELGTLETPIALTNTLNVGKVW
                     DAVAEYVLRQCENDGAEALSINPVVGECNDSRINRISLRAIGAGQVKKAFDAAGEDTE
                     EGDVGAGAGTICYGLKGGIGTSSRVIKVGEKEYTIGVLVQSNFGATEDLMVDGRPLGR
                     EILEKYGNEAEDPVKKTAFSEQDKGSIMSILATDLPVTDRQLTRIIRRLGVGIARTGA
                     YTGHGSGEVMIGFTTANRVPTKGLRREEEELRTLTAIPEETINRAFLAAAEAEEEAIL
                     NSMAAAGETRGLKGEYYRSLSDILSQEGEIH"
     gene            50748..53726
                     /locus_tag="ACZPCG_00230"
     CDS             50748..53726
                     /locus_tag="ACZPCG_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006781460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HIR1283532.1"
                     /translation="MTLQEKAALLGGKGEWETRDIPRLGIPSMVCSDGPSGLRRQAGT
                     GDHLGLNPSLPATCFPCPSLMACSWDQELEEAIGAALGEEAADQGVDILLGPGLNIKR
                     NPLCGRNFEYFSEDPYFSGKMAAAFIRGVESQGIAACPKHFAVNSQELRRMTMNAVVD
                     ERTLREIYLTGFETAIKEGKCRALMTSYNQVNGIYANEHPHLLKEILRKEWGFCGAVI
                     TDWGGSNDHCAGVKNGSSLEMPAPGLSSAAALLKGVEEGRITEQDLDERVEEILNLLP
                     GQKKMFKQNKMPENSPEREPLYEAHHHLARRACAESVVLLKNETDTLPLKSGTRIAVI
                     GDFAYESRYQGAGSSMVNAWKVDSVKECLEGETGLEWKKMGITVSGFSRGYLRDGRQD
                     AALEKEAVDLAKTADQILFFFGLNEAWETEGMDRSHLRIPENQIHLLERLSRLELPVV
                     GILCTGSVVEMPWEGHLKALLYGGLWGQAGAGAVLDLLSGKLNPCGKLAESWPVKYED
                     TPSFQYYPSKERNSEYREGIYVGYRYYDTAGVPIRFPFGFGLSYTKFDYSGLKITPEG
                     VSFVLENTGSRDGAEIIQLYIGLPGARIFRPKRELKGFCKVFLKVGEKKEVFLPFDSM
                     TFRYWNGRTGQWEREGGFYRISIGSGLTDIRLEGEVFQEGTTDSLPWEVNELPSYASG
                     RIRSVPDEEYERLLGCPIPDGSWKGELGINDALCQMYYAKSPLARLICRILKHRLENA
                     QKKGIPDLNTLFQYNMPFRAMAKMTGGWISMEMAESLTYAANGRHFYGITGFIRGFLK
                     NRRLERRYEQALKGKTAPNVGNPWSKAHPQLWEFILFNLLSNCATITNFVVMWLCTGW
                     VFKGLADIPFRFFIFDYGEDALGLCGFLSFLTATALAQMVNFFVQKNWVFRSDAAFSR
                     AAPRYILLAVFLVILSAALPAWSQKLFTGLGVPEAFAPTCANGLNILAQVAISYPAMK
                     FWIMPGQARPKKNKKKRRKK"
     gene            53726..54763
                     /locus_tag="ACZPCG_00235"
     CDS             53726..54763
                     /locus_tag="ACZPCG_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HIR1283533.1"
                     /translation="MQFEKYTEYAVEQAKKLLAIDSPSGYGYYVTEYLLKEFAALGIP
                     ARRTIKGGVIADFGGKKDENGILLEAHCDTLGGMVSQIKENGRLKLTNIGGMKPANAE
                     AENVRIITKGGNVYEGVCQLVNASVHVNGNYETTERSWDTVEVLVDECVKTKEDTVKL
                     GIMAGDYVCFEPRTRVTASGYIKSRFLDDKLSAAILLAYGRYLKEEKVVPERHVYAHF
                     TIYEEVGHGGSASVPEGVTEAWSVDMGCVGDGLTCTEHEVSICAKDSGGPYNYEVVCR
                     LVELAKEHEIGYAVDVYPHYGSDVETTLRSGHDIRHALIGPGVYASHGYERSHKDGVC
                     NTFHLIQAYLG"
     gene            54826..55815
                     /locus_tag="ACZPCG_00240"
     CDS             54826..55815
                     /locus_tag="ACZPCG_00240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HIR1283534.1"
                     /translation="MDRRILFILIMSLAVPYGCSMALLGTSLAKSGGLKEQEALSAAW
                     ASDSREKRSFRRIRMDMEDPPEKTVDLETYLPIVVAGQISAESHPEAIKAQTILARTY
                     ICRQMEQMGLEEEIPESSLGLEKNRNNQMEPLWGTEIFPEYYSRFEKAARETEGVTLT
                     WEGHFIDPLFTHTTTGFTRPGDEEHPYLASVSCVHDPEEEGFVQEMIMQPEEAAKRVS
                     AIPAGDGSFRTIPPEAFPEQVQIISRDEAGYALQLQIGGFPFNGEEVRLALGLPSACF
                     ELNRHGSDVRFTVRGRGHGWGLSQTEAQALAEDGWSAEAILEYFYTGISFSSE"
     gene            55858..56634
                     /locus_tag="ACZPCG_00245"
     CDS             55858..56634
                     /locus_tag="ACZPCG_00245"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC family protein"
                     /protein_id="HIR1283535.1"
                     /translation="MKEKVNQMLKDKLFLVMLVLGLLTMVAAAGIMTVQKGKNSANDP
                     MLHMAEEENTPTEEALQMAGASDAREKTQEEDREETVGADAGENPNEGNDLLAEAKEQ
                     DGAAEAGAGKEAAHALALNFTESSSMVWPVRGNVLLDYSMDQTIYFPTLDQYKCNPGI
                     VIQSDVSAPVAAPANARVTEVGVSDEIGNYVVLDLGNEYTAVCGQLKEISAAEGEYLE
                     AGQTLGYVAEPTKYYSVEGVNVFFELKHKGETVDPLNHME"
     gene            56798..57319
                     /locus_tag="ACZPCG_00250"
     CDS             56798..57319
                     /locus_tag="ACZPCG_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR1283536.1"
                     /translation="MNILFFLTPKSEVAYINEDDTLRQALEKMEHHRYSAIPIISRTG
                     RYIGTLTEGDLLWGIKNQYHLDLKGAEKIPITAIHRRLDNRPVKAGADMEDLMGKALN
                     QNFVPVLDDQKCFIGIITRKDIIQYFYKKTQQAQEDVKACCGSGYAENKKRDVASAVA
                     FQSEKERANEKTM"
     gene            57679..59019
                     /locus_tag="ACZPCG_00255"
     CDS             57679..59019
                     /locus_tag="ACZPCG_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006774200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR1283537.1"
                     /translation="MKPYAEMSKEELTALRRELKAKYHEFQTRDLKLDMSRGKPSVDQ
                     LDLSMGMMDVLSSDDDLTCEDGTDCRNYGVLTGIDEAKELLADMMEVNPSTIIIYGNS
                     SLNVMYDTVSRAMTHGIMGNTPWCRQDKVKFLCPVPGYDRHFAITEYFGIEMINVPMT
                     SEGPDMDMVERLVASDPAIKGIWCVPKYSNPQGYSYSDETVRRFARLEPAAPDFRIFW
                     DNAYGVHHLYDHDQDHLIEILAECKRAGNPDLVYKFASTSKISFPGSGIAAIATSHNN
                     LEDIKAQLKNQTIGHDKVNQLRHVRFFKDIHGMVEHMRKHADIIRPKFEAVEEILERE
                     LGGLGIGEWTKPKGGYFISFDSLDGCAKDIVARCKKAGVVMTGAGATYPYGKDPHDSN
                     IRIAPTYPPLGDLIVATELFSLCVKLTSAEKLLREMEQNPQPVKKKEADQQLPE"
     gene            59156..59758
                     /locus_tag="ACZPCG_00260"
     CDS             59156..59758
                     /locus_tag="ACZPCG_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1283538.1"
                     /translation="MEEAGVYTGKWDFVTFFVVPGATLWLACREDWLDSNISVIGNSP
                     GMELWLYAWGMFVSSYSFFYIRYLFSLGGYEGIKGRGFLYTACILLAISVWIPYDPEH
                     FPKASLLHVIFSFSFPVLLSLSLLSFLKEKIRSDPNPFLPILWLFAELAAAALVLWYA
                     SGFITGLLEVYVTTAFCGYLGLVERILVHNQRSFPENRGD"
     gene            complement(59755..60942)
                     /locus_tag="ACZPCG_00265"
     CDS             complement(59755..60942)
                     /locus_tag="ACZPCG_00265"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIR1283539.1"
                     /translation="MRFTLPRDLYHGKDALEALKTLTGKKAIVVVGGGSMKRFGFLDK
                     VVAYLQEAGMEVQLFEGVEPDPSVETVMKGAKAMEEFQPDWIVAVGGGSPIDAAKAMW
                     AFYEYPETSFEDLITPFSFPTLRTKAKFCAISSTSGTATEVTAFSVITDYAKGIKYPL
                     ADFNITPDVAIVDPALAATMPKKLTAHTGMDAMTHAIEAYVSTLHCEYTDPLALHAIE
                     MINEYLVPSYNGDMDARAKMHDAQCLAGMAFSNALLGIVHSMAHKTGAAYSGGHIVHG
                     CANAMYLPKVIKFNSKNPEAAERYAKIAKFIGLAGSTDLELTDALIARLREMNEQLDI
                     PACIKDYEGGIIDEAEFMEKLPKVAELAVGDACTGSNPRSIDPEQMAKLLKCCFYDEE
                     VTF"
     gene            complement(61111..62037)
                     /locus_tag="ACZPCG_00270"
     CDS             complement(61111..62037)
                     /locus_tag="ACZPCG_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1283540.1"
                     /translation="MNLSYLKYAVEVEKTGSITKAAQNFYMNQPHLSKIIRELERELG
                     EDIFDRTSRGMIPTKKGEEFLRYAKTIMAQEEQIEALCARNRKQALELKFCGPRATYI
                     TCAFTDFLCEANTKSPVNIHYMETNSRQVIELVSSKEFDLGIARCPALYEPYYKKLLQ
                     DENLDSCLLWEFSSLVLMAPDHPLAGRSGLTYLDLGSSLEIIQGDSSNPSFSFEIQET
                     SATGGDSSRSVSVYDRGSQFALLRRLPGAYMWTSPIPYSELSAEGLIQRPCAYPGNLH
                     RDLLIFRKGSRRSSAETELIHSLNRIVSRLSD"
     gene            complement(62055..63683)
                     /locus_tag="ACZPCG_00275"
     CDS             complement(62055..63683)
                     /locus_tag="ACZPCG_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HIR1283541.1"
                     /translation="MTNHYDHRRNKASRSSYRGPRKSRRPASETEKGKFLLPLAAAGA
                     GIFLLAAVSAVTISHFKGKPAAGSHETSLSETDASLTAPAVTVNGISLNGLSPDESFE
                     ALNQLFLWDMKAEYGEEQLAVENPLSSRLKEWIGTLCRSSQSGDLTRTPADLINEELK
                     KEILIQAETFAKKWETAPKNSGLDYFDPQKGSFIFTEGCSGIEIDERRLAEDILSAIR
                     ENRLDAVIPVHSADAAPPLSKAMAKEQYKTIAAFTTETTSNQKRNTNVRLAAEALNGT
                     IVQPGEEFSFNQAVGQRTAEKGYQEAAAYSSGAVVQEIGGGVCQISSTLYRAAFNAGM
                     EITFRRSHTFEPNYVTPGQDATISWEQPDFRFVNTSSAPIGIRASYADRKATVSIYGI
                     PVLEEGVSWDLYYEKVEELPPPEPEYAEDPSLEPGTEKVVKAAVPGSRWDTYKVISKD
                     GEERERILDHSKTYKGHAAVIHRNTASSETSKAEQTPSAAPYETKTGPEHPASSDPAP
                     AETLPSDAPFVDGMPDSYTPKTNPIIPFQPTASS"
     gene            complement(63782..66100)
                     /gene="feoB"
                     /locus_tag="ACZPCG_00280"
     CDS             complement(63782..66100)
                     /gene="feoB"
                     /locus_tag="ACZPCG_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006568797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HIR1283542.1"
                     /translation="MNPFLSLNDIQPGQTARVTELLVSGAMRRRLMDLGLTADTQVEC
                     LGVSPLGDPVSYRIRGAVIAIRREYSRQIRVDGNENKNQFSVALSGNPNVGKSTLFNS
                     LTGSSQHTGNWAGVTVSCFKGSCSSKKYNYVFADLPGTYSLMAHSAEEALARDGICFG
                     NCDAAVVICDSTCLERNLNLVLQILETGIPAMVCVNFMDEAAKKNIRIDLPALSRELS
                     APVAGTTARKKRSLEHFLDRLDETVDHPDTEPFHISYDAAVEQAISMLLPFTEPLSSR
                     LSPRWLALRLLENDPGLLRSLNTFLGRDLSEDDALCQAVLQARSHLDQAGIFEDDLKD
                     RIVSGIVNTAESIASRTVSLPEKPYGKRDAFWDRILTGRLAGYPAMIGLLALIFWLTI
                     TGANYPSALLSSLFFSFQEKLTAAFMAVNAPQWLHGILVLGVYRVLAWVISVMLPPMA
                     IFFPLFTLLEDFGYLPRIAYNLDRPFQCCKACGKQALTMAMGFGCNAAGVTGCRIIDS
                     PRERLIAILTNSFVPCNGRLPALVTLITLFFTGASSAGPISSLRPALILTFFILTGVA
                     ATFFVSRLLSATVLKGMPSSFTLELPPYRRPQIGRVIWRSILDRTLFILGRAAAVAAP
                     AGLVIWILANVSIGDISLLARFSGYLDPIGRLMGMDGVILMAFILGLPANEIVIPIII
                     MAYTSQGTLIEPGNLSELHTLLISQGWTWVTALCTMVFFLFHWPCSTTLMTIKKETGS
                     LKWTCLAVLLPTACGVILCMAITAFSRLLTFL"
     gene            66227..66721
                     /locus_tag="ACZPCG_00285"
     CDS             66227..66721
                     /locus_tag="ACZPCG_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007709103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6106 family protein"
                     /protein_id="HIR1283543.1"
                     /translation="MEESYIEWLVKRKDPIYALPVKIIMVLVCAFSVLLALQTVIGVV
                     IMTAAGIGTYFIFLNLSVEYEYLFAEGGLRIDRILGKARRKKAFDCEKDDVQLIAPVD
                     SYMLKDYAKQGMKVMDCTSGKQGAEVYALMYQKGADTMKVLIEPNEKMLAAMRHTFPR
                     KFVK"
     gene            66879..68333
                     /gene="guaB"
                     /locus_tag="ACZPCG_00290"
     CDS             66879..68333
                     /gene="guaB"
                     /locus_tag="ACZPCG_00290"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015554889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HIR1283544.1"
                     /translation="MGTIIGEGITFDDVLLVPSYSEVIPNQVDLTTYLTKKIKLNIPL
                     MSAGMDTVTEHRMAIAMARQGGIGIIHKNMSIQEQAEEVDRVKRSENGVITDPFYLSP
                     EHTLKDANDLMAKFRISGVPITEGKKLVGIITNRDLKFEEDFSRPIKECMTSENLVTA
                     KEGVTLKEAKAILAKARVEKLPIVDDNFNLKGLITIKDIEKQIKYPLSAKDEQGRLLC
                     GAGVGITANVLERVAALVEAKVDVIVLDSAHGHSANVIRCVKMIKDAFPDLQVVAGNV
                     ATGEATRALIEAGADCVKVGIGPGSICTTRVVAGIGVPQITAVMDCYNVAKEYGIPII
                     ADGGIKYSGDVTKAIAAGGSVCMMGSMFAGCDESPGTFELYQGRKYKVYRGMGSIAAM
                     ENGSKDRYFQTDAKKLVPEGVEGRVAYKGMVEDTVFQLLGGLRAGMGYCGAKDIPTLQ
                     NTARFVKISAASLKESHPHDIHITKEAPNYSIDE"
     gene            68515..69477
                     /locus_tag="ACZPCG_00295"
     CDS             68515..69477
                     /locus_tag="ACZPCG_00295"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HIR1283545.1"
                     /translation="MIAACLTGCQKHREPVSRSSFLLNTFITVTLYDSREEEILDGVT
                     ELCSEYEKKFSTTIETSEIYAMNHRKPGEREFTVSQDTAELIEKGLEYSRLSDGAFDI
                     TIEPLSRLWDFSGSDPHVPSEEAIRKELLKVGYERVAVNGNTVVFADDLVSIDLGAIA
                     KGYIADRMKEYLKEQGVESAIINLGGNVLCLGERPGNKPFKIGLQKPFAERSETVAAL
                     DIRDMSVVSSGVYERHFEENGVNYHHILNPSTGWPYENGLTQVTILSEQSVDGDGLST
                     ACFALGLKEGMELAQSLEGVYAVFVTEDGTLHYSKGAEAFISSP"
     gene            69558..70004
                     /locus_tag="ACZPCG_00300"
     CDS             69558..70004
                     /locus_tag="ACZPCG_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HIR1283546.1"
                     /translation="MFEAIWKKCVNYETISYLICGILTTAVDFVSYAMFRRMDMGVGM
                     AQAFSWGAAVLFAYVVNKLIVFKNYDLRPSYLMKEAGAFLMTRGISGVAVWLLMVVMV
                     ALGEGRGFLYEMFCKMVSSAVNLVANYIFSKLWIFKKRSESKGKQQ"
     gene            70001..72706
                     /locus_tag="ACZPCG_00305"
     CDS             70001..72706
                     /locus_tag="ACZPCG_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HIR1283547.1"
                     /translation="MKREVIVQAERELDAVLELIQESEGISGRDDIKEERMKERNRIQ
                     EAEVEESLTRINMEDTADMLQYEACRELKRCRDVHILRPGDSLLAAFFVPVLVLVVIF
                     AQRGIFPFGEECFLRTDMYHQYAPFFSEFQYKLTHGGSLLYSWDIGMGVNFSALYAYY
                     LASPMNWLVALCPRQFLIEFMTVLIVIKTGLSGLFFTWYLRKHTGTREFGSCFFGIFY
                     ALSGYMAAYSWNIMWLDCILLFPVILFGLERLVKEKKGMVYCIALGLSILSNYYISIM
                     ICIFMVIYAAVLVILYPPKKGKEFAATAGRFTLYSLLAGGLAAVVLLPEIYALQATAS
                     GNFDFPKTVSSYFSIFDMIARHIGNVQTEIGLDHWPNIYCGVAVLMLLLLYLGNKNIK
                     IKEKAVYFSLLLFFYASFSVNVLNFIWHGFHYPNSLPCRQSFIYIALVLVMCYKAYLE
                     LKNTPWKHIVMAFWGAAAFVILAEKLVDNEEQFHFAVFYAAILFLALYTGCIYLYHSR
                     KWCRDGVLLAVLGLVFCESAVNMAVTSIPTTSRTAYVKDNEDTMLLADSIRSSVFYRI
                     EKGESRTKNDGAWMNFPSASLFSSVASAAMSDFFKSVGCESSTNAYSVKGSTPFIDAL
                     FATRYGIYPDQQPADGLKEQIGRQGSMWFYENKYTLPVGFVMPQDMETNWVLDSGNPA
                     NVQNDLSSVLGVSNLLVPAEGVSEGKKFTFTADASGDYYVYVTNKKVEEVSAEIGERS
                     LSFDNVDRGYFLELGYLPKGQEVILQSKTDGNPAMQAEIWRFDPEAMEEIYQCLSKSP
                     LELSSWTDTGLAGSINTPEGGTMFTSIPYDKGWKIWVDGTAVSGRPVFDAFLGVDLEP
                     GEHKIRLSYEPQGLKTGAVITGVSAAAVAALAVCGWMKNKKKFLK"
     gene            72732..74108
                     /gene="argH"
                     /locus_tag="ACZPCG_00310"
     CDS             72732..74108
                     /gene="argH"
                     /locus_tag="ACZPCG_00310"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - L-arginine biosynthetic process
                     via ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HIR1283548.1"
                     /translation="MKLWGGRFTKETDQLVQNFNESLSFDQKFYRQDIRGSIAHVKML
                     AKQGILSEKDRDDIIKGLEGIRQDLDEGTLIIPAGSGYEDIHSFVEGTLTERIGEAGK
                     RLHTGRSRNDQVALDMKLYTRDEIDELDGLVKKLLEELLVIMEANTETFMPGFTHLQK
                     AQPITLAHHMGAYFEMFSRDRSRLADIRRRMNYCPLGSGALAGTTYPLDRSYTAELLE
                     FAGPTLNSMDSVSDRDYLIELLSALSVISMHLSRFCEEIIIWNTNEYRFVEIDDSYST
                     GSSIMPQKKNPDIAELIRGKSGRVYGALMSMLTTMKGIPLAYNKDMQEDKELTFDAID
                     TVKGCLALFTGMISSMTFRKDVMEASAKRGFTNATDAADYLVNHGVPFRDAHGIVGQL
                     VLLCIEKGIALDDLSLEEYKEISPVFEEDIYEAISMKTCVEKRMTIGAPGPEAMKQVI
                     AVYKKQLQ"
     gene            74172..76337
                     /locus_tag="ACZPCG_00315"
     CDS             74172..76337
                     /locus_tag="ACZPCG_00315"
                     /inference="COORDINATES: protein motif:HMM:NF012772.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIR1283549.1"
                     /translation="METIRKRSRIFTICAVLVAMFCLAVGAGNTVSFERMMNQVVERY
                     VSENNQQVASQISYRLKMGREFVSDFADTLSRMPQFLLTEDFISRKTAAVELEGLMVL
                     FEDGSVFPESACPDYLSQWFEKNPDIWEKQVTSYVADQNLIFTAPVHYGDGTEAVVVG
                     LQNYWSIKALVFRADYQEQGASFLIDRKDGEILIADEKSALSVTDQQLYALLETGREL
                     EGDRQVYEAVMPNGQEVFVSLYEVRDTDWVQAAVIPSDFLMAQIERHMDVYAAVVVAV
                     MVLLGVLIGHLMKENQRWERLSVTEPLTGGWSREGFIKLGTQNTEGHRLHEWIVVYLN
                     IKDFRHINESWGEEDGNLMLQFIYRMLSGLINEEELVSRSSMNHFFLLLKEEDDREVN
                     RRILKMKEAVNERIRCKFSEYSIDFAVGACRLDIAGGVPTAMNKAIYASKLSDTDNVC
                     SFYNAGIANMLEREERLNTLFEDSVKNRDFKVYLQPKVSPSETRACQAEALVRWFHPQ
                     EGVIYPSEFIPIFEKNGKICSLDLYMFEEVCRLIDRWIKEKKALVSISVNVSRFHLRE
                     AGSEIWKEYKRILDRYDIPEGVIEMELTETILLEENQIPFVKAVLNGFRSCGLRVALD
                     DFGFAYSSLSMLKEFAVDTLKMDRSFFVNENDKSRRIVGSIIQLAHSLDMDVVAEGIE
                     AKEQVEMLRKMGCDLIQGYVYSKPLPVGEFEQWREEYDR"
     gene            76375..78258
                     /locus_tag="ACZPCG_00320"
     CDS             76375..78258
                     /locus_tag="ACZPCG_00320"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR1283550.1"
                     /translation="MILILIILILPTNVALQLYIQHQNQKQNSEEIFWQLEQLIRNNI
                     YDLEREKRDFSQKCIQSAKMAAYFVEHNPGYITDLEHTKELAEKLDVDEIHFIDPQGE
                     IYAGTHPRYYGFTFRDGEQMQFFLSMLEDTSLELCREITPNTAEGKMMEYAAVWMEDK
                     SGIVQIGMEPKRLLKQMEEKSLKKVVSEIPSGLMGYLHIINTNTGKIEASTVPGMNGL
                     DIHEELKTEDKIKAPASGHYRYNGRKFCVYTKYYGQYLLVRSFVSAYPLRSAVQSTLL
                     VFLYILGASVAVLWIIMWYMKVKLAGNLTAIVGELKKIEGGDLTNLTIHTGILEFDEL
                     VQYINALLKSIRLNWDKMDHVIENSRVPVGVFEYNHFYKKTFMNNRILKILGISDADE
                     NNDALSNRIRKKLEEAENHPVDAGKSLYEYDRNGETVFARIEKFTDEQSTTWCVTDVS
                     QWWNEINRLREQSSRDSLTLLYNRRGFTERLEALFADPGAIGSGVMIMLDADGLKGIN
                     DMYGHHMGDVYLRQIGRQIEFSGEKRFISARMGGDEFSAFLYGYASKEEAEKVLRDFE
                     AHRGEVFMDPEMKHILGTVEFSVGYAFYPEDGTDYHVLMHISDERMYEEKKKRKGRRS
                     REE"
     gene            78255..79388
                     /locus_tag="ACZPCG_00325"
     CDS             78255..79388
                     /locus_tag="ACZPCG_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0004529 - DNA exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HIR1283551.1"
                     /translation="MKLFHLSDLHLGKRVNEFSMLEDQQYILSRILEWVHKEKPDVLI
                     LAGDIYDKAVPSAEAVSLFDWFLTELADRACPVCIVSGNHDSAERLAFGARLMNARNI
                     FLSPVYEGKVETVDFKDSFGRIRIHLLPFVRPLSVRHAFPKEAEGIVDYQTALRTAVE
                     HMELLEGERNVLAAHQFITGAMSCESEEVTVGGLDNGDASLFDAFDYVALGHIHSPQS
                     VGRKEVRYCGTPLKYSFSEVSQEKSITVVELEEKGNVQIKTLPLIPLRDMRKIRGTYM
                     EVTDRAFYEGTDQQDYIQVTLTDEEDIPDGLQKLRTIYPNIMRLEYDNRRTRKDAEIR
                     EWEKQEELSELELFSRFYEQQNNQPMSPEQEAFVKHIMEEIKE"
     gene            79392..>80306
                     /locus_tag="ACZPCG_00330"
     CDS             79392..>80306
                     /locus_tag="ACZPCG_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR1283552.1"
                     /translation="MKPEKLIISAFGPYAEEIEIDFEKLGDRGLFLITGDTGAGKTTI
                     FDAISFALYGEASGEIREAGMFRSKYASEQTPTFAELTFSIQEKRYRVRRNPEYLRPK
                     GRGTGFTVQKSDAELIFFDGRPPIVKNKEVTKAVTELLGLDYRQFTQIAMIAQGDFQK
                     LLLAGTAERSEIFRRIFHTEPCQELQNRLKYAVKERWKDYDEMRRSISQYLDGVQCGC
                     APEIDAEFKTLKKEHFDGKAVRGMELLSRLLEMDKNSLFQLDKEIRGLEEEIQEQDQL
                     LGKAEQRRRLVQELKLRETEERELAPVLE"
BASE COUNT        21858 a        16229 c        22237 g        19982 t
ORIGIN      
        1 gagaaacgag agttgcataa atacggtgat tcgtacaata tgtacaaaaa cattatatat
       61 aaattgtgta aaatattaga atataagaaa ataaataaaa aagtattgac aggaatcatc
      121 aaaaatacta tactcaagcc ataggtaaac caatttacca aaagcaacag atacagctgt
      181 acgggatatc acaaagtaaa aaagaaatac gaatggtaaa caaaagtaaa cctgttcggg
      241 gaggacaggg tgactgttcg cagattaagg attgacagat ctgtgacaga ttcagaaagg
      301 atgagggaaa aatgacaggg attatcataa caggacatgg ccattttcca accgggcttt
      361 taagcgcggt aagtctggtg gcaggtaagc ctgaaaaggt agaagccgtt gattttacag
      421 agggaatgag ctccgaagaa ctgaaagggc agttaaagga ggcagcagac cgtctggagg
      481 aacaggagat tctgatttta gcagatctta ccggaggaac gcctttcaat atggcagccg
      541 ctgttaagat ggaagagaca gagaagcatt ttgcagtagt ttctggtgtc aatatgcctg
      601 cgcttgtgga aggtgtattt tcaagagtga tgtatggttc tttagaagag ctggcagcag
      661 gtgtgatgaa ggcgggaaaa gagggacttt gcagtctgga agctctggca gacgaggaag
      721 aagaggaagc gcctgagttt gaggacggcc tgtaagatac cggggcaaaa gccggaagat
      781 aaggaggagg acgaagtgat aaagacgatt caggttgaac cgattaagaa tcgggagcat
      841 tttttgaata tgcccttcct tacaaaggaa gaggtaaaaa cagccatgga ccgggtagtg
      901 aagcagacag agctgaatat ggagtatttc ggcacacgct tcccatggcc agctgcaaaa
      961 cagggtacat atgagatcat tgacaacatc gaatggacag acggcttctg gaccggtctt
     1021 ttatggctct gctacgagta caccaaggat gagaagttca ggaaacgtgc tgaggaaaat
     1081 atggcttctt tcttaaatcg agtggagaag cgtattgaac tggatcacca tgatctggga
     1141 tttctttaca ttccttcctg tgtggcagga tataaactga ccggttctga gacaggaaag
     1201 cgggctggaa ttctggcggc agacaagctg atggagcgtt ttcaggaaaa gggaggcttc
     1261 attcaggcgt ggggagcact gggagccaga gataattacc gtctcattat tgactgtctt
     1321 ttgaatatcc ctctgcttta ctgggcaacg gagacaacag gagataagaa gtatgcagac
     1381 gcggcaaagc gccactatga aacagcctgc aactatgtaa tcagagacga tgcttccgct
     1441 tatcatacat tttactttga cccggagaca ggggctcctt taaagggtgt aacacgtcag
     1501 ggctatagtg atgattctgc atgggcaagg ggacaggcct ggggtatcag cggtattcca
     1561 tttaattacc gctacacaaa ggacgaaagc gcatttaact tgtttgaggg catgacgaat
     1621 tatttcttaa accgtcttcc ggccgatgat gtgtgctact gggatctgat cttcggagac
     1681 ggctccggac agtccagaga ttcctcagca gcggctgtgg ctgtgtgcgg tatacatgag
     1741 atgatgaagt atctgcctga gacggatgaa gagaaggaaa cctaccggag agccatgcac
     1801 ggcatcctgc gttctctgat tcagggatat gtaaatccgg atatgcatcc gggagcacct
     1861 atcctgcttc atggcgttta ctcctggcat tccggtaaag gtgtggatga gggaaatatc
     1921 tggggagatt atttctatat ggaagcttta ctgcggtttt ataaagattg gcagttatat
     1981 tggtaaaaaa tggaggattt aaaaatggca gaaccaaata ttgtagcatt tcgtattgat
     2041 gagcgtctga tccacggaca gggacagctc tggatcaagg ctttaggtgc aaatacagta
     2101 atcgttgcag atgacggcgc aagcgaggat cctatgcagc agatgttaat gaaggcagtt
     2161 gttccaaaga ccattgccat gcgtttcttc tctgttcagc atacctgtga tgtgatcatg
     2221 aaggcttctc ccaagcagac cattttcatt gtctgcaaga ctccggcaga tgcgctgcgt
     2281 ctggtaaagg gcggcgttcc ggtgaaggag atcaatgtgg gaaatatcca cagagctcca
     2341 gggaaagagg agatcagcaa atatatcgct ctgggcgagg atgacaaggc agctttaaga
     2401 gagcttcgtg attcctatgg aattacattt aacacaagag cgacagcatc cggtgacgac
     2461 ggttccggaa aggtcgatct gaataagtat ttataaagga tacaggaggg aaaaaaagtg
     2521 actatttcaa ttacgcagtg tgttttgatc ggtctgtgga ccgcattctg tttggcaggt
     2581 atgctgttcg gtatttatac caaccgttgt ctgatcatgt ctgcaggcgt gggactgatt
     2641 ttgggagatc ttcctacagg acttgcaatg ggggctgtcg gtgagcttgc atttatggga
     2701 ttcggcgtat ctcagggcgg atctgttccg ccaaacccaa tgggtcctgg tattgtggga
     2761 accattatgg caatcactat gaaggattcc ggcattgata caggttctgc cctggcatta
     2821 tccttcccat ttgcagtagc attccagttt gtgattacag ctacctatac attcgcaacc
     2881 acacttaccg gtctggccta caaggcatta gaccgcaagg atttcagagg tttccgtctg
     2941 gcagccaacg ctactgtatg tattttcgct ctggtaggct ttatcattgg ttttggaggc
     3001 gcattcagcg cagaaggact tcagaaggtt atttctctga ttcctgcatg gctgagcacc
     3061 ggtctgggta tggctggaaa gatgcttcct gctattggtt ttgcaatgat cttaaacgta
     3121 atggcaaaga gagagctgat cccattcgta cttttaggct acattgcaat tgcttatatg
     3181 aagcttcctg taatgggcgt agcgatcctg ggaacagcaa tcgcgctgct ggtatttttc
     3241 aatgctggca gaaagggtga agattcagta gaagaagtgg aggttgagtt tgaagatggc
     3301 atctaatcgt ttggaaaaga aagattattt aaaaacatcc ctgcgtgcct ttttcctgca
     3361 gaacggattt aactacggaa actatcaggg ccttggctat gccaatgtaa tgtatccggc
     3421 tctgcgcaag ctgtacaagg atgacgatga taagcttcag gaagcattaa gagaaaatat
     3481 tgagtttttc aataccaata tccatttcct tccatttatt accagtcttc atctggttat
     3541 gctggaaaat aagactcctt ccaaggaaat ccgtaacatc aagatggctc ttatgggacc
     3601 gctggccggt atcggcgatt ctctggctca gttctgtctg gcgcccctct ttgcaaccat
     3661 cggcgcatcc ctggcacagg aaggcctgat catgggaccg atcctgttct tcctgggaat
     3721 gaacctggtg ctgttagcct taaagatcgc taccggtatg tggggatata aggtaggaac
     3781 cagcattatc gaaagcctga gcacccgtat ggagcagatt tccagcgtgg caagcatgat
     3841 cggcgtaacc gttatttccg gactggctgt aaacttcgta aagattacaa cgccaatcca
     3901 gtatgtggcg cagatgcctg ataaccagca gaagatcgtt gcaattcagg acatgattga
     3961 cgctattgcg ccaaatctgc tgcctgtatt atttacaggt ctgatcttct acctgatcaa
     4021 ggtaaagaag tggaccactt acaagttagt tgttttaacg atcatcatcg gcgtggtact
     4081 gtcctgcctt catgtgatcg cataaaaaag gattcaaaaa ggggagattt acccagggga
     4141 gacgcatttc ggcggctccc ctgttggtgc attaaaagga gaaaaaatga cagaggaaag
     4201 aaagaagatt ctcgccgccg tgacagaacg ggcagagaat tttgtaaaag gatttgatcc
     4261 ggagtttgca gccggttata tgaaaaaacg ggaggaagca gaggcggaag agcttcttat
     4321 acgggccggg gagctgatgg atcagacctt tgtgtttgca gataaatggg atatggagcc
     4381 atgcagagag ccctataccc tgacagagat ggagtggcag aggacgccaa acggggatcc
     4441 ggagtggata tttatgttaa accgccacga ttaccttcat aagcttatga tggcctatta
     4501 cctgacagga aacgaggctt atacagataa gctgaagtgg tatctgtttc actggatgtg
     4561 tcataatccc atactgcctg aaggctctga cagcacccgt accatagata cgggaatccg
     4621 ctgtatgaac tgggaagatc tgatcctcca tctggcagga aacgggatgc tcacgcagga
     4681 ggagctggat gagcttctct taaagctgga tgagcagttt gaaaatttaa gacagcgtta
     4741 catcgggaaa tataccttaa gcaactgggg gcttcttcag acaacagcga tctgcgaggg
     4801 ttatctgctg ttcggagaca gtctttgtca tcctgatacg ggaaaatggg cctggcagga
     4861 gttaaagcgc cagcttgatt tgcaggttat ggatgacggt tcccactggg agcagtctgt
     4921 catgtaccat atggaggtac ttttggcctc catgcggctg atgaaatgga aagaactgga
     4981 agaaaataat ctgtgtccag agagatacag aagcgatttt ccggaggaat gggattggct
     5041 gaaagccctt atagagaaaa tgagcctcta tgtgctttat tgtgccggtc cagatcacag
     5101 gcagccggcc cagtgtgaca gcgatcggac tgatgtaagg gacattatgg taaaggcggc
     5161 tgttcttact ggaaatggcg tatttcggtt ttgcggatat gaggctctgg atctggaaag
     5221 cctgtggctt tttggacgaa agggagcgga gcggtatgaa gcagctgtat ccagggagcc
     5281 ggagcgcctg aactatcatg cccaggatac aggtaatatc tatatacgga gcagctggaa
     5341 agaggacgca aactacacat acctttcctg cggccctcag ggaagcggtc atggtcatgg
     5401 ggatctgacg cacatttccc tgtactatca gggaaaacca tttttgattg acagcggccg
     5461 gtattcctac cgggaggatg aaccgctgag gatggagttt aaaatgcttc aggcccacaa
     5521 tgtctgtgtg gttgacgacg gttctgtgat ccgtccggac ggttcatgga gctttcattc
     5581 ctacggggac tgttttaaga catattaccg ggaaaaggaa gggatctcct actgcgagat
     5641 ggcttaccgc ggaaggcttg caagcgggga aggctgtctg gttacgagga aggttatgac
     5701 agccagagag gggctgtggc tgattgtcaa tgatatctgg tgtgagggaa agcatacggt
     5761 aagagaatat taccatcttg atcccggtgc agaagcaagg gtggcagaag atgagattct
     5821 ccttacaaac ggatctgtac agttaaaggc gctgggagag ccgcaggagc atgggccatg
     5881 gacccagccg gttccgattc catttcagct ggaaagctgt aaggtttcac tggagtacaa
     5941 ccgactttcg gatagtatgt gtctggtaaa agaaggaagc ttttcagact cttttgtaag
     6001 cagcagtgcc ttctgtgggg ccggagtaca ggtttcggct cctgaagtat atcaatttgg
     6061 ggccggagag ccggtatcag acggccgtgt cactgccaaa actttccatc tgtcagagga
     6121 agaatcctgg acctttttag tatggaaccg tgagaccttc cggggtggaa agatgtatga
     6181 gtgcagcgga gtccctgttt atggaaaggc gggagctatc cacagccttg gaggaaaaca
     6241 gacctggatc aggctcagaa attaaaaaga gcaccgggaa agcatttccc aacccgtttt
     6301 tttgtggtat agtaaagtat aagtttggta gaagattgga agagcagagc atgaaaaaac
     6361 tgacgataaa tgaaattgcg gaaaaagcag gcgtgtccaa gacaaccgtg tctttttacc
     6421 tcaatggaaa agccaataag atgtctgagg aaacccagca gcggattcag cagattattg
     6481 atgaaacagg gtatgagccc agcgcggcgg ccagagccat gaaggccaaa agcagcggtc
     6541 tcattggcgt gatcttaaag gatgcggcag atccctactg tgccagagcc ttaaagggaa
     6601 tgggagaggc agccggtgag acggagtatc agattcttat tggaagcagc ggctatacct
     6661 ttaaaaacga gcgggagcat gtggaacgga tgttaaaagc aggggcagag ggatttatca
     6721 ttcaggccac ctaccggttt ggaatgctgg ctggcgaact ggaaaaaaag cgcaagccca
     6781 ttatctatct ggaggccaag ccctatgatt ttaagggcag gtatgtgaag ggaaataatt
     6841 atgactgcgt ctatcaggtg attacagaat gtatcaaaaa gggatatgga gactttctga
     6901 tgatttctga tggtgagagt tctatgggaa ctgccttcga gaatacagcc ggttacagag
     6961 atgccttaca ggatgcagga ctggagggga gaaccctgta cgttcagtcc ggcgcacggt
     7021 cggctcagat ttttgaggcc ttaaaggatg aactggattt aagtctccat accctgatct
     7081 atgtagcggc tccagaactt ttaaaggctg tttttcaggc gctgcgttcc cttccggatt
     7141 atgaacgcct ttttcctgag catctggggc tcattggctt tgaccccgaa ggctggacgc
     7201 ggatgaccag cccgccggtt tccgctatta tccccccggc gcaggaagag ggaaaacgtg
     7261 ccatgacgga gcttttagag ctgctggacg gaaaaaagaa ggatacagag gttgtgttta
     7321 agaatattgt gaaatggaga gggactacgg tctgagcgct ataaaaatga gggtatcgcc
     7381 caatcattat caaatcagat gattgggcgg taccctcgtt ttatacattt atacatgaaa
     7441 aaattcagag aaactctgtt ggcagccagc ttatcctgca gggaattaca gagtaccttt
     7501 ctgaaggatg atacatccgt agagtgcagt ttcaccagta gctactacag cataagcatg
     7561 ttctgcagcc ttatagaaat caaaacgttc aatcataccg atacagtcat caccacgggg
     7621 gtcatggcgc cggacgatct ggcggtattc ttcccaaatg ggggtgggta tgtctttgtc
     7681 acattcctgt ttgtccatga tcagaactgg agcttttaca gaagcgtcca gagggaaaaa
     7741 atgcagaatc gcgtctaaaa gttctgtagc ccgtactcca tcagctctca ccagcatact
     7801 gtggcgggcg ctggaggcgg agggataaaa cgcgtctcca atgaccagag tatctccatg
     7861 acccatttct gcaaggactt ttaaaagatc aggggaaaga cagtttggaa ttcctcttaa
     7921 cataatgtgt acctccgtaa tcgttatttt aactgccggt atcatttgca ggcagcagtt
     7981 tgatgataaa agtggttatt tctgtctctg actacaaaat agcacagctg ttaaaaaaaa
     8041 gcaaaaacaa attatgaaac agaatgaaac ggaatggaaa aacggctgat tttcaacaca
     8101 atatccaaca agatagataa aagaatgtga aaagtgtaca ttttagtatc atgacgttat
     8161 gattttttta caatagtttt tccaaaatct gatagtaaat tttacatttc tttttttata
     8221 cttatattca tccgatccgg tgggctgaaa tctaaaaaag ggtatatctt tgtaatgttt
     8281 attttaacag ccggcatggt ttgcaagcgg cagtcaaatg ataaaaggca aaggaagtta
     8341 tgaaacagaa tgaaacggaa taaaaaacag ctgattttcg acacaatatc caacaagaca
     8401 gataaaagaa tgtgaaaagt gtacatttac tttgtgcaaa atgtgtgatt atatagaaat
     8461 ataaagaaga aatagaacga aatgaccaaa aataggcgga tgaaacagaa cccgcagaaa
     8521 gggaagcgga tggccgtaaa cgtaagagaa attgcaaaaa aagccaatgt ttctgctgca
     8581 actgtttcaa gagtgttaca tcaatcggag caggtaaagc cggaaaccag aaaaaaagtg
     8641 gaagaagcga tgagacagct tcaggtaaca gcaggggaac tggtacgcag tgccaggatg
     8701 gataccagaa ttgtgggaat tctggtgcca gatctgtcca attccttttt ttcagagtgc
     8761 attttgggaa tcgaagaata tgccagggaa gcgggactga ccaccattat ttgccatacg
     8821 aaggagtccg accaggaaga agtgaggtat cttcggatgt taaaggaaat gcatgtatgc
     8881 gggatcatta tgactcccac atccgatgat aatgactata tgaacaatga gtatttaaat
     8941 cttttgatcg acatgaaaat tccggtagtt ttactggaca gggatgtgaa atactctcag
     9001 ctggacggtg tatttattga taatgaaaat ggggccttta atgcagtgaa gcttctttta
     9061 gaaaacgggc atcgcaatat tgccatgatt tctggccctt taaatacggt gccggggcgt
     9121 ggaagagaag aaggctaccg caatgcattt gaatttatga atgtaccctt aagggaggaa
     9181 ttgatctatg aggggcattt tagtattcag ggaggcagag aggccacgga aatgatatta
     9241 agggaacatc cggaggtaac ggctattttt tcttctaaca accatatgac gatcggctgt
     9301 ctttcccgcc tttttgagga agggcttaaa ataccggagg atatggcggt gataggattc
     9361 gatgacattc cgatcctgaa tcagcttggc tatgaaatta cggtggtagg caggcccacc
     9421 aaagagatgg gacatcaggc tgttaaaatt ctggctaaaa tgatgaacgg aaaacgcact
     9481 gatatgacgc ggagaatcat tctgattccc aagctaatta tccgcggttc ggaacggctg
     9541 tgcaggccgg cagattaaac agggaaagga gaatgacagg ttatgaaaaa agtggcggtg
     9601 attggaagct ttattgtgga tcttatggcc aggacgcctc atcttccaga gccgggagaa
     9661 acggtgatgg gaagcttttt taaggctgga ccgggaggta aaggagggaa tcaggcagtg
     9721 gctgccaagc gggcaggaag caatctggta tactccacaa agattggtaa ggacagcttt
     9781 gcagagattg ccagggcctc ctttgcggaa aacgggattt ccatggatta tgtgtttgag
     9841 acggagaagg ctgccacagg agcggcactg atttctgtgg atgaagttac ctcccagaat
     9901 gaaattatcg tgattttagg agccagcgcc acttatgagg aggaggatct ggaaaagctt
     9961 aaggaggcac tttcaggctg tgaatacctg cttcttcaga tggagatcaa tcgggaggct
    10021 acagaaagat tgatttccat ggcctacgat atggggattc gtgtgatttt aaatccggcg
    10081 ccggtacagc agctttcaga agagttatat tctaagctgt atctggtaac gcctaatgag
    10141 gtggaagcca gaatccttac agaaattgcc tgcgatgaca tagaagggtg caggcaggcg
    10201 gccagggcat ttatggaaaa aggcgtggag cgggtgatca tcaccatcgg caagcggggc
    10261 gtgtattatt atgacggaga gaccgaggaa atcattggaa attataatgt gaaagtgctt
    10321 gataccaccg gagcaggaga tgcgtttaac ggaggacttt tagcagggct cagtcaggga
    10381 atggagcttc tttctgcagc tcgatatgga aacgtggtat cgaatcttgc ggtgacgaag
    10441 ctgggaacgg ctcctgctat gccaacaaag caagaaatag acgtttttat tgaaaaagaa
    10501 cagattaaat tttaaggagg ggaatagagc atgagttcaa tcacattgaa gaacattcat
    10561 aaggcttacg gaaaggacgt gatcattccg gatcttaatt ttaccattga agacggttcc
    10621 tttactgtat tggtaggacc ttccggctgc ggaaaatcaa ccaccttacg gatgatcgcg
    10681 ggactggaaa aacccacatc aggagaaatt tttattgatg atgaaaacgt agtggatgta
    10741 gatccgggca agcggggcat cgctatggta ttccaaaatt atgcattata ccccaccatg
    10801 tccgtgcggg acaacatcga atttggattg aagaataaaa aagtgccgaa ggaagagaga
    10861 aataaactga ttgccgatgt gagcgaggtt gttggcctga cgccgtatct gagcagaaag
    10921 ccatctgaac tgtccggcgg acagagacag cgtgtggcat tggccagagc catggtaaaa
    10981 aatcccaagg tatttttgat ggacgagcct ctgtccaacc tggatgccaa gctgcgtcag
    11041 cagatgcgtg tggagctgat tgaactgcac aagcgtttgg gaactacatt catttatgtg
    11101 acccatgatc aggttgaggc tatgagtatg ggagataata ttatccttat ggataagggc
    11161 attattatgc agcgctctac gcctaaggaa atgtatcaca atccccagaa tgtctacaca
    11221 gcccagttta ttggtacgcc gcccatgaat attgtgagca atatggtgaa agggatccag
    11281 gtgggattta ggccggaaaa gacttccctc attgatccgg gagacgctgt cctttccatt
    11341 cagggacgga tcattaccaa ggaacagctg ggagctgaga tgaactattc gattgaaacg
    11401 gcctttggaa aggtgatggt aaagacggag gatgatattg aaggtcaaga atgccgtctg
    11461 tacatccggg aaaagaatgt ctttgccttt ggggaggacg gaaaccgcat tcctgtgaca
    11521 gaggagatta aaaaggcttt ggaacaggtg gagaaagggg tgtagagtat ggaggataaa
    11581 aaaggttctg taaaacagaa ggggaacttt tggaatcatg accgcaagga tgccttaaaa
    11641 ggctatcttc tgatttttcc atccattctg cttctgggga tttttacgat ttaccctatt
    11701 ttttacctgc tggactccag ccttcgggat ggaagccttt taagtaaaaa gagggagttt
    11761 gtaggcctgg aaaactttat cactctgttc aaaagtgtgg attttcacac gactctgaaa
    11821 aatacgttta tttattccgt tggtctggtg gcaatcctta tggtgctggc tactttgctt
    11881 gctgtatggg tgaatggaaa agctcagaag cgtttaaaca gtgtgacatt agcagcagtg
    11941 tttacccctc atattatttc tctggtttct gtatctacgg tatttttatg gatcatggat
    12001 ccccagattg gagcaatcaa ttatgtgttg cgcacactgg gcttaaagcc cttcccattt
    12061 ttagccagca gtaaaactgc attggcatct ctggtaggaa tgatggtgtg gaaaagcctg
    12121 ggatattatt cactcttaat tctggcggct ctgcagggag taccaaagga catttatgag
    12181 gctgccgaga ttgatgatac acccaaaatc cgtacattct ttcggattac cctgcctatg
    12241 atcagtccta ctctgttttt cactacgatt gtggctacca tcagttcctt tcaggtgttt
    12301 gagacggtaa atctgatgac tcagggaggc ccggtgaact ccaccaacac gttggtatac
    12361 cagatctatt cggatgcttt taagtactta aagctgggac cggcgacggc agaggcagta
    12421 gtgcttttga ctatggtagg aattttaacc ttcctgtatt tcttcttcct gggcaagaag
    12481 gtacattatc aataaaaagg aggctgcgac gtgaaaaaga cagttatgaa aaaaaaggga
    12541 cgcattctgg gaggagcgtt gaatgcggca ttaaagcttc ttttaatctt tctgatcgcg
    12601 tatcctttta tctggatggt atttacatct tttaaaccgt ataaggagac aacgatttat
    12661 ccgcctaccc tgctccctac caactggaca acagaagggt atgtaaaggc gttggaaatg
    12721 gtggatgtgg caacgtttct taagaattcc ctgattgtaa gcctgactgt tctggtactt
    12781 cagtatctgg tgattgtacc ggcggcttat gcctttgcca gatgtaaatt ccgttttaag
    12841 ggatttttct ttggtatcgt actgcttggt tttatgattc ctcagcaggt tacctttatt
    12901 cctatttatc tgatgttcag caaggcaggg ctgctccaga gcctgattcc ccaaattctt
    12961 ccgtttatct ccaacgcgtt tggaatcttc ctgctccggc agtattttat gcagatcccg
    13021 gaggagatta ttgaggcggc cagactggat gatgcgggag agttaaaaat cattttgagg
    13081 attatgatcc ccatggcaag gccggctatt tttacgattg gacttctcag ctttatttcc
    13141 agctggaatt cctatttctg gccgcttatc atgacgacta aggatgctta ccgcacactg
    13201 ccgatcggtg tggctatgtt gaacagccag gagggcggaa ggctctggaa tgtcctgatg
    13261 gctggaaata tgtttctggt ggttccgatc ctgatcgttt atctgtttgc aaataaacag
    13321 attcgcaagg catttgctta ttctggaatt aaataaaaac ggaggctgca catgaaaaag
    13381 aaattagttg tactgtcggt ggactcgctg tttgacgagg acatggaatt tttaaaaact
    13441 cttcctaatt ttaagaagat actggatcaa ggctgttacg cggaaggcgg gatgaggagt
    13501 gtctatcctt ctttcacata tcccgcccat gcatccatta tcacgggaac ctggccggag
    13561 ttccatggaa tttaccataa cgagaagtta gatgtgggaa atccttcgcc ggattggtat
    13621 tggtaccata aggatctaaa ggtggatacc attttggatg tggctcaccg gcaggggctt
    13681 acgactgcgt gcgtgggctg gccctgtatg ggagcagatc ccaatgtgga ctggctggtg
    13741 gcagagattt ggcctgagaa tgataaagtg gatcccagac ctattttaaa gacaggatgc
    13801 agtgaaaaca tgtttgaggc ggggggagtt atggagcgcc attggcacaa gctgagaaaa
    13861 accacccagc cctttatgga tcagatgatg gtaggtgcat cctgcgatat catacgccgt
    13921 tatcagccgg atatactgtt tattcatctg gcccatctgg atcatacccg acatgccaat
    13981 gggattcatg ggccggctgt gaaccaggcg atcattgcca atgatgactg gcttggccgc
    14041 ctgatggagg ctgctatgga tgcaggggtt tatgaagata caaattttgc agtgatcagc
    14101 gatcatggcc atttgccagt gaagcagatg ttcaatccca atgttctttt agcagaagaa
    14161 ggcttcatcc ggttggatgg aaatggaagg attgccagct gggatgctta ttgtaattca
    14221 acctcgctgt cctgtcaggt ggtgttaaaa gatccggcag acaaagaggt gagggataag
    14281 ctggaagcgc tgctgtacca gatgaggaag aatcctgcct atggtgtgga atgtgtatat
    14341 aataaggaag aggtggagaa agagcagcat cttaccggag attttgagta tgtgctggaa
    14401 ggaatggcag ggacaagctt tggagctgcc tgcaccgggg aagttttagt gaagccggat
    14461 aattccgatt ataaattctc ggtagcgtct cacggccatc tgccccacaa gggcccacag
    14521 cctgtattta tgatagccgg accggacgtg aagaaagggg cagtgatccc cagacagcgg
    14581 atcatcgacg aggcacctac gtttgcaaag attttcgggt tcgatctgcc agatgcaaaa
    14641 ggtacggttt taaaagaaat tttaaaataa attcctacaa ggaggagaaa ggaaggtacg
    14701 ttttatgaaa agaagaggag ctttggtaat gagcgcattg ctgatgacct cgctgctgtc
    14761 cggatgcgga ggcggccagg cggacgctga aaagactcag gaggcggcgc agacgcaagc
    14821 tcagggagaa aaccagtctc aggcagaggc tggggagaca gctgctgtga atcccgatgc
    14881 tacggaagtt ctgttctgga tcagtttaag cggcggtatg ggtgagacga ttgaagagat
    14941 tgtaaataat tataatgcca gccaggacaa ggttcatgtg acggtagaat tccagggaaa
    15001 ttattatgat atggcagcca agctgcagac agcagttact tccggcgaga tgcctcatgt
    15061 agcacagctg gaaatgggcc gtacaaaaat gtttgcagat tatggcgttc ttcaggatat
    15121 gacagaactg gctcaggagg cagggctgga taccagtatg ttttttgaag gccttatgag
    15181 cagctgtgac tggggagagg gattatatgc gcttcccttt aaccgcagca ctcctatgtt
    15241 ctactacaat aaagatatgt ttgaagaagt gggattggat ccggaagctc cgcctcagac
    15301 ctgggatgaa ctgaaagaat atgctgcaaa gctttctatt cccgacgagc gatggggatt
    15361 tgagatgccc attgacgcct ggttctacga agcatttatt atgcagtcgg atggacggat
    15421 tttgaatgag gatgagacag acataggctt taacaacgag acgggaacag aacctctggc
    15481 tatgtggaag agcatgatca gtgaaggcat catgaaggcg cctccaggaa aagagtacaa
    15541 ttcctatgaa gcagcccgct cagatttctc cgcaggtatc actggcatga ttgtgacatc
    15601 ttccggtgat ctgggtactc tgaacagctc ctgtgatttt gaagtaggga ctgcattcct
    15661 tcctaaaaat acccggtttg gtgttcctac cggaggagcg aatgtggtaa tgatggctgg
    15721 tttggaagaa gattcggcag ctaccatgga tttcctgaaa tacttaacca gccctgaggt
    15781 tgcagcatat tgggctacaa agactggtta tgtaccttcc agccaggctg cagcagatac
    15841 agatgtatat caggagtata tcagtgctaa tggcaatgcc aagactgccc tggagcagat
    15901 gcagtacacg gatattccaa gacccatcaa tgaggattat ccacagatcc actctgagat
    15961 catgatgaca gagattcagc gctgcattga ggagccttct tatacaccgg agcaggctgt
    16021 aacagcaatc tccgagcaga cgaaagctct tttagctaaa taaacaaagc aagtatcata
    16081 acgtatggaa gcacaggaat caaacctgtg cttcctgcat caaagggaaa atgcataaac
    16141 atggaggaaa aaatggaatt gaacaagtat attgaccata catatttaaa gccttttgca
    16201 atggaatcgg atattatcag aatctgtgac gaagcaaaga aatatcattt cgcgtcggtg
    16261 tgcgtgaacc cctgtaatgt tccgctggta gcaaaggagc tggcaggaag cgatgtggct
    16321 gtctgcagcg tagtaggctt cccttttggt actcatacta ctaagacgaa gctgtttgag
    16381 accagagaag ctctggacaa tggagcagat gaaatcgata tggtaatcaa tgtaggacgt
    16441 ctgatccagg gagatgaggc gtacgtggaa caggaggtag ccgctttggt acagctgtgc
    16501 catgagagag gaaaaattct caaggttatt attgaaacct gctatctcaa tgaggagcag
    16561 attaaagcca tgtgccgcat tgtggaaagt acccatgcgg attttattaa aacatccaca
    16621 ggctatggtt ccagaggtgc aaattttgaa gatattgagc tgtttaagaa atacttgaag
    16681 ataccggcaa agatgaaggc ctccggcggt atccgtacta cggaagatgc gaaacgatac
    16741 gtggaatcag ggtgcagccg tcttggaacg agcaacggtg tgtccattat ggaaggaacg
    16801 aaagcacagg aagcatatta aagaatccgg gggattattt gtgttgagat agcggttttg
    16861 aagctgcggg aaagggagaa ggaggagaag attgagatgg tgagggatat gatcgccatg
    16921 ctttccgatg tgaatctgtt ttcaacggtc atgcgccttg ggctggctct tcttctgggg
    16981 ggagttttag gcattgaacg tgggagaagg agacgtcctg ccggtctgcg cacatacatg
    17041 atcgtgtgta tggccagtgc attggtgatg cttaccggcc attttctgtt tgaaacattt
    17101 catacaggag atccttcccg actgggagcc caggtcataa gcggcatcgg ttttctcggc
    17161 gctggaacca tcattatcac ttccaaacag gtgaagggac ttacaacggc tgccggtctg
    17221 tgggcttctg cctgtctggg gctggcagtg ggagccggtt tttatgccgg agctatcaca
    17281 tgcggaattt ttttgctttt agtattttcg tgtatgtcaa agctggatac ccgtcttcgc
    17341 aaaaatgcaa agcaaatcag tttttttgca gaatttaagt ccatgaaagc tctgggagaa
    17401 tttatccata tgatgaggga taaaaattac aagatttatg atgtagagat caatcggagc
    17461 aaggatacag tgggagatct ggtgggagct gtattctggg tggatttaaa tgctcctgcc
    17521 gatcatgttc aggtcattgc agatttttcc agttttcagg aggtcaggta tgttgaagaa
    17581 ctggaggtat tgtagagccg ggctttaagc tgttgttttt ctaaaaagga gatgaaacgt
    17641 ttcatatgta tggcgttcat ctcctttttt gatacagcgg ataagcggcc taacaaaata
    17701 tgaaataaaa atggcggagt gcttatcgta acactccgtc gaaagtctcg ccgggttgcg
    17761 catgaaaaat cagaggcgtg gtgggttctg ttgctatcat tatacaacgt ttattgcggc
    17821 tgtcaagatt cattttcttt ttgagtttcc gcaaactgta actgtaatta attgtaactt
    17881 aattgtttca aacattctgt taaaatcttt ctaatatgag gaattcaatg aaacaggggc
    17941 attgcaaaac ggaaagcttc cataggaact tcgttttaca atgcccttct ctttgtttta
    18001 taaaatattt agttactatt cttctctgcc ttcatctcag ccatcttcat tgcacgaacc
    18061 ttcaatggca ggccgaacag ggtgataaat ccgtctgcat catggtggtc atagaggtcg
    18121 ccggtggtga aggaagccag ggactcgctg tataaggagt aaggagaggt ggttccggcc
    18181 ttgatgatgt tgcccttgta gagcttgaac ttaacctcgc cggttacata ttcctgagtg
    18241 gactctacga atgcctgaac agcctcgcgc agaggagtaa accattttcc ttcatatacg
    18301 atctgagcca tacggttgct catgtccttc tttacatcca tagtagcgcg gtccagaacc
    18361 agctcctcta actgcttgtg agcctccatc aggatggttc caccgggagt ctcataaacg
    18421 cctctggact tcattcctac tacacggttt tctacaatat ctacaatacc gataccatgc
    18481 tttccgccca gctcatttaa cttgcggatc acatcggaaa ccttcatctg tacgccgttt
    18541 acgctggtgg gaacgccctt ttcaaaggtc atggttacat attcgccttg atcgggagct
    18601 tcctcaggag taacgccaag taccagcata tttgcaaagt ttggctctaa ggaaggatct
    18661 tcaagctcca gtccctcatg actgatgtgc cagatattgc agtcgcggct gtagctgtgg
    18721 ctgtgatcga agggaagatc gatgccgtgc tgcttgcagt attcaatctc atcctcacgg
    18781 gactgcattt tccatacatc ggtcatacgc cagggagcga tgatcttgat gtcgggagcc
    18841 agagccttga tggataattc aaaacggatc tggtcatttc ccttgccggt agcaccgtgg
    18901 cagatggcaa ctgcaccttc cttgcgggcg atgtcaacca ggcgttttgc gatggcagga
    18961 cgggccatag aggtacctaa caggtattta ttctcataaa cagcatctgc ctttacgcag
    19021 ggcataatgt agttatcaca gaaatcatct accaggtctt caatatataa tttggaagcg
    19081 ccggaaagct ttgctctctc ttccagacct tccaactctt tgccctgtcc gcagtcgatg
    19141 cagcagcaga ttacgtcata tccaaaattt tcctttaacc acggaataac agcagtggtg
    19201 tccagaccgc cggaataagc taaaacaact ttttctttca tcgttatatc ctccaaaaat
    19261 catattcaac aatctttcat aaatatacac cacaattcgg gaaaatgcaa gtataaaata
    19321 aaaaaataaa aaaatgttgc atattatgca tcttagatgt ataattatga aatcaaaact
    19381 ccagagggag agattcaggg aaaaagaacg gctcattttc cctcaaaaaa tctcaattag
    19441 cgggatgttt aaatatgaat ggtttcttat ataaaggaga gtatgaatga tgattaaggc
    19501 aggaattatc ggatccacag gatatgcagg aggagagctg gcaaggcttt tgcttcagag
    19561 agaagatgtg gagatcaaat ggtacggttc cagaagctac attgaccaga aatatgcatc
    19621 tgtttatcag aatatgttta aactggtgga tgcagagtgt atggatgaca atatgaagga
    19681 actggcagag caggtggatg tggtatttac cgctactccc cagggcttat gctccactct
    19741 ggtaaatgag gatgttcttt ccaaagtaaa gatcattgac ctcagcgcag atttccgtat
    19801 taaggatgtg gaaatttata aacagtggta taagctggag catggttcac cggaatatat
    19861 taaggaagct gtttacggac tgccggagat taaccgggac aagattaagg atgcaaggct
    19921 gattgccaat ccgggctgct ttcctacctg ttccttttta tccacatatc ctttagtgaa
    19981 agaaggaatc attgatccca atacactgat cattgacgcc aagtccggca cctcaggggc
    20041 aggaagaggc gcaaaggtag acagcctgta ctgtgaggta aatgaaaata tcaaggcata
    20101 cggggtaggc gttcaccgcc atacgcctga gattgaggag cagctgtcct atgcggcagg
    20161 aaaacccgtt actatcagtt tcaccccgca cctggttccc atgaaccggg gcattctggt
    20221 aacagcctac gcatctcttg caaaagatgt aacggaagaa gagatcagag ccgcctatga
    20281 gaaatattat aagaatgaat attttgtccg cctcttagaa ccgggagtgg ttccccagac
    20341 aagatgggtt gagggaagca attttgcaga cgtaaacttc aagcttgatc ccagaactaa
    20401 gagggtcgtg atgatgggag ccattgacaa tatggtaaag ggcgcagccg gacaggcagt
    20461 ccagaatatg aacctgatgt ttggccttcc ggaaaacaca ggcttaaagc aggtgccgtt
    20521 attcccgtga tacaggggct gaaagccgtt tgacacatca tttttcaaaa gagacaggag
    20581 ggagcagtac caatgtcagg aacaatggag attctgatcc gtgaaatgac catagaggat
    20641 tatgaagcgg tatatcatct gtggacagag atccggggct ttggaatccg gagtatagat
    20701 gattcaaaag agggagtgga gcgatttctg aaacgcaatc ccaccaccag cgtagtggca
    20761 gttcagaatg gacggattgt gggaaatatt ctctgcggcc atgacgggcg cacgggctgc
    20821 ttctatcatg tctgtgtaga tccggattac cgcaagcacg ggattggtta ccggatggta
    20881 agagccgcca ttcacgccct gcagaaagag ggtgtcagca agatcagcct cattgccttt
    20941 aagagcaatg agattggaaa tgccttctgg cagggcattg gctggacgga acgggaagat
    21001 atcaacagct atgaatttat attgaatgag gaaaatatca cacggtttgt aaaataggag
    21061 gaaatgtatt atgtatgaag tgattcaggg cggcgttaca gccgcaaagg gatttatggc
    21121 agcatttacg gcagcaggaa ttaaatataa gaaccgtccc gatatggcta tgatctacag
    21181 ccaggttccc tgtgagtccg caggaacctt taccaccaat cttgtaaagg cggctcctgt
    21241 caaatgggat aaggcccagg tgacaagcgg cgcgaaagcc caggcagtga tcgtaaatgc
    21301 aggaatcgcc aatgcctgta cgggaaaaga gggaatggac tactgcgctc agactgccaa
    21361 ggctgcagca gaagcattaa atattaagga agacagcgtc cttgtggcct ccaccggagt
    21421 gatcggaatg cagctcccca tggaacgcat tgaagcaggg atccgggcca tggcccctga
    21481 attgggggat agcttagaga gcggaactga ggcttcaaag gctattatga caacggatac
    21541 gaagaataag gaagtggctg tcaaagtagt tcttgggggc gtacctgtta ccatcggcgg
    21601 catgtgtaag ggatccggca tgatccaccc caatatgtgc accatgttaa gctttgtcac
    21661 aacagacgca gccatttcca aggaacttct gcaggaggca ttaagcgagg atatcaagga
    21721 tacctacaac atgatttccg tagacgggga cacttctacc aatgatacgg tgcttcttct
    21781 ggcaaacggc cttgcaggaa atcctgagat cacagagaaa aatgaggatt acaaagcctt
    21841 ctgcgaggca ttaaattata tcaatgaaac acttgcaaag aagatggcag gagacggaga
    21901 aggctgtacg gctctttttg aggtaaagat caagggagcg cagacaaagg agcaggcaaa
    21961 gattctggca aaatccgtga tcacttccag ccttacaaag gcggcgattt tcggtcatga
    22021 cgccaactgg ggccgcatcc tctgcgccat gggctattcc ggcgcggagt ttgatccgga
    22081 gcaggtggac ttgtattttg agagcgctgc aggcagcatg cagatcataa aggatggcgt
    22141 ggctgtggac tacagcgagg agcaggccac aaagatcctt tccgaaccgg aggttacagc
    22201 catcgcagat attaagatgg gaaccgaaga ggcggcagcc tggggctgtg accttacctt
    22261 tgattatgta aagatcaacg cagactaccg gtcataagat ggcggcagga aacagggaat
    22321 atagaatatg aattcaggga ggaaatgaga aatgaatctg gaaccaatgc agaaggcggc
    22381 tgttttaatt gaggcccttc catatattca gcgttttaac cgcaaaattg tagtggtaaa
    22441 atacggcggc agcgccatgg tagatgaaga gttaaagaaa aaggtcattc aggacgtagt
    22501 tcttttaaaa ctggtaggct ttaagcccat tattgtccac ggcggaggaa aggaaatcag
    22561 ccgctgggtc ggaaaggtag gcatggaacc ctattttatc aacggcctgc gcgttaccga
    22621 cgagcctacc atggagattg cggaaatggt attaaataag gtaaataaga gcctggtaca
    22681 gctggtaaat gagcttggca tcaaggcagt gggaatcagc ggaaaagacg gcatgatgtt
    22741 aaaatgccat aaaaagcttt ccggcggtca ggatatcggc tatgtgggag aaatcgattc
    22801 cgtagatccg gaaattatcc atgacctttt ggaaaaggat ttcctgccga ttatctgccc
    22861 cattggctat gacgagcact tccagagcta caatatcaat gcagatgatg cagcctgcgc
    22921 catcgcaagg gcggttcagg cggaaaagct ggcctttctt acagatgtag aaggcgtata
    22981 taaagatttt gaagataagg attccctgat ttctgaaatg gaagtggatg aggcacaggc
    23041 ctttgtagac agcggaagcc tgggaggcgg tatgcttcca aagcttcaaa actgcattga
    23101 cgccattaaa aacggcgttt ccagagttca tattctggat ggacggattc cacactgcct
    23161 gctgcttgaa atctttacag ataagggtat cggaactgcc atctataatt caaaagagga
    23221 tgagaggtat taccatggct gatgaaaaaa tgatcaatcg ggctgaacag gttctgtata
    23281 aaacatacaa tcgcttcccg gtggtgtttg accacggaaa aggagtaact ctctgggaca
    23341 gcgaggggca ggaatatctg gacttcggcg caggaattgc ggttatgggt ctgggatacg
    23401 cagatgagga atacacccag gcggtaaagg cccagctgga taaattgacc catatttcca
    23461 acctgtttta caatgaacct tcaatcacag caggagagaa gctgttaaaa gtctccggca
    23521 tggacaaggt gttttttacc aacagcggca ccgaggcggt tgaaggagcg ttaaagatcg
    23581 caaagagata tcattataat aagcttcatg aaacaatggg ggacggcttt gacggctgcg
    23641 aggaaaagga aatcgacatg acaggagaga tcattgccat gaaccattcc tttcacggcc
    23701 gcagcctggg cgctttgtcc gtaacgggaa atgcccacta tcaggcgccc tttgctccgc
    23761 tgatcccggg aatccgtttt gcggatttta acgatctgga cagtgtgaaa tcacagatta
    23821 cagacaagac ctgcgccatt atcatggaaa ccatccaggg agagggagga atttacccgg
    23881 cagaggaagc ctttttaaag ggcgtccggg cgctgtgcga tgagcacgat atcctgctga
    23941 tcctggatga gatccagtgc ggaatgggac gcaccggaga gatgttcgcc tggcagcatt
    24001 acggcgtaaa gccagatgtg atgaccgtgg caaaggccct tggaaacggc gttcccatcg
    24061 gagccttcct ggcctgcgga aaggcagcca cagctatggt tccgggagac cacggcacca
    24121 cctacggtgg aaatcccttg gtgacagcgg cagcgggagc agttcttgat atttttgaaa
    24181 agagacagat cacagaccat gtcaaagagg tgggagctta cctttatgaa aaactggagg
    24241 agcttaccaa aaaatacgat atcatctgcg gacacagagg tcggggactg atccaggggc
    24301 tggagtttac cgttcctgca ggccctattg tatcaaaagc tcttttagag gagaagctgg
    24361 ttttgatcag tgcaggcagc catattatcc ggtttgtgcc gccgcttatt attcagaagt
    24421 cggatgtgga tgaaatggcg gcgcggctgg aacgggtgct gaaaaattta taaggtgtgt
    24481 aaaagattcg tatataagcg aaaagacaga gttgtataaa aataggggga taaatcaatc
    24541 atcaatgcca gatgattgat ttatccccct gattgctaaa gattgaaaat atccgcatgt
    24601 gttcctgtgt ccaccagagt gagggtcagg atgtcatttt cgataagata aactaaaagc
    24661 caatctggtt taatatgaca ttcacggaag ccttttaact agccgcttaa ttcgtgatca
    24721 tgatattttg gttccagttg tttaccctga cgaagtgtgt caacaacata atccaaaagg
    24781 gcaagatcca gtcccctttt tttcatgagt ttgtagcttt ttttgtaagc ggttgtgaat
    24841 tttactgtgt acatttattc ttcaagtgtc cttcgcagtt catacatact attatatccc
    24901 ttgacgtcag gatcacggga gatgcgtttg gcttcatcca tggcatcaag tgtgcgctga
    24961 ttgtaatggg gcatttcaat attaaatgga agtccgcctc taagcacaca ttgatgaaga
    25021 aacaaattta ctgcaccaga catatcgagt ccaagggtat taaacaaatc agtggcctgt
    25081 tttttgatgt ctgcatcaat tctgatctgg gtaggtgctg ttgccatata atcaccgcct
    25141 ttcaggtata ttatatgtaa tttggtttac aatgtcaatc aaatagatgg taaaatcact
    25201 ttttccttga ttttttagca tttttaaggc tcttttttga ggctttttta gccttggagc
    25261 ctttttttgt tttttccggt ttgtcggagc ggccaacgga cagaaaatca gaatcctgaa
    25321 gctctggagt ggtatctgtg atcggaggta cagtgtccgg ggcgggagat tgagggctgg
    25381 aagctggcgg atcagacggt atctgttcat ctgccctata ttccacgata tcctcaatac
    25441 ggcagtctaa aaggcggcag agtgtttcaa gcgtgtgagt agaaacgtgc atattttttt
    25501 tcaggcgtcc gatctgtccg gcgctgaagc catgaaattt gattagttta tactgggtca
    25561 taccgcggtt tgtcatggta tcccataaac ggtcatatcg aatcattgac cattcctcct
    25621 aaataatata ttatcagtaa acggattata tcataagatg gatatggata caagagggac
    25681 tgaaaaaata acgaatgaca aacctacagc acaatgatat aatttactga gcatcgtata
    25741 gtatgaggag attgtatgct ggattacagg atagcggcag tctgcggagc tgccctgggg
    25801 gctgctctgg cagcccgttc aaaatatgag agaaaatcgc tgtctgtgga ggaaacagag
    25861 gtgttttcgg atcgtcttac cagagactgg atctttgtat ttctttccga tctccatgaa
    25921 aatgaatttg ggccggaaaa tgaacagctg atagcagcca ttgaccgcat aaagccggat
    25981 gggattttaa ttggaggcga tctgatcgtc acgaaaaaga gacaggcaag aacaagagta
    26041 tcggagaccc ttctggccgc gctggcttcc cgttatccca tatactgcgg aaacggcaat
    26101 cacgaaagcc gcatgagatg gcggaaggaa gaatacaaag atggctatga acaatacaga
    26161 gcatatctta agaagctggg aatctgtctt ttagaagatc agtctgtatg gattggagac
    26221 gagatacggg tttccgggct ggaactggat ccctgttttt ataaaaaggc atattccaaa
    26281 aaactggcat tgatggatca agcttatata gagaaaaagc tggggcctgc cagcagggat
    26341 cattttcaga tccttctggc ccattcgccg ctcttttttg aggcatacag gaagtgggga
    26401 gccgatttga cgctgtccgg ccattttcat ggaggcacca tacggcttcc ggttctggga
    26461 ggcgtgatga cgccccagta tcagtttttt ctgccggaat gtgccggaac ccatgaaaag
    26521 ggcgggaaat atatggcagt aagcagaggc cttggaaccc attccatcaa tatccggttg
    26581 aataacaggc ctcagctttt attcgttcgg ttaaagagga aaagaacgta attatggaaa
    26641 caattaccta taaattggag cattttgaag gccctctgga tcttctgctc catctgatcg
    26701 aaaagaacaa gattgatatc tatgatattc ccattgtgga aattacggcc caatatctgg
    26761 cctatgtgcg ccagatggaa cgggaagact taaatatagt cagcgacttt cttgtcatgg
    26821 ctgcaaccct tctggatatc aaggccaaga tgcttcttcc gaaggaagta aatgaggaag
    26881 gggaggaaat agaccccagg gcagagcttg tgagccgtct gctggaatac aagcgttata
    26941 aacttttggc agaagaactg gcagagcggg aggagggagc gctgatgcat ttttataagg
    27001 cgtctaccct tccgccggag gttgcaaaat accagccgcc ggtagatctg gatgagcttt
    27061 tggatggaat gacgcttgca aagctgaaac ggatttttga gcaggtgatg aaacgccagg
    27121 aaaataaggt ggatcctatc cgcagtaaat ttggaacgat ccggagagag ccggtaagtc
    27181 ttgaaacaaa gataggttct gtactggctt atgcgagaaa aaaacggcgt ttcagttttc
    27241 gccagctttt agagcgccag tccgataagc tggagatcgt agtaaccttt ctggcagtgc
    27301 tggagctgat gaaaattgga aagatccatc tgacccagga ggaaatcttt ggagatatgg
    27361 atattgaagc tctggaaccg gagggcatgg aagaagaacc ggatcttgca gaactgagtt
    27421 attttgagga ataggaaatg gaaaacagca gtatcaatgt aaattttgag cctgtggaaa
    27481 atgaaaagga tcaggaggcc gttattgagg cagtgctttt taccataggc ggggccgtgg
    27541 agctgcgcca gctggcagcg gccattggac aggataagga aacggccaaa agggcggtag
    27601 agcggctggc aaagcgttac cgggaagaaa caaggggaat ggagattaaa cagttagagg
    27661 acagctatca gatgtgtacc cgctctgaat attatgaaaa cctcatccgc gtggcggcag
    27721 cgcctaaaaa gcaggtgctg tccgatgtgg ttctggaaac actgtccatt attgcttacc
    27781 ggcagccggt gacaaagatg gagattgaga agatccgcgg ggtaaagtcg gatcatgcgg
    27841 taaaccgtct ggtggagtat gatctggtct atgaggcggg ccgcctggat gcaccgggcc
    27901 gtcccgccct gtttgcaact acagaggaat ttatgcgccg gttcggtgtg gattctctgg
    27961 gagaacttcc ggatgtggat ccggaacagg aggctctcat ccgggcagag gtagaggagg
    28021 agctgcagct gagcgtggag gagcttcaga aagatatgga ggaatagccg gagaaaagaa
    28081 tcaggtgccg gaaaccgatg ccgcagtccg tcacctcagg ggacaaatgg gaatatgcta
    28141 gggtaacccg tttgaagagg agcggttttg tgtttcagag ttttattctg gtgctggcgc
    28201 tgtgtgccga cacctttggg gcaggttttg cctatgggac aaatcgaata cgggtcggtt
    28261 ttggaaaggt gctgttttta aatggaatct gcagcgtctg cctgggactg gctatgacaa
    28321 caggcttttt tgccgcagag aggctttcgg ggatcaatct tcgcactctc tgcggaggaa
    28381 gccttatttt tatgggattg tttcgtctca cggacttttc tctgcgaaga tggatccgga
    28441 tgcattggga gaatggacgc agtttctgct tttctcattc cggtattcat gtgcttatac
    28501 ggatctggag tgatcccatg gaagcagatg cagatggctc tcacagtctg gaatggaagg
    28561 aaacatttat tttaagcctt gcaatgtcct tagacagtct ggctgccgga gctgcagcag
    28621 ccctttcaga gggaagaatt gctcttacag tgcttttgtc tcttttggtg ggaactgcgg
    28681 tcacagaagc cgggcttatg gccggccgct ttgccgctga gaaagggcag agagacctgg
    28741 gatggctcag cggtctgctt ttgatagcgg tggggattgg gaagctgtag gggggcggaa
    28801 gctcttggta aaaatgtaca cataaaaaaa cttgcaaccc ctttgattat atgatacatt
    28861 actctggtaa agtgaagaag tgtatttgca atgcaaaggg aggacaggaa gatgctgcaa
    28921 tttttgaata ctgcgttaaa ggccattgat gatcttgtat ggggagttcc gctgattgtg
    28981 ctgattttgg caacagggat ttatctgacg gtacgtctga aggggatgca gatccgaaaa
    29041 cttcccgctg ctctgcgcta catggttcag aatgaagaag agggaagcgg agaggttacc
    29101 agctttggag ctctttgtac tgccatgtca gcaacgatcg gaacgggaaa tattgtaggt
    29161 gtagcgaccg ccattgtggc tggtggaccg ggcgctttat tctggatgtg gattgccgct
    29221 ttatttggaa tggcaaccaa atatgcagag ggtgttctgg caatctgcta tcgaagtgta
    29281 gacaaagaaa ccggtcatgt tttgggaggt cccttttact atatcgaaaa cggaatggga
    29341 aagaaatgga gatggctggg aaaaatattt gctttttttg gcgccggtgt cggtcttctg
    29401 ggaatcggaa cctttactca ggttaatggc attacttctg cagtaggaga tttttttgat
    29461 agtcagaaag cctgtacggt ggcgctgttt ggaagagagt atacggtggc cgtgctgatc
    29521 gccggactga ttttgacaat ttgtgttgca ctggtggttt tgggtggatt ggagcgtatt
    29581 gctactgttt cccagattat cgtgccgttt atggctgtct tttatgtggc tgcatgcatt
    29641 acgattttat gtgtaaatgc acaaagggtt cctatggcat ttaagatgat actggaaagc
    29701 gcctttgggc ttcggccggc agcaggaggc gctttgggaa gcattcttct tgcaatgcag
    29761 aagggaatcg caagaggcat tttttcaaat gaggcaggat tgggaagcgc tcccattgct
    29821 gctgcagcag cccgtaccag agaaccggtt cgtcagggac tggtttcgat gacgggaacc
    29881 tttatagata cgattataat ctgtaccatg acaggtcttt caattgtgct gaccggagcc
    29941 tatgagacgg gtctggaggg ggttgcagta accacttatg cctttaatca cgggcttcct
    30001 ctgcccgcct gggccagttc ctttcttctg atggcgtgcc tgattttctt tgcatttaca
    30061 actattatag gatggaatta ttatggggaa cgctgtctgg agtatttgtc aggaggaagc
    30121 agacgtgcag taatgacgta tcgtttctta tatatccttg ctatttttat cgggccgttt
    30181 atgacggttg aggcggtctg gacgattgcc gatatcttta atgcgctgat ggcaattccc
    30241 aacctgattg cggtacttgc cctcagcgga gttgttgcag cagaaacggg agattactgg
    30301 aaacgaatga aaaaataaga tagaagtctg attttaatac gcctattgac ggagccttgt
    30361 atccatggta agatagcatc accaattaag acacaataca tagtgtgctg acaaaaaaga
    30421 taaaacacaa tatatagtat aaaatataat cgttctattg agaggtgacg tatggatcta
    30481 accagagtaa aggtagtaaa aaaagacgga acaaaagagg attttaacgt tcagaaggtg
    30541 atttccgcca tcagcaagtc cgctgagagg gccatgatca cattttcaaa ggcagaactg
    30601 aattttatct gcgaatttgt agaagagaag gttcaggagc tggatcagga gctgatccca
    30661 attccccaga tgcacaatat tgtagaaggc gctcttgaaa aggtaaatcc tctggtggca
    30721 aagagctatc gggattacag aaactataag caggattttg tccggatgct ggatgatgtg
    30781 tataagaaga gccagtctat tatgtacatc ggtgataagg agaacagcaa tacggacagc
    30841 gccctggtat ctacgaagcg cagcctgatt tttaatgaat taaataaatc cctgtatcag
    30901 aagtttttta tgaccgtgga ggagcttcag gcctgccgag aggggtatat ttacatccat
    30961 gatatgtctg ccagacgcga caccatgaac tgctgtctgt ttgatgtaaa ggcggttctg
    31021 accgggggct ttgagatggg aaatctctgg tataacgagc cgaaaacgtt ggatgtggcc
    31081 tttgatgtga tcggggatat tgtcttaagt gcggccagcc agcagtacgg aggttttacg
    31141 gttcccagcg tggaggagat cctggcgccc tatgcggaaa agtcttatga aaagtacatt
    31201 gaaaaatatc tggatctggg tcttgcagcg gacaaggcca aagaggtggc ctggaaggat
    31261 gtacagaggg atatggagca gggctttcag ggatgggagt acaagtttaa ctccgtttct
    31321 tccagccggg gagattaccc ctttatcacc atgacagcag gcacgggaac gagccgtttt
    31381 gccaagatgg cgactatgac catgctcaat gtgcgcaagg gcggacaggg aaagaaggat
    31441 cataaaaagc ctgtgctttt cccgaagatc gtattccttt atgatgaggc tcttcacggg
    31501 ccgggaggcg agctggagga tgtgtttgag gcagggatcg actgctccag caagaccatg
    31561 tatcccgact ggctgtccct gacgggaaag gggtatatcg ccagtatgta taagcgctat
    31621 ggcaaaatca tttcccctat gggctgccgt gcctttttat ccccctggta tgagcggggc
    31681 ggaatgtatc cggcagatga taaggactct cccgtatttg tgggacggtt taatatcggc
    31741 gtagtcagcc ttcaccttcc tatgatcctt gcaaaggcaa gacaggagag ccgggatttc
    31801 tacgaggtgc tggactacta tctggagctg atccgcaaga tccatatccg cacctacgct
    31861 tacctgggcg agctgaaggc atctaccaat ccgctggcct actgtgaagg cggtttttac
    31921 ggcggtcatc tgggtctgca tgataagatc aagccccttc ttaaaactgc cacagcatcc
    31981 tttggcatca cagcctttaa tgagcttcag cagctgtata acaaaaagtc tctggtagaa
    32041 gatggacagt ttgcgctgga ggttctggag catatcaata aaaagattga ggaatttaag
    32101 gaagaggatg gcaatctgta tgccatttat gcgactccgg cggaaaatct ctgcggcctt
    32161 caggtaaagc agttccgtga aaagtacggc atcattgaaa atgtgtcgga cagggagtat
    32221 gtgagcaacg gcttccattg tcatgtgaca gaggacatta cgccgattga aaagcaggat
    32281 ctggagtacc gtttctggga actgagcaac ggaggaaaga tccagtatgt gaaatacccc
    32341 atcagctaca ataaagaagc catccgtact ctggtgcgcc gggcgatgaa gatgggcttt
    32401 tatgagggag taaatctgtc gctggcttac tgtgacgact gcggccatga ggaactggag
    32461 atggatgtgt gtcctgtctg cggcagccgc aacctgacaa agattgaccg tatgaacggt
    32521 tacctgagtt attcgagagt aaagggagat acccgtttga atgaagccaa gatggctgaa
    32581 atagcagaga gaaagagcat gtaagagaaa tgagcaaaaa ggccgtcggt atctgatgcg
    32641 ttcatcagac cggcggcctt ttatggctca gactgggatt tagccagtaa cccttctata
    32701 tatccttctg cctttttctt atctcccgga cttaaccggt caaacagttc cagaaaagcc
    32761 ttttgctgct ctgtgattgt agcggcggag ccgtccgtac tgtaaaactg ggaaatggta
    32821 atgcccatgg cctcgcagat atggcgtatg gtggggacac tggggatgga agtgcggcgg
    32881 aaaagatttc ctatgctgtt tggagaaacg tccatttcct ttgcaaggcg gtaatggctc
    32941 cagcctctga tgcggcagag ttcttcaata cgttccagct caaaactcat tgtagtcata
    33001 tcagaagaga catccttaga ggatcatctg cttctgcctc ctgtcgtgct gtcattttcg
    33061 tgctttgtat tattgtatcc cacagttata aaaaacatca ggctgaaata gagtgcatga
    33121 tttatgcacg aaatagtgta caaatgaata aaaattacct tatgcgcaga aatatacagt
    33181 tagaatctgc ggatgaaagg atctttaagc gcttcttctt tgggagagtc ggggcggtag
    33241 ccaaggaggc tgtttgggtc tgtttcaaag aattcacaca ggcctttcag ttcaatgtca
    33301 gtcaccagac gtttgccgag ctcgatccgc agaatggtca gcttggaaaa attgtagcca
    33361 ttgacctgca gtctggcggc cagttcatcc tgagtcagct ttcgagccat ccgcagctgg
    33421 cggatccggc tgccaatgat atttttctgc tcactggtgg ttatctttcg cataaaaact
    33481 cctgaattaa tggtgtacac aaggcacacc tgtaaaataa tggttatgta ctaactgatt
    33541 gtaatgaaga aaatcgaaaa aaaggttcca ataatgaaat ttgggaaaat aaattttgtg
    33601 gaataggggt cgccccctcc tccgtccgtt tcccagggct ccggtttccg gctgtaagta
    33661 aatctaagag gctgaaattt ttctaaaaac aaacggcata tcaaaaactc gctgcgctca
    33721 gacaggcttg atatgccgtt taacgaaaaa tttgatcctc taagatttac taacagcctt
    33781 caaagtcgcc ctgggaaacg gatggaggag ggggcttggg atactgtgag gaaggaagta
    33841 tgcacttcca tgaatttccc tgagggaaaa tcaggtggaa aaaaaagcgg gtttatgtta
    33901 gactggtagg tagtacaaaa gaaaggctgg cgtatatgaa aaagtttatt gaagagttta
    33961 aggcatttgc actgaagggc aatatggtcg atctggcagt tggtatgatt atcggcagtg
    34021 cgtttaccag tattgttaat tcgctggtga atgatatttt tatgcctgtg ctgagtattt
    34081 ttacgggaaa aatcgatttt tccaatatgt ttattgctct ggatgggaat aaatatccta
    34141 ccttggaggc ggctaaagaa gtgacggcta ccattaacta tggttctttt attactcaga
    34201 ccatcaattt tctgttaatg gctctggtgg tatttctggc agtaaaggcc atcagtcgtc
    34261 tggaaaaaat aggaaaacag ccggaagcgc ctaaagctcc tacggagaaa aaatgcccct
    34321 actgcagcac catgatcccg ttgagtgctg tgcggtgccc tcactgcacc tctaatctgg
    34381 atgaacagga aaaataaagc aggagaataa ataagatgcg gtatcacaat attacaaagg
    34441 atgatatgct gaacggagaa gggctgcgga ctgtgctgtg ggtggcagga tgcagccata
    34501 actgtcccgg ctgccacaat cccgtgacct gggatgtgag gggcggactg ccgtttgatg
    34561 aggcggcaaa agaggagctg tttgctgaac tggagaagga ttatatttca ggtgtgacct
    34621 tcagcggagg agatcccctt catccggcca acagagagga catagcccgt ctggcagagg
    34681 agatccgcgg gcgttatccc aataagacca tatggctgta tacaggctat gactgggagc
    34741 agatctgtga atttgcgctg cttaaaaata tagatgtagt ggtggatgga gaattcatag
    34801 aagcatataa ggacgtcagc cttcactgga agggaagctc caatcagaga gtgatcgatg
    34861 tgcagagatc ccttcttatg ggggaagcgg ttctgtatga ggagccggac acatgaggca
    34921 gagccctttg gcagaaaacc ggcgaaaagg gataaaataa gagattgaga acaaaataca
    34981 ggagaacaga aaaacgatga tcagccgtat tgcaaaattt cataaagtaa gctttgaaca
    35041 atttaaagga gactgggagg atacctttgg aaagcaggag gatatacgca gtatttatga
    35101 ggatatccgt cttccagagc gtgctacttc aggctctgca ggatacgatt tttttgctcc
    35161 aaaagcattt gtcattcagc cgggggaaac ggtaaagatc cctacaggca tccgggcaga
    35221 aattgatgag ggctgggtgc tggctttatt tccaagaagc agcctgggat ttaagtaccg
    35281 cctgcagctt aataatacgg taggtattat tgacagcgat tatttttact cagataatga
    35341 aggccatatg tttattaaga taacgaatga ttccaatgaa gataaaacgc tggaactgcc
    35401 aaagggcgca ggcgttgtcc aggggatttt cctgcctttc ggcatcacct tagatgacgc
    35461 cagcagcgga cagagaaacg gcggctttgg aagcacagat aaaggaagga actgatcatg
    35521 gaaggcagca ggagaagaag ctcctatcca ggggggagcc gtccgctggg agcaggccgt
    35581 cccggtgatg caggccggcg gccggtaagg gccggctctt catccggccg gagaaatggg
    35641 ggaaacccca ggcggagaag gagaaagcgc ctgatgaaac ggattctcac agcagcgttc
    35701 tgccttattc tcacaggagg aatcgccttt ggcgtaagcc gtctggcagg ctcctttcat
    35761 ttttcaaaga aaaagaagct cagagctcag ggtattgagc ttttaagggg aggagatgca
    35821 ggcggcgccg tttctgcgtt tgatgaggcc ttagcagcgg cgggagataa aagcaaaacc
    35881 tttaatatcg acgttctttc ttatcgggcc gaggcagaga tgatgctgga tgattttgag
    35941 gcagcaaaac acagctggga tctgatcctg gaacagggag gagatcccat ttccgggcgg
    36001 tatatgaaga gcttttgcga gagccgtctg gggaataagg accaggcagt ggctttatac
    36061 agagaggctc tggggatgga agaaaaagga aaaacatccc ctggttatga acaggctctt
    36121 ttggcagcag gagaagcctg tatggaggca ggagaccatg agggcgccaa ggtattatat
    36181 gaagaggcct taagaacgac aggaggagat gggagatttg aaagcagggt tcgctgccag
    36241 atgggcttat accagatggc tgaagaggac tacgaaggag ctgctgattc gttttatcag
    36301 ggatatgacc ggctgatcac cagttataag gcaggtacgg gagccagcct ggatcaggca
    36361 gcggcagcaa tcacagagga aaatcaggca gactggcagc ttcttaagaa gctggcgttt
    36421 taccaggcgg ctgtctgtga gtatatgcag aactatgaag aggccaaaag ccgttttgaa
    36481 acatatatcc aggtatttgg gccggatgag gcggcgcagc atgagattga ctttttaaat
    36541 accaggatac ggtagggaga tatatggcag aactgattga tttaaaggaa ctggaggagc
    36601 gggtgatcct gatcgctgta aatgtgggcg atgagaggga gacgtcagct tctctggatg
    36661 aactggaaga actggctcag actgcgggag cagtccccat tgcaaggatc gttcagaacc
    36721 gtgaagctgt tcatcctggg acctatctgg gaaaagggaa gattttagag gttcgagagc
    36781 tcctggctga cacgggggca acgggtgtca tctgtgatga tgagctttcc ccggcccagc
    36841 tgcgcaatct ggaagatgcg ctggatacaa aggtcatgga ccgcaccatg gtgatcctgg
    36901 atatttttgc ctccagggct catacaagag aaggaaagat ccaggtggaa ctggcgcagc
    36961 tgcgttaccg ggccatccgt ctggtaggca tgagaaattc tctttcacgt ctgggcggcg
    37021 gtatcggaac caggggaccg ggagagaaaa agctggagac agaccgccgt ctgatccacc
    37081 agcggattgg ccagttaaag cgggagctgg aggatgtaaa gcgccataga gaggtcacaa
    37141 ggcagcacag ggagaaaaat tacagcctga cagcagccat tgtgggatat accaatgcgg
    37201 gaaaatccac tctgttaaac cggctgacag gagcagggat cctggcggaa aacaagcttt
    37261 ttgcgactct ggatcccact acaagggcct ttgtaatggc agatggacag cagatccttt
    37321 tgacggatac ggtaggattt atccgtaagc tgccccatca tctgatcgat gcctttaaga
    37381 gtaccctgga ggaggcccgc tacagcgatg tgatcctcca tgtggtggac tgctccaatc
    37441 cccagatgga tatgcagatg catgtggtaa aggaaacctt aagagaactt gagattgtgg
    37501 ataagaccgt ggtgacggtg tttaataaga ttgaccgttt ggagggaaag gagacggagc
    37561 tgcttcccag agatttttcc tcagactatc aggtgaggat ttccgctaag acaggagacg
    37621 ggttaaagga tctggagcag attcttgggg gcattatccg cagcagccgg gtttatctgg
    37681 aaaaggtata tccctacagc ggtgcgggaa agatccagac catacgcgcc tacggacagc
    37741 ttttgtccga ggagtatacg gagcagggca ttgcagtaaa ggcctatgtg cccgcagagc
    37801 tttttgaagg gctgtaccgg gattaaacat accatcagtg gcagtattag aaaatataat
    37861 aaaacacaac atcttgttta atatataaca tatggcatag atgttgtgtt ttttgtgttc
    37921 ctctgctata atgaaaaata catttcggtt taagtgtggt agcttgtatt tggaatgttg
    37981 aaaggagttt tgaggtatga tccaggtggt aaagagggat ggggaaaccg ctgagttttg
    38041 tctgaaaaag ataacggagg caattaaaaa ggcatttagg gcaacgggga atgagtttaa
    38101 tgatgagatt ttagaactgc tttccctgcg cgtgacggct gattttcagc ccaggatgaa
    38161 ggatggaagc atttctgtgg aggagatcca ggacagcgtg gagcatgtgc tggagcagac
    38221 cggctacacg gatgtagcaa aggcttatat tctttacagg aagcagcggg agaagatccg
    38281 cagcatgaac agcgctctgc tgaactataa ggacgtggta aacagctatg taaaggtgga
    38341 ggactggcgt gtaaaggaaa attccacagt gacctattcc gtaggcggcc tgatcctgag
    38401 taattccggc gcggtgacgg caaactactg gctcagcgag atctatgatc aggagattgc
    38461 agatgcccac agaaatgcgg atatccatat ccatgatctt tccatgctca caggctactg
    38521 tgccggctgg tctttaaagc agcttctgat ggagggactg ggggggattc cgggaaagat
    38581 cacctcggct cctgcaaagc atttgtccgt tctgtgcaac cagatggtaa attttttggg
    38641 aatcatgcag aacgagtggg caggcgctca ggcgttttcc tcctttgata cctatctggc
    38701 tccctttgta aaagcagacc atctgtccta cggggaggta aaaaagtgta ttgaatcctt
    38761 tatttacgga gtaaataccc ccagccgctg gggaacgcag gcgccttttt ccaacatcac
    38821 tctggactgg accgttcccg atgatatggc agagatgaat gccattgtag gcggccggga
    38881 gatggatttt aaatataagg actgtaaggc agagatggac atggtaaacc gggcctttat
    38941 tgaaaccatg attgagggag atgccaatgg acggggcttc cagtatccta ttcccaccta
    39001 ttccattaca aaggattttg actggtctga tacggagaac aaccgcctgc tttttgagat
    39061 gacttccaaa tacggaactc catatttttc caattacatt aacagtgata tgcagccgag
    39121 cgatgtgcgc agtatgtgct gccgtctgcg tctggatctg agggagctgc ggcgcaagac
    39181 gggaggattt ttcggctcag gagaaagcac aggttctgtg ggtgtggtga cgatcaatat
    39241 tccgagaatt gcgtatctgg cagaagatga ggcggatttt taccgccgtc tggaccggat
    39301 gatggatatc agcgccagat ccttaaagac caagcgggaa gtgatcacaa agcttctgga
    39361 gcagggattg tacccctata cgaaacggta tcttggcact tttgaaaatc atttttccac
    39421 catcggcctc atcggcatga atgaagcagg gctgaataca cgctggatcc ggaaggatat
    39481 gacccagccg gagtgccaga ggtttaccag agaggttctg gatcatatga gagagcggct
    39541 gtcggactat caggaggagt atggcgacct ctacaatctg gaggctactc ctgcagagtc
    39601 taccacttac cgtctggcaa agcatgataa ggagcgctat cccgatatta tcaccgcggg
    39661 aaaggaaggg gaaacgccct attataccaa cagctctcac cttcctgtag gctatacaga
    39721 ggatattttc gatgcgctgg atatccagga cgagcttcag acccgctata cctcaggtac
    39781 ggtgttccat gcctttctgg gagaaaagct gccggactgg aaggcagcgg ccagtctggt
    39841 gcgtacgatc gcgcagaatt ataagcttcc atattatacc ctgtctccca cttattccat
    39901 ctgtaaaagt cacggctatc tggcaggaga gcactttacc tgtccgatct gtaagcagaa
    39961 ggcagaggtt tacagccgca tcacagggta ttaccgtcct gtacagaact ggaatgaggg
    40021 aaaacttcag gaatataagg accgtgtaac atatgataca ggaaaatccg tcctgaaaaa
    40081 agagccggtt tccatgaggc cggtggaacg tgtggaagcg gaatctcaga atgagagcgg
    40141 ggagggttta aaaaagaagc cccagctgta tctgtttaca accagaacct gtccaaactg
    40201 ccgcacagcc aaagaatttt taaagggcgt ggattatcag atcattgacg ccgaggagca
    40261 tccggagctg gcagagaagt ttgggatcat gcaggctcct actcttgttc tggtgcggga
    40321 cggcatggtg cagaaatttg ccaatgcgtc caatatccgc agatttgcgg aagaagtaaa
    40381 agcctgaagc cgggcagcga acattgcatg ccagaccggg catacggcaa agtgtaaaac
    40441 gtatgataca cagggtttaa aacgtccccc ggactgcggt cgaaaagact tgcggcccgg
    40501 gggtttttgg gtgataatga gtaagagtca gggctttgct ttgccaagag ccgtatttac
    40561 ggcctgtaaa agagccaggg aggtgtagcg ggaggagtca gggtatgtaa gattctccgt
    40621 tgactgggtg ttcacgattt gctctgccag gccttccatg gtgggctgca tcaggggata
    40681 catggtggcg acagcttcgc ttaggggcac cagggtcacg gcacggatct gatccttatc
    40741 aaccgttacc tctacattta cctgttcgtt tccaagggtc agggaggtgc tgtagacgcc
    40801 gggaacatag gcggtctgct gtgcctttgc ggcatctgtt tcggaagtat tttcatctcc
    40861 atggcggaac atgatgaaac agagggtgat aaggagaatg gccagtccgg cgaaaatggc
    40921 tgtgtagatg atctccttca tccgcagcac cacgattttg gttttggaac tcattgtgtc
    40981 aggacctctg agagaatttg ttacagcata tgagacggga ggataaaata tgtcattgca
    41041 gtttgttatg ggaggcgcag gttcgggaaa aacacggtat ctgtatcaaa cagccataaa
    41101 acaggccgtg gagcagccgg accggctgtt cctgtttgtt gtaccggagc agtataccat
    41161 gcagacacaa aaggaactgg tgcgccttca ccccagaaag ggcctgatga acatggacgt
    41221 tttaagcttt aagcgtctgg cctaccgggt atttgaggat ctgggagtgc aggttccggc
    41281 tgtgctggat gatatgggaa aatccatggt actcagaaag gtagccggaa tggtaaggcg
    41341 ggatctgggg ctttacggca gccatttaga gcagccaggc tttatcagtc agctgaaatc
    41401 acagatttca gagctgggcc agtacggcat ccgcccggag gatttaaaac aggtagaggc
    41461 agaaacggat catcctcttt taaaggacaa gcttaaggat ctgggagtga tctataagag
    41521 ctttcgggac tatattgagg atcactatat tacggcagag gaaattctgg atatcctttg
    41581 cagggaactt ccaagatggg aaaagctgaa aaacagcgtg atctatctgg acggctatac
    41641 aggctttact ccggtacagt accggctggc ggagctgttt atgctccatg ccagagaggt
    41701 agtatgtacg gtgaccgtgg atctgcggga aaatccctat gagaaatgcg ggatccagca
    41761 tttgttttac atgggacgtc ataccgtatg ccggttaaag gagctggcag accgccatca
    41821 tgtggaaata aaacaggaaa tccgctgtga caggcatccg ggctggcggt ttaaagacag
    41881 cgaaagcctg gattttctgg aacagaatct gtaccggtat acgggaaagg tatgggcagg
    41941 gaaaaagcct gatatccggc tgtgccaggg aaagactccg gcagaggagg ctgcttatat
    42001 ctgcaccatg gtaaaccggc tggtgcggga agacggcctt cgatacaggg agattgctgt
    42061 gattacggga gatcctgttt cctatggcag agagcttata catccctttg aggaagcagg
    42121 gattccctat tttatggatc agaagaaaag cattctggaa aatcctatgg tagagcttat
    42181 acgggcagca ctggaaacgg tccgggactg ctcctatgag agcgtgttcc gttatctgaa
    42241 aacaggactg gtatatgaca gagaaacggg gaattcagag gaaaatgaag atactcctgt
    42301 attttccagg gaaaatgtgt ctgttcttac cgatcgactg gaaaattatg taagagctct
    42361 gggaatccgg ggatggaaga aatgggatca ggagtggacc aggccttctc ggggcggaga
    42421 acggctcaat ttaaaggaat taaatcagta ccgccagtgg gttttagccc ctcttaagga
    42481 gctgcgggaa gcattttcac aggaaggagc ctctatttct acagtgacat ccgccgtaag
    42541 aagcttttta gagaagatgg aacttaagga aaagctggag gaatatgcag agttttttga
    42601 aatgaggcat tgccggggag atgagaatct ggcaaaggag tatggtcagg tctatgaacg
    42661 tgtggaggag ctgcttttgc gtctggaggg gctgttgggc gaggaaaagg cagaccggaa
    42721 aaattatatc cagatactgg aagctggatt tgaggaaatc cgggtgggcg tgatccctgc
    42781 tacggcagat caggtgatga tcggagatct gacccgaagc cgtctggaat cggtgaaggt
    42841 cttatttttt gccggattaa acgaaggcct tgtacctcag agaaaatcag gaggaagtct
    42901 tttgacagat acagaccggg aaatcttccg ttcctttgat atggagctgg cgcctacggc
    42961 cagagaggac agctgtattc agaaatttta tctgtattta atgctgtgca agccctcacg
    43021 caaattgatc cttacctggg ctgcctcttc gggagacgga aagagtatgc gcccctccag
    43081 cctgatcgga gaggtaaaaa agctgtttgg ggagcttcgg gaggaaatct gctttgaaga
    43141 gggatatccc atccagacga tctgggacgg aagacaacgg cttataggga tgctgcgtga
    43201 ggccagggaa agtcaggaaa agaagccggc atttttagag ctgtaccgct atttttacag
    43261 tgaggaggcg tacagggaac aggtaaaaca gctggtgaat gcggcctttt ttacctatga
    43321 ggaccgcggg atcggacggg ctgcagccag agcgctttac ggaaggactc tggagggaag
    43381 cgttacccgg ctggagcagt tcgcttcctg tgcctatgcc cattttctga aattcggcct
    43441 ggagctcatg gagcggcagg aataccggct ggaggcagtg gatatgggaa atctgttcca
    43501 ccagtccatt gacctgtgct ttcaggcgat ccagagcagg aagctggact ggaaagggat
    43561 tacggaggct gagagaaagg ctctggtaag ggaatgtgtg gagcaggtga caggccagta
    43621 tgggaatacc attatggaaa gctctgcccg caatgcctat ctggcccgcc gggtggaacg
    43681 gatcacagac cgtaccatct gggctctggc agagcaggtg aaaaagggag attttgtacc
    43741 ggcaggtttc gaggtgtcct tttccgccat tgatgatctg aaggccatgc ggatccggct
    43801 gtccgaggac gaaaagctcc agctgaaggg gcggattgac cggctggatc tgtgcgagga
    43861 tgaggagcat atctatgtga aaatcattga ctacaaatca gggagcacct cctttgacct
    43921 ggcggccctt tattacggcc tccagcttca gctggtggtg tacatggatg ctgcattaga
    43981 gatgcaggag cggaagaatc cgggaaagga ggccgtacct gcggggattt tctattacaa
    44041 cattaaggat ccggtagtgg acaaggatgg ggaaatgagc ccggagttta ttgagaagca
    44101 gattttaaag cagcttcgga tgaatgggct ggtaaacagc gacctggatg tgatccatca
    44161 tctggatcgt gagatggtga aggattcgga tgtgatcccg gttgtattcg gaaaggacgg
    44221 aggcgtgcag gaatcacgtt ccagcgtggc aaaggcggtg cagtttgaaa cccttcgcgc
    44281 ctttgtggga cggaaactga aagcttcggg aaaacagatc cttaacggcg atacggcgct
    44341 tcgtccttat aagcagggaa gccgtactgc ctgtgattac tgtccgtatc atgcagtctg
    44401 cggctttgat accaagacag caggctatgg cttcaggcag ctttgttcca tgagcccgaa
    44461 cgcagtatgg gaggccatcg agagagagga ggaagaaaat ggcagtgaac tggactgatg
    44521 aacaaaaaca ggtcattaac agccggaaca gcagtctttt agtctctgct gcagcaggca
    44581 gcggaaagac ggccgtactg gtagaaagga tcatttccat gatcagcgag ggggatcatc
    44641 cgttaaatat tgaccagctt ctggtaatga cctttaccaa tgcggcggcg gctgagatgc
    44701 gtgagaggat tggcgcggca gtagaaaaaa agctgcggga gcatccccta gatgagcacc
    44761 tgtggcttca ggctgcctta attccccagg cccagatcat gaccattgac agcttctgcc
    44821 tgaatctcat acgaaaccat tacaacagcc ttgacattga cccgtctttt cggatcgggg
    44881 atgagggaga gctgacccta ttaaggggcg atgtgatgga ggagctctta gaggcgtgtt
    44941 atcaggagga aaatgaagat tttatccggt ttgcagagca gtttggggcc ggaaagtcgg
    45001 ataaggccat ggaggatgtg atcgtacagg cctggcagtt ttcccagagc catccatggc
    45061 ccggcgaatg gctggacgaa tgcagacggc agctgaaaca ggaggcagag ggaaatctgg
    45121 atgaaagccc gtggatgaaa ttccttttaa aggacacggc gctgcagatg gaggagctta
    45181 cacatcagct tcaggcggcc ttggaggtgt gcagggagga agggggacct gccgtttatg
    45241 agcccatgct ggaaaatgac cttgcagggc ttttaggaat taagaaggcg gcggaaagag
    45301 gaacctttga gaagctttat gaagcccttc aggctatgga gtttgtccgt ctggcagcgg
    45361 ccagaaacaa ggccattgac gaggataaaa aggagtatgt aaaggaatgc cggacccagg
    45421 tgaaaaagct ggttgaaaaa tgccgggaaa gctttggctt tcagagtcca aaagaagcgg
    45481 cagaggcgat ggcaggggct gctcctgtgg ttgaaacgct ccttgatatg gtacagaaat
    45541 ttgacgattt ttaccggaag gcaaagcggg agcggaatgt acttgatttt aacgatctgg
    45601 agcatctggc cttagaggtg cttcttgagg gagattctcc cagacagcct tcggcagtgg
    45661 caaaagagct tcagaggcag tatgaggaga tcctggtgga tgaatatcag gacagcaacc
    45721 tggttcagga aacattgatc gggagcattt caagagaaga aacgggccat cccaacgtgt
    45781 ttatggtagg agatgtaaag cagagcattt accgcttccg tctggcgaga ccggagcttt
    45841 ttatggagaa gtatgcatcc tataccagag aaaaaagccc tcaccagatg attgaactca
    45901 ggcagaattt ccgaagccgg gacacggtgt tagcctttat caatgacatt ttttaccaga
    45961 tcatgacgcc ggggctgggc ggtatccggt acaccgagga aaccgccctg tatccgggag
    46021 ctgtttttgc atcggcagag ggaatggccg gagttccatc caggcttttg atggcggata
    46081 cgggatcaga tgcctataaa cagctggatg aggatgccat gggatatacg gcaagagaaa
    46141 tggaagcaaa gatgattgca ggagaaatac gacggctgac tgatgaaacg gacggccttc
    46201 ttgtgtggga taaggagcag gagaaataca gaagggtctg tctgggagat atggtgatcc
    46261 ttcttcggag catgaccggc tggtccgaga ctttagtcag cgtgctgatg aatgagggaa
    46321 tccccgccag agcccagaca cgcaccgggt atttttccac cacagaggtt gaaacggttt
    46381 taagcctgct gtccgtgatt gacaatccca tgcaggacat tccccttgca gcagttttgc
    46441 gctcccctgt agtgggaatg agtgatgatg agatggcctg gatgatggcg tcctataaga
    46501 aaaacacaaa aaaaggccag gacaggggcg tatatggagc atggaaactg tgggaggaag
    46561 aacgggagca tccgggtctg agagaagctt tggagcatcc gggtggagca tttgagaatg
    46621 aaaccggaga ctccgaggat gcagtccgga taggcgccgc cgttgtccca gtggagcagg
    46681 ctgcacttat atggaaaaaa ctggaggatt tcggagcgct tttaaaggag ctgcgcacag
    46741 aggccagata tcgccctatc cacgaactgc tgtattatgt atacaggcgc agcggatatt
    46801 atgattatgt atctgccatg cctgcaggag agatccgccg gggcaatctg gatatgctgg
    46861 tggaaaaggc agcggcttat gaggcgacca gctacaaggg tctgttccat tttgtaaggt
    46921 atattgaacg gctgaaaaaa tacgatacgg atttcggaga agccgccgtg gctggcgagg
    46981 aaaaaaacat ggtccgcatt atgagtatcc acaaaagcaa gggactggaa tttccggtgg
    47041 tatttctggc aggaatggga aaaaagttta atcagcggga tgcttacggc actcttcttt
    47101 tagatgcaga catgggggcg gcaaccgatt ggctggatct taagctacgc acaaagactc
    47161 ctacgctcaa aaaacaggtt ttaaaacggc ggctggaact ggaaaccatg ggagaggagc
    47221 tcaggattct ctatgtagcc atgacccgtg caaaagaggc gctgatcatg acaggaacgg
    47281 accgcagcct ttcaagtaag ctggagcgct ggagaatcgg aaggacggag ggaaaacagc
    47341 ttccattcac tcttttatcc ggagcaggtt cctatctgga ctggattctt atggcagccg
    47401 gggcagtgcc gcaggaacat tttgaaatga tcaaggtgca gcccgaaaca ctggtgggag
    47461 aagaggtaac aagacagatg gaacggaaaa tggcaaagga ggatctgttg gaactggatg
    47521 caggttatac ctatgatgaa gagatgaaaa aggagctgga acaggcattt tcctatgctt
    47581 acccttatga aagcgataca gggctttaca cgctggtttc tgtgtctgag cttaaaaagc
    47641 agagccagat cggccgggaa gaggaggcaa tgggaaccgg ccgggaagag gacggctgga
    47701 tcagggatct ggacgaggaa aaagcagaag actcggtcaa agagacagaa gcagggggga
    47761 cggttcaaga agcagaagcc tctgaagcag gagcgaaaga agggaagaag gtttatggcc
    47821 ggtcaggcgg ttctaagaga ggtactgcat accaccgggc gctggaatgt ctttccttcc
    47881 atgaactgca cagcctttca gatacaaaaa aggccctgtt ttcgctctat gagcagggat
    47941 atctggatca ggagtcttat gagtggatca acccctggga tatttggaaa tttgtggaaa
    48001 gcccgctggg gaaatccatg gcaaaggcgc aggcagaggg gcggcttttt aaggaacagc
    48061 agtttatgat tggtgttccc gcttccgaaa tgggccatga ggactctgaa gagctggtgc
    48121 tggttcaggg agtcattgac gcttttatcg aagagccgga gggacttctg ttaatcgatt
    48181 acaagacaga caggatatcc ggaaaggata aagaaatggg gaaagagcgt ttaaaggagc
    48241 gctaccggac tcagattgac tgctacgaac gggcattgtg ccagatcaca gggaaaacgg
    48301 tagcgcgaag gtgtatctac tcctttgcat taagagaaga gatagagatt taacccgaaa
    48361 cggtcccggc tgcatcagac tgcaaccggg attttgtgct caaagggatg gaactggtag
    48421 ttttccagtc ggaaactgtc cgtggtaaac tgatagaaat ccttcacttc aggatccacc
    48481 caaagcgtgg ggccttcaaa gggctgtttt ttaatcagtt cttctaccat ggggatatgg
    48541 cggtcataga tatgggcgtc tgcgatcaca tgaaccagtt ccccggcctt caagccgctt
    48601 acctgtgcaa acatatgaag aagcactgcg tactggcaga cattccagct gttggcagtg
    48661 agcatatcct gagaacgctg gtttaagatg ccgtttaagg tattccctga tacattaaag
    48721 gtcatgctgt aggcacaggg atagaggttc atctcagaga gatcatggtg attgtagatg
    48781 ttggacatga tccggcggct taatggattg tgctttaagt catagaggat ccggtctacc
    48841 tgatcgaact ccccttcttt gtaatggtgc tttacgccca gctggtagcc gtaggccttt
    48901 ccgatgcttc ccgtttcatc cgcccaggaa tcccatatgt ggctttttaa gtcatggata
    48961 ttgttggact ttttctgcca gatccataaa agctcgtcta cagccgattt aaacgcggtt
    49021 ctgcgcaggg tgataacggg aaattccttt gtaagatcgt aacggtttac gatgccgaag
    49081 cgcttgatgg tgtgagccgg agtcccatcc tcccataccg ggcgtacctc atggtctgtg
    49141 tcccagacgc cgttttctaa aatatcttta cagttttgta taaaaagctg gtctgcatag
    49201 ctcatagatt gtatcctcct gatggcagtg atagtatgtt gcagttctga gtatagcaga
    49261 tatggggccg ggtgacaata tggagatatt tgagtttccg cattctgcac cgcaccctgg
    49321 agtctgctgt gccgggttcc ggtttccagt cataagaaaa tctaagaggc tgaatttttt
    49381 ctaaaagcaa aaaccatatc aaaaactcgc tgcgctcata caggtttgat atggctttta
    49441 acgaaaaaat tgatcctcta aggttttcta atgacctcca aagtcacccg gcacagcagg
    49501 ctccagggcg cactgcaaaa tgcggaaact caagggagat atctcaaaac atttacaaaa
    49561 cacagggaaa acggtatact ggggacagca tattatgagc agaaggaaag gcaggaaaga
    49621 gtgatgacac agaaacggat tcgggattat ggtatcatgc cggggcggat aaagacggga
    49681 aagaggaata agataacgga tgttcccgga gtgcgggtag gacactgtac ggtaaaagaa
    49741 ggagagattc atacaggcgt tacagtgatc cttccggggc cggataatgc ctttttaaat
    49801 ccttatacag cggccgccta tgtgcacaac ggttttggaa aaagctgcgg actgatacag
    49861 attcaggaac ttggtacgct ggaaactccc attgccctta caaatacgct gaatgtgggg
    49921 aaagtatggg acgctgtggc agaatatgtg cttaggcagt gtgaaaatga cggtgcagag
    49981 gctttaagca tcaatcccgt ggtaggagaa tgcaatgaca gccgcattaa ccggatttcc
    50041 ctcagggcca ttggggcagg gcaggtaaag aaagcctttg acgctgccgg ggaagatacg
    50101 gaggaagggg atgtgggagc cggagccgga acaatctgtt atggcttaaa aggcgggatc
    50161 ggcacatctt ccagagtcat aaaggtggga gagaaggagt ataccatcgg cgtgctggtt
    50221 cagtccaatt tcggggcaac ggaagatctg atggttgacg gcagacccct tggacgggaa
    50281 attctggaaa agtatggaaa tgaagcagag gatccggtga aaaagacggc tttttctgaa
    50341 caggataagg gatccattat gagtattctg gcaacggatc ttccggtgac agacaggcag
    50401 cttacccgta tcatccgccg tctgggagtg gggattgccc gtacaggcgc ctacacaggc
    50461 cacggaagcg gggaagtgat gatcggcttt actacagcga accgtgttcc aacgaaaggg
    50521 ctccgaagag aagaggaaga gcttcgtacc ctgactgcca tccctgagga gaccatcaac
    50581 cgtgcatttc tggcagcagc agaggcagaa gaggaagcga ttctcaattc catggcagcg
    50641 gcaggggaaa cgagggggct gaagggggaa tactatcgca gcctgagtga cattctgtcc
    50701 caggagggag aaattcattg aattcctata agacgttgat cccgcgtatg actttacagg
    50761 aaaaggcggc gcttttaggc ggcaagggag agtgggaaac cagggatatt cccagactgg
    50821 ggattccttc tatggtgtgt tccgacggcc ccagcggtct gcgccgccag gccggaacgg
    50881 gagaccattt aggcctgaat ccatcccttc cggccacctg ttttccgtgc ccttccctta
    50941 tggcctgcag ctgggatcag gagctggagg aagcgatcgg agctgccctg ggggaagaag
    51001 ccgcagatca gggtgtggat attcttctgg ggccgggatt aaatatcaag agaaatccgc
    51061 tctgcggccg gaattttgaa tatttttccg aggatcccta tttttccgga aaaatggcgg
    51121 ctgcctttat ccgcggagta gagagccagg gaatcgctgc ctgccccaag cactttgcag
    51181 taaacagcca ggagctgcgg cggatgacga tgaacgcggt ggtggatgag agaactctgc
    51241 gggaaatcta tctcaccggc tttgagaccg caataaaaga gggaaaatgc agggcgctca
    51301 tgacttccta taatcaggta aacggcatct atgccaatga acatccccat cttttaaagg
    51361 aaatcctgcg aaaagagtgg ggcttttgcg gagccgtcat tacagactgg ggcggctcga
    51421 acgatcactg tgccggagtg aaaaacggtt cttctctgga aatgccggct cccggcttaa
    51481 gctctgccgc agcgctttta aagggagtag aggaaggcag gattacggaa caggatctgg
    51541 atgaacgggt ggaagagatc ctgaacctgc tccctggaca gaagaaaatg ttcaaacaaa
    51601 ataaaatgcc agagaattct ccggagaggg aaccgcttta tgaagcccat caccatctgg
    51661 cgcgcagagc ctgtgcagag agtgtggtgc ttttaaaaaa tgaaacggat accctgcctc
    51721 ttaaatccgg tacccggatt gccgtaatcg gcgattttgc ctatgaatcc cggtatcagg
    51781 gggccggttc ttccatggta aatgcctgga aggtggattc tgtaaaggaa tgtctggaag
    51841 gggagacggg gcttgaatgg aagaaaatgg gaatcactgt ttccggtttt tcaagaggat
    51901 atctccggga tggaaggcag gatgcagcgc tggaaaaaga ggctgttgac ctggcaaaaa
    51961 cggcagatca gatcctgttt ttctttgggc tcaatgaagc ctgggagacg gaaggaatgg
    52021 atcgcagcca tcttaggata ccggaaaatc agattcacct tttagagcgg ctttcccgcc
    52081 tggagcttcc ggttgtaggg attctgtgta caggaagtgt ggtggaaatg ccctgggaag
    52141 gccatctaaa ggcgcttctt tacggaggac tctggggcca ggcgggagcc ggcgccgttt
    52201 tagaccttct ttcaggaaaa ttaaacccat gcggaaagct ggcagaaagc tggccggtga
    52261 aatacgaaga caccccgtcc tttcagtact atccttccaa agagagaaat tctgagtacc
    52321 gggaggggat ttatgtggga taccgctatt atgacacagc aggcgttcca atccgttttc
    52381 cttttggctt tggactgtcc tatacaaaat ttgactacag cggtttaaag atcacacctg
    52441 agggggtgtc ctttgtgctt gaaaatacag gcagcaggga cggcgcagag atcattcagc
    52501 tctatatagg ccttcccgga gcacgtattt ttcggcccaa acgggaatta aagggctttt
    52561 gcaaggtgtt tttaaaggtt ggggagaaaa aagaggtgtt tcttcccttt gattctatga
    52621 cctttcggta ttggaacgga agaaccggac agtgggagag ggaaggaggt ttttaccgca
    52681 tcagcattgg ttccgggctt acggatatcc gtctggaagg ggaagttttt caggagggaa
    52741 ccacagacag ccttccatgg gaggtgaacg agcttccctc ttatgcttcc ggccggatcc
    52801 ggtctgtgcc ggatgaggag tacgagcgcc tgttaggctg tcccattccg gacggaagct
    52861 ggaaggggga actggggatt aacgatgctc tctgccagat gtattatgcc aaaagtccgc
    52921 tggcaaggct gatatgccgg attttaaagc accgccttga gaacgcgcag aagaaaggca
    52981 ttcctgattt aaataccctt tttcagtata atatgccgtt ccgggccatg gcgaagatga
    53041 caggaggctg gatttccatg gaaatggccg aatcgttaac ctatgcggca aacggccgcc
    53101 atttttatgg tatcactggc tttataaggg gatttttaaa aaacaggcgt ctggagaggc
    53161 ggtatgagca ggcgctgaaa ggtaagacgg cgccgaatgt gggaaaccca tggagcaaag
    53221 cccatccgca gctgtgggaa tttatcctgt ttaatcttct ttccaactgt gctaccatta
    53281 ctaattttgt ggtaatgtgg ctgtgtacag gctgggtttt taaggggctg gcagacattc
    53341 ccttccggtt tttcatattt gattatgggg aagatgcatt ggggctttgc ggttttctaa
    53401 gctttcttac agccacagct ctggcccaga tggtaaattt ctttgttcag aaaaactggg
    53461 ttttccggtc cgatgccgca tttagcaggg cagcgccccg atatatcctt ctggcagtgt
    53521 ttcttgtgat cctttcggct gccctgccgg cctggagcca gaagcttttt acaggtttgg
    53581 gagtccctga agcatttgct cctacctgtg ccaacgggct taatatcctg gctcaggtgg
    53641 cgatcagtta tcctgccatg aaattctgga ttatgcctgg tcaggcccgt ccaaagaaaa
    53701 ataagaaaaa aaggagaaaa aaatgatgca gtttgagaaa tatacagaat atgccgtaga
    53761 acaggcaaag aagcttttag ccatcgacag tccttccgga tatggctatt atgtgacgga
    53821 gtatttatta aaggagtttg ccgctttggg gattccggcc cgccggacca taaaaggcgg
    53881 agtgattgct gactttggag gaaaaaagga tgagaacggc atccttctgg aagcccactg
    53941 cgacaccctt ggaggtatgg tttcccagat caaggaaaac ggccgcctta aactgaccaa
    54001 tatcggcggc atgaagcctg ccaatgcaga ggctgaaaac gtgcgcatca ttacaaaggg
    54061 agggaacgtc tatgagggcg tctgccagct tgtcaatgcg tctgtccatg tgaacggaaa
    54121 ctacgaaaca acggagcgca gctgggatac agtagaggtt ttagtggatg aatgtgtaaa
    54181 aacaaaagag gataccgtga agctggggat catggcaggt gattatgtat gctttgagcc
    54241 gaggaccagg gttacagctt caggctatat caagagccgt tttctggatg ataagctgtc
    54301 tgcagcgatc ctgctggcat acggacggta tttaaaggag gaaaaggtgg ttcctgagcg
    54361 ccatgtatat gcgcatttta ccatttatga ggaagtggga cacggaggct ctgcttctgt
    54421 gcctgaaggc gttacagagg cctggtcggt ggatatgggc tgtgtgggag acggacttac
    54481 ctgtacggag catgaggttt cgatctgtgc aaaggacagc gggggcccct acaattatga
    54541 ggttgtctgc cggctggtgg agctggcgaa ggagcatgag atcggctatg ctgtggatgt
    54601 atatcctcat tatggctccg atgtggaaac cacgctccgc tctggtcatg acatccgcca
    54661 tgcgttgatc ggtcccggcg tttacgcgtc ccatggttat gagcgcagcc ataaggacgg
    54721 cgtctgcaat acattccatc tgatccaggc atatctggga tagggaatat gaaaaaggga
    54781 ggctccatat aatatccaga ggataaaaaa ggagcttctt tttttatgga caggcgcatc
    54841 ctgtttattt tgatcatgtc gctggctgtt ccctatggct gttctatggc tcttttaggc
    54901 acttctttgg caaagtccgg cgggcttaag gaacaggagg ctctttcggc tgcttgggct
    54961 tctgattccc gggaaaaacg gtcttttcgc agaatccgca tggacatgga agatccaccg
    55021 gaaaaaacgg tagatctgga aacgtatctt cccattgtgg tggcaggaca gatttcggca
    55081 gagtctcatc cggaggcgat aaaagcccag accattttgg caaggaccta tatctgccgt
    55141 cagatggaac agatgggtct tgaggaggaa ataccggaat cttctcttgg actggaaaaa
    55201 aatagaaata atcagatgga gccgctttgg gggacagaaa tttttcctga atattacagc
    55261 cggtttgaga aggcagccag agagacagaa ggggttacgc ttacctggga gggccatttt
    55321 attgaccctt tatttacgca cacgacgacc ggttttaccc gtccgggaga tgaggaacat
    55381 ccctatctgg cttccgtatc ctgtgtccat gatcctgagg aggagggatt tgtgcaggaa
    55441 atgatcatgc agccggaaga ggccgcaaag cgtgtgtccg caattccggc tggggatgga
    55501 tcgtttcgta ccataccgcc ggaagcattt cccgaacagg tgcagattat ttcaagagac
    55561 gaagccgggt atgcactgca gcttcagatc gggggctttc cctttaacgg ggaagaagtc
    55621 cgcctggctc tgggccttcc gtctgcctgc tttgaactga accgtcatgg cagtgatgtt
    55681 cgttttacgg tcaggggaag ggggcatggc tggggcttaa gccagacgga ggctcaggct
    55741 ctggcagagg atggatggag cgcagaggca attttggaat atttttatac aggcatttca
    55801 ttttcttctg aataagtcac ctaaatccgg taagaatact accgaggtga taaaaagatg
    55861 aaagagaaag tcaatcagat gctcaaggat aagctgtttc tcgtgatgct ggtattagga
    55921 ctgctgacta tggtagccgc agcaggaatt atgaccgtac aaaaagggaa aaacagtgcc
    55981 aatgacccca tgctccatat ggcagaggag gaaaatacgc ctacagagga agcgctgcag
    56041 atggcgggcg ccagtgacgc cagagaaaaa acacaggaag aagacaggga agaaacggtt
    56101 ggagccgacg caggggaaaa tcccaatgaa gggaatgatc tgcttgcaga ggcgaaagaa
    56161 caggatgggg cagcagaggc aggagccgga aaggaagcgg ctcatgcatt ggccttaaac
    56221 tttacggaat ccagctccat ggtatggccg gtccggggaa acgtgctgtt ggattacagc
    56281 atggatcaga caatctattt tcccactttg gatcagtaca aatgtaatcc cggcattgta
    56341 atacagtcgg atgtgagcgc tccggtagct gctcctgcca atgccagggt aacggaagtg
    56401 ggagtcagcg atgaaatcgg aaattatgtg gtcctggatc tgggaaatga gtacaccgct
    56461 gtctgcggac agttaaagga gatttctgcc gcagagggag aatatctgga agcaggccag
    56521 actttaggat atgtagccga gcccaccaag tattattccg tagagggagt aaatgtattc
    56581 tttgaattaa agcacaaggg ggaaacggta gatcccctaa accacatgga gtgaagccca
    56641 gcctgtccgg agacataaaa ccgggcgggc tcttttcgtt tgaagctgag aaaatgggaa
    56701 agtttggaat gaattttata aaatttaaga atttgttcat agcactttca tgtcattatt
    56761 gtataataaa tataacaacg aaaagaggag tgaaacaatg aacatattat ttttccttac
    56821 tcccaagagt gaggtggctt atatcaatga ggatgacaca ctgcgccagg ccctggaaaa
    56881 gatggagcac cacagatatt ctgccatccc tattatcagt cgtacgggcc gctacatagg
    56941 aacgcttaca gaaggggatc tgctctgggg aattaaaaat cagtatcatc tggacttgaa
    57001 gggggctgaa aagatcccga tcacagcgat ccaccggcgt ctggacaacc gtcctgtaaa
    57061 ggcaggcgct gatatggagg atctgatggg aaaggcactg aatcagaatt ttgttccggt
    57121 gttagatgat cagaagtgtt ttatcggcat tattacccgg aaggacatta tccagtattt
    57181 ttataagaag acccagcagg cccaggaaga tgtaaaggca tgctgcggat cgggctatgc
    57241 agagaacaag aagcgggatg tggcttctgc tgttgcattt caatcagaga aagaacgagc
    57301 gaacgaaaaa accatgtgaa aatgcataaa aacaaaaaag acatttttta aagtttgtgc
    57361 acacttttag gggcggatat gctattataa tagacgtaac ccccccaata cattatatag
    57421 ttttgctaca ccccataaga aacgaaatac cttctcctga aaagaccggt tattccgcta
    57481 ttaaatatct gaaaattttg tattgacgga atgaagcctt tggtgtatat tttattacaa
    57541 tgcagcaaaa tgtaagagag tggctgacag atgtatgtct aataaataca ctccaaggcg
    57601 tatctgccgg tttcagatga gagtttgttc ggctcagctg aatctgcgat gccctgagag
    57661 gaatatgagg aggaaattat gaagccatat gcagagatga gcaaagagga actgacggct
    57721 cttcgcagag agctgaaagc aaagtaccat gagtttcaga ccagggattt aaagctggat
    57781 atgtccagag gaaagccaag cgttgaccag ctggatcttt ccatgggtat gatggatgtg
    57841 ctcagcagcg acgatgatct gacctgcgag gatggaacgg actgccgcaa ctatggtgtt
    57901 ctgacaggta ttgatgaggc caaggaactc ctggcagaca tgatggaggt aaatccttcg
    57961 acgattatca tttatggaaa ctccagctta aatgtaatgt acgataccgt ttcccgggcc
    58021 atgacccacg gtattatggg aaatactccc tggtgcaggc aggataaggt gaaattcctc
    58081 tgccccgttc ccggatatga ccgtcatttt gctattacag aatatttcgg tattgagatg
    58141 atcaatgttc ctatgacttc tgaggggccg gatatggata tggtagagcg tcttgtagct
    58201 tctgatccgg cgatcaaggg catctggtgt gtgccaaagt attccaatcc tcagggatac
    58261 tcctattcag atgagacagt gcgccgtttt gccagactgg agcccgcagc gcctgatttc
    58321 cgtattttct gggataatgc ttatggcgtt catcatttat atgaccacga tcaggatcac
    58381 ctgattgaga tcctggcaga gtgcaagagg gcaggaaatc ccgatctggt atataaattt
    58441 gcttccactt ccaagatcag tttcccaggc tctggtattg cggcaatcgc tacctctcat
    58501 aataatcttg aggatatcaa ggcccagtta aagaatcaga ccattggaca tgataaggta
    58561 aaccagctgc gtcatgtgcg tttctttaag gatatccatg gtatggtgga gcatatgaga
    58621 aagcatgcgg atattatccg tccaaagttt gaggcagtag aagaaattct ggaaagagag
    58681 ttaggcggtc ttgggatcgg agaatggact aagcccaagg gaggatattt catttccttt
    58741 gattctctgg atggctgtgc taaggatatt gtggcacggt gcaagaaggc aggcgtggtg
    58801 atgacaggcg caggtgctac ttatccatat ggaaaggatc cccatgacag caatatccgt
    58861 attgcaccaa cttatccgcc tctgggagat ctaatcgtgg ctaccgagct cttttccctg
    58921 tgtgttaagc ttaccagcgc agagaagctt ttaagggaaa tggagcagaa tccacagcct
    58981 gtaaagaaaa aagaggcaga ccagcaactg ccagagtaaa aataaaacag cttcagatac
    59041 taacagggag ttcaccgttt cggcggggaa ctccctgtgt gcgtgaatga ctggttcacg
    59101 gatggttttc cgggcagaga aaagctgcag gaatgactca gaaaggagcc gattgatgga
    59161 agaagcggga gtttatactg gaaaatggga ttttgttacc ttttttgtgg ttccgggagc
    59221 tacattgtgg ctggcttgcc gggaggattg gctggacagt aatatctcgg tcatcgggaa
    59281 tagtcccggt atggaactct ggctgtatgc ctgggggatg tttgtcagca gctacagttt
    59341 tttctatata agatatctgt tttctctggg cggatatgag ggcattaaag gaaggggatt
    59401 tttgtatacg gcctgtatcc ttctggctat atccgtatgg atcccctatg atccggagca
    59461 ttttccaaag gcctctttac tccatgtgat tttttccttt tcttttcctg ttcttttatc
    59521 cctgtccctg ctttcctttt taaaagaaaa gatccgctca gatcccaacc cgtttcttcc
    59581 gatactctgg ctgtttgcag agcttgcggc agcggctctt gtactgtggt atgcctccgg
    59641 ctttatcaca ggccttttgg aagtgtatgt aaccactgcc ttttgtggat atctgggttt
    59701 ggttgagcgg atcctggtgc ataaccaaag atcattccct gaaaacaggg gagattagaa
    59761 ggttacttcc tcatcataga agcagcactt taacagtttt gccatctgct ccggatcgat
    59821 ggatcttggg ttggagccgg tgcaggcatc gcctactgcc agttcagcta cttttggaag
    59881 tttttccata aattcagctt cgtcgatgat tccgccttcg taatccttaa tgcaggctgg
    59941 aatgtctaac tgctcattca tttcgcgcag gcgggcgatc agagcgtctg taagctccag
    60001 atcggtgctg ccggccagtc cgatgaattt tgcaattttt gcatatcttt cggcagcctc
    60061 aggatttttg gaattgaatt tgattacctt tggcagatac attgcgtttg cacagccatg
    60121 aacgatgtgg ccgccggagt atgcggcgcc tgtcttatga gccatggagt gaacgattcc
    60181 caaaagagca ttggaaaatg ccattccggc cagacactgt gcgtcatgca tcttggcacg
    60241 ggcatccata tctccgttgt aagatggaac cagatactcg ttgatcatct caatggcatg
    60301 aagtgccagc ggatcggtat attcgcagtg aagggtagaa acgtacgctt cgattgcatg
    60361 agtcatagcg tccatacctg tatgagcagt caatttcttt ggcatggtag ctgccagagc
    60421 gggatctacg attgctacat ccggtgtgat gttaaagtca gccagaggat atttgatccc
    60481 ctttgcataa tccgtgataa cggagaatgc agttacctcg gtagcggtgc cggaggtaga
    60541 ggaaatagcg cagaactttg cctttgtgcg cagggttggg aagctgaatg gtgtgatcag
    60601 atcctcaaag cttgtttcag ggtattcata gaaggcccac atagcctttg cagcatcgat
    60661 tggagaaccg ccgcctacag ctacgatcca gtcaggctgg aattcttcca tagcctttgc
    60721 gcccttcata acggtttcta cagaaggatc cggctcaact ccctcaaaca gctgaacctc
    60781 catgcctgct tcctgtaagt aagcaactac cttatccaga aaaccgaaac gcttcatgga
    60841 gccgccgcct actacaacga tcgctttctt tcctgttaat gtctttaatg cctccagagc
    60901 atcctttccg tgatacagat ctcttggtaa tgtaaacctc atgagtaaaa cctcctgtga
    60961 agaatgatat ttatttaaca aacatagaat atcacaggaa aagaggttcg ggtagatatg
    61021 aaaatggaat acaggtatgc cggaatggat atcctttatc agcttgtcct tatcagaacg
    61081 gagactgtga gggagaaggg agcgcttaaa tcagtccgaa agccggctta cgatgcggtt
    61141 cagactatgg atcagttcgg tttccgccga agagcggcgg ctgccctttc gaaagatcag
    61201 aagatccctg tgcagatttc cgggataagc gcagggacgc tggatcaggc cttcggcaga
    61261 cagttcgctg tagggaatgg gagaggtcca catataggct ccgggcagac ggcgcagcag
    61321 ggcaaactgg cttccgcggt cataaacgga cacagaacgg ctggaatcgc ctcctgtggc
    61381 tgaagtttcc tggatttcaa aggaaaaaga gggattggag gaatcgccct ggatgatttc
    61441 aagagagctt cccagatcca gataggtcag gccggatctg cctgccagag gatgatccgg
    61501 agccattaaa accagggatg aaaattccca taaaaggcag gagtccagat tttcatcctg
    61561 gagcagcttt ttatagtaag gttcatataa agcaggacag cgtgcgatcc ccagatcaaa
    61621 ttccttggaa gataccagct caatgacctg tctggagttc gtttccatat agtggatatt
    61681 taccggggat ttggtgttgg cttcacagag aaaatccgta aatgcacagg tgatatatgt
    61741 ggctctgggg ccgcagaact taagctccag agcctgtttg cggtttcggg cgcagagggc
    61801 ttcgatctgt tcctcctgag ccatgatggt tttggcgtac cggagaaatt cttcgccctt
    61861 tttagtggga atcatgcctc tgctggtccg gtcaaaaata tcttctccca gctcccgctc
    61921 cagttcccgt atgattttgc ttaagtgggg ctgattcata taaaagttct gggcggcttt
    61981 ggtgatagag ccggtttttt ctacctctac tgcgtatttt aaataggaaa gattcatgag
    62041 tcacctgcct tcttttaaga ggaagccgtt ggctgaaaag ggatgatagg gtttgttttt
    62101 ggagtatagc tgtccggcat tccgtctacg aacggagcat cggagggaag cgtttcggct
    62161 ggagcaggat cggaagaagc cggatgctca gggcctgttt tggtctcata gggggcagcc
    62221 gaaggcgttt gctccgcctt tgaagtttct gaagaagcag tatttctgtg gataaccgca
    62281 gcatggcctt tataggtctt ggaatgatcc agtatgcgtt ccctttcttc cccgtctttt
    62341 gatattacct tataggtgtc ccagcggctg ccgggaaccg ctgcctttac caccttttct
    62401 gttccgggtt caagtgaagg atcctccgca tattccggtt ccggaggagg cagttcctcc
    62461 accttttcat aataaagatc ccaggaaacg ccttcttcca aaacagggat cccatagatg
    62521 gatacagtgg ctttgcggtc cgcatagctg gcccggatgc caatgggggc actggaggtg
    62581 tttacaaaac ggaaatccgg ctgttcccag ctgatggtcg catcctgtcc aggagtcaca
    62641 tagtttggct caaaggtgtg agaacggcgg aaggtaattt ccattcccgc attaaacgct
    62701 gcccggtaca gggtactgga aatctggcag acaccgcctc cgatttcctg taccacagcg
    62761 ccgctgctgt atgcagctgc ttcctgatag cctttttcag ccgtgcgctg gcccactgcc
    62821 tgattaaagg aaaattcctc gccgggctgt acaatggtgc cgtttaaggc ctctgctgcc
    62881 agccgtacgt ttgtgttgcg tttttgattg ctggtggttt ccgtagtaaa ggcagcgatg
    62941 gttttgtact gctcttttgc catggccttg gagagaggcg gggcagcgtc tgcggaatga
    63001 acggggatta ctgcatccag ccggttttca cggatagcgg aaagaatatc ctcagccagc
    63061 cggcgttcat caatttcaat gccggagcag ccttcggtaa atataaagct tcctttttgt
    63121 ggatcaaaat aatccagacc gctgttttta ggtgcagttt cccacttttt tgcaaaggtt
    63181 tctgcctgga ttaaaatttc cttttttaat tcttcgttta tcaggtctgc aggggtacgg
    63241 gtcagatctc cggactggga ggaacggcag agtgttccaa tccattcctt cagccgggac
    63301 gataaaggat tttccacagc cagctgttct tctccatatt ctgctttcat gtcccaaaga
    63361 aaaagctggt ttaaggcttc aaagctctca tccgggctta agccgttcag agaaatgccg
    63421 ttgaccgtta ccgcaggcgc ggtaagcgag gcatctgttt cagaaagcga ggtttcatgg
    63481 ctgcccgcag ccggtttccc tttaaaatgg gaaatagtga cggctgagac agcggctaaa
    63541 agaaagattc ccgctccggc ggcagccagg ggaagaagga attttccctt ctccgtttca
    63601 gatgcaggcc ggcggctttt tctggggccg cggtaggaag agcgggaggc tttgtttcgt
    63661 ctgtgatcgt aatggtttgt cattctaaac tccggatata catagttttg atacctggct
    63721 gattttcaat ccggtatcat attattcata tgtgaatact gggaattatt atataggtac
    63781 cttaaaggaa agtcaagagc cgggagaagg cagtaattgc catgcacagt ataacgccgc
    63841 aggctgtggg aagaagaacg gccagacagg tccattttaa gcttccggtt tcctttttta
    63901 tcgtcataag ggtagtagaa cagggccagt gaaacaggaa aaataccatg gtgcaaaggg
    63961 cagtgaccca ggtccagccc tgagagataa ggagcgtatg aagttcggaa agatttcctg
    64021 gttcgatcag ggttccctga gaggtatagg ccataataat gatggggatc acgatttcat
    64081 tggccggaag cccaaggata aatgccataa ggattacccc atccatcccc atcaggcgcc
    64141 cgatcggatc cagatatccg ctaaagcgtg ccaaaaggct gatatctcca atggaaacat
    64201 tggctaaaat ccagatcaca agcccggcag gagccgctac cgcggcggca cggcccagaa
    64261 taaaaagggt gcggtccaaa atggagcgcc agattaccct tccaatctga ggtcggcggt
    64321 agggcggaag ctccagagta aaagaggaag gcataccttt aaggaccgtg gcagacagaa
    64381 ggcgggatac aaaaaaggtg gcggcaacac cggtaaggat aaaaaaagta aggatcaggg
    64441 caggacggag agaggaaata ggacctgcgg acgaagctcc tgtaaaaaac agcgtgatca
    64501 gagttacgag ggccggaaga cggccgttgc aggggacgaa gctgttggta agaatggcga
    64561 tgagccgttc tctgggcgag tcgatgatcc ggcagcccgt tactccggct gcattgcagc
    64621 caaagcccat agccatggta agggcctgtt ttccgcaggc tttgcagcat tggaagggac
    64681 ggtccagatt atatgcgatc cggggcagat aaccaaaatc ttccaaaagg gtaaagagag
    64741 ggaagaaaat ggccatgggc ggaagcatga cagagataac ccaggccagt acccggtata
    64801 cacccagaac caaaatcccg tggagccatt ggggcgcatt gactgccata aaagcagcgg
    64861 taagtttttc ctgaaaggag aaaaagaggg aggaaagcag ggcggaaggg tagttggctc
    64921 ctgtgatcgt cagccagaaa atgagcgcca aaagaccaat catagccgga tatccggcca
    64981 gccgcccggt caggatgcga tcccaaaaag catccctttt gccgtagggt ttttcaggaa
    65041 gggagaccgt gcgggaagca atggactccg cagtatttac gatgccggat acgattcggt
    65101 cctttaaatc atcctcaaag atcccagcct gatccaggtg tgaccgggcc tggaggacag
    65161 cctgacataa cgcatcatcc tcggacagat ctctgcctaa aaaggtattt aaagagcgaa
    65221 gcaggccggg atcattttca aggagtctta aggccagcca gcggggagaa aggcggctgc
    65281 tgagtggttc tgtaaagggc agaagcatgg aaatggcctg ttctacagct gcgtcatagg
    65341 aaatatgaaa tggctctgtg tctggatgat cgaccgtttc atccagccga tctaaaaaat
    65401 gttccagaga gcgctttttt cttgcagttg ttccagcgac aggagcagaa agttcccggc
    65461 tgagagccgg cagatcaatg cgtatgtttt ttttcgctgc ttcgtccatg aaattgacgc
    65521 agaccatggc gggaattccg gtttccagaa tctgcaggac aaggtttaag ttccgttcca
    65581 gacaggtaga gtcacagata accacagcgg catcacagtt cccaaaacag atgccgtccc
    65641 gtgcaagcgc ttcttcagca gagtgagcca taagagaata cgttcccgga aggtctgcga
    65701 aaacatagtt gtattttttt gaggagcagc ttcctttaaa acaggaaact gtaaccccgg
    65761 cccagttgcc cgtgtgttgg gagctgccgg taaggctgtt gaaaagggtg ctttttccta
    65821 cattggggtt gccggacaga gccactgaaa attggttctt attttcgttt ccatccaccc
    65881 ggatctggcg gctgtattca cgccgtatgg caatgactgc accccggatc cggtaggaga
    65941 ccgggtcacc taaaggactt acgcccaggc attctacctg tgtgtctgca gtcagcccca
    66001 gatccataag cctcctgcgc atggctccgg aaacaagaag ttccgttacc cgggcggtct
    66061 gccctggttg tatatcattt aaggaaagga aaggattcac gatccgtata cctgcccgtg
    66121 tttgttctta ttattacagt atggttcagg cgggaaaatg tgactggaaa taaccggtga
    66181 agtggtgtat aataagaaga aaaaatgaca acaggagagt tttgctatgg aagaaagtta
    66241 cattgaatgg ctggtaaagc gcaaggatcc tatctatgcc cttccggtaa agatcattat
    66301 ggtgcttgtc tgcgcctttt ccgtactgct ggcgctgcag acggtgatcg gagttgtgat
    66361 catgacagca gcaggtatcg gtacctattt tattttcctg aatttaagtg tggaatatga
    66421 gtatctgttt gctgagggcg gtctgcggat tgaccggatt ctgggaaagg ccagacggaa
    66481 aaaggccttt gactgtgaaa aggatgatgt acagctgatc gctccggtgg attcctatat
    66541 gctgaaggat tatgccaagc aggggatgaa ggttatggac tgtacctccg gaaagcaggg
    66601 tgctgaagtt tatgcgctga tgtatcagaa gggagccgac accatgaaag ttcttattga
    66661 gcccaatgaa aagatgctgg cagccatgcg ccatacattt ccgcgtaaat tcgtgaagta
    66721 agagaaacaa aatattaggg tcactgatgg aatcctatta gtaaattcaa tgaatcataa
    66781 cgggaaaatg ggtttgacaa tcctaataaa atttgctagg attagatact aataagatgc
    66841 tgagggcagt ataccacaca gaaagcgagg aagttttaat gggtacaatt atcggcgaag
    66901 gcattacctt tgatgatgta cttctggttc cgtcttattc agaagtgatt ccgaatcagg
    66961 tagatttaac cacctatctt acaaagaaga tcaaacttaa cattcccctg atgagtgcag
    67021 gcatggacac cgtgaccgag caccgtatgg ccattgccat ggcgcgccag ggcggtatcg
    67081 gtattatcca caaaaacatg tctattcagg aacaggcaga agaagtagac cgtgtaaagc
    67141 gttccgaaaa cggtgttatc acagatccct tctatctttc ccccgaacat actttaaagg
    67201 atgccaatga ccttatggca aaattccgta tttccggtgt tcctattacg gagggaaaga
    67261 agctggtggg tatcattacc aaccgtgacc tgaagtttga ggaggatttc agccgtccga
    67321 tcaaggaatg tatgacttcc gagaatcttg tcactgcaaa ggaaggagta acgctcaagg
    67381 aggcaaaggc gatccttgct aaagccagag tggaaaagct tcctatcgta gatgacaatt
    67441 tcaatttaaa gggtcttatc acgattaagg atattgaaaa gcagatcaag taccctctgt
    67501 ctgcaaaaga cgagcagggc cgtctgctct gcggagccgg cgtaggcatt actgctaatg
    67561 tattagagcg tgtagctgct ctggtagaag caaaggtaga tgtaattgtt cttgactctg
    67621 cccatggtca ttctgccaac gtgatccgct gtgtgaagat gatcaaggat gcatttccgg
    67681 atcttcaggt agtggcagga aatgtggcta ccggtgaggc gaccagagct ttgatcgagg
    67741 caggtgcgga ctgtgtaaag gtaggtatcg gccccggttc catttgtacg acccgtgtgg
    67801 tggcaggtat cggcgtacct cagatcacag ccgttatgga ctgttacaat gtggcaaagg
    67861 aatacggcat cccgattatc gctgacggcg gcatcaagta ttcgggtgat gttacaaagg
    67921 cgatcgcagc aggcggcagc gtgtgcatga tgggaagcat gtttgcaggc tgcgatgaga
    67981 gtccgggtac ctttgaactg taccagggac gtaagtacaa ggtataccgc ggcatgggat
    68041 ccatcgctgc catggagaac ggaagcaagg accgctattt ccagaccgac gccaagaagc
    68101 tggtgccgga gggtgtagaa ggccgtgtgg catacaaggg tatggtagag gataccgtat
    68161 tccagctgtt aggcggttta agagccggta tgggctactg cggagccaag gatatcccca
    68221 ctctgcagaa tacggcaaga ttcgtgaaga tctctgccgc ttccttaaag gaaagccatc
    68281 ctcacgatat tcacatcaca aaagaagctc ccaactactc gattgacgag taagaggggg
    68341 agaaaagaaa gcccggagaa agcgcgatat ctttcttcgg gttttattta tgaaataaaa
    68401 gatgggatcg gtgaaaagaa gcattcaggt gtattcttga ctttcccgcc ccccaatgct
    68461 agaataaata ccatggataa gaagagaata gcagtttgta ttacaggaat tttgatcata
    68521 gcggcctgtc tgacggggtg ccaaaagcat agggaacctg tcagcaggtc ttcttttttg
    68581 ctcaacacat ttattacggt aacgctttac gattccagag aggaagagat cctggatggg
    68641 gtgacggagc tttgctcaga atatgaaaag aagttcagca ccaccataga aaccagtgaa
    68701 atttatgcaa tgaaccacag aaagccggga gaacgggagt ttactgtgtc acaggatacg
    68761 gcggagctga tagaaaaagg actggagtac agccggctgt cagatggggc ctttgatatc
    68821 accatagaac ccttaagccg tctgtgggat ttttcgggct cagatcctca tgtaccgtca
    68881 gaggaagcaa tccggaaaga acttttaaag gtaggatatg aaagagttgc ggtaaacggc
    68941 aatacagtgg tttttgcaga tgatctggta tctattgatc tgggagcgat cgccaaaggg
    69001 tatattgcag accgcatgaa agaatatctt aaagagcagg gggtagagag cgccattatc
    69061 aatctgggag gcaatgtcct ttgtctgggg gaacggcccg gaaataagcc atttaaaatc
    69121 ggtcttcaaa agccctttgc agagcggtcc gagacagtgg cggctttgga tatccgtgat
    69181 atgtctgtag tttcttccgg ggtatatgag cgccattttg aggaaaatgg agtcaattac
    69241 catcatattt tgaatccatc taccggatgg ccctatgaaa atggccttac tcaggtcacg
    69301 atcctgtctg agcagtccgt agatggtgat ggactgagca cggcctgttt tgcgctggga
    69361 ttgaaagaag gaatggagct tgcacagtcc ttagagggcg tgtatgcggt atttgttaca
    69421 gaggatggga cgctccatta ttctaaggga gcagaggcct ttatcagcag tccataaata
    69481 tagaaactta actttacatt ttttgcaggc tgccatataa tgagaacgtg tttacataca
    69541 gaaatacgag gaataaaatg tttgaagcaa tatggaaaaa atgtgtaaat tatgaaacga
    69601 tcagttatct gatctgcggg atcctgacta cggcagtaga ttttgtgtcc tatgccatgt
    69661 tccgccgtat ggatatggga gtgggaatgg cacaggcttt ttcctggggg gcagctgtgc
    69721 tgtttgccta tgtggtgaat aagctgatcg tatttaaaaa ttatgatctc aggccatcct
    69781 atcttatgaa ggaggcaggc gcttttttga tgacaagagg aatttcaggc gtggctgtct
    69841 ggctgctgat ggttgttatg gtagcgcttg gagagggaag aggcttttta tatgagatgt
    69901 tctgcaagat ggtctcttct gcggtgaacc tggtggcaaa ttatattttc agcaagctgt
    69961 ggatttttaa aaaaaggtca gagagcaagg ggaaacagca gtgaaacgag aagtgatcgt
    70021 tcaggcagaa cgggaactgg atgcggtatt ggagctgatc caggagtcgg aggggatttc
    70081 aggaagggat gacataaaag aggagcgtat gaaagaaaga aatcggattc aggaagcaga
    70141 ggtagaagaa agcctgaccc gtattaatat ggaagacacg gcagatatgc tgcagtatga
    70201 agcgtgccgg gaattaaaac gctgccggga cgttcatatc ctgcgtccgg gtgatagcct
    70261 tctggctgct ttttttgttc cggtgcttgt tttggtggtt atctttgcac agagaggtat
    70321 tttccctttt ggggaagaat gttttctgcg tacggatatg taccatcagt atgcgccgtt
    70381 tttctctgaa tttcagtata agctgactca cggaggaagt ctgctttaca gctgggatat
    70441 cggtatgggg gtaaatttct cagcattgta tgcctattat ctggcaagtc ccatgaactg
    70501 gcttgtggct ctttgcccca ggcagttttt gattgagttc atgacggttt tgatcgtcat
    70561 aaaaacaggc ctgtccggtt tgttttttac ctggtacctt agaaaacata cggggaccag
    70621 ggagtttggt tcctgctttt tcggcatttt ttatgcatta agcggctata tggcagccta
    70681 cagctggaat atcatgtggc tggactgtat ccttttattt ccggtcattc ttttcgggct
    70741 ggaacggctg gtaaaggaaa aaaaggggat ggtttactgt attgccctgg ggctttcaat
    70801 cctgtcaaac tattatatct ccatcatgat ctgtattttt atggtgatct atgcagcggt
    70861 tctggtgatt ttatatcctc cgaagaaggg aaaggagttc gcagccacag ccggcagatt
    70921 tacgctgtat tcccttttgg ccggcggact ggcggcagtg gttctgctgc cggaaattta
    70981 tgcccttcag gccaccgctt caggaaattt tgactttcca aagacagtca gttcctattt
    71041 ttccatattt gacatgatcg cccgccatat tgggaacgta cagacagaga tcggactgga
    71101 tcattggcct aatatttact gtggggtagc tgttttgatg ctcctgctcc tgtatcttgg
    71161 gaataagaac attaaaataa aggaaaaagc agtatatttc agtcttctgc tctttttcta
    71221 cgcaagtttt tctgttaatg tactgaattt tatctggcat ggctttcatt atcccaacag
    71281 ccttccctgc cgtcaatcct tcatctatat cgcgctggtg ctggtgatgt gctataaggc
    71341 gtatttggag cttaaaaata caccctggaa gcatatcgtt atggcctttt ggggagctgc
    71401 agcctttgtg atcctggctg aaaagctggt agataatgag gaacagttcc attttgccgt
    71461 attttatgca gctatcctgt ttctggctct gtatacgggc tgtatttatc tgtatcacag
    71521 ccggaaatgg tgtcgggatg gggttctttt agcagtgctg ggactggtat tttgtgagtc
    71581 ggcagtcaat atggcggtaa cgagtattcc cacaaccagc cgtacagcct atgtaaaaga
    71641 taatgaagat accatgcttt tggcagacag catccgctcc tcggtatttt accggattga
    71701 aaagggtgaa agccggacca aaaatgatgg ggcatggatg aatttccctt ctgcctccct
    71761 gttttcttcc gtagccagcg ccgctatgtc ggactttttt aaaagcgtag gctgtgagag
    71821 ctccaccaat gcgtacagtg taaaaggaag tacgcccttt attgacgctc ttttcgctac
    71881 ccgttacggc atctatccgg atcagcagcc tgcggacggc ttaaaggagc agattggccg
    71941 tcagggctcc atgtggtttt atgaaaataa atataccctt ccggtggggt ttgtgatgcc
    72001 tcaggatatg gaaaccaact gggttcttga ttcgggaaat ccggcaaatg tgcagaatga
    72061 tctttcttcc gtactgggag taagcaatct gcttgtgccg gcagagggag tatcggaagg
    72121 aaagaagttt acctttacag cagatgcctc cggcgattat tatgtctatg tgaccaataa
    72181 aaaggtagaa gaggtttccg ccgaaatagg ggagagaagt ctttcctttg acaatgttga
    72241 caggggctat tttctggagc tgggctatct gccaaaggga caggaggtaa tccttcagtc
    72301 caaaacagat ggaaatcctg ccatgcaggc ggaaatctgg cgttttgatc cggaggccat
    72361 ggaggagatt tatcagtgtc tgagcaaatc gcctcttgaa ctttccagct ggacggatac
    72421 cggccttgca ggaagcataa atacgccgga gggaggaacc atgtttacca gcattcccta
    72481 tgataaggga tggaaaatct gggtagacgg gacggctgtt tcagggcgtc cggtgtttga
    72541 tgcatttctg ggcgtggatc tggaacccgg cgagcataag atccggctgt cctatgagcc
    72601 tcaggggcta aagacaggtg ccgttattac aggcgtaagt gcggccgctg tggcagcact
    72661 ggctgtctgt ggctggatga aaaataaaaa gaaatttcta aaataaatca tatataaagg
    72721 agactactat tatgaagctt tggggcggac gttttactaa ggaaacggat cagctggtac
    72781 agaattttaa cgaatctctt tcatttgatc agaaatttta ccgtcaggat atcagaggaa
    72841 gcattgctca tgtaaagatg ctggcaaagc agggaatcct ttctgagaag gacagagacg
    72901 atattataaa gggactggaa gggatccggc aggatctgga cgagggcact ttgatcattc
    72961 cggctggttc cggttatgag gatatccatt cgtttgtaga gggaactctt acagagagga
    73021 tcggagaggc aggaaagcgc cttcatacag gccgcagccg caacgatcag gtcgcccttg
    73081 acatgaagct gtatacaagg gatgaaattg acgagcttga cggtctggta aagaagctct
    73141 tagaggaact ccttgtgatc atggaggcaa atacagaaac ctttatgccg ggctttaccc
    73201 atttgcagaa ggcacagccc attacgctgg ctcaccatat gggagcttat tttgagatgt
    73261 tttcaagaga ccgctcccgt ctggccgata tccgcagacg gatgaattac tgtcctttag
    73321 gctccggcgc tctggcaggc acgacctatc ctctagatcg gtcttatacg gcagagcttc
    73381 tggaatttgc agggcctacc ttaaattcca tggattccgt atcagaccgg gattatctta
    73441 ttgagctttt aagcgccctg tctgttattt ccatgcattt aagccgtttc tgtgaggaaa
    73501 ttattatctg gaatacaaat gagtaccggt ttgtggagat cgacgattcc tacagcaccg
    73561 gaagcagcat tatgccccag aagaagaacc ccgatattgc cgagctgatc cgcggaaaga
    73621 gcggccgtgt ctatggagct ctgatgtcca tgctgacaac tatgaaggga attcctctgg
    73681 cttataataa agatatgcag gaggataagg agcttacctt tgatgctatt gatacggtaa
    73741 aaggctgtct tgccctgttt acagggatga tctcctctat gaccttcaga aaagatgtaa
    73801 tggaagccag cgcaaagaga ggctttacaa atgcgacaga tgctgccgat tatctggtaa
    73861 atcacggcgt tcccttccga gatgcccatg gtattgtagg acagctggtg cttttatgta
    73921 ttgaaaaagg cattgcgctg gatgatcttt ccctggagga atataaggag atcagtcccg
    73981 tatttgagga agatatttat gaagcaatca gtatgaagac ctgtgtcgag aagcgcatga
    74041 ccattggtgc tcccgggcct gaggcaatga agcaggtaat tgctgtttat aaaaagcagc
    74101 tgcagtaacg gcaggtaatt gattgcatac agaggatgaa atatatgaaa aaaattgggg
    74161 gggggtggaa catggaaacc attagaaaga gaagccgtat ttttaccatc tgcgcggtgt
    74221 tggttgctat gttctgtctg gcagtaggtg ctggaaatac cgtatccttt gaacggatga
    74281 tgaaccaggt ggtagagcgg tatgtgtcag aaaacaatca gcaggttgca agccagattt
    74341 cataccgtct gaaaatggga agagagtttg tatcggactt tgccgatacc ctgagccgaa
    74401 tgccccaatt tcttctcaca gaggatttta tatccagaaa gacagccgcc gtggagttgg
    74461 aaggacttat ggttcttttt gaagacggca gtgtgtttcc cgaatctgcc tgtccggatt
    74521 atctgagcca gtggtttgag aaaaatccgg acatatggga aaaacaggta acctcctatg
    74581 tagcagatca gaatcttatc tttactgctc ctgtacatta tggggatgga acagaagcgg
    74641 ttgtagtagg acttcagaac tactggagca tcaaggctct ggtcttccgg gcagattatc
    74701 aggagcaggg agccagcttt ctcattgaca gaaaagatgg agagatcctg atcgcagatg
    74761 aaaagtcagc tttatcagtt acagatcagc agctctatgc tcttctagag acaggaaggg
    74821 agctggaagg agacaggcag gtctatgaag cggtgatgcc gaacggacag gaggtttttg
    74881 tttccttgta tgaggtaagg gatacggact gggtacaggc agccgtgatc ccgtccgatt
    74941 ttctgatggc ccagatcgaa cgtcatatgg atgtttatgc cgcggtagta gtggctgtca
    75001 tggttttgct gggcgtattg atcggacatc tgatgaagga aaaccagagg tgggagaggc
    75061 tttctgttac agagcctctt accggaggct ggagccgcga aggctttata aagctgggta
    75121 cgcaaaatac ggaaggacac aggcttcacg aatggatcgt tgtgtatctg aatattaaag
    75181 atttccgaca tattaacgaa agctggggcg aagaagatgg gaatctgatg cttcagttta
    75241 tctaccgtat gctttccgga cttataaatg aagaggagct ggtgagcagg agcagcatga
    75301 atcatttctt cctgttatta aaggaagagg acgatcggga ggtcaaccgt cgaatcctca
    75361 aaatgaaaga agcagtaaac gagcggatcc gttgtaaatt cagcgagtac agcattgatt
    75421 ttgcggttgg cgcctgccgt ctggatattg caggaggagt tcctacagcg atgaataagg
    75481 caatttatgc cagcaagctg tcggatacag acaatgtatg cagcttttac aatgcaggta
    75541 ttgcaaatat gcttgaaagg gaggagcggc tgaatactct ttttgaggat tctgtaaaaa
    75601 acagggattt caaggtttat cttcagccga aagtgtctcc ttcagaaacc agagcctgtc
    75661 aggcagaggc ccttgtaaga tggtttcatc cccaggaagg ggtgatctat ccctctgaat
    75721 ttattccgat ttttgagaaa aacgggaaaa tctgctccct ggatctttat atgtttgaag
    75781 aggtctgccg cctgatcgat cgctggatca aggagaaaaa ggcactggtt tccatatcgg
    75841 tcaatgtgtc acgcttccac ttaagagagg ctggcagcga gatatggaag gagtacaaac
    75901 ggatccttga ccggtatgat attcctgagg gcgtgataga aatggaactt acggaaacta
    75961 ttcttttgga ggaaaatcag attccttttg taaaagccgt cttaaacggt ttccggtcct
    76021 gcggacttcg tgttgcattg gatgattttg gctttgctta ttcatccctt tccatgttaa
    76081 aagagtttgc agttgataca ctaaagatgg atcgcagttt ttttgtcaac gaaaatgaca
    76141 aatcaagaag aattgtagga agcattattc agctggcaca cagcctggat atggatgtgg
    76201 tagctgaagg aattgaggca aaggaacagg tggaaatgct gaggaagatg ggctgcgact
    76261 tgattcaggg ttatgtctat tctaagcctc tgcctgttgg ggaatttgag cagtggagag
    76321 aggagtatga caggtaacag tataaaaaag actttgcccg gaatggcaag gtttatgata
    76381 ctgattttaa ttattttgat ccttccgacg aatgtggcat tacagttata tatacagcat
    76441 cagaaccaga agcagaattc agaagagata ttctggcagc tggaacagct tatacggaac
    76501 aatatatatg atctggagcg ggagaaaagg gacttttctc aaaaatgtat tcagtcagct
    76561 aaaatggctg cctactttgt ggaacataat ccggggtata ttacagatct ggagcatacg
    76621 aaggagctgg ctgaaaagct ggatgtagat gagatccatt ttattgatcc tcagggggag
    76681 atttatgcgg gtacccatcc caggtattat ggttttacat tccgggatgg agaacagatg
    76741 cagttttttc tttccatgct ggaagatact tctctggagt tgtgtcggga gatcactccc
    76801 aatactgcag aaggaaagat gatggagtat gcggcagtat ggatggaaga taaaagcgga
    76861 attgtccaga tcgggatgga acccaaacgc cttttaaagc agatggagga aaaaagcctg
    76921 aaaaaggtgg tttctgagat accttccggt cttatgggat atctgcatat aatcaataca
    76981 aacacaggga agatcgaagc ttcgactgtc cctggaatga atgggctgga tattcatgaa
    77041 gaacttaaaa cggaagataa aataaaagct cctgcttccg gccactatcg gtataacggc
    77101 cggaaattct gtgtctatac aaaatattac ggtcaatatc tcctggtgcg ttcttttgtg
    77161 tcagcttatc cgctgcgcag cgcggtgcag tctactctgc ttgtatttct ctatatactg
    77221 ggggcgtctg tggccgttct gtggattatc atgtggtaca tgaaagtaaa acttgcaggg
    77281 aatctgactg cgattgtagg tgaattgaaa aaaatagaag ggggagatct gaccaatctt
    77341 accatccaca cgggaatttt agaatttgac gagctggttc agtatatcaa tgctctttta
    77401 aagagtatcc gtttaaactg ggataagatg gatcatgtga ttgaaaacag ccgtgttccg
    77461 gtgggagtat ttgaatataa ccatttttat aaaaaaacct ttatgaacaa ccgtattctg
    77521 aaaattcttg ggatttctga tgcagatgaa aataatgatg ctttgagcaa caggataagg
    77581 aaaaagctgg aagaagcgga gaaccatcct gtggatgcag gaaaatctct ttatgaatat
    77641 gacagaaacg gggaaacggt ttttgccaga attgaaaaat ttacagatga acagagtacg
    77701 acctggtgcg ttacggacgt ttcccagtgg tggaatgaga tcaatcgctt aagagaacag
    77761 agcagcagag acagcctgac gctgctttat aaccgccggg gctttacgga acggctggag
    77821 gctctgtttg ccgatcccgg agctattgga agcggagtga tgatcatgct ggatgcagat
    77881 ggactaaagg ggatcaatga catgtacgga catcatatgg gagatgttta tttacgtcaa
    77941 attggccgtc agattgaatt ttcgggagag aaacgtttta tcagcgccag aatgggaggc
    78001 gatgaatttt cggcatttct ttatggttac gcttcaaagg aggaggcaga gaaggttctc
    78061 cgggactttg aggctcatcg cggcgaggtt tttatggatc ccgagatgaa acatattttg
    78121 ggaactgtag aattttccgt tggatatgcg ttttatccgg aggacggaac ggattaccat
    78181 gtgctgatgc atatttcgga tgaacggatg tatgaagaga aaaagaaacg taaaggcaga
    78241 aggagcagag aagaatgaaa ctgtttcatc tttcagatct tcatcttgga aaacgggtaa
    78301 atgagttttc catgctggag gatcagcagt atattttgag ccgtattctg gaatgggttc
    78361 ataaggagaa gccggatgta ctgatcctgg cgggtgatat ctacgacaaa gcagtaccgt
    78421 ctgcagaggc ggtcagcctg tttgactggt ttcttacaga gcttgcagac agagcgtgtc
    78481 cggtgtgtat tgtaagcgga aaccacgatt cggcagagcg gctggcattc ggcgccaggc
    78541 ttatgaatgc cagaaacatt tttctgtccc cggtttatga ggggaaggtg gagacggttg
    78601 actttaagga ttcctttgga aggatccgga tccaccttct gccctttgtg cgtcctttat
    78661 cggtacgcca tgcatttccc aaggaggcgg aagggatcgt tgattatcag acagctttaa
    78721 ggactgcagt ggagcatatg gagctgctag aaggagaacg gaatgtgctt gcagctcatc
    78781 agtttatcac aggggctatg agctgtgaat cagaagaagt gactgtaggg gggctggaca
    78841 atggggacgc ctccctcttt gatgcgtttg actatgtggc cttaggccat atccacagcc
    78901 ctcagagtgt ggggcgcaaa gaggtgcgct actgcggtac gcctcttaag tattcctttt
    78961 ctgaagtatc ccaggagaaa tccattaccg tggttgaatt ggaagaaaaa ggaaatgtgc
    79021 agataaaaac tcttcctctc ataccgctgc gtgatatgag aaagatacgc ggtacgtata
    79081 tggaggtaac ggatcgggct ttttatgagg gaaccgatca gcaggattat atccaggtga
    79141 ctcttacaga tgaggaggat ataccggatg gtctccagaa gctgcgtacc atttacccta
    79201 atattatgcg cctggaatac gataaccgcc gtaccagaaa agacgcagag ataagagaat
    79261 gggaaaagca ggaagagctt tcggagctgg agttgttttc gcggttttat gagcagcaga
    79321 acaatcagcc catgagccca gagcaggaag cgtttgtcaa acatattatg gaagagataa
    79381 aggaatagag gatgaaaccg gaaaagctga tcataagcgc ctttgggcct tatgcggagg
    79441 aaattgaaat tgattttgaa aagctgggag acaggggcct gtttttgatc acaggagata
    79501 cgggggccgg gaaaacgacg attttcgatg ccatttcctt tgccttgtac ggagaagcca
    79561 gcggagagat cagagaagcg ggaatgtttc gaagcaaata tgcatcagag cagacgccca
    79621 catttgcaga gctgactttt tctattcagg aaaagagata ccgtgtccga cgtaatccgg
    79681 agtatctgcg tccaaaaggg agagggaccg gctttactgt tcagaaaagt gatgcggaac
    79741 tgatcttttt tgacggccgt ccgccaatcg taaagaataa ggaggtcaca aaggctgtca
    79801 cagaactttt ggggctggac taccgccagt tcactcagat tgccatgatc gcccagggag
    79861 attttcaaaa gcttctctta gccggaaccg cagagcggtc agagattttc aggcgtattt
    79921 ttcatacaga gccttgtcag gaacttcaaa atcgtttgaa atatgcagta aaagaacgct
    79981 ggaaggacta tgatgagatg cggcgcagta tcagtcagta tctggacgga gtacagtgcg
    80041 gatgtgcgcc ggaaattgac gcagaattta agactctgaa aaaagaacat tttgacggaa
    80101 aagccgtccg cggcatggaa cttttgagcc ggcttctgga gatggataag aacagcctgt
    80161 ttcagctgga taaggagatc agaggactgg aggaagaaat ccaggaacag gatcagcttc
    80221 tgggaaaagc agaacagagg cgcaggctgg tacaggagtt aaagcttagg gaaacagaag
    80281 aaagggaact ggctccggtt ctggag
//
LOCUS       DBMDUH010000002        75904 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_395_length_75904_cov_7.373165, whole genome shotgun sequence.
ACCESSION   DBMDUH010000002 DBMDUH010000000
VERSION     DBMDUH010000002.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 75904)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 75904)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75904
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_395_length_75904_cov_7.373165"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="ACZPCG_00335"
     CDS             <1..834
                     /locus_tag="ACZPCG_00335"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006772029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HIR1283553.1"
                     /translation="AYGQDTVIAVKQFQARNDQVVDGYLGPSTRIALNDPSARPNGLM
                     LGEQGETVTRVQQLLSKYGYLSSANVTGYYGEVTEQAVKNFQSRNGLAADGQVGIQTM
                     TKLTSDNVRRPAANSGGGSSAQTGGGSTGGSGGNKKPAGNNGSQGGSSSAPKAPSGTG
                     VSALISVASSKIGSPYVWGAKGSNAFDCSGFVYWCLNQVGVRQSYLTSSGWRNVGRYT
                     KITNFNDIQAGDIVVVNGHVGIAAGGGTVIDASSSNGRVVHRSLSQWWRNNFICAWRI
                     F"
     gene            942..1637
                     /locus_tag="ACZPCG_00340"
     CDS             942..1637
                     /locus_tag="ACZPCG_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1283554.1"
                     /translation="MQTILVCDDDKQIVEAIDIYLTGEGYKVIKAYDGYEALEYLENN
                     SADLLILDVMMPGLDGIRTTLKVRENSSIPIIILSAKSEDADKILGLNIGADDYITKP
                     FNPLELVARVKSQLRRYTQLGNLNQQEQDQVYKCGGLQINDDNKEVTVDGEPIKLTPI
                     EYNILLLLVKNAGKVFSIDEIYEQIWNEEAIGADNTVAVHIRHIREKIEINPREPKYL
                     KVVWGVGYKIEKQ"
     gene            1646..4003
                     /locus_tag="ACZPCG_00345"
     CDS             1646..4003
                     /locus_tag="ACZPCG_00345"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1283555.1"
                     /translation="MEQNRKKIRIQKVVLSLLSVIFLAMALAGISAMYLNGGYGRGIS
                     WVYEETYEDSDTFSMKLGKDIENIFTYIGYKDLFETNGQLDMSKPIVRLTDGPGVDED
                     WTLDQIVRYARIRGYYLGENFTVQGSPMSMDDDDMEVTVQFLNTGFDFLSMGEMETRM
                     TKEELALDILECLGQYYSIYYNFMEHQTNVRFRVVYKSDEGVETVYTNVPELSLDELR
                     GFGKYLFIPGNTIRMDTNLAVIPQNAAALLETWNPNNNDQNYLVVSVDTTYPYVDSYS
                     EESAQYRSVRNAFIMGMGGVIVGFIGLFAALVWLVFLSGHTDNLDSEIRLYPVDRIPT
                     EVCILLGAVAAAVLLLWGKRLGMMVIGLFFSEEHLPFWNKMLRYLIIYSCAVPCGFSL
                     LRRYKAGTLWTGSLGKRGTAAFKAYREKTSYAVGGALCYLTFLGCNGFMMWLILFLFT
                     TRGERISYRIMYYVVLVLFVWLDGWVFHRLFREACQRDLLDQAISTISTGDTGYRLDT
                     SMLTGKEKKMGEHINNITSGLDTALQEKVKSERLKADLITNVSHDIKTPLTSIINYVD
                     LLKRQKIQDPKIAAYLEVLDQKSQRLKTLTEDLVEASKASSGNLKLEVSDINLVELVQ
                     QTNGEFEERFALRRLELIQTLPEEIIMIRADGRRLWRVLENLYTNAFKYAQEGSRVYV
                     DVREEGDKAVFTIKNISEKPLNISPDELTERFVRGDVARTTEGSGLGLSIARSLTQLQ
                     KGEFIISIDGDLFKAQVTFPVERRERKAERIAEEIPSDSSEWEDT"
     gene            complement(4083..4832)
                     /locus_tag="ACZPCG_00350"
     CDS             complement(4083..4832)
                     /locus_tag="ACZPCG_00350"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008720753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HIR1283556.1"
                     /translation="MARELVFTSEKKTVYRDGSTAIKEFCDGFPKAEVLNEALCNARV
                     EDIEALNVPKILGVSVVDGKWSILKEYVEGKTLQQLMDENPDKLEEYMEGMVDLHLLI
                     HKQACPLLNKLKEKTIRALKSVEQLDDSTRYELMTRLDGMPKHTKLCHGDFNPTNIIV
                     GDDGKWYVIDWVHASQGNASADVARTYLLLSLKDRKRAELYMDLFCEKTGTEKRYVQG
                     WLPVVAAAQLAYKRPEEKELLESWINVFEYQ"
     gene            complement(4988..6955)
                     /gene="glgB"
                     /locus_tag="ACZPCG_00355"
     CDS             complement(4988..6955)
                     /gene="glgB"
                     /locus_tag="ACZPCG_00355"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HIR1283557.1"
                     /translation="MAARKSTPAKPTAAAKKTGTSSRASAPAGTGMISELDCYLFGAG
                     THYDIYQKLGAHPMTFKGKSGIYFAVWAPHAEQVHLVGDFNGWNPDANPMKKISDMGI
                     WEYFNPGMKTGELYKFAITTDTGKILYKADPFAFSAEYRPGTASVTADLSGFSWADTD
                     WITKRAQKDPEKQPMSIYEVHLGSWRKKNRPEKDGCYTYIEAAHELAAYVKEMGYTHV
                     ELMGIAEHPYDGSWGYQVTGYFAPTSRYGSPKDFMYFVNYLHKNGIGVILDWVPAHFP
                     KDAHGLADFDGHPLYEYEDPRKGEHPDWGTKVFDYGKNEVKDFLISNALYWVEQYHVD
                     GLRVDAVASMLYLDYGRQDGQWVPNIYGGNQNLEAIEFFKHLNTVVQGRNLGVLMIAE
                     ESTAWPNVTKHPEQDGLGFTFKWNMGLMHDFLEYMKLDPYFRKYNHNRMTFSLTYFNS
                     ENYILVLSHDEVVHLKCSMINKMPGLGEDKFSNLKAGYTFMLGHPGKKLLFMGQDFGQ
                     WHEWDEKVALDWHLADEDSHRKLQEYVRDLLHIYKKYPCLYRLDNDWNGFEWVNANDG
                     DRSIFSFIRRDETGKKNLLFVINFTPVARPDYRVGVPKKCKYTLLLDNEHGAYKASQC
                     PSYTSVKGECDGKPYSVAYPLPAYGTAIFRF"
     gene            complement(6957..8297)
                     /locus_tag="ACZPCG_00360"
     CDS             complement(6957..8297)
                     /locus_tag="ACZPCG_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HIR1283558.1"
                     /translation="MSTWYEKAVFYHMYPLGMTGAPKQNTGTEQTDRFDELELWIPHI
                     KALGCNAIYIGPLFESTSHGYDTRNYKQTDRRLGSNEDFRHFISLCHENGIRVVVDGV
                     FNHTGREFFAFKDIQEKKWDSPYKDWYKGVNFDWSSPLGDPFGYEAWQGHFELPCLNH
                     ANPQVCRYLFDVIRFWVDTFDIDGIRLDCANVLDFHFMEEMRRETKTMKEDFWLMGEV
                     IHGDYGRWVNDHMLHSVTNYELHKSIYSGFNDHNFFEIAHNVRRLEAIGRSLYTFVDN
                     HDEDRIASKLRTKEHLLPVYTCLMTLPGIPSIYYGGEWGIEGKRTPSCDDALRPAISP
                     DAFDSLRCTLTDRISKLSIIHQENEELHMGRYQELLLTNRQYAFARHGEHSLIITALN
                     NDDNEAVLNIPVPMAASQAVNLLEDGEILPIRDGKLQITLKGCDGAVLKIKGEP"
     gene            complement(8327..8566)
                     /locus_tag="ACZPCG_00365"
     CDS             complement(8327..8566)
                     /locus_tag="ACZPCG_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaL family protein"
                     /protein_id="HIR1283559.1"
                     /translation="MSDWMKAVCQTAAATLKKQDPQLLRLRAEIEDTRHAIETARNHF
                     EQEVDPTLIDCCIYEWNAAQLRYQFLLRKFKSREL"
     gene            complement(8695..9048)
                     /locus_tag="ACZPCG_00370"
     CDS             complement(8695..9048)
                     /locus_tag="ACZPCG_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR1283560.1"
                     /translation="MTTAAELQKQFHSCMPIFIALGDKVRLSIIEALTENAMALDSPG
                     VSVDFADHAMNVNEITRRTSLSRPAISHHLKILKAAGLVDVREDGTANYYYLTLREPT
                     RQLMELGFRIEEFIL"
     gene            9418..9990
                     /locus_tag="ACZPCG_00375"
     CDS             9418..9990
                     /locus_tag="ACZPCG_00375"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HIR1283561.1"
                     /translation="MRRFFWKEEYSLYFVCKFLYWLFMAAAAAGDIREYRISNRYLGF
                     ALIAGISGALTWGEGSMAERSLFAAGFVIRLITVVILGFPIYRRGMIGAGDVKMAALF
                     TAWLGVQDGLKALTIGFMLGAVLALLKMLRQGSMVKRFLYLSAYIRHSFQSRMMEIYY
                     DPSRDGREAVIPLGACFFVGAAAAEIWQRL"
     gene            9975..10100
                     /locus_tag="ACZPCG_00380"
     CDS             9975..10100
                     /locus_tag="ACZPCG_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1283562.1"
                     /translation="MAEAVNGEPEAVGGGAWQRRRGIHRPGKAEKGRKSEIQQEE"
     gene            10103..11104
                     /locus_tag="ACZPCG_00385"
     CDS             10103..11104
                     /locus_tag="ACZPCG_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase family protein"
                     /protein_id="HIR1283563.1"
                     /translation="MVKIMAVYDEDALYARRLAEYVNRRETIPFTAMAFSNLERLKEY
                     GDSHEIEILLVGETVKAAADEVKAGLKMVLCGGEFSIGKEEPSIYRFQSGEGILREVM
                     TCYCTAPPEPALALSGMGAQILGVYSPVNRCGKTAFALTLAQVLSRTESVLFISLEEF
                     AGFEALLGEDVKQDFSDVLYLCRQGSFNWMKLRAMVCSVGQVDMIPPAAYGEDLDQME
                     PEELALLLRRIARESGYRRLVVDMGHMGRGSAALLDCCDGVYLPVLEDPVSRAKLQAF
                     DRYLSAAGAVSLKEKICRLRLPKTRPLSSMENCMDQLLWGEMGDFVRALLEGGGGND"
     gene            11097..12299
                     /locus_tag="ACZPCG_00390"
     CDS             11097..12299
                     /locus_tag="ACZPCG_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HIR1283564.1"
                     /translation="MTERNPREGIREELRQKIMEQLQECSRMEDDELYEQIDRAIREK
                     GQELFLPLKERLWLRSSLYDSFRRLDILQELLDDRNVTEIMVNGAGKIFIERKDGTAL
                     WERRFERPEQLEDIIQQIVGRVNRVVNVSSPIADARLEDGSRVHIVLPPVALDGPVVT
                     IRKFPEPITMEKLIGFGAVTEEAAQFLEGLVAARYNIFVSGGTNSGKTTFLNALSSFI
                     PRTERVITIEDSAELQISQVPNLVRLETRDANTEGKGQITISQLIRASLRMNPDRIIV
                     GEVRGAEALDMLQAMNTGHPGSLSTGHSNSARDMLNRLETMVLMGARLPLEAVRSQIA
                     SALDILVHLGRLGDGSRKVLSIVEVRGISMGEIETEELYRYDRETGRLEKTGELKNRE
                     KLGDTGNC"
     gene            12448..13146
                     /locus_tag="ACZPCG_00395"
     CDS             12448..13146
                     /locus_tag="ACZPCG_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002605688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR1283565.1"
                     /translation="MGLSAIGLGLCGLTAYVFYRSFFAFILLMPLGFLYPIYKKKDLK
                     KKRLRQLTLQFKEAIQVLSSFLSAGYSLENGLFMSVRELEALYGKDAMITEEFERLAA
                     GVRMNRPVEQLLAEFGERSGIQDVDHFAQVFGMARRSGGELVEIIRQSAGVIRDKIQV
                     QEEIQTMTAARIFEQKIMNGIPFGMILYIDFTSPGFFDIMYGTWMGRLIMTLCLAFYM
                     GAVMLSGKILDIEI"
     gene            13159..14433
                     /locus_tag="ACZPCG_00400"
     CDS             13159..14433
                     /locus_tag="ACZPCG_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1283566.1"
                     /translation="MKKWSGRREGFWKRETMSRWKKQLACAAAGLFLGILAQYSEGFD
                     PVLKDGYILNRNPLGQEEETYLLEVRGLADSREPERLELILEAEQYTEKEAETVYEQI
                     IGELPGYILGDNPSLEEVRLPLKLISELPEYGVDLSWRSGSPDLLGSDGQIHGEGVPE
                     QGEQAVLFVRLTDGNWPREYEIPVRIKPPLMTPVQRAAKDLEEQIKEMEQDSRYESAF
                     SLPQFQEGNRLSYDIPGGLPPILSLTALGLAGAFLITLKETVDLKKQEERRRQQMLLD
                     YSEVLSRLIIFLGAGMSIRSAWERIAVDYRRSVEHGVHQKRYIYEEMVLTESQLKSGV
                     SESQAFYEFGSRCGLQPYMKLAALLEQSRKNGARSLRERLRLEMADAFEQRKHQARRL
                     GEEAGTRLLIPLFLLLAVVMVMIAVPAWLAFG"
     gene            14475..14672
                     /locus_tag="ACZPCG_00405"
     CDS             14475..14672
                     /locus_tag="ACZPCG_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006778191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp1 family type IVb pilin"
                     /protein_id="HIR1283567.1"
                     /translation="MELQKDRENLKDIRAFAADESGVGVIELVLILVVLIGLVIIFKK
                     QINTLLENIFKQINSKSKEVY"
     gene            14672..16609
                     /locus_tag="ACZPCG_00410"
     CDS             14672..16609
                     /locus_tag="ACZPCG_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5702 domain-containing protein"
                     /protein_id="HIR1283568.1"
                     /translation="MRHRGEVTVFLTMILVSIMTLLLVVVESARETGARLYLRMAADS
                     SMDSVMAQYHRGLWENYRILGLETGEKERLEQEFGDFFAPYAEAENWYPVTLVDTDRK
                     DAVMLTEGQGAYFEQEILDYMKFGLIDMIWDSMDENGAREMLSQFKDAEGINGCSVRY
                     EGHTREAVRVEAALEKINGRLEEQRQNWEQGKRCLDDLDGSGLIRRAESMIKILKKLP
                     SLVTAYEKRADRLNEALKESRQDFESREDLSDGSREALREEFAQYKTYTEKDGQRRME
                     IGALREKSQENILFLENIIREAEDVMDYIDSWEPDDEDDELDEEALWAPVRSHFSRYP
                     MLSLGMEFGVKDKEKEGWLERIGDLASKGILELVLPEGTVVSGRSLKLNGFPSKERTP
                     GNPAGEGKRNLGVQTLLDRLLVSEYGIRYFSAFKPEPPSFYQLEYILYGKEKDRENLE
                     ASVLRLTALRQGMNLAHILRDPVKREEARSLAIVIAGGTGMAPLAAVLSFFIMTVWAL
                     GEALADVRCLLEGGKVPLVKSSSQWKLDLQGLLDLGAKGRLPDGIGKGEKEEDGLDHK
                     GYLRLMLFGGYSEERVYRMMDVIQTELRTEQPDFCMAGCTCMVEMEAEFCGKPVFFSG
                     GPWKDRQKIRMAVSGSYLDDE"
     assembly_gap    16817..16916
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16917..17302
                     /locus_tag="ACZPCG_00415"
     CDS             <16917..17302
                     /locus_tag="ACZPCG_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1283569.1"
                     /translation="VRRLWTGAEGNRKGNLLPGEDEMEEMVYVGRNSTRYHRNRNCHY
                     LFNDLKQVQAGEMDGLRNQEGKRYYPCPSCKAADSKGPFFIMPWGTRYHSTGQCGAIR
                     AYVQTVPLSEVEHLGECSYCGRGSK"
     gene            17302..17805
                     /locus_tag="ACZPCG_00420"
     CDS             17302..17805
                     /locus_tag="ACZPCG_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1283570.1"
                     /translation="MEQWFETAIVTAFWTFTALAAWQDLKWGSIHIGTFIVGGATGIL
                     LQAGRFAAVSGALGSWRAVCTPAYAVLQLLGAACVGIFLLSLAYVTQEAVGLGDGFFF
                     LAAGCYLGFIKTVFLFSASLLLCFPVSSFLMVRRIWKGDKAADRGKERLPFLPFVLLA
                     AVFLLFW"
     gene            17824..18225
                     /locus_tag="ACZPCG_00425"
     CDS             17824..18225
                     /locus_tag="ACZPCG_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1283571.1"
                     /translation="MEKHLKGSFTVEAAGVMAVILFGIGTIITEAGRIHDQVSCAMTV
                     HEAAEKARHEKTVDLEEIEAFFQKNKGLRLHMKDDRLSLREEGKWIRAAGRAENWEKE
                     IRLRRFRPETFLRKITLIEKLGEEDEDQLYP"
     gene            18203..19654
                     /locus_tag="ACZPCG_00430"
     CDS             18203..19654
                     /locus_tag="ACZPCG_00430"
                     /inference="COORDINATES: protein motif:HMM:NF039422.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6382 domain-containing protein"
                     /protein_id="HIR1283572.1"
                     /translation="MKISYIREMKRSYLTIETEDEEQREKGAWEAGMLEQNRIRGLLP
                     MRVRYEEGREIYCYDITSRQPLERILETHAMTGEQVRSLFWQLYRALEEMEKYLLESG
                     GILLTPELIYAEPERFEIGLCAVPGGKGDFAAELSHFLQYLLKHVDHRDKDCVVLAYG
                     LYQESLKENYGIEDLVKIVAEEKTGRPPQEAGQKAERIKGIQKTGEDEVRDTSGALQK
                     AEGRSNKTALPRQTSTDLPQEAAGQTELCEKKRQSEKNIGKQLLKRMAATAGMFSLIS
                     LGIWFLRGIETLLNLSFYLVIGAAVAEVFTILAVIFWERREKKPADTEKQIKNTRDEE
                     DPWRILYEDEEGAEEEIENSSTESSLRINAASIASPPPEDFQTTVLSVHPEAEECHGL
                     TALHPEISSIEIPYYPFVIGKHKELADYVLDYKTVSRFHLRLDRTEDKITVTDLNSTN
                     GTTVAGVLLAANETAELNPGDEVRIADLRFVWK"
     gene            complement(19663..21066)
                     /locus_tag="ACZPCG_00435"
     CDS             complement(19663..21066)
                     /locus_tag="ACZPCG_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 4 glycosyl hydrolase"
                     /protein_id="HIR1283573.1"
                     /translation="MNYSNNKVSDIQIAYIGGGSRGWAWTFMTDLALDDQISGTIRLY
                     DIDRQAAENNAVIGNHLMSREDAKGDWKFEVKDSLQDVLTGCDFAVISILPGTFDEMA
                     SDVHLPERLGIYQSVGDTAGPGGMIRALRTIPMFVEIAEAIRDYAPDCWVINYTNPMS
                     LCVKTLYHVFPEIKAFGCCHEVFGTQKVLKGIYEAETGNTIEDWHDVHVNVVGINHFT
                     WFTEANYKGVDLFPVYKNYISKHFEDGYELEGENWMNSSFKCAHRVKMDLFNRFGLIA
                     AAGDRHLAEFMPGNEYLNDPETVHSWKFGLTTVDWRKNDLQKRLERSHRLANAEEEVT
                     LEPTGEEGILLIKALCGLDRFVSNVNIPNTALQIPNLPANALVETNALFDLDSIHPVA
                     AGALSEDVKALIMPHVENHERILKAALTCDEELVVTAFMNDPIVKGKNPSEQEIRELV
                     HDMLVNTKAYLPEGWKL"
     gene            21262..22224
                     /locus_tag="ACZPCG_00440"
     CDS             21262..22224
                     /locus_tag="ACZPCG_00440"
                     /inference="COORDINATES: protein motif:HMM:NF024242.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR1283574.1"
                     /translation="MGENTEKDTIYLTSEQLGEEPEILFQEEISGIFAEEAVRRHSFS
                     MVSRHFHQSLELYFLLEGERFYFVEQDTYHMKEQMAILVDQNQIHKTCPAGNGKSHHR
                     FLLQLEGEALSCLLKLCGFRGLLEFGEQYRGITCFSDGEWKQVLEQLEKIKKEMSMSQ
                     SAPGWSDTEKRTAEGMVRLYTAQLLLLLIRTRSRAEAEGWKEPGQTNIVHTGMYQKVH
                     EIAIYLQNHSAGKLCLDDLAAHFFISRSYLTRIFRTVTGFTVNEYQNICRIKKAQILL
                     RETSLTMTEIAMETGFGSLPYFERVFRKATAQTPMQYKKSHPSP"
     gene            complement(22209..23711)
                     /locus_tag="ACZPCG_00445"
     CDS             complement(22209..23711)
                     /locus_tag="ACZPCG_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1283575.1"
                     /translation="MLKLIIADDERIIRETISTIINWKQYDIELVGLCKNGLEAYDTI
                     LDETPDIVLTDIRMPGMDGLELIRRISETDLNTQFIILSGYGEFEYAKMAMKYGVRHY
                     LLKPCSEVQILESIQEIAQDCYRKSSLERSYPAMSSIRHNVIFTILNNAICQNRPYDE
                     IMRAYDPYMDFYFSSYHLFYIYFLEQKDLEEFLQQLKPYADSHFPEVTIHGIYVNCTL
                     LLFFKEFAGNYQDLSQFFSSIKFPGRSPSLETEDLSFKNLQTLLETVLDKVKRFSMIY
                     YINDFRILSSCNYSFIMEEAEKQLSEALQTGRTESADSLINLLSEVTDLSFLKQLSNS
                     LLLKITASDNRLSTIDLTEWLLNVNRERNLEQLKLTLAEKLRKLLSAKPQTSSISAMT
                     QQICDYVNEHLSDPNLTLKRIAEQHLFMNTDYVSKKFQKETGTRFSSYLTDVRIERAK
                     QLLVSAESDKIQTIAEQVGCGNSPQYFSQLFKKKTGMTPSSYIARIHGEG"
     gene            complement(23705..25534)
                     /locus_tag="ACZPCG_00450"
     CDS             complement(23705..25534)
                     /locus_tag="ACZPCG_00450"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF018305.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1283576.1"
                     /translation="MSYGSHGFISFAARSIRYRLNNLPLFQKILFIVLNNVFLIMVLS
                     LFGLRLYTGAYNELLYKTTAGNLTHSAYTISDSLKSIENVSSTILAAPEIQISLSAIA
                     ELNDRAAWTNANRVIINSLQTYHSSVKDNGASFLMIQNEHFHNSTYTMWKSKLSEERL
                     QEAIEVAEQKKGAVVWIPADPGSNALLMSREIRKVDNLSLKHLGTLVVYVDLNNIIRK
                     ANAAATQYSDSQYILCSKDQKIYVPEEFSDSLADKIISSSSEKYQTLKLDSHTYFTVH
                     NTIPGYGLEYYSLVSYDEIIHTLHTSAAFILFILFAGVVLIVFISYCLIRSILKHIKI
                     LLKKMDKFSRDEAALLSTKYDSEYAYRKDEIGRLHQGFERMTARIQNLVNTNYVNEIL
                     AKEAQIKALESQINPHFLYNTLESINWRAKAVNNREISLMTESLGSLLRATLSNKKSL
                     VTLSYELNLILSYITIQQIRFEERLDYREQVPDGLKNALIPPLTLQPLVENAIHYALE
                     EMTETCYITILAAETDKTGKNGERCIRISVTNSGSSFDEDLLELLKQHKKQPSRSGIG
                     LLNIDKRIKLLFGEDYGLSLFNQEDCAVASITIPYRTEETVSC"
     gene            25763..27121
                     /locus_tag="ACZPCG_00455"
     CDS             25763..27121
                     /locus_tag="ACZPCG_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002600594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1283577.1"
                     /translation="MKKRNIALVMAGLMAAMSLTGCSGGAQEEASADGAAKTEAAGSE
                     AADGGEKSLTVAWWGNQVRNERTQAALDKYTELNPGVTLDGQFSEWGDYWNKLATASA
                     GNSLPDVVQMDYAYLDQYAKNNLLLDLTPYVEDGTLNLDDANADVLNSGKVDGKLYAV
                     PIGINAPALLYNKAVTDAAGVTIKDNMTLDEFIEVCKTVKEKTGYRTNMAYGVTQEIV
                     QYMIRANGHSMYGEGKLNVESADEFVPIFKVFEDGVKEGWHVDPSIYAEITPGSVEQD
                     PFVYGASEDSRSWCVFCWSNQIGAYENCKPEDMPEIGITTWPSADPAKSDFLKPAQFF
                     SVSANAANPEEAVKIIDYWTNSMDANNILLAERGVPLSEKVAAELSPSLDPAIQESIK
                     FINEVVSPASSQIDPPMPNGSSEVTELLLQLEEKVAYGQMTSEEAAQELFTKGNEIMA
                     SK"
     gene            27224..28114
                     /locus_tag="ACZPCG_00460"
     CDS             27224..28114
                     /locus_tag="ACZPCG_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002600593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1283578.1"
                     /translation="MNPFKKALSKESTAGIIFTLPFTIGFLLFMIVPMGISLYYSFCK
                     YDILSPPVFTGLDNFVKMFQDETFYKAIGVTFYFALVSVPLRLVFALIVAMLLLKNSK
                     MSGFYRAAYYLPSIIGGSVAVAILWKRMFATDGVVNRLLGAVGIETTFAWLGNTKTAI
                     WVLILLAVWQFGSSMLIFLSALKQISKELYEAAEVDGANGIQKFFRITLPLLTPTIFF
                     NLVMQMINGFLAFTQSYIITQGKPMNSTLFYTVYMYQQSFEFYNTGYGAALAWVMLAI
                     IGAITLLLFATKKFWVYEEG"
     gene            28118..28963
                     /locus_tag="ACZPCG_00465"
     CDS             28118..28963
                     /locus_tag="ACZPCG_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1283579.1"
                     /translation="MKEKRAVKAVIYHILVFAVGLVMIYPLIWMVMSSFKPTNTIFQT
                     AGSLIPETFTFENYINGWKGFAKVTFATFFKNSLFISVVATIGTVISSAIVAYGFARF
                     KFKGKKLLFSAMLLSMMLPAQVLMIPQYLWYQKLNWVGSYMPLIVPYFFAIQGFFVYL
                     ISNFISGIPRDLDEAAKIDGCSYVSIFTKIILPLIKPALVTAGIFSFMWRWDDFLSAL
                     LYVNKSAKYPVSLALKLFCDPGSSSDYGAMFAMASLSILPSVLIFIFFQRYLVEGIST
                     SGLKG"
     gene            29066..30112
                     /locus_tag="ACZPCG_00470"
     CDS             29066..30112
                     /locus_tag="ACZPCG_00470"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88/105 protein"
                     /protein_id="HIR1283580.1"
                     /translation="MKLNYDKAQLEAVVDKVAERTMNMDMTWDWPCGVAYYGITEAYE
                     ATGNKKYLDMVKERVDELISLGLPDFTVNTCAMGHVLLTLYKETGDETYKKIIDDKLD
                     YLTNHALRFGDHVLQHTVSANNDFPEQCWADTLFMSAFFMLRAGVMFERPELVEDALN
                     QWYWHIQYLQNERTGLWYHGYDNIAKDHMSGFYWGRANCWAAFTMSQVAHILPQCYLY
                     PKYLEIVGSLNEQLAVLKTLQTENGLWRTILDDPESYEEVSASAGIAAAMVLKGNPLH
                     IKYINKAITGILENISDDGKVMNVSGGTAVMKDREGYRSISKRWIQGWGQGMALAFFS
                     TVLNYEKFTGDGAL"
     gene            30144..32369
                     /gene="gnpA"
                     /locus_tag="ACZPCG_00475"
     CDS             30144..32369
                     /gene="gnpA"
                     /locus_tag="ACZPCG_00475"
                     /EC_number="2.4.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006773802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050500 -
                     1,3-beta-galactosyl-N-acetylhexosamine phosphorylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,3-beta-galactosyl-N-acetylhexosamine
                     phosphorylase"
                     /protein_id="HIR1283581.1"
                     /translation="MEHTKYPHTKGSFTLPGEAGCEALTLELADQWGADVIRDSDGTR
                     LSDEIVNAGYGIYSTICIIRDHNEWARAHMDRLQQTFLMSAPKTAKADTVIIGLLDGY
                     FKEQFLVNGSEDSLEFWQVFDRTDNAEVPREQWTFDEAQGTVTVAGIRAYHDYTVNFL
                     AYRIWEEINMYNHVTNHWDKEHLMPVDPRYPDTQEYLLSWLKNWCGENPDTTVVRFTS
                     LFYNFVWIWGEDERHRNHFTDWGSYDFTVSPRALKEFEKEYGYGLTSEDFINQGRLHT
                     THMAPGKKQLDYMKFTNRFVISFGKQLVDLVHSYGKKAYVFYDDSWVGLEPYFPTFTE
                     FGFDGIIKCIFSGYEVRLCAGVDAPVHEVRLHPYLFPVGLGGAPTFSGNGDPKRDAEK
                     YWIAARRAMLRAKIDRIGLGGYLHLLEDYPDFVEYISRTADEFRAIKALHEEGEPQLL
                     RLHVGVLTAWGKTRSWTLSGHFHETWQNDLIHINEALSGWPVKVDYIDFEDIREQELS
                     DYDVIINAGFAGSAWSGGEQWNDSRVLEKLRSYAEEGGCILGVCQPSMIKGYMTNFRL
                     ADVLGVDQDTGERCCHGRISYKKAEKFFGDDSVFSKKELDSIAGSLKGTEGIYLTDFN
                     VQVLAEDVAYSPIGEEVRSPKLTYHPYGKGCGIYLSHFTYSVENSRFLLELLLKAAGK
                     EEAKAQLGDNIYCEAAYFPCSGKAVAVNNSDRPQHLKITLGGAVKEVDLEPCGSCMIE
                     I"
     gene            32432..33442
                     /locus_tag="ACZPCG_00480"
     CDS             32432..33442
                     /locus_tag="ACZPCG_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIR1283582.1"
                     /translation="MLRKNDQKRRNLKAYDQFASNVRRYVMETRRLGAVPVLVTPMSR
                     IPVRDEKGWYDLLEAHADSIRRVGQEMNVPVIDLHSLTFEAFCSMGPETCQNYFNDTT
                     HVNDYGGALLADMMVKEIRRLRIEPLCSHMNHTVSGGWEPDLSLRPQGQAESSKIEER
                     PNLSMDLPELPYEDCRSLTKKDVEMLKQAMKCGLLDPCVRYLHPLEEMPRAQFVFLFL
                     KAVKPTLRRPWQGEYCDLSRYEYDAEQIQAAWDENLIDPETTPDDRFRPDDPLTLGEL
                     ISFTVRAFRPKQQRRIGSLECIREAEKLGWIKDTCQDMNRVVTRAEAIQMTVNLYCEK
                     FT"
     gene            33653..34081
                     /locus_tag="ACZPCG_00485"
     CDS             33653..34081
                     /locus_tag="ACZPCG_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HIR1283583.1"
                     /translation="MTDDKCIFCKIANGEIPSATVYEDDMFRVILDLGPATRGHALIL
                     PKNHFADVCALDEKTAAKVLAIGAKVGAAMKEGLGCAGFNLVQNNGEAAGQTVMHFHM
                     HVIPRYEGGPNIVSWTPGAVSGDDLTELAEKIKGSFRKQE"
     gene            34078..34626
                     /locus_tag="ACZPCG_00490"
     CDS             34078..34626
                     /locus_tag="ACZPCG_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR1283584.1"
                     /translation="MIKKMDEKARRRFEQMYQEYEGTVRRLAYGYNIPVDYIDDIVQD
                     TFVSYAQYDYSLEQTETGKRILLGRIVKSRCVDYRRRLENRTCEDVDNDDFIMEDFRI
                     QNRKQSIPEYVASKERCIAILDAIENMPDNWQKVANLKLIQGRPTEEVCQILNISEKA
                     CYSRVSRIRKYMETLLGNEMWP"
     gene            complement(34633..35703)
                     /locus_tag="ACZPCG_00495"
     CDS             complement(34633..35703)
                     /locus_tag="ACZPCG_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HIR1283585.1"
                     /translation="MKKIILTGGGTAGHVTPNLALLPSLKEAGYEIRYIGSYQGIERK
                     LIENAGIPYDGISSGKLRRYFDLKNFSDPFRVIKGYAEAKRLLKRYKPDVVFSKGGFV
                     AVPVVLAAKHYKIPTIIHESDMTPGLANKICIPSAARVCCNFPETMKYLPADKAVLTG
                     SPIRAELLKGERTSGLAYAHLKEEKPVLLIIGGSLGSVAVNTAVRRILPRLLPSFQII
                     HICGKGNLDESLIGTEGYVQYEYVDAPLKHLFAAADIVISRAGANSICELLALRKPNL
                     LIPLSAAASRGDQILNANSFAKQGFSKVLEEEVITDDSLCEAVTELYANRSSYISAME
                     TSKLNHAVETIMTLINECSEGK"
     gene            35839..36057
                     /locus_tag="ACZPCG_00500"
     CDS             35839..36057
                     /locus_tag="ACZPCG_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1283586.1"
                     /translation="MEKFNSKKPDQNDNMQREPGEISGFSDEQVLREFEEAMKSTIPL
                     PPPPENEFEQICQRIEKENRMEQIDGFL"
     gene            36125..36520
                     /locus_tag="ACZPCG_00505"
     CDS             36125..36520
                     /locus_tag="ACZPCG_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR1283587.1"
                     /translation="MIGRYGADQLGRFLSIVIVILAAVSFFIRSYWFGSLIRSVLIIL
                     LAILYFRIFSRNITRRSQENNAYIRLHFQVSERLKGWRFHLEERRRYKIFSCPGCKQK
                     IRIPRNHGKVSIRCPKCGHDFIKNTGAKK"
     gene            36727..37671
                     /locus_tag="ACZPCG_00510"
     CDS             36727..37671
                     /locus_tag="ACZPCG_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR1283588.1"
                     /translation="MKKNSKMILAAVGTAVMAAGMLAGCGSSSKETTAAQTTTAAQTT
                     TVADSKAADTTEAKKEEKTEAAADLSGTISMVGSTSMEKFANALSETFMEKYPKVTVT
                     AEFVGSGAGVEAAANGTADIGNASRNLKDEEKAKGIAENIVAIDGIAVVVDPSNTVED
                     LTKDQLAGIYDGTVTNWKDVGGNDAPIVVVGRESGSGTRTAFEELLELEDKCKYANEL
                     DSTGAVMAKVASTPGAVGYVSLDVLDDTVKAVKLEGAEPTEENIKAGTYFLSRPFVMA
                     TKGEISEQNDLVKALFDFIYSEEGAELVKSVGLISVEK"
     gene            37699..38634
                     /gene="pstC"
                     /locus_tag="ACZPCG_00515"
     CDS             37699..38634
                     /gene="pstC"
                     /locus_tag="ACZPCG_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HIR1283589.1"
                     /translation="MTTETCSIRSHHGSKSGVEKAAQVIFTACGFFAVLAVASITLYM
                     IISGTPALFQVGLTDILFGSVWKPDAADPSFGILYVILTSIVGTFLAILIGVPIGVMT
                     AVFLAEIAPKKIASVVRPAVELLAGIPSVIYGLLGILILNPLMYKLELIIFKGTDHQF
                     TGGANLISAVLVLALMILPTVINISESALRAVPLHFRSASLAMGATRIQTIFHVVLPA
                     AKSGIITAIVLGTGRAIGEAMAISLVSGSSVNLPLPFNSVRFLTTAIVAEMGYASGLH
                     RQVLFTIGLVLFVFIMLINISLTGILKKGDKKNDR"
     gene            38624..39526
                     /gene="pstA"
                     /locus_tag="ACZPCG_00520"
     CDS             38624..39526
                     /gene="pstA"
                     /locus_tag="ACZPCG_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HIR1283590.1"
                     /translation="MTGEAAVRLNKYSEVRESIYGKHVRSSDMLLTGLIYGCTAVSLL
                     ILVGILGYTFVRGIPHVTWSFLSTASSAVKGTFGILGNIINTIYIVVITLLIATPIGV
                     GSAVYLNEYAKPGKIVRMIEFTTETLSGIPSIIFGLFGMVFFGNTIGLGYSILTGSLT
                     LTLMILPLITRTTQEALKTVPDSYRHGALGIGATKWYMIRTILLPSAMPGIVTGVILA
                     IGRIVGESAALLFTAGSGYYLPKNILGKIFESGGTLTIQLYLFMQKAKYNEAFGVAVV
                     LLVIVLVINGLAKYLSHRFNVETR"
     gene            39557..40318
                     /gene="pstB"
                     /locus_tag="ACZPCG_00525"
     CDS             39557..40318
                     /gene="pstB"
                     /locus_tag="ACZPCG_00525"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HIR1283591.1"
                     /translation="MENKVKISAQNLNLYYGENHALKNINLDLYENKITAFIGPSGCG
                     KSTFLKTLNRMNDLVPNVKIEGTVLLDDENIYDPQVDTTLLRKKVGMVFQQPNPFPMS
                     IYDNIAYGPRIHGIKNKAQLDEIVERSLRGAALWDEVSDRLKKSALGLSGGQQQRLCI
                     ARALAVEPEVLLMDEPTSALDPISTLKIEDLMDDLKSRYTVAIVTHNMQQAARIADYT
                     AFFLVGEVVEYASTNELFTMPKDKRTEDYITGRFG"
     gene            40352..41014
                     /gene="phoU"
                     /locus_tag="ACZPCG_00530"
     CDS             40352..41014
                     /gene="phoU"
                     /locus_tag="ACZPCG_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008723666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HIR1283592.1"
                     /translation="MTVRITYEHELEELNKDLREMAFMVEKAIEQTFVAFEDQNYTLA
                     EDVIKGDRTVNDMERAIESRCLSLILRQQPVACDLRQISTALKVVTDLERIGDHASDI
                     AELILRIKSEHAYHIVKHLPLMAEEARKMVHDAVEAFISQDLEETEKIIRMDDRVDSL
                     FNQVKTDVISLLKTSPDQADQGIDLLMVAKYLERIGDHAVNVCEWTEFSKTGALKHVR
                     IM"
     gene            complement(41083..41736)
                     /gene="fsa"
                     /locus_tag="ACZPCG_00535"
     CDS             complement(41083..41736)
                     /gene="fsa"
                     /locus_tag="ACZPCG_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HIR1283593.1"
                     /translation="MRFFVDTANVEDIRKANDMGIICGVTTNPSLIAKEGRDFKEVIS
                     EITSIVDGPISGEVKATTTDAEGMIAEGREIAAIHPNMVVKIPMTVEGLKAVKVLSAE
                     GVKTNVTLVFSAAQALLAARAGATYVSPFLGRLDDISMPGIDLIYDITEIFQMHNIET
                     EIIAASVRNPIHVIDCAKAGADIATVPYKVLEQMTKHPLTDQGIAKFQADYKAVFGE"
     gene            complement(41963..42712)
                     /locus_tag="ACZPCG_00540"
     CDS             complement(41963..42712)
                     /locus_tag="ACZPCG_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HIR1283594.1"
                     /translation="MLKEERQALILNKLHTSGKVVVSQLAADLKVSEDTVRRDLLDLD
                     QKGLLKRVFGGAIPLERPVIGFFDREEADVELKQKLAERALGFLEPDQLVAIDGSSTN
                     LQFAKNIPSDMKLTILTNSYSIAHVCSMKEQVKVIVLGGDLLKDSMTTVGETAAAQAA
                     AYHPDLCFMGVYAVHPEYGMTIPYPEEVSIKSQLIRSSRRVIALVDPVKLNTVSRYHV
                     CGIEAFTALITDGSVEGEMALGYRNRGLDFI"
     gene            complement(42718..43860)
                     /locus_tag="ACZPCG_00545"
     CDS             complement(42718..43860)
                     /locus_tag="ACZPCG_00545"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88/105 protein"
                     /protein_id="HIR1283595.1"
                     /translation="MKILDHYIDQLLDKSTPQAPVWNIEKIKNGGASTWNYIDGCMIK
                     AILELYHITRNGKYLEFADAFIDYFVQEDGSITSYDPKEYNLDNVNAGKTLFDLYELT
                     GREKYRKAIDTVYSQLKGQPRTTTGNFWHKMIYPNQIWLDGLYMAQPFYMQYEVTYNN
                     GANCRDSFEQFLHVYDLMKDTRNGLYYHAYDDSRESFWCDPVTGLSDNFWLRALGWYA
                     MALIDTMEVMPGSMAQEKEKLGAIYKELIDAMLPYQDKETGMWYQVVNRGRIKPNYLE
                     TSGSAIFAYAIMKSVRLGYLDESYFTFGERAFTGICETYLSEENGELQLGGICLVAGL
                     GNKEMREGTFDYYMREPIVKNEAKGVAPLILAYIEILLREKAGTQE"
     gene            44316..45674
                     /locus_tag="ACZPCG_00550"
     CDS             44316..45674
                     /locus_tag="ACZPCG_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1283596.1"
                     /translation="MKRNFKKAVSLALASTMALSLAACGGTSASTDTTAAGKAEGGDS
                     AASNEEVTLKFSWWGGDARHEATEKAIAAFMEKYPNIKVEAEYGAWTGWEEKQALAIQ
                     GGNAADVMQINWNWITSYSGNGKNFVNLDEYSDVIDLTQFDQEVLDQCKADGKLMAVP
                     ISLTGRLFYWNKTTFDEIGCELPTDEASLLAAGEAFKAYGDDYYPLALGEYDRAIFLV
                     YYLESKYGKAWVEDGAVQYTKEEIQEGMDFITKLEENHVIPTLATLQGDMADSLDKNA
                     KWINGKYAGILEWDSSVSKFQQAIAESTDKPGQELVIGDFIKFGDHNGGFTKVSMAFA
                     VAANSEHPKEAAMLINFLLNEDEGIEISGTERGIPCSKKAIEILNEKGIGDEMVKEAN
                     SKVTAYSKFPLDPYFEHNDLKANPDGVYYKVFGKLSAGNTTSAEAAEELLKGIDECYS
                     NY"
     gene            45721..46041
                     /locus_tag="ACZPCG_00555"
     CDS             45721..46041
                     /locus_tag="ACZPCG_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007036252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1283597.1"
                     /translation="MKGTVNMTEKEIREAVKNKENYSSIVTYFKTQKTLSEADLALLI
                     DMIEEMSPEIYEHYRALQDMFREGIRKHLEASKEASGEQLAYAIKKGCALGTLLAEKY
                     ADRV"
     gene            46070..46963
                     /locus_tag="ACZPCG_00560"
     CDS             46070..46963
                     /locus_tag="ACZPCG_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1283598.1"
                     /translation="MKNKKGFWALNIGLLYILPWLVGFILFKVYPFLSSLFYSFTNYD
                     LFNGITKYGMMNYEKIFTDPDIIRAFVVTFKYAIFDVPLKLMFALFIAYILNFKLKGV
                     NFFRTAYYIPSILGGSIAIAILWRAVFNTDGLINTVLGAFGIPEINWMAGDMSALMVI
                     VLLRVWQFGSAMVIFLAALKGVSEDLYEAASIDGAGKWTQFFKITVPLITPVIFYNLV
                     TQLCQAFQEFNGPFLVTKGGPNGATTLISMLIYNNAFLRHKMGMASAQAWILFLITMT
                     MTVVAFVSQKKWVYYSDEDGR"
     gene            46970..47821
                     /locus_tag="ACZPCG_00565"
     CDS             46970..47821
                     /locus_tag="ACZPCG_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1283599.1"
                     /translation="MTRETRRKISAVIRYTLLILVGIIMVYPLIWMVGATFKTNSEIF
                     TSAWFIPKNPVFDGYDNAFKSYGGKIDLVKSLINTYKIVIPKVIFTVVSATITAYGFG
                     RFNFKGKNIFFALLMSTLFLPQVVLNAPQYIMFNNWKWVNTYLPLVIPSLFAADTYFV
                     FMLIQFLRGVPKELEEAAKIDGCNTLKTLWYVIVPMLKPSLVSCALFQFMWTNNDFQG
                     PLIYIADMDKYTNSVYLRMSMDGDVGFQWNRILAMSLISIIPSLVVFFCAQDSFIDGI
                     AAGGVKG"
     gene            47899..49449
                     /locus_tag="ACZPCG_00570"
     CDS             47899..49449
                     /locus_tag="ACZPCG_00570"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HIR1283600.1"
                     /translation="MELKVIYKNARCAVIEIEDGGRYHTASSWTIYLNHKEAAKTDKA
                     VTSLYHLTPDTQYEVEAVNEAGERIMSYFATDYEFVTLNVRDFGAKGDGESDDTIFIQ
                     AAIMACPKESRVLITAGTYRISSLFLKDDVSIELDEGAVLSAFTDRSRFPVFKGMIQS
                     YNEKDEYNLGTWEGNPLPMFTAIITGVNVKNVTIYGQGTIDGNANRENWWKDPKIMNG
                     AFRPRMVFLERCEHVVLQGITVQNSPSWNLHPYFSDNLRFLDMKVLNPKDSPNTDGLD
                     PESCKNVEITGVYFSLGDDCIALKSGKIYMGSTYKRPCENVTIRQCCMRDGHGSITIG
                     SEMAGGVKNLTVRDCMFLHTDRGLRIKTRRGRGKDAVVQGILFENITMDHVMTPFVIN
                     CFYFCDPDGHSEYVRSKEAGPVDDRLPEIATLTFRNIEARNCHVAAAYMYGLPEKKIG
                     RVEMEHVRVTYADEPMCGQPAMMEGCSKTTCRQGIYANNIEELILKDVEISGQEGPAV
                     VTENIGNM"
     gene            49465..50457
                     /locus_tag="ACZPCG_00575"
     CDS             49465..50457
                     /locus_tag="ACZPCG_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR1283601.1"
                     /translation="MAVTIKDIAKKAGVSYSTVSRALNGVGGYSETRDRIRHLASEMG
                     YVPNQAAIHLKKSRSYVIGLYFSTISKMTSPYVLHDVLTGVYSITGSKYNVVVKGIDM
                     HEPGTLNPSYFDGIIVLSQKNEDMEFMDEVLSKKIPMTVICRSVDIDAPNVTTDEARA
                     MERAMDYLLDNGHRKLAVIEGRPELDSTRLRHRGWRISMRKHGLDPDQVPMINGNYRY
                     GSGYSAAKQLLSYEPTAILCFNDEMAFGARTAIDEAGLSVPGDVSLIGFDNWDLSGYS
                     DMHLTTVERNMGDIAREGARVLLRRLDDGIVDNRRIYLDNKLIIRDTVRNLKEE"
     gene            50500..51024
                     /locus_tag="ACZPCG_00580"
     CDS             50500..51024
                     /locus_tag="ACZPCG_00580"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008723691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HIR1283602.1"
                     /translation="MKKLEEYVRSIPDFPEPGIIFRDVTSILQDADGLQLAVDSLRAM
                     VKDLDYDVVVGPESRGFIFGVPVAYAEHKSFVPVRKKGKLPCETISAEYELEYGTAEI
                     EIHKDAIRPGQKVVIVDDLIATGGTTEAIIKLIEQLGGQVVKICFVMELAGLKGREKL
                     KDYAVDSAIVYEGK"
     gene            51220..51711
                     /locus_tag="ACZPCG_00585"
     CDS             51220..51711
                     /locus_tag="ACZPCG_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009244171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR1283603.1"
                     /translation="METMHMGRMIHMLSHQLKRRNIQEELMDEGLTGSQRHIMKFILL
                     RTLHTDVYQKDIEEEFRIRKSTVTGILKLLEKNGFIRRESVPQDARLKRIVPTARAEA
                     LRPQILEQLGRTEALLMEGIPEEELHIWRSVMCRMFGNLVKAEEEASKTNKNIKEEEE
                     KDE"
     gene            51704..53440
                     /locus_tag="ACZPCG_00590"
     CDS             51704..53440
                     /locus_tag="ACZPCG_00590"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1283604.1"
                     /translation="MNKLLLKSVREYKKQSILTPIFVLLEVLLEVMIPMEMAKIIDIG
                     IAGGNLPYIIQRGFILVVMAMLALYFGVTAGKTAAIAAAGYAKNLRHDIFYRVQEFSF
                     KNIDHFSTSGLVTRMTTDITNVQMAYMMSLRLLARAPIMVALSWVMTLTINPKIALLF
                     LVVIPVLGGALIFIAKKAHPHFVKVFDEYDILNNSVQENVNASRVVKSFVREDYEIGK
                     FHDISKYVYKLFTKAEGIVAWNSPVMQTVIYIVIILLVAISGRSIVFGTMETGEMTSV
                     IVYAMQIMMSLMMVTFVFVMIMIAEASTDRITSVLTEVPEMQAKEQAVKEVADGSITF
                     EHVDFSYAGEGGPLSLKDVNLSIKPGQTVGIIGGTGSSKSTLVQLIPRLYDVTKGSVK
                     VGGIDVREYDLEVLRDQVSMVLQKNVLFTGSIYENIRWGNERASNEEVERVCRLAQAD
                     GFVKEFPHGYDTMIVQGGNNVSGGQKQRLCIARALLKRPKILILDDSTSAVDTKTDAL
                     IRRAFREEIPDTTKIIIAQRVSSIEDADQIIVLEEGQIVGVGTSSELLRTNEIYREVY
                     ESQMKGGEENEQ"
     gene            53430..55313
                     /locus_tag="ACZPCG_00595"
     CDS             53430..55313
                     /locus_tag="ACZPCG_00595"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1283605.1"
                     /translation="MSSKKLQISKNTAQTAKRLLKYVTETYKLRFFLVFICIFISSGA
                     SISVSLSLKFLLDDFIIPLIGQQTPDFAGLYRALGTLACIFVAGVLAAFTYTRLMVYI
                     GQGVLKRIRDDMFEHMQTLPIRYFDQNTTGSIMSLYTNDTDTLRQMISQSIPQALSSF
                     FTIVVTFISMLVLSPALTVLAVLVIVVMLFVSRKIGGNSGKYFVRQQVAVADITGFVE
                     ERMNGQRVVKVFNHERKSEEEFDKLNEALFECASQANTFANIMGPVIGNIGNMQFVLT
                     AVLGGFLSVLGVGGITLGVMASYLQFTKSFTQPFMQVAQQFNAIIMALAGAERIFALI
                     DEKPEKDEGYVTLVNARKDADGVISECKERTGMWAWKHPHGDGSVTYTELTGDVRFEN
                     VTFGYDDEKVILHDISLFAKPGQKLAFVGSTGAGKTTITNLINRFYDIQDGKIRYDGI
                     NITKIRKDDLRRSLGIVLQDTHLFTGTIRENIRYGKLDATDEEVYAAAKLAHADQFIR
                     MLPQGYDTMLSGDGEELSQGQRQLLSIARAAVADPPVLILDEATSSIDTRTESIVQKG
                     MDNLMKGRTVFVIAHRLSTIRNSNAIIVLDHGRIIERGDHEDLIRLKGTYYQLYTGKL
                     ELS"
     gene            55511..57796
                     /locus_tag="ACZPCG_00600"
     CDS             55511..57796
                     /locus_tag="ACZPCG_00600"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HIR1283606.1"
                     /translation="MGKEGTKEKLDIELEAPADFTSPEVLYQDLIRAIRKYHPSDDLT
                     MIEKAYKIADEAHKEQKRKSGEPYIIHPLCVAIILADLEMDKETIAAGLLHDVVEDTI
                     MTLEQLTKEFGSEVAFLVDGVTKLTQLNWDKDKVEIQAENLRKMFLAMAKDIRVIIIK
                     LADRLHNMRTGKYWKPEKQKEKARETLEIYAPIADRLGISKIKIELDDLSLKFLHPDV
                     YYDLVEKVDLRKDAREAFVQSIVSEVKEHMDEAGIEASVGGRVKHFFSIYKKMVKQNK
                     TLDQIYDLFAVRIMVETVKDCYAALGVIHEMYKPIPGRFKDYIAMPKPNMYQSLHTTL
                     IGSTGQPFEIQIRTYEMHRTAEYGIAAHWKYKESGSGQVAAGKEEEKMSWLRQILEWQ
                     KDTDDSKEFLNMVKGDLDLFSDSVYCFTPSGDVKTLPSGSTPIDFAYCIHSAVGNKMV
                     GARVNGKLVPIEYVIQNGDQIEIVTSQNCKGPSRDWLKVVKSTQAKNKINQWFKTQMK
                     EDNIIRGREAVERYCKAKGINWAELGKPEFMEKVMHRYAFKDWDSVLASIGHGGLKEG
                     QVINKMMEEREKKRKREITDADVLGGIADTDGRSGEATVRKNKSGIVVKGLHDLAVRF
                     SRCCNPVPGDEIVGFVTRGRGVSIHRTDCINVINLPQEERARLIDAEWQVAETAGSTE
                     QYSTEIQIFANNRIGMFADISKVFTEKQIDITSMNVRTSKQGKATIIMTFDIHGTEEL
                     NRITDKIRQIEGVLDIQRTTG"
     gene            57809..58450
                     /locus_tag="ACZPCG_00605"
     CDS             57809..58450
                     /locus_tag="ACZPCG_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR1283607.1"
                     /translation="MSDFRIKTCVLGPVSTNCYLVYNDTTKEAVAVDPGDNGDYILNK
                     CSELGIKLQAVLLTHGHFDHIGAALQIARAFTIPIYASETEDGMLADTSLNLTAHFTG
                     HSLSFHADRLVHDGDELELLGYKWKVIATPGHTAGSVCYYLPEEEVLLSGDTLFQDSY
                     GRTDLPTGSSRALVDSVLRKLFILPGDTMVYPGHGEPTTIGYEAQNNPIACYR"
     gene            58472..60019
                     /gene="hemZ"
                     /locus_tag="ACZPCG_00610"
     CDS             58472..60019
                     /gene="hemZ"
                     /locus_tag="ACZPCG_00610"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003508590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen dehydrogenase HemZ"
                     /protein_id="HIR1283608.1"
                     /translation="MIGLLIQDNAYEQDIRELLMSFYPGETYAHEIKDGVDFYVQTRL
                     IQKEEDGCAASEEDAAGQEWNVGIALWEQIKEGEAAGCSPYFPVPEGLEDFHLASSSR
                     GYSDLSDHSETKNVVKQLFYRMLTERTGHELPWGSLTGIRPTKIALSRLEEGWKEEEI
                     RSFMKKNYLASDEKIDLSIEIAAREKMLLEPLDYDRGYSLYVGIPFCPTTCLYCSFTS
                     YPISKWKGRTGLYLEALFKEMEYVADKMKGRPLDTIYFGGGTPTALPAEDIDAILTKL
                     DQLFDTENALEFTVEAGRPDSITEEKLRVLRDHGITRISINPQTMNQKTLDLIGRRHT
                     VEMVKEKFLMARELGFDNINMDLIMGLPEEDLDDVRHTLEEIEALRPDSLTVHSLAIK
                     RAARLNMFKEEYGGLKINNTPEMIAMSEACARRLGMEPYYLYRQKNMAGNFENVGYSL
                     PGKACIYNILIMEELQTIAACGAGTTTKVVFPAENRRERCENVKEVEQYISRIDEMIG
                     RKERIWG"
     gene            60133..61398
                     /gene="hisS"
                     /locus_tag="ACZPCG_00615"
     CDS             60133..61398
                     /gene="hisS"
                     /locus_tag="ACZPCG_00615"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HIR1283609.1"
                     /translation="MPLTKKPVTGMKDITPAEMQIREYVMNQIRETYKSFGFSQIETP
                     CVEHIENLTSKQGGDNEKLIFKVLKRGDKLNLETAQDENDLTDSGLRYDLTLPLSRYY
                     ANNASSLPSPFKALQMGSVWRADRPQKGRFRQFTQCDIDILGDATNLAEIELILATTT
                     ALGKICPDNGFTVRINDRGILFGMARYCGFSEEAMDSVLITLDKMDKIGFEGVEKELL
                     ENGNAKESVDRYLELLRTVTRDAEGVKALGETLKDVMDPAVCQGLVHIMETVKDVAET
                     EFGLVFDPTLVRGMSYYTGTIFEVSMEGFGGSVAGGGRYDKMIGKFTGMETPACGFSI
                     GFERIVTILLDNGFTVPGAGEKKAYLFEKGIDSAALAAVIREAMEERKNGVQVLVAQM
                     NKNKKFQKEQLGKEGYGEFKEFYRESLKH"
     gene            61682..63475
                     /gene="aspS"
                     /locus_tag="ACZPCG_00620"
     CDS             61682..63475
                     /gene="aspS"
                     /locus_tag="ACZPCG_00620"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HIR1283610.1"
                     /translation="MAESMAGLKRTHRCTEVTAAQIGENVTVMGWVQKSRNKGGIIFV
                     DLRDRSGILQLIFEENDCGAENFAKAEKLRSEFVVAVEGRVEARSGAVNENLATGAIE
                     IRANSLRILAESETPPFPIEEKSKTKEELRLEYRFLDLRRPDIQHNLITRSRVATLTR
                     AFLAEEGFLEIETPTLIKSTPEGARDYLVPSRVHPGSFYALPQSPQLFKQLLMVSGYD
                     RYFQLARCYRDEDLRADRQPEFTQIDMELSFVDVDDVLDVNERLLQKLFKEICGYELQ
                     LPIQRMTWREAMNRFGSDKPDLRFGMELKNVSEVVKDCEFVVFKGALENGGSVRGINA
                     EGQGHMPRKKIDALVEYAKGFGAKGLAYIAINEDGTFKSSFAKFMKEEEMVALIAAMD
                     GKAGDLLLFAADKDKVVFDVLGNLRLELARQLDLLKKDDFKFLWVTEFPLLEYSEEED
                     RYVAMHHPFTMPMEEDLQYLDSDPGRVRAKAYDIVLNGVEMGGGSVRIHQADIQSKMF
                     EVLGFTPERAQEQFGFLLKAFKYGVPPHAGLAYGLDRMVMLMVGADSIRDVIAFPKVK
                     DASCLMTEAPAPVDGKQLEELGIQVAEEEKQ"
     gene            63643..64002
                     /locus_tag="ACZPCG_00625"
     CDS             63643..64002
                     /locus_tag="ACZPCG_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR1283611.1"
                     /translation="MALNDVECCDFYQVHEDVVKSVTDKMPDEDQLYDLAEIFKVFGD
                     STRIKILYVLFESEMCVCDIAQLLNMNQSAISHQLRILKQNRLVKNRREGKAVFYSLA
                     DSHVKTILSQGMDHISE"
     gene            64031..64249
                     /locus_tag="ACZPCG_00630"
     CDS             64031..64249
                     /locus_tag="ACZPCG_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HIR1283612.1"
                     /translation="MKKKFKLTNLDCANCAAKMENAIKKIDGVRDANVSFMTQKMTIE
                     ADDSRFDEIMEEVQSVCRKVEPDCTIQM"
     gene            64288..66213
                     /locus_tag="ACZPCG_00635"
     CDS             64288..66213
                     /locus_tag="ACZPCG_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003506295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR1283613.1"
                     /translation="MTVKQKRMLIRILGTFGIYIALTVTEHLGLLPFGEKSPVWPALY
                     LIPYFLIGWDIIYRALRNIRNGQVFDENFLMALATVGAFAVQQYSEAVAVMLFYQVGE
                     LFQNYAVNRSRQSISDLMDICPEYANIEEDGQLKQVDPDDVSVGDIIVIKAGERIPLD
                     GRVISGDSMIDTSALTGESVPRRVRAGDEMISGCVNGSGLLRVEVTKEFDDSTVARIL
                     ELVENASSKKAPVENFITRFARYYTPVVVIAAVVLAILPPLFFGQTWSEGIRRACTFL
                     VISCPCALVISVPMSFFSGIGAASKKGVLVKGSNYLEALANVHTVVFDKTGTLTKGEF
                     KVTGIYPSGDAAQETIEKRQDTDKADGWSASQLALLELAALAESSSDHPVSRSIREAW
                     GHSFEHTRIADAEEIPGHGIRVKADGRTVLAGNDRLMKKENIAYKPCPGVGTVVYLAE
                     EGNFLGSILISDVIKEGIPEAIRDLKKAGAAKTVMLTGDRSKTGEAVAKEIGLDEVHA
                     ELLPGDKVDMVEKLLAECKGKEKLVFVGDGINDAPVLSRADVGIAMGSMGSDAAIEAA
                     DIVLMDDDPRKIAAVVRISRKTLRIVMQNIVFALGVKLIVLAMGAFGAANMWEAVFAD
                     VGVSVIAILNAMRALKG"
     gene            complement(66372..66941)
                     /locus_tag="ACZPCG_00640"
     CDS             complement(66372..66941)
                     /locus_tag="ACZPCG_00640"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR1283614.1"
                     /translation="MLKDLVKSCRSYRRFYEDISIPQEELTDMVDTVRLTGCTANCQP
                     LKYRIIRTPEECAAIFPCLLWAGALKDWDGPEKGERPSAYIVILCDLTVAKNKQWDEG
                     IAAQTLMLSAAEKGYGGCMIGSCRRSEILSILKLNPENYSVGLVLALGKPKEEVRLVP
                     VGDDGSTTYYRDENQVHYVPKRSLEDVLV"
     gene            complement(66934..67341)
                     /locus_tag="ACZPCG_00645"
     CDS             complement(66934..67341)
                     /locus_tag="ACZPCG_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HIR1283615.1"
                     /translation="MKIQESAENYLETIYMLSRHKPYVRSIDIANELSFSKPSVSVAM
                     KNLRENGYIRMDEQGHITLTETGQSIADVMYERHILLSNWLIYLGVDPQTAAEDACRM
                     EHVISPESFEAIKKHITQGNELPKELKEESIHA"
     gene            complement(67495..68610)
                     /locus_tag="ACZPCG_00650"
     CDS             complement(67495..68610)
                     /locus_tag="ACZPCG_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1283616.1"
                     /translation="MKKKTAVSLGMLSLALAAGLLTACGSGQKTGAEEGSGEDNKLYV
                     YNWGEYIDESVIDEFEEETGIEVVYDLFETNEEMYPIIEAGAVKYDVICPSDYTIQRM
                     IQNNLLAEINFDNVPNIKEIGPEYMDMAKDFDPENKYAVPYCWGTEGILYNKQRFDEL
                     GIPYPTKWADLWNPELEGEILMHNTLRYAFMAALKIKGYSLNTTDPDQLAEARDMLIE
                     QKPLVQAYVIDQVRDKMINGEAAAAMLYSGDLLYIQDTAKEQGSEFDLEYVLPEEGTS
                     YWIDCWVIPANAEHKENAEKWINFLCRPDIAKKNFEYITYATPNKGAFELLDEELQNN
                     KAVFPDLSQLKNSESVQYLGEEVNDMYNEMWKQVKAN"
     gene            complement(68660..69769)
                     /locus_tag="ACZPCG_00655"
     CDS             complement(68660..69769)
                     /locus_tag="ACZPCG_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009219581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1283617.1"
                     /translation="MKRTPILLTAGALAALMTAGLLTGCGGSGKNGSSGDDNKLYVYN
                     WGEYIDESVIDDFEKETGIQVVYDVFETNEEMYPVIEAGGVSYDVVCPSDYMIQKMIE
                     NNLLAEINFDNIPNYKEIGQEYLDISKGFDPENKYSVPYCWGTMGILYNTQMIEELGV
                     PAPTKWADLWDERLEGEILMQNSVRDAFTVALKSLGYSLNTQNPDELQQAKDELIRQK
                     PLVQAYVIDQVRDKMIGGEAAVGAIYSGEMLYIQNAVKEQEADFTLEYVIPEDGTNYW
                     VDSWVIPANAEHKENAEKWIDFLCRPDIAKKNFEYITYATPNKGAYELLDEELKQNKA
                     LFPDLSALTNCEIIQYPGEEADALYNELWKEVKAN"
     gene            complement(69804..70595)
                     /locus_tag="ACZPCG_00660"
     CDS             complement(69804..70595)
                     /locus_tag="ACZPCG_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1283618.1"
                     /translation="MVKSRKMIRKIISDFYMVLILIFLYAPILTMMVLSFNQSKSRAR
                     WGGFTLDWYLQMFDSRSIMSALSTTLIIAFVSALAAAVIGTAAAIAIQSMRLVPRTII
                     MGITNIPMLNADIVTGISLMLSFIAFGVSLGFKTVLIAHITFNIPYVILSVMPKLKQT
                     DKSTYEAALDLGATPLQAFFKVVFPDILPGVLSGFLLSFTMSLDDFIITHFTKGAGIN
                     TLSTLIYSEVRRGIKPSMYALSTVIFLTVLILLLIVNFRSFKKKD"
     gene            complement(70595..71434)
                     /locus_tag="ACZPCG_00665"
     CDS             complement(70595..71434)
                     /locus_tag="ACZPCG_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1283619.1"
                     /translation="MNKEKKYLDLGKSLLSGPYLVWMTGFTIIPLLMILWYGLTGRDG
                     AFTLENILSIAEPDHMKALVLSLGLSLISTLLCLFLAYPLAMILRGRGNKAGSFIVFI
                     FILPMWMNFLLRTLAWQTLLEKNGVINGILSFFHLPGISIINTPAAIVLGMVYNFLPF
                     MVLPIYNVLAKIDDSVINAAQDLGANTFQTFIKIWLPLSLPGVISGITMVFVPSLTTF
                     VISDLLGGSKILLIGNVIEQEFTRGSNWNQGSGLSLVLMIFILISMAMIAKYDKNGEG
                     SAF"
     gene            complement(71427..72503)
                     /gene="potA"
                     /locus_tag="ACZPCG_00670"
     CDS             complement(71427..72503)
                     /gene="potA"
                     /locus_tag="ACZPCG_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:1902047 - polyamine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein"
                     /protein_id="HIR1283620.1"
                     /translation="MSQPLIDLQHISKSFDGELILDDLNLSIRENSFVTLLGPSGCGK
                     TTTLRILGGFTSPDTGKVLFDGQDITKLPPNKRQLNTVFQKYALFPHMSIADNIAFGL
                     KIKNKPKSYIDDKIRYALKLVNLSGYEKRDVTSLSGGQQQRIAIARAIVNEPRVLLLD
                     EPLGALDLKLRQDMQYELIRLKNELGITFIYVTHDQEEALTMSDTIVVMNQGYIQQMG
                     SPEQIYNEPENAFVADFIGESNIVHGIMLQDELVEIFGAKFACVDKGFGTNKPVDVVI
                     RPEDIDLVEPEKGTLVGTVTHLIFKGVHYEMEVTTPDGFEWLVHSTDMFPVGKQVGIH
                     VDPFDIQIMNKPASEDEEALGVDE"
     gene            complement(72569..73102)
                     /locus_tag="ACZPCG_00675"
     CDS             complement(72569..73102)
                     /locus_tag="ACZPCG_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1283621.1"
                     /translation="MDIGNRLKELRVLKGLTQEELADRSELSKGFISQLERNLTSPSI
                     ATLMDILQCLGTTIGEFFTEEPEEQIVFGTQDYFEKVDPEYNNEIKWIIPNAQKNTME
                     PILLTLKAGGSTYPDTPHEGEEFGYILKGSVSIHLGKKTYHAKKGESFYYTADKKHYL
                     SSKNGAMLIWVSSPPSF"
     gene            73331..74389
                     /locus_tag="ACZPCG_00680"
     CDS             73331..74389
                     /locus_tag="ACZPCG_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HIR1283622.1"
                     /translation="MSGRYMAYVGSYSYTGKAKGITVFDVDVEKGTFKERCEMEVNNS
                     SYVIASSDGKTLYSIADEGVVSFRILQNGALARLNSAPIRGMRGCHLCSDLEDKFIFV
                     SGYHDGKTTILSLNKDGSVGGIVDGVYDKGYGSVAERNFRPHVTCTRRMPDNKYVLSV
                     DNGVDQVKIYRFNDKEQKMVQVDAIRCDLESAPRHFRFSRDGRFLYLMYELKNVIDVF
                     TYETGDRAPVIEKIQTISTTSTKNPGNLTAACAMRMSWDEKYVYCTNAGENTVSIYSR
                     DEETGLLSMICCLPISGEYPKDVAVFPDGKHIASINHDSGSISFFKVDYEKGLLVMSG
                     RSIPVNEPNCCAIVKIGD"
     gene            74392..75507
                     /gene="aroC"
                     /locus_tag="ACZPCG_00685"
     CDS             74392..75507
                     /gene="aroC"
                     /locus_tag="ACZPCG_00685"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006770991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HIR1283623.1"
                     /translation="MAGSIYGTLFRVSTWGESHGKGIGVVIDGCPAGLPLDEEDIQRY
                     LDRRKPGQSRFTTARQEGDQVEILSGVFEGKTTGTPIALEVRNTDQRSHDYSNIMDVY
                     RPGHADFTFDQKYGFRDYRGGGRSSGRETIGRVAAGAVAAKLLERLGVRVFAYTSAIG
                     PVSIDRSRMEISKMWENRLYMPDDIAAKEAESYLEDMMARRDSCGGVVECVIEGLPAG
                     VGEPVFDKLDAALAKAMMSIGAVKGVEIGDGFEAAVSTGSQNNDSFCIDSAGHPAKRT
                     NHSGGVLGGMSDSSPVVIRAAFKPTPSIAQPQSTVDRSGKEREIEIHGRHDPIIVPRA
                     VVVAEAMAALTAADLLLMSMTSRLDRMEEFFERMRRK"
     gene            75510..>75904
                     /gene="rpiB"
                     /locus_tag="ACZPCG_00690"
     CDS             75510..>75904
                     /gene="rpiB"
                     /locus_tag="ACZPCG_00690"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HIR1283624.1"
                     /translation="MKIAMGNDHTAIELKNIIKEFVEGKGYEVIDLGTNSTESCDYPV
                     YGEKVGRAVASKEADLGIVICGTGVGISLAANKVKGVRACVCSEPYSAKLSRMHNNSN
                     VLAFGARVVGSEMAKMITEAWLDAEFEGG"
BASE COUNT        20571 a        15852 c        19954 g        19427 t
ORIGIN      
        1 gcttatggac aggatacggt gattgctgta aagcagttcc aggccagaaa cgatcaggtc
       61 gtagacggct acctgggtcc atctacccgt attgccttaa atgatccgtc ggctcgtcca
      121 aatggattga tgctgggcga acagggcgaa acggttacca gagtacagca gcttttaagc
      181 aaatatggat atttaagctc tgccaatgtg accggatatt atggagaggt aacagagcag
      241 gcggttaaaa acttccagag ccgcaacgga ctggcagcag atggtcaggt cggcatccag
      301 accatgacaa agcttaccag cgacaatgtg agacgtccgg cagcaaactc cggcggagga
      361 agctctgcac agaccggagg aggaagtacg ggaggaagcg gcggaaataa gaagccggca
      421 ggaaataacg gcagccaggg cggttcttcc tctgcgccga aggcaccttc cggtacaggc
      481 gtatctgctt tgatatctgt ggcttcatcc aagatcggaa gtccttatgt gtggggagct
      541 aagggttcca atgccttcga ctgctccggt tttgtatact ggtgtctgaa tcaggtaggt
      601 gttcgtcaga gctaccttac ttcttccggc tggcgcaatg tgggacgcta tacgaagatc
      661 accaacttta atgatattca ggccggcgat attgtggtag taaacggcca tgtaggtatt
      721 gcggcaggag gcgggactgt gatcgacgca tcctccagta acggccgcgt agtacacaga
      781 agcttaagcc agtggtggag aaacaacttt atctgtgcat ggagaatctt ctaaaagcta
      841 cagagaatct ctgaaagaaa gagtcaggca aagccataac acatccgcgg cacatgtccc
      901 gcggatgtgt gcgttttgga aaaaaatggg aggaaataca aatgcagaca attttagtat
      961 gcgacgatga caagcagatt gtggaagcca ttgatattta tcttacagga gaaggctaca
     1021 aggtaattaa ggcgtatgac ggctatgagg ccctggaata tctggaaaat aactccgcag
     1081 acctgctgat cctggacgta atgatgcccg gactggatgg tatccggacg actcttaagg
     1141 taagggaaaa cagcagcatc cccattatca tcctgtctgc caaatctgag gatgcagaca
     1201 agatcctggg attgaatatc ggggcagatg actatattac aaagccgttt aatcctctgg
     1261 agctggttgc cagagtcaag tcccagctgc gccgctatac acagcttggc aacttaaacc
     1321 agcaggagca ggatcaggtt tacaagtgcg gcggccttca gatcaatgat gataataaag
     1381 aggttacagt agacggagaa cccattaagc tgactcctat cgagtataat attctcctgc
     1441 ttttggtaaa gaatgcgggc aaggtatttt ccattgatga aatatatgaa cagatatgga
     1501 atgaggaagc gatcggggca gacaatacag tagcggtcca tatccgccat atcagggaaa
     1561 aaattgagat taacccaaga gagccgaagt atcttaaagt agtctggggc gtaggataca
     1621 agattgaaaa acagtaggtg gacctatgga gcagaacaga aagaaaatac gtattcagaa
     1681 ggtggtttta tcccttcttt cagtgatatt cctcgctatg gccctggcgg gcatcagcgc
     1741 catgtattta aacggaggct acggaagagg gatcagctgg gtctatgagg aaacctacga
     1801 ggattctgat accttcagca tgaagctggg aaaagatata gaaaatattt ttacatatat
     1861 cggctataag gatctctttg aaacaaacgg acagctggat atgagcaagc ccattgtccg
     1921 gcttacggat gggccggggg tagatgagga ctggacactg gatcagatcg tccggtatgc
     1981 caggatcaga ggctactatc tgggagaaaa ctttacggtt cagggttccc ctatgtccat
     2041 ggacgatgac gacatggagg ttacagtcca gtttttgaat acaggctttg attttctgag
     2101 catgggtgaa atggaaaccc ggatgacaaa ggaagaactg gctctggata ttctggaatg
     2161 tctgggacag tattattcta tttattacaa tttcatggag caccagacca atgtgcgctt
     2221 ccgggtagtt tataagagcg atgaaggagt ggaaacggtg tataccaatg taccggaact
     2281 ttcgctggat gagctgagag gatttggaaa atacctgttt attcccggaa ataccattcg
     2341 tatggatacg aatctggcgg tcattcccca gaatgcggct gctcttttgg aaacatggaa
     2401 tccgaataat aatgaccaga attatctggt ggtttcggtt gataccacat atccttatgt
     2461 tgacagctat tccgaagagt ctgcccagta ccgcagtgtg agaaatgcgt ttattatggg
     2521 aatgggcgga gtaattgtcg gattcatcgg tctgtttgcg gcgttagtat ggctggtgtt
     2581 cctgtcggga catacagaca atctggacag cgagatacgc ctttatccgg ttgaccggat
     2641 tcctacggag gtatgcattc tcctgggggc tgtagcagca gcggtgctcc ttttgtgggg
     2701 gaaacggctt ggaatgatgg taatcggtct ttttttcagt gaggaacatc tgcctttctg
     2761 gaataagatg cttcgatatc tgatcattta cagctgtgca gttccctgcg gtttcagtct
     2821 tctgcgccga tacaaggccg gaacgctttg gacggggagt cttggaaaaa gagggactgc
     2881 ggcatttaaa gcttatcggg aaaagaccag ctatgcagta ggcggagcgc tgtgttatct
     2941 tactttttta ggctgcaacg gctttatgat gtggctgatc ctctttttat ttacaaccag
     3001 aggagaacgg atcagctacc ggatcatgta ttatgttgtg cttgtgctgt ttgtatggct
     3061 cgacggctgg gtatttcacc ggctgtttag ggaagcctgc cagagagatc tgctggatca
     3121 ggccatcagt accatttcta ccggagatac gggctaccgt ctggacactt ccatgctcac
     3181 aggcaaggaa aagaaaatgg gagagcatat caataatatt accagcggtc tggacactgc
     3241 tcttcaggaa aaggtaaaaa gcgagcggtt aaaggcagat ctgatcacca atgtgtccca
     3301 tgatatcaag acgcctctta cttctattat taactatgta gatctcttaa agcggcagaa
     3361 aatccaggat cccaaaatcg ccgcttatct ggaggtgctg gatcagaagt ctcagagatt
     3421 aaagacgctg acagaagatc tggtagaagc ctcaaaggcc agttcaggca atctgaagct
     3481 ggaggtttcg gatattaatc tggtagagct ggtccagcag acaaacggtg agtttgaaga
     3541 gcgttttgct ctgcgccgtc tggagctgat ccagacgctg ccggaggaga tcattatgat
     3601 ccgggcagat ggaaggcgcc tgtggagagt tctggaaaat ctgtatacga acgcctttaa
     3661 atatgcccag gagggcagcc gcgtctatgt agacgtaaga gaagagggag ataaggccgt
     3721 atttacgatc aagaatattt ctgaaaagcc tttgaatatc agtccggatg agcttacaga
     3781 gcgatttgta cggggcgatg tggcaagaac cacagaagga agcggattgg gactttccat
     3841 tgcaagaagc ttaacacagc ttcagaaggg agagtttatc atcagtattg acggcgatct
     3901 gtttaaagca caggtgactt ttcctgtgga acggcgggag aggaaggcgg aacgaatagc
     3961 ggaggaaata ccgtctgatt ccagcgaatg ggaagataca taaagaaaaa ggctctccga
     4021 aatcagcttc ggagggcctt tttgaaccct aaataatgtg atatgtaaga tcggtctact
     4081 gtttactgat actcaaatac attgatccag ctctctaaga gctccttttc ctcaggacgc
     4141 ttataggcca gctgagctgc ggctactaca gggagccagc cctgcacata gcgtttttct
     4201 gtaccggttt tttcgcagaa cagatccatg tacagctccg ctcttttgcg gtcctttaag
     4261 cttaacagaa ggtaggttct cgccacatcg gcgctggcat ttccctggga agcatgtacc
     4321 cagtcaatga cataccattt gccatcatca cctacgatga tgttggttgg attgaaatcg
     4381 ccgtggcaga gcttggtatg cttgggcatt ccgtccagtc tggtcatcag ctcatagcgt
     4441 gtgctgtcgt ccagctgctc cacggatttg agggcgcgga tggttttttc cttcagtttg
     4501 tttaacagag ggcaggcctg cttatggatc agaaggtgga gatccaccat gccttccatg
     4561 tattcttcca gcttgtccgg attttcatcc attaactgct gtaaggtctt tccctctacg
     4621 tattccttca ggatggacca ttttccatca acaacagaaa cccccaggat cttaggaacg
     4681 ttgagcgcct cgatatcctc cacccgcgcg ttgcacaggg cttcattcag aacctcggct
     4741 ttcgggaatc catcacagaa ttccttgatg gcagtgctgc cgtcacgata aacggttttc
     4801 ttttcacttg tgaaaactaa ttctcttgcc ataatcatac tctccttcca cacattatat
     4861 acggtactgc gccggtttcc cgccgcactg tacttattct actacaaggg cggtaaaaag
     4921 tacatactaa atttgataaa tttttaacgt acttttggga aatacactgg taacaattcc
     4981 cttttactta aaatcggaag atggccgtac cataagcggg gagaggatag gccacagaat
     5041 agggctttcc atcgcattct cccttgacgg atgtgtagga aggacactga gaagccttgt
     5101 aggcaccgtg ttcattgtcc agtagcagcg tatatttaca ttttttagga actcctaccc
     5161 ggtagtcggg acgggctacg ggtgtgaaat ttataacaaa caacaggttc tttttgccgg
     5221 tttcatctct gcggataaag ctgaaaatac ttcggtcccc gtcgttggca tttacccatt
     5281 caaaaccgtt ccagtcattg tccagacggt acagacaggg atatttttta taaatgtgca
     5341 gcagatcacg gacgtattcc tgaagctttc tgtggctgtc ttcatctgcc agatgccagt
     5401 ccagagccac cttttcatcc cattcatgcc actggccgaa atcctgtccc ataaagagca
     5461 gctttttgcc cggatggccc agcataaagg tatatccggc tttcaggttg gaaaacttgt
     5521 cttctcccag accgggcatt ttgttgatca tggagcattt taaatgaact acctcgtcat
     5581 gggacaacac caggatataa ttttcgctgt taaaataagt caggctgaaa gtcatccggt
     5641 tgtggttata tttgcggaaa tagggatcca gcttcatgta ctccagaaaa tcgtgcatca
     5701 agcccatgtt ccacttaaag gtaaagccca gtccgtcctg ctccggatgc tttgtcacat
     5761 tgggccaggc ggtagattct tccgcgatca tcagtacgcc gagattgcgt ccctgaacga
     5821 cagtatttaa gtgcttgaaa aactcaatgg cctccagatt ctggtttccg ccgtagatat
     5881 tgggtaccca ctgtccgtcc tggcggccat agtccagata gagcatggaa gcaactgcat
     5941 ccactctcaa accatccaca tggtactgct ctacccagta aagggcattg ctgatgagga
     6001 agtccttgac ctcgtttttt ccgtaatcaa aaacctttgt gccccagtcc ggatgttcgc
     6061 ccttgcgcgg atcttcatat tcatagaggg gatggccgtc aaaatcagcc agtccgtggg
     6121 cgtcctttgg aaaatgggca ggtacccagt ccaaaatgac gccgataccg tttttatgga
     6181 gataatttac aaaatacatg aaatcctttg gcgagccata tcgggaggta ggcgcaaaat
     6241 atccggttac ctgatagccc caggagccgt catagggatg ttccgcaatt cccataagct
     6301 ctacatgggt gtatcccatt tccttcacat aggcagccag ctcatgggcg gcctcgatat
     6361 atgtatagca gccatctttt tccgggcggt tctttttacg ccaggagccc agatgaactt
     6421 catagatgct catgggctgc ttttcagggt ctttctgagc ccgcttggtg atccagtctg
     6481 tatctgccca ggaaaagccg gagaggtcgg ctgttacaga agcggtgccg ggtctgtatt
     6541 ctgcgctgaa agcaaaggga tcggccttat aaaggatttt tcccgtatcg gtagtaatgg
     6601 caaatttgta gagttcacct gttttcatgc ctgggttaaa gtattcccag attcccatat
     6661 cagagatttt tttcatggga ttggcatcag ggttccagcc attgaagtcg cctaccagat
     6721 gaacctgctc tgcatgcgga gcccaaacgg caaaataaat ccctgatttt cctttaaaag
     6781 tcatgggatg agcccccagt ttctggtaaa tatcataatg agtgccggct ccgaagagat
     6841 agcagtccag ctccgagatc atacctgttc cggcaggcgc tgaagctctg gaagaagttc
     6901 ctgttttttt tgcggcagcg gttggttttg ccggtgtgct ttttcttgca gccatattac
     6961 ggttccccct tgatcttaag aactgctccg tcacagccct ttaatgtaat ctgcagtttt
     7021 ccgtctcgaa tggggaggat ctcgccatcc tcaagaagat ttacagcctg agaggcagcc
     7081 atgggaacag gaatatttaa tacagcttcg ttatcgtcat tgtttaaagc ggtaatgatc
     7141 aggctgtgtt caccgtgtct ggcaaaggcg tattgacggt tggttaaaag cagttcctga
     7201 tagcgtccca tatgaagctc ttcattctcc tgatgaatga tactcagctt tgaaatacgg
     7261 tctgtaaggg tacagcggag ggaatcaaac gcatctggtg aaatggcagg ccgcagggcg
     7321 tcgtcgcagg agggcgtccg tttcccttcg attccccatt cgcctccgta gtagatggat
     7381 gggatgcccg gaagggtcat caggcaggtg tatacgggaa gcagatgctc ctttgtacga
     7441 agcttactgg caattcgatc ctcatcatgg ttgtctacaa aggtgtacag actgcgtccg
     7501 atagcctcca gacggcgtac attgtgggcg atttcaaaga aattgtggtc gttaaagccg
     7561 gagtaaatgc tcttgtggag ttcgtagttg gttacagagt ggagcatatg gtcattgacc
     7621 cagcggccat agtctccgtg gatgacttct cccatgagcc agaaatcctc tttcatggtt
     7681 ttcgtttccc ggcgcatttc ttccataaaa tggaaatcaa gtacgtttgc gcagtccaga
     7741 cggatgccgt caatgtcaaa ggtgtcgacc cagaaccgga tcacgtcaaa gagataccgg
     7801 cagacctggg gattggcatg gttgagacag ggaagttcaa aatgtccctg ccaggcctca
     7861 tagccgaaag ggtctcccag agggcttgac cagtcaaagt ttacgccctt gtaccagtct
     7921 ttataggggg aatcccattt cttttcctga atgtctttga aggcaaaaaa ttcccgtccc
     7981 gtgtggttga ataccccgtc tacgacgact ctgatcccgt tttcatggca gagggaaatg
     8041 aaatgacgga agtcttcgtt ggagcccaga cggcggtcgg tctgtttgta atttctggta
     8101 tcatagccat ggctggtgga ttcaaaaagg gggcctatgt aaatggcatt gcagcccagc
     8161 gcttttatat gtggaatcca taattccagc tcatcaaacc ggtctgtctg ttctgttccg
     8221 gtattctgct ttggggcacc ggtcatacca aggggataca tatgataaaa aactgctttt
     8281 tcataccaag tgctcatagt tgttgtcctc gcaattctgc cgggagtcaa agctcccggc
     8341 ttttaaattt tcttaaaagg aactggtaac gcagctgcgc tgcattccat tcataaatac
     8401 agcagtcgat cagtgtagga tctacttcct gctcaaaatg gtttctggcc gtttcaatgg
     8461 catgtctggt atcttcaatc tcggccctca ggcgaagaag ctgcggatcc tgttttttaa
     8521 gagtggctgc tgcggtctgg caaacggcct tcatccaatc actcatagaa tattatctcc
     8581 ctgcgcgtct gcccaaaacg gcagagcgga ttttgacgga tttaccggta acacagtcag
     8641 tatatccgaa aaataccatt atatgcagga ttggggaata gaaccgaagt ctttttaaag
     8701 tataaattct tcaatccgga aacccagttc catcagctgc ctggtaggct cccggagggt
     8761 gagatagtag taattggcag tgccgtcctc gcggacatct actagtccgg cggctttcaa
     8821 tatttttaaa tgatgggaga tggccggacg cgacaggctg gtgcgccggg tgatctcgtt
     8881 tacattcata gcgtgatctg caaaatccac cgatacgcct ggtgaatcca gtgccatggc
     8941 attttccgtt agggcttcta tgatggaaag ccggacttta tcaccaaggg caatgaatat
     9001 aggcatacag gaatgaaact gcttctgcag ttctgccgca gtggtcatcc tttgatctcc
     9061 tcctttggtt taaaatttta aactaatgct attttaccat tgtgatgaat gcgggtcaag
     9121 ataaaatggg taaaatgcat aaaaaataaa atggaaaaag tgataaatta ttcattagaa
     9181 cgacacaatt tgataaaatg aacagatgaa gggaaaaata tggtgtaaaa tgaaaatagc
     9241 ccttgacttt tgtcggaggg tggggcataa tgtagtaaca ttcatttcaa aattctattt
     9301 ttaattctaa ttttgtaatt cttatgagag tccatcccct gtcggggaga gaatttcaga
     9361 tttttaagca gatcacagca gaggacagac gcgcaaacag aggggtttca gggcagtatg
     9421 cgtcgttttt tctggaagga ggaatatagt ctgtattttg tatgtaaatt tttatattgg
     9481 ctttttatgg cagcagctgc ggcaggagat atcagggagt acaggatttc caaccgatat
     9541 ctgggatttg cgttgatcgc cggaatcagc ggagcgctga cttggggaga gggaagtatg
     9601 gcagaacgca gtttgtttgc ggccggtttt gtgatccgtc tgattacagt ggtgatattg
     9661 ggatttccca tataccgaag ggggatgatc ggagctgggg atgtaaaaat ggcagccttg
     9721 tttactgcat ggctgggggt tcaggatggt ctaaaggcct taacgatcgg atttatgtta
     9781 ggagcagttc tggctcttct taaaatgctg cggcagggca gcatggtcaa acgatttctt
     9841 tatttatcag cctatatcag gcacagtttt caaagcagaa tgatggaaat atattatgat
     9901 ccctcaaggg acggaagaga ggcggtcatc ccgctgggag cctgcttttt tgtgggagca
     9961 gccgcggcag agatatggca gaggctgtga acggggagcc tgaggcagtg ggaggaggcg
    10021 catggcagag gaggcgtggc atccacaggc cgggtaaggc agagaaggga agaaaatcag
    10081 aaatacagca ggaggagtaa agatggtaaa aataatggcg gtgtatgatg aggatgcgct
    10141 ttatgccaga aggctggcag agtatgtaaa ccggagggag accattcctt ttacagcgat
    10201 ggccttttcg aatctggaac ggttaaaaga gtacggagac agccatgaaa ttgaaattct
    10261 tttagtagga gagactgtaa aagcggccgc agatgaagtg aaagcaggtt taaaaatggt
    10321 gctctgcggc ggagagtttt ccatagggaa ggaggaaccc tctatttatc ggtttcagtc
    10381 gggagaaggg attttgcggg aggtcatgac ctgctactgt acagctcctc cggagcctgc
    10441 actggctctt tcgggaatgg gtgcgcagat tttgggtgtg tactctccgg tgaaccgctg
    10501 cggaaaaaca gcctttgcat tgaccttggc ccaggtattg tccagaacgg aatcggtact
    10561 ttttatcagc ctggaggaat ttgcaggatt tgaggctctt ttgggagaag atgtgaagca
    10621 ggatttttcg gatgtgcttt atctctgccg tcagggaagc tttaactgga tgaagctccg
    10681 ggccatggtg tgctctgtgg gacaggttga catgatcccg ccggcagctt atggggaaga
    10741 tctggatcag atggagcccg aggaattagc ccttctttta aggcgtattg ccagggaaag
    10801 cggatacagg cggctggtag tggatatggg ccacatggga aggggatcgg cggcgctgct
    10861 ggactgctgt gacggggtgt atctgccggt tttagaggat ccggtatcca gggccaagct
    10921 gcaggcattt gacaggtatc tgtcagctgc aggcgcggtt tcccttaaag aaaaaatctg
    10981 ccgtctccgt cttccaaaga cacggccttt gagcagcatg gaaaactgca tggatcagct
    11041 tctctgggga gagatgggag attttgtccg ggctctttta gaggggggag ggggaaatga
    11101 ctgaaagaaa tccaagggag ggaatccgcg aagagctgcg ccagaagatt atggaacagc
    11161 ttcaggaatg cagccgcatg gaggatgatg agctttacga acagattgac cgtgcgatcc
    11221 gggaaaaagg gcaggaacta tttctgcctc ttaaagagcg gctgtggctc aggagcagcc
    11281 tttatgacag ctttcgccgt ctggacatct tacaggagct gttagatgac agaaatgtaa
    11341 cagagatcat ggtaaatgga gccgggaaga tttttatcga aagaaaagac ggaacagctc
    11401 tgtgggaacg gagatttgaa cggccggagc agctggagga tattatccag cagatcgtag
    11461 ggcgtgtaaa ccgggtggta aatgtgtctt cgcccattgc agatgcccgt ctggaggatg
    11521 gctcccgcgt tcatatcgtg ctgcctccgg ttgcgctgga cggcccggtg gtgactatac
    11581 gaaagtttcc ggaaccgatc acgatggaaa agctgattgg ctttggggct gttacagagg
    11641 aagcggcaca gtttctggaa gggctggtag ctgcccggta caatattttt gtcagcgggg
    11701 gaaccaattc aggaaagacg acatttttaa atgccctgtc atcctttatt cccagaacgg
    11761 agcgggtaat taccattgaa gattcggcag agctgcagat ttctcaggtt cccaatctgg
    11821 tgcgtttaga gacaagggac gccaatacag agggaaaggg acagattacc atcagccagt
    11881 taatccgggc ttcgctgagg atgaatccgg atcggatcat cgtgggagag gtaagaggcg
    11941 ccgaggctct ggatatgctg caggctatga acacaggcca tccgggttct ctctcaacgg
    12001 gccacagcaa ttcagccaga gatatgctga accgtctgga aactatggtt ttaatgggag
    12061 ccagactgcc gctggaggcg gtccgaagcc agatcgcttc ggctttggat attctggttc
    12121 atctgggacg gctgggagac ggaagcagaa aggtactttc cattgtggag gtaagaggta
    12181 tatctatggg agaaatagaa acagaggagc tgtacagata tgacagagaa acagggcggc
    12241 ttgaaaagac gggtgaactt aaaaataggg aaaagcttgg agatacagga aattgttagg
    12301 ataaaaggga aaaaatggga gaaaactgcc gtgaaaaccg ggagaaaagg gcagccaggc
    12361 aggaggattt ccctgcgggg cagcccgaag ccccgggctc cggatggaaa ggattaccag
    12421 gtttaccgtt tgtctgctaa agaatatatg gggctttcgg caatcggact ggggctttgc
    12481 ggtcttactg cctatgtgtt ttaccggagt ttttttgcct ttattctcct tatgcctctt
    12541 ggttttttgt atcctattta taaaaagaag gatctgaaaa agaaaaggct gcggcagctg
    12601 acgctgcagt ttaaagaggc gatccaggtt ttatcttctt ttttaagcgc aggatattcg
    12661 ctggaaaatg gtctttttat gagcgtgcgg gaattggaag cgctgtacgg aaaagacgct
    12721 atgatcacag aagaatttga acgtctggca gcaggggttc ggatgaaccg gccggtggag
    12781 cagcttcttg ccgaatttgg agaacggagc gggattcagg atgtggatca ctttgctcag
    12841 gtatttggaa tggcccgcag aagtggtggg gagctggtag agatcatccg gcagagcgca
    12901 ggggtaatcc gggataagat ccaggttcag gaagaaatac agaccatgac agcggcacgg
    12961 atctttgaac agaagatcat gaatggcatt ccttttggaa tgattctcta tattgatttc
    13021 acctctccgg gattttttga cattatgtat ggaacctgga tgggaagact gattatgacc
    13081 ttatgtctgg ctttttatat gggagcagtc atgctgtctg gaaagattct ggatattgag
    13141 atataagggg gcaaagaatt gaagaaatgg agtgggagaa gggaaggctt ctggaaaagg
    13201 gaaacaatgt cacgctggaa aaagcagctg gcatgtgcgg ccgcaggact gtttttaggt
    13261 attttagccc agtattcaga gggatttgat ccggttttaa aggatggata cattttaaac
    13321 agaaatcctt tgggacagga agaagagaca tatctgctgg aagtaagggg gctggcagac
    13381 agcagggagc cggagcggct ggaactgatc cttgaggcag agcagtatac agagaaggag
    13441 gctgagacag tctatgagca gattataggg gaacttccgg ggtatatttt aggggataat
    13501 ccttcactgg aagaggtgcg gctgccttta aagcttattt cagagctccc ggaatatggg
    13561 gtagatttaa gctggagatc cggctctcct gatcttttag gatcagacgg acagatacat
    13621 ggggagggag tgccggagca aggggagcag gcagtgcttt ttgtgcggct cacagacggg
    13681 aactggccca gagaatatga aattcctgta aggataaaac cgcctcttat gacgccggtt
    13741 cagagggctg caaaagatct ggaagagcag ataaaagaga tggagcagga cagtcggtat
    13801 gaaagtgcat tttccctgcc tcagtttcag gaggggaatc ggctgagcta tgatattccg
    13861 ggagggcttc cgcccatcct ttctcttacg gcacttggtc tggcgggagc gtttttgata
    13921 acgcttaagg aaacggtgga tctgaaaaaa caggaggaac gccgcagaca gcagatgctc
    13981 cttgactatt cagaggtttt atcgcgtctt atcatttttc tgggggcagg aatgagtatc
    14041 cgaagtgcct gggaacggat tgctgttgat taccgccggt cggtggaaca tggggtgcac
    14101 cagaagcggt atatatatga agaaatggtt ctgacggaga gccagttaaa aagcggtgtt
    14161 tctgaaagtc aggcatttta tgaatttgga agccgctgtg gcctgcagcc gtatatgaag
    14221 ctggctgccc ttttggagca gagccggaaa aacggggcca gaagcttaag ggaacggctg
    14281 cgcctggaaa tggcagatgc ctttgaacag cgcaaacatc aggccagacg gctgggggag
    14341 gaagccggaa cgagactttt aattcctctg tttcttttgc tggcagtagt aatggtaatg
    14401 attgccgttc ctgcctggct ggcctttgga taaagaacag attgatcaaa taaacgagaa
    14461 ggaggaaaga gcgtatggaa ttacagaagg acagagaaaa tttaaaggac attagggcgt
    14521 ttgcggcaga tgaatccggc gtgggggtga ttgagctggt gctgatactg gtagtcctta
    14581 taggccttgt aatcattttt aagaagcaga tcaatacatt gctggaaaac attttcaaac
    14641 agatcaacag caaatccaaa gaggtttact gatgaggcac aggggggaag tgacggtctt
    14701 tctcacgatg atcctggtca gtatcatgac gcttcttttg gtagttgtgg aatcggccag
    14761 agagacggga gccagattgt atctgcggat ggcggcagat tcttctatgg attcggtgat
    14821 ggcgcagtac cacagggggc tgtgggagaa ttaccggatc ctgggacttg aaacagggga
    14881 aaaggaaaga ctggaacagg aattcggcga tttttttgct ccttatgcag aggcggaaaa
    14941 ctggtatccg gtaactcttg tggacacaga caggaaggat gcagtaatgc ttacagaggg
    15001 acagggagcc tactttgagc aggaaatcct ggactatatg aagtttgggc ttatagacat
    15061 gatctgggac agcatggatg aaaatggagc cagagaaatg ctcagccagt ttaaggatgc
    15121 agaggggata aatggctgtt ctgtccgcta tgaaggccat accagagaag cggtccgtgt
    15181 ggaggcagcg ctggaaaaga taaacgggag gcttgaagaa caaagacaga actgggaaca
    15241 gggaaagcgc tgtctggacg atctggatgg aagcggattg atccgcaggg cagagagcat
    15301 gataaaaata ttaaaaaagc ttccttctct ggtaacggcc tatgaaaaga gagcggaccg
    15361 tttaaacgaa gctttgaagg aaagccggca ggattttgag tcaagagagg atctgagcga
    15421 tggatcaaga gaggctttaa gggaggaatt cgcccagtat aaaacatata ccgaaaagga
    15481 tgggcagagg cggatggaga ttggggcttt acgggaaaaa agtcaggaaa atatcctttt
    15541 tctggaaaat attatccggg aagcggagga tgtcatggat tatattgaca gctgggagcc
    15601 ggatgatgaa gatgacgaac tggatgaaga agctctgtgg gccccggtgc gcagtcattt
    15661 ttcacgatat cccatgctga gcctgggaat ggaatttggc gtaaaggata aggaaaagga
    15721 gggatggctg gaacggatcg gcgatctggc gtccaaaggg attttggaac tggtacttcc
    15781 ggaagggacg gtcgtatcag ggcgcagttt aaagctgaat gggtttcctt caaaggagag
    15841 aacaccaggt aatccagccg gagaagggaa gcgtaacctt ggcgtacaaa ctcttttgga
    15901 ccgccttctc gtgtctgaat acgggatccg ttatttttct gcctttaagc cagagccgcc
    15961 atccttttac cagctggagt atattctgta tgggaaggag aaagacaggg agaatctgga
    16021 ggcttctgtc cttcgtctga cagcattgcg ccaggggatg aatctggctc atattctgag
    16081 ggatcctgtt aaaagggagg aagcccgctc ccttgccata gtgattgcag gaggtacagg
    16141 gatggctccg cttgcagcgg tattgtcatt tttcattatg acggtctggg cgctggggga
    16201 ggcgctggcg gatgtgcgct gtcttttgga aggcggaaag gtacctcttg taaaaagcag
    16261 ttctcagtgg aagctggatt tacagggcct tcttgatctg ggggccaaag gccgtctgcc
    16321 ggatgggata gggaaagggg agaaagagga agatggtctg gatcacaaag gctatcttcg
    16381 gctgatgctt tttggcggat acagtgaaga acgggtttac cgtatgatgg atgtgataca
    16441 gacagaactc agaacggaac aaccggattt ctgcatggca ggttgtacct gcatggtgga
    16501 aatggaagcg gagttttgtg gaaaacctgt gtttttttcc ggcggtccgt ggaaagacag
    16561 acaaaaaata cggatggctg tttctggcag ctatctggat gatgaataaa acagataatc
    16621 tttaggaaag gaggctcatg aggatgctct tttttcggaa tccaacacaa cttcatataa
    16681 cacgctactc cttacgatca atgatccatc aggaacatac ctgtcagaac catcattcaa
    16741 tgaaaatcgc acctctccaa gtacgaacag acacagatat gcagaactat ttaaaaagga
    16801 tccgagtttc cgaaaannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngtgt
    16921 cagacgtctg tggaccggag cagagggaaa ccgaaaggga aatttgctgc cgggagagga
    16981 cgaaatggag gaaatggttt atgttggccg taattcgaca cggtatcata gaaacaggaa
    17041 ctgtcattat ctgtttaatg atttaaaaca ggtgcaggca ggtgagatgg atggactgcg
    17101 taatcaggag gggaaacggt attatccatg tccatcctgc aaagcggctg attcaaaagg
    17161 gccttttttt atcatgccat ggggaacaag atatcacagt acgggacagt gcggggcgat
    17221 tcgtgcgtat gtgcagaccg tgcccttaag cgaagtggag catctggggg agtgcagtta
    17281 ttgcggaagg ggaagcaaat aatggaacag tggtttgaaa cagcaatcgt aacagctttc
    17341 tggacattca cagctctggc ggcctggcag gatttaaaat ggggttccat tcatatcgga
    17401 acgttcattg ttggaggagc cacggggatt ctcttgcagg caggaagatt tgcggctgta
    17461 tcgggtgctt tgggcagctg gagggcagtt tgtactccgg cctatgctgt tttacagctg
    17521 ctgggagcgg cctgtgtagg gatctttctt ctttccctgg cctatgttac ccaggaggcc
    17581 gtgggtcttg gggatggttt tttctttctg gctgccggat gttatctggg gtttataaag
    17641 acagtatttc tttttagtgc cagtcttctg ctgtgttttc ctgtgagctc ctttctcatg
    17701 gttcgtcgga tatggaaagg agataaagca gcagatcggg gaaaggaacg ccttcccttt
    17761 ttaccctttg ttcttttggc agcggtgttt ttactttttt ggtgacagag gggaggcagg
    17821 aaaatggaaa agcatttaaa gggaagcttt accgtggaag cagccggtgt gatggcagtg
    17881 attttatttg ggattggtac gatcattaca gaggcaggca gaatccatga tcaggtatcg
    17941 tgtgcaatga ccgttcatga ggcggcagaa aaggcacgtc atgagaaaac tgtggattta
    18001 gaagaaatag aggccttttt tcagaaaaac aaaggattga ggctccacat gaaggatgac
    18061 cggctttctc tgagagaaga ggggaaatgg attcgtgcgg cagggcgggc agaaaactgg
    18121 gaaaaggaga tccggctccg acgctttcgc ccggaaacat ttctgcggaa gattacattg
    18181 atagagaaac tgggggaaga ggatgaagat cagctatatc cgtgagatga agcgcagcta
    18241 tcttaccatt gaaacagaag atgaggagca gagggaaaag ggagcctggg aggcaggaat
    18301 gctggaacag aacagaatcc ggggactgct tcccatgaga gtgcgttatg aagaaggaag
    18361 ggaaatttac tgttacgata tcacatcccg ccagccgctg gagcggatat tggaaactca
    18421 tgcaatgaca ggggagcagg tcagaagttt gttctggcag ctttaccggg ctttggagga
    18481 gatggaaaaa tatcttttag agagtggagg gattcttcta acgccggaat tgatctatgc
    18541 ggaaccagag cgttttgaaa tagggctctg tgcagttccg ggaggaaagg gagattttgc
    18601 agcagaactc agccattttc tccaatacct tttaaaacat gtggatcata gggacaagga
    18661 ctgtgtggta ttggcttatg gtttgtatca ggagagctta aaggaaaatt atgggattga
    18721 agatctggta aagattgtgg ctgaggagaa aacaggccgt ccgccgcagg aggctggtca
    18781 aaaggcggaa cggattaagg ggatacaaaa gaccggggag gatgaagtac gggatacttc
    18841 gggagcattg caaaaagcag aaggaagatc gaataaaaca gctttgccgc ggcaaacttc
    18901 cacggattta ccgcaggagg ctgccggaca gactgaatta tgcgaaaaga aaaggcagtc
    18961 tgaaaaaaat attggaaaac agcttttaaa acgtatggca gccacagcag gcatgttcag
    19021 cctgatctct ctgggaatct ggtttctgag aggaatagaa acacttttaa atctcagctt
    19081 ttatctggtc ataggcgcag ctgtggcaga ggtttttacg attttggcag tcattttctg
    19141 ggaaagaaga gaaaagaaac cggcggatac agagaaacag ataaaaaata ccagggatga
    19201 agaggatccc tggcgtattc tgtatgaaga tgaggagggg gcagaggaag aaatagaaaa
    19261 cagcagtaca gagtcttctt taagaataaa cgctgcttca atagcttccc cgcctccgga
    19321 ggatttccag actacggttt tgtccgtcca tccggaagca gaagagtgtc acggccttac
    19381 agccctccat ccggaaatat cctccataga aattccctat tatccctttg ttattgggaa
    19441 gcataaagaa ctggcagatt atgttctcga ttataaaaca gtcagtcggt ttcatctccg
    19501 ccttgaccgt acagaagaca aaattacagt cactgattta aactctacga acggaactac
    19561 cgtagcaggt gttttgctgg cggccaatga aaccgcagaa ctgaatccgg gagatgaggt
    19621 acggattgcg gatctgcggt ttgtgtggaa ataagcctgt ttttacagct tccaaccctc
    19681 cggcaggtaa gcttttgtat ttacaagcat atcgtgaacc agttcccgga tttcctgttc
    19741 ggaaggattt ttgcccttta cgatcggatc gttcataaag gcggttacta ccagttcttc
    19801 gtcacaggta agggcagcct ttaagatgcg ctcatggttt tctacatgag gcatgatcag
    19861 agctttcacg tcttcagaaa gtgcacctgc ggcaaccgga tgaatgctgt ctaagtcaaa
    19921 gagcgcattc gtttccacca gggcgttagc aggcagattg gggatctgaa gggctgtatt
    19981 ggggatgttt acattgctta caaaacgatc cagaccgcaa agggctttga ttaaaaggat
    20041 gccctcctca ccggtaggct ctaaagttac ttcttcctca gcatttgcca gtctgtggct
    20101 tctctccagg cgcttctgta aatcgttctt tctccagtcc acggtagtca ggccgaattt
    20161 ccaggaatgt acggtttctg gatcattcag gtattcgttt ccgggcataa attcagccag
    20221 atggcggtcg cctgcggcag caatgagacc gaaacggtta aagagatcca tttttacccg
    20281 gtgagcgcat ttgaaagaag aattcatcca gttttcacct tcaagttcat agccatcttc
    20341 aaagtgtttg gaaatgtagt ttttgtatac ggggaaaaga tcaacgccct tataatttgc
    20401 ttccgtaaac caggtgaagt gattgattcc tactacattt acatgaacgt cgtgccagtc
    20461 ttcaatggta tttcctgttt ctgcttcata gattcccttt aataccttct gtgtaccgaa
    20521 tacctcatgg cagcagccaa aggccttgat ctccgggaat acgtggtaaa gagtttttac
    20581 gcacagggac atagggttgg tgtagttgat aacccagcag tccggcgcat aatcccggat
    20641 ggcttctgcg atttcaacaa acatgggaat ggtgcgcagg gcacggatca tgcctccggg
    20701 gcctgcggta tcgcctacag actgatagat gccgaggcgt tcgggaagat gaacatcgga
    20761 cgccatttca tcaaaggtac ctggaaggat ggagataacg gcaaagtcac agcctgtcag
    20821 aacatcctgt aagctgtcct ttacttcaaa tttccagtca ccctttgcgt cctcccggct
    20881 cataaggtga tttccgatca ctgcgttgtt ttccgctgcc tgacggtcaa tgtcataaag
    20941 tctgatggtt ccgctgatct gatcgtccag agccagatcc gtcataaagg tccaggccca
    21001 gcctctggaa ccgcctccga tataggcaat ttgaatatcc gatactttgt tattgctgta
    21061 gttcatgatc ttaccctcct aaaaatctct gtacgaatgt ttataagtat ttgctgattt
    21121 atattttact caatggatgt ggtgaatgct aataaaatca gatgattttt taatgttatt
    21181 atctaatcgg ctacaaaaag cgtagaaaat cggaagaact tgcagtataa tagcagaaaa
    21241 ataaagtagg caggtcagaa gatgggagaa aatacagaaa aggatacgat ctatctcacc
    21301 tccgaacagt tgggagagga accggaaatt ttatttcagg aggagatttc cggtattttt
    21361 gcagaagaag cagtgcgcag gcacagtttt tccatggtaa gccgtcactt tcaccagagt
    21421 ctggagctgt atttcctttt agaaggagag cggttttact ttgtagagca ggatacctat
    21481 cacatgaagg agcagatggc gattctggtc gatcaaaacc agattcacaa aacctgcccg
    21541 gcaggaaacg gaaaaagtca tcaccggttt cttttgcagc tagagggaga agctttaagc
    21601 tgccttttga aactttgcgg tttccgggga cttttggagt ttggagagca gtatcgggga
    21661 attacctgtt tttcggacgg cgaatggaaa caggtgctgg agcagctgga aaagataaaa
    21721 aaagaaatga gtatgtccca aagtgcaccc ggatggtcag atacggaaaa gagaacggcg
    21781 gaggggatgg tgcggctgta tacggcccag ctgcttctgc ttctgatacg aaccagaagc
    21841 agagcagagg cggaaggatg gaaggagcca ggacagacca atatagttca tacgggaatg
    21901 tatcagaagg ttcatgaaat tgcgatttat ctgcagaatc attccgcagg aaaactctgc
    21961 ctagatgatc tggcagccca tttttttatc agccgttcct atttgacccg gattttccga
    22021 acagttacag gttttacagt caatgaatat caaaatatct gccgcattaa aaaggcccag
    22081 attctgctca gggagacgtc attgactatg acagagatag caatggaaac aggatttgga
    22141 agccttccct attttgaacg ggtattccgc aaggctaccg ctcagactcc tatgcagtat
    22201 aaaaaatctc acccttcacc gtgaatgcgg gctatatagg aactgggggt catgccggtt
    22261 tttttcttaa acagctgaga aaaatattgc ggactgtttc cgcagcccac ctgctctgct
    22321 atggtctgaa tcttatcgct ttcggcagag acaagcagct gtttggcccg ttctattcgg
    22381 acatcggtga gatagctgga aaaacgggtg ccagtttcct tttgaaattt tttactgaca
    22441 tagtctgtgt tcataaacag atgctgctct gcaatccgct ttagggtcag gttgggatca
    22501 gagaggtgtt catttacata gtcgcagatc tgctgggtca tggcgctgat ggaggaagtc
    22561 tgaggcttag ccgataagag ctttctcagc ttttctgcaa gggtaagctt aagctgctct
    22621 aaattccgtt ctctgtttac atttaaaagc cattccgtca gatcaatggt ggaaagacgg
    22681 ttgtcagaag ctgtgatttt cagcaacagg gaattggaaa gctgcttgag aaatgacaga
    22741 tcagttactt ctgaaagcaa gttaatcaga ctgtcggcag actctgttcg tccagtttgc
    22801 aaagcctctg acagctgttt ttccgcttct tccataatga agctgtagtt gcagctggat
    22861 aggatgcgga agtcattgat atagtagatc atactgaaac gttttacttt atctaaaaca
    22921 gtttccagaa gagtctgaag atttttaaag gacaaatcct ctgtttcaag ggaagggctg
    22981 cgtccgggga attttatgct ggaaaaaaat tgggacagat cctgataatt tcctgcaaat
    23041 tccttaaaaa acagcagcag cgtacagttg acgtagatgc catgtatggt aacttcggga
    23101 aaatggctgt cagcataggg tttaagctgc tgtaaaaact cctccagatc cttctgttcc
    23161 agaaaataga tatagaaaag atgataggat gaaaaataaa aatccatata cggatcatag
    23221 gcgcgcataa tttcatcata aggacggttt tggcagatgg cattgttaag gattgtaaaa
    23281 ataacattgt gccttatact gctcatagcg ggataggagc gttccagact gctttttcgg
    23341 taacagtcct gagcaatttc ctgtatgctt tcaagaatct gcacttcact gcagggcttg
    23401 agcagataat ggcggactcc atatttcatg gccatttttg catattcaaa ttctccataa
    23461 ccagagagga taatgaactg ggtgttcaga tctgtttcgg aaatccgacg aatcagctct
    23521 agaccgtcca ttccgggcat tcttatatca gtcaatacaa tatccggcgt ttcatccagg
    23581 attgtatcat aggcttccag accatttttg caaaggccta ccagttcaat atcatattgc
    23641 ttccaattga taattgtgga aatggtttca cgtatgatgc gttcatcatc agcaataata
    23701 agttttaaca tgagacagtt tcctccgtgc gatatggaat agtaatggat gctacggcgc
    23761 agtcttcctg attaaacagg gacaaaccat agtcttcacc aaataacagt ttaatacgtt
    23821 tgtcaatatt taaaagacca attccggaac ggctgggctg ttttttatgc tgtttaagca
    23881 gttccaggag atcttcatca aagcttgagc cgctgttcgt tacagagatg cggatacagc
    23941 gctccccgtt ttttcctgtt ttatctgttt ctgcagctaa aatggtaata tagcaggttt
    24001 cagtcatttc ttctaaggca tagtgtatgg cgttttctac cagaggctgc agcgtaaggg
    24061 gaggaatcaa ggcatttttc agtccatcgg gaacctgttc ccggtagtcc aggcgttcct
    24121 caaaacggat ctgctggatc gtgatatagg acagaatcag attaagctca taggacagtg
    24181 taacaagaga ttttttgttg cttaaagtag ctctcagaag agagccaagg gattctgtca
    24241 taagagaaat ctcccgattg ttgaccgcct ttgcccgcca gttgatggat tccagcgtat
    24301 tgtagagaaa atggggattg atctgggatt ctaaggcttt aatctgtgcc tcttttgcaa
    24361 ggatttcatt aacataattt gtatttacca gattttggat tcgtgcggtc atacgttcaa
    24421 aaccctgatg gagccgcccg atttcatcct tgcggtaggc atattcggaa tcgtattttg
    24481 tacttaaaag cgctgcttca tcccggctga atttgtccat ttttttcagc agaattttaa
    24541 tatgttttaa aatagagcgg atcaggcaat aggaaataaa aacaatcagg acaacgcctg
    24601 caaacaggat aaaaagaata aatgccgcag aggtgtgaag ggtatgtatg atttcatcat
    24661 aggaaaccag gctgtagtat tccagtccgt aaccgggaat cgtattatgc actgtaaaat
    24721 aggtatgaga atccagtttt aacgtttggt atttttctga agaggaagaa atgatcttgt
    24781 ccgcaagaga atcagaaaat tcttccggca catagatttt ctggtctttg ctgcaaagaa
    24841 tatactggct gtcggagtat tgagtcgcag cagcgtttgc cttgcggata atattattta
    24901 aatctacata aaccaccaga gttcccagat gcttcagaga aagattatct accttccgga
    24961 tttcgcggct catgaggagg gcattgcttc cgggatcagc ggggatccat accacagcgc
    25021 cctttttttg ttcagccacc tcgatagcct cctgaaggcg ctcttcggaa agcttgcttt
    25081 tccacatggt ataagtagaa ttatggaaat gttcattttg gatcatcaga aaagatgcgc
    25141 cgttgtcttt tacagaggaa tgataggtct gaagagaatt gatgatgacc cggttggcgt
    25201 ttgtccaggc ggcccggtca ttgagctctg caatagcaga gagagaaatt tggatttctg
    25261 gagcagcaag gatggtagag gatacgtttt caatactttt caggctgtca gaaatcgtat
    25321 aggctgagtg ggtcagattg ccggctgtgg tcttataaag aagttcattg taggctcctg
    25381 tgtataggcg tagaccaaag agtgagagaa ccataatgag aaatacatta tttaacacaa
    25441 taaaaagtat cttctgaaat aaaggaagat tgtttaatcg ataccggatt gaacgggctg
    25501 caaaggatat aaaaccgtgg gaaccatagc tcataaggga tgcctttcct gtaaaagatg
    25561 tgtaattctg taacaggatt tagtatacat ataaatcaga agttatgcaa tcaatacaga
    25621 aaacaggtct gttttttgaa cacgaatatc ggttttacct atcacggaat cggaaaaggg
    25681 aggagtataa taaaatcagc agttgaaacg aagcttaaag gtgcagacag cggatgcaca
    25741 ataaaacagg agggtatgtc ttatgaaaaa acgaaacatt gcactggtga tggcaggtct
    25801 gatggccgca atgagtctga ctgggtgttc aggaggagcg caagaagagg cttcggctga
    25861 tggagcagcc aagacagaag cagccggttc tgaagcagcg gatggtggtg aaaagagcct
    25921 gactgttgca tggtggggca atcaggtccg caatgaaaga acgcaggcag cattagataa
    25981 atatacagag ctgaatccag gagttacact ggacggacag ttttctgaat ggggtgatta
    26041 ctggaataaa ctggcaactg catctgcagg aaattccctt ccggatgtag ttcagatgga
    26101 ttatgcatat ctggatcagt atgcaaagaa taacttgctt ttagacctga ctccttatgt
    26161 ggaagatggt actctgaacc tggatgatgc gaatgcagat gttttaaatt ccggtaaggt
    26221 agacggaaag ctttatgccg tacccattgg cattaacgca ccggccctgt tgtacaacaa
    26281 ggcagtaaca gatgcggcag gtgttacgat caaggataat atgactctgg atgagtttat
    26341 tgaggtctgc aaaactgtta aggaaaaaac aggctaccgt accaatatgg catacggcgt
    26401 aacgcaggaa atcgtccagt acatgattcg tgcaaacggt cattctatgt atggtgaagg
    26461 caaattaaat gtggaaagcg cagatgaatt tgtacccatt tttaaggtat ttgaggatgg
    26521 cgtaaaggaa ggctggcatg tagatccgag catctacgct gagattaccc caggctctgt
    26581 agaacaggat cctttcgtat atggtgcaag tgaagattcc agatcctggt gtgtattctg
    26641 ctggtctaat cagattgggg catatgagaa ctgcaagccg gaggatatgc ctgaaatcgg
    26701 cattactacc tggccttctg ctgatccggc taaatcggat ttcctgaagc ctgctcagtt
    26761 tttctctgta tctgccaatg cagcaaatcc ggaggaagca gtaaagatta tcgactactg
    26821 gaccaacagt atggatgcaa acaatattct tttggcagaa cgcggagtgc cgctgtctga
    26881 gaaggtagct gccgaactgt ctccaagcct tgatcctgcg attcaggaaa gtattaagtt
    26941 tatcaatgag gttgtatcac ctgcttcttc acagattgat cctccaatgc caaacggctc
    27001 cagcgaggtt acagagcttt tgctgcagtt agaggagaag gtggcatatg gccagatgac
    27061 atcagaggag gcagcacagg agctgtttac taaaggaaat gagattatgg cttccaaata
    27121 ataaatttca ttttcagaaa tagaaataaa acaggtttct tagggttctg cctgctgcgg
    27181 ttataaacgt atgcaggcag aagtttttag aggaggaaat aggatgaatc cattcaaaaa
    27241 agccttaagc aaagaaagta cggcaggtat tattttcacc cttccattta ccattggatt
    27301 tttgctgttt atgatcgtac caatgggaat atccctttac tactcattct gcaaatatga
    27361 tattctttct ccgcctgtat ttacaggact ggataatttt gtgaagatgt ttcaggatga
    27421 gaccttttat aaagcaatcg gcgttacctt ttattttgca ctggtatctg tgcctttaag
    27481 actggtcttt gcgctgatcg tagcaatgct gctgttaaaa aattcaaaga tgagcggctt
    27541 ttatcgtgca gcctactacc ttccatctat tatcggaggt tccgtagctg tggcgattct
    27601 gtggaagaga atgtttgcta cggatggtgt agttaaccgt ctgctgggcg ctgtaggaat
    27661 tgaaactacc tttgcatggc tgggaaatac aaaaactgcc atctgggttt tgattctttt
    27721 ggctgtatgg cagtttggat cctccatgct gattttttta tctgcgttaa agcagatttc
    27781 aaaggagctt tatgaggcgg cagaggtaga tggagccaat ggaatccaga agtttttcag
    27841 gattaccctt cctcttctta ctcctaccat tttctttaat ctggtgatgc agatgatcaa
    27901 tggatttctg gcatttactc agagctacat tatcacccag ggaaagccca tgaattccac
    27961 gctgttttac accgtttata tgtatcagca atcctttgag ttttacaata ccggctacgg
    28021 tgctgcttta gcctgggtaa tgcttgctat tatcggagct attacactgc tgctgtttgc
    28081 aaccaaaaaa ttctgggtat atgaggaggg ttaagcaatg aaagagaaaa gagcagtcaa
    28141 agcggttatc tatcatatac tggtatttgc tgttggcctg gttatgattt atcctctgat
    28201 ctggatggta atgagctcct ttaaaccaac caataccatt tttcagacag caggaagcct
    28261 gattccggaa acatttacct ttgaaaatta tataaatggc tggaaaggct ttgcgaaggt
    28321 aacattcgcc acatttttta aaaattccct gtttatttca gtagtggcta ccattggaac
    28381 agtgatctct tctgccatcg ttgcttacgg atttgccaga tttaagttta aggggaaaaa
    28441 gcttctgttt tccgccatgc ttttatccat gatgcttccg gcccaggttc ttatgatccc
    28501 tcagtatctg tggtatcaga aacttaactg ggtgggcagc tatatgccgc tgatcgttcc
    28561 ttatttcttt gctatccagg gctttttcgt atacctgatt tctaacttta tttcaggaat
    28621 cccaagagat ttggatgagg ccgcaaagat tgacggctgt tcttatgtat ctatttttac
    28681 aaagatcatc ctgcctctga ttaaaccggc tcttgttaca gctgggattt tctcctttat
    28741 gtggagatgg gatgatttcc tttccgcact gctttatgta aataagtcag ccaagtatcc
    28801 ggtgagtttg gcattaaaac tgttctgcga tcccggctca tcctccgact atggtgccat
    28861 gtttgccatg gcctctcttt ctattcttcc ttccgttctg atttttattt tcttccagag
    28921 atatctggta gagggaatca gcacatctgg actaaaggga tgataaaaga gcaatatagt
    28981 gatagaaaaa gagaaaatac cgtattaaaa tggacaaaaa ccgaggtata gacacaaaat
    29041 tgcccggtac agaaaggaaa ggaaaatgaa actgaactat gataaagctc agcttgaggc
    29101 ggttgtagac aaagtggcag agcgcaccat gaacatggat atgacctggg actggccctg
    29161 cggtgtagct tattacggca taacggaggc ctatgaagcg acaggaaata agaagtacct
    29221 tgatatggta aaagagaggg tagatgagct gatcagcctg gggcttccgg actttacggt
    29281 aaatacctgc gccatgggtc atgtgcttct gacgctctat aaggaaacgg gtgatgagac
    29341 ttataaaaag atcatagacg ataagctgga ttacctcaca aaccatgctc tgcgctttgg
    29401 ggatcatgtt cttcagcaca cagtatctgc aaataatgat tttccggagc agtgctgggc
    29461 agataccctg tttatgtctg catttttcat gctgcgggca ggcgttatgt ttgaacgtcc
    29521 agaactggtg gaggatgctt taaaccagtg gtactggcat atccagtatc tgcagaatga
    29581 gcgcaccggc ctctggtatc atggatatga caatattgca aaggatcaca tgagcggatt
    29641 ttactgggga agagccaact gctgggctgc ttttaccatg tctcaggtgg ctcatatcct
    29701 tcctcagtgc tatctctatc caaagtatct ggaaatagta ggaagtttaa atgaacagct
    29761 ggctgtttta aagacgctgc agacggaaaa tggcctctgg agaaccattc tggatgatcc
    29821 ggagtcctat gaggaggttt ctgcctctgc cggaatcgca gcagctatgg tgttaaaggg
    29881 aaatcctctt catattaaat atattaacaa agccatcaca ggaattttgg aaaatatttc
    29941 tgatgatgga aaggtaatga atgtatccgg cggaacagca gtgatgaagg acagagaggg
    30001 ctaccggagc atttccaaac gctggattca gggatggggc cagggaatgg cgctggcatt
    30061 cttcagtacg gttcttaatt atgaaaaatt tacaggcgac ggagcgctgt aggagggaga
    30121 gtgccaaagg gcaggcagag aaaatggaac atacaaaata tccgcataca aagggcagct
    30181 ttacacttcc gggagaggca ggctgcgagg ctttgacact ggagctggca gatcaatggg
    30241 gggcagatgt gatccgtgat tcagacggaa ccaggctttc agatgaaatc gtaaatgcag
    30301 gctatgggat ctattccacg atctgcatca tccgggacca caatgaatgg gccagagccc
    30361 atatggaccg tctgcagcag acatttctca tgtctgctcc caaaactgca aaagcagata
    30421 cagtaataat cggccttctg gacggctatt ttaaggagca gtttcttgta aatggttcag
    30481 aggattctct ggaattctgg caggtgtttg accgcacgga caatgcagag gttcccagag
    30541 agcagtggac ctttgatgag gcgcagggca ctgtgacggt tgcaggaatc agggcatatc
    30601 acgattatac ggtgaatttc ctggcatatc ggatctggga agagatcaat atgtataatc
    30661 atgttacaaa tcactgggat aaggagcatc tgatgcccgt tgatccccgt tacccggata
    30721 cgcaggaata tctgctttcc tggctgaaaa actggtgcgg ggaaaatccg gataccacgg
    30781 tggtacgttt tacctctctt ttctacaact tcgtttggat ctggggagag gatgaacgcc
    30841 atagaaacca tttcacagac tggggctcct atgacttcac cgtaagcccc agggctttga
    30901 aggagtttga gaaggaatac ggctatggcc tgacctctga ggattttatc aatcagggac
    30961 gccttcacac gactcatatg gctcccggca aaaagcagct ggattatatg aaattcacca
    31021 accgttttgt gatttctttt ggaaagcagc tggtagatct ggttcattcc tatggaaaaa
    31081 aggcctatgt gttttatgat gacagctggg taggactgga gccttatttc ccaacattta
    31141 cagaatttgg atttgacggt atcatcaaat gtatcttttc aggctatgag gtccgcctgt
    31201 gtgcaggtgt ggacgctccg gttcatgagg ttcgccttca tccctatctg ttccctgtgg
    31261 gacttggagg ggcgcctacc ttttccggaa atggagatcc caaaagagat gctgaaaaat
    31321 actggattgc ggcaagaaga gccatgctcc gggcaaaaat tgaccggatc ggtttgggag
    31381 ggtacctgca tctgttagag gattatcctg attttgtgga atatatcagc cggactgcag
    31441 atgaattcag agccataaag gctcttcatg aggagggaga gccccagctt ctgcgccttc
    31501 atgtgggagt gctgactgcc tggggaaaaa cacgctcctg gacactttcc ggccatttcc
    31561 atgagacctg gcagaatgat ctgatacata tcaatgaggc attgtccggc tggccggtaa
    31621 aggtggatta cattgatttt gaggatataa gggagcagga gctttctgac tatgatgtaa
    31681 ttatcaatgc aggctttgca ggaagcgcat ggagcggcgg agagcagtgg aacgacagcc
    31741 gggtactgga aaaactcaga agctatgcag aggagggcgg atgtatcctg ggagtatgcc
    31801 agccttctat gataaaagga tatatgacga atttccgtct tgcagatgtg ctgggcgtag
    31861 atcaggatac gggcgaacgg tgctgtcatg ggcggatttc ttataaaaag gcagagaaat
    31921 tctttggaga tgacagtgta ttcagcaaaa aagagcttga ttcgattgca ggaagcctga
    31981 aaggaacgga aggtatttat cttacagatt ttaatgtaca ggtgttggcg gaggatgtgg
    32041 cctacagccc gattggagaa gaggtacgta gccctaagct gacctatcac ccttatggaa
    32101 agggctgcgg catttacctg tcacatttta catacagcgt tgaaaacagc cgttttcttc
    32161 tggaactgct cttaaaggca gcgggaaagg aagaggcaaa agcgcagctg ggagataaca
    32221 tatattgtga agcagcttat ttcccctgtt caggaaaggc agtggcagtc aataattctg
    32281 atcggcctca gcacttgaag atcaccctgg gcggtgccgt aaaggaagtg gatctggagc
    32341 cctgcggcag ctgcatgata gaaatataat cataaaaaag cgcaggtaaa agagatggca
    32401 gagtacagat ttacagatca atgctttgaa aatgctacgg aaaaatgacc agaagcgcag
    32461 gaatttaaag gcctatgatc agtttgcgtc caatgttcgc cgctatgtaa tggaaaccag
    32521 gaggctgggc gctgtaccgg tgcttgttac ccctatgagc cggattccgg ttcgggacga
    32581 aaagggctgg tatgatctgc tggaggctca tgcagacagt atccgacgtg tggggcagga
    32641 gatgaacgtt cctgtgattg accttcacag cctgactttt gaagcattct gttccatggg
    32701 gccggagacc tgccagaatt attttaatga taccacccat gtaaatgatt acggcggagc
    32761 tcttctggcc gatatgatgg taaaagaaat acggaggctg agaattgagc cgctgtgcag
    32821 tcatatgaac cataccgttt ctggtggctg ggaaccggat ctgtcccttc gtcctcaggg
    32881 acaggcagaa agcagcaaga tcgaggagcg gcctaatctg agcatggatc tgccggagct
    32941 cccctatgag gactgccgca gcctgaccaa aaaggatgtg gaaatgctca agcaggcgat
    33001 gaaatgcggt cttttagatc cctgcgtgcg atatctccat cccttggagg aaatgccacg
    33061 ggcgcagttt gtgtttctgt tcctgaaagc agtgaaacca acgctcagac gcccctggca
    33121 gggtgagtat tgtgacttaa gccgttatga gtatgatgcg gagcagattc aggcggcctg
    33181 ggatgaaaac cttattgatc cagagactac accggatgat cgcttccgtc cggatgatcc
    33241 gctgacactg ggagaactca taagctttac tgtcagggca ttccgcccca aacagcagag
    33301 aaggattgga agtctggaat gcatccggga ggcagaaaaa ctgggatgga tcaaagatac
    33361 ctgccaggat atgaaccgag ttgtaaccag agcagaggca atccaaatga cggtaaattt
    33421 gtactgtgaa aaatttacat aattcttcct taattgggcg atttttacag gggatgggga
    33481 gaattttcca tattgggaat gcaacccact taacaagaga ttaaagttat gttaatatta
    33541 agcatatctg gaaatgattg taaacgacat tgaaaatgga taatcattct ggatatgctt
    33601 ttatcgtatg gtatgaaaag cggttcgggc cgcaggaggg aggaatgtca aaatgacaga
    33661 cgataagtgt attttctgca aaattgcaaa cggagagata ccgtccgcaa cagtatatga
    33721 agatgatatg tttcgggtta ttctggatct gggaccggcg accagaggcc atgccctgat
    33781 tctgcccaag aatcattttg cggatgtatg cgcactggat gagaaaacag ctgcgaaagt
    33841 gcttgctata ggagcaaagg tcggcgcagc gatgaaagaa ggattaggct gtgcaggttt
    33901 taacctggtt cagaataatg gagaggcagc cggacagacc gtgatgcatt ttcatatgca
    33961 tgtgatcccc cgctatgagg ggggacccaa tatagtcagc tggacacccg gagctgtttc
    34021 cggagatgat cttacagagc tggcagaaaa aataaaaggc agttttcgaa agcaggaatg
    34081 attaagaaga tggatgaaaa agccagaagg cggtttgagc agatgtacca ggagtatgaa
    34141 ggtaccgtcc gcagacttgc ctatggatat aatattcctg tagattatat agacgacatc
    34201 gttcaggata catttgtttc ctatgcccag tatgattatt ctctggagca gacagaaacg
    34261 ggcaagagga tacttcttgg ccggattgtg aaaagccgtt gtgtagatta ccgcagaagg
    34321 cttgaaaaca gaacctgtga ggatgtagat aatgatgatt ttataatgga agacttcagg
    34381 atacagaata gaaagcagag tattccggag tatgtagcca gtaaagagcg atgtatcgcc
    34441 atactggatg caattgagaa tatgcctgat aactggcaga aggtggcaaa tttaaaactg
    34501 atacagggaa ggcccacaga ggaggtatgc cagatcctga atatttcaga aaaggcctgc
    34561 tactccagag tgagccgtat cagaaagtat atggaaaccc ttctcggaaa tgaaatgtgg
    34621 ccgtagaagc gattattttc cttcggaaca ttcattgatc agcgtcatga tagtttccac
    34681 tgcatggttt aatttacttg tttccatggc gctgatatag gaggaacgat tggcataaag
    34741 ttcagttact gcttcacaga gactatcatc tgtgatgact tcctcctcta aaaccttgct
    34801 gaagccctgc tttgcaaagg aatttgcatt taagatctga tcgccccggc tggcagctgc
    34861 ggacagcggg atcagcagat tgggcttgcg gagggctaaa agctcgcaga tggagttggc
    34921 tccggctctg gaaataacga tatctgccgc tgcaaagagg tgttttaacg gagcgtctac
    34981 atactcatac tgcacatatc cctcggtgcc gatgagagat tcatctagat tgcccttacc
    35041 gcagatatgt atgatctgga aagagggcag aagacggggc agtatacggc gcacagcggt
    35101 gttgacagcc acagacccaa gactgcctcc tatgatcagc aggacaggtt tctcttcttt
    35161 taagtgcgca taagccagtc cggaagtccg ttctcccttt aaaagctctg cgcggatggg
    35221 agagccggtg agaactgcct tgtcggcggg aagatatttc atggtttcgg gaaaattgca
    35281 gcaaaccctt gcggcagagg ggatgcagat tttatttgcc agtcccggcg tcatatcaga
    35341 ttcatggata atggtgggga ttttatagtg tttcgcggcc agtacaaccg gaacggctac
    35401 aaatccgcct tttgaaaata caacatccgg cttatagcgt tttaagaggc gttttgcctc
    35461 ggcatagccc ttgatcacac ggaagggatc tgaaaaattt ttcaggtcaa aataccggcg
    35521 cagcttaccg gaggagattc cgtcataagg aatgcctgca ttttcaataa gttttcgctc
    35581 tattccctga taagaaccga tataacggat ctcatacccg gcttccttaa gagatgggag
    35641 cagggccagg tttggagtga catggccggc agtgccaccg ccggtcagaa tgattttttt
    35701 cataattgac ctcctgaatg agtatctgta acagcacgga aaacagaggt gctatagtat
    35761 tatagtaagc gttaactgca aaaagtcaac cctaaagcat cagggaactt aaggagattt
    35821 ggcaaacaga ggtgacccgt ggaaaaattt aacagcaaaa aaccagatca gaatgataat
    35881 atgcagagag agccgggaga aataagcggt ttttcagatg aacaggtttt aagggagttt
    35941 gaggaggcta tgaaaagtac gatccccctg cctcccccac cggaaaatga atttgaacag
    36001 atctgccagc ggatagaaaa ggaaaataga atggaacaga ttgacggatt tttgtgaaat
    36061 atcattttca gtgcaagaaa ggacacaccc catgcaaaac ctaaaagaaa aatttcagcg
    36121 ttttatgatt ggccgatacg gcgcagacca gctgggaaga tttctgtcga ttgtaattgt
    36181 catattggca gcagtaagct tctttatacg ttcctactgg tttggctcat tgatccgcag
    36241 tgtgctgatc atccttttgg caattcttta ttttcggatt ttttcccgga acattaccag
    36301 gcgcagtcag gaaaataatg cgtatatccg cctgcatttt caggtttcag agcgcttaaa
    36361 aggctggcgc ttccatctgg aggagagacg cagatataag attttcagct gtccaggctg
    36421 taaacagaag atacgtattc ccagaaatca tgggaaggtc agcatccgct gtcccaaatg
    36481 cggccatgat tttataaaaa acacaggcgc gaaaaaatag actgtagtac cggaaaagca
    36541 aagactgctt ttgccggtat ttttttgtta tttttccata aaacgcgtta gtttacatag
    36601 aatttacaaa acttatacct ttccttgctt tctctcaatg taaaactttt gtatactcct
    36661 ccatgtagcg aagaacacaa cacaatgaaa ataaagcgca gagcgtaacg tggaggaaag
    36721 aatattatga aaaagaacag caaaatgatt ctggcagcag ttggtacagc agtgatggca
    36781 gcaggtatgc tggcaggctg cggaagttct tctaaagaga caacagcggc acagacaaca
    36841 acagcggcac agacaacaac agtggcagac agcaaggctg ctgataccac ggaagcaaag
    36901 aaagaggaga aaacagaggc tgctgctgat ttaagcggaa ccatcagcat ggtaggttct
    36961 acttctatgg agaagttcgc aaatgcactc agtgaaacat tcatggaaaa atatcctaag
    37021 gtcactgtga cggcagagtt tgtaggatcc ggtgcaggcg ttgaggcagc tgcaaatgga
    37081 acagcagata tcggaaacgc ttccaggaac ttaaaagacg aggaaaaagc aaagggaatc
    37141 gctgagaata tagtagcaat cgacggtatt gcagtggttg ttgatccttc taatacggta
    37201 gaagatctga caaaggatca gctggccggc atttatgacg gaacagtaac caactggaag
    37261 gatgtaggcg gaaatgacgc tccgatcgta gtcgtaggcc gtgagtccgg ttccggtacc
    37321 agaaccgctt ttgaagaact gttagagctg gaagataagt gcaaatatgc aaatgagctg
    37381 gacagcacag gagctgtcat ggcaaaggtt gcttctactc ccggagccgt aggctatgtg
    37441 tccttagacg tactggatga tacggtaaaa gcagtaaagc tggagggcgc agagcctaca
    37501 gaagaaaaca tcaaggctgg aacctacttc ttaagccgtc ccttcgtaat ggctaccaag
    37561 ggtgagattt ctgagcagaa tgatctggta aaggctctgt ttgatttcat ttattctgag
    37621 gaaggcgctg aacttgtaaa gtctgtagga ctgatttctg tagagaaata atccgggctg
    37681 gaaaggagac gatctgcaat gacaacggaa acctgttcca tacgttccca tcatggcagc
    37741 aagtccggcg tagaaaaagc tgcacaggtg attttcaccg cctgcggctt tttcgctgtt
    37801 ctggctgttg catccattac actgtatatg attatcagcg gcactcccgc cctgtttcag
    37861 gtgggtctta cagatatcct gttcggttct gtgtggaagc cggatgccgc agatcccagc
    37921 tttggtatct tatatgttat cctgacttct attgtgggaa ccttccttgc gatcctgatc
    37981 ggagttccta ttggagtgat gaccgctgtg tttctggcgg aaatcgcacc gaaaaagatt
    38041 gcatccgtag tgcgcccggc agtagaactt ctggctggaa ttccgtctgt tatttatgga
    38101 cttcttggta ttctgatttt aaatccgctg atgtataagc tggagctgat catttttaag
    38161 ggaacagatc accagtttac cggaggagca aacctgattt cagcagtgct ggtgctggct
    38221 ctgatgattc ttcctacagt gatcaacatc agcgagtctg ctctgagagc tgtgcctctg
    38281 cacttcagaa gcgcttctct ggcaatgggc gctaccagga tccagacaat tttccatgtg
    38341 gtgcttccgg cggccaagtc aggtattatc acagctattg tactgggaac gggccgcgcc
    38401 attggagaag ctatggccat cagtctggta tccggaagct cggtgaacct tccgctgccc
    38461 tttaattctg tccgtttcct gacaactgct attgtggctg aaatgggata cgcttccggt
    38521 ctccacaggc aggtgctttt taccattgga cttgtacttt ttgtatttat tatgctgatc
    38581 aatatcagtc ttacaggaat tcttaagaag ggagataaga agaatgacag gtgaagcagc
    38641 cgtcaggctt aataaatatt ctgaagttag agaaagcatt tacggaaagc atgtccgttc
    38701 gtcagatatg ctgctcacag gtcttattta tggctgtacc gctgtttctc tgcttattct
    38761 tgtgggcatt ctgggatata catttgtaag agggattcct catgtgacct ggtctttctt
    38821 aagcactgct tccagtgcgg tcaagggaac ctttggtatt ctgggaaata ttattaatac
    38881 gatctacata gtggtaatta cactgttgat cgctactccc atcggcgtag gttctgccgt
    38941 ttatttgaat gaatatgcaa agccggggaa gattgtccgg atgatcgaat ttacgacaga
    39001 aaccctttcg ggtattccct ccattatctt tggacttttt ggaatggtat tttttggaaa
    39061 taccattggt ctgggttatt ccattcttac cggttccctg acgctgacat tgatgatcct
    39121 tcccctgatc acgagaacga cacaggaggc tttaaagacg gtgccggaca gttaccgtca
    39181 tggcgctctt gggatcggag ctaccaaatg gtacatgatc cgtacgattc ttcttcccag
    39241 cgctatgccg ggtattgtta cgggcgtgat cctggctatc ggacgtattg taggagaatc
    39301 tgcggctctt ttattcactg caggaagcgg ttactatctg cccaaaaata ttcttggcaa
    39361 gatttttgag tccggaggaa cgctgacgat tcagctgtat ctgtttatgc agaaggcaaa
    39421 atataatgag gcatttggag tagctgtggt acttctggta atcgttctgg ttattaacgg
    39481 tcttgcaaaa tatttgtccc atcgctttaa tgtggaaacc cgataagcgc cggaggcagt
    39541 caaaaggaga aaaactgtgg aaaacaaagt aaaaatctcc gcacagaatc taaatctgta
    39601 ctatggagaa aatcatgctt taaaaaatat taatctggat ttatatgaaa ataagattac
    39661 agcctttatc gggccttccg gctgcggaaa atcaaccttt ttaaaaacat taaacagaat
    39721 gaacgatctg gttcccaatg taaaaatcga gggaacggtt cttctggatg atgaaaatat
    39781 ttatgatccc caggtagata cgactctttt gcgcaaaaag gtgggaatgg tttttcagca
    39841 gcccaatcca tttccaatga gtatctatga caatattgct tatggcccca ggatccacgg
    39901 gattaaaaat aaggcccaac tggacgagat agtggagaga agtctgcgtg gggcggctct
    39961 ctgggatgag gtttcagacc gtttaaagaa atctgctctg ggactgtctg gcggccagca
    40021 gcagcgtctg tgcattgcaa gggcattggc ggtagagccg gaggttcttc ttatggatga
    40081 gcctacatct gccctggatc ccatttccac gttgaagatc gaggatctga tggatgattt
    40141 aaagagcaga tatacagtgg ctattgtaac tcataatatg cagcaggcgg cccgtattgc
    40201 agattataca gcctttttcc ttgtaggaga ggtggtagaa tatgcttcta caaacgaatt
    40261 gtttaccatg ccaaaggata agcgtacaga ggactatatt acaggacgtt tcggctgata
    40321 gacagtaaaa ccgcggcgga aaggaaaaga tatgacagtt cgcataactt atgaacatga
    40381 gttggaagag ttgaataagg atttaagaga aatggcgttc atggtggaaa aggccattga
    40441 gcagaccttt gtggcttttg aggatcagaa ttatacactt gcagaagatg tcatcaaggg
    40501 ggatcggacg gttaatgata tggagcgggc tatagaatcc cgctgcctgt ctctgatcct
    40561 gcgtcaacaa cccgtagcct gcgacctgcg ccagatttcc actgctttaa aggtggttac
    40621 ggatctggag cgtatcggag atcatgcttc cgatatagca gagctgatct tgaggattaa
    40681 aagcgaacac gcttatcaca ttgtaaagca tcttcctctt atggcggaag aagccaggaa
    40741 aatggttcac gatgcggtag aggcctttat ttcccaggat ctggaagaaa ctgaaaaaat
    40801 catccgtatg gatgatcgag tagacagcct gtttaatcag gtaaagacag atgtgatcag
    40861 cctgctgaaa acatcgccgg atcaggcaga tcagggaatc gatctattga tggtcgccaa
    40921 atatctggag cgcatcggag accatgccgt aaatgtgtgc gaatggaccg agttttcaaa
    40981 gacaggcgct ttgaagcatg tgcggatcat gtaacagaaa ctgttttaat atgaaaaatg
    41041 ccagccccgc aagcgaaaac ggagctggca tttttcatat cattattcgc caaacacagc
    41101 cttataatct gcctggaatt ttgcaattcc ctgatcggta aggggatgct ttgtcatctg
    41161 ctccagcacc ttgtagggaa cggtagcgat atctgctccg gctttggcgc agtcgatcac
    41221 atggatcgga ttgcgcacgc tggctgcaat gatctctgtt tcgatattgt gcatctggaa
    41281 aatctccgtg atatcgtaga tcaggtcaat gcccggcatg gagatatcat ccagacggcc
    41341 taagaaagga gatacataag tcgctcctgc tctggcggcc agcagtgcct gtgcagcaga
    41401 aaataccaga gtaacatttg ttttaacgcc ttctgcggaa agcaccttta cagcctttaa
    41461 gccttctacg gtcatgggga tttttacaac catgtttgga tggatggcag cgatctcgcg
    41521 gccttctgcg atcatgcctt ctgcgtctgt ggttgtagct tttacctcgc cgctgatagg
    41581 gccgtctacg atggatgtga tctcagagat tacttcctta aagtcgcggc cttctttggc
    41641 gatcagagac ggattggttg taactccgca gatgatcccc atatcgtttg ccttgcggat
    41701 gtcttctaca ttagcagtat caacgaaaaa tctcataaac aaaatcctcc tcaaattgct
    41761 gtatttcata ctggtttata catttattat agcgccatac agcacaatgt caagcataat
    41821 atcgcaaaaa aatgcgttaa tttgcgcaaa ataaaatcat gtgaatacat aaattattaa
    41881 aaacgcaaac taacgcatga attaaagaaa aactaacgca aacaaacgca aaaagagcaa
    41941 agagaggctt tgcaggccgt ttttatataa aatccagtcc ccggttccgg tatcccaaag
    42001 ccatctcgcc ttccacagat ccgtctgtaa taagggctgt aaaggcttca atcccgcaca
    42061 catgatatcg tgatacggta ttcagcttca cgggatccac cagagcgatg acccgtctgg
    42121 aggatcggat cagctggctc ttgatactta cctcttcagg atagggaatc gtcatgccgt
    42181 attcggggtg tacggcataa acgcccataa aacacagatc cggatggtag gcggctgctt
    42241 gagcagctgc cgtttcacct acggttgtca tggaatcctt taacagatcc ccgcccagaa
    42301 cgatcacctt tacctgctcc ttcatggaac acacatgggc gatgctgtag ctgtttgtta
    42361 aaatcgtaag tttcatatcg gaagggatat tcttagcaaa ctgcagattg gtgctgctgc
    42421 cgtcaatggc taccagctgg tcgggctcca gaaagcccag ggctctttcg gcgagcttct
    42481 gcttcagctc tacgtctgcc tcttcccggt cgaaaaaacc gatgaccgga cgttccagag
    42541 ggattgcccc tccaaacacc cgttttagca gtcctttttg atctagatcc agcagatctc
    42601 tgcggactgt atcctcggaa accttaaggt cggctgccag ctggcttacc acaacctttc
    42661 ctgatgtatg aagcttgttt aagatcagtg cctgtcgttc ttctttcagc ataaaatcta
    42721 ctcctgtgtg cctgcctttt cgcggagcag gatttcaatg taagcaagga tcagaggggc
    42781 tacgcctttt gcctcatttt ttacaatggg ttctctcata tagtagtcaa aggtgccttc
    42841 ccgcatttct ttatttccaa gtcctgcaac caggcagatg ccgcctaact gaagttctcc
    42901 gttttcttca gagagatatg tttcacagat gccggtaaat gccctttctc caaaggtaaa
    42961 atagctttca tccagatagc ccagacgcac gctcttcatg attgcatagg cgaagatggc
    43021 ggagccggag gtttccagat agtttggctt aatgcggcct ctgtttacaa cctgatacca
    43081 cataccggtt tccttgtcct gatagggcag catggcgtca atcagttcct tataaatagc
    43141 gcccagcttt tccttctcct gggccatgga tcccggcatt acctccatgg tatcgatcag
    43201 ggccatggca taccagccca gtgcgcgcag ccagaaattg tctgacaggc ctgtgacggg
    43261 gtcgcaccag aaggattctc tggaatcatc atatgcgtgg tagtacaggc cgttgcgtgt
    43321 gtctttcatg aggtcatata catgaaggaa ctgctcaaag gagtcccggc agttggcacc
    43381 attattatag gttacttcgt actgcatata gaagggctgg gccatataca ggccatccag
    43441 ccagatctgg tttggataga tcatcttgtg ccagaagttg cctgttgtgg ttcttggctg
    43501 ccctttcagc tggctgtata ctgtgtcgat ggctttgcgg tacttctccc gtccggtaag
    43561 ctcatagaga tcgaaaaggg ttttgccggc atttacattg tccagattat attcctttgg
    43621 atcataggaa gtgatggagc cgtcctcctg cacaaagtag tcgataaagg catcggcaaa
    43681 ttccaggtat tttccatttc gggtaatatg gtacaattca agaatggcct tgatcataca
    43741 gccgtcaatg tagttccagg tagaagctcc gccgtttttg atcttttcga tgttccagac
    43801 cggagcctga ggcgtactct tgtctaacag ctggtcaatg taatggtcca aaatcttcat
    43861 catgatataa ttctccttta tcctttaaaa atagtaacac atatataagg gtttttaaag
    43921 acagtatgaa agaaaatgat atgtaaacgt ttgcagacca aaaacgggta ataaatgtca
    43981 tgactgtctg gatctaattt cccttaattt ggtaaaaaat acttgcatta ccaccaaaaa
    44041 tatcattttc tgtcttgaaa ccatcttatc acaaaaaaat agagaagaaa actagacata
    44101 ttgcataaaa agaaagtgta aaaatataca caatacataa ttgacaactg atagggttaa
    44161 tgatattatg aaattaccat gagaaataag cacgtttgct taaaagcata caaatttgta
    44221 aacgcttacc tagcggatgt gcacaatttt cagaacaatg ttgcaaacct ttgcaaaatg
    44281 tgtatgcgaa ggggaaaaga aagggagaga gtaaaatgaa gagaaatttc aaaaaagcag
    44341 tatctctggc tctggcatct accatggcat tatctctggc agcctgcggc ggtacgtccg
    44401 catctacaga tacaactgca gcaggtaagg ctgagggcgg cgacagcgca gcgagcaatg
    44461 aggaagttac cttaaagttt tcatggtggg gcggtgatgc ccgtcatgag gcaacagaaa
    44521 aggcaatcgc cgctttcatg gaaaagtatc caaacatcaa ggttgaggct gagtatggcg
    44581 catggaccgg ctgggaagag aagcaggctc tggcaatcca gggcggcaat gcagctgacg
    44641 taatgcagat caactggaac tggatcacat cctatagcgg aaacggtaag aatttcgtaa
    44701 atctggacga gtacagcgat gtgatcgacc tgacacagtt tgaccaggag gttctggatc
    44761 agtgtaaggc agacggaaag ctgatggcag ttccgatttc tctgacaggc cgtctgttct
    44821 actggaataa gaccacattt gatgagatcg gatgcgagct tcctacagac gaggcatcct
    44881 tactggcagc cggagaggca tttaaggcat acggcgacga ttattatcct ctggcactgg
    44941 gcgagtacga ccgtgcgatc ttcctggtat actacttaga gagcaagtac ggcaaggcct
    45001 gggtagagga cggagctgtt cagtatacaa aggaagagat ccaggaaggt atggatttca
    45061 tcacaaagct ggaagagaat catgtaattc ctactctggc tacacttcag ggcgatatgg
    45121 cagactctct tgacaagaac gctaagtgga tcaacggtaa gtatgcaggt atccttgagt
    45181 gggattcttc tgtaagcaag ttccagcagg cgatcgcaga gagtacagat aagcctggac
    45241 aggagctggt aatcggcgac ttcatcaagt tcggcgatca caacggcgga tttaccaagg
    45301 tatccatggc atttgcagtt gcagcaaatt ctgagcatcc gaaggaagca gccatgctga
    45361 tcaacttcct gttaaatgag gatgaaggca tcgaaatctc cggtactgag agaggtatcc
    45421 cttgctccaa gaaggcaatt gagattctca atgagaaggg aatcggcgat gagatggtta
    45481 aggaagcaaa ctccaaggtg actgcataca gcaagttccc tctggatccc tacttcgagc
    45541 ataatgactt aaaggctaac cctgacggcg tatactacaa ggtattcggt aagctgtccg
    45601 caggcaacac gacatctgcc gaggctgccg aggaactgct taagggcatt gatgagtgct
    45661 acagcaacta ctaatccgca agaaataaac ggcagcgtcc ctttcagacg ctgcctccct
    45721 ttgaagggaa cagtgaatat gacagaaaaa gaaatcagag aagcagttaa aaacaaagaa
    45781 aattacagca gtattgttac ttattttaaa acacagaaaa ctctcagcga ggcagatctg
    45841 gccttgctga tcgacatgat agaggagatg tctccggaga tctatgagca ttacagagcg
    45901 ctccaggaca tgttccgtga aggcataaga aaacatctgg aggcttccaa agaggcttcc
    45961 ggcgagcagc tcgcatacgc cataaaaaag ggctgcgccc tgggcactct cctggcagaa
    46021 aagtatgcgg accgcgtata aaagtacgaa atattatgga ggtaaccgta tgaaaaacaa
    46081 gaaaggattc tgggcgctga atatcggcct tctgtacatc ctgccctggc tggttggttt
    46141 tatcctgttt aaggtatatc ccttcctctc atcccttttt tacagcttca ccaactacga
    46201 ccttttcaat ggcattacca agtacggcat gatgaattac gagaagatct ttacggatcc
    46261 cgatatcatc cgcgcctttg tggtaacgtt taaatacgcc atttttgacg tgcccttaaa
    46321 gctgatgttc gctctgttta ttgcctacat cttaaacttt aagttaaagg gcgtaaactt
    46381 cttcagaacc gcttactaca tcccttccat tctgggcggc tccatcgcga tcgctatcct
    46441 gtggagagct gtatttaaca cagacggtct gatcaacacc gttttaggcg cgttcggtat
    46501 cccggagatc aactggatgg ccggcgacat gagcgccctg atggtgatcg tacttctccg
    46561 tgtatggcag ttcggttccg ccatggttat cttcctggca gccttaaagg gtgtgtctga
    46621 ggatctttat gaggcggctt ctatcgacgg cgccggcaag tggacacagt tctttaagat
    46681 taccgtaccc ctgatcacac cggttatttt ctacaacctg gttacccagt tatgccaggc
    46741 attccaggag ttcaacggac cgttccttgt aaccaagggc ggaccaaacg gagcaaccac
    46801 actgatctcc atgctgatct acaacaacgc attcctccgc cacaagatgg gtatggcaag
    46861 cgcccaggcg tggatcctgt tcttgattac tatgactatg accgttgttg catttgtcag
    46921 ccagaagaag tgggtttact attcagatga ggatgggagg tagaatcaga tgacaagaga
    46981 gacaagaaga aaaatcagtg ctgtgatccg ctacaccctt ctgatcctgg tagggattat
    47041 catggtgtat cctctgatct ggatggtggg agcaaccttt aagaccaact ccgagatctt
    47101 caccagcgca tggtttatcc cgaagaatcc tgtattcgac ggctatgaca atgcatttaa
    47161 gagctacgga ggaaagatcg atctggttaa atctctgatc aatacctaca agatcgttat
    47221 tccgaaggtt atctttaccg ttgtatccgc aacgatcaca gcatatggct ttggacgttt
    47281 caactttaaa ggaaagaaca tcttttttgc actgctgatg tctaccctgt tccttcctca
    47341 ggttgtatta aacgcacctc agtacatcat gttcaacaac tggaagtggg taaataccta
    47401 cctgcctctg gttatcccgt ccttatttgc agctgatacc tactttgtat tcatgctgat
    47461 ccagttctta cgaggcgtac caaaagagct ggaggaggcg gctaagattg acggctgtaa
    47521 caccttaaaa acactgtggt atgtaatcgt gcctatgtta aagccatcac tggtatcctg
    47581 tgcgctgttc cagttcatgt ggaccaacaa tgacttccag ggcccgttga tctatatcgc
    47641 tgatatggat aaatatacca actccgtata tctgcgtatg tctatggacg gcgacgtagg
    47701 cttccagtgg aaccggattc tggcaatgtc cctgatttcc attattcctt ccctggtggt
    47761 atttttctgc gcccaggatt cctttattga cggtatcgca gccggcggtg taaagggtta
    47821 aatgttacat ggtacattaa aaaacggagg attctctttc gggggattcc tctatttttg
    47881 ttaaaggaga caaaagctat ggaattaaaa gtaatctaca agaacgcaag atgtgcggta
    47941 attgaaattg aagacggagg acgctaccat acagcctctt cctggactat ctacttaaat
    48001 cacaaagagg cggcaaaaac agacaaggcg gtcaccagcc tgtatcatct gactcccgat
    48061 acccagtacg aagtagaggc ggtcaatgag gccggagaga ggattatgtc ctactttgct
    48121 acagactatg agtttgttac cttaaatgtc cgcgactttg gcgcaaaggg agatggggag
    48181 agcgatgaca cgatttttat ccaggcagcg atcatggcct gcccgaaaga gagtcgtgtg
    48241 ctgatcacgg caggaaccta ccggatctcc tctctgttcc ttaaggatga tgtttccatt
    48301 gagctggatg agggagccgt attgtctgct tttacagacc gcagccgttt tcctgtgttc
    48361 aaagggatga tccagagtta taatgaaaag gatgagtaca atctgggtac ctgggaggga
    48421 aatcctcttc ctatgtttac agctattatt acaggagtga acgtaaaaaa tgtaacgatt
    48481 tacggacagg gaacgataga tggaaatgca aacagagaga actggtggaa ggatcccaag
    48541 atcatgaacg gcgcattccg ccccagaatg gtatttttag agcggtgtga acatgtagtt
    48601 ctccagggta tcacggtaca gaacagccca agctggaatc tccatcctta tttttccgac
    48661 aaccttcgtt tcctggacat gaaggtatta aatccaaagg attctcccaa cactgatggc
    48721 ctggatccgg agtcctgtaa aaatgtggaa attacaggtg tgtatttctc tctgggcgat
    48781 gactgtattg ctttaaaatc aggaaaaatc tatatgggat ctacctataa gcgtccctgt
    48841 gaaaatgtaa ccatccgcca gtgctgtatg cgcgacggcc acggctccat taccatcgga
    48901 agcgagatgg ccggaggcgt gaagaatctg accgtaagag actgtatgtt ccttcatacg
    48961 gaccggggac tgcggatcaa gaccagaaga ggacgtggaa aggatgcggt agttcagggt
    49021 attttatttg aaaatatcac aatggatcat gtaatgactc cctttgtgat caattgtttc
    49081 tatttctgcg atccggatgg ccattctgag tatgtccgca gcaaggaagc cggaccggtg
    49141 gatgaccgcc tgccggagat cgctaccctt acctttagaa atattgaggc cagaaattgc
    49201 catgtggctg ctgcctatat gtatggcctg ccggaaaaga agatcggacg ggtagagatg
    49261 gagcatgtgc gggtaactta tgcagatgaa cctatgtgcg gccagccggc catgatggag
    49321 ggctgcagta agaccacctg ccgccaggga atctatgcaa ataatatcga agagcttata
    49381 ttaaaggatg tggagataag cggccaggaa gggcctgcag ttgtcacaga aaatatcggg
    49441 aatatgtaaa acggagggga taaagtggca gtaacgatca aggatatcgc taaaaaggcg
    49501 ggtgtttcct attcaacggt ttccagggcc ttaaacggag tggggggata ttccgagaca
    49561 agagacagga tccgccatct ggcgtctgaa atgggatatg tacccaatca ggcagcgatc
    49621 cacttgaaaa aatcacgttc ctatgtcatc ggactgtatt tttccacgat cagcaaaatg
    49681 acttctccct atgtgcttca cgatgtactt acaggggttt acagtattac gggaagcaag
    49741 tataatgtag tggtaaaggg tattgatatg catgaaccgg gtaccttaaa tcccagctat
    49801 tttgacggga tcattgttct gtctcagaaa aatgaggata tggaatttat ggatgaggtt
    49861 ctgtcaaaga agattcccat gacggttatc tgccgttccg tggatatcga tgcgcccaat
    49921 gtgacaacag acgaggccag ggccatggaa cgggccatgg attacctttt ggataatggc
    49981 catcggaagc tggcagtcat cgagggaagg ccagagctgg attctacccg cctgcgccac
    50041 agaggctggc ggatctccat gcgaaagcac gggctggatc ccgatcaggt gcctatgatt
    50101 aatggaaact atcgttacgg aagcggctac agcgcagcaa aacagctcct ttcctatgag
    50161 cctacggcaa ttctctgttt taatgatgag atggcctttg gagcaaggac agcgatcgat
    50221 gaggcggggc tgtccgtgcc gggagacgtt tctctgatcg gctttgataa ctgggatttg
    50281 tccggttatt ccgatatgca tctgactacg gtggagagaa acatgggcga tatcgccaga
    50341 gagggtgcca gagtgcttct gcgccgtctg gacgatggaa ttgtggataa caggagaatt
    50401 tatctggata ataagcttat catacgggat acggtacgga acctgaagga agaatagtcc
    50461 gtgaaccgat tgcagatcaa acaaaaggag aaggcagaca tgaaaaaact ggaagaatat
    50521 gtaagaagca ttcccgattt cccggagccg ggaatcattt tcagggatgt aacctctatt
    50581 ttacaggatg cagacggact gcagctggca gtagacagcc tgcgcgccat ggtgaaggat
    50641 ctggattatg acgtagtcgt tgggccggag tccagaggct ttattttcgg cgtaccggtg
    50701 gcctatgcag agcataagag ctttgtgcct gtgcgcaaaa agggaaagct tccctgcgag
    50761 accatttctg cagagtatga actagaatac ggaactgcag agatcgagat ccataaggac
    50821 gccatccgtc cgggccagaa ggttgtgatc gtagacgatc tgatcgccac cggaggcact
    50881 acagaggcga tcataaagct gatcgaacag ctgggaggcc aggtggtaaa aatctgtttt
    50941 gtaatggaac tggccggatt aaagggcaga gagaagttaa aggattatgc agtggactct
    51001 gcaatcgttt atgagggaaa gtaattttcc gtccagaagc gttcattctg ttgaaaaatg
    51061 ggatggacgc ttttttgaac acatattttt cacattttcc cctgtgaaac tatcttgaca
    51121 ttaaattttt aagtctgtat aataccaaaa gttaggacgc taactatctg aaagcagtgc
    51181 atacgaagag gtgaaatgat gcaggatatg gaatgcagaa tggaaacaat gcatatggga
    51241 aggatgatcc atatgctctc tcatcagtta aagcgtagga acatccagga ggagctgatg
    51301 gatgagggat tgaccgggtc tcagcggcat attatgaagt ttatccttct gcggaccctt
    51361 catacggatg tgtatcagaa ggatatcgag gaagagttcc gtatccgcaa atcaacggta
    51421 acagggatcc tgaaactttt ggaaaaaaat ggattcatca ggagagaaag cgttcctcag
    51481 gatgcaaggc tcaagcgcat tgtgccgaca gccagggcag aggcactgcg tccccagatt
    51541 ttagagcagc tggggagaac ggaagccctt cttatggagg gaatcccgga agaagagctt
    51601 catatctggc gcagtgtgat gtgccggatg ttcggcaatc ttgtaaaagc ggaggaggaa
    51661 gcgtctaaaa caaataaaaa cataaaggag gaagaggaaa aggatgaata aattactttt
    51721 aaaatcggtc cgggaataca aaaagcagtc tatactgact cccatatttg tattgctgga
    51781 ggtgctgtta gaagttatga tccccatgga aatggcaaag atcattgaca ttggaatcgc
    51841 aggaggcaat ctgccttaca tcatccagag gggcttcata ctggttgtga tggcaatgct
    51901 tgccctgtat ttcggcgtaa cagccggaaa gacggcggca atcgcggcgg cgggatatgc
    51961 aaaaaatctg cgccatgata tattttacag ggttcaggag ttttcgttta agaatatcga
    52021 ccatttctct acctcaggtc ttgtaacccg tatgactacg gatattacca atgttcagat
    52081 ggcttatatg atgagcctgc gcctgttagc cagagcgccc atcatggtag ctttatcatg
    52141 ggttatgact ctgaccatca atccaaagat tgcgctttta tttttagttg tgatccccgt
    52201 tctgggcggc gccctgattt ttattgcgaa gaaggcccat ccccattttg taaaggtttt
    52261 tgacgagtac gatattttaa ataattctgt acaggaaaat gtaaacgctt ccagagtggt
    52321 aaaatctttt gtacgggaag attatgaaat cggtaagttc catgatatat ccaagtacgt
    52381 ttacaagctt tttacaaagg ctgaagggat tgtggcatgg aattctcctg taatgcagac
    52441 ggtaatttat atagtgatca tccttctggt agcaatcagc ggcaggagca ttgtctttgg
    52501 aaccatggag acaggagaga tgacaagcgt gatcgtctat gctatgcaga tcatgatgtc
    52561 cctgatgatg gttaccttcg tctttgttat gatcatgatt gctgaggcat ccacagaccg
    52621 tattaccagt gtccttacgg aagtgcctga aatgcaggca aaggagcagg cggtgaagga
    52681 ggttgcggac ggaagcatca cctttgagca tgtagatttc agctatgcag gtgaaggcgg
    52741 ccctctttcc ttaaaggatg taaatctttc catcaagccg ggacagaccg taggcattat
    52801 cggagggacc ggaagctcca aatccacgct agtacagctg attcccagat tatatgatgt
    52861 gacaaaggga agcgtaaagg taggcggaat tgatgtaaga gagtacgacc tggaggttct
    52921 gagagaccag gtttccatgg tgctccagaa aaatgtgctt ttcacaggaa gcatttatga
    52981 gaatatccgc tggggcaatg aaagggcctc caatgaggaa gtggagcggg tatgccgtct
    53041 ggctcaggcg gacgggtttg tgaaggagtt tcctcacggt tatgatacta tgatcgttca
    53101 gggcggaaac aacgtatccg gcggccagaa gcagcgcctg tgtattgcga gagcgctgtt
    53161 aaagcggcct aagatcctga tcctggatga ttctaccagt gcggtagaca caaagacgga
    53221 tgctctcatc cgcagggcct tcagagagga gattcccgat actacaaaga tcattattgc
    53281 ccagcgggtt tcctccattg aggacgcaga tcagatcatt gttctggagg aaggacagat
    53341 tgtgggcgtg ggtacttcca gtgagcttct gcgcaccaat gagatctacc gtgaagtata
    53401 tgagtctcag atgaagggag gcgaagagaa tgagcagtaa gaaactacag atttccaaaa
    53461 acacggctca gaccgcaaag cgtcttttaa aatatgtgac agaaacgtat aagctgcgct
    53521 tttttctggt atttatctgt atctttatca gttcaggagc aagcatttcc gtatctctgt
    53581 cgctgaaatt tctgctggac gactttatta tcccgctgat cggacagcag acgccggatt
    53641 ttgccgggct gtaccgggcg ctgggaacgc tggcctgtat tttcgttgcg ggcgtacttg
    53701 ccgcctttac ctatacaaga ctgatggtct atatcggaca gggtgtgtta aaacggatcc
    53761 gtgacgatat gtttgagcac atgcagaccc ttccgatccg gtattttgat cagaatacaa
    53821 ccggctctat catgagtctt tacaccaacg atacagacac gctgcgccag atgatcagcc
    53881 agtccatccc ccaggcgctg tcttcctttt ttacgatcgt agtaactttt atctccatgc
    53941 tggtgttaag ccctgctctt acggtgctgg ctgtactggt tattgttgtc atgcttttcg
    54001 tatccagaaa gatcggagga aacagcggaa aatattttgt gcgccagcag gtggctgtag
    54061 cggacataac aggctttgtg gaagagcgga tgaacggcca gagagtggtt aaggtattta
    54121 accatgagcg caaatccgag gaggagtttg acaaattaaa tgaagcgctt tttgaatgtg
    54181 cttctcaggc caataccttt gccaacatta tggggcctgt gatcggaaac attggaaata
    54241 tgcagtttgt gcttacggca gtgctgggag gtttcctgtc tgtgctggga gtcggcggca
    54301 ttaccctggg tgtgatggct tcctacctgc agtttacaaa gagctttacc cagcccttta
    54361 tgcaggtggc ccagcagttt aacgccatta tcatggctct ggcaggcgcg gaacgtattt
    54421 ttgccctgat tgatgagaag ccggaaaagg atgaaggcta tgtaactctg gtcaatgcca
    54481 gaaaggatgc ggacggggtg atttctgaat gtaaggagcg taccggcatg tgggcctgga
    54541 agcatcccca cggcgacggc agtgtgacct atacagagct tacaggcgac gttcggtttg
    54601 agaatgtgac ctttggttat gatgatgaaa aggtgatcct ccatgatatc agcctgtttg
    54661 caaagcccgg tcaaaagctg gcatttgtag gctctacagg agccggaaag accaccatta
    54721 ccaacctgat caaccgtttt tacgatattc aggatggaaa gatccgttat gacggcatca
    54781 atattacgaa gatcaggaag gacgatttaa ggcgttctct gggaatcgtg cttcaggata
    54841 cccatttatt tacagggaca atccgggaaa atatccgcta cggaaagctg gacgctacag
    54901 atgaagaggt atatgcggct gcaaagctgg cccatgcgga tcagtttatc cgtatgctgc
    54961 ctcagggcta tgataccatg ttaagcggag acggcgagga gctgtcccag ggccagagac
    55021 agctcttatc tatcgccagg gctgcggtgg cagatccgcc agtattgatc ttagacgagg
    55081 caacctccag tattgatacc agaaccgaga gtattgtgca gaaaggtatg gacaatctga
    55141 tgaaggggcg caccgtattt gtgatcgccc accgtctgtc caccatccgc aacagcaacg
    55201 cgatcattgt gctggatcat ggacggatca tcgagcgggg agaccatgag gatctgatcc
    55261 gcttaaaggg aacgtattat cagctctata cgggaaagct ggaattgtca taaaggcccg
    55321 tttggcgctg acagcatgaa ataaacatat ggattcaggg ataaccgtac atgaaaagga
    55381 tgtatggata tccctgaatt tttatttgtt tcggtcatgg acgccatatt ttcaaggacg
    55441 gccttgcttt ttcacagaaa taaaattata atgataacta attatttcta agaaaaaggt
    55501 gaccacacat atgggaaaag agggaacaaa agaaaaactg gatattgaac tggaagcgcc
    55561 ggctgatttt accagtccgg aggtactgta tcaggatctg atccgcgcca tccgaaagta
    55621 ccatccgtcg gatgatctta ccatgatcga aaaggcatat aaaatcgctg atgaggctca
    55681 caaggagcag aagcgcaaat caggagagcc ctatattatc catccgctct gcgtggcgat
    55741 cattctggct gatctggaaa tggataagga gaccattgca gcgggccttc tccatgatgt
    55801 ggttgaagac accattatga ctcttgagca gctgacaaag gagtttggct ctgaggtagc
    55861 ctttctggta gacggcgtta ccaagctgac ccagttaaac tgggacaagg ataaggtgga
    55921 gatccaggcg gagaacctgc ggaagatgtt tcttgccatg gcaaaggata tccgcgtcat
    55981 cattatcaag ctggcagacc gtctccacaa tatgcggact ggaaaatact ggaagccgga
    56041 gaagcagaag gaaaaggcca gagagacact ggagatctat gctcccattg cagaccgtct
    56101 gggtatttcc aagattaaga tcgagctgga tgacctgtcc ttaaaattcc tccatccgga
    56161 tgtgtattac gatctggtag aaaaggtgga tctgcgaaag gacgccagag aggcctttgt
    56221 ccagtccatt gtatctgagg taaaagagca tatggacgag gcgggcatcg aggcttccgt
    56281 aggcggcagg gtgaagcatt tcttcagcat atataaaaag atggtcaagc agaacaagac
    56341 cctggatcag atttacgatc tttttgccgt gcgcatcatg gtagaaacgg tgaaggactg
    56401 ctatgcggct cttggcgtca tccacgagat gtataagccg atacctggtc gttttaaaga
    56461 ctatatcgcc atgccgaagc caaatatgta ccagtctctc catactacgc tcattggcag
    56521 cacaggacag ccctttgaga tccagatccg tacctacgaa atgcaccgca cggcggagta
    56581 cggtattgcg gctcactgga aatataagga aagcggcagc ggacaggtag cagcaggcaa
    56641 ggaagaagaa aagatgagct ggctgcgcca gatcctggag tggcagaagg atacggatga
    56701 ttccaaagaa ttcttaaata tggtaaaggg cgatctggat ctgttttccg acagtgtata
    56761 ctgctttacg ccctcgggtg atgtaaagac ccttccaagc ggctctacgc ctattgactt
    56821 tgcctactgc atccacagtg cggttggcaa caagatggta ggagcaaggg taaacggaaa
    56881 gctggttccc attgagtatg tgatccagaa cggcgaccag atcgagattg tcacctccca
    56941 gaactgcaag ggtcccagcc gcgactggct gaaggtggta aagagcaccc aggccaaaaa
    57001 caagattaac cagtggttta agacccagat gaaggaggac aatatcatcc ggggccgtga
    57061 ggcggtagag cgctactgca aggccaaggg catcaactgg gcggagcttg gaaaaccgga
    57121 gtttatggaa aaggttatgc accggtatgc ctttaaggac tgggattccg tgctggcatc
    57181 catcggccac ggcggcctga aggaaggcca ggtcatcaat aagatgatgg aggagcggga
    57241 gaagaagcgc aagcgtgaga tcacagatgc agacgtattg ggcggcattg ccgatacaga
    57301 cggccgctcc ggagaagcga cggtgcgcaa aaataagagc ggcattgtgg tgaagggact
    57361 tcatgacctg gcagtgcgtt tttcacgctg ctgcaatccg gttcccggcg atgagatcgt
    57421 aggctttgtc accagaggcc gcggtgtgtc gatccaccgt acagattgta ttaatgtgat
    57481 caatcttcct caggaagaga gagctcgtct gatcgatgca gaatggcagg tggcggaaac
    57541 agcgggaagt acggagcagt actctacgga aattcagatt ttcgccaata accgcattgg
    57601 catgtttgcg gatatttcca aggtatttac agagaagcag attgatatta cttctatgaa
    57661 tgtgagaacc agcaagcagg gcaaggcaac cattatcatg acctttgaca ttcacggcac
    57721 ggaagaactg aaccggatca cagataagat ccgccagatt gaaggcgtgc tggatatcca
    57781 gagaaccacg ggctaaagga ggatgcgtat gagcgatttc agaatcaaaa cctgcgtatt
    57841 ggggccggta agcacgaact gttacctggt ttacaacgat acaaccaaag aggctgtggc
    57901 agtagacccc ggagacaacg gagattatat tttaaataaa tgcagtgagc ttgggataaa
    57961 gcttcaggct gtgcttctga cccatgggca ttttgaccat atcggagcgg ctcttcagat
    58021 agcaagggcc tttaccattc ccatctatgc atcggaaacc gaggatggta tgctggcgga
    58081 tacgtctttg aatctgaccg ctcattttac aggccattct ctgagttttc atgcagacag
    58141 gctggtacat gacggggatg agctggagct tttgggatat aagtggaaag tcattgcaac
    58201 tcccggccat accgcaggct ctgtgtgcta ttatctgccg gaagaagagg tgcttctgtc
    58261 tggagatact ctgttccagg attcctatgg acggacagat ctgccgacag gaagcagcag
    58321 agcgctggtg gattctgtac ttaggaagct gtttatactg ccgggagata ctatggtcta
    58381 tccgggccat ggagagccca ctaccattgg ttatgaggcg cagaacaatc ctattgcatg
    58441 ctatagatag aaaatagaaa gcaggaacaa aatgatcggt ttactgatac aggataatgc
    58501 atacgaacag gacatcagag aacttctgat gtctttttat ccgggcgaaa catatgccca
    58561 tgaaataaaa gacggagtgg atttttatgt gcagacccgt ctcatacaga aggaagagga
    58621 cggctgcgct gcctcggagg aggatgcggc cggacaggaa tggaatgtag ggattgccct
    58681 ctgggaacag ataaaagagg gagaggccgc aggctgcagt ccttattttc cagtgccgga
    58741 aggactggag gactttcacc ttgcgtcatc ctccagagga tattcggatc tttccgacca
    58801 cagcgagacg aaaaatgtgg taaagcagct attttaccgt atgctcacag aacgcaccgg
    58861 ccatgagctg ccctggggat ccctcacggg aatccggccc accaagattg ctttaagccg
    58921 tctggaggag ggctggaagg aggaagagat ccgctccttt atgaaaaaaa actatctggc
    58981 gagcgatgaa aagattgatt tgagcattga aattgccgca agggagaaaa tgcttttaga
    59041 gccgttggat tatgacaggg gatacagcct gtatgtggga atcccattct gtcccaccac
    59101 ctgcctttat tgttccttta cctcctatcc catcagcaaa tggaaggggc gcaccggcct
    59161 ttatctggaa gcattattta aggaaatgga atatgtggca gacaagatga agggacgccc
    59221 tctggatacc atctactttg gaggcggaac cccaactgct ctgccggcag aggacattga
    59281 cgcgatcctg acaaagctgg atcagctgtt tgatacggag aatgctttgg aatttacggt
    59341 ggaggcaggg cggcctgaca gtatcacaga ggaaaaactt cgggtgctga gagaccatgg
    59401 catcaccagg atttccataa atccccagac tatgaaccag aagactctgg atctgatcgg
    59461 ccgccgccat acggtggaga tggtaaagga aaagttcctc atggcaagag agctgggctt
    59521 tgacaatatc aatatggatc tgatcatggg gcttccggag gaggatttag acgatgtacg
    59581 ccacacactg gaagagatcg aggctctcag gcccgattct cttacggttc attccctggc
    59641 catcaagcgg gctgcccgtt taaatatgtt taaggaagag tacggcggct taaagataaa
    59701 caatacacct gagatgatcg ccatgagcga ggcctgcgcc cgtcgccttg gcatggaacc
    59761 ctattatctt taccgccaga aaaacatggc cggaaatttt gaaaacgtag gctattccct
    59821 tcccggcaaa gcctgcattt acaatatcct catcatggag gagcttcaga ccatcgccgc
    59881 ctgcggcgca ggcaccacca caaaggttgt attccctgca gaaaaccgca gggaacggtg
    59941 cgagaatgtg aaggaagtgg agcagtatat cagcaggatt gatgagatga tcgggcggaa
    60001 ggagaggata tgggggtaaa cggtatattc cagccctgat ttcccgcgaa tttcctcaaa
    60061 acacttgcaa aatagaaaaa gtacactaca atagtaatca cgaaacaaaa cgcaaattat
    60121 ggagaaaata ctatgccgtt aacgaaaaaa ccagtaaccg gaatgaagga tatcacgcct
    60181 gccgagatgc agatccgtga gtatgttatg aaccagatcc gtgaaaccta taagagcttt
    60241 ggcttttccc agatcgaaac tccctgtgta gagcacattg agaacctgac cagcaagcag
    60301 ggcggagaca atgaaaaact gattttcaag gtattaaaaa gaggcgacaa gttaaatctg
    60361 gaaaccgcac aggatgaaaa tgatttaacc gacagcggtc tgcgctacga cctgacactg
    60421 ccgctgtccc gctattatgc aaacaatgca tccagccttc caagcccctt taaggcgcta
    60481 cagatgggaa gcgtatggag ggcagaccgt ccccagaagg gccgtttccg tcagtttacc
    60541 cagtgtgata ttgatattct gggtgatgct accaatctgg cagagattga gctgatttta
    60601 gccactacaa ccgctcttgg aaaaatctgc cctgacaatg gttttaccgt ccgtatcaac
    60661 gaccgtggta tcctttttgg catggcccgc tactgcggct tctctgagga ggctatggac
    60721 agtgttctga ttacccttga caagatggac aagatcggtt ttgagggtgt ggagaaggag
    60781 cttcttgaaa atggaaatgc aaaggaaagc gtagaccgtt atctggagct tctccgtacc
    60841 gttacaagag atgcagaggg tgtaaaggct ctgggcgaga ccttaaagga tgtgatggac
    60901 ccggctgtat gtcagggact ggtacacatt atggagaccg ttaaggatgt ggctgaaact
    60961 gagtttggac ttgtatttga tcccactctg gttcgtggta tgtcctacta cacaggcact
    61021 attttcgagg tatccatgga gggcttcggc ggttctgttg ccggcggcgg acgctacgat
    61081 aagatgatcg gaaagttcac tggcatggaa acgccggcct gcggcttttc catcggtttt
    61141 gagcgcattg tcaccatcct cttagataac ggctttaccg ttccgggagc cggagaaaag
    61201 aaggcatatc tctttgaaaa gggcatcgat tccgcagctc tggcagcagt gattcgtgag
    61261 gccatggagg agagaaagaa cggcgttcag gtgctggtag cccagatgaa taaaaacaag
    61321 aagttccaga aggagcagct tggcaaagag ggctatggag agtttaagga gttttacaga
    61381 gagagcctga agcattaagg gcgaaaatgc atttgtgcat tttgcagcca tcttctgaac
    61441 agaacattca ggttccggtt tacgactgta agaaaggcta agaggctgaa acatttctaa
    61501 atacaagagc catatcaaaa actcgctgcg ctcaaacagg tttgatatgg ctcttaacga
    61561 aacgtttaac cctctaaggc tttctaacag cctccaaagt cacctgaatg ttcatagttc
    61621 aggagcttac tgcaaaatgc gcgaatgcga taaatcttta cgatgataaa ggagaataat
    61681 catggcagaa tcaatggctg ggttgaagcg tacccacaga tgtacagagg taactgcagc
    61741 gcagatcggt gaaaatgtca ccgttatggg ctgggttcag aagagcagaa ataaaggcgg
    61801 cattattttc gtagacttaa gagaccgttc cggtatttta cagctgattt tcgaggaaaa
    61861 tgactgcggc gctgagaact tcgcaaaggc tgagaagtta agaagcgagt ttgtagtggc
    61921 agtagaaggc cgcgtagagg cccgttccgg cgctgtaaat gaaaatctgg ctacaggagc
    61981 catcgagatc cgtgccaatt ccctgcggat cctggctgag tctgagacac cgccattccc
    62041 tattgaggag aagagcaaga caaaggaaga gctgcgtctg gagtaccgtt tccttgattt
    62101 gagacggcct gatatccagc acaatctgat tacccgcagc cgcgtggcaa ctctcacaag
    62161 agcatttctg gcagaagagg gcttccttga gattgagact cctactctga ttaagagcac
    62221 ccctgaaggc gcaagagatt acctggttcc cagccgtgtg catcccggtt ctttctatgc
    62281 tctgccccag tctccccagc tgtttaagca gctgttaatg gtatccggct atgaccgcta
    62341 tttccagctg gcccgctgct accgcgacga ggatctgcgt gcagaccgcc agccggaatt
    62401 tacacagatc gatatggagc tgtcctttgt ggatgtagat gatgttctgg atgtaaatga
    62461 gcgtctctta cagaagctct ttaaggaaat ctgcggttat gagctgcagc ttcccatcca
    62521 gcgcatgaca tggagagagg cgatgaaccg cttcggttcc gacaagccgg atctgcgctt
    62581 tggaatggaa ttaaagaatg tttccgaggt tgtgaaggac tgcgaatttg tggtatttaa
    62641 gggcgctctg gaaaatggcg gttctgtccg cggtatcaat gctgagggcc agggccatat
    62701 gcctagaaag aaaatcgacg ctctggtaga gtatgcaaag ggctttgggg caaagggtct
    62761 tgcatacatt gccatcaatg aggacggcac ctttaagtct tcctttgcaa agtttatgaa
    62821 ggaagaggaa atggttgccc tgatcgctgc catggacgga aaagcgggcg atctccttct
    62881 ctttgcagca gataaggaca aggttgtatt cgacgtgttg ggcaacctgc gtctggagct
    62941 ggcaagacag ctggatctgc tgaaaaaaga tgactttaag ttcctgtggg taactgagtt
    63001 cccgcttctt gagtattctg aggaagagga ccgctatgtg gcaatgcatc atccatttac
    63061 catgccgatg gaggaggatc tgcagtacct tgactctgat ccaggccgtg tccgtgcaaa
    63121 ggcatacgat atcgtattaa acggcgtgga aatgggcggc ggttctgtcc gtatccatca
    63181 ggccgatatc cagtcaaaga tgtttgaggt tctgggcttt actcctgagc gtgcacagga
    63241 acagttcggc ttcctgttaa aggcattcaa gtacggtgtt ccgcctcatg caggtctggc
    63301 atacggcctt gaccgtatgg ttatgctgat ggtaggcgca gacagcatcc gtgatgtgat
    63361 tgccttccct aaggtgaagg acgcatcctg tctgatgacg gaagctcctg ctcctgtaga
    63421 tggaaaacag ctggaagagc tgggaatcca ggttgcggaa gaagaaaaac agtaatattt
    63481 taaaatttga ggcagtgcct tttgagaaat gagatctcag ggcactgctt ttttattttt
    63541 taaatttaac tattgacatt cagaaaaaaa gaactatact gtttatataa acatatgaac
    63601 atttattcat atattaagcg gccgaaggag aggaggaaaa aaatggcatt aaatgatgta
    63661 gagtgctgtg atttttatca ggttcatgag gatgtggtaa agtccgtgac tgataagatg
    63721 ccggatgagg atcagctgta cgatttggcg gagattttta aggtgtttgg cgattccaca
    63781 cggattaaga ttttgtatgt attgtttgaa tcagagatgt gcgtgtgcga tattgcccag
    63841 cttttaaaca tgaatcagtc cgctatttcc catcagctta ggattttaaa gcagaaccgt
    63901 ctggtgaaga accggcggga aggaaaggcc gtattttatt ctctggcaga cagccatgta
    63961 aagaccatac tcagtcaggg tatggatcat atatcagagt aagaatataa aaatggagga
    64021 aaataagaac atgaaaaaga aattcaaatt aactaattta gactgtgcga actgcgctgc
    64081 aaagatggaa aacgccatta aaaagattga cggggtaagg gatgccaatg tgagctttat
    64141 gactcagaag atgacgattg aagcggatga cagccgtttt gatgagatta tggaggaagt
    64201 gcagtctgta tgcagaaaag tagagccgga ctgtacaatt cagatgtaga gccgttgaga
    64261 atgatgcatc aggaggaata acggaacatg acagtaaagc agaagcgtat gcttatacgt
    64321 atccttggaa cctttggaat ctatattgcc ctcacagtaa cagagcattt gggacttctg
    64381 ccgtttgggg agaaaagccc ggtatggccg gcgctgtatc tgatccccta ttttctcatt
    64441 ggatgggata ttatttaccg ggctctgcgc aatatcagaa acggacaggt gtttgatgaa
    64501 aactttctga tggctctggc tacagtagga gcatttgcgg tacaacagta ctcagaagca
    64561 gttgcggtga tgctgtttta tcaggtaggg gagctgtttc agaattatgc cgtaaaccgt
    64621 tcccgccagt caatctcaga cctgatggat atttgcccgg agtatgccaa tattgaagaa
    64681 gacggacagc taaagcaggt ggatccggat gatgtatcag tgggggatat aattgtcata
    64741 aaggccggag agaggatccc tctggacgga agggttattt ccggagactc catgatcgat
    64801 acatctgctc ttacaggaga gtccgtcccg agaagagtca gagcaggaga tgagatgatc
    64861 agtggatgcg tcaatggaag cggactgctg cgggtagagg ttacgaaaga atttgacgat
    64921 tccacagtag cacgtattct ggaactggta gagaatgcca gcagcaaaaa ggcgccggtg
    64981 gagaacttta ttacccggtt tgcaaggtat tatactccgg ttgtggtgat tgcggcagta
    65041 gttcttgcta ttcttcctcc gctgtttttt ggacagacat ggagcgaagg gatacgcaga
    65101 gcctgtactt tcctggtgat ttcctgtccc tgtgcactgg tcatttccgt tcccatgagc
    65161 ttcttttccg gaatcggagc ggcttctaaa aaaggtgtat tggtaaaggg aagtaattat
    65221 ctggaggctc ttgcaaatgt ccatacagtg gtatttgaca agacgggaac ccttactaag
    65281 ggagaattta aggtgacagg catttatccg tccggagatg cggctcagga aacaatagag
    65341 aaaaggcagg atacagataa agctgacggc tggtcggctt cccagttggc tctgttagaa
    65401 ctggcagcct tggcagaaag ctcctctgac catcctgttt ccaggtctat ccgtgaagct
    65461 tgggggcatt cttttgagca tacgcggatc gcagatgcgg aggagattcc cggtcatgga
    65521 atccgtgtga aggcagatgg acgcactgtg ctggcaggaa atgaccgtct gatgaagaag
    65581 gaaaatattg cctataagcc atgtccgggc gtgggaacgg tggtttatct agcagaggaa
    65641 ggaaattttc tgggttccat tcttatttcc gatgtaataa aagaaggcat tccagaagca
    65701 atacgcgatc ttaaaaaggc cggtgctgca aagacagtga tgcttacagg cgaccggagt
    65761 aaaaccgggg aagcggtagc aaaggaaatc ggcctggatg aggttcatgc agagctcctc
    65821 cccggcgaca aggtagatat ggtagaaaaa ttgctggcag aatgcaaggg aaaggaaaag
    65881 ctggtctttg taggcgacgg catcaatgac gccccggtgc tctctcgtgc agatgtggga
    65941 atcgctatgg gaagcatggg atcggacgca gctatcgagg cggctgatat tgtccttatg
    66001 gatgatgatc ccagaaagat tgcagccgtt gtccgtattt ctcgtaaaac actccgaatt
    66061 gtgatgcaga atatcgtctt tgccctgggc gtgaaattga tcgttctggc gatgggagcc
    66121 tttggcgcgg ctaatatgtg ggaagctgtg tttgcggatg tgggagtgtc cgtgatcgcg
    66181 attttgaatg ccatgagggc tctgaaggga taattatata gacagattcc tttgtttatg
    66241 aagctgattg gtagataaaa acgatataat ggaatgaaaa aaggacgcat ctgctcaggc
    66301 agaacggcgt cctttttagg tgtcatatga aaatgtcaaa agcagaattt atctctgtaa
    66361 aacggaagaa atcaaaccag cacatcctcc agacttctct tgggaacata atgcacctga
    66421 ttttcatccc tgtagtacgt tgtgcttcca tcatccccta caggtacaag ccggacttcc
    66481 tctttgggtt ttccaagggc cagaaccagg cccacagaat agttttccgg attaagcttt
    66541 aaaatggaca gaatctcact gcgcctgcag cttccgatca tgcagccgcc atagcccttt
    66601 tctgcagctg acagcatgag cgtctgggcg gcgattccct catcccactg tttatttttg
    66661 gccacagtca gatcacagag aatgacgata taggcagagg gacgttctcc cttttccggt
    66721 ccgtcccagt ccttcagggc gcctgcccac aaaaggcagg ggaaaattgc cgcgcattcc
    66781 tccggagtac ggatgatgcg gtattttagg ggctgacagt tggctgtgca gcctgtaagg
    66841 cgcaccgtat cgaccatatc tgtcagctct tcctgcggaa tggatatatc ttcatagaac
    66901 cgccgatagg agcggcagct ttttacaagg tctttaagca tgaatagatt cctccttaag
    66961 ctcctttgga agctcatttc cctgggtaat atgtttctta attgcttcaa agctttccgg
    67021 actgattaca tgctccatac ggcaggcatc ctctgcagct gtctgaggat ccacacccag
    67081 atagatcagc cagttggaca aaagaatatg gcgttcgtac ataacgtcag caatggactg
    67141 tccagtctca gtaagtgtga tgtgaccctg ttcatccatg cggatatagc cgttttccct
    67201 caggtttttc atagctacgc tgacactggg ctttgaaaaa ctcagttcat ttgcaatatc
    67261 aatggagcgg acatagggtt tatggcggct cagcatataa atcgtttcca ggtagttttc
    67321 agcggattcc tgtattttca aaacaaacat tcccccttac ggttttcttt cccgtctgcc
    67381 tgaaaaacgg gaactgctac ctcttcagtg tatcatagaa gaaaaggatt gacaagcaaa
    67441 ccatgattca ttttgtccac tgatgatatg cccggctctt tcacagacag ctgtttagtt
    67501 tgcctttacc tgcttccaca tctcattgta catatcattt acttcctcgc ccagatactg
    67561 aacgctctcg ctgtttttca gctgggaaag atccgggaac acagccttgt tattctgcag
    67621 ctcctcgtcc agtagctcaa aggcgccctt gttaggggta gcgtaggtaa tgtattcaaa
    67681 gttctttttt gcaatgtcag ggcggcacag gaagttaatc catttctcgg cattttcctt
    67741 gtgctctgca ttggcaggga tgacccagca gtcgatccag taggaggttc cctcttcggg
    67801 aagcacatac tccagatcaa attcagaacc ctgttccttt gcggtatcct gaatataaag
    67861 gagatcaccg gagtagagca tggcagcagc tgcctcgccg ttaatcatct tgtcccttac
    67921 ctggtcgatt acataggcct gaaccagggg cttctgctca atgagcatat ctctagcctc
    67981 agccagctga tccgggtctg tggtattcaa agagtagccc ttgatcttta atgcggccat
    68041 aaatgcatag cgcagggtat tgtgcatcag gatctcgccc tctaactcag gattccacag
    68101 atctgcccat ttggtgggat atgggatgcc aagctcatca aagcgctgct tattgtagag
    68161 gattccttct gtgccccagc agtagggaac ggcatatttg ttttccggat caaaatcctt
    68221 tgccatatcc atatattccg ggccgatttc tttgatattg ggtacattgt caaaattgat
    68281 ttcagccagc aggttattct ggatcatacg ctggatggta tagtcggaag gacagatcac
    68341 atcgtacttt accgctcccg cttctatgat gggatacatt tcttcatttg tttcaaaaag
    68401 gtcgtatacg acttcgattc ctgtttcttc ctcaaattcg tcaattacgc tttcgtcaat
    68461 gtattcgccc cagttgtata catataattt gttgtcttcg ccggagcctt cctcagcccc
    68521 ggttttttgg ccggaaccgc aggcagtgag aagaccggcg gcaagggcca gagagagcat
    68581 gcccagtgaa acagctgttt tctttttcat tcttatcctc cattagtaaa tgcgtagatg
    68641 ggacagccgc agcagtccgt cagtttgcct tgacctcttt ccagagctcg ttataaaggg
    68701 cgtcggcttc ttcgccgggg tactgtatga tttcacagtt ggtgagagcg gagagatccg
    68761 ggaacagagc tttattctgt ttcagctcct catccagcag ctcataggca cctttgttgg
    68821 gagtggcata ggtaatgtac tcaaaatttt tctttgcgat atcggggcgg cacagaaagt
    68881 caatccattt ttctgcattt tctttgtgct ctgcattggc gggaatgacc caggagtcaa
    68941 cccagtagtt ggttccgtcc tccggaatta catactccag agtaaaatca gcttcctgct
    69001 ccttaactgc attctggata tagagcattt ctccggagta gatggctccc acggctgctt
    69061 ctccgccgat catcttatct cttacctgat caattacata agcctgaacc agaggcttct
    69121 ggcggatcag ctcatccttt gcctgctgca gttcatcagg attttgggta tttaaggaat
    69181 atccaaggct ctttaaagct acggtaaagg cgtcgcggac gctgttctgc atgagaattt
    69241 ctccctccag acgttcatcc cacagatcgg cccatttggt gggagcagga acgcccagct
    69301 cctcgatcat ctgcgtattg tagaggattc ccatggtacc ccagcagtag ggaacggaat
    69361 atttgttttc cgggtcaaaa cctttggaga tatccagata ttcctgtccg atttctttgt
    69421 aattggggat attgtcaaag ttgatttcag ccagaaggtt gttttcgatc atcttctgga
    69481 tcatatagtc ggagggacat accacatcgt agcttacgcc tccggcttca attacaggat
    69541 acatttcttc gttggtttca aatacgtcgt agaccacttg aataccggtt tccttttcaa
    69601 agtcgtcgat gacgctttcg tcaatatatt cgccccagtt gtatacataa agcttgttgt
    69661 catctcccga agaaccattt ttgccggaac cgccgcagcc ggtcagaaga cctgcggtca
    69721 tcagggcagc cagtgctcct gctgtgagga ggatgggtgt tcgtttcatg ggtcgctctc
    69781 ctttttaata gtaatgaatt atttcagtcc tttttcttaa aggaacggaa atttacgatc
    69841 agcagaagga tcagtacagt caggaaaatg actgtggaaa gggcatacat ggatggctta
    69901 atgcccctgc gtacctcgct gtaaatcagt gtagacaaag tattgatgcc tgcgcccttt
    69961 gtaaaatggg tgataataaa atcatccaga gacatggtaa aggataaaag gaagccggac
    70021 agcactccgg gcagaatatc cgggaagacc actttaaaaa atgcctgaag gggggtggct
    70081 cccagatcca gagctgcctc ataggtactc ttgtccgtct gcttcagctt gggcatgacg
    70141 cttaaaatca cataaggtat attaaaggtg atatgagcga tcagcacggt tttaaagcct
    70201 aatgatacgc caaaggcaat aaaggacagc atcagtgaaa tgcctgtaac gatatctgca
    70261 ttcagcatgg gaatgtttgt gatccccata ataattgtcc tgggaaccag cctcatggac
    70321 tgaatggcaa tagctgccgc tgttccgata acagctgccg caagggctga aacaaaggca
    70381 ataatcaggg tggttgacag ggcgctcata atggagcggc tgtcaaacat ctgcagatac
    70441 cagtcaagag taaaaccgcc ccaccgtgct ctggatttgg actggttaaa ggacaatacc
    70501 atcatagtga ggatgggtgc gtacaaaaag atcaggataa gcaccatata gaaatcggaa
    70561 atgatcttcc gtatcatttt tctgcttttt accatcagaa tgcgctccct tctccatttt
    70621 tgtcatattt ggcaatcata gccatgctga tcagaataaa gatcatcagc accagggaaa
    70681 gaccgcttcc ctggttccag ttgcttccgc gtgtgaactc ctgctcgatt acatttccga
    70741 tgagaaggat cttgctgccg cctaagaggt ctgagataac gaatgtggtc agagaaggca
    70801 caaataccat ggtgattccg ctgatgactc ccggaaggct taaaggaagc cagatcttta
    70861 taaaggtctg gaaggtattg gctcccagat cctgagccgc attgataacg ctgtcgtcaa
    70921 tcttggcaag tacattataa atcggaagaa ccataaaggg caggaagttg tataccatac
    70981 ccagtacaat agctgccgga gtattgataa tggagatacc gggcagatgg aaaaaggaaa
    71041 ggattccgtt gattacgccg tttttctcca gaagcgtctg ccatgcaagg gttctcagca
    71101 gaaaattcat ccacatgggg aggataaaaa taaatacgat aaagctgccg gccttatttc
    71161 cccgtcctct taaaatcata gccagaggat atgcaagaaa aaggcacagc agagtgctga
    71221 tcagggataa acccagagac agcaccagag ccttcatatg atccggttct gcgattgaca
    71281 ggatattttc caatgtaaaa gctccgtcac gtcccgtcag tccataccag agaatcatga
    71341 gcagaggaat aatagtaaaa ccggtcatcc agaccagata ggggccggag agaagggatt
    71401 tccccagatc gagatatttc ttttccttat tcatcgactc ccagcgcctc ctcatcctca
    71461 gacgcaggct tgttcatgat ctggatatca aagggatcta catggattcc cacctgcttt
    71521 ccaacgggga acatatcagt ggagtgaacc agccactcaa agccgtccgg ggtagtaact
    71581 tccatctcat agtgaacacc cttgaagatc agatgggtca ctgtgccgac aagcgtgccc
    71641 ttttccggtt ctacaaggtc gatatcttca ggacggatca cgacatccac tggcttattt
    71701 gtgccaaagc ccttatccac acaggcaaat tttgctccga agatctcaac cagctcgtcc
    71761 tgaagcatga tgccgtgaac gatgttgctt tcgccgataa agtcggctac aaaggcattt
    71821 tccggctcat tgtagatctg ctccggagat cccatctgct ggatatagcc ctggttcata
    71881 acaacaatgg tgtctgacat ggtaagagcc tcttcctgat cgtgggttac atagataaaa
    71941 gtgatgccaa gctcattttt caggcggatc agttcatact gcatatcttg gcgcagcttt
    72001 aaatccagag cgcccagagg ctcgtcaagg agcagcactc tcggttcgtt tacaatggct
    72061 cttgcaatgg caatacgctg ctgctgaccg ccggagagag aggttacatc gcgcttttcg
    72121 tagccggaga gattgaccag ctttaatgcg tagcggattt tgtcatcaat atagcttttg
    72181 ggcttgtttt ttattttcag gccaaaggca atattgtccg caatgctcat atgaggaaac
    72241 agggcatatt tctgaaaaac cgtatttaac tgccgcttgt tgggcggaag ctttgtgata
    72301 tcctgaccat caaagagaac ctttccggta tcgggactgg taaagcctcc caggatacgc
    72361 agagtagtgg tctttccgca gccgctgggg ccaagtaagg tgacgaagct gttttccctg
    72421 atggagagat tcagatcatc caggatcagc tcgccgtcaa agctttttga gatgtgctgt
    72481 aaatcaatca atggctggct catttgtctt ttctccttta taaagtgaag tgggtcataa
    72541 cattgtcttt tttacaacgg aggccgtatt aaaaactggg aggtgaactt acccagatga
    72601 gcatggcgcc gtttttgctt gaaagatagt gttttttatc tgctgtgtaa tagaaggatt
    72661 ctcccttttt ggcatggtat gtcttttttc ccagatggat ggaaacagaa cccttcagaa
    72721 tatagccaaa ttcttctccc tcatgaggtg tatccgggta ggtagaaccg ccggctttga
    72781 gcgttaagag aatgggttcc atggtgtttt tctgagcatt gggaatgatc catttgattt
    72841 cattgttgta ttcaggatct actttttcaa agtagtcctg agtgccgaat acgatctgtt
    72901 cctcaggctc ttccgtaaaa aactccccga tggtagttcc cagacactgg agaatatcca
    72961 tcagagtagc gatagagggg gaggtcagat tgcgttccag ctgagagata aaccccttgg
    73021 aaagctccga gcggtcggcc agctcctcct gagtcagtcc ctttaagaca cgaagctcct
    73081 tcagtctgtt tccgatatcc atacaattcc tccctgcacg gctgcaaaat atacaaaaag
    73141 tttagcaata ctaacccaat atataaataa tcaaaaagtt tagtaataat aaacaaggcg
    73201 aatttcatta tacatgaaat aaatcaggtg tcaatacctt ttctgaaagt tagcggttaa
    73261 caaataataa aaattatgat atgataggtt ttaacaaaat cggacttatt ttcaggaggt
    73321 aaatacaggt atgagcggca gatatatggc atatgtggga tcctactcct ataccgggaa
    73381 agcaaaggga atcacagtat ttgacgtaga tgtggagaag ggaacgttta aggagcgatg
    73441 cgagatggag gtaaataatt cttcctatgt aattgcctcc agcgatggaa agacattgta
    73501 ttccattgcc gatgaaggag tggtgtcttt ccggatcctg cagaacggcg ccctggcgcg
    73561 tttaaacagc gcgcccatcc gcggaatgag gggctgtcat ctgtgcagcg atctggagga
    73621 caagtttatc ttcgtatccg gttatcatga cggaaagacc acgatcctga gcttgaacaa
    73681 agacggctct gtagggggga tcgtggacgg tgtatatgat aaaggatacg gaagcgtggc
    73741 agagcgtaat ttccgccctc acgtgacatg cacccgccgg atgccggaca ataaatacgt
    73801 cctcagtgtg gataacggag tggatcaggt aaagatttac cgctttaatg ataaggaaca
    73861 gaagatggtt caggtagacg ccatccgctg cgacctggaa tctgcgccca gacatttccg
    73921 gttcagcagg gacggacgct tcctttatct gatgtacgag cttaaaaatg taattgatgt
    73981 atttacttat gagacagggg acagagctcc cgtaatcgag aagatccaga cgatttctac
    74041 tacctcgacg aaaaatccgg gcaatcttac ggcagcctgc gcgatgcgta tgtcctggga
    74101 tgagaagtat gtttactgca ccaatgcggg agagaatacg gtaagtatct acagcaggga
    74161 tgaggaaaca ggacttcttt ccatgatctg ctgcctgccg atcagcggcg aatatcctaa
    74221 ggacgtggct gttttcccgg atgggaagca tattgcgtcc attaaccatg acagcggcag
    74281 catttccttc tttaaggtag actatgagaa gggacttctg gtcatgagcg gaagaagcat
    74341 tccggttaat gaaccaaact gctgtgcgat tgtaaagatt ggagattagt catggcgggt
    74401 tcaatttacg ggacgctttt tcgcgtcagc acctggggag agtcccatgg aaagggcatt
    74461 ggcgtggtca tagacggctg tccggcaggt ctgcccttag atgaagaaga tattcagaga
    74521 tatttagacc ggagaaagcc gggtcagagc cgctttacca cagccagaca ggagggcgat
    74581 caggtggaaa tcctttctgg cgtctttgaa ggaaagacaa ccggaacgcc gattgccctg
    74641 gaggtgcgga atacagacca gagatcccac gattacagca atatcatgga tgtataccgc
    74701 cccggtcatg cagatttcac ctttgatcaa aagtatggct tcagggatta cagaggaggc
    74761 ggccgttcct ccggacggga gaccattggc cgggtagcag ccggagcagt ggccgcaaag
    74821 cttctggagc gccttggggt aagggttttt gcttatacca gcgcaatcgg ccctgtaagc
    74881 atcgaccggt cccggatgga gatttccaaa atgtgggaaa accgtcttta tatgcctgat
    74941 gacattgcgg caaaggaagc agagagttat ttagaggata tgatggctcg aagggattcc
    75001 tgcggcggtg tggtagagtg tgtgatcgag gggcttcccg caggcgtggg agagccggtg
    75061 tttgacaagc tggatgcggc tctggcaaaa gccatgatgt ccattggagc agtgaaaggc
    75121 gttgagatcg gagacggatt tgaggcggca gtatccaccg gttcacagaa taacgacagc
    75181 ttctgcattg acagtgcggg ccacccggcc aagcgaacca atcactccgg tggcgtcctg
    75241 ggagggatga gcgattccag cccggttgta atacgggcgg cctttaagcc tactccgtcc
    75301 attgcccagc ctcagtccac ggtggaccgg agcgggaaag aacgggagat cgagatccat
    75361 ggacgtcacg atcccattat cgttccaagg gcagttgtgg tggcagaagc catggcagcg
    75421 cttactgcgg cagatctgct gcttatgtcc atgacctccc ggcttgaccg gatggaggaa
    75481 ttttttgaga gaatgaggag aaaatgaaaa tgaaaattgc aatgggaaac gaccatacag
    75541 ccattgagct taaaaatatt attaaggagt ttgtagaggg aaaaggctac gaggttattg
    75601 atttgggtac caattctaca gaaagctgcg attaccctgt atatggtgaa aaggtaggac
    75661 gggctgtggc ttccaaagaa gcagatctgg gtatcgtgat ctgcggcacc ggcgtaggca
    75721 tttcactggc agctaataag gtaaagggcg tgcgcgcctg tgtatgcagc gaaccctaca
    75781 gcgcaaagct gtcccgtatg cacaataaca gcaacgtcct tgcctttggc gccagagtag
    75841 tgggaagcga gatggcaaag atgatcaccg aagcatggct ggacgctgag tttgaaggcg
    75901 gcag
//
LOCUS       DBMDUH010000003        57573 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_554_length_57573_cov_5.681358, whole genome shotgun sequence.
ACCESSION   DBMDUH010000003 DBMDUH010000000
VERSION     DBMDUH010000003.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 57573)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 57573)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57573
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_554_length_57573_cov_5.681358"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1458
                     /locus_tag="ACZPCG_00695"
     CDS             <1..1458
                     /locus_tag="ACZPCG_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HIR1283625.1"
                     /translation="MGQSYMIDKFPFLGNEIVRLIFSSLQSITSIIFNNIPLLFAMGV
                     AQGMAKQEKGIAVFASTVGYLALNIVMNVYLKATGTLADPAVAAQVGQGTVLGIQTLK
                     IEALGGLIAGLVAAKVADRFYRLELPLAFAFFGGKKSIPIITFVCMIPIGLVLPVIWN
                     ALTNFLISISFIFTAPYIGSSVYYTLNRALIPFGLHHVLASLVRFTEAGGTYMLNGQE
                     YVGILNATNEILFNLGPTSEYWTQLMPKLTSYLGGAQMLTTLFRVPAIGLAMYHTSYL
                     KNRKIAKGVILTCCLTAFLGNITEPLEFSFLFISPALFILYCVMCGIGLLPYQLLKIS
                     IGYIRGTIFDFGIFGLLYENTHWIGLVVLGIFNFIVFYFVFKWFILKFELETPGRESF
                     EVEESASILLKEKNWPAIAEIVIDGLGGKENILNVENCISRLRVDLSDPKKVDQVKLK
                     DSGCAGIFFPSEKHIHVVFGPHVEFVRHAVDDKLK"
     gene            1474..2865
                     /locus_tag="ACZPCG_00700"
     CDS             1474..2865
                     /locus_tag="ACZPCG_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class-II fumarase/aspartase family protein"
                     /protein_id="HIR1283626.1"
                     /translation="MRALYDSKSKLDDRGIKKLLSEENKYRTWLKIEQALAYSQAEEG
                     FIPKAAAKDIGSVAFADLDMEEMSRIKEKVGHGFVPFVKVLVRACRGEGKKYVHYGVT
                     TQNIQQTSQLYTAKEVTEIYKSFLADILEHLAALARENAHTVMAGRTHGKHAIPITYG
                     YKVSVWISELLTHLERFEEAEKRIFTVMMGGAVGGFNATGLVGRRVQEQVAERLNMSS
                     MEVPSRNMSQMKVEYLMNLCLLCNTFHKMAEEVYYTGIEEFSEVCEGFTPGTIGSSTM
                     PQKINPKLAKGIIANSQKLYSLPATGLYSAVRMFEGDSSSYMLFDGLMEEAMELTAEV
                     LIRAEELTRTLNVNKERMLENANINHGLDNSEYVMMKAAERLGKDKAHELLYDKAMKA
                     ELENKDYFTVLTEDETLSSMFTKEELKQLIDPASYTGLCSVLAVEMADKAEKAAAIIK
                     EEHKNQDSWEELK"
     gene            2862..4184
                     /locus_tag="ACZPCG_00705"
     CDS             2862..4184
                     /locus_tag="ACZPCG_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007716536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 4 glycosyl hydrolase"
                     /protein_id="HIR1283627.1"
                     /translation="MRESHIITIAGAGSGRVPALVGTLVQYKERFPLKKIIFYDIDEE
                     RMGKMEAYDRLVLKCYYPECEVVFTTDEDEAYRETDFVFCQMRVGKTRMRSYDEKLPL
                     KYGLVGQETCGPGGFAYGMRSLGAMKKMVEKVRSYSKDTWILNYTNPAAIVALGLDQM
                     FPDDKRILNLCDQPFSMMKSFAKILGVPQESLRARYFGLNHFGWFTELKDTEGKDHFE
                     ELRTYLRDHDFKPFNAEQRSKSWLDTYMRVNQYMKYFDEYIPTTYMQYYMFPQEIAAE
                     SDPNYTRADEARDSREKQVFDACALAEGRENMDGIEMLTNSVFGNLMVEVAESIAYDL
                     NREFIVLVRNEEIIPNFEKNAIIEVAGTIGKDGAKGYPYGEIPLFYKGLMEGQYAYEQ
                     LTVDAFREKNYEKALMALTVNRTVVDPGKAKAVLDDLMEVNREYWYLQ"
     gene            4200..5387
                     /gene="nagA"
                     /locus_tag="ACZPCG_00710"
     CDS             4200..5387
                     /gene="nagA"
                     /locus_tag="ACZPCG_00710"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007716533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HIR1283628.1"
                     /translation="MVLYSKHIILESEEFDGFLELEHGKIQGIHKSWEGSYEDYSNCV
                     ILPGFIDIHVHGWATGSFWYEKTEESLRKMCHTLPFSGVTSYLGTTGADSVEEIKRCI
                     HAADQVYEENEEGAELLGVHLEGPFINPKYRGMQKEECCLNPDIQVMKELYNTFRHKE
                     LCRHMTLAVELPGAREVLEFCREHRIQTAIGHSAATFQEIRDIKDVGIGGFTHTFSGM
                     KGFHHRELGVAGAALYFDDMFCEFAKQTGLTVSHEAFDIAFRIKGSERIILTTDCTGL
                     AQTQTEFEHYVRKLRFVKDGNKVRIEHFDGRTEWLDPEDYSAVKNVEMGYAESVKNMA
                     AHTKADWFDIMRMTSLNPARYIHVDDRKGKIAPGMDADLTIMDQDKNLICVYCRGREI
                     REA"
     gene            5384..5674
                     /locus_tag="ACZPCG_00715"
     CDS             5384..5674
                     /locus_tag="ACZPCG_00715"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1283629.1"
                     /translation="MKCAVSDFDRTLYVNGAVSERNIEAVKQWQRKGNLFFIATGRNL
                     ASIKEQLGFFDLEPDGLILNNGAAIMDKEGKLLLCRIIEKKQLLKYSGFFMN"
     gene            5617..6165
                     /locus_tag="ACZPCG_00720"
     CDS             5617..6165
                     /locus_tag="ACZPCG_00720"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1283630.1"
                     /translation="MPYHREKTALKILRFLHELNEDGSGVSMTDKKINVLSSSGTTTQ
                     KACDGVCRIDQIEGLESILQIHRRNKDEAYIRRLCKVINERFDQVSAFANVWNADIVA
                     KGVDKAAAVHWILNRRPDIDEVRVMGDSANDAGMIREFHGAAPVWASAEVRQTAAGVL
                     NDAAELLEDSCPSAVCFEFRKN"
     gene            6276..6746
                     /locus_tag="ACZPCG_00725"
     CDS             6276..6746
                     /locus_tag="ACZPCG_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007716538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIR1283631.1"
                     /translation="MGFLFGKKKSEFVSPAGGTLMDITAVADPAFSSKMMGDGFAIDL
                     TEGEVVAPFDAEVTAAFPTGHAYGLRRKDGVECLIHIGMDTVELNGEGFDVKVEQGAK
                     VKQGQTLVVVDLEAVKAKGKSLVTPVIFPSGAAFTMNKKDGIVAKAEENILKFS"
     gene            7003..8076
                     /locus_tag="ACZPCG_00730"
     CDS             7003..8076
                     /locus_tag="ACZPCG_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HIR1283632.1"
                     /translation="MKAYLILEDGTVFTGTSIGAEREVISEIVFNTSMTGYLEVLTDP
                     SYAGQAVVMTYPLIGNYGICREDMESERAWPDAYIVRELSRIPSNFRSEDTIQNFLKE
                     QDIPGISGIDTRALTKLLREKGTMNGMITTKEYTDHSEVLKRIRNYSVEGVVLKTTTK
                     EKYVLPGTGKKVALMDFGAKKNIARSLNERGCEVTVYPADTPAEEVLAANPDGIMLSN
                     GPGDPKENTAIIKEVKKLYDSNVPIFAICLGHQLMALANGMDTYKLKYGHRGGNHPVK
                     DLETGRVYISSQNHGYAVDNATISPEIAEPAFINVNDGTNEGLKYTGKNIFTVQYHPE
                     ACPGPLDSGYLFDRFLKMMEVND"
     gene            8076..11294
                     /gene="carB"
                     /locus_tag="ACZPCG_00735"
     CDS             8076..11294
                     /gene="carB"
                     /locus_tag="ACZPCG_00735"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HIR1283633.1"
                     /translation="MPKNPEIKKVLVLGSGPIVIGQAAEFDYAGTQACRSLKEEGIEV
                     VLLNSNPATIMTDKDIADRVYIEPLTVEVVEQLILKEQPDSILPTLGGQAGLNLAMEL
                     EDAGFLREHNVRLIGTTALTIKKAEDREMFKETMEKIGEPVAPSDIVEDVKTGLEIAA
                     KIGYPVVLRPAYTLGGSGGGIAHNPEQCAEILENGLRLSRVGQVLVERCIAGWKEIEY
                     EVMRDGAGNVITVCNMENIDPVGVHTGDSIVVAPSQTLGDKEYQMLRTSALNIISELG
                     ITGGCNVQYALNPDSFEYCVIEVNPRVSRSSALASKATGYPIAKVAAKIALGYTLDEI
                     KNAVTKKTYASFEPMLDYCVVKMPRLPFDKFISAKRALGTQMKATGEVMSICTNFEGA
                     LMKAIRSLEQHVDCLLSYDFTELSYTELVNDLYKVDDMRIWRIAEALRRGFPYGLIHE
                     ITRIDFWFIDKLAILVEMEKELQAVGERKKELTPELLAEAKRIEYPDNVIARLTKMEQ
                     SAVKKLRYDNNIRAAYKMVDTCAAEFAAETPYYYSCFGSFNEAERTEGRKKVLVLGSG
                     PIRIGQGIEFDFCSVHSTWAFSKEGYETIIINNNPETVSTDFDIADKLYFEPLTPEDV
                     ESIVDIEKPDGAVVQFGGQTAIKLTEALMKMGVPILGTAAEDVDAAEDRERFDAILEQ
                     CGIPRPAGHTVFTAEEAKEAAHKLGYPVLVRPSYVLGGQGMQIAINDEDIDEFIGIIN
                     QIAQEHPILVDKYIMGKEIEVDAICDGTDILIPGIMQHIERTGIHSGDSISVYPAQDI
                     TEKNVETIVDYTAKLARALHVKGMINIQFIVDGEDVYIIEVNPRSSRTVPYISKVTGI
                     PIVPLATQIICGHTIRELGYTPGLQPAADYIAIKMPVFSFEKIRGADISLGPEMKSTG
                     ECLGIAKTFNEALYKAFEGAGIRLPKYKKMIMTIRESEQEEAVEIARRFIAVGYQIFA
                     TRGTARTLNAHGVKAYEIRKLEQESPNILDLVLGHQIDLIVDIPAQGAERSHDGFIIR
                     RNAIETGVNVLTALDTARALVTSLENRAKDLTLIDIATVKNA"
     gene            complement(11428..12360)
                     /gene="arcC"
                     /locus_tag="ACZPCG_00740"
     CDS             complement(11428..12360)
                     /gene="arcC"
                     /locus_tag="ACZPCG_00740"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009532603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019546 - L-arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HIR1283634.1"
                     /translation="MAKKRIVMAIGHKDMGTNLPEQKEAVQRTAKVIADFIQEGWQVA
                     IVHSNAPQVGMIHTAMNEFGRQHDGYSSAPMSVCSAMSQGYIGYDLQNGIRAELIRRG
                     IYKPVSTVLTQVTVDPYDEAFYTPVKVIGRVMNREEADEEEAKGNHVKEVEGGFRRIV
                     ASPHPVAIVEIDAIKALLDADQIVIACGGGGIPVMEQGYNLRGASAIIEKDLATGLLA
                     KETDADVMMILTSVDNVTLNYGTDHEEPIKNMTIDQAKDYISQGQFEFASMLPKISAS
                     VDFLECGKGRKAIITSLDKAEEALKGEAGTTING"
     gene            12476..12961
                     /locus_tag="ACZPCG_00745"
     CDS             12476..12961
                     /locus_tag="ACZPCG_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HIR1283635.1"
                     /translation="MQQNTDILRNTQMVEKKSFLPPGWQRLLLGTVLAALAGNILVRL
                     IPWPESWLISYGEVQTAERMSQSYYLALTLAAAPVMEEAVFRLGIYGLLRRKLRPKAA
                     AFLSALAFGFYHGNMIQGLYAFLLGFVLAWGYEKCPWGKYRMAVLMHAAANGAALLAF
                     G"
     gene            13007..14026
                     /locus_tag="ACZPCG_00750"
     CDS             13007..14026
                     /locus_tag="ACZPCG_00750"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1283636.1"
                     /translation="MKLLSVAIPCYNSEAYMEHCIRSLLTGGDEVEILIVDDGSTKDR
                     TAEIADEYEKRYPGICRAIHQENGGHGEAVNAGLRNATGVFFKVVDSDDWVNEEAYQK
                     VLETLRRFVYRGEALDMLVTNFVYEKEGARRKKVMKYHTAFPKDCVFGWKDVKFFMIG
                     QYILMHSVIYRTQLLKECGLELPKHTFYVDNIFVYQPLPHVKNMYYLDVNFYRYFIGR
                     TDQSVNESVMIGRIDQQLRVTRLMLGYYDATKISNPKLRHYMIQYLEIMMTVCSVLAI
                     KSGTAENLAKKKELWNELKKQNFPLWLRLRWGFLGQGCNLPGKGGRELLILGYKITQK
                     FYGFN"
     gene            complement(14124..14789)
                     /locus_tag="ACZPCG_00755"
     CDS             complement(14124..14789)
                     /locus_tag="ACZPCG_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR1283637.1"
                     /translation="MKRVCDFFRKYSHIWLLSYFFIYIPWFCYLEKTVTRDYHLMHAP
                     LDSLIPFNEYFIIPYLLWFAFVGVTLIYFLIKSKEDYYRMCTFLFSGMTISLIICTFF
                     ENGTNLRPILDPDKNIFTAAVAALYRTDTCTNVFPSIHVYNSIGTYIAIHKSPLFQGK
                     TALKAGAAILTAAICLSTVFLKQHSVIDGVGAGIMAAMMYHLVYACSPVEEEKKIVEK
                     ILE"
     gene            15022..15882
                     /locus_tag="ACZPCG_00760"
     CDS             15022..15882
                     /locus_tag="ACZPCG_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006778905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HIR1283638.1"
                     /translation="MSEARSVLGVICSFYDSFFEAEKKIADYIMEHKAQAVDMTVGEL
                     ARASGTSDATVSRFCRRCGFKGFQSLKLALAREVLREEQDSRKVTNDIDRENLSQSLN
                     NILANKVAELTETVRMMDIHSLEEILKKLEKARMVQLAAVGNTIPVALDGAFKFNQLG
                     IPSVAGDIWETQAAYTFNLGPEDVVLLISNSGFSRRLNTLAQGAKENGSTLVLITNNP
                     DSPLASICDHKIITATREKLLTEEFWFSRVTATAVIEILYLLLRAGKKDAMEHIRRHE
                     EAISSDKNEI"
     gene            16057..16977
                     /gene="pfkB"
                     /locus_tag="ACZPCG_00765"
     CDS             16057..16977
                     /gene="pfkB"
                     /locus_tag="ACZPCG_00765"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HIR1283639.1"
                     /translation="MIYTVTFNPALDYVIAVDHFAQGKVNRTVKEDIFCGGKGINVSV
                     LLANLGVESTALGFVAGFTGKEIERGLKAFGISSDFIHVKSGMSRINVKLKSEEETEI
                     NGMGPDIRPEDVEALFEKLNRLGSGDVLVLSGSIPAAVGNDIYRRIMEQLKDRGVCIA
                     VDAERDLLTEVLPLKPFLIKPNNHELGQIFGKELKTEKEIIDGAMSLKDRGAVNVLVS
                     MAKDGAILVDENGKVHRLGVCRGTVKNSVGAGDSMVAGFLAGYLKTGDYDYALRLGTA
                     SGGATAFSDGMGSRKMIMDLFAQLTENGGK"
     gene            16981..18906
                     /locus_tag="ACZPCG_00770"
     CDS             16981..18906
                     /locus_tag="ACZPCG_00770"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002578059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HIR1283640.1"
                     /translation="MRITELLKKQSIELGVKAASKEEAIDRLIALMDAGGRLNDRAGY
                     KEGILARESLGSTAIGEGIAIPHAKVEAVKEPGLAAITVPEGVDYEAFDGSLSNLIFM
                     IAAPAGGEDVHLEALSRLSTLLMNPGFKEALIHAGSKEEFLEVIDQAETERFGKKEEM
                     DEPEKAAEPEKTGFRVLAVTACPTGIAHTYMAAENLENTGKKLGISLKAETDGSGGAQ
                     NILTKEEIREAEAIIVAADKNVEMARFDGKPVIMVPVADGIHKAEELIRRAVSGTVPV
                     YHHEGGTSEAEAEGDKSIGRTIYKHLMNGVSHMLPFVIGGGILIALAFLLDDYSIDPS
                     NFGKNTPLAAYLKTIGEQAFGMMLPILAGFIAMSIADRPGLAVGMVAGMVAKMGCTFA
                     NPAGGDVNAGFLGALFAGFAGGYLVLGLKKIFSRLPKSLEGIKPVLLYPVLGIFVAAV
                     MTTLINPYMGMINDGLTHFLNGMGGASRIVLGMVLGAMMSIDMGGPFNKAAYVFGTAQ
                     LAEGNFEVMAAVMAGGMVPPIAIALCTTFFKKKFTEKERQSGIVNYIMGLSFITEGAI
                     PFAAQDPLRVIPACGIGAAAAGGLSMAFGCTLRAPHGGIFVLPTIGNPLMYLAAVMGG
                     AVAGCLILGLLKKNTEE"
     gene            complement(18967..19389)
                     /locus_tag="ACZPCG_00775"
     CDS             complement(18967..19389)
                     /locus_tag="ACZPCG_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HIR1283641.1"
                     /translation="MYAEFTDDLITGNEMIDSQHRELISRINDLLVSCENRSNQSGAA
                     RLLNYLADYTDFHFKEEEAFQKEIGYPGLKEHLEKHEELRRTVQELHDMLTDEEGPSD
                     AFVSKVAENVRDWLYYHIKGFDRSVAEYKYMRDSADRI"
     gene            complement(19526..19599)
                     /locus_tag="ACZPCG_00780"
     tRNA            complement(19526..19599)
                     /locus_tag="ACZPCG_00780"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19563..19565),aa:Met,seq:cat)
     gene            complement(19618..19690)
                     /locus_tag="ACZPCG_00785"
     tRNA            complement(19618..19690)
                     /locus_tag="ACZPCG_00785"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19655..19657),aa:Val,seq:tac)
     gene            19980..20936
                     /locus_tag="ACZPCG_00790"
     CDS             19980..20936
                     /locus_tag="ACZPCG_00790"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002571899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIR1283642.1"
                     /translation="MDERKGPYAPMYYFIVNPSAGSGRGRRVWKAIACYMDLHNIEYE
                     AVLTGSVGDARIAARELTEKKRQPGLLIVVGGSGTVNEVLDGAVLHGIFDLGYIPAGT
                     GNDLSRCLHIPKRTVRCLKKQLYPRHFSMVDYGVLSLGSSEISHRRFMVSAGMGFDAA
                     VCCAASASRLRGIMGRLGLGKLFCFLLGLCQAAGCRSSRGYILLDGVKKVEFNHILFI
                     SCHIQPSEGGGFVFAPRADGSDGRMNVCVVSHSSRKRLIPVMLQALTGRFKKHKGARM
                     FECREVSIHTDAPLPIHVDGEFCGTQTDLRAECISRQIRMGI"
     gene            21013..21561
                     /gene="ispF"
                     /locus_tag="ACZPCG_00795"
     CDS             21013..21561
                     /gene="ispF"
                     /locus_tag="ACZPCG_00795"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006779536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HIR1283643.1"
                     /translation="MRIGQGYDVHRLTEGRKLILGGVEIPYEKGLLGHSDADVLIHAV
                     MDALLGAAALGDIGQHFPDTDPAYKGISSIELLGKVGALLEEKGYVIENIDATIIAQR
                     PKLASYRPQMEENIAEALHLDPSRVSVKATTEEGLGFTGSGEGIASQAITLLTETADY
                     CYDNKIMTEGCPGCSGCGCEQK"
     gene            21600..23009
                     /gene="cysS"
                     /locus_tag="ACZPCG_00800"
     CDS             21600..23009
                     /gene="cysS"
                     /locus_tag="ACZPCG_00800"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006779537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HIR1283644.1"
                     /translation="MKIFNTLTRRKEEFKPITPGEVKMYVCGPTVYNLIHIGNARPMI
                     MFDTVRRYFEYKGYKVKFVSNFTDVDDKIIKKANEEGVDAHIISERYIAECKKDMKDM
                     NVEPATVHPQATQEIQGMINMIQTLIDKGHAYVAEDGTVYFKTRSFAEYGKLSHKNLD
                     ELQSGFREIKVTGEEGKEDPNDFVLWKPKKEGEPYWESPWCQGRPGWHIECSVMAKRY
                     LGDSIDIHAGGEDLIFPHHENEIAQSECANDKPFAAYWMHNGFLNIDNKKMSKSLGNF
                     FTVRDIAEKYDLQVLRFFMLSAHYRSPLNFSAELMEASKNGLERIMTCMDKLRELKAK
                     AQAGDMTAAETEKMAEADRLREKFEAAMEDDFNTADAVSAVFELVKLANSTADADSTA
                     AYVKGLEDLISRLCEVLGIITERKTEVLDEEIEAMIAARQQARKEKNFALADEIRGKL
                     LDMGIVLEDTREGVKWKRA"
     gene            22994..23449
                     /locus_tag="ACZPCG_00805"
     CDS             22994..23449
                     /locus_tag="ACZPCG_00805"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006779538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HIR1283645.1"
                     /translation="MEESITLKDFLAYYKKTMRLEPVDAGSYSPLVLAYIGDAVYEVM
                     IRARVINRGSMQVNKMHKRSAMLVNAGTQAQLIRSIEEELTPQEHAVYKRGRNAKSVT
                     MAKHATMADYRTATGMEALVGWLFLEERFERLAELISLGLEKIGAFEEI"
     gene            23464..24207
                     /gene="rlmB"
                     /locus_tag="ACZPCG_00810"
     CDS             23464..24207
                     /gene="rlmB"
                     /locus_tag="ACZPCG_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HIR1283646.1"
                     /translation="MRYEELTIEGRNAVLEAFRSGKTIDKLFVLDGCQDGPVRTIVRE
                     AKKHDTIINYVAKERLDQLSETGKHQGVIAYAAAYEYAQVEDMLKLAEEKGEPPFLFL
                     LDGIEDPHNLGAIIRTANLAGAHGVIIPKRRAVGLTATVAKTSAGALNYTPVAKVTNL
                     TAVMEDLKKKGLWFVCADMGGDVMYRMNLTGPIGLVIGNEGEGVSKLVREHCDMTASI
                     PMKGDIDSLNASVAAGVLAYEIVRQRLAK"
     gene            24231..25199
                     /locus_tag="ACZPCG_00815"
     CDS             24231..25199
                     /locus_tag="ACZPCG_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006443741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HIR1283647.1"
                     /translation="MERIRKNRRNISGAAVLTAAVVGVCGLFGAFPAKASQTAVIEDT
                     QVPLYDKPAGSYVRTPIASGVTVYKNDKATLDASNTSEGYLMVKYTGSVGKIKVQISK
                     NGSETYTYDLNNSGVYEVFPLSEGNGSYQVKVFENIQGNQYSQAFSQNLDVSISNPFG
                     PFLYPNQYVNFNSASAAVQKGAELSAGVTDQLGVVSAVYNYVVKNLTYDTAKASSVQS
                     GYLPNVDLVLAQKKGICFDYAALMTAMLRSQDIPTKLVVGYTGNLYHAWINVYLEGQG
                     WVDNIIYFDGTSWKLMDPTFASSGNQSQEIMQYIGNGANYQAKYSY"
     gene            25217..26275
                     /locus_tag="ACZPCG_00820"
     CDS             25217..26275
                     /locus_tag="ACZPCG_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002571901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR1283648.1"
                     /translation="MEKQGEKYDRYSYEELSAFCLQIALLLEAAVPLEEGLTIMAEDA
                     AEEKEKAMLLYMAEGAELGDPFFKVLEDTGVFPPYIVRMARLGQQTGTLDQMMKSLSD
                     YYEKEARLLKAVKNAATYPAMMILMLLVVLFVLFTKVMPVFSRVYEQLGASLPPAAAA
                     AIRLGGILSGAALVLAAVTGAAAAVIWIAARFGKRFSLVEKGVAAVKNRSRIARAVAL
                     RRFTSVLALTLKSGLELEKSMELAKELAENETVAGQIDDCSRRLEEGESYYDAMKAAG
                     LFSGFYIQMIKVGSRSGHLDQVMEEISGDYEEMADSAIDGMIARFEPTIVAVLAVSVG
                     LVLLSVMLPLVGVLSAIG"
     gene            26357..26620
                     /locus_tag="ACZPCG_00825"
     CDS             26357..26620
                     /locus_tag="ACZPCG_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007709027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1283649.1"
                     /translation="MMAAILFFTLFSVQFGERTTARDQETLKKAIARASVQCYAIEGR
                     YPPSIEYLEEHYGVMINRKKYNVFYDGFASNVMPDITVVPVME"
     gene            26632..27162
                     /locus_tag="ACZPCG_00830"
     CDS             26632..27162
                     /locus_tag="ACZPCG_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007709028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4860 domain-containing protein"
                     /protein_id="HIR1283650.1"
                     /translation="MERKTGKRGQLMGGLFTMLLFLVFILSALFTVLTGSRVYENITA
                     RSDKNFSSSTALSYIANKVRQGDRAGMTDIKEIEGTQVLELRQQIGGTDYVTWIYWLD
                     GSIRELFTDTGSGLGLEDGLEILECEGLSFSREGNVLHIRTEGENGGTLSLSLRSGSL
                     AENPVFGESESGGIEG"
     gene            27159..27569
                     /locus_tag="ACZPCG_00835"
     CDS             27159..27569
                     /locus_tag="ACZPCG_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1283651.1"
                     /translation="MNRRSGSGSGPFLMEMLAVVGFFIVCASICVSVFAGADRLSRKA
                     DDLNHAVLAAQSLMEELKSGKREEELQTVWDKDWKAYTQREAAESGAVLAYEAEITVE
                     MDDSMKQIRVDVTKGDKETDGTVIYSLEGSQYEP"
     gene            27595..28068
                     /locus_tag="ACZPCG_00840"
     CDS             27595..28068
                     /locus_tag="ACZPCG_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002593616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1283652.1"
                     /translation="MAEQTSRNKVNIGLSSLILIFIVLCLSTFGLLSLSSARGDLELA
                     ERGAQAAKEFYEADSKGQQWLAGSDRMLQQMAGRSLEEKLEALAAKPGNGYDEQTGLI
                     FTEIPMERGESLHIDLELKEGDKRYEIRSWYVYDSGEYEIDDTVKVWDGKDRSGT"
     gene            28084..29145
                     /locus_tag="ACZPCG_00845"
     CDS             28084..29145
                     /locus_tag="ACZPCG_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HIR1283653.1"
                     /translation="MKVMELLTAAVEQEAADIFLIPGRPFSYKIGGRIICEGTEKIMP
                     DEMDDMIRQIYELAGGRSEERVLNEGDDDFSFAVPGVSRFRANIFRQRGSLAGVIRVV
                     RFELPDADALHLPKEIMDVSRMTKGMVLVTGPAGSGKSTTLACIIDAINESREAHVIT
                     LEDPIEYLHRHKKSVVTQREIATDTSSYVTGLRASLRQAPDVILLGEMRDYETISIAM
                     TAAETGHLILSTLHTIGAANTIDRVIDAFPPAQQQQIRTQLSMVLNAVISQQLVPTVD
                     GGVQPVFEIMFLNNAIRNMIRESKIHQIDGIIATSQEEGMISMDNSLIRLFRQGVISR
                     ETAVTYSSNGELMERKLSR"
     gene            29224..30168
                     /locus_tag="ACZPCG_00850"
     CDS             29224..30168
                     /locus_tag="ACZPCG_00850"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HIR1283654.1"
                     /translation="MRSDKRKVAVVGTGMVGMSYAYSLLNQSVCDELTLIDINKTRAE
                     GEAMDLNHGLAFAASNMKISAGNYEDCRDADIVVIAAGVAQKPGESRLDLLRRNTEVF
                     RSIIEPVISSGFNGIFLVATNPVDIMTRITCVLSGFNPGRVLGSGTALDTARLRYLVG
                     GYLRVDPRNVHAYVMGEHGDSEFVPWSQAFLATKPILELGKNGKNELRSCLDHIEEEV
                     RTAAYRIIEAKQATYYGIGMALTRITKAILGDEHSVLTVSAMLRGDYGQRDIYTGVPC
                     IINQNGVQRVLELSLSEQEMEKFGKSCKTLRESFEGIV"
     gene            30289..30837
                     /locus_tag="ACZPCG_00855"
     CDS             30289..30837
                     /locus_tag="ACZPCG_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006771061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HIR1283655.1"
                     /translation="MPLLKERYTSKDYWALPEGCRSELIDGQLYDMAPPSRIHQEIVS
                     GILYLLKKHIESKKGGCKVYPAPFAVNLDADDKNWVEPDISVICDPDKLTDRGCSGAP
                     DFIVEVVSPGSRRMDYAHKVSLYLESGVREYWIVDPLKKCTTVYLNEQDPSEFAPIIF
                     PFESNIKVGIYADLSINISQLL"
     gene            complement(30923..30995)
                     /locus_tag="ACZPCG_00860"
     tRNA            complement(30923..30995)
                     /locus_tag="ACZPCG_00860"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30960..30962),aa:Thr,seq:ggt)
     gene            complement(31091..31432)
                     /locus_tag="ACZPCG_00865"
     CDS             complement(31091..31432)
                     /locus_tag="ACZPCG_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter permease"
                     /protein_id="HIR1283656.1"
                     /translation="MIHSSQNTSYIIITLLICVLCTQATRWFPFLLFGGKRQLPELIR
                     YLGTVLPPAIMAVLIVYCLKGVTPLTWPHGLPELIASAVTAGAHLWKKNTLISIFTGT
                     LCYMILIQKIF"
     gene            complement(31429..32133)
                     /locus_tag="ACZPCG_00870"
     CDS             complement(31429..32133)
                     /locus_tag="ACZPCG_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002571907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HIR1283657.1"
                     /translation="MKKAAGFALIKTIPVFLGYIFLGIAFGLLLQKAGLGAGWVFLIS
                     LFVYAGSMQFALIGILTEGLSFAATAIMTLLINSRHAFYGLTFIERFRKMKAAGPYMI
                     FSLTDETYSILCSAKKPDDFTDREWKLSSLFISLFNHCYWVAGSVAGALIGQFLPFDT
                     TGIDFAMTALFIVICVNQWRETSCHLPAKTGFFCGMLCLALFGPSGFILPALIAVSAL
                     LLIFRHTIQRNMEDFS"
     gene            32346..34040
                     /locus_tag="ACZPCG_00875"
     CDS             32346..34040
                     /locus_tag="ACZPCG_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HIR1283658.1"
                     /translation="MAEKDMVLETGEKETVSKNFIEQEIDKDLAEGVYDNVHTRFPPE
                     PNGYLHIGHAKSILLNYGLAQKYDGTFNLRFDDTNPTKEKVEFVESIKADVKWLGADF
                     ENRLFFASDYFEKMYECAVFLIKKGKAFVCDLSAEEIREYRGDFNNPGKESPYRNRSV
                     EENLRLFEEMKEGKYKDGEKVLRAKIDMASANINMRDPVIYRVAHMEHHNTGNKWCIY
                     PMYDFAHPIEDAIEHITHSICTLEFEDHRPLYDWVVKECEFENPPRQIEFAKLYLTNV
                     VTGKRYIKKLVEDHIVDGWDDPRLVSIAALRRRGYTPESIRRFVELVGVSKANSSVDY
                     AMLEYCIREDLKLKKARMMAVLDPVKLVIDNYPEGQMEELDVPNNLENPELGSRKVPF
                     GRELYIEREDFMEEPPKKYFRMFPGNEVRLMGAYFVKCTGCEKDADGNVTVVHGTYDP
                     ETKSGSGFEGRKVKGTIHWVAVPTAKKVECRLYENIVDEEKGKLNEDGSLNLNPNSLT
                     VLPDCYVEPALGEAESYDSFQFVRNGYFCVDCKDSTKEKPVFNRIVSLKSSFKLPK"
     gene            34171..35571
                     /gene="pdxR"
                     /locus_tag="ACZPCG_00880"
     CDS             34171..35571
                     /gene="pdxR"
                     /locus_tag="ACZPCG_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070279 - vitamin B6 binding [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:2000142 - regulation of DNA-templated
                     transcription initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MocR-like pyridoxine biosynthesis transcription
                     factor PdxR"
                     /protein_id="HIR1283659.1"
                     /translation="MELMVPIDNQSRLPLYEQIYQYMKEEIQSGRIPAGSRLPSTRIL
                     AENLKVSRSTTQMAYEQLLSEGYIEALPCKGYFVCRIEELVRVEKNADEEFAENRSEK
                     EMPYAVDFSTRGIDLDSFPFNTWRRLSRNTLVDDNKEMFAVGDPQGEYELRAAIRDYL
                     HSARGVSCRPEQILVGAGSEYLLMLLSQLLGRETGIALENPTYRQAYRVLKSLGHLVV
                     PVKMDRYGMDVLDLEQSKTQAAYVMPSHQYPTGIVMPVKRRQELLSWAYRGENRYVIE
                     DDYDSEFRYKGRPIPALQGMDQGGRVIYMGTFSRSIAPAIRVGFMVLPGELLSRYRSQ
                     AGFYACTVSRIDQKVLYHFITGGYYERHLNRMRAVYKGKHDALLSGLKELEDMFWIRG
                     EYAGIHVLLTHKGQMPEKELIERAAGEGVRVYGMSEAVIGENKDLVPSTVILGYANLK
                     EKEIQEGCMRLARAWK"
     gene            35617..36498
                     /locus_tag="ACZPCG_00885"
     CDS             35617..36498
                     /locus_tag="ACZPCG_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1283660.1"
                     /translation="MKRKRKERYRLWAAVAVLYAVVLPVSGLMGMTENRQNARMQSES
                     VREVEPVNKQGAVKENEENPTGSETVFGELSLTAEQLDAAEAVFSSLESLDLEQAARL
                     MDREQDVLADLFYETMGGTRYLYTPSGFTAQIEGEGMVFTMPGTVFYGYFKDGKPRGE
                     CLALQFINVDSPRYNYSYGIWRNGRMTGYGSTGYCYYENVPQGEMAKTVKQGNFKNDL
                     LEGEISYETTGADGTVSHWYMAASEGATVIDTRWTWMEEEKEYQLMSADAQSHGYFIS
                     EEQAGQVMWMNLLGWEV"
     gene            complement(36550..37212)
                     /locus_tag="ACZPCG_00890"
     CDS             complement(36550..37212)
                     /locus_tag="ACZPCG_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1283661.1"
                     /translation="MAKKGWGKLVALAAVSGAVAAGVSYVLQYKTYHKELEKDFREFE
                     EDEDSELKKDGSEDSTFESAKVNRNYISLTSSKDEFKVAAKDMAQATKNVLRDAGSLF
                     SDTAHEAVSAAVDTAHIALNSMKSKKEEWKEEHEKEDEPEEVHMEEDGFLDDDYVDDD
                     DLYDYERLDAEPVYAEKQPSETEDGGSSEEDDDETEDVVSVELAEDEEGTILTEDDEP
                     LV"
     gene            complement(37353..38147)
                     /locus_tag="ACZPCG_00895"
     CDS             complement(37353..38147)
                     /locus_tag="ACZPCG_00895"
                     /inference="COORDINATES: protein motif:HMM:NF014376.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1283662.1"
                     /translation="MRAFFYSGAFNPRLFFYPLSAGHFYCTSGYRVDRSSFDSILIAY
                     IVRGTFSFLYQGRELTAKAGEIALVDCFGPHMYCTHNHLETYWLHISGSNTKELFEEL
                     MIKRGCIISPASVIPAHICELFELIKAREAISISRLSSRIYTLITGLFEETMDSEGEN
                     SLSFAAVRYMKAHYSEKITVEHMAKAVSLSPSQFSRQFKRQTGVPPYEYLLFLRLSKA
                     KELLKTTELPVHEIACQCGFGGESSFISFFKKQEGIPPLKFRTVLF"
     gene            38321..39910
                     /locus_tag="ACZPCG_00900"
     CDS             38321..39910
                     /locus_tag="ACZPCG_00900"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014850.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HIR1283663.1"
                     /translation="MKCITELKDWDFKIPELGFEKRVTLPYTWNVENERPVQLYRGPA
                     EYITEIELDEMPWDRAVLYFGCVFHTARVFVNGIPAGGHTGSGYTPFELDITDLAVRG
                     KNQICVVADNSKKEDMLPHGLDYDWADDGGLLRDVCLIRMEKDDIMEMKTGYAIESIS
                     GQTCSGYLEFYIKGTAKEALVQVSEYKTGHIVLSETIHTDTLVRIPFEGLKLWAPEHP
                     RLYVVQVRTKQDMASERIGLRTIEVKGAGVYLNGKRIFMKGCEWMPGSHPDYGMAELF
                     EHSRKCLSQLKQAGCVFTRFHWQQDRKILDWCDENGLMVQEEIPFWGQPKKAGSEQLA
                     LAKQQAGEMVHYHGSHPSIICWGVGNELGGELPETIAYVKEMYRYFKELDGSRLVNYV
                     SNTVSRDANVDLDDAAMYGDIAMWNEYLGLWQPCEDVEGVLRRTYEKFGGMPSMVTEF
                     GLCEPAFEGGDERRAQILRERIPVYKSLEKMAGYVWFSLNDYRTHYGEYGEGKMKQRI
                     HGSTDLYGNEKPSYEVFCEIE"
     gene            40109..40393
                     /locus_tag="ACZPCG_00905"
     CDS             40109..40393
                     /locus_tag="ACZPCG_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008721713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HIR1283664.1"
                     /translation="MKLVPLFDKVVLKQLVAEETTKSGIVLPGAAKEKPQQAEVIAVG
                     PGGVVDGKEVVMQVKAGDTVIYSKYSGTEVELDDEKYVIVKQSDILAVVE"
     gene            40439..42061
                     /gene="groL"
                     /locus_tag="ACZPCG_00910"
     CDS             40439..42061
                     /gene="groL"
                     /locus_tag="ACZPCG_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008721716.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HIR1283665.1"
                     /translation="MAKQIKYGVDARKALEAGVNQLADTVRVTLGPKGRNVVLDKSFG
                     APLITNDGVTIAKEIELADPFENMGAQLIKEVASKTNDVAGDGTTTATVLAQAMVNEG
                     MKNLAAGANPIILRKGMKKATDAAVEAIKAMSKPVGGKAQIARVAAISASDDEVGTMV
                     ADAMEKVSKDGVITIEESKTMKTELDLVEGMQFDRGYLSAYMCTDMDKMEANLDDPYV
                     LITDKKISNIQDLLPLLEQIVKMGARLLIIAEDVEGEALTTLIVNKLRGTFNVVAVKA
                     PGYGDRRKEMLQDIAILTGGTVISEEVGLDLKDATLDQLGRAKSVKVQKENTIIVDGE
                     GDKEAIAARVAQIRKQIGETTSEFDKEKLQERLAKLAGGVAVIRVGAATETEMKEAKL
                     RMEDALNATRAAVEEGIIAGGGSAYAHATVDVQKVVDGLEGDEKTGAKIILKALEAPL
                     FHIVSNAGLEGAVVVNKVKESEVGCGFDAYNEKFVDMIEAGILDPVKVTRSALQNATS
                     VASTLLTTESVVAAIKEPAPAAPAAPDMGGMY"
     gene            42278..43768
                     /locus_tag="ACZPCG_00915"
     CDS             42278..43768
                     /locus_tag="ACZPCG_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HIR1283666.1"
                     /translation="MKPYRWKRVLGICTAAVLGLSFPAYAVTITPLNPGTGQSSSSEP
                     SGTGAPASQENTERLNIAQPVVSSEGAVLMDGASGKVLFSKNGDTRYYPASITKLMTA
                     LLVAENCSLDDTVTFSASATTNLESGAVSIGMVEGDTMTVRQCLYALLLKSANEVGNA
                     LAEHVSGSVQAFADRMNEKAVKLGCTNTHFTNPHGLNDPEHYTTPKDMALIARAAFQN
                     EVVRTVASTRTYSLPATKKNPSGLTVTMGHKMLNPNDSRYYEGIIGGKTGYTSKAGNT
                     LVTAAERNGVRMIAVVMKSRSTHYVDTKAMLDYGFELAKAGALETNQTGGQNAGSSQT
                     GAGSGGPGAGAGPSGAAGAPESTGASGVTVVPGNTGAPQNPGSTGVPVTGTEGGPGVS
                     SQHGWAKTEKGWNFVKDNGAKASGEWVSDAEGTYWIDEDTFMAKGWRQIDGKWYYLRS
                     NGAMAKNYWEESQDGVWYYLGADGVMMTNATTPDGYRVGADGAWIR"
     gene            complement(43884..45650)
                     /locus_tag="ACZPCG_00920"
     CDS             complement(43884..45650)
                     /locus_tag="ACZPCG_00920"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF013718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase"
                     /protein_id="HIR1283667.1"
                     /translation="MKRSKFIKQAGALGAALAMTATPVMAATDPAAIEPVPGPHGYFV
                     DTYKTNAMENQSPESNAVVGVLSPFLNIWEPGSSWDNGTKLGEDGKRLLDQNIFTCIT
                     MTQNRTLEQEVQAYLTDRQNQNWSALDGLGSYETAFKNLANAKTSLPDAIPADAETKK
                     YSDKGNENGSWADETSSLGSMVELVNTVRGPHASGNPAKVYFQYMRPFRWSSDVSIVP
                     ALKPCVSSKPMEDGGFPSGHTNAGYLASLSLAYAVPEQFKECLTNASEIGNYRIIAGM
                     HSPMDVMGGRTMAMALAAAALNDPDNAQLKADARKEAEEILLKNPSAKEVTYYDDDEL
                     NAIRYNERMTYNMPQTGDPTQPMRVPKGAEVLLETRFPYLNADQRRWILYSTGLPSGY
                     VFLDDAEGWGRLNLYEAGDGYGSFDTDVTVTMDGSKGGFDAFDQWKNDIAGEGSLTKN
                     GSGTLELWGNNSYTGGVHVNGGTLTAASPYAFGYSSVDNHAAITENVNGALNISGDFT
                     QGAEGDLTLKISGPEDMLAVGGIVNAGGDLILDFTNYSQPASGMTVLTADQINGSFAS
                     VKTVGLADGKTVEVTDQAVIVK"
     gene            complement(45785..46726)
                     /locus_tag="ACZPCG_00925"
     CDS             complement(45785..46726)
                     /locus_tag="ACZPCG_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006773817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIR1283668.1"
                     /translation="MKTYHNSPLLYEEGFSIIINEVATPIENSTFYYFDYDKRSHAIN
                     MDFPHFHSFYELMILLSPKAYHFVEGKRYDIIANDLVLLPPSVLHQSEYLPGPPSDRI
                     IIGFMLPKQMNYGASGYKEILSVFNSSVPIFRFHKEEQNRLYQKLNEIVMIAKDTPNV
                     NIRNLLIHTKFTEFLFLLYEMQGLNHYVPNVENGVKEKIYTITNYIHTHYTEDISLAA
                     LAETFYISPYYLSHQFKNVTGYTVVQYIQLTRIKNAQYLLLNSPMRITQVAESTGFSS
                     FSQFNRVFRKFCGMSPSDYKLTSHHSSYRPTAPDEQQ"
     gene            46927..49959
                     /locus_tag="ACZPCG_00930"
     CDS             46927..49959
                     /locus_tag="ACZPCG_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009244576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HIR1283669.1"
                     /translation="MNYLHVNTEPDRAYYIPASGKGCYFLDREKSDRFLLLSGSWQFQ
                     YFESVYDLPQEFWKEGAGKGKEVQVPAVWQSYGVDGNQYINTRYPFPFDPPYVPRENP
                     CGLYERSFEYKCCLDAPEAFLNFEGVDSCFYVWINGIFVGYSQVSHSTSEFHITRYLK
                     EGENRLTVLVLKWCDGSYLEDQDKLRMSGIFRDVYVLRRPKEAVRDYFIRPYIRENGA
                     EVCMELSFYGNSRPDGRIRVLDPEGKAVWEQSWNETADRDQIRFYLENCRLWTAETPW
                     LYTVEIQTEKEWITDRFGIREISVENGVLFINKTPVKFHGVNRHDSDPVTGYAISRQQ
                     MERDLKLMKEHNVNAVRTSHYPNSPQYYHLFDEMGFYVMDEADNESHGTDKVFKKVDD
                     WDTHVKKWNRPIADNPAYTEAVLDRTRRCVERDKNHASVLMWSMGNESAYGCAFEAAM
                     AWTKERDDTRLCHYEGARYVPDVKKYDFSNMDLYSRMYPDQEEIIRYFEEQESRTFSE
                     NLPFMMCEYAHAMGNGPGDLEDYFELIHRYDGMCGGFIWEWCDHAVKTESGFLYGGDF
                     GEELHDGNFCVDGLVSPNRIPHPGLREFKNVYRPARIQSADWETGAIVFKNYMDFTDL
                     KDYVYLKWEAVQDGIIIEESIQKELPSVLPHTCGTVYYTGKLPENGSCFLRVRYLLKS
                     PVPGLKEGWELGFDEIRMPETAAGRQEKALGCESRPAACSGALTLEQTERRITVTGAD
                     FTYVYDTFSGAFSGIRKGEKEYLIRPMEYNIWRAPTDNDRKIRLEWERAGYDRTKVRT
                     YTTEARLREDGSAELVTELSLSAVHIQRILNIKASWLIHRDGCIQVKLDVKKDMEFPF
                     LPRFGLRLFLDQSLDRVRYCGMGPDESYPDKHQASWHGIFEGDVRTMHRPYLKPQEYG
                     SHWDCSWCEISGEEMSFQASSKEGFMFQACEYTQEELSEKAHDFELKEVGCTVLCLDH
                     NQSGIGSGSCGPQLLEKYQFNEPEFSRTWVITPGKRKE"
     gene            49979..51133
                     /locus_tag="ACZPCG_00935"
     CDS             49979..51133
                     /locus_tag="ACZPCG_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006773818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1283670.1"
                     /translation="MKRTKWIAFLCFAFLMAGLGANDALRGIFAPVFQERYDLNGSQL
                     SLIVTISYVGNLLFLSIGGKLLDLFPRKNVAVAVTAVWAGAVALNLVSDSYIWILVSM
                     FFALGASTLLNTTVNILTPAVFAGYAGLMVNVLFFIQGIGTSGSQLLLGRYAFHYAGW
                     KWINAALLTVAFLVIVLFFFLKIPKQGEEKEREKEILKKEPDSKIFWIFIAMMGCYFI
                     GEHGIMNWLMSYCISAFSMEASSASASLSLFWGGMTAGRLMFAPVVQKMGAVKSMKLF
                     GGIGTVMFCAGCILGESGVWILGASGFALAILYPTMVLLIQQIYPVQTAATRTGVIIS
                     AATVADIVFNLLFGAVSSKWGYGISFMILPVAMAVFYGLYLRLAGSLKRN"
     gene            51168..54386
                     /locus_tag="ACZPCG_00940"
     CDS             51168..54386
                     /locus_tag="ACZPCG_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HIR1283671.1"
                     /translation="MDVMRNRIRPILDSLRQWRCRFSEELTEVEIRPGDYHFSEKLDA
                     DQLKDGWMPFGACETWGGRDRHFWFRTKVVIRKEWAGKEVRVTLNTGADDIWNTDNPQ
                     IMAYKNGILSGTMDMNHQDLILAEEAEEAEVFELVFYAYSNSENPTNFFHLKAAVYDR
                     EIADLYYDLKVPFEAAELCSEEDLDRIEAMKFLNTAISGLDLRKPGSEEFYQSVREVR
                     EYVKKNLPDICRSSRVTVHSIGHTHIDVAWKWPLRQTRQKAVRSFQTVLNLMEQYPEY
                     KFMSSQPQLYEFVKEEAPELFEKIKEKIHEGRWEAEGGMWLEPDCNLASGESLIRHIL
                     YGKRFFEKELGAQKQEVLWLPDVFGYSAAMPQIMKKSGLSYFMTTKLGWNEYNVFPYD
                     TFMWKGIDGSQVLTHLITTRNYLPGCSLKDMPNGSTTYNGLQNPSQIKGTWQRYQNKE
                     TSCDVLTCYGYGDGGGGPTEEMLEQSRRMENGVVGVPCVRQTFVKDFFHILENKMDRN
                     CLPSWSGELYLEFHRGTYTSQAKNKKYNRICEFMMADAEFYSVLANGLGNGYEYPEKE
                     LEKNWKLLLLNQFHDILPGSSIKDVYEDSAVQYEEILRSAGRIAEGAGKAILSSLKEN
                     GNNKEGLAVFNPLSFRRTSAAELSAEEARWAENGLKGGCPEGMIIQRTGEGGMLILVK
                     DAPSKGLGVYENSVFDIQAEEPVIRSLVTDERGWPISLDTPFFHMEFDPQGEISCIRD
                     LRESRELLKKGAVGNELIVYEDRPMEFDAWNIDAGYREKKWKFGGVTEFYLEENGPVR
                     GSLLIRRHFLDSVLEQRICFYSHTARIDFKTKADWRESQLLLRTSFPLDIQSNEADFE
                     IQFGNVKRPVHQNTTWDQARFEVCAHKWMDLSEYGYGAAVLNDCKYGCDIHDSVMSLT
                     LIKSGIFPDPQADQGLHEFTYSLYPHRGDFRRGRVIQEAYDLNCPLTVQKQSGIKKGE
                     WSFLQISEENIFADTVKKAEEGDDLIIRLYEAYGMRTRVHLVFPLFSDFDAAVCDLME
                     QTDQKTQSFVSETELVQKGQTLEFEMKPYEIFSIRLTPKCCR"
     gene            54556..55878
                     /locus_tag="ACZPCG_00945"
     CDS             54556..55878
                     /locus_tag="ACZPCG_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006782267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1283672.1"
                     /translation="MKKKAARALAGVLTLSMAAALTACGGSDSAKKGGETAAAAESSG
                     PITGQTLSVMLSEEPGEQDALGNVLNHWAEESGNTIKKIIISHDDMRSKFPAMAKNKD
                     LPDIVSTTFLHQIYPDEFVNMEDVVDLSKFNQSALNIVGKSYSSDAITGITEQFTTTC
                     VFYNKDAFAAAGIEVPTPENPWTWEQLYENAAVLQEKGGVKYGFAADVSRARYDIMMY
                     ANGGSLVEKDGDSFRVTVNSPENIATLERFVQANNEVMPKAIWAGGTTDNPVEYFKNG
                     DAAILLSGSWNYNTFTNEISKFDFGVMPSPKGSECQSSIIGGAALAIPQNGKNSELAK
                     QFVQWLFEEEHYKEYLQLHKGLSVMNDVAYEPETDKAKEDFALMQGETAYVTDTFMTD
                     ESSSWRNYKDNEYRDALKRAVSGEITAKEALDGFAKQLSEASNWSMAY"
     gene            56113..57216
                     /gene="bglB"
                     /locus_tag="ACZPCG_00950"
     CDS             56113..57216
                     /gene="bglB"
                     /locus_tag="ACZPCG_00950"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002600880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA];
                     GO_process: GO:0009313 - oligosaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase BglB"
                     /protein_id="HIR1283673.1"
                     /translation="MKYTAADIDRYIDLYLNSFEKVLYEEDSQFMEGMKTRSLAGDDI
                     RRYQFWEWTQGVGLYGVWKLYKETKDERYMNILTSYYERQLKIGLPSKNVNTVTPLLA
                     MSFVAEETDNERYMEVCREWAAWIYADFPRTKEGGLQHITSDTLNEGELWDDTLFMTV
                     LFLANMGRILGEKKYQDEAEYQFLLHIKYLTDRKTGLWYHGWSFKEKSHFAAAFWGRG
                     NCWITAAIPEFLDLCPCSPSVEKFLKETWKRQVEALIQYQEPTGMWHTLIDDKTSYVE
                     SSATCGFAYGILKGAELGLAETDWVEAALKALEPIMEYTDETGVVNQVSYGTPMGRET
                     KDFYKEIELKPMPYGQALAMLFFMECRKHISDF"
     gene            57241..>57573
                     /locus_tag="ACZPCG_00955"
     CDS             57241..>57573
                     /locus_tag="ACZPCG_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006782266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1283674.1"
                     /translation="MKKSRRQSFCHTHSFWLFVLPSLALFAVFFLVPLVLSIWFSFTN
                     YDGWKTMDVIGLKNYTDILKDGDFYKTVGRTLLYTVCNLPFKIAIPLLLAALVTSRLV
                     KGKTLMRTL"
BASE COUNT        16022 a        11442 c        15612 g        14497 t
ORIGIN      
        1 atgggacagt cgtatatgat cgataaattt ccttttttgg gaaatgaaat cgtaaggctt
       61 attttttcca gtcttcagtc aattaccagt attattttta acaatattcc acttttgttt
      121 gctatgggtg tagctcaggg aatggcaaaa caggaaaagg gaattgcagt atttgcatct
      181 acggttggat atctggcatt aaacatagta atgaatgttt atttaaaagc cacaggaact
      241 ctggcagatc ctgcagttgc tgctcaggtg ggacagggta ctgtacttgg aattcagaca
      301 ttaaagattg aggctctggg aggcctgatt gccggtcttg tagcggcaaa agtggcagac
      361 cgtttttacc gtctggaact tcctctggca tttgcctttt ttggaggaaa gaaatccatt
      421 ccgattatta cgtttgtatg tatgatccct attggtctgg ttcttcctgt aatctggaat
      481 gcactgacaa attttttaat cagtatttca tttattttta cagcacctta tatcggaagc
      541 tctgtttatt atactttaaa ccgtgcgctg attccttttg gccttcatca tgttctggct
      601 tctctggtac gttttacaga ggcaggcggt acctatatgc ttaatggaca ggaatatgtt
      661 ggtattttaa atgcaaccaa tgagattctg ttcaacctgg gtcctacaag cgaatactgg
      721 acacagctga tgccaaagct caccagctat ctgggtggtg cacagatgct tacaactctg
      781 ttccgtgttc cggcgatcgg actggctatg taccatacat cctatctgaa gaaccgcaag
      841 attgcgaagg gcgtaatcct gacctgctgc ctgacagcat ttcttggaaa tattacagaa
      901 ccgcttgagt tttccttcct gtttatttca ccggcgttgt ttatccttta ttgcgtaatg
      961 tgtggaatcg gacttcttcc atatcagtta ttaaagatca gtattggtta catccgcgga
     1021 actatttttg actttggtat ttttggtctt ctgtatgaga atacacactg gattggtctg
     1081 gttgttttag gaatttttaa ctttattgta ttttattttg tatttaaatg gtttatctta
     1141 aagtttgaac tggaaacacc gggaagagag tcctttgagg tagaggagtc agcaagcatt
     1201 ctcttaaaag aaaagaactg gccggctatt gctgagattg taatcgatgg tctgggcgga
     1261 aaagaaaata ttttaaatgt ggaaaactgt atttcacgtc tgagagtcga cctttctgat
     1321 cccaagaagg tagatcaggt gaaattaaag gattccggct gtgcgggaat tttcttcccg
     1381 tcagagaagc acatccatgt tgtttttgga cctcatgtgg aatttgtgcg ccatgcagtg
     1441 gatgataagt taaaataata aggggtggag accatgagag ctttatatga ttcaaaaagt
     1501 aagttggatg acaggggaat taagaagctt ttgagtgagg aaaataaata ccgtacatgg
     1561 ctgaagattg agcaggctct ggcatattca caggcagaag agggctttat tccgaaagca
     1621 gcagcaaagg atataggaag tgtagctttt gcagatctgg atatggaaga aatgagccgg
     1681 attaaagaaa aggtgggaca tggatttgtg ccctttgtaa aagtgcttgt aagggcctgc
     1741 aggggagaag gaaaaaaata tgttcattac ggtgtgacta ctcagaatat tcagcagacc
     1801 tcccaactgt atacagcaaa ggaagtgaca gagatctata aaagctttct ggcagatata
     1861 ttagagcacc tggcagctct tgccagggaa aatgcccata cggttatggc gggaagaacc
     1921 catggaaaac atgctattcc tattacatat ggttataaag tatctgtctg gatcagtgag
     1981 ctattaaccc atctggagcg ctttgaagaa gcagagaagc ggatctttac ggtgatgatg
     2041 ggaggagctg taggaggctt caatgccaca ggacttgtgg gaagaagagt tcaggagcag
     2101 gtagccgaac ggttaaacat gtcttccatg gaggttccca gtcgtaatat gagtcagatg
     2161 aaggttgaat atctgatgaa tctgtgtctt ctgtgcaata catttcacaa gatggcagag
     2221 gaagtgtatt atacgggaat tgaagaattt tctgaggtgt gcgaaggttt tacgcctgga
     2281 acgattggga gttccaccat gcctcagaag atcaatccca agctggcaaa gggaatcata
     2341 gcgaattccc agaagctgta ttctcttccg gcaacgggac tttattcggc tgtgcggatg
     2401 tttgagggtg acagcagttc ctatatgctt tttgatggtc ttatggaaga agcgatggag
     2461 cttactgctg aggtactgat ccgtgctgag gagcttacaa gaactttgaa tgtcaataag
     2521 gagcgaatgc ttgaaaatgc caatattaat catggactgg ataacagcga gtatgtgatg
     2581 atgaaggcag cagaacgtct cgggaaagat aaagctcatg aacttcttta tgataaggct
     2641 atgaaggcag aactggaaaa caaggactat tttactgttt tgacagaaga tgaaacactt
     2701 tcatccatgt ttacaaaaga ggaattaaag cagttgattg acccggccag ctatacaggc
     2761 ttgtgcagtg ttctggctgt ggaaatggca gataaagctg aaaaggcagc tgcaatcata
     2821 aaagaagagc ataaaaatca ggactcttgg gaggaattga aatgagagaa tcacatatta
     2881 ttaccattgc aggagcaggg agcggacgtg taccggcgct ggtaggtact ttggtacagt
     2941 ataaggaaag atttccatta aagaagatta ttttttatga tatagatgaa gaacgtatgg
     3001 gaaagatgga agcttatgac cgtctggtgt taaaatgcta ctacccggag tgtgaggtag
     3061 tatttaccac agatgaagat gaagcttaca gggaaacaga ttttgtgttc tgtcagatgc
     3121 gtgtaggaaa aacccggatg cgctcctatg atgaaaagct tccgcttaag tatggtctgg
     3181 taggtcagga aacctgtggg ccgggcggat ttgcctatgg tatgcggtct cttggagcca
     3241 tgaagaaaat ggtggaaaag gttcgttcct attcaaaaga cacatggatt ttaaattata
     3301 cgaatcccgc tgccattgta gctttgggac tggatcagat gttcccggac gataagcgga
     3361 ttttaaacct ttgtgatcag ccattttcca tgatgaagag ctttgcgaag attctgggcg
     3421 ttccgcagga aagtctgagg gccagatatt tcggtctgaa tcatttcggc tggtttacag
     3481 aattaaaaga tacggagggg aaggatcatt ttgaagagct tcgtacctat ttaagagatc
     3541 atgattttaa accttttaat gcggagcagc gttccaaatc gtggctggat acttatatgc
     3601 gtgtaaacca gtatatgaaa tattttgacg agtatatacc aaccacctat atgcagtatt
     3661 atatgttccc tcaggagatt gctgctgaaa gtgatccaaa ttatacaaga gcagatgagg
     3721 cccgggattc cagagaaaag caggtatttg atgcgtgcgc tctggcagag ggacgggaaa
     3781 atatggatgg aatagagatg ctgaccaaca gtgtatttgg aaacctgatg gtagaggtgg
     3841 cagagtccat tgcatacgat cttaatagag aatttattgt tcttgtacga aatgaagaaa
     3901 ttattccgaa ttttgaaaaa aatgctatta ttgaagtggc aggaaccatt ggaaaggatg
     3961 gagccaaggg ttatccttat ggagagattc ctctgtttta caagggattg atggaagggc
     4021 agtatgctta tgagcagctg accgtggatg cattcaggga aaagaactat gagaaggctt
     4081 tgatggctct cacagtcaat cgtacggtgg tagatcccgg taaggcaaag gctgttttgg
     4141 atgacctgat ggaggttaac cgagaatact ggtatcttca gtaacaggag gaaagacaga
     4201 tggttctgta ttcaaaacac attattttgg aaagtgaaga atttgatggt tttttagagc
     4261 tggagcatgg gaagattcag ggaatccata aaagctggga aggttcttat gaagactaca
     4321 gtaactgtgt gattcttccg ggatttattg atatccatgt tcatggctgg gctaccggtt
     4381 ctttctggta tgaaaaaaca gaggagtcgt tgcggaaaat gtgccatact cttccattct
     4441 caggagttac atcttatctt ggaacaacgg gagcggacag tgttgaggag attaagagat
     4501 gtattcatgc agcagatcag gtatatgagg aaaatgaaga aggggcagaa cttcttggcg
     4561 ttcatctgga gggaccgttt attaatccca agtacagagg aatgcagaaa gaggaatgct
     4621 gtctgaatcc ggatatacag gtaatgaaag aactgtataa tacatttcgt cataaagaac
     4681 tctgccgaca catgactctg gcagttgaac tgcccggagc cagggaggtt ttggaatttt
     4741 gccgggaaca cagaatacag acagctattg gccatagtgc agctacattt caggaaatca
     4801 gggatataaa agatgtggga atcggcggtt ttacacatac gttcagcgga atgaaaggct
     4861 ttcatcacag agagctggga gtagcgggtg ctgcgctgta tttcgatgac atgttctgtg
     4921 aatttgcaaa acagacaggt ttgacagtat cccatgaggc ctttgatatt gcttttcgta
     4981 ttaagggaag tgagcgtatt atactgacaa cagactgtac aggactggca cagactcaga
     5041 ctgaatttga gcattatgtg agaaagctgc gttttgtgaa ggatggaaat aaggttcgta
     5101 tcgaacattt tgacggacga acggaatggc ttgatccgga ggactacagt gcagtgaaaa
     5161 atgtggaaat gggttatgct gagtctgtga aaaacatggc tgcccataca aaagcagact
     5221 ggtttgatat tatgcggatg acaagtttaa atccagctag atatatccat gtagatgata
     5281 gaaaaggaaa aattgctccc ggaatggatg cggatcttac tattatggat caggataaaa
     5341 atctgatctg cgtgtactgc agaggaaggg agatccggga agcatgaaat gtgcggtaag
     5401 tgattttgac cggactctct atgtgaacgg agcagtcagt gaaagaaata tagaggctgt
     5461 aaagcaatgg cagaggaagg gaaatctgtt ttttatcgcg acaggaagaa atctggcatc
     5521 tattaaagag caattgggat tttttgatct ggaaccggat gggctgattc tgaataatgg
     5581 agcagctatt atggataaag aggggaagct tctgctgtgc cgtatcatag agaaaaaaca
     5641 gctcttaaaa tactcaggtt tcttcatgaa ttaaatgagg atggaagcgg cgtttccatg
     5701 acagataaaa aaatcaatgt actgtcttct tctggaacaa cgactcagaa agcctgtgat
     5761 ggagtatgca ggattgatca gattgaaggc cttgaatcaa ttctccagat tcatcggcgg
     5821 aataaagatg aagcatatat ccggaggctt tgcaaggtaa ttaatgagag atttgaccag
     5881 gtaagtgcat ttgcgaatgt atggaatgca gatattgtag caaagggagt ggataaggca
     5941 gctgcagtgc actggatttt aaaccgcaga ccggatattg atgaagttcg ggtaatggga
     6001 gacagtgcca acgatgcagg aatgatacgt gaatttcacg gtgcagcgcc ggtatgggct
     6061 tccgcagaag tgcgtcagac ggcagcagga gtacttaatg atgcagcaga gctgcttgaa
     6121 gattcatgtc catcagcagt atgttttgag tttaggaaaa attaactacc cattcctccc
     6181 aaaatgagtt atactgttaa ggaacgaata aagggaaatg attcctgcag ggtgcgctgc
     6241 atccgttgga gcaggagatc aggaaaggga gcaatatggg atttttattt ggaaaaaaga
     6301 aatcggagtt tgtatcaccg gcaggaggaa cactgatgga tatcacagca gtggcagatc
     6361 cggcattttc ttccaagatg atgggagacg gctttgccat tgatctgaca gagggcgagg
     6421 tagttgcacc ctttgacgca gaggtaacgg cagcttttcc aaccggtcat gcatatggcc
     6481 tcagaagaaa ggacggggta gagtgcctga tccatatcgg tatggataca gtagagttaa
     6541 acggcgaggg ctttgacgta aaggtagagc agggcgccaa ggtaaagcag ggccagactc
     6601 tggtagtggt agatttagag gcagtaaagg caaagggcaa atctctggtg actccggtga
     6661 tcttcccaag cggagccgca tttactatga acaaaaagga tggaatcgtt gcaaaggctg
     6721 aggagaatat tctgaaattt tcataagaaa tccaaaacag ggcagagacg ttaaaacagt
     6781 gcgttctgcc ctgtttttct gccttgatct gtacaagcaa gtttcatgcc aaaaaagatt
     6841 agattaccac aaaaaaattt aggattccaa gccattgaaa aataaaatga aataaagtat
     6901 aatctttaca atttgggctg acgcagaaat acggtccctg gagatgctta acacatttcc
     6961 aatccccaac aaacacacat aagaaaggac aggtaccccc atatgaaagc ataccttata
     7021 ttggaagacg gcactgtgtt caccggcacc agcattggag cagagcgtga agtgatcagt
     7081 gagatcgtgt tcaacacttc tatgaccggt tatctggaag tgctcactga cccgtcttac
     7141 gcaggccagg cagtagttat gacctatcca cttattggaa actacggaat ctgccgtgaa
     7201 gacatggaat cagagagagc atggccggac gcttacattg tccgggaatt atccaggatc
     7261 ccaagtaact tccgcagcga agacaccatt cagaatttct taaaggagca ggatatccca
     7321 ggtatcagcg gcattgatac aagagcactt acaaagcttt tgagagaaaa aggaaccatg
     7381 aacggcatga tcacgactaa agagtataca gaccactctg aggttttaaa gcggatcaga
     7441 aattactccg tagaaggtgt tgttttaaag actaccacaa aggaaaaata cgttcttccc
     7501 ggaaccggaa aaaaagtggc tctcatggat tttggagcaa agaaaaatat tgcccgttcc
     7561 ttaaatgaga gaggctgtga ggtaacggtt tacccggcgg acacaccggc agaggaggtg
     7621 ctggcagcaa acccggatgg catcatgctt tccaacggcc ccggcgatcc aaaggaaaat
     7681 acggctatta ttaaggaagt aaagaaactt tatgactcca atgtgccgat ttttgccatc
     7741 tgtctgggac atcagctcat ggcccttgca aacggcatgg acacctataa gttaaaatac
     7801 ggccacagag gcggaaacca tccggtgaag gatttggaga caggacgagt ttacatttct
     7861 tcccagaatc acggctatgc ggttgataat gcaactattt cccctgaaat cgcagaacct
     7921 gcatttatca atgtcaatga cggaacaaac gaaggcttaa aatacacagg aaagaacatt
     7981 ttcaccgtgc agtaccatcc ggaggcctgc ccaggcccat tggattccgg atacctgttc
     8041 gaccgtttcc tgaaaatgat ggaggtaaat gactgatgcc aaagaatcct gagatcaaaa
     8101 aagttctggt gctgggctcc ggacctatcg taatcggcca ggcggctgaa tttgactacg
     8161 caggcacaca ggcctgccgc tctttaaaag aagagggcat tgaggttgtg ctcttaaact
     8221 ccaatcccgc gactattatg acagataagg atattgcaga tcgggtatat attgaacccc
     8281 tgactgtaga ggtggtagag cagttaatct taaaggagca gccggacagt attcttccta
     8341 ctctgggtgg ccaggcaggc ttaaatcttg ccatggagct ggaagacgca ggcttcctta
     8401 gagagcacaa tgtgcgcctg atcggaacta cagccctgac catcaaaaag gccgaggacc
     8461 gtgagatgtt taaggaaacc atggaaaaga tcggtgagcc ggtggctcct tctgatatcg
     8521 tggaggatgt aaagacaggt cttgagatcg cagcaaagat cggctatccg gtagtgcttc
     8581 gtccggctta taccctgggc ggatccggcg gcggtattgc ccacaatccg gagcaatgtg
     8641 cggaaatcct tgaaaacggc ctccgccttt cccgcgtggg acaggtgctg gtagagcgct
     8701 gcatcgcagg ctggaaggaa atcgagtatg aggttatgcg cgacggagcc ggaaatgtga
     8761 ttactgtctg caacatggaa aacattgacc cggtaggcgt acacaccgga gacagtatcg
     8821 ttgtggctcc ttcccagaca ctgggagata aggagtacca gatgcttcgt acctctgctt
     8881 taaacatcat cagcgagctg gggatcaccg gcggctgtaa tgtacagtat gccttaaatc
     8941 ccgacagctt tgaatactgc gtcatcgagg taaatccccg tgtgagccgt tcttctgccc
     9001 tggcttccaa ggcgaccgga tatcccatcg caaaggtggc tgcaaagata gctctgggtt
     9061 atactctgga tgagatcaaa aatgccgtta caaagaaaac ctacgcaagc tttgaaccta
     9121 tgctggacta ctgcgtagta aagatgccga gacttccatt tgataagttt atcagcgcta
     9181 agcgtgctct gggaacccag atgaaggcaa cgggcgaggt tatgagtatc tgcaccaact
     9241 ttgagggcgc cctgatgaag gccatccgct ccctggagca gcatgtagac tgccttctgt
     9301 cctatgattt tacagagctt tcctatacgg agctggtaaa tgacctctat aaagtggatg
     9361 atatgcggat ctggcgaatt gccgaggcgc tgcgccgtgg cttcccatac ggcctgatcc
     9421 atgagatcac ccgcattgac ttctggttta ttgacaagct ggcgatcctg gtagagatgg
     9481 agaaggagct tcaggcagta ggagaaagaa agaaggagct gactcctgag ctgcttgctg
     9541 aggcaaagcg cattgagtat cctgacaatg tgattgccag actaacaaag atggagcaga
     9601 gcgctgtcaa gaagctgcgc tatgacaata atatccgtgc tgcctataag atggtagata
     9661 cctgtgccgc agagtttgcc gcagaaactc catactacta ttcctgcttt ggaagcttca
     9721 atgaggcaga gagaacagag ggcagaaaaa aggttctcgt tttaggctcc ggtcccatcc
     9781 gtatcggaca gggaatcgag ttcgacttct gttccgtaca cagcacctgg gcattcagca
     9841 aagaaggcta tgagaccatc attatcaaca ataaccctga aaccgtaagt acggactttg
     9901 atattgcgga taagctgtat tttgagcctc tgacaccgga agatgtggag agcattgttg
     9961 atattgaaaa gccagacgga gcagtggttc agtttggcgg acagacagcc atcaagctca
    10021 cagaagccct gatgaaaatg ggcgttccga tccttggaac agcggctgag gatgtagacg
    10081 cggctgagga tagagaacgc tttgacgcca tcctggagca gtgcggtatc ccgagacctg
    10141 ctggccatac ggtatttact gccgaggagg caaaagaggc tgctcataag ctggggtatc
    10201 cagttctggt ccgtccctcc tatgtgcttg gcggacaggg aatgcagatt gccatcaatg
    10261 atgaggatat tgatgaattt atcggcatca tcaaccagat tgctcaggag catccgatcc
    10321 tggtagacaa atatatcatg ggtaaggaga ttgaggtaga cgctatctgt gacggtacag
    10381 acatcctgat ccccggcatc atgcagcata tcgagcgtac cggtatccat tccggcgaca
    10441 gtatctctgt ttacccggca caggatatta cagagaaaaa tgtggaaacc attgtggact
    10501 acacagccaa gctggcaaga gcccttcatg taaagggaat gatcaatatc cagtttattg
    10561 tagacggcga ggatgtatat atcatcgagg taaatccacg ttcctcccgt acggtgcctt
    10621 atatcagtaa ggtaacaggt atccccattg ttcctctggc aacccagatc atctgcggac
    10681 ataccattag agaactgggc tatacgccgg gcctgcagcc tgcagctgat tatatcgcca
    10741 tcaagatgcc tgtattctcc tttgagaaga tccggggcgc agatatcagc ctgggacctg
    10801 agatgaagtc caccggagag tgcctcggta ttgcaaagac ctttaacgag gctctgtata
    10861 aggcatttga gggtgcagga atccgcctgc ctaagtataa gaagatgatc atgactatcc
    10921 gtgagagcga gcaggaagag gctgtagaga ttgcacgccg ctttattgca gtgggatatc
    10981 agattttcgc taccagaggc accgcccgca ccttaaatgc ccacggagta aaggcatatg
    11041 agatccgcaa gctggagcag gaatctccga acatcctgga tcttgtactg ggccatcaga
    11101 tcgacctgat cgtggatatc ccggctcagg gagcagagcg cagccatgac ggctttatca
    11161 tccgccgcaa tgccattgag acaggcgtaa atgtcctgac tgccctggat acggcaagag
    11221 cacttgtaac cagtctggag aaccgggcca aggatctgac actgattgat attgcaacag
    11281 tgaagaacgc ttaaaatttt taaaggatgc tgcagaaaca gaacatataa gttttgtttt
    11341 gcagcatcct tttgttgtat ggtaaaattc taaagcagaa aataaaggga gccctatggg
    11401 ctccctcaga attgacagta tatccagtta tccgtttata gtcgttcctg cctcgccctt
    11461 caaagcctct tcggccttgt caagagaggt gataattgcc ttgcgtccct ttccgcactc
    11521 taaaaagtct acggatgcgg agatttttgg aagcatggag gcaaattcaa actgtccctg
    11581 gctgatgtaa tccttcgcct ggtcgatggt catgtttttg atgggctctt catggtctgt
    11641 accgtaattt aaggtaacat tgtccacgct ggtaaggatc atcattacat cggcatcggt
    11701 ttcctttgcc agaaggcctg ttgccagatc tttttcaatg atcgcgctgg caccccggag
    11761 attgtacccc tgttccataa ccggaatacc gccgccgccg caggcgatga cgatctgatc
    11821 agcatctaaa agagccttga tggcgtcgat ctctacaatg gctacaggat ggggagatgc
    11881 cacgatacgg cggaagcctc cttccacctc cttcacatga tttccctttg cctcttcctc
    11941 atctgcctct tcacggttca tcactcttcc gatcaccttt acaggagtat agaaggcttc
    12001 atcataagga tctaccgtta cctgagttaa aacagtgctt acaggcttat agatgccccg
    12061 gcggatcagc tcggcgcgga tgccgttctg cagatcatag ccgatatagc cctggctcat
    12121 ggcggagcag acagacatgg gggcagaact gtagccgtca tgctggcgtc cgaactcgtt
    12181 cattgcagtg tggatcatgc cgacctgcgg ggcgttgctg tgcacgatag cgacctgcca
    12241 gccctcctgg ataaagtcgg cgatcacctt tgcagtgcgc tgtactgctt ccttctgttc
    12301 cggaagatta gttcccatat ccttgtggcc aatagccatt acaatgcgtt tttttgccat
    12361 attcagtccc tctctttgta tgcggtgttg ttatatctat tttaatttat tatctctgaa
    12421 aatgcaacga aaaaagatac tggaaaaaga aatgcacccg tgatatactg gcatcatgca
    12481 gcaaaataca gacatactga ggaatacaca gatggtggaa aagaaatcat ttctccctcc
    12541 ggggtggcag aggctgctcc ttgggacggt gcttgctgct ttggcaggaa atatactggt
    12601 acgcctgatc ccctggcctg agagctggct gatttcttat ggggaagtac agacagcaga
    12661 gcgtatgagc cagtcgtatt atctggcgct taccctggcg gcggcaccgg tgatggagga
    12721 ggcggtattc cggctgggca tttatggact tctaagaaga aagcttaggc cgaaagcagc
    12781 agcttttctt tcagctttgg cctttggctt ttaccacgga aacatgattc agggccttta
    12841 cgctttttta ctgggctttg ttttggcatg gggatatgag aaatgtccct ggggaaaata
    12901 caggatggcg gtgctgatgc acgcggctgc caatggggca gccctgctgg ccttcggcta
    12961 aagctgcctg ccgtccttat ctgcatacat gcaaggagga aaaaacgtga aattactttc
    13021 agtagcgata ccctgttata attcagaagc ttatatggag cactgcatcc gttctcttct
    13081 cactggaggg gatgaggtgg agattctgat cgtagatgac ggttccacaa aggaccggac
    13141 ggctgagatc gcagatgaat atgaaaagcg ctatcccggt atctgccgcg cgatccatca
    13201 ggaaaatggc ggtcatggcg aagctgtcaa tgcgggattg agaaatgcaa cgggcgtatt
    13261 tttcaaagtc gtagacagcg acgactgggt aaatgaggaa gcatatcaga aggttctgga
    13321 aaccctgcgc cggttcgttt accgggggga agctctggat atgttggtga ccaatttcgt
    13381 atacgaaaaa gagggagcaa gacgtaaaaa ggtgatgaaa taccataccg cctttccaaa
    13441 ggactgcgtc tttggatgga aggatgtaaa atttttcatg atcggtcagt acatactcat
    13501 gcattccgta atttaccgga cacagctttt aaaggaatgc gggctggagc ttcccaagca
    13561 taccttttat gtagacaata ttttcgtcta ccagcccctt ccccatgtaa aaaatatgta
    13621 ctatctggat gtgaattttt accgctattt tatcggaaga acggatcagt ctgtcaatga
    13681 atccgtgatg atcggccgca ttgaccagca gctcagggtt acgaggctca tgctcggtta
    13741 ctatgatgcc actaaaatat ccaatccaaa gctgcgccat tacatgatcc agtatctgga
    13801 aattatgatg actgtctgct ccgttttggc gatcaagtca gggactgcgg agaatctggc
    13861 taaaaagaag gagttgtgga atgagctcaa aaagcagaat tttcctctgt ggctgcgtct
    13921 gcgctgggga tttctgggac agggctgtaa cctgccggga aagggcggaa gagagctgct
    13981 gatcctggga tacaagatca cacagaagtt ttatggattt aactaactgc tcaatctagg
    14041 caatataaag taaaagagcc gccggggaag cgaaatgata tgctttcccg gcggcttatt
    14101 tggtttattt gcagccatga tccttattct aaaatttttt ccacaatctt tttttcttct
    14161 tccacagggc tgcaggcata aacaagatga tacatcatag cagccataat tcctgcgccg
    14221 actccgtcga tcacggaatg ctgctttaaa aatacagtag aaagacagat cgctgcggtt
    14281 aaaatggctg cgcctgcctt taaggcagtt tttccctgaa acagagggct tttatggatg
    14341 gcaatatagg taccgatgga attgtataca tggatgctcg gaaatacgtt tgtacaggta
    14401 tcggtacggt acagagcagc cacggcagca gtaaaaatat ttttatcggg gtcaagaatc
    14461 ggacgcagat tggtaccatt ttcaaaaaag gtacagatga tcaggctgat ggtcatgcct
    14521 gaaaacagaa aggtgcacat ccggtaataa tcctctttgc ttttaataag gaagtaaatc
    14581 agcgtcactc ctacaaaagc aaaccagaga agatagggaa tgataaaata ttcattaaaa
    14641 ggaatcagac tatccagagg ggcgtgcatg agatgataat cgcgtgtcac tgttttttcc
    14701 agataacaga accagggaat atagataaaa aaataactta aaagccatat atggctgtat
    14761 ttgcggaaga aatcacaaac tcgtttcata gaactgtttc cctttctaag agtttaaaac
    14821 gaatgtaaag gaactgttaa aatttgtaga tgtgcaaaat tatagcacgg taaaaatgga
    14881 aaaacaagag ctggacagaa atcttaataa agtttcaaga gctttaagaa gttgccctgc
    14941 aaactgcgtc ttttcatttt atgtatcttg tgttatcatt attatatgaa ataaatttac
    15001 atgtaaatga aaggacatgg catgagtgaa gcacgaagcg tattaggcgt gatctgttct
    15061 ttctatgaca gcttttttga ggcggaaaag aagattgcgg attatatcat ggagcacaag
    15121 gctcaggcag tagatatgac ggtgggggag ctggcaagag ccagcggcac aagcgacgct
    15181 acggtatcca gattttgtcg cagatgcggg tttaagggat tccagagcct aaagctggct
    15241 ttggccagag aggtgctcag ggaagaacag gacagccgga aggtgacaaa tgatattgac
    15301 cgggagaatc tgtcccagtc acttaacaat attctggcaa ataaggttgc ggagctgaca
    15361 gaaacggtcc gtatgatgga tatacacagc ctggaagaaa tccttaaaaa gctggaaaag
    15421 gcccgtatgg ttcagctggc tgcagtggga aatacgatcc ctgtggctct ggacggcgct
    15481 tttaagttta atcagctggg gattccgtct gtggccggag acatctggga aacgcaggca
    15541 gcttatacct ttaatctggg gccggaggat gtggtgctcc ttatttccaa ttccggtttt
    15601 tccaggcggt taaacacact ggctcaggga gcaaaagaaa atgggagcac gctggtgctg
    15661 atcacgaata acccggattc gcctctggct tcgatctgtg atcataaaat tattacggct
    15721 accagagaaa agcttcttac agaagaattc tggttttcac gggtcacagc cacggcggtg
    15781 attgaaattt tatatcttct tttgcgggcg ggaaagaagg atgctatgga acatatccgc
    15841 cgccatgagg aagctatttc ctcggataaa aacgaaatat aggtaaaatg tacaaaaaag
    15901 agacggtatc tgacaggcag agaccgtctt tttgtttgaa gtgatgaaaa tataagagaa
    15961 acaaaatttc ataagaaata atattgacga aaaacaaata tcgtactata atataggtgt
    16021 gaaattaaat ttcttataaa taaggggaga tgacatatga tttacacagt tacctttaat
    16081 ccggctctgg actatgtgat cgctgtggat cattttgctc agggaaaggt caaccgtaca
    16141 gtaaaggaag atattttctg tggaggaaaa ggaatcaatg tttctgtgct gctggcaaat
    16201 ctgggagttg agagcacagc gctggggttt gtggccggat ttacgggaaa agaaattgaa
    16261 agggggttaa aagcatttgg gatctcatcc gattttatcc atgtaaaaag tggaatgtca
    16321 cgtatcaatg tgaaactgaa atcggaggaa gaaacggaga ttaacgggat gggcccggat
    16381 atccggcctg aggatgtgga agcgctgttt gaaaagctga accgtttagg gagcggggat
    16441 gtgctggtgc tttccggaag cataccggct gctgtgggaa atgatattta ccgtaggatc
    16501 atggaacagt taaaggatcg gggcgtgtgc attgctgtag acgcagaacg ggatctgctg
    16561 acagaggtac ttccattaaa gccttttttg atcaaaccta ataaccatga gctggggcag
    16621 atctttggaa aggaactaaa gacggagaag gagatcatcg atggcgccat gagcctaaaa
    16681 gaccggggag cggtcaatgt gctggtgtcc atggcaaagg acggagcaat cctggtcgat
    16741 gaaaatggaa aagtccacag actgggagtg tgccgtggaa cggtgaaaaa ttccgtagga
    16801 gcaggcgatt ccatggtggc gggatttctg gcaggatatt taaaaacagg agattacgat
    16861 tatgcgctgc gtctgggaac ggccagcgga ggagcgacgg cattttcaga tggtatggga
    16921 agcagaaaaa tgatcatgga tctgtttgca cagcttacag aaaatggagg gaagtagagg
    16981 atgcggatca cagaattgtt gaaaaagcaa agcattgaac tgggagtgaa ggcagcttcc
    17041 aaggaggagg ctattgatcg tctcatcgcc cttatggatg caggggggag gctgaatgac
    17101 agagccggtt ataaggaagg gattcttgca agagaaagcc tggggagcac agcaatcggg
    17161 gaagggattg ccattcccca cgcaaaggta gaagcagtaa aggaaccggg gctggcagcc
    17221 ataacagtgc ctgagggtgt ggattatgag gcgtttgacg gctctctgtc caatttgatt
    17281 tttatgatcg cagctccggc aggaggagaa gacgtacatc tggaggcatt atcccggctt
    17341 tccaccctgc tgatgaatcc tggttttaag gaagctctga tccatgcagg atcaaaggag
    17401 gagttcctgg aggttatcga tcaggcggaa acggagcggt ttgggaagaa agaagaaatg
    17461 gatgaaccgg aaaaggcagc agaaccggaa aaaacaggct ttcgcgtgct ggcagtcact
    17521 gcttgtccca caggaattgc ccatacctat atggctgctg aaaatctgga aaatacaggg
    17581 aaaaaactgg ggatttccct gaaagcagaa acggatggtt ccggcggcgc tcagaatatt
    17641 cttacgaaag aggaaatcag ggaggcagaa gcaatcattg ttgcggcaga taagaatgtg
    17701 gaaatggcgc gttttgacgg aaagcccgtt atcatggttc cggtggcaga cggcatccac
    17761 aaggcagaag agctgatccg gagagcagtc agcggtacag tgccggtata tcaccatgag
    17821 ggcggaacct ccgaggcaga agcggaagga gataaaagta ttggaagaac catttacaag
    17881 catctgatga acggcgtatc ccatatgctt ccctttgtga tcggtggcgg catcctcatt
    17941 gcgttagcgt ttctgcttga cgattattcc attgatccgt ccaattttgg aaaaaatacc
    18001 cctctggctg catatttaaa gaccatcggc gaacaggcct ttggaatgat gctcccgatc
    18061 cttgcaggct tcattgccat gagtatcgca gaccgtccgg gacttgcagt aggaatggta
    18121 gctggtatgg tggcgaaaat gggctgtacc tttgcaaatc ctgcgggtgg tgatgtaaat
    18181 gcaggctttc tcggagctct ctttgcaggc tttgcgggcg gttatctggt gttggggctt
    18241 aaaaagattt tttccagact gcccaaatcc ttagaaggta tcaagccggt gcttctttat
    18301 ccggtgcttg ggatttttgt ggctgctgta atgactacac tgatcaaccc atatatgggt
    18361 atgatcaatg atggactgac acatttctta aatggaatgg gcggtgccag ccgtattgtg
    18421 cttggaatgg tactgggggc tatgatgtct attgatatgg gaggtccctt taataaagcg
    18481 gcctatgtat ttggtactgc ccagctggca gaggggaatt ttgaggtaat ggctgcagtg
    18541 atggccggtg gtatggttcc gccgatcgcg attgccctgt gcactacatt ttttaagaag
    18601 aagtttacag aaaaagaacg ccagtccggc attgtaaatt atattatggg actatccttt
    18661 atcacagagg gagcgattcc ctttgcagca caggatcccc ttcgcgtgat tcctgcctgt
    18721 gggatcggag cggcggctgc aggaggcctt tccatggcat tcggctgtac cttaagggcg
    18781 cctcacggcg gcatcttcgt actccctacc attggaaatc ctcttatgta tctggctgct
    18841 gtgatgggag gagctgtcgc aggatgcctg attcttgggc ttcttaagaa aaatacagaa
    18901 gaataacaga tagaaacaag cccggctgat caggtatggc cgggcttttg ctgtgcaaaa
    18961 attcagttag atgcggtcgg cgctgtcgcg catatacttg tactctgcca cagaacggtc
    19021 aaagcctttg atgtggtagt agagccagtc tcttacattt tctgccacct tggaaacaaa
    19081 ggcgtcagaa gggccttctt cgtctgtaag catatcgtga agctcctgta cagtacggcg
    19141 cagctcctca tgcttttcaa ggtgctcctt caggcccgga tatccgattt ccttctggaa
    19201 tgcctcttcc tctttgaaat ggaaatccgt atagtctgcc agataattta ataatctggc
    19261 agctccgctc tggttggaac ggttctcaca gctgactaag aggtcattga tccggctgat
    19321 cagctctctg tgctggctgt cgatcatttc atttcctgtg atcaggtcat ctgtaaattc
    19381 tgcatacatg gcttaaatcc tcctgctttc atggtatagc aaaagtatac cacggggagg
    19441 cgggaaagtc tacgaatgat ttgaggtact taaaggtact caaaccaaaa acaaagaaac
    19501 ccttgattct tcaagggttt cagagtagcg gaagggagat tcgaactctc gacaccacgg
    19561 gtatgaaccg tgtgctctag ccaactgagc tattccgcca tatttaattg aaatggatgg
    19621 ggcctacagg gctcgaacct gtgaccctct gcttgtaagg cagatgctct cccagctgag
    19681 ctaagacccc gtatccagtg tgtcgcttgt ttgtttttgt ctcactcgcg actgcttgta
    19741 cagtatatga taaattgtgg taattgtcaa cacttttttg aatttttttt aaaaaagttt
    19801 ttttgccctt atttttgctg gttttccggc attttcagag atgggagagc atttattttt
    19861 tcgatatttt atagaaaatt ttttaatggt ttatattcat ttgggggcgg gcgtggtata
    19921 cttattccag agtagatgct gccatcggcg gacgggcgca ggcttgtgct ttccgctgtg
    19981 tggacgaaag gaaaggaccc tacgccccta tgtactattt tattgtaaat cccagtgcag
    20041 gctccggcag aggcaggaga gtgtggaaag ccattgcctg ctatatggat ctgcacaata
    20101 ttgaatatga agcggtactt accggcagcg tcggtgatgc gaggattgct gccagagaac
    20161 tgacggagaa gaaacgccag ccaggcctgt tgatcgtggt cggcggaagc ggaacggtca
    20221 atgaggttct tgatggagct gtccttcatg ggatttttga tctgggttat attccggcag
    20281 gcacgggaaa tgatttatcc cgctgtctcc acattcccaa acgtaccgta cgctgtctga
    20341 aaaaacagct gtatcccaga catttttcaa tggtagatta cggggtgctc tctcttggtt
    20401 ccagtgagat ttctcatcgg agatttatgg taagcgccgg tatgggattt gatgctgctg
    20461 tgtgttgtgc ggcatccgct tccaggctgc ggggcattat ggggcggctg ggtcttggaa
    20521 aacttttttg ttttcttctt ggactctgcc aggcggcagg atgccggtcc agccggggat
    20581 atatcctttt ggatggtgta aaaaaagtgg aatttaatca tatcctgttt atatcctgtc
    20641 acattcagcc atcagaggga ggcggttttg tttttgctcc cagagcagac ggaagcgacg
    20701 gcaggatgaa tgtctgtgtg gtaagccatt cttccagaaa acggctgatc cctgtgatgc
    20761 tgcaggcttt gaccggccgt tttaagaagc ataagggagc acggatgttt gaatgcagag
    20821 aggtttccat tcacacagac gcccctcttc ccattcatgt ggatggagaa ttctgcggta
    20881 ctcagacaga tctgcgggca gagtgcattt cgcggcagat ccgaatgggg atataatacg
    20941 gctgttccgg ccggtaatat gaactgttaa aaagaattgt gatcaaaaga acgaaataga
    21001 ggagacagaa acatgagaat cggacaaggc tatgatgttc acaggctcac agagggccgt
    21061 aagcttattc tgggaggcgt ggagatacct tatgaaaagg ggcttttagg ccattcggat
    21121 gcagatgtgc tgatccatgc cgtgatggat gcgctcttag gggcagcggc tctgggagat
    21181 atcggacagc attttccgga tacagatcct gcttataagg ggatttccag catagagctt
    21241 ctgggtaagg taggtgcact tttggaggaa aagggctatg tgattgaaaa tattgatgct
    21301 acgatcatcg ctcagaggcc aaagctggcc tcctaccgtc ctcagatgga agagaatatt
    21361 gcagaggccc ttcatctgga tccgtccaga gtcagcgtaa aagccactac agaggagggg
    21421 ctgggattta cgggaagcgg agagggaatt gcttctcagg caattacatt attgacggaa
    21481 acagcggatt actgctatga taataaaata atgacagaag gctgtccggg ctgctcaggc
    21541 tgcggctgcg agcagaagtg aacagtgttt ctgaatgatc ataagcgagg agaaatcgta
    21601 tgaaaatatt taatacatta accaggagaa aagaagaatt taagccgatt acaccgggag
    21661 aggtgaaaat gtatgtgtgc ggccctacgg tatacaatct gatccatatc ggaaatgccc
    21721 gtcccatgat catgtttgac actgtgcgcc ggtattttga atacaaggga tataaggtga
    21781 aatttgtttc caactttacg gatgtggacg acaaaatcat caaaaaggcc aatgaagaag
    21841 gcgtagatgc ccatattatt tccgagcgct atattgcaga gtgcaaaaag gatatgaagg
    21901 atatgaatgt ggagcctgct acggtacatc cgcaggcaac tcaggagatt cagggaatga
    21961 tcaatatgat ccagaccctg atcgacaagg gacacgccta tgtggcagag gacggcacgg
    22021 tgtactttaa gacgcgttcc tttgcagaat acggcaagct gtcccacaaa aacctagatg
    22081 agcttcagtc tggctttcgt gagattaagg taacgggtga agaaggcaag gaagacccta
    22141 atgattttgt gctctggaag cccaagaaag agggagagcc ttactgggag tctccatggt
    22201 gccagggccg tccgggctgg catattgagt gctctgtgat ggcaaagcgc tatctgggag
    22261 attccattga tatccatgca gggggcgagg atctgatctt ccctcaccat gaaaatgaaa
    22321 ttgcacagag tgagtgcgcc aatgataagc cctttgccgc atactggatg cacaacggct
    22381 ttttaaatat tgataataag aagatgtcca aatcccttgg gaatttcttc actgtccggg
    22441 atattgcgga gaaatatgat ctgcaggtac tgcgtttctt catgctcagc gcccattacc
    22501 gcagtcctct taatttcagt gccgagctga tggaggcttc caaaaacggc ctggaacgca
    22561 ttatgacctg catggataag ctgcgggaac tgaaggcaaa ggctcaggcc ggtgatatga
    22621 cagcagccga gacagagaaa atggcagagg ctgacaggct cagagagaaa tttgaggctg
    22681 ccatggagga tgattttaat acggcggatg cggtttccgc tgtgtttgag ctggtaaagc
    22741 ttgctaactc tacagcagat gctgattcca ccgcagctta tgtaaagggc cttgaagatc
    22801 tgatttcacg tctgtgcgag gtgcttggta ttatcacaga gcggaagaca gaggtgctgg
    22861 acgaggagat cgaagccatg atcgcagcca gacagcaggc cagaaaagag aaaaactttg
    22921 ctcttgccga tgagatccgc ggaaagcttc tggatatggg aatcgtgctg gaggatacca
    22981 gagagggcgt aaagtggaag agagcataac attaaaggac ttccttgcat actataaaaa
    23041 gaccatgaga ctggaaccgg tagatgccgg ttcctattct ccgttggttc ttgcttacat
    23101 tggagatgcg gtgtatgagg tgatgatccg cgccagagta attaaccgtg gaagtatgca
    23161 ggtaaataag atgcataaac gaagcgcgat gctggtaaac gcaggcacgc aggcacagct
    23221 tatccgctcc attgaagagg agcttacgcc tcaggagcac gcggtctata aacgggggcg
    23281 caatgccaaa tccgttacca tggcaaaaca cgctactatg gcagattacc gtactgctac
    23341 aggcatggag gctttggtgg gctggctgtt tctggaagag agatttgagc gtcttgcaga
    23401 gttgatcagc ctggggcttg aaaaaatagg agcctttgag gaaatataaa ggagaaacat
    23461 ttcatgagat acgaagaact gaccatcgag ggacgcaatg cagtgctgga ggcctttcgt
    23521 tccggaaaga ccattgacaa gctgtttgtg ctggacggct gccaggacgg cccggtgcgc
    23581 accatcgtaa gggaagcgaa aaaacacgac acaattatca actatgtggc taaggagcgt
    23641 ctggatcagc tttctgagac gggaaagcat cagggagtga ttgcttatgc ggctgcctat
    23701 gaatacgccc aggtggagga tatgcttaag ctggcagagg aaaaaggaga gccgcccttt
    23761 ttgtttttgc tggatggcat tgaggatccc cataatttgg gagcgattat ccgtacagcc
    23821 aatctggctg gtgctcacgg cgtcattatc ccaaagcgca gggcagtggg gctgactgcc
    23881 acggtggcca agacttcagc cggtgcgctt aattataccc ctgtggcaaa ggtgacgaac
    23941 cttaccgcag tgatggagga tcttaagaaa aaggggctgt ggtttgtctg cgccgatatg
    24001 ggcggggacg tgatgtaccg catgaatctt acaggcccca tcggccttgt gatcggaaat
    24061 gaaggcgagg gcgtcagcaa gctggttcgg gagcactgtg atatgacagc ttccattccc
    24121 atgaagggag atattgattc gttaaatgcc tctgtggcag caggtgttct ggcctatgag
    24181 attgtccgcc agaggctggc aaaatagcag tttggaaaga ggaagaaaga atggagagaa
    24241 tccgaaaaaa cagaagaaac atttccgggg cagcggttct gacagccgct gtggtaggtg
    24301 tctgtggcct ttttggagca tttccggcaa aagcgtctca gacagcagtg atcgaggata
    24361 cacaggtgcc gctgtatgat aagcctgccg gaagctatgt gcgtactccc attgcttccg
    24421 gtgtgacggt atataaaaat gataaggcca ctctggatgc gtccaataca tctgagggct
    24481 atctaatggt gaaatatacc ggcagtgtag gaaaaattaa ggttcagatt tccaaaaacg
    24541 gttcagaaac atatacatat gatttaaata acagcggtgt ttatgaagtt tttcctcttt
    24601 cggagggaaa cggctcctat caggtaaagg tgtttgaaaa tattcagggg aatcagtatt
    24661 cccaggcctt cagccagaat ctggacgtat ccatttccaa tcccttcggt ccttttttgt
    24721 atcccaacca gtatgtgaat tttaattctg ccagtgcggc agttcaaaag ggagcagagc
    24781 tttcggcagg cgttacagat cagcttggcg tggtatcggc tgtttacaac tatgtggtta
    24841 agaatctgac ttatgatacg gcaaaggcca gttccgtaca gtccggctat cttcccaatg
    24901 tggatctggt gctggcccag aaaaaaggaa tctgctttga ctatgcggct cttatgacgg
    24961 ctatgctccg ttctcaggac attcccacca agctggtggt gggatataca ggaaacctgt
    25021 atcacgcctg gatcaatgta tatctggaag gccagggctg ggtggacaat atcatttatt
    25081 ttgacggaac cagctggaaa ttgatggatc cgacctttgc atcctccggc aaccagagcc
    25141 aggagattat gcagtatata ggaaacggag ccaattacca ggcgaaatac agctattagt
    25201 caggaggaca gagaaaatgg aaaaacaggg ggagaaatac gatcggtatt cctatgagga
    25261 gctgtccgcc ttctgcttac agattgccct tttgctggag gcggctgtgc ctttggagga
    25321 agggctgacg atcatggcag aggatgcggc agaggaaaaa gaaaaggcca tgcttcttta
    25381 tatggcagag ggcgcagaac ttggagatcc tttctttaag gtgctggagg atacaggcgt
    25441 gtttcccccc tatatcgtcc gtatggccag actggggcag cagacaggta cgctggatca
    25501 gatgatgaaa tccctttctg attattatga gaaagaagcc aggcttttaa aggcggtaaa
    25561 aaatgcggcc acttatcccg caatgatgat tctcatgctg ttagtggttc tattcgtgct
    25621 ttttacaaag gtaatgccgg tgttttcaag ggtatatgag cagctgggag ccagccttcc
    25681 gcctgcggct gcagcagcaa tccgtctggg aggtatttta agcggcgcag cattggttct
    25741 ggcagctgta actggtgcag ctgcggccgt gatatggatt gctgcccgtt tcggaaagcg
    25801 tttttcactg gtggaaaagg gcgtcgcagc agtaaaaaac cgcagccgta ttgccagagc
    25861 agttgcgctg cgccgcttta cctctgtgct ggctctgacc ttaaagagcg ggctggaact
    25921 ggaaaagagt atggaattag ccaaagagct ggctgaaaat gaaacagtgg ccggtcagat
    25981 cgatgactgc tccagacgtc tggaagaggg agaaagctac tatgacgcca tgaaggcagc
    26041 agggcttttt tccggttttt atattcagat gattaaggtg ggaagccgca gcggccatct
    26101 ggatcaggta atggaggaaa tttccgggga ttatgaggaa atggcggatt ctgccattga
    26161 cggaatgatc gcccggtttg agcctacgat cgtagctgtg ctggctgtat cagtgggact
    26221 ggtgctgctt tccgtgatgc tgcctctggt aggcgtgctt tctgcaatcg gttaggagga
    26281 gctggatatg agagtaaaac gcgggcagga ccgaagagga cggggccttg cgggcatgat
    26341 cggctcgatg ctccttatga tggcagcaat cctgtttttt accctgttct ctgtacagtt
    26401 tggagagaga accacagcca gagatcagga aaccctgaaa aaggccatag caagagcaag
    26461 tgtacagtgc tatgccattg aggggagata tccgccaagt atcgagtatc tggaggagca
    26521 ttacggggta atgatcaacc ggaaaaaata caatgtgttt tatgatggct ttgcctccaa
    26581 tgtgatgccg gatatcacag tagtgcctgt tatggaatag gagggaaacg tatggagcgg
    26641 aaaacgggga aaagaggtca gctgatgggc ggccttttta ccatgctgct gtttctggta
    26701 tttattttaa gcgctctttt taccgtgctg acaggaagcc gcgtgtatga aaatatcact
    26761 gccagaagcg ataaaaattt cagcagctcc actgccttaa gctatatagc aaataaggtg
    26821 cgtcagggag accgtgccgg gatgacagac ataaaagaaa tagaaggaac gcaggtgctg
    26881 gagcttcgcc agcagattgg cgggacagat tatgtgacct ggatttactg gctggacggc
    26941 agtatcaggg agctttttac tgataccggc agcggccttg gactggagga tggtctggaa
    27001 attttggaat gtgaaggact gtctttttcc agagagggaa atgttctcca tatccgtacg
    27061 gaaggggaga acgggggaac tctgtctctt tctcttagaa gcggcagtct ggctgaaaat
    27121 cctgtttttg gtgagtcgga aagcgggggg atagaaggat gaacagaaga agcgggtccg
    27181 gttcaggacc gtttttgatg gagatgctgg cagtcgtcgg tttttttatt gtctgtgcct
    27241 ccatctgtgt ttctgtattt gcaggagcag accggttgag ccggaaggca gatgatctga
    27301 atcatgctgt gcttgcggcc cagagcctga tggaagagct gaaatccggg aaacgagagg
    27361 aagagcttca gacggtctgg gataaggact ggaaggcata tacgcaaagg gaggcggcag
    27421 agtcaggagc tgttttggcc tatgaggcag agatcacagt agaaatggat gattctatga
    27481 agcagatccg ggtggatgtg acaaaagggg ataaagaaac agacggtact gtgatctaca
    27541 gcctggaagg cagccagtat gagccttgaa aaaagaagcg ggaggaaata agccatggcg
    27601 gaacaaacat ccagaaataa agtgaatata gggctgtcat cgctgatcct gatctttatc
    27661 gtgctttgtc tgtctacctt cggccttctt tctttaagca gtgccagagg cgatctggag
    27721 ctggcagaga ggggcgctca ggctgcaaag gaattttatg aagcagattc caaaggacag
    27781 cagtggctgg ctggatcaga ccggatgctg cagcagatgg cagggcggtc tttagaggaa
    27841 aagttggagg ctctggcggc caagccggga aatggttatg atgagcagac cggccttatt
    27901 tttacagaaa ttcccatgga gcggggagag tccctgcaca ttgatctgga gttaaaagag
    27961 ggggacaaac ggtatgagat ccggtcctgg tatgtatatg acagcggtga atatgaaatt
    28021 gatgatacag tgaaggtctg ggacggcaaa gatcgttcgg gtacataata aggaggaaac
    28081 gatatgaagg taatggagct tcttacagcg gcagtggagc aggaggcagc tgatatcttt
    28141 ttgataccgg gccgtccttt ttcctataag attggcggca ggatcatctg cgagggaaca
    28201 gagaagatca tgccggatga aatggacgat atgatccgtc agatctatga gctggccggc
    28261 ggccggagcg aagaacgtgt attaaatgag ggagatgatg atttttcctt tgcagtaccg
    28321 ggggtatcgc gtttccgcgc caatattttc cgtcagagag gttctctggc cggcgttatc
    28381 cgtgttgtgc gatttgagct tccggatgcg gatgcgctgc atcttccaaa ggagattatg
    28441 gatgtttcaa ggatgacgaa gggaatggtg ctggttacag ggccggcggg aagcggcaaa
    28501 agcaccacgc tggcctgtat cattgacgcc attaacgagt caagagaagc acatgtgatt
    28561 accctggaag atcccattga gtatcttcac cgccacaaaa agagcgtagt aacgcagcgt
    28621 gagatcgcta cggatacaag cagctatgta accggcctga gagcttctct gcgtcaggct
    28681 ccggatgtga tcctgctggg agaaatgcgt gactatgaga ctatcagcat cgcaatgacg
    28741 gctgcagaga caggccatct gatcttgtct acccttcata ccatcggcgc ggccaatacc
    28801 attgaccgtg tcattgacgc ttttcctccg gctcagcagc agcagatccg cacccagctt
    28861 tccatggttt taaatgcggt catttcccag cagctagtac ctactgtaga tggaggcgta
    28921 cagcctgttt ttgagatcat gtttttaaat aatgcgatcc gcaatatgat ccgcgaatcc
    28981 aaaatccatc agattgacgg cattatcgcc acttctcagg aggagggaat gatttccatg
    29041 gacaacagcc tgatccgcct gttccgccag ggagttataa gcagggaaac ggctgtgacc
    29101 tacagcagca acggagaact aatggaacga aagctgtcca gataagcata aaatcattat
    29161 gaaaaggaga taccatttat tgaatgaggt atcttctttt ttaaatctgg aaaggagctt
    29221 tgcatgaggt cggataaaag aaaagtggca gttgtcggaa cgggaatggt gggtatgagt
    29281 tatgcttaca gtcttttaaa ccagtctgtc tgcgatgaat taacgctgat cgatatcaac
    29341 aagacaaggg cggagggaga agccatggat ttaaaccacg ggcttgcgtt tgcagcttcc
    29401 aatatgaaaa tatcagcagg aaattatgag gactgccgtg atgcagatat tgttgtcata
    29461 gcggcgggag tggcccagaa gccgggagaa agccgtctgg atcttttaag gcgaaataca
    29521 gaggtcttcc gttcgattat agaacccgtg atttcctctg gctttaacgg tatctttctg
    29581 gtggctacca atccggtgga tattatgaca aggattacct gtgtactgtc gggatttaat
    29641 cccggaaggg ttctgggaag cggaacggct cttgatacgg cacgtcttag gtatctggtg
    29701 ggaggatatt tgagggttga tcccagaaat gtccatgcgt atgtaatggg agagcatgga
    29761 gacagcgaat ttgttccctg gagtcaggcc tttcttgcaa ccaagcctat tttagagttg
    29821 ggaaaaaatg ggaaaaatga gttgagaagc tgtctggatc atattgaaga agaggtgcgg
    29881 acggcagcgt atcggattat cgaggcaaaa caggccacct attacggcat tggaatggct
    29941 cttaccagaa ttaccaaggc tattttggga gatgagcaca gtgtccttac cgtatctgct
    30001 atgcttcggg gagattacgg tcagagagac atctatacag gcgttccctg catcatcaat
    30061 caaaatggag tccagcgtgt tttggagctt tcattgtcgg aacaggagat ggaaaagttt
    30121 gggaaatcct gtaaaaccct gagggaaagt tttgagggga ttgtgtgagc aaagcaggcc
    30181 gcggcatatg cagcgtttgg gaagggcata cctgtacaat atggaatatt tatgttattg
    30241 tatagaggta tcaacatcat tgtcttccca taaagaaagg atggttttat gccattattg
    30301 aaagaacggt atacctcaaa ggattactgg gcgctgcctg aaggatgtcg gtctgaactg
    30361 attgacggac agctctatga tatggctccg ccaagcagga ttcatcagga gattgtttcc
    30421 ggtattctct atcttttaaa aaaacatatt gaatcaaaaa agggcggctg taaggtctat
    30481 cccgctccat ttgcagttaa tctggacgct gatgataaaa actgggtgga accggatatt
    30541 tccgtcatct gtgatcccga caagctgaca gaccgcggat gttctggagc tcctgacttt
    30601 atcgtagaag tagtatcgcc cggaagccgt cggatggatt acgcccataa ggtttccctg
    30661 tatctggagt ctggagtccg ggaatactgg attgttgatc cgttaaaaaa atgtacaaca
    30721 gtatatctga atgaacagga tccgtcagag tttgctccca ttatttttcc ttttgaaagt
    30781 aatatcaagg tgggaatcta cgccgatctg tctatcaaca tcagtcaatt actgtgacgg
    30841 ctgccgatgg gaattttgcc cggttttgta ccgggcattt ttgataaaag aaaaaaccct
    30901 tgcaaacaca agggtttttt acagctactg acgggaatcg gacccgtgac ctccgcacta
    30961 ccaatgcgac gctctaccga ctgagccaca gtagcaactt tctttttctg tcccgtaaga
    31021 actcttgtct cacgcgactt cgatataata tcatatgttt ttgtattttg caatactttt
    31081 tttaaaaaaa ttaaaaaatt ttttgtatca ggatcatata gcataaagtt cctgtaaaaa
    31141 tgctgattaa agtattcttt ttccaaaggt gtgcacctgc tgtcacagcg gatgcaatca
    31201 gctcaggaag cccatggggc caggttaggg gggtgacgcc ttttaagcag tagacgatca
    31261 ggaccgccat gatggccgga ggaagcacgg ttcccagata gcggatcagt tccggaagct
    31321 gccgtttccc tccaaaaaga agaaaaggaa accagcgggt tgcctgggtg cacagaacac
    31381 agattaaaag ggtgatgatt atataggaag tattttgcga agaatgtatc atgagaaatc
    31441 ctccatattt ctttgtatgg tatgcctgaa tataagaagc agggctgata cggcaataag
    31501 ggccggcagg ataaagccgg agggaccgaa aagagccaga cagagcatac cgcagaaaaa
    31561 acctgtttta gccggcagat gacagcttgt ttcccgccac tggttgacac agattacaat
    31621 aaagagagca gtcatggcaa aatcaatgcc tgtggtgtca aatggaagaa attgtccgat
    31681 gagggctcct gccaccgaac cggccaccca gtaacagtga ttaaaaaggg agataaaaag
    31741 ggaagaaagc ttccattcac ggtctgtaaa atcatccggc tttttggcgg agcacaggat
    31801 ggaatacgtt tcgtctgtaa gggaaaagat catatagggg ccggctgctt tcattttacg
    31861 aaaacgttca ataaaggtca ggccataaaa tgcgtgtcgg ctgttgatca aaagcgtcat
    31921 gatggctgtt gcggcaaaac tcagcccttc tgtgagaatc cctataaggg caaactgcat
    31981 ggaaccggca tagacaaaga gactgatgag aaagacccag ccggctccaa gaccggcctt
    32041 ttggagaagg aggccaaagg caattccaag aaaaatatat cccagaaaaa cagggattgt
    32101 ttttatcagg gcaaatccag ctgctttttt catagatgct cctttccgga ttgaaatagt
    32161 tcaatcactt ttgcacgttg atttagtata gcagacgaaa gaaaaaattt ccagtcttaa
    32221 gtgtcaggga ttcttatttc ttaacgtttc cctcttttct ccctgaaaaa aatgtgatat
    32281 gattattcaa tacacgggaa tcatttgggt tccatgatgg aagagaaatg tacggaggag
    32341 caatcatggc agaaaaagac atggtattgg agacagggga gaaagaaact gtttccaaga
    32401 attttataga acaggagatt gacaaagatc tggcagaggg cgtttatgat aatgtccata
    32461 cccgttttcc gccggagcca aacggctatc ttcatatcgg acacgctaag tccattcttt
    32521 taaattacgg actggctcag aagtacgacg gcacctttaa tctccgtttt gacgatacca
    32581 atcctacaaa ggaaaaggta gagtttgtag aatccatcaa ggcggatgtg aagtggctgg
    32641 gtgctgattt tgagaaccgt ctgttttttg catccgatta ttttgaaaag atgtatgaat
    32701 gcgctgtctt cttgattaag aagggaaagg cctttgtatg cgatctttcc gcagaggaga
    32761 tcagagagta ccgcggcgac tttaacaatc ccggtaagga aagcccctac agaaaccgtt
    32821 ccgttgaaga aaacctgcgc ctttttgagg agatgaagga aggaaagtac aaggacggtg
    32881 aaaaggtgct ccgtgccaag atcgacatgg cttcagccaa tatcaacatg agagacccgg
    32941 ttatttaccg tgtggctcat atggagcatc acaatacggg aaataagtgg tgcatttatc
    33001 ccatgtatga ctttgcccat cccattgagg acgccattga gcatattacc cattccatct
    33061 gtacactgga atttgaggat cacagacctc tttacgactg ggtcgtaaag gagtgcgagt
    33121 ttgagaatcc gccccgccag atcgagtttg ccaagctgta ccttacaaat gtagttacag
    33181 gtaagcgtta tatcaagaag ctggttgagg atcatattgt ggacggctgg gatgatcccc
    33241 gtctggtatc cattgcagcg ttaagaagaa gaggatacac accggagtct atccgccgtt
    33301 ttgtggagct ggtaggcgtt tccaaggcaa acagctcggt agattatgcg atgctggaat
    33361 actgcatccg tgaggactta aagcttaaaa aagcccgtat gatggctgtg cttgaccctg
    33421 taaagctggt gatcgacaat tatcctgagg gtcagatgga agagctggat gtgccgaaca
    33481 atctggaaaa tcctgagctt ggcagccgta aggttccctt tggccgtgag ctttatattg
    33541 agagagagga ctttatggaa gagcctccga agaaatattt ccgcatgttc cccggaaatg
    33601 aagtccgcct gatgggcgct tattttgtaa agtgcaccgg ctgtgagaag gatgcagatg
    33661 gaaatgtgac agtggtacat ggaacctatg atcctgagac aaagagcggt tccggttttg
    33721 agggaagaaa ggtaaaggga accatccact gggtagctgt tcctactgca aagaaggtag
    33781 agtgccgtct gtatgaaaat attgtggatg aggaaaaggg caagttaaat gaagacggaa
    33841 gcctgaactt aaatcccaat tcactgaccg tactgccgga ctgctatgta gagcctgcac
    33901 tgggagaggc agaaagctat gacagcttcc agttcgtgcg caatggttac ttctgcgtgg
    33961 actgcaagga cagcacaaag gaaaagcctg tatttaacag gattgtttca ttaaagagct
    34021 cctttaagct tcccaaataa tgcagcagct gtgtgagaaa cgataagatt ttataaaaag
    34081 aataaaatcg gctttgccgg aaaaagcagg aggaggctct ctggaaacag ggggcctttt
    34141 tctgtacgtc cctgatgaaa ggagaaaatg atggaactga tggtgcctat tgataatcag
    34201 agcagacttc ctctgtatga acagatttac caatatatga aggaagagat acagagcggg
    34261 cggattcctg caggaagcag gcttccttct acacggattc tggcagaaaa tctgaaggtc
    34321 agccgcagta ccactcaaat ggcttatgaa cagcttcttt ctgaggggta tatcgaagcg
    34381 ctgccctgta aaggatactt tgtatgcagg atcgaagaac tggttcgggt ggaaaaaaat
    34441 gcagatgagg aatttgcgga aaacagatct gaaaaagaaa tgccgtatgc tgtggatttt
    34501 tccaccagag gtattgatct ggacagcttt ccctttaata cctggcgcag gttaagccgc
    34561 aatacgctgg tggacgataa taaggaaatg tttgccgtgg gggatccgca gggagaatat
    34621 gagctcagag cagcgatccg cgattatctt cactcggcca gaggggtaag ctgccgtccg
    34681 gagcagatcc tggttggagc aggaagcgag tatcttctca tgctgctgtc gcagcttttg
    34741 gggagagaga cgggaattgc cctggaaaat ccaacctatc gtcaggctta ccgggtacta
    34801 aagagcctgg gacatctagt tgttcccgta aaaatggacc ggtatggcat ggatgttttg
    34861 gatctggaac agagcaaaac tcaggctgct tatgtaatgc cgtcccatca gtatcccaca
    34921 gggatcgtca tgcctgtaaa gcgcagacag gagcttttaa gctgggctta cagaggagaa
    34981 aacaggtatg tgatcgaaga tgattatgac agtgaattcc gctataaagg acgtccgatc
    35041 cccgcacttc agggcatgga ccaggggggg agggtcattt atatggggac attttcacgt
    35101 tccattgccc cggcgatccg cgtgggattt atggtgcttc ctggagagct cctttcaagg
    35161 tatcggagcc aggccggatt ttatgcctgt acggtttcac ggatcgatca gaaggtgctg
    35221 tatcatttta ttacaggcgg gtattatgag cgccatttaa accggatgcg ggccgtgtat
    35281 aaggggaagc atgacgctct gttaagcggt cttaaagaac tggaagatat gttttggatc
    35341 aggggagaat atgcggggat ccatgtgctt ctcacacata aagggcagat gccggaaaaa
    35401 gagctgatcg aacgggctgc cggtgagggc gtacgggttt atggcatgtc ggaagccgtg
    35461 ataggggaaa ataaggatct ggtgccgtct acagtgattt tgggatatgc caatttaaag
    35521 gaaaaggaaa tacaggaggg ctgtatgcgt ctggcaagag cctggaaatg agtacatgga
    35581 cagagagcct gaggaaagca gagaaaggag cgcaagatga agagaaagag aaaggaacga
    35641 tacaggttat gggctgctgt tgcagtgctg tatgcagtgg tgctgccggt aagtggactg
    35701 atgggaatga cagagaatcg ccaaaatgcc cggatgcaaa gcgaatcggt cagagaggtg
    35761 gagccggtaa ataagcaggg cgcagtgaaa gagaatgaag aaaatccgac cggttcggag
    35821 actgtttttg gagaactaag ccttacggca gagcagcttg acgcggcaga ggcggttttt
    35881 tcttcccttg aaagcctgga tctggaacag gcggcccggc tcatggacag agagcaggac
    35941 gttctggcag acctgtttta tgagacaatg ggaggaacac ggtatctgta cacaccatcc
    36001 ggctttactg ctcagatcga gggagaagga atggtattta ccatgccggg aacggtattt
    36061 tatggttatt ttaaagatgg aaagcccagg ggggaatgtc tggctcttca gtttatcaat
    36121 gtagacagcc cccgttacaa ttattcctat ggaatctgga gaaacggcag gatgacgggc
    36181 tatggaagca caggatactg ttattatgaa aatgtgcctc agggagagat ggctaaaaca
    36241 gtaaagcagg ggaattttaa aaatgattta ttagaagggg aaatttccta tgaaactact
    36301 ggtgcagacg gaacggtatc ccactggtat atggcggcat cagaaggggc gacagtgatc
    36361 gatacccgct ggacctggat ggaggaggag aaagagtatc agcttatgtc tgccgatgcc
    36421 cagagccatg gctacttcat tagcgaggaa caggctggtc aagtgatgtg gatgaacctg
    36481 ctggggtggg aagtataagg ggaaaataaa gggacaaagc ctgaaaatag ctttgtcccg
    36541 tatataaacc taaaccagcg gctcatcatc ctcagtgaga atggtcccct cttcatcttc
    36601 tgcaagttcc actgaaacta catcctctgt ttcatcatca tcttcttccg aagaaccccc
    36661 atcttcggtc tcagaaggct gtttttccgc atagactggt tcggcatcca gacgttcata
    36721 gtcatagaga tcatcatcgt ctacataatc gtcatccagg aagccgtcct cctccatgtg
    36781 tacctcttcg ggctcatcct ccttttcatg ctcctccttc cattcctctt tttttgactt
    36841 catggaattc agtgctatat gtgcggtgtc cacagcggca gatacagcct catgggccgt
    36901 atcagagaac aggcttcctg cgtcccggag cacatttttt gtagcctgag ccatatcctt
    36961 ggcagctact ttaaattcgt ccttgctgga ggtaagagaa atataattgc ggtttacttt
    37021 cgctgattca aaggtactgt cttcagatcc atcttttttt aattcactat cctcatcttc
    37081 ttcaaattcc ctgaaatcct tttccagctc tttgtgatag gtcttatact ggagtacata
    37141 ggaaacaccg gctgccacag ctccggatac ggcggctaaa gcgaccagtt tcccccaacc
    37201 tttttttgcc atataaattc ccctttcttt gataagttgt ctgattatga ctgtttgcta
    37261 ttattgtaat acgaatgggg aaaaaaataa acatgaaatc ataaaaaaaa tattaaatta
    37321 caggaaaatc atccggagaa agaaaatttt tatcagaata aaacggttct gaattttaaa
    37381 ggcggaatgc cctcctgttt tttaaaaaag ctgataaaac tggattctcc tccaaagccg
    37441 cattggcagg cgatttcgtg aacgggaagt tcggtggttt ttaaaagctc ttttgctttg
    37501 gaaagacgaa gaaacagcag gtattcatag ggcggaacac cggtctggcg tttaaactgt
    37561 ctggaaaatt gggaaggact caggctgact gcctttgcca tatgttcaac cgttattttt
    37621 tcggaataat gtgccttcat atagcggacc gctgcaaagg acaaactgtt ttctccctca
    37681 gaatccatgg tttcttcaaa caggcctgtg atcagtgtat atatcctgga ggataagcgg
    37741 ctgatgctta tggcttctct ggcttttata agctcaaaaa gctcacagat atgggcagga
    37801 attacagaag ctggggaaat aatgcagcct cttttgatca tgagctcctc aaaaagctct
    37861 tttgtattgc tgccggagat atggagccag tatgtttcca gatgattgtg ggtgcagtac
    37921 atgtgaggtc caaagcagtc taccagagcg atttctccgg cttttgctgt gagctcacgt
    37981 ccctgatata aaaaagaaaa ggtaccgcgg acgatgtaag cgattaaaat actgtcaaag
    38041 gagctgcggt ctacccggta tccactggta cagtaaaaat ggcctgcgct taaagggtaa
    38101 aaaaagagcc tgggattaaa agcgccggaa tagaaaaaag ctcggatgac tgtaagatac
    38161 ctttttgcat attcctccta ctctgccata cagcaggatt ttgaaattat tcggcggtat
    38221 ttcaaaagaa atttccgctg cttcattgta tactaaaaca gaacaggggt caatcaatca
    38281 gcctgtgaat ctttaagtct atataacaga ggtggcagag atgaagtgta ttacagaatt
    38341 aaaggattgg gattttaaaa tcccggagct tggatttgaa aaaagagtga cgcttcctta
    38401 tacatggaat gtggaaaatg agcggccggt acagctttac agaggaccgg cagaatatat
    38461 aacagaaatt gaactggatg aaatgccatg ggacagggca gtgctgtatt ttggttgtgt
    38521 atttcatacg gccagggtat ttgtaaacgg aataccggca ggaggccata caggctctgg
    38581 atatacgccc tttgagttgg atataactga tttggcagta cgggggaaaa accagatctg
    38641 tgtagtcgca gacaacagta aaaaagagga tatgctccct catggactgg attatgactg
    38701 ggcggatgac ggagggctgc tccgggatgt atgtctgatc cggatggaaa aggacgatat
    38761 tatggaaatg aaaacagggt atgccattga atccataagc ggacagacct gcagcggtta
    38821 tctggagttt tatataaaag gaacagcgaa agaggccctg gttcaggtgt cggagtacaa
    38881 aaccggacac atagtgctgt ctgaaacgat ccatacagac acgctggtcc gtatcccctt
    38941 tgagggcctt aagctatggg cgccggagca tccccggctt tatgtggtgc aggtgcgcac
    39001 aaaacaggat atggcttctg aacggatcgg cctgcgcacc atagaagtaa agggagcggg
    39061 cgtatatctg aacggaaagc gaatttttat gaagggctgt gagtggatgc cgggttctca
    39121 tccggattat ggcatggcag agctctttga gcacagcaga aaatgcctgt ctcagttaaa
    39181 acaggcgggc tgcgtattta cccgtttcca ctggcagcag gacagaaaga tcctggactg
    39241 gtgcgatgaa aacggactta tggttcagga ggagattcct ttctggggac agccaaagaa
    39301 agccggatca gagcagcttg cgctggcaaa gcagcaggcc ggggaaatgg tgcattatca
    39361 cggcagccat ccgtccatta tctgctgggg cgttggaaat gagcttgggg gagaacttcc
    39421 ggaaaccatt gcgtatgtaa aggagatgta ccggtatttt aaagaactgg atggcagcag
    39481 gctggtgaat tatgtttcca atacggtttc cagagatgca aatgttgatt tggatgatgc
    39541 ggccatgtat ggcgatattg ccatgtggaa tgagtatctg ggtctctggc agccctgtga
    39601 ggatgtagaa ggggtgctcc gaaggacgta tgagaagttt ggcggtatgc cgtccatggt
    39661 tacggaattt ggattgtgtg agccagcttt tgaagggggc gatgagagaa gggcgcagat
    39721 tttaagagag cggatcccgg tatataaaag cctcgaaaaa atggcaggct atgtatggtt
    39781 ttccttaaat gactatcgaa ctcactatgg ggaatatggg gaggggaaaa tgaagcagcg
    39841 gatccatggg tcgacggatc tgtatggaaa tgaaaagcct tcctatgagg tgttctgtga
    39901 aattgagtaa aatggcgttg ttttagtcat atttgagtaa gtggcgctgt atgatctaat
    39961 gatacttcac aaatatttta gaaaatatta gcactcaact cttgacagtg ctaataagca
    40021 gtggtataac ttacattgtg aacaaaagaa gcaggaaaca gcaaaagctg agagaaaata
    40081 atgaatgatc acaaggagga atccagatat gaagttagta ccattatttg acaaggtcgt
    40141 attaaagcag ttagttgcag aggagactac caagtccggc atcgttcttc cgggagcagc
    40201 aaaggaaaag ccacagcagg cagaggtaat cgcagttgga ccaggcggcg tggttgacgg
    40261 taaggaagtg gttatgcagg taaaggccgg cgacactgta atctattcca agtattcagg
    40321 aacagaggta gagctggatg acgagaagta tgtgatcgtt aagcagagcg acattctggc
    40381 agtagtagag taatcattaa aaatacggac ttatttataa atttggaggt tgattgacat
    40441 ggcaaagcag attaaatatg gcgtagatgc ccgcaaggca ctggaagcag gcgtaaatca
    40501 gttagcagat accgttcgcg taaccttagg ccctaaggga cgcaacgtag ttcttgacaa
    40561 gtcctttggc gcaccgctga tcacaaatga cggtgttact attgcaaagg aaatcgagct
    40621 tgcagatcca tttgagaaca tgggcgcaca gctgatcaag gaagttgctt ccaagacaaa
    40681 tgacgtagca ggcgacggta ctaccacagc aaccgttctg gcacaggcta tggtaaatga
    40741 aggcatgaag aacctggcag caggcgctaa cccgatcatc ttaagaaagg gtatgaagaa
    40801 ggccacagac gcagctgttg aggcgatcaa ggctatgagc aagcctgtag gcggcaaggc
    40861 tcagatcgca agagtagcag ctatctctgc atcggatgat gaggtaggta caatggtagc
    40921 tgacgccatg gagaaggttt ccaaggacgg tgttatcacc atcgaggaat ccaagaccat
    40981 gaagacagag ctggatctgg tagaaggtat gcagtttgac agaggctacc tgtccgcata
    41041 tatgtgcacc gatatggata agatggaagc aaatctggat gatccatacg ttctgatcac
    41101 tgataagaag atttccaata tccaggatct gctgcctctg ttagagcaga tcgtaaagat
    41161 gggcgcaaga ctgctgatca tcgctgagga tgttgaggga gaggcattaa caactctgat
    41221 cgtaaataag ttaagaggta ccttcaatgt agttgctgtc aaggctccgg gctacggcga
    41281 cagaagaaaa gagatgcttc aggatatcgc aatcctgacc ggcggtaccg ttatttccga
    41341 agaggttggt ctggatctga aggatgctac cttagatcag ttaggccgtg caaaatctgt
    41401 taaggtacag aaggaaaaca ccatcatcgt tgatggtgag ggcgataagg aagcgattgc
    41461 agcaagagtg gctcagatcc gcaagcagat cggtgagact acctctgagt tcgataagga
    41521 gaagctgcag gagcgtctgg ctaagctggc aggcggcgta gctgttatcc gtgtaggcgc
    41581 tgctaccgaa acagagatga aggaagccaa gctgcgtatg gaggatgcat taaatgcaac
    41641 ccgtgcggca gttgaggaag gcatcatcgc aggcggcggt tctgcatacg cacatgctac
    41701 cgtagatgtt cagaaggtag tagacggtct ggaaggtgat gagaagacag gcgctaagat
    41761 catcttaaag gctctggagg ctcctctgtt ccacatcgtt tccaacgcag gtctggaagg
    41821 cgctgttgtt gtaaataagg taaaagagtc tgaggttgga tgcggttttg acgcatacaa
    41881 tgagaaattt gtagatatga tcgaggctgg tatcctggat cctgtaaagg ttacaagaag
    41941 cgctctgcag aacgctacca gcgttgcatc tactctgctt acaacagagt ctgttgtagc
    42001 agcgatcaag gagccggctc ccgcagctcc tgcagcacct gacatgggcg gaatgtacta
    42061 atttccctgt caaaactggt aaaatataaa actacggatt ccctctggat ctgggattta
    42121 ttctcagaac caggggggat ttttgcgtaa atgagggttt ttgagaaaag aaacgggtac
    42181 agccttaatg aaaatgtaat acttctgtgc ttttttgtaa aagaaaactg tgatatactg
    42241 gaaaaataca ttggatagaa aacgaggtac ttgatatatg aaaccataca gatggaagcg
    42301 ggttcttggt atatgtacag ctgcggtctt ggggctgtct tttccggctt atgcggtaac
    42361 gattacgcct ttaaatccgg gaacaggaca aagctcttcc tcagagcctt ctggaacggg
    42421 agcacctgca tcacaggaaa atacagaacg tttaaatatt gcccagccgg ttgtgtcctc
    42481 tgagggggcg gtgctcatgg acggggcttc gggaaaggtg ctgttttcca aaaatggaga
    42541 cacccgctat tatccggcca gtattacaaa gctgatgacg gctctcttag tggcagaaaa
    42601 ctgtagcctg gatgatacgg tgacattttc agcatcagct actacaaatc tggaatcagg
    42661 agctgtttcc atcggtatgg tggagggcga taccatgacg gttcgccagt gtctgtatgc
    42721 cctgctttta aagtctgcta acgaagtagg aaatgcgctg gcagaacacg tttcaggtag
    42781 tgtccaggca tttgcagaca ggatgaacga gaaggcggtt aaacttggct gtaccaacac
    42841 ccattttaca aatccccatg gattaaatga tccggagcat tataccacgc caaaggacat
    42901 ggcgctgatc gccagagctg cttttcagaa tgaggtggta aggacagtag cgtccaccag
    42961 gacatacagt cttccggcta cgaaaaagaa tccttccggg cttacggtca caatgggaca
    43021 taagatgtta aatcccaatg attcccgtta ttatgagggg ataatcgggg ggaagacggg
    43081 ttatacctcc aaggcaggaa atacactggt aacggcggca gaacggaatg gagtccgcat
    43141 gatcgcggta gtgatgaaga gccgttccac ccactatgtg gatacaaagg ccatgctgga
    43201 ctatggattt gagctggcaa aggcaggagc gcttgaaaca aaccagactg ggggacagaa
    43261 tgcaggttct tcccagacag gagcgggaag cggcggtccc ggagctggtg ccggcccatc
    43321 gggagcagcc ggtgctcccg aaagtacagg agcatcagga gtaacggttg taccgggaaa
    43381 tacgggtgct ccccagaacc caggaagcac gggtgttccg gtcacaggta cagagggcgg
    43441 acctggtgtt tcttctcagc acggctgggc aaagacggaa aaaggatgga attttgtcaa
    43501 ggataacgga gcaaaagcca gtggggaatg ggtttctgat gcagaaggaa cctattggat
    43561 cgatgaagat acatttatgg caaaaggctg gcgccagatt gatggaaagt ggtactatct
    43621 gcgttccaac ggtgctatgg caaaaaacta ctgggaagaa agccaggatg gagtttggta
    43681 ctatctggga gcagacggtg tgatgatgac aaatgccaca accccggacg gataccgggt
    43741 aggagcggac ggagcctgga tccggtaggg gatatttccg tatacaggat gatcaactgg
    43801 caatcgatta taaaaatgcg gcagactgtt tgtgtgggtc tgccgcattc ttcatgcagt
    43861 gtgcctgatg gcatgctgtt ttattatttt acgataactg cctgatcagt tacttctacg
    43921 gtctttccat ccgccagtcc aacggtcttt acagaagcaa agctgccatt gatctgatcg
    43981 gcagtcagaa cggtcatgcc ggaagctggc tgggaataat tggtgaaatc aaggatcagg
    44041 tcgccgcctg catttacaat tccgcctact gcgagcatat cttcaggacc gctgatcttt
    44101 aaggttaaat caccctcagc gccctgtgta aagtcgccgg agatattaag cgcgccgttt
    44161 acattttctg taatggctgc atggttgtct acagagctgt atccgaaggc gtaaggagaa
    44221 gctgcggtga gcgttcctcc gtttacatgg acgccgccgg tatagctgtt atttccccac
    44281 agttccagag tgccggagcc gtttttcgta aggcttcctt ctccggcgat gtcatttttc
    44341 cactggtcaa aggcatcaaa gccgcccttg gaaccgtcca tggttacggt tacgtctgta
    44401 tcaaaggagc cgtagccgtc gcctgcttca tagaggttta agcgtcccca gccctcggcg
    44461 tcatccagaa atacatagcc ggatgggaga cctgtggaat acagaatcca tctgcgctgg
    44521 tctgcattca gataagggaa tcttgtttcc agaagaactt ctgctccctt tggaacacgc
    44581 ataggctgtg tgggatcacc ggtctgaggc atattgtagg tcatgcgttc attgtatcgg
    44641 atggcgttta attcatcatc atcatagtag gttacttcct tggcagaagg attcttaaga
    44701 aggatttctt ctgcttcttt gcgggcgtct gcctttaact gggcattgtc cggatcattt
    44761 aaggctgcgg cagccagggc cattgccatg gtgcgtccgc ccattacgtc cataggagag
    44821 tgcatacctg cgatgatgcg gtaatttcca atctcagatg cattggtaag acattcttta
    44881 aactgttccg gaactgcata ggccagagaa agggatgcca gatagcctgc gtttgtgtgg
    44941 ccgcttggga agccgccgtc ctccatgggc tttgatgata cgcagggctt taaggccggt
    45001 acgatggata cgtcgctgct ccagcggaag gggcgcatat actggaaata aaccttggca
    45061 ggattgccgc tggcatgagg gccgcggact gtatttacaa gctctaccat gctgccgaga
    45121 gaggaggttt catcggccca ggatccattt tcattgccct tatcactgta ttttttcgtt
    45181 tctgcatcag cagggatggc atccggcaga gaggttttcg catttgccag gtttttaaac
    45241 gcggtttcat aggaacccag tccgtccagt gcggaccagt tctggttctg gcggtcggtg
    45301 agataagcct gaacttcctg ctccagcgtg cggttctggg tcatggtaat gcaggtaaag
    45361 atattctggt ccagaagacg ctttccgtcc tcgccaagct tggttccgtt atcccagctg
    45421 gatcctggct cccagatgtt taagaagggg gagagaaccc ctacgactgc attagactca
    45481 ggggactggt tttccatggc atttgtcttg taggtatcta caaagtagcc atggggaccc
    45541 ggaactggtt cgatggcagc tggatcagtg gcagccataa ctggtgtggc agtcattgcc
    45601 agcgctgctc caagagcacc tgcctgcttg atgaattttg aacgtttcat agaaaaaaaa
    45661 tcctcctcgc gaatcaattt taaattcatc ataacaaaga aaaacagaca aagactactc
    45721 atattttgcg tgttttgaga aggggaatat gcattttatg cgttttggta atgccggggt
    45781 aaaattactg ttgttcatct ggggccgtag gacggtagga ggagtggtgg gaagtcagct
    45841 tgtagtccga gggagacata ccgcagaact ttcggaatac ccggttgaac tgtgaaaaac
    45901 tggaaaaacc agtggattct gctacctgcg taatacgcat aggtgaattg agcaggagat
    45961 actgggcatt tttaatccgt gtcagctgga tgtactgtac caccgtgtag ccagtgacat
    46021 ttttaaactg gtgggacaga tagtagggac tgatgtaaaa tgtctctgcc agagcggcca
    46081 gagaaatatc ttccgtgtag tgggtatgga tatagttggt aatagtataa atcttttctt
    46141 tcacaccgtt ttccacgtta ggcacgtaat ggtttagccc ctgcatttca tacaggagaa
    46201 agagaaattc cgtaaatttg gtgtgtatca gaagattgcg gatattgaca ttgggcgtat
    46261 ctttggcaat cattacgatt tcattgagct tctggtataa acggttctgt tcctctttgt
    46321 gaaaacgaaa aatgggtaca gagctgttaa aaacggaaag gatttcctta tagccggaag
    46381 caccgtaatt catctgtttt ggcagcataa atccaatgat gatacgatcg ctgggcgggc
    46441 cgggcagata ttcggactga tggagcaccg atggagggag cagcaccaga tcattggcaa
    46501 tgatatcata gcgttttccc tctacgaaat ggtatgcctt tgggcttaag aggatcatca
    46561 gttcataaaa gctgtgaaaa tgtggaaaat ccatgttgat agcgtgactt cgtttatcgt
    46621 agtcaaagta ataaaaggta gagttttcaa tgggtgtggc tacctcattg ataataatgg
    46681 aaaagccttc ttcgtagaga agcggagagt tgtggtaggt tttcataagt gtgttccccc
    46741 tgaacatagt aaacagttat atttcattca gtaaaacaca gaaaaacaaa aaatgcaata
    46801 cccaaatatc ataaagacaa aattgaaata gttattgctt taaaaagcag gtatactgta
    46861 gtataatgtt aaaaaataat aatcatagga gacaccataa tgcagatacc aggctatttt
    46921 gaggatatga attatttaca tgtaaataca gagccggatc gggcgtatta tattccagct
    46981 tctggcaaag gctgttactt tctggatcgg gagaaatcag acaggtttct ccttttaagc
    47041 ggttcctggc agtttcagta ttttgaaagc gtatacgatc tgccgcagga gttctggaag
    47101 gagggagcag gaaagggaaa agaggtgcag gttcctgccg tatggcagag ttacggcgtg
    47161 gatggaaacc agtatatcaa cacccggtat ccatttccct ttgacccgcc ctatgtgccg
    47221 agggaaaatc cctgcgggct gtatgaacga agttttgagt acaaatgctg tctcgatgct
    47281 ccggaggcat ttttgaactt tgagggtgtg gattcctgct tttatgtgtg gatcaacgga
    47341 atctttgtgg gatattccca ggtatcccat tccaccagtg aatttcacat tacccggtat
    47401 ctgaaagagg gtgaaaaccg cctgaccgta ctggtgttaa aatggtgtga cggaagttat
    47461 ctggaggatc aggataaatt acgtatgagc ggcatttttc gtgatgtgta tgtgctgaga
    47521 cgtcctaagg aggcagtccg cgattacttt atccgtcctt atatccggga aaatggggca
    47581 gaggtttgta tggagctgtc cttttatggc aacagccgtc ctgatggccg gatccgtgtg
    47641 cttgacccgg agggaaaggc agtctgggaa cagagctgga acgaaacagc agatagggat
    47701 caaatacggt tttatctgga aaactgccgt ctgtggaccg cagaaacgcc ctggctttat
    47761 accgttgaga tccagaccga aaaggaatgg atcaccgacc gatttggcat ccgtgagatt
    47821 tctgtggaaa acggtgttct gtttatcaac aaaacaccgg taaagttcca cggagtgaac
    47881 cgccatgaca gtgaccctgt tactggctat gccataagca gacagcagat ggaacgggat
    47941 cttaagctga tgaaggagca caatgtaaat gccgtccgta ccagccatta tccaaacagt
    48001 ccccagtatt accacctgtt tgacgagatg ggtttttatg taatggatga ggcggacaac
    48061 gagagtcacg gtacggataa ggtatttaaa aaggtggatg actgggatac ccatgtaaag
    48121 aagtggaacc gtccgattgc agataatcct gcatatacgg aggcggttct ggaccgtaca
    48181 agacgctgtg tggaacggga taaaaaccat gccagcgtac tgatgtggtc catgggaaat
    48241 gaaagcgcct acggctgtgc ctttgaagca gccatggcct ggacgaagga gagagacgat
    48301 accaggctgt gccactatga gggcgccaga tatgtgccgg atgtaaagaa atatgatttt
    48361 tccaatatgg atctgtacag ccggatgtac ccggatcagg aggagatcat acggtatttt
    48421 gaggagcagg aaagccgtac gttttctgaa aatcttccct ttatgatgtg cgaatatgcg
    48481 cacgctatgg gaaacggacc gggagacctg gaggattatt ttgaattgat ccaccgatat
    48541 gatggaatgt gtggcggctt tatctgggaa tggtgcgatc atgcagtaaa aaccgaatca
    48601 ggctttcttt atggcggaga ttttggggaa gagcttcatg acggaaattt ctgtgtggac
    48661 ggactggtgt ctccgaaccg gattccacat ccgggcttaa gggaatttaa aaatgtatac
    48721 cgcccggcca ggattcagag cgcagattgg gaaacgggag cgattgtctt taaaaactat
    48781 atggatttta cagaccttaa ggactatgtg tatctgaaat gggaagcggt tcaggatggg
    48841 atcatcatag aggaaagtat acagaaggag ctgccgtccg tactgcctca tacgtgcggt
    48901 acggtgtatt atacagggaa gctccctgaa aatggaagct gctttttgag agtgagatat
    48961 ctgttaaaat ctccggttcc aggacttaag gaaggctggg agcttggctt tgatgagatc
    49021 cggatgccgg agactgcggc tggaaggcag gagaaagctt tgggatgtga aagccggcct
    49081 gcagcctgct ccggcgcact cacactggag cagacagagc gtcggattac agtaaccggg
    49141 gcggatttta cctatgtgta tgataccttc tcaggagctt tctccgggat cagaaaggga
    49201 gaaaaggaat acctcatccg tcccatggag tacaatatct ggcgtgcccc aacggataat
    49261 gaccgaaaaa tccggctgga gtgggagaga gctggctatg accgcaccaa ggtgcgtacc
    49321 tataccacag aggctcgttt gagagaagac ggctctgcag agcttgtgac agagctgtct
    49381 ctgtcggccg ttcatatcca gcgtatttta aatataaaag ccagctggct gatccatagg
    49441 gatggctgta tccaggtgaa gctggatgta aaaaaagata tggaattccc atttctgcca
    49501 agattcggcc tgcgcctgtt cctggatcag agtcttgacc gggtacgata ctgcggaatg
    49561 gggccggatg aaagctatcc ggataagcat caggcgtcct ggcacggtat tttcgaggga
    49621 gatgtgcgca ctatgcacag gccttatctg aagcctcagg agtatggttc ccactgggac
    49681 tgcagctggt gtgagatttc aggagaagag atgagctttc aggcttcatc gaaagaaggc
    49741 tttatgttcc aggcctgcga gtatactcag gaggaactgt ccgaaaaagc acatgacttt
    49801 gaacttaagg aggtaggatg cactgttctc tgcttggatc acaatcagag cggcatcgga
    49861 tccggaagct gcgggcctca gctgctggaa aaatatcagt tcaatgagcc tgaattttcc
    49921 agaacctggg ttataactcc gggaaagcgc aaagaataaa caggagagaa aacagaaaat
    49981 gaaacgaaca aagtggattg catttttatg ctttgctttt ttaatggcag gtcttggagc
    50041 aaatgacgcc cttcgaggga tctttgcccc tgtgttccag gaacggtacg atttaaacgg
    50101 gagccagctt tcgttgattg ttactatcag ttatgtggga aatctgctgt ttttaagcat
    50161 cggagggaag cttctggatc tgtttcccag aaagaatgtg gctgtggcgg taacggctgt
    50221 ctgggcggga gcagttgccc taaatctggt gtccgacagc tatatatgga ttctggtatc
    50281 catgtttttt gctctgggag catccaccct tttaaatact acggtcaata tattgactcc
    50341 ggcggtgttc gcaggatatg cgggccttat ggtaaatgtg ctgtttttta tccaggggat
    50401 tggcaccagc ggaagccagc ttctgctggg aagatacgcc ttccactatg caggatggaa
    50461 atggattaac gctgcccttt tgactgtggc ctttctggta attgtcctgt ttttcttttt
    50521 aaagatccca aaacagggag aggagaagga gcgggagaag gaaattctta aaaaagagcc
    50581 ggattcaaag atattctgga tttttattgc catgatgggc tgttatttta ttggggaaca
    50641 cggcatcatg aactggctga tgtcctactg tatctctgca ttttccatgg aagcctccag
    50701 cgcttctgca agcctgtccc tgttctgggg cggcatgacg gcaggacgtc tgatgtttgc
    50761 tcccgtggtt cagaaaatgg gcgcggtaaa gagcatgaag ctctttggcg gtatcggaac
    50821 ggtgatgttc tgcgccggct gtatcctggg agagagcggc gtatggattc tgggagcatc
    50881 aggctttgcg ctggcgattt tgtatcccac catggtgctt ttgatccagc agatttatcc
    50941 ggttcagacc gcagccaccc gtacaggcgt tatcatcagt gcggctacgg tagcagatat
    51001 tgtgtttaat ctgctctttg gcgcagtttc ctccaagtgg gggtatggca tcagctttat
    51061 gatccttcct gtggctatgg ctgtttttta tggtttgtat ctgaggctcg caggaagttt
    51121 gaaaagaaat tgaggaaaaa tgaaaataga aaagggagaa cgggcagatg gatgtaatga
    51181 gaaacagaat acggccgatt ttagacagtt tgagacagtg gcgctgccgt ttcagtgaag
    51241 aactgacaga ggtagaaatc cgtccgggcg attatcattt ttcagagaag ctggatgcag
    51301 atcagctgaa agacggatgg atgccttttg gagcttgtga aacatgggga ggaagggacc
    51361 gacatttctg gttcaggaca aaggttgtga tacgaaagga atgggcggga aaagaagtac
    51421 gggtgaccct gaacacgggt gcagatgata tatggaatac agataatcct cagattatgg
    51481 catataaaaa cgggatttta tcgggaacca tggatatgaa ccatcaggat ttgatcttgg
    51541 cagaagaggc ggaggaggca gaggtttttg aactggtatt ttatgcctat tccaacagtg
    51601 aaaatccgac aaactttttc catctgaaag ccgctgttta tgacagggaa atcgcagatc
    51661 tgtactatga tttaaaggtt ccttttgagg cggcagagct ttgttccgag gaggatctgg
    51721 atcgcattga ggccatgaaa tttttaaata ccgccatttc cggtctggac ttgagaaagc
    51781 ccggttcaga ggaattttat cagtctgtaa gggaagtgcg ggaatatgtg aaaaaaaatt
    51841 tgccagatat atgccgcagc agccgggtaa ccgttcactc cattggacat acccacattg
    51901 atgtggcgtg gaaatggcct ttgagacaga ccaggcagaa ggcagtccgc agctttcaaa
    51961 cggtactgaa tctgatggaa cagtacccgg aatataaatt tatgtccagc cagcctcagc
    52021 tttatgagtt tgtaaaggag gaagctccgg agctgtttga gaagatcaaa gaaaagattc
    52081 acgagggacg ctgggaggca gaaggaggaa tgtggctgga accggactgt aatctggctt
    52141 ccggtgagtc attgatccgc catattctct acggaaaacg tttttttgaa aaggaactgg
    52201 gagctcaaaa acaggaagtg ctctggctgc ctgatgtgtt cggctacagt gcagccatgc
    52261 cgcagatcat gaaaaaatca ggcctttcct attttatgac aacaaagctt ggctggaatg
    52321 agtacaatgt atttccctat gataccttta tgtggaaggg aattgacgga agccaggttc
    52381 tgacccatct gatcacaacc agaaattatc ttcccggatg cagtcttaag gatatgccaa
    52441 acggaagcac cacctacaac ggacttcaga atcccagcca gattaaggga acctggcaga
    52501 gataccagaa caaggaaaca agctgtgatg ttttaacctg ctacggatat ggggacggag
    52561 gcggaggtcc tacagaggaa atgctggagc agagccgcag aatggaaaac ggcgttgtgg
    52621 gtgtaccctg tgtacgtcag acctttgtaa aagacttctt ccatattttg gaaaacaaga
    52681 tggaccgaaa ctgtcttcct tcctggagcg gagaattgta tctggaattt catagaggaa
    52741 catatacctc tcaggcaaaa aataagaagt ataaccggat ctgtgaattt atgatggcag
    52801 atgcagagtt ttattccgtt ttggcaaatg gattgggaaa tggatatgag tatcctgaaa
    52861 aagagctgga aaagaactgg aagcttttgc ttttaaacca gtttcacgac attctgcccg
    52921 gttcatccat aaaggatgtg tatgaggact cagctgtcca gtatgaagaa atcctcagat
    52981 cagccggaag aattgcagaa ggggcaggaa aagccatttt gtcctcatta aaggaaaatg
    53041 gaaataataa agagggattg gctgtcttta atccattaag ctttcgccgg acttctgcag
    53101 cagagctttc ggcagaagag gcccgatggg cagaaaatgg tctgaaaggg ggttgtccgg
    53161 agggaatgat catacagaga acaggggaag gcggtatgct gattctggta aaggatgctc
    53221 cttccaaagg ccttggagta tatgaaaaca gtgtattcga tatacaggca gaggagccgg
    53281 tgatccgcag tcttgtaacc gatgaacggg gctggcccat cagtctggat acaccctttt
    53341 tccatatgga atttgaccca cagggagaga tcagctgcat acgggatctg agagaatcca
    53401 gagagctgtt aaaaaaaggt gctgtgggaa atgagcttat tgtatatgag gaccgtccca
    53461 tggagtttga tgcctggaat attgacgctg gatacagaga aaagaaatgg aaattcggag
    53521 gggttacgga gttttacctg gaagaaaacg ggcctgtgag aggcagcctt ttgatccgcc
    53581 gtcattttct ggattctgtt ttagagcaga ggatctgttt ttattcccat accgccagga
    53641 tcgattttaa gacaaaggca gactggagag agagccagct tcttttaaga acttcgttcc
    53701 cccttgatat acagagcaat gaggcagatt ttgagattca gtttggaaat gtgaagcgtc
    53761 cggttcatca aaatacgacc tgggatcagg cacgttttga ggtatgcgcc cataagtgga
    53821 tggatctgtc cgagtatggg tacggagccg ctgttttaaa tgactgtaaa tatggatgtg
    53881 acatccatga ttccgtcatg agcctgactc tgatcaaatc cggaattttc ccggatcccc
    53941 aggcagatca gggactgcat gagtttacat attccctcta tccccacagg ggagatttca
    54001 gaagaggacg agtgattcag gaggcgtacg acttaaattg tcctctgacc gtacagaaac
    54061 agagcggaat caaaaaggga gaatggtcct tccttcagat ttccgaggaa aatatttttg
    54121 cagatacagt aaagaaggca gaagagggcg atgacctgat cattcggctg tatgaggcat
    54181 atggaatgcg taccagggta catctggtat ttccgttatt ttctgatttt gatgcggcag
    54241 tctgtgatct gatggagcag acggatcaaa agacacagtc ctttgtttca gagacagaac
    54301 tggtgcagaa gggacagact ctggagtttg agatgaagcc atatgaaatt ttctctattc
    54361 gccttacacc aaagtgctgc aggtaaaacc aggattataa gagcaaagat agaactaaaa
    54421 agcaatatat acaatattat atatcaaaaa aggcaaaaac agtgttgaaa ttgatggatt
    54481 aaaatgatac tatgggttta tcaaaagcag ttacgaggaa atgcaaaaac aagaagtatt
    54541 aaggaggaca ttattatgaa gaagaaagca gcaagagcat tggcaggcgt tcttaccttg
    54601 tctatggcag cagcgctgac agcctgcggc ggttctgatt ccgcaaaaaa aggaggagag
    54661 acagcagcgg cagcagaaag ttcaggccct atcacaggac agactctttc tgttatgctt
    54721 tctgaggagc cgggagagca ggatgctctt ggaaacgttt taaaccattg ggcggaggaa
    54781 agcggaaaca cgatcaaaaa gatcattatt tcccatgatg atatgagaag caagttcccg
    54841 gctatggcta agaataaaga ccttccggac attgtttcca ctactttcct tcatcagatc
    54901 tatccggatg agttcgtaaa catggaagat gtggttgatc tctccaaatt taaccagtct
    54961 gcgctgaata tcgtaggcaa gtcctattct tccgacgcga ttacaggtat tacagagcag
    55021 tttacaacga cctgtgtctt ctataataag gatgcttttg ctgcagcagg cattgaagtt
    55081 cctacaccgg aaaatccatg gacctgggag cagctgtatg agaatgccgc agtgcttcag
    55141 gaaaagggcg gcgtaaagta tggctttgca gctgacgtat caagagcacg ttacgatatc
    55201 atgatgtatg caaacggcgg ttctctggta gaaaaggatg gggacagctt tagggtaacc
    55261 gtaaacagcc ctgaaaacat tgcaaccctg gagcgttttg tgcaggcgaa caacgaagta
    55321 atgccaaagg caatctgggc aggcggcacc acagataatc cggttgagta cttcaagaac
    55381 ggagatgcag cgatcctgtt atccggttcc tggaactaca acaccttcac aaatgaaatt
    55441 tccaagtttg atttcggcgt aatgccttct ccaaagggaa gcgagtgcca gtcttccatc
    55501 atcggcggcg cagctcttgc aattcctcag aacggaaaga attcggagct ggcaaagcag
    55561 ttcgtacagt ggctgtttga agaggaacac tacaaggagt acctgcagct tcacaagggt
    55621 ctgtctgtta tgaacgatgt tgcttatgag cctgagactg acaaggcaaa ggaagacttt
    55681 gcgctgatgc agggcgagac cgcttatgta accgatacat ttatgacaga tgagagttct
    55741 tcctggagaa attacaagga taatgagtac agagatgcat taaagcgcgc tgtatccgga
    55801 gagatcacag caaaagaggc tctggacgga tttgcaaagc agctgtctga ggcttccaac
    55861 tggagtatgg catactaatt ataccagacg aaaaacggtc cattcttttt tggggaatgg
    55921 gctgtttttt tgttgcagag aggatggatt gctggtagaa taaaaaagca aaaacctgca
    55981 cataaagcaa aatgtgcgat attattatac ctaaaagaca aaataaatgt agaaattgac
    56041 ttatcaaagt gttaagataa atccagaaag tgatggaagc gaggaacgcc ttccgatgaa
    56101 aggagcgaga ggatgaaata cacagcagca gatattgacc gttacattga tctgtatctt
    56161 aacagctttg aaaaggttct ttacgaagag gattcccagt ttatggaggg gatgaagaca
    56221 cggagtctgg ccggcgatga tatcaggcgg tatcagtttt gggagtggac tcagggcgta
    56281 ggcctttatg gagtatggaa actctacaag gagacgaaag atgagagata tatgaatatc
    56341 ctgacctctt attatgagag gcagttaaaa atcgggcttc cttccaagaa tgtaaatacg
    56401 gtgacgcctc ttttagccat gtcttttgtg gcagaggaaa cagacaacga gcgatacatg
    56461 gaggtctgcc gggaatgggc agcatggatc tatgcagatt ttccaaggac aaaagagggc
    56521 ggccttcagc atattacttc agatacctta aatgagggtg aactgtggga tgacacatta
    56581 tttatgacgg ttctgttcct ggcaaatatg ggccggatcc tgggagaaaa gaaatatcag
    56641 gatgaggcag aatatcagtt cctgcttcat atcaaatatc tcacggaccg caaaacaggc
    56701 ctctggtatc acggctggtc ctttaaggaa aagagccatt ttgcagcagc cttctggggc
    56761 agaggaaact gctggatcac agcggcgatc ccggagtttt tagatctttg tccctgcagt
    56821 ccaagcgtgg aaaagttctt aaaagaaacc tggaagcgcc aggtagaagc tctgatccag
    56881 tatcaggagc ctacaggaat gtggcatacg ctcattgatg ataagacctc ttatgtagag
    56941 tccagtgcta cctgtggatt tgcgtatggc attttaaagg gggctgaact gggactggca
    57001 gaaacagact gggtggaagc tgctcttaag gctcttgaac ccattatgga atacaccgat
    57061 gagactggcg ttgtaaacca ggtatcctac ggaacaccta tgggacgtga aacaaaggat
    57121 ttctacaaag agattgagct taagccaatg ccatacggac aggctctggc catgcttttc
    57181 tttatggaat gtaggaagca tataagtgat ttttaaggaa ggatcaagga ggaaatcgat
    57241 atgaaaaaga gtagaaggca gtcattctgc catacgcaca gcttttggct gttcgtactt
    57301 ccatcgctgg cattatttgc agtatttttc ttggtgcctc tggttttaag catctggttt
    57361 tcattcacta attatgatgg ctggaagacc atggatgtaa ttggtttaaa gaactataca
    57421 gatattttaa aggatggaga tttttataag acagtaggac ggactctttt atatacagtc
    57481 tgcaacctgc cctttaagat tgctattccc ctgcttttag cagcacttgt tacctccaga
    57541 ttggtaaagg gaaagacact gatgcgtacc ctg
//
LOCUS       DBMDUH010000004        49377 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_659_length_49377_cov_5.895426, whole genome shotgun sequence.
ACCESSION   DBMDUH010000004 DBMDUH010000000
VERSION     DBMDUH010000004.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 49377)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 49377)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49377
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_659_length_49377_cov_5.895426"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="ACZPCG_00960"
     CDS             <1..474
                     /locus_tag="ACZPCG_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009245189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2399 domain-containing protein"
                     /protein_id="HIR1283675.1"
                     /translation="LKEKEPVRLTLRTLGELGKVTAQKREVYVVENPAVFSILTSRNP
                     SCAAVCINGQPRLASFLLLDFLRETHQFLYAGDFDPEGLLIAQRLKERYEDSLYLWKY
                     EAEWYKKYLSGVRLSGSRIKKLDHVYSPELLEIKKWMQKEQKAAYQEAMIEIYLD"
     gene            complement(529..1917)
                     /gene="mgtE"
                     /locus_tag="ACZPCG_00965"
     CDS             complement(529..1917)
                     /gene="mgtE"
                     /locus_tag="ACZPCG_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006861491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HIR1283676.1"
                     /translation="MAANTTAAGYSLEIAELIRSNLTPKRMQEQLSSYHENDIALALD
                     LLNKTERTKLYQILDSEALADILEYSDHIAEYVQELSIRKKVDVFSRMETSDVVDVLR
                     HLEKGERETLIDLLDPEVRSDVKLLSSFDEDEIGSVMSTNYIVVSDHASVKEAMSELV
                     RQAAENDNISTIYVVDQYNIFYGAIDLKDLIIAREGTDLKGIMTCSYPYIYARASIED
                     CIPQLRDYSEDSIPVLDNDNKLIGVITASDLVEVVGDELNEDYAKFAGLSAEEDLEEP
                     IFLSVKKRLPWLFILLGMGLGVSATVGLFESIVAQLPVIMCFQSLILDMAGNVGTQSL
                     AVAIRVLMDKQLSGKQKLALIIKESRVGLTNGLILGALSFLIIGGYLVLKGNTVPFAF
                     AVSGCLGTAMVLAMLISSLSGTIIPIFFQKIGVDPAVASGPLITTVNDLVAVISYYGL
                     SWIILINIMQLP"
     gene            complement(2239..3090)
                     /locus_tag="ACZPCG_00970"
     CDS             complement(2239..3090)
                     /locus_tag="ACZPCG_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR1283677.1"
                     /translation="MEQKHYLYSSGEFAKRAGVNKRTLHYYNDIGLFCPEVIEENGYH
                     YYTCFQFAQLEMILTLRKIGLSIEEIREYASSPSDDSFSRIMEKRKLWIEESIQQLLS
                     EQSFLEQKAERLKIAMNAKHGSIQLCSLPQQKILISSAITGKYDEADFSVAADFSLRL
                     KETFHLYDSFGSRISMDSIRRKHFNSYSSFFAYCPHTSEAYDEILPAGTYLRSFCIGT
                     WDKLPQVYRSILSYAKEHHLLLTGYAYEEGLNEMALKNQADYITMITIPCSVSDLTEK
                     RLCFPEK"
     gene            3172..3711
                     /locus_tag="ACZPCG_00975"
     CDS             3172..3711
                     /locus_tag="ACZPCG_00975"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1283678.1"
                     /translation="MEFTSVKKQQWSEIKEIYMEAFPKCERKPFFLLRQSIKRKKAVL
                     AVAAEGNQLLGFTVFVVYKDMVMIDYLAVSSKIRSRGTGSRILEHICGQFPDKKIVLL
                     IERVDDMAENCQQRTARRRFYLKNGFTSSGIFITGAGGDMEVLNYGGTVSQEEYMDLQ
                     RYALGKLFFRLSRIQLAEV"
     gene            3932..4966
                     /locus_tag="ACZPCG_00980"
     CDS             3932..4966
                     /locus_tag="ACZPCG_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005423983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HIR1283679.1"
                     /translation="MEPYYYTKLPKHHQSVYHAIFQGLMNLEDSFLVPRIDGRELTDL
                     FFRLRLDHPEIFWASGFHYKYYQDSPNLIFEPEYLFEKGKIKEHKKALASRVEKLIRP
                     AAKLSEWEKEKYIHDFICENVRYDKLKKAYSHEIIGPLGQGVGVCEGIAKAVKVLCDG
                     LGIWCMIAICGNNPEKGIKYRHTWNIVQIGGVYYHLDTTFDNTLGKHNCAEGGIRYDY
                     FNLDDKAVFRDHEPLIAPAPSCTDGDHFYYKEKKLSFTKAEDVYKRACQAAKKGKPLT
                     FHWRGGYLTRAVLEELLDLMKKAGEEREKTARIALNWPQAVIRLTYADGRAESSVSVD
                     EANEGEQEGT"
     gene            4972..6828
                     /gene="recQ"
                     /locus_tag="ACZPCG_00985"
     CDS             4972..6828
                     /gene="recQ"
                     /locus_tag="ACZPCG_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018594710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HIR1283680.1"
                     /translation="MTQQEILKHYFGYDSFREGQERLIEAILEGRDVLGIMPTGAGKS
                     LCYQIPALMMDGITLVISPLISLMKDQVSNLNQAGILAAYLNSSLTYGQYRKALEFAR
                     AGRYPIIYVAPERLMTEEFLQFALDPQVKIAMVAVDEAHCVSQWGQDFRPSYLKIVDF
                     IRQLPVRPVVSAFTATATAEVRDDIMDILMLRQPEILTTGFDRGNLYFGVLSPKDKYA
                     ALVNYLERHKGESGIIYCITRKVVEEVCANLIREGFPVTRYHAGLGDEERRHNQEEFI
                     YDHSPIMVATNAFGMGIDKSNVRFVVHYNMPKNMESYYQEAGRAGRDGEPAECILLYG
                     GQDVITNQFFIDHNQENDALDPMTRQIVMERDRERLRKMTFYCFTNDCLRDYILRYFG
                     EYGSNYCGNCSNCLTQFETVDVTEIAETLIRCVQTSGQRYGTNVIIDTVHGANTAKIR
                     NYRMDGNICYGKLAKVPVYRLRQVINHLQLSGFLTVTNDEYAIVHLTESSGRILAGEE
                     TVMMKMAKEQEHTKTDTGSRKKKAKGVLAGMAEADMPLFERLRELRAQTAREEKVPPY
                     IVFSDKTLAHMCVLKPEDREEMLSVSGVGEFKYEKYGERFLACIREWKEKEA"
     gene            6830..7987
                     /gene="rsmH"
                     /locus_tag="ACZPCG_00990"
     CDS             6830..7987
                     /gene="rsmH"
                     /locus_tag="ACZPCG_00990"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HIR1283681.1"
                     /translation="MDQDKRKEIINSSETQEDLGVREDGVQVTGVQESGIQSESAVPH
                     KRRVRYKGTHPRKFEEKYKEHQPEKYRDTIEKVISKGSTPAGMHISIMVNEILDFLQI
                     QPGQTGLDATLGYGGHTGHMLEQLKGQGHMWALDVDTIEMEKTRKRLADRGFGPDILT
                     IKHLNFANIDQVAEEAGGFDFILADLGVSSMQIDNPERGFSYKTDGPLDLRLDSSRGM
                     TAAERLKELDEEELVGMLVENSDEPYAKEIARRVMRELRNGNAVDTTTRLRELIEEAL
                     VFLPVADRKEAVKKSCQRCFQALRIDINSEFEVLYSFLDKLPGALKPGGRAAILTFHS
                     GEDRLVKRAFKDGKKAGLYSEISADVIRPSAEECIKNGRARSTKLRWAIRG"
     gene            8056..9384
                     /locus_tag="ACZPCG_00995"
     CDS             8056..9384
                     /locus_tag="ACZPCG_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015574543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HIR1283682.1"
                     /translation="MEQLSLFDTEAVYDPLASRMRPEDLDEFAGQTNLLGKGKLLRQL
                     IEQDRIPSMIFWGPPGVGKTTLAGIIAKRTHAEFVNFSAVTSGIKEIREVMAQAEGRR
                     RMGGKTVLFVDEIHRFNKAQQDAFLPFVEKGSIILIGATTENPSFEINSALLSRCRVF
                     VLHSLSNEELVQLIRRALTSPKGLGYLKVDISSEMIEKIAAFANGDARTALNVLEMAV
                     ANGEITGEKTIVTMDTLMQCIGKKSLLYDKKGEEHYNLISALHKSMRNSDPDAAVYWL
                     ARMLEAGEDPLYVARRLVRFASEDIGMADSSALPLAVAAYQACHFLGMPECNVHLSHA
                     VIYLSTAPKSNSSYMAYEAAKEDAKEMLAEPVPLVIRNAPTDLMKELHYGDGYVYAHD
                     TKEKIVRMQCLPDSVKNRVYYRPGIQGDEKVIKDRMERIKQWRNKGVSEH"
     gene            complement(9381..9821)
                     /locus_tag="ACZPCG_01000"
     CDS             complement(9381..9821)
                     /locus_tag="ACZPCG_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR1283683.1"
                     /translation="MNGNTDIRDLMIQIEKARKRLVQPFLQNLGLTLGQGHARILNCL
                     LEEDHLTQTELAGRCRMDTTTMSRSLDKLEKSGYLVRERDPDCRRSFLICLTKAGREE
                     AAQVHQGFDRLNDVICEGMDPEEQELLKKNLERILKNLKECPEL"
     gene            complement(9840..11732)
                     /locus_tag="ACZPCG_01005"
     CDS             complement(9840..11732)
                     /locus_tag="ACZPCG_01005"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1283684.1"
                     /translation="MARIANFSRPKNTRRAIKQMFGYLGRHKWYMFAIALLVAASAMA
                     NIMGTYLLKPVINNYILPGDIPGLIRMIALMGLMYALGASACLAYNQLMLHMSQQVVS
                     EIRSDLFEHTQKLPLSYFDAHTHGELMSRFTNDVDTLTEALNGSFAMMIQSFITITGT
                     ITMLLILSWRLSLIVIFFLACMTLFIRYNSRRNRKYFIRQQQCLGSINGFVEEMVAGQ
                     KVEKVFNHEPQDFEEFCRRNEALRDAGTKALAYSGMTIPTIVSLSYFNYAVSACVGGL
                     FALAGLTDLGTLASYLVYVRQSAMPMNQFTQQINFLLSALSGAERIFDMMDEIPEIDE
                     GNVRLCQVRENPMGQLKECSEFTGRFAWKVPEETVKRTRLDAPVSDGFAFIPLKGDVR
                     FNDVVFGYVPEKTILNGISLYAKPGQKIAFVGSTGAGKTTIINLVNRFYEVNGGSITY
                     DGIDIREIRKDDLRRSMSMVIQDTHLFTGTIADNIRYGRLDASDEDVRAAAQVANAHS
                     FIRRLPQGYDTVLHSDGANLSQGQRQLLAIARAAISRPPVLILDEATSSIDTRTEKLI
                     EQGMDALMEGRTVFVIAHRLSTVRRSEAIIVLEHGEIMERGDHDELLEQKGRYYQLYT
                     GQFELD"
     gene            complement(11732..13456)
                     /locus_tag="ACZPCG_01010"
     CDS             complement(11732..13456)
                     /locus_tag="ACZPCG_01010"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1283685.1"
                     /translation="MKEFMSYLRPYRRDGILAAFCIVTETIFELVIPLVMASIVDVGV
                     ANGDRRYILAKGAQMILFALIALILGQGSAIFSARFGHGLGAELRRAEFARLQEFSFA
                     NTDHFTTSSLVTRLTGDVTAIQNSVATGLRPGLRSPIMMLTAMTASFLINPRLAMVFL
                     VAAPILGIMLFFIISHVRPLYSRMQGAMDQVNRIIQENLTAIRVVKSYVRGDYETAKF
                     EQVNTELRTASEQAFRLAALNMPAMQFVMYGTILSILWFGGNILTIGGIGVGELTGFL
                     SYVLQVLNSLMMFSNVFLMITRAMASWHRIMEVMHEDIDIKEDRATDIRVERGEIEFN
                     HVSFKYNKESSEDVLSDISLHIKAGQTVGIIGQTGAAKSTLVQLIPRLYEATKGTVLV
                     DGHPVWEYPLRELRDSIAVVLQKNTLFSGTVKDNLRWGKETATDEEIDAACRIACADE
                     FIGRLAKGYETELGQGGVNVSGGQKQRLCIARAILKAPKVLILDDSTSAVDTATEAKI
                     REGLAKSLPDTTKIIIAQRISSVLHADQIFILEDGRLAGCGTHEELLKHNSIYQEIYY
                     SQQEGANL"
     gene            13682..16564
                     /locus_tag="ACZPCG_01015"
     CDS             13682..16564
                     /locus_tag="ACZPCG_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIR1283686.1"
                     /translation="MKDKIIHLIASEDSRNPMTDEEIAETLGIFRETVTNIRKEAGLG
                     DSRERRKAAVFQDMKELLNMDPDLTERGLTRCLAERGYVIGKYAVGKLKEELLKEWQP
                     EPEYGKTGEEIQDGSRFFSKVIGYAGSMKSQIKQVQAAILYPPKGLHCLLYGPSGVGK
                     SYLAEIMHEYAVTTDNFASDAPYFAFNCADYADNPQLLLSQLFGYSKGAFTGASENKK
                     GIVEQCDGGILFLDEVHRLPPEGQEILFYLMDKGKFRRLGETEAQRESHLMIIAATTE
                     DPRSSLLLTFRRRIPMMIEIPSLKERPLKEKLQFIRLFFWIESRRLNAPVLVRAEVIR
                     YMADEEYQGNVGQLKADIQVCCARAFLDGRTKRRSKMIVGMEALSGNKTEPISSGEHS
                     RLAELVPEDEIFTPDMPAVTTAETDNGWDIYRDLEERYSALTKEGLKENEIEEKLLRE
                     IESSFQLHIRQVEEASLSLEELSHITGSDVMEMAKEVYELASQRLPYLKREMIIPLAI
                     HLKMACERLKQQERIVEKGIKNLGELYRDEYEAAKEILNYICKKYYVCFYKEEAGFLA
                     MYFHRFQEKHRKEGCVGVAVVSHGPVASAMAEVANSIMGVSHAVGVDMGIKDTPVQMA
                     ERTMEAVRRIDQGKGVLILADMGSLLDMEEKVKKETGITVKIVGRTDTMMVIEAIRRT
                     YWTDESLETIAKVLDWKQFSPAKRQTRKPREKAVLCLCITGEGAARMLKSYLEERLKS
                     TLGNVRIITKGYIENTDITELIRASEEEYEILAIAGTIDPKQTQYPFLSVSEIYAKEG
                     IRTLRQILKRHFFKEQNCLQDVIFRDLIFIQEDFAYKEQILDQCVEAMAEKGLVQSEF
                     LLSVYKREGMMPTFLKGGIAIPHGAENLVTKPVICITKLDKPIVWDGFNTVDLVCVLA
                     LNEKSTKYFEQFYQVISDEIMVSRIRSCHTPDEIYGILCNYTKTVK"
     gene            16622..17263
                     /locus_tag="ACZPCG_01020"
     CDS             16622..17263
                     /locus_tag="ACZPCG_01020"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - (R,S)-4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HIR1283687.1"
                     /translation="MKVNDFPKVTVILRGYTYDQIHTVVKNLIGTELKAVEITMNTPG
                     ALETIRKIREEFGDSILVGAGTVLTYTEAQQAIDAGAAFLLSPTVFDKEILDLCRERG
                     VISVPGAFSPSEIWKSIQDGADIVKVFPAGRLGSRFFSDIQAPLGRLPLMAVGGVNVE
                     NLEEFFNAGASFAGIASGIFNRQDIIEQREEHLRTSIREAEKKIRQLREAQEE"
     gene            17265..17741
                     /locus_tag="ACZPCG_01025"
     CDS             17265..17741
                     /locus_tag="ACZPCG_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIR1283688.1"
                     /translation="MSAIIFDESFILRFDTHLTNTEVLSAMADNLCKRGIVKETYKKA
                     ILDREQEFPTGLNTGGINVAIPHADISHVNKAAICVGILKEPAAFHAMDEPDYDVPVK
                     LVIMLALTEAHGHIEMLQKIVGLIQDQAAVKEITETDDSEKAGELISRFLLEEKEN"
     gene            17745..18023
                     /locus_tag="ACZPCG_01030"
     CDS             17745..18023
                     /locus_tag="ACZPCG_01030"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HIR1283689.1"
                     /translation="MKRILVACGNGIATSTVVATKIREKCEENGIAVTVTQCKLLEVN
                     AKADNFDLLVTTGKFTGGNVNIPVVGAISLLTGIGEEETLDQILDYVR"
     gene            18070..19431
                     /locus_tag="ACZPCG_01035"
     CDS             18070..19431
                     /locus_tag="ACZPCG_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HIR1283690.1"
                     /translation="MQIIYNIFNVILDAGAVVMLPIIITIVGLIFQVKLSKAFRSGLT
                     IGIGFAGINLVINMLKSNLGPAAQAMVENFGIKLDILDVGWGAIAAVTWSSPIIAILI
                     FCILATNIIMLALKATDTLDVDIWNYHHMAIVGIMVYFVTKNVALGIAATVVMAIVTF
                     KMSDWTAPLVEEYFGIPGVSLPTMSALSSIVIAAPLNWILDRIPGINKIDFEIKDAQK
                     YLGFFGEPTMMGLILGCIIGVLAKYNLASILSLGVNMAAVMVLIPKMTSLFMEGLMPI
                     SAAAKNFTQKKFKGRKFLIGLDAAVVVGNPDVITTSLIVIPMTILMAAVLPGNRVLPF
                     ADLAVVTFRVALVVAVTRGNLFKNIIMGLVCTGAILLAGTATAPVLTELALSVGLDLG
                     EGLITSFAATSLTVSFLVYKAFISNLYISVPLLVLVVGGTWMVMSRINKKKAEMAAQG
                     EQA"
     gene            19464..21101
                     /gene="ilvD"
                     /locus_tag="ACZPCG_01040"
     CDS             19464..21101
                     /gene="ilvD"
                     /locus_tag="ACZPCG_01040"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003525640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - L-valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HIR1283691.1"
                     /translation="MQRAIDRLEPFQRAISKAHLAAAGVSVDALEKKPLIAIANSWNE
                     ICPGHEPLKKLAEEVKKGILEAGGEPVEFNTIAMCDGIAQGHSGMRYCLPHREIVTDS
                     IEAMVVGEGVFDGVVYMASCDKIIPAMLNAAARIDLPSVIVTAGPCFAQIKPNESKEL
                     RQRFLRGEITERQLIEGTLKYYTGPGVCPFLGTANTMGALCESLGMMLPGSSLIPSST
                     SMRRFSARESGSTVMKLVEQQIRPSQIITKEALENTVTLLSAIGGSLNAMIHLPALAA
                     ELGLELDWDDIAKITSKTPVLCGIVPNGNLTAVDLHYAGGIPAVMKELRDKLHGECLN
                     VCGLSLGEILEQTKDGDRTVIHTAGEPLEKANGIQVLYGNLAPQGALVKTSAVPENVR
                     VFEGTAKVFDGEDACYAAFRDHAIHEGDAVIVRYEGPKGGPGMKELHRITEIIKGIPR
                     TAVITDGRFSGASGGLSIGYLCPEAEEGGPIALVKNGDRIQVDLYKGMIHLDVSDEEL
                     ISRRNAWEHGEKKKEKGLLGRYAAMVGNARQGATFHI"
     gene            21462..22217
                     /locus_tag="ACZPCG_01045"
     CDS             21462..22217
                     /locus_tag="ACZPCG_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR1283692.1"
                     /translation="MMQYLIVCPLVFLAGLVDSIAGGGGLISLPGYMIAGVPAHMALG
                     TNKMSSTIGTLVSTARLLKHGYLKGKIAVAAVSAVFAMTGSVLGAQIALRVPDSVIRS
                     MMIVILPIVAYYVLRNKNMGDSERTGTISKRRVCVISLAASFVIGTYDGFYGPGTGTF
                     LILVLTGAARMDMRTAAAQTKVINLSSNISALVTFILTGNVYYTLGAAASVCSVAGHY
                     LGAGLVVHDGQKIVRPMVLVVLGILFLKIIAGA"
     gene            22274..23230
                     /gene="prmA"
                     /locus_tag="ACZPCG_01050"
     CDS             22274..23230
                     /gene="prmA"
                     /locus_tag="ACZPCG_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HIR1283693.1"
                     /translation="MKWKKFTLTTTTEAVDLVSSMFDDIGIEGIEIEDNIPLTEKETK
                     GMFIDILPELPPDEGIAKVSFYLDDDADVAEMLKKVNEGLDELALFTDLGARTIEASE
                     TEDKDWINNWKQYFKPFTVDHILIKPTWEPIPEEHKDKLLVQIDPGTAFGTGMHETTQ
                     LCIRQLEKHVHGQAEILDVGTGSGILGITALKLGAKEVWGTDLDENAITAVGENLEAN
                     SISSDSFHVLQGNIIDDQAVKDWAGYEKYDIVVANILADVIILLVDEIPAHLKKGGYF
                     ITSGIINMKEEAVKAAFAANQELEVTEITYQGEWVSVTARKK"
     gene            23234..23983
                     /locus_tag="ACZPCG_01055"
     CDS             23234..23983
                     /locus_tag="ACZPCG_01055"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HIR1283694.1"
                     /translation="MHHFFADPTQVAEDTIFITGPDVNHMKHVLRMKPGEEVLISDGT
                     GRDYTCEVEAFEPDRAVVRIHSVCDSRMELPAEIWLFQGLPKADKMELIIQKAVELGA
                     SAVVPVATKNAVVRLDEKKAQAKKKRWQAIAESAAKQSKRSRIPQVELVMTLKEAFSF
                     VHDREFGLCLIPYEQEKGMDAMKQALEKTAPGQKIAVFIGPEGGFDEGEIRQALEMGV
                     CPVSLGSRILRTETAGLAILSVLMMKLEGAF"
     gene            23999..25153
                     /locus_tag="ACZPCG_01060"
     CDS             23999..25153
                     /locus_tag="ACZPCG_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002571822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HIR1283695.1"
                     /translation="MEVYFDNSATTRVLDEVKDLTVKLMTEDYGNPSARHRKGMEAEQ
                     YVKNAASQIAKTLKVKEKEILFTSGGTESNNMALIGTALANQRAGKHIISTRIEHASV
                     YQPLAFLESLGFEVTYLSVDSEGHISLEELEQSIRPDTILVSVMYVNNEIGAIEPVEE
                     IASLVHGKGKNIIFHVDAIQAYGKIVIRPKKQGIDLLSVSGHKIHGPKGVGFLYIDEK
                     VKIKPLLYGGGQQRDLRSGTENVPGIAGLGLAAQEMYRDHEAKIGKITGLKDYFIDRA
                     LELEDVTVNSQKGGLSAPQIVSLSFKGVRSEVLLHALEDKEIYVSSGSACSSNHPAIS
                     GTLKAVGVKKELLDSTLRFSFGLFNTKEEIDYCMDVLKELLPVLRRYYRA"
     gene            25189..26370
                     /gene="thiI"
                     /locus_tag="ACZPCG_01065"
     CDS             25189..26370
                     /gene="thiI"
                     /locus_tag="ACZPCG_01065"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005331688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HIR1283696.1"
                     /translation="MQYQSFLIKYAEIGTKGKNRFMFEDALIKQIRFALREVDGSFEV
                     TKESGRIYVTAEGDYDYDDTIEALKRVFGIADICPMVQIDDRDYENLKKHVVEYMDKV
                     YPDKNLTFKVVARRGDKRYPVSSDQINRDMGEAILEAFPQMRVDVHNPDVLLRVEIRQ
                     KVNIFSLMIPGPGGMPVGTNGKAMLLLSGGIDSPVAGYMIAKRGVKIDAVYFHAPPYT
                     SDRAKQKVVDLAKLVARYSGPMDLHVVNFTDIQLYIYEKCPHEELTIIMRRYMMRIAQ
                     AIAEKNGAIGLITGESIGQVASQTLQSLAATNEVCTMPVYRPVIGFDKQEIVDVSEKI
                     GTYETSIQPFEDCCTIFVAKHPVTKPNINVIRNSERHLEEKIDELVKTALETTEVIRC
                     Q"
     gene            complement(26434..26667)
                     /locus_tag="ACZPCG_01070"
     CDS             complement(26434..26667)
                     /locus_tag="ACZPCG_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HIR1283697.1"
                     /translation="MKTVRISLNSIDKVKSFVNDLAKFDVDFDLVSGRYVIDAKSIMG
                     IFSLDLSKPIDLNIHAENSVEDILSTLAPYIID"
     gene            26806..28179
                     /gene="mtaB"
                     /locus_tag="ACZPCG_01075"
     CDS             26806..28179
                     /gene="mtaB"
                     /locus_tag="ACZPCG_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003524464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035598 - tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HIR1283698.1"
                     /translation="MPKAAFHNLGCKVNAYETEAMQQQLAQRGYEIVPFDEKADVYII
                     NTCSVTNIADRKSRQMLHRAKKLNPQALVVAAGCYVQVASEELKKDASVDLIIGNNRK
                     AELADILDARLAEKNEENRVYVLDIAHTHEYERLHLDRLGGHTRAFIKVQDGCNQFCS
                     YCIIPYARGRVRSRSHEDVAEEVKGLVAGGYKEVVLTGIHLSSYGIERMEENPVSHGD
                     WNHRELLALIKEVHAIEGLERIRLGSLEPRIITREFAEELAKLPKFCPHFHLSLQSGC
                     DETLKRMNRHYTTEDYEARCRLLREVFDHPAITTDVIVGFPGETEEEFALTKEYLERI
                     HFYEMHIFKYSRREGTKAAVMKDQIPESVKAERSDVLLSLETKMSEEYRKHYGDSVLS
                     VLFEEITVIDGKEYLTGYTPQYVRAALPVTDREICSRMAGNIFEVQAEGFVTDEALRV
                     KLQEITV"
     gene            28274..28534
                     /locus_tag="ACZPCG_01080"
     CDS             28274..28534
                     /locus_tag="ACZPCG_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007708776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HIR1283699.1"
                     /translation="MGNIRETQYFQAVQDKKMEVSDVLEQVYIALTEKGYNPVNQIVG
                     YIMSGDPTYITSHKGARSLIMKVERDEILEELMAVYIDARLK"
     gene            28843..29313
                     /gene="ruvX"
                     /locus_tag="ACZPCG_01085"
     CDS             28843..29313
                     /gene="ruvX"
                     /locus_tag="ACZPCG_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007708777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HIR1283700.1"
                     /translation="MRIMGLDYGSKTVGVAVCDPLGITAQTVETITRKDENKLRQTLA
                     RIEALALEYGVERIVLGYPKNMNNTLGDRAKKSEELKEALERRTGLPVVLWDERLTTV
                     AAERVLMESGVRREHRKESVDQIAAAMILQGYLDRMAMEQKKEETAGDMPESGE"
     gene            29315..29593
                     /locus_tag="ACZPCG_01090"
     CDS             29315..29593
                     /locus_tag="ACZPCG_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HIR1283701.1"
                     /translation="MEERTDCITMVTEDGDEVVFSVLEETRIHGKDYLLVTDAPEDED
                     GECYILKDVSGEQEPDAVYEFVEDDRELDSVMGIFEALLSDADVEIEK"
     gene            30035..31669
                     /locus_tag="ACZPCG_01095"
     CDS             30035..31669
                     /locus_tag="ACZPCG_01095"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012200309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0004534 - 5'-3' RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HIR1283702.1"
                     /translation="MIPLGGLEQIGMNITAFEYEDSIIVVDCGLAFPGDDMLGIDLVI
                     PDVTYLKQNIDRVKGFVITHGHEDHIGALPYILQQVNVPVYGTKLTIALIEHKLKEHN
                     LLKTTRRRVMKHGQSVNLGCFRVEFIKTNHSIQDASALAIFSPVGIVLHTGDFKIDYT
                     PVFGDPIDLQRFAELGKKGVLAMMADSTNATRPGFTMSERSVGKTFDAIFAEHTNHRI
                     IVATFASNVDRVQQVVNTAYKYGKKVVVEGRSMVTIIDIASELGYINIPDGTLIDIDQ
                     LKNYPPESTVLITTGSQGESMAALSRMAASIHKKVSIMPGDVVVLSSSPIPGNEKAVA
                     NVINELSTKGAKVICQDTHVSGHACQEDLKLIYSLVHPKFAIPVHGEFRHRVAQKELA
                     EYMGVAKDNAILVNSGDVVALDEDSCQVIDHVTAGGILVDGLGVGDVGNIVLRDRQNL
                     AQNGIIVVVLTLEKHSNQLLSGPDIVSRGFVYVRESEDLLEEAHNIVANAVEDCLDRR
                     VSDWGKIKNIIKDSLSEFLWKRMKRNPMILPIIMEV"
     gene            31703..>32189
                     /locus_tag="ACZPCG_01100"
     CDS             31703..>32189
                     /locus_tag="ACZPCG_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HIR1283703.1"
                     /translation="MSRRTREINRITTAIITISIKLMVLALLILLLYEAVVRGFAFGH
                     EIFYAEAAEAAPGREITVEIKEGETAKEAAGELKKKGLITNDFAFWFQSIFYDYQDIY
                     PGIYTLNTSMTSKEILRELNVKPEEESEASPEARTVSGEADLSKDADSSENRKTRAVQ
                     AE"
     assembly_gap    32190..32289
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            32349..33002
                     /locus_tag="ACZPCG_01105"
     CDS             32349..33002
                     /locus_tag="ACZPCG_01105"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008718492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HIR1283704.1"
                     /translation="MIVNDRITDYIQSLETSQGPVLDEIEKEALDQFVPIIRKETAAF
                     LRTMTAALKPSAILEIGTAVGYSALQMCRVMPEHCRITTIEKYEKRLPIARENFHRAG
                     ESGRITLLEGDADVHLKELAKKGCQFDLVFMDAAKGQYLNWLPMVLDLMPKGAVLISD
                     NVLQDGDIIQSRFAVERRNRTIHSRMREYLYELKHTEELETSVIPIGDGVTVSTKVR"
     gene            33012..34238
                     /locus_tag="ACZPCG_01110"
     CDS             33012..34238
                     /locus_tag="ACZPCG_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007708786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HIR1283705.1"
                     /translation="MRETELLIPAGSLDVLKTAVVYGADAVYIGGEVFGLRAKAHNFS
                     KEEMKEGIAFAHERGVKVYVTANILAHNDDLPGVEAYFEELKEVGPDALIISDPGIFA
                     IAKRVLPQMELHVSTQANNTNYGTYLFWHQLGAKRVVSARELSLEEIKEIRAHIPEDM
                     EIESFIHGAMCISYSGRCLLSNFFTGRDANQGACTHPCRWKYSIVEETRPGEYMPVYE
                     NERGTYIFNSRDLCMIEHIPELIDAGVDSFKIEGRMKTALYVATVARTYRKAIDDYKK
                     DPALYEQNMEWYKEEIGKCTYREFTTGFYFGKPGADAQIYDSNTYVKNYTYLGTVEES
                     EGCRFRIEQKNKFSVGETIEIMKPDGRNVQALVKSICDEEGNAQESAPHPKQILWVEL
                     DAPADVYDILRKKAEQ"
     gene            34258..35082
                     /locus_tag="ACZPCG_01115"
     CDS             34258..35082
                     /locus_tag="ACZPCG_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002593285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HIR1283706.1"
                     /translation="MVDGILLILAALCAVYFVIIVIYGGIGTSFAFIWLFFAALCVFL
                     VYGKWYYSRHMFDIPKWVPVSIVTTCVMGVTVMAMICTLVFLGVASEEEPGLDYVIVL
                     GAKVKEHTVSNSLKKRLDKAIDYAQKNPETYLVLSGGKGADEPISEAQAMYKYLVYNG
                     VRPEQLLLEDRSASTVENIAYSRLVIEGHRERLRLEREQQEKKRREPYLMVDDKPVEI
                     GVLTSNFHVFRARMIARKWGFEHVYGISAQSDPVLFIHLCVRECASIIKDRLMGNM"
     gene            35113..38061
                     /locus_tag="ACZPCG_01120"
     CDS             35113..38061
                     /locus_tag="ACZPCG_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003524472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HIR1283707.1"
                     /translation="MIDKKMLDRVKELSQYRLVDERPVEEMNSYGMVLEHKKSGARIF
                     LMSCEDDNKVFSIGFRTPPQDSTGLPHILEHSVLEGSEKFPVKDPFVELVKGSLNTFL
                     NAMTYPDKTVYPVASCNDRDFQNLMDVYLDGVLHPSIYREPKIFLQEGWHYEMEETDS
                     PLIINGVVYNEMKGAFSSPESVLDRYTKAVLFPDTPYRHESGGDPACIPDLTYEQFIA
                     FHKNYYHPANSFIYLYGDMDMAEKLEWLDRAYLSQYDRTDFTLDSRIPMQEPFKEPIE
                     RETTYSVTAEEGTKGRTYLSLNTVVGTDLDPELYVAFQILEYALIDAPGAPLKQALID
                     AHIGQDILGGYDNGILQPCFSVIAKNAEREQKGEFLAVVKGTLRRLADQGIDRKSLLA
                     GLNYYEFRYREADYGSAPKGLMYGLWSMDSWLYDGDPMLHLQYQETFDFLKKAVSEGY
                     FENLIRGYLLDNPHEAFVIVSPCTDQTAREDEALAERLKAYRDSLSEEERSEIVRKTK
                     ELKAYQEEPSSQEDLEKIPMLQRSDIEKKAEGFSYEVKEESGIPVIHSPLFTSGIGYL
                     KVLFDFSVLPDEDIPYGALLKSVLGYVDTEHYSYSDLTSEIYLNSGGLDFSVGGSQKM
                     GEPGNFTGTFTASVKVLYEKLDFAFEMLEEILLRSKLTDEKRLGEILDETMSRARMRL
                     ENSSHSSAAMRAASYYSPLSSFYDRTGGIGFYQFLEKITERCSKEEGYRLKLGKKLME
                     VAKTLFTASNMTVSYTADEEGYGRLPAALAAFKAVLPEGNGIRYPYEFKPELKNEGFR
                     TASQVNYVACCGTFGESAYTGALKVLKVILNYEYLWVNLRVKGGAYGCMSSFGRNGET
                     MLVSYRDPKLGETNRVYEGIPEYLRTFSIDDRDMTKYVIGAFSELDAPLNPAAKGARN
                     LGAWLSGVTDEMIQRERDQVLNVTQEDIRALAGLLESVLNTGALCAIGNDQQIVNEQE
                     LFNEIKHLYH"
     gene            38081..38992
                     /gene="era"
                     /locus_tag="ACZPCG_01125"
     CDS             38081..38992
                     /gene="era"
                     /locus_tag="ACZPCG_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HIR1283708.1"
                     /translation="MEENVQKKSGFVTLIGRPNVGKSTLMNHLIGQKIAITSDKPQTT
                     RNRIQTVYTDERGQIIFLDTPGIHKAKNKLGEYMVDVAEHTLKEVDVILWLVEPTTFI
                     GAGERHIAEQLSNVKTPVILVINKIDTLKKQEEILTFIDAYKDVCKFAEIVPLSALKE
                     KNTDLLLELLFKYLPCGPMFYDEDTVTDQPMRQIAAELIREKALRLLDDEIPHGIAVT
                     IEKMRERKNGIMDIEASIVCERESHKGIIIGKGGSMLKRIGTNARREIENMLEMQVNL
                     QLWVKVRKEWRDSELYMKNYGYDKKDV"
     gene            39007..39657
                     /gene="recO"
                     /locus_tag="ACZPCG_01130"
     CDS             39007..39657
                     /gene="recO"
                     /locus_tag="ACZPCG_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HIR1283709.1"
                     /translation="MREAVSLTGMVLLAASFGEADRRVVLLTRERGKITAFAHGARRP
                     GSALMAVTRPFVFGTFNVFEGRSAYTLQSAQPVCYFDELSADMEGACYGAYFLEMASY
                     LAQENMDGTELLTLVYQTLKALLNPSLPNRLIRRIFELKVMVINGEYTENPQGKVSES
                     CTYTWQYVIYSPVGSLYTFVLKPEVQAEFEHQVENARTTFIRHNFRSLEILELMTR"
     gene            39822..40466
                     /locus_tag="ACZPCG_01135"
     CDS             39822..40466
                     /locus_tag="ACZPCG_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1283710.1"
                     /translation="MKKSIRGILLLAACTVFFAGCARSKGSWEAFTENTLYVAGDGSV
                     SWASVESFGEGDYKEEELKAFAGERISQFNQSQGEEPAFENEKGAEKLPVALDTVSMK
                     DGRAVLVTEYDTPSRLVEFSQDIGDEGMPFDSIDIGSPASLALKEQTSYLDTEGKAAD
                     QQEILKETGLAVRVSGQGVISAEKKIRYISEGCTLRDVHTVETASEGVSCIILE"
     gene            40521..41909
                     /locus_tag="ACZPCG_01140"
     CDS             40521..41909
                     /locus_tag="ACZPCG_01140"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012739580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HIR1283711.1"
                     /translation="MEKTMEKIVALAKNRGFVYPGSEIYGGLANTWDYGNLGVELKNN
                     VKKAWWQKFVQESPYNVGVDCAILMNSQTWVASGHLGGFSDPLMDCKQCKERFRADKL
                     IEDYNDEHGIEIEGSVDGWSQEEMKQYIEDKHICCPSCGAHDFTDIRQFNLMFKTFQG
                     VTEDAKNTVYLRPETAQGIFVNFKNVQRTSRKKVPFGIGQVGKSFRNEITPGNFTFRT
                     REFEQMELEFFCKPGTDLEWFAYWKQFCIDWLQTLGIKPEEMRARDHSPEELCFYSKA
                     TTDLEFLFPFGWGELWGIADRTDYDLTQHQNVSGQDMAYFDDESKEKYIPYVIEPSLG
                     ADRVTLAFLCAAYDEEEIGEGDVRTVLHFHPALAPVKIGVLPLSKKLNEGAEKVFAEL
                     SKYYNCEFDDRGNIGKRYRRQDEIGTPFCVTYDFDSENDQAVTVRDRDTMEQVRVPIA
                     ELKAYFEDKFRF"
     gene            42118..43614
                     /locus_tag="ACZPCG_01145"
     CDS             42118..43614
                     /locus_tag="ACZPCG_01145"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003498065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1283712.1"
                     /translation="MKIRTRLAVAFIIITVFPMLMIYLSVIALSDYQEKSFKEAYGLT
                     EQIELLNGNSLQVFNRLTRQSQKDIRKVLDENPDNYKDPAYLDKVNAKLNEQYAYLIV
                     RREDTIIYYGANGEPQDASLCAQLPDFDVMKGRMEGGIYLDGESQHLIKQMDFQFSDG
                     AEGSVFIVSNVDDLLPEVKSMFREMLFLGVAILVVAGFTLTVWVYRSILSPLNKLQEA
                     TKQIRDGNLEFTLDVDADDEIGQLCQDFEEMRIRLKENAEEKLQYDKESKELISNISH
                     DLKTPITAIKGYVEGILDGVASSPEKLDRYIRTIYNKANDMDRLIDELTFYSKIDTNK
                     IPYTFAKINVSQYFKDCLEEVGLDMEARGIELGYFNYADEDVMVIADAEQMKRVINNI
                     ISNSVKYLDKKKGIINIRIKDVGDFIQIEIEDNGKGIAAKDLPNIFDRFYRTDSSRNS
                     SQGGSGIGLSIVRKIIEDHGGRIWATSKEGIGTEIHFVLRKYQEVMQE"
     gene            43611..44300
                     /locus_tag="ACZPCG_01150"
     CDS             43611..44300
                     /locus_tag="ACZPCG_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1283713.1"
                     /translation="MSKILIVEDEESIAELEKDYLELSGFEVEIENNGTDGLDRALKE
                     DYDLLILDLMLPGTDGFEICRRVREIKNTPIIMVSAKKEDIDKIRGLGLGADDYITKP
                     FSPSEMVARVKAHMARYERLIGSGQPANEIIEIRGLKIDKTARRVWVAGEEKTFTTKE
                     FDLLTFLAQNPNHVFTKEELFSKIWDMESIGDIATVTVHIKKIREKIEFNTAKPQYIE
                     TIWGVGYRFKV"
     gene            44658..47291
                     /gene="ppdK"
                     /locus_tag="ACZPCG_01155"
     CDS             44658..47291
                     /gene="ppdK"
                     /locus_tag="ACZPCG_01155"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HIR1283714.1"
                     /translation="MATKWVYLFSEGNATMRNLLGGKGANLAEMTNLGLPVPQGFTIT
                     TEACTQYYEDGRKINDEIMGQIMEHIGKMEEITGKKFGDKENPLLVSVRSGARASMPG
                     MMDTILNLGLNEEVVEVLAAKSGNPRWAWDCYRRFIQMFSDVVMEVGKKYFEELIDKM
                     KAEKGVTQDVELTAEDLHELANQFKAEYKEKIGKDFPTNPKEQLMEAIKAVFRSWDNP
                     RANVYRRDNDIPYSWGTAVNVQMMAFGNMGETSGTGVAFTRDPATGEKHLMGEFLMNA
                     QGEDVVAGVRTPQKIDQLKEVMPEVYEQFVGICHTLEDHYRDMQDMEFTIEDKKLYML
                     QTRNGKRTAKAALKIACDLVDEGMIDEKKAVQMIDPRNLDTLLHPQFDAEALKKAEPV
                     AKALAASPGAACGKIVFTAEDAKEWKERGEKVVLVRLETSPEDIEGMKASQGILTVRG
                     GMTSHAAVVARGMGTCCVSGCSEIIMDEANKKFVLAGKEYHEGDWLSIDGSTGKIYDG
                     IIPTVDATIAGEFGRIMAWADKYRRLRVRTNADTPRDAAKARELGAEGIGLCRTEHMF
                     FEGNRIDAFREMICSETVEEREAALEKILPEQQGDFEKLYEALEGNPVTIRFLDPPLH
                     EFVPTEEEDIKKLAEAQGKSVETIKAICDSLHEFNPMMGHRGCRLAVTYPEIAKMQTK
                     AVIRAAINVKKAHADWNVCPEIMIPLICDIKELKFVKKVVVETADAEIAASDIDLKYE
                     VGTMIEIPRAALTADEIAKEAEFFCFGTNDLTQMTYGFSRDDAGKFLNAYYDTKIFEN
                     DPFARLDQTGVGKLMEMAIKLGKSTRPDMHVGICGEHGGDPTSVEFCHKIGLDYVSCS
                     PFRVPIARLAAAQAAIANAGK"
     gene            complement(47564..48508)
                     /gene="istA"
                     /locus_tag="ACZPCG_01160"
                     /pseudo
     CDS             complement(47564..48508)
                     /gene="istA"
                     /locus_tag="ACZPCG_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     assembly_gap    48509..48518
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            48655..48882
                     /locus_tag="ACZPCG_01165"
     CDS             48655..48882
                     /locus_tag="ACZPCG_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HIR1283715.1"
                     /translation="MVISKHRTSDGFYPGLFNLISIFDGKRRKAVIQSILEVKKYLDD
                     MEMLEVVSRVLEKLDSMSDEEFEAMEFVAAE"
     gene            48969..49208
                     /locus_tag="ACZPCG_01170"
     CDS             48969..49208
                     /locus_tag="ACZPCG_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1283716.1"
                     /translation="MNDKGVGAIFCLISSILISAKYLSAAIFVSNTSNKDAILFSASL
                     EYVGSFLTIASLIALIIGLLFLGLKISRSFFYGYT"
BASE COUNT        13899 a        10105 c        13093 g        12170 t
ORIGIN      
        1 ttaaaagaga aggagcctgt tcgattgacc ctgcgtaccc ttggagaact gggtaaagtt
       61 actgcacaga agcgggaagt atatgtggta gaaaatccgg ctgtattttc cattttgaca
      121 agccggaatc catcttgtgc ggcggtctgc atcaatggac agccaagact ggcatccttc
      181 ctgctgttag attttctgag ggaaacgcat caattcttgt atgcaggaga ttttgatccg
      241 gagggacttc tgattgccca gagattaaaa gaacggtatg aagattcgct gtatttatgg
      301 aaatatgagg cagagtggta taaaaagtat ctgtccggtg taaggttaag cggcagccgg
      361 atcaaaaagc tggatcatgt atacagcccc gaattgctgg aaataaaaaa atggatgcag
      421 aaagagcaga aagccgctta tcaggaggcg atgatcgaga tttatctgga ttaggaataa
      481 acggtaaggc cgaaaatgcg gatatacatt ttcggcctta tgtttttcct atggaagctg
      541 cataatattg attaaaataa tccaggacaa tccatagtag gaaatgacgg ccaccagatc
      601 gtttaccgtt gtgataagtg ggccggatgc tactgccgga tccactccta ttttctggaa
      661 gaaaatagga atgattgtac cggataaaga agagataagc atggcaagta ccatagctgt
      721 gccaaggcat ccggaaaccg caaaggcaaa ggggacggta tttcccttta gaaccagata
      781 acctccgatt atgagaaatg acagggcacc aaggattaaa ccgttggtca atcctacccg
      841 tgattccttt atgatcagtg ccagtttctg ctttcctgaa agctgcttat ccataagtac
      901 acggatcgca acagcaagag actgggttcc tacattacct gccatgtcca gaatcaagga
      961 ctgaaaacac ataataacag gaagctgtgc gacgatggat tcaaagaggc ctacggttgc
     1021 cgatacgcca agacccattc caagcaggat aaaaagccag ggaaggcgtt tttttacgct
     1081 gagaaaaatg ggttcctcca gatcctcttc agcagaaaga ccggcaaact ttgcgtaatc
     1141 ctcatttaat tcgtctccaa cgacttctac cagatcgctg gcagtgataa caccgattag
     1201 tttgttgtca ttatccagta ccgggataga atcttcagag taatctctaa gctgagggat
     1261 acagtcttcg atggatgcac gcgcataaat atatggatag gaacaggtca tgataccttt
     1321 cagatccgtt ccttctctgg ctatgatcag atcctttaaa tcaatagctc cataaaatat
     1381 attatattga tcgacaacat agatggtgct gatattgtca ttttccgctg cctgacgaac
     1441 cagctcagac atggcttctt tgacagaggc gtgatcggaa acaacgatgt aattagtaga
     1501 cataacgctt ccgatttcat cctcatcaaa ggaactcagt aattttacgt cggagcgtac
     1561 ctcaggatcc agtaagtcga tgagcgtttc ccgttcccct ttttccaggt gccggagtac
     1621 atccactaca tcagaggttt ccatacggga aaatacatcg actttttttc ggatgcttaa
     1681 ttcctggacg tattctgcaa tatggtcgga atattctaat atatccgcca gtgcttctga
     1741 atccaggatc tgatataatt ttgtgcgttc tgttttattt aaaagatcca gagccaatgc
     1801 gatatcattt tcatggtagg aagataattg ttcctgcatt ctctttggag tcagattgct
     1861 gcggatcagc tctgcgattt ccaggctgta tcctgctgct gttgtgtttg ctgccatttc
     1921 attgtcctcc tttacaagct gatttgggca aaggcgggta cagaaattct gttcgcagca
     1981 aaaagggcat aaaaaaacca ctgccggctg caaacaaaac agactgcaat cgcttttgct
     2041 tatgtagtgc tgtgttggaa cagacaatgg tattcactca tatatacaga cgaaatcagg
     2101 ccatggacag tcggaagctg tctgtgtatg taaatggttg gaatacctta tccgtacttc
     2161 gactatcact accgtccatg atctcacctc acttttaatt gttttattat atgactaaaa
     2221 tgacctgtat attataacct atttttccgg gaagcacaac cttttttctg ttaaatcaga
     2281 aacgctgcat ggaatcgtga tcatggtgat gtaatctgcc tgatttttaa gtgccatttc
     2341 atttagcccc tcttcatagg catagccggt aagaagcaga tgatgctctt tggcatagga
     2401 aaggattgac cggtatacct gaggaagctt atcccatgtc ccaatacaga aggagcgcag
     2461 ataggttccc gccggcagta tttcgtcgta tgcctcggag gtatgagggc agtaagcaaa
     2521 aaaggaactg tagctgttaa aatgctttct tcggatagaa tccatagaaa tacggcttcc
     2581 gaagctgtca taaagatgaa atgtctcttt cagcctgaga gaaaaatcag cagcaactga
     2641 aaaatccgcc tcatcatatt ttcccgtaat ggcagaagaa ataagaattt tttgctgagg
     2701 aagtgagcac agctgaatgg agccgtgttt tgcgttcata gcaattttaa gacgttccgc
     2761 tttttgttct aaaaatgact gttcagataa aagctgctga atggattctt caatccataa
     2821 tttccttttc tccattatcc gggaaaagga atcgtccgat ggactggaag catattcccg
     2881 gatttcttca atggacaggc cgatttttcg cagtgtcagg atcatttcca gctgtgcgaa
     2941 ctgaaagcag gtgtaatagt gatagccatt ttcttcaatg acctcaggac agaataagcc
     3001 gatatcgttg tagtaatgaa gagttcgttt gttgactccg gcacgtttgg caaattctcc
     3061 ggaggagtat agatagtgtt tttgctccat aaaaactcct tgactgtaca cttactgtac
     3121 attatatgat agggcatata agaataatgt caataggaag aaggagattg tatggaattt
     3181 acatcagtta aaaagcagca gtggtcggaa ataaaagaaa tttatatgga agcatttcca
     3241 aaatgcgaaa gaaagccctt ttttttactg cgccagtcaa ttaagagaaa aaaagccgtg
     3301 ctggcggtgg cagcagaggg aaatcagctt ctgggattta cagtttttgt tgtatataaa
     3361 gatatggtta tgatagatta tctggcagtg tcatcgaaaa tacggagcag aggaacggga
     3421 agccggattc tggaacatat atgcgggcag tttccggata aaaaaatcgt gctgttaatt
     3481 gaacgtgtgg acgatatggc agagaattgt cagcagcgga ctgcccgccg gaggttttat
     3541 ctgaaaaatg gtttcacttc ttccggcatt tttataacgg gtgcaggcgg agacatggaa
     3601 gttttaaatt acggaggaac tgtttcgcag gaggaatata tggatttaca aagatatgcg
     3661 cttgggaagc tgtttttccg tttgtcgcgg attcagctgg cagaggtgtg agtatttatt
     3721 tatgatgcag gaagggtgct tttaacaaat aaaatatatc aaaaatctgc aattcagtgt
     3781 tataataggg gtactgaact catttgacag gccgtacggt aaaataagcg gcaaagggct
     3841 gcagaccggt gatgtcatgt cggcctgatt ccccggcagg gggatgacgt tggaccatta
     3901 taagaacaga aaggccatat aaaactcgaa aatggaacca tattactaca caaaactacc
     3961 caaacaccat cagtccgtct accatgccat tttccagggg cttatgaatc tggaggattc
     4021 ctttttagtc cccagaattg acggcaggga gctgacagac ctgtttttcc gtctgcgtct
     4081 ggatcatccg gagatattct gggccagtgg atttcattat aaatattatc aggattcgcc
     4141 caatctgatt tttgaaccgg agtatctgtt tgaaaaggga aagataaagg agcataaaaa
     4201 ggcactggcg tcccgggtgg agaagctgat ccggccggcg gcaaagcttt ccgagtggga
     4261 gaaggagaag tatattcacg attttatctg tgaaaatgtc cgctacgata agctgaaaaa
     4321 ggcctattcc catgagatca tcggtccttt gggccagggt gtgggagtat gtgaggggat
     4381 tgcaaaggca gtgaaggtgc tctgcgatgg ccttgggatc tggtgcatga ttgcaatctg
     4441 cggaaataat ccggaaaaag gcatcaagta ccgtcatacc tggaatattg tgcagatcgg
     4501 aggcgtgtat tatcatctgg acactacctt tgacaatacg ctgggaaagc ataactgcgc
     4561 agaaggcggg atccggtatg actattttaa tctggatgac aaggcagtat ttcgagatca
     4621 tgagccgtta atcgctccgg cgccgtcctg tacagacggg gatcattttt attataaaga
     4681 aaagaagctg tcatttacaa aggcagagga tgtctataag agagcctgtc aggcagcgaa
     4741 aaagggaaag ccccttactt ttcactggag aggcggttat cttaccaggg ctgtgctgga
     4801 ggaactttta gatttaatga aaaaagcagg agaagaacgg gaaaagacag cgcggattgc
     4861 gcttaactgg cctcaggcgg tgattcgcct gacctatgcg gacggcaggg cggagagcag
     4921 tgtttccgta gacgaggcta atgagggaga gcaggaaggt acataacata aatgacacaa
     4981 caggaaattt taaagcatta ttttggctac gacagcttca gggaagggca ggagcgttta
     5041 attgaagcaa tcttagaagg cagggacgta ttgggaatca tgcctacggg ggccggaaag
     5101 tctctttgct accagattcc tgcccttatg atggacggga ttaccctggt gatctcgcct
     5161 cttatttctc tgatgaagga tcaggtgagc aacttaaatc aggcgggaat cctggcagct
     5221 tacttaaaca gttccttaac ctacggccag tacagaaaag cgctggagtt tgccagagca
     5281 gggcggtatc ctattatcta tgtggctccg gagcggctga tgacagagga atttttacag
     5341 tttgcactgg atcctcaggt aaagattgcc atggtggctg tggatgaagc ccactgcgta
     5401 tcccagtggg gacaggattt caggccaagc tatttaaaaa tagtggattt tatccggcag
     5461 cttccggtgc gcccggtggt cagcgctttt acagctacgg ctacggcgga ggtacgggat
     5521 gacatcatgg atattctgat gcttcgccag ccggagatcc ttaccacagg ctttgaccgc
     5581 gggaacctgt attttggcgt gctgtcccct aaggacaagt atgcggctct ggtgaattat
     5641 ctggagcgcc ataaggggga aagcggtatt atttactgta ttacccgcaa ggtagtggag
     5701 gaagtctgcg ccaacctgat ccgggagggc tttcctgtga cacggtatca cgcagggctt
     5761 ggcgatgaag agcgcaggca caatcaggag gagtttattt acgatcattc ccctattatg
     5821 gtggcgacga atgcctttgg aatgggcatt gataagtcca atgtgcgctt tgtggttcac
     5881 tataatatgc cgaaaaatat ggaatcctat tatcaggagg cgggacgcgc gggccgtgac
     5941 ggagaaccgg cggagtgtat ccttctctat ggaggtcagg atgtgattac caatcagttt
     6001 tttattgacc ataatcagga aaatgatgcc ttagatccca tgacgcggca gatcgtgatg
     6061 gagagagacc gggagcgcct gcggaaaatg acattttact gctttacaaa tgattgtctc
     6121 agggattata tcctgcggta ttttggagaa tacggcagca attactgtgg aaactgttcc
     6181 aactgcctga ctcagtttga aactgtagat gtgacggaga ttgcagagac gctcatccgc
     6241 tgtgtgcaga ccagcggaca gcgctatggg accaatgtga ttatagatac ggttcacgga
     6301 gccaacacgg caaagatccg caattaccgc atggatggaa atatctgcta cggaaagctg
     6361 gcaaaagttc ccgtatacag gctgcgtcaa gtgataaacc atctgcagct gtccggtttt
     6421 ctcaccgtta ccaatgatga atatgccatt gtgcatctta cagaaagctc tggaaggatt
     6481 cttgcaggag aagagacggt tatgatgaag atggcaaagg agcaggagca cacaaagacc
     6541 gatacgggaa gcagaaagaa gaaggccaaa ggtgttttgg caggtatggc agaggcagat
     6601 atgcccttat ttgagcggct cagggagctc cgcgcccaga cggcaaggga ggaaaaagta
     6661 ccgccctata ttgtattttc cgataagact ctggctcata tgtgcgtttt aaagcctgag
     6721 gacagggagg aaatgctgtc tgtttccggc gtgggcgaat ttaagtatga aaagtatgga
     6781 gagcgttttc tggcctgcat cagagaatgg aaggagaaag aagcataaaa tggatcagga
     6841 taagagaaaa gagatcataa acagttcaga gacacaggag gatttgggtg tacgggagga
     6901 tggcgtacag gtgacaggtg tacaggagtc cggtatacag agtgaatccg ccgttcctca
     6961 caaacgccgt gtccgctaca agggtaccca cccgaggaaa tttgaggaaa agtataagga
     7021 acatcagccg gaaaagtacc gggataccat tgaaaaggtc atcagtaagg gcagcactcc
     7081 tgcaggtatg cacatttcca ttatggtaaa tgaaatcctg gatttccttc agatccagcc
     7141 gggacagacc gggctggacg ctactttggg ctacggaggc catacgggcc atatgctgga
     7201 gcagttaaag ggacagggcc atatgtgggc gctggatgta gataccatcg agatggaaaa
     7261 aacaagaaag cggctggcag acaggggatt cggacctgac attttgacca taaagcatct
     7321 gaattttgca aatattgacc aggtggcaga ggaagccgga ggctttgatt ttatactggc
     7381 agatctggga gtttcttcca tgcagatcga caacccggag cggggatttt cctataaaac
     7441 cgatggccct ctggatctgc gcctggattc ttccagaggg atgacggcag cggaacggtt
     7501 aaaggagctg gacgaggagg agcttgtggg gatgcttgtg gaaaactcgg atgaacctta
     7561 tgcgaaagaa attgccagaa gggtgatgcg cgagctgaga aatggaaatg ccgttgacac
     7621 aactacccgc cttcgggaac tgattgagga agccctggta tttcttcctg tggctgatcg
     7681 gaaggaggct gtgaaaaaat cctgccagag atgctttcag gcactgcgta ttgacattaa
     7741 cagcgaattt gaggtgctgt attccttttt ggataaactg ccgggagcct taaagcccgg
     7801 aggaagggct gcgattttaa ccttccattc cggagaagac agactggtaa aaagggcatt
     7861 taaagacgga aagaaggcag ggctttacag tgagatttcc gctgatgtga tccgtccctc
     7921 ggcagaggag tgcataaaga acggacgtgc ccgttctacc aagcttcgat gggcgatccg
     7981 gggataaggt tttatacgga ccaattgact gcaggaggtt tttgcagaag gagaagggag
     8041 gcatgcaaaa tggacatgga acagctgtcg ctgtttgata cggaagcagt ttacgatccg
     8101 ctggcaagcc ggatgcgtcc ggaggatctg gatgagtttg caggccagac gaatcttttg
     8161 ggaaagggga agcttttgcg ccagctcatt gagcaggacc gcatcccgtc tatgattttc
     8221 tgggggcctc cgggggtggg aaaaaccacg ctggcaggga ttattgcaaa gagaacccat
     8281 gcagaatttg tgaatttcag cgctgtcacc agtggaatca aggaaatccg cgaggtgatg
     8341 gcccaggcag agggcagacg gcgcatgggc ggaaaaaccg tgcttttcgt agacgagatc
     8401 caccgcttca acaaggcaca gcaggatgcc tttcttcctt ttgtggagaa gggaagtatt
     8461 atcctgatcg gggcaaccac ggaaaatcct tcctttgaga tcaacagcgc tcttttgtca
     8521 cggtgccgtg tatttgtcct tcacagcctt tcaaatgaag aactggtgca gctgatccga
     8581 agggcgctca catccccaaa ggggcttggg tacctgaagg tggatataag cagtgagatg
     8641 attgagaaaa ttgctgcctt tgcaaacgga gatgccagaa cggccttaaa tgttctggag
     8701 atggcagtgg caaatgggga aattaccggg gaaaagacca ttgtgaccat ggatactctg
     8761 atgcagtgta tcgggaaaaa gagcctgctt tatgataaaa agggagaaga gcattataat
     8821 ctgatctcgg ctcttcacaa atccatgcgc aattctgacc cggatgctgc ggtatactgg
     8881 ctggctcgga tgctggaggc aggagaggat cccctttacg tggcaagaag gctggttcgg
     8941 tttgccagtg aggatatcgg gatggcagac agcagcgccc ttcctctggc agtggctgct
     9001 tatcaggcct gccattttct gggaatgccg gagtgcaatg tccatttaag tcatgcggtc
     9061 atctacctgt ccaccgcgcc caagagcaat tcttcctata tggcctatga ggcagccaaa
     9121 gaggatgcaa aggagatgct ggcagagccg gttcctcttg tgatccgcaa tgcgcctaca
     9181 gacctgatga aggagctgca ttacggagac ggctatgtgt atgcccatga tacaaaggaa
     9241 aaaatcgtgc ggatgcagtg cctgccagac agcgtaaaga accgtgtcta ttacaggccg
     9301 ggaattcagg gggatgaaaa ggtcataaag gacagaatgg aacggatcaa acagtggaga
     9361 aataaaggag tgtcggagca ttaaagctcc ggacactcct ttaaattttt caggatacgt
     9421 tccagatttt ttttaagaag ctcttgttct tccggatcca ttccctcaca gattacatca
     9481 ttaagacggt caaagccctg atggacctgg gccgcctctt cacgtcctgc cttggtgagg
     9541 cagatgagaa aggatcttcg gcagtccgga tcacgttccc gtaccaaata tccggatttt
     9601 tccagcttat ccagactccg ggacatggtg gttgtatcca tacggcagcg ccccgccagc
     9661 tctgtctggg tcagatggtc ttcctccaga aggcagttta aaatcctggc atgtccctgg
     9721 cccagagtaa gtcccagatt ctgtaaaaat ggctgtacca gccgttttcg ggccttttca
     9781 atctgtatca tcagatctct tatatctgta tttccattca taatagaggg tcctttctgt
     9841 caatccaatt caaattgtcc ggtataaagc tgatagtacc gtcccttctg ttccagcagc
     9901 tcgtcgtgat ctccccgttc catgatttcc ccgtgctcta atactatgat cgcctcgctg
     9961 cggcgcaccg ttgagagacg atgggcgatt acaaatacag ttctgccctc catcagggcg
    10021 tccataccct gctcgatgag cttttctgta cgggtgtcaa tggagctggt agcttcatcc
    10081 agaatcagaa ccggaggccg tgagatggcg gccctggcaa tggctaagag ctgtctctgc
    10141 ccctgagaca gattggcacc gtcgctgtgg agcaccgtgt catagccctg gggcagacgg
    10201 cggataaagg agtgggcgtt cgccacctgg gcagcggccc gtacatcctc gtcgctggca
    10261 tccagacgtc cgtagcggat gttgtcggcg atggtgcctg taaataaatg ggtatcctga
    10321 atgaccatgg acatggaacg gcgcagatca tcctttcgga tttcccttat atcaatgccg
    10381 tcataggtga tagaaccgcc atttacttca taaaaacggt ttaccaggtt aataatggta
    10441 gtttttcctg ctcctgtaga tcccacaaag gcgattttct gtccgggctt ggcatatagg
    10501 ctgatgccgt ttaagatggt cttttcaggc acatatccaa agacaacatc attgaaacgg
    10561 acatctcctt tcaggggaat aaaggcaaag ccatcggaaa caggagcatc cagtctggtt
    10621 cgttttaccg tttcctcagg aaccttccag gcaaagcggc cggtaaattc gctgcattcc
    10681 ttaagctgtc ccatcggatt ttcccgaacc tggcataagc gtacgtttcc ctcgtcgatt
    10741 tccggtattt catccatcat gtcaaaaatt cgttctgcac ccgaaagggc agacaaaaga
    10801 aagttaatct gctgggtaaa ctggttcatg ggcatggcgc tctggcgcac atagaccaga
    10861 taggaggcca gtgttcccag atccgtcagg ccggccagtg caaagaggcc tcccacacag
    10921 gcagaaacgg cgtagttaaa ataggaaagg gaaacaatgg tggggatcgt catgccggaa
    10981 taggctaaag cttttgttcc ggcatcccgg agggcttcat ttctgcggca gaattcttca
    11041 aaatcctgcg gttcatggtt aaagaccttt tccaccttct gtcctgccac catttcctct
    11101 acaaagccgt taatgcttcc aagacactgc tgctgccgga taaaatattt ccggttgcgg
    11161 cggctgttgt atcggatgaa tagggtcatg caggcaagga agaaaatgac gatcaaagaa
    11221 agacgccagc ttaaaataag gagcatggta atggtaccgg taatggtaat aaagctctgg
    11281 atcatcatgg caaagctgcc gtttaaggcc tctgtcagag tgtccacgtc attggtaaag
    11341 cggctcatga gctcgccgtg ggtatgggcg tcaaagtagg ataggggaag cttttgggta
    11401 tgctcaaaca gatcagaacg gatttcgctt actacctgct gggacatatg gagcatgagc
    11461 tggttatagg ccagacaggc tgaggcgccg agtgcataca taagccccat gagggcgatc
    11521 atgcggatga gtcccggaat atctccgggg agaatgtagt tgttgatcac aggctttaga
    11581 aggtaggtac ccatgatatt tgccatggcg cttgcagcta ctaaaagggc aatggcaaac
    11641 atataccatt tatgccgtcc tagataccca aacatctgct tgattgcccg tctggtgttt
    11701 tttggacggc tgaaatttgc tattcttgcc attacagatt cgctccttcc tgctgtgaat
    11761 aatagatttc ctgataaatg gagttgtgtt tcaaaagctc ctcatgggtt ccgcacccgg
    11821 ccagccggcc gtcctccagg ataaaaatct ggtctgcgtg gagtaccgag gagatgcgct
    11881 gggcaatgat gatcttggtg gtgtccggaa ggctttttgc cagcccttcc cggatctttg
    11941 cctcagtggc agtatccaca gcgctggtgg aatcgtccag gatcagaact ttgggggctt
    12001 ttaaaatagc tctggcaatg cacaggcgct gtttctgtcc gccggacaca ttgacgcctc
    12061 cctggcccag ctcggtttcg tagccctttg ccagccgtcc gataaattca tccgcacagg
    12121 cgatccggca ggcggcatca atttcttcat ctgtggctgt ttcctttccc catcgcaggt
    12181 tatcctttac ggtgccggaa aaaagcgtgt ttttctgcag caccacagca atggagtcac
    12241 gcagctctct gagaggatat tcccatacgg gatggccgtc taccagcacc gttccttttg
    12301 tagcctcata aagccttggg atcagctgta ccagtgtgct tttggcagct ccggtctgtc
    12361 cgatgatgcc tacggtctgt cccgccttaa tatgcaggga aatgtcagaa agcacatcct
    12421 cagagctttc cttattgtat ttaaatgaca catggttaaa ttcaatttct ccccgttcta
    12481 cccggatgtc tgttgcccgg tcttctttga tatcaatgtc ttcatgcatc acttccatga
    12541 tccggtgcca ggacgccatg gctctcgtaa tcattaaaaa cacattggaa aacatcatca
    12601 gggaatttaa aacctgaagc acgtagctta aaaacccggt cagttcgcca acgccgatgc
    12661 ctccgatggt aaggatattt ccaccgaacc acaggatgga aagaatggtg ccgtacatta
    12721 caaactgcat ggcaggcata tttaaggctg ccagccggaa ggcctgttca gaggcggtcc
    12781 ggagctcggt gtttacctgc tcgaatttgg cggtttcgta atctccgcgt acataggatt
    12841 ttactacccg gatggcagtc agattttcct ggatgatccg gttgacctga tccatggcac
    12901 cctgcatccg ggagtagagc ggccgtacat ggctgatgat gaaaaacagc ataatgccca
    12961 aaataggggc agccactaaa aagaccatag ccagcctggg attgatcaga aaggaagcgg
    13021 tcatggctgt gagcatcatg ataggggagc gcagtcccgg cctcagtcct gtagcgaccg
    13081 agttctggat ggcagtcaca tcgcccgtaa ggcgggtcac cagagaagag gtggtaaaat
    13141 ggtcggtgtt tgcaaaggaa aattcctgca gacgggcaaa ctccgcccgg cgcagttcgg
    13201 ctcccagccc atggccgaac cgggcggaaa aaatggcaga accctggccc aggatcaggg
    13261 cgatcagagc aaaaaggatc atctgtgcgc cttttgccag aatataacgc cggtctccgt
    13321 tcgccacgcc tacatccacg atcgaagcca ttacaagagg gatcaccagc tcaaaaatgg
    13381 tttctgtgac aatgcagaac gcagccagaa tcccatcccg gcggtaaggg cgcagataag
    13441 acataaattc tttcataaat cgtaagcctc ctgtttatgg gtaatagcaa tagttgcata
    13501 tacaactatt gtaaagctga ggatgggaaa ggtcaagatt atttgggaaa gtaaggaaat
    13561 atatgaaagc ataaatgaaa tctaatgtga aaaagaccgg ggatatggta tacttttata
    13621 aaagacaggc gtacaaaggg cgatagtgac agcataaatt acagaagtgg agagttccaa
    13681 tatgaaagac aagatcattc atctgattgc atcagaagac agcagaaatc ccatgacaga
    13741 tgaagaaata gcggagactt tgggaatttt tagagaaaca gttacaaata taagaaaaga
    13801 ggcaggactg ggagattcaa gggaacgaag aaaagcggca gtgtttcagg atatgaagga
    13861 gcttctcaat atggatccgg atcttacaga gcggggatta acacgatgtt tggctgaacg
    13921 ggggtatgta attggaaaat acgcagtggg aaaattaaag gaagagcttt taaaggagtg
    13981 gcagcctgaa ccggaatatg ggaaaacagg agaagaaatt caggatggga gccgcttttt
    14041 ttcaaaggtg atcggttatg cgggaagtat gaaaagccag atcaagcagg tacaggcagc
    14101 aatcttgtat ccgcctaaag gtcttcattg tcttctttac ggtccttccg gcgtaggaaa
    14161 gagctatctg gcagaaatca tgcatgaata tgcagtgaca acggacaatt ttgcttcgga
    14221 tgccccatat tttgcattta actgtgcaga ttatgcagac aatccccagc ttcttttgtc
    14281 acagctgttc ggttattcca aaggtgcctt tacaggtgca tctgagaata aaaaaggaat
    14341 cgtggaacag tgtgacgggg gaatcctctt tttggatgag gttcaccggc ttccaccaga
    14401 aggacaggaa attttgtttt atttgatgga taaggggaaa ttccgccgcc tgggagagac
    14461 agaggcacag cgggaaagtc atctgatgat tattgcagca acaacggaag atccaagaag
    14521 ctccctgctt cttacattca ggagacgtat tcccatgatg attgaaattc catccctgaa
    14581 agaacggcca ttaaaggaaa agctgcagtt tatccgtctg tttttctgga tagaatcgag
    14641 acgtttgaat gcgcctgtgc tggttcgggc agaagtgatc cgctatatgg cagatgagga
    14701 ataccaggga aatgtggggc agttaaaggc agatatacaa gtctgctgtg caagagcctt
    14761 tctggacggt agaacaaaaa gaagatcaaa gatgattgtg ggaatggagg ctctttctgg
    14821 aaacaaaacg gagcctatat cttctggaga acattcccgt ctggcggagc tggtaccgga
    14881 agatgagata tttacaccgg atatgccggc agttacgaca gctgaaacgg ataatggctg
    14941 ggatatttac cgtgatctgg aggaacgcta cagtgctttg acaaaagagg gattaaaaga
    15001 aaatgaaatt gaagaaaagc ttttgagaga aattgagagc agttttcagc tccatatccg
    15061 ccaggtggag gaagcgagct taagcctgga agaactttct cacattacag gaagcgacgt
    15121 aatggagatg gcaaaagaag tgtatgaact ggccagtcaa aggcttccgt atctgaagcg
    15181 ggagatgatt attccgctgg caatccatct gaaaatggcc tgtgaacggt taaaacagca
    15241 ggagcggatc gtagagaagg gaattaagaa tctgggagaa ctttacaggg atgaatatga
    15301 ggctgcaaaa gaaatcctga attatatctg caaaaaatac tatgtttgtt tttacaaaga
    15361 agaagccgga tttttagcaa tgtattttca ccgctttcag gaaaagcaca ggaaggaagg
    15421 ctgtgtgggt gttgcggtag tttcccatgg acctgtagcc agcgccatgg cagaggtagc
    15481 caactctatt atgggagtca gtcatgcggt aggagtggat atggggatca aagatacgcc
    15541 ggttcagatg gcggaacgaa caatggaagc cgtgcgccgg atcgaccagg gaaaaggcgt
    15601 tttgattctg gcggatatgg gttccctttt agatatggaa gaaaaggtga aaaaggaaac
    15661 aggaattact gtaaaaattg taggaagaac ggatacaatg atggttattg aagcaatccg
    15721 aagaacctac tggacagatg aaagcctgga aacaattgca aaggtacttg actggaagca
    15781 gttctcacct gcaaagcgtc agacgcgtaa acccagagaa aaagcagttc tctgtttgtg
    15841 tattacgggc gagggagctg ccagaatgtt aaagagctat ctggaagagc gtttaaaaag
    15901 cactctgggg aacgtgagga tcattacaaa aggatatatt gaaaatacgg atattacaga
    15961 gctgatccgc gcatccgaag aagaatatga aattttagcc attgcgggaa ccattgaccc
    16021 aaagcagacg cagtacccgt ttttatcggt ttcagaaatt tatgcaaaag agggaatcag
    16081 gacactgcgt cagattttga aacgccattt cttcaaagag cagaattgcc ttcaggatgt
    16141 gatttttaga gatctcattt ttatacagga agattttgct tacaaggagc agattttaga
    16201 tcagtgtgtg gaagcaatgg ctgaaaaggg attggttcag tcggagtttc ttttaagcgt
    16261 ttataaaaga gagggcatga tgcctacatt tttgaaggga gggatcgcaa ttccccacgg
    16321 tgcggaaaat ctggttacaa aaccagtcat atgcattaca aaattggaca aacccattgt
    16381 ctgggatgga tttaatacgg tagatcttgt atgtgttctg gctttaaatg aaaaatcgac
    16441 aaaatatttt gagcagtttt atcaggttat ttctgatgaa atcatggttt ccagaatacg
    16501 ctcatgccat accccagatg agatatatgg aattttgtgt aattatacaa aaacagtcaa
    16561 ataagttggc atcattcttg ctcatatata aggtataacg agcaggaaag gagctgatta
    16621 aatgaaagta aatgattttc ccaaagtaac tgttatctta agaggctata cctacgatca
    16681 aatacatacc gttgtaaaaa atctgatagg gacagagctt aaggcggttg aaattaccat
    16741 gaacacgcct ggagctttgg aaacgattcg gaaaatccgt gaggaatttg gagattccat
    16801 tctggtagga gctggaacgg ttcttacata cacggaagcg cagcaggcaa ttgatgcagg
    16861 tgcagccttt ttactttctc caactgtatt tgataaggaa atactggatt tgtgcagaga
    16921 aagaggtgtt atatcagttc cgggagcatt ctcaccatcg gaaatatgga aaagcattca
    16981 ggatggagca gatatcgtaa aggtattccc ggcaggacga ctgggaagca ggttcttttc
    17041 agatatccag gcgcctcttg gcagactgcc tctgatggca gtaggaggag taaatgtgga
    17101 aaatctggaa gaatttttta atgcaggagc atcatttgcg ggaatcgctt ccggtatttt
    17161 taaccgtcag gatatcattg agcagagaga agaacactta agaacatcaa tcagggaagc
    17221 agaaaagaaa atcaggcagt taagggaagc acaggaggaa tgaaatgagt gcaattatat
    17281 ttgatgaatc gtttatttta agattcgaca cccatttaac gaatacagag gtattatcgg
    17341 ctatggccga taatctctgc aaaaggggaa ttgtaaaaga aacatataaa aaggcaattt
    17401 tagaccggga acaggagttt ccaacagggc ttaatacagg gggaattaac gtggcaattc
    17461 ctcatgcaga tatcagccat gtaaataaag cggcaatctg cgtcggtatt ttaaaggagc
    17521 ccgcagcctt tcatgcaatg gatgagccgg attatgatgt tccggtaaag ctggttatta
    17581 tgctggctct tacagaagct cacggacata ttgaaatgct tcagaagatc gttggcctga
    17641 ttcaggatca ggcagcagta aaagagatta cagaaacaga tgattcagaa aaagcaggag
    17701 agttgatcag cagattttta ttagaggaaa aggagaatta agagatgaag agaatattag
    17761 tggcatgtgg aaatgggatc gcaacatcaa ccgtagtagc aacaaagatc cgtgagaaat
    17821 gtgaggaaaa cggcattgct gttacagtca cacagtgtaa gcttttagag gtaaatgcca
    17881 aggcagataa ttttgacctt ttagtaacaa caggaaagtt cacaggagga aatgtaaata
    17941 ttcccgttgt aggcgctatt tctttattaa cgggaatcgg agaagaggaa actctggatc
    18001 aaattcttga ctatgttcga taaaaaacat actggggagt attacagata gaaaaagagg
    18061 aatgcagtta tgcagattat ttacaatata tttaatgtga ttctggatgc aggggcggtt
    18121 gtaatgctgc cgattatcat cacaatcgta ggactgattt ttcaggtaaa attatcaaaa
    18181 gcgttccgtt ccggtttgac aatcggaatt ggttttgcag gcatcaatct ggttatcaat
    18241 atgctaaaaa gcaatctggg tcctgctgct caggcaatgg tagaaaattt cggcattaag
    18301 ctggatatcc tggatgtggg atggggagcg atcgcggcag ttacatggtc ttctccgatt
    18361 attgcaattt taattttctg tattctggct accaatatca ttatgctggc attaaaggct
    18421 acagatacgt tggatgtaga tatctggaac taccaccaca tggcgattgt gggaatcatg
    18481 gtttattttg tgacaaaaaa tgtggctttg ggaattgctg ctaccgttgt aatggctatc
    18541 gtcacattta aaatgtccga ctggactgca cctttagtag aagaatattt cgggattccc
    18601 ggagtgtccc ttccaactat gtctgcattg tcatccatcg taattgcagc accgttaaac
    18661 tggattttag accggattcc cggaatcaat aaaattgatt ttgagattaa ggacgcccag
    18721 aaataccttg gctttttcgg tgagccaacc atgatgggcc tgattttagg ctgtatcatc
    18781 ggtgtacttg caaaatataa tcttgcctcc atcctttctc tgggcgtaaa tatggcggct
    18841 gttatggttc tcattccaaa gatgacttct ctgtttatgg aaggacttat gccgatttca
    18901 gcggcagcca aaaactttac gcagaagaaa tttaagggaa gaaagttcct cattggtctg
    18961 gatgctgctg ttgtagtagg aaatccggat gtaattacaa cttccttaat cgtgattccg
    19021 atgactattt taatggcagc cgttcttccg ggcaaccgtg ttcttccttt tgcggatctg
    19081 gctgtggtga cattccgtgt agcccttgtt gtagctgtca caagaggaaa tcttttcaaa
    19141 aatatcatta tgggccttgt ctgtacggga gctatcctgc ttgcaggaac agcaactgca
    19201 ccggttttaa ctgagctggc tctcagcgtg ggacttgatc ttggagaagg actgattacg
    19261 tcctttgctg caaccagcct gactgtaagc tttcttgtct acaaagcatt tatcagcaat
    19321 ttgtatatca gtgttcctct tcttgtgctt gttgtgggag gtacctggat ggttatgagc
    19381 cgtatcaata aaaagaaagc agagatggca gcacagggag aacaggcctg aatatcacaa
    19441 taaaaacagg gaggaaataa gtaatgcaga gagccattga ccgattagag ccatttcagc
    19501 gggccatatc aaaagcacat ttagcagcag ccggtgtttc tgttgatgcg ctggaaaaga
    19561 aaccgctgat tgccattgca aacagctgga atgagatctg tccgggacat gaaccattaa
    19621 agaagctggc agaagaggta aaaaaaggga ttttagaggc tgggggagag cctgtagaat
    19681 ttaatacaat tgcgatgtgc gacgggattg cgcagggaca ttcagggatg cgttactgtc
    19741 tgccccacag agagattgtt acagattcca ttgaagcaat ggtagtagga gaaggagtgt
    19801 ttgacggtgt tgtttacatg gcgtcctgtg acaaaatcat accagccatg ctgaatgcag
    19861 cggcacgtat tgatcttccg tccgtgatcg ttaccgcagg cccctgtttt gctcaaataa
    19921 aacccaatga gtcaaaggaa ttaagacagc gcttcttaag gggagagatt acggaacgcc
    19981 agctcatcga gggaacgctg aaatattata cagggccggg agtctgccca ttcctgggaa
    20041 ctgccaatac aatgggggct ctgtgtgaat ctctgggaat gatgcttccc ggaagcagcc
    20101 tgattccatc cagtaccagc atgagaagat tttcagccag agaatcagga agtacggtta
    20161 tgaagctggt agaacagcag atacgtcctt cacagattat aacaaaagaa gctctggaaa
    20221 acaccgtgac gcttttaagc gcaatcggcg gatccttaaa tgccatgatt catcttccgg
    20281 ctctggcagc agaactgggg ctggagctgg attgggatga tatagcaaag atcacttcca
    20341 aaacaccggt gctttgcgga atcgtcccaa atgggaatct gacagctgta gatcttcact
    20401 atgcaggagg aataccagct gttatgaagg aactgcggga caaacttcac ggggagtgcc
    20461 tgaatgtgtg cggcctgtcc ttaggagaga ttctggagca gaccaaggac ggagacagaa
    20521 ccgtgatcca tacggccgga gaacccttgg aaaaggcaaa tggaatccag gttttatatg
    20581 gaaatctggc gcctcaggga gctttggtaa aaacatcggc tgtgcctgaa aatgtaaggg
    20641 tatttgaagg cactgcgaag gtctttgatg gagaggatgc ctgttatgct gcattcagag
    20701 atcatgctat ccatgaggga gatgcggtga tcgtgcgtta tgagggcccc aagggaggcc
    20761 ctggaatgaa ggaactgcat cgaattacgg agattataaa gggaatcccc cggacagctg
    20821 tgatcacgga cggacgtttt tccggagcga gcggaggctt aagcatcggc tatctctgtc
    20881 cggaggcaga ggaaggaggt ccaatcgctc ttgtaaagaa tggtgacagg attcaggtgg
    20941 atttatataa gggaatgatt catttggatg tttctgacga agagctgatt tcgcgccgca
    21001 atgcatggga gcatggcgaa aagaaaaaag aaaagggcct tcttggaaga tatgcagcaa
    21061 tggtgggaaa tgcaaggcag ggagccacat ttcatattta aaaactagct gtttcgagca
    21121 gagacttata aaaaatgccg cagcctgtaa gcgattccga tgggaatcct acaggctgcg
    21181 gctcttttgt atctcacctt tcccctctgc catccccgtg gccccgcagg gcctgtatgt
    21241 gtgatggagt gtaatgcaat cagggaattt catactttgt tttgtgattt ctctgccggg
    21301 taacgcagca ggggaatggt ttttcttctg tttcatccgt tcataaggtt aatgcacagg
    21361 cagcctgttt tattgcaaaa aaaatttgcc tcttattgcc agtcctatgt ttctgtgcta
    21421 atatataggt tgctttattg caggaaaaga ggaataaaaa cgtgatgcag tatttaattg
    21481 tctgccccct ggtattttta gcaggtctgg tagactccat tgcaggggga ggcggactga
    21541 tctccctgcc cggctatatg atcgcagggg tgccggccca catggctctg ggaacgaata
    21601 agatgagctc taccattggt actctggttt ccacagcccg tcttttaaag cacggatatc
    21661 tgaaaggaaa gattgctgtt gccgctgttt cggctgtgtt tgccatgaca ggctctgtgc
    21721 tgggagcgca gattgccctc cgggtaccgg attcggtgat ccggagcatg atgatcgtga
    21781 tccttcccat tgtagcctac tatgtgcttc gcaataaaaa tatgggagac agtgaacgga
    21841 cgggaaccat ttccaaaagg cgcgtctgtg tgatctcact ggcagcctcc tttgtgatcg
    21901 gaacctatga cggcttttac ggcccaggta cgggaacctt tctgatcctg gtgcttacgg
    21961 gagcagcccg gatggatatg cgcacagccg cagcccagac caaggtgatc aatctgtcgt
    22021 ccaatatctc cgcacttgtc acatttatcc ttacgggaaa tgtgtattat acattgggag
    22081 cagccgcttc cgtctgttcg gtagcaggcc attatctggg agcaggactg gtggtccatg
    22141 acggccagaa gattgtccgc cccatggtac ttgttgtgtt aggaattttg ttccttaaga
    22201 tcattgccgg agcataaaga ggaaggccgg aaaactgtaa ctgaaaacct tatttgaatc
    22261 aggagaaatg cttatgaaat ggaagaaatt tacacttaca actaccacag aggccgtaga
    22321 tctggtcagc agtatgtttg atgacatcgg gatcgaagga attgagatcg aggataatat
    22381 tccgcttaca gaaaaagaga caaagggaat gttcatcgat atccttccgg agcttccgcc
    22441 ggacgagggc attgccaaag taagcttcta tctggatgat gatgcggatg tggcggaaat
    22501 gctgaaaaag gtcaatgagg gactggacga gctggcttta tttaccgatc tgggagcacg
    22561 caccattgag gcttctgaaa cagaggacaa ggactggatc aataactgga agcagtattt
    22621 taagcccttt accgtagacc atatcctgat caaacccacc tgggagccta tccctgagga
    22681 gcataaggac aagcttctgg ttcagatcga tcccggcact gcttttggaa caggaatgca
    22741 cgaaaccacc cagctttgta tccgccagct ggaaaagcac gttcacggac aggctgagat
    22801 tctggacgtg ggaacaggca gcggcatttt aggtattaca gccttaaagc tgggagcaaa
    22861 ggaggtctgg ggaacagatc tggatgaaaa tgccattacg gcagtgggag agaatttgga
    22921 ggctaactcc atttccagcg acagcttcca tgtgcttcag ggaaatatca ttgacgatca
    22981 ggcggtaaag gactgggccg gatatgagaa atacgatatc gtggttgcaa atatccttgc
    23041 agatgtaatt attctccttg tagatgagat tccggctcat ctgaaaaaag gcggctattt
    23101 tatcacctcc ggaattatca atatgaagga agaggcagta aaggcggcct ttgcggcaaa
    23161 tcaggagctg gaagtaaccg agatcacata tcagggcgag tgggtgtctg tcacagccag
    23221 aaagaagtaa gagatgcatc atttttttgc cgatccgacc caggtggcag aggataccat
    23281 ctttattacc gggccggatg taaaccatat gaaacatgtg ctccgcatga agccggggga
    23341 agaagttttg atcagcgacg gcacaggccg cgactatacc tgtgaggtag aggcctttga
    23401 gccggaccgg gctgtggtga gaatccattc tgtctgtgac agccggatgg agcttcctgc
    23461 cgagatttgg ctctttcagg gccttccaaa agcagataag atggaactta tcatccagaa
    23521 ggcagtggag ctgggagctt ctgccgtggt tcctgttgcc acgaaaaatg cggtggtgcg
    23581 gctggatgaa aagaaggcgc aggccaagaa aaagcgctgg caggccattg cggaaagtgc
    23641 agccaagcag tcaaagcgca gccggatccc ccaggtggag ctggtgatga ccttaaaaga
    23701 ggcattttcc tttgttcatg acagggaatt tggcctgtgc ctgatcccct atgagcagga
    23761 aaaggggatg gacgctatga aacaggcttt ggagaagaca gctcccggcc agaaaattgc
    23821 agtatttatc ggtccggagg ggggatttga cgaaggggag atccgtcagg ctcttgaaat
    23881 gggcgtctgt ccggtgagcc ttggaagcag gattttgagg acagagacag cagggcttgc
    23941 cattctttcg gtgcttatga tgaagctgga aggggcattt tgagaaagga tcagcagtat
    24001 ggaagtatat tttgacaatt cagccaccac cagggtttta gatgaagtaa aggacctgac
    24061 ggtgaagctt atgacagagg attacggaaa tccctctgcc cgccacagaa agggaatgga
    24121 ggcagagcag tatgtaaaaa atgccgcttc ccagatcgca aagaccctga aggtaaagga
    24181 aaaggaaatc ctctttacct ccggcggtac ggagtccaac aatatggctc tgatcggcac
    24241 agcccttgcc aaccagaggg cgggaaaaca catcatcagt acacggatcg agcatgcttc
    24301 cgtgtatcag cccctggcat ttttagagtc tcttggcttt gaggtgactt atctttccgt
    24361 ggacagtgag ggacacattt ccttagagga actggagcag tccatccgtc ccgatacgat
    24421 tcttgtatct gtgatgtatg taaacaatga aatcggggcc attgagcccg tagaagagat
    24481 cgcatctctg gtacacggaa agggaaaaaa cattattttc catgtggacg ctatccaggc
    24541 ttatggtaaa atagtaatcc ggcccaagaa gcagggaatt gaccttttaa gcgtcagcgg
    24601 ccacaagatc cacgggccaa agggcgtagg ttttctctat atagatgaaa aggtaaagat
    24661 taagcccctt ctttacggcg gcggccagca gagggatctg cgctccggca cggaaaatgt
    24721 tccgggcatt gcgggtctgg ggcttgccgc tcaggagatg taccgggatc atgaggctaa
    24781 aattggaaaa atcaccggtt taaaggatta tttcattgac cgtgcgttgg agcttgagga
    24841 tgtaaccgta aacagccaga agggcggttt aagcgctccc cagatcgtca gcctaagctt
    24901 taagggcgtc cgcagtgagg tgcttctcca cgccctggag gataaggaaa tctatgtatc
    24961 ctcaggctct gcctgctcct ccaatcaccc cgctatcagc ggcaccttaa aggctgtggg
    25021 agtaaaaaag gagcttttgg attctaccct ccggttcagc ttcggccttt ttaacacaaa
    25081 ggaagagatc gactattgca tggatgtatt aaaggagctg cttccggttt tgagacgcta
    25141 ttacagagcg tagctgtcag tttgttcgtt gtttagaaag aggtatatat gcagtatcag
    25201 tcatttttaa ttaaatatgc ggagatcggc accaagggca agaaccgctt tatgtttgaa
    25261 gacgccctga tcaagcagat ccgtttcgcc ctccgggagg tagacggaag ctttgaggtg
    25321 acaaaggaat caggacgcat ctatgtaacc gctgagggcg attatgacta tgatgacacc
    25381 attgaggcct taaagcgggt attcggtatc gcagatatct gccccatggt gcagattgac
    25441 gacagggatt atgagaacct gaaaaaacat gtggtagagt atatggataa ggtctaccct
    25501 gataagaacc ttacctttaa ggtggtggca agaagaggcg acaagcgtta tcctgtgtcc
    25561 tccgaccaga ttaaccgtga tatgggagag gccattcttg aggcgtttcc tcagatgcgc
    25621 gtggacgtgc acaacccgga tgtgctcctg agagtggaga tccgccagaa ggtaaatatc
    25681 ttttccctga tgatcccggg gccgggtggt atgcctgtcg gcaccaacgg aaaggccatg
    25741 cttcttcttt ccggcggcat cgacagcccg gtggcaggct atatgatcgc aaagagaggc
    25801 gtaaagatcg atgcggttta tttccatgca ccgccttaca ccagcgaccg tgcaaagcag
    25861 aaggtagtag atctggccaa gctggtagcc cgctattcag gccccatgga tcttcatgtg
    25921 gtgaatttta cggatattca gctgtatatt tatgagaaat gcccccatga ggagcttacc
    25981 atcatcatgc gccgctatat gatgcggatc gctcaggcta ttgcggaaaa gaacggagcc
    26041 atcggcctga tcacgggaga gagcatcggg caggtagctt ctcagaccct acagtcactg
    26101 gcagctacca atgaggtctg cactatgccc gtataccgtc cggtgatcgg ctttgacaag
    26161 caggagatcg tggacgtgtc agaaaagatc ggaacatatg aaacatccat ccagcccttt
    26221 gaggactgct gcaccatctt tgtggcaaag catccggtga caaagccaaa tatcaacgtg
    26281 atccgcaatt ccgagcgcca tctggaggag aagatagacg agcttgtaaa aacagcgctg
    26341 gagacaacag aggtaatccg ctgtcagtaa aaaaggacga aaaaacgccg cctgccaaag
    26401 cagacggcgt atcctgttaa aagcgctgtt tcatcagtcg atgatgtagg gagccagtgt
    26461 agataagatg tcctcaacac tattttcagc gtggatattt aagtcgatgg gcttggaaag
    26521 atccaggctg aagatgccca tgatggactt tgcatcgatc acatatcttc cggatacaag
    26581 atcaaagtca acatcaaatt ttgccaggtc atttacaaag gatttgacct tgtcaatgga
    26641 atttaaggaa atacgaacag ttttcatatt gaagaaccct ccttgcggta tgtataatct
    26701 gattttatac taaaggagag actcataaaa gtcaatagtg agtttgtatg aaaaaaaccg
    26761 gaacaaactg taaaaagttt aaacggcagg aaaggagatt aagttatgcc gaaagcggcg
    26821 tttcacaatt tgggctgcaa ggtaaatgcc tatgagacag aggccatgca gcagcagctg
    26881 gcacagaggg gctatgaaat tgtccccttt gatgagaagg cagatgtata tattatcaat
    26941 acctgctctg tcaccaatat cgcggaccgg aaatcacgcc agatgctcca ccgggcaaaa
    27001 aagctcaatc ctcaggcact tgtggtggca gcgggatgtt atgtccaggt ggcctcagag
    27061 gagcttaaaa aggatgccag tgtggatctg atcattggaa acaacagaaa agcagagctg
    27121 gcagatattc tggatgcccg tctggcagaa aaaaatgagg aaaacagggt gtatgtgctg
    27181 gatattgccc atacccacga gtatgagcgc cttcatctgg atcgtctggg aggccatacc
    27241 agggctttta tcaaggtaca ggacggctgc aaccagttct gcagctactg catcatcccc
    27301 tacgccagag gaagagtccg cagccgcagc catgaggatg tggcagagga ggtaaaagga
    27361 ctggtagcag gagggtataa ggaagtggtt ctcacgggaa tccacttaag ttcctacggc
    27421 attgagcgca tggaggaaaa tccggtgagc catggcgact ggaaccacag ggagctgctg
    27481 gcgctgataa aggaagttca cgccatcgag gggctggagc gcatccgtct gggatctttg
    27541 gagccaagga tcattaccag agaatttgca gaggagctgg caaagcttcc caagttctgc
    27601 cctcatttcc atctgtctct ccagagcggc tgtgatgaga ccttaaaacg catgaaccgc
    27661 cattatacca cagaggatta tgaggccaga tgccgtctgt taagagaggt ctttgaccat
    27721 ccggccatta caaccgatgt aatcgtgggc tttccggggg aaacggagga agaatttgct
    27781 ctcacaaaag agtatctgga acggatccat ttctatgaga tgcatatttt caaatattcc
    27841 agaagagagg gtacaaaggc agctgtgatg aaggatcaga tccctgaatc cgtgaaggca
    27901 gagcgcagcg acgtgctcct ttctctggaa acgaagatgt ctgaggaata cagaaagcat
    27961 tacggggatt ctgtactttc cgtactgttt gaggagatta cagtcataga cggaaaggaa
    28021 tacctgaccg gctatacgcc tcagtatgtg agggctgccc tgcctgtaac ggacagggaa
    28081 atctgcagcc gcatggcagg aaatatcttt gaagtacagg cggaaggctt tgtaacagat
    28141 gaggctctgc gggtaaaatt gcaggaaata actgtataaa ttaaacaaaa aacagggcaa
    28201 atcaaaaata ctctattgcc gaatggggaa atatagggta gaatatagaa gaatacttaa
    28261 aggacgtgat aatatgggca atataagaga aacacaatat tttcaggctg ttcaggacaa
    28321 aaagatggaa gtgagcgatg tgctggagca ggtgtatatt gccttgacgg aaaagggcta
    28381 taaccctgta aatcagatcg tgggttatat tatgtccgga gatcccactt atatcaccag
    28441 tcataagggc gccagaagcc tgatcatgaa ggtggaaaga gatgagatcc tggaagagct
    28501 gatggctgtt tacattgatg cccgtctgaa ataacagtgc cggtcagact gcggctgcag
    28561 gcggatacag gcagcacttt gctgcttcgg ataaaatacc gtcaggagac tgggaacggt
    28621 ctctttgttt gatgacggta cagtataata caggaatatg aaacggtcat tttcctgctg
    28681 caacgtccat tgaggtcttc taaaatgggg agcctgatgg acgttgtatt gtatatggaa
    28741 ataaagcaga ggaggacgct ttttaagtcc gctcctgctt aaaaatggcg gctgccaaag
    28801 ccgaaatccg gcagctgccg tatagatgtg gaggaattga atatgagaat catgggtctt
    28861 gattacggct ctaagactgt gggagtagca gtctgcgatc ctttaggtat cacagcccag
    28921 accgtggaaa cgatcacccg caaggatgag aataagctgc gccagaccct ggccagaatt
    28981 gaagctctgg ctttagagta cggggtagaa cggatcgttc ttggttatcc taagaatatg
    29041 aacaatacgc tgggagaccg ggctaaaaaa tccgaggaat taaaagaagc cttagagcgc
    29101 aggacaggcc ttcctgtggt gctttgggat gaacgtctta ccacagtggc tgcagagcgc
    29161 gtgctgatgg aaagcggcgt gcgcagagag cacagaaagg aatctgtgga tcagatcgca
    29221 gctgccatga tcttacaggg atatttagac cgcatggcga tggaacagaa aaaggaagaa
    29281 acagccggtg atatgccgga gagcggagaa taagatggaa gaaagaacag actgcattac
    29341 catggtgaca gaggatggag acgaggtggt gttttccgta ttggaggaga ccaggatcca
    29401 tgggaaggat taccttcttg tcacagacgc ccctgaggat gaggatggag aatgttatat
    29461 tttaaaggat gtatccggag aacaggagcc ggatgcggtt tatgagtttg tggaagacga
    29521 cagagaactt gacagtgtaa tggggatttt tgaagccctt ttgtctgatg cagatgtgga
    29581 aattgaaaag taaaaataag gaagaaagat tggaggaatc agtttgagtt tagaagaaaa
    29641 agaaaatgga atcgagcagg aaaataatac gcctgaacca gcagggcata caggagaagc
    29701 cggttcagaa aacagtgcgc cggtaaaaag acgccctcag aaccgccggc cggttcagag
    29761 aagaacggcg agaccgaaaa atacatctga agaaggaagg aaggctgagg gagcggaaag
    29821 tacggtacag acagaaggcg caccggaggc cgcagaaaag aaggaacaga acagacgggc
    29881 tcccagacgc cgcactccgg ctcccagaca gggacagggt accggcagaa gacaggttcc
    29941 ggctccttta agaaatacag atgagacagc ggcagctctt cctgcaaagc gcagcacacg
    30001 gggaacaaaa aaggacggaa aaggcaagtt aaaggtgatc cccttaggcg gcttagagca
    30061 gatcggcatg aatattacag cctttgaata tgaggattct attatcgtag tagactgcgg
    30121 acttgccttc ccgggggatg atatgctggg aatcgatctg gtgatcccgg atgtgaccta
    30181 tttaaaacag aatattgacc gtgtaaaggg ctttgtgatc acccatggac atgaggacca
    30241 tatcggtgct cttccttata ttttacagca ggtaaacgtg ccggtttacg gaaccaagct
    30301 gaccattgcg ctcattgaac acaagttaaa agagcacaat ctgttaaaga ccaccaggcg
    30361 ccgtgtgatg aagcatggcc agtctgtgaa tctgggctgc ttccgggtgg aatttataaa
    30421 gacaaaccac agtattcagg atgcgtctgc tctggcaatt ttttcaccgg tcggcattgt
    30481 gctccacact ggcgacttta agattgatta tacacctgta ttcggcgatc ccatcgacct
    30541 gcagcgcttt gcagagctgg gaaagaaggg cgtccttgcc atgatggcag acagtaccaa
    30601 tgccacccgt cccggcttta ccatgtccga gcgcagcgtg ggaaagacct ttgatgcgat
    30661 tttcgcagag catacgaatc accgcattat cgtggctacc tttgcttcca atgtagaccg
    30721 tgtgcagcag gtagtaaata cggcttataa atatggcaaa aaggtagtag tggaaggccg
    30781 cagcatggta acgatcattg atattgccag tgagctggga tatatcaata ttcccgacgg
    30841 aacgctcatt gacattgacc agctgaaaaa ctatccgcct gagtccaccg tgctgatcac
    30901 cacaggaagc cagggcgagt ccatggccgc tctgtcccgc atggcagcca gcatccataa
    30961 gaaggtgtcc attatgccgg gagatgtggt tgtattaagt tccagcccta ttccgggaaa
    31021 tgaaaaagca gtggcaaatg tgatcaatga gctttccacc aagggagcca aggtgatctg
    31081 tcaggatact catgtatccg gccatgcatg ccaggaggat ttaaagctga tctactctct
    31141 ggtacatccc aagtttgcca ttcccgtaca cggcgagttc cgtcacaggg ttgcgcagaa
    31201 ggaactggca gagtatatgg gcgttgccaa ggataacgcg atcctggtta attcaggcga
    31261 tgtagtggct ctggatgagg acagctgtca ggtgatcgac catgtgacag caggcggtat
    31321 tctggtagac ggtctgggtg taggcgatgt gggaaatatt gtcctgcgtg accgtcagaa
    31381 tctggctcag aatggtatta tcgtggtagt gcttacgctg gagaagcatt ccaaccagct
    31441 gctttcgggc ccggatattg tatccagagg ctttgtctat gtgcgggagt ctgaggatct
    31501 tttggaggaa gctcataata tcgtggcaaa tgcagtagag gactgcttag accgccgcgt
    31561 aagcgactgg ggcaagatta aaaatatcat caaggacagc ttaagcgagt ttttatggaa
    31621 gcgcatgaag cgcaatccta tgatccttcc aattattatg gaagtataag cctttgatat
    31681 acagcaggag gagcgacagg ccatgagcag acggacaagg gagatcaatc gaataaccac
    31741 agccattatc accatttcca ttaagcttat ggtgctggct cttcttatcc tgcttttata
    31801 cgaggccgta gtccggggat ttgcctttgg ccatgagatt ttttatgcag aagcggcaga
    31861 ggcggctccg gggagagaaa tcacggtcga gataaaagag ggagagacag caaaagaggc
    31921 cgcgggagaa ctaaagaaaa agggactgat cacgaatgat ttcgcattct ggtttcagag
    31981 tattttctat gattaccagg acatttatcc cgggatctat acgcttaaca cctccatgac
    32041 ttccaaggag attttaagag agctgaatgt aaaacctgag gaggaaagcg aagcgtcccc
    32101 cgaagcccgc acggtgagcg gggaggcgga tctctcaaaa gatgcagatt cctcagagaa
    32161 caggaagacc agagcggttc aggcagagcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    32221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    32281 nnnnnnnnna tatctatatg gaggaagagg aatccggcgg ctggatcgaa gaggcagagg
    32341 aataagacat gattgtaaat gacagaatca cagactatat ccagtccctg gaaaccagtc
    32401 agggaccggt tttggatgaa atagaaaaag aggctctgga tcagtttgtg cctatcatca
    32461 gaaaggagac ggcagctttt ttaagaacca tgacggcagc ccttaagccg tcggccatac
    32521 tggagatcgg cacggccgtc gggtattctg ctttgcagat gtgccgggtg atgccggagc
    32581 actgccgcat tactaccatt gaaaagtatg agaaacgcct tcccatcgcc agagagaatt
    32641 ttcatcgggc aggagaaagc ggacggatca cacttttaga gggcgatgca gacgtgcatc
    32701 tgaaggagct ggcaaaaaaa ggctgtcagt ttgatcttgt gtttatggat gcagccaagg
    32761 ggcagtatct aaactggctt cccatggtgc ttgatcttat gcccaaaggc gcggtgctca
    32821 tttccgacaa tgtgcttcag gacggcgata tcatccagtc acggtttgcc gtggagcgca
    32881 gaaaccgcac catccacagc cgcatgagag agtacctgta tgagttaaag cacacagagg
    32941 aactggaaac ctccgtgatc cccattggag acggagtgac agtcagtaca aaggttagat
    33001 aggagaacga tatgagagaa acagagctgc tgataccggc aggcagtctg gacgtattaa
    33061 agactgctgt ggtttacgga gcagatgccg tatacatcgg cggagaggtc tttggcctca
    33121 gagccaaagc gcacaatttt tccaaagaag aaatgaaaga gggaattgcc tttgcccatg
    33181 agcgcggggt caaagtctat gtgactgcca atattctggc gcacaatgac gatctgccgg
    33241 gggtagaggc ttattttgaa gaattaaaag aagtggggcc ggatgccctg atcatttcag
    33301 atcccggcat ctttgccatt gcaaagagag ttcttcccca gatggagctc catgtgagca
    33361 ctcaggcgaa taataccaat tacggcacct atttattctg gcatcagctg ggagcgaaac
    33421 gggtagtttc tgcaagagag ctgtccttag aggagattaa ggagatccgg gcccatattc
    33481 cggaggatat ggagatcgag agctttatcc acggagctat gtgcatttct tattccggaa
    33541 gatgcctttt gagcaatttc tttaccggac gggacgccaa ccagggggcc tgcacccatc
    33601 catgccgttg gaaatattcg atcgtagagg agaccagacc gggagaatat atgcctgttt
    33661 atgaaaatga gcgcggcacc tatatcttta attccagaga tctgtgcatg atcgagcata
    33721 ttccggagct gatcgatgcg ggagttgaca gctttaagat cgaaggccgt atgaagacag
    33781 ctctttatgt ggcaacggtg gcaagaacct atcgaaaagc cattgatgat tataaaaagg
    33841 atcctgccct ctatgagcag aatatggagt ggtataagga agaaattgga aaatgtacct
    33901 acagagagtt taccacaggc ttttattttg gaaagccggg agcagatgcg cagatttacg
    33961 acagcaatac ctatgtaaaa aattacacct atctgggaac ggtagaggag tccgaaggct
    34021 gccggttcag aatcgagcag aagaataagt tctctgtggg agaaaccatt gagatcatga
    34081 agcccgatgg acgaaacgta caggcccttg taaagagtat ctgcgatgaa gagggcaatg
    34141 ctcaggagag cgctccccat cccaagcaga tcttgtgggt ggagctggat gcgccggcag
    34201 atgtgtatga tatactgaga aagaaagcgg agcagtaaaa gacaggaggc ggtaagtttg
    34261 gtggatggta tcctgctgat cctggcagca ttgtgtgcgg tttactttgt gatcattgtg
    34321 atctatgggg gaatcggcac ttcttttgcc tttatatggc tgttttttgc ggctctttgc
    34381 gtatttcttg tttatggaaa atggtattac agccgtcata tgtttgacat tcccaagtgg
    34441 gtgcctgtga gtatcgttac cacctgtgtg atgggagtga ccgtcatggc aatgatatgt
    34501 actctggtgt ttttgggcgt cgcttccgag gaagaacccg gacttgatta tgtgatcgtt
    34561 ctgggagcta aggtaaagga acatacggtg agcaattcac ttaaaaaacg gctggataag
    34621 gccattgact atgcccagaa aaatccggaa acctatctgg tgctttcagg gggaaaggga
    34681 gcggatgaac ccataagcga ggctcaggcc atgtacaaat atctggtcta taacggtgtc
    34741 cgtccagagc agctgctcct tgaggatcgg tctgccagta cggtggaaaa tattgcctac
    34801 agcagacttg tgatcgaagg ccatcgggaa cggctgcgtc tggaacggga gcagcaggag
    34861 aaaaaacgca gggaacctta ccttatggta gatgacaagc ctgtggaaat cggtgttctc
    34921 accagcaatt tccatgtatt cagagccagg atgattgccc gcaaatgggg ctttgagcat
    34981 gtttatggca tcagcgccca gtcggatcct gtgcttttta tccatctgtg cgttagggaa
    35041 tgtgcgtcga ttataaaaga ccggcttatg ggaaatatgt aggatagaat gactgcttaa
    35101 aggaggactg aaatgattga caaaaaaatg ttagaccgcg taaaagagct ttcacagtac
    35161 cgtcttgtag atgaaagacc tgttgaggag atgaactcat atggaatggt gctggagcat
    35221 aaaaagagcg gagccaggat ttttttaatg agctgtgaag atgacaataa agtattttcc
    35281 attggattcc gcactccgcc ccaggacagc acaggacttc cccatattct ggaacatagt
    35341 gttttggagg ggtctgagaa gtttcccgta aaagatccct ttgtagagct ggtaaagggt
    35401 tctttaaaca catttttaaa tgcaatgacc tatccggata agacggttta tccggtcgcc
    35461 agctgcaatg acagggattt ccagaacctg atggatgtgt atttagacgg cgtgctccat
    35521 ccttccattt accgtgaacc gaagattttt ttacaggagg gctggcacta tgagatggag
    35581 gagacagaca gccccttaat catcaacggc gtggtttaca atgaaatgaa gggcgctttt
    35641 tcctcaccgg aaagcgtgct ggaccggtat acaaaggcgg ttctctttcc ggatacccca
    35701 taccgccatg aatccggcgg cgatcccgcc tgtattccgg atctgaccta tgaacagttt
    35761 attgccttcc ataaaaatta ctatcatccg gccaacagct ttatctacct ttatggagat
    35821 atggatatgg ctgaaaaatt agagtggctg gacagagcgt atttatccca atatgacagg
    35881 acagatttta ccctggattc ccggattccc atgcaggagc cctttaagga gcccattgag
    35941 agggaaacta cctactctgt aacagcggaa gaagggacaa aggggcgaac ctatctgtct
    36001 ttaaataccg tagtgggaac ggatctggat ccggagcttt atgtggcttt ccagattttg
    36061 gaatatgcgc tgatcgatgc tccgggagca cccttaaagc aggcgcttat tgacgcccat
    36121 attggtcagg atattctggg aggctacgac aacgggatcc ttcagccctg cttttccgta
    36181 atcgccaaaa atgcagagcg ggagcagaag ggggaatttc ttgctgtggt taagggaacc
    36241 ctgcgccgtc tggcagatca gggcattgac agaaagagcc ttcttgcagg cttaaactac
    36301 tacgaattcc gctacagaga agcagattac ggctctgcgc ccaagggact gatgtacggt
    36361 ctctggtcca tggacagctg gctctatgac ggagatccca tgctccatct ccagtatcag
    36421 gagacctttg actttttaaa gaaggcagta tccgaaggat attttgagaa tctgatccgc
    36481 ggatatcttc tggataatcc ccatgaggct tttgtgatcg taagcccttg tacagatcag
    36541 accgccagag aggatgaggc tctggcagag cggcttaagg cttacaggga ttccctttca
    36601 gaagaagaga ggagcgagat tgtccgaaag acaaaggaat taaaggcata tcaggaggag
    36661 ccgtcctctc aggaggatct ggaaaagatt cccatgctcc agagatccga tattgagaaa
    36721 aaggcagaag gcttttctta tgaggtaaag gaggagagcg ggattcctgt catccacagc
    36781 ccgttattta cctctgggat cggctacctg aaggtgctct ttgacttttc tgtcctgccg
    36841 gatgaggata ttccctacgg agctctttta aagtccgtgc tgggctatgt ggatacggag
    36901 cattacagct acagcgatct taccagcgag atttatttaa acagcggcgg cctggatttt
    36961 tccgtaggcg gcagccagaa aatgggagag cctggaaact ttaccggtac ctttacagcc
    37021 agcgtaaagg tgctctatga aaagctggat tttgcttttg agatgctgga agagatcctc
    37081 ctgcgctcta agcttacaga cgaaaaacgt ctgggagaga ttttggatga gactatgtcc
    37141 agagccagaa tgcgtctgga gaacagttct cacagctccg ctgccatgag agctgcttcc
    37201 tattattcgc ccctgtcttc tttctatgac cgcaccggcg gtattggctt ttatcagttt
    37261 ttggagaaaa ttacagagcg ctgcagcaag gaagagggat atcgtctcaa actggggaaa
    37321 aagcttatgg aagttgcaaa aacgctcttt actgcttcca atatgaccgt aagctacacg
    37381 gcggatgaag agggctatgg caggcttccg gctgcgctgg ctgcctttaa ggcggttctt
    37441 ccggaaggga atgggatccg ttacccctat gaatttaagc cagagcttaa aaatgaaggc
    37501 tttcggactg catcgcaggt caactatgtg gcctgctgcg gcaccttcgg agagagtgct
    37561 tatacaggcg cattaaaggt attaaaggtg attttaaatt acgaatatct ctgggtaaac
    37621 ctgcgtgtaa aaggcggagc ctacggctgt atgagcagct ttgggcgaaa tggggaaaca
    37681 atgttagtat cttaccggga tcccaagctg ggcgagacaa accgggttta tgagggaatc
    37741 ccggagtatt tgagaacttt ttccattgac gacagagata tgacaaaata cgttatcggc
    37801 gccttcagtg agctggatgc tcccttaaat cctgcggcaa aaggagccag aaacctggga
    37861 gcatggcttt ctggcgttac ggacgagatg atccagagag agcgggatca ggttctgaat
    37921 gtgacccagg aggatatccg agcactggcc ggactgttag agtctgtgtt aaacacaggc
    37981 gctctctgtg ccattggaaa tgaccagcag attgtgaatg aacaggaact ctttaacgaa
    38041 ataaagcatc tttaccacta aatgagcagg agcagaaatg atggaagaaa atgtacagaa
    38101 gaaatcaggc tttgtcacac tgatcggccg tcccaacgta ggaaaatcta cgctgatgaa
    38161 tcatctgatc ggccagaaga tcgccattac ctctgataag ccccagacca ccagaaacag
    38221 gatccagacg gtgtatacag atgaaagagg ccagatcatt ttcctggata ctccgggaat
    38281 ccacaaggcg aaaaacaagc tgggcgaata tatggtagat gtggcagagc acaccttaaa
    38341 ggaagtggat gtgatcctct ggctggtgga acccaccacc tttatcgggg ccggggaacg
    38401 ccacattgca gagcagcttt ccaatgtgaa aacaccggtg attctggtga tcaacaaaat
    38461 tgatacctta aaaaagcagg aagaaatttt aacatttata gatgcctaca aggatgtgtg
    38521 caagtttgca gaaattgtgc ctctttccgc actgaaggaa aaaaataccg atcttttgct
    38581 ggagcttctt tttaaatatc tgccctgcgg ccccatgttt tatgatgagg acacagttac
    38641 ggatcagccc atgcgccaga ttgcggcaga gcttatcaga gaaaaagcgc ttcgccttct
    38701 ggatgatgag attccccacg ggatagcagt tactatcgaa aagatgcgcg aaaggaaaaa
    38761 cggcatcatg gacattgaag ccagcattgt ctgcgaacgc gagtcccaca agggaatcat
    38821 tatcggaaag ggcggctcta tgttaaagcg catcggaacc aatgcccgca gggaaattga
    38881 aaatatgctg gagatgcagg tcaatctgca gctgtgggta aaggtacgca aggaatggag
    38941 agacagtgag ctgtatatga aaaattacgg ctatgacaaa aaagacgtat aaggggagcg
    39001 agcagggtga gagaagcagt tagcctcacg ggcatggtgc ttttggctgc atcctttggg
    39061 gaagcagacc ggagagtggt gcttttgacc agagaacggg ggaagatcac agcctttgct
    39121 cacggagcaa gaaggccggg aagcgccctg atggcagtta ccagaccctt tgtgttcggc
    39181 acttttaacg tctttgaggg ccgcagcgcc tatacccttc agagcgccca gccggtatgc
    39241 tattttgacg agctttctgc agatatggag ggggcctgtt acggagctta ttttctggaa
    39301 atggcctcct atctggctca ggaaaacatg gacggaaccg aacttttaac actggtttac
    39361 cagacattaa aggccctttt aaatccgtcc cttccaaacc gcctgatccg gcggatcttt
    39421 gagctgaaag tcatggttat aaacggagaa tacacagaaa atccccaggg aaaggtatcc
    39481 gagtcctgta cctacacctg gcaatatgtc atttattccc ctgtaggaag cctctatacc
    39541 ttcgtactaa aacccgaagt ccaggccgaa ttcgagcacc aggtagaaaa cgccagaacc
    39601 accttcatcc gccacaactt ccgttctctg gaaattctgg aactgatgac caggtagaac
    39661 agatggaaat gttttggttt tacctataga aacaacctat ggccattggc gaccacaaca
    39721 aatacttgcc gataaggctt gaaattatac gcatcagaag atataataag cggtatattg
    39781 ctcccgcgga ttcaggagaa gcctggaaga aaggaagaaa tatgaagaaa agcataagag
    39841 gaatcctgct tcttgcagcc tgcaccgtct tttttgccgg ctgtgccaga tccaaaggga
    39901 gctgggaagc ctttacggaa aatacccttt atgtagccgg cgatggaagc gtttcctggg
    39961 cttctgtcga aagcttcggg gaaggcgatt acaaagaaga ggagttaaaa gcctttgccg
    40021 gagagagaat cagccagttt aaccagtctc agggcgaaga gccagccttt gaaaatgaga
    40081 agggggcaga aaagcttccg gttgctcttg atacagtgag tatgaaggat ggaagggcag
    40141 ttctggtgac ggaatacgat accccttccc ggctggtgga gttttcacag gacataggag
    40201 atgaggggat gccctttgat tcgattgata taggcagtcc tgcgtccctg gctttgaaag
    40261 aacagacttc ttatctggat acagagggaa aagcggcaga tcagcaggaa attttaaagg
    40321 aaacaggtct tgcggttcgt gtttcgggcc agggggtcat ttctgcggaa aaaaagatcc
    40381 gctatataag tgagggctgt acccttcggg atgttcatac agtggaaacc gcgtctgagg
    40441 gagtaagctg cattatattg gagtgatgcc ctgaaagggg cttgctgaca gtaagtatat
    40501 aaaaagagag gacatggatc atggaaaaga caatggaaaa aatcgtagct cttgccaaga
    40561 acagaggttt tgtataccct ggttctgaga tctacggcgg tcttgcaaat acctgggatt
    40621 acggcaatct gggtgttgag cttaaaaaca atgtaaagaa agcctggtgg cagaagtttg
    40681 ttcaggaaag cccatacaac gtaggagtgg actgtgcgat tctgatgaat tcccagacat
    40741 gggtagccag cggccatctg ggcggatttt ctgatccatt gatggactgt aaacagtgta
    40801 aggagcgttt ccgcgcagac aagctgattg aggattataa tgatgagcac ggtatcgaga
    40861 tcgaaggctc tgtagacggc tggtctcagg aagagatgaa gcagtatatt gaggataagc
    40921 acatctgctg tccaagctgc ggcgctcacg attttacaga tatccgtcag ttcaacctga
    40981 tgtttaagac cttccagggc gtaactgagg atgctaagaa taccgtatac ttaagacccg
    41041 agactgcaca gggtattttc gtaaacttta agaatgttca gagaacctcc agaaagaaag
    41101 ttcccttcgg tatcggacag gttggaaagt ccttcagaaa tgagatcact cccggaaact
    41161 ttaccttccg taccagagag tttgagcaga tggagcttga atttttctgc aagccgggca
    41221 ccgatctgga gtggtttgca tactggaagc agttctgtat tgactggctt cagacccttg
    41281 gcatcaagcc ggaggagatg cgtgcaagag atcactcacc ggaggaactg tgcttctaca
    41341 gcaaggctac cacagacctt gagttcttat tcccctttgg ctggggtgag ctgtggggta
    41401 ttgcagacag aaccgattat gacctgaccc agcatcagaa tgtatccgga caggatatgg
    41461 catactttga tgacgagagc aaggaaaaat acattcctta tgtaatcgag ccttcattgg
    41521 gtgcagaccg tgttacactg gcattcttat gtgctgctta tgatgaggaa gagatcggag
    41581 agggcgatgt gagaactgtt cttcatttcc atccggcgct ggctcctgta aagattggtg
    41641 ttctgcctct ttccaagaag cttaacgagg gcgctgagaa ggtatttgca gagctttcaa
    41701 aatactataa ctgtgagttt gatgacagag gaaatattgg aaagcgctac agaagacagg
    41761 atgagatcgg tactccgttc tgtgtaacat atgattttga ttctgagaac gatcaggcag
    41821 ttactgtccg cgaccgcgac accatggagc aggtacgcgt accgattgct gagttgaagg
    41881 cttactttga ggataagttc cgtttctagg gaatatgtaa aatgaaagaa aacagcatct
    41941 gggaaaccgt taaaagcggt atttccgggt gcttttttca tatgcctttt ccaagcggcg
    42001 cgtagaaaag tttagaatga ttttataaac gccccgccat aagtatggta tacttaaact
    42061 atatgtaaat tgcctgtctg cgtgctgcgc cgggggcagt ggaaggagga tgaaaaggtg
    42121 aagataagga cacggcttgc ggtagcattt attatcataa ccgtttttcc gatgcttatg
    42181 atctaccttt ctgtgatcgc cctcagcgat tatcaggaaa agtcctttaa agaagcatat
    42241 ggtcttacag agcagattga actgttaaac ggcaattccc ttcaggtatt taaccgtttg
    42301 accagacagt cgcagaaaga tatccgaaag gtgttggatg aaaacccgga taattacaag
    42361 gatccggcat atctggataa agtgaatgca aaattaaatg aacagtatgc ctatctgatt
    42421 gtccgccgtg aggatacgat catttattac ggggcgaacg gagaaccgca ggatgcgtct
    42481 ctctgcgctc agcttccgga ttttgatgtg atgaagggac gcatggaagg tgggatctat
    42541 ctggacggcg agagtcagca tctgatcaag cagatggatt ttcagttttc ggacggtgcc
    42601 gagggaagtg tgtttattgt ttccaatgta gacgatctgc ttcctgaagt aaagtccatg
    42661 tttcgggaaa tgctgttttt gggcgtggcg attctggtgg tagcgggctt tacccttacg
    42721 gtttgggtat accgttcgat tttaagcccc ttaaacaagc ttcaggaggc taccaagcag
    42781 atacgggacg gtaatctgga gtttaccctg gatgtagatg cagatgatga gataggccag
    42841 ctttgccagg attttgaaga aatgcggatc cgtttaaagg aaaatgcaga ggaaaagctt
    42901 cagtatgata aggaaagcaa ggagctgatc agtaatattt cccatgatct taagactccc
    42961 attacagcca ttaaggggta tgtagaaggg attctggacg gggttgcttc ctcacctgag
    43021 aagctggaca gatatatccg cacgatttat aataaagcca atgatatgga ccgactgatc
    43081 gatgagctga ccttttactc caagattgac acaaataaga tcccttatac ctttgcaaag
    43141 atcaatgtat cccagtattt taaggactgt ctggaagagg tgggtctgga tatggaagca
    43201 aggggcattg agctgggata tttcaattat gcggatgagg atgtgatggt cattgcagat
    43261 gcagagcaga tgaagcgtgt gatcaataac attatcagca attccgtcaa atatctggac
    43321 aagaaaaaag gaatcatcaa tatcagaatc aaggatgtgg gagactttat ccagattgag
    43381 attgaggaca atggaaaagg cattgcggca aaggatcttc ccaacatatt tgaccgtttt
    43441 taccgtacag attcctcacg aaactcttct cagggcggca gcgggatcgg actgtccatt
    43501 gtacgcaaga tcattgagga tcacggagga cgcatctggg ccaccagcaa ggaaggcatc
    43561 ggaacggaga tccattttgt attaagaaaa tatcaggagg ttatgcagga atgagcaaaa
    43621 tactgattgt agaggatgaa gaaagcatcg cagagctgga aaaggattat ctggagctgt
    43681 ccggctttga ggttgagatc gaaaataacg gaaccgatgg tctggaccgt gctttaaagg
    43741 aagactacga tctcctgatc ctggatctga tgcttccggg aacggacgga tttgagatct
    43801 gccgcagggt aagggagatt aaaaataccc ctattattat ggtttctgcc aaaaaggagg
    43861 acattgacaa aatccgcggt ctgggccttg gagcagatga ttatattaca aagccgttca
    43921 gtcccagcga gatggttgcc agagtgaaag cccacatggc ccgctatgag cgtctgatcg
    43981 gaagcggcca gcccgccaat gagattattg aaatccgcgg tttaaagatc gacaaaacag
    44041 cccgcagagt atgggtggca ggagaggaaa aaacttttac aaccaaggaa tttgatcttc
    44101 taaccttttt agcccagaat cccaaccatg tatttacaaa ggaggaactg ttcagcaaaa
    44161 tctgggatat ggaatccatc ggcgatattg ccaccgttac agtgcatatt aagaagatcc
    44221 gggaaaaaat tgaatttaat acagccaagc ctcagtacat tgagaccatc tggggagttg
    44281 ggtatcggtt taaggtatag ggaaaatcct ccgcttgtgc gggggatttt tcatatgcag
    44341 aaaggaatgg cacatttctt tgttaatagt tactataaaa tattagttcg catatacgaa
    44401 caataaatga atatacgaaa atgacagaaa atgaaaacgc ttacagtaaa ttttgttgta
    44461 aaataaccaa attttttcaa aattttgtcg gtaaaggact ttactccccc aatctttgtg
    44521 atataataaa cgaggtggac ggataaggag aaccattgat tccgtctgat aggcgggcac
    44581 aggaaatttc ccggtatgtc cggttaaaga gcggcagagt cctgagggaa tctgccagaa
    44641 tgaggaggaa gaaacttatg gctacaaagt gggtttattt attcagcgaa ggcaatgcga
    44701 ccatgagaaa cctgttaggt ggtaagggcg ctaaccttgc ggagatgaca aatctgggat
    44761 tacctgtacc acagggattt acgattacaa cagaggcctg cacccagtat tatgaagatg
    44821 gcagaaagat caatgacgag atcatgggcc agatcatgga gcatattgga aagatggagg
    44881 agatcaccgg caagaagttc ggcgataagg agaatccgct gttggtttcc gttcgttccg
    44941 gtgcaagagc ttccatgccg ggtatgatgg ataccatctt aaacctgggt ctgaatgagg
    45001 aagtagtaga ggttctggct gcaaagtccg gtaatccgcg ctgggcatgg gactgctaca
    45061 gaagattcat ccagatgttt tccgacgttg ttatggaagt tggaaagaag tattttgaag
    45121 agttgatcga taagatgaag gctgagaagg gagtaaccca ggacgtggag ctgaccgctg
    45181 aggatcttca cgagctggca aatcagttca aggctgaata taaagaaaag atcggaaagg
    45241 atttcccgac caatcccaag gagcagctga tggaggccat caaggctgta ttccgttcct
    45301 gggataaccc aagagcaaac gtataccgcc gcgataatga tatcccttat tcctggggta
    45361 ctgctgtaaa cgtacagatg atggcattcg gaaacatggg cgagacttct ggtacaggcg
    45421 ttgcatttac ccgtgaccca gctaccggcg agaagcacct gatgggcgag ttcttaatga
    45481 atgctcaggg tgaggacgta gttgcaggtg tccgtacccc tcagaagatt gaccagttaa
    45541 aggaagttat gccggaggtt tatgagcagt tcgtaggcat ctgccatact ctggaggatc
    45601 attacagaga tatgcaggat atggagttca ccattgagga taagaagctt tatatgctgc
    45661 agaccagaaa cggaaagaga acagctaagg ctgctttaaa gattgcctgt gatctggtag
    45721 atgagggcat gatcgatgag aagaaggcag ttcagatgat cgatccccgt aatctggata
    45781 ccctgcttca cccacagttt gatgcagagg cattaaagaa ggctgagccg gttgcaaagg
    45841 ctctggctgc atccccaggc gctgcctgcg gcaagatcgt atttactgct gaggatgcaa
    45901 aagagtggaa ggaaagaggc gaaaaggtcg tattagtacg tcttgagaca tctcctgaag
    45961 atatcgaagg aatgaaggct tctcagggta tcctgacagt ccgcggcggt atgaccagcc
    46021 acgcagctgt agttgcccgc ggtatgggta cctgctgtgt atccggctgt tccgagatta
    46081 tcatggacga ggcaaacaag aagttcgttc tggccggaaa agagtaccat gagggcgact
    46141 ggctgtctat cgacggttct accggtaaga tctatgacgg cattattcct acggtagatg
    46201 ctaccatcgc aggcgagttc ggccgcatca tggcatgggc tgacaaatat agaagactga
    46261 gagtccgcac caatgcagat actccaagag acgctgcaaa ggcaagagaa ctgggcgcag
    46321 agggtatcgg cctttgccgt acggagcata tgttcttcga gggcaaccgt atcgacgcat
    46381 tccgcgagat gatttgctcc gagacagtag aagagagaga agcagcctta gagaagatcc
    46441 ttcctgagca gcagggagac tttgagaagc tttacgaggc tttagagggc aatcctgtta
    46501 ccatccgttt cctggatccg cctctgcatg agtttgttcc taccgaggaa gaggatatca
    46561 agaagctggc agaagcacag ggcaagagcg ttgagaccat caaggcaatc tgtgattctc
    46621 tccatgagtt caacccgatg atgggacatc gcggctgtcg tctggctgtt acctatcctg
    46681 aaatcgcaaa gatgcagaca aaggctgtca tccgtgcagc aatcaacgta aagaaggctc
    46741 acgctgactg gaacgtttgc ccggagatca tgatccctct gatctgcgat atcaaagagt
    46801 taaagtttgt taagaaggta gttgttgaga cagctgatgc tgagatcgca gcatccgaca
    46861 ttgacttaaa gtatgaagta ggtaccatga tcgagatccc aagagcagct ctgacagctg
    46921 acgagatcgc aaaggaagct gagttcttct gctttggtac caatgacctg acacagatga
    46981 cctacggctt ctcccgtgat gatgcaggca agttcttaaa tgcttactat gatacaaaga
    47041 tctttgagaa cgacccattc gcaagactgg atcagaccgg cgtaggaaaa ctgatggaaa
    47101 tggcgatcaa gttaggcaag agcacccgtc ctgatatgca tgtaggtatc tgcggcgagc
    47161 atggcggcga tcctacttct gtagaattct gccacaagat tggtctggac tatgtgtcct
    47221 gctcaccatt ccgcgttccg atcgcaagac tggcagcagc tcaggcagcg attgccaatg
    47281 caggaaaata gaaaatcgca agtatttcta cttcacaacg ctggttgtta caaggaaaga
    47341 tgtgcaagaa tatatgaaac atcattctga gtatacaaag gcgaaccgat ttaaagacat
    47401 tatttgcaaa atctatatat aactcgaaag ggaagcattg ttttggcttc cctttccttt
    47461 ccttatatct caattagggc taaatcctaa aagctatggc tttaaaggac ttagtccttt
    47521 ttatatgcaa aaaattaaaa taaggagtga tattgtcaat attggtggcg gtcttccagt
    47581 cactcagttc aaagcgggta gcaggtaatg gcgaaagtaa tggcttttca tcttcaagga
    47641 ataagtccaa ccggctaccc tctttctttt gaaagagctt tcgattgaat tgttccagtt
    47701 tttctctgat tacacgattt aattcgacaa gggaaaagaa ctgctcattc ctaagtgctg
    47761 ctgttatcca agtagagata tttcctaccg ttccttcagc attcggctta tcctttggtg
    47821 ttctgacacg agcagggatg atagctgtac catagtgctc agccatttcc tgataagttt
    47881 cattgatctg ctggtctttc catccaccat tatggacaac tgcagtcttg cagttatccg
    47941 gtacaagtat tctggcaaca ccgccaaaaa actcgtacat atggacatgt gctgtaatcc
    48001 atgatttctg cttcatatcc agaaatgctt ctacatatgc atactggctg taagtcatta
    48061 cgccaacgaa tatgtaaacc tttaagattt caccggtatc tgggtcaatg attgttgcag
    48121 gatcccctgc ccagtcaact tcaacctgct caccgggttt gcgattgatg tgcatggtag
    48181 cgcgtcgttt ctgttcatcc tgttggatgt gatagcagaa ttgtgaatac ataagcggtt
    48241 catcgccgtt agcacggcag tcctccatgt attccgtcca cagaagcttt ttactgactc
    48301 cgttgcggag caattctttg tggatgtagt cgtagtcagg catgcgtttg tttttcgata
    48361 ccaaactttc cttagcaaac ataagtcttt gaagttcagt atccgtcata gattcatcaa
    48421 gaggccaaga aagatttaat tctctggcac gtttttgaac cttgactaca gttttctgag
    48481 caacaccaca gcttgccatg atgttacgnn nnnnnnnnat gatttctcga tatttggtca
    48541 taatcgtgac ctccttgtaa tgaatttaca ccatagggtg catatcttca ttataaggat
    48601 ttagtaataa aatgatttcc tttttgacag aatcgtgatt tcctgcaaaa cgtaatggtg
    48661 atttccaagc accggacaag tgatggattt taccccggat tattcaactt aatcagcatt
    48721 tttgatggca aaaggcgaaa agcagttata cagagtattt tagaggtaaa gaaatatttg
    48781 gatgatatgg aaatgttgga agtggtgtca agagtacttg aaaaattgga cagtatgtca
    48841 gatgaggaat ttgaggcaat ggaattcgta gcggcagaat aaaaacagaa tttacgatac
    48901 aaataaaatg tagtataatt gaaatagaaa ataaaattta aaattaagca atctggaggt
    48961 gtataatagt gaatgataaa ggcgtaggtg ctattttttg tttgatatca tcaattttaa
    49021 tttcagcaaa atacttatca gcggcaatat ttgtgagtaa tacatctaat aaggatgcaa
    49081 ttttattttc tgcgagtttg gaatatgtgg gttctttttt gacaatagct tctttgattg
    49141 cattgataat tggacttctg tttttgggat tgaaaattag tcgttctttt ttctatgggt
    49201 atacatgata aactgcggag ctgaacagca aaatatagaa atttgctgtt cagtcctgtt
    49261 tattctttac ttaaatctaa tatttgttca tcaatctaag cggtttacac agaatgattt
    49321 agaatatttc tttcttattt ataataactg gcgaggttaa ataatttaag aaaagga
//
LOCUS       DBMDUH010000005        36860 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_935_length_36860_cov_6.846378, whole genome shotgun sequence.
ACCESSION   DBMDUH010000005 DBMDUH010000000
VERSION     DBMDUH010000005.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 36860)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 36860)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36860
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_935_length_36860_cov_6.846378"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            1..104
                     /locus_tag="ACZPCG_01175"
                     /pseudo
     CDS             1..104
                     /locus_tag="ACZPCG_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004842833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            complement(168..599)
                     /locus_tag="ACZPCG_01180"
     CDS             complement(168..599)
                     /locus_tag="ACZPCG_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HIR1283717.1"
                     /translation="MKISTRGRYALRMMVDLAQHYDQGFIALKDISKRQDISKKYLEQ
                     IIPFLNRGNLLSATKGHMGGYRLAKAPSEITVREILESAEGSLVPVSCMDNTPNLCKK
                     CEDCITLPIYEGLYDVVTKYFDSITLEDIINRQVSEFIYTI"
     gene            752..1690
                     /gene="cysK"
                     /locus_tag="ACZPCG_01185"
     CDS             752..1690
                     /gene="cysK"
                     /locus_tag="ACZPCG_01185"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013280485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HIR1283718.1"
                     /translation="MADIRKSSLELIGGTPLLEAGRYSKKAGVNKAVILAKLEYLNPA
                     GSVKDRIALAMIEDAEKRGILKPGATIIEPTSGNTGIGLAAVAAAKGYRAILTLPDTM
                     SIERRNLLKAYGAELVLTEGSKGMKGAIAKAEELNREIEGSVILGQFINPANPKIHKE
                     TTGPEIWKQTDGKVDIFVAGVGTGGTITGVGEYLKEQNPDIQIVAVEPAGSPVLSKGT
                     PGAHKIQGIGAGFVPQTLNTEIYDEVITIENEDAFKEGRAFATSEGILVGISSGAALK
                     AAVILAGRQENAGKVIVALLPDSGDRYLSTALFSTN"
     gene            1707..>1995
                     /locus_tag="ACZPCG_01190"
     CDS             1707..>1995
                     /locus_tag="ACZPCG_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003503567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1283719.1"
                     /translation="MTLTQLQYVVAIAETKSMNKAAAALFVSQPALSGAIKDLEEELQ
                     TELFIRTNKGIVITTEGQEFLSYARQMVELGNLVEERFVNKTIAKKKFSVSM"
     assembly_gap    1996..2005
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2006..2693
                     /locus_tag="ACZPCG_01195"
     CDS             <2006..2693
                     /locus_tag="ACZPCG_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HIR1283720.1"
                     /translation="IYGQYESVVEKYSEGGSYKKKFGVSTQHYSFAVEAFIKLAKNFS
                     IGEYELAVHETKTHEVIDNVKDYRSELGVLYLNSFNEKAMTRILEDNDLEFMPLYDCG
                     ISVYLSKNHPLAEKTKITMDELSEYPCLSFEQGERNSFYFAEEVFSTHAYKQIIKADD
                     RATMLNLMTGLNGYTLCSGIICQDLNGDFYVSVPLDTTDTMTIGYIKRKNIPLSLLGR
                     KYIDILKSYK"
     gene            2848..4128
                     /locus_tag="ACZPCG_01200"
     CDS             2848..4128
                     /locus_tag="ACZPCG_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HIR1283721.1"
                     /translation="MSKYRFETLQLHVGQEHPDPATDSRAVPIYQTTSYVFRNSQHAA
                     DRFGLADAGNIYGRLTNSTQDVFEKRIAALEGGSAALALASGAAAIAYTIEALAANGG
                     HIVAQKTIYGGSYNLLAHTLTQYGITTTFVDAHNLEEVKAAIQDDTRAVYLETLGNPN
                     ADIPDIDAIAELAHAAGLPVVVDNTFGTPYLFRPFEHGADIVVHSATKFIGGHGTTLG
                     GIIVESGRFDWKTSGKYGNIADPNPSYHGVSFYDAAGPAAFVTYIRAILLRDTGAAIS
                     PFNAFLLLQGTETLSLRLDRHAENTRKVVEFLRNHPKVARVNHPSLEEHPDHELYEKY
                     FPNGGASIFTFEIRGGKEEAWKFIDNLEIFSLLANVADVKSLVIHPASTTHSQLTDEE
                     LADQGIKQSTIRLSIGTEHIDDIIADLEKGFAAI"
     gene            complement(4257..4901)
                     /gene="pcp"
                     /locus_tag="ACZPCG_01205"
     CDS             complement(4257..4901)
                     /gene="pcp"
                     /locus_tag="ACZPCG_01205"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015561367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HIR1283722.1"
                     /translation="MKLLLTAFDPFGGEPVNPALEAVKKVQNQIGSVSIVKLEVPTVF
                     GTSIQTVAKAIEQEMPDAVLCIGQAGGRYGLTPERVAINLDDARIKDNEGNQPIDLPI
                     FKDGAPAYFSTLPIKAMVQSIRQAGLPASVSNTAGTFVCNHLMYGVLYTLERKYPGVR
                     GGFMHVPFIHSQTVNRPAPAPSMSIEDIAAEIEAAIRAIGEHESDIKAAEGATH"
     gene            complement(4949..5878)
                     /locus_tag="ACZPCG_01210"
     CDS             complement(4949..5878)
                     /locus_tag="ACZPCG_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009220318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HIR1283723.1"
                     /translation="MTWTLSTAVAEFFYCIIGLIFIGTGFKALKDPTCEKRITTAAFW
                     FILAATFIAGPYFPRWITGLCVVLMAILTAFKRVAPSSSDVPAAAKTRAAADRLGYSV
                     FIPPLLLAFSAVLVATFFKSLGANNAIGVSGVIALIAAFLLFRPKPVYAAKDCTRLTD
                     NVGVTGILPQVLAALGAVFTAAGVGDVIAAGVSAVIPSGNLFAATAVYCIAMAVFTMI
                     MGNGFAAFSVITVGIGIPFLIAQGADPAVVGALGLTAGYCGTLMTPMAANFNILPAAV
                     LETKNKYTVILSQLPMALFMLAIHIVLMYFLAF"
     gene            complement(5880..6575)
                     /locus_tag="ACZPCG_01215"
     CDS             complement(5880..6575)
                     /locus_tag="ACZPCG_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008755316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HIR1283724.1"
                     /translation="MEIVKLIGVLIVIAGFILKLDTLAMVVTAGLATGLVAGMSPLEI
                     LDTLGTAFITNRTATLFILTLPVIGLCERNGLKDKAVDFIKSLKNVTTGRLIAIWQAI
                     RTVASAFSLRIGGHVQFIRPLINPMAQAAAVVQYDKLDEKTEDQIKGMCSGSENYGNF
                     FAQNCFMGSSGTLLIVSTLSEQGYPVDALQIAGQSIPIAVLSLVAGVVYALLFDRILK
                     RRFGDKRRKKEAN"
     gene            6910..9858
                     /locus_tag="ACZPCG_01220"
     CDS             6910..9858
                     /locus_tag="ACZPCG_01220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1283725.1"
                     /translation="MPYFDLHFSKDALGLLCDEDRMEVEHAIMSAKSRHIGIKVYSPV
                     KEKTSCLKWYKIEGWEDGTTYCLLFGGMSQETQLFQSIASENADNIYVIDKENYDLLY
                     VNGFKRAFWNEEGQNTPKCYEHIYNKKEPCFHCMMKMGGSEEMPCETSYEEGGRCYAT
                     HFKETEWNGTPAYIKYVRDITEEATVKREKERLEKYFETVLKYLPGGVAVVHHGLNGR
                     LTPEYLSDGFAEMVDMSMEEAWDMYKTNALSGVHPDDQEYVKQTLDRCIRENCDREAL
                     QYRLKKGSGGYIWVNTKFSVIQCEAGDAMVYADYHDITEEREMQERLRLQYREQIFQH
                     YLITDPNTLILGHCNITKNKIIEIEDRTNSGLLERFGDKREDFFTGIGTLVASEAERS
                     LFYEKYLNEPSIRAFENGIKEVLMPCFVKLPNYPQGRYVQFKVNLVETPDTRDITGIL
                     TVTDITEKTIQDRIIRMLSSLNYDLVADVDLINDQYKVVSGGDHNITETQGSLMERVR
                     RVVEELVVDSEKAYVRDMLDPGKMVSRLKDGEFYSFRYSVNNGSNEIQTKNMIISAID
                     LSLGRICLVRTDVTDMLAAERKVKYDLEKALSEAEKASRVKSDFLSYMSHDIRTPMNA
                     IVGMTTLAQANLSDSRKVEDYLKKISVSSQHLLSLINDILDMSQIEQSKLHMNVQVIH
                     IDELLDQISSIMASPSKDAGLKFMVERGEIQHLYFQGDALRIKQILINILSNAFKFTM
                     EGGNVRFRTEEIPSYESGRVRYRFVISDTGIGMTQEFICHLFEPFCRSERVNRVEGTG
                     LGLSITKGLVDLMGGKIRVESQLHQGTMFEIELEFAVADGKQKRSPNADECIPEESLE
                     GRHFLLVEDNAINSEILAELLQMWGATCVLRENGLQAVEEFESSVPGTYDAILMDIQM
                     PVMNGYEATRKIRSSSHPEAKTMVILAMTANAFAEDVQKSISAGMNGHVAKPVDMKIL
                     YNTLMALLDD"
     gene            9881..11131
                     /locus_tag="ACZPCG_01225"
                     /pseudo
     CDS             9881..11131
                     /locus_tag="ACZPCG_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587724.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease Q family protein"
     gene            11173..11241
                     /locus_tag="ACZPCG_01230"
     CDS             11173..11241
                     /locus_tag="ACZPCG_01230"
                     /inference="COORDINATES: protein motif:HMM:NF012789.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1283726.1"
                     /translation="MREWNRDGKELFVIGDPADEPV"
     gene            11358..13028
                     /locus_tag="ACZPCG_01235"
     CDS             11358..13028
                     /locus_tag="ACZPCG_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1283727.1"
                     /translation="MEKYRKIAGKCLAAVLSAAMALSLAACAGGTGSSESQSQSAKQE
                     AAAEKPAQATGEKIVNVGVTGTVNTLNPLLMDGVEINKYATGLMFLPLVELDTNMEFE
                     PMLADSITAEDEKHFLVHIDDEAVWSDGQPVTADDVVYTALRLCSPAIANTAMMYYVF
                     EGVGDDGFVEMGADHIEGITKVDDKTVRFATKEPMSLVTFQNSYARYLMPLPKHVIGE
                     IPEAELASHEWFAKPDVVSGPYRVTAFDKDHYVSYEANEKYWKGAPKIPKLNIRIVEG
                     SQLYAGLQSGEIDITQNTMSAIPLEDYESLQALETVETSFGDAITNQSVFINTKNVPD
                     QKFRQALLYAIDRNQILEQLLKGNGEVVDGFLSTASPYYDPSIEPVSYDPAKAKELLA
                     ESNWMPSSPLRFYIDSGDSTFVNAASVIAAQWAAVGIQTEIRTMDINTLMSTAGSGDF
                     DIMAVQYTYPPVDPYVDIAWLLGGEGSWTGYSHAEAEEALLKIAVLSDKEELKEQYGI
                     IDRRMQEDVPMFSAYVIRTMAAKNKRLTGVNPSVYGFFNHVEEWDITG"
     gene            13136..14149
                     /locus_tag="ACZPCG_01240"
     CDS             13136..14149
                     /locus_tag="ACZPCG_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1283728.1"
                     /translation="MSHILEVRGLQVAFAGERGNTIGTDRIDFFVDQGETVCLVGESG
                     CGKSITSLAVMGLLGRGGRVSQGEILFNGRNLLKMDRKELDQVRGKELSMIFQDPLSS
                     LNPVLTIGKQLEEGLKAHTELGKEERRKRALDLLGRAGIREAEAVMKKYPHQLSGGMR
                     QRVMIAMALSCQPSLLIADEPTTALDVTIQAQIMNLLRSLKRELRMSLLLITHDMGLV
                     AQMADRVMVMYAGQIIEEGTVFEIFDHPSHPYTKALLAAVPSMEQNPEKMLQAIEGTV
                     PENYGQMTGCRFRDRCPYRKQGCEAPQELEEIKAEETQKAQELHRVRCWRAKEAEQSG
                     RTL"
     gene            14133..15161
                     /locus_tag="ACZPCG_01245"
     CDS             14133..15161
                     /locus_tag="ACZPCG_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007718836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1283729.1"
                     /translation="MAEPYKNTEPLLKVEHLKKYYPQNRPIFGRPTSWIRAADDVSLE
                     LYPGETLGLVGESGCGKSTLGRMIAGLEAPDSGCIWYKGQELTAKGGRGYSAVRTEIQ
                     MVFQDSYSSLNPRKQIFQILSEPMLYHSLADKHTVHKKAEHLMELVGLPLSALDRYPY
                     EFSGGQRQRIGIAKALSLEPKLLICDEPVSALDVSIQAQILNLLKSLQKELDLTCIFI
                     GHGLGAVNYVSRRIAVMYLGKLVETGESSELFEHPVHPYTRTLLGAVPIPDPRKREEN
                     WGETSEGTAYEPSITGAEDKGCPFKDRCFRKRPECSSAEVPLRPVRPGSTHLAACSPA
                     VLEVKRNE"
     gene            15158..16105
                     /locus_tag="ACZPCG_01250"
     CDS             15158..16105
                     /locus_tag="ACZPCG_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1283730.1"
                     /translation="MKKYIIKRLLIAIPTLIGITLIDYAIMCFAGSPLEMLQGARISQ
                     EAVAAKEAALGLDKPFYVQYFIWLGQVLKGNLGVSIKTYQPVSQMIMERLGPTLLLMG
                     VSLAVSLLISIPAGIYSAVRQYSKGDYAVVTASFLSSSIPGFFLSLLLVYIFNIKLKL
                     LPSSGMITLGGSGGIGDLAAHMVMPVTVLALSMAGTNIRYIRSSMLEILQKDYLRTAR
                     AKGIGERLVIGRHALRNGLVPVVTVIGMQIPLLFGGAVIVEQLFSWPGLGLMTMSAIL
                     SRDYPVIMGVCLLAAVVVLAANLVTDILYAVVDPSIQYD"
     gene            16120..16992
                     /locus_tag="ACZPCG_01255"
     CDS             16120..16992
                     /locus_tag="ACZPCG_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1283731.1"
                     /translation="MKKQMKNHETYAGAVWRRFCRHRLALASLAVLVLICGSAVFAPV
                     IAPYDPEAIVGPFGAPPGDEFLLGTDQIGRDMLSRLLYAARTSLFAGVLATLIATALG
                     VFLGLLAGYFGGLADILIMRFTDMVMSFPYILLVLVAAAIFKPGLWSIILILGFVDWP
                     GIARLVRGNVLSLRETNFVKSSEAAGMPAFHILFSEILPNTMAPVLVYATSVMALTML
                     DEAALSFLGMGVQPPAASLGNMLNSAQSVTVLTKQPWLWIPPGLVIIIVVTAVNFVGD
                     ALRDAFDPLSIIKM"
     gene            17346..17609
                     /locus_tag="ACZPCG_01260"
     CDS             17346..17609
                     /locus_tag="ACZPCG_01260"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HIR1283732.1"
                     /translation="MKGWAHTMSSLSMFSQLGLSLAAPLLLCVLICSWLTGRFGIGTW
                     IYIPGFFFGLGGSGATAWKVYQNIMKHQKKKKRTNERAFNEHF"
     gene            17622..18044
                     /locus_tag="ACZPCG_01265"
     CDS             17622..18044
                     /locus_tag="ACZPCG_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1283733.1"
                     /translation="MAQIQPAVKKETARVTGITLTGCVIMFAAFFVLHKISPASVPFD
                     MKVIISGLLGSGVAVANFFFMGLTIQKIVSGEDEEQARKDMKASYRFRTVTQLLWVVL
                     AVALPFLNGAAGLIPLFFPSAGIKVLSMFGMINAKPNP"
     gene            18066..18827
                     /locus_tag="ACZPCG_01270"
     CDS             18066..18827
                     /locus_tag="ACZPCG_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009216294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HIR1283734.1"
                     /translation="MNFDISGAAIYLRIPTGIPVLGDFVITETMVVSWLVMILITGFC
                     IWLTRDLRVENISKRQAVAEMLVEQATNLVRNNTGGTKFDYLIPFVAALFTTSAVSNL
                     ISLTGLRSPTADLSVEAAWAVVVFVMITANKIKASGLLGYMKGFTTPIPVMTPFNILS
                     EIATPISMACRHFGNILSGVVINGLLYAALAVASSALLGWLPGALGEILGSIPILDVG
                     IPAILSVYFDWFSGLMQAFIFSMLTIMYIANAAEG"
     gene            18876..19157
                     /gene="atpE"
                     /locus_tag="ACZPCG_01275"
     CDS             18876..19157
                     /gene="atpE"
                     /locus_tag="ACZPCG_01275"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009428481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HIR1283735.1"
                     /translation="MTDFQVLAKGIALAGCAIGAGCALIAGIGPGIGEGNAVAKALEA
                     IGRQPECKSDVTSTMLLGCAIAETTGIYGFVTGLLLIFVAPGTFMNFLS"
     gene            19200..19706
                     /gene="atpF"
                     /locus_tag="ACZPCG_01280"
     CDS             19200..19706
                     /gene="atpF"
                     /locus_tag="ACZPCG_01280"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HIR1283736.1"
                     /translation="MRETQELVTIVPWTFIATICNLFIQCWLIKKFLFKPIREVIEKR
                     RALADSQIQDAKKAKEEAETMKAGYEAEMAKAKETASVILQDAQKDAAARSEAMLQEA
                     KAQAAGIKAKAEADILQEKKKAVNEIKNEIGGIAMDIAGKVIEREISEDDHRKLIDEF
                     IENVGEAS"
     gene            19703..20242
                     /gene="atpH"
                     /locus_tag="ACZPCG_01285"
     CDS             19703..20242
                     /gene="atpH"
                     /locus_tag="ACZPCG_01285"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005428175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HIR1283737.1"
                     /translation="MTKTARLYGGSLYDLASEEKLTEEMLEQLDMVRALFKEEPSYLR
                     LLAEPSIPKTERVGLIHTAFEGKIHVYLLNFLKLLCENGMLREFSDCCREFRTRYNED
                     HGIAEAVVTSAVPLTEEQKEALKARLEKISNKTIQLTLKVDPQVVGGIRAELEGRQLD
                     GTLQERLSGLRKKVTEIIV"
     gene            20257..21777
                     /gene="atpA"
                     /locus_tag="ACZPCG_01290"
     CDS             20257..21777
                     /gene="atpA"
                     /locus_tag="ACZPCG_01290"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013279575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HIR1283738.1"
                     /translation="MQLKPEEISKVIRSQIKYYQNAIEQSETGTVLMVGDGIARVSGL
                     TNCMSGELLEFEDGTFGMAQNLEETNVSVVLFGSDQGISEGQTVKRTGKVVSVPVGEA
                     MIGRVVNAIGQPIDGAGPIETSEYRPVESPAPGICDRQPVKEPLQTGIKAIDSMIPIG
                     RGQRELIIGDRQTGKTTIATDTILNQKGKDVICIYVAIGQKRSTVANLVSELQAGGAM
                     DYTIVVTATASETSPLQYIAPYSGCAMGEYFMNKGKHVLIIYDDLSKHAVAYRALSLL
                     IRRPPGREAYPGDVFYLHSRLLERAAKLSDEKGGGSLTALPIIETQAGDVSAYIPTNV
                     ISITDGQIFLETELFHSGIMPAVNPGISVSRVGGNAQIKAMKKVAGTLKLVYSQYREL
                     QSFAQFGSDLDADTKARLAQGARIVEVLKQGRSTPVPVEKQVAILYAVVNGILTQVEV
                     EDIQSYEEGLYTFLDSDAAGVSAMEGIRSTGKLEGETEEKLKTALKDYTDRFLKAR"
     gene            21799..22656
                     /gene="atpG"
                     /locus_tag="ACZPCG_01295"
     CDS             21799..22656
                     /gene="atpG"
                     /locus_tag="ACZPCG_01295"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009429496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HIR1283739.1"
                     /translation="MAANMKAVKLRMKSIQSTMQITKAMELVASSKLRRAKERADASR
                     PYFHTLRDTLADIAAGNTDFSSVYTRSGKSGRWCYVVVAGDRGLAGGYNANLFKEMEA
                     QMAGKDCIVLPIGKKAVEYYKRRKVEILTEEFAEVGDLSVSDCFTIAKLLCKGFKSGD
                     FGHAALGYTNFISMLSQRPGVSGMLPLEDFSGEEKDVKKIRSLILYEPDAETVFDSIV
                     PEYLAGLLYGAMCESVASELAARRTAMDAATKNAGEMIEQLSLYYNRARQASITQEIT
                     EIVAGAEEP"
     gene            22755..24152
                     /gene="atpD"
                     /locus_tag="ACZPCG_01300"
     CDS             22755..24152
                     /gene="atpD"
                     /locus_tag="ACZPCG_01300"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007157829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HIR1283740.1"
                     /translation="MTEKHIGKVVQVTGPVLDIRFREGELPALLNAIEIDVNGHRLVA
                     EVAQQVGDDVVRCIAMSSTDGLVRGADAVDTGSAITVPVGDECLGRVFNLLGDPVDEK
                     PAPEDVERWAIHRPAPSYEEQMPATEILETGIKVVDLICPYAKGGKIGLFGGAGVGKT
                     VLIMELIHNVATAHGGLSVFTGVGERTREGNDLYGEMKESGVIDKTALVYGQMNEPPG
                     ARMRVALSGLTMAEYFRDVKNQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQPTL
                     ATEMGALQERITSTKKGSITSVQAVYVPADDLTDPAPATTFTHLDATTVLSRDIASQG
                     IYPAVDPLESTSRILSPEVVGEEHYQVARSVQRVLQRYKELQDIIAIMGMDELSEDDK
                     LTVSRARKVQRFLSQSFSVAEQFTGMAGQYVPLKETIRGFKKILDGECDEIPESCFLF
                     AGTIDDVFEKAKKAQ"
     gene            24171..24575
                     /gene="atpC"
                     /locus_tag="ACZPCG_01305"
     CDS             24171..24575
                     /gene="atpC"
                     /locus_tag="ACZPCG_01305"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009536610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HIR1283741.1"
                     /translation="MAAFHLQIVTMDGNVFDGQVQSVSCRTIHGDLAIMARHMNYCTA
                     VGMGTAHVIMEDGTERTAACIGGMLTVMNGECRLIPTTWEWSDEIDLERAKRAKARAE
                     EKMNKSGLSEVERQMIEAKLHRALVRIGTVEK"
     gene            24723..25814
                     /locus_tag="ACZPCG_01310"
     CDS             24723..25814
                     /locus_tag="ACZPCG_01310"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIR1283742.1"
                     /translation="METKRIYIKDTEHVRDEELLEAAEIIRRGGLVAFPTETVYGLGG
                     NALDEDAAKKIYAAKGRPSDNPLIAHVSCPEEVEPLVEYMPEAGKKLMEAFWPGPLTM
                     IFPKSSRVPYGTTGGLETVAVRMPSDPVANRLIALAGVPIAAPSANTSGRPSPTTADH
                     VWQDMNGRIDMIIDGGPVGIGVESTIVDVSGQVPVVLRPGAITMEMLRETLGEVTIDP
                     AILGPMAEGVRPKAPGMKYKHYAPKAELTLVEPAMEDKKASMTQEQVERMVSMVWKLA
                     KEQIDAGVRVGIICTDESRAAYPEGIVRSIGARKSQESVAHNLYGVLREFDDLKAEVI
                     FSESFGEDHLGQAIMNRLSKAAGYKIVRV"
     gene            25829..26506
                     /locus_tag="ACZPCG_01315"
     CDS             25829..26506
                     /locus_tag="ACZPCG_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HIR1283743.1"
                     /translation="MEKQTLGEKTAQMLLEMIQKEGFGPGDKLPTEAELVESLGVGRN
                     TVREALRILMSRNIVTIRQGSGTFISEKKGVVDDPLGFSMMEDRRRLTEDLIQVRLML
                     EPQLAALAAQNAAEEDLQKLEQIKEELEDLIRQRKDYSLKDSQFHEQIANCSHNQIMT
                     NLVPVITEGVRMFAVSVQETEYEQTLSSHRRIFEAIRERRGEDAKMEMYFHLMYNSNR
                     YRAETEP"
     gene            complement(26569..30492)
                     /locus_tag="ACZPCG_01320"
     CDS             complement(26569..30492)
                     /locus_tag="ACZPCG_01320"
                     /inference="COORDINATES: protein motif:HMM:NF012516.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HIR1283744.1"
                     /translation="MQTFASSQNSLTASHSDANRDDFTPSPIYQPLATPSDAEHSSSE
                     DRDEDNEIIATPSDATSSDAHKRPASPSDAVSTPSNAAPKIERRKTPPLTEPKYDRTE
                     GSEDVTNLLVPTLAYDDTSVTLVWNKPEKYDTVADYAVYQDGELIGTARENFAEHADW
                     ASTYMDAFYEYYEKKNIEMVNVDIHSFTADNLTPDTSYEFSVVALDDSGNPIGDTASV
                     SASTAPAPEIFNITDFGARTVDTPYRSYDDGINRFIEENTKAIQAAIDACTEGGKVVI
                     PSGIFMSGALYLKSNMTLELEKGAVLFGSPNADHYDSNYLLYPYSTDTRSWALINAYS
                     SDEGGMLENIRITGEGTIDGNGWKYGEKDDINGDGYSMFYQDRQAADPEDKAYRLPRW
                     VSGNSKKLYTTPFETSLGILASDATLKAKEKGLSEKVAYNNRPNLIVIRGTENVYIGD
                     ITVRNPAFHTVAVLDSKNVTSNNVKYTTYDSNNADGIELGNTQNAIVFNNFFDTGDDS
                     INFATGMGKGVQDCEQKPSQNIWTFNNFLREGHGGAIAAGSHTGAGICNMLVEDNVLN
                     HSDMPFRFKSAPANGGGVWDVLIRDCAVGECSQAFVMSTTYSDVNQAVSVEPADTPAE
                     FRNINAYNLTIDDVKKNEFSLVADVDYSQLEKPWHTHHDLYFQDITFTNSKMAQELKG
                     VEDSVFYNVRFNDKDEDSKAWSKISASGGLNFAGDTTTSDKAENAMDIPEWTEDAVLT
                     ASPSNASRASADEAAELTWTDAADYDEISGYIIETRADGQLVDISTPVTKPEYQAEGL
                     CANVDYTFTVYAVDATGNKTEGPSAGLRIESAQDETLNVPSDRKVRYSGEGYTWASAT
                     FANGSKADPRIRGYHAYADGVLVKTIYNYELGSKSSQDELTLTIGRLMETENEVIITA
                     FADNGDTFDYESETVKLSHKYDFKAPEWDSSLDVREENGDLILSWEEPWDESGIYGYR
                     IYMDGKPVYADEDDYFNHVNSSCTTTKTSYRISGADLETSHTFKVEAADNWWKALNGT
                     GPFHWTYSGPQAVWNREEVTVSLSPQNARIAVGGSIQLHAEITPAGLTASWTSSDPST
                     ASVDENGLVTGLRPGQAVITVSAGGQSASSAVTVNDENVPEIPSIPDNSDSDGSSGSG
                     TSVSGPGSSVSSHTILDSKKGYVNTVSGIITGNGTNNSHWIQESGNPSQWKLQYADGT
                     FAAGSIKTAENGTVYEQPAWEMINGAWYAFGADGYTKSGFFFDHEQNSWFFIDINSGM
                     KTGWQQIENNWYYFNPVSDGTMGKLYTSATTPDGYQVDENGRWIQ"
     gene            30922..32379
                     /locus_tag="ACZPCG_01325"
     CDS             30922..32379
                     /locus_tag="ACZPCG_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1283745.1"
                     /translation="MKELYDVLVIGGGVVGSAVARELSRYRLRIGVLEKNLDVCNETS
                     GRNSGVVHGGFAYDRGSLKAKLCVEGNQMMGELAKELDFRFIRCGKVLVGNTDEDMET
                     LKRTIRQGEDNGASGLELIDEKRLHELVPAVVGKFAMYSKNSGIVDPFGYTIALAENA
                     HANGAEYHFGCEVTDISRDGDGNYCISTSGGTFYSRWVVNSAGLGCGKISDLLGITGY
                     HIIGSKGDYIILDKRTGPLLPMPVYPVPSNTYMGIHVTNTTDGNVIIGPNADLVTDFT
                     YYGVPQENMDYLANSASDLWPCIRKQDYIRNYSGILPKWVDDQGVIQDFKIEIRDDLA
                     PRAINLVGIESPGLTAAVPIARYAIEMMKEREKLEENPDFNPERKGIRRFEECSMEEQ
                     ARLIEEDPDYGEVICRCEKVTKAEILKAIHNPLGVDTMTGIKYRTRSMMGRCQGGYCQ
                     MRIEQLIEQELGKKETQVQYEREGSQVLFGKVREV"
     gene            32383..33645
                     /locus_tag="ACZPCG_01330"
     CDS             32383..33645
                     /locus_tag="ACZPCG_01330"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007718785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR1283746.1"
                     /translation="MGQLEHEIVCEAVDAVIVGGGPAGLAAAVRLYEKGITNILILER
                     EKHLGGILRQCIHDGFGLTRFGLTLSGPEYAQKFIDRVHELKIPYLTDATVLEVTKEH
                     VVTAATPQGLKQWKAKAVILTMGCRERTRGALGIPGERPAGVFTAGVAQAYINLYNRM
                     PAKEVVILGSGDIGMIMARRLTLEGAHVKAVFEIQPYPSGLPRNIEQCLNDYGIPLYL
                     SHTVTAIHGDKRLTGVTVSQVDEHMKPIPGTEKEYDCDTLILSVGLIPENELSLDAGV
                     TLDGRTKGAEVDEYFQTDRPGIFAAGNVLHVHDLVDFVSMEAERLADAAAEYVDKGCL
                     PECPVVIRTDCFINHTVPQRISGKEDCRLSLRVNRPFKDCRIVVRQDGTEVAFKKMKK
                     AIPAEMIQIPLKAEKLSGCKELEVSVEW"
     gene            33639..34019
                     /locus_tag="ACZPCG_01335"
     CDS             33639..34019
                     /locus_tag="ACZPCG_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HIR1283747.1"
                     /translation="MVREFTCIICPNGCEITAELEKTEDGACRIRSIEGAACPRGKAY
                     VEQELTDPRRNIATSISVEGGELPLTSVRLTAPIPKARIFDAMEEIRRCRVTAPVKAG
                     TVVIEGLLGYDSDVIVTKTVEKQK"
     gene            34134..34421
                     /locus_tag="ACZPCG_01340"
     CDS             34134..34421
                     /locus_tag="ACZPCG_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1283748.1"
                     /translation="MKERLQKEEKCMSLFIGKTERVYSTSDSFQKERVQEILNENHIE
                     YKIRARDDSRRNPFDQAGLGSLGNNRLLVTYSFYVDKKDAEWEVRLIHSQV"
     gene            34516..35010
                     /locus_tag="ACZPCG_01345"
     CDS             34516..35010
                     /locus_tag="ACZPCG_01345"
                     /EC_number="3.5.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004132 - dCMP deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006220 - pyrimidine nucleotide metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009972 - obsolete cytidine deamination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxycytidylate deaminase"
                     /protein_id="HIR1283749.1"
                     /translation="MSGKREDYITWDEYFMGVACLSGKRSKDPSTQVGACIVSQDNKI
                     LSMGYNGFPKGCSDDEFPWEKEHEADDPYHAKYFYSTHSELNAILNYRGGSLEGSKLY
                     VTLFPCNECAKAIIQAGITTIVYGEDKYADTPAVRASKRMLNAAGVRYYQYQPTGRKI
                     EIEV"
     gene            35016..>36860
                     /locus_tag="ACZPCG_01350"
     CDS             35016..>36860
                     /locus_tag="ACZPCG_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HIR1283750.1"
                     /translation="MKVLLAAVNAKYIHSNLGIYSLKAYGEKMLAEQGFKREQIQIEL
                     AEYTINHQLEQILKDLYCRKPDVIGFSCYIWNISYIKCLLADIKKVLPEVKIWLGGPE
                     VSYCGEEILREQPVVDLVMKGEGEETFAQLLQVLVEKEGSGASFSGYLEEIKQKGIPG
                     LVFLDSQGNKDGAAWGTFAGGDRQAGWPVYDTGIRPPMDMNRIPFPYGFLEMEQLEHR
                     IIYYESSRGCPFSCAYCLSSIDKSVRFRSLERVKEELSFFLNARVPQVKFVDRTFNCK
                     KSHAMAVWQFIRENDNGITNFHFEIAADLLDQEELNLLSSMRPGLIQLEIGVQSTNPD
                     TLSAIRRKTDIAEIRKITHHINSRHNIHQHLDLIAGLPHEDLSSFRKSFNEVYDMEPE
                     QLQLGFLKVLKGSYMEEMIPACELLYSSSPPYEVLSTRWLSYGDILELKAVEEMVEVY
                     YNSRQFTCTLEQLRQEFDTPYEMFLDLAGFYQKKGYGGVNHSRLARYEILWEQAEEMF
                     SDGSRTDAERKREQYRDALMTDLYLRENIKSRPSFARNLSHFKEQIRRFFRQEEDCPC
                     LLPDYTGYDSRQLAKMAHLEPAGDGSFLLFDYKNRDPLTYNARMVRVEI"
BASE COUNT         9976 a         7761 c         9951 g         9162 t
ORIGIN      
        1 aggaaggcag agcctttaac tttcattatc aaagcaatgg ataccatcca cttgtctgtt
       61 atgatggaat gaccggagat ctggtaaaaa tccagcttcg tgattgattt atacaccatc
      121 agtcagaaag aactgttaaa cattcaataa ggagtgcatg gatattttta aatggtatag
      181 atgaattcag aaacctgacg gttgataata tcttctaagg taatactgtc gaaatatttt
      241 gtgacaacat cgtacaggcc ttcataaatt ggtaaagtaa tacagtcttc acatttttta
      301 cacaggtttg gggtgttgtc catacagctt acaggaacaa gacttccctc agcgctttct
      361 aagatttcac ggactgtgat ttcggaaggc gcttttgcca gcctgtaacc gcccatatgt
      421 ccctttgttg cgctaagtaa attacctctg ttcaggaacg ggatgatctg ctccagatat
      481 ttctttgaaa tatcctgtct ttttgaaatg tcttttaatg cgataaatcc ctgatcataa
      541 tgctgcgcca gatctaccat catgcgcagg gcatagcgtc ctctggttga tattttcata
      601 ggatcacctc ctcgctgcct attatactac agaaaagata ggctttcaac gcttaaaaat
      661 agattgacaa atggaatctg tggcactata cttacataaa catatataac ctatataaat
      721 aataggcaat aaaagaatgg aggaaaaaat catggcagat atcaggaaaa gctcattaga
      781 attaattgga ggaacaccgc ttctggaggc gggaagatac agtaaaaaag caggggtaaa
      841 taaggcggtt attttggcaa agctggagta tttgaacccg gcaggatctg tcaaagacag
      901 aattgcgctt gctatgatcg aagatgcaga gaaacgcggc attttaaagc ctggagcgac
      961 gattattgaa ccgaccagcg ggaatactgg aatcgggctt gcggctgtgg ctgcggcaaa
     1021 aggatatagg gctattctta cactgccaga taccatgagc attgagcgcc ggaatctcct
     1081 gaaggcttat ggagccgagc tggtactgac ggaaggttcc aaggggatga agggagccat
     1141 tgcgaaagca gaggaattaa acagggaaat tgagggctcg gttattctgg ggcagttcat
     1201 taatcccgca aatccaaaga tacataaaga gacgacaggg ccggagatct ggaaacagac
     1261 agatggaaag gtggatatct tcgttgccgg cgtcggaaca ggaggaacca ttactggtgt
     1321 gggagaatac ttaaaggaac agaatccgga tatccagatt gtagcagtgg aaccggcagg
     1381 aagtccggtt ctttccaagg gaaccccggg agcccataag attcagggga ttggagccgg
     1441 atttgtgccg cagacactta atacggagat ttatgatgaa gtcattacga ttgagaatga
     1501 agacgcattt aaagaaggga gagcttttgc aacatcagaa ggcatcctgg tcggcatttc
     1561 ttccggagca gcattaaagg ctgcggttat ccttgcaggc agacaagaga atgcaggtaa
     1621 agtgatcgtt gcattgcttc ccgactctgg agaccggtat ttgtcaacag ccttgttcag
     1681 cacaaattaa aactgcaggt gatacaatga cattaacaca gttacaatat gtagtggcaa
     1741 ttgcggaaac aaagtccatg aataaagcgg cggccgcttt atttgtatca cagccggcat
     1801 tgtcaggtgc aattaaggat ttagaagagg agcttcaaac ggaactgttt atcagaacca
     1861 ataagggaat cgtgatcaca acagaaggcc aggaattttt aagctacgca aggcagatgg
     1921 ttgagcttgg aaatctggtg gaagagcggt ttgtgaataa aacaatagca aaaaagaagt
     1981 ttagtgtgtc catgcnnnnn nnnnngatat acggacagta tgaaagtgtg gttgagaaat
     2041 acagtgaggg tggttcctat aagaagaaat ttggcgtttc cacccagcat tattcttttg
     2101 cagtggaggc atttattaaa ctggcaaaaa atttttctat tggggagtat gaactggcgg
     2161 ttcatgaaac aaagacccac gaagtgattg ataatgtaaa ggattataga agtgaacttg
     2221 gtgtgcttta cttaaattcc tttaatgaaa aagcaatgac cagaatactg gaggataatg
     2281 atctggaatt catgccttta tatgactgtg gcatatctgt ttaccttagt aagaaccacc
     2341 ccctcgcaga gaagacgaag attacaatgg acgagctgtc cgagtatcca tgcctgtctt
     2401 ttgagcaggg ggaaaggaat tccttttact ttgcagaaga ggtattcagt acccatgcat
     2461 acaaacagat catcaaagca gatgacagag cgacgatgct gaatcttatg acaggattaa
     2521 atggttatac tctgtgcagc gggatcatat gtcaggatct gaatggggat ttctacgtgt
     2581 cggttcctct tgatacaaca gataccatga caatcggtta tatcaagcgg aaaaatatac
     2641 ccctcagcct tctgggcaga aagtatattg atattcttaa atcctataaa tgagttcggt
     2701 atttcagcgg tttattcatt gttataatgg aaacttatag ccagttctaa cttctatgta
     2761 ttttacagga aatgagaagc cggttataat aggcccataa aacctagtta acatatagga
     2821 aacagaggcg aatcggagga gaatgatatg agcaaataca gatttgagac gctgcagtta
     2881 cacgtaggac aggaacatcc agatccggca actgattcaa gagcggtacc gatttatcag
     2941 acaacatctt atgttttccg aaactctcag catgcggcag accgatttgg ccttgcagat
     3001 gccggtaaca tctatggaag acttacgaat tccacacagg atgtgtttga gaagagaata
     3061 gcggcgcttg agggcggctc cgctgccctg gcattagcaa gtggagcggc ggctattgct
     3121 tataccattg aggctctggc ggcaaacggt gggcatattg tcgcacagaa gacgatttat
     3181 gggggaagct ataatctgct tgcccatacc cttacccagt atggaattac cacaactttt
     3241 gtggatgccc ataatcttga ggaagtaaag gcagcgatcc aggatgatac cagggctgtc
     3301 tatcttgaaa ctttaggaaa tccgaatgca gatattccgg atattgacgc cattgcagaa
     3361 cttgcacatg cagcaggact tcctgtcgta gtagataata catttggcac accttatctc
     3421 ttcaggccct ttgagcatgg cgccgatatc gtcgttcatt cggcaactaa gtttattggc
     3481 ggacacggaa ctacacttgg tggaattatt gttgagtccg gaaggttcga ctggaagaca
     3541 agcggaaagt acggcaatat cgctgatcca aatccaagct accatggagt aagcttctat
     3601 gatgcggcag gccccgcagc atttgtaact tacatccggg ccattcttct ccgtgatacg
     3661 ggggcagcca tttcaccctt taacgcattt cttttgcttc aggggacaga gactttatcc
     3721 cttcgtctgg acagacatgc agagaacacc agaaaggttg tagagttcct aaggaaccat
     3781 ccaaaggttg cccgggtgaa tcatccgtct ctggaggaac atccggatca tgagctctat
     3841 gaaaagtatt tccccaatgg cggagcgagc atctttacgt ttgagatcag gggtggaaaa
     3901 gaagaggcat ggaagtttat tgataacctt gagattttct cactccttgc aaatgtagcg
     3961 gatgtgaaaa gccttgtcat tcatccggcc agcacaaccc atagtcagct tacagatgaa
     4021 gaacttgcag atcagggcat caaacagagt acgatcagac tctcgattgg aacagaacat
     4081 attgacgata ttatcgctga tcttgaaaag ggatttgccg cgatataaag gaatgaaggc
     4141 agctggctca attacccagg gaatttcctt attaaaacag gaaattccct ttttgatgtc
     4201 atcaacgatc cgtgaaatct ttcgtaagca gccagtttga tttttataaa ataagatcag
     4261 tgtgtagcgc cctcggcagc ctttatatca gattcatgtt cgccgatagc tctgattgcg
     4321 gcttcgatct cggcagcaat atcttcgatg ctcatggaag gggcgggagc cggacggttg
     4381 acggtctggc tgtggataaa gggaacgtgc ataaaaccgc ctcttactcc ggggtatttc
     4441 cgttccagag tataaaggac gccatacatc agatgattgc atacaaaggt tccggcagta
     4501 ttcgatacgc tggcgggaag tcctgcctga cggatagact gcaccatagc tttgatgggc
     4561 agggtagaga agtaagccgg ggctccgtcc ttaaagatgg ggagatcgat gggctgatta
     4621 ccttcattat ctttaatacg ggcgtcatcc agattgatcg ccacccgttc gggcgtcagt
     4681 ccatagcgtc cgccggcctg tccgatacac aggacggcgt ctggcatttc ctgttctatg
     4741 gcctttgcca ctgtttgaat ggatgttccg aatacagtgg gaacctccag cttaacgata
     4801 gaaacagagc cgatctggtt ctgtactttt tttacggctt ccagagccgg gttaaccggt
     4861 tcgcctccaa agggatcaaa tgctgtaagt aaaagcttca taataaaata tcctttccgc
     4921 agatggtgtg aaaacagctt caaatgtatc agaatgccag aaaatacatc agtacaatat
     4981 ggatggccag catgaaaaga gccatgggga gctgggataa gattacggtg tatttgtttt
     5041 tcgtttccag tactgccgct gggagaatgt taaaattagc agccatgggc gtcatcagag
     5101 taccgcagta gccagctgtc agtccaaggg cgcctacaac tgccggatcg gctccctgtg
     5161 cgatcaggaa aggtataccg atcccgacgg taattacgga aaaggcagca aaaccatttc
     5221 ccatgatcat ggtaaaaacc gccatggcaa tacaatagac tgcggtagct gcaaacagat
     5281 ttccggaagg aatcacagcg cttacgcctg cggcaattac atcgccgact cccgcagcgg
     5341 taaacacggc tccaagagcg gccagaacct gcgggagaat accggtgacg ccgacattgt
     5401 ctgtaagacg agtacagtcc tttgccgcat atacaggttt tgggcgaaag agcaggaaag
     5461 ccgcaataag tgcaattact cctgatacgc cgatggcgtt attggctccc aaagatttaa
     5521 aaaaggtagc gaccagtacg gcgctgaagg ccagaagaag aggcggaata aatacagaat
     5581 atcctaaccg gtctgccgct gcacgtgttt tggctgccgc gggaacgtcg ctgctgctgg
     5641 gggcaaccct tttaaatgca gtgaggatcg ccataaggac gacacatagt ccggtgatcc
     5701 atctggggaa atagggaccc gcaataaatg tagcggccag aataaaccag aaggccgcgg
     5761 ttgtaatccg tttttcacag gtcggatcct ttaaagcctt aaagcctgtg ccaataaaaa
     5821 taaggcctat aatacagtaa aagaattcag caactgcagt actgagagtc caggtcatat
     5881 tagtttgcct ccttttttct gcgtttatct ccgaatctgc gtttcaaaat ccggtcaaaa
     5941 agcagggcat agaccacgcc tgccaccaga gaaagtacgg cgatggggat tgactgtccg
     6001 gcaatctgaa gggcgtctac gggatagccc tgttcactta gggtggacac aatcagaagt
     6061 gtgccggaag aacccataaa gcagttctga gcaaaaaagt taccgtaatt ttcgcttcct
     6121 gagcacatac ccttaatttg atcttcagtc ttttcgtcca gtttgtcata ctggacaaca
     6181 gcggctgcct gagccatagg attgatcaga ggacggataa actggacatg gcctccgatg
     6241 cgaagagaaa atgccgatgc aaccgtacga atagcctgcc agatcgcaat cagacggccg
     6301 gttgtaacat ttttcaacga tttgataaaa tcgacagcct tatcctttaa gccattgcgc
     6361 tcgcacagtc cgataacggg cagggtcagg ataaaaagag tagctgtccg gttagtgata
     6421 aaggcggttc caagagtatc cagaatctcc agcggactca tgcctgccac cagaccggta
     6481 gcaagacctg ctgttaccac catggctaga gtgtccagct taagtataaa tcctgcaatt
     6541 acgatgagga cgccgatcag tttcacaatt tccatacaaa tctccctcct taataaatgc
     6601 gctgtaaaag cgctttttat ttttgacatc cggatgtgtc gataaaattg tcggagagga
     6661 acaacaggaa aatattctgt attcttcagc ggatcagact acacacataa taaaaaaata
     6721 tggtattatt aaaacagaga gaaacacagt acaaaagttg atttcaagga agggaacaac
     6781 agaatggatt gcataggaga aagaattttg aatgcatttt gcggcgcagt cgccgtttat
     6841 gatgaagaat gtaaaacgtt ttgttttcaa tattgtccgg aaaaagtagt gcagctctta
     6901 gggtatgata tgccgtattt tgatctgcat ttcagtaaag atgccctggg gcttttgtgt
     6961 gacgaggaca gaatggaagt ggagcatgca attatgtccg caaagtccag gcatatagga
     7021 atcaaagttt actctccggt caaagagaaa acaagctgtt taaagtggta taaaatagag
     7081 ggatgggagg atggaacaac atactgtctc ctttttgggg gaatgtccca ggaaacccag
     7141 ctgttccaga gcatagccag tgaaaatgca gacaatattt atgtgattga taaagaaaat
     7201 tatgatcttc tttatgtaaa tggttttaaa cgtgcattct ggaatgagga agggcagaat
     7261 acacctaaat gttatgagca tatttataat aaaaaagagc cctgtttcca ttgtatgatg
     7321 aagatgggcg ggtcagagga aatgccctgt gagacgagct atgaagaggg agggcgctgc
     7381 tatgctacac attttaaaga aacagagtgg aacgggacgc ctgcatatat aaaatatgtg
     7441 agggatatta ccgaagaagc aacggtgaaa agagaaaagg agcgtctgga aaaatatttc
     7501 gagacagttt tgaagtacct tccgggcgga gttgctgttg ttcaccatgg actgaatggg
     7561 agattgactc cggagtattt gtccgatgga tttgccgaga tggttgatat gtctatggaa
     7621 gaagcctggg atatgtataa gacaaatgcg ctttcaggtg tccatccgga tgatcaggaa
     7681 tatgtaaaac agacattaga ccgctgtatt cgtgaaaact gcgacagaga agcccttcaa
     7741 taccggctga agaaggggag cggcggttat atctgggtca atacaaaatt ttccgttatt
     7801 cagtgtgaag ctggggacgc catggtttat gcggattatc atgacattac agaagagaga
     7861 gaaatgcagg agagattaag gcttcagtac agggagcaga ttttccagca ttatttgata
     7921 acagatccca acacactgat tttagggcat tgtaatatca caaaaaataa gattattgag
     7981 attgaagacc gtaccaattc cggacttctc gaacggtttg gtgataaacg ggaggatttt
     8041 tttacaggaa ttggaactct ggtagcgtca gaagcggagc ggagcctgtt ttatgaaaaa
     8101 tatttaaatg aaccgtctat cagggctttt gaaaatggga taaaggaagt tctgatgcca
     8161 tgttttgtga agcttccgaa ttatccgcag ggaagatatg tacagtttaa ggtgaatctt
     8221 gtggaaaccc cggatacgag agatataacg ggtattttaa cggttacaga tattacggaa
     8281 aagacaatac aggacagaat catccgaatg ctgtcatctt taaattatga tctggttgca
     8341 gacgtggatt tgataaatga tcagtataaa gtcgtaagcg gtggggacca taatataaca
     8401 gaaactcagg gaagcctgat ggaacgggtg agacgtgtgg ttgaggagct tgtggtggac
     8461 tcggagaaag catatgtaag ggatatgctg gatccgggga agatggtcag ccggctgaag
     8521 gacggagaat tctattcgtt ccggtattct gtaaataatg gaagcaatga aatccagact
     8581 aaaaatatga ttatttccgc cattgatctg agtcttggca gaatctgcct ggtgcggaca
     8641 gacgtaaccg atatgcttgc tgcggaaaga aaagtgaagt atgatctgga aaaggcatta
     8701 tcagaggcag aaaaggcaag cagggttaag agtgattttc tgtcttacat gagccatgat
     8761 atccgtaccc ctatgaatgc tattgtggga atgacaaccc ttgcgcaggc caatctttcg
     8821 gattcccgga aggtggagga ttatctgaaa aagatttctg tatcttctca gcatctgctg
     8881 agtttgatta acgatattct ggatatgagc cagattgagc agtccaaatt gcatatgaat
     8941 gtgcaggtta ttcatattga tgaacttctg gatcagattt cctctattat ggcttcccca
     9001 tcaaaggacg cgggactgaa atttatggtt gagcggggag agatacagca tctgtatttt
     9061 cagggggatg ccttacggat aaaacagatt ttaatcaaca tactcagcaa tgcatttaaa
     9121 ttcaccatgg aaggcggaaa cgttcggttc aggacggagg aaataccgtc atatgaaagc
     9181 ggaagagtaa gatatcgttt tgtcatcagc gacacgggaa tcggtatgac acaggagttt
     9241 atctgccatt tatttgagcc cttctgccgc agtgaacggg tgaacagggt agagggaacc
     9301 ggtctgggac tgagtattac aaaggggctt gtagatctta tggggggtaa aatccgggta
     9361 gaaagccagc ttcatcaggg aacgatgttt gagattgaac tggaatttgc tgtggcagac
     9421 ggaaagcaga agcgttcccc aaatgcggat gaatgcatac cggaggaatc gctggaagga
     9481 agacattttc ttctggtaga agacaatgcg atcaattcag aaattctggc agaacttctt
     9541 cagatgtggg gagcaacctg tgttttgagg gagaatgggc tgcaggcagt agaggagttt
     9601 gaaagcagtg ttccgggaac atacgatgcg attttaatgg atatacagat gcctgttatg
     9661 aatggctatg aggcaacgcg caaaatccgc agttcgtccc atccggaggc gaagacaatg
     9721 gtgatattgg ctatgactgc aaatgcattt gcagaagatg tgcagaaatc cataagtgcc
     9781 ggaatgaatg gccatgtggc taaacccgta gatatgaaaa tactgtacaa taccttaatg
     9841 gcgttgttag atgattaaac agtgacaggg agaaagagat atgtacattg cagaccttca
     9901 cattcattcc agatattcca gagccaccag ccgtgagctg acaccggagc agttggatct
     9961 ggaggcggga aaaaagggaa tacatctttt aggaactggg gactttaccc atccggcctg
    10021 gcgtgaagag ctgtatgaga agctggtcag ggcagaggac ggactgtacc gcctgaagga
    10081 cgaatacagg tcagagaatg gacggcggtc agagacagct acgccacgtt ttgttgtttc
    10141 cggagagatc agctccattt acaaacagga cggaaaggtg cgaaaggttc acagtctgat
    10201 tcttctgcct gatttggagg atgcccagag gctgtcggcg aaactggaaa tgattggaaa
    10261 tatacattca gacggccgtc ccattcttgg actttcctgc catgatcttc tggaagtcat
    10321 gctggagatc tgtccagggg gaatgctgat accagctcat atctggacgc ctcatttttc
    10381 catgtttggt gcatgctctg gttttgaccg ggtggaggac tgttttgggg agctgacgcc
    10441 ctatatccat gcagtggaaa ccgggctttc ctccgatccg ccgatgaact ggcggctttc
    10501 tgccctggac cggttccagc tcatttccaa ttcagatgcc cattcccctg ccaagctggg
    10561 aagagaggca aatcttttga actgtgaaat gtcctatgcg gggctgaaaa atgccattga
    10621 aacaggagag ggattggaag gcacgattga gtttttcccg gaggagggga aataccattt
    10681 cgacggacac cggaaatgcc acatctgtct gagtccggcg gaggcgaaac ggtatggcgg
    10741 gatctgcccg gtatgtaaaa agaagcttac tatgggcgtt tctcatcgga tcgatcagct
    10801 ggcagaccgg cccgaaggct ttataccaga gggaagaaaa acgtttgaaa gcttggttcc
    10861 tcttttggag gtcattgctg cgtctaccgg acggtctgca gcgtcaaaga aggttcagga
    10921 agaatactgt aaaatggtgg atgagctggg aacggaattt gatattttaa gaacgattcc
    10981 gattgaagaa atcagaattc attctggtca tctgatttct gaggggatcc gccgtctgcg
    11041 ggaaggccgg gtaaaacgtc tttcgggctt tgacggagaa tatggtatca tccggctttt
    11101 tgaaccggat gaaattgcaa atccggacgg atagatgagt ttcaggatat cagccccatt
    11161 cagtaccgtc tgatccgtga atggaaccgt gatggaaagg aactgtttgt gatcggtgat
    11221 ccggcagatg aacctgtttg attttatata gaaacccaga gagggctggt attgacattt
    11281 ttccctctgg atgctataaa taaaaccata ctaagttact atgaatagtg tgcaaacgta
    11341 acacccagga ggacattatg gaaaaatata ggaaaattgc aggaaaatgt cttgcagcag
    11401 ttctctctgc tgccatggcg ctgtccttgg cagcctgtgc aggaggtacc ggaagcagcg
    11461 aaagccagag ccaatctgcc aaacaggagg cagctgcaga aaagcctgct caggccacag
    11521 gggaaaagat tgtaaatgtg ggagtaacag gaacagtaaa taccctgaat cccctcctga
    11581 tggacggcgt ggagatcaat aaatatgcca ccggactgat gtttcttccg ctggtggagc
    11641 tggacaccaa tatggagttt gagcccatgc tggcagactc cattaccgca gaggacgaga
    11701 aacattttct ggtacacatt gacgatgagg cggtctggtc tgacggccag ccggtaacag
    11761 ctgatgacgt agtatacaca gctctgcgcc tgtgcagtcc ggccattgcc aacacagcca
    11821 tgatgtatta tgtctttgaa ggcgttggtg atgacggttt tgtggaaatg ggagcagacc
    11881 atattgaggg aatcacaaag gtggatgata agacggtgcg cttcgccaca aaggagccca
    11941 tgtccttagt tacctttcag aattcctatg cccggtatct gatgcctctt ccaaagcatg
    12001 tgatcggaga gatccctgag gcagagctgg cctcccacga gtggtttgca aagccggatg
    12061 tagtaagtgg cccttaccgt gtcaccgcct ttgacaagga tcactatgta tcctatgagg
    12121 cgaatgagaa atactggaag ggagcgccta aaattccgaa gctgaatatc cgtattgtag
    12181 agggaagcca gctttatgcg ggacttcagt ccggggaaat cgatatcaca cagaacacca
    12241 tgagcgccat tccgctggag gattatgaga gccttcaggc gctggaaaca gtggagacaa
    12301 gcttcggtga cgctattacc aaccagtctg tatttatcaa tacaaaaaat gtgccggatc
    12361 agaagttccg ccaagctctt ttatatgcca ttgacagaaa tcagatttta gagcagctgt
    12421 taaagggcaa tggagaagtg gtagacggct tcctttccac cgcaagccct tactacgatc
    12481 cgtccattga gcctgtttct tacgatcctg caaaggcaaa ggaacttctg gcagaaagta
    12541 actggatgcc ttcttctccc ctgcgtttct atattgattc cggagacagt acctttgtaa
    12601 atgctgcctc tgtaatcgca gcccagtggg cggctgtggg aatccagacg gagatccgta
    12661 ccatggatat caatacgctt atgagtactg caggaagcgg agactttgac attatggcgg
    12721 tgcagtacac ctatccgcct gtagatcctt atgtagatat tgcatggctt ttaggcggcg
    12781 agggaagctg gaccggttac agccacgctg aggcagagga ggctctttta aaaattgctg
    12841 tattaagcga taaggaagaa ttaaaagagc agtacggtat cattgaccgc cggatgcagg
    12901 aggatgtgcc catgttttcc gcgtatgtga tccgcaccat ggcagcgaaa aataaacgcc
    12961 tgaccggagt aaacccctcg gtatacggct tctttaatca tgtggaagaa tgggatatca
    13021 caggctgagt gtgatcccgt ctgccgtacg tgaccataca ttgttactcg ttccccggca
    13081 aagctttttg ccggggaatg tcggttcatt acaaaatctg aaaaaggagt taggaatgtc
    13141 acatattttg gaagtccgtg gcctgcaggt tgcatttgca ggggaacgcg gaaataccat
    13201 tggcacagac cggattgatt tttttgtgga tcagggagag acggtctgtc tggtagggga
    13261 gtccggctgc ggaaaaagta ttacatctct ggctgttatg gggcttttgg gccggggcgg
    13321 ccgtgtgtca cagggagaaa tcctttttaa tggaagaaat cttttgaaaa tggatcggaa
    13381 ggagctggat caggtcaggg gaaaagagct gtccatgatc tttcaggatc cattaagctc
    13441 cttaaacccg gtcctgacca ttggaaaaca gctggaagag ggcttaaagg cccacacaga
    13501 gctgggaaaa gaggagcgca ggaaacgggc ccttgatctg ttgggcagag caggaatccg
    13561 ggaagcagag gctgtgatga agaaatatcc ccaccagctt tccggaggaa tgcgtcagcg
    13621 tgtgatgatc gctatggctt tatcctgcca gccttctctg cttattgcag acgaacccac
    13681 aacggctctg gacgtgacca tccaggccca gatcatgaat ctgctgcgtt ccctgaaaag
    13741 ggagcttcgt atgtcgttgc ttttgattac ccacgatatg ggactggtgg ctcagatggc
    13801 agaccgggtt atggtgatgt atgcggggca gatcatagaa gaagggacgg tatttgagat
    13861 ttttgaccat ccgtcccatc cctatacaaa ggcgctgctg gctgcagttc cgtccatgga
    13921 gcagaatccg gagaaaatgc ttcaggccat agaggggacg gttcctgaaa attacgggca
    13981 gatgaccggc tgccgtttca gagaccggtg cccttaccgg aagcagggct gcgaagcgcc
    14041 ccaggagctg gaagaaataa aggcagagga aacacagaag gcacaggagc ttcaccgggt
    14101 gcggtgctgg cgggcaaagg aggcagagca aagtggcaga accttataag aatacagagc
    14161 ccctgcttaa ggtggagcat ctgaaaaaat attatcccca gaaccggccc attttcggcc
    14221 gtcccacctc atggatccgg gcggcagatg atgtttctct ggagctttat ccgggggaaa
    14281 cactggggct ggtgggagaa tccggctgcg gaaaatctac tctgggccgt atgatcgccg
    14341 ggctggaagc cccggactcc gggtgcatct ggtataaagg acaggaactg actgcaaagg
    14401 gaggcagagg ctatagcgca gtgcgcacag agatccagat ggtatttcag gattcctatt
    14461 cctctttaaa tcccagaaaa cagatctttc agatcctttc agaacccatg ctttaccaca
    14521 gcctggcgga taagcatacg gttcataaga aggcagagca tctgatggag ctggttgggc
    14581 ttcctctcag cgctctggac cgttaccctt atgagttttc aggcgggcag cggcagagaa
    14641 tcggcattgc aaaggcgctg tcactggagc cgaagctttt gatctgcgat gagccggtaa
    14701 gcgctctgga cgtatccatt caggcccaga tcctgaatct gcttaaaagc cttcaaaagg
    14761 agttagacct gacctgtatt tttatcggac atggattagg ggctgtaaat tatgtaagcc
    14821 ggcgtattgc ggtgatgtat ctggggaaac tggtagagac gggggagagc agtgagctgt
    14881 ttgagcatcc ggtccatccc tataccagaa cccttcttgg ggcagtgccc atcccggatc
    14941 ccagaaaaag ggaggaaaac tggggcgaga ccagtgaagg aacagcgtat gagccgtcca
    15001 tcacaggggc agaggataag ggctgtccct ttaaagaccg ctgcttcagg aaaaggccgg
    15061 aatgcagcag cgcagaggta ccgcttcgtc ccgtccgtcc gggcagcacc catctggcgg
    15121 cttgtagtcc tgctgttttg gaggtaaaaa gaaacgaatg aaaaaatata ttataaaacg
    15181 gcttctcatt gctattccta ccctcatcgg gatcacgctg attgattatg cgatcatgtg
    15241 ctttgcagga agtcctctgg aaatgcttca gggcgctcgt atttctcagg aggcagtagc
    15301 tgcaaaggaa gcggctctgg ggcttgataa gcccttctat gttcagtatt ttatatggct
    15361 gggacaggtt cttaagggaa atctgggtgt ttccatcaag acctaccagc ccgtaagcca
    15421 gatgatcatg gaacgtctgg ggcccaccct gttactgatg ggagtttcac tggcagtgag
    15481 ccttcttatc agtatccctg cgggaatata cagcgcagtg cgccagtatt ccaaaggaga
    15541 ttatgcggtg gtaacagcgt cctttttaag cagcagcatc ccgggatttt ttctctccct
    15601 tcttctggtt tacattttta atattaagct taaactcctt ccgtccagcg ggatgattac
    15661 cctgggaggc tccggaggga ttggagatct ggcagcccac atggtgatgc ctgtgaccgt
    15721 tttagccctt tccatggcgg gaacaaatat ccgctatatc cgcagctcca tgctggagat
    15781 ccttcaaaag gattatctga ggacagccag ggcaaagggc ataggggaac ggctggtaat
    15841 aggccgccac gccctgcgga acggccttgt gccggtggtg acagtgatcg ggatgcagat
    15901 tccccttctg tttggaggcg ctgtgatcgt ggagcagctg ttttcctggc ccggacttgg
    15961 acttatgacc atgagcgcta ttttatccag agattatcct gtgattatgg gcgtctgcct
    16021 cttagccgct gtggtggttt tggcagcgaa tctggttaca gatatcctct atgctgtggt
    16081 tgacccttcc attcagtacg attagcaaag gaaaatgaaa tgaaaaagca aatgaaaaat
    16141 catgagacct atgcgggggc ggtatggcgc agattctgcc gccaccgtct ggctttggca
    16201 agtctggctg tgctggttct gatctgcggc agcgccgtct ttgcgcctgt gatcgctccc
    16261 tatgatccgg aggcaatcgt gggacccttc ggagcacctc ccggagatga atttctgctg
    16321 gggacggatc agattggccg cgatatgctg agccgccttc tatatgcagc ccgaacctcc
    16381 ctttttgcag gcgtactggc tactctgatc gctactgctc tgggcgtgtt tctgggcctt
    16441 ctggctggat atttcggggg cttggcggat atcctgatca tgcgttttac ggatatggtt
    16501 atgtcatttc cctatattct tctggtttta gtggcagccg ctattttcaa gcccggtttg
    16561 tggagcatta tcctgatact ggggtttgta gactggccgg gaattgccag actggtgcgg
    16621 ggaaacgtgc tcagcttaag agagacgaat tttgtaaaga gcagtgaggc ggcaggcatg
    16681 cctgctttcc acatcctgtt ttcagaaatc cttcccaata ccatggctcc tgtgctggtt
    16741 tatgccacct ctgttatggc ccttaccatg ctggatgagg ctgctttaag ctttcttggc
    16801 atgggggtgc agcctccggc cgccagtctg ggaaatatgt taaacagcgc gcagtcggtt
    16861 accgttctta caaaacagcc ctggctctgg attcctccgg gacttgtgat aattatcgtg
    16921 gttacagcgg taaattttgt gggagacgcc ctcagagatg cctttgatcc cctgtccata
    16981 ataaaaatgt aacagaagat acgcgggaag agcaaaaaat ctaaaaaaaa ttgtataaaa
    17041 attgaaacag aatgtgttaa aaaaatgtca agttaaaaag acgtaaaggc aaaacaaaca
    17101 aaaaattgca tgataatatt ataaaaaatt tagaaactcc ctaaatacca ttgataaatc
    17161 catgggtatg gagtataatt cactcatatt gtacaaagaa agtaaggaca tgacaaaaaa
    17221 aactgtgtct gatatggaaa aatatgccaa aggaatttgt gcaatatgac agggagtgca
    17281 caaaataatc ctataggcaa ccaaaaaaat ttaggtacgc aggatatgac aggaggatca
    17341 cacatatgaa gggatgggcc cataccatga gttctctttc tatgttttcc cagctgggat
    17401 tgtccctggc agcccctctt cttctctgtg ttcttatatg cagctggctg accggacgtt
    17461 ttggtatcgg gacgtggatc tatattcccg gttttttctt tgggctggga ggctctggag
    17521 ctacggcctg gaaggtctac cagaacatta tgaagcatca gaaaaagaaa aaaagaacca
    17581 atgagagggc atttaacgaa catttttaag gaggaatgag gatggcacag attcagccgg
    17641 ctgtcaaaaa ggagacagcc cgtgtgacgg ggattacttt gacagggtgc gtcattatgt
    17701 ttgcagcttt ttttgttctt cataagattt cacctgcatc agttcccttt gatatgaagg
    17761 tcattataag cggcctttta ggcagcggag tggctgtggc caactttttc tttatgggac
    17821 ttacgattca gaagatcgtt tccggcgagg atgaggaaca ggccagaaaa gatatgaagg
    17881 ccagctaccg gttccgcacc gttacccagc ttctgtgggt ggtgcttgca gtagcccttc
    17941 cgtttctgaa tggtgcagca gggcttatcc ccctgttttt tccaagcgca ggcatcaagg
    18001 tgctgagtat gtttggaatg ataaatgcaa aaccaaatcc atagaaagga gatgaaactg
    18061 caagtatgaa ctttgatatt tcaggtgcgg ccatatactt aaggattccc acaggaattc
    18121 ccgttctggg tgattttgtg atcactgaga ccatggttgt cagctggctt gtcatgatcc
    18181 tgatcacagg cttttgtatc tggcttacca gggatctgag ggtggaaaat atttccaaac
    18241 gtcaggcggt agcagagatg ctggtagagc aggccactaa tctggtccgc aacaataccg
    18301 gcggtacaaa gttcgactat ctgatcccct ttgtggcagc cctctttacc acaagtgcgg
    18361 tgtcaaacct gatcagccta acgggtctta gaagcccgac agccgatctt tccgtcgaag
    18421 cagcctgggc ggtagtggtc tttgttatga tcacagccaa caagattaag gcaagcggtc
    18481 ttctgggata catgaagggc tttaccacac cgattccggt gatgacgccg ttcaatatcc
    18541 tttctgagat cgcaacgccc atcagtatgg cctgccgtca ctttggaaac attctttccg
    18601 gcgttgttat caacggcctt ctttacgcgg ctctggccgt ggcaagctct gcactgcttg
    18661 gctggcttcc gggcgcgctg ggagaaatcc tgggaagcat tcccatcctg gatgtgggta
    18721 ttcccgctat tctgtctgta tactttgact ggttctccgg tctcatgcag gcgtttattt
    18781 tcagtatgct gaccatcatg tacattgcca atgcggcaga aggttaagca gacttatttt
    18841 actcagaaca tttattttcg aggaggaaaa ttatcatgac agattttcag gtattagcaa
    18901 aaggtatcgc tttagcagga tgtgccatcg gcgccggctg cgctctgatc gcaggtatcg
    18961 gtcctggtat cggtgaaggt aatgcggttg caaaggcgct ggaggcgatc ggccgtcaac
    19021 cggagtgcaa gtctgacgta accagcacaa tgcttcttgg atgtgcgatt gcagagacta
    19081 ctggtattta cggtttcgtt acaggtctgc ttttgatctt cgttgctcct ggcacattca
    19141 tgaatttcct gtcttaatgg aaaagggaac accgtattcc gcagacagga ggtaggaaga
    19201 tgagagagac acaggaactt gtaaccatcg taccctggac cttcattgct acgatctgca
    19261 acctgttcat tcagtgctgg ctgattaaaa agtttctgtt caagcctatc cgcgaagtga
    19321 ttgaaaagcg cagggctctg gcagattctc agattcagga cgcaaagaag gcaaaagaag
    19381 aggcagagac catgaaggcc ggatacgagg cagagatggc aaaggcaaaa gaaactgcca
    19441 gcgtaatcct tcaggatgcc cagaaggatg cagctgcccg cagtgaggct atgctgcagg
    19501 aggcaaaggc tcaggcagcc ggtattaagg caaaagcaga agccgatatc cttcaggaga
    19561 agaagaaagc cgttaatgag atcaaaaatg agatcggcgg cattgccatg gatatcgcag
    19621 gaaaggtgat cgagcgcgag atcagcgagg acgatcacag gaagctgata gacgagttta
    19681 ttgagaatgt aggtgaggca tcatgacaaa aactgccaga ctctacggag gcagcctcta
    19741 tgaccttgct tcagaagaaa agctgacaga ggaaatgtta gagcagcttg acatggttcg
    19801 ggcgcttttt aaggaagaac cctcttatct gcggctcctt gcagaacctt ctattccaaa
    19861 gacagagcgt gtgggtctga tccatacggc ctttgaaggt aagattcatg tatatctgct
    19921 gaatttcctg aagcttttat gtgaaaacgg catgctccgg gagttttcag actgctgccg
    19981 ggaattcaga acccggtaca atgaagacca tggaattgct gaggcagtgg tcaccagtgc
    20041 ggtgcctctg acagaggaac agaaagaggc actcaaagcc agactggaaa agattagcaa
    20101 taaaaccatt cagcttaccc ttaaggttga cccgcaggta gtgggcggca tcagggcaga
    20161 gctggaggga aggcagcttg acggcacact gcaggaacgt ctgtcaggac tgcgaaagaa
    20221 agttacggaa atcatcgtat aaaggatgtg aatggaatgc aattaaaacc tgaagagata
    20281 tccaaggtca tccgcagcca gatcaaatat taccagaatg ccattgagca gagcgagacg
    20341 ggaaccgtcc ttatggtcgg cgatggtatt gccagagtca gcgggcttac aaactgtatg
    20401 tccggcgagc ttctggaatt tgaagacggc accttcggaa tggcccagaa cctggaagaa
    20461 acaaatgttt ctgttgtatt attcggatct gaccagggga tcagcgaggg acagacagta
    20521 aagcgcaccg gaaaggtcgt atctgttcct gtaggcgagg ctatgatcgg ccgcgttgta
    20581 aatgctattg gtcagcccat tgacggagca ggccctattg aaacaagtga atacagaccc
    20641 gtagagtctc cggctcccgg tatctgcgac agacagcccg taaaggagcc tctccagaca
    20701 ggaatcaagg caattgactc catgatcccg attggaagag gacagcgtga gctgatcatc
    20761 ggagaccgtc agaccggtaa gacaaccatt gctacggata cgattttaaa tcagaagggc
    20821 aaggacgtta tctgtatcta tgtagccatt ggacagaagc gttctaccgt tgcaaacctg
    20881 gtatctgagc tccaggcagg cggcgcgatg gattacacca tcgtagttac ggctactgct
    20941 tctgaaacaa gccccttaca gtacattgcg ccttattccg gctgtgcgat gggagaatat
    21001 tttatgaaca agggaaaaca cgttctgatc atctatgatg atttgagtaa gcatgcagtt
    21061 gcatacagag cactttccct gctgatccgc cgtccgccgg ggcgtgaagc ttatccgggc
    21121 gacgtattct acctgcactc cagactgctt gagcgtgcag caaagctttc tgatgaaaag
    21181 ggaggcggtt ctctgacagc ccttcctatt attgagaccc aggcaggtga cgtatcagcc
    21241 tatattccta ccaatgttat ttccattaca gacggacaga tcttccttga gacagagctg
    21301 ttccattccg gcattatgcc tgctgtaaac ccgggtattt ctgtttcccg agtaggagga
    21361 aatgcacaga tcaaggctat gaagaaggta gccggtacct taaagctggt ttactctcag
    21421 tacagagagc ttcagagctt tgctcagttc ggttctgacc tggatgcgga taccaaagcc
    21481 cgtctggctc agggcgcccg tatcgtagag gtattaaagc agggcagaag tacgccggtt
    21541 ccggttgaga agcaggtagc aatcctgtat gccgttgtaa acggtatctt aactcaggtt
    21601 gaggtagagg atatccagtc ctatgaagag ggactttaca ccttcttaga cagtgatgct
    21661 gcaggtgtgt ccgctatgga aggaatccgc tctacaggta agctggaggg cgagacagaa
    21721 gagaagttaa agacggctct taaggattac acagaccgtt ttttaaaggc cagataatca
    21781 gataaaagga ggaagaccat ggctgcaaat atgaaagccg tgaagctccg tatgaagagc
    21841 atacagagca ccatgcagat caccaaagca atggaactgg tggcatcctc caagcttcgc
    21901 agggccaagg aacgggcaga tgccagccgt ccctattttc atactttaag ggatacgctg
    21961 gctgacattg cggcaggcaa tacggacttt tcatcggttt ataccaggtc cggaaaaagc
    22021 ggccgctggt gttatgtagt agttgccgga gacagaggtc tggcaggcgg ctacaatgcc
    22081 aacctgttta aagagatgga agcgcagatg gcaggaaagg actgtattgt gcttcccatc
    22141 ggaaaaaagg cggtagagta ttataagcgc cgtaaggtag agattctcac agaagagttt
    22201 gctgaggtgg gagatctgtc tgtttccgac tgctttacta ttgcaaagct tctttgcaaa
    22261 ggctttaaat caggcgactt tggccatgcg gcactgggct ataccaattt tatttccatg
    22321 ctctcacagc gtcccggcgt atccggcatg ctgcctctgg aggatttctc cggggaagag
    22381 aaggatgtaa aaaagatccg ttctctgatt ctgtacgagc cggatgcgga aactgtattt
    22441 gactccattg tgccggagta tctggcagga cttctttacg gagctatgtg cgagtctgta
    22501 gccagtgagc tggctgccag aagaaccgcc atggacgccg ctaccaagaa tgccggcgag
    22561 atgatcgaac agctgagtct ctactataac cgcgcccgtc aggcaagcat cactcaggag
    22621 atcacagaga ttgtggcagg agcggaggag ccctgatctg cgggatagac ggccccgtga
    22681 gtatctgtgc ggggatgata tgcatcattt tttggcagaa catgccaaag ggtggataac
    22741 aaggagattt gtatatgact gagaaacata taggcaaggt agtgcaggta acgggtcctg
    22801 ttctggatat ccgtttcagg gaaggagagc tgcctgcgct gctgaatgcg attgagattg
    22861 atgtaaatgg tcatcgcctg gtggcagagg ttgcccagca ggttggtgac gatgtagtgc
    22921 gctgtattgc catgagttct acggacggac tggtgcgcgg tgcagatgca gtagatacgg
    22981 gaagtgcgat taccgttccc gtaggcgatg agtgtctggg acgtgtattc aacctgttag
    23041 gtgatccggt agatgagaag cctgctcctg aggatgtgga gcgctgggct atccatcgtc
    23101 cggctccttc ctatgaagag cagatgccgg ctacagaaat cctggagaca ggtatcaagg
    23161 tagtagacct gatctgtccg tacgcaaagg gcggtaagat cggtctgttc ggcggtgccg
    23221 gcgtaggaaa gacggttctt attatggagc tgatccacaa cgttgctacg gctcacggcg
    23281 gcctgtctgt atttacaggc gttggcgagc gtacccgtga gggaaatgat ctttacggcg
    23341 agatgaagga gagcggcgtt atcgacaaga ccgctttggt atatggtcag atgaacgagc
    23401 cgccgggggc tcgtatgaga gttgcccttt ccggcctgac catggcagaa tatttccgtg
    23461 atgtgaagaa tcaggatgtg cttctgttta ttgataatat tttccgtttt acccaggcag
    23521 gctccgaggt atccgctctg ctgggacgta tgccttccgc ggtaggatat cagcctactc
    23581 tggctacgga gatgggcgcc ctgcaggagc gtatcacctc tacaaagaag gggtctatta
    23641 cctctgttca ggctgtatat gtgcctgcgg atgacctgac tgacccggct ccggctacca
    23701 catttaccca tctggatgca actacggttc tttccagaga tattgccagc cagggtattt
    23761 atcctgctgt ggaccccctt gagtctacca gccgtatcct ttcaccggag gtagtaggag
    23821 aggagcacta tcaggtggca agatctgtgc agagagtgct tcagagatat aaagaactgc
    23881 aggacattat cgcaattatg ggtatggatg agctgtcaga ggatgataag ctgactgtat
    23941 ccagagcaag aaaggtacag cgcttcctgt ctcagagctt ctccgttgca gagcagttta
    24001 caggtatggc aggacagtat gtgccgttaa aggaaaccat tcgtggcttt aagaagattc
    24061 tggacggcga gtgcgatgag attcccgaga gctgcttcct gtttgcggga actattgacg
    24121 atgtctttga gaaagcgaaa aaggcacagt agaagggagg gtgaggcttt atggcagcct
    24181 tccatttgca gattgtgact atggacggaa atgtctttga cggacaggtt cagagcgttt
    24241 cctgccgtac gatccacgga gatctggcga tcatggcccg tcatatgaac tactgtacgg
    24301 cagtaggcat gggaaccgct catgtgataa tggaggatgg tacagagcgc acagccgcct
    24361 gtatcggcgg tatgctgacc gtgatgaacg gagagtgccg tctgatccct actacctggg
    24421 aatggagcga tgagatcgat ctggagcgtg caaagcgtgc aaaggcacgg gcagaggaaa
    24481 agatgaacaa gtccggcctg tctgaggtgg agcgtcagat gatcgaggca aagcttcaca
    24541 gggctctggt ccgtatcgga actgtggaaa agtaaaatag attatagttt gattaaaaat
    24601 ggccgccgca aggcggctgt tttttgttat ggcggcgatt gatactttaa actaattgtt
    24661 tttccatttg ctgcgtggta gaatgaggga aatacaggaa agaaaacaga ggtacagatg
    24721 tgatggagac aaagagaatt tatataaagg atacagaaca tgtaagggat gaggagcttt
    24781 tggaggcggc agaaatcatc cgccggggcg gtcttgtggc ctttcctacg gaaactgtat
    24841 acggattggg aggaaatgct ctggatgagg atgctgcaaa aaagatttat gcagccaagg
    24901 gccggccttc tgacaatcct ctgattgccc atgtttcctg tccggaagaa gtagaacctc
    24961 ttgtagaata tatgcctgag gctggtaaga agctgatgga agccttttgg ccggggccgc
    25021 ttaccatgat tttccccaaa agcagccggg ttccctatgg aaccacagga ggtttggaga
    25081 cagttgcggt gcggatgcct tccgatccgg tggcgaaccg attgattgct ctggcaggag
    25141 ttcccattgc cgctcccagc gccaatacct ccggtcgtcc cagtcccact acagcagatc
    25201 atgtatggca ggatatgaac ggaagaattg atatgatcat tgacggaggc cctgtgggga
    25261 tcggcgtgga gtctaccatt gtggatgtat cgggacaggt gcctgtggtg cttcggccgg
    25321 gagccattac gatggaaatg ttaagagaga ctctgggaga ggtgacgatt gatccggcta
    25381 ttttaggccc tatggcagaa ggcgtgcgtc ccaaggcgcc tggaatgaag tataaacact
    25441 atgctcccaa ggcagagctt actttagtgg agcctgcgat ggaggacaaa aaggcttcca
    25501 tgactcagga acaggtagaa cgcatggttt ccatggtatg gaaactggca aaggagcaga
    25561 ttgacgccgg agtgcgggtg ggaattatct gtacggatga gtccagagct gcctacccgg
    25621 agggcattgt ccgcagcatt ggagcaagaa aaagccagga atcggtcgct cataacctgt
    25681 acggagtact gcgggaattt gatgatctga aggcagaagt tattttttcg gaaagctttg
    25741 gggaagacca tctggggcag gcgattatga atcgtttgtc caaggcggca ggatataaga
    25801 ttgtgcgtgt ataagagggg gaaaatacat ggaaaaacag accctgggtg aaaagacagc
    25861 ccagatgctt ttggaaatga tacaaaagga gggctttggc ccgggggata agcttccgac
    25921 ggaagcagag ctggtggaat ctctgggagt gggacgcaat actgtccggg aggcgctgcg
    25981 catactgatg tccagaaata ttgtcaccat ccgtcaggga tccggaacct ttatttctga
    26041 aaaaaaaggc gttgtggatg atccgctggg tttttccatg atggaggacc ggcgcaggct
    26101 gaccgaggat ctgatccagg tacgtctgat gctggaacct cagctggcag cactggcagc
    26161 ccagaatgct gccgaagagg atttacagaa actggaacag atcaaagagg agctggaaga
    26221 tctgatccgt cagagaaagg attattcctt aaaggattca cagtttcatg agcagatcgc
    26281 taactgcagc cacaaccaga tcatgaccaa tctggtgcct gtgatcacag agggcgtgcg
    26341 gatgtttgcc gtctcggttc aggaaacgga atatgagcag acactttctt cccacaggag
    26401 gatctttgag gccatccggg agcggagagg agaggatgca aagatggaga tgtatttcca
    26461 tttgatgtat aacagtaatc ggtatcgggc ggaaaccgaa ccgtaaggaa aaagcgtgtg
    26521 ctttcttttg ttggagaagc acacgctttt ttcagatcca gcgtttcctt actgaatcca
    26581 tctgccgttt tcatctacct gataaccgtc aggagttgtg gcagaagtat acagttttcc
    26641 catggtaccg tcagaaacgg ggttaaaata ataccagttg ttttcgattt gctgccatcc
    26701 ggttttcatg ccggaattga tatcaatgaa aaaccagctg ttctgctcat ggtcaaagaa
    26761 aaaaccggat tttgtgtatc cgtcagcgcc aaaggcatac caggccccgt tgatcatttc
    26821 ccatgcgggc tgttcatata cagtgccgtt ttcagctgtt tttatggagc ctgcggcaaa
    26881 tgttccatct gcatactgaa gcttccactg agatggattg ccggattcct gaatccaatg
    26941 actattgttt gttccgtttc cggttataat tccagatacc gtatttacat atcctttttt
    27001 agaatcaagg attgtgtggc tggatacaga ggagccgggg ccgcttacag aggttccgga
    27061 accggaagag ccgtcagaat cgctattatc cgggatagaa ggaatttccg gtacgttttc
    27121 atcgtttaca gtaactgcag aagaggcaga ctggccgccg gcagataccg taattacagc
    27181 ctgaccggga cgcagaccgg ttacaagacc attttcatct acagaggctg tagagggatc
    27241 ggagcttgtc cagctggcag tgaggcctgc tggtgtgatt tctgcatgta gctggatgct
    27301 gccgcctacg gcgatccgtg cattctgagg agacagggat accgtaactt cttccctgtt
    27361 ccatacagcc tgaggtccgc tgtaggtcca atggaaggga cctgtgccgt tcagcgcctt
    27421 ccaccagtta tcggctgcct ctactttaaa ggtatgagag gtttcaagat cagctcctga
    27481 aattcgatag ctggtttttg tggtggtaca actgctgttt acatggttaa agtagtcgtc
    27541 ctcatctgcg tagacaggtt ttccgtccat ataaatacgg tatccgtaga ttcctgattc
    27601 atcccacggt tcctcccagc tgagaatgag atctccattt tcttcgcgaa cgtccaggct
    27661 gctgtcccat tcgggagcct tgaaatcata tttatgtgag agctttaccg tttcagattc
    27721 ataatcaaag gtatcgccat tatcagcaaa agctgtaatt ataacctcgt tttctgtttc
    27781 cataagacgc ccgatggtaa gggtaagttc atcctggctt gatttgctgc cgagctcata
    27841 attgtagatt gtctttacaa gtactccgtc tgcataggca tgatatccgc ggatgcgcgg
    27901 atctgctttg ctgccattgg caaaggtggc agaggcccag gtatatcctt cgccggaata
    27961 gcgtacctta cggtcagagg gaacgtttaa tgtttcatcc tgtgcagatt caattctcag
    28021 gcctgcagag ggaccttcgg tcttatttcc ggttgcatca acggcataga cagtaaaggt
    28081 gtaatcgaca ttggcacaaa ggccttctgc ctgatattcg ggtttcgtta caggcgtgga
    28141 aatatctaca agctgtccgt ctgcccgtgt ttcgatgata tagccggaga tttcatcata
    28201 gtcagcggcg tctgtccagg tcagctccgc tgcttcgtct gcggaagcgc gggaagcgtt
    28261 agatggagag gctgtaagta cggcatcctc tgtccactcc ggaatatcca tggcattctc
    28321 cgctttatca gaggttgtgg tatcgcctgc aaaattcagt ccgccggatg cggagatctt
    28381 gctccaagcc ttggaatctt catctttatc attaaacctt acattataaa agacggaatc
    28441 ttccacgccc ttcagttcct gggccatttt actgttggta aatgtaatat cctggaaata
    28501 aaggtcatgg tgggtgtgcc agggcttttc aagctggctg taatccacat cagctaccag
    28561 gctgaactca tttttcttta cgtcatcaat cgtaagatta taggcgttga tattgcggaa
    28621 ttcagctggt gtatcagcag gctccacaga tacagcctga tttacatcgc tgtaggttgt
    28681 gctcattaca aacgcctggc tgcattctcc cactgcacag tcgcggatca gcacatccca
    28741 tacgcctcct ccgttggcag gagcagattt gaatcggaat ggcatatcgc tgtggtttaa
    28801 aacattatct tctaccagca tattacagat accggctcct gtatggcttc cggccgcaat
    28861 agcgccgcca tgcccttctc tcaggaaatt gttaaaggtc cagatgttct gggaaggctt
    28921 ctgttcgcag tcctgaacgc cttttcccat accggtagca aagttgatag aatcatcacc
    28981 cgtatcaaag aaattattaa atacaatggc attctgtgta tttccaagtt caataccgtc
    29041 tgcattgttg gaatcatagg tggtatattt tacattattg gaggttacat ttttactgtc
    29101 aaggacagca accgtgtgga aggcaggatt gcggactgta atatcgccaa tataaacatt
    29161 ttcagttccg cggattacaa tcaggttagg gcggttattg taggccacct tttcgcttaa
    29221 tcctttttct tttgccttca gggtagcgtc tgaggccaga ataccaaggg aggtttcaaa
    29281 aggtgtggta tatagctttt tggagtttcc ggatacccag cgtgggaggc ggtaagcctt
    29341 atcctccgga tcagctgcct ggcgatcctg atagaacata gagtagccgt ctccgttgat
    29401 atcatccttt tcgccatatt tccagccgtt tccatcaatg gttccctctc ctgttatgcg
    29461 gatattttcc agcataccgc cctcatcgct gctgtaagca ttaatcagag cccaggatct
    29521 ggtatctgta gagtagggat acaggagata attgctgtca tagtggtctg catttgggga
    29581 gccaaagaga acagcgccct tttcaagctc cagtgtcata ttactcttca ggtacagagc
    29641 gcctgacatg aatataccgg aaggaatgac aacctttccg ccttccgtgc aggcatcgat
    29701 ggcagcctgg attgcttttg tattttcttc aatgaaccgg ttgatcccgt catcatagga
    29761 acgataggga gtatcaaccg tacgggcgcc aaaatctgta atattaaata tttccggggc
    29821 aggagctgtg gaggcggaaa cagaagccgt gtctccaatc ggatttccgg aatcatctag
    29881 agccacgacg gaaaattcgt aggaggtatc cggcgtcagg ttatctgccg taaaggagtg
    29941 aatatctacg tttaccattt cgatgttctt tttttcatag tattcgtaga atgcgtccat
    30001 gtatgtactt gcccagtcgg catgttcggc gaaattttcc cgggctgttc cgatgagctc
    30061 accgtcctga tatacggcgt agtctgcgac agtatcgtat ttttcaggct tattccatac
    30121 cagcgtcaca gaggtgtcgt cataggccag agtgggaacc agcagattgg ttacgtcctc
    30181 gctgccttcc gtacggtcat atttgggttc tgtcagggga ggcgttttgc ggcgttctat
    30241 ttttggggcc gcatttgacg gagtagaaac agcgtcagag ggggaagcgg gacgtttatg
    30301 cgcatctgat gaggttgcat ctgatggagt agcgattatt tcattgtcct catcccggtc
    30361 ttcggaggaa ctgtgttcag catctgatgg agtagctaaa ggctgatata tgggggaggg
    30421 ggtgaaatca tcgcgattgg catcagaatg ggaagcagta aggctgttct gagaacttgc
    30481 aaacgtttgc aaatcggggc taaaaagaat tgcacccgtt gttaacgtac cagcaagagt
    30541 tatggatgca taacggtata gattggacgg aaaatggatc ttcataactg aattcctttc
    30601 gagttacttt ggacgcgtta ccaatatttg tatacagata aaagtataac taatgaaaaa
    30661 ttatttgtac actgttaaaa gtaacagaga aaatataaaa tatttgtgaa aaaattataa
    30721 agaaaaaaat ggggagttat gaaaaagtca caaaaatttt tgagtaaatt taatgaaaga
    30781 gtcgatagaa aaaatacaaa tattcgtcct atgaatattt gataatatct caaaaaatat
    30841 tgacttattc ccgccaatat gctaaaaata aactataatt tataggatga atattcaccc
    30901 aataaaagga ggagcaggaa catgaaagaa ctatatgatg tgctcgtcat cggcggaggt
    30961 gtagtgggga gcgccgttgc cagagagctg tcacgatatc gtttaaggat cggcgttctg
    31021 gagaaaaatc tggatgtctg caatgagacc agcgggcgca attccggcgt ggttcacggc
    31081 ggctttgctt atgacagagg aagtttaaag gcaaagctct gcgtagaggg aaaccagatg
    31141 atgggagagc tggctaagga actggatttt cgctttatcc gctgtggaaa ggtgctggtc
    31201 ggcaatacgg atgaggacat ggagacctta aagcgcacga tccgccaggg agaggacaat
    31261 ggggcctcag gattagagct cattgacgaa aaacgtctcc acgagctggt acctgcggta
    31321 gtgggaaaat ttgccatgta ttcaaagaac agcgggattg tagatccctt tggctatacc
    31381 attgccctgg cagaaaatgc ccatgcgaac ggggctgagt atcatttcgg ctgtgaggta
    31441 acggatattt ccagggacgg agacggaaat tactgtattt ccacttccgg gggaaccttt
    31501 tacagccgct gggtggtaaa cagtgcaggc cttggatgtg gaaaaatttc cgatctccta
    31561 ggaattacgg gatatcatat catcggctcc aagggcgatt acattattct ggataagcgt
    31621 accggcccgc tgcttcctat gcccgtttat ccggttccaa gcaatacata tatgggaatt
    31681 catgtgacga ataccacaga cggaaatgtt attatcgggc ccaatgcgga tctggtgacg
    31741 gatttcacct actatggagt gccgcaggaa aatatggatt atctggctaa cagcgcttcg
    31801 gatctgtggc cctgtatccg taagcaggat tatatccgca attattccgg tatccttcca
    31861 aaatgggtgg atgatcaggg agtgatccag gattttaaga ttgagatacg ggatgatctt
    31921 gcgcccaggg ccatcaatct ggtaggaata gagtctccgg gactgacagc agcagtaccc
    31981 attgcgcggt atgccattga aatgatgaag gaaagggaaa aactggaaga gaatcctgat
    32041 tttaatccgg aaagaaaggg aatccgccgc tttgaggaat gttccatgga ggaacaggcc
    32101 cgattgattg aagaagatcc agattatgga gaggtgatct gccgctgtga gaaggttacg
    32161 aaggcggaaa ttttaaaggc tatccataat cctctgggag tggataccat gacaggcatc
    32221 aaatacagga cacgttccat gatggggcgc tgtcagggcg gttactgcca gatgcggatc
    32281 gagcagctca tcgaacagga gctggggaaa aaagaaaccc aggtgcagta tgagcgggaa
    32341 ggttcacagg tattgttcgg aaaggttcgg gaggtgtagg atatgggaca gttagagcat
    32401 gagatcgtct gtgaagcagt ggatgcagtc atagtgggcg gcggtccggc aggattggcg
    32461 gcagcagtgc ggctgtatga aaaagggatt acgaatattc tgattctgga gcgggagaaa
    32521 catctgggcg gtatcctgcg ccagtgtatc catgacgggt ttggcctgac ccgctttggc
    32581 ctgaccctaa gcggcccgga atatgcacag aaatttatcg accgggtgca tgaacttaag
    32641 atcccctatc tgacggacgc cacggtttta gaggtgacaa aggaacatgt ggttactgca
    32701 gctacgcctc agggcttaaa gcagtggaag gcaaaggcag tgatccttac tatgggctgc
    32761 agagagcgca cccgcggggc tctggggatt ccgggagaaa ggcccgcagg cgtatttact
    32821 gcaggagtgg ctcaggcata tatcaatctg tataaccgga tgcctgcaaa ggaagtggtg
    32881 attctgggtt caggtgatat cggtatgatt atggcacgac gccttacgtt agagggagcc
    32941 catgtaaagg ctgtatttga gatccagcca tatccaagcg gccttcccag aaatattgag
    33001 cagtgcttaa acgactatgg aatccccctt tatctgagcc atacggttac agccatccac
    33061 ggggataaac gtctgaccgg tgtgacggtt tctcaggtag atgagcatat gaaaccgatt
    33121 cctggaacgg aaaaagagta tgattgcgat acgctgatcc tgtcggtagg cctgattcct
    33181 gaaaatgagc tttccctgga tgccggcgta accttggatg gaagaacgaa gggcgcagaa
    33241 gtagatgagt atttccagac ggataggccc ggtatctttg cagcaggaaa tgtgctccac
    33301 gtccatgatc tggtagactt tgtgtccatg gaggcggaac ggctggcgga tgctgcagcg
    33361 gaatatgtag ataagggatg tctgccggag tgtcccgttg tgattcgtac cgattgtttt
    33421 atcaatcaca cagtgccgca gcgtatttcc ggaaaagagg actgcagatt gtcactgcgt
    33481 gtaaacaggc catttaagga ctgccgtatc gtagtacgtc aggatggaac ggaggtggct
    33541 tttaaaaaga tgaaaaaagc cattccggca gaaatgattc agattccatt aaaggcagag
    33601 aagctgtcag gctgtaaaga actggaggtg agtgtggaat ggtaagagaa tttacctgta
    33661 ttatctgtcc aaatggctgt gagatcacag cagaacttga gaaaacggag gatggagcct
    33721 gccggatccg ttccatagaa ggagcagcct gtccaagagg gaaagcttat gtggagcagg
    33781 agctcaccga tcccagaaga aatatcgcaa cctccatttc ggtagaggga ggagagctgc
    33841 cccttacaag tgtccgcctc acggccccca taccaaaggc aaggatcttt gacgccatgg
    33901 aagaaatccg cagatgccgc gtcactgcac cagtgaaagc agggactgta gtgatagaag
    33961 gacttttagg atacgattcc gatgtgatcg tgacgaagac ggtggagaaa cagaaataaa
    34021 atcatgtata cagacagccg gaccagtggt gaaaacgggt ccggctgtct gtatttaaga
    34081 agggttttta aatgaatgtc cttaacattc tggatgaaat atggtatttt ttaatgaaag
    34141 aacgattaca gaaggaggag aaatgcatga gccttttcat tggaaagaca gaacgggtat
    34201 attccacctc agacagcttc cagaaggaac gggtacagga aattctgaat gaaaatcata
    34261 tagaatacaa gattcgggcc cgggatgatt caagacgaaa tccgtttgat caggccggac
    34321 ttggaagcct gggaaataac aggcttctgg ttacatattc cttttatgtg gataaaaagg
    34381 atgcagagtg ggaagtgagg ttgattcatt cacaggtgta gagcccatct acaaaccttc
    34441 tccatccttg cattttcccc caatctatta tatagtggaa tgcagagaga aaatatacga
    34501 aacaggagga aacgtatgtc gggaaagcgc gaggattata ttacatggga tgaatatttt
    34561 atgggagtgg cctgtctgtc ggggaaacgc tctaaggatc ccagcaccca ggtgggagcc
    34621 tgtatcgtaa gccaggacaa caagatcctt tctatgggct acaatggttt ccccaagggc
    34681 tgttcagatg acgaatttcc ctgggagaag gagcatgagg cggacgatcc ctaccatgca
    34741 aaatactttt attctaccca cagcgaattg aatgccattt taaattacag aggcggaagc
    34801 ctggagggca gcaagcttta tgtgaccctt ttcccctgca atgaatgtgc caaggccatt
    34861 attcaggcgg gaattacaac gattgtctat ggggaagaca agtatgccga tacgcctgcc
    34921 gtgcgggctt ccaagcggat gctcaatgca gccggagtaa ggtattatca gtatcagcct
    34981 acagggcgta aaattgaaat agaggtttag ggaaaatgaa ggttcttctg gcagcggtaa
    35041 atgccaaata tatccattcc aatctgggga tctacagcct gaaggcttac ggtgagaaga
    35101 tgctggcaga gcagggcttt aaaagagaac agatacagat cgagctggcg gagtatacca
    35161 tcaaccacca gttagaacag attcttaaag atctgtactg caggaagccg gatgtgatcg
    35221 gtttttcctg ctatatatgg aatatttctt atataaaatg tcttctggca gatataaaaa
    35281 aggtgctccc ggaagtgaag atatggcttg gaggtcctga ggtatcctac tgcggtgagg
    35341 agattttaag ggaacagcct gtggtggatc tggtgatgaa gggagaaggc gaggaaacct
    35401 ttgcacagct tttacaggtt ctggtggaaa aggagggatc aggagcctcc ttcagtggat
    35461 atctggaaga aataaaacaa aaggggattc ccggactggt atttttggac agtcagggca
    35521 ataaagacgg cgcagcctgg ggcacttttg cgggcggtga tagacaggca ggatggcccg
    35581 tttatgacac cggtatccgt ccgcccatgg atatgaaccg gatccccttt ccctatggat
    35641 ttttggagat ggaacaactg gaacacagga ttatttacta tgagagcagc agaggctgcc
    35701 ctttttcctg tgcttactgc ctgtcgtcca ttgataagag cgtccgtttc cgcagtctgg
    35761 aacgggtaaa ggaagagctg tcctttttct taaatgccag agtaccgcag gtgaagtttg
    35821 tggaccggac ctttaactgt aaaaaatccc acgccatggc agtctggcag tttatccggg
    35881 aaaatgacaa cggcattacg aactttcatt ttgaaatcgc agcagatctt ctggatcagg
    35941 aggagctgaa cctcctttct tccatgcgtc cgggactgat tcagctggaa ataggagtcc
    36001 agtccaccaa tccggacacg ctttcggcaa tccggagaaa aacagatatc gcagaaataa
    36061 gaaagattac ccatcatatc aacagccggc acaacatcca ccagcatctg gatctgatcg
    36121 caggccttcc ccatgaggat ttaagcagct ttagaaaatc ctttaatgag gtatatgata
    36181 tggagccgga acagctgcag ctgggatttt tgaaggtgtt aaaaggctcc tatatggaag
    36241 aaatgattcc tgcctgtgag ctgttatatt catcttcccc gccctatgag gtcttgtcca
    36301 cccgatggct ttcttatgga gatatcctgg agttaaaggc tgtggaagag atggtggaag
    36361 tctactataa cagccgccag tttacctgta cactggagca gctgaggcag gaatttgata
    36421 cgccctatga aatgtttctg gatttggccg gattttacca gaagaaggga tatggcggcg
    36481 taaatcacag ccgtctggca cggtatgaga tcctgtggga gcaggcagag gaaatgtttt
    36541 ctgacggcag cagaacagat gcagagagaa agcgtgagca gtacagagat gctctgatga
    36601 ccgatctgta cctccgggaa aatatcaaaa gccgtccttc ctttgccagg aatctttcac
    36661 atttcaagga gcagatccgg cgttttttca ggcaggagga ggactgcccc tgcctacttc
    36721 cggattatac aggatatgat tcccgccagc tggcaaagat ggcccatctg gagccggcgg
    36781 gggacggaag ttttctgctg tttgactata aaaaccgcga cccgctgacc tataatgcaa
    36841 ggatggttcg ggttgagatt
//
LOCUS       DBMDUH010000006        34722 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_1003_length_34722_cov_4.511944, whole genome shotgun sequence.
ACCESSION   DBMDUH010000006 DBMDUH010000000
VERSION     DBMDUH010000006.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 34722)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 34722)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34722
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1003_length_34722_cov_4.511944"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(348..3329)
                     /locus_tag="ACZPCG_01355"
                     /pseudo
     CDS             complement(348..3329)
                     /locus_tag="ACZPCG_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005426600.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4368 domain-containing protein"
     gene            complement(3326..3802)
                     /locus_tag="ACZPCG_01360"
     CDS             complement(3326..3802)
                     /locus_tag="ACZPCG_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002582191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HIR1283751.1"
                     /translation="MQEEVTQKTIALVIKAAKLDANILKSAMRMYLNHCRKQAQKTHG
                     KVSVKELVGEGAGASSIEITDGNIKSFERVARKYNVDFAVKKDKTTEPPKYLVFFKGR
                     DADAVAQAFKEFVYGNEKKNSRTSVRQKLKHFRDVVSKDQNKERSREKNKDRGQSL"
     gene            complement(3922..4347)
                     /locus_tag="ACZPCG_01365"
     CDS             complement(3922..4347)
                     /locus_tag="ACZPCG_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1283752.1"
                     /translation="MKKREFQKKAAAALLGKLADVVMELEENPNITITSEYHSSYFKL
                     RKNYNLIQISVKQTGELTYQLTDMLGNTGRSQIYVQYHAGDDPEHLIKKFVEDFGRLY
                     KDFLLAGTLHDFVRIQLSVDADTEEALREYEDCIRAGCL"
     gene            complement(4462..5010)
                     /locus_tag="ACZPCG_01370"
     CDS             complement(4462..5010)
                     /locus_tag="ACZPCG_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004070568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1283753.1"
                     /translation="MKPNHHSLAYKQQKQPNKTYKDLKQKQKMKIADWMFRETCIFYK
                     ENGEIPNEEVAKQIIDRIYEKLKSLAIWVPYEEVYRAYLLKLPRYELRIAENGIPEEK
                     PPKEKKEDVPKKKKGSSNKRCPVCGRRMKQQFIGLQHCKCGMSWKKDIGFFERTGDMV
                     FALERRKIGNKQKQCPVIRYKE"
     gene            complement(5089..5745)
                     /locus_tag="ACZPCG_01375"
     CDS             complement(5089..5745)
                     /locus_tag="ACZPCG_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1283754.1"
                     /translation="MESLRDVKRVMEREIEKGSCPLQFEYLEFGEKPFQFIESEEKLN
                     EVLSYLLRLGEYRGYTEHLVGNNVYMDLDMLCRKIQFKRTHSPVERSEIQWKIQRYKN
                     KLKPDYAGKVCLEIVQCVFSLPEGAAEKYQIVYDGQKTYAFAMSNKYILGLFTHCEAA
                     RKSAVEDGVEFVHLPENEQKIVSLENVRNVLFQALLLDNVTVENGLFHAQMCTVMLLK
                     "
     gene            complement(5838..6206)
                     /locus_tag="ACZPCG_01380"
     CDS             complement(5838..6206)
                     /locus_tag="ACZPCG_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002596307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1283755.1"
                     /translation="MEKSYITDIERERCKKVAAAYAELYELESILVLDVGRYGFVKLQ
                     YYTPEYGFNDVITYTDSESMFEDLWQEWLDTRLYLFAKGTPMLEMGYEEIFKCLPEEK
                     QKELLEQKAVFAKMAEIELK"
     gene            complement(6245..6406)
                     /locus_tag="ACZPCG_01385"
     CDS             complement(6245..6406)
                     /locus_tag="ACZPCG_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1283756.1"
                     /translation="MEHNEFKDQLYEVLDENDVALGIEDIDTSDAANIFTIKTRDGSV
                     FEIETRKIE"
     gene            6506..6712
                     /locus_tag="ACZPCG_01390"
     CDS             6506..6712
                     /locus_tag="ACZPCG_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016220459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1283757.1"
                     /translation="MIKYDPLWRTLKEKGISQYQLIKDYGIDKAQLQRLRQNLVVKTL
                     ILNRLCQILNCRIEEIMEYVPDDN"
     gene            complement(6759..7091)
                     /locus_tag="ACZPCG_01395"
     CDS             complement(6759..7091)
                     /locus_tag="ACZPCG_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6050 family protein"
                     /protein_id="HIR1283758.1"
                     /translation="MKRILVKIVLPVSLLAVWMITCYPICRKAEGFDFFLYWVLVGCP
                     YGIRKMCMILIPKNFGIAGSIGILALNCIVGGLIGGVVVILRMVMVLMEIIKIISDTS
                     GHDVQRCR"
     gene            complement(7072..8136)
                     /locus_tag="ACZPCG_01400"
     CDS             complement(7072..8136)
                     /locus_tag="ACZPCG_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016288131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6017 domain-containing protein"
                     /protein_id="HIR1283759.1"
                     /translation="MGKQIQFDYYYGIEAEQFSFYRVPRLLIKDERFKGLSSDAKLLY
                     GLMLDRMSLSMKNGWLDEENRAYIIYTLDNVMEDLGCAKATCVKIMKELDSHNGIGLI
                     EKKRRGLGKPDIIYVKNFATIGEAEEETPANADVSTEVQDLNLKSSKSYTSRSSETEL
                     QEVQKADFQKSKNFTSRSTISELAEVQKADPNYTNYNHTNQSNIDRNYINPINQSGSE
                     LEGEIDVMEDVNAYMEIIKENIEYEHHMKYGRWQDKGLYEELYEVICEIVCVKHKTVK
                     IGGNDYPYELVKSKFLKLNSSHLEYVIGCMQETTTKIANIKAYMVTALYNAPSTMNHY
                     YQQEVQHDMYGGGWNEKNIG"
     gene            complement(8152..9033)
                     /locus_tag="ACZPCG_01405"
     CDS             complement(8152..9033)
                     /locus_tag="ACZPCG_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002596302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIR1283760.1"
                     /translation="MSKAGSAAKVKLSSYDDLFGNAEEKTGSEEQIIRAKLTDLHEFR
                     NHPFRVLDDEKMEETTESISKYGVLVPGIARPRAEGGYEIIAGHRRKRGSERAGKEDM
                     PVIVRNYTDDEATIIMVDSNIQREDILPSEKAKAYAMKYEAMKHQGRKGNGNTLDEVG
                     EAAGESGKTVQRYIWLARLSDELLELVDRKKIGLVQGVDISFLSEDAQQWVLVILEES
                     GANISTVQSAKLKEYGKSGELTLPMVRLILTEEKPKERKVTIKSEKISQFFSEEYSSE
                     EIEGIILQLLEEWQKKQ"
     gene            complement(9026..9808)
                     /locus_tag="ACZPCG_01410"
     CDS             complement(9026..9808)
                     /locus_tag="ACZPCG_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009221111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR1283761.1"
                     /translation="MCKIIAIANQKGGVGKTTTTSNLGIGLAKQGKKVLLIDADAQGS
                     LTDSLGFTEPDKLEETLATALGNIINDEDMEADYGILKHKEGIDLMPGNIELSGLEVS
                     LVNVMSRELVMRSYIELVKDNYDYILIDCMPSLGMITINAFACADSILIPVQAAYLPA
                     KGLQQLIKTIGKVRRQINPKLMIEGILLTMVDARTNYAKDITALLVENYGSKVRIFEN
                     SIPISVRAAEISAEGVSIYEHDPKGKVAAAYQSLTEEVLGNE"
     gene            complement(9832..10008)
                     /locus_tag="ACZPCG_01415"
     CDS             complement(9832..10008)
                     /locus_tag="ACZPCG_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008754293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1283762.1"
                     /translation="MLKVRLMGTKNDIKWFGKILQRNPKVEVTEFSEMYPNKGTKKFY
                     RAYVEVKKRNVAEK"
     gene            complement(10008..10481)
                     /locus_tag="ACZPCG_01420"
     CDS             complement(10008..10481)
                     /locus_tag="ACZPCG_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6075 family protein"
                     /protein_id="HIR1283763.1"
                     /translation="MTSTALGAETKKEEKIIFISDAHEKFYYEKLKEVRYQDVYHKAL
                     CYCLGISDDTRRNVYRIYDFKTGCVITECLHEGWQTSGSVRVIRMAFNLYCNGTPSVS
                     DYEDAEEQVDECRQYTVEEIFCCAYAPYFWQAIQIRYPEYATYNHKLYALLGGTD"
     gene            complement(10720..12660)
                     /gene="lysS"
                     /locus_tag="ACZPCG_01425"
     CDS             complement(10720..12660)
                     /gene="lysS"
                     /locus_tag="ACZPCG_01425"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002596298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HIR1283764.1"
                     /translation="MGEQKSNQTEQDLNHVLKARRDKLAELQAAGKDPFQITKYDVTA
                     HSMEIKDHYDQWEEREVSIAGRMMFKRVMGKASFCNLQDLQGRIQVYVARDSVGEDAY
                     KEFKKLDIGDIVGVKGTVFTTKTGEISVHASEFTLLSKSLQVLPEKFHGLTDTDLRYR
                     QRYVDLIMNEDVKDTFIKRSKILAAIRKYLGGEGFMEVETPMLVSNAGGAAARPFETH
                     FNALDEDLKLRISLELYLKRLIVGGLERVYEIGRVFRNEGLDTRHNPEFTLMELYQAY
                     TDYHGMMDLTENLYRFVAQEVLGTTKIVYNGVEMDLGKPFERITMLDAVKKYSGVDFN
                     EIHTLEEARAAAKEKHVEFEERHKKGDILNLFFEAFVEEHLIQPTFVMDHPVEISPLT
                     KKKPENPEYTERFEFFMNGWEMANAYSELNDPIDQRERFKAQEEQFAAGDEEANHTDE
                     DFLNALEIGMPPTGGIGFGIDRMCMLLTDSAAIRDVLLFPTMKSLDAKKGEGKAEKAV
                     ENAAVAEEKVAEKIDFSNVKIEPLFEEMIDFDTFAKADFRAVKILECEAVPKSKKLLK
                     FTLDDGTDRKRTILSGIHEYYEPEDLIGKTAIAIVNLPPRKMMGIDSEGMLISAVHEE
                     DGHEGLNLLMVNDRIPAGAKLY"
     gene            complement(12705..13187)
                     /gene="greA"
                     /locus_tag="ACZPCG_01430"
     CDS             complement(12705..13187)
                     /gene="greA"
                     /locus_tag="ACZPCG_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008716565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HIR1283765.1"
                     /translation="MADKKHILTYAGLKQYEDELQNLKVVKRKEVAQKIKEAREQGDL
                     SENAEYDAAKDEQRDIELRIEELEKLLKNAEVVVEDEIDVDKINIGCKVKLLDVEYDE
                     EMEFFIVGSTEANSLQNKISNEAPVGHALMGKSVGDVVDVETQAGVIQYKVLEIQRVS
                     "
     gene            complement(13404..14366)
                     /gene="dusB"
                     /locus_tag="ACZPCG_01435"
     CDS             complement(13404..14366)
                     /gene="dusB"
                     /locus_tag="ACZPCG_01435"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003501903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HIR1283766.1"
                     /translation="MKLRLGDRELNSNIILAPMAGVTDLPFRLLCHEQGAGMAVTEMV
                     SAKAILYKNKNTKELMAVDPAEGPVAVQLFGSDPEIMAEIAAQIEDGPFAAIDVNMGC
                     PVPKIVNNGEGSALMKDIKLAEKILTSMVKAVKKPVTVKFRKGFNDQCLNAVEFAKMA
                     ESSGAAWVAVHGRTREQYYSGTADWEVIRQVKEAVKIPVIGNGDIFHAQDAARMMQET
                     GCDGVMVARGAKGNPWIFREIRQYLETGEIPPRPSTEEIREMILRHGRMLSEYKGENV
                     AMREMRSHMAWYTKGMKHSASLRCEINQVETLEGLAALLDGRMV"
     gene            complement(14356..14718)
                     /locus_tag="ACZPCG_01440"
     CDS             complement(14356..14718)
                     /locus_tag="ACZPCG_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6145 family protein"
                     /protein_id="HIR1283767.1"
                     /translation="MMDKVVLCGASSYEQKYYFNEEFKSLPQSVQDELHIMCVLFTED
                     VGGVLTLEFDDRGRLEFKVVSEEGDYLFDEIGSVLKIKQYQEEKKDLLEALELYYRTF
                     FLEEDVDWEESGRDEDEA"
     gene            complement(14779..15753)
                     /gene="pfkA"
                     /locus_tag="ACZPCG_01445"
     CDS             complement(14779..15753)
                     /gene="pfkA"
                     /locus_tag="ACZPCG_01445"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR1283768.1"
                     /translation="MAKAVKTIGVLTSGGDAPGMNAAIRAVVRVAINKGLKVKGIMRG
                     YAGLLEEEIVDMESTSVSDTINRGGTILYTARCKEFTTPEGQQRGAEICRKHGIDGMV
                     VIGGDGSFKGAGKLSALGINTIGLPGTIDLDIACTDYTIGFDTAVNTAMEAIDKIRDT
                     STSHERCSIVEVMGRNAGYIALWCGIANGAEDILLPERYDGDEQALINRIIENRKRGK
                     KHNIIINAEGIGHSSSMAKRIEAATGIETRATILGHMQRGGTPTCKDRVYASIMGARA
                     AELLVEGKSNRLVAYRKGEFVDFDIQEALNMKKDISEEQFNIAKMLVR"
     gene            complement(15832..19317)
                     /locus_tag="ACZPCG_01450"
     CDS             complement(15832..19317)
                     /locus_tag="ACZPCG_01450"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HIR1283769.1"
                     /translation="MAFTHLHVHTEYSLLDGSSKIKELTARAKELGMDSLAITDHGVM
                     YGVIDFYRAAKEVGIKPILGCEVYVAPGSRFDRENTGGEDRYYHLVLLAENDQGYKNL
                     MKIVSKGFVEGFYYKPRVDYEVLETYHEGVIALSACLAGEVQKYLARGMYEEAMRSAQ
                     RYEGIFGKNNFFLELQDHGLPEQKIVNQGLLRLSRDTGIELVATNDIHYTFAEDEKAH
                     DILLCIQTGKKVADEDRMRYAGGQYYCKSEEEMQALFPYAREALENTHKVAERCNVEI
                     EFGVTKLPKYEVPEGFDSWTYLNHLCREGFKTRYPKDDGTLSRRLDYELDVIRTMGYV
                     DYFLIVWDFINYARSKDIMVGPGRGSAAGSIVSYTLGITNIDPVRYNLLFERFLNPER
                     VSMPDIDVDFCYERRQEVIDYVVEKYGKDQVVQIVTFGTLAAKGVVRDVGRVLDLPYA
                     MCDSIAKMIPNDLGMTLDKAIAANPDLRKLYNEDAQVKYLIDMSKRLEGLPRHTSMHA
                     AGVVIGSRSIDEFVPLSKAADGTITTQFTMTTIEELGLLKMDFLGLRTLTVIQNAVHL
                     AEKDYGISIDIDHIDFDDKRVLESIGTGRTEGVFQLESGGMKSFMKELKPENLEDIIA
                     GISLYRPGPMDFIPRYLKGKNDKSSITYECPQLEPILSPTYGCIVYQEQVMQIVRDLA
                     GYTMGRSDLVRRAMSKKKTAVMEKERQNFVYGNEAEGVKGCIANGIDEKTANHIYDEM
                     IDFAKYAFNKSHAAAYAVVSYQTAYLKYYYPKEFMAALMSSVMENVSKFSEYILNCRR
                     MMNIAVLPPDINEGESGFSVSGGGIRYGLSAIKSVGKPVVDAILEERAKNGKFRTMED
                     FINRMTQREVNRRTLENFIKSGALDSLPGTRRQKMAVGPALLENKARERKNAFEGQLS
                     LFDIAGEEEKKEFEVVFPDVGEYAKEELLAFEKDILGVYISGHPLDDYETLWRKNITA
                     TAADFIVDEETEEAVVKDGIKAVIGGLVTGKTVKTTRTGQLMAFITLEDLMGSVEVIV
                     FPRDYENNRDLLTEDEKLFIRGRVSLGDEPVGKLVCEQVIPFDAVPRQLWLQFEDMAG
                     YQAAEAELMELLKSSEGNDQVVIYLKKERAKKKLPPNWNVMADAGLLNRLYCKVGEKN
                     VKVVEMALDKSWKMN"
     gene            complement(19433..19687)
                     /locus_tag="ACZPCG_01455"
     CDS             complement(19433..19687)
                     /locus_tag="ACZPCG_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HIR1283770.1"
                     /translation="MKKKIITVELPDGLEARPAAMLVQVASQYDSRIYLESGSKRVNA
                     KSIMGMMTLGLIAGQSITVEADGADEDQAVSEIEKYLINA"
     gene            complement(19724..20683)
                     /gene="whiA"
                     /locus_tag="ACZPCG_01460"
     CDS             complement(19724..20683)
                     /gene="whiA"
                     /locus_tag="ACZPCG_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007713173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HIR1283771.1"
                     /translation="MSFSSKVKEELSRQWSPARHCQIAETAAVLSLCGRVKISASDHY
                     AIEIHTENLAVARKYFTLLKKTFNINTDVLIRRGQMPGRSRTYMVAVREHEDALRVLQ
                     ASKLLDSYGEIAEDLSLIHNVVVQNVCCKRAFIRGAFLSAGSISDPEKFYHFEITCAS
                     MRKAKQLQGLMASFDIEARIVLRKRYFVVYVKEGSQIAELLQIMEAPVALMELENIRI
                     VKEMRNSVNRQVNCETANINKTVSAAVKQMEDIRYIQDTIGLESLPENLQEMARVRLE
                     RPEATLKELGEALEPPVGKSGVNHRLRKLSLMAEDLREKFPEQ"
     gene            complement(20706..21572)
                     /gene="rapZ"
                     /locus_tag="ACZPCG_01465"
     CDS             complement(20706..21572)
                     /gene="rapZ"
                     /locus_tag="ACZPCG_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HIR1283772.1"
                     /translation="MKLVIVTGMSGAGKTIALKMLEDLGFYCVDNLPISLADKFVQLV
                     SDGGTVQKAALGIDIRSGEGLKDLEGVIEKWRREQIDVQIIFLDASNTALIKRYKETR
                     RSHPLAGKGRLETGIEKERARVAFLKKESDFIIDTSMLLTKELRQELEKIFLKEESFK
                     NMFVTVMSFGFKYGIPEDADLVFDVRFLPNPYYVEELRPLTGENQSVRDYVMQGNTAE
                     TFIEKLNDMMDFLLPNYINEGKNQLVIAVGCTGGKHRSVTIAKALYDHLKSHGEYGIR
                     MDHRDIDKDSKR"
     gene            complement(21610..22521)
                     /gene="murB"
                     /locus_tag="ACZPCG_01470"
     CDS             complement(21610..22521)
                     /gene="murB"
                     /locus_tag="ACZPCG_01470"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HIR1283773.1"
                     /translation="MELKEKLQALLELEGFPCRFMEPMSSHTTFRIGGPAAAYVCPSG
                     ETELKTVLSFCRREKLPFFILGNGSNLLVSDAGYEGVVISTTEGLTRLEAEGERIFAS
                     SGQLLSRTAKRALKEGLTGLEFAAGIPGTIGGALVMNAGAYGSEIKDVLEYARVMTPE
                     GEILTLGPKELKMDYRTSCIPAMGYTVLDACFRLKKGNRDEIEAYMNELSLKRREKQP
                     LQYPSAGSTFKRPKGHFAARLIEDAGLKGFTVGGAQVSQKHSGFVINRGDATAADVME
                     LCRQIRERVKTQFDVELEMEVKTLGKF"
     gene            complement(22547..23479)
                     /gene="hprK"
                     /locus_tag="ACZPCG_01475"
     CDS             complement(22547..23479)
                     /gene="hprK"
                     /locus_tag="ACZPCG_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HIR1283774.1"
                     /translation="MHGVTISELIEKMKLRNTTPEIDTDKIVLTHPDVNRPALQLTGF
                     FDHFDRERVQIIGYVEQAYINTMDREVKRQMYDKLTSSQIPCLVFSRGQNPDDDLLEY
                     CNYYGVPCLVSDKTTSSLMAEVIRWLNVKLAPCISIHGVLLDVFGEGVLIMGESGIGK
                     SEAALELLKRGHRLVTDDVVEIRRVSDDTLIGSAPDITRHFIELRGIGIIDVKTLYGV
                     ESVKDTQSIDMVIKLEDWNRDKEYDRLGLEDQYTEFLGNQVVCHTIPIRPGRNLAIIV
                     ESAAVNHRQKKMGYNAAQELYNRVQANLKNGMND"
     gene            complement(23561..25483)
                     /gene="uvrC"
                     /locus_tag="ACZPCG_01480"
     CDS             complement(23561..25483)
                     /gene="uvrC"
                     /locus_tag="ACZPCG_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HIR1283775.1"
                     /translation="MFDLEEELKKLPASPGVYLMHNDRDEIIYVGKAVSLKNRVRQYF
                     QSSRNKTAKIEQMVSHIAWFEYILTDSELEALVLECNLIKEHRPRYNTMLKDDKTYPY
                     IKATVGEDFPRLLFSRDMKKDGRSRYFGPYTSAGAVKDTLDLIHKLYRIRTCSRNLPK
                     DTGKERPCLNYHIKQCDAPCQGYISKEDYQKSFGQALDFLGGHYEPLLRSLKEKMEQA
                     SEQMEFEKAIEYRELLNSVKQVAQKQKITSSSMEDRDIVALARDERDAVVQVFFIREG
                     KLIGRDHFHLSAGAAESEEEILDGFVKQFYAGTPFIPRELWLQYPITDSEVISQWLTA
                     KRGQKVRLVVPQKGEKERLVELAARNAALVLSQDKERIKKEELRTIGAMNQIGALIGI
                     GAVRRVEAFDISNTSGVESVGSMVVYEDGKPKRSDYRKFKIRTVQGPNDYASMEEVLT
                     RRFSHGMEEAKNLRAEGKELSFGSFTRFPDLIMMDGGRGQVNIALGVLEQMGLSIPVC
                     GMVKDDFHRTRGLYFNNKEVPIDRHSEGFRLITRIQDEAHRFAIEYHRSLRGKGQVRS
                     VLDEIPGIGPARRKALMRQFKDIEAIRNADIQELAQTPQMNQKAAEAVYHFFRKTGGH
                     SFCGGMCADVKTHDNQ"
     gene            complement(25498..26010)
                     /locus_tag="ACZPCG_01485"
     CDS             complement(25498..26010)
                     /locus_tag="ACZPCG_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002600627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HIR1283776.1"
                     /translation="MPVPGAFYRHFKNKLYQVIAVAVHSETGEPMVVYQALYGTFGMY
                     VRPLEMFMSPVDREKYPDADQEYRFERVWPENDPVRTAWQEREQGETDKEEELPGPWL
                     ERFLDALRPEDKLAVLKQMEGKATKRELDCMFMAMDLKPEPEMPVEEQIQALRRYVQL
                     LRRYDGTRLR"
     gene            complement(26211..26360)
                     /locus_tag="ACZPCG_01490"
     CDS             complement(26211..26360)
                     /locus_tag="ACZPCG_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1283777.1"
                     /translation="MKLKKGLECFAWVILAAVLILVNAAAANADEFSGQEYLAAGKDA
                     AASLQ"
     gene            26531..26746
                     /locus_tag="ACZPCG_01495"
     CDS             26531..26746
                     /locus_tag="ACZPCG_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1283778.1"
                     /translation="MITYKPFWETLKKSPETTYTLIHKHQISSATIDRLRKDKPLSTI
                     TIDDLCHILNCQVGEIAAYIPPEDKPH"
     gene            26837..27709
                     /locus_tag="ACZPCG_01500"
     CDS             26837..27709
                     /locus_tag="ACZPCG_01500"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HIR1283779.1"
                     /translation="MQKIGRNDPCWCGSGKKYKDCHMELEEKLTQFRRQGAIVPTRAL
                     LKTPEQIRGIKESARINVAVLDYVAAHIGPGITTAQIDLWVYEQTTRLGGIPAPLGYN
                     GFPRSVCTSVNDQVCHGIPSENDILKEGDIINIDVSTIYNGYFSDSSRMFCIGAVSPE
                     KKRLVDTAKQCLEIGLTEVRPWGFLGDMGQAIHNFAKENGCSIVREIGGHGVGLEFHE
                     EPWVSYVSKKGTEMLMVPGMCFTIEPMVNMGRPDVFVDANNDWTVYTDDGLPSAQWEI
                     QVLITEDGYEILAW"
     gene            complement(27787..27859)
                     /locus_tag="ACZPCG_01505"
     tRNA            complement(27787..27859)
                     /locus_tag="ACZPCG_01505"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27824..27826),aa:Ala,seq:ggc)
     gene            complement(28033..31569)
                     /gene="nifJ"
                     /locus_tag="ACZPCG_01510"
     CDS             complement(28033..31569)
                     /gene="nifJ"
                     /locus_tag="ACZPCG_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HIR1283780.1"
                     /translation="MARKMKTMDGNHAAAHASYAFTDVAAIYPITPSSPMAEATDEWA
                     TDGRTNIFGHEVQITEMQSEAGAAGAVHGSLAAGALTTTYTASQGLLLMIPNLYKIAG
                     EQLPGVFNVSARALASHALSIFGDHSDVYACRQTGCAMLCESSVQEVMDLTPVAHLAA
                     IKGKVPFINFFDGFRTSHEIQKIETWDYEDLKDMADMDAIAAFRKNALNPNHPCQRGS
                     AQNPDIFFQAREACNPYYDALPAIVQEYMDKVNAKIGTDYKLFNYYGAADAEHVIVAM
                     GSVNDTIEETIDYLVAQGKKVGVVKVRLYRPFCAQALIDTIPESVKTITVLDRTKEPG
                     AMGEPLYLDVVAALKGSKFDQIKIQTGRYGLGSKDTTPAQIVAVFENETKSPFTIGIV
                     DDVTNLSLEIGAPLVTTPEGTTNCKFWGLGADGTVGANKNSIKIIGDNTDMYAQAYFD
                     YDSKKSGGVTMSHLRFGHSRIKSTYLIRQANFVACHNPSYVRKYNMVQELVDGGTFLL
                     NCPWDMEGLEKHLPGQVKKFIADHKINFYTIDGVKIGIETGMGPTRINTILQSAFFEL
                     TGIIPAEKANELMKAAAKATYGRKGEDVVMKNWAAIDAGAKGVHKVEVPASWSQCEDE
                     GLDYKVVTEGRKEVVDFVNNIQTKVSAQEGNSLPVSAFKDYVDGTTPSGSSAYEKRGI
                     AVNVPVWNVDNCIQCNFCSYVCPHAVIRPVAMTADEAANAPADMKMKDMTGMPGYKFA
                     MTISALDCTGCGSCANVCPGMKGNKALEMKPLEESLSEQAIFDFGQTVSIKDEVVAKF
                     KENTVKGSQFKQPLLEFSGACAGCGETPYAKLITQLFGDRMYIANATGCSSIWGNSSP
                     STPYTMNAKGQGPAWDNSLFEDNAEFGYGMLLAQNAIRDGLKAKVESVMANENATDDI
                     KAACKEWLDTYGIGALNGSATDRLVAALSGVDCPVCKDIVANKDFLAKKSQWIFGGDG
                     WAYDIGFGGVDHVLASGKDINIMVYDTEVYSNTGGQASKATKTGAVAQFAAGGKETKK
                     KDLAGIAMSYGYVYVAQICMGGDMQQTVKALAEAEAYPGPSLIIAYAPCINHGIKKGM
                     NKAQTEEKLALECGYWNNFRYNPAAEKKFTLDSKAPKMEGYQDFLKGEVRYLSLTMKN
                     PERAAELFARNEQEAKERFEHLSKLVALYGNE"
     gene            complement(31856..33676)
                     /locus_tag="ACZPCG_01515"
     CDS             complement(31856..33676)
                     /locus_tag="ACZPCG_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HIR1283781.1"
                     /translation="MNQLENNSRNRSASPGRSRAKGADRGRSASGRASSRGTSSRNTG
                     HGGSRSSSSYRSGQAGRMNAMQNVRRNSRRHRRKGPDYKWIAIGGVILIVVITCIAMA
                     LNGKNSDETGVQATTEAVTETEMKKEVSVDGISITGLSKSQAREVLLKEFPWSMTVSY
                     DSEVYEVNDLMAGKIDSLLNEIYSGEPQESYTLDTAGLEEQIKAEAAACAAKWDKKAK
                     NGSIDSFNAETGKFVFAGEENGFAIDQEKLIDDVTQALAAKDFDAKIEASGSTVAPEI
                     TAASAKEQYKTIGSFTTNTTSNKNRNTNVRLAAEAINGTVIKPGHEFSFNETVGQRTE
                     AKGYKGAAAYNNGEVVQEIGGGVCQVSTTLYNAVFKAGLKISYRRSHTFEPNYVTPGR
                     DATVSYEQPDFKFINTSSTAIGLRASYADQKMTVSVYGIPILEDGITWDLESKKVEDL
                     GVPEPEYVEDQTLDPGVEKTTSKGSSGSRWETYKVVYKDGKEISRELDHKTTYKGHKP
                     VVHRNTSGVVLNPEETTTQATTVTPTVDGMPEGYTGPAETTQSQPGSGTASSAPAEGP
                     GSASTEPSSVPVAPAPSEDAPSGSQGGPGAGAVVVPVKPE"
     gene            complement(33847..>34722)
                     /locus_tag="ACZPCG_01520"
     CDS             complement(33847..>34722)
                     /locus_tag="ACZPCG_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HIR1283782.1"
                     /translation="PELEIQVRDQEGRKLKNMPAPGKRDEEDKAARAYEEFKAMKKQL
                     KTVAASQKQRLELALSSGRMWKKEDWVELFTKKPVMHPFAIGLIWGIYKEETLTGSFR
                     YMEDGSFNTVDEEELELPEDAVIGLIHPIELSKEERDAWKEQLEDYEIIQPVEQLDRT
                     VYLISEEEVGQKAVERFEGVILNDLSLIGKMQPLGWYTGSVQDAGCFYTFYREDGKLG
                     AELHFSGTYIGGQNEEVTIEDVRFYLAGTIERGSYVYDEADDKKALLLEQVPPRYFSE
                     IILQLSKVTASKKEQ"
BASE COUNT         8328 a         9414 c         6736 g        10244 t
ORIGIN      
        1 ttcattttcg gatatagaag aagtagtgga atcagcagct aatgcagttg tagataagga
       61 aaatgtcatt gccaatacga gaatgagaga taatattttt ttcattataa agacctcatt
      121 ctattaattg attaaaaaat aaacagcaaa tctctatatc catcggaatc accactttcg
      181 taatttagag cgcacattct ttgtacatat tgattatatc atatttactt taataaattc
      241 aagttggcaa aatagacaaa ttatactgtt tcggtttagc aacacaaaac acatatcaat
      301 cataatattt gggatgagat aatcttcttt tttagtatag ggtagtttca tgtggtctta
      361 gctcctttct gtggctctct ctgcggtaaa ctgctcaccg ggataaatac gccctttgcg
      421 atatcctcgg tgcggatagc gtcttttctg gcttcgattt cagccttttt cttggctttg
      481 gtgcgttcct catattcttt ctgcttgccg ttagctttac gcttcaaata gttctgatga
      541 agcctgtcct tgcgctcctc tttcttacgg atttcctcta attcctctga tgtcagctcc
      601 acttctccaa atgccggagg aacaaaacgc ccaacaaaat tgaaataaat ctcaacctcc
      661 tgtgttgtgt ctcggctgcc tttgcggtca cgctcatgga caaggatttt ctcaacaaat
      721 tcattgagca tggtaacggt caacgtgtca aaattctcgt atttatcaat cagagcgata
      781 aatcggtcag cgtccttttc atgcttttca tagcccttaa ccgccttttc cagagtagaa
      841 atttcagcgg tcagctcact ttgctccttt gcatattgag cgtccagcat ttcatatcgg
      901 ctgtccggca gtctccccaa agcattgtcc tcataaatct tacagagcag cacttccagc
      961 tcagaaactc tctgcttggc ggtggcaagg cgtgtccgtt gttttctgac ttctgtggtc
     1021 tgctggctgg actgtgcttt ctgtaccacc cggacaaact cggctcggtc atgcttggca
     1081 tattcggcaa tggctttgag catatcagag ataagggaga gtaccacatc ctcattgata
     1141 cggtgctgtg tggtgcagag ctttccgact gggactttgc tgtattggga acaagtatat
     1201 tgagaaatac gcttgccgtt gttagtacgg tggacataca tcttgccacc gcagtcggca
     1261 caataaagca agccagtgag gggagcagct tcgccccagc cgttagggta gcgtctgaca
     1321 tttcctcgga ttttctgcac aaggtcaaaa gttggctggt cgatgattgg ctcatgggta
     1381 ttttcaaaaa tcgtccattc gtcctccggc acataatggc ttttcttgtc cttaaaatgc
     1441 ttgcgtgttt tgaaattgac tgtatgcccc agatactcac gctttttgag gatttctaca
     1501 acggaagaag ctccccagcc gtacacatct ttgaaagtct tgtttttgtt cacaccctct
     1561 ccaaactgtg caaggtaagc agatggtatc aagatttttt cctctgacag cttctttgca
     1621 atctgatacg gtccaaaacc gtcaatcgtc atggcaaaga tacgctttac cacctcggct
     1681 gcttctggat caactaacca ctgagttctg ttttcattcc agaggtagcc gtaaatgact
     1741 gtacctgtga ggtgcttgcc ggacttgcct ttggactgga aagtggaacg gattttgcgg
     1801 ctggtatctc tggcataata ctcattcata atattgcgga acggggtaaa atcatcgtcc
     1861 cctctggcac tgtccacacc gtcattgatg gcaattaagc ggacaccacg ctgacgcagt
     1921 atctccataa tctgaccgac tttcagatag tcacgcccca gtcggctcat gtccttgata
     1981 caaaggtatt ccacatttcc agcttccact tctttcatca ttgccagaaa tccgggacgg
     2041 tcaaagcaag taccactgat accgtcatca gtaaaatgga ctgtgttaga aaagccctga
     2101 tggtcagcat attcttcaag cagcttcttc tggttcgata tgctgttgct ctcgccttgc
     2161 tggtcatcgt ctcggctcaa tcgctcataa agagcagtga ttttttcgtt tcttctcatg
     2221 gcttacgctc ctttcttcga ttttaggttg tgtgttctaa gagacttccc aaccgcttga
     2281 ttaagaagtc ttttagagca tacaacacct gccgcaagct tatatttctt atactgcctt
     2341 acagcaaaat ggttcgggtc tttttcctcc agctcctcaa agagcagatc aacagcattt
     2401 ttgaagtttt catcgtcctc tcttgcttca cttgcatcaa tcatatcaat cagcatggcg
     2461 aaattctgtt cttcctccgg tgcttcgtac cagatgtatg caatcaatgc tgtataatag
     2521 agtttttctg ctttgaccca aaagtcctca ccggattgct gcccctctcc ttttgtgttg
     2581 actatgattg tgttgacgag tttcaaaatg tccttttcgc tccgcagata agcaaatgga
     2641 ttataatgca tggattttgc aaagtttatg gtgttgagga ctttaatctt atacttgccc
     2701 cgcttcagca tcttcccaca ttccactaag attgtgcctt tcgggtcagt gacgcaatag
     2761 gacgagtgca tctgcatcag gttcggcttc acgaaaaatc ttgttttacc ggagccggaa
     2821 ccgccaatca caagaatatt tttatttctt gcatacttcg gttctttcgg tctgccggac
     2881 atcatcagac gctccgtagc ggtcagaaga atattatttt caaatacaga atccatatat
     2941 ggctctatat ctttggcatt cccccaccgt gcagaaccgt attccacacc ctgccggaat
     3001 ttcttggcat tcttcgcctt ataataaaca atgaatttta aggcaacgcc tccggcgatt
     3061 ccaatcagca aatcactcag aaaaaaactg gggaacggat tggaaaacat cctgtccatt
     3121 tggctcatta ctaccatcat cttgtcagac gcattactgc cctctgccac tctccataaa
     3181 aatgctgcct tattacagaa gtacccggca agcacatagg gaagattcag cagtatcagc
     3241 tttttaacat ctgcactttt tgctttttcc ttcagctttt tggggacagc atttaaatct
     3301 ttggtaatgc ctgctatgat attagtcata ggctctgccc cctgtcctta ttcttttccc
     3361 ggctccgttc tttgttctga tcctttgata ccacatcccg gaaatgcttt aatttctgcc
     3421 ttacagaagt cctactgttc tttttctcat ttccataaac aaattcctta aatgcctgtg
     3481 ccacagcatc ggcatccctg cccttgaaaa ataccagata cttcggtggc tcggtggtct
     3541 tatccttttt cacagcaaaa tcaacattgt atttacgagc tacccgctca aaggatttga
     3601 tattgccatc tgtgatttca atggatgaag cacctgcacc ctcgcccacc agttccttaa
     3661 ccgacacttt cccatgcgtt ttctgtgcct gttttctgca atgattcaga tacattctca
     3721 tggcagattt caggatattg gcatccagct ttgcagcctt aatcacaagg gcgattgttt
     3781 tctgcgttac ttcttcctgc atctgtacct ccttctttcc tttttactgc tgttccttca
     3841 gggcggaaca agcagccgat gtagtaaata tttcatgttc tctcctttcc gcttctggac
     3901 atcgtgtcca aaaattctga tttacaggca gcctgctctg atacagtcct cgtactccct
     3961 taatgcttcc tctgtatctg catccacaga taactggata cgaacaaaat catgcagcgt
     4021 tcccgctaaa agaaaatcct tataaagccg cccaaaatcc tcaacaaact tctttatcaa
     4081 atgttccgga tcatctccag catgatattg tacataaatc tgacttctgc ctgtgtttcc
     4141 cagcatatcc gtcaactgat acgtcagctc tcctgtctgc ttgaccgaaa tctgaatcag
     4201 attgtaattc tttctcaatt taaaatagct actatgatac tccgacgtta ttgtgatgtt
     4261 tggattttct tccagttcca tcacaacatc tgccagtttt cccaataacg ctgcggctgc
     4321 ttttttctga aattctctct ttttcatttc atctgacctc catccggctt ctggacatcg
     4381 tgtccaaaag caataaaaaa gggcagctac aaatctttcg actcataact gccctgacgc
     4441 tgttttctgt ttttccatgc cctattcctt atatcgaatg acagggcact gtttttgttt
     4501 atttcctatt tttctccgct ccaaggcaaa caccatatct ccggttcgct caaaaaagcc
     4561 gatgtccttt ttccagctca ttccacattt gcaatgctgc aaaccaataa actgctgttt
     4621 cattctcctg ccgcataccg gacaccgctt attactgctt ccctttttct ttttcggcac
     4681 atcctccttt ttctccttcg gcggcttttc ttccggtatc ccattttccg caatacgaag
     4741 ctcatatcgt ggcaatttta aaagatatgc tcggtaaacc tcttcatacg gaacccagat
     4801 tgcaagagat ttcagttttt cataaatccg gtcaataatc tgctttgcta cctcctcatt
     4861 tggtatctct ccattttcct tatagaaaat gcaggtttcc cgaaacatcc agtctgctat
     4921 tttcatcttc tgcttctgtt ttaaatcctt ataggtctta ttcggctgtt tctgctgttt
     4981 atatgccagt gaatgatgat ttggtttcat acaatccctc tttcggacaa attctgttca
     5041 aagccattat aacaaaattt atgcctgtat caaatatttc cggtacattt atttcaacag
     5101 cataaccgta cacatctgtg catggaacag accattctcc accgtaacat tatcaagcag
     5161 caatgcctga aacaacacat tcctcacatt ttcaagactg acgattttct gttcattctc
     5221 cggcagatgt acaaactcca caccgtcctc cacagcagat tttcttgcgg cttcacaatg
     5281 agtaaaaagt cccagaatat acttatttga catggcaaaa gcataggttt tctgtccatc
     5341 atatacaatc tggtacttct ccgctgctcc ttccggcaga ctgaaaacgc actgcactat
     5401 ttctaaacaa accttacctg catagtccgg tttcagttta tttttatagc gttgtatttt
     5461 ccactggatt tcactacgct ccacagggga atgggtacgc ttaaactgaa ttttccggca
     5521 caacatatcc aaatccatat acacgttatt tcctacaaga tgctccgtat atcctctata
     5581 ttcccccaac cgcaacaaat acgaaagcac ctcattcagc ttttcctctg attctatgaa
     5641 ctgaaacggt ttttctccaa attccagata ttcaaactgt aatgggcagc tccctttctc
     5701 tatctcccgt tccataactc tttttacatc tcgtaatgat tccattttgc tcctttccgc
     5761 ttctggacat cgtgtccaaa agcaataaaa aaagggcagc tacaaatctt tcaattcata
     5821 actgccctga cgctgtttta tttcagttct atttctgcca tttttgcaaa tactgctttt
     5881 tgctctaaaa gctctttctg tttttcctcc ggcagacatt tgaagatttc ctcatatccc
     5941 atttccagca tcggcgttcc ctttgcgaag agatagagcc gggtatcaag ccattcctgc
     6001 cataagtcct caaacatact ctcactgtct gtataggtta taacatcatt aaatccatac
     6061 tccggtgtgt aatattgcag cttcacaaaa ccgtaccgtc ctacatccaa taccaaaatg
     6121 ctttccagct catacagttc tgcataggct gcggcaacct ttttgcaccg ttcccgttct
     6181 atgtctgtaa tgtaactttt ctccacagtt gcaaacctcc tgtttcgagg catcgtgctg
     6241 cctcttattc aatcttccgt gtttctattt caaaaacact tccgtccctt gtttttattg
     6301 taaaaatatt tgcggcatca gaggtatcta tatcctcaat ccctaaagcc acatcatttt
     6361 catctaatac ttcgtatagc tgatccttaa actcattatg ttccataact ttttactctc
     6421 cttgtatgtt ttatggtgcg tacacgcacc atattattat atgtttcatg cacttagaat
     6481 acaagtgtgg aaaggagctt tcagtatgat taaatacgat ccgctttggc gtacgctaaa
     6541 ggaaaaagga atcagccagt accagcttat caaagattat ggcattgata aagcacagct
     6601 tcagcggctt cggcagaacc ttgttgtaaa aacgcttatt ttaaacaggc tttgccagat
     6661 acttaactgc cggattgaag agattatgga atacgtcccg gatgataatt aaaggtgttg
     6721 ttctgattga tgaacaatgc ctttttcatt ttcgcttttc atctgcacct ttggacatcg
     6781 tgtccagaag tatcactgat tatctttatt atctccatca gcaccataac cattctcaga
     6841 attacaacca caccgccaat cagaccacct acaatacagt tcagtgccag tattcctata
     6901 cttcctgcaa ttccgaaatt ttttggtatg agaatcatac acatttttct gatcccataa
     6961 ggacagccga ccagtaccca atacaggaaa aaatcaaatc cttctgcttt tctgcaaatc
     7021 ggataacagg ttatcatcca aactgccagc aacgataccg gaagcacaat tttaaccaat
     7081 attcttttca ttccagccac ctccgtacat atcatgctgt acctcctgct gataataatg
     7141 attcatcgtt gagggtgcat tataaagagc tgttaccata tatgccttga tatttgcaat
     7201 tttcgtagtt gtttcctgca tacatcctat cacatattcc aaatgggagc tattcagttt
     7261 gaggaacttt gattttacaa gctcatatgg ataatcattt ccaccgattt taacagtctt
     7321 atgcttcaca catacaatct cgcagattac ctcatacaat tcctcataca gccctttgtc
     7381 ctgccatctg ccatatttca tgtgatgttc gtactcaata ttctctttga tgatttccat
     7441 gtaagcattg acatcttcca tcacatcaat ctccccttcc aactcactgc cggattgatt
     7501 gataggattg atataattcc tatcaatatt actctggtta gtatgattat agttagtata
     7561 attagggtct gctttttgaa cttccgcaag ttcagatatt gtacttcttg aagtaaaatt
     7621 tttagacttc tggaagtctg ctttttgaac ctcttgaagt tcagtttctg aacttctgga
     7681 agtataactt ttagaactct tgaggttcaa atcctgaact tctgtggaaa catcagcatt
     7741 tgcaggcgtt tcttcttctg cttctcctat tgtggcaaaa ttctttacat aaataatatc
     7801 cggttttcca agtcccctgc gtttcttttc gatcaatcct atcccattat gactgtccaa
     7861 ctctttcata atcttaacac aggttgcttt tgcacatccc aaatcctcca tgacattatc
     7921 aagagtataa atgatatagg ctctgttttc ttcatccaac cagccatttt tcatagacag
     7981 tgacatacgg tcaagcatca gaccgtacag gagcttggca tcgctgctaa gccccttgaa
     8041 acgctcgtct ttaatcagca accgaggaac acgataaaag gaaaactgtt ctgcttcaat
     8101 cccatagtaa taatcaaact gtatctgttt acccatttta ccgcaccccc tttactgttt
     8161 cttctgccat tcctccaata attgcaggat aatcccctca atctcttcgc tggagtattc
     8221 ctctgaaaaa aactggctga ttttttcaga cttgattgta actttcctct ctttcggctt
     8281 ttcctcggtc agtatcagac gcaccatcgg cagcgtcagt tcgccgcttt tcccatattc
     8341 cttcagcttg gcagactgta ctgtggaaat atttgctccg ctttcctcaa ggattaccag
     8401 cacccactgt tgtgcatctt ccgacagaaa ggaaatgtca acaccctgta ccagaccgat
     8461 tttctttctg tcaaccaact ctagcagctc atcggacagc cttgcaagcc agatataccg
     8521 ttgcaccgtc tttccgcttt ctccggcagc ttctcctacc tcgtcaagcg tatttccgtt
     8581 tccttttctg ccctgatgct tcatagcctc gtatttcatg gcataagctt tcgccttctc
     8641 acttggcaga atgtcctcac gctgaatgtt ggaatccacc atgataatcg tagcttcatc
     8701 gtctgtataa ttgcggacta tcacaggcat atcctctttt cctgcccgtt ctgaacctcg
     8761 ttttctgcgg tgtccggcta tgatttcata gccaccctct gccctcggtc ttgcaattcc
     8821 cggtacgagc accccatact tactgatgct ttccgttgtt tcttccattt tttcatcgtc
     8881 cagtacacgg aacggatgat ttctaaactc atgcaaatcc gtgagcttgg ctctgataat
     8941 ctgctcttcg ctccctgtct tttcttctgc atttccaaat aaatcatcat aactgcttaa
     9001 ttttaccttt gcggcacttc ctgctttact cattcccaag cacctcctcc gtcaaagact
     9061 gataagcagc ggctactttg cctttcggat catgttcgta gatactgaca ccctctgcgg
     9121 aaatctctgc cgcacggacg gaaatgggaa tactgttttc aaatatccgc accttgctac
     9181 cgtaattctc caccagtaat gcagtaatgt cctttgcata attggttcga gcatccacca
     9241 ttgtcagcag aatcccctca atcatcagct tcgggttaat ctgtctcctg acctttccta
     9301 tggtcttaat aagctgctgc aacccttttg ccggaagata tgccgcctgc actggaatca
     9361 gaatactgtc cgcacaggca aaagcgttta tagtaatcat gccgagagag ggcatacagt
     9421 caatcagaat ataatcataa ttatccttta ccagctctat gtaggaacgc atcacaagct
     9481 ctctgctcat cacatttaca agtgatactt ccagaccgga aagctcaatg tttcccggca
     9541 tcaggtcaat tccctctttg tgtttcagga taccataatc cgcttccata tcttcatcat
     9601 tgatgatatt gccaagtgct gttgcaagtg tttcctctag cttgtccggc tctgtgaagc
     9661 caagactgtc tgtcagactg ccctgtgcat cggcatcaat cagcaggact ttcttgccct
     9721 gttttgccaa tcctatgcct aaattactgg ttgtggtggt cttgcccaca ccaccttttt
     9781 ggtttgcgat tgcgattatt ttacacatga tagattctcc tttgttttct gctatttttc
     9841 tgctacgttt cttttcttta cttctacata cgccctgtaa aatttcttcg ttcccttatt
     9901 cggatacatc tcggaaaact ccgttacttc cactttcgga ttcctctgca aaatctttcc
     9961 gaaccactta atatcattct ttgttcccat caatctgact tttaacatta gtctgttcct
    10021 cccaataagg catacagctt atggttgtat gtggcatatt ccggataccg aatctgaatt
    10081 gcctgccaga aatatggtgc ataagcacag cagaatattt cctccaccgt gtactgcctg
    10141 cactcgtcca cctgttcttc tgcatcttca taatcggaaa cacttggtgt tccattgcaa
    10201 tacagattga atgccattct gatcactctc acacttccac tggtctgcca gccttcgtgt
    10261 aaacactctg ttattacgca tcctgttttg aaatcataaa ttctgtaaac atttcttctg
    10321 gtatcatcgc tgatcccaag acaatagcag agtgccttgt ggtaaacatc ctgataccgg
    10381 acttccttta acttctcgta gtagaatttc tcatgtgcat cgctgataaa aataatcttt
    10441 tcttcctttt tggtttctgc tcctaacgct gtacttgtca taatagaacc ctccttgtgt
    10501 tattttgatt ttgttttgac cataacaaca gaaaaaggac actctccgtt ttattggaaa
    10561 atgtcctttt aatagcattc aactattaag ttttgaaagg ttctgatttc taaaacatcc
    10621 cttttcagtc catattagtc caccgaatga cacaaatttg atgtcaattt gatgtcaaac
    10681 actcgataaa ctcataaaag tctatggatt ttctgattct tagtacaact tcgcacctgc
    10741 cggaatccgg tcattcacca tgagaagatt taatccctca tgtccatctt cctcgtgtac
    10801 tgctgaaatc agcataccct cggaatcaat gcccatcatc tttctcggag gcaaatttac
    10861 gattgcaatc gctgtcttgc caatcagatc ttctggctcg taatactcgt gaataccgct
    10921 taaaatcgtg cgtttccggt ctgttccgtc atccagagtg aactttaaaa gcttctttga
    10981 cttcggtact gcttcacatt ccagaatctt tactgcacgg aaatcagcct ttgcaaaggt
    11041 atcgaaatca atcatttctt caaacaatgg ttcgattttt acgttggaga aatcaatctt
    11101 ttctgcaacc ttttcctctg ccacagcagc attttctact gccttttcag ccttcccttc
    11161 gcccttctta gcgtctaagg acttcattgt tgggaacagc agcacatctc tgattgccgc
    11221 agagtctgtc agcagcatac acattctgtc gataccgaag ccgattcctc ctgtaggcgg
    11281 cataccgatt tcaagtgcat ttaagaaatc ctcgtctgta tggtttgcct cctcatcgcc
    11341 ggctgcaaac tgctcttcct gagccttgaa gcgctctctc tggtcgatcg ggtcattgag
    11401 ctcggagtag gcgtttgcca tctcccagcc attcataaag aactcgaaac gctcggtata
    11461 ttccggattc tccggctttt tcttggtcag cggagaaatc tctaccggat gatccataac
    11521 aaaggtaggc tgaatcagat gctcttctac aaaggcctca aagaacaggt ttaagatatc
    11581 gcccttctta tggcgctcct caaactctac atgcttttcc tttgcagcag ctcttgcttc
    11641 ttccaatgta tggatttcat taaagtccac accggagtat ttcttaaccg catctaacat
    11701 ggtaatgcgc tcaaatggct ttccaagatc catttccacg ccgttgtaga cgatctttgt
    11761 agtgccaaga acctcctgag ccacaaaacg gtacaggttc tcggtcagat ccatcatacc
    11821 gtggtaatcg gtatatgcct gatacagctc catcagggta aactcaggat tgtgtcttgt
    11881 atccaggccc tcgttgcgga atacacggcc aatctcataa actctctcaa gaccgcctac
    11941 gatcagacgc tttaagtaaa gctccaggga aatacgaagc ttcagatcct catccagtgc
    12001 attgaaatgg gtttcaaagg ggcgggcagc cgcaccgcct gcattggata ccagcatagg
    12061 agtttctacc tccataaagc cttctccacc cagatactta cggatagcag caaggatttt
    12121 agaacgctta ataaatgtat ccttaacatc ctcattcata atcagatcca catatctctg
    12181 gcggtagcgc agatctgtat ctgtaaggcc gtggaacttc tcaggaagaa cctgaaggct
    12241 cttggaaaga agggtaaact cacttgcgtg tacggaaatt tctcctgtct ttgtagtaaa
    12301 gactgttcct tttacgccta caatatcacc gatatccagc tttttaaatt ccttataggc
    12361 atcttccccc acactgtctc gggccacata tacctgaatg cgtccctgca gatcctgaag
    12421 gttgcagaaa gaagccttgc ccatgacgcg cttaaacatc atacgtcctg caatgcttac
    12481 ttccctctcc tcccactgat cataatgatc cttgatctcc atgctgtggg cagttacatc
    12541 atattttgtg atctgaaatg gatcctttcc tgctgcctga agctctgcca gcttatctct
    12601 gcgggccttc agtacatgat ttaaatcctg ctctgtctga tttgacttct gctctcccac
    12661 gtttcttctg ctcctttgtg aaatatttaa ctgtaaaatg tttcttatga tactctctgg
    12721 atctccagca ccttgtactg gatcacgcct gcctgtgtct ctacgtcaac cacatctcct
    12781 acggattttc ccatcagggc atggcctacg ggagcctcat tggagatctt gttctgaagg
    12841 ctgttggcct ctgtggagcc tacgatgaaa aattccatct cctcatcata ctctacatcc
    12901 agaagcttta ccttgcatcc gatattgatc ttatcaacat cgatctcgtc ctctactact
    12961 acctctgcgt tctttaacag cttttccagt tcttcaatcc gcaactcaat atctctctgc
    13021 tcatcctttg ctgcatcata ctctgcattc tcggataagt cgccctgctc tctggcctct
    13081 ttgatcttct gagctacttc ttttctcttt acaaccttca gattctgaag ctcatcctca
    13141 tactgtttta atccggcata ggtcaaaatg tgtttcttgt ctgccatgtc tcctgcttcc
    13201 ttcctttttc attctaaatt gccttaaaga cagcacattt ccggacagac ttaaagtcct
    13261 gcccggatat ttaaaaacgt ctcatctatg atgtattttt tgtttctgtc tgtataaaaa
    13321 agataccccg acattctgtg tattatataa gaaaaagcac ccattgtcaa gtatgggaag
    13381 ctatcccggc gcctgcccaa aaactaaacc atacgtccgt ccaaaagtgc tgccagccct
    13441 tcaagagtct ccacctggtt gatctcacac cgaagggagg ctgaatgctt catccctttg
    13501 gtgtaccagg ccatatggct gcgcatttcc cgcatagcca cattttctcc cttatattct
    13561 gaaagcatcc gtccatggcg caggatcatt tcccggattt cctccgtaga tggccttgga
    13621 ggaatctccc ctgtttccag atactgccgg atctcccgga agatccatgg atttcccttg
    13681 gcgcctcttg caaccataac gccgtcacag cctgtttcct gcatcatccg ggccgcatcc
    13741 tgagcatgaa agatatcgcc gtttccgatc accggtatct tcactgcctc ttttacctga
    13801 cggatcactt cccagtctgc cgtgccggaa taatactgct ccctggtcct tccatgaacg
    13861 gccacccagg ctgccccgct gctttctgcc attttcgcaa attcaactgc attgaggcac
    13921 tggtcgttaa agccctttct gaattttaca gtcacaggct tcttcactgc ctttaccata
    13981 gaagtcaata tcttttctgc cagctttata tccttcataa gtgcagagcc ttcaccgtta
    14041 ttcacaatct tcggcacagg acagcccata tttacatcaa tagccgcaaa aggaccgtcc
    14101 tcgatctgag ctgcgatctc agccatgatc tccggatcag agccaaaaag ctgcactgcc
    14161 acaggcccct ctgccgggtc caccgccatc agctcttttg tattcttatt tttatataaa
    14221 atggctttgg cgctgaccat ttccgtcacc gccattcccg ctccctgctc atggcacaaa
    14281 agcctgaaag gcagatcggt tactcccgcc atgggggcca gtatgatatt gctgttaagc
    14341 tcccgatctc ccagcctaag cttcatcctc atctctcccg ctctcttccc agtctacatc
    14401 ctcctctaaa aagaaggttc tgtagtataa ttccagcgcc tccagaagat cctttttctc
    14461 ttcctgatac tgtttgatct taaggacact tccgatttcg tcaaacaggt agtcgccttc
    14521 ctcagagact accttaaatt ccagacgtcc cctgtcgtca aattccagtg tcaggactcc
    14581 gcccacatcc tctgtaaaaa ggacgcacat aatatgcagc tcatcctgta ccgactgggg
    14641 aaggctctta aattcttcat taaaataata tttctgctca taggagctgg ctccgcagag
    14701 caccacttta tccatcatct tcttatctcc gcaaaggtcc gctgcccata cagacagcgg
    14761 acctcttttc tgcaattctt atcttaccag catctttgca atattaaact gctcttctga
    14821 gatatccttc ttcatattta atgcttcctg gatatcgaaa tccacaaatt cgccctttct
    14881 gtatgccacc agacggttgc tctttccttc taccaaaagc tctgccgctc tggctcccat
    14941 aatggacgca tacacacggt ccttgcaggt aggcgttccg ccgcgctgca tgtggcccag
    15001 aatggtagct cttgtctcaa tgcctgtggc agcctcaata cgctttgcca tagagctgga
    15061 atgaccgatt ccctctgcat tgatgataat gttgtgcttc tttcccctct tacggttctc
    15121 aatgatccgg ttgatcagtg cctgctcatc tccgtcgtaa cgctccggaa gcagaatatc
    15181 ctctgcaccg tttgcaatac cgcaccataa agcaatatat cccgcattgc gtcccattac
    15241 ctctacaatg ctgcagcgct catgggaagt ggatgtatcg cggatcttat caatggcctc
    15301 cattgccgta ttgactgccg tatcaaagcc gatggtgtaa tcggtacatg caatatccag
    15361 gtcaatggtt cccggaaggc caatggtatt gatccccagg gcggacagct ttcctgctcc
    15421 cttaaaggaa ccgtcgccgc cgattaccac catgccgtca attccgtgct tgcggcagat
    15481 ctcagcacct ctctgctggc cctcaggagt ggtaaattcc ttgcatctcg ccgtataaag
    15541 aatggttcct ccgcggttaa tggtgtctga aacactggtg gactccatgt ccacgatctc
    15601 ctcttccaga agaccggcat aacctctcat aatgcccttt accttcagtc ctttattgat
    15661 cgcaacgcgc acaactgcgc ggattgctgc gttcatgcct ggtgcgtcgc cgccgctggt
    15721 taatactcca atggtcttca ctgcctttgc catagcaatt tacctcctta tattaagcat
    15781 atccatctat gcctgttgtt ttcctctttc tattttacac acaacgctat tctaattcat
    15841 tttccatgat ttatcaagag ccatttctac gactttgaca tttttttcac caactttaca
    15901 atacaggcga tttaaaagtc ccgcatccgc cattacgttc cagttgggag ggagcttttt
    15961 tttcgcccgt tcctttttta aatagatgac cacctgatca tttccctcgc tggattttag
    16021 aagctccata agctctgcct ccgctgcctg atacccggcc atatcttcaa actgcagcca
    16081 cagctgacgg ggcacagcat caaaggggat cacctgctcg cagaccagtt tgcccaccgg
    16141 ttcatctccc agtgaaacac gtccccggat aaagagcttt tcatcctctg tcagaagatc
    16201 cctgttattt tcatagtctc ttggaaatac gatcacttcc actgatccca tcagatcctc
    16261 cagcgtgata aaggccatca gctggcctgt gcgggtagtc ttgaccgttt ttcccgttac
    16321 caatccgcct atgacagcct ttatgccgtc ctttaccacg gcctcctccg tctcctcatc
    16381 cacgataaaa tcagctgctg tagcagtgat atttttccgc cacagcgtct cataatcatc
    16441 cagcggatgg ccgctgatgt atacgcctaa aatatctttt tcaaaggcaa gcagctcttc
    16501 ttttgcatat tcccctacat caggaaatac aacctcaaac tccttttttt cctcttctcc
    16561 ggctatatca aaaaggctca gctgtccctc aaaggcattc ttccgctctc tggccttatt
    16621 ctcaagaagc gcaggcccca cagccatctt ctgccgtctt gtgccgggaa gagaatccag
    16681 tgcgcctgat ttgataaaat tttccagcgt acggcggttt acctctctct gggtcatacg
    16741 gttgataaaa tcctccatgg tacggaattt tccattcttc gcccgttcct ccagaatggc
    16801 gtctaccacc ggctttccca cgcttttaat cgcagaaagg ccatagcgga ttcctccgcc
    16861 ggacacggaa aatccgctct ctccctcatt gatatcagga ggaagaactg cgatattcat
    16921 catgcggcgg cagtttaaaa tatattcgga aaacttgctc acattttcca tcacagaaga
    16981 cataagggcc gccataaact cctttggata gtagtatttc agataagcgg tctggtagga
    17041 aactacagca taggctgccg cgtgggactt attaaaggca tactttgcaa agtcgatcat
    17101 ttcatcataa atatgattgg ccgtcttttc atcaatacca tttgcaatac agccctttac
    17161 tccctcggct tcatttccgt aaacgaaatt ctgacgctct ttttccatca cagcagtctt
    17221 tttcttactc atggcacggc gtaccaggtc gcttcgtccc atggtatatc cggccaggtc
    17281 gcggacgatc tgcattacct gctcctggta tacaatacac ccgtaagtcg ggctcagaat
    17341 cggctccagc tgagggcatt cataggtaat ggagctttta tcattctttc cttttaaata
    17401 cctgggaata aagtccatag ggccgggacg gtaaagggaa attcccgcaa taatatcctc
    17461 cagattctcc ggcttaagct ccttcataaa gctcttcatc ccgccgcttt ccagctgaaa
    17521 cacgccctcg gtccttccgg ttccgatgga ttcaagtacc cttttatcat caaaatcaat
    17581 atggtcaata tcaatggaaa tgccgtaatc cttctcagcc agatgcacgg cattctggat
    17641 cacagtcagt gtacgcaggc ccagaaagtc cattttaaga agccccagct cctcaatggt
    17701 cgtcatggta aactgggtgg taatggtgcc gtctgccgcc ttggaaagcg gtacaaattc
    17761 gtcaatggag cggctgccga tcaccactcc cgccgcgtgc atggaggtat gtcttggaag
    17821 gccctccaga cgctttgaca tatcgatcag gtattttacc tgtgcgtctt cattataaag
    17881 ctttcttaaa tccggatttg ccgctatggc cttgtcaagt gtcattccaa gatcatttgg
    17941 gatcatcttt gcaatggaat cacacatggc gtagggcaga tccagcacac ggcctacatc
    18001 acggaccaca cccttggctg ccagagtacc gaaagtaacg atctgaacca cctggtcttt
    18061 gccgtatttc tccaccacat aatcaatgac ctcctggcgg cgctcgtagc agaaatccac
    18121 gtcaatatca ggcatggaaa ctcgctccgg gtttaaaaag cgctcaaaaa gcaggttata
    18181 gcgcaccggg tcaatattgg taatgcccag agtataggaa acaatgcttc ccgcggcaga
    18241 accacgtccc ggtcccacca taatatcctt tgagcgggca tagttgataa aatcccatac
    18301 gatcaggaaa taatccacat accccatggt acggattaca tccagctcat agtccaggcg
    18361 tcggcttaac gtgccgtcat cctttggata tcgggtctta aagccctccc ggcagagatg
    18421 attcagatac gtccaggagt caaagccctc cggcacctca tactttggca gcttggtgac
    18481 gccgaattca atctccacat tgcagcgctc tgcaacttta tgtgtatttt caagggcttc
    18541 tctggcgtat gggaaaaggg cctgcatctc ctcctcggat ttacagtaat actggccgcc
    18601 cgcatagcgc atccggtctt catccgctac ttttttgccg gtctggatgc acagcaggat
    18661 atcgtgagcc ttttcatcct ctgcaaaggt ataatggata tcattggtgg cgaccagttc
    18721 aatccccgtg tccctgctca gcctgagaag tccctgattt actatcttct gttctggaag
    18781 cccatgatcc tgaagctcca gaaagaaatt atttttccca aatattccct cataccgctg
    18841 tgcagaacgc atggcctcct catacattcc tctggccaga tatttctgca cttctccggc
    18901 cagacaggcg ctgagggcaa tgactccttc gtgatacgtt tccagaacct catagtctac
    18961 acggggctta tagtaaaagc cttccacaaa gcccttggat acgatcttca tcaggttttt
    19021 ataaccctga tcattttctg ccagaagaac cagatggtaa taccggtcct ctcctcccgt
    19081 attttcccgg tcaaacctgg atccaggagc cacatatacc tcgcagccca ggatcggttt
    19141 aattcccact tcctttgccg cccggtaaaa atcaatcact ccatacatta cgccatgatc
    19201 cgtgatcgcc aggctgtcca ttcccagctc ctttgccctg gcggtcagct cttttatctt
    19261 ggatgagccg tctaaaagac tgtattctgt atggacgtgc aaatgtgtaa acgccatata
    19321 tactgattct cctttcccgg aacaacgtcc tgttcctgtc tgttatgaaa aaaaccaggc
    19381 agccttctgc actgcctggt ttccaactat ccttttaatc tctctgaaac acttatgcat
    19441 tgatgagata cttctcaatc tcagacactg cctgatcctc atccgcaccg tcagcctcta
    19501 ctgtgatgga ctggccggca atcagcccaa gggtcatcat tcccataata ctctttgcat
    19561 ttacacgctt ggaaccggac tccagataga ttctgctgtc gtactggctg gcaacctgca
    19621 ccagcatagc ggccggtctt gcttctaatc catcgggcag ttcaacggta attatttttt
    19681 tcttcatgat tatcctcctc atatctgcgg atggagcgtt tccttactgc tccgggaact
    19741 tttcccttaa atcctccgcc ataaggctga gttttctcag ccggtgattg actcctgact
    19801 ttcccaccgg cggctccaga gcttccccca gctccttaag tgtcgcctcc ggccgttcca
    19861 gcctcactct tgccatttcc tgaaggttct ccggaaggga ctcaagtcct atggtatcct
    19921 gaatataccg gatatcctcc atttgcttta cggctgccga aaccgtttta ttgatattgg
    19981 cagtctcgca gtttacctga cggtttacgc tgtttcgcat ctcctttacg atccggatat
    20041 tttccagctc cataagcgcc accggcgcct ccatgatctg aagcagctct gcgatctggc
    20101 ttccctcctt tacatacact acaaaatagc gtttgcgcag aacgatcctg gcctcaatat
    20161 caaatgaagc catcagcccc tgaagctgct ttgccttccg catggatgca caggtgatct
    20221 caaaatggta gaacttttcc ggatcactga tggaacccgc cgaaaggaag gccccgcgaa
    20281 taaacgcccg cttacagcag acattctgca ccacaacgtt atgtatcagc gacagatcct
    20341 ccgcaatttc tccataagaa tccaaaagtt tggacgcctg aagaacccgg agcgcatcct
    20401 catgctctct cactgctacc atatatgttc gactccgccc cggcatctgg ccccggcgga
    20461 ttaaaacatc cgtatttata ttaaatgttt ttttcaataa tgtaaagtat tttcttgcaa
    20521 ctgccagatt ttctgtatgg atctcaatgg catagtgatc ggaggccgat atctttaccc
    20581 ggccgcagag gcttaaaaca gccgctgttt ctgcgatctg gcagtgcctt gccggagacc
    20641 actgtctgga cagttcctcc tttactttgg aagaaaatga cattgctccc tcttaacttt
    20701 ccctgttatc gtttactatc cttatcaata tctctgtgat ccatgcggat cccatattct
    20761 ccgtggctct tcaaatggtc atacagagcc tttgcaatgg tgacagaccg gtgctttccc
    20821 ccggtgcatc cgacagcgat aaccagctga tttttccctt cattgatata attgggaaga
    20881 aggaaatcca tcatgtcatt cagcttctct ataaacgtct ctgctgtatt cccctgcatc
    20941 acataatctc tcacagactg gttttctccg gtcaggggac gcagctcctc cacatagtaa
    21001 gggttcggga ggaaacgcac atcaaagacc agatctgcat cctcaggaat accatactta
    21061 aagccaaagg acataacggt aacaaacata tttttaaagc tttcttcctt taaaaatatc
    21121 ttttccagct cctgacgcag ttcctttgtc aaaagcatgc tggtatcaat aataaaatcc
    21181 gattccttct ttaaaaacgc cactctggcc cgttcctttt caatgcctgt ttccagccgt
    21241 ccctttccgg ccagaggatg ggaacgtctt gtttccttat agcgtttgat caaagcggta
    21301 ttgctcgcat ccagaaaaat gatctgtaca tctatctgtt ctctgcgcca cttttcgata
    21361 acgccttcca gatcttttag accctccccg ctgcggatat cgatccccag agccgccttc
    21421 tgcacagttc ctccgtccga taccagctgc acgaatttat ctgccaggga gataggcaga
    21481 ttgtccacac agtaaaaccc cagatcctcc aacattttta aagcaatggt ttttccggcc
    21541 ccagacattc cggtgacaat tacaagcttc atctgtcagt tcacctcgta ctctttatcg
    21601 taatccacct taaaatttcc ccagtgtctt tacttccatt tccagctcca catcaaactg
    21661 tgtctttacc cgctcccgaa tctgacggca gagttccatc acatctgccg ctgtggcatc
    21721 gcccctgtta atcacaaagc cgctgtgctt ttgggacacc tgcgcgccgc cgacggtaaa
    21781 gccttttaat ccggcatcct caatgagtct ggccgcaaaa tgcccttttg gccgtttaaa
    21841 ggtactgccc gcactcggat actgaagagg ctgcttttcc ctgcgtttta aagacagttc
    21901 attcatatac gcctcaatct catcccgatt tccttttttc aggcgaaagc aggcgtccag
    21961 cacggtataa cccatagcag ggatacagct ggtgcggtaa tccatcttaa gctcctttgg
    22021 cccaagagta aggatttctc cctccggcgt cataactctg gcatactcca gtacatcctt
    22081 tatctctgac ccataggccc cggcattcat caccagagcg cctcctatgg ttcccggaat
    22141 cccggccgca aattcaagac ctgtaagccc ctcctttaaa gctcttttcg ccgttcggct
    22201 taaaagctgt ccggatgatg caaaaatcct ctctccttct gcctcaagac gcgtcagccc
    22261 ctctgtcgtg gaaataacca ctccctcata tcctgcatca ctgaccagca ggttgcttcc
    22321 atttcccaga atgaaaaacg gaagcttctc ccttctgcag aaagagagga ccgttttaag
    22381 ctctgtctct ccggacggac atacataagc cgctgcggga cctccgatcc gaaaggtggt
    22441 gtggctgctc ataggctcca taaaacggca cggaaagccc tccagctcca aaagagcctg
    22501 cagtttttct ttaagctcca tctgtttcct ttctccggtc tgaatatcag tcattcatcc
    22561 cgttttttaa atttgcctgt acacggttat acagctcctg agccgcattg tagcccatct
    22621 tcttctggcg gtggtttaca gccgccgact ctacgattat ggcaagattt cgtccgggac
    22681 ggatgggaat ggtgtggcac acaacctgat tgcccagaaa ttccgtatac tgatcttcca
    22741 ggcccaggcg gtcatactcc ttatcccggt tccagtcctc cagcttgatc accatatcga
    22801 tcgactgtgt atcctttaca ctctcaacac cgtacagagt ctttacatca atgatcccga
    22861 taccacgaag ctcaataaaa tgccgggtaa tatccggcgc acttccgatc agggtatcgt
    22921 cgctgacacg gcggatctcc accacatcat ccgtcacaag gcggtgtccc ctctttaaca
    22981 gctccagcgc cgcctcactc tttccgatcc cgctctctcc catgatcagc acaccttcac
    23041 caaatacgtc aagaagcact ccgtggatgc tgatgcaggg agccagcttc acattgagcc
    23101 agcggatgac ctctgccatc agggatgagg ttgtcttatc tgatacaaga cagggaacac
    23161 cgtaataatt gcagtattcc agcagatcat catcgggatt ctgtccccgg ctgaatacaa
    23221 ggcagggaat ctggctggag gtcagtttat catacatctg acgcttcact tctctgtcca
    23281 tggtattgat atatgcctgc tccacgtatc ctatgatctg tacgcgctct ctgtcaaaat
    23341 gatcgaaaaa tccggtaagc tgcagcgcag gacggtttac atctggatgg gtcagtacga
    23401 tcttatccgt atcaatctcc ggtgtggtgt tgcgcagttt cattttctca ataagctcac
    23461 tgatggttac tccatgcatg gtataattac ccccttgtgt ctgctctccg cacatggacg
    23521 cggaaaggct atctggttaa catatatact atcacaagtt tcactgattg tcatgtgttt
    23581 ttacatccgc gcacattccg ccgcagaacg aatgcccccc tgttttacga aaaaaatggt
    23641 aaacggcctc tgcggccttc tgattcatct gaggcgtctg ggccagctcc tggatgtctg
    23701 catttcttat ggcctcgata tccttaaact ggcgcatcag cgccttccgt ctggcagggc
    23761 cgatgcccgg gatttcatcc agtaccgatc ttacctgccc ttttccccga aggcttcggt
    23821 gatattcaat ggcaaaccgg tgggcttcat cctgaatccg cgtaataagc cggaagccct
    23881 cgctgtgccg gtcaataggc acctctttat tgttgaaata caggcctctg gtgcggtgaa
    23941 aatcatcctt taccatgccg cataccggaa tggaaaggcc catctgttca agaactccaa
    24001 gggcaatatt gacctgtcct cttccgccgt ccatcatgat cagatccgga aaacgggtaa
    24061 agcttccaaa agaaagttcc tttccctccg ctctgaggtt ctttgcctcc tccattccat
    24121 gagaaaaccg tctggttaaa acctcttcca tggaggcata gtcatttggc ccctggacgg
    24181 tgcggatctt aaatttcctg tagtcactgc gctttggctt tccgtcctca taaactacca
    24241 tagaacctac cgactccacg ccgcttgtat tggaaatgtc aaaggcctcc acccggcgca
    24301 ctgcaccgat cccaatgagc gctccaatct ggttcatggc tcctatggta cgcagttctt
    24361 cctttttgat ccgttcctta tcctgagaca gaaccagagc cgcatttctt gccgccagct
    24421 ccaccagcct ttccttctcc cccttctggg gcaccaccag ccttaccttc tgtccccttt
    24481 tcgcggtcag ccactggctg ataacctcgc tgtctgtaat gggatactga agccaaagct
    24541 cccttgggat aaacggcgtt cctgcataaa actgctttac aaagccgtcc aaaatctcct
    24601 cctcgctctc tgccgctcct gctgacaaat gaaaatgatc ccttccgatc agctttcctt
    24661 ccctgataaa aaatacctgt accacagcgt ccctttcgtc tcttgccaga gccacaatat
    24721 cacggtcctc catgctgctg ctggtaattt tctgcttctg tgccacctgc tttacactgt
    24781 ttaacagctc ccggtattca atggcctttt caaactccat ttgctccgac gcctgctcca
    24841 ttttctcctt cagggaacgc agaagaggct cgtaatggcc tcctaaaaaa tcaagcgcct
    24901 gtccaaagga cttctgataa tcctcctttg aaatataccc ctgacaggga gcatcacact
    24961 gcttgatgtg gtaatttaag cagggcctct cctttcccgt atccttcggc agattccggc
    25021 tgcaggtgcg gatccggtac agcttatgga tcaaatccag cgtatccttg actgccccgg
    25081 cgctggtata tgggccaaaa taccggctgc gcccatcctt tttcatatcc cttgaaaata
    25141 aaagtcttgg gaaatcctca cccaccgtag ccttgatgta aggatatgtc ttatcatcct
    25201 ttaacatggt attgtatctg gggcgatgct ccttgatcag attgcattcc aataccagcg
    25261 cttccagctc tgaatcggtt agaatgtact caaaccaggc aatatgggaa accatctgtt
    25321 cgatcttggc agtcttattc cggctgctct gaaaatactg gcgcacccgg ttttttaagc
    25381 tgactgcctt tcccacatag atgatctcat ccctgtcatt gtgcatcaga taaacgcccg
    25441 gactggcagg gagttttttt aattcctctt ccagatcaaa catcaaaacc ctcctgttca
    25501 gcgcagccgt gtgccgtcat agcggcgcag aagctgtaca tagcggcgga gcgcctgaat
    25561 ctgctcctcc acaggcattt ccggctccgg ttttaaatcc atagccataa acatacaatc
    25621 cagctcccgt ttggttgcct ttccttccat ctgtttcaaa acagctaact tatcctcagg
    25681 gcgcagcgca tccaaaaaac gctccagcca ggggcctgga agttcctctt ccttatccgt
    25741 ctccccctgt tccctttcct gccaggcagt tcttacagga tcattctccg gccatacacg
    25801 ctcaaaacgg tattcctgat ccgcatccgg atatttttcc cggtctaccg gactcataaa
    25861 catctccaga gggcggacat acataccaaa cgttccatac agtgcctgat acaccaccat
    25921 aggctctcct gtttctgaat gaacagccac tgctatgacc tgatacagtt tatttttaaa
    25981 atgcctgtaa aatgctcccg gcacaggcat acactctctc tgctgcaaaa tgcatctacc
    26041 tcatttccgc ccggcctatg ccgaactctg tgtttatctt tttagtatac catatctcat
    26101 tttagaatca acggaatttt gtccggacag ataaaaacgc cccgctgcta tgctgtctgc
    26161 aaagctgcgg ggcgtacacc tgggtctgaa aaggtatgct tctgcattcc ttactgtaaa
    26221 gaagctgccg cgtcttttcc tgcagctaaa tattcctgac cggaaaattc atctgcattc
    26281 gccgctgccg catttacaag aatcagcact gctgccagaa tcacccaggc aaagcattcc
    26341 aatccttttt tcagtttcat aatcctcttt tcctcctaag gaccacatct ggatccgact
    26401 cacattatac cgtttttccg caaaaatgca agttttaaat ttttttctga cagacgggga
    26461 atttctctcc ttcatccgtt ttatatagta gaggctgtta attttatact tctgataagg
    26521 aggaatattt atgattactt ataagccatt ctgggaaacc ctgaaaaagt cacccgaaac
    26581 cacctatacc ctaatccata aacatcaaat ttccagtgct accattgacc gtctgcgaaa
    26641 ggataaaccc ttaagcacaa tcaccattga cgatctgtgc catattctga actgccaggt
    26701 gggtgaaatt gccgcgtaca tacctcctga agataaacca cattaaacgg ttaaactctg
    26761 aacattttac aatagccttc tgttttttcc tgtgctatac tttaagaagc ttaacgtata
    26821 aggaggtttc tttgtaatgc agaagattgg aagaaatgat ccctgctggt gcggcagcgg
    26881 taaaaaatac aaagactgtc acatggagct ggaagaaaag ctgacccagt tccgtcgtca
    26941 gggcgccatc gtccccacca gagccttatt aaaaacaccc gaacagatcc gtggcataaa
    27001 agagagtgcc cggatcaatg tagccgttct tgattatgta gctgcacata tcggccccgg
    27061 catcactact gcccagatcg atctgtgggt ctatgaacag accacccgtt taggaggcat
    27121 tcccgcgcct ctgggctata acggctttcc cagaagcgtc tgcacctctg tcaatgacca
    27181 ggtatgccac ggcattccat ccgaaaatga cattctgaaa gaaggcgata ttataaacat
    27241 agacgtctct actatctata atggttattt ttctgattcc tcccgtatgt tctgcatcgg
    27301 agcggtttct ccggagaaaa aacgattggt agatacagca aaacaatgtc tggagattgg
    27361 tttgacagaa gtacgtccct ggggattttt aggtgatatg gggcaggcaa tccataattt
    27421 tgccaaagaa aacggctgca gcattgtccg tgaaatcggc ggtcacggcg tgggactgga
    27481 attccatgaa gaaccctggg taagctatgt gtccaaaaaa ggtacagaaa tgctcatggt
    27541 tcccggaatg tgctttacca ttgaacccat ggtaaatatg ggacggccgg atgtatttgt
    27601 ggatgcgaac aacgactgga cggtatatac ggatgacgga ctgccctccg cccaatggga
    27661 aatccaggtg ctgatcacag aggatggata tgaaatactg gcgtggtaga cgattttccg
    27721 aatagagtat gcaacacgaa atgcagcacg cattaaaaaa agtcctgtaa tacaggactt
    27781 ttttaatgga gatagtggga ttcgaaccca tgacctcttg catgccatgc aagcgctctc
    27841 ccaactgagc tataccccca ttggaacata tttatatcaa ctggcgtcga ctcgttgata
    27901 ataccatatc cggcagaata aatcaagtat tttccaaact tttctcaaaa ggtttccaag
    27961 acacaagctc cccgaaaggt tttcgccttc cggggagctt ctatcattta atatacattt
    28021 tcactgtaga aattattcat ttccatacag agcaaccagc ttggataagt gctcaaagcg
    28081 ctccttagcc tcctgctcgt tgcgtgcgaa cagctctgct gctctctcag gattcttcat
    28141 ggtcagagac aggtaacgaa cctcgccctt taagaagtcc tgatagcctt ccatcttagg
    28201 agccttggaa tccagagtga acttcttctc tgctgcagga ttgtaacgga agttattcca
    28261 gtagccacac tctaatgcca gcttctcctc agtctgagcc ttgttcatgc ccttcttgat
    28321 accatggttg atacatggag catatgcaat gatcagagat ggtcccggat atgcctcagc
    28381 ctcagccagt gccttaacgg tctgctgcat gtcgccgccc atacagatct gtgcaacgta
    28441 tacatagccg tagctcatag cgatgccagc cagatccttc ttcttggtct ccttaccgcc
    28501 agctgcgaac tgagcaactg caccggtctt ggtagccttg gaagcctgtc cgcctgtatt
    28561 ggagtaaacc tcggtatcgt ataccatgat gttgatatcc ttaccggaag ccagaacgtg
    28621 gtctacgccg ccgaagccga tatcgtaagc ccatccgtca ccgccgaaga tccactgaga
    28681 cttcttagcc aggaaatcct tgttagctac gatgtccttg caaaccgggc agtcaacgcc
    28741 ggataatgca gcaaccagac gatcggttgc agaaccgttc agagcgccga tgccataggt
    28801 atctaaccac tccttgcagg ctgccttgat atcgtcagtt gcgttctcat ttgccataac
    28861 gctctcaacc ttagccttta agccgtctct gattgcgttc tgagccagta acataccata
    28921 accgaactca gcattgtcct caaacaggga gttatcccat gcaggaccct gtcccttagc
    28981 gttcatggtg tatggtgtag aaggtgagga gttgccccag atagaggaac atcctgttgc
    29041 gtttgcaatg tacattctat caccaaacag ctgggtgatc agcttagcgt aaggagtctc
    29101 gccacagcct gcacatgcgc cggagaactc aagcagaggc tgcttgaact ggcttccctt
    29161 aacagtgttc tctttgaact ttgcaacaac ctcatccttg atggatacag tctggccaaa
    29221 gtcaaagata gcctgctcgc tgaggctctc ttccagaggc ttcatctcca gagccttgtt
    29281 gcccttcatg cctgggcaga catttgcaca ggagccacat cctgtacagt caagtgcaga
    29341 gatggtcatg gcaaacttgt agcctggcat accggtcata tccttcatct tcatatctgc
    29401 aggagcgtta gcagcttcat cagctgtcat agctaccgga cggataacag cgtgaggaca
    29461 tacataggaa cagaagttac actggataca gttgtcaaca ttccatacag gaacatttac
    29521 tgcaatacca cgcttctcgt atgcggaaga accagatggt gtagtaccgt ctacataatc
    29581 cttgaatgcg gatacaggca gggagtttcc ttcctgagcg cttaccttgg tctgaatgtt
    29641 gtttacgaag tcaacaactt ccttacgtcc ctcggtaact accttataat caaggccttc
    29701 gtcctcgcac tgagaccagc ttgcaggaac ctctaccttg tgaacgccct tagcgccggc
    29761 atcgattgct gcccagttct tcataacaac gtcctcgccc ttgcggccgt aggttgcctt
    29821 tgcagctgcc ttcatcagtt catttgcctt ctcagcagga atgatgcctg tcagctcgaa
    29881 gaatgcggac tgaaggatag tattgatacg ggtcgggccc atgccggtct cgataccgat
    29941 cttaacaccg tcgatggtgt agaagttgat cttatgatca gcgatgaact tcttaacctg
    30001 tcctggcaga tgcttctcaa gaccttccat atcccatggg cagtttaaca ggaaggtacc
    30061 gccgtcaacc agctcctgaa ccatgttgta cttgcgtaca taagaaggat tgtggcaggc
    30121 aacgaagtta gcctgacgga tcaggtaggt agacttgatg cggctgtgtc cgaaacgcag
    30181 atgagacatg gtaacaccgc cggacttctt ggagtcgtaa tcaaagtatg cctgagcgta
    30241 catatcagtg ttatcaccga tgatcttgat ggagttcttg ttagcaccta ccgtaccgtc
    30301 agcgccaaga ccccagaact tacagttggt tgttccctct ggagtagtaa ctaaaggagc
    30361 gccaatctcc agagacagat ttgtaacgtc atccacgata ccgatcgtga atgggctctt
    30421 tgtctcattc tcaaatactg ctacgatctg agcaggtgtg gtatccttgg aacctaagcc
    30481 gtaacgtccg gtctggatct tgatctggtc aaacttgctg cccttaagag ctgcaacaac
    30541 atccagatac agaggctcgc ccatagcgcc tggctctttt gttctgtcta atacagtgat
    30601 ggtctttaca gactcaggaa tggtatcgat cagtgcctgt gcgcagaatg gtctgtacag
    30661 acgaacttta acaacaccaa ccttcttgcc ctgtgcaacc aggtaatcaa tggtctcctc
    30721 gatggtatca tttacagaac ccatagcaac aatcacatgc tctgcatcag cagcgccgta
    30781 gtaattgaac agcttgtaat ctgtaccgat cttagcgtta accttgtcca tgtactcctg
    30841 aacaattgca ggaagtgcat cgtagtaggg gttacatgcc tctcttgcct ggaagaaaat
    30901 atcagggttc tgagcagaac ctctctggca tggatgattt ggattcagag cgttcttacg
    30961 gaatgctgca atcgcatcca tatcagccat atccttcagg tcctcgtaat cccaggtctc
    31021 gatcttctgg atctcgtgag atgtacggaa accgtcaaag aagttgatga aaggaacttt
    31081 gcccttgatt gctgccagat gagcaactgg agttaagtcc ataacttcct gtacgctgga
    31141 ctcacacagc atagcgcatc ctgtctgacg acaggcataa acgtcggaat ggtcaccgaa
    31201 aatagataat gcatggcttg ccagtgcacg ggcagatacg ttaaatacgc ctggcagctg
    31261 ctcgcctgcg atcttgtaaa ggttagggat catcagtaac agaccctggg aagcagtata
    31321 ggtggtggtc agcgcacctg ctgccagaga accgtgtaca gcacctgctg caccagcctc
    31381 agactgcatc tctgtaatct gaacttcatg accgaagatg ttggttcttc cgtctgttgc
    31441 ccattcatct gtagcttctg ccataggtga ggatggggta atcggataaa tagccgctac
    31501 atcagtaaat gcataggaag catgagctgc tgcatggtta ccatccatgg ttttcatttt
    31561 tcttgccatt ttgatttcct cctgcaatta gtgaaatatg tatatgatag tatacatgta
    31621 agtccgcccg acggcacata gcctttagcg gactgacctg tatattatta taaacaattc
    31681 tttaacaaat tgcaaatgct taattgctat tactgcaaaa aatacaatgt atacagtata
    31741 atttatgcat aaatcctctg acagccagaa atagcgaaaa agaagcagcc gtaaaatttc
    31801 catttgggga ttttacggac tgcttctttc ttaaaatttt taaaaattca aaccgctatt
    31861 ccggctttac gggaacaacc acggctccgg ctcccggccc gccctgtgaa ccggaaggcg
    31921 cgtcctctga cggagcaggg gccacaggta cggaagacgg ctctgtagaa gctgatcccg
    31981 gcccttctgc cggagcagag gaagccgtac ctgacccagg ctgggactgg gtcgtctcag
    32041 cagggccggt atatccctcc ggcattccgt ccactgtcgg cgtaaccgtt gttgcctgcg
    32101 tggtggtttc ctccggattt aataccacac cgctggtatt gcggtggact accggtttgt
    32161 ggcccttata cgttgtttta tgatccagtt ctctggaaat ctccttgccg tccttataga
    32221 caaccttata ggtttcccag cggcttccgg agcttccctt gcttgtggtc ttttccactc
    32281 ccggatccag agtctgatct tctacatatt ccggttccgg cacaccaagg tcttctacct
    32341 ttttagactc cagatcccag gtgatgccgt cctccagaat cgggatacca tatacagata
    32401 ccgtcatctt ctgatccgca tagcttgccc gcaggccgat cgctgtgctt gaggtattga
    32461 tgaatttaaa atcaggctgc tcataactta cagtagcatc tcgtcccgga gttacataat
    32521 ttggctcaaa ggtatgggaa cggcgatagc tgatctttaa gcccgcttta aataccgcat
    32581 tgtacagagt agttgaaacc tggcatacgc ctccgccgat ttcctgaaca acctcaccgt
    32641 tattataggc agccgcaccc ttataaccct tagcctctgt acgctggcct acagtttcgt
    32701 taaaggagaa ctcatggccc ggcttgataa cggtgccgtt aatagcttct gccgccagac
    32761 ggacattcgt atttctgttt ttattggatg tggtatttgt agtaaaggaa ccaatggtct
    32821 tatactgctc ctttgcgctg gccgccgtaa tctcaggtgc caccgtgctg ccggatgcct
    32881 caattttggc atcaaagtcc tttgcagcca gagcctgtgt gacatcatcg atcagctttt
    32941 cctgatcaat ggcaaagcca ttctcctctc ctgcgaaaac gaacttccct gtttctgcat
    33001 taaagctgtc gatggaacca ttttttgcct tcttatccca cttcgccgcg caggctgctg
    33061 cctccgcttt gatctgctct tctaatccgg ccgtatccag cgtgtagctt tcctgaggct
    33121 ctccgctgta gatctcattt aaaagagaat cgatttttcc ggccatcagg tcatttacct
    33181 cataaacttc agaatcataa gaaacggtca tgctccatgg aaattctttt aacagcacct
    33241 ctctggcctg agatttggaa aggccggtga tggaaatgcc gtctacggac acctcttttt
    33301 tcatctcggt ttctgtcact gcctctgttg ttgcctgcac tcccgtctca tcagagttct
    33361 ttccatttaa cgccatagcg atgcaggtaa tcactacgat caggatcacg cctccaatgg
    33421 caatccattt ataatccggc ccctttcttc tgtggcggcg gctgttccgc cttacattct
    33481 gcatggcgtt catccttcct gcctggccgg agcggtaaga ggaactgctg cgtgagccgc
    33541 cgtgtcctgt atttctggaa gaggtacctc tggatgatgc tctcccggaa gcactacgtc
    33601 ctctgtctgc gccctttgcc cggcttctgc ccggcgaagc gctgcggttc ctgctgttgt
    33661 tttctaactg attcattttt ctcacacctt gttcaaaata cttttgcatt aattacagct
    33721 tgtagtatag catactttat ttaaaatgga accgcttttt atattttttt cctttgattt
    33781 tttctatttg tgaatttact gttacttatt ataaatgaaa tattatggaa gtattaccaa
    33841 agtatttcac tgctcttttt tagaggctgt gacctttgaa agctgcaaaa tgatctcgct
    33901 gaaatatctg ggcggaacct gctccagaag aagcgccttt ttatcatctg cctcatcata
    33961 gacatagctt cccctctcta tggttccggc cagatagaaa cggacgtctt ctattgttac
    34021 ctcctcgttc tgtcctccga tataggttcc tgaaaaatga agctccgctc ccagcttccc
    34081 gtcttcccgg taaaaggtat aaaaacagcc cgcatcctga acagaacccg tataccagcc
    34141 aagaggctgc atttttccga tgagagacag atcgttaagg attactcctt caaagcgctc
    34201 cactgccttc tgccccactt cttcttcaga aatgaggtac acagtgcggt ccagctgttc
    34261 cacaggctga atgatttcat aatcctccag ctgctccttc catgcgtccc tctcttcctt
    34321 tgaaagctca atgggatgga tcaggccgat tactgcgtcc tcaggcagct ccagctcctc
    34381 ttcatccacc gtattaaagg agccgtcctc catataccgg aagctgcctg taagtgtttc
    34441 ttccttatat ataccccaga tcaggccaat ggcaaagggg tgcatcaccg gctttttcgt
    34501 aaacagctcc acccagtcct ccttcttcca catacgtccg gatgacaggg ccagctccag
    34561 cctctgcttc tggctggcag ccacggtttt taactgcttt ttcatagcct taaactcctc
    34621 gtaagccctt gccgccttat cttcctcatc ccgcttgccc ggagcaggca tatttttaag
    34681 ctttctgccc tcctggtccc gaacctgaat ttcaagctcc gg
//
LOCUS       DBMDUH010000007        33691 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_1035_length_33691_cov_5.438672, whole genome shotgun sequence.
ACCESSION   DBMDUH010000007 DBMDUH010000000
VERSION     DBMDUH010000007.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 33691)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 33691)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33691
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1035_length_33691_cov_5.438672"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1457)
                     /locus_tag="ACZPCG_01525"
     CDS             complement(<1..1457)
                     /locus_tag="ACZPCG_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006782624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1283783.1"
                     /translation="MTTEYKPAVGPFWAFTRICLNNWHYISRKTLTLNEEINFFTGHS
                     GSGKSTVIDALQLVLYANTDGRGFFNKAAADDSDRSLIEYLRGMVNIGDNNEFSYLRN
                     RNFSSTIVLELKRTDTEEYQCIGIVFDVETASNEISRRFFWHKGPLWENEYRTETRTM
                     SIREVERWLDTHYGKEEHFSTSHNERFRRIMYETYLGGLDSEKFPLLFKRAIPFRMNI
                     KLEDFVKEYICTEQDIHMEDMQESVMQYGRMRRKIEDTSQEIRCLTAIREAFDTFTML
                     EDKLDKYQWFVKKLELLDTRSRIRNLNDRTALGKEELNRQTEALKSAEGRIKELTDQS
                     DELLRRIASTGYGELKEQLSSLGELCEQLSRSEARWQKTTLSLKKWEEEDITSNQTLW
                     DIEEFEQRTISKETMIRLKASLASMEADTEKLRQETAARFNELNRREKQLKEELEKLQ
                     AGSKAYPKYLEEAREYLKRRLFEETGKSADVFVLA"
     gene            complement(1441..2037)
                     /locus_tag="ACZPCG_01530"
     CDS             complement(1441..2037)
                     /locus_tag="ACZPCG_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006782625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4194 domain-containing protein"
                     /protein_id="HIR1283784.1"
                     /translation="MGQTKQEITDLIRLLLRQTFLLERKYDKKTGRFQYTKEYRSCSL
                     HLEFIRRYFAVADIEVLENSHMGMIYIQGEDLVGEKLPRLATLYLLILKLIYDEQMEN
                     VSASVNVYTTLGAVHEKLGNYRLFKRQPSPSEIRRTVTLLKKYQLIEPLDLLDELSYE
                     SPFIIYPSINVVLLGDDVRELLADFEEGEEEGESDNGI"
     gene            complement(2085..3434)
                     /locus_tag="ACZPCG_01535"
     CDS             complement(2085..3434)
                     /locus_tag="ACZPCG_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009298939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wadjet anti-phage system protein JetA family
                     protein"
                     /protein_id="HIR1283785.1"
                     /translation="MKLIQEIPDRFWSLFRSVNRSAYIEALLKISEEYEYSNYFLSRE
                     MCIQLLSEYFAEKRCVIWQDELEEEEDQFEPPATRVLNWLLKTGWLRKVDDYATMTVN
                     IIIPDYAAVFIESFSRLCNEQEDEAQIYIQNIYAILFSLKHDPRSGISLLDTALVNTK
                     KLNKSLQDMLHNMDKFFGKLLEQRDYGSLLKDHLEGYVEEVVNKKYHILKTSDNFYLY
                     KTDIKSWIASMREDIPWLTRLSGREGTGEILEKLDQLEKGFDDIEHRISNMDKEHSRY
                     VKATVTRMGYLLNQEDDMKGLVIQLLNHLSKTGGTEDELKAIGERMNLSQPNVVSEAS
                     LYRRRHTRASFTEQLKDEEEMPELSAEEILNINRVKNRYSHREIEEFITSRMKDGRME
                     VTEDTVENDGDFEKLILAYDESIRRKSTYTAETEDPAPVTKGPYTYPGFVFVRRTRT"
     gene            complement(3482..5032)
                     /gene="cobA"
                     /locus_tag="ACZPCG_01540"
     CDS             complement(3482..5032)
                     /gene="cobA"
                     /locus_tag="ACZPCG_01540"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HIR1283786.1"
                     /translation="MKYTITPVSRNEKKWGTVCLVGAGPGAGELLTIRGMERLKACDA
                     VVYDHLASQELLGFVRKDCRIIYAGKEAGSHSMPQEEINRLLIGLAKEGLNVVRLKGG
                     DPFVFGRGSEEILALKEAGISWETVPGITSAVAVPELAGIPVTHRGISRSFHVITGHT
                     LAGGREQNELKAYAAMEGTLVFLMGFHSLSAITEGLIRGGKSPQTPAALIENGSLLNQ
                     RVLRADLETLAAKAKEQGFHTPAIIVVGEAAAFNLQPETVNPRGPLDGMTVGLIGTEH
                     FTDQMEIALRKEGAKTETLLRMECLEEKGKNAMASVYPRLGTYTWLVLTSVNGVRLFF
                     KGLFESGLDIRALGHMKVAAIGAATCRALLERGIRPDLVPEEYCSASLAQALFKVLHS
                     KDRVLMARAEGASVKLAQMLIKAGIPFDDIPLYAVKGVPSKRMEEQLSLCRVLIFASP
                     SGVRAFKQAGGFCRPEDSERPLYAAIGPVTAEAMKEEIGKTADIIAESYHIPGLVKAV
                     IDGTAPLP"
     gene            complement(5029..6006)
                     /gene="hemC"
                     /locus_tag="ACZPCG_01545"
     CDS             complement(5029..6006)
                     /gene="hemC"
                     /locus_tag="ACZPCG_01545"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002573114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HIR1283787.1"
                     /translation="MNHILRIGSRKSVLALRQAEMIAEQLKNKFPGLQTEILTRDTLG
                     DQLLHSPLQAFGGKGAFVSEFEQALLEGSIDLAVHSAKDMPVSLPEGLVISALSERED
                     PGDVLILPKTASKTDKKELVIGTSSPRRQLQIREQWQSICGSTKWLDKESRPVCVTLR
                     GNVQTRLKKLKEGACDGILLAAAGLKRLGIEEGEEWRFIRLSPEQFLPAGGQGILAVE
                     TKADTEAHRLCSQIDCRSSRLCLTAERSVLETLQAGCHAPVAVYANLFEEDGETKLRL
                     RGINGPEHASQLTDAELKNKIRRVDLCGTLSEISVTTLADQAGKGLLWP"
     gene            complement(6003..6665)
                     /locus_tag="ACZPCG_01550"
     CDS             complement(6003..6665)
                     /locus_tag="ACZPCG_01550"
                     /EC_number="1.3.1.76"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-2 dehydrogenase/sirohydrochlorin
                     ferrochelatase family protein"
                     /protein_id="HIR1283788.1"
                     /translation="MSYFPFFTDIEGWLCVTVGGGKVAYRKVCGLLPFGVLVHAVAKE
                     FIPEFDRLEQEEVRFRRSMRPFCLEDLKKADLVIAATNDIELNRTISRWCKQRKIPVN
                     SVQGPEDSSFLFPSLFTDGPVTAAVCTGGESPVLAHLLKERISHSIPDHMGERAGLLG
                     ALRREMKDRFPDSGGLRRDLSVRLAEAVFNMDKLPSSEEIQALLAECSQTMYIKKEEE
                     NT"
     gene            complement(6662..8122)
                     /locus_tag="ACZPCG_01555"
     CDS             complement(6662..8122)
                     /locus_tag="ACZPCG_01555"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007714994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR1283789.1"
                     /translation="MKRLCGFFLTLALSCSLTAFPVFAKPDWPADTGVQSEAGIVMDM
                     DSGAVLFAQNIHVQLPPASITKLLTALVVAEHASMDEQVTFSHDAVYNVESGSGNKLG
                     LEEGDVLSVKDSLSVMLLQSSNQAANALAEHVAGSREAFADMMNEKAEALGCRESNFE
                     NPSGLNDEAQLTSAYDMALIGAAAFSDPEILEICSAKKATLPPTKNNTEGRTYSLEHK
                     LVVTEDGTSEYYYPEAVAGKTGYTSLAGQTLVTYAVKDDRRQVAVTLKSTQKTHYSDT
                     KTILDFGFKRFKNVNVAENETHYITGEEPVEINGVSYLPSELYLDDTAVITLPNDAEF
                     GDADQYLQTEIPENHPEGAVGRIIYSYNDRQIGVAWLYTTKALSSAAEPSQEAPEQTD
                     PSSSKEPSEKKGISLPDGLIAGAGAVVLLLIAVCGIYAAVQQRKEKERLARMKERRRK
                     RLEEMGCSQDEFERLLNERKAALGKRTANDEPEDLL"
     gene            complement(8152..9033)
                     /locus_tag="ACZPCG_01560"
     CDS             complement(8152..9033)
                     /locus_tag="ACZPCG_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013656083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HIR1283790.1"
                     /translation="MQVFLNAVRGFCMALADSVPGVSGGTIAFLLGFYDEFINSLNDL
                     MGRDSKKRKKAFLFLVKLGIGWIIGFGLSVLILASLFEAHIYQISSVFFGLTLFAIPM
                     VIKEEAAVIKGQAGNLVFTLLGAALVFAISWFNPSGGEGIRVSADQLSFGLGIYIFFA
                     AMIAITAMVLPGISGSTLLLIFGLYVPVIGAIKELLHFNFSYLPVVVIFGLGVVTGII
                     AIIKFVKICLERHRSQTIYAILGLMIGSLYAILMGPTTLDVPKAPVSLSTFSIPFFIL
                     GGLILAGLELLKARLES"
     gene            complement(9145..9441)
                     /locus_tag="ACZPCG_01565"
     CDS             complement(9145..9441)
                     /locus_tag="ACZPCG_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1283791.1"
                     /translation="MLELKDFMPINFLKKEKFTGSHKGLRFRMEKLEENEETFLFVSV
                     WPEPYSYDFTPEEEKEHLKTSFDADGIARGVDWINERLTEEKERWKAALHHQTS"
     gene            complement(9451..10023)
                     /locus_tag="ACZPCG_01570"
     CDS             complement(9451..10023)
                     /locus_tag="ACZPCG_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1283792.1"
                     /translation="MMKKTYKPYKAAAVIAAAVIMTSGCGQKQQEGSTMQTEEETSAS
                     GTETSSAVQRPNPMTAVTDETAFQSLGVYMALPEEAEDPLCFILNGEVAEIQFSYHEI
                     PYSYRASNTAEDFAGIFERFTDQILTVEFKDLEGGETVSAQIRTTQSGGRFASWKWKD
                     TSYTLYTASNVSDADISSMVTMLLELISQR"
     gene            complement(10052..11308)
                     /locus_tag="ACZPCG_01575"
     CDS             complement(10052..11308)
                     /locus_tag="ACZPCG_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009298140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HIR1283793.1"
                     /translation="MKRGDTKAGMKANIRYFIKWSALALVIGSVAGAAGTIFSMGVSW
                     ATGFRLSHPSMLFFLPVSGLLIVWMYHSFHEERNRGTNMVIDAISSNERVTPATGPLI
                     FFSTILTHLGGGSSGREGAALQLGGSIGNSFGEWFKLDERDKKIAIMCGMSAVFSALF
                     GTPVAAAIFSLEVVSIGVLYYAALVPCVFSSFLAVGIARAAGLEGEHFPVEMIPALNL
                     KAMGLLVLLGILCAAVSILFCVLLHTAEHAYRKYFPDARVRILAGSFLFIALTLLSGT
                     RDYCGSSMGLIESSIEGSVRYEAFLMKMLFTAVALGAGFKGGEIVPTLCVGAALGCAF
                     GEITGFAPSLCAACGMAALFAGVTNCPITSLVIALELFGYEGMEYFSIIIAVAFALSG
                     YYGLYASQKFVYSKTRTEFINRRSNG"
     gene            complement(11329..12561)
                     /gene="ilvA"
                     /locus_tag="ACZPCG_01580"
     CDS             complement(11329..12561)
                     /gene="ilvA"
                     /locus_tag="ACZPCG_01580"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006567 - L-threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HIR1283794.1"
                     /translation="MDELTLEKFEEASEIVKQVTLETKLVYSEYFSAQTGNRVYFKPE
                     NMQYTGAYKVRGAYYKISTLSEEEKSRGLITASAGNHAQGVAYAARLAGVKATVVMPT
                     TTPLIKVNRTKGYGADVVLEGDVFDEACAHAYKLAEEHGYTFVHPFDDLDVAAGQGSI
                     AMEIIKELPTVDYILVPIGGGGLCTGVSTLAKMLNPKIKVIGVEPAGASCMKESLRQG
                     KVVTLPQVNTIADGTAVKRPGEKLFPYIQENVDEIITIDDNELIVAFLDMVENHKMIV
                     ENSGLLTVAALKHIKAEKKKVVSILSGGNMDVITLSSIVQHGLIQRDRVFTVSVLLPD
                     KPGELARVSSLLAEQHGNIIKLEHNQFISINRNAAVELRITMEAFGTEHKNQIVTALT
                     DAGYRPKLVKVKGTYSEM"
     gene            complement(12643..13578)
                     /gene="ftsY"
                     /locus_tag="ACZPCG_01585"
     CDS             complement(12643..13578)
                     /gene="ftsY"
                     /locus_tag="ACZPCG_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HIR1283795.1"
                     /translation="MAEGKKGFFGRLVEGLAKTRNNIVSGIDSIFSGFSAIDDDFYEE
                     IEETLIMGDLGIQTTMSIVEDLRNKVKEQHIKDPEQCKGILIESIKKQMNLGENAYEF
                     EHRTSVVLVIGVNGVGKTTSVGKLAGQLKDSGHKVILAAADTFRAAAIEQLTEWSERA
                     GVDIIAQQEGSDPAAVIYDAVAAAKSRKADVLICDTAGRLHNKKNLMEELKKINRIID
                     REYPDAYRETLVVLDGTTGQNALAQARQFMEVADITGIILTKLDGTAKGGIAVAIQAE
                     LGIPVKYIGIGEKIDDLQKFNADDFVNALFHVQKQ"
     gene            complement(13615..17175)
                     /gene="smc"
                     /locus_tag="ACZPCG_01590"
     CDS             complement(13615..17175)
                     /gene="smc"
                     /locus_tag="ACZPCG_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HIR1283796.1"
                     /translation="MYLKSIEIQGFKSFANKLVFEFHNGITGIVGPNGSGKSNVADAV
                     RWVLGEQRIRQLRGASMQDVIFSGTELRKPQGFAYVAITLDNSDHQLSIDYEQVTVSR
                     RLYRSGESEYMINGSACRLKDINELFYDTGIGKEGYSIIGQGQIDKILSGKPEERREL
                     FDEAAGIVKYKRRKAIAQKKLEDEQASLVRVSDILNELEKQVEPLERQSKAAREYLQL
                     KEDLKVCDANQFLLEAEHIQSQLNEISRKKQVLEGDLEETRKKGEDIRKEYDRLEAEI
                     RSLEEAVLKERSRFNEASMEKNTLEGRINVLREQIHAEERNEEHIKSRKEAITEELKG
                     KEEQLADFEAQQNRIGEQNTEELNRLNRARQEISEKENHISHLEAVIEEANAQMLRFL
                     NEKAELTARQQRYEAMSEQVNLRRSEVSQKLLRFKSDESLQEERIAEEKEHLAQVQAE
                     LVEKQVAAQEAEDGMEQADSEVRRLNRRLNDTQQEYHMAHTRLESLQNLAERYDGYGN
                     SIRRVMEVRDQIHGIHGVVADLITTGKRYETAIETALGGSIQNIVTDSEITAKQLIEY
                     LKKNKYGRATFLPLTSMGSGSAFSRPEALKEAGVIGLASELVETEECYRGLVRYLLGR
                     VVVAVNIDAAIALAKKYRYSLRIVTLEGELLSPGGSMTGGAFKNSSNLLGRRREIEEL
                     EQTCERYLKQAETIQTELSLKEAIVQEKKDEAAVLKRAGQKLALEENTLRVTISRLEE
                     KKGEIADSSSDLVREHHQLEIQAKEISESRRSLQTQKEALDSESEAAAETVNAHSLLL
                     EQAKKEKEADSEQLAQLSIKQAGLAQRLDFIRENEKRLWGEGELLKKELSQLSKGSTE
                     SGEAIAKKQEEILKIRREIEDSLLQTADSEALIAKKTEQKEAFLAQQKKLFTVREEIS
                     SRASELDKDMFRIQSQEEKLQEKLESGAAYLWSEYEMTAVQAEAMKKEDFPSLGEVSR
                     IIGGLKTRIKALGPINVNAIEDYREVSERFLFMKTQHEDLTAAQAELEKIILELDTGM
                     RRQFKEKFSEIRSEFDRVFKELFGGGRGTLELLEDEDILEAGIQIIAQPPGKKLQNMM
                     QLSGGEKALTAISLLFAIQNLKPSPFCLLDEIEAALDDSNVDRFAGYLHKLTENTQFI
                     VITHRRGTMMSADRLYGITMQEKGVSALVSVNLIEDSLTE"
     gene            complement(17166..17864)
                     /gene="rnc"
                     /locus_tag="ACZPCG_01595"
     CDS             complement(17166..17864)
                     /gene="rnc"
                     /locus_tag="ACZPCG_01595"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HIR1283797.1"
                     /translation="MNIHLKELEKKLQYEFHDKNLFSQALTHSSYANEHRLDHSRCNE
                     RLEFLGDAVLEIVTSDFLYHKYDALPEGDLTKIRASIVCEPTLAYCAGDIRLGEYLLL
                     GKGEEATGGRNRNSVVSDAMEALIGAIYLDGGFTNAKEFIHRFILNDIEHKQLFYDSK
                     TILQEMIQSRQTGVLSYEIIKEEGPDHNKSFEVRALMGEEEIGRGVGRTKKAAEQVAA
                     YNGILKLKAGESCI"
     gene            complement(17923..18156)
                     /gene="acpP"
                     /locus_tag="ACZPCG_01600"
     CDS             complement(17923..18156)
                     /gene="acpP"
                     /locus_tag="ACZPCG_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR1283798.1"
                     /translation="MEFEKLQEIIAEVLNLEPDDITMGATFVDDLGADSLDIFQIIMG
                     IEEAFDIEIPNDAAEQIVTVGDAAEQIKNALNE"
     gene            complement(18186..19205)
                     /gene="plsX"
                     /locus_tag="ACZPCG_01605"
     CDS             complement(18186..19205)
                     /gene="plsX"
                     /locus_tag="ACZPCG_01605"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HIR1283799.1"
                     /translation="MIRVAVDAMGGDNAPVEMVAGAVEAVKKKPEIQVLLVGQEKTVQ
                     EELSKYTYDKEKIQVVNASEVIATEEPPVNAIRKKKDSSIVVGMNLVKNGQADAFVSA
                     GSSGAILVGGQVIVGRIKGVERPPLAPLIPTEKGVSLLIDCGANVDARASHLVQFARM
                     GSIYMEHVVGIPNPRVAIVNIGAEEEKGNALVKETFPLLKECKDINFIGSIEAREIPH
                     GGADVIVCEAFTGNVILKLYEGVGATLIHMVKQGMMATLRSKIGALLVKPALKTTLKS
                     FDASQYGGAPLLGLKGLVVKTHGNSSRTEVCNSIIQCVTFKEQQINEKIRQSLAEEKA
                     QGPML"
     gene            complement(19359..20549)
                     /locus_tag="ACZPCG_01610"
     CDS             complement(19359..20549)
                     /locus_tag="ACZPCG_01610"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016296949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - metabolic compound salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HIR1283800.1"
                     /translation="MEKFRRIFVVVMDSLGVGEAEDAAQFGDVGSNTLGHISQSVETF
                     KIPHLRKLGMANLTPLKQVEPEEQPLGYYGKLREKSLGKDTMTGHWEMMGLEVKTPFK
                     TFTETGFPPELIKELEERTGHKVIGNKSSSGTEILDELAEEEIATGHMIVYTSADSVL
                     QICGNEETFDLEELYRCCKIARELTLKDEWKVGRVIARPYVGKKKGEFKRTSNRHDYA
                     LKPFGKTALNALKDAGFDVISIGKINDIFDGEGITKALKSKSSVHGMEQTIEVAGTDF
                     HGLCFVNLVDFDALWGHRRDPKGYAGEIERFDEKLGILLDVLREDDLLIITADHGNDP
                     TYAGTDHTREKVPFLAYSPSMKGSGALSEKDTFAVVGATAVDNFGVSMPEGTIGASVL
                     DDLV"
     gene            complement(20561..20998)
                     /locus_tag="ACZPCG_01615"
     CDS             complement(20561..20998)
                     /locus_tag="ACZPCG_01615"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HIR1283801.1"
                     /translation="MKPLEQEEIKRLIRTAKAQLDYSYAPYSHFHVGAALLAKNGNIY
                     TGCNIENAGYTPCNCAERTAFFKAVSEGVKDFDAICVTGGMNGVVTDYTAPCGVCRQV
                     MMEFCDPKEFQIIVAIDEERYIIKTLEELLPLGFGPGNLKEKQ"
     gene            complement(21018..21683)
                     /gene="deoC"
                     /locus_tag="ACZPCG_01620"
     CDS             complement(21018..21683)
                     /gene="deoC"
                     /locus_tag="ACZPCG_01620"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HIR1283802.1"
                     /translation="MGIKELMSHVDHTLLAQTATWEEIRQICDDAVAYQTASVCIPPS
                     FVKQAKEYMGDKMAVCTVIGFPNGYMTTETKEFETRNALENGADEIDMVINIGWAKAG
                     LYDEIEDEIRRLKAVCGDKILKVIIETCLLTEEEKIKMCQVVTASGADYIKTSTGFSK
                     AGATFEDIALFAAHIGENVKIKAAGGISSLADAERFLALGADRLGTSRMVKLVKNEEA
                     SGY"
     gene            complement(21739..22446)
                     /gene="deoD"
                     /locus_tag="ACZPCG_01625"
     CDS             complement(21739..22446)
                     /gene="deoD"
                     /locus_tag="ACZPCG_01625"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006770883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HIR1283803.1"
                     /translation="MSVPTPHNGAAKGDFAKTVLMPGDPLRARYIAETYLENPRQVTG
                     VRNMLGFTGTYKGKEISVMGAGMGMPSIGIYSYELFHFYDVDSIIRIGSAGALQDSLN
                     LMDVVIAMGACTDSNYAYQYGLPGTFAPIADYSLLNRAAETAKNQGTLAVVGNVLSSD
                     IFYNETADVNDKWRSMGVLAVEMEAAALYMNAARAGKKALCMLTISDHIYRQEGLSAM
                     ERQIGFGKMMEIALELA"
     gene            complement(22495..23430)
                     /locus_tag="ACZPCG_01630"
     CDS             complement(22495..23430)
                     /locus_tag="ACZPCG_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009445551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1283804.1"
                     /translation="MDVIIKNLGLLINATLIATPPLLLAALGSCFSERSGVINIGIEG
                     MMTIGAFTGAVLGLETGNGWLAFLGAGLAGALFGVIHAVVCISFHADQTIAGTAINFL
                     GPGLAVFLCKAMYNNSSDTPAMDPASKLPKLFEGVFPTGSFLSNVLNVYSVAYLVFIL
                     AGVCWFIFYKTRFGAHLRAVGEHPEACESLGIDVYRVRYSCVILSGFMAGLGGAFVTL
                     ATINQFRPSVIVGQGFIAIAAVIFGKFSPGGSMLACLLFGFCSGIKSLAGQSNMISPN
                     LISMIPYVVTLLALILFVGKAHTPAANGKIFIKSK"
     gene            complement(23432..24502)
                     /locus_tag="ACZPCG_01635"
     CDS             complement(23432..24502)
                     /locus_tag="ACZPCG_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007158270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1283805.1"
                     /translation="MNKKKSILESNLLYTLIAIVAGFIIGAVFLMIAGISPAVAYGKL
                     IDSVFGKPKYLAWVLTYASPLIFTGLSVAFSFRTGVFNIGAEGQFVVGSLVACVLGIT
                     LKLPAVIHIPLCLLAAAAAGALWSYLVGLLKVKRGIHEVLSFIMFNWIAFYLSNYVVN
                     IPAIHKEGGGEATKDVAETARILFPEAVRDVLGCKVANWGIILAVAAAVIIWVIIEKT
                     TLGYKLKAVGFNRNGALYGGINSDASVLTALSISGALAGLGGAVQILGMAGRLSQFAG
                     QEGYGFQGITVALIGSSNPIGCIFAGLFYGAMKYGGSKLSIVKAPSEVVDIIMGCVIL
                     FIAIAQIFRILFKKLAAKKEAN"
     gene            complement(24495..26039)
                     /locus_tag="ACZPCG_01640"
     CDS             complement(24495..26039)
                     /locus_tag="ACZPCG_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009213569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1283806.1"
                     /translation="MGKTSHMDESQVMVQMKDIVKKFGNFTANDHINLTVHKGEVHAI
                     LGENGAGKSTMMNVLCGLYKPTSGQIFIKGKEVHFSSPKDAIDIGIGMVHQHFMLIQP
                     FTVTDNIILGIEPVKGLSIDKAAARQKVMELSERYGMKVDPDAKIEDISVGMQQRVEI
                     LKVLYRGADTLILDEPTASLTPQEIEGLMEIIENLTRDGKSVILITHKLKEITASSDH
                     CTIIRQGKYIRTVKVDEVTENDLAAMMVGRDVNFKVDKKEQEPGGIVLEVKDVRARDY
                     RDAEILKGLNLTVRRGEIVGLAGVDGNGQTELVEILTGLKKAESGSVKMLGKEVINKT
                     PKETFESGISSIPADRQKHGLILDFSNEDNLILQHFEEAPFSNKGFLNRKAIRAHAAE
                     LIEKFDVRPRGCEEAPAGTLSGGNQQKVIIAREVTNDKDLLIAVNPTRGLDVGAIEFV
                     HKYLVEQRNKNRAVLLVSFELDEIMSLSDRIEVIFDGRITGSVPGKNADEKELGYMMA
                     GGRKNE"
     gene            complement(26057..26899)
                     /locus_tag="ACZPCG_01645"
     CDS             complement(26057..26899)
                     /locus_tag="ACZPCG_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008751693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HIR1283807.1"
                     /translation="MKAIVNRIIPFSSVDGPGNRTAIFLQGCNINCKYCHNPETRRLC
                     INCGACVGQCPAGALSLGEDNRVIWDQGACVQCDTCIHVCPNDSSPKVREMTPEEVYA
                     AVKRQIPFIRGISVSGGECMLWPDFLKALFTLAKADGLGCLIDSNGTIPLWDYPELLE
                     ISDGVMLDIKAFDDTDHRRITDEGNGIVLKNAVFLAKHGKLEEVRAVIVPDLYDGEKS
                     IRGIGTLLAPYRNIREIRIKLIAYRPMGVREAYSHYQVPSPECLTQLADILREMGFKD
                     IVII"
     gene            complement(26899..28443)
                     /locus_tag="ACZPCG_01650"
     CDS             complement(26899..28443)
                     /locus_tag="ACZPCG_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HIR1283808.1"
                     /translation="MRDISMERVNETRENILSIIKSPVLTHEQKLTNLACQADSLMEV
                     LDLPEGLDELLNVPADRKCICDLAEGHAPMRPRYIIPDYAKFMKEGSEFLQLKPPTDL
                     YEAINNLLIFYKHVPSVTNYPVYVGQLDELLEPFIDTVDEAQAKKLLGMFMTHIDRTV
                     LDSFSHANIGPRATKAGRLLLEVEAELEHAVPNITMKYEEGVTEDDFALEAIRCALHS
                     AKPSFANHKMFKGELGEGYVIASCYNGLLYGGGAYTLCRLILGNIAKRAKDIRDFKEQ
                     QLPYVLDIMARYMDERIRFEVEESGFFEHHFLAKEGFIHRDRFSGMYGLVGLAECVNI
                     LLEKEGIEGRFGHDEIATRLGVEIMDQIQAFNAVHVNPYCEGTGGRFLLHAQVGIAED
                     IDITPGTRIPIGEEPEELIDQLKVLEHFHRYFTSGTGDIFPIDMTVHRNPEYVLDIIK
                     GAFKMDLRYLSFYSSDSDVIRVTGYLVKRSEMEKLDQGQAVLQNTTGLGLGASKNGHI
                     LERKVR"
     gene            complement(28475..29584)
                     /locus_tag="ACZPCG_01655"
     CDS             complement(28475..29584)
                     /locus_tag="ACZPCG_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family lipoprotein"
                     /protein_id="HIR1283809.1"
                     /translation="MKKKTAALMLAAAMAVSLTACGGSGGTSETKAESSAAADTSAKK
                     EEAETEAAGEKKDGSEYKIGLVTDVGGVNDGSFNQSSWEGLQKAAEDFGVEVNYLESS
                     TDADYVPNIETFIDEEYDLIISVGYMLADATKQAAEANPECKFAIIDDATIDLPNVTC
                     LMFRQEQASYLVGYVAGLMTETDNIGVVIGMTNETMNLFGYGYCAGAIDANPDITIQQ
                     FNANSFADPATGKTNAGTAITNGADIVFHAAGATGLGVIEACQEAGVYAIGVDRDQYN
                     DAPGTVITSAMKRVDTAVYDSVESLLNGTLEGGLHTYDLAAGGVDIAPSQDLLPEDVK
                     KAVEDVKAKILAGEVTVPASKEEFEAAYGDVYQLD"
     gene            complement(29710..30483)
                     /gene="udp"
                     /locus_tag="ACZPCG_01660"
     CDS             complement(29710..30483)
                     /gene="udp"
                     /locus_tag="ACZPCG_01660"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015514849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HIR1283810.1"
                     /translation="MKYSEDPNKQYHIQVGKGEVGRYVILPGDPKRCEKIAKYFDDPV
                     LVADNREYVTYTGTLDGVKVSVTSTGIGGPSASIAMEELVNCGADTFIRVGTCGGMQP
                     EVKSGDAVIATGAIRMEGTSREYAPIEYPAVADLTVTNALTASAKELGLAFHTGVVQC
                     KDAFYGQHSPERMPVSYELLNKWEAWKRMGCLASEMESAALFIVAGALHVRAGSVFLV
                     VANQEREKLGLENPVVHDTDMAIRTAIGAIRELIRQEQK"
     gene            complement(30539..31291)
                     /locus_tag="ACZPCG_01665"
     CDS             complement(30539..31291)
                     /locus_tag="ACZPCG_01665"
                     /inference="COORDINATES: protein motif:HMM:NF012257.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR1283811.1"
                     /translation="MKETQKQIKKLNIETQKQIEKLNPEKVDYLKQYFAGAPRWLLEQ
                     FQVIEVPAGTVFIHEGEPAERVYVLLQGQVSAVDYRVQEAVYGFFQFYPIELFGVMEI
                     LGGMERYKTSLAAVEKSIFLRIGRDQYERWLKSDSNAFQMETKEIVNYLLEQARKERL
                     YVLLSGNQRVYQVLIKLYKSYEENGTYSAYISRKDFSEMTGLSERTITRAIKSLVSEG
                     YITKKGWNITMDSSQYQRLKALADAKMSEIDS"
     gene            complement(31486..32910)
                     /locus_tag="ACZPCG_01670"
     CDS             complement(31486..32910)
                     /locus_tag="ACZPCG_01670"
                     /inference="COORDINATES: protein motif:HMM:NF012686.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HIR1283812.1"
                     /translation="MDVRFLFVVGYLLLMVGVSLYLKRKMVKSSDDFAVAGRRLPMIV
                     LVGTLLATWCGGGGISGSAGLVYKNGPLFGILLFAGAPIGMIVLYFISGAVRKSTTYT
                     IPELFELRYGAAARNLAAICIILAYIGTTASQFKAAGNIFMITSGIDFITSTVICVIF
                     MALLALIGGMVSVAYTDALSAFMMVFGFMFGILYLSGDFGGFGGVLAQVPAEKNSIFG
                     TMNIAQVLGYVLPTLFLVLGDQNMIQRFSSAKDSRTARKSNVGLVIAEVVVCALIIGL
                     VTCAIPLYPTNDSADTILFQLAAQKLPTVLGGIVMAACMSFVITTGDSYLLSTASNLT
                     YDVWVRLIKKDADDKQKLNMLRISIVLVAFLAFVLGFYFPDILSVQMYAYTMYGATIT
                     PALICALFYKKATKAGGLAGIITGAVTTIIWDVVLRSPGGVQSAIISVPLAFIAIFLV
                     SAITQNGAKIPLEEVYKRSEESEK"
     gene            complement(32964..>33691)
                     /gene="pepT"
                     /locus_tag="ACZPCG_01675"
     CDS             complement(32964..>33691)
                     /gene="pepT"
                     /locus_tag="ACZPCG_01675"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: protein motif:HMM:NF003976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripeptide aminopeptidase PepT"
                     /protein_id="HIR1283813.1"
                     /translation="CFTPDEEIARSTENFSVERFGADLAYTMDGDALGGLVYETFNGA
                     EAQLTIHGLSVHPGTAKGIMKNAVEIGGEFMAMLPALERPQFTEGREGYFHPFVFEGT
                     VEKTYIRCLVRDHELDRYEERKAYVISCIEELNRRYGEGTVELKWDSIYYSMREVIKK
                     VPFMIDYCKQAMKECGVEPFEIAMRGGTDGSWISQMGLPCPNITAGYENAHGRFEFVS
                     IQAMAKNVEILIRLLKLYAAHKE"
BASE COUNT         8181 a         9375 c         7050 g         9085 t
ORIGIN      
        1 tcagccagca cgaatacatc tgcgcttttt cctgtttcct caaacaggcg ccgttttaaa
       61 tactctcttg cctcctccag atatttcgga taggccttgc ttcctgcctg aagcttttca
      121 agctcctcct ttaactgctt ttccctgcga tttagctcat tgaaccgggc agccgtttcc
      181 tggcggagtt tttctgtatc cgcctccata gaagcaaggg atgcctttaa gcggatcatc
      241 gtttcctttg aaatggtgcg ctgttcaaac tcctcaatat cccagagcgt ctgattggag
      301 gtgatatcct cctcctccca ttttttcagg gaaagggtgg ttttctgcca gcgggcctcg
      361 ctgcggctta actgttcgca aagctctccc agggatgaaa gctgttcctt taactccccg
      421 tatccggtag aggcaatccg gcgcagcaac tcatcgctct ggtctgtaag ctccttgatc
      481 cggccttcgg cactctttaa ggcttctgtc tgacggttca gttcttcttt tcccagagcc
      541 gtccggtcat ttaaattccg gatccgggaa cgagtatcta aaagctccag tttttttaca
      601 aaccactgat acttgtccag cttatcctcc agcatggtaa aggtatcaaa ggcttcacgg
      661 attgcggtaa ggcagcggat ctcctgactg gtatcttcaa tctttctgcg catccgtccg
      721 tactgcatta cgctttcctg catatcctcc atatggatat cctgctccgt acagatatat
      781 tccttaacga agtcctccag cttgatattc atccgaaagg ggatcgcccg tttaaaaagc
      841 agaggaaact tctcactgtc aagtcctccc agatacgtct catacatgat ccggcgaaac
      901 ctctcattat gggaggtgga aaaatgctct tcctttccat aatgggtatc cagccagcgt
      961 tccacttcac ggatggacat ggtacgggtt tctgtgcggt attcattttc ccataaaggg
     1021 cctttatgcc agaaaaagcg gcgagatatc tcattggatg ccgtttctac atcaaacaca
     1081 atgccgatac actggtattc ttccgtatcg gtgcgtttta attccagtac aatagtagaa
     1141 ctgaaattgc ggtttctcaa ataggaaaat tcgttattgt cccctatatt gaccatgccc
     1201 cttaaatatt caatgaggct tcggtccgaa tcgtcggcag ccgccttgtt aaaaaagcct
     1261 ctgccgtccg tattggcata cagcaccagc tgcagggcgt caattaccgt ggattttccg
     1321 cttccggaat gaccggtaaa aaagttgatc tcctcattta aggtaagcgt ctttctggaa
     1381 atgtaatgcc agttgttcag acaaatccgc gtaaaggccc agaacggccc gactgccggc
     1441 ttatattccg ttgtcacttt ccccttcctc ctctccttct tcaaaatcag ctaaaagctc
     1501 tctcacatca tcccccaaaa gcactacatt gatgctggga tagatgataa acgggctttc
     1561 ataagacagc tcatccaaaa gatccagagg ctcgatcagc tgatattttt tcagcagcgt
     1621 aaccgtgcgc cggatctcgc tgggagaagg ctggcgttta aaaagccggt aattccccag
     1681 tttttcatgg accgctccca gcgtagtata tacgttcacg ctggcagata cattttccat
     1741 ctgttcatca tagatcagtt tgagaataag aagataaagg gttgccagtc tgggaagctt
     1801 ttcacccacc agatcttctc cctggatata gatcatcccc atatggctgt tttccaaaac
     1861 ctcaatgtcg gcgacggcaa aataccggcg gataaattcc aggtggagac tgcagctgcg
     1921 atactccttt gtatactgga accggcctgt tttcttatca tatttgcgct ccagcagaaa
     1981 cgtctggcga agcagaaggc ggatcagatc cgtgatctcc tgtttcgtct ggcccatctg
     2041 ttcgtcatag tagggaatcc ctgcttctgt ctttataatt tccgtcacgt tctggtcctc
     2101 cttacaaata caaaacccgg ataggtgtaa ggccccttcg ttacaggcgc cggatcctcc
     2161 gtctctgccg tataggtact ttttctccgg atggattcat catatgcaag gatcagcttt
     2221 tcaaaatccc cgtcattttc caccgtatcc tccgttactt ccatccggcc gtccttcatc
     2281 cggctggtga taaattcctc aatctctctg tggctgtagc ggttctttac cctgttaatg
     2341 ttaaggatct cctctgccga aagctccggc atttcttcct catcctttag ctgctccgta
     2401 aaagatgccc tggtatgcct gcgcctgtaa agagaagcct ccgaaaccac attgggctgg
     2461 gacaggttca tacgttcccc aatggccttt agctcatcct ctgttcctcc tgttttggaa
     2521 agatggttta acagctggat caccagcccc ttcatatcat cctcctggtt taaaagatag
     2581 cccatacgcg tcacagtcgc cttaacatac cgggaatgtt ctttgtccat attggatatc
     2641 ctgtgttcaa tatcgtcaaa gcccttctcc agctgatcca gcttttcaag gatctcaccc
     2701 gtcccctctc tgccggaaag acgggtaagc cagggaatat cctcccgcat ggaagcaatc
     2761 cagcttttaa tatccgtttt ataaagataa aaattatcgc tggtctttaa aatgtggtat
     2821 tttttattta ccacctcttc cacatacccc tccagatgat cctttaaaag agaaccgtaa
     2881 tccctctgct ccaaaagctt tccgaaaaac ttatccatgt tgtgaagcat atcctgaagg
     2941 gacttattca gttttttcgt atttacaaga gccgtatcca gaaggctgat gccggatctg
     3001 gggtcatgtt ttaatgaaaa aaggatggca tagatattct ggatatagat ctgagcttca
     3061 tcctcctgct cattacaaag cctggaaaag ctttctataa aaacagccgc ataatccggg
     3121 atgataatat tgacagtcat ggttgcatag tcatccactt ttctgagcca tccggtcttt
     3181 aaaagccagt ttaaaacccg ggtggccgga ggctcaaact gatcctcttc ttcctctaat
     3241 tcatcctgcc agatcacaca gcgtttttct gcaaaatatt cacttaaaag ctgaatacac
     3301 atttccctgc ttaaaaaata attgctgtat tcgtattcct cgctgatttt taacagcgct
     3361 tcaatgtaag cagaccggtt caccgagcgg aaaaggctcc agaaccggtc cggtatctcc
     3421 tgtatcagtt tcattggttt cctcctgatt tggatacccc tattatatca ggaaaaacgc
     3481 cttatggcaa tggagcagtc ccatcaatta ccgctttcac aagtcccgga atatgataag
     3541 actcagcaat gatatccgct gtttttccga tctcctcttt catcgcctct gcagtaaccg
     3601 ggccgatggc cgcatacaag ggtctttctg aatcctccgg cctgcaaaat cctccggcct
     3661 gtttaaatgc acggacgccg gaagggcttg caaatatcag cacacggcat aacgacagct
     3721 gctcttccat tcgtttcgag ggaacgccct ttaccgcata aagaggaata tcatcaaaag
     3781 ggatccctgc ctttataagc atctgagcca gtttcacaga ggctccctct gctcttgcca
     3841 taagaacgcg gtcctttgaa tgaagcactt taaaaagcgc ctgagccagg ctggcagagc
     3901 agtattcctc cggcacaaga tccggccgga tcccccgttc caaaagcgcc ctgcaggttg
     3961 cagccccgat cgcagccacc ttcatatgcc ctaaagcccg gatatccagg ccagattcaa
     4021 aaagcccttt aaaaaacagc cggacaccgt ttacactggt aagaaccagc caggtatatg
     4081 ttcctagtct gggatatacg gaagccatgg catttttccc tttttcttcc aggcattcca
     4141 tccgaaggag cgtttctgtt ttagccccct cttttcgcag tgcgatctcc atctgatctg
     4201 taaaatgctc cgttccgatc aatccgacgg tcattccatc aagaggccct ctgggattta
     4261 ccgtttccgg ctgcaggtta aaggcagccg cctcccccac tacgataatg gcaggcgtgt
     4321 gaaagccctg ctccttcgct tttgcggcaa gcgtctctag atcagcccgc aaaaccctct
     4381 ggtttaaaag agaaccgttc tcgatcaggg cagccggtgt ctgtggagac tttcctcccc
     4441 ggatcagtcc ctctgtaata gccgacaagc tgtgaaagcc catgagaaaa acaagggttc
     4501 cctccattgc agcatatgct ttcagctcgt tctgctcacg gccgcccgcc aaggtatgac
     4561 cggtaatcac atgaaagctg cggcttatgc cccgatgggt gacgggaatc cccgcaagct
     4621 ccggtacggc taccgcagag gtgatcccag gaacggtttc ccatgaaatc ccggcctctt
     4681 ttaatgccag gatctcctcc gatcccctgc cgaacacaaa aggatcgccg ccctttaagc
     4741 ggactacatt taacccctct tttgccagcc cgatcaaaag gcggttgatc tcctcctgag
     4801 gcatggagtg gctccctgct tcctttccgg cataaatgat cctgcagtcc tttcggacaa
     4861 accctaaaag ctcctgggaa gccagatggt catagaccac ggcatcacag gcctttaacc
     4921 gctccatccc ccggatggtc aaaagttctc ccgctcccgg tccggctcct accagacaca
     4981 cggtacccca ttttttttca ttccttgata caggtgtaat ggtatatttc atggccaaag
     5041 taatcctttc cccgcttgat ctgctaatgt tgtcactgat atttctgaaa gtgttccgca
     5101 caggtcaacc cggcgtatct tatttttaag ctccgcgtcc gtaagctgtg aagcatgttc
     5161 cggtccattg atcccgcgaa ggcgcagttt cgtttctcca tcttcttcaa agaggttcgc
     5221 atatacggcc accggcgcat gacagcctgc ctgaagcgtt tcaagcactg acctttctgc
     5281 tgtaaggcac agacgggatg atctgcagtc gatctggctg cagagcctgt gagcctctgt
     5341 atccgctttc gtctccactg caaggattcc ctgtcccccg gcaggcagaa actgttccgg
     5401 actcaagcgg ataaaacgcc attcttcccc ctcttcaatt cccaggcgct ttaaccctgc
     5461 tgctgctaaa aggatcccgt cacaggcacc ctctttcagt ttttttaagc gggtctgcac
     5521 atttccccga agcgtcacac acacaggccg gctttcctta tccagccatt ttgtactgcc
     5581 gcagatactc tgccactgct cccggatctg cagctggcgt ctgggactgg aggttccgat
     5641 gacaagctcc tttttgtctg tctttgaggc agtctttggc agtatcagta catcccccgg
     5701 atcttcgcgc tcagacagcg ctgaaatcac cagcccctct ggaagagaaa ccggcatatc
     5761 cttggcacta tggactgcaa ggtcaatgga gccttctaaa agagcctgtt caaattcgga
     5821 aacaaatgct cccttccccc caaatgcctg gagaggagaa tgaaggagct ggtctcctag
     5881 cgtatccctt gtaagaatct ctgtctgaag acccggaaac ttatttttca gctgttcagc
     5941 gatcatttct gcctggcgca gagccagaac acttttccgg cttccgatac gaagtatatg
     6001 gttcatgtgt tttcttcctc tttttttata tacatggtct gagaacattc agccagcaat
     6061 gcctgtattt cctctgatga agggagctta tccatattaa aaactgcctc tgcaagccgg
     6121 acggaaaggt cacgccgcag cccgcctgaa tctggaaaac gatccttcat ctctctgcgc
     6181 agcgctccta aaagcccggc ccgttctccc atatggtccg gtatggaatg ggaaatgcgc
     6241 tcctttaaaa gatgggccag cacagggctt tcaccccctg tacatacggc agctgtcacc
     6301 gggccgtccg taaacagcga cgggaaaaga aagctgctgt cttctggccc ctggaccgaa
     6361 ttaaccggaa ttttcctttg tttgcaccac cgggaaatgg tccggttcag ctcaatatca
     6421 ttagtggcag caatgaccag atctgctttc tttaaatcct ccaggcaaaa ggggcgcatg
     6481 ctccgcctga aacgcacttc ctcctgttca aggcggtcaa actcagggat aaactccttt
     6541 gccaccgcat ggaccaaaac tccaaagggg agaaggccgc agaccttacg gtatgcgacc
     6601 ttcccccctc cgactgtcac acagagccac ccctcaatat ctgtaaaaaa agggaaataa
     6661 ctcataacag atcctccggt tcatcattgg ctgtccgttt tcccaaagca gcctttctct
     6721 catttaacag acgttcaaac tcatcctggc tgcagcccat ttcctccaga cgctttctgc
     6781 gcctttcctt catccgtgcc agacgttcct tctcttttct ttgctgaacc gcagcgtaaa
     6841 tcccgcaaac cgcaatcaga agcaggacaa ctgcgcccgc tccggcgatg agcccgtcgg
     6901 gtaaagaaat ccctttcttt tcagacggct ctttggatga agacggatct gtctgttcag
     6961 gtgcctcctg tgaaggctct gccgcactgc ttaaagcctt tgtggtatac agccatgcca
     7021 ctccgatctg tctgtcatta taggaataga tgatccggcc gacagccccc tctggatggt
     7081 tctctggaat ctcagtctgc agatactgat ctgcgtctcc aaattctgca tcattgggaa
     7141 gagtgatcac cgctgtatca tccagataca gctcggatgg cagataggat acgccgttta
     7201 tttctaccgg ctcttcaccg gtgatgtaat gggtttcatt ttctgccaca tttacatttt
     7261 tgaaccgttt aaaaccaaaa tccagaatgg tctttgtatc tgaataatgg gtcttttggg
     7321 tactctttaa agtcaccgct acctggcgcc ggtcatcctt taccgcgtaa gttacaaggg
     7381 tctgtcctgc aagagaggta tagcctgtct ttcctgccac tgcttccgga taatagtatt
     7441 cgctggttcc gtcctctgta accaccagtt tatgctcaag ggaataggta cgcccttctg
     7501 tattgttttt ggtgggagga agcgtggctt tttttgccga acagatttca aggatctccg
     7561 gatctgaaaa tgctgccgcg ccgatcaggg ccatatcgta ggcagaggtc agctgggcct
     7621 catcatttaa gccggacggg ttttcaaaat tgctttcccg gcagcctaat gcctctgcct
     7681 tttcattcat catgtccgca aaagcctctc ggctccctgc cacatgttct gccagggcgt
     7741 tggctgcctg attggaggac tgcaaaagca taacagacag actgtccttc acagacagaa
     7801 catccccctc ttccaggccc agcttatttc cgcttcccga ttctacatta taaacggcat
     7861 catgagaaaa cgtaacctgc tcgtccatgg aagcatgttc agccactaca agagctgtga
     7921 gcagttttgt aatactggct ggcggaagct ggacatggat attctgggca aacagcactg
     7981 ctcctgaatc catatccatc acaatcccag cctcggactg gacaccggta tccgcaggcc
     8041 agtccggttt tgcgaacacg ggaaaagctg tcagggaaca gctgagcgca agtgtcaaaa
     8101 agaaaccgca caggcgtttc atccgtcgga tcatcgtata tacccccaat attatgattc
     8161 aagccttgcc tttaaaagct ccagaccagc cagaatcagc ccgcccaaaa taaagaacgg
     8221 gatactgaag gtagaaaggc ttacaggagc ttttggcaca tccagcgtag tgggccccat
     8281 aagaatggca tatagggagc cgatcatcag gcccagaatg gcgtagatcg tctgggaacg
     8341 gtggcgttcc agacatattt ttacaaattt tataatcgca ataattcctg tgacaacacc
     8401 caggccgaag atgaccacca cgggcagata actgaaatta aaatggagca gctccttgat
     8461 cgccccgata acgggaacat agagtccaaa gatcaaaaga agggtagagc cggaaattcc
     8521 cggaagtacc atggctgtaa ttgcgatcat agcagcaaag aaaatataaa tgcccaatcc
     8581 gaatgaaagc tgatccgctg atacacggat gccctctccg ccggatggat taaaccagga
     8641 aatggcaaat accagagctg cgcccaaaag ggtaaagacc agatttccag cctgtccttt
     8701 tatcaccgcc gcttcttcct ttataaccat cggtatagca aacaaggtaa ggccaaagaa
     8761 cacagagctg atctgataaa tgtgagcttc aaacagactg gcaagaatta aaacggacag
     8821 accaaaacca atgatccagc caatccccag ctttaccaga aaaagaaacg cctttttgcg
     8881 ttttttgctg tcacgcccca ttaaatcatt taaagaattg ataaattcat cataaaaccc
     8941 aagcaaaaac gcaatggtcc cgccggaaac tccgggtacg ctgtctgcca gagccatgca
     9001 aaatcctctc actgcattta aaaacacctg catcttcctc ctgtttcgtt cgaaaatttc
     9061 aaatcggacc gctctggcta tactaccacg gcaatccgca cattgaatta ttatacatga
     9121 aataggagga aattcaagta aatcttatga ggtctgatga tgaagcgccg ctttccaccg
     9181 ctccttttct tctgtcagac gttcgttgat ccagtcaacg ccgcgggcga tcccgtccgc
     9241 atcaaaggag gtttttaaat gctccttttc ctcttccggt gtaaaatcat agctgtaggg
     9301 ttccggccac acacttacga aaagaaaggt ttcctcattc tcctccagtt tttccatccg
     9361 aaaccgcagt cctttatgac taccggtaaa tttttccttt tttaggaaat ttatcggcat
     9421 aaaatccttt aactccagca tagccttctc ctaccgctga gagatcagct ctaataacat
     9481 agttaccatt gatgaaatat ccgcatccga tacgttcgat gccgtataga gcgtatatga
     9541 cgtatccttc catttccagg aagcaaagcg gcctccactc tgggtagtac ggatctgggc
     9601 agaaaccgtt tctccccctt ccaaatcctt aaattcaacg gtcaggatct gatccgtaaa
     9661 gcgttcaaaa atcccggcaa aatcctctgc cgtgttggaa gcacggtagg aatagggaat
     9721 ttcatgatat gaaaactgaa tttcagctac ctctccattc aggataaagc agagaggatc
     9781 ttctgcctcc tccggaagag ccatataaac cccaagagac tgaaaggcag tttcatctgt
     9841 taccgcagtc atgggattag gtctctgcac ggcagacgag gtttccgtac cggatgcaga
     9901 cgtctcctct tctgtctgca tcgtacttcc ctcctgctgt ttttgtccgc atccggaggt
     9961 cataataacg gcagccgcta taaccgccgc cgctttatat ggtttatacg tttttttcat
    10021 catactcact cctttttaag ccgtacctat cctatccgtt ggatctgcga ttgataaatt
    10081 ctgtccttgt tttggaataa acaaacttct ggctggcata caggccataa tagcccgaca
    10141 gggcaaatgc taccgcaata ataatagaga aatactccat cccttcataa ccaaaaagct
    10201 ccagagcaat cactaaggat gtgataggac agttagtcac tccggcaaaa agagccgcca
    10261 tgccgcaggc cgcacagaga gagggagcaa accccgtaat ctccccaaac gcacagccta
    10321 acgctgctcc cacgcagagg gtaggcacaa tctctcctcc cttaaatcct gctcctaaag
    10381 caactgctgt aaagagcatc ttcataagaa aagcctcata gcgaacactt ccctctatgc
    10441 tgctttcgat cagtcccata ctgctgccgc agtaatctct ggttcctgat aaaagggtaa
    10501 gggcgataaa aaggaagctg ccagccagga tccgcactct ggcgtctgga aaatacttcc
    10561 ggtaagcatg ttctgccgta tgaagcagaa cacagaacaa gatgcttacc gcagcacaga
    10621 gtatacctaa aagtaccaga agccccattg ctttcaaatt cagcgccggt atcatttcca
    10681 ccgggaaatg ttctccttca agtccggccg cccttgcgat ccccaccgcc agaaaggagg
    10741 aaaatacgca gggaaccagg gctgcatagt agaggacacc aatgctgact acctccaggg
    10801 aaaaaatcgc cgctgccacc ggcgttccaa aaagagcgga gaatactgcg ctcatgccgc
    10861 acatgatcgc aatcttttta tcccgctcat ccagtttaaa ccactcccca aaggaatttc
    10921 cgatgctgcc tcccagctga agagccgccc cctcacgacc agaagagcca ccccccagat
    10981 gagttaagat agtagaaaaa aagattaaag gtcctgtggc aggcgtcacc cgttcattgg
    11041 aggaaattgc atcaataacc atattggttc ccctgtttct ttcctcatgg aaggagtggt
    11101 acatccagac gatcaaaagg ccggacaccg gaagaaaaaa cagcatggac ggatgggaca
    11161 gccgaaaacc ggtggcccag gaaactccca tggaaaaaat cgtgcctgcc gctccagcca
    11221 cagaaccgat gaccagagca agggcactcc atttgataaa gtaacggata ttggccttca
    11281 ttccggcttt ggtatcccca cgtttcattt aatacacccc cacatgccct acatctcgct
    11341 gtaggtaccc tttaccttca caagcttggg gcggtatcct gcatctgtca aagccgttac
    11401 gatctgattt ttatgctctg tcccaaatgc ctccatggta atccgaagtt ccacagccgc
    11461 attgcggttg atgctgataa actggttgtg ctccagcttg ataatattgc catgctgctc
    11521 tgccaaaaga gaagataccc ttgcaagctc tcccggctta tccggaagca gcacggatac
    11581 ggtaaagact ctgtctctct ggatcaggcc atgctgtacg atagaggata aggtaatcac
    11641 atccatattt ccaccactta agatagaaac aaccttcttc ttttccgcct tgatgtgctt
    11701 tagggcagct acggttaaaa ggccggaatt ttctacgatc atcttgtggt tttccaccat
    11761 atccaaaaag gctacgatca gctcattatc atcaatggtg atgatctcat ctacattttc
    11821 ctggatatag gggaacagct tttcacccgg acgcttaaca gccgtaccat ctgcaatggt
    11881 attgacctga ggaagggtta caacttttcc ctggcgcagc gattccttca tacagctggc
    11941 tccggccggc tcaacgccga ttactttgat ctttggattt aacatcttgg caagggtaga
    12001 tactcctgtg cacaggccgc cgcctccgat aggaaccagg atataatcaa ctgtaggaag
    12061 ctctttgata atctccatgg caatagaacc ctgtcctgct gcaacgtcca gatcatcaaa
    12121 aggatgtaca aaggtgtatc cgtgctcctc tgccagtttg tatgcgtgag cgcaggcttc
    12181 gtcaaataca tctccttcca gaaccacatc ggctccgtag cctttggtac ggttaacctt
    12241 gatcaaaggc gttgtggtag gcattactac ggttgccttt accccggcca gacgcgcggc
    12301 ataggccacg ccctgagcat ggtttccggc agaggcagtg atcagtcctc tggacttttc
    12361 ttcttcggac agggtactga tcttatagta cgcgcccctt accttgtagg ctcctgtata
    12421 ctgcatattt tccggcttaa aatacaccct gtttcctgtc tgtgcagaaa aatattcact
    12481 gtacaccagc tttgtctcca gtgttacctg ttttacgatc tcagaagctt cctcaaattt
    12541 ctctaatgta agttcatcca tactcgttct ctccttggca tttattggtt acaactatat
    12601 cactttattg tgataaattc aacagaaaac cgaaagagga tcctactgtt tctgtacatg
    12661 gaacagggcg tttacaaagt catccgcatt aaacttctgc agatcgtcaa tcttttcacc
    12721 gattccgatg tattttacag gaattcccag ttccgcctgg atcgctacgg caattcctcc
    12781 cttggccgtt ccatccagct ttgtaaggat gatgcccgtg atatcggcca cctccataaa
    12841 ctgtctggcc tgagccaggg cgttctgtcc ggtggttccg tctaagacca ccagcgtctc
    12901 cctgtatgca tcaggatact cccggtcaat gatccggttg atctttttaa gctcttccat
    12961 caggttcttt ttattgtgaa gccgtcctgc agtatcacag atcagcacat ctgcctttct
    13021 ggatttggct gcagccaccg catcataaat gactgccgcg ggatcggagc cctcctgctg
    13081 ggcgataata tccactccgg cccgctccga ccactcggta agctgctcaa tggctgcggc
    13141 gcggaaggta tcggccgcag caaggattac cttatgacca ctgtccttta actggccggc
    13201 cagctttcct acagaggtgg tttttcccac gccgtttacg ccgatcacca gcacaaccga
    13261 ggttctgtgc tcaaattcgt aggcattttc gcccagattc atctgctttt tgatgctttc
    13321 gatcagaatc cccttgcact gctccggatc ctttatatgt tgttccttta ccttatttct
    13381 caggtcctct acaatggaca tggtggtctg gatccccaga tctcccatga tcaaagtctc
    13441 ctcgatctcc tcataaaaat catcatcaat ggcagaaaaa ccgctgaaaa tagaatcaat
    13501 tcctgataca atgttgttcc gtgttttggc aagtccttct accagacgcc cgaaaaatcc
    13561 cttttttcct tcagccataa ggcttatcct cctttttata tacggacaga aaaactactc
    13621 tgtcagactg tcctcgatca aatttacact caccagggca gatacgccct tttcctgcat
    13681 ggtaatgccg taaaggcggt cggcagacat catggtaccc cttcggtggg taatgacaat
    13741 aaactgtgta ttttctgtaa gcttatggag atacccggca aaccggtcca cattggaatc
    13801 atccagagcc gcctcgatct catctaacag gcagaagggg gacggcttta aattctggat
    13861 ggcaaacagc agggaaatgg cagtcagcgc tttttcccct ccggaaagct gcatcatatt
    13921 ctgcagcttt ttgcccggcg gctgggcgat aatctgaata cctgcctcta aaatatcctc
    13981 atcctccaaa agctccagag tcccccgtcc tccgccgaag agttccttaa acacccggtc
    14041 aaactcagag cggatctctg aaaatttttc cttaaactgg cggcgcattc ccgtatccag
    14101 ctccaggatg atcttttcta actccgcctg ggcagcggtc agatcctcat gctgggtttt
    14161 cataaagagg aaacgctcgg atacctccct gtaatcctca atggcattga cattgatcgg
    14221 gccaagtgcc ttgatccggg tttttaatcc gcctatgatc cggcttactt ctcccagaga
    14281 aggaaaatcc tcctttttca tcgcctctgc ctgaacagcc gtcatctcat attccgacca
    14341 cagatatgcg gcccctgatt ccagcttttc ctgcagcttc tcctcctggg actgaatgcg
    14401 gaacatatcc ttatccagtt ccgaagcgcg ggatgaaatt tcttcccgga ctgtaaaaag
    14461 cttcttttgc tgtgcgagaa acgcttcctt ctgctctgtt ttttttgcga tcaacgcttc
    14521 gctgtctgcc gtctgaagca gggaatcctc aatctctctg cggattttta aaatctcctc
    14581 ctgctttttt gcaatggctt ctcctgattc tgtgcttcct ttagagagct gggacagttc
    14641 ctttttaagc agctcccctt ctccccaaag acgtttttca ttctcacgga taaaatccag
    14701 cctctgggcc agtcctgcct gcttaatgct taactgcgcc agctgctcgc tgtctgcctc
    14761 tttttccttc tttgcctgtt ccaaaagaag gctgtgtgca tttacagtct cagcagcagc
    14821 ctcactctcg ctgtccagag cctctttctg tgtttgcagg cttctgcggc tttcgctgat
    14881 ctcctttgcc tgaatctcca gctgatgatg ctcccttacc agatcagagg aagaatcggc
    14941 aatctctccc tttttctcct ccagtctgga aatagtaacc cgaagcgtat tctcttccag
    15001 cgccagcttc tgtccggctc tttttaagac agccgcctca tccttttttt cctgaacaat
    15061 tgcctcttta agacttaact ctgtctgaat ggtctccgcc tgcttcaagt aacgttcgca
    15121 ggtctgttcc agctcctcga tctccctgcg gcgccctaaa agattactgc tgtttttaaa
    15181 cgcgcctccc gtcatggaac cgcctgggct taaaagctca ccctccaaag tgacaattct
    15241 caggctgtag cggtattttt tagctaaggc aatcgctgca tcaatgttaa cagccactac
    15301 cacccggccc aaaagatacc gtaccagtcc ccgatagcat tcttctgttt ctaccagttc
    15361 gcttgcaagg ccgatcacgc ctgcttcctt taacgcctca ggccttgaaa aggccgatcc
    15421 gcttcccata ctggtaagag gcagaaatgt agcccggcca tatttatttt tctttaaata
    15481 ttcgatcagc tgcttggccg taatctctga gtctgtgaca atgttctgga tactgccccc
    15541 aagggctgtt tctatggctg tttcgtacct tttgccagta gtgatcaaat ctgccaccac
    15601 gccgtggatc ccatggatct ggtcccggac ctccatcacc cggcggatgc tgtttccgta
    15661 gccgtcatag cgctccgcca gattctgcag cgattccagc ctggtatggg ccatgtggta
    15721 ttcctgctgg gtatcattta accgacggtt taaccggcgg acctcgctgt ctgcctgctc
    15781 cataccatcc tcggcttcct gggctgcaac ctgcttttcc accagctccg cctgtacctg
    15841 tgcaagatgc tccttttcct ctgcgatcct ctcctcctga agagattcgt cacttttaaa
    15901 ccggagaagc ttctgggaaa cctcgctgcg gcgcagattt acctgctccg acatagcctc
    15961 atagcgctgc tgtctggccg tcagctcggc cttttcattc aggaagcgga gcatctgagc
    16021 gtttgcctcc tcgatcaccg cttccagatg gctgatatga ttttcttttt cagaaatctc
    16081 ctgtctggca cggttcagcc ggtttaattc ctctgtattc tgctccccaa ttcggttttg
    16141 ttgcgcttca aagtccgcaa gctgttcctc ttttcccttc agttcctcag tgatggcttc
    16201 ctttcggctt ttaatatgct cctcattccg ttcttctgcg tggatctgtt ctcttaaaac
    16261 attgatccgg ccttccagcg tgtttttttc catggacgcc tcattgaaac ggctccgttc
    16321 ttttaaaaca gcttcctcca gactgcggat ttctgcttcc aggcggtcat attcctttcg
    16381 gatgtcctca cctttttttc tggtctcctc cagatctcct tccaaaacct gctttttcct
    16441 ggaaatttcg tttaactggc tctggatgtg ctctgcttcc aacagaaact ggttggcatc
    16501 acagaccttc agatcttcct ttaactgcag atactccctg gctgcctttg actgacgctc
    16561 taagggttcc acctgctttt ccagctcatt taaaatatcc gatacacgta ccagactggc
    16621 ctgttcgtcc tccagctttt tctgtgcaat ggccttgcgc cgtttgtatt tcacaatacc
    16681 tgcagcttcg tcaaaaagct cccgccgttc ctccggcttt ccgcttaaga tcttatcgat
    16741 ctgtccctgt ccgataatgg aatagccctc ttttccgatt cccgtatcat aaaagagttc
    16801 attgatatcc ttcagacggc aggcgctccc atttatcata tattcacttt caccggagcg
    16861 gtaaaggcgt ctggataccg tcacctgctc ataatcgatg gacagctggt gatcgctgtt
    16921 atccagggtg atcgccacat atgcaaaacc ctgaggcttt cttagctcgg ttccggaaaa
    16981 gatcacatcc tgcatactgg ctccgcgcag ctggcgtatc ctctgttcgc ctaacaccca
    17041 gcgcactgca tctgctacat tgctctttcc gcttccgttg ggtcctacaa tacccgtaat
    17101 gccgttatga aattcaaaga ccagtttatt ggcgaaggat ttaaagccct ggatctcgat
    17161 acttttcaga tacatgattc gcccgcctta agctttaaaa taccgttgta ggcggctacc
    17221 tgttccgccg ccttttttgt gcgtcccacg ccccggccga tctcttcctc tcccatgagc
    17281 gcccggacct caaaggattt attgtgatca ggcccctcct cctttatgat ttcataggac
    17341 agaacaccgg tctgccggct ctggatcatc tcctgtaaga tagtcttgct atcgtaaaag
    17401 agctgcttgt gttcgatatc atttaaaata aatctgtgta taaactcttt tgcattagta
    17461 aaaccaccgt ccagataaat cgctccgatc agggcttcca tcgcatccga taccacagaa
    17521 ttgcggtttc gccctccagt tgcctcttct ccctttccaa ggagcaggta ctcgcccagc
    17581 ctgatatctc ctgcacagta tgccaaagta ggttcgcaga caatacttgc acgtattttt
    17641 gtcaagtctc cctccggaag cgcatcgtat ttatggtata aaaagtcact ggtaacaatt
    17701 tcaagcactg catcgcctaa aaactccagg cgttcattgc agcggctgtg atccagccgg
    17761 tgttcattcg cataggagct gtgggtcagc gcctggctga agagattttt atcgtgaaat
    17821 tcatactgaa gctttttttc cagctctttt aaatggatat tcatatgtat attccttcct
    17881 ttttaaacgc acaaaagggc tgtaaaaaca gccccgcgcg ttttattcat tcaatgcgtt
    17941 tttgatctgc tctgcagcgt ctccgaccgt aacgatctgt tctgcagcat cattggggat
    18001 ttcgatatcg aacgcctcct caattcccat aatgatctgg aaaatatcca gtgaatcggc
    18061 tccaagatcg tctacaaagg tagctcccat ggtgatgtca tccggctcca gatttaaaac
    18121 ctctgcaata atttcctgca atttttcaaa ttccatattc tatcccctct cctgaatctg
    18181 tttctttata acataggccc ctgagccttt tcctctgcca gcgactggcg gatcttttcg
    18241 ttgatctgct gctccttaaa ggtcacgcac tggataatgg aattgcacac ctcagtccgg
    18301 ctggaatttc catgtgtctt cactaccagt ccctttaatc ccaaaagagg cgcgccgccg
    18361 tactgactgg catcaaaaga ttttaaggtg gtctttaaag ccggttttac aagaagtgcc
    18421 ccgatcttgc tccggagcgt tgccatcatc ccctgcttta ccatatggat caaagtagct
    18481 cctacaccct catacagctt taaaatcaca tttcctgtaa aggcctcaca gacaattacg
    18541 tctgcgccgc cgtgggggat ttctctggcc tcaatacttc cgatgaaatt gatatcctta
    18601 cattccttta acagcgggaa cgtctccttc accagtgcgt ttcccttttc ctcttccgca
    18661 ccgatattta caatggcaac tcttggattt ggaattccta ctacgtgttc catatagatc
    18721 gaacccattc tggcaaactg taccagatga gacgccctgg cgtctacatt cgccccgcag
    18781 tcgatcagaa gggatacgcc tttttctgta ggaataagcg gtgcaagagg cgggcgctcc
    18841 acgcccttga tccgtcccac gatgacctgt ccgcctacta agatcgctcc tgagcttccg
    18901 gccgatacaa aagcgtctgc ctgtccgttc tttaccaggt tcatgcctac tacgatggat
    18961 gagtctttct ttttccggat cgcatttacc ggcggctctt ctgtagcaat cacctctgag
    19021 gcatttacca cctggatttt ctctttgtca taggtatatt tggaaagttc ttcctgtacg
    19081 gtcttttcct gtcctaccaa aagaacctgg atctccggtt tcttctttac cgcctcaacg
    19141 gctcctgcaa ccatctccac aggcgcattg tctccgccca tagcatctac cgctactctg
    19201 atcataaaaa agactctcct ttcgtctttg cgatacaatt tataatatac tagatatccg
    19261 tctataaatc aatacgtttt tatagctgca ccagggtaaa aaacgccgaa aaaaggaccg
    19321 tccccattca gggacagccc tttaaaaaac agataaaact ataccagatc gtcaagaacc
    19381 gaagctccga tggtgccctc cggcatggaa acaccaaaat tatcaactgc tgtagcgccg
    19441 accactgcaa aggtatcctt ttcgctcagc gcgccgcttc ccttcatgga aggggaatag
    19501 gccaaaaacg gtaccttttc acgggtatga tccgttcctg cataggtggg atcattgccg
    19561 tgatctgccg tgatgatcaa aagatcatct tctcttaata cgtcaagaag gatccccaac
    19621 ttctcatcaa agcgttcaat ctcccctgca tatccctttg gatcccgtct gtggccccag
    19681 agggcgtcaa aatccaccag attcacaaag caaagaccat gaaaatccgt tcccgccact
    19741 tctatggtct gctccatgcc atgaacagag cttttcgatt tcagggcctt tgtaatgcct
    19801 tctccgtcaa agatatcatt gatctttcca atggaaatca catcaaagcc tgcatccttt
    19861 aaagcattta atgctgtctt cccaaagggc tttaaggcat agtcatggcg gttgctggtg
    19921 cgcttaaatt cgcccttttt ctttcccaca taaggccttg caatgacgcg ccctaccttc
    19981 cattcatctt ttaacgtaag ctctctggca atcttacagc accggtacag ctcctcaaga
    20041 tcaaaggttt cctcattgcc gcagatctgc agcacagagt cggcagaggt atacacgatc
    20101 atatggccgg tggcaatctc ctcctctgcc agctcgtcca ggatttccgt tccgctggag
    20161 cttttatttc caattacctt atgaccggtg cgctcctcca actccttgat cagctccggc
    20221 ggaaagcccg tctccgtaaa ggtcttaaag ggcgtcttca cctccagccc catcatctcc
    20281 cagtggccgg tcatggtatc cttacccagg ctcttttcac gcaactttcc ataatagcca
    20341 agaggctgtt cctccggctc cacctgtttt aaaggtgtga gatttgccat cccaagcttt
    20401 cttaaatgag ggatcttaaa ggtttctacc gactgagaga tgtggcccag ggtattggaa
    20461 cctacatctc caaactgggc agcgtcctct gcctctccta cccccagaga atccattaca
    20521 accacaaaga tacgtctgaa tttttccatg ttctgctcct ttactgcttt tctttcagat
    20581 ttccgggacc aaagcccagg ggaagaagct cttccagcgt ttttatgata taccgttctt
    20641 catcaattgc cacgataatc tgaaactcct ttggatcaca gaactccatc attacctggc
    20701 ggcatacgcc gcagggggct gtatagtctg taacaacgcc gttcatccct ccggtaacgc
    20761 agattgcatc aaaatccttt actccctcgc tgactgcctt aaaaaatgcc gtccgctctg
    20821 cacagttaca gggagtatag ccggcatttt caatattgca gccagtatat atgttcccgt
    20881 tttttgcaag cagagccgcg cccacatgaa aatgggaata cggtgcatag gaataatcca
    20941 actgagcctt cgcagtacgg atcagacgtt ttatctcttc ctgttccagt ggtttcatcg
    21001 tctactcctt cctggcatca ataaccggat gcttcttcat tttttaccag ctttaccata
    21061 cggctcgttc ccaggcggtc tgctcccagc gcaaggaaac gctctgcatc cgccagagag
    21121 gaaatgccgc ctgccgcctt gatctttaca ttttctccta tatgagcggc aaaaagcgca
    21181 atgtcctcaa aggtggcgcc tgcctttgaa aagcccgtag aggttttaat ataatcggcg
    21241 ccggaagcag tgaccacctg gcacatcttt atcttttctt cctctgtcag cagacaggtt
    21301 tcaatgataa cctttaagat cttatccccg cacactgcct tcagcctgcg gatctcatcc
    21361 tcaatttcat cataaaggcc tgcctttgcc cagccgatat tgatcaccat gtcaatctcg
    21421 tctgctccgt tttccaaagc atttctggtt tcaaactcct ttgtttccgt agtcatatag
    21481 ccgttaggaa aaccgatcac cgtgcagacg gccatcttat ctcccatata ttcctttgcc
    21541 tgcttaacaa agcttggcgg aatgcagaca gaagctgtct gatacgcgac ggcgtcatca
    21601 cagatctgcc ggatctcctc ccatgtagcc gtctgtgcca gcagtgtatg atctacatgg
    21661 ctcataagtt cttttattcc cattttcata tcctccttta aacatatatt catctttcct
    21721 tcaccggatc cataagaatc aggccagttc cagggcaatc tccatcatct ttccaaaacc
    21781 gatctggcgc tccattgcgc ttaatccctc ctggcgatag atatgatcgg aaatagtaag
    21841 catacaaagc gcctttttgc ctgccctggc agcattcata tacagggctg ccgcttccat
    21901 ttccactgcc agaacaccca tagaacgcca tttgtcattc acatctgcag tttcgttata
    21961 aaaaatatca gaggaaagga catttcccac cacagccagc gttccctgat tctttgcagt
    22021 ttcggcagcc ctgtttaaaa ggctgtaatc tgcaatgggc gcaaatgtac ccggaagtcc
    22081 gtactgatac gcataattgg aatccgtaca ggcgcccatg gcaataacca catccatcag
    22141 attcaaagaa tcctgaagtg cgccagcaga tccgatgcgg ataatactgt ccacatcgta
    22201 aaaatgaaac agctcatagg aatagatacc gatggagggc attcccatgc cggctcccat
    22261 gactgaaatc tcttttccct tataggttcc cgtaaatccc agcatattgc ggactcccgt
    22321 gacctgtctg ggattctcaa gataggtttc agcgatatat ctggcccgaa gaggatctcc
    22381 gggcataagc acggttttgg caaaatctcc ttttgccgcg ccgttatgag gagttggtac
    22441 tgacatagat aagcttcctt tccaaaccgc atctgtaagg cagcggttta cgttttactt
    22501 ggatttgata aaaatctttc cgttggcagc cggcgtatgg gcttttccaa caaagaggat
    22561 cagcgccagc agggttacca cataggggat catggaaatc aggttcggcg agatcatgtt
    22621 gctctggccc gcaaggctct tgatgccgct gcagaagcca aagagaaggc aggcaagcat
    22681 agagccaccc ggtgaaaact taccgaagat aacggccgca atggcgataa agccctgtcc
    22741 tacaattacg cttgggcgga actggttaat ggtagccagc gttacaaaag cgcctcccag
    22801 gccggccata aagccggaca gtatcacaca gctgtaacgc acgcggtaca cgtcaattcc
    22861 cagagattca caggcctccg gatgctctcc cactgcccgc agatgggcgc cgaagcgcgt
    22921 tttgtaaaag ataaaccagc atacgcctgc caggataaac accagatatg ccactgaata
    22981 tacattcagt acattgctta aaaaggagcc tgttggaaat acgccctcaa aaagctttgg
    23041 aagcttgctt gccggatcca tggccggagt atcggaggaa ttattgtaca tagctttgca
    23101 gaggaatacc gcaagacctg ggcctaaaaa gttgatggca gttccggcaa tggtctgatc
    23161 tgcatgaaag gaaatacata cgaccgcatg aatgacgcca aaaagcgcac ccgcaagacc
    23221 cgctcccaaa aaggccagcc agccgtttcc cgtttccagc ccaagtaccg caccggtaaa
    23281 tgcgccgatg gtcatcatgc cctcaattcc aatattgata actccgctcc tctcagaaaa
    23341 gcaggagccc agggccgcca gaagaagggg cggcgttgcg atcaaagttg cattgatcaa
    23401 aaggccaaga tttttaatga ttacatccat attagtttgc ctccttcttt gcagcgagct
    23461 tcttaaacag gatacggaag atctgggcaa tggcaataaa gaggatcaca cagcccatga
    23521 taatgtctac cacctctgaa ggcgctttta caatgctcag cttggagccg ccgtacttca
    23581 tggctccata aaacagtccg gcaaagatac agccaatggg attggaggaa ccgatcaggg
    23641 ccactgtgat tccctgaaag ccatagcctt cctggcctgc aaactggctt aaacggcctg
    23701 ccatgccaag gatctgaacc gctcctccaa gacctgccag cgcaccggaa atactaagag
    23761 ccgtaagcac agacgcatca gagttaatac cgccgtacag agcgccgttc cggttaaagc
    23821 ccactgcttt cagtttgtat cccagtgtcg ttttttcgat gatcacccag atgatcacag
    23881 cagccgcaac ggccaggatg atcccccagt ttgcaacctt gcatcccagt acatctctta
    23941 ctgcctcagg gaagaggata cgggccgttt ctgccacatc ctttgtggct tcaccgccgc
    24001 cttccttgtg aatggcagga atatttacca cataattgga gaggtaaaag gcaatccagt
    24061 taaacatgat aaacgacaaa acctcgtgaa tgccccgctt tacctttaaa agtcctacca
    24121 aataggacca gagcgctcct gctgccgctg ccgccaaaag gcacagggga atatggataa
    24181 cagccggaag ttttaaggta attcccagca cacaggctac caggcttccc actacaaact
    24241 gtccttctgc tccgatgttg aagacgccgg tcctaaagga gaatgcaaca cttaatccgg
    24301 taaagatcaa agggcttgca taggtcagta cccatgcaag atattttggc ttgccaaaaa
    24361 cactgtcgat cagcttgccg taggccactg cagggctgat ccctgcgatc ataaggaaga
    24421 cagctccgat gataaacccg gcaacgatcg caatgagcgt ataaagtaga ttggattcaa
    24481 ggatactttt ctttttattc attcttccgt cctcctgcca tcatatagcc gagttctttt
    24541 tcatctgcat tctttccggg aacgcttcct gtgatcctgc cgtcaaagat gacctcaatc
    24601 cggtcagaaa gactcatgat ctcatccagc tcaaaggaaa ccaaaagaac ggccctgttt
    24661 ttatttctct gctctaccag atacttatga acaaattcaa tggctcctac atccaggccg
    24721 cgggtaggat ttacagcgat cagaagatcc ttgtcattgg tgacctctct ggcaataatg
    24781 accttctgct gattgcctcc tgaaagcgtt cctgccggcg cttcttcgca gcctctggga
    24841 cgcacatcaa atttttcaat cagctctgcg gcatgggcgc ggatcgcttt ccgatttaga
    24901 aatcccttat ttgagaaagg ggcctcctca aaatgctgca ggatcaggtt gtcctcattg
    24961 gaaaagtcca ggatcagtcc atgcttctgg cggtctgccg gaatactgga aataccgctt
    25021 tcaaaggttt ctttcggcgt tttattgata acctcttttc ccagcatctt cacgctgccg
    25081 gattctgcct tcttaaggcc tgtgaggatt tccaccagct ccgtctggcc gtttccgtcc
    25141 acgcctgcaa gacctacgat ttctcccctg cggaccgtta aattaagtcc ctttaagatc
    25201 tctgcgtccc gataatccct tgcccttaca tcctttacct ccagaacgat tcctcccggc
    25261 tcctgctcct ttttatccac cttaaaattc acatcacggc cgaccatcat ggctgcaagg
    25321 tcattttctg ttacctcatc cacctttacc gtgcggatat atttcccctg gcggataatg
    25381 gtgcagtgat ccgaagacgc cgtgatctcc ttcagcttat gggtaataag gatcacgctc
    25441 tttccatccc ttgtcagatt ctctataatc tccatcagtc cttcaatctc ctgaggggtc
    25501 agggaagcgg taggctcatc caggatcagc gtatccgcac cgcggtaaag caccttcagt
    25561 atctccacac gctgctgcat cccaacggaa atatcctcga tctttgcgtc aggatcgacc
    25621 ttcatgccat aacgctccga cagctccatt accttctgtc tggcggctgc cttatcgatg
    25681 gaaagcccct ttacaggctc aattcccaaa ataatattat ccgttaccgt aaatggctgg
    25741 atcagcataa aatgctggtg gaccataccg atcccgatgt cgatagcatc ctttggactg
    25801 gaaaaatgca cctctttccc tttaataaaa atctgtccgc tggtaggctt ataaagcccg
    25861 cagagcacat tcatcatcgt acttttaccg gctccgtttt ctcctaaaat ggcatgtacc
    25921 tcacccttat gcaccgtcag attgatgtgg tcattggctg taaaattacc gaatttcttt
    25981 acaatgtcct tcatctggac catgacctgg ctttcatcca tatggctggt ctttcccatg
    26041 tatattccct cctttttcat atgatcacaa tatctttaaa tcccatctcc ctcagtatgt
    26101 ctgccagttg cgtcaaacat tcaggagacg gaacctggta gtgggaatac gcttccctca
    26161 ctcccatggg acggtacgcg atcagcttga tacggatttc gcgtatattc cggtaaggcg
    26221 ccagaagcgt tccgattccc cggatgcttt tttctccgtc atagagatcc ggaacgatca
    26281 cagcccggac ttcctccagt tttccgtgct ttgcaagaaa aaccgcattt ttaagcacga
    26341 tcccgttgcc ttcatcggtg atccggcggt ggtctgtatc atcaaacgcc ttgatatcca
    26401 gcattacgcc gtcagatatc tctaaaagct ccggatagtc ccataaaggg atagtcccat
    26461 tgctgtcaat gagacagcca agtccgtctg ctttggcaag cgtaaacagc gcctttaaaa
    26521 aatccggcca gagcatacac tctccgccgg atactgagat gccccgtata aagggaatct
    26581 gccgttttac agccgcatat acctcctccg gcgtcatttc ccgcactttt ggggaactgt
    26641 cattgggaca gacatggata caggtatcac actgcacgca ggctccctga tcccagatga
    26701 cccggttatc ctctcccagg gacagcgccc cggcagggca ctgtcccaca caggctccgc
    26761 agtttataca gagccttctt gtttcgggat tatgacaata tttgcagtta atgttgcagc
    26821 cctggaggaa aatggcagtt ctgtttccag gaccgtctac actgctgaaa ggaatgattc
    26881 gatttacaat ggctttcatc agcgcacctt tctctccagg atatgaccgt tcttggaagc
    26941 tcccagcccc agaccggttg tgttctggag aacggcctgt ccctgatcca gtttttccat
    27001 ttcagagcgt tttaccagat aaccggtcac tcggatcaca tcgctgtcgc tggaatagaa
    27061 ggacagatag cgcagatcca ttttaaaggc ccccttaatg atatccagca catattccgg
    27121 gttgcggtgc accgtcatat cgattgggaa aatatctccg gttccggagg taaaataccg
    27181 gtggaaatgc tcaagcacct ttaactggtc gatcagctcc tctggctcct ccccgatcgg
    27241 gatacgggtt cctggagtta tatcaatgtc ttccgcaatt cccacctggg catgaagcag
    27301 gaagcggcct cctgttccct cacagtaggg atttacatgg actgcgttaa aagcctggat
    27361 ctgatccata atttcaacgc ccagccgcgt agcgatctca tcatggccaa accgtccctc
    27421 tataccttcc ttttcaagaa ggatattgac gcactctgcc aggcctacaa gaccgtacat
    27481 gccggagaaa cgatccctgt ggataaagcc ctccttcgca aggaagtgat gttcaaagaa
    27541 tccgctttcc tctacctcaa accggattct ctcatccatg taccgtgcca taatatccag
    27601 tacatacggc agctgctgtt ctttaaaatc ccggatatcc tttgcgcgct ttgcaatatt
    27661 tcccaggatc aggcggcaca gcgtataagc gccgccaccg taaagcaggc cgttatagca
    27721 gcttgcgatc acatagcctt cacccaattc acccttaaac atcttatggt ttgcgaagct
    27781 gggctttgca ctgtgcagtg cgcaccggat ggcctccagg gcaaagtcat cttctgtaac
    27841 gccttcctca tatttcatgg taatgttggg aaccgcatgt tcaagctccg cttccacttc
    27901 taacagcagg cggcctgcct tggtcgccct gggaccgata tttgcatgag agaaggagtc
    27961 cagtaccgtg cggtcaatgt gcgtcataaa catccctaaa agctttttcg cctgagcctc
    28021 gtccactgta tcaataaaag gctccagcag ttcatctaac tgtcccacat aaaccggata
    28081 gttggtaacg ctgggaacat gcttataaaa gatcagcaga ttattaatag cctcatacag
    28141 atctgtcggc ggtttaagct ggagaaattc actgccctct ttcataaatt ttgcataatc
    28201 cgggataatg taccggggac gcatgggcgc atggccttct gccagatcac agatgcactt
    28261 acggtctgca ggaacattta aaagttcatc cagcccttcg gggaggtcca aaacctccat
    28321 cagggaatcc gcctggcagg ccaggttcgt aagcttctgc tcatgggtca gcacagggct
    28381 ttttataatg ctcaggatat tctcccttgt ttcatttaca cgctccattg aaatgtcacg
    28441 catatatggt tcctccttct atcattgttc aatcttagtc taactgatat acatcgccgt
    28501 atgcagcctc aaactcttcc ttgctggcag gaacagtcac ttcgcctgca aggatcttag
    28561 ccttaacatc ctctactgcc ttctttacat cctcaggaag aagatcctgg gaaggagcaa
    28621 tatctacgcc gcctgctgcc aggtcatacg tatgaaggcc gccctctaag gtaccgttaa
    28681 gcagagactc tacactgtca taaacagcgg tatctacacg cttcatagcg gaagtaatta
    28741 cggttccagg tgcgtcattg tactggtctc tgtccacgcc gatagcatat acgcctgctt
    28801 cctggcatgc ctcgattacg ccaagtccgg ttgcgcctgc tgcatggaaa acaatatcag
    28861 cgccattggt gatcgcggta cctgcgtttg tttttccggt tgccggatct gcaaaggagt
    28921 ttgcgttaaa ctgctggatc gtaatatccg gatttgcatc aatagcacct gcacaatagc
    28981 cgtatccgaa caggttcatg gtttcatttg tcataccgat cactacgccg atattgtcag
    29041 tctctgtcat aaggccggca acgtatccta ccagatagga agcctgctcc tggcggaaca
    29101 tcagacaagt aacattgggc aggtcaatgg tagcgtcatc aataatagca aacttacact
    29161 caggatttgc ttccgcagcc tgcttggtag cgtctgccag catatagcct acgctgatga
    29221 tcaggtcgta ctcttcatca ataaacgtct caatgtttgg aacgtagtct gcatcagtag
    29281 aggactcaag ataatttacc tcaacgccaa aatcctctgc cgccttctga agaccctccc
    29341 aggaagactg gttgaaggaa ccgtcattta caccgcctac gtctgttaca agaccgatct
    29401 tgtattcgga tccgtctttt ttctcacctg ctgcctctgt ttcagcttct tctttttttg
    29461 ctgaagtatc agcggctgcg gaagactctg cctttgtctc agaagtgccg cctgaacctc
    29521 cacaggcagt cagagatact gccatggctg ctgccaacat aagtgctgct gttttctttt
    29581 tcataatgat tctctccttt taaagtatat aatgaagccc tgcttttaag caaagcccat
    29641 gtgaagctgg aaaaatcccc tgcttatgaa acagggattt ttccagcctg ctgcctttta
    29701 taaaactgcc tatttctgtt cctggcggat cagttcccgg atggccccta tggcggtgcg
    29761 gattgccata tccgtatcat ggaccaccgg attttccagg cccagctttt cccgctcctg
    29821 atttgcaaca accaggaata cggaaccggc ccgtacatga agcgctccgg ccacgataaa
    29881 taatgcggct gattccattt cggatgccag acagcccatc cgtttccagg cttcccattt
    29941 gttcaaaagc tcatagctta caggcatgcg ctccggggag tgctggccgt aaaaagcatc
    30001 cttgcactga actacgcctg tatggaaagc aaggcccagt tcctttgcag aagctgtcag
    30061 cgcatttgtc actgtaaggt cagcaactgc cggatattca atcggagcat actctctgct
    30121 ggttccttcc atacggatgg ccccggtggc aattacggca tcaccgcttt ttacctccgg
    30181 ctgcatccct ccgcaggtgc ctacacggat aaaggtatct gctccgcagt ttaccagctc
    30241 ctccatggca atggaagcag aggggccgcc gatacccgta gaagtaacgc ttacctttac
    30301 tccatctaac gtaccagtat aggtcacata ctcccggttg tcagccacta ataccggatc
    30361 atcaaaatac ttcgcgatct tttcacagcg ttttggatct cccggcagta tcacataccg
    30421 tccgacctct ccttttccca cctgaatgtg atattgtttg ttaggatctt ctgaatactt
    30481 cacgttctgt tcctccctgt acgtttttta attattataa accagccgcg gctgattcct
    30541 aactgtcaat ttcactcatc tttgcatccg ccagagcttt taagcgctgg tactggctgc
    30601 tgtccatagt gatattccat cctttttttg taatatatcc ttctgatacc agactcttga
    30661 tcgctctggt gatcgtccgt tctgaaaggc ctgtcatctc agaaaaatcc tttcggctga
    30721 tataggcgct gtatgttcca ttttcctcat aggatttata taatttgatc aatacctgat
    30781 aaacccgctg atttccggac aacagcacat acagccgttc ttttcttgcc tgctccagaa
    30841 gataattgac aatttccttt gtttccatct gaaatgcatt gctgtcgctt ttcagccatc
    30901 gctcatactg gtctcttcca atccgcagga aaatactttt ttccactgct gcaagagagg
    30961 tcttataccg ctccattccg cctaaaattt ccatcacccc aaaaagttca ataggataaa
    31021 actgaaagaa accatatacg gcctcctgta cccggtagtc aaccgcagac acctgtccct
    31081 gtaacagcac ataaacgcgt tctgccggct ctccctcatg gataaaaaca gtccctgccg
    31141 gaacctcgat cacctgaaac tgttccagca gccaacgggg agcgcctgca aaatactgtt
    31201 tcagataatc caccttttcc ggattcagct tttcaatctg cttctgagtt tctatattca
    31261 gtttcttaat ctgcttttgt gtttccttca taaaggattc ccttttgtat atttcttcca
    31321 tttaacttgt tttcatcata tcacattttc acaaagaaca aaaggacata tgtcactttt
    31381 caaaaaggaa aaagagtttt tttgatgttt ggcacaaaaa accaccatat ttttttgcgt
    31441 atatggtggt ttttacctat agctatataa aattactgtt tctgcttact tttcagattc
    31501 ttcacttctt ttatatacct cttccagagg aatctttgcg ccattctgcg ttatggcgct
    31561 tacaaggaag attgctataa aagccagggg cacggagatg atcgcactct ggacgccgcc
    31621 cggagaacgt aagaccacat cccagatgat cgtcgtcacc gctccggtga tgatgcctgc
    31681 cagaccgcct gccttggttg cttttttata aaacagcgca cagatcaggg ccggtgtaat
    31741 ggttgctcca tacatggtat aggcatacat ctgaacggaa aggatatccg ggaaataaaa
    31801 gcccagaaca aacgccagaa aagccacaag gacaatggaa atacgaagca tattcagttt
    31861 ctgcttatca tccgcatcct ttttgatcag gcgcacccat acgtcataag tcaggttgga
    31921 agccgtcgac agaagatagg agtcacccgt agtaataaca aaactcatgc aggcagccat
    31981 tacgataccg cctaaaaccg taggaagctt ctgtgctgcc agctggaata aaatggtatc
    32041 tgcagaatcg ttggttggat acagaggaat ggcacaggtt acaagaccga taatcagggc
    32101 acatacaaca acctctgcaa ttacaaggcc cacattggat ttgcgggcgg tgcggctgtc
    32161 ttttgcggaa ctgaaccgct ggatcatgtt ctgatcaccc agcaccaaaa acagcgttgg
    32221 cagcacatag cccagcacct gagcaatatt catagttcca aagatggaat ttttctctgc
    32281 gggaacctga gccagtacgc cgccaaagcc accgaaatct ccgctcagat ataaaatacc
    32341 gaacatgaag ccaaacacca tcataaaagc agataaagca tccgtatatg ccacgctgac
    32401 cataccgccg atcagagcca gaagcgccat aaatatcacg cagatcacag tggaggtgat
    32461 aaaatcaatg ccactggtaa tcataaaaat attgccggct gccttaaact gggaagctgt
    32521 ggttccgata tacgccagaa taatgcagat tgcagccaga tttctggcag ctgctccgta
    32581 gcgcagctca aacagctccg gaatagtata tgtggtactt ttgcggactg ctcctgaaat
    32641 aaaataaaga acgatcattc cgatcggagc tcctgcaaac agcaggatac caaacagagg
    32701 tccgttttta taaaccaggc ctgcggaacc cgaaataccg ccgccgccgc accatgtagc
    32761 aagcagcgtt cccactaata cgatcatggg aaggcgtctt cccgctaccg caaagtcatc
    32821 tgagcttttt accatttttc tttttaaata aagactcact cccaccatca ggagcagata
    32881 gcccactacg aataaaaacc gtacatccat atcattttcc tctcttttat catacctgtt
    32941 ccatctgcat ttttatccga acattattcc ttatgggctg cataaagctt cagcagccgg
    33001 atcaggatct ccacattttt cgccatagcc tggatggaaa caaactcaaa tctgccatga
    33061 gcattttcat aacctgctgt aatgttgggg catggaagac ccatctggga aatccaggaa
    33121 ccgtctgtgc cgcctctcat tgcgatctca aagggctcca ctccgcattc tttcatagcc
    33181 tgcttgcagt agtcgatcat aaacggaacc tttttgatga cttcccgcat gctgtaataa
    33241 atgctgtccc atttaagctc cacggttccc tcaccatagc gccggttcag ctcctctatg
    33301 caggatataa cataggcttt tctctcctcg taccgatcca gctcatgatc ccgaaccaga
    33361 cagcggatat aggtcttttc aacagttcct tcaaaaacaa agggatgaaa atacccttcc
    33421 cttccttctg taaactgagg ccgttccaga gccgggagca tagccataaa ttctccaccg
    33481 atctccactg catttttcat aatgcccttg gctgttcccg gatgaacact caggccatgg
    33541 atagtcaact gagcttccgc accgttgaag gtttcataca ccagaccgcc cagggcgtct
    33601 ccgtccatgg tatatgcaag atctgcccca aaacgctcta cgctgaaatt ttccgtgctg
    33661 cgcgcgatct cctcatccgg agtaaagcac a
//
LOCUS       DBMDUH010000008        30404 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_1127_length_30404_cov_6.348183, whole genome shotgun sequence.
ACCESSION   DBMDUH010000008 DBMDUH010000000
VERSION     DBMDUH010000008.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 30404)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 30404)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30404
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1127_length_30404_cov_6.348183"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="ACZPCG_01680"
     CDS             complement(<1..186)
                     /locus_tag="ACZPCG_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1283814.1"
                     /translation="MSMSDMEFRAVLLDQMKDYLDGRITKEEYYDQAEPFYTEYANTY
                     ENPLFHKCFMDTVPEACL"
     gene            complement(311..571)
                     /locus_tag="ACZPCG_01685"
     CDS             complement(311..571)
                     /locus_tag="ACZPCG_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1283815.1"
                     /translation="MATLEEKLRWAEEDFRAQVREERLAYASLTWLIGKVCKKALKGK
                     DAVTIANELEEDDVGKVQRIMDVAKSFAPDYDVEEIYKKLME"
     gene            742..996
                     /locus_tag="ACZPCG_01690"
     CDS             742..996
                     /locus_tag="ACZPCG_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1283816.1"
                     /translation="MARRRSGLFYIRFQEVISHVQEHKRKQAFSGFLAFSIKKEISDR
                     LKILFPFFVPAAAQYVFAAEKKKPQPVRVYRLSVVFSNLL"
     gene            complement(1059..2333)
                     /gene="purD"
                     /locus_tag="ACZPCG_01695"
     CDS             complement(1059..2333)
                     /gene="purD"
                     /locus_tag="ACZPCG_01695"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HIR1283817.1"
                     /translation="MKILIIGGGGREHAIAWKLSKSPRVEKIYCAPGNAGIAEIAECV
                     NIGVMEFDRQVAFAKEHGIDLTVVAPDDPLAAGAVDAFEAAGLRAFGPRANAAILEGS
                     KAFSKDLMKKYNIPTAAYETFTDPEEAIKYLETARMPIVLKADGLALGKGVLICSNLE
                     EAKEGVRTLMLDKQFGSAGDEIVIEEFMTGREVSVLSFVDGKTIKIMTSAQDHKRAKD
                     GDQGLNTGGMGTFSPSPFYTSEVDAFCREKIYQPTVDAMRAEGREFKGIIFFGLMLTE
                     DGPKVLEYNARFGDPETQVVLPRMKNDLADLFEACIDGTLDQVELEFEDNAAVCVVLA
                     SDGYPEHYEKGYPISGLERFKDADGYFVFHAGTKLNDQGQIVTNGGRVLGVTATGGDL
                     KEARANAYKATEWVEFGNKYMRNDIGHAIDEV"
     gene            complement(2350..2943)
                     /gene="purN"
                     /locus_tag="ACZPCG_01700"
     CDS             complement(2350..2943)
                     /gene="purN"
                     /locus_tag="ACZPCG_01700"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008395144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HIR1283818.1"
                     /translation="MLRTGIMVSGGGTNLQAILDAVDSGKITDTEISVVISNNPGAYA
                     LERARTHGIEAVCISPKEFESREAFNKAFLEKVDKYKLDLIVLAGFLVKIPEAMVKKY
                     EGRIINIHPSLIPSFCGVGYYGLKVHEEALRRGVKVTGATVHYVDGGMDTGPIILQKA
                     VEVEKGDTPEILQRRVMEQAEWIILPKAIQMIAHGEV"
     gene            complement(2937..3968)
                     /gene="purM"
                     /locus_tag="ACZPCG_01705"
     CDS             complement(2937..3968)
                     /gene="purM"
                     /locus_tag="ACZPCG_01705"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HIR1283819.1"
                     /translation="MDYKKAGVDIEAGYKSVELMKEHVKKTMRPEVLGGLGGFSGAFS
                     LSKIKDMEDPVLLSGTDGCGTKVKLAIIMDKHNTIGIDAVAMCVNDIACAGGEPLFFL
                     DYIACGKNYPEKIADIVKGVAEGCLQSEAALIGGETAEHPGLMPEDDYDLAGFAVGVC
                     DRKEMITGESLKAGDVLIGMASTGVHSNGFSLVRKVFEQEMTAEGLNTYYDCLGKTLG
                     EALLAPTRIYVKALKSIKKAGVAVKACSHITGGGFYENVPRMLKDGVRAVIKKDSYEV
                     PAIFKLLQEKGNIAEEMMYNTFNMGIGMIVAVDPADAEKAMEAIRAAGDTPYVIGSIE
                     EGEKGVALC"
     gene            complement(3988..4500)
                     /gene="purE"
                     /locus_tag="ACZPCG_01710"
     CDS             complement(3988..4500)
                     /gene="purE"
                     /locus_tag="ACZPCG_01710"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005348104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HIR1283820.1"
                     /translation="MAKVGIVMGSDSDMPVMAKAAEILDQLGIDYEMTIISAHREPDI
                     FFQWAKSAEEKGFKVIIAGAGKAAHLPGMCAAVFPLPVIGIPMKTSDLGGVDSLYSIV
                     QMPSGIPVATVAINGGANAGILAAKILAASDSGLLEKLKLYSEGLKNDVVKKAEKLEK
                     LGYKEYLAQM"
     gene            complement(4646..6736)
                     /locus_tag="ACZPCG_01715"
     CDS             complement(4646..6736)
                     /locus_tag="ACZPCG_01715"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002575295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HIR1283821.1"
                     /translation="MDYRILLREENEAVRERLELSLDRIRSIKEEDARLPYGLYFKRT
                     AGFLGMIRELLTARGIGEKELPESWEKLSLEELSDWNHRLYEDILPENYGESFANPDY
                     AADCLGKEFGPLLSFLYTELRGCIVYAFEMRLEYIAILCETFLEVYNLFTQAWEEENE
                     TPREQAVRDALYWYVSDYVDLHLPWRIREGLDPALSFAADIIMDSDLTDLRYLYQYGE
                     YVSDSERRLAAFMASLPEETIEKMAHTYTEGYRRGFEVMGRDLSKKKTVVVEYQLGFE
                     RMIRRAVEQFRSLGLETVCYRAAVESLNRRSNGKRGYYGTSANRQYDYDHRYDSALYM
                     GNAFKERKLSILKTAYEEYKDLAAMCAGPAVVETFGEEEFEPVNKKTAFSLNDHQQEL
                     TLSYATASRQIVNQYMPGDETSFTIIAFPRPEIGPDFEEIFKETIRINTLDYDTYQKI
                     QQTLIDALDQAEYVRITGREGNETDLTVHLHTLNDPSRETNFENCVSDVNIPLGEVFT
                     SPVLEGTKGLLHVKEVYVREYLFKDLRLEFEDGRVTEFSCGNFDSGKELVKQVILNGH
                     SWLPLGEFAIGTNTAAYAMAKKYNIGSKLPILIAEKTGPHFAVGDTCYSFAEDSPMYN
                     PDGKEVIARDNEISLLRKEDMSRAYFSCHTDITIPYNELGDITAVKADGSEILLIQAG
                     QFVLPGTEELNRAL"
     gene            complement(6751..7086)
                     /locus_tag="ACZPCG_01720"
     CDS             complement(6751..7086)
                     /locus_tag="ACZPCG_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007037100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6142 family protein"
                     /protein_id="HIR1283822.1"
                     /translation="MEALRQVGNKISYVRNPFARQSFFCLGLGILALGLGTFSIYLSL
                     KGAGQGGLNTGAYGFSSIVAAAAGLWYGILSFTEDNCNYILAKIGIALEGILIIIWAV
                     ILIIGFTGR"
     gene            complement(7074..7547)
                     /locus_tag="ACZPCG_01725"
     CDS             complement(7074..7547)
                     /locus_tag="ACZPCG_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HIR1283823.1"
                     /translation="MIRHTTPRQKAGWVLFILYLCLLAYFMFFSESFGRTETDREYAY
                     NLVLFKEIGRYFRYYEVLGFGLFMINIVGNIAAFIPFGFFLPIISKRSRLWYNTVMFG
                     FIWSLMLETLQLILRVGSFDVDDMFLNTLGAGIGFYIHGIAHYVRVLIHYRRWKR"
     gene            complement(7561..7710)
                     /locus_tag="ACZPCG_01730"
     CDS             complement(7561..7710)
                     /locus_tag="ACZPCG_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002600276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1283824.1"
                     /translation="MERVYKTMRNTGAGCIAVGIIIAVTGLAVGIISIVNGALLLKRK
                     SEIEF"
     gene            complement(7764..8441)
                     /gene="pyrE"
                     /locus_tag="ACZPCG_01735"
     CDS             complement(7764..8441)
                     /gene="pyrE"
                     /locus_tag="ACZPCG_01735"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HIR1283825.1"
                     /translation="MEQYKQEFIEFMVESNVLKFGDFTLKSGRKSPFFMNAGAYVTGT
                     QLRKLGEYYAKAIHDNFGLDFDVLFGPAYKGIPLSVATTMAISELYGKDIRYCSNRKE
                     VKDHGDTGILLGSPIKDGDRVMIIEDVTTSGKSIEETFPILKAQGDVEIKGLIVSLNR
                     MERGKGTKSALDEIKDLYGFPTAAIVTMADVTEYLYNKECGGKIVINDTLKAAIDAYY
                     AEYGAQA"
     gene            complement(8596..9408)
                     /locus_tag="ACZPCG_01740"
     CDS             complement(8596..9408)
                     /locus_tag="ACZPCG_01740"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012198811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIR1283826.1"
                     /translation="MEYKMIVLDLDGTLTNRDKIITPRTKQALMEAQKMGKIVVLASG
                     RPTAGIKPLAEELELAAYGSYILSYNGGMITNCGTGEVVFSSLLPVDSNEKIIGLAEE
                     YGVDILTYEGEEIITNNEECPYAVKESNINHLPLRQVEDMKGYVSFPVPKFLMLDDGD
                     YLVTVEPRVKAAMGRDFSVYRSEPFFLEVMPKGIDKAQSLGRLLERIGMKREEMIACG
                     DGYNDLTMIQYAGLGVAMENAVLPVRNAADYITASNNEDGVGLVVEKFMLGG"
     gene            9824..10846
                     /locus_tag="ACZPCG_01745"
     CDS             9824..10846
                     /locus_tag="ACZPCG_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1283827.1"
                     /translation="MYATHTRKLWPLLTAGILAALSLSGCQSSLSPDGQTAVTLSEVA
                     HSIFYAPQYAAIELGYFEEEGIDLTLVNAGGADKVMTSLISGDAQIGFMGSEASIYVY
                     QEGSDDPAVNFAQLTQRAGNFLVGRQAEEDFNWQDLRGKTVLGGRAGGMPQMVFEYIL
                     KKNGLDPVNDLTIDQSISFGLTAAAFTSSAADYTIEFEPFATTLEMEGSGAVVASLGT
                     ESGYVPYTAYSARKSYLKQHPEVIQSFTNAIQKGLDYVNSHSSEEIAKVISPHFKETP
                     VDKLALIVDRYKSQDTWKEDTVFEENSFTLLKRILEEAGELKAEVPYNELVTMDFSKK
                     AAKNSQ"
     gene            10975..11919
                     /locus_tag="ACZPCG_01750"
     CDS             10975..11919
                     /locus_tag="ACZPCG_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003500817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding periplasmic protein"
                     /protein_id="HIR1283828.1"
                     /translation="MKKTVQMLIVLASAAALTAISGCSAIGSAPTLPSETIPDTISAS
                     SEAAKAASEETTGSNGGSRLNDILTRGYIEVAMEPYFAPNEFIDPSRQGEEQYVGADV
                     ELARYIADKLGVECRIVPLEFSAVLTGVTEGKYDLAISALAYTPARAEAMEMSKGYRF
                     DEDNANNFGLMIRSGDLDTIKSADDLTDKIVVCQSGSLQELFATEQIPACRELKRVSA
                     TTDGFLMVQEGKADAVVTEKKTAELFIAANAESSMVIVPDFSFVVDESTSGTRIGIPK
                     GETALLNKINEIIDEVTASGIYDEWYEKYTEYAKTLGL"
     gene            11980..12645
                     /locus_tag="ACZPCG_01755"
     CDS             11980..12645
                     /locus_tag="ACZPCG_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003500815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR1283829.1"
                     /translation="MWATIIKMWNRYSYVYIQGLTGTLCLAAITVLCGTVLGTLLAIM
                     RRSRIKPFQWFVQFYVWVLRGTPILLQLYFFWIFLPRATGIKIPDSTCIIIALIVNAS
                     SYVSEIIRAGIQAVDIGQTEAARSLGMTARNTMTKIILPQAVKNILPALGNEFITMIK
                     QASLASVFFINELTTSYKTVASATFRQVESIIIAGIIYLLVTTILGRIMAYAEWRMKQ
                     NER"
     gene            12635..13396
                     /locus_tag="ACZPCG_01760"
     CDS             12635..13396
                     /locus_tag="ACZPCG_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIR1283830.1"
                     /translation="MNAENKICAEARPIIQVNNLTKSFGKLEVLKGISETIYEGEVVS
                     IIGPSGGGKSTFLRCLNLLETPDSGTILFRNSNITDRSVNVDQYRQHLGMVFQHFNIF
                     PHLKVIDNVTLAPVLEKKIPKAQAKAAALRLLERVGLSDKKDEYPNRLSGGQKQRLAI
                     VRALAMEPEIMLFDEPTSALDPEMVKEVLNVIRDLASSGMTILIVTHEMGFAREVSDR
                     IFFIADGIVQEKGSPEELFLDPQNPRTREFLGKIL"
     gene            13416..14858
                     /locus_tag="ACZPCG_01765"
     CDS             13416..14858
                     /locus_tag="ACZPCG_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009297234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR1283831.1"
                     /translation="MKTLEFIEKIIEEKSTRIIEANDQIWEFAELAYHEYQSAGLLCQ
                     FLKDEGFTVETGLAGIPTCFQGTYSYGCGKPVVGILGEYDALSGLNQAAACPSRQPII
                     QGAPGHGCGHSALGAGALAAAIAVKEYLKTFKKGGTVIYFGCPAEEGAGSKQFMARAG
                     LFDQVDFVYTWHPDSINQVDNSHNNAIMGANFHFKGLTSHAGSAPYLGRSALDAAELM
                     SVGCNYLREHMIPEARIHYAYIDAGGTAPNVVQDRSTVRYEVRSPYVRQLTELFNRVV
                     DVAKGAALMTGTSMNYDLSMAFTEYLPNKALAQIADACMQEVGAPKWTEEDYRLARQF
                     LDSYDDTARKMIRDEIIQIYGEDRLEEILSKPLDSEIHPFDPNHIIQTAGSTDVGDVG
                     YAVPTLNLRVAACCVGNVGHTWQMTAQTCSPIAHKGLLTAGKMIALSAIRTMERPDII
                     DAAKTETLKRNSGHYTCPLPDHVLPPLETY"
     gene            complement(14855..16141)
                     /locus_tag="ACZPCG_01770"
     CDS             complement(14855..16141)
                     /locus_tag="ACZPCG_01770"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007038631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR1283832.1"
                     /translation="MKRTKAAGFLLALLMTAGQVTAALASEPELALPAGVPARAVSRE
                     TAQIQAEAGDAAASQTAIQISAPSAVLMEASTGQIIYEKGADDKRSPASVTKVMTLIL
                     IFDALAEGKIHLSDEVVTSAHAKSMGGSQVFLEEGETQTVETLIKCIVIASGNDASVA
                     MAEYISGTEEAFVQQMNERAEGLGMTKTHFVDCCGLTEDPSHVTTARDIALMSRELID
                     QYPQIHNYSTIWMENITHVTKQGTKEFGLSNTNKLLRMATNFKVTGLKTGSTSLAKYC
                     LSATAEKDGVRLIATIMAAPDFKARFADAQTLLNYGYANCKLYEDKEGITLPEISVEG
                     GIKSGAELVLNGTFRYLSLKGEDLSSVEKKLVLSESLPAPVEPGQKAGVLEYLLNGEK
                     IGEMDVVTAEAVREAQYGDYLKWMIKIWRLEKAFDF"
     gene            complement(16277..16717)
                     /locus_tag="ACZPCG_01775"
     CDS             complement(16277..16717)
                     /locus_tag="ACZPCG_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HIR1283833.1"
                     /translation="MAEMVKDPNCAYCMQGELVAKFAYPVCEMKTGFLYVFKEQSKKG
                     RVILAHKKHVSELIDLTDKERNDFFAEVAQVARAVHKVFHPDKVNYGAYGDTGCHLHF
                     HIVPKYKGGEEWGGTFEMNSGRTILTDAEYEEMAEALRQALKEV"
     gene            16900..18411
                     /locus_tag="ACZPCG_01780"
     CDS             16900..18411
                     /locus_tag="ACZPCG_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HIR1283834.1"
                     /translation="MSRNDFRRRQAQRQRRKLVRHLPIILALFLAFSALAAGVFALTR
                     FVLNRQPISSASASSSTAKTTSEDPAQPISSPASGTLAQAAYIAAGYDYDRAIALIKE
                     DSSLAEDPAALEAITSYEEAKASLLPADVQNVPHVFFHSLIMDTAKAFDGDSRTPGYN
                     SVMTTKDEFLKMLDAFYEDGYVLVRIRDIASETTDENGNTVFSWGEILLPEGKKPLVM
                     SQDDVCYYPYMEKDGFARRIVIGEDGKPTAEMVMEDGSISTGPYDLIPILEDFIEEHP
                     DFSYKGARAIIAFTGYEGILGYRTAASYSDSPNYEQDREQAAKVAQCLKDNGWELASH
                     SWGHLWMGVSSVPGETFKISDERFYTDTDKWEAEVESLIGPTDIYIYPNGNDVADWRP
                     YTEDNYRFKYLHSKGFRYFCNVDASKPAWIQKGSDHLRMARRNLDGYRLYEDMIQTDP
                     SKKRLSDLFDASQIFDASRPTPVTWSYTQNEAAADASAPAAPETVPSESAASQ"
     gene            18489..19256
                     /locus_tag="ACZPCG_01785"
     CDS             18489..19256
                     /locus_tag="ACZPCG_01785"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1283835.1"
                     /translation="MTPKLDVNGVSFSYHTLEGETLALSDISFSLSPGEFAAVVGPSG
                     CGKSTLLSLLCGLSKPDTGQILLDGHPLRQSPSDTGYMLQKDHLFPWRDILSNVSLGL
                     EIQNRLTPKTTARLKEMLSAYGLEGFEHARPSELSGGMRQRAALIRTLALEPDLLLLD
                     EPFSALDYQTRLSVCDDISSILRSTKKTAVLVTHDLSEAISIADRIIILTPRPGRIKK
                     ILPIHFPADCATPLKRRNCPEFSGYFNAVWKELKTDE"
     gene            19249..20052
                     /locus_tag="ACZPCG_01790"
     CDS             19249..20052
                     /locus_tag="ACZPCG_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003021034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1283836.1"
                     /translation="MNNPLLSPSLAQQEYLADQLRRRSMIRIWRILLLILILSLWELS
                     AGLGLIDRFIFSSPSLIFKCLISMTADRTIFLHTGVTLMETLISFAVCTAAGLVCALL
                     LWASPSAASVLEPFLVLLNSLPKSALAPLLIVWLGNNTTTIVTAAVSVALFGSILTLY
                     NGFCQTDEDKIRLIRSLGGTRTDVLFKVLLPGSLPMVINNMKVNIGLCLVGVIIGEFL
                     SARAGLGYLITYSAQTFTMTVLMTSIMILCLLSWLLYQAIACVEKKLTR"
     gene            20176..21432
                     /locus_tag="ACZPCG_01795"
     CDS             20176..21432
                     /locus_tag="ACZPCG_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT domain-containing protein"
                     /protein_id="HIR1283837.1"
                     /translation="MDLINQFIDNYKKKFNYYESVGRLAAGQLEAVLRSSGIRAMVTY
                     RAKNPGRLKSKVLRRNVKRPVPYRNIKEIYEDIADLCGVRVSLYFPGDRLQADQLIRD
                     QFHILESKQFPEQSKPPTYHKRFSGYWANHYRVYLREELVQPSEKRYCRARIEIQVAS
                     VLMHAWSEVEHDLIYKPLQGTLSKEELAILDELNGLVLAGEIALERLQAAGNERIRSK
                     NAVFNNQYELASYLYNYLSSNFRPEDIELRMGNIELLFRLLSSLKLLDVKYMGPILKA
                     VKFEKDKRNISQQIIDQIISGNEKRYLAYQSLKAAGSKEDEERLKAISAFFSQWVPLE
                     ALLNRTTKRSSRSLSVFNINALKRTGLFDKDSLNQIAFLRKMRNTLIHDIEVPEISCI
                     RELAEQARALNETLLKLLEPEKDEAD"
     gene            complement(21439..22926)
                     /locus_tag="ACZPCG_01800"
     CDS             complement(21439..22926)
                     /locus_tag="ACZPCG_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002573926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR1283838.1"
                     /translation="MPVFDFNHTPETEDTKGAECTYSIFYSDAPQAAPEQPILVLDTK
                     GQQWEHHTCGLFTNPVRGTAFEFKDGGETVSGNILKIDARFVSLIRWLGENHINVRLS
                     GENREEGYSVYRIRETAFGGGTKLSAEDGFLQFMIERLFASSAPSETEEEEETEEGDD
                     MKLTSLQSITDFMTCAGKTLPDHIRLWARRNLAVAKSHEVSPEERRHAQRALSIMMNV
                     RWKHDYFEAIDPVEARRILDEELYGMERVKQRIIETIIQINRTHTLPAYGLLLVGPAG
                     TGKSQIAYAVARILKLPWTTLDMSSINDPEQLTGSSRVYANAKPGIIMEAFSMAGESN
                     LVFIINELDKAASGKGNGNPADVLLTLLDNLGFTDNYIECMVPTSGVYPIATANNKEQ
                     ISAPLMSRFAVIDIPDYTLEEKKIIFSKFALPKILRRMSLRKEECILTEEALDTVVEL
                     YSDTSGIRDLEQAAEHIAANALYQIEVDHLEKVVFDGKMIRELLG"
     gene            complement(23050..23226)
                     /locus_tag="ACZPCG_01805"
     CDS             complement(23050..23226)
                     /locus_tag="ACZPCG_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1283839.1"
                     /translation="MKEIILLGALAVLYKIGGRLFDRLDHFFEGLMPPRESSYEPEER
                     NLEDGWKIEKMKHA"
     gene            23419..24807
                     /locus_tag="ACZPCG_01810"
     CDS             23419..24807
                     /locus_tag="ACZPCG_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HIR1283840.1"
                     /translation="MIEFITSVNTAVNSFIWGVPAMICIIGVGLFLSLRTRFIQIRRF
                     PYAIRTTLGRMFSKKDASEGAITPFQAVCTALAATVGTGNIAGVAGAIAIGGPGAVFW
                     MWISAFLGMCTKFSEVTLAVHFRETNPDGDLAGGPMYYIKNGLGKKWMWLAYLFSIFG
                     ILTVFGTGNATQVNTITTAVRSALTGYGILPDSAVGTFNLAMGILLASSVALVLLGGI
                     KRIGSVAEKLVPFMALLYILLSFGVVFFHLENVPGVFSSIIQGAFSPSAVTGGVVGSF
                     FTSMKRGVSRGIFSNEAGLGTGSIAHACADTDNPVHQGMFGIFEVFADTIVICTLTAL
                     VILCSGIPITYGQDAGAELTILGFTSTYGNWVSIFTAIAMCCFAFSTILGWGLYGARC
                     VEFLLSAAFIKPFMVIYALVSIPGATMDLGLLWSIADTFNGLMSIPNLIAVFLLSGTV
                     IRLTREYFSKNA"
     gene            complement(24903..26168)
                     /locus_tag="ACZPCG_01815"
     CDS             complement(24903..26168)
                     /locus_tag="ACZPCG_01815"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR1283841.1"
                     /translation="MGVLTGALVMAFVGLIIVGMSAGIYLFGRRVISRQPQPGAPSIT
                     EEPAKRVDFEQVTSKMEMIQQIIDQVFLYDEDAQNVEDFIYRGMMAGLDDPYSVYYSE
                     EDYKLMQESTSGTYSGIGAMISQNRTTGLCTVVKVFDGSPALEAGMRPGDVVYKVEDT
                     LVAGESLDVIVSNYVKGEEGTPVQITVYRVDEDAYVDLTLTRKKIEVPTVESRMLDET
                     TGYVAVSEFDLITVDQFKTAIDDLTAEGMKQLVIDLRNNPGGILDSAVAMVDYLIPDD
                     MTDFKKGEGKSLIVYTADRNGEGETYTASDGHAVDLPIAILVNGDSASASEVFTGAMK
                     DYGRAVVVGITSYGKGIVQNLIPLGDGSAIKLTTAHYYTPSGFDLHEKGIVPDVEVPL
                     DESLRTQAVIRPEDDNQLKKAVEVLNNGQ"
     gene            complement(26297..27547)
                     /locus_tag="ACZPCG_01820"
     CDS             complement(26297..27547)
                     /locus_tag="ACZPCG_01820"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003506734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC family protein"
                     /protein_id="HIR1283842.1"
                     /translation="MRKKKIAVLAAAAALTLGTVFSSYATSKAIEDAKKKVSSMEEEK
                     KKVESTLKELEGKKSDTAAYVKELDRNLSALAGELTKLEGDISQKEEQIEEAKVELET
                     AKITENRQYEDMKLRIQYMYENGQTGLLESMMQSESIAELLNRAEYASQITSYDRKML
                     EEYRKTRQEVALKEEALKTEHQELLTLQDSTKAKQSSVKTLMASKEAELASYETKIAS
                     AQGEIDQYNADIKAQEEHMARVEAEIRRKEEEARKAEEARRAEEARRAEEAKKNQSSA
                     GGDSTVKKGNTNFIWPCPASGRISSAFGDRSSPTEGASTNHKGIDIPAPSGSSIVAAA
                     DGKVVISTYSYSAGNYIMIDHGGGLTTVYMHCSQLLVKEGETVKQGQTIAKVGSTGYS
                     TGPHLHFGVRSGGSYVNPSGYVSP"
     gene            complement(27567..28469)
                     /locus_tag="ACZPCG_01825"
     CDS             complement(27567..28469)
                     /locus_tag="ACZPCG_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003526538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HIR1283843.1"
                     /translation="MSTFWYCLKQGIANICRNILFSLASIATISACIFLFCLFFSLAA
                     NVIHGARTAQETVGISVFFDEGMTEDEILAAGEEIKGWKEVRQAEYTSAEEAWDTFKA
                     QYFEGAEDLAEGFENDNPLANSASFEIFLNDISDQESVADRLEAMDGVRRVRYSSTLA
                     AGFTSVGKMIGLISALIIGVLLAVAVFLISNTISVAAAFRRRENEIMRYIGATNFMIR
                     APFLVEGLLLGFLGALVPLGGIWVLYEKGMLYLEENYSMITGLVEPLALGTIFPYMTG
                     GALMLGVGIGLLVSFVTIQRHLRV"
     gene            complement(28527..29198)
                     /gene="ftsE"
                     /locus_tag="ACZPCG_01830"
     CDS             complement(28527..29198)
                     /gene="ftsE"
                     /locus_tag="ACZPCG_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HIR1283844.1"
                     /translation="MIELSHVNKVYSEGNRAVKDLSLTIDDGEFAFIVGRSGSGKSTL
                     LKLLLKELEPTSGRIVVNDMDLGKMPRRYVPKYRRKLGVVFQDFRLLKDLTVFENVAF
                     AQRVIGVPGRVIRDTVPEMLRLVGLSAKYKAYPAQLSGGEQQRVAIARALINRPEVLL
                     ADEPTGNLDSFNAHEIMHLLEEINQRGTTMIVVTHSHEMVQQMKKRVITMERGAVIQD
                     EGGSR"
     gene            complement(29214..30302)
                     /locus_tag="ACZPCG_01835"
     CDS             complement(29214..30302)
                     /locus_tag="ACZPCG_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HIR1283845.1"
                     /translation="MISNQILQTTLDGLKAITRIDLGICDTEGKVLVSTFTDAEESES
                     SILVFVDSPADSQVIQGYQFFKVFDEHQLEYILLAKGASDDVYMVGKLAAFQIQNLLV
                     AYKERFDKDNFIKNLLLDNLLLVDIYNRAKKLHIETDVKRVVFIIETHNEKDVNALET
                     VRSLFASKTKDFITAVDEKNIILVKEVRQGESYGELDKTANTILDMMNTEAMTKVRVS
                     YGTVINDIKEVSRSYKEAKMALDVGRIFYANKNVIAYSNLGIGRLIYQLPIPLCKMFI
                     REVFDGKSPDEFDEETLATINKFFENSLNVSETSRQLYIHRNTLVYRLDKLQKSTGLD
                     LRVFEDAITFKIALMVAKYMKYMESLDY"
BASE COUNT         7494 a         8330 c         6390 g         8190 t
ORIGIN      
        1 caaacaggct tctggtactg tatccataaa gcacttgtga aacagcggat tttcataagt
       61 atttgcgtat tccgtataaa agggttctgc ttgatcgtaa tattcttctt tcgtgattct
      121 tccgtccaga tagtccttca tttgatctaa caaaaccgct ctaaactcca tgtcgctcat
      181 gctcatttat ccactcccat tttatatgtt aaaaattacg gattcctgac aggattatat
      241 cacaactata atcccatgtt caactgatcc gacaaatcga acaccttaaa atcccatact
      301 actattccca ttactccatc agctttttat agatttcttc cacatcatag tctggagcaa
      361 acgattttgc cacatccatg attctctgca cctttcccac atcatcttcc tccagctcat
      421 ttgcaatcgt gactgcatcc ttgcctttta atgccttttt acacaccttc ccgatcagcc
      481 aggtcagcga tgcatacgcc aatcgttctt ccctgacctg tgccctgaaa tcttcctccg
      541 cccatcttaa cttttcttcc aaagtagcca tattgtctct cttttactct tcttctgatt
      601 ttttcatcga acgatgagcg taaatatgag gtggtagcaa tgcaggctga cataaacagg
      661 ttctgagtat atttcagaat actgagtata gaaaagaaat gtccggaata aaataaaatt
      721 atatacgcag gtgcctttta ggtggctcgg agaagatccg ggctttttta tattcgtttt
      781 caggaggtaa tttcacatgt tcaggaacac aaaaggaaac aggcgttttc tggatttttg
      841 gctttttcta ttaaaaaaga aataagcgac cgtttgaaaa tacttttccc atttttcgta
      901 cctgctgcgg cacaatatgt ttttgctgca gaaaagaaaa aaccacagcc agtcagggtt
      961 taccgactgt ccgtggtctt ttccaattta ttatgacata tgtgtttttg ctctttgttt
     1021 ccgttataat gggaattaac aaatccccca aacgaacctc aaacctcatc aatggcatga
     1081 ccgatatcat ttctcatata tttattccca aattccaccc actcggttgc cttgtaagca
     1141 ttggcacggg cctctttgag atcgcctcct gtggctgtga cgcccagcac ccggccgccg
     1201 tttgtgacga tctggccctg atcgtttaat tttgtgcctg catggaatac aaaatatccg
     1261 tctgcatcct taaaacgctc caggccggaa ataggatagc ccttttcata gtgctccgga
     1321 tatccgtccg atgccaggac tacgcatacg gctgcattgt cctcaaattc cagttccacc
     1381 tgatccagag tgccgtcaat gcaggcttca aaaagatctg ccagatcgtt tttcatcctt
     1441 gggagcacca cctgggtctc cggatcaccg aagcgggcgt tgtactccag caccttgggg
     1501 ccatcttcag tgagcatcag accaaagaaa atgatcccct taaattctct accctccgct
     1561 ctcatggcat ccaccgtagg ctgatagatc ttttccctgc agaatgcatc tacctcggag
     1621 gtatagaagg gacttgggga aaatgtgccc attccgcctg tatttaatcc ctgatcccca
     1681 tcctttgccc gcttgtgatc ctgagcagaa gtcatgattt tgatggtctt tccatccaca
     1741 aaggatagga cggaaacctc ccgtccggtc ataaattcct cgattacgat ctcgtctccg
     1801 gcagagccaa actgcttatc cagcatcagg gtgcgtaccc cttcctttgc ttcttccaga
     1861 ttactgcaga ttaaaacgcc ttttcccaga gccaggccgt ctgcctttaa tacgatgggc
     1921 attctggcag tttccaggta tttgatggcc tcctccgggt ctgtgaaggt ttcgtaagcc
     1981 gctgtgggga tattgtattt tttcatcaga tcctttgaaa atgccttgga accctccaga
     2041 atcgctgcat ttgctctggg gccgaaggca cgcagtcctg ccgcctcaaa ggcatctacg
     2101 gctcctgctg ccagaggatc atccggcgct actacggtta agtcaatccc atgctccttt
     2161 gcaaaggcca cctgccggtc aaattccatc acgccgatat tgacgcattc tgcaatctct
     2221 gcgattcccg catttccggg agcacagtaa attttttcta ctctggggct ttttgaaagc
     2281 ttccatgcaa tggcatgctc tcttccgccg cctcctatga tcagaatctt catatcaact
     2341 cttcctctcc tatacttccc catgggcgat catctggatt gcttttggaa gaataatcca
     2401 ttctgcctgc tccatgaccc gtctctgcag gatttcaggg gtatctccct tttccacctc
     2461 tactgccttc tgcagaatga tcggccccgt atccataccg ccgtccacat aatgtaccgt
     2521 tgcacctgtg acctttacgc ctcttctcag cgcttcctca tgaaccttca gtccataata
     2581 gcctacaccg cagaaggatg ggatcaggga cggatggata ttgatgatcc ttccctcata
     2641 ttttttcacc atagcctcag gaatttttac caaaaagccc gccagcacga taagatccag
     2701 cttatattta tctacttttt caagaaaggc cttgttaaag gcttccctgc tctcaaactc
     2761 cttcggagat atacatacag cctcaattcc gtgcgttctg gcccgctcta aggcataagc
     2821 gccgggattg ttgctgatca ctacactgat ttccgtgtct gtgatcttcc cgctgtcaac
     2881 ggcgtccaga attgcctgca gattagtgcc tccgccggat accattatcc ctgttcttaa
     2941 cataaggcca ctcccttttc gccctcttca atggagccga tcacataagg agtatctccg
     3001 gccgcccgga tggcctccat agccttctct gcatctgccg ggtcaactgc cacgatcata
     3061 ccgattccca tattgaaggt gttgtacatc atttcttccg caatatttcc cttttcctgt
     3121 aaaagcttaa agattgccgg aacctcatag ctgtcctttt taatcactgc acggacgccg
     3181 tcctttaaca tacggggcac attttcataa aaaccgcctc cggtaatgtg gctgcaggcc
     3241 tttacggcca ctccggcctt tttaatactc tttaatgctt ttacatagat acgggtaggc
     3301 gccaaaaggg cttctccaag agtctttccc aggcagtcat aataggtatt taatccttct
     3361 gcggtcatct cctgctcaaa taccttgcgc accagtgaaa aaccattaga atgtacgccg
     3421 gtggatgcca taccgatgag cacgtctcct gcctttaagc tctctcctgt gatcatttcc
     3481 ttccggtcgc agacaccgac tgcaaagcct gccagatcgt aatcatcctc aggcatcagt
     3541 cccggatgct ctgccgtctc gcctccaatc aaggcagcct cagactgtaa gcacccttca
     3601 gccactccct ttacgatgtc ggcaatcttt tcaggataat tctttccgca ggcaatgtaa
     3661 tccaggaaaa ataaaggctc gcctccggcg caggcaatat cgtttacgca catggccacc
     3721 gcatcaatac cgatggtgtt atgcttatcc atgatgatcg ccagcttcac cttggtaccg
     3781 catccgtctg taccggataa aagcactgga tcctccatgt ccttaatctt tgacagggaa
     3841 aacgcccctg aaaatccgcc cagaccgcct aatacctccg gccgcatggt tttctttacg
     3901 tgctctttca tcagctctac ggacttgtaa cccgcctcta tatctactcc ggctttctta
     3961 taatccatca tcatctcatc ctctccttta catctgtgca aggtattctt tataacccag
     4021 tttttccagc ttttccgcct tttttaccac atcattttta aggccctcgg aatacaattt
     4081 aagcttttca agaagccctg aatcagaagc agcaaggatc tttgccgcaa ggatgcctgc
     4141 attggctcct ccgttgatgg ctacagtggc aacgggaatg ccgcttggca tctgtacgat
     4201 ggaataaagg gaatctacgc cgcccaggtc tgaggtcttc ataggaatac cgatcacggg
     4261 aagggggaat acagctgcac acatacccgg cagatgggct gcctttccag ctcctgcgat
     4321 aattacctta aagccctttt cctcggcgct ctttgcccac tgaaagaaga tatccggctc
     4381 tctgtgggca gagataatcg tcatctcata atcaattccc agctgatcca ggatctccgc
     4441 tgcctttgcc ataaccggca tatctgagtc gctgcccatt acaattccta cttttgccat
     4501 gtttcaatct ccttttctaa taataaatat tagcttttct aataagtatc tttttatatc
     4561 ttaatgttac ttataattat aatactaaga aactctggca acgtcaatac tattctttta
     4621 aaaaagaagc ttaaaaaagg gcgcattaca gcgcccggtt taattcctct gttccaggca
     4681 gcacaaactg gccggcctga atcagcagaa tttcacttcc gtctgccttt acagccgtga
     4741 tatcccccag ttcattataa ggaatggtaa tatctgtatg acagctgaaa taggctctgg
     4801 acatatcttc ttttcttaaa agggaaatct cattgtccct ggcaatcact tcttttccgt
     4861 caggattgta catgggtgaa tcctcggcaa agctgtagca ggtatctccc actgcaaagt
     4921 gaggccctgt cttttctgcg atcaggatag gaagcttgct gccgatgttg tatttttttg
     4981 ccatggcata agctgcggtg ttggttccaa tagcgaactc gcctaaggga agccagctgt
     5041 gaccgtttaa gattacctgc tttaccagtt cttttcctga atcgaaattt ccgcaggaaa
     5101 actccgtcac ccttccatcc tcaaactcca gacgaagatc cttaaagaga tactccctca
     5161 catagacctc cttcacatga agcaggccct ttgttccctc taataccgga gaagtaaaga
     5221 cttcccccag aggaatattt acatcggaaa cacagttttc aaagtttgtt tccctggaag
     5281 ggtcatttaa agtgtgcaga tgaacagtca gatcggtttc attaccctcc cgtcctgtga
     5341 tccgcacata ctccgcctga tccaatgcat caataagcgt ctgctggatt ttctggtagg
     5401 tatcgtaatc caaagtattg atccggatag tttccttaaa aatctcctca aaatcaggcc
     5461 cgatctccgg tcttggaaat gcgatgatgg taaaactggt ctcatctccg ggcatatact
     5521 ggttcacgat ctgcctggat gcagtggcat aggaaagagt cagctcctgc tggtgatcat
     5581 ttaaagaaaa cgcagtcttc ttatttactg gctcaaattc ctcttcccca aaggtttcca
     5641 ccacagccgg accggcacac atagctgcca ggtctttata ttcctcataa gcggttttta
     5701 aaatagacag cttgcgctcc ttaaaagcat ttcccatata cagggcgctg tcatagcggt
     5761 ggtcataatc atactggcgg ttggcagacg taccgtaata tccccttttg ccgtttgaac
     5821 ggcggtttaa ggactccact gcggcacgat agcagaccgt ttccagcccc aggctgcgaa
     5881 actgctccac tgcacggcgg atcatgcgct caaatcccag ctggtattcc actaccactg
     5941 tctttttctt tgaaagatcc ctgcccatta cctcaaagcc gcggcggtag ccttctgtat
     6001 aggtatgggc cattttttca atggtttcct ccggcagcga agccataaac gctgccagcc
     6061 tacgctctga atcagaaaca tactctccgt actgatacag atagcgcaga tctgtcaggt
     6121 ctgaatccat aatgatatct gctgcaaagc ttaaagcagg atccagccct tctctgatcc
     6181 gccatggcag atgcagatct acataatcgc tcacatacca gtacagcgca tcccgtaccg
     6241 cctgctccct gggagtctca ttttcctcct cccatgcctg ggtaaaaagg ttataaacct
     6301 ctaaaaaggt ctcgcacaaa atcgcaatat attccaggcg catttcaaag gcatacacaa
     6361 tgcagcccct gagttctgta taaagaaagg ataaaagagg gccaaattcc ttccccagac
     6421 agtctgccgc ataatcagga ttggcaaagc tctccccata attttcaggg agaatatcct
     6481 cataaaggcg gtgattccag tctgaaagct cctctaagga aagcttctcc cagctctccg
     6541 gcagttcttt ctccccaatt cccctggcag ttaaaagctc acggatcatg cctaagaatc
     6601 cggcggtccg tttaaaataa aggccgtatg gcagacgagc atcctcctcc ttaatcgaac
     6661 ggatacgatc cagagaaagc tccaggcgtt cccttacggc ttcattttct tcccgtaata
     6721 atattctgta atccatagaa aactcctact ctaccgtcct gtaaatccta tgataagaat
     6781 cacagcccag ataataataa gaatgccctc cagggcgatt ccgatcttag ccaggatata
     6841 gttgcagtta tcctccgtaa atgaaaggat accataccac aggcctgccg cagcagccac
     6901 aatgctggaa aacccgtagg ctcctgtatt taatcctccc tgtccggctc cctttaagga
     6961 aagataaatg ctgaaagttc ccagccctag tgccaggatt cccagtccca gacagaagaa
     7021 gctctgtctt gcaaagggat ttctcacata ggatatttta tttcccacct gtctcaacgc
     7081 ttccacctcc tgtaatggat gagaaccctc acataatgag cgattccatg aatataaaag
     7141 ccaataccgg ctcccagcgt attcaggaac atatcgtcca catcaaagct tcccacccgc
     7201 aggatcagct gaagggtttc taacatcagg ctccatataa agccgaacat cactgtatta
     7261 taccacaggc ggcttctttt gctgataatg ggcagaaaga aaccaaaagg gataaaggct
     7321 gcgatattac ctacaatatt gatcataaaa aggccgaagc cgagaacctc ataataacgg
     7381 aaataacggc cgatttcttt aaaaagcacc agattgtagg catattcccg atccgtttcc
     7441 gttcgtccga aggattctga aaaaaacata aagtaggcca gaaggcagag atacagtata
     7501 aacagtaccc atcctgcctt ctggcgcggg gtggtatgtc ggatcatcac agcccgcctg
     7561 ttaaaattca atctcggatt tgcgctttaa aagcagcgca ccgtttacga ttgaaataat
     7621 tcccacagcc agtcctgtca ctgcaatgat gatacccaca gcaatacagc cggcgccggt
     7681 attgcgcatg gtcttatata ctctttccat atttttactg cctttcatgg tttgtacagc
     7741 caggttccat tctaagcgaa tggttacgcc tgcgctccat actctgcata gtatgcgtca
     7801 atggctgcct ttaatgtatc attgatcaca atttttccac cgcattcctt attgtacagg
     7861 tattcggtca catctgccat ggtaacaatg gctgctgtgg ggaaaccata gagatccttg
     7921 atctcgtcta aagcactctt tgttcccttg ccacgttcca tacggtttaa agatacgatc
     7981 agacccttga tctccacatc gccctgagcc tttaagatgg ggaaggtctc ctcgatagac
     8041 ttaccggagg tggttacatc ctcgatgatc atcacacggt ctccgtcctt aataggactt
     8101 cctaaaagaa tacctgtatc gccatgatct tttacctcct tacggttgga gcaataacgg
     8161 atatcctttc cgtaaagctc actgatcgcc atagtggttg ccacgcttaa ggggataccc
     8221 ttataagccg gtccaaacag cacgtcaaaa tccaggccaa aattgtcatg gatggccttt
     8281 gcataatatt cgcccagctt acgcagctgt gttcctgtca catatgctcc tgcattcata
     8341 aaaaacgggg acttacgtcc gctctttaat gtaaaatctc caaacttaag tacattgctc
     8401 tctaccataa attcaataaa ttcctgctta tactgttcca tgtaataaaa cctccttcat
     8461 ttcttctacc taattttacc atagattgcg ggcattgtga actacctatt gtctgaagcc
     8521 aatgaacttc ctgcttcata gacctcgctg ccttacccca ccgtctaaag ccagtgggat
     8581 tgcggcagcc tcatttcaac ctcccagcat aaatttctct acgaccagcc ccaccccgtc
     8641 ctcattgttt gaggcagtga tatagtccgc tgcattgcgc acaggaagaa ccgcattttc
     8701 catggccact cccagaccgg catactggat catagtcagg tcattatagc cgtctccaca
     8761 ggcgatcatc tcttcccgtt tcatgccgat ccgctctaaa agccgcccca aagactgggc
     8821 cttgtcaatt ccctttggca taacctccag gaaaaatggc tctgaacggt agacactgaa
     8881 atcccggccc atggcagcct ttacccgcgg ctccaccgtt accagataat ctccgtcatc
     8941 cagcatcaga aactttggaa ccggaaagga cacatagcct ttcatgtctt ctacctggcg
     9001 cagaggcagg tgattgatat tggattcctt gacggcatag ggacattcct cattgtttgt
     9061 gatgatctcc tccccctcat aggtcaggat atccactccg tactcctctg ccagaccgat
     9121 gattttctca tttgaatcca cgggaagcag ggaggaaaat accacttccc cggtgccgca
     9181 gtttgtgatc ataccgccat tataggataa aatatagctt ccgtacgctg caagctcaag
     9241 ctcctctgcc agcggcttga tccctgctgt gggccgcccg gaagccagaa ccacgatctt
     9301 tcccattttc tgggcttcca taagagcctg tttcgttctg ggagtaatga ttttatccct
     9361 gtttgtgagg gttccgtcca gatccaggac gatcatttta tattccattt tataaattct
     9421 cctgattttt catttaaact gccggttgaa accgctgtat ccgttttatt ttaccagata
     9481 accatctata agaaaagccc ggattcaccc ttgagtttcc gctttttgca gtgtgacctg
     9541 gggtctggtg tgcagggcga ctttgaaggt cattagaaaa ccttagaaga ttaaattttt
     9601 tgttaaaatc catatcaaac ctgtttgagc gcagcgagtt tttgatatgg cttttgtttt
     9661 tagaaaaatt tcagactctt aggctttctt atgaccggaa accggagccc tgcacaccag
     9721 accccagggt gcggtgcaaa atgcggaaac tcaaacggat tcaccctaat gcaaaacatg
     9781 caatacaata acagtataaa accatatcca aggagattcc attatgtatg caactcatac
     9841 ccgaaaactc tggcctcttc tcacagccgg aatccttgct gcgctgtccc tctctggctg
     9901 ccagtcctcc ctctccccag acggacagac tgccgtcacc ctcagcgaag tagcccactc
     9961 catcttctac gccccccagt acgctgccat tgaactgggc tattttgaag aggagggcat
    10021 tgatctgact cttgtaaatg ccggaggcgc tgacaaggtg atgacctcct taatctccgg
    10081 agacgcccag atcggtttta tgggttcaga agccagcatc tatgtttatc aggaaggttc
    10141 ggatgatcct gccgtcaact ttgcccagct gacccagaga gccgggaatt ttctggtagg
    10201 aaggcaggcg gaggaagatt ttaactggca ggatctgagg ggtaaaacgg tgctgggagg
    10261 aagagccggc ggtatgcccc agatggtatt tgaatatatc ttaaaaaaga acggtcttga
    10321 tcctgttaat gacctcacca tcgaccagag catcagcttt ggcctcaccg cggcggcatt
    10381 taccagcagt gctgcggatt ataccatcga attcgaacct ttcgccacaa cccttgaaat
    10441 ggagggaagc ggtgcggtgg tggcgtctct cggaacagaa tccggctatg taccctatac
    10501 ggcctacagc gccagaaaaa gctatctgaa gcagcacccg gaggtaatcc aaagctttac
    10561 caatgccatc cagaaaggtc tggactatgt aaacagccat tcctctgaag aaattgccaa
    10621 ggtcatttcc ccgcatttta aggaaacgcc ggtggataag ctggccctga ttgtagatcg
    10681 ctacaaatcc caggatacct ggaaggagga tacggtcttt gaagaaaaca gctttacgct
    10741 gttaaaacgt attttggagg aagcagggga attaaaggca gaagtcccct ataacgaact
    10801 tgtgacaatg gatttctcca aaaaagcagc aaaaaattct caataacacc cttgacaaat
    10861 actctctatt catatataat tcccctaatt accactgtat cacattacta cgctaaagtt
    10921 ttagcatcat ccttatgcta aagccttaca aaaccgctaa aagggaggat tattatgaaa
    10981 aaaacagttc agatgttaat cgttctggcc tctgcagccg ctctgactgc cataagcggc
    11041 tgctctgcca taggttctgc ccccactctg ccatcagaaa caatccctga taccatatcc
    11101 gcttcctctg aagctgccaa agccgcctct gaagaaacca ccggcagcaa tggaggaagc
    11161 cgcctgaacg atatcctgac ccgcggatat attgaggtag ccatggagcc ttattttgct
    11221 cccaatgaat ttatcgatcc ttccagacaa ggagaggaac agtacgttgg agccgacgtt
    11281 gagctagccc gttatattgc cgacaagctg ggggtagagt gccggatcgt tcctctggaa
    11341 ttctccgcag tcttaaccgg cgttaccgaa ggcaaatacg atctggcgat ttcagctctt
    11401 gcctataccc cggcccgcgc agaggctatg gaaatgtcaa aaggctaccg ttttgatgag
    11461 gataacgcca acaattttgg tctgatgatt cgcagcggag atctggacac gatcaaaagc
    11521 gctgatgacc tgaccgataa aatcgtagtc tgccagagcg gctccctgca ggagctcttt
    11581 gctacagagc agattccggc ctgcagggag ctgaaacggg tatctgctac caccgacggt
    11641 tttttaatgg ttcaggaagg aaaagcagat gcggttgtga ctgaaaaaaa gacagcagag
    11701 ctttttatcg cagccaacgc ggaaagcagt atggtcattg ttcccgattt ttccttcgtc
    11761 gttgacgaga gcacttccgg aacgcggatc ggaatcccca aaggcgaaac agctctttta
    11821 aacaagatca atgagatcat tgatgaggtc acagcctcag gcatatatga cgaatggtat
    11881 gagaaataca cagaatatgc aaaaactctg ggattatgac atcctgctcc ctgcagggat
    11941 acacataata aagaaaatga aagaaaggtg acttccgata tgtgggcaac gatcatcaaa
    12001 atgtggaatc gatacagcta tgtatacata cagggactga cgggaacctt gtgtctggcc
    12061 gccatcaccg tactgtgcgg caccgtactg ggcaccctcc tggccatcat gcggcgttcc
    12121 cgcatcaaac ccttccagtg gttcgtacag ttttatgtct gggttctcag gggaacacca
    12181 atccttctgc agctctattt tttctggatc ttccttccca gagccaccgg gattaaaatt
    12241 cctgactcca cctgcatcat catcgccctg atcgtcaatg ccagctctta cgtatcggaa
    12301 atcatccggg ccggcatcca ggccgtagat atcggacaga cggaggcagc ccgcagcctg
    12361 gggatgaccg cccggaatac catgactaaa attatccttc ctcaggcagt taaaaatatc
    12421 cttcctgccc ttggaaatga atttatcacc atgattaaac aggcttccct ggcgtctgtc
    12481 ttctttatca atgaactgac cacctcatac aaaaccgtag cctccgctac cttccggcag
    12541 gtggaatcca ttatcattgc cggcatcatc tatctgctgg tgaccacaat actcggccgg
    12601 attatggctt atgctgaatg gaggatgaaa caaaatgaac gctgaaaaca aaatctgtgc
    12661 cgaagcccgg cccatcatcc aggtaaacaa tctgacaaaa tcctttggaa agctggaagt
    12721 cctgaaaggg atttctgaaa ctatttacga aggcgaagta gtctctatca ttggtcccag
    12781 cggcggcgga aaaagcacct ttctccgctg cctgaatctg ctggaaaccc cggactccgg
    12841 caccatcctg ttccgaaact ccaatatcac ggatcgttct gtcaacgtag accaatatcg
    12901 tcagcatctg ggcatggtct tccagcattt taacattttc ccccatctga aggtaataga
    12961 caatgtcacc ctcgccccgg tgctggaaaa aaagattcca aaggcgcagg caaaagccgc
    13021 cgctctccgt ctgctggaac gggtcggtct ctcggacaaa aaagacgaat atcccaaccg
    13081 gctctccggg ggccagaaac agcggctggc catcgtccgt gctctggcga tggagccgga
    13141 gatcatgctc tttgacgaac ccacctccgc cctggatccg gaaatggtaa aagaggtgct
    13201 gaacgtaatc cgggatcttg ccagctccgg catgaccatc ctcatcgtca ctcatgaaat
    13261 gggctttgca cgggaggtca gcgaccgcat cttcttcatt gcagacggca ttgttcagga
    13321 aaagggaagt ccggaggaac tgttcctgga tccgcaaaac cccagaacca gagaatttct
    13381 gggaaaaata ttataagtat atggaaggag tacctatgaa aaccttggag tttatcgaaa
    13441 agattatcga agaaaaatca acccgtatca tcgaggccaa cgatcaaatc tgggaatttg
    13501 ccgaactggc atatcatgaa tatcagtctg ccgggctgct ctgccaattt ctgaaggatg
    13561 aaggttttac ggtagaaacc ggtctggccg gtatccccac ctgttttcag ggaacttatt
    13621 cctatggatg cggaaaaccg gtcgttggta ttctgggaga atatgacgcc ctttccggtt
    13681 taaaccaggc ggctgcctgc ccgtccagac aacctatcat ccagggagca cctggccacg
    13741 gatgtggaca cagtgccctg ggtgccggag ctctggcagc cgccattgcc gtcaaggaat
    13801 atctgaaaac cttcaaaaaa ggcggcacag tcatctattt tggctgtccg gcagaggagg
    13861 gggccggttc caaacagttc atggccagag ccgggctgtt cgatcaggtg gattttgttt
    13921 atacctggca cccggactcc atcaatcagg tggacaactc ccacaataac gcgattatgg
    13981 gagccaattt ccactttaaa ggccttactt cccacgccgg ctctgccccc tatctgggcc
    14041 gaagcgccct ggacgccgca gagctgatga gcgtaggctg caattatctg cgggaacaca
    14101 tgattccgga agcccggatc cattatgcct acattgacgc cggcggcact gcccccaatg
    14161 tggtacagga ccgctccacc gtccggtatg aagtgcgttc cccttatgtc cgccagctga
    14221 cagagctgtt caaccgtgtc gtggatgtgg caaagggtgc cgcccttatg accggcactt
    14281 ccatgaatta tgatctgtct atggccttta cggaatacct ccccaacaaa gcccttgccc
    14341 agatcgcaga cgcctgtatg caggaggtag gcgcaccaaa atggaccgag gaggactacc
    14401 ggctggccag acagtttttg gattcttatg atgatactgc ccggaaaatg atccgtgacg
    14461 aaatcattca gatctatgga gaagaccggc ttgaggaaat tctgtccaaa cctttagaca
    14521 gtgagatcca tcctttcgat cccaaccata tcatccagac tgcaggctct accgacgtag
    14581 gcgatgtggg ctatgcagtt cctactctaa atctgcgggt tgccgcctgc tgtgtgggca
    14641 atgtggggca cacctggcag atgacggccc aaacctgcag ccccattgcc cataaaggac
    14701 tcctgacggc cggaaagatg attgccctgt ctgcgatccg caccatggag cgccctgaca
    14761 tcattgacgc cgccaagaca gagactctta aaagaaacag cggtcattat acctgtcccc
    14821 ttcccgatca cgtgcttcct ccattagaga catattaaaa gtcaaaagct ttctcaagcc
    14881 tccatatttt tatcatccat ttcagataat ctccatactg cgcctcccgc accgcctctg
    14941 cggtcactac atccatctct ccgatcttct ctccgtttaa cagatattcc agcaccccag
    15001 ccttctggcc cggctccacc ggagccggaa gggactcgga gagaacaagc ttcttttcca
    15061 cagatgaaag atcttctccc ttaaggctga gatatctgaa tgtaccgttc aaaaccaact
    15121 ctgccccgct tttaattcct ccttctaccg aaatttccgg aagtgtgatc ccctctttat
    15181 cctcataaag cttacagttt gcatagccat agtttagcag cgtctgtgcg tctgcaaaac
    15241 gtgctttgaa atcaggagca gccatgatcg tagcgatcag gcgcacccca tccttctctg
    15301 ccgtggctga cagacagtat tttgccagag aggtggagcc tgtctttaag ccggtaacct
    15361 taaaattcgt tgccatccgc aaaagtttat ttgtgttgga aagaccaaat tcctttgtcc
    15421 cctgttttgt cacatgagta atattttcca tccagattgt tgaataattg tggatctgag
    15481 gatactgatc gatcagctcc cggctcataa gagcaatatc tctggcagtt gtcacatggg
    15541 agggatcctc tgtaagaccg cagcagtcca caaaatgggt tttcgtcatc cccagacctt
    15601 cggcacgttc attcatctgc tgtacaaagg cctcctctgt accggaaata tactccgcca
    15661 tggctacaga agcatcattt cctgatgcga tcacgatgca tttaatcagt gtttccaccg
    15721 tctgcgtttc cccttcctcc aaaaatacct gggaaccgcc catggacttg gcatgagcgc
    15781 tggtaactac ctcatcggaa aggtggatct tcccttctgc cagggcatca aaaatcagga
    15841 tcagcgtcat gacctttgta acgctggcag gacttctttt atcatccgcc cctttttcat
    15901 atatgatctg ccccgtagat gcctccatga gaacggctga aggcgctgat atctggattg
    15961 ccgtctgaga agctgccgca tctcccgctt ctgcctgtat ctgcgcagtt tctctgctta
    16021 ccgctctggc cggcaccccg gccggaagcg caagctccgg ttcagaagcc aatgctgccg
    16081 tcacctgccc tgccgtcata agaagcgcaa gcaaaaagcc tgctgctttt gtccgtttca
    16141 tacacgattc ctccaaacat tgctgctctc tgcctaatat atgaaaccgg acaaacgctt
    16201 tatgtcgcat caaaaaagac aaaacccccg gctgcggctt ctgccgctgc cagaggttat
    16261 tttatactat gtgttcctat acttccttta aagcctgtct taaggcctct gccatctcct
    16321 catactcagc gtctgtgaga atggttcttc cgctgttcat ctcaaaggta ccgccccact
    16381 cttctccgcc cttatatttg ggaacgatat ggaaatgaag gtggcagcct gtgtcgccgt
    16441 atgcgccgta gtttacctta tcgggatgaa ataccttgtg tacggctctt gccacctgag
    16501 ccacctctgc aaagaaatca tttctctctt tatctgttaa gtcgatcagc tcgctgacat
    16561 gctttttgtg agccaggatc actcttccct ttttactctg ctccttaaat acataaagaa
    16621 agcctgtctt catctcgcag accggatagg caaattttgc taccagctct ccctgcatac
    16681 agtatgcgca atttggatct tttaccattt ctgccataat tttatctcct tttcatagcc
    16741 ggctctgctg tgtcaaaaag cagccgccgc ttttgggtaa tttctatttt aaacatacca
    16801 cggcttccat caaatttcaa tcactttcat ttcctgaatt ttatgttata attaaattga
    16861 tacaatttta ctttcattca aacaggagat ttacaactta tgagccgaaa tgatttcaga
    16921 cgccggcagg cccaaagaca gcgaagaaag cttgtccggc atcttcctat tatacttgca
    16981 ctgtttctgg cgttttcagc cctggcagca ggcgtttttg cactgacccg ctttgtctta
    17041 aacagacagc ccatatcatc tgcttccgcc tcctccagta cagccaaaac aacctccgaa
    17101 gatccggcac agcctatctc ttccccggcc tctggcacac ttgctcaggc cgcatatatc
    17161 gctgcgggct atgactacga ccgtgccatt gctctcatta aagaagactc ctctctggca
    17221 gaagaccccg ctgccctgga agcgatcaca tcttatgagg aagcaaaggc ttctctctta
    17281 ccagctgatg tgcagaacgt tccccatgta tttttccatt ctcttatcat ggacactgcc
    17341 aaagcctttg acggcgacag ccggactccc ggctacaatt ccgtcatgac tacaaaggat
    17401 gaatttttaa agatgctgga tgctttctat gaggacggtt atgttctggt acgcatccgg
    17461 gatattgcct ctgagactac agatgaaaat ggaaataccg ttttctcctg gggagaaatc
    17521 ctgcttcctg agggcaaaaa acctctggtc atgtcccagg atgacgtatg ctattatcct
    17581 tatatggaaa aggacggctt tgcacgccgg atcgtcatcg gagaggacgg aaaacccact
    17641 gctgaaatgg tcatggaaga cggctccatt tccaccggcc cttatgacct aatcccgatt
    17701 ttagaagatt tcatcgaaga acaccccgat ttttcctata aaggtgccag agcgatcatc
    17761 gcctttacgg gctatgaagg aattttaggc taccgcactg ctgcttccta cagtgacagt
    17821 ccgaactatg aacaggatag agagcaggct gcaaaggtag cccagtgttt aaaagacaat
    17881 ggctgggagc ttgcaagcca cagctggggc catctgtgga tgggcgtttc ctccgtaccc
    17941 ggagaaacct tcaagatcag cgacgagcgc ttctatacag atacggacaa gtgggaagca
    18001 gaggtagaat ctcttatcgg gccaaccgat atctacatct atcccaacgg caatgatgtg
    18061 gcagactggc ggccctatac agaggataat taccgtttta aatatctcca ttccaagggc
    18121 ttccggtatt tctgcaatgt ggacgcaagc aagcccgcct ggatccaaaa gggaagcgac
    18181 catctgcgga tggccagacg gaatctggac ggctaccgtc tttacgagga tatgatccag
    18241 acggatccat ccaaaaaacg gctgtccgat ctctttgacg cttctcagat ctttgatgcc
    18301 tcacggccca ctcccgtgac ttggagctat acacagaatg aagcggccgc agatgcctct
    18361 gccccggcag caccggaaac agttccatcc gaaagcgctg cttcccagta aaccagatct
    18421 gaaccatcct gtctcctttc gcgtatacta catcaaagac gtacttaggt gaaaggagat
    18481 tttacaaaat gacacccaaa ctggatgtaa acggagtcag cttttcctac catacgctgg
    18541 aaggagaaac gctggctctt tctgatatat cattttccct gtctcccggt gaatttgcag
    18601 ccgtggtcgg cccgtccggc tgcggaaaat ccacccttct ttctcttctc tgcggtttgt
    18661 ccaaacctga tacggggcag atcctcttag acggccatcc gctgcgccaa tcgccgtctg
    18721 ataccggcta tatgctccaa aaggatcatc tttttccgtg gagggatatt ttatccaatg
    18781 tatctctggg actggaaatc cagaaccgcc ttaccccaaa gacaactgcc cgcttaaagg
    18841 aaatgctctc agcctacggc cttgaaggct ttgaacacgc ccggccctct gagctttccg
    18901 gaggaatgcg ccagcgggcc gctctgatcc gtactctggc gctggaaccg gatcttcttc
    18961 ttttagatga gccattttct gctctggatt accagacacg gctttctgta tgtgacgata
    19021 tcagttctat cttacggagc actaaaaaaa cagccgttct tgtgacccac gatctttcag
    19081 aagccatcag catcgcagac cggatcatca tactcacacc ccggcccgga cggatcaaaa
    19141 aaatccttcc catccatttc ccggctgact gtgcaacacc tttaaaaaga cgtaactgtc
    19201 ctgaattttc cggatatttc aacgctgtat ggaaggagct gaaaacagat gaataatccc
    19261 ttactctccc cctcgttagc ccagcaggaa tacctggccg accagctgcg ccgacggagt
    19321 atgatccgta tctggcgcat ccttcttctg attctgattc tgtctttatg ggagctgtca
    19381 gccggcttag gtctcatcga ccggtttatt ttcagcagtc caagcctgat cttcaaatgc
    19441 ctgatttcta tgactgccga cagaaccatc tttctccaca caggcgttac cctcatggag
    19501 acgcttataa gctttgctgt ctgtaccgca gccggtctgg tctgtgccct tcttctctgg
    19561 gccagtccct ctgctgccag cgttttagag ccattcttag ttcttttaaa cagtctgccc
    19621 aaatccgctt tggctccgct tttaatcgta tggctgggaa ataataccac caccattgtc
    19681 acagcggccg tttccgtggc tctctttggc tcgatcctta ctctgtacaa tggtttctgt
    19741 cagaccgatg aagacaaaat acgcctgatc cgttcccttg gaggaaccag aacagacgta
    19801 ctctttaagg ttcttctgcc aggctccctc cccatggtga tcaataacat gaaggtcaat
    19861 atcggcctgt gcctggtcgg cgtcatcatt ggtgaatttc tctccgcacg ggcaggactg
    19921 ggatatctga tcacctactc cgcccagacc tttaccatga cggtgcttat gacctccatc
    19981 atgattctgt gcctgctgtc ctggctttta tatcaggcca ttgcctgtgt ggaaaagaag
    20041 ctgacacgat aaaatgcaaa atgcaaaaac tcaaaaaccg gtctgcttgc actcccattt
    20101 ctcctatgat atgatgaaac catctgatta aagcagagca tttccgccga taaagcccgc
    20161 aaaaaggaga aatcaatgga cctgatcaac cagtttattg acaactacaa aaagaaattt
    20221 aactactatg aatctgtagg ccgtcttgca gccggtcagc tggaggctgt gctgcgctcc
    20281 tccggtatcc gggccatggt tacctaccgg gccaaaaatc cgggacgctt aaaaagcaag
    20341 gttctccgcc gcaatgtcaa gcgcccggtt ccttaccgga atataaagga aatctatgag
    20401 gacatcgccg atctctgcgg tgtccgggtt tccctctatt tccccggaga ccgccttcag
    20461 gcagaccagc tgatccggga tcagttccac attctggaaa gcaagcagtt cccggagcag
    20521 tcaaagcctc ctacttatca taagcgtttt tccggctact gggccaacca ctaccgtgtc
    20581 tatctgagag aagaattggt acaaccctca gaaaaacgct actgccgggc cagaatcgaa
    20641 attcaggtag cgtccgtcct gatgcacgcc tggtctgagg tagaacatga cttaatctac
    20701 aaaccccttc agggtacgct ttccaaagaa gagcttgcca tcctagacga gttaaacggt
    20761 cttgtccttg caggagaaat cgccctggag cgcctgcagg ctgcaggaaa tgaacggatc
    20821 cgcagcaaaa atgctgtttt taacaaccag tacgagctgg cttcctatct ttacaattac
    20881 ctcagcagca atttccgccc ggaggatatc gaactgcgga tgggaaatat tgagctgctc
    20941 ttccggcttt tgtccagcct gaagctgctg gacgtaaagt acatgggccc tattttaaag
    21001 gcagttaaat ttgaaaagga caaacggaac atttcccagc agattattga ccagatcatc
    21061 agcggaaatg aaaagcggta tctggcctac cagtccttaa aggcagccgg ctctaaagag
    21121 gatgaggaac gtctgaaagc catcagcgcc tttttcagtc aatgggttcc cctggaagcg
    21181 cttttaaacc gcaccaccaa gcgcagctcc cgttccttaa gcgtctttaa tatcaacgcc
    21241 ctgaaacgga ccgggctttt tgacaaagac agcctgaatc agattgcatt tcttagaaaa
    21301 atgcgcaata ccctgatcca tgatattgag gttccggaaa tctcctgcat acgggagctg
    21361 gccgaacagg cccgggctct gaatgaaact cttttaaaac tccttgagcc ggaaaaggac
    21421 gaggcagact aaaggttatt atcccaaaag ctcccggatc atttttccgt caaataccac
    21481 cttttcaaga tgatccacct cgatctgata cagggcattg gcagcgatat gctccgcagc
    21541 ctgctccaga tcacggattc cgctcgtatc tgaataaagc tccactacgg tatcaagagc
    21601 ctcttcggtc aggatacact cctctttgcg caggctcatg cggcgcagga tctttggcag
    21661 agcaaatttt gagaaaatga tctttttttc ttccaatgta tagtccggaa tatcgatcac
    21721 cgcaaaacgg gacattaatg gtgcgctgat ctgctcctta ttgttagctg tggcaatggg
    21781 atatacgcct gaggtaggca ccatacattc tatgtagtta tccgtaaagc ccagattgtc
    21841 aagcagggtt aaaagcacat ctgccggatt tccatttcct tttccggaag ccgctttgtc
    21901 cagttcattg atgataaata caagattgga ctctcctgcc atggaaaatg cctccatgat
    21961 aattcccggc tttgcgttgg catagactct ggagcttcct gtcagctgct ccgggtcatt
    22021 gatggagctc atatccagag ttgtccacgg cagctttaag attcttgcca ccgcataagc
    22081 gatctgggat tttcccgtac ccgcaggccc caccagcaaa aggccgtatg cgggcagcgt
    22141 gtgagttcgg ttgatctgga taatggtttc tataatcctc tgctttaccc gctccatgcc
    22201 gtaaagctct tcatctaaaa tccgtcttgc ctctaccgga tcaatggctt caaaataatc
    22261 atgcttccac cgcacgttca tcataataga cagcgctctc tgagcatggc ggcgctcctc
    22321 cggggatacc tcatgtgact ttgccactgc caggtttctt cttgcccaga gacggatgtg
    22381 atccggaagg gtctttcctg cacaggtcat aaaatccgta atgctctgga gactggtaag
    22441 cttcatatca tctccttctt cagtttcctc ttcctcctct gtctcagaag gcgcactgct
    22501 ggcaaacaga cgctcaatca taaactgcaa aaagccgtcc tctgctgaaa gcttggttcc
    22561 tcccccaaag gctgtttcac ggatgcggta aacggaatag ccttcctccc tgttctctcc
    22621 ggacagacgc acattgatat ggttttcccc cagccaccgg atcaggctta caaaccgggc
    22681 gtctattttt aaaatatttc cactgaccgt ttcgcctcca tccttaaact caaaagcagt
    22741 tccccttaca ggattggtaa agagaccgca ggtgtgatgc tcccactgct gtccttttgt
    22801 gtctaatacc agaatcggct gctccggcgc tgcctgagga gcatccgaat aaaaaatact
    22861 gtatgtacac tctgctccct ttgtatcttc cgtttccggc gtatggttaa aatcaaacac
    22921 tggcataatc aaattcccct ttttgtacaa agatctacgg ccaggaatct tttccaaaac
    22981 cgtgcctttt tagtgtaaca tgaaatcgct ccgccaacaa ccggcggagc gattttcttt
    23041 atacatcctt tatgcatgtt tcattttttc tattttccaa ccgtcttcca gatttctttc
    23101 ctcaggctca tacgaagact cccgaggcgg cataagtcct tcgaagaaat gatccagcct
    23161 gtcaaacaga cggccgccta ttttatacag tacagccaaa gcacccagca gaataatttc
    23221 tttcatcctc ttcacatccc cttacttctg cttttttatt tcattatacc ctgtgctgta
    23281 taaaagcggc ataagtatcc ataatgcctg tattaaggtt gtataaatat ccctttttct
    23341 tctatatcgt ttgacataaa gcaaacttta ccattataat gaaatgcagt acaaaagaaa
    23401 gggttaaaaa ggataatcat gatcgaattt attacttccg taaacacagc tgtaaacagc
    23461 tttatctggg gagtaccggc catgatctgc atcatcggag taggactttt cttaagcctt
    23521 cgcacccgtt ttatccagat ccgccgcttt ccctatgcca tccgcaccac tctgggccgc
    23581 atgttcagca aaaaagatgc ctccgaaggc gccattaccc ccttccaggc agtctgtact
    23641 gcccttgcgg ctactgtggg tacgggaaat atcgccggtg tagccggagc tattgccatc
    23701 ggcggtccgg gcgctgtatt ctggatgtgg atttccgcat ttttaggaat gtgcaccaaa
    23761 ttttccgagg ttactctggc ggttcatttc cgggaaacta atccggacgg cgatctggcc
    23821 ggaggtccca tgtactatat caaaaatggg cttgggaaaa aatggatgtg gctcgcctat
    23881 cttttttcca ttttcggtat ccttactgta ttcggaaccg gaaatgccac tcaggtcaac
    23941 accatcacaa cggctgtccg gtctgccctc acaggctacg gcatcctgcc tgactctgct
    24001 gtcggtacct ttaatctggc tatgggcatc cttctggctt cctctgtcgc tctggtgctc
    24061 cttggcggca tcaagcgcat cggaagtgta gcggaaaagc tggttccttt catggctctg
    24121 ctctacattc ttctgtcttt tggcgtggta tttttccatc tggaaaatgt tcccggcgta
    24181 ttttcctcaa tcatccaggg agcattctct ccttctgctg tcaccggcgg agtggtagga
    24241 agcttcttta ccagtatgaa gcgcggcgtt tccagaggca tcttctccaa tgaggccgga
    24301 cttggtacag gctctattgc ccacgcctgt gcggatacag ataatcctgt ccatcagggt
    24361 atgtttggta tttttgaggt atttgcagat accattgtga tctgtaccct tactgctctg
    24421 gtaattctct gcagcggtat tcccataacc tatggacagg atgccggagc agagcttacg
    24481 atcctgggct ttacctctac ctacggaaac tgggtgtcca tttttaccgc gatcgccatg
    24541 tgctgctttg ccttctccac cattttaggc tgggggcttt acggcgcaag atgtgtggaa
    24601 tttctccttt ccgcagcctt tataaagccc tttatggtaa tctacgctct ggtatccatt
    24661 cccggagcta ctatggatct ggggcttttg tggagcattg ccgatacctt taacggcctt
    24721 atgtccattc ccaacctgat tgctgtcttc ctgctgtccg gaacggtaat tcggctgacg
    24781 cgggagtatt tctcaaagaa tgcataaacc gtacagcaac ccatccgcca aaaaacataa
    24841 aaacggcagt accgtaagac ttcctttcaa gtccgtccgg cactgccgtt tttattttca
    24901 tcctactgcc cgttatttaa aacctccaca gcctttttaa gctgattgtc atcctccggg
    24961 cggatcactg cctgcgttct taaggattca tccaaaggaa cctccacatc cggcacaatt
    25021 cctttttcat gaaggtcaaa accgctcggc gtataatagt gggcggtcgt aagcttaatg
    25081 gcagaaccat ctcccagagg aatgagattc tgaacaattc cctttccgta gctggtaatt
    25141 cctaccacga ctgcccttcc ataatccttc atcgcacctg taaacacctc cgaagcgctt
    25201 gcagaatcac catttaccag aatggcgata ggaagatcca ccgcatggcc atcggaagcg
    25261 gtataggttt ccccttcccc gtttcggtca gccgtataaa caatgagcga ttttccctct
    25321 cccttcttaa aatccgtcat atcatccggg atcagatagt ccaccatggc tacggcgctg
    25381 tctaatatac ctccgggatt attccgcaga tcgatcacca gctgcttcat cccctctgcc
    25441 gtcaggtcat caatggctgt tttaaactga tctactgtaa tcagatcaaa ttcgctcaca
    25501 gccacataac ctgttgtttc atccagcatc cgggactcca cagtaggaac ctctattttc
    25561 tttctggtaa gagtcagatc cacataggcg tcctcgtcta cccggtatac ggtaatctgc
    25621 accggagttc cttcttctcc ctttacataa ttgctcacga tcacatccag gctttcccct
    25681 gctactaacg tatcttctac cttatagacc acatcccccg gccgcatccc tgcctccaga
    25741 gccggagagc cgtcaaatac ctttaccacc gtacaaaggc cggtggtgcg gttctggctg
    25801 atcatagccc cgattccgga ataggtgccg ctggtgcttt cctgcatcag cttataatcc
    25861 tcttcggaat aatatacaga atagggatca tccaaacctg ccatcatacc gcggtagata
    25921 aaatcctcta cattctgagc gtcctcatca tagagaaata cctggtctat gatctgctgg
    25981 atcatctcca tctttgaggt cacctgctca aagtccactc tctttgcagg ctcctccgta
    26041 atagagggag cgccgggctg aggctgacgg ctgatcaccc gccgtccgaa cagatagatt
    26101 cccgcagaca tacctacgat gatcagcccc acaaacgcca tgacgagagc tcccgtaaga
    26161 actcccatcc agaaccggtt ctggcttcct gccgcctttc ctctctcgct ttgtctgtca
    26221 tatctttgat cttccacttt ttgtctccta tctggccttt aaggggcctc tctgtcctgt
    26281 tacactgtat gcccctttat ggacttacat aaccggaagg atttacatag cttccgccgg
    26341 accggacgcc aaaatgcaga tgaggccctg tggaatatcc tgtcgatcct acctttgcaa
    26401 tagtctgtcc ctgttttaca gtctcccctt ccttcacgag aagctgagag cagtgcatat
    26461 atacagtagt aagcccgccg ccatgatcga tcataatata atttccggcc gaatagctat
    26521 atgtagagat cactaccttg ccatctgctg ccgccacaat agaggaaccg gacggtgccg
    26581 gaatgtcaat tcccttatga ttggtagaag ctccctcggt cggtgagctc cggtccccaa
    26641 acgcggatga aatccgtccg cttgcaggac agggccatat aaaattagtg ttcccttttt
    26701 ttaccgtact atccccaccg gcagaagact gatttttctt ggcttcttct gctcttctgg
    26761 cctcttctgc tcttctggcc tcttctgctt ttctggcttc ttcttccttt ctccgtatct
    26821 ccgcctccac acgggccata tgctcctcct gggctttgat atccgcatta tactggtcaa
    26881 tctctccctg tgcagaagcg atcttcgtct cataggaagc cagctctgcc tccttggaag
    26941 ccatcagtgt ctttaccgag ctctgctttg ccttcgtgga atcctgaagg gtaagaagct
    27001 cctgatgctc cgtcttaaga gcttcctcct ttaaagccac ctcctgcctg gtttttctgt
    27061 actcctccag cattttcctg tcataggagg taatctggct ggcatactct gcccggttta
    27121 aaagctctgc aatgctctct gactgcatca tggactccaa caggccagtc tgaccatttt
    27181 catacatata ctggatccgc agcttcatgt cctcatactg gcggttctcc gtaattttgg
    27241 ctgtttccag ctccactttc gcttcctcta tctgctcttc cttctgggaa atatctccct
    27301 ccagctttgt aagctcccct gccagggcgg acagatttcg atccagctcc tttacatagg
    27361 ccgctgtatc tgacttcttt ccctccagct cctttaaagt agactctacc tttttctttt
    27421 cctcctccat ggaagaaacc tttttcttcg catcctctat agccttactg gtggcatagg
    27481 aggaaaatac cgttcccaag gtcaaggcgg ccgctgctgc caaaaccgct atcttctttt
    27541 ttctcatatg ccctcgttta tcctgcctaa actctcagat ggcgctggat cgtcacaaag
    27601 cttactaaaa gtccgattcc gacgcctaac attaaagctc cccctgtcat ataggggaat
    27661 atggttccca gcgcaagggg ctcaaccaga cctgtgatca tggaataatt ttcttccaga
    27721 tacaacattc ctttttcata gagaacccat atgcccccaa gaggcacaag agctcccaga
    27781 aatcccagaa gcaggccttc taccagaaag ggcgcccgta tcataaagtt agtggctcct
    27841 atatagcgca tgatctcatt ttcccgcctg cggaatgccg ctgccacaga aatagtattg
    27901 ctgatcaaaa aaacagctac cgcaagaagc acgccgatga tcagagccga aatcaatccg
    27961 atcatctttc caacgcttgt aaatcctgct gccagggtac tggaataccg cacccggcgc
    28021 accccatcca tggcctccag gcggtctgca accgactcct gatcggaaat atcgtttaaa
    28081 aaaatctcaa aggaagcaga atttgccaga ggattatcgt tttcaaaccc ttcggccaaa
    28141 tcttcggctc cctcaaagta ctgagcctta aatgtatccc aggcttcctc ggctgaggtg
    28201 tactctgcct gacgcacctc tttccagcct tttatttctt ctcccgctgc cagaatttca
    28261 tcctctgtca tcccctcatc gaaaaagaca gagattccca ccgtttcctg ggctgtcctg
    28321 gcgccatgga tcacattggc tgccagtgag aaaaaaaggc agaataaaaa aatacatgca
    28381 gaaatcgtcg ctatggatgc cagcgaaaac aaaatatttc tgcatatatt agcaatgccc
    28441 tgtttcaggc aataccagaa tgtactcatt tttaatcatt atgcctttcc ggcagacccg
    28501 tccggacgct taagcgcctc attttcttat ctggatccgc cttcatcctg aatcactgcc
    28561 ccgcgttcca tggtaatgac ccgttttttc atctgctgca ccatctcatg gctgtgtgtc
    28621 accacgatca tcgtggttcc acgctgattg atctcctcca gcagatgcat aatttcatgg
    28681 gcgttaaagc tgtctaaatt gcctgtaggc tcatctgcca gaagaacctc cggccggttg
    28741 atcaatgccc ttgcaatggc cacgcgctgc tgctctcctc cggaaagctg agccggatat
    28801 gccttgtatt tggcagaaag ccccacaaga cgcagcatct cgggcacggt gtcacggatc
    28861 acccttcccg gcacgccgat gactctctga gcaaatgcaa cgttttcaaa gacagtaagg
    28921 tctttcagga gacggaaatc ctgaaagacc acacctaatt ttctccggta tttcggaaca
    28981 tatcttcggg gcatcttccc cagatccata tcattgacca caatccgccc ggaagtaggc
    29041 tcaagctcct ttaaaaggag ctttaaaagc gtactctttc cggagccgct tcgtcctacg
    29101 ataaaggcaa attctccgtc atctatggtc aggctcagat ccttcacagc ccggtttccc
    29161 tcgctgtaga ccttgttcac atgactgagt tctatcattt ccccgacctc attttaataa
    29221 tccaggcttt ccatatactt catgtatttg gctaccataa gcgcgatctt aaaggtaatg
    29281 gcatcctcaa atacgcgaag atccagtccg gtgctcttct gcagcttatc cagacggtaa
    29341 accagggtat ttctgtggat atacagctgt ctggaagtct cggatacgtt caggctgttc
    29401 tcaaagaact tgttgatcgt agccagggtc tcctcgtcaa actcatccgg tgacttgccg
    29461 tcaaatacct cgcggataaa catcttgcac agcgggatgg gaagctgata gatcagacgg
    29521 ccgataccca gattgctgta agcgattaca tttttgtttg catagaagat gcggcctaca
    29581 tcaagggcca tcttggcctc cttgtaggaa cgtgacactt ccttaatatc attgatcacc
    29641 gtaccatagg atacgcgaac ctttgtcata gcctctgtat tcatcatatc caaaattgtg
    29701 ttggcagtct tgtccagctc tccgtaagac tctccctgac gaacctcttt tacaaggata
    29761 atattcttct catccactgc tgtaatgaag tcctttgtct tggatgcaaa aaggctgcgc
    29821 acggtctcaa gagcatttac atccttttca ttgtgtgttt caatgataaa taccacgcgc
    29881 ttcacatctg tttcaatatg aagcttcttg gcacggttat agatatctac taacagcaga
    29941 ttgtcaagga gcaggttctt gataaagttg tccttatcaa agcgctcctt ataggccacc
    30001 agaaggttct ggatctggaa tgcagccagc tttcctacca tatatacatc atcgctggct
    30061 ccctttgcaa gcaggatata ctcaagctga tgctcgtcaa ataccttaaa gaactggtat
    30121 ccctggatca cctggctgtc tgccggagaa tccacaaata ccaggatcga actttctgac
    30181 tcttccgcgt ctgtaaaggt ggataccagc acctttcctt ctgtgtcgca aatacctaaa
    30241 tcaattcttg tgattgcttt taatccatcc agtgtagtct ggagtatctg atttgaaatc
    30301 attccatact ctcctttttg acgtatttca aaagtttcca ctttctctat tttcatattt
    30361 taatgcaaaa ttacaaaaaa agcaagagag ttgtcacctc tcct
//
LOCUS       DBMDUH010000009        30230 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_1136_length_30230_cov_5.126357, whole genome shotgun sequence.
ACCESSION   DBMDUH010000009 DBMDUH010000000
VERSION     DBMDUH010000009.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 30230)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 30230)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30230
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1136_length_30230_cov_5.126357"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="ACZPCG_01840"
     CDS             complement(<1..866)
                     /locus_tag="ACZPCG_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HIR1283846.1"
                     /translation="MTYSAVNTIWVLFGAAMVFFMQAGFSMCEAGFTRAKNTGNILMK
                     NLMDFCIGTPAFWLVGFGLMFGAGSGVIGTFDPLIGGDYSHILPAGVPLWAFVIFQTV
                     FCATSATIVSGAMAERTKFSAYCIYSAAISLLIYPVSGHWIWGGGWIAQMGFHDFAGS
                     TAVHMVGGVCALIGAKILGPRIGKYDKNGKPQAILGHNLSFAALGVFILWFCWFGFNG
                     ASTVGMDSDALMDTAGRVFFNTNMAAAVACCTTLLFTWIRYKKPDVSMTYNAALAGLV
                     GITAGCDAVSPVG"
     gene            complement(916..3012)
                     /locus_tag="ACZPCG_01845"
     CDS             complement(916..3012)
                     /locus_tag="ACZPCG_01845"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016439164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III family protein"
                     /protein_id="HIR1283847.1"
                     /translation="MKKVSDYFGCKVFDDRVMKARLSSKVYQSLRKTIDEGAKLDISV
                     ANAVAAAMKDWAVENGATHYTHWFQPLTGVTAEKHDSFISPSPDGGVIMEFSGKELIK
                     GEPDASSFPSGGLRATFEARGYTAWDPTSYAFIKGKSLCIPTAFCSYGGEALDKKTPL
                     LRSMEALNRQAMRILHLFGNEDVKCVRTSVGPEQEYFLITEEMYEQRKDIRFTGRTLF
                     GAKPPKGQEMDDHYFGVIKPRVAAYMDELNEELWKLGILAKTEHNEVAPAQHELAPIY
                     TTTNIATDHNQLTMEIMQKVAAKHGLVCLLHEKPFAGVNGSGKHNNWSLATDKGVNLL
                     SPGETPYENAQFLLFLCAVIKAVDDYQDLLRLSVATAGNDHRLGANEAPPAVVSIFLG
                     DELNGVLEAIENDTPYAGTEKTQMKLGVNVLPKFNRDTTDRNRTSPFAFTGNKFEFRM
                     LGSSNSIACANIMLNSAVAESLKIYADRLEKAEDFESALHEMIRKTIKDHKHIIFNGN
                     GYDDTWIKEATEKRGLLNYRTTPDCMPHLLDEKNVRMLTSHKVFSEAELKSRCEIMLD
                     NYCKTVLIEANTMIDMAKTEIAPAVSEYVLELAKTAAAKKAAESSISCNYETGLLKKL
                     AILEDQIMIKAEELEDAVLKVQDITDITEESCMIRDSVLSKMGELRIACDSAETMTAK
                     KFWPFPTYGDLLFSVR"
     gene            3301..4395
                     /locus_tag="ACZPCG_01850"
     CDS             3301..4395
                     /locus_tag="ACZPCG_01850"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016439166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HIR1283848.1"
                     /translation="MKIEGQVRIPSGCAISAVISKDGNKMSGESITNSMKPMHDRSNG
                     LGGGFAGYGIYPEYKEFYALHMFFDTRDTRKKCEAFLKEQFEIVQSEVIPTRKLPAIT
                     DEPIIWRYFVAPLKSVLASMQLDEKEFVAQTVMKINAEMSGAYVFSSGKNMGTFKAVG
                     FPEDVGVFYKLEEYEGYSWTAHGRYPTNTPGWWGGAHPFTLLDYSIVHNGEISSYDAN
                     RRFIEMFGYKCTLQTDTEVITYIMDYLLRVQGLTLGEAASVIAAPFWSTIASKTDLED
                     QKKHTYLRTMFSSLLITGPFSIVLGFNGGLMALNDRLKLRSMVIGEKDDKVFIASEEA
                     AIRTMEPNAENIWSPAGGEPVIVKVKEGAF"
     gene            4395..5900
                     /locus_tag="ACZPCG_01855"
     CDS             4395..5900
                     /locus_tag="ACZPCG_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HIR1283849.1"
                     /translation="MGIEFIYPEFEVIRNEDRCTVCRACERQCANEVHSYDEELGVMK
                     CDESKCVNCQRCVSFCPTRALKIVKTDCSLRENSNWQNNTIKEIYKQANSGGVLLSSM
                     GNPKPFPVYWDKILINASQVTNPSIDPLREPMETRTYLGKKPQRVSRNAEGKLDCQLP
                     PQLDLSMPVMFSAMSYGSISYNAHKSLALAATELGILYNTGEGGLHEDFYCYGENTIV
                     QVASGRFGVHEKYLNAGAAIEIKMGQGAKPGIGGHLPGAKIVGDVSRTRMIPEGSDAI
                     SPAPHHDIYSIEDLRQLVFSLKEASEYKKPVIVKVAAVHNIAAIASGIARSGADIIAI
                     DGFRGGTGAAPTRIRDNVGIPIELALAAVDQRLRDEGIRNDVSLVVGGSIRSASDVIK
                     AVALGADACYVGTAALLALGCHLCQSCQTGKCNWGIATQRPELVKRLNPETGKKRLVN
                     LMTAWNHEIKELMGGMGINSIEVLRGNRLMLRGIGLTAKELEILGISHAGE"
     gene            5948..6670
                     /locus_tag="ACZPCG_01860"
     CDS             5948..6670
                     /locus_tag="ACZPCG_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004220485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GltB/FmdC/FwdC-like GXGXG domain-containing
                     protein"
                     /protein_id="HIR1283850.1"
                     /translation="MLIIDAANSDYKAVNEKLRNTDEDCTIEGCCGQRFIAAGMSGKN
                     VTIHGIPGNALGAYLNDSHITVNGNAQDAVGDTMNEGKILIYGNIGDAAGYAMRGGKI
                     YVQGNAGYRAGIHMKAYKEKIPVMIIGGCAGSFLGEYQAGGIIIVLGLHKNGKSIVGN
                     FPCTGMHGGKMFLRGDCGNIRFSEQVTAKTAGDEDLEEIREYLSEYCADFGYSLDGIL
                     SEPFTVITPDSKNPYKQMYVAN"
     gene            6691..7902
                     /locus_tag="ACZPCG_01865"
     CDS             6691..7902
                     /locus_tag="ACZPCG_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004220488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HIR1283851.1"
                     /translation="MKYSKEEVLQYMQEEDVKFIRLAFCDVFGKQKNISIMPQELCRA
                     FEHGIAIDASAVAGFGDESHSDLFLHPDPDTLMPLPWRPEHGRVVRMFCSITYPDGTI
                     FECDTRSLLKKAVHEAEQAGYQFFFGAEQEFYLFHLDENGNPTKIPYDHASYMDMAPE
                     DKGENIRREVCLTLEQMGIFPESSHHEEGPGQNEIDFRYSDPLAAADNSMTFQTVVKT
                     IAGLNGIFADFSPKPLENEAGNGFHINMSVKSSDGTDHMDSMIAGILDKISDMTVFLN
                     PTADSYRRFGCNKAPKYISWSSENRSQLIRVPAAIGRYRRVELRSPDPSANTYLAFAL
                     MIYAVLEGVQNNLALPPAADINLYRAEASVLSGFGQLPKNFEAACDAAAHSDFILKYI
                     PKTILDIYCGQ"
     gene            7925..8509
                     /locus_tag="ACZPCG_01870"
     CDS             7925..8509
                     /locus_tag="ACZPCG_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing response regulator"
                     /protein_id="HIR1283852.1"
                     /translation="MSLKERTYSILIVSATDVLTSAFAGLLPETRYSPVHAVSSINAA
                     RRTLTEKTYDFIIINAPLHDDMGVRLAIDISSSKQSVVLLLVKSDIHNEIHDQAAEYG
                     VFTLSKPISKATMTYAFSWLESARERLRQFEKKSLSIEEKMAEIRLVNKAKWILISQL
                     SMSEPESHRYIEKQAMDRCVTRRSIAEEIIRTYS"
     gene            complement(8513..9790)
                     /locus_tag="ACZPCG_01875"
     CDS             complement(8513..9790)
                     /locus_tag="ACZPCG_01875"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HIR1283853.1"
                     /translation="MVRAIVGANWGDEGKGKITDMLAKESDIIIRFQGGSNAGHTIIN
                     EYGKFALHLLPSGVFYQHTTSIIGNGVALNIPYLIKEIQSLVDRGVPKPHILVSDRAQ
                     ILMPYHVLFDTYEEARLAGKSFGSTKSGIAPFYSDKYAKIGFQVSELFDEELLREKCQ
                     RVCEIKNVLLEHLYHKPLLNADELFDELISYREMIRPYVCDVSSYLYHAIKEGKNILL
                     EGQLGSLKDPDHGIYPMVTSSSTLAAYGAIGAGIAPYEIKNITTVVKAYSSAVGAGAF
                     VSEIFGEEAEELRHRGGDGGEYGATTGRPRRVGWFDAVATRYGCRIQGTTEAAFTVLD
                     VLGYLDEIPVCIGYEIDGEVTREFPTTAKLEKAKPVFTRLPGWKCDIRGIKNYEELPE
                     NCRRYIEFIEKEIETPITMVSNGPGREDIIFRK"
     gene            complement(9939..10838)
                     /locus_tag="ACZPCG_01880"
     CDS             complement(9939..10838)
                     /locus_tag="ACZPCG_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006779635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1283854.1"
                     /translation="MNINLEYYKVFYYVCTAGSLTAAAQKLCISQPAVSQAVRQLEKE
                     AGTRLFLRTSKGIRLTREGELLFRYVKTGVEQLLEGGRMLERMLDMDMGEVRIGASDM
                     TLQFFLLPFLEQFHKEYPRIKVTVTNAPTPETICSLEEGKIDFGVVTTPFSCKSTIHS
                     MMVKPIGTVFIAGDSFGELKGKKLDYSILGSMPCIFLEKNTSTRAFMDAFLSAKGMEL
                     TPEFELATSDMIVQFAKRNMGVGCVMDGFAKDAIAKGEVFELDFKEKMPLRHICVVTG
                     ENSLISLAGRKLLNLFDEMAESS"
     gene            complement(10855..12291)
                     /gene="purB"
                     /locus_tag="ACZPCG_01885"
     CDS             complement(10855..12291)
                     /gene="purB"
                     /locus_tag="ACZPCG_01885"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HIR1283855.1"
                     /translation="MSNHDTYVSPLSERYASREMQYIFSPDMKFKTWRRLWIALAETE
                     KELGLNITQEQIDELKAHADEINYDVAKAREKEVRHDVMSHVYAYGTQCPKAKGIIHL
                     GATSCYVGDNTDVIIMTEALKLVRRKLLNVLAELAAFADKYKNLPTLAFTHFQPAQPT
                     TVGKRAALWMMELKLDLDDLDYLIGSMRLLGSKGTTGTQASFLELFDGDHERCRRLDQ
                     RIAEKMGFEGCYPVSGQTYSRKVDSRVLSVLAGIAQSAHKFSNDIRLLQHLKEVEEPF
                     EKNQIGSSAMAYKRNPMRSERIASLANYVMSDMMNPMLVASTQWFERTLDDSANKRLS
                     IPEGFLAVDGILDLYLNVVDGLVVYPKVIEKHLMAELPFMATENIMMDAVKAGGDRQE
                     LHERIRQLSMEAGKNVKEQGGDNNLLELIAADPAFGLSLEDLKKAMEPSRYVGRAPRQ
                     VEEFLEEVIRPVLEANKEALGMTAEINV"
     gene            complement(12425..12667)
                     /locus_tag="ACZPCG_01890"
     CDS             complement(12425..12667)
                     /locus_tag="ACZPCG_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1283856.1"
                     /translation="MLIFVCICLETASAKDWQSGNRTAEIRIDSLPAFIEIHPFYEEE
                     FRREKENASRTEQGISLFLKEGRIVFFRIHEEACDD"
     gene            complement(12954..13286)
                     /locus_tag="ACZPCG_01895"
     CDS             complement(12954..13286)
                     /locus_tag="ACZPCG_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1283857.1"
                     /translation="MMKKIRPVMMVLLIAVLASSLTACGSRDSKQPTASSGTPGTSAS
                     YSTETDQGAAANETENTDATSRSETDTTGEGVMDGIADDVRKGVEDIKRDIMDTTESE
                     STAETQNR"
     gene            complement(13405..14163)
                     /gene="dapB"
                     /locus_tag="ACZPCG_01900"
     CDS             complement(13405..14163)
                     /gene="dapB"
                     /locus_tag="ACZPCG_01900"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HIR1283858.1"
                     /translation="MINLIMHGCNGAMGRVISAMAQEMDGIRIAAGVDLNTEKNYDYP
                     VYASLGECTEAADAVVDFASAKAVDELLDYCLEKELPLVLCTTGLTEEQLKRAEDASK
                     RIPVLRSANMSLGVNLLLKLIQDAAKVLGNAGFDMEIVEKHHNQKKDAPSGTALAMAE
                     AVNEAMGGLYHFVTDRSSIHEKRDGKEIGISSVRGGSIVGEHDMIYAGKDEVITISHT
                     AYSKAIFAKGALEAAKFLAGKQPGLYTMTDVIGL"
     gene            complement(14229..15116)
                     /gene="dapA"
                     /locus_tag="ACZPCG_01905"
     CDS             complement(14229..15116)
                     /gene="dapA"
                     /locus_tag="ACZPCG_01905"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HIR1283859.1"
                     /translation="MAVFEGAGVALITPFKENGEVNYDKLEEIVEEQIAGGTDAIIAC
                     GTTGEASTMTHEEHLDVIGFICDVTKKRIPVVAGTGSNCTQTAIYLSKEAEERGADGI
                     LVVSPYYNKATQKGLKAHFTAVADAVKIPMILYNIPGRTGVNIQPETIASLCREVDNI
                     VGVKEATGNFSGIAQLMNLTDGNCDVYSGNDDQIVPLLSLGGKGVISVLSNVAPSQAH
                     EICASYFAGDVKKSARMQLEAIPLINALFCEVNPIPVKAAMNLMGKEVGPLRMPLTEM
                     EPEHQEILKKAMKEYGILA"
     regulatory      complement(15285..15458)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            complement(15504..16001)
                     /locus_tag="ACZPCG_01910"
     CDS             complement(15504..16001)
                     /locus_tag="ACZPCG_01910"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HIR1283860.1"
                     /translation="MMKDSTIQVICGPGKGKTASALGRGISALIHGKNVIMVQFLKGS
                     MDSDNMEVLKRLEPEFKLFRFEKSPVVFDQLSEEEKEEARINIRNGLNFSKKVLVTGE
                     CDILILDEILGILDEGILTLEELCALIGQARQSEAELIMTGTVYPAALDEWVDEVTKL
                     QTRFE"
     gene            complement(16137..16775)
                     /locus_tag="ACZPCG_01915"
     CDS             complement(16137..16775)
                     /locus_tag="ACZPCG_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIR1283861.1"
                     /translation="MAEKMIENNRVSVIGEIVSEFSFSHEVFGEGFYMVDLSVNRLSE
                     QADVIPLMVSERLMDVHKDLRGSTIEAVGQFRSYNRHEGVKNRLMLSVFAREIRFLDE
                     FTDYTRTNQIFLDGYICKPPIYRKTPLGREIADLLLAVNRPYGKSDYIPCISWGRNAR
                     FASGFTVGTRVRIWGRVQSREYTKKLSETECEKRVAYEVSISKLECGEESDQ"
     gene            complement(17026..18855)
                     /gene="typA"
                     /locus_tag="ACZPCG_01920"
     CDS             complement(17026..18855)
                     /gene="typA"
                     /locus_tag="ACZPCG_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007711892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HIR1283862.1"
                     /translation="MKMKREDVRNIAIIAHVDHGKTTLVDQLLKQSGVFRANQEVQER
                     VMDSNDIERERGITILSKNTAVFYKDTKINIIDTPGHADFGGEVERVLKMVNGVVLVV
                     DAYEGPMPQTKFVLQKALELNLSVIVCINKIDRPEARPEEVIDETLELMMDLDASDEQ
                     LDCPFIYASARAGFAKFNIDDPEVDMGPLFETIVNHIPAPEGDPDADTQVLISTIDYN
                     EFVGRIGIGKVENGGLKVNQECVLVNHHDPDKFKKIKIGKLYEFDGLKRVEVQEAKIG
                     SIVAISGIADIHIGDTICSPQNPEAIPFQKISEPTIAMYFMVNDSPLAGQEGKYITSR
                     HLRDRLFRELNTDVSLRVEETDSADCFKVSGRGELHLSVLIENMRREGYEFAVSKAEV
                     LYHYDERNRRLEPMELAYIDVPEEYTGVVIQKLTARKGALQGMSQAAGGYSRLEFSIP
                     SRGLIGYRGDFMTDTKGNGILNTIFDGYAEYKGDLSYRPTGSLIAFEAGEAITYGLFN
                     AQERGTLFIGPGVKVYSGMVVGQSPKAEDIEINVCKTKKLTNTRSSSADEALKLTPPK
                     IMSLEQSLDFIDTDELLEVTPESLRIRKKILDPLLRKRASFKK"
     gene            complement(18961..19611)
                     /locus_tag="ACZPCG_01925"
     CDS             complement(18961..19611)
                     /locus_tag="ACZPCG_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HIR1283863.1"
                     /translation="MVESYKIVYEGADGELVEKKSRFIAQIRPVASEEEAFAFIEEVR
                     KKYWDARHHCYAYIIGERAQTARCSDDGEPSQTAGKPMMDVLAGEGLHDVCAVVTRYF
                     GGTLLGTGGLVRAYSGAVKEALLNCSVVEKKLGVQLEVTADYNSVGKIQYIAAQEGID
                     TLESRYSDKAEFDFLVPVAVKDSFQAKITEGTNGRARIRTGKELYYGMNRGELIVF"
     gene            19818..22520
                     /locus_tag="ACZPCG_01930"
     CDS             19818..22520
                     /locus_tag="ACZPCG_01930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR1283864.1"
                     /translation="MNYGTHESERRLRALQSKSAKYTSKLFFNIFKAFFVLFLFLAIA
                     GTAIGFGMVKGIIDSAPSVDLMNIQPDRFATTVYDIGGNLTDTIVTSGSNRESAAYEE
                     LPQDLIDAFVAIEDSRFWSHNGIDIRSIARAVNGLISDDYAGGGSTITQQLIKNNIFA
                     GGMETSWGARIERKLQEQYLAVHLEKNSGMSKEETKKVIITNYLNTINLGYNTLGVKV
                     AARRYFNKEVSDLTLSECAVLASITKNPSRLNPISGAEANAERRSIVLQYMYDQDYID
                     KQELEEALADNVYERIQNVNTAAQETNSHFSYFTDELVEQVIAALMGELGYSESQARN
                     LLYAGGLKIYTTQDPKLQAIVDEEINNPENYTAAKYSVEYRLSITHTDGTTEHFSEEN
                     LKSYHKSVLGDTGYDGLYPSKEAVQEDIERYKAWLIKDGDTIIGERQNLILQPQASFV
                     LMDQRTGEVRALSGGRGEKTASLTLNRASNVYRQPGSAFKVITAFAPALDTCGATLGT
                     VYYDAPYTIGTKTFRNWWGSGKGFVGYSNIREGIIYSMNIVAVRALMETVTPQLGMEY
                     AEKMGITSMTKNDLGAAQALGGLTKGVSNLELTAAYASIANGGVYTKPRFFTKILDHD
                     GNVLINNEPETRQVLKDSTAFLLTDAMADSMKANRKFARSGVSINSTSTRAALSGMSA
                     AGKSGTTTGNNDIWFVGFTPYYTAGVWGGCDSNQKLSAGNGGTSFHKDIWRNIMNRVH
                     EGLSDPGFPVPDSVETAEICRKSGKRAVPGVCDHDPRGSAVYTEYFAKGTAPSEVCDK
                     HVAVTVCAQSGLRPTPYCTEKTTKVCMVLPEGEENLATDDSVFSIPGTCTIHSGNSTI
                     IAPSFGSDETGGSGENVTISPIGPGYEPQTRPQWENSGPGVRQN"
     gene            complement(22598..23233)
                     /locus_tag="ACZPCG_01935"
     CDS             complement(22598..23233)
                     /locus_tag="ACZPCG_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1283865.1"
                     /translation="MKDKRSNRYKTAAWILLIAGIVSFSVINIFSLFFHYSNRIHNEI
                     LNRNMEQISLLSDYIVKIIRSEMRHCTEILNIGEENFSVPEKMTSAEIVKQLQEIRSR
                     AGFAQIGIMDQEGNTIDDTGNRWQIDDSELLAAMEKGRAYVSDVFTSGRQDTSQIMIM
                     VPLWELQKQKRGWVLEIFMKERTGRFIRRRNRGGTDIILQSKTAEDPIGPA"
     gene            complement(23369..23722)
                     /locus_tag="ACZPCG_01940"
     CDS             complement(23369..23722)
                     /locus_tag="ACZPCG_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVA/SpoVAEb family sporulation membrane
                     protein"
                     /protein_id="HIR1283866.1"
                     /translation="MDYIKAFVTGGIICALVQILMEKTKLMPGRIMVLLVVSGAILGA
                     VGIYEPFLSWAGAGASIPLLGFGNTLFKGVKTALAEEGVLGLFKGGLTAASAGITGAL
                     IFGYSASVLFKPKMK"
     gene            complement(23771..24790)
                     /locus_tag="ACZPCG_01945"
     CDS             complement(23771..24790)
                     /locus_tag="ACZPCG_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003523824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HIR1283867.1"
                     /translation="MEQKKGKATVAFARPPVICGAASIVGKKESEGPLGPFFDRHIMD
                     DGKGMENWEQAESSMQKAAADLSITKAGLKRDDIRYLFAGDLLGQLIATSFGTVDLEI
                     PLFGLYGACSTMGEAMSLGAMMVNAGYADHVLSMASSHFATAEKQFRFPLAYGNQRPM
                     SSTWTVTGCGAVVIGKEGQPGLAKITAFTAGKAIDIGARDSMNMGAAMAPAAFHTIEQ
                     NLEDLGTDETWYDRIITGDLGEVGTQILLDLMEKKGRSLKERHMDCGVEIYSKETQDT
                     HAGGSGCGCSAVTLCAAILPKIREGIWKRVLFVPTGALLSTVSYNEGQTIPGIAHGVV
                     IEHLE"
     gene            24980..25639
                     /locus_tag="ACZPCG_01950"
     CDS             24980..25639
                     /locus_tag="ACZPCG_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR1283868.1"
                     /translation="MDLSNNFSVWNHLTPAQQELISTHLICRDVKKGTVLYNGSSDCL
                     GLVLIKKGQLRVYMISEEGREVTLYRLLERDICLFSASCIMKNIQFDITIEAEKDSNI
                     CIIPAVIYKRLMEESAAVSNYTNEIMAARFSEVMWLIEQIMWKTFDRRLASFLLEEMA
                     LEGSSGLKITHETIAGHLGTAREVVTRMLRYFQEEGMVKLSRGMINITDEKRLRKLAE
                     S"
     gene            complement(25693..26268)
                     /locus_tag="ACZPCG_01955"
     CDS             complement(25693..26268)
                     /locus_tag="ACZPCG_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR1283869.1"
                     /translation="MKKHWYDYMWILSLTYLILGFFNIMFAWLGLLCFFIPLIIALVQ
                     GNKGYCNRYCGRGQLFGLLGGRLGLSRKKDIPGWMKSRAFRYGFLVFFLIMFCQMLWN
                     TVLVFTGASDLKQVVTLLWIFKLPWHWAYHGTLFHPGTAQFAFGFYSVMLTSTILGLI
                     TMVLFKPRSWCVYCPMGTMTQLICKGKNGKK"
     gene            complement(26268..26477)
                     /locus_tag="ACZPCG_01960"
     CDS             complement(26268..26477)
                     /locus_tag="ACZPCG_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR1283870.1"
                     /translation="MATGVRKKRKASVDSRLCVACGCCVKVCPLGAIYVADGMWAQVN
                     LEKCVGCGKCAKKCPASVIEIREVG"
     gene            complement(26644..26985)
                     /locus_tag="ACZPCG_01965"
     CDS             complement(26644..26985)
                     /locus_tag="ACZPCG_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HIR1283871.1"
                     /translation="MNIQIFGTKKSFDSKKAERYFKERGIKYQFIDMKEKGLSKGEFN
                     AVCRAVGGYEKLIDPDCRDKDLLALIRYIAEEDRAEKILENQKILKVPIVRNGKQAAI
                     GYEPDIWKEWK"
     gene            complement(27004..28965)
                     /locus_tag="ACZPCG_01970"
     CDS             complement(27004..28965)
                     /locus_tag="ACZPCG_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HIR1283872.1"
                     /translation="MADLQTRYLERLAELYPTVAAASTEVINLEAILNLPKGTEHFLT
                     DIHGEAEAFSHVLKNGSGAVRRKVNDVFGNTLCREDKRSLATLIYYPREKMAQILEHT
                     ANKEDWYKITLYRLIEVSKRSGSKYTRSKVRKALPPEFAYVIEELMTEKADIRDKESY
                     YTAIIATIIRVGRAEEFIRALCELIQRLVVDHLHILGDIYDRGPGPHLIMDKLMQYHS
                     LDIQWGNHDVLWMGAAAGQRGCIANVIRICARYGNLDILEDGYGINLLPLATFALETY
                     GDDPCICFALKGSHPCSPGEKELNLRMHKAISIIQFKVEGQLIKEYPEFGLERRLLLH
                     RINYENGTIVLDGRRLPMKDMHFPTVDHADPYRLSPEEESIMERLEKAFLNCEKLQQH
                     MRFLLAKGSLYKVYNQNLLYHGCVPLCEDGSLKETSVYGKRYKGRGLYDVLESYIRRG
                     FMAEDETERKRGRDIMWYIWLGENSPLFGKDKMATFERYFLEDEESHKEKKNPYYRML
                     ENEKTVDMILGEFGLGGEESHIINGHVPVKSKNGENPVKCGGKVLVIDGGFSRAYQKE
                     TGIAGYTLIYNSYGLILAAHEPFESRAAAIEQESDIYSEDIIVKRTASRKLVGDTDTG
                     QQLKEKIADLEQLLKAYRDGRIAERFGRR"
     gene            complement(28991..>30230)
                     /locus_tag="ACZPCG_01975"
     CDS             complement(28991..>30230)
                     /locus_tag="ACZPCG_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007719093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR1283873.1"
                     /translation="NQRFRLYINWGLTALAVIACSVFLAFSLMKVQTIFGVIGEIIQI
                     LMPIIYGGVLAYLMLPVYNSVYFFVCRNTKKLFTTEKVRSRAGKTAATIASLLFLLIL
                     VCGLVWMIIPQIMESILGFQESLAEYINNLTLWLSQLLEDNPELEEIVMPHFNEAAME
                     FQHWMTTDLVPSIYLILDHVSAGVINAVTVVKNILIGLIVMIYLLNLKWTLCAQFKKI
                     IYGLFSVRMANRLINEIRFAHHVFGGFITGKLLDSLIIGIMCFFGMRFLKMPYVLLIS
                     VIIGFTNVIPFFGPFIGAIPSAFLILLVSPLKCLYFLIFILVLQQFDGNILGPKILGD
                     STGLPSFWVLFSILLFGGLFGFVGMIIAVPVFAVFYRLISEYICGMLRRKKLSARTDD
                     YRELDHIDPIEGTFIEHTEK"
BASE COUNT         7752 a         7842 c         6483 g         8153 t
ORIGIN      
        1 ccaaccggac ttaccgcgtc acatccggcc gtaattccga caagacctgc cagcgccgcg
       61 ttatacgtca tggaaacatc cggcttttta taacggatcc atgtgaacag cagggtcgta
      121 cagcaggcta cggctgccgc catatttgta ttgaaaaata cccgtcccgc cgtatccatc
      181 agcgcgtcac tgtccatgcc taccgtagac gcgccgttaa atccgaacca gcagaaccag
      241 agaataaaca ctccaagggc tgcaaaggaa agattatgtc ccagaattgc ctgcggtttc
      301 ccgtttttat catattttcc aatacgggga ccaagaattt tggcgccgat caaagcgcag
      361 acaccgccta ccatatgtac tgccgtagaa cctgcaaaat catgaaagcc catctgcgca
      421 atccagccgc ctccccagat ccagtgaccg gaaaccggat agatcagcaa tgagatcgca
      481 gcggaataaa tgcagtaagc gctgaatttt gttctctctg ccattgctcc ggaaacgatt
      541 gtggctgatg tggcgcagaa tacagtttga aatatcacaa acgcccaaag ggggacgccg
      601 gcaggcagga tgtggctgta atctcctccg atcaggggat caaaggttcc tatgacgccg
      661 gaacctgcgc cgaacataag tccaaagcct accagccaga aggcaggagt gccaatgcaa
      721 aagtccatca gattcttcat caggatattt ccggtatttt ttgctctggt aaaacctgct
      781 tcacacatag aaaaacctgc ctgcatgaaa aataccattg ctgctccaaa cagtacccag
      841 attgtgttaa ccgcactgta cgtcataaac attccttctt tctgttgttg tatgatccag
      901 agtttccgcc gaaacttatc ttacgctgaa taataaatct ccgtaagtcg ggaagggcca
      961 gaatttcttt gcggtcatgg tttctgcact gtcgcaggcg attcgcagct ctcccatttt
     1021 acttaaaacg ctgtcccgga tcatgcagga ttcctcagtg atatccgtaa tatcctgaac
     1081 cttaagcact gcatcctcca attcctctgc cttgatcatg atctgatcct ccagaattgc
     1141 cagcttcttc agcagtccgg tttcataatt gcaggaaatc gaactctctg ccgctttctt
     1201 tgcagccgct gtttttgcaa gctccagcac gtattcgctt acagccgggg caatttcggt
     1261 ttttgccata tcgatcatgg tatttgcctc gatcagaacg gtcttacaat agttgtccag
     1321 catgatctcg catctggatt ttaattctgc ctctgaaaat actttatggg aggtaagcat
     1381 ccgtacattt ttttcatcca gaagatgagg catgcagtcc ggcgtggtcc ggtagttgag
     1441 aagtcccctc ttctccgtag cttccttaat ccatgtatca tcatatccat ttccattaaa
     1501 gataatatgc ttatggtctt tgatggtctt tcggatcatt tcatgtaaag cgctttcaaa
     1561 atcctctgct ttttccagtc ggtctgcata gattttcagg ctttcggcta ctgcgctgtt
     1621 taacatgata ttggcgcagg caatactgtt ggatgagccc agcatacgga actcgaactt
     1681 atttccggta aatgcaaagg gtgaagtacg gttgcggtcc gtggtatccc tgttaaattt
     1741 gggaagcaca tttaccccca gcttcatctg ggttttttct gtgcccgcat atggggtatc
     1801 gttctctatt gcctccagaa ccccgtttaa ttcatctcca aggaagatgg ataccactgc
     1861 gggaggcgct tcatttgctc caagccggtg atcatttcct gccgttgcca cggaaaggcg
     1921 gagcagatcc tgataatcat ctaccgcttt gatcactgca cacaggaata ataaaaactg
     1981 tgcgttctca tagggtgttt cacccggcga aagcaggttt acgcccttat ccgttgccag
     2041 ggaccagttg ttgtgcttac cgcttccatt gactcctgca aaaggctttt cgtggagaag
     2101 gcataccaga ccatgctttg ccgctacctt ctgcattatt tccatagtaa gctgattgtg
     2161 atcggttgca atattggttg tggtatagat gggggcaagc tcgtgctgtg ccggagctac
     2221 ttcgttatgc tctgtttttg caagaatccc cagcttccag agttcttcat tcagctcatc
     2281 catataggcg gctactctcg gcttaattac gccaaaataa tgatcatcca tctcctgccc
     2341 ctttggcggc ttcgcgccga aaagggtacg ccctgtaaaa cggatatcct ttctctgctc
     2401 atacatttcc tctgtaataa ggaaatactc ctgctccgga ccgaccgagg tgcggacgca
     2461 tttcacatcc tcatttccaa aaagatggag gatacgcata gcctgtctgt ttaaggcttc
     2521 catggagcgg agaaggggag tttttttatc aagcgcctct cccccataag aacagaatgc
     2581 agtggggatg cacagtgact tccctttaat aaatgcgtat gaggtaggat cccatgccgt
     2641 atatcccctt gcctcaaagg tagccctaag gccgccggac ggaaaagaag atgcgtcagg
     2701 ttctcccttg atcagttctt ttcctgaaaa ttccataatc acaccgccat ccggcgacgg
     2761 ggaaataaaa ctgtcatgct tttctgccgt tacgccggta aggggctgaa accagtgtgt
     2821 ataatgtgtc gctccatttt caaccgccca atctttcatg gctgccgcaa cggcattcgc
     2881 cacactgata tcaagctttg cgccttcatc aatggttttt ctcagcgact gatatacctt
     2941 tgatgacagt cttgctttca ttactctgtc gtcaaatacc ttacatccaa aataatctga
     3001 tacctttttc ataacaatct cctctccagc agcatatttt taatacaaaa agcaaggatg
     3061 tccttcacag cacctttcct gtgctgcaaa agacatcctt gcctttatgt gtggcataat
     3121 acaccccgaa aaatcatttg tcaagcactt ttttctcctg acaaacctct tgacaaaccc
     3181 tgttcagagc tgtataaata gtgaatatga gcaagggcgt tcatttttaa atcagagaaa
     3241 attctgctta aaaatgaatg tccctgcttt tttatttatt gaaaggaagg tacaaaatgt
     3301 atgaagatcg aaggccaggt gagaatccct tcaggctgtg caatctcggc agtaatttcc
     3361 aaagatggaa ataagatgtc cggcgaaagt atcacaaatt ccatgaagcc tatgcatgac
     3421 cgctccaacg gattgggcgg cggctttgca ggctacggca tttatcctga atataaagaa
     3481 ttttacgcac tgcatatgtt ttttgacacc cgcgatacaa gaaagaaatg cgaagctttt
     3541 ttaaaggaac aatttgagat cgttcaatcc gaggttatcc ctacccgtaa gctcccggcg
     3601 atcacagatg aacctattat atggagatat ttcgtcgcac ccttaaaatc ggttctggct
     3661 tcgatgcagc tggatgaaaa agaatttgtt gcccagacgg ttatgaaaat caatgcggaa
     3721 atgtccggag catatgtttt ctccagtgga aaaaacatgg gtacatttaa agcagtagga
     3781 tttcccgagg atgtaggcgt cttttataaa ctggaagaat atgaaggtta ctcatggact
     3841 gcccatggac gttatcccac aaacaccccg ggatggtggg gcggagcgca cccctttaca
     3901 ttactggatt attccatcgt acataacggt gaaatctcct cttatgacgc taaccgccgc
     3961 tttatcgaaa tgttcggcta taaatgcacc cttcagacag acacagaagt aattacctat
     4021 atcatggatt atctgctccg cgtacaggga cttaccctcg gtgaagccgc aagcgtcatt
     4081 gcggcgcctt tttggagtac catcgcttca aaaacagatc tggaggatca gaaaaaacat
     4141 acctatttaa gaaccatgtt ttccagcctg ctgatcaccg gcccgttctc tatcgtttta
     4201 ggctttaacg gcggactgat ggctctcaat gaccgcttga agctccgctc catggtaatc
     4261 ggagaaaaag atgataaggt ttttattgcc agtgaggaag ccgcgatccg gaccatggaa
     4321 ccaaatgcag aaaatatctg gtctcccgcc ggcggagagc ctgttattgt aaaagtaaag
     4381 gaaggtgcgt tttaatgggt atcgaattta tctatccaga atttgaagta atccgaaacg
     4441 aagaccgctg cacggtctgt cgtgcgtgcg aacggcaatg tgccaatgag gttcacagct
     4501 atgatgagga actgggagtc atgaagtgtg acgaatcaaa atgcgtaaac tgccagcggt
     4561 gcgtttcctt ctgtccaacc agagcgctga agatcgtaaa aaccgactgc tctctcaggg
     4621 aaaactccaa ctggcagaac aatacgatta aagagatcta taagcaggca aacagcggag
     4681 gcgttcttct ttcttctatg ggcaatccca agcccttccc cgtttactgg gataaaatac
     4741 tgatcaatgc ctcccaggta acaaatccct ccattgatcc tctgcgggaa ccaatggaaa
     4801 ccagaaccta tctgggaaaa aagccacaga gagtaagccg caatgcagaa ggaaagctgg
     4861 actgccagct tcctccccag ctggatcttt ccatgcccgt tatgttttct gccatgagct
     4921 atggttccat cagctacaat gcccacaaat cccttgccct ggcagccaca gaactgggaa
     4981 tcctttacaa taccggcgaa ggcggtctcc acgaggattt ttactgttat ggtgaaaata
     5041 ccatcgtcca ggtcgcttcc ggacgtttcg gcgtccatga aaagtattta aatgccggag
     5101 ctgccattga aatcaaaatg ggacagggcg caaagcccgg gatcggaggc catcttcccg
     5161 gggcaaaaat tgtgggagac gtctcacgca cgcgaatgat cccggaaggc tctgacgcca
     5221 tctctccggc cccccatcac gatatctatt ccatcgagga tttgcgtcag cttgttttct
     5281 ccctgaaaga agcttcggaa tacaaaaaac cggttatcgt taaggttgcc gcagtacata
     5341 atatcgcagc aatcgcaagc ggaatcgcaa gaagcggcgc cgacatcatt gccatcgacg
     5401 gtttccgcgg cggcacaggc gctgctccca ccaggatcag ggacaatgtg ggaattccca
     5461 tagagcttgc cctggcggct gttgatcagc gtctgcgtga cgaaggcatc cgcaacgatg
     5521 tatctctggt agtcggagga agcatccgga gcgcttccga tgttataaag gccgttgcgc
     5581 tgggggcaga cgcctgctat gtgggtactg ccgcactgct ggctcttgga tgccacctgt
     5641 gccagagctg tcagaccggt aagtgcaact ggggcatcgc cactcagcgt ccggagctgg
     5701 taaaacggct gaacccggaa acaggaaaaa agcggctggt caacctgatg actgcgtgga
     5761 atcatgaaat aaaagaactg atgggaggaa tggggatcaa ctccatcgag gtactgcgcg
     5821 gaaaccgtct gatgctgagg ggaatagggc ttacggcaaa agaacttgaa atactgggaa
     5881 tctctcatgc aggagaataa tatttctgca aaaaaacgta aacatttttt tggaggtatg
     5941 gtacaatatg ctaatcatcg atgctgcaaa ttcagattat aaagctgtaa atgaaaagct
     6001 ccgcaatacg gatgaggact gtacgatcga gggctgctgc ggacagcgtt ttatcgccgc
     6061 cggtatgtct ggaaaaaacg tcacgatcca cggaatcccc ggaaatgcac tgggcgcata
     6121 cttaaatgat tctcatatta ctgtaaacgg aaatgcccag gacgccgtag gcgataccat
     6181 gaatgaaggg aaaatactga tttatggaaa catcggagac gctgccggtt atgccatgag
     6241 gggcggaaaa atctatgtac agggcaatgc cggataccgc gccggaatcc atatgaaggc
     6301 ttacaaagaa aaaatccccg ttatgatcat cggcggatgc gcgggaagct ttctgggaga
     6361 ataccaggcc ggcggtatta tcatagttct tggacttcac aaaaacggca aaagcattgt
     6421 gggaaatttt ccatgtaccg gtatgcacgg cggaaaaatg tttctgcgcg gagactgcgg
     6481 aaacatacgc ttttcagagc aggtaactgc aaaaacagcc ggggatgagg atctggagga
     6541 aatccgtgaa tatctgtcgg aatattgcgc ggactttggg tattctcttg acgggatttt
     6601 atctgaaccc tttacagtta tcacgccaga cagcaaaaat ccgtacaaac agatgtacgt
     6661 ggcaaattaa aacagaaagc aggtaaggtt atgaaatatt caaaagaaga ggtgctccag
     6721 tatatgcagg aagaggatgt gaaatttatc cgcctggcat tttgcgatgt gttcggcaaa
     6781 caaaaaaata tttccattat gccccaggag ctttgccggg cgtttgaaca tggcattgcc
     6841 attgatgcat ccgctgtcgc cggttttggg gacgaaagcc attctgatct cttcctgcat
     6901 ccggatccgg atacgctgat gcctcttccc tggagaccgg agcacgggag ggtggtaagg
     6961 atgttctgca gtattaccta tcccgacgga accatatttg aatgtgatac gcgctctctt
     7021 ttaaagaaag cggttcatga ggcagagcag gccggatatc agttcttctt tggcgcagag
     7081 caggaatttt atctgttcca tctggatgaa aatggaaacc ccacaaaaat cccctatgat
     7141 catgcaagct acatggatat ggctcctgaa gataagggtg aaaatatccg ccgggaagtc
     7201 tgcctgaccc tggaacagat ggggattttt ccagaaagct cacatcatga ggaaggaccg
     7261 ggacagaatg aaattgattt tcgttattcc gatcctctgg cagccgcgga caattccatg
     7321 acctttcaga ccgtagtaaa gaccatcgcc ggactgaatg ggatttttgc ggatttcagt
     7381 cctaagccac tggaaaatga agcgggaaat ggcttccaca tcaatatgtc tgtgaaatcc
     7441 tctgacggca cagaccatat ggacagcatg attgccggta ttctggataa aatttctgat
     7501 atgactgtat ttttaaaccc caccgctgat tcctacaggc gcttcggctg caataaggct
     7561 cccaaatata tttcctggtc cagtgagaac cgttcccagc tgatccgtgt tccggcggca
     7621 atcggaaggt accggcgtgt ggaactgcgt tcgcctgacc catctgcaaa tacatatctg
     7681 gcgtttgctc ttatgatcta tgctgttctg gaaggcgtac aaaacaatct ggcacttccg
     7741 cctgctgcag acatcaattt ataccgggca gaagcttctg tcctttcagg ctttggtcag
     7801 cttccaaaaa actttgaagc tgcctgtgat gctgcggcgc acagtgattt cattctgaaa
     7861 tatataccaa agaccatatt ggatatctac tgcggacaat agatttttaa caggaggggt
     7921 gaaaatgagt ttaaaagagc gaacctacag tatcctcatt gtgtcggcaa cggatgtact
     7981 cacctctgcc tttgccggcc tgcttcctga aaccagatat tctcctgttc atgctgtttc
     8041 aagtatcaat gccgccagac gtacgcttac agaaaagacg tatgatttta ttatcattaa
     8101 tgctcctttg cacgatgata tgggcgttcg tcttgctatt gatatttcca gcagcaagca
     8161 gtcagttgtc cttcttctgg taaaaagcga tattcataac gaaatccatg accaggccgc
     8221 agaatatggt gtatttaccc tgtcaaagcc aatctcaaag gcaacgatga cctatgcctt
     8281 cagctggctg gaaagcgcca gggagcgcct gaggcaattt gaaaaaaaat ctttgtccat
     8341 tgaagaaaaa atggccgaaa tccgccttgt caataaggca aagtggattc tgatcagcca
     8401 gctttccatg agcgaaccag aatcccaccg ctacatagaa aaacaggcaa tggaccgctg
     8461 tgttaccagg cggtccattg ccgaagaaat catcagaacc tattcttaaa ctttattttc
     8521 tgaaaataat atcctcacgc ccgggaccat tggaaaccat ggtaatgggc gtttcaattt
     8581 ctttttcgat aaattcaata tacctgcggc agttttcggg aagctcctcg tagtttttaa
     8641 taccgcggat gtcgcatttc cagcccggaa gcctggtaaa caccggtttt gccttctcca
     8701 gctttgcagt ggtcgggaac tctcttgtga cctctccatc gatttcataa ccgatgcata
     8761 cgggaatctc atccaaatat cccaatacgt ccagtacggt aaatgcagcc tctgtggtgc
     8821 cctgaatgcg gcagccgtat cttgtcgcca ctgcgtcaaa ccagcctact cttcttgggc
     8881 gtcccgtagt ggctccgtat tcgccgccat cgccgccccg atgccgtaac tcttccgctt
     8941 cctctccaaa gatttcgctg acaaaggcgc ctgcgcctac tgcgctggaa tatgccttca
     9001 caacggttgt gatattttta atctcatagg gtgcaatgcc tgcaccgatc gcgccatagg
     9061 cagccagggt tgaggaagag gtaaccatgg ggtaaattcc atgatccgga tcctttaagg
     9121 aaccaagctg tccctctaac agaatgtttt ttccttcttt gatggcatga tacaaatagg
     9181 aggatacgtc gcatacatag gggcggatca tctcccggta gctgatcagc tcatcaaaca
     9241 gttcatccgc attcaggaga ggtttatggt aaagatgctc cagaagcacg tttttgattt
     9301 cacacactct ctggcatttt tctcttaaca gttcctcgtc gaacagctcg ctgacctgaa
     9361 agccaatctt tgcatattta tccgaataaa agggagcaat gccggactta gtggaaccaa
     9421 aggattttcc agccaggcgg gcctcctcat aagtatcaaa cagcacatgg tagggcatca
     9481 ggatctgtgc acggtctgaa accaggatat ggggcttcgg cactccccgg tctacaaggg
     9541 actggatttc cttgatcaga tacggaatat ttaatgctac tccgtttccg atgatacttg
     9601 tagtatgctg gtaaaacacg ccggaaggaa gcaggtgaag ggcaaatttt ccatattcat
     9661 tgataatggt atgacctgca ttgcttccgc cctgaaagcg tatgataatg tcagactctt
     9721 tcgccagcat atccgttatt tttccttttc cctcgtctcc ccagttagct cctacgattg
     9781 ctctaaccat tgcttcaatc ctccttgggc tgctgccctg atttaaatat cccgcagcat
     9841 agcgggatct cacataagtt ctcttttact ctactacaag ctttcacata agtaaaatga
     9901 ttatttatta tgtgcggcat aagtaaacct tataccattt aggaagattc tgccatttcg
     9961 tcaaacagat ttaaaagctt cctgcctgcc agagaaatca ggctgttctc acccgtaaca
    10021 acacagatgt ggcgcagagg cattttctcc ttaaaatcca gctcaaacac ttctcctttt
    10081 gctatggcat cctttgcaaa tccgtccatg acgcagccta cgcccatatt ccgctttgca
    10141 aactgaacga tcatatcgct ggtagcaagc tcaaactctg gcgtcagttc cattcctttt
    10201 gcagaaagaa acgcgtccat aaaggctctg gtactcgtgt ttttttccag aaaaatacag
    10261 ggcatacttc caagaatact gtaatccagc ttttttcctt tcagttcgcc aaagctgtct
    10321 cctgcaataa aaaccgttcc aatcggtttt accatcatcg aatgaatggt acttttgcag
    10381 gaaaaaggcg ttgtcactac gccaaagtca atttttcctt cctccaggct gcagatggtt
    10441 tctggtgtgg gggcattggt tacggtaact ttgatccgtg gatactcttt gtgaaactgc
    10501 tccaaaaaag gcagaagaaa aaactgaagt gtcatatcgc tggctccgat ccgaacctct
    10561 cccatatcca tatccagcat tctctccagc atcctgccgc cttccagaag ctgttccacg
    10621 cccgttttca cataacggaa taaaagctct ccttcccttg tcagccggat gcccttggag
    10681 gtacgcaaaa aaagccgtgt gccggcctct ttttccagct ggcgcacggc ctggcttacc
    10741 gcaggctggg aaatacacag cttttgggca gccgctgtca ggctgcccgc tgtgcataca
    10801 taataaaata ctttgtaata ttccagattg atattcatgg tctttgaagc atttttatac
    10861 attgatctcc gcagtcatac caagtgcttc tttattggcc tccagtacag gacggatcac
    10921 ttcctcaagg aattcttcca cctgtcttgg tgcacggcct acataccggg aaggttccat
    10981 ggccttttta agatcctcta aggacaatcc gaatgcagga tctgctgcaa tcaactctaa
    11041 caggttgttg tccccgccct gctcttttac attctttcct gcctccatgg aaagctgacg
    11101 gatgcgctca tggagctcct gtcggtctcc tcctgccttt acagcgtcca tcataatgtt
    11161 ttccgtagcc ataaagggca gctctgccat cagatgcttt tcgatgacct taggatatac
    11221 caccagtcca tccactacat ttaagtacag atccaggatt ccgtccaccg ccagaaagcc
    11281 ctctggaatg gacagacgct tattggcaga atcgtccagc gtacgctcaa accactgggt
    11341 agaagctacc agcataggat tcatcatatc gctcattaca tagtttgcaa gagaagcaat
    11401 ccgttcgctg cgcatgggat tcctcttata agccatggcg gaggaaccaa tctgattctt
    11461 ctcaaagggc tcttccactt cttttaagtg ctgcagcaga cggatatcat tggaaaactt
    11521 gtgggcgctc tgggcaatgc cggccagtac gcttaagaca cggctgtcta cctttctgga
    11581 ataagtctgt ccggatacgg gatagcagcc ctcaaagccc atcttttccg cgatccgctg
    11641 atccagtctg cggcagcgct catgatcgcc gtcaaagagt tccagaaaac tggcctgtgt
    11701 gcctgtagtt cccttggagc ctaacaggcg catggatccg ataagataat ccagatcgtc
    11761 cagatccagc tttaactcca tcatccagag ggctgccctt tttcctacag tagtaggctg
    11821 agccggctgg aaatgggtaa atgccagagt gggcagattc ttatatttat ccgcaaacgc
    11881 tgccagctcc gccagtacat tcagcagctt tctgcggacc agctttaatg cctctgtcat
    11941 aatgatcaca tcggtattat ctcctacata gcaggaggta gctcccagat ggatgatgcc
    12001 ctttgccttc gggcactggg tgccatatgc gtacacatgg ctcatcacat catggcgcac
    12061 ttctttctca cgggcctttg ccacatcata gttgatttca tctgcatggg ccttcagctc
    12121 atcaatctgc tcctgggtaa tattaaggcc tagctccttt tctgtctcag ccagagcaat
    12181 ccacaagcgt ctccatgtct taaatttcat atccggtgaa aaaatatact gcatctccct
    12241 gctggcataa cgctctgaaa gcgggcttac atacgtatca tgattgctca tgtcttatcc
    12301 tccatctgtt tgttataagt tttatatata aatatgctat gattcagaag aaattataat
    12361 actttcttat tttgaacaca agacagacag tcattaaaat tcctaataaa tcatctctgc
    12421 ccttctaatc atcacaggct tcctcgtgaa tccgaaaaaa tactatgcgc ccttctttta
    12481 aaaacaggga aatcccctgc tctgtccggc tggcgttctc tttctccctg cgaaattctt
    12541 cttcataaaa gggatggatt tctatgaatg cgggcaggct gtctatacgg atttctgctg
    12601 tgcggtttcc agactgccag tcctttgccg aggccgtctc cagacagata cagacaaata
    12661 taagcatccg aacccccaaa cgcccttttt ggtctttttc ctgccgcttt tcgcgctgcc
    12721 tgttttttct tacagctttc catctgtata cggccttttt acgtcttctt tttaaacagc
    12781 ggttccgttc tcttttgttc atggtttcct ttcccacgca ctcctgactg cattatagac
    12841 gtaaatccgc ctgactgcaa caaaaaaatc tccctgaagc ttcgaaagct gtatctgctc
    12901 tccaaatgct cagggagatt ttccgtctgt cctccggttt taaatgattt tatttaccgg
    12961 ttttgtgtct cggctgtgga ctcagattca gttgtatcca ttatatccct ttttatgtcc
    13021 tctacgcctt ttctcacatc atctgcaatc ccgtccatga ccccttcccc ggtggtatct
    13081 gtttcagacc gggatgtggc gtccgtattt tcagtttcat ttgcggctgc gccctggtct
    13141 gtctctgtgc tgtaggaggc agaggttccc ggtgttcccg aagaagctgt aggctgcttg
    13201 gaatctctgc tgccgcatgc tgtaagggat gatgcaagca cggcaatcaa cagtaccatc
    13261 ataacaggtc ggatcttttt catcatacat tctcctttcg tgctccaggc atcgttttct
    13321 gtccggtgcc ctattaaaaa agtgtatcca aaccttacgt tcagatacac ttagtataag
    13381 ctcccaaaaa agttttattc ctgtttacag accgatcaca tctgtcatgg tataaagtcc
    13441 cggctgtttt ccggccagaa actttgccgc ctctaaagct ccttttgcaa aaatggcttt
    13501 tgaataggcc gtatggctga tcgtaataac ttcatccttt ccagcataga tcatatcatg
    13561 ttctcctacg atcgagcctc cccgtacaga ggaaataccg atttcttttc catctctttt
    13621 ttcgtggata ctgcttctgt ctgttacgaa atgatacaga ccgcccatgg cttcattcac
    13681 cgcttctgcc atcgcaagag cagttccgct gggagcatcc tttttctggt tatggtgctt
    13741 ctctacgatt tccatatcaa agcccgcatt tcccaatacc tttgccgcgt cctgaatcag
    13801 ctttaaaagc agatttacac caagagacat attggcagag cgaagaaccg gaatcctttt
    13861 gctggcatcc tctgcccttt ttaactgttc ttctgtaagt cctgtggtac acagcaccag
    13921 aggaagctct ttttccagac agtaatccaa aagttcgtcc actgcctttg cagaagcaaa
    13981 atccaccact gcgtccgcag cctctgtaca ctctcccaaa gaggcatata ccggataatc
    14041 ataatttttt tccgtattca gatcaacgcc cgcagcgatc cggattccgt ccatttcctg
    14101 tgccatggcg gaaatcaccc gtcccatagc gccgttgcag ccgtgcatga taagattgat
    14161 cattttctgt ggtatccttt ctttactgag tcttcttttg gcaaggccca gggctctttt
    14221 caaacgtttt atgccagaat gccgtattcc ttcatggcct ttttaagaat ttcctgatgc
    14281 tccggctcca tctcggtcag aggcatgcgc agcggtccta cttcttttcc catcaggttc
    14341 atggcggcct ttacggggat aggatttacc tcacagaaca gggcgttaat taaaggaatc
    14401 gcctctaact gcatccgggc gctctttttt acatctcctg caaaatagga ggcacagatt
    14461 tcatgagcct gtgaaggagc cacattggag agaacagaga taacgccctt tccgcctaaa
    14521 gataaaagag gcacgatctg atcgtcattt cctgaatata cgtcgcagtt tccatcggtc
    14581 aggttcatca gctgggcaat accggaaaaa ttgccggtag cttcctttac acccactata
    14641 ttgtctactt cacggcaaag agaagcaatg gtctcaggct ggatatttac accggttctt
    14701 ccgggaatat tgtaaaggat catgggtatt tttactgcat ctgccacagc agtaaaatga
    14761 gccttgagtc ccttctgggt cgccttgttg taatagggag atacaaccag gataccgtct
    14821 gccccccttt cctcggcctc ctttgacagg taaatggcgg tctgtgtaca gtttgagcct
    14881 gttcctgcca caaccggaat tctctttttg gtcacatcac agataaagcc gatcacatcc
    14941 agatgctctt catgggtcat ggtagatgcc tctcctgtgg ttccgcaggc aatgatcgca
    15001 tcggtaccgc ctgcgatctg ctcctctacg atctcctcca gcttatcgta atttacttct
    15061 ccgttttcct taaaaggcgt aatcagcgca acgccagctc cttcaaaaac agccatgctt
    15121 atcttctcct tttttacagt cttatttacc atcagtaaat gcgcatgata aacaaagtgt
    15181 acatatcccg caccggtttt cgcccccgta ccagcgaaaa aagaagggct gaggtaaatg
    15241 tttcagccct tcccatacaa acccgttctt actgcggata catatcttct ggtagctctc
    15301 catccacctg gatgacagtc acaggattct tcatcctgca cccagaaacg atcctgcagg
    15361 ataaccgctc cttcggcgct tttccctttc acggcttttc ttctgtgcct gccacatcca
    15421 aaccgatatt catgatcagc gcagcctcta ctctgtctat agtcttatcc tatcatcatt
    15481 aaaaatagat gtcaacaggg aacttactca aatcgcgtct gaagcttcgt cacctcatcc
    15541 acccattcat ccaaagccgc cggataaacg gttcctgtca tgatcagctc ggcctccgac
    15601 tgtcttgcct ggccgatcag ggcgcacagc tcctccaggg tgagaatccc ttcatccagg
    15661 atccccagga tctcatccag gatcaggata tcgcattctc cggttacaag aacctttttt
    15721 gaaaaattca gcccattgcg gatattgatc ctggcttcct ctttttcctc ttcagaaagc
    15781 tgatcaaaaa ccacaggaga tttttcaaaa cggaacagtt taaattccgg ttccagacgt
    15841 tttaatactt ccatgttgtc ggaatccata gaacccttta aaaactgaac catgatcaca
    15901 ttttttccat ggatcagggc gctgatccct cttcccagag cgctggccgt tttcccctta
    15961 cccggtccgc agatcacctg tatcgtactg tctttcatca tcatgcacct catactgcct
    16021 tccggacacc ttccctatgt gtgtctggaa catcaattca caacacacac tctagcacaa
    16081 tataagaaat aatgcaaata tttcttatat tttccgtaag gaaatcgtaa gagaatttat
    16141 tgatccgact cctcgccgca ttccagcttg ctgatagaaa cctcgtatgc caccctcttt
    16201 tcacactctg tctcgctgag cttttttgta tattccctgc tctggactct tccccagata
    16261 cggactcggg tgcctaccgt aaagccggag gcaaagcggg catttcttcc ccagctgata
    16321 caaggtatgt aatctgattt tccataagga cggttaactg ccagcagaag atctgcaatc
    16381 tctcgtccaa gaggggtttt tcggtagatc ggaggcttgc atatgtaacc gtccaggaag
    16441 atctggttgg ttctggtgta atccgtaaat tcatccagaa aacgaatctc tctggcaaaa
    16501 acagacagca tcaggcggtt ttttacaccc tcatggcggt tataggaccg gaactgcccc
    16561 actgcctcga tggtgctccc ccgcagatcc ttatggacat ccataagacg ttccgacacc
    16621 ataagcggaa ttacatcggc ctgttcgctg agacggttta ccgacagatc caccatgtaa
    16681 aacccttcgc caaatacctc gtggctgaag gagaactccg atacgatttc tccgatcacg
    16741 ctcactctat tgttttcaat cattttttct gccattgtac ttctcctctt attttccaat
    16801 ttcactccgg gttacaattt tcccgtatgc acataatcaa tctatgcaat tataaccaga
    16861 aatagacgga aaacaaaggt caattcatcg catcgattcg acaactcagg aaaataacgt
    16921 cgattttagc aaaaaaatct gtaaaacgca aaaaatctcc cggactttgg aagaatattc
    16981 caaaaattcc gggagaactg tcatatttta tttaaaaaat aaattttatt ttttgaagga
    17041 agctctcttt cgtaaaagag gatccaggat ctttttacgg atacgcaggc tctccggggt
    17101 tacctccaaa agctcgtctg tatcaataaa gtccaggctc tgctctaaac tcatgatctt
    17161 tggcggagtc agctttaagg cctcatctgc actggaggaa cgagtattgg tcagcttctt
    17221 tgtcttgcag acattgatct caatatcctc tgccttgggg ctctgtccca caaccatacc
    17281 ggaatatacc tttacgcccg gcccgataaa gagggttcct ctctcctgag cattaaacag
    17341 gccgtaggta atggcctcac cggcctcaaa ggcgatcaga gagccggtag gacggtagga
    17401 taagtcgccc ttgtactctg catagccgtc aaaaattgta tttaagatac catttccctt
    17461 ggtatccgtc ataaagtcgc cgcggtaacc gatcaggcct ctggacggga tagaaaattc
    17521 cagtctggaa tagcctccgg ctgcctggct catgccctgc agggcgccct ttctggctgt
    17581 cagcttctgg atcaccacgc cggtatactc ctcaggaaca tcaatataag caagctccat
    17641 gggctctaat ctgcggttgc gctcatcata gtgataaagc acctctgctt tacttaccgc
    17701 aaactcatag ccctcgcggc gcatattttc gatcaggacc gacagatgaa gttcaccgcg
    17761 tcctgatacc ttaaagcagt ctgcagagtc tgtttcctct acacggaggc tcacgtctgt
    17821 attcagctcg cggaacagac ggtctcttaa atgacgggag gtgatatact ttccttcctg
    17881 tccggcaagg ggactgtcat ttaccatgaa gtacatagca atggtaggct cagaaatctt
    17941 ctggaagggg attgcctccg ggttctgtgg agagcagatg gtatccccga tatggatatc
    18001 cgcaataccg gaaatggcaa cgatggagcc gatttttgcc tcctgaacct ccacacgctt
    18061 taagccgtca aattcataaa gcttaccgat cttgatcttt ttaaatttgt caggatcgtg
    18121 atggtttacc agcacacact cctgattcac cttaaggccg ccgttttcca ccttgccgat
    18181 accgatacgg cctacaaact cattataatc aatggtactg atcaatacct gggtgtccgc
    18241 atccgggtcg ccttccggtg cgggaatgtg gtttacaatg gtctcaaaga gaggtcccat
    18301 atccacctct ggatcgtcaa tattaaattt tgcaaaaccg gctcttgcag aagcatagat
    18361 aaaggggcag tccagctgtt catcggaagc atccagatcc atcataagct ccagcgtttc
    18421 gtcgatcacc tcttccggac gtgcttccgg acggtcgatc ttattgatgc acacgattac
    18481 ggacagattt aactccaatg ctttctgaag cacaaacttg gtctggggca tggggccttc
    18541 gtaggcatcc acaaccagaa caacgccgtt taccatctta agcacacgtt ctacctcgcc
    18601 gccgaaatcg gcatgtccgg gagtgtcgat aatattgatc tttgtatcct tataaaatac
    18661 agctgtattt tttgacagaa tggtgatgcc tcgctcacgc tcaatgtcat tggagtccat
    18721 gacgcgctcc tgaacctcct gattggctct gaatacgccg ctctgcttta acagctgatc
    18781 caccagagtg gttttgccat ggtctacatg ggcgataatg gcaatatttc ttacatcttc
    18841 tcttttcatc ttcatagtga tcattcttcc ttatttatgc agaaaactta tagatttagt
    18901 cacggtttta tagtatagca ttggtatctt aagattgcaa gacccaaatc caggtgcatt
    18961 ttaaaatacg atcagctctc cccggttcat gccatagtac agttcttttc cggtgcgtat
    19021 cctcgcccgg ccgttggtcc cttctgtgat cttggcctgg aagctgtcct ttacggcaac
    19081 cggtaccaga aaatcaaact ctgccttatc ggaatacctg ctttccagag tatcgatccc
    19141 ctcctgggcc gcaatatact ggatctttcc cacgctgttg tagtcggctg taacctccag
    19201 ctgcactccc agcttttttt ctacaaccga acagtttaaa agcgcttctt ttacagcacc
    19261 ggaataagct cttacaaggc cccctgtccc taaaagtgta cctccgaaat accgggtaac
    19321 tactgcacat acatcgtgga gcccctctcc tgccagcaca tccatcatgg gctttcctgc
    19381 cgtctgggag ggctcgccgt catcgctgca ccgggccgtc tgagcccgtt ctcctataat
    19441 ataggcgtag cagtggtgcc ttgcatccca gtatttctta cggacctcct caataaaggc
    19501 aaaggcttct tcctccgaag ccacgggacg gatctgggca ataaaccggg actttttttc
    19561 caccagctcc ccatctgcgc cctcatatac gatcttgtag ctttctacca cttttctctc
    19621 tccttttccc tttttttcag aaactgcacc cacattttcc ttatttttct tctatttttt
    19681 atcataatat agttaggctt tcatggcaag ccatatttat gccttttcaa ttccataaaa
    19741 ctgttgtata atttaatcgt ctgccaaatg cctcagggct ttgtcatacg gggggaactt
    19801 gttgtaaagg agaaactatg aattacggaa cacatgagtc tgagcgcagg cttagggctc
    19861 tccagtcaaa atcggccaaa tatacctcaa agctgttttt taacatcttt aaggcttttt
    19921 ttgtattgtt tctttttctg gccatcgcag gcactgccat cggtttcggc atggtaaagg
    19981 ggattatcga cagcgcccct tccgtagacc ttatgaacat ccagccggat cgctttgcca
    20041 ctaccgttta tgacataggc ggcaatctca ccgacacaat tgtaaccagc ggttccaacc
    20101 gtgaatctgc tgcctatgag gaactgcccc aggatctgat cgatgccttt gtagccattg
    20161 aggattcccg tttctggtcc cataacggta tcgatatccg ttccatcgcc agagcggtca
    20221 acggcctaat cagcgacgac tatgccggtg gcggaagcac catcacacag cagcttatca
    20281 aaaacaatat ctttgccggc ggtatggaaa ccagctgggg agcccgtatt gaacgaaagc
    20341 tccaggagca gtatcttgcc gtacatctgg aaaaaaattc cggcatgtcc aaggaagaaa
    20401 caaaaaaagt cattattacc aattatctca acaccattaa cctgggctat aatactctgg
    20461 gtgtaaaggt ggccgcacgg cggtatttta ataaagaagt atctgatctt accctttccg
    20521 aatgtgcggt tctcgcctcc atcacgaaaa atccctcccg tctcaatccg atctccggcg
    20581 ccgaggctaa tgccgaacgc cgcagcattg tccttcaata tatgtatgat caggattata
    20641 tcgacaaaca ggagctggaa gaggcgctgg cagacaatgt gtatgagcgg atccaaaacg
    20701 taaatacagc cgcacaggaa acaaactccc atttcagtta ttttaccgat gagctggtcg
    20761 aacaggtcat tgccgctctt atgggggagc ttggatattc ggaaagccag gcgagaaatc
    20821 ttctttatgc gggaggactt aaaatttata ccacacagga tcccaagctt caggccattg
    20881 tggatgagga gatcaacaat ccggaaaatt atactgctgc caagtattcc gtagaatacc
    20941 gtctttccat cacacatacg gatgggacaa ccgagcattt ttcagaagaa aatttaaaat
    21001 cctaccacaa gtctgtattg ggagatacgg gctatgatgg cctttatcca agcaaagaag
    21061 cagtccagga ggatatcgaa cgctacaaag catggctgat caaggatggc gacaccatta
    21121 tcggagaacg ccagaacctg attttacagc ctcaggcgtc ctttgtactg atggatcagc
    21181 gtaccggtga ggtacgggcc ctttccggag gacgcggtga aaagaccgcc agcctgacct
    21241 tgaaccgtgc cagcaatgta taccgccagc ccggttctgc atttaaggta atcactgcct
    21301 tcgctccagc tcttgacacc tgcggagcca ccctgggaac ggtttattat gacgctcctt
    21361 atacgattgg aacaaaaacc ttccgtaact ggtggggttc cgggaaaggc tttgtaggct
    21421 attctaatat ccgtgaaggc atcatttact ccatgaatat cgtagcagtg cgggctctga
    21481 tggaaaccgt aacgccccag cttggcatgg aatacgctga aaaaatggga attacttcca
    21541 tgacaaaaaa tgatctcggc gctgcccagg ctctgggcgg actcacaaag ggcgtttcca
    21601 acttagaact cactgccgcc tatgcctcca tcgcaaacgg cggtgtgtat acaaaacccc
    21661 ggttctttac aaagatcctg gatcatgacg ggaatgtgct catcaacaat gagcctgaaa
    21721 cacggcaggt attaaaggat tccactgcct ttctgcttac agatgccatg gcggattcca
    21781 tgaaagcaaa ccggaaattt gcgcgttccg gcgtatccat caattccacc agtaccagag
    21841 cagccctgtc tggaatgtct gcagccggca agagcggcac taccaccgga aataatgata
    21901 tctggtttgt aggttttact ccctactata cagccggtgt ctggggcggc tgtgacagca
    21961 atcagaaact ctctgccgga aacggaggta cctctttcca taaggatatc tggcgcaata
    22021 tcatgaaccg tgtccatgag gggctttctg atcccggatt tcccgttcca gacagcgtgg
    22081 aaacggccga aatctgccgc aaatccggca aacgggcagt acccggcgtc tgcgatcacg
    22141 acccaagagg cagcgccgta tatacggaat actttgctaa aggaaccgct ccgtccgagg
    22201 tctgcgacaa gcatgtggct gtaaccgtct gcgcccagtc cggcctgcgc cccactccgt
    22261 actgcacgga aaagacaaca aaggtatgca tggtactgcc tgagggagag gaaaatctgg
    22321 caacggatga ttccgtattt tccataccag gtacctgcac cattcattcc ggcaattcta
    22381 ccattatcgc accttccttt ggctcggatg agactggtgg ctccggcgaa aatgtaacca
    22441 tctctcccat cggccccggt tatgagccac aaacccgacc gcagtgggaa aattcggggc
    22501 ccggcgtccg acagaactga tatttaaaga gaataaaaag aatctgtgaa tacagctgta
    22561 ttttacatac tgtttcacag attctttttt tgcagcgtca agctggccct ataggatctt
    22621 ccgccgtctt actctgcaat ataatatccg tcccgcccct gttccttcgc cttataaagc
    22681 gcccggtccg ctctttcata aagatctcta aaacccatcc ccttttctgc ttctgtaatt
    22741 cccacagagg caccatgatc atgatctggc tggtatcctg ccggccgctg gtaaacacat
    22801 cagaaacata agctctcccc ttttccatag cagccaaaag ctcactgtca tctatctgcc
    22861 agcggtttcc cgtatcgtct atggtattcc cttcctgatc cataatgcct atctgtgcaa
    22921 atcccgccct gctcctgatt tcctgaagtt gttttacgat ttccgcggaa gtcattttct
    22981 ctggtacgct gaaattttcc tcacctatat ttaaaatctc tgtacaatgc ctcatctcac
    23041 ttcgtataat ctttacaata tagtcagata aaagggaaat ctgctccata ttacggttaa
    23101 gaatttcatt atgtatacgg ttggaatagt gaaaaaacag cgaaaaaata ttgataacgg
    23161 aaaaagaaac aataccagcg atcaaaagaa tccaggctgc agttttatag cggtttgatc
    23221 tcttgtcttt catatatctg cctcatataa tattttaaaa atacgttttt ctaaaaagaa
    23281 cccgccagtt ctctcccggc gggatttttt ccaattatac cagattgcca gggacatatg
    23341 caatagtttt aaagtattaa acttcccctc acttcatctt gggcttaaaa agtacggaag
    23401 ctgaataccc gaatatgaga gccccggtga ttccggcaga tgcggcggtc agcccacctt
    23461 taaataatcc caatactcct tcctctgcca gcgcagtttt taccccctta aaaagcgtat
    23521 ttccaaagcc cagaagggga atgctggctc cggctccggc ccaggaaaga aagggctcat
    23581 aaatccctac cgcccccaga atggctcctg aaacgactaa cagtaccatg atccgtcccg
    23641 gcatcagttt cgtcttttcc atcaggatct gaaccagagc acagataatg cctcctgtaa
    23701 caaatgcttt tatataatcc atatatctgc tccttattta tgctggatcc ggcctttagg
    23761 gcctccccca ttattccaaa tgctctatta ccaccccatg ggcaattccc gggatggtct
    23821 gtccttcgtt gtaacttacc gtagaaagaa gagcgcctgt aggcacaaaa agaacacgtt
    23881 tccagattcc ctcccggatt tttggaagga tcgccgcgca cagcgtaacc gcagaacagc
    23941 cgcagccgct tcctcctgca tgggtatcct gggtttcttt gctgtaaatc tcaacgccgc
    24001 agtccatatg gcgttccttc aggctgcgcc cctttttctc catcagatcc aacaggatct
    24061 gcgttcctac ctctcccagg tctccggtga tgatcctgtc ataccaggtc tcatccgttc
    24121 caagatcctc cagattctgt tcgattgtat gaaatgccgc cggagccatg gcagctccca
    24181 tattcataga atctctggct ccaatatcga tcgcttttcc ggcagtaaat gccgtgatct
    24241 ttgccagtcc cggctgcccc tctttcccaa tgacgactgc cccgcaccca gtcaccgtcc
    24301 aggtagaaga catagggcgc tggttcccgt aggcaagagg aaaacggaac tgtttttctg
    24361 cggtggcaaa atggctggac gccatggata gcacatgatc cgcatagcct gcatttacca
    24421 tcattgctcc caggctcatg gcctctccca tggtggaaca ggccccgtaa agcccaaaga
    24481 ggggaatttc cagatctacg gttccaaaag aggtggcaat cagctgtccc agcagatctc
    24541 ctgcaaaaag ataacggata tcatcccgtt tcagtccggc ttttgtaatg gacagatctg
    24601 cagccgcctt ctgcatggag ctctctgcct gctcccagtt ttccatcccc tttccatcat
    24661 ccatgatatg acggtcaaaa aacggtccta agggcccttc actttccttc tttccaacta
    24721 tactggctgc tccgcagata accggaggcc gtgcaaacgc taccgtcgct tttcctttct
    24781 tctgttccat ctgctatgtg ctcctttccc atatcagacc gtattttcca ttgaaaacat
    24841 ccggcgattg gaaatattat gcgtaaacag atgtgatttt atgcaaatga cagaaaaagc
    24901 atcctgttaa aaaatacctt cattgacaga tcacagtctc gactttatac taaaaacagc
    24961 atagggaggt gctatactta tggatcttag taataatttt tcagtctgga accacctgac
    25021 cccggcccag caggagctta tatccaccca tcttatctgc cgggatgtga aaaaaggcac
    25081 tgttttgtat aatggaagct ctgactgcct gggactcgta cttataaaaa aaggccagct
    25141 gcgggtctat atgatttccg aggaaggacg tgaggtcacg ctgtaccggc ttttggagcg
    25201 ggatatctgt cttttttctg cttcctgcat tatgaaaaat atccagtttg acattacgat
    25261 cgaggcggaa aaggattcta acatctgcat cataccagca gtgatctaca aacgtctcat
    25321 ggaggaatct gctgctgtct ccaattatac aaatgagatc atggcagccc gcttttctga
    25381 ggtgatgtgg ctcattgaac agatcatgtg gaaaaccttt gaccggagac tggcctcctt
    25441 tcttttagag gaaatggctc tggaaggaag ttccggatta aaaatcaccc atgaaaccat
    25501 tgcaggccat ctgggtactg ccagagaggt agtaactcgg atgctgcgtt acttccagga
    25561 ggaaggcatg gtaaaactca gccgggggat gattaacatt accgatgaaa aacgacttag
    25621 aaaactggcc gaatcataaa ataagccaga cgcaggtaat acgaacgcgt ctggcttttc
    25681 gatcacagat atctattttt taccattttt ccctttgcag atcagctggg tcatggttcc
    25741 catgggacag tatacgcacc agctgcgggg cttaaacagc accatggtaa tgagtcctaa
    25801 tatggtagag gtcagcatca cgctgtaaaa tccaaaggca aactgggctg tgccgggatg
    25861 gaacagggtt ccgtggtagg cccagtgcca tggaagctta aatatccaca agagcgtcac
    25921 aacctgcttt aaatcagaag ctcctgtaaa taccagaacc gtattccaca gcatctggca
    25981 gaacataatg aggaagaaca ccagaaaacc gtatcgaaac gccctgcttt tcatccatcc
    26041 cggaatgtcc tttttcctgg aaagccccag ccggccgcct aacagcccaa aaagctgccc
    26101 cctgccacag tagcggttac agtagccttt attcccctgc accaatgcaa tgatcagggg
    26161 gataaaaaag cataaaagtc ccagccatgc aaacataata ttgaaaaatc ccaggatcag
    26221 ataggtcagg gacaaaatcc acatataatc ataccagtgt tttttcatca tcccacctcc
    26281 ctgatctcaa tcacggaagc cgggcatttt tttgcacatt ttccgcagcc tacgcatttt
    26341 tccaaattca cctgagccca cattccatct gccacataga ttgcccctaa gggacaaacc
    26401 tttacgcagc agccgcaggc cacgcacagg cggctgtcta cggatgcttt ccttttcttt
    26461 ctaactcccg ttgccatatt cttcctccgc tctcagtttc tatcggccgc cggccaatac
    26521 cgaaacggct taattttata tcgagtatag cggattgcgg gaaatatgtc tgtaattaag
    26581 tcacatataa cacaccccca gctcagacac caggatttta tttaactttc ttctgtttcg
    26641 tggctatttc cattctttcc atatatccgg ttcatagcct atggctgcct gttttccatt
    26701 acggacgatc ggcaccttaa ggatcttctg attttccaga atcttttctg ctctgtcttc
    26761 ctctgcaata taccggatca gagccaacag atccttatcc ctgcagtccg gatcaataag
    26821 cttttcataa cctcccacag cccggcatac agcgttaaat tctcctttgc tgagcccttt
    26881 ttccttcata tctataaatt ggtatttaat cccccgctcc ttaaaatacc gctctgcttt
    26941 tttactgtca aagctctttt tcgttccaaa aatctgtata ttcatgcgta tcctttcacc
    27001 tttttatctt ctcccaaacc gttctgcgat ccggccgtca cggtaggcct ttaacagctg
    27061 ttccagatct gcgatttttt ccttcagctg ctgtcccgtg tccgtatctc ctaccagctt
    27121 ccggctggct gtccgtttta caataatatc ctccgaatag atatcgcttt cctgttcaat
    27181 ggcggctgcc ctggactcaa agggttcatg agctgccagg atcaggccgt aggaattata
    27241 aatcagggta tatccggcga ttcccgtctc tttctggtaa gctctggaaa agccgccgtc
    27301 aattaccaga acctttccgc cgcatttaac cggattttcc ccatttttcg acttgacagg
    27361 aacatggcca ttgatgatat ggctctcctc tccgcccagc ccaaattctc ccaggatcat
    27421 atctacggtt ttttcatttt caagcatccg gtaataggga tttttctttt ctttatggct
    27481 ttcctcatct tccagaaaat accgttcaaa ggtagccatc ttatcctttc caaaaagcgg
    27541 ggaattttct cccagccata tgtaccacat gatgtcccgg cccctttttc gttccgtctc
    27601 atcctccgcc ataaagcctc tgcggatata gctttccagc acatcataaa gtcccctgcc
    27661 tttatagcgc tttccgtaaa cagacgtttc ctttaagctt ccgtcctcac aaaggggaac
    27721 gcagccgtga tagaggaggt tttgattata taccttatac aggcttccct ttgccagaag
    27781 aaaccgcata tgctgctgaa gcttttcaca gtttaaaaat gccttttcca gccgttccat
    27841 aatggactct tcctcaggag aaagccggta gggatctgca tgatccaccg tagggaaatg
    27901 catatccttc atgggcagac gtcttccatc cagaacaatg gtgccatttt cataattgat
    27961 ccggtgaaga aggaggcggc gttccagacc aaactccgga tactccttga tcagctggcc
    28021 ttctacctta aactggataa tggaaatggc cttgtgcata cgcagattca gttccttttc
    28081 tccgggactg catggatggc ttccctttaa ggcaaagcag atgcagggat catccccata
    28141 ggtttccagg gcaaaggtag caagaggaag cagattgatg ccataaccgt cttctaaaat
    28201 atccagattc ccatatctgg cgcagatacg gatcacattt gctatacagc ccctctgccc
    28261 tgcagccgct cccatccaga gcacatcatg gttgccccac tggatatcca gagagtgata
    28321 ctgcatcagc ttatccatga tcaggtgcgg accggggccg cggtcataaa tatcccccaa
    28381 tatgtgcaga tggtcaacga ccaggcgctg gatcagctca cagagagctc ttataaactc
    28441 ctccgcccgg cccacccgta taatcgtggc gataatggcg gtgtaatagg attctttgtc
    28501 tcggatatct gccttttccg tcatcagctc ttcaatgaca taggcaaatt ccggcggcag
    28561 cgccttgcgc accttagaac gggtgtattt gctgccggaa cgcttgctga cctctataag
    28621 acggtaaaga gtaatcttat accagtcctc cttatttgcc gtgtgctcta aaatctgcgc
    28681 cattttctct ctgggataat agatcagcgt tgccagactg cgcttatctt ctctgcacag
    28741 cgtattccca aacacgtcat ttaccttgcg ccgcactgcg ccggaaccat ttttcagcac
    28801 atgggaaaat gcctctgctt ccccgtggat atctgtaagg aaatgctccg ttcccttagg
    28861 aagatttaaa atggcctcaa ggttaataac ttccgtagaa gccgcagcta ctgtaggata
    28921 aagctctgca agccgttcca gatatcgggt ttgaagatct gccatgaata ccctcctttt
    28981 gatggatccg ttatttttcc gtatgctcta taaatgttcc ctctatggga tctatatggt
    29041 ctaattccct gtaatcatct gtacgggcag aaagcttttt tcttctgagc atcccgcaga
    29101 tatattcaga gatcaaacga taaaatactg caaataccgg taccgctatg atcattccga
    29161 caaagccgaa gagtcctccg aacagcagga tggaaaacag cacccagaaa ctgggaaggc
    29221 cggtggaatc tcccaggatc ttcggcccta aaatatttcc gtcaaactgc tgcagcacca
    29281 gaataaagat caggaaatac agacatttta aagggcttac aagcaggatc agaaatgcgc
    29341 tgggaatggc tccgataaac ggaccgaaaa aggggatcac atttgtaaaa ccaataataa
    29401 cgctgatcaa aagcacatag ggcattttta gaaaccgcat tccgaaaaag cacataatgc
    29461 cgatgatcag agaatccaga agctttcctg taatgaaacc gccaaacaca tggtgggcaa
    29521 agcggatctc attaatcagc cggtttgcca tgcgaacact gaacaggcca tagattattt
    29581 tcttaaactg agcgcaaagt gtccacttta aattcagcag atagatcatt acgatcagac
    29641 cgatcaaaat atttttcacc actgtaaccg cattgatcac tccggcagat acatgatcca
    29701 ggatcaaata aatacttggc accagatctg tcgtcatcca gtgctgaaat tccatggccg
    29761 cctcattaaa atgaggcatc acgatttctt ccagttccgg attatcttca agaagctgtg
    29821 acagccataa tgtaaggtta ttgatatatt cggccaggct ttcctgaaat ccgagaatgc
    29881 tctccatgat ctgtgggata atcatccaga ccaagccgca gaccaatata agcagaaaca
    29941 gcagactggc aattgtagct gctgtttttc ccgctctgct tctgactttt tctgtggtaa
    30001 acagcttttt cgtattacgg cagacaaaga aatagaccga attatagaca ggaagcatca
    30061 agtatgcgag aacgcctcca taaataatag gcatcagaat ctgaatgatc tctccgatca
    30121 ctccaaaaat agtctgaact ttcatcaggg aaaaagccag aaaaacactg caggcaataa
    30181 cagccaatgc tgtaagcccc cagttgatat aaagccggaa tctttggttt
//
LOCUS       DBMDUH010000010        29841 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_1142_length_29841_cov_6.682011, whole genome shotgun sequence.
ACCESSION   DBMDUH010000010 DBMDUH010000000
VERSION     DBMDUH010000010.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 29841)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 29841)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29841
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1142_length_29841_cov_6.682011"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /gene="fliB"
                     /locus_tag="ACZPCG_01980"
     CDS             <1..948
                     /gene="fliB"
                     /locus_tag="ACZPCG_01980"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF038110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin lysine-N-methylase"
                     /protein_id="HIR1283874.1"
                     /translation="SFLRYEGRCAFLNEENLCDLYIEQGKDSLCRTCRQYPRHTEEFE
                     GCREISLCLSCPVAAELILGCKEPVRFLEKEDEREETYEDFDFFLYTKLIDARSLIFK
                     LLQDRKTPVSLRLSMVLALGHDFQGRIRRNRLYEADALFARYEREDRIRYFEEKKIQG
                     SRYDFLKKLFSLLGEMEPLNLKWPSRRGKIEGSLYGSGEEAYKKTWNVCLKACPELEL
                     WTEQLMVYFVFTYFCGSVYNENPYGKLKMALASVLIIQDMALERFMEQGSLDVKAMAD
                     TAHTYSREVEHSDENRLLLEERLTKDPRFGLRDFLGAML"
     gene            1079..1915
                     /locus_tag="ACZPCG_01985"
     CDS             1079..1915
                     /locus_tag="ACZPCG_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HIR1283875.1"
                     /translation="MSECNHDCGSCSANCESRTTDKTSFLEALAPESKVKKVIGIVSG
                     KGGVGKSLVTSMLAVSMNRKGHHTAILDADITGPSIPMAFGVADERPAVTPDGRLMIP
                     AKSLEGVEIMSANLLLENATDPVIWRGPVIAGAVKQFWTETLWQDVDYMFVDMPPGTG
                     DVPLTVFQSLPVDGIIIVTSPQELVSMIVAKAVNMAKKMDIPILGVVENMSYLECPDC
                     KKHIHVFGESHVEQAAQENGLKVLAQIPIDPKIAQMVDGGRVEYIEMPWFEKAAEAVE
                     AL"
     gene            1939..2652
                     /locus_tag="ACZPCG_01990"
     CDS             1939..2652
                     /locus_tag="ACZPCG_01990"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HIR1283876.1"
                     /translation="MNTNLSPNSELHQSIVRVPDVAQVPDIWLDQARQFQQVMMHYTC
                     AIREVKTKLEVLNDELSVKNQRNPIEMIKSRVKKPLSIVEKLQRRGLEVSLESMVKNL
                     DDVAGVRIICSFVDDIYEVAEMLVRQDDVKVIAVKDYIKNPKPNGYRSYHMIVEVPVF
                     FTDSKRFIRVEVQIRTIAMDFWASLDHELKYKKSFVDTDGEISGELKECADVIAQTDE
                     KMLEIRKRIEAQGVEVRRD"
     gene            complement(2669..3406)
                     /locus_tag="ACZPCG_01995"
     CDS             complement(2669..3406)
                     /locus_tag="ACZPCG_01995"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1283877.1"
                     /translation="MKFFYADALEHRRHLKLSALFDLCFPSPGLPGALPADADGLWLA
                     ADENDELTGCLLLFQTDLHSWEGIPFVHPAYRRKGIFSALLANACQKGNCPGEDDLFF
                     SVRPTQTLWLQVLNSIEAEKAFDEYMMEYSFQNLPSARLKTEADFSDELTVSFQGTEG
                     MASCPHVSCLLSAHGSSVYLYQLLTEEAFRGQGRALAFLHHLFPRLKALGFSSVRLQV
                     SGENLPAMALYKKTGFHITETLSFYLY"
     gene            3599..3961
                     /locus_tag="ACZPCG_02000"
     CDS             3599..3961
                     /locus_tag="ACZPCG_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HIR1283878.1"
                     /translation="MKYVKQIGIILGVTLAGEALSHFLPFPVPAGVYGLFLMLTALMS
                     GIVKLESVEGTGNFLMDTMSMMFIPATVGIIESADQLREVLLPFLLIIATSTVIVMAV
                     TGTVAQRMVESGNKKEED"
     gene            3966..4670
                     /locus_tag="ACZPCG_02005"
     CDS             3966..4670
                     /locus_tag="ACZPCG_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HIR1283879.1"
                     /translation="MKEILKEFLEQSQYWGFVFSVGAYLSACWLKEKTRLALLNPLLV
                     SAAVIIVFLLGTGVDYAVYSQSASGLGWLLTPATVCLAIPLYKQLHLLKKYGAAVFAS
                     IGSGVAASVVSIFLMCRLMGLSHVNYVTLLPKSITTAIGMGVSEEAGGIVTLTVMSII
                     LTGVIGNVLGELVFRITGVRHPIARGLALGTSAHAIGTAKALELGEIEGAMSSLSIAV
                     AGLMTVIAVPIAANLI"
     gene            4771..7176
                     /locus_tag="ACZPCG_02010"
     CDS             4771..7176
                     /locus_tag="ACZPCG_02010"
                     /inference="COORDINATES: protein motif:HMM:NF024916.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4125 family protein"
                     /protein_id="HIR1283880.1"
                     /translation="MFQLDEFMNGLDRLYENGRMDLAEEYLTEGMKTARQADDKGGIL
                     AVLNELIGYYRVRGEYEKCVLCAKEAMELVRELGLSGTLHHGTTCLNAATGFRAAGRY
                     EEAEAAYREAEKVYASLLKEPDYRLAGLHNNLSLLYAETGRLSEAEKRGLKALDMIRR
                     LPDAQVECATTLTNLGNICFKMHRTARGARYMKEAAALFENLPGNPDPHYPSALAGLA
                     EACFHRGELDEAEAYYRQAMELINRLYGKNDGWQTLNRNREQVLDVKARREAFEKSGI
                     CGLDLARSFYEQKGRPMLLEKYPAYADRIAAGLAGEGSECLGLDDVYSTDHDFGPGFC
                     FWLTKEDYEAIGEQLQADYDRLAESFKGIPVRNTTKEGAGRVGVIEIGAFYKRLTGLE
                     HAPDCKTAEDMAAWNRIPPEQLRTAVSGAIFEDKLGEFTRRRREFEACPEKVRLYRLG
                     LCLGKMAQSGQYNYERGRRRRDIGMMQASLAEFIQASAEAGYLLNRQYMPFYKWRLRA
                     MEAFICVRELKGALEQLAGSRPDEEETEERIENVCGIILDELKKQGLTDSDELFLERQ
                     KAEVLSRMEKLSGGKTQKEEKSSESDAAPSKEIRTLADEITAMEWQQFTQVKNQGGRA
                     SCQEDPYTFDIMRKSQFYTWDLPVLESYQEDLKEAKQSGWNLLTEKYARMMEHTAPEE
                     YDALKDRLPYRDPARKVLEELLVSLMMDWTRETAEKFPSVMSMGRSLSASEDGMFNTS
                     SETYLKGELGTYSDKTLKLYAAMLLKWMKDGRNPVRETLEHTVAFYGYPSLEEAEQKL
                     GRR"
     gene            7197..8033
                     /locus_tag="ACZPCG_02015"
     CDS             7197..8033
                     /locus_tag="ACZPCG_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006773244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR1283881.1"
                     /translation="MAANKLYSIGEVSKLCNISTKALRFYDKIGVIAPDEVGENGYRY
                     YTLDTILRIPILKYYKQMGFRLEEMQGLVKGGDYYVVGSCFRSKINELREQEHAIHNS
                     YVSVKDWYELIQEARAVLYNKIQEVGMRYVSEDHYCYMDQEFEYHYMDSIINIPWVNY
                     LDTVKNEITGPVILEFPSWKEKMKRTCRQARIMQKPIHPCCCGTNEINFGGFLAATIY
                     HVGSHDRIEESYQKLAEWAKAKGASLGPNVYERYVVDYWTTANEDEFVTEIILPIRIL
                     EE"
     gene            8367..8654
                     /gene="eutM"
                     /locus_tag="ACZPCG_02020"
     CDS             8367..8654
                     /gene="eutM"
                     /locus_tag="ACZPCG_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HIR1283882.1"
                     /translation="MKYDALGMIETKGLVASIEAADAMVKAANVTLIGKEKVGGGLIT
                     VLVRGDVGAVKAATDAGAAAAQRIGELVSVHVIPRPHGEVETILPGGGKAE"
     gene            8673..9674
                     /locus_tag="ACZPCG_02025"
     CDS             8673..9674
                     /locus_tag="ACZPCG_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR1283883.1"
                     /translation="MSGNMTNTSAADMKMKQLSKKFRVTGLTAGALSGLTYGIYTTLV
                     LVAGYYEPLVSAVGILAAPYVCSGLNDLFAGIWLTIYNAKKGRLAEIGRTANTFPGKM
                     MILGALLGGPIANGAYLVGLALAGAYAIPISATCGLFGALFAWIFLKQRPTKRVVAGM
                     LTCVAGAIIINWVKPEGSPNFTLGIICALIAAVCWGLEGMLSSYGGAMIDTDVATNIR
                     QLFSGLVDLVIIVPLVGGLGLLGGTMSAGIPALWLAVSGLSAGASFLLWYKANSTVGC
                     AIGMSLNVTYAFWGVLFCILFLGQPVTTTIVVGSVVIIFGAILVTMNPLDFFKKGEV"
     gene            9676..9960
                     /locus_tag="ACZPCG_02030"
     CDS             9676..9960
                     /locus_tag="ACZPCG_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1283884.1"
                     /translation="MLLPARMAVLNYLSTVKNADINEIMEAMKPQYGHEKQFTKDMYL
                     DHTMSLEANGMCTLVSHELDVNGDLSLRFAITDDGRTTVEKYVPAKYRSR"
     gene            9978..10274
                     /locus_tag="ACZPCG_02035"
     CDS             9978..10274
                     /locus_tag="ACZPCG_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HIR1283885.1"
                     /translation="MRYYGEEALGLVETLGLVPALEAADKMLKAADVELISYENVGST
                     LVTIMVKGDVAAVRSSVEAGAAAAAAIGKLTAKNVMPRPIRAVGEIVSVHDIDA"
     gene            10310..10621
                     /locus_tag="ACZPCG_02040"
     CDS             10310..10621
                     /locus_tag="ACZPCG_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006773242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HIR1283886.1"
                     /translation="MERYEAIGCIETFGLVFVLEAADAMCKAADVELIGYENVASGYI
                     SVIVQGDVGACRTAVDAGVKAVENMGAEVYSSVVIPRPHQDLGKIVTRYSLTNLLPQE
                     E"
     gene            10647..12017
                     /locus_tag="ACZPCG_02045"
     CDS             10647..12017
                     /locus_tag="ACZPCG_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007715244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR1283887.1"
                     /translation="MKFIDKDLKAVQEARVLMEEAAEAKKTLALFEQKKLNRIAACMM
                     DALELKLEQLTEEAVRETGYGDAKDQLLQAKLLIKKMRKTLEPMKCVGVLSTDEAQGV
                     LEIGVPMGVIAAAAPAVSPVAAVLSAAVCAVKSGNAIVFAPHPRAVKTTVRTVHILEE
                     AALEAGLVRGALSCSETAAKEGAIELLSHPETNLILNMGERPLLEACSRTGCPVLYGG
                     IAPGPVFIEKTADISRAAACITDSRGFNCGTGAGSEQYVVADRQIAAQVKEELIRHGA
                     YFMTETEQKQLTAFLGLEKGTGLPDKEFIGKTAAWLAQKAGFAVPEHTKVLVSEQSYI
                     TDFNPYAKGLLCPVLAFYIEEDWIHACEKCIELLVGESRGNTLTIHSQDPEVIRQFAL
                     KKPVGRVLINTPAVWGAMGVTTDLFPTVILGSVTSKEGITAENVSPLHMIYRRKAAFG
                     IHGLCS"
     gene            12047..14602
                     /gene="cutC"
                     /locus_tag="ACZPCG_02050"
     CDS             12047..14602
                     /gene="cutC"
                     /locus_tag="ACZPCG_02050"
                     /EC_number="4.3.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0016840 - carbon-nitrogen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042426 - choline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline trimethylamine-lyase"
                     /protein_id="HIR1283888.1"
                     /translation="MDIREFSNKLAEATKNMSPEEKASLMKMFESVSQEIGKEDTKKE
                     TAKDLCEYPSEGTQIPDGMTRRLKLLKENYLKQKPSITTYRARAITKIAAENPGMPKI
                     ELRAKCFRYCCETAPLVIQDNELIVGAPCGAPRAGAFSPDIAWRWMVDEIDTIGTRPQ
                     DPFYISEEDKKIMREELFPFWKGKSVDEYCEDQYREAGVWELSGESFVSDCSYHALNG
                     GGDSNPGYDVILMKKGMLDIQNEAREHLTKLHYENPDDIEKIYFYKSIIETTEGVMIY
                     ARRMSEYAKELADKETDPKRKAELYKISEINAKVPAHKPETFWEAIQAVWTIESLLVV
                     EENQTGMSIGRVDQYMYPFYKADIESGRMNDFQAFELAGCMLIKMSEMMWITSEGGSK
                     FFAGYQPFVNMCVGGVTREGRDATNDLTYLLMDAVRHVKIYQPSLACRIHNKSPRKYL
                     KKIVDVVRSGMGFPACHFDDTHIKMMLAKGVSIEDARDYCLMGCVEPQKAGRLYQWTS
                     TAYTQWPICIELVLNHGVPLWYGKQVCPDMGDLNSFKTYEEFEAAVKEEIKYITKWTS
                     VATVISQRVHRELAPKPLMSIMYEGCMEKGKDVSSGGAMYNFGPGVVWSGLATYADSM
                     AAIKKLVFDDKKYTLEQLNEALKADFNGYEQIRTDCLNAPKYGNDDDYADDIAADLIN
                     FTEHEHRQYKTLYSILSHGTLSISNNTPFGQMTGASANGRHAWLPLSDGISPSQGADF
                     KGPTAIIKSVSKMSNDNMNIGMVHNFKIMAGLLDTPEGEESLITLLRTACMFGNGEMQ
                     FNYLDNKTLIEAQKHPEQYRDLIVRVAGYSAFFVELCKDVQDEIISRTMLTHF"
     gene            14633..15559
                     /gene="cutD"
                     /locus_tag="ACZPCG_02055"
     CDS             14633..15559
                     /gene="cutD"
                     /locus_tag="ACZPCG_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0042426 - choline catabolic process
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline TMA-lyase-activating enzyme"
                     /protein_id="HIR1283889.1"
                     /translation="MERKALIFNVQKYNMYDGPGIRTIVFFKGCPLRCKWCANPEGLE
                     RKIQIMFKKNSCVNCGLCVDACPVGIHEITPEGIHRVRRDIDCTGCGKCKSVCPQAAL
                     EVNGQVKTVSELLEIVEEDAAFYSMSGGGVTLGGGECTAQPEAARELLMACKSQGINT
                     AIETCGHTKPDKLFAIAEYVDLFLFDIKHMDPKRHNELTGIGNELILSNLKELLTRRY
                     NVKVRMPMLKGINDSKEEIDQVIQFLLPFRDDPNFKGVDLLPYHKMGVGKYGQLDKPY
                     PIEGDPSLSQEDLDRIEGWMKAYDFPVTVVRH"
     gene            15591..15926
                     /locus_tag="ACZPCG_02060"
     CDS             15591..15926
                     /locus_tag="ACZPCG_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013659042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HIR1283890.1"
                     /translation="MPAGRVIEESVPGKQVTIAHVIASPVPEIYECLGIEGRGAIGIL
                     TLSPFETAMIAADIAAKASDVEIGFLDRFTGSVVISGDVESVDTALRAVTDTLKRVLG
                     FAAAEITRT"
     gene            15923..16402
                     /locus_tag="ACZPCG_02065"
     CDS             15923..16402
                     /locus_tag="ACZPCG_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006773237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HIR1283891.1"
                     /translation="MRKKRIMIIGPGKSGKTTLAAWLEDSSDIRRVQNMVYRKNTLDT
                     PGAYLESPWMHHHLIAAAQDASCVVMTADAKGKREVYPPGFAKAFRVPVIGVVTRCDL
                     AEGDCKGAVRQLIRAGIQPPFYEISIYDTERMKQLRRILDPYRPDASEPEKSEERRE"
     gene            16405..17547
                     /locus_tag="ACZPCG_02070"
     CDS             16405..17547
                     /locus_tag="ACZPCG_02070"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007715236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0051144 - 1,2-propanediol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-propanol dehydrogenase PduQ"
                     /protein_id="HIR1283892.1"
                     /translation="MGQFLMSTRIYTGEASLEEIEHLGLKRAYIICDPFMEKCGRAGE
                     LAAMLNGAGAETRIFSEVVPDPSIEVVAKAIQGMKEFEPDGIIALGGGSAIDTAKAAG
                     HLYGSMNGEGRPLLAAVPTTSGTGSEVTSFAVISDTKAQAKYALRDQALVPDAAFLDP
                     RLTSSVPPAITADTGMDVLTHGLEAYVSAKADDFTDACAEKAIRLVWEYLERAVLDGS
                     DMEAREHMHNASCLAGIAFSNASLGVCHSMAHALGAHFHIPHGRSNALILPHVIAYNA
                     GLEENGENEALLRYEKIADMLGIGGGTPKAAVHGLIRHIRNLMKRIGSPAYVTDLSVE
                     REAFEEAIPQMAEQALKDNCTATNPRVPEKQELEYLYKKLCKGGIV"
     gene            17544..18248
                     /locus_tag="ACZPCG_02075"
     CDS             17544..18248
                     /locus_tag="ACZPCG_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1283893.1"
                     /translation="MRFITEEDLRQRFRRERFTEYEPEAGTRLTPGARQFLSDRGIRL
                     TETENGVRPGNGQTGFGKKEAGSSLKQNEQPRETEAEERSQGAHPAEELPCWQLELGN
                     LQADFLQAGLDLQREDVLVSKEIFELERNLARLASGEEMAEGERRWEMLCPACTGIGP
                     ENCRKCLADCFEITGFHAQSPNGRVVIRLHVLRSRLRKLERELPDDRKEMAHCIINRL
                     SQMICHGFGGKICQKN"
     gene            18233..19075
                     /gene="eutJ"
                     /locus_tag="ACZPCG_02080"
     CDS             18233..19075
                     /gene="eutJ"
                     /locus_tag="ACZPCG_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HIR1283894.1"
                     /translation="MSEELAFAECDRLVREFEQVVKKPVKKRSSIYYTGVDLGTACVV
                     LAVLDENRRPVAGAYRYADVVRDGMVVDYIGAIRLVREMKEELEEKLGTELIYAAAAI
                     PPGTDALDGGAIKNVVQGAGFEITALLDEPTAANQILKIQNGAVVDIGGGTTGISILE
                     NGKVINVDDEATGGTHFSLVLAGAYKLPFSEAEIYKRDKKNHKEILPVLKPVIEKVAS
                     IISRQIEGYNVQGLYLVGGTCCLSGIEDIIGKKTGIPTYKPENPMFVTPLGIAMSCTD
                     QEME"
     gene            19081..19386
                     /locus_tag="ACZPCG_02085"
     CDS             19081..19386
                     /locus_tag="ACZPCG_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HIR1283895.1"
                     /translation="MEYRIIKSPSRGTLEILARRKGSGNAVKLEDVDAVGLVQGRLIE
                     MVCAADVAEKAVGVTVEDIRGSCPQHMIMLAIFGDTASVEAALEEIRRREKEGAEEW"
     gene            19380..19649
                     /locus_tag="ACZPCG_02090"
     CDS             19380..19649
                     /locus_tag="ACZPCG_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007715232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HIR1283896.1"
                     /translation="MVTARLTGNIWATRKSDLLSGYKLLLAEVIGGTRAGESMVVIDT
                     IGAGIGDRVIVACGSSARRMLGSDEIPADAAVVGIIDEDCAFEDS"
     gene            19676..20314
                     /locus_tag="ACZPCG_02095"
     CDS             19676..20314
                     /locus_tag="ACZPCG_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR1283897.1"
                     /translation="MKQLICAADIEALKQAGKTVCVTGPDVIVTPSAKDAAEALGITF
                     SDKEPEKAACTCDGEGIDSEQIYQLLKALMDKGLLGELLTKCGSRPYQCVTHENGLKV
                     VRGNTVKMDVFDTGDPNATAYFQELVSKEESHISAGFLVIDHSEFAWELTYEEIDYVI
                     EGTLTVTIDGKKYTANAGDVLFVPSGSKVIWGSPDKARVFYATYPANWADLL"
     gene            20327..21073
                     /locus_tag="ACZPCG_02100"
     CDS             20327..21073
                     /locus_tag="ACZPCG_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HIR1283898.1"
                     /translation="MQALGMIETRGTLAAIEAVDVMLKAADVTLVEKTKIGGGLVTVT
                     VTGDVAAVTAAVDAGASAAEHLGEGLLVSRHVIPRPHEELEGFFGPEPEDPGKRNEPK
                     AAAGEAEIVSEDEPEEAAEELETASEDEPETVAAGLSEDTGAEEADAETGEDGTAVQE
                     LDAEVGNTAVQEPDISIPNPVTRAWCDGLYDVSGAEAVMTALKNCHVTQLRKLARSYP
                     ELGMAGRELSRANRSRLLKELRQWYETRQK"
     gene            21109..22740
                     /locus_tag="ACZPCG_02105"
     CDS             21109..22740
                     /locus_tag="ACZPCG_02105"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007715229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HIR1283899.1"
                     /translation="MENFDLDLRSIQEARNLARQGEEAAKKMATYTSEQIDKILKSMV
                     EAAEEHAACLGRMAVEETGFGNAVHKAYKNHAASTLLYEDIRDMKTVGIISEDRARGT
                     MDVAAPAGLIMGIVPSTNPTSTVIYKSIIAIKAGNAIVFSPHPSAAKCTLRAAEVMRD
                     AAVKAGAPEGVISCITTPTMAATNELMHCKEVALIIATGGPGMVKAAYSAGKPAIGVG
                     AGNSPAYIERTADVKAAVDAIIASKTFDNGTICASEQSIICERCNHDEVVAELKRHGA
                     YFMTKEETDKVCRLLFKNGHAMNAKFVGRSPEVIGQAAGISVPAGTTVLIGEQGGVGE
                     GYPLSYEKLTTVLAFYTVENWEEACKLSIDLLQNGIGHTMSIHTNDQELVRKFAMKPA
                     SRILVNTGGSMGGTGISTGLNVAFTLGCGTCGGSSVSENVSPLNLINVKKVAYGLKNC
                     STLLKDDRTFRNPEKAQEVQASCSQNTASSQSFSPADIVASFERRQGGCCCGAAVQES
                     SIDTAEQLKEASVKGFTDDAHLADLVRQLIASMNE"
     gene            22752..23402
                     /locus_tag="ACZPCG_02110"
     CDS             22752..23402
                     /locus_tag="ACZPCG_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HIR1283900.1"
                     /translation="MNQCEELLKLLMEAVQQEGQKKEEPCANGGIPVGVSNRHVHLSQ
                     ADLDRLFGPGYELTKMKDLSQPGQYACKEMVTVCGPKGAIEKVRILGPVRRETQVEIL
                     AGDGFKLGVKAPVRLSGDLDGTPGITLVGPKGSVQTEKGVMVAQRHIHMLPADAERFG
                     VHDGQVVDLEAEGPRGGILRNTAIRVTAQSQLECHLDMEEANAFGLGGTSVVHIHC"
     gene            23487..23771
                     /gene="eutM"
                     /locus_tag="ACZPCG_02115"
     CDS             23487..23771
                     /gene="eutM"
                     /locus_tag="ACZPCG_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HIR1283901.1"
                     /translation="MKYDALGMIETKGLVGSIEAADAMVKAANVTLIGKEFVGGGLVT
                     VMVRGDVGAVKAATDAGAAAAQRVGELVSVHVIPRPHAEVETILPSVKEN"
     gene            complement(23874..25043)
                     /locus_tag="ACZPCG_02120"
     CDS             complement(23874..25043)
                     /locus_tag="ACZPCG_02120"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007708229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0004473 - malate dehydrogenase
                     (decarboxylating) (NADP+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent malic enzyme"
                     /protein_id="HIR1283902.1"
                     /translation="MDINQASLQMHYDLNGKIEVVSRKKIETREDLSLAYTPGVAEPC
                     RVIAKDYEQSFKLTRRSNLVAVITDGTAVLGLGDIGPAAGMPVMEGKCALFKEFADVD
                     AFPLCIDSKDTDTIVQTIHLISKSFGGINLEDIAAPRCFEIERRLKELCDIPVFHDDQ
                     HGTAIVVAAALLNAIKVTGKEMGTLRVVINGAGAAGIAIGKHLINMGFGNVILCDING
                     IICEGDEGLNPGQEEISHISNLDHEHGTLADALKNADAFIGVSRPGLVTKEMVASMNK
                     GIVFAMANPTPEIMPDEALAGGAAVVGTGRSDFPNQINNVLVFPGVFKGALSVRAREI
                     TESMKQRASYAIAGMVSDEELTEDNIIPSPLNKAVADVVAKAVADVAVEEGIARI"
     gene            complement(25091..26455)
                     /gene="fumC"
                     /locus_tag="ACZPCG_02125"
     CDS             complement(25091..26455)
                     /gene="fumC"
                     /locus_tag="ACZPCG_02125"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009219311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HIR1283903.1"
                     /translation="MQYRTEHDSMGEIQVPADRYWGAQTQRSFENFKIGAEKIPMEII
                     RAFAILKKAAAMANHRLGKLDETRLSLISAVCDEILAGKLDGHFPLAVWQTGSGTQSN
                     MNVNEVIANRGNEIAGKKLLHPNDHSNMSQSSNDTFPTAMHIAAAAEIEDRLFPALDL
                     LISTFDRLMKENEGIIKTGRTHLQDATPITFSQEISGWKNMLEKSKEMLALSLKGLRE
                     LALGGTAVGTGLNAPKGFDTEVAKAVSELTGKEFVTAANKFHSLTSKDELVFAHGALK
                     GLAANLMKIANDIRWLSSGPRCGLGEIFIPENEPGSSIMPGKVNPTQCEAVTMVAVQV
                     MANDTAVGIAASQGNFELNVFMPVCIYNFLQSVRLLTDALISFNNNCVSGIRPNREKM
                     DENLRRSLMLVTCLNPHIGYENAAKTAKKAYKENISLKEACVSLGFLTEEEFDQVFKP
                     EAMV"
     gene            26651..26959
                     /locus_tag="ACZPCG_02130"
     CDS             26651..26959
                     /locus_tag="ACZPCG_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003500026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HIR1283904.1"
                     /translation="MNKKIKTEAVDHLFDAILTLKTPDECYKFFEDVCTINELLSLSQ
                     RYEVAKMLREGQTYLEIADRTGASTATISRVNRSLNYGSDGYDMVFGRLHLGEDTDGE
                     "
     gene            complement(27223..27633)
                     /locus_tag="ACZPCG_02135"
                     /pseudo
     CDS             complement(27223..27633)
                     /locus_tag="ACZPCG_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004842833.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            27718..27849
                     /locus_tag="ACZPCG_02140"
     CDS             27718..27849
                     /locus_tag="ACZPCG_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1283905.1"
                     /translation="MGETNAYHFSLEGEFFMHKEQAYVETAPRDIIQFKYAESLQIQ"
     gene            27990..29048
                     /locus_tag="ACZPCG_02145"
     CDS             27990..29048
                     /locus_tag="ACZPCG_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HIR1283906.1"
                     /translation="MKLIKTVDAEGAVLCHDITQIIKGVTKDAVFRKGHIVAKEDIPV
                     LLSVGKDQLYVWEKEEGMLHENEAAEILCSLCKGNHMERSEVKEGKIELTADCDGLLK
                     VDNRGLKAVNGFGQMMIATRHGNFAVKKGDKLAGTRIIPLVIEEEKMERAKESALAAT
                     GGRPILDVMPFVHKKVGIITTGNEVFYGRIKDTFTPVIEKKMAQFDTEIIDHVTWNDD
                     DKKVTASILDMVKKGADVVVCTGGMSVDPDDKTPLAIKNTGARVVSYGAPVLPGAMFL
                     LAYLEVEEEGKSRTVAVMGLPGCVMYAKRTIFDLVLPRVMADDEVTEENLASLGQGGL
                     CLNCPVCTFPNCGFGKGV"
     gene            29054..29542
                     /gene="moaC"
                     /locus_tag="ACZPCG_02150"
     CDS             29054..29542
                     /gene="moaC"
                     /locus_tag="ACZPCG_02150"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004462039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HIR1283907.1"
                     /translation="MDFSHFDEQGNAWMVDVGGKEETKREATARGSIFMSPECLKMVK
                     EGSMKKGDVLGVARVAGIMGAKRTSELIPLCHLLNLTKLTVDFTIHEETNEIEAVCTA
                     RTTGRTGVEMEALTGVNVALLTIYDMCKAVDKSMEMSHIYLEHKSGGKSGEFYNEVRR
                     QR"
     gene            29546..>29841
                     /locus_tag="ACZPCG_02155"
     CDS             29546..>29841
                     /locus_tag="ACZPCG_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016441035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR1283908.1"
                     /translation="MRDQTGREIDYMRISITDRCNLRCKYCMPDGIQCMPRWEILSLE
                     ELEAIAVCAADLGIRKIKVTGGEPLVRRDCPQLVRMLKNVPGIEKVTLTTNGVLL"
BASE COUNT         8114 a         6341 c         8429 g         6957 t
ORIGIN      
        1 tcctttctgc gctatgaagg gcgctgtgcc ttcttaaatg aggaaaatct ctgcgatctt
       61 tatattgaac agggaaagga cagcctgtgc cggacctgca ggcagtatcc ccgccataca
      121 gaggaatttg agggctgccg agagatttct ctgtgtcttt cctgtccggt ggctgcagaa
      181 ctgatccttg gctgtaagga accggtccgc tttctggaaa aggaggatga gcgggaggaa
      241 acctatgagg actttgattt tttcctgtat acaaagctca tagatgccag aagccttatt
      301 tttaaactgc tccaggaccg gaagacgccg gtttccctgc ggctttccat ggtgctggct
      361 ttgggccatg actttcaggg aaggattcgg agaaaccggc tttatgaagc cgacgccctg
      421 tttgccagat atgaacggga ggatcggatc aggtattttg aagaaaagaa aattcaggga
      481 agccggtatg attttcttaa aaagctgttt tcccttttgg gggaaatgga gcccttaaac
      541 ctcaaatggc cttccagaag ggggaaaatc gaaggctctc tttacggaag tggggaagag
      601 gcttataaaa agacctggaa cgtatgttta aaggcctgtc ctgaacttga attatggact
      661 gagcagctga tggtatactt tgtatttacc tacttctgcg gaagcgttta caatgaaaat
      721 ccttatggaa agcttaaaat ggccctggcc tctgtactga tcattcagga tatggcttta
      781 gagcgtttta tggagcaggg aagcctggat gtaaaggcca tggcggatac agcacacacc
      841 tattccagag aggtggagca ttcagacgaa aaccgcctgc tcttggagga gcgtcttaca
      901 aaggatccgc ggtttggatt gagggatttt ctgggggcca tgctgtaagg gattaagcct
      961 gtaaaatgca gaaacaaacg ttcaaaggaa tggacattcc gccgggaaag gattataata
     1021 aaaagagtgt ctgcttctgg cagaaaagga aactgaaatt tggaaggaga atatgatcat
     1081 gagcgaatgt aatcatgact gcggttcctg cagcgcaaac tgtgagagcc gtacaacaga
     1141 taaaacaagc tttttagagg ctctggctcc ggaaagcaag gtgaaaaagg tcatcggcat
     1201 tgtgagcgga aagggcggcg tgggcaaatc ccttgtaacc tccatgctgg ctgtaagcat
     1261 gaaccgcaag ggccatcaca cagccattct ggatgcggat attacaggcc cttcgatccc
     1321 catggccttt ggcgtggcag acgagagacc ggctgtgact ccggatggac gtctgatgat
     1381 tccggcaaaa tccttagagg gcgtggagat catgtctgcc aatctgcttc ttgaaaacgc
     1441 cacagatccc gtgatctgga gaggtcctgt gatcgcaggc gctgtaaagc agttctggac
     1501 ggaaaccttg tggcaggacg tggactatat gtttgtggat atgcctccgg gaactggcga
     1561 cgtgcctctg acggttttcc agtctcttcc ggtagacgga attatcatcg ttacctctcc
     1621 tcaggagctg gtaagcatga tcgtggcaaa ggctgttaat atggcaaaga agatggatat
     1681 ccccattctg ggcgtagtgg agaacatgag ctatctggaa tgtccggact gcaaaaaaca
     1741 catccatgta tttggcgaaa gccatgtgga gcaggcagca caggaaaatg gcttaaaggt
     1801 tctggctcag atcccgatcg atccgaagat cgctcagatg gttgacggcg gacgggtaga
     1861 gtacatcgag atgccatggt ttgaaaaggc tgcagaagca gtggaagctc tgtaggacaa
     1921 tatgaggtgg caaaatcaat gaatacgaat cttagcccca acagcgaact tcaccagtcc
     1981 atcgtccgtg ttccggatgt ggcccaggtt ccggatatct ggctggatca ggcaagacag
     2041 ttccagcagg tgatgatgca ctatacctgc gccatccggg aggtaaaaac gaagctggag
     2101 gtcttaaatg acgagctttc ggtaaagaac cagagaaacc ccattgagat gatcaagtct
     2161 cgtgtgaaaa agccgttgag tattgtggaa aagctgcagc gccggggact ggaggtatct
     2221 ctggaatcga tggttaaaaa cttggatgac gtggcgggag tacggattat ctgctctttt
     2281 gtggatgata tttacgaagt ggcagagatg ctggtgcgcc aggatgatgt aaaggtgatc
     2341 gcggtaaagg attatatcaa aaatccaaag cccaacggct accgcagcta tcacatgatc
     2401 gtggaggttc ccgtattttt tacggattcc aagcgtttta tccgcgtaga ggttcagatc
     2461 cgcaccattg ctatggattt ctgggccagt ctggatcatg agctgaaata caaaaagtca
     2521 tttgtagata cagacgggga gatcagcggg gaattaaaag aatgcgccga tgtgattgcc
     2581 cagacagatg aaaagatgct ggagatccga aagcgcattg aagctcaggg agtagaggtg
     2641 cgccgggatt aggcgcggtg tattccgttt aataaagata aaaggacagg gtttcagtga
     2701 tatgaaaccc tgttttttta tacagggcca tggcaggaag attctctccc gatacctgga
     2761 gacggacaga ggaaaatcca agggctttca gccggggaaa aagatggtgc agaaaagcca
     2821 aagcccgtcc ctgtccgcga aacgcctctt cggtaaggag ctgatacaga tagaccgagg
     2881 agccgtgagc ggagagaagg caggatacat gagggcaaga ggccattcct tctgttccct
     2941 gaaaggaaac ggtaagctcg tctgaaaaat cggcttctgt tttcaggcgg gcagatggaa
     3001 ggttttgaaa tgagtattcc atcatgtatt catcaaaggc tttttcggct tcaatggagt
     3061 tgagaacctg cagccagagt gtctgcgtgg gacggacgga aaagaaaagg tcatcctctc
     3121 cgggacagtt ccccttttgg caggcatttg ccagcagagc ggaaaaaatc cccttcctgc
     3181 ggtaagctgg atgaacgaaa gggattcctt cccaggaatg aagatccgtt tggaacagga
     3241 gcaggcagcc ggtcagttcg tcattttcat cagcagccag ccagaggccg tctgcatctg
     3301 ccggcaaagc gccgggaagg ccgggggacg gaaaacagag atcaaaaaga gcggaaagct
     3361 ttaaatgccg cctatgctcc agtgcgtcag cgtaaaaaaa tttcatagaa ttcagatcct
     3421 ttccggatat gttttctttt attgtaatcg gccattgtcc ggaatgcaag accgggtgga
     3481 ttgtatttcc tttgtatttt atgaattcag accttgcgcc tgaaaatggg tggtgataga
     3541 ataggacggc gtataggatg gttgggagag tacatataaa acagtaggag gaatccatat
     3601 gaagtatgta aaacaaattg gcattatcct gggggtcacc ctggcaggag aggccctcag
     3661 ccactttctt ccgtttccag taccggcagg cgtatacggc ctttttttaa tgctgacagc
     3721 cctgatgagc gggatcgtga agctggaaag cgtagagggg accggaaatt ttctcatgga
     3781 taccatgagc atgatgttta ttccggctac cgtagggatt attgaaagtg cagaccagct
     3841 gcgggaggtg ctcctgccgt ttctgcttat tattgccacg tcaacggtga tcgtcatggc
     3901 agttacggga acagtggccc agagaatggt ggagagcgga aataagaaag aggaggacta
     3961 agaggatgaa ggaaatactg aaggaatttt tggaacagtc acagtattgg ggatttgtat
     4021 tcagtgtcgg agcgtatttg tctgcctgct ggctgaaaga aaagacaagg cttgctcttt
     4081 taaatccgct tctggtgtca gcggctgtta tcattgtttt tcttttggga acaggcgtgg
     4141 attatgcggt ctacagtcaa agtgcatccg gtttgggctg gcttttaaca ccggccacgg
     4201 tgtgcctggc gatccctctt tataagcagc tccatctgct gaaaaaatac ggagcggctg
     4261 tatttgcaag cattggatca ggagtggcag ccagcgtggt gagcattttt ctcatgtgcc
     4321 ggctgatggg gctttcccat gtaaattatg tcacgctgct tccaaagtcc attaccacgg
     4381 ctatcggaat gggagtcagt gaggaggcgg gggggatcgt caccctgact gttatgagta
     4441 ttatcctcac aggtgtgatc ggaaatgtac tgggagaact ggtatttcgc atcacggggg
     4501 tgcgccaccc cattgccagg gggctggctt taggcaccag cgctcatgcc attggtaccg
     4561 ctaaggcgct ggagttgggg gagattgaag gggctatgag cagtctctcc atcgctgtgg
     4621 caggtttgat gaccgtgatc gcagttccga ttgcagcaaa tctgatatag ggttggtctt
     4681 gcaattctcg tctggtggtg ataaaataaa caaacattcg cacaaaacaa atatatttta
     4741 tacaaatcac acaaaggagg gggctgcggc gtgttccaat tagatgaatt tatgaatggg
     4801 ctggatcgtc tgtatgagaa cggacgtatg gatctggcag aagaatatct tacagaaggc
     4861 atgaaaacag cgaggcaggc agatgacaag gggggcattc tggctgtttt aaatgaactg
     4921 ataggatatt atcgtgtaag gggagagtat gaaaaatgtg tgctttgtgc gaaagaggcg
     4981 atggagctgg tgagggaact ggggctttcc ggaacacttc atcacggaac tacctgtttg
     5041 aacgcagcca ccggctttcg tgcggcggga aggtatgagg aggcagaagc ggcttacagg
     5101 gaagccgaga aggtatacgc ctctctttta aaggagccgg attaccgtct ggcgggcctt
     5161 cacaataatc tgagccttct gtatgcggaa accggacggc tttcggaggc agagaaaagg
     5221 ggcttaaagg cgcttgatat gatccgccgt cttcctgacg ctcaggtaga gtgtgctaca
     5281 acgctgacca atctgggaaa tatctgcttt aagatgcacc gcacagccag aggcgcacgg
     5341 tatatgaagg aggcggcagc gctttttgaa aaccttcccg gaaatccgga tccccattac
     5401 ccttctgctc tggctggtct ggcggaagcc tgttttcatc ggggcgaact ggatgaggcg
     5461 gaagcgtatt acagacaggc tatggagctc atcaaccgtc tgtacggaaa aaatgacgga
     5521 tggcagacgt taaaccgcaa ccgggagcag gttcttgatg tgaaagcccg aagagaggcc
     5581 tttgaaaaga gcgggatctg cggactggat ctggcaagga gcttttatga acagaagggc
     5641 cgtcctatgc tgctggaaaa atatcctgcc tacgcggacc ggattgccgc aggcctggcg
     5701 ggagaaggct cagaatgtct gggattggat gatgtatatt ctacagatca cgattttggt
     5761 cccggcttct gtttctggct tacaaaagag gactatgaag caatcggaga acagcttcag
     5821 gcagattatg acaggctggc agagagtttt aaggggattc ctgtgcgaaa tacgacaaaa
     5881 gagggagcgg gccgcgtggg agtgatcgaa atcggtgctt tttacaaaag gcttaccggt
     5941 ctggaacatg ctccagactg caaaacggct gaggacatgg ccgcatggaa caggatccct
     6001 ccggagcagt taagaactgc ggtcagcgga gctatttttg aagataaact gggagaattt
     6061 accagacgca ggagggaatt tgaagcctgt ccggaaaagg tacgcctgta ccgtctggga
     6121 ctttgtctgg gaaaaatggc ccagtccgga cagtataatt atgagcgggg ccgccgcaga
     6181 agggatatcg ggatgatgca ggccagcctt gcagagttta ttcaggcttc tgcagaggcg
     6241 ggatatcttt taaaccgcca gtatatgccc ttttataagt ggagattgcg ggctatggag
     6301 gcgtttatct gtgtgcggga gcttaaggga gcgctggaac agctggcagg cagccgtccg
     6361 gacgaggagg aaacagagga gcggattgag aatgtctgcg ggattatttt agatgaactg
     6421 aagaagcagg ggctgacaga cagtgacgag ctgtttttag agcgccagaa ggcggaagtt
     6481 ttatcgcgga tggaaaaact ttctgggggg aaaacacaaa aagaggagaa atcttctgaa
     6541 tctgatgctg ctccgtcaaa ggaaatcagg actctggcag atgagattac cgctatggag
     6601 tggcagcagt ttacacaggt gaaaaatcag ggcggacggg cttcctgtca ggaagatcct
     6661 tacacttttg acattatgcg caaaagccag ttttatacct gggatctgcc ggtgttggaa
     6721 agttatcagg aagatttaaa agaggccaaa caatcaggct ggaacctttt gacggaaaag
     6781 tatgccagga tgatggagca tactgcgccg gaggaatatg atgcattaaa ggaccgtctg
     6841 ccttacaggg atccggcaag aaaagtcctg gaggagctgc tcgtttccct tatgatggac
     6901 tggacaaggg aaacagcaga aaaattcccc tcagttatgt ctatggggcg cagcctttct
     6961 gcatcagagg acgggatgtt caatacttca agtgaaacgt atctaaaagg cgagcttggt
     7021 acatattccg acaaaacctt aaagctttat gcagccatgc ttttaaagtg gatgaaggat
     7081 gggcgcaatc cggtccggga aacgctggag catacagtgg ctttttacgg atatccttcc
     7141 cttgaggaag cggagcagaa gcttggcagg agataggaaa aggaggtata ggggttatgg
     7201 cagcaaacaa actgtattcc atcggagaag taagcaagct gtgcaatatc tctacaaagg
     7261 ctctccgctt ttatgataag atcggcgtga tcgcgccgga tgaggtaggt gagaacggtt
     7321 accgttatta caccctggat accattctcc ggatccctat tttaaaatat tacaagcaga
     7381 tggggtttcg gctggaggag atgcaggggc tggtaaaggg cggagattac tatgtggtag
     7441 gctcctgctt tcgcagcaag atcaatgagc tgcgggagca ggaacatgcg atccacaaca
     7501 gctatgtatc tgtaaaggac tggtatgaac tgatccagga ggcaagggca gtgctttaca
     7561 ataaaatcca ggaagtggga atgcgctatg taagtgagga tcattactgt tatatggatc
     7621 aggagtttga atatcactac atggattcga ttatcaatat tccctgggtc aattatctgg
     7681 acacggtaaa aaatgagatc accgggccgg taatcctgga atttccatcc tggaaggaaa
     7741 agatgaaacg aacctgcaga caggccagga tcatgcagaa acccatccat ccctgctgct
     7801 gcggaacgaa tgagatcaat ttcggcggct ttctggcagc gacgatctat catgtgggct
     7861 cccatgaccg gatcgaggaa agctatcaga agctggcgga atgggcgaaa gcaaaggggg
     7921 catccctggg gccgaatgtt tatgaacggt atgtggtaga ttactggacc actgcaaatg
     7981 aggatgaatt tgtgacagag attattctgc ccatacgaat tttagaggaa taaaacaaaa
     8041 accttcccca taggggaaaa gaatggaaag agctttcact tacaggaaaa cttctttcag
     8101 ggataaaaag cagaaggaaa acccttctgc ttttttgtgt acgccggttt cttcgtcata
     8161 ttgctgtgaa aaaagtaacg atattatgcg atatatacaa taaaagatgg agaaatcgtc
     8221 tgttgaaaaa tcgataaatt acacttgacc ttccacatat gggaatggat tatactaaat
     8281 gtacagaaaa tgtatgcttt ttgtgaaaag atcatgcata ctgcgcgcgc tggctgtaaa
     8341 aaagaagaaa aagaaggaga agaaacatga agtacgatgc attaggaatg atcgagacaa
     8401 agggacttgt agcatctatt gaagcagcag atgccatggt aaaggctgcc aatgtaactc
     8461 tgatcggcaa ggaaaaagta ggaggcggcc tgatcacagt tctggtaaga ggcgatgtag
     8521 gcgcggttaa ggcagctaca gatgcaggcg ctgctgcagc ccagaggatc ggagagctgg
     8581 tatccgttca tgtaatccca cgtccccacg gagaggtgga gaccatcctt ccgggaggcg
     8641 gaaaggcaga ataatcaaag gagagaaacg caatgagcgg caatatgaca aacacatctg
     8701 ctgcggatat gaagatgaag cagctgagca aaaaattccg cgtaacaggc cttacagcag
     8761 gagctctttc cggccttaca tacggaatct atacgacttt ggtattggtt gcgggctatt
     8821 atgaaccact tgtaagtgcg gttggtatcc tggcagcccc ttatgtatgt tccggactga
     8881 acgatctgtt cgccggtatc tggcttacga tctacaatgc aaaaaaggga cgtctggcag
     8941 agattggacg tacagccaat accttcccag gaaagatgat gatcctggga gcacttttgg
     9001 gcggccctat cgctaacggc gcttatctgg taggcctggc tctggccggt gcttatgcga
     9061 tccccatttc tgcaacctgc ggcctgttcg gcgctctgtt tgcatggatt ttcttaaagc
     9121 agcgtccaac caagcgtgtg gtagcaggta tgcttacctg tgtggcaggc gctatcatca
     9181 tcaactgggt aaaaccggaa ggcagcccga actttacact gggaattatc tgcgcactga
     9241 tcgcggctgt gtgctgggga cttgagggaa tgctttccag ctacggcgga gccatgatcg
     9301 acacagatgt ggcaaccaat atccgtcagc tcttctccgg tctggtagat ctggtgatca
     9361 tcgttccctt agtaggcgga cttggacttt taggcggcac catgtcagct ggtattccgg
     9421 ctctgtggct ggcagtatcc ggactgagcg caggcgcttc cttccttctg tggtacaagg
     9481 caaattccac agtcggctgt gccatcggca tgagcttaaa cgttacatat gcattctggg
     9541 gcgtattatt ctgtatcctg ttcctgggac agcctgtaac aactaccatc gtagttggtt
     9601 ctgttgtaat catctttggt gcgatcttgg ttaccatgaa cccgctggac tttttcaaga
     9661 agggagaggt gtaagatgct gctgcccgca agaatggcgg tgctcaacta tttaagcact
     9721 gtgaaaaatg ctgatatcaa cgagatcatg gaggcaatga agccccagta cggacatgag
     9781 aaacagttta caaaggatat gtatctggat catacgatgt ccttagaggc caacggaatg
     9841 tgcactctgg taagccatga gctggatgta aacggagatc tttctctccg atttgccatt
     9901 acagacgacg gccgcacaac cgtggaaaaa tacgttcctg ctaaataccg cagcagatag
     9961 gaaaggagaa acggcatatg agatattatg gtgaagaggc tctcggactg gtagagaccc
    10021 ttggactggt tccggctctg gaagcggcag acaagatgtt aaaggccgca gatgtggagc
    10081 tcatctctta tgagaatgtt ggatcgactc tggtaacaat catggtaaag ggcgatgtgg
    10141 ccgctgtgcg ttcctctgtc gaggccggcg ccgcagccgc agccgcgatc ggaaagctga
    10201 cagcaaagaa tgtaatgcca agaccgatcc gtgccgttgg agagatcgtt tcggtacacg
    10261 atattgacgc gtgaccgctg ctataaaccg agatgtgaga gggaaaaata tggaaagata
    10321 tgaagccatt ggatgtattg agaccttcgg cctggtattt gttctggagg ctgcagacgc
    10381 gatgtgcaag gcggctgatg tagaactgat cggctatgaa aatgttgctt ccggttatat
    10441 ttccgttatt gtacagggcg atgtgggagc ctgccgtaca gcggtagatg ccggggtgaa
    10501 ggcagtagaa aatatgggag cagaggtata cagctctgtg gtgatcccaa gaccgcatca
    10561 ggatctgggc aagatcgtta cccgctacag tctgaccaat cttttgcctc aggaagagta
    10621 aataagaggc agaagggagc agtgaaatga agtttatcga caaagactta aaggcagtcc
    10681 aggaagcaag agttttgatg gaagaggcgg ccgaagcaaa gaaaacactg gctctttttg
    10741 aacagaagaa actgaaccgg atcgcagcct gcatgatgga tgctttagag cttaagctgg
    10801 aacagctcac agaagaggcg gttcgggaaa cgggatacgg agacgcaaag gatcagctgc
    10861 ttcaggcaaa gctcctgatc aagaaaatgc ggaaaactct ggaacctatg aaatgcgtag
    10921 gcgttctcag cacagatgag gctcagggcg ttttggaaat cggcgttcca atgggcgtga
    10981 tcgctgcggc tgcaccggct gtaagcccgg tggctgcggt tctgagcgct gccgtatgcg
    11041 cggtaaaatc aggaaacgcc attgtttttg caccccatcc cagagcggtg aagaccacgg
    11101 tgcgtaccgt acatatcctg gaggaagcgg ctcttgaggc cggactggtc cggggagctt
    11161 tatcctgcag tgagacagcg gcaaaggaag gggccatcga gcttctttca cacccggaga
    11221 caaatctgat cctgaatatg ggtgaacggc cgcttttgga agcctgcagc cgcaccggat
    11281 gcccggtttt atacggaggg atcgctcccg gaccggtatt tattgaaaaa acagcagata
    11341 taagcagggc ggcggcatgt attacggaca gccggggctt taactgcgga accggagcag
    11401 gaagcgagca gtatgtggtg gcagaccggc agatcgccgc tcaggtaaag gaggaactga
    11461 tccgtcacgg ggcctatttt atgacggaga cagaacagaa acagctgact gcgtttctgg
    11521 gactggaaaa aggaacgggg cttccggata aggagttcat cggaaaaacg gcagcatggc
    11581 tggctcagaa agccggattt gcagtgccgg agcatacaaa ggtccttgta tccgagcagt
    11641 cttatataac ggattttaat ccatatgcca aagggctttt atgcccggta ctggcatttt
    11701 acattgaaga agactggatt catgcctgtg aaaaatgtat tgagcttctg gtgggagaaa
    11761 gccggggcaa tacgcttacc attcattcgc aggatccgga agtcattcgg cagtttgcct
    11821 taaagaagcc cgtaggccgt gtgctgatca atacgccggc tgtatggggc gccatgggag
    11881 ttacaacaga cctgttcccc acagtgatcc ttggaagcgt gacctcaaaa gaggggatca
    11941 ctgcagagaa tgtatcgcct cttcacatga tctatagaag aaaggcagca tttgggatcc
    12001 atggactctg ttcataaaca ggcagccaga aagagaggga tttattttgg atattagaga
    12061 attttcaaat aagcttgcag aagcgacaaa aaatatgtct cctgaagaga aagcttctct
    12121 gatgaagatg tttgaatctg tttcacagga aattggaaaa gaagatacaa aaaaagagac
    12181 cgcaaaggat ctgtgcgagt atccttctga gggaactcag attccggacg gcatgacaag
    12241 acgtttaaag ctgttaaagg agaattactt aaagcagaag ccatccatca cgacctaccg
    12301 tgcaagagcg atcacaaaga ttgctgctga aaatcccggt atgccaaaga tcgaattaag
    12361 agcaaagtgc ttccgctact gctgtgagac agctcctctg gttattcagg ataacgagct
    12421 gatcgtaggc gcaccctgcg gcgcacccag agccggcgca ttctcaccag atatcgcatg
    12481 gagatggatg gtagatgaga ttgataccat cggaacaaga cctcaggatc cattctacat
    12541 ttccgaggaa gacaagaaga tcatgcgtga ggagctgttc ccattctgga agggcaaatc
    12601 cgtagatgag tactgcgagg atcagtaccg tgaggcaggc gtatgggagt tatccggcga
    12661 gtccttcgta tccgactgct cctaccatgc attaaacggc ggcggcgact ccaaccccgg
    12721 ctatgatgta atcctgatga agaagggtat gctggatatc cagaatgaag caagagagca
    12781 tttaacaaag cttcactacg agaatccaga tgatattgag aagatatact tctacaagtc
    12841 cattatcgag acaactgaag gtgtaatgat ctatgccaga agaatgtccg aatatgcaaa
    12901 ggaactggct gacaaagaga ccgatccaaa gagaaaggca gagctttaca agatctccga
    12961 gattaatgca aaggttcctg ctcacaagcc tgagaccttc tgggaggcaa tccaggcagt
    13021 atggaccatc gagtccctgc ttgttgtgga agagaatcag accggtatgt ccatcggccg
    13081 tgtagaccag tatatgtacc cgttctacaa ggcagacatt gagtccggca gaatgaatga
    13141 cttccaggca tttgagcttg caggctgtat gctgatcaag atgtctgaga tgatgtggat
    13201 caccagcgag ggcggttcca agttctttgc aggctatcag ccattcgtaa atatgtgcgt
    13261 aggcggcgtt acaagagagg gccgtgatgc aaccaatgac ctgacctatc tgttaatgga
    13321 tgcagtacgt catgtaaaga tttaccagcc gtctttagcc tgccgtatcc acaacaagtc
    13381 cccgagaaag tacttaaaga agatcgtaga tgtagtacgt tccggaatgg gcttccctgc
    13441 ctgccacttt gatgatacac atatcaagat gatgctggca aagggcgttt ccatcgagga
    13501 tgcaagagat tactgcctga tgggctgtgt agagcctcag aaggccggcc gtctctatca
    13561 gtggacctct accgcttata cacagtggcc catttgtatc gagcttgtat taaatcacgg
    13621 tgttccgctg tggtacggca agcaggtttg cccggatatg ggtgacttaa acagctttaa
    13681 gacatatgaa gagtttgaag ctgccgttaa ggaagagatc aaatacatca caaagtggac
    13741 cagcgttgct acggttattt cccagagagt tcacagagag ctggctccga agcctctgat
    13801 gtccattatg tacgagggct gtatggaaaa gggaaaggac gtatcctccg gcggcgctat
    13861 gtataacttt ggaccaggcg ttgtatggtc cggacttgct acctatgcag actccatggc
    13921 agccatcaag aagctggtat ttgacgataa gaaatatacg ctggagcagt taaatgaagc
    13981 attaaaagca gactttaatg gatatgagca gatccgcacc gactgtctga acgctcctaa
    14041 gtacggaaat gatgacgatt acgcagatga tattgcggca gatctgatca actttaccga
    14101 gcatgagcac agacagtaca agactcttta ctctatcctg agccatggta ctctgtccat
    14161 ttccaataat accccattcg gccagatgac cggtgcgtct gcaaacggac gccatgcatg
    14221 gctgcctctg tctgacggaa tcagcccaag ccagggcgct gacttcaagg gcccgaccgc
    14281 tattatcaag tctgtatcca agatgtccaa tgataatatg aacatcggta tggtacataa
    14341 cttcaagatc atggcaggcc ttcttgatac tcctgagggc gaggaaagcc tgatcaccct
    14401 gctgcgtaca gcctgcatgt ttggaaacgg cgagatgcag tttaactatc tggataacaa
    14461 gaccctgatc gaggctcaga agcatccaga gcagtaccgc gacctgatcg tgcgtgttgc
    14521 cggatacagt gctttcttcg tagagctgtg caaggatgta caggatgaga tcatcagccg
    14581 taccatgctg actcattttt aatccgggcg tggggcagtt aggagagata tcatggaaag
    14641 aaaagctctg atctttaatg ttcagaaata caatatgtat gatggaccgg gaatccgcac
    14701 catcgtattc ttcaaaggct gtccgctgcg ctgcaaatgg tgcgccaacc cggaaggtct
    14761 ggagcggaag atccagatta tgtttaagaa aaactcctgc gtcaactgcg gcctttgtgt
    14821 ggacgcctgt ccggtgggga tccatgagat cacaccggag ggcatccacc gggtgcgccg
    14881 ggatattgac tgtaccggct gcggaaaatg taaatccgta tgccctcagg ctgctctgga
    14941 agtcaacggc caggtaaaaa ctgtatccga gcttcttgag atagtagagg aggacgctgc
    15001 attttacagt atgtcaggcg gcggcgttac cctgggcggc ggcgaatgta cggcacagcc
    15061 ggaagctgcc agggagctcc ttatggcctg caagagccag ggaatcaata cggccattga
    15121 aacctgcgga cacacgaagc cggacaagct ctttgccatt gcggaatatg tggatctgtt
    15181 cctgtttgat attaagcata tggatcccaa acgtcataat gagctgacag gaattggaaa
    15241 tgaattgatc ttatccaatt taaaagagct tttgacaagg cgctataacg tgaaggtgcg
    15301 tatgccgatg ttaaagggta tcaatgacag caaggaagaa attgatcagg tgatccagtt
    15361 tctgcttccg ttccgggatg atccaaactt taagggagtg gatcttcttc cttatcataa
    15421 aatgggcgtg ggaaaatacg ggcagctgga taagccctac cccattgagg gagaccccag
    15481 cttaagccag gaggatttag accggatcga gggctggatg aaagcctatg atttccctgt
    15541 aaccgtagtg cgccactgac aggtaaggag gagccgtgac agaaggaatt atgcctgccg
    15601 gacgggtgat tgaagaatcg gttccgggca aacaggtgac gatcgcccat gtaatcgctt
    15661 ctccggtgcc ggagatctat gaatgtcttg gaattgaggg cagaggagcc attggcattc
    15721 tgaccctttc tccctttgag acagccatga tcgcggcgga tattgcagcc aaggcgtctg
    15781 atgtggagat cggattttta gaccgcttta ccggttctgt agtgatttcc ggagatgtgg
    15841 aaagcgtaga tacggccctc agggctgtga cggatacgct gaaacgggtg ctgggatttg
    15901 cggccgcgga gataacgaga acgtgaggaa gaaacggatc atgatcatag ggcccggaaa
    15961 aagcggaaaa acaacgctgg ctgcctggct ggaagacagc agcgatatcc gcagggtcca
    16021 gaatatggtt tacagaaaga atacactgga tacgccggga gcgtatctgg aaagcccatg
    16081 gatgcaccat catctcattg cggcggctca ggacgcctcc tgtgtggtga tgactgcgga
    16141 tgcaaaggga aaacgggagg tatatcctcc gggatttgca aaagccttcc gcgttcctgt
    16201 catcggagtg gtcacacgct gcgacctcgc agagggagac tgcaaaggag cggtacgcca
    16261 gctgataaga gccgggatcc agccgccctt ttatgagatc agcatctatg atacagaacg
    16321 tatgaagcag cttcggagaa ttctggatcc atacaggccg gatgcttcgg agccggaaaa
    16381 atcagaagaa aggagggaat gatgatggga cagtttttaa tgagtacccg gatctataca
    16441 ggggaagctt ctctggaaga aattgaacat ctgggactta aaagagcata tattatctgc
    16501 gatcctttta tggagaagtg cggacgggcc ggagagcttg ctgccatgct gaatggggct
    16561 ggagcagaaa caaggatttt ctcagaggtc gtaccggatc catccatcga ggtggttgca
    16621 aaggccattc aggggatgaa ggaatttgaa cccgatggga tcattgctct gggaggcggt
    16681 tcagccattg ataccgccaa ggcagccggt catctttatg gctccatgaa cggagaggga
    16741 aggcctcttc tggcagccgt tcccaccacc agcggaacag gaagtgaggt aacatccttt
    16801 gcggtgattt cggatacaaa ggctcaggca aaatatgcgc tgcgggatca ggctctggta
    16861 ccggatgcag cgtttttaga cccaaggctg acatcttccg ttccgccggc gattacggca
    16921 gatacaggaa tggatgtgct gacccatgga ctggaagctt atgtttccgc aaaggcggat
    16981 gattttacag atgcctgtgc agaaaaagcg atccggctgg tatgggaata tctggaacgg
    17041 gccgtgttgg acggttctga tatggaagcc agagagcata tgcataatgc ctcctgtctg
    17101 gcaggaatcg cattcagcaa tgcttcctta ggcgtctgcc acagtatggc tcatgccctg
    17161 ggagcacatt tccatattcc tcacggcaga agcaatgctt taatccttcc tcatgtgatc
    17221 gcatataatg cgggactgga ggagaatgga gaaaatgaag cactgctccg gtatgaaaag
    17281 attgcggata tgctgggaat cggaggcggt actcccaaag cggcagtaca cggcctgatc
    17341 cgccacatcc ggaatctgat gaagcggatt ggaagtcctg cttatgtgac agacctgtcc
    17401 gttgaacggg aggcatttga ggaagccatt ccccagatgg cagagcaggc gttaaaggat
    17461 aactgtacgg ctaccaatcc cagagtgcct gaaaagcagg agctggaata cctttacaag
    17521 aagctttgta aagggggaat cgtatgagat ttatcacaga agaggatttg aggcagcggt
    17581 ttcgcagaga acggtttaca gaatatgagc cagaagcggg aacgcggctt acaccgggag
    17641 caagacagtt tttaagtgac cgcggtatcc gcctgacaga gacagaaaat ggcgttcgtc
    17701 caggaaacgg acagaccggc tttggaaaaa aagaagccgg cagcagtctg aaacagaatg
    17761 aacagccaag ggaaacggaa gcagaggagc gttcacaggg agctcatccg gcagaagaac
    17821 tgccctgctg gcagcttgag cttggaaacc ttcaggctga ttttcttcag gcaggactgg
    17881 atcttcaaag agaggatgtt cttgtttcaa aggaaatctt tgaactggaa cggaatcttg
    17941 ccaggcttgc gtccggggaa gagatggccg aaggagaaag gcgctgggaa atgctctgtc
    18001 cggcctgtac gggaatcggt cctgaaaact gcagaaagtg tttggcagac tgctttgaaa
    18061 tcacaggatt tcatgcccag tcacccaatg gccgggttgt gatccggctt catgtgctca
    18121 gaagcagatt aagaaagctg gaacgggagc ttcccgacga tagaaaggaa atggcacact
    18181 gtatcatcaa ccgattatca cagatgatct gccatgggtt tggaggaaaa atatgtcaga
    18241 agaattagca tttgcagaat gtgaccgcct ggtgagggaa tttgaacagg tggtaaaaaa
    18301 gccggtaaag aagcgctctt ccatttatta taccggcgtg gatctgggaa cagcctgtgt
    18361 ggttttagct gttctggatg agaaccgccg gccggtagca ggcgcttacc ggtatgcaga
    18421 tgtggttcgt gacggcatgg tagtagatta tatcggtgcc atccgtctgg tgcgtgaaat
    18481 gaaggaagag ctggaggaaa agctgggtac ggaactgatc tatgcagccg cagccattcc
    18541 tccgggaacc gacgctctgg acgggggagc aatcaaaaat gtagtgcagg gcgctggatt
    18601 tgaaatcaca gccctgttag atgaacccac agccgccaat cagatcttaa agatccaaaa
    18661 cggagctgta gtagatatcg gaggcggaac cacaggcatt tccattttag aaaatggaaa
    18721 agtgatcaat gtagatgatg aggccaccgg aggaacccat ttttctctgg tattagccgg
    18781 agcttataag cttcccttct cagaagcgga gatttataag agggataaga agaatcacaa
    18841 agaaatcctt ccggtgttga agccggtgat tgaaaaagtg gcttccatca tcagccgcca
    18901 gatagagggt tataatgtac agggactgta tctggtagga ggaacctgct gtctttccgg
    18961 aatcgaggat atcataggaa agaaaacagg gatccctacc tataaaccgg aaaatcccat
    19021 gttcgttacg cctcttggaa tcgctatgag ctgtacggat caggaaatgg agtaaagcat
    19081 atggagtacc ggatcattaa atccccaagc cggggtacat tagagatcct ggcccggaga
    19141 aagggttcgg gaaatgcagt aaagctggag gatgtggatg ccgtaggcct ggttcaggga
    19201 cgtctgatcg aaatggtctg cgccgctgat gtagcagaaa aagcagtggg cgttacggtg
    19261 gaagacatcc ggggcagctg cccccagcat atgatcatgc tggcaatctt tggggatacg
    19321 gcttcggtag aagcggccct ggaagaaatc cgacggagag aaaaggaggg agcggaggaa
    19381 tggtaacagc aagactgaca ggaaatatct gggcaacacg aaaatcagac cttctaagcg
    19441 gatataagct ccttttagct gaggtcatcg gaggcacaag agccggtgag agcatggtag
    19501 tgatcgatac catcggagcc gggatcgggg accgggtgat cgtagcctgc ggatcttctg
    19561 ccagacggat gctgggaagc gacgaaattc cggctgatgc ggcagtagtg ggaattattg
    19621 acgaagactg cgcctttgag gattcataag gcggatcagg agaggatgtg gaaaaatgaa
    19681 acagctgatt tgtgctgcgg atatagaagc actgaagcag gcaggaaaaa cagtatgcgt
    19741 gacaggaccg gatgtgatcg taacaccttc tgctaaggat gctgcagaag cccttggaat
    19801 cacattttca gacaaggagc cggagaaggc agcatgtacc tgcgacggag aaggcattga
    19861 cagcgaacag atctatcagc ttttaaaagc tctgatggac aagggacttt taggagaact
    19921 gctgacaaaa tgcggcagcc gcccctatca gtgcgtaacc catgaaaatg gattaaaggt
    19981 agtccgcgga aatacagtga aaatggatgt atttgacaca ggcgatccaa atgccactgc
    20041 ttatttccag gaactggtaa gcaaagagga atctcatatc agtgcaggat tccttgtgat
    20101 cgatcattct gagtttgcct gggagctgac gtatgaggaa atcgactatg taatcgaggg
    20161 aacgctgacc gttaccattg acggaaagaa atatacggca aatgcaggag atgttctttt
    20221 tgttccatcc ggttccaagg taatctgggg ttctccggac aaggcaagag tattctatgc
    20281 aacctatccg gccaactggg cggaccttct gtaaaggagg acataaatgc aggcgctggg
    20341 tatgatagaa acaaggggaa ccctggcagc cattgaagcg gtagatgtga tgctcaaggc
    20401 agcagatgta acccttgtag aaaagacaaa gatcggcggc ggtctggtga cggttactgt
    20461 aacaggtgat gtggcagccg taactgcagc tgtagatgca ggcgcatcgg cagcagagca
    20521 tctgggtgaa ggacttttgg tcagccgcca tgtgatccca aggcctcatg aagagctgga
    20581 gggatttttc gggccagagc cggaggatcc gggaaaacgg aatgaaccga aggcagcggc
    20641 cggagaggct gaaattgtat ctgaggatga gccggaggaa gcggccgaag agctggaaac
    20701 tgcatctgag gatgagccgg agactgtggc agcagggctg tcggaggata ccggggctga
    20761 agaggctgat gcagagactg gggaagacgg cacagcggta caggagctgg atgcagaagt
    20821 gggcaatacc gccgtacagg agccggacat aagcattccc aatcctgtta cacgggcatg
    20881 gtgcgatggc ctttatgatg tttcaggagc agaggcagtg atgacagctc ttaaaaactg
    20941 tcatgtaacg cagcttcgta agcttgccag aagttatccg gagctgggaa tggccggaag
    21001 ggagctttcc agagcaaacc ggtcccgtct cttaaaggaa ttgagacaat ggtatgaaac
    21061 gcgacagaaa taaaacatcc ggtgtctgac cggagaaagg aactgataat ggagaatttt
    21121 gatttagatc tgcgttccat ccaggaagcc agaaatctgg cccgtcaggg tgaggaagca
    21181 gcaaagaaaa tggccactta tacaagtgag cagattgaca aaattttaaa aagcatggta
    21241 gaagcagcag aagagcatgc ggcctgtctg ggaagaatgg cagtggaaga gaccggcttc
    21301 ggaaatgcag ttcataaggc atataaaaac catgcggcgt ctacattatt atatgaagat
    21361 atccgtgata tgaaaaccgt aggcattatc tccgaggatc gtgccagagg taccatggat
    21421 gtagctgctc cggcaggatt gatcatgggt attgttccat ctaccaatcc tacctctacc
    21481 gtaatttaca aatccatcat tgctattaag gctggaaatg caatcgtatt ttctccccat
    21541 ccatccgcag ccaagtgtac tttaagggca gcagaggtaa tgcgtgacgc agctgttaag
    21601 gcaggcgctc cggagggcgt gatcagctgc attacaacac ctaccatggc ggcaaccaat
    21661 gaattgatgc actgcaaaga ggtggctctt atcatcgcaa ccggcggccc gggaatggtt
    21721 aaggcagcct acagtgcagg aaagccggcc atcggcgtag gcgcaggaaa ctcccctgca
    21781 tacattgaac gtacagcaga cgttaaggcg gcagtagatg ctattattgc cagcaagacc
    21841 tttgacaatg gaaccatctg tgcttctgag cagtcgatca tctgcgagcg ctgtaaccat
    21901 gatgaggtgg ttgcagagtt aaaacgccat ggagcttact ttatgacgaa ggaagagacc
    21961 gacaaggtat gccgtctgct ctttaagaac ggccatgcaa tgaatgccaa gttcgtagga
    22021 cgcagtccgg aggtgatcgg acaggcagcc gggatttctg ttccggcagg aaccacggtt
    22081 ctgatcggag aacagggcgg cgtaggcgag ggatatcctc tttcctatga gaagcttaca
    22141 acagttctgg cattttacac cgtagaaaac tgggaagaag cctgcaagct cagcattgac
    22201 cttttacaga acggtatcgg tcataccatg agcatccata ccaatgatca ggaactggtt
    22261 cgcaagtttg ccatgaagcc ggcttcccgt atccttgtaa ataccggagg cagcatgggc
    22321 ggaaccggaa tcagcacagg actgaatgta gcctttacct taggctgcgg aacctgcggc
    22381 ggaagctctg tttccgaaaa tgtaagcccc ttaaatctga tcaatgtaaa gaaggttgct
    22441 tatggattaa agaactgctc tacccttctt aaagatgaca gaaccttccg caatccggag
    22501 aaggcgcagg aagtacaggc cagctgctct cagaatacag cttcttccca gagctttagc
    22561 ccggctgata tcgtggcatc ctttgaacgc cgccagggcg ggtgctgctg tggggctgct
    22621 gttcaggaat ccagcattga tacggcagaa cagttaaaag aggcctctgt taagggattt
    22681 acagatgacg cccatctggc tgatctggta cgccagctga ttgcatccat gaacgaataa
    22741 ggaggcagaa catgaatcag tgtgaagaac tgcttaagct tttaatggaa gcggtacagc
    22801 aggaagggca gaaaaaggaa gaaccctgtg ccaatggggg gattccggta ggtgtttcca
    22861 accgccatgt ccatctgtct caggctgact tagaccgcct gtttggtccg ggatatgagc
    22921 tgaccaagat gaaggatctt tcccagccgg ggcagtatgc ctgcaaggaa atggttacgg
    22981 tctgcggccc taagggagcg atcgaaaagg tacgcatcct gggtccggta cgccgggaaa
    23041 cacaggtaga aatactggca ggagacggct ttaagctggg agtaaaagct ccggtacggc
    23101 tttccggaga tctggatgga acaccgggaa ttaccctggt gggcccgaaa ggttctgtac
    23161 agacagaaaa aggcgtaatg gttgctcaga ggcatattca tatgcttccg gctgacgcag
    23221 agcgctttgg tgtccatgac ggacaggttg tagatctgga ggcagaaggt cccagaggag
    23281 gtattttaag aaatacggca atccgtgtga ccgcacagtc acagctggaa tgccatctgg
    23341 atatggagga ggcaaatgct ttcggtctgg gaggaacttc cgtggtacat atccattgct
    23401 aaactatatt ggaagtcttc ggctgaaaat ggccggactg taatcatgtg aactaaaaaa
    23461 ttaagataaa tcaggaggaa ataatcatga agtatgatgc attaggaatg atcgagacaa
    23521 agggactggt aggctctatt gaggcagcag atgctatggt aaaggcagca aatgttaccc
    23581 tgattggcaa ggaattcgta ggcggcggcc ttgtaactgt tatggtacgc ggcgatgtag
    23641 gcgcagttaa ggcagctaca gatgcaggcg cagcagcagc tcagagagta ggcgagcttg
    23701 tttccgttca tgtaatccca cgtccacatg cagaggtaga gaccattctt ccttccgtaa
    23761 aggagaacta aaacgtatta aagataagaa aggccgcagc cggatgagtc ctggctgcgg
    23821 cctttttgcg cctgtgtttt gatgttatat ggcttttaaa tagattgtat tctttaaatc
    23881 cgtgcgatcc cttcctctac agccacatca gctaccgctt ttgcaactac gtcggctacc
    23941 gccttgttta acggggaggg aatgatatta tcttctgtaa gctcctcatc agaaaccatg
    24001 ccggcaatgg cataggaggc tctctgcttc atggattcag tgatctctct ggcacggacg
    24061 gagagagcac ctttaaatac gcccggaaat accagtacat tgttgatctg gttggggaag
    24121 tcagagcggc cggttcctac gactgcagcg cctcctgcca aggcctcgtc gggcataatc
    24181 tccggtgtgg ggtttgccat ggcaaagacg atacccttat tcatagaggc aaccatttcc
    24241 tttgtcacca gtccgggacg tgatacgccg ataaaggcgt ccgcattttt taaagcgtcg
    24301 gccagagtgc catgctcgtg atccagattg ctgatatggg aaatttcttc ctggccagga
    24361 tttaagccct cgtcgccttc gcagataata ccattgatat cgcagaggat cacatttcca
    24421 aagcccatat tgatcagatg ttttccgatg gcaatgcctg cggctcctgc tccgttaatt
    24481 accacacgaa gcgttcccat ttcctttcct gttaccttga tggcgtttaa cagggcggct
    24541 gccacaacga tagcagtacc atgctggtca tcatggaata cagggatatc gcacagctcc
    24601 tttaaacggc gctcaatctc aaagcatctg ggagcggcaa tatcctccag attgatgccg
    24661 ccgaagcttt tggaaatcag atgtatggtc tgtacaatgg tatctgtgtc cttggagtca
    24721 atgcagagag ggaatgcatc cacatctgca aattccttaa acagggcgca ttttccttcc
    24781 ataaccggca taccggctgc agggccgata tctcccagac ccaaaacagc cgtaccgtct
    24841 gtgatgacgg ctaccaggtt ggagcggcgg gtcagcttaa aggactgctc gtaatccttt
    24901 gcgatcaccc ggcagggctc ggctacgccc ggagtgtagg ctaaagaaag atcctctctt
    24961 gtttcgattt ttttgcggga aacgacctcg atttttccgt tcaggtcgta atgcatctgc
    25021 agggatgcct gattgatatc catgattttg ttcctcctgt aaaataaaat gtgctctggg
    25081 agcagagtgt ttataccata gcttcgggtt tgaatacctg atcgaattcc tcttccgtga
    25141 gaaagcccag agatacgcag gcttccttca gggaaatatt ttccttatag gctttttttg
    25201 cggttttagc cgcattttca tagccgatat gcggattaag gcaggtcaca agcatcaggg
    25261 aacgacggag attttcatcc atcttttcac ggttgggccg gatgccggat acgcagttat
    25321 tattaaagga aatcagtgcg tcggttaaaa gacggacaga ctggagaaaa ttgtaaatac
    25381 atacgggcat gaatacattt aattcaaagt ttccctgaga ggcagcaatg cctacagccg
    25441 tatcatttgc cataacctga accgccacca tagtgacagc ctcgcactga gtcggattta
    25501 cctttcccgg cataatggag cttccgggct cattttccgg gataaagatt tcacccagtc
    25561 cgcagcgggg gccgctggaa agccagcgga tatcatttgc gatcttcatc agattggctg
    25621 ccaggccttt taaggcgccg tgggcaaata ccagctcgtc cttgctggtg agagagtgga
    25681 acttgttggc tgcggtgaca aattcttttc ctgtgagctc ggaaactgct tttgccactt
    25741 ccgtatcaaa gccttttggg gcgtttaagc cggtgcctac ggcagtgccg cccagagcca
    25801 gctcccgaag tcccttaagg cttaaggcaa gcatttcctt tgatttttcc agcatatttt
    25861 tccagccgct gatctcctgg gaaaaggtga tgggagtggc gtcctgaaga tgggtgcgcc
    25921 cggttttgat gataccctca ttttccttca tcaggcggtc aaaggtggag attaaaagat
    25981 ccagagcagg aaagagcctg tcctcgattt cggccgccgc agcaatgtgc atggcagtgg
    26041 ggaaggtgtc attggaactc tgggacatat tggagtggtc gttggggtgg agcagcttct
    26101 ttccggcgat ttcatttccc cggttggcga tgacctcgtt tacattcata ttagactggg
    26161 tgccgcttcc ggtctgccat acagccagag ggaaatgacc gtcaagcttt cctgccagaa
    26221 tctcatcaca gactgcagat atgagggaaa ggcgtgtttc atccagcttt cccagacggt
    26281 gattggccat ggccgctgcc tttttcagga tcgcaaatgc ccgtattatc tccataggga
    26341 tcttttctgc tccgatttta aagttttcaa agctgcgctg ggtctgagcc ccccaataac
    26401 ggtctgcagg aacctggatt tctcccatgg aatcgtgttc agtacggtac tgcatgattt
    26461 gttagcctcc ttgcgtgtac gtcgtaatat ttttgataac agtataccac acatgaagtt
    26521 gtttgaaaat aatttacttt attttctcga actgttcgca agatatggac tttcaaactt
    26581 taatatgtta aaataactga gtgcagatta tataaataaa aagtcataaa gcaataagga
    26641 gaatatagat atgaataaaa aaattaaaac agaagcagtg gatcatttgt ttgatgcgat
    26701 tcttaccctg aaaacaccgg atgagtgcta taagttcttt gaagacgtat gcaccatcaa
    26761 tgaacttttg tccctttccc agcgctatga ggtggcaaaa atgctcaggg aagggcagac
    26821 ctatctggag attgcggaca ggaccggagc ctctaccgct accatcagcc gtgtaaaccg
    26881 ttctttaaat tacggaagtg acggctatga tatggtgttt gggcgtctgc atctgggaga
    26941 agatacggac ggggagtagg gggtcactta taaagtgggt taaaaacagc acgggctttc
    27001 ttttgtgacg ggcacgtgct gtttcttttg ttggagattg cggaaactgc aggattatcc
    27061 taaattattc acggaacagt atttgaattg ataacagtac catgaatctg aaaattgatc
    27121 catgcagcca taacatcact caattaaccc gtcaaaaggg tataaaatcc ctgtggcaga
    27181 gttcagagca gttgttaagc tgtcaatgtt cgttaatagt tgctattcca gctgtacatt
    27241 cagcttgccg atatttgaaa gtgtctcata gaattcattc ttataggggc aactgctgca
    27301 cagtttgaat gtgatatatc ttgctgaatg aactactttt gcagcaatct tcagcaattt
    27361 taaacggacg gtatcaatgc gttgctttcg catatttgct gataacacca atcttctaaa
    27421 ccaattaaat atgttatacg caagagcatg tacctgcagt ctattggcat ttaccattct
    27481 ggtatgactg cttacggacg caaaatcaaa accggatttg ctttctttaa tgaagttttc
    27541 catcagccct cacttgcagt aaaatttgat gagatactct ggtgaggaat ccatgtttgt
    27601 gacaacaaat gtgtacatgt aaaccatttg gtttatcagg cacttccgat gggggcggtt
    27661 cctgtagggc ataattccat aatagactta aaggatgtgc cctgtgaaga ggttgatatg
    27721 ggagaaacga atgcatatca tttttctctg gaaggagaat tctttatgca taaagagcaa
    27781 gcctatgttg aaactgctcc aagagatatt atacaattta aatatgcgga aagcctgcaa
    27841 atccagtaat ggtaagacac tgcaaatatg aggatcgtgt agaaatacat gatccttttc
    27901 tgccccgcaa accatttaca aattccccca ttcgtggtat catcataaga gcgttatttc
    27961 aaaaaaacaa taacggtgga ggttgcaaaa tgaagctgat aaaaacagta gatgcagagg
    28021 gtgctgtact ctgccatgac attacacaga tcatcaaggg cgtgacaaag gacgccgtat
    28081 tccgcaaggg gcatattgtc gcaaaggagg acataccggt acttttaagt gtgggaaagg
    28141 atcagctgta tgtctgggaa aaggaggagg ggatgcttca tgaaaatgag gcagccgaga
    28201 tcctgtgcag cctgtgcaag ggaaaccata tggagcgctc tgaggtaaaa gagggaaaga
    28261 ttgagcttac ggctgactgt gacggtctct taaaggtgga taacagaggc ttaaaggcag
    28321 tgaacggatt cggacagatg atgatcgcca cccgtcacgg aaattttgcc gtaaagaagg
    28381 gggacaagct ggctggaacc cgtatcatcc ctctggtgat cgaagaggag aagatggagc
    28441 gggccaaaga aagcgcattg gctgccacag ggggacgtcc gatcctggat gtaatgcctt
    28501 ttgtacataa aaaggtagga atcattacca cgggaaatga ggtgttctac gggcggatca
    28561 aggatacctt taccccggtg atcgagaaaa agatggcgca gtttgacacg gagatcattg
    28621 atcatgtgac ctggaatgat gatgataaaa aggtgactgc ttctatcctt gatatggtga
    28681 aaaagggtgc ggatgtagta gtctgcaccg gaggcatgag cgtggatcct gacgataaga
    28741 cgccccttgc catcaaaaat acaggagcca gggtggtatc ctacggggct cctgtgcttc
    28801 cgggagctat gtttttatta gcttatctgg aagtagagga ggaaggaaag agccgtacag
    28861 tggcagtgat ggggcttccg ggctgtgtga tgtatgcaaa gcgtacgatt tttgacctgg
    28921 tgctgccaag ggtgatggca gatgatgagg tgacagagga aaatctggct tctctgggac
    28981 agggcggcct gtgcctgaac tgcccggtct gcacattccc caactgcggt tttggaaaag
    29041 gagtgtagga aaaatggatt tttcacattt tgatgaacag ggaaatgcct ggatggtaga
    29101 tgtaggcgga aaggaagaaa caaaacggga ggccactgca aggggcagta tctttatgag
    29161 cccggagtgc ctgaaaatgg tgaaagaggg ttctatgaaa aaaggcgatg ttctgggcgt
    29221 ggcccgtgtg gccgggatca tgggggccaa gagaacctct gagctgattc ctctgtgcca
    29281 cctgctgaac ctgaccaagc tgactgtgga ctttacgatc catgaggaga caaatgagat
    29341 tgaagcagtc tgtacggccc gtaccaccgg acgcaccggc gtggagatgg aagcgctgac
    29401 cggagtgaat gtggccctcc ttaccatcta cgatatgtgc aaggcagtgg ataagtctat
    29461 ggaaatgagc catatctatt tagagcataa gtccggcgga aagagcgggg aattttataa
    29521 tgaggtaaga aggcagaggt aaaaaatgag ggatcagacg ggaagagaaa tcgattatat
    29581 gcggatctcc attacagacc gctgtaatct gagatgtaaa tactgtatgc cggacgggat
    29641 ccagtgtatg ccccggtggg agatcctttc cctggaagaa ctggaggcga tagccgtttg
    29701 tgcggcggat ctgggaatca ggaagattaa ggttacaggc ggagagccgc tggtgcgcag
    29761 ggactgcccc cagttagtac ggatgctcaa aaatgtcccc ggtattgaga aggtaactct
    29821 caccaccaac ggtgttcttc t
//
LOCUS       DBMDUH010000011        29057 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_1171_length_29057_cov_8.069692, whole genome shotgun sequence.
ACCESSION   DBMDUH010000011 DBMDUH010000000
VERSION     DBMDUH010000011.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 29057)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 29057)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29057
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1171_length_29057_cov_8.069692"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="ACZPCG_02160"
     CDS             <1..514
                     /locus_tag="ACZPCG_02160"
                     /inference="COORDINATES: protein motif:HMM:NF018513.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HIR1283909.1"
                     /translation="MGMTTTSVYIVLSVLVAPALVDFGVEPIAAHMFVFYFGILSCIT
                     PPVATAVFASASQLNVSPIKLGLYTSRIAIPIYIIPFLFVLNPALLMLESAGIWIIAE
                     FILSALTIIALCSSIEGCFMTELKKLERILLFVGAVCELHTSVMVKLFSNLLIVIILL
                     RNIQKKKQRQ"
     gene            627..1019
                     /locus_tag="ACZPCG_02165"
     CDS             627..1019
                     /locus_tag="ACZPCG_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1283910.1"
                     /translation="MRKRLVVLGASVLMAFAMAGMAYGAEWKEDVAGWWYQNDDGSYL
                     VNEWKWVDGNGDGTAESYCFDEQRYLYVNTVTPDGYQVNESGAWVQDGAAQAKTVSMM
                     QSVPSTAENYSNYMAFSHFHSTVVNNLG"
     gene            1109..1282
                     /locus_tag="ACZPCG_02170"
     CDS             1109..1282
                     /locus_tag="ACZPCG_02170"
                     /inference="COORDINATES: protein motif:HMM:NF025645.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4368 domain-containing protein"
                     /protein_id="HIR1283911.1"
                     /translation="MSKVRKYLDLDELISPLLNDMVKAVYIHASDKSKGNREQQIDIS
                     YDLVGILETVFTE"
     gene            complement(1406..2047)
                     /locus_tag="ACZPCG_02175"
     CDS             complement(1406..2047)
                     /locus_tag="ACZPCG_02175"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009253219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HIR1283912.1"
                     /translation="MKIALINENSQAAKNSMICENLKKIVEPMGHEVFNYGMYTAEDE
                     TQLTYVMCGLLSAILLNSKAADYVVTGCGTGEGAMLACNSFPGVLCGHIVDVEDAYMF
                     AQINDGNAIAMPFAKGFGWGAELHLQYVFEKLFGCESGGGYPKERVIPEQRNKKILDE
                     VKKITHTDMMTILKNIDQDFLKTAISGEKFQEYFFANCQVPEIAEYLKSVLNA"
     gene            complement(2150..2416)
                     /locus_tag="ACZPCG_02180"
     CDS             complement(2150..2416)
                     /locus_tag="ACZPCG_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1283913.1"
                     /translation="MFRMWGKLIKDNHLLKDITACRSDYSQSRTQMVFDCLDEICYEF
                     DLEKPIWLDSNIKDFKLHAKTRFGRDHFIESVDFDYLEIQVIEE"
     gene            complement(2483..3190)
                     /locus_tag="ACZPCG_02185"
     CDS             complement(2483..3190)
                     /locus_tag="ACZPCG_02185"
                     /inference="COORDINATES: protein motif:HMM:NF025559.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HIR1283914.1"
                     /translation="MERSTYTVTHHGLLIGLYGKTLEEHLGEYGLDIFANAIMRYAHE
                     RGQRMRKRAVRDGRRLDHETFLSYGELQIPSSPEDYSIGSESPVYTLCCHCCMWQESW
                     ARYGLERYGALYCRYVDLHLIQGFHPDLAMITRSALGLGDSCCTFSYVGYCQTPKSRQ
                     RIAAVKKELAGQYTKDFLYHCGHLVNSFHTCISDILGEETASLITRRTACDFDTYTKD
                     GYSKDILTEAQQDFSEI"
     gene            3374..4483
                     /locus_tag="ACZPCG_02190"
     CDS             3374..4483
                     /locus_tag="ACZPCG_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1283915.1"
                     /translation="MAQAITDYVKFLADARDAVYRVNCDSRTARQLEAEESQKEKELA
                     SLKKEVADTISRTVKARRDEIESGYDREIAKGQDRLKKARAKREKAKDKGVKERIAEE
                     TSQLRDHNRDLKIQLKTMFKKDRVPAFVNTTFFYALYYPRGMKEFGIGFLTFLICFLA
                     FPCGIYFLIPERKWWYLAAVYVADVFLFGGLYLMVGNRIRTHYQDVLKKGREIRNLLR
                     ANHKKIHVITRTIQKDGNEALYDLEKYDDEIACASQELSEIAERKKDAVSNFENVTRT
                     IISDEIEGSRREEMLALEEEIKELSAQLHSLEDSIRDQNIHITDTFGPYLGNEFLEPD
                     RLSELSKLIQSGSAANITEAITLYKAGSQKKADGQ"
     gene            complement(4583..6472)
                     /locus_tag="ACZPCG_02195"
     CDS             complement(4583..6472)
                     /locus_tag="ACZPCG_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1283916.1"
                     /translation="MKILSLHIDGFGKLNDLDLSFEDGLNVLYGKNEAGKSTLHTFIR
                     GMLFGIERQRGRASKNDTYSRFEPWSGTGAYQGWLRLESQGEIFRIERRFQKGNKSLT
                     IINETKGCEETPTAALWERLRCGLSETAYSNTISIGQLKSATDGGMVSELRNYIANLN
                     TSGSIALNITKASALLKSQRREMAAKLVPEAAKRYTALLSEVRTIEHEISDPRYQNRL
                     ASCQADLAQIKEELETHRTEKESLLEKTARGRQILIGSHFTDEASIQNQLRQTQEAYK
                     EYREKTAASGGRVQTAATALCFLAAGILALGMGSCIKKPDMIRGLLTRFGPEHAGTSP
                     AFFTFLTGLCGLGGAVFLAAALMQLWSRRRLKRELSSVTDFLSGILRDHLGDSSISDE
                     SIRRLEQRISELLRLERAVYQSENALKRLTDEAEQLQSREAELSKEIETGQRLQWELE
                     QKLERLSTCRSEAEGLKHALTENERIQEELDALDLAQDTLNRLSSTIRDSFGLYLNKT
                     ASELIAGITGGIYTSMSIDQDLNIFMNTPSHLVPVEQVSSGTMDQIYLAVRLAAAKLV
                     QTEGEQMPLIFDDSFALYDDERLKTALKWLSKTFKGQIILFTCHQREAQLLTANQVDF
                     HYLTL"
     gene            complement(6477..7547)
                     /locus_tag="ACZPCG_02200"
     CDS             complement(6477..7547)
                     /locus_tag="ACZPCG_02200"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR1283917.1"
                     /translation="MKFIHTGDVHWGMTPDADKPWGKERAQAIRDTFKAIIDHAGAVK
                     ADCLFISGDLFHRQPLMRDIKEVNYLFSTIPHVRVVMIAGNHDKIRENSSILSFSWSP
                     NVTFLADPALSSVYFDDINTEVYGFSYHTTEIREPLLEDIQVPLNNRIQILLAHGGDP
                     AHVPMNFNTLSLSPFSYIALGHIHKAQVIAEDKMAYCGSPEPLDLTETGTHGYFEGEI
                     HPVTRRLTRLDFIPAASLRCIPLAVTVTTETTNGGLTDRIINEIDKRGREHIYRFRLK
                     GMRDPDITFDLSSLSARFRIGEIIDESEPLYDFPALFAEHSSDMIGFFIQALQKEDMS
                     PVEKKALYYGVNALLHTTDERS"
     gene            complement(7689..9080)
                     /gene="asnS"
                     /locus_tag="ACZPCG_02205"
     CDS             complement(7689..9080)
                     /gene="asnS"
                     /locus_tag="ACZPCG_02205"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002605622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HIR1283918.1"
                     /translation="MDLITVRELYKHPDQYMDQEITIGGWVRSLRDSKSFGFIVISDG
                     TYFEPLQAVYHDTLENFQQVSRLGVGTAVIVKGTLVATPQAKQPFEIQATEVTVEGAS
                     APDYPLQKKRHSFEYLRTISHLRPRTNTFQAVFRVRSLIAYAIHQYFQERDFVYVHTP
                     LITGSDCEGAGEMFQVTTMDLNNIPKTEDGKVDFKQDFFGKPTNLTVSGQLNGETYAM
                     AFRNIYTFGPTFRAENSNTTRHAAEFWMVEPEIAFADLEDNMNLAEGMLKYIIRFVME
                     HAPEEMNFFNQFVDKGLLDRLNNVLNSEFGRITYTEAVKLLEEHNDQFDYKVSWGCDL
                     QTEHERFLTEQIFKKPVFVTDYPKEIKAFYMKMNPDNKTVAAVDCLVPGIGEIIGGSQ
                     REDDYDKLVARMEELGLKKEDYGFYLDLRKYGSARHAGFGLGFERCVMYLTGMSNIRD
                     VIPFPRTVNNCEL"
     gene            9480..10754
                     /locus_tag="ACZPCG_02210"
     CDS             9480..10754
                     /locus_tag="ACZPCG_02210"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HIR1283919.1"
                     /translation="MIKKEMIAMLLAGGQGSRLGVLTKKVAKPAVSFGGKYRIIDFPL
                     SNCINSGIDTVGVLTQYQPLRLNTHIGIGIPWDLDRNVGGVTILPPYERSKGSDWYTG
                     TANAIYQNLEYMEAYNPEYVLILSGDHIYKMDYEVMLDYHKANNADVTIAAMPVPIEE
                     ASRFGILITDEANRITEFEEKPAVPRSNLASMGIYIFSWPVLRDALIHLKDEPGCDFG
                     KHIIPYCHGKGDRIFAYEYNGYWKDVGTLGSYWEANMELIDIIPEFNLYEEYWKIYTK
                     SDVIPPQYIADDAEIERSIIGEGTEVYGKVINSVIGSGVTIEKGAVVRDSIVMQGTHI
                     GACTVVDKAIIAENVTIGANAEVGFGDYAPSTYDPKVYQFELATIGENSVIPDGVKIG
                     RNTAIAGETAIDDYPDGILAGGNYIIKAGGVK"
     gene            10751..11872
                     /gene="glgD"
                     /locus_tag="ACZPCG_02215"
     CDS             10751..11872
                     /gene="glgD"
                     /locus_tag="ACZPCG_02215"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008720661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HIR1283920.1"
                     /translation="MRAIGIVLAGGNSKRMRELSNKRAVAAMPVAGSYRSIDFALSNM
                     TNSHIQNVAVFTQYNSRSLNLHLSSSKWWDFGRKQGGLFLFTPSITPENGDWYRGTAD
                     ALYQNLTFLKNSHEPYVVIASGDGVYKLDYNKVLEYHIEKKADITVVCKDMEEGMDVT
                     RFGCVKLAADGRIVDFEEKPMASDATTISCGIYVIRRRQLIELLERCAAEDRYDLVND
                     ILVRYKNLKRIYAYKMHSYWSNIATVDSYYKTNMDFLKPEVRNYFFREYPDVYTKIDD
                     LPPAKYNPGAVVRNSLVSSGCILNGTVENSILFKKAYIGNNCVIKNSIILNDVYIGDN
                     TVIENCIVESRDTIRANTTHIGTPDNIKIVVEKNERYTL"
     gene            11950..12231
                     /gene="spoVG"
                     /locus_tag="ACZPCG_02220"
     CDS             11950..12231
                     /gene="spoVG"
                     /locus_tag="ACZPCG_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012198141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HIR1283921.1"
                     /translation="MQVTDVRVRRVEKEGKMKAIVSITMDNEFVIHDIKVIEGEKGLF
                     IAMPSRRSADGEYRDIAHPINSGTREMIQNTILEKYEMTLLENEGAEAE"
     gene            12446..14389
                     /locus_tag="ACZPCG_02225"
     CDS             12446..14389
                     /locus_tag="ACZPCG_02225"
                     /inference="COORDINATES: protein motif:HMM:NF012579.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HIR1283922.1"
                     /translation="MYGGRREEYRMQMDGRLNRLLSVLSEDLYINMETVADQLEVSER
                     TARTLVSQLDELLCRNGAAIERRRGLGLRLCVKEPERYQEFIKGRTSALLPETGKERI
                     EFILARLFADASGVKAEELCERLYISRKTLSLDLKGVEQYLNKHHLALERKPYYGLKI
                     QGDEFSIRLCMSAAFYEFSDQWFKEIYHTFWDGEQIRNEILSSVKQCGETIYEMDIPN
                     IVLQVQIALYRWGRGCVIPLEEMKHSDLLHEGDIRAARLCAEGLKEAFGICLPVPEIK
                     YMAIQLLGKKKILAGDRSSVFIDMEINQLVNRMLESVKQTYNLDLASDFDLNTSLRLH
                     MVSLRIRLQYRLKMDNPILKEIKEVYSFPYAVAAHASTVLSEYFHTIVPEAEIGYLAL
                     CFALSLKRQDRQRYKRNILLVCASGSGSAKLFEYRFREMFGDYLDRVEACDIGSLASW
                     DFSNIDYVFSTVPVKTAIPVPVCQVQYFFDRHNAGEVERLLKSSRKGGIRSYFDRSLF
                     FTNIKGNSREEILHELCGRIRRVRELPPDFEDSVRKRENLMQTDFCPQIAIPHPYQPV
                     TKDTFVCVALLEKPVLWHIYEVQVVFLLSVSVNKENLEEFYRIAPSFMMDEGCMNHLL
                     ETRSYDTLMDIIDWAERDELQNR"
     gene            14429..14752
                     /locus_tag="ACZPCG_02230"
     CDS             14429..14752
                     /locus_tag="ACZPCG_02230"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HIR1283923.1"
                     /translation="MGTIRVLLCCGAGFSSGFLAQKARQAAKKKKLDMTVEARSESVV
                     SEYMDKMDVLLVGPHYASAVPKLTEECAPHGIQVELIPAEIYSSLDGAALVELAEKVA
                     ARKDG"
     gene            14759..15997
                     /locus_tag="ACZPCG_02235"
     CDS             14759..15997
                     /locus_tag="ACZPCG_02235"
                     /inference="COORDINATES: protein motif:HMM:NF014437.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HIR1283924.1"
                     /translation="MNGLMTWLSDVFAPKARQVFANPWIDTVASTMKKVLPIILTGSL
                     IFFYNVFRSYIPALPDLSPIANFSFGLLAIFVAFFVTHEAMIKLGHGQYQINASLISI
                     AAYLLLCRPQVVDGIFQIDNGRIGAAGIMMGIVVGLFVAIVFHIYANLHVLENNDTLP
                     DFVSDWINNIIPITITLGLAMIFANVLKLDLYNILVSFFMPLQKGAQTLPGFILICFV
                     PVIFYSMGISSWVFNAVTTPIFMVAIAENIGAVANGGVPMNIATSEAVFTAALITMGG
                     RGGTLPLNVLMLGSKSKKLKTMGKICIGPSLFNINEPLMFGAPVVFNPLLMLPILINS
                     IVGPTVVWLTMHYGLLNIPSKMVQVGQIPAPFSSVMITGDWRAVPVYIVLFILYLLTW
                     YPFYKVYERQCVEEETQAAC"
     gene            16082..16423
                     /locus_tag="ACZPCG_02240"
     CDS             16082..16423
                     /locus_tag="ACZPCG_02240"
                     /inference="COORDINATES: protein motif:HMM:NF014326.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HIR1283925.1"
                     /translation="MMFKDDDKVVQVAMQIVISAGDARNAAGKALDCVAAFDFAGAKE
                     HMEEANNHICAAHNAQTEMIQSEIAGTETIQPSLLFNHAQDTLMTVMSEIHLSEKMIQ
                     VFESFYNKMNA"
     gene            16434..17879
                     /locus_tag="ACZPCG_02245"
     CDS             16434..17879
                     /locus_tag="ACZPCG_02245"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012457.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HIR1283926.1"
                     /translation="MSADIRKTMPEGFLWGASTSAYQVEGAAEEDGKKLSQMDILNRG
                     TGFADASVASDHYHRYKEDIALMKECGFTAYRFSFSWSRIFPDGVGEVNPKGLEFYDN
                     LVNELVANGIKPIPTIYHYDMPMALVDKYDGWISRQSVDDFVAYGEFLIRRYGDRIKD
                     WLIINEQSIIVEFWKKKNYVPEKYHGDPQVKFQINHHMNLGQAKISKLIHELVEGGRS
                     GAAIGYSPVYGLDSTPKNMLAMLNAEDLKNHFFLDIYFKGKYPVGAFRYLEEHGMAPV
                     IEEGDMEIIREGMLDFLGVNYYASKCVRFPDADKNELTEAKSNLSGKKGAMGAYEVMP
                     DFYQYMKNPNADTNDWDWTIDPMGMQYLLRDIYQRYEIPMIITENGLGARDVLEDGKV
                     HDDYRIDYWTKHLQAIHRAIELGVDVRGFLPWSFVDVLSTSNGYQKRYGLVYVNRDDE
                     DIKDLARIPKDSYYWYQKVIATNGAHCLEEE"
     gene            complement(17937..19340)
                     /locus_tag="ACZPCG_02250"
     CDS             complement(17937..19340)
                     /locus_tag="ACZPCG_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR1283927.1"
                     /translation="MSKNNLYDVIIIGAGPSGIFCAYELIRQKPELKILMVEKGRPIE
                     KRVCPKRTTKVCVGCKPCSITTGFAGAGAFSDGKLSLSPDVGGNLPEILGYDETAALL
                     KESDDIYLKFGADKNVYGIDKQKEIEEIRRKAINANLKLIECPIRHLGTEEGYKIYTR
                     LQEHLLAQGIEMKFNTMVKDILIEGDTVKGIVTDKDETFCAPEIVAAIGREGSDWFSH
                     VCDNHHIETKVGTVDIGVRVEVRDEVMKFLNENLYEAKLVYYTPTFDDKVRTFCSNPS
                     GEVATEYYENGLAVVNGHAYKSQEYKTNNTNFALLVSKNFTRPFNEPIEYGKHIAQLS
                     NMLCGGRIMVQTFGDFQRGRRTTEERLCRNNLIPTLKDAVPGDLSLVFPYRIMTDIKE
                     MILALDKVTPGIASDETLLYGVEVKFYSNKVVVDKDFETSVHGLRAIGDGASVTRGLQ
                     QASANGISVARSILKRM"
     gene            complement(19434..20795)
                     /locus_tag="ACZPCG_02255"
     CDS             complement(19434..20795)
                     /locus_tag="ACZPCG_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009267579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1283928.1"
                     /translation="METVKKNRSGTTDLTVGRPLAQILRFALPLVLGTLFQQLYSFAD
                     TIIVGRCLGTDALGAVGTTYSLNFLILGFIQGACVGFGIPAAESFGAKDHKNLERFLI
                     NGAALCIGLSAVFTVVTTLTAPPLLKLIHTPPELFDDASLYIRIIFLGIPATVLYNYS
                     SSVLRAMGDSRHPFYFLLASSLLNIALDWILIVPFGMGVDGAAIATVISQALSGLLCT
                     WWFFSKTAKGHGLSFTKNKLRLSSHHCKKLAYIGLPMGFEYSVSAIGAVIMQDAINLL
                     GSTAVAAQTAGEKIRQMFTLPMESVGMAMATYAGQNHGAGRTDRIRQGIKDGCKIQLC
                     YCAAAWTAIFLCKDFAVGLVLGDADPAVTAGALEYLSVMSLLFCFHGLLMIFRNTLQG
                     LGYSMQAVISGISELVGRSLGGLLAVTKLGYLGICLANPIAWALAMFYCIFMVTRVMK
                     KER"
     gene            20996..21748
                     /locus_tag="ACZPCG_02260"
     CDS             20996..21748
                     /locus_tag="ACZPCG_02260"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR1283929.1"
                     /translation="MRTGKNWNILKRGLKWTIGIMAAAVICICVNVGMKGYDMYRDAI
                     SQISLKDKVEQIRSKENYTEFAELPETYVDAVLSVEDHRFYGHIGIDPIAITRAVIND
                     IRAGAFVEGGSTITQQLAKNLYFTQEKKMERKAAEVFMAFALEKEYSKNEILELYMNT
                     IYFGNGYYCIKDASEGYFGKAPEDMDDYESTLLAGVPNAPSQYAPTVNPALAAKRQEQ
                     VLERLVECGLFTEKRARETMAETREYQEPDAA"
     gene            21964..22533
                     /gene="pth"
                     /locus_tag="ACZPCG_02265"
     CDS             21964..22533
                     /gene="pth"
                     /locus_tag="ACZPCG_02265"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006771414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HIR1283930.1"
                     /translation="MYLIVGLGNPGREYDKTRHNAGFDTLDVLADMLNTSVEEKKFKG
                     LYGRGLIGGEKVILLKPQTFMNLSGESVREAADFYKIDPEHIIVIYDDISLEPGQLRI
                     RRKGSAGGHNGIKNIIAHLGTQEFPRIKVGVGAKPPRMDLADYVLGRFSAEDRRVMEE
                     AFKTAAKAAQVMITDGAEAAMNQFNGHKE"
     gene            22728..26303
                     /gene="mfd"
                     /locus_tag="ACZPCG_02270"
     CDS             22728..26303
                     /gene="mfd"
                     /locus_tag="ACZPCG_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HIR1283931.1"
                     /translation="MENPLLELQEYENLLEALKKAKGPLQAAGTLESQKVHLMHELGE
                     DGGFPWKLVVTYDEQRAKEIYDDFRSFTRQVWLYPAKDLLFYAADIHGNLMTRQRISV
                     LRHLMEDEGGVVVTTIDGLMDHLMPLAFLKQHAVTVECGQVIELDQWKERLTELGYER
                     TGQVDGMGQFSIRGGIIDIFPLTEEVPVRIELWDDEVDSIRTFDPESQRSVEQLDRVV
                     IYPASETVLTKGQLEKGIKRLEEETASYVKALEGQKRREEAIRIKGIIRELTEGIREG
                     WKRGGLDGYIRYFCPETVSFSDYFPQGDCVVYLDEPGRLKERGETIELEFRESMVHRL
                     EKGYLLPGQTELLYPAGQVLGRLQGPYSIMLTGLDQKLPGMKVNHKFSFDVRNVNSYQ
                     NSFEILIKDLTRWKKEGYRVVLLSASRTRASRLAGDLREYDLRAYCPDGETEGEESRA
                     ERKVLPGEIMVTYGNLHRGFEYPLLKFVFITEGDMFGVEKKKKKRKKQSYQGRAIQSF
                     SELSVGDYVVHEEHGLGIYRGIEKVERDKVVKDYIKIEYGDGGNLYLPATRLESIQKY
                     AGSEAKKPKLNRLGGSEWTKTRAKVKGAVQEIAKDLVKLYAARQEQKGFQYGSDTVWQ
                     KEFEELFPYDETEDQLDAIDAVKTDMESPKIMDRLICGDVGYGKTEIALRAAFKAVQD
                     SKQVVYLVPTTILAQQHYNTFVQRMKDFPVRIEMMSRFCTPAQQKKTLEGLRKGLVDI
                     VIGTHRVLSKDLQFKDLGLLIIDEEQRFGVTHKEKIKQMKENVDVMTLTATPIPRTLH
                     MSLAGIRDMSVLEEPPVDRTPIQTYVMEYNEEMVREAINRELARNGQVYYVYNRVTDI
                     DEVAGKIQAMVPDAVVTFAHGQMKEHELERIMTDFINGEIDVLVSTTIIETGLDIPNA
                     NTMIIHDADHMGLSQLYQLRGRVGRSNRVSYAFLMYKRDKLLKEEAEKRLQAIREFTE
                     LGSGIKIAMRDLEIRGAGNVLGAEQHGHMEAVGYDLYCKMLNQAVLALKGEETEADLY
                     ETSVECDIDAYIPPSYIKNEYQKLDIYKRISTIETDEEYMDMQDELLDRFGDIPKSVE
                     NLLKIAMVRALAHRAYVTEVFVNRQEIRLTMYQKAKLDVTRIPDFVQSYKGELKLVPG
                     ETPVFHYVDRKNKNSDSLIMLEKARTMLEQLGKLGQKEPGGNGAD"
     gene            26330..28279
                     /locus_tag="ACZPCG_02275"
     CDS             26330..28279
                     /locus_tag="ACZPCG_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR1283932.1"
                     /translation="MPDTDYGYALKMGRKRYQDALARGEYPYLPVLDNILANTDIVAT
                     VNLGVMDIPLAKMVGTKTAERSSSFASNFMPLLPERSEFGAKWTSLYKYQVEEGIQEP
                     VVAYEFMNQFYVQEGNKRVSVMKYLGAYSIPGSVTRLIPKRTDDKENRLYYEFLDFYR
                     ISSNCDVWFSKEGSYKKLLELMGMEPDQVWTQEERLFFRSSYGRFAKAYSMAHGDQLQ
                     LTEGDAFLVYIEIYGYDSVKGQTEREMYRALMKIWEEIQLANRGRKVELIEEPEEMET
                     EHKPSIWKWLLPADDMPEHLKIGFVYADTIENSSWNYGHELGRQYLEEAFEGRISTVV
                     IEGADTEGDIARAIEKLVQAGCTMIFTTNSKMVNQSVRSAILHPEVKIYNCSVNMSYS
                     SICTYYARMYEAKFLMGAIAAAVSREDKLGYIADQPTYGILADINAFAMGARMINPYV
                     KVYLEWRRINHEKTAAERLREQGIRYISGKDMIIPRHPSREYGLYVQEDNGEVFNLAS
                     PIWNWGKFYEQMIQLAFESNEELEARKGKKAVNYWWGMSADVIDVICSGNLPHGTLRL
                     IEFLKNSVRSGSFHPFDGFMYDQEGIIRCREGERLRSEDIVTMNWLAENVVGRVPDLE
                     ELNDEARERMQLQGIRVDESVQTEK"
     gene            28284..28889
                     /locus_tag="ACZPCG_02280"
     CDS             28284..28889
                     /locus_tag="ACZPCG_02280"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003497982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR1283933.1"
                     /translation="MNIMILSDQESKLLYEYYDPERIKDIDLLISCGDLPPEYLTFFA
                     SVCHAPLVYVRGNHDNKYAYKPPEGCICVEDDIYVYKGVRILGLGGSMEYIPGSANQY
                     TERQMRARVRKLRWKLLWHRGFDILVTHAPAFGLNDMEDLPHRGFKAFLTLMDKYHPK
                     FFFHGHVHANYGGKFKRKDTYGSTTVVNGYEFYVVEYPEGE"
BASE COUNT         7857 a         6141 c         7735 g         7324 t
ORIGIN      
        1 aatgggaatg acaacaacat ctgtatatat tgtattaagt gttctggttg ctcctgctct
       61 tgtagatttt ggagttgagc cgattgcagc gcatatgttt gtattttatt ttggaatttt
      121 gtcctgtatt actcctccgg tcgctactgc tgtgtttgct tcagccagcc aactgaatgt
      181 aagtccgatc aaattgggac tgtataccag tcggattgcg attcctattt atattattcc
      241 atttttattt gttctcaatc ctgcactttt gatgttggaa agcgctggaa tctggattat
      301 agcagagttt attttgtcag ctcttactat tattgctctg tgtagcagta tagagggatg
      361 ctttatgaca gaattaaaaa aactggaacg catactttta tttgtaggtg ctgtatgtga
      421 gttacataca tctgtaatgg ttaagctgtt ttcaaattta ttgatcgtta ttatactgct
      481 gagaaatata cagaagaaaa agcaaagaca gtaaaattcc acagataatg aaaattgaag
      541 ttgctggtat ccttgtattc tgcgtgctca ggtattaaga taaaagaaag cgaaggccgc
      601 tgcgaggctg aaaggaggag gtacttatgc ggaaacgact tgtagtattg ggggcttctg
      661 ttctgatggc atttgccatg gcgggaatgg cctatggggc agaatggaag gaagacgtag
      721 ccggatggtg gtatcagaat gatgatggaa gctatctggt aaatgaatgg aagtgggttg
      781 acggaaatgg ggacggaacg gcagagtcat attgctttga tgaacagagg tatctctatg
      841 tcaacacagt tacgccggac ggctatcagg taaatgagtc gggcgcctgg gtacaggatg
      901 gggcggcaca ggcaaaaacg gtatccatga tgcagtctgt tccgtcaaca gcggaaaatt
      961 atagtaacta tatggctttc agccactttc acagcacggt cgtgaataat ttaggctaat
     1021 ttacagattt attttatggc attggcattt ccagcgcatt ccccgtagta gggagcagga
     1081 agaacaatca gagaacattg acaggttcat cagcaaggta cggaaatatc ttgacttgga
     1141 tgaattgata tcccctctcc tgaatgacat ggtaaaggcg gtgtacattc acgcgtcgga
     1201 taaatcaaag gggaataggg aacagcagat tgatatttcc tatgaccttg tgggaatact
     1261 cgagaccgtc tttactgaat aacctataaa acggagaaac ggcatagccc gtaagctatg
     1321 ccgtttctcg aaaacatttc taatactgtt ttatgagtgc cgtcccaaag gcgcccgatc
     1381 ctgtatcaac cggtcaatga tcggattaag cgtttaatac gctctttaag tactctgcga
     1441 tctcaggtac ctggcagttt gcaaagaaat actcctggaa tttctctcca ctgatggcgg
     1501 tctttaagaa atcctgatcg atattcttta agatggtcat catatcggta tgggtgatct
     1561 tcttcacctc atccaggatc ttcttgttgc gctgctcagg aattacgcgc tcctttggat
     1621 agccgccgcc gctttcgcag ccgaaaagct tctcaaatac atactgtaag tgcagctcgg
     1681 caccccagcc aaagcccttg gcaaatggca ttgcgatggc gtttccgtca ttgatctgag
     1741 caaacatata agcatcctct acatctacga tatgaccgca gagtacgccg gggaaagagt
     1801 tgcaggctaa catagcgcct tctcctgtgc cgcagccggt aacgacataa tctgcagcct
     1861 tagagtttaa caggatagca gacagcagtc cgcacataac gtaggtcagc tgtgtttcat
     1921 cctcagcggt atacataccg tagttgaaaa cttcatggcc cataggctct acaatcttct
     1981 ttaagttctc gcagatcatg gaattctttg cagcctggct gttctcgttg atcagtgcaa
     2041 ttttcatagt gctttctcct ttatacatat agtgataagc cggaaggagc aaaatcgtcc
     2101 cccgcggcat atacattatc atactacaga ccggaaacac aggcaagttt tattcttcga
     2161 tcacctgtat ctccagatag tcaaaatcta ccgattcaat aaaatgatcc cggccaaagc
     2221 gggtttttgc atgtagctta aaatctttta tattggaatc cagccagatc ggcttttcca
     2281 ggtcaaattc atagcagatc tcatccagac agtcaaatac catctgggtc cgtgactggg
     2341 agtagtcgct ccggcaggca gttatatcct ttaacagatg gttgtccttt atgagcttgc
     2401 cccacattct gaacataaaa atcccccttt ctcatacatg gtatgcttca gtataacacg
     2461 gcaaaatgca gaacctcaag acttatattt ctgaaaaatc ctgctgtgcc tccgtcagga
     2521 tatctttgga atatccgtct tttgtatatg tatcaaaatc acaggctgtc cgtctggtaa
     2581 tgagagacgc cgtctcttcc cccagaatat cagagataca ggtatggaag ctgtttacca
     2641 ggtggccgca gtgatacaga aaatccttcg tatattgtcc ggccagttct tttttcacag
     2701 ctgcgatcct ctgacggctt ttgggagtct gacagtagcc tacataggaa aaagtgcagc
     2761 agctgtcgcc aagtcccagg gccgaccggg tgatcattgc cagatcggga tgaaagccct
     2821 ggatcagatg gagatctaca tatctgcagt acagtgcgcc gtagcgttcc agaccatatc
     2881 tggcccagct ttcctgccac atgcagcagt ggcagcagag cgtataaaca ggggactcgc
     2941 tgccaatact gtagtcctca ggacttgagg ggatttggag ttcgccatag gataaaaatg
     3001 tctcatgatc aagcctgcgt ccgtcccgga cggcccgttt tcgcatccgc tgtcccctct
     3061 catgggcata gcgcatgatc gcatttgcaa aaatatccag tccgtattct cccaaatgtt
     3121 cctccagtgt tttgccatat aatccgatca gaagtccgtg atgagttacg gtatatgtac
     3181 tgcgctccat agaatttagc agctcctttc tgtgacagtt atttttgtaa ggctattata
     3241 ccacaaaaca gatatggaaa agcagaccat ctcttgccca gcggcctgtt ttgtagtaag
     3301 ctatggatat acaaaagacg cgcggttttg gtatgatttc aggatgcgcg ggactcataa
     3361 ggaggacaaa attgtggcac aggccattac agattatgta aaattcctgg cagatgccag
     3421 agatgcggta taccgcgtga actgcgacag ccgtacagcc agacagctgg aagcagagga
     3481 gagccagaag gaaaaggagc tggcttcttt gaaaaaagag gtggcggata ccatttcccg
     3541 caccgtaaag gcacgcagag atgagattga atccggttat gacagagaaa ttgcaaaggg
     3601 gcaggacagg ctgaaaaaag ccagagctaa acgggaaaag gcaaaagata aaggcgtgaa
     3661 agaacggatt gccgaggaaa ccagccagtt aagagaccac aacagagatt tgaagataca
     3721 gttaaagacc atgtttaaaa aggatcgtgt tccggccttt gtaaatacga ccttctttta
     3781 tgccctttat tatcccaggg gaatgaagga atttggaata ggttttttaa ccttccttat
     3841 ctgctttctg gcattcccct gcggcatcta ttttttgatt cctgaacgaa aatggtggta
     3901 tctggctgca gtttatgtag cagatgtatt tttatttgga gggctttatc tgatggtggg
     3961 aaaccgtatt cggactcatt atcaggatgt tttaaagaag gggagggaga tccgcaatct
     4021 tctcagggcc aatcataaaa agatccatgt gattacccgt accattcaga aggacggaaa
     4081 tgaagctctc tatgacctgg aaaaatatga tgacgagatt gcctgcgcca gccaggagct
     4141 ttctgaaatt gcagagcgga aaaaggatgc tgtgagcaat tttgaaaatg tgacaagaac
     4201 cattatttca gatgagatcg agggaagccg cagagaagaa atgctggctc tggaagagga
     4261 gattaaggag ctttccgccc agcttcacag cctggaggac agtatccggg atcagaacat
     4321 ccatatcaca gatacatttg gcccttatct tggaaatgaa tttttagaac ccgaccgctt
     4381 aagcgagctt tcaaagctga tccagtcagg ctccgcggcc aatattacag aggctattac
     4441 cctgtataag gctggaagcc agaaaaaggc ggatggacag tagaagaacg atagatattt
     4501 ttaaacggaa aaggccccat accggtgcat cctttctggt gtatttcaga gaagagatgc
     4561 aggtatgggg ctttggattt gtttacaaag tcagatagtg aaaatccacc tgattcgctg
     4621 tgagcagctg ggcctctctc tggtggcagg taaagagaat gatctgtccc ttaaaggtct
     4681 ttgagagcca ttttaaggca gttttcagcc gttcatcgtc atatagagca aagctgtcgt
     4741 caaagattaa gggcatctgt tctccttccg tctgaaccag ctttgcggcg gcaaggcgga
     4801 ctgccagata aatctgatcc atggttccgc tgctgacctg ctccacagga acaagatgag
     4861 agggggtgtt cataaagata tttaagtcct gatcgatact catactggtg tagataccgc
     4921 ctgtgatacc cgctataagc tcagaagcag ttttatttaa gtagagaccg aaggaatccc
     4981 ggatggtaga ggaaagccgg tttaaagtat cctgggccag atccagggca tccagctcct
     5041 cctgaatcct ctcattttcc gtgagagcgt gcttcaggcc ttccgcttca ctgcggcagg
     5101 tactgagccg ttccagtttt tgttctaatt cccactgaag gcgctgaccg gtttcgattt
     5161 cctttgaaag ctctgcttca cgggactgga gctgttcggc ttcatctgtg agccgcttca
     5221 gggcgttctc cgactgatat acagcccgct ccaggcgaag gagctccgat atgcgctgtt
     5281 ctaaccggcg gatggattca tcggagatag aggaatcgcc cagatgatcc ctgagaatcc
     5341 cggataaaaa atccgttaca gaggacagct ctcgttttag gcgccgtctg ctccatagct
     5401 gcatcagggc agctgccagg aagacagcgc ctccaaggcc gcagagaccg gtgagaaatg
     5461 taaaaaaggc aggagaggtt ccagcgtgtt caggcccgaa ccgggtaaga agcccccgga
     5521 tcatatctgg ttttttgatg caggatccca ttcccagagc caggatgcct gccgccagaa
     5581 aacagagggc tgtggctgcc gtctgaacgc gtccgccgga tgccgccgtt ttttctctgt
     5641 attccttata ggcttcctgt gtctggcgaa gctggttttg aatggaggcc tcatctgtaa
     5701 aatggcttcc aataaggatc tgccgtcctc tggcagtttt ttcaagaaga gactcttttt
     5761 ctgtccggtg ggtttcaagc tcttccttta tctgcgccag atcagcctga caggatgcga
     5821 gacggttttg gtagcgggga tcggaaattt cgtgttcaat ggtgcgtact tcgctcagaa
     5881 gcgctgtgta tctctttgcg gcctcaggta ccagctttgc agccatttcc cgcctctggg
     5941 attttagaag agcgctggct ttggtgatat ttaaagcaat actgccggag gtattgagat
     6001 tggcaatgta atttcgcagc tccgatacca tccccccgtc tgtggcgctt tttaactgtc
     6061 cgatgctgat ggtgttgctg taggcggttt ctgaaaggcc gcaccgcagg cgttcccaca
     6121 gagccgctgt gggcgtttcc tcacagccct ttgtttcatt gatgatcgta aggcttttat
     6181 tccctttttg aaatctgcgt tcaatccgga agatttctcc ctggctttcc agacggagcc
     6241 atccctgata agcccctgta ccggaccatg gttcaaagcg ggaataggta tcatttttgg
     6301 aagcacgtcc ccgctggcgt tcaattccaa agagcattcc gcggataaag gtatgaaggg
     6361 tggattttcc ggcttcattt tttccataga gcacatttaa gccgtcttca aaggaaagat
     6421 ccaggtcatt cagttttcca aagccgtcaa tatgaagact gagtattttc atgggtctag
     6481 ctcctttcgt ccgtggtgtg aagcagggca tttacgccat aataaagggc ctttttttct
     6541 acaggtgaca tatcctcttt ttggagtgcc tggataaaaa agccgatcat atcgctggaa
     6601 tgttctgcaa aaagtgctgg aaagtcatag agcggttcgg attcgtcgat gatttcaccg
     6661 attcggaatc gtgcagagag ggaggataga tcaaaggtaa tatccggatc cctcattcct
     6721 tttaaacgga agcggtaaat atgctcccgg ccccgtttgt cgatttcatt gatgatccgg
     6781 tcggtcagac ctccgtttgt cgtttccgtc gtgacggtca cagccagagg gatgcagcgc
     6841 aggctggcgg cagggataaa gtccagacgg gtcagccgcc gggtaacggg gtggatttct
     6901 ccttcaaaat atccgtgggt tcctgtctct gtcagatcca ggggttcagg ggaaccgcag
     6961 taggccattt tatcctcggc aatgacctgc gctttgtgga tatgtcccag tgcaatgtaa
     7021 gaaaatgggg ataagctcag ggtgttgaaa ttcataggaa catgggccgg gtctccgccg
     7081 tgggctaaaa ggatctggat ccggttgttt aagggaacct ggatatcctc taagagaggc
     7141 tcgcgtattt cagtggtatg gtagctgaag ccatagacct ctgtgttaat gtcatcaaag
     7201 taaacagaac ttaaagcggg atcagcaaga aaggttacat tagggctcca ggaaaaactc
     7261 aggatactgg aattttcccg gatcttgtcg tggtttcctg caatcatcac gaccctgaca
     7321 tgggggattg tggaaaagag ataattgact tctttgatat cccgcatcag aggctggcgg
     7381 tgaaaaagat cgccgctgat aaacaggcag tcggctttca ctgcgcctgc atgatcgata
     7441 atggctttaa acgtatcccg gatggcttgt gcccgttctt ttccccaggg tttatctgca
     7501 tccggggtca tgccccaatg tacatcgcct gtatggatga atttcataat acctccccct
     7561 cgtccggaag cagaggatgc ttccaacgct tccataaaca tcagggcctg ttatcgcaac
     7621 aggccctggt ttgatataga tgctgcctgc agccgaaacg cagtccggac tgcagatcat
     7681 ggataaaatt acagctcgca gttatttaca gttcttggga acggaatgac gtcgcggatg
     7741 ttggacatac cggtcaagta catcacgcag cgctcaaagc ctagaccaaa accggcatga
     7801 cgggcagaac cgtatttgcg cagatccaga tagaagccat aatcctcttt cttaaggcca
     7861 agctcctcca ttctggcaac cagtttatcg tagtcgtcct ctctctggct gccgccgatg
     7921 atctcgccga taccgggaac cagacagtct acagccgcta cagttttgtt gtcgggattc
     7981 atcttcatat agaaggcttt gatttccttt ggatagtctg taacaaatac aggcttcttg
     8041 aaaatctgct ctgtcaggaa gcgctcatgc tcggtctgaa gatcgcagcc ccaggatacc
     8101 ttgtaatcga actggtcatt atgctcctct aacagtttga ctgcctctgt ataagtgatg
     8161 cggccaaact cggagttcag tacattgttt aagcggtcta acaggccctt gtccacgaac
     8221 tggttgaaga agttcatctc ctccggtgcg tgctccatta caaagcggat aatgtatttt
     8281 aacataccct cagccagatt catattgtct tccagatcag cgaaagcaat ctcaggctca
     8341 accatccaga actcagccgc atggcgggta gtgttggaat tttccgcacg gaaagtaggt
     8401 ccgaaggtat agatattgcg gaaggccatg gcgtaggttt cgccgtttaa ctgtccgctt
     8461 acggtcaggt tggttggctt tccgaagaaa tcctgcttga agtctacctt gccgtcttct
     8521 gtctttggaa tattgtttaa gtccatggta gttacctgga acatctctcc tgcgccctcg
     8581 cagtcgcttc ctgtgatcag aggggtgtgt acatagacaa aatcacgctc ctgaaaatac
     8641 tgatggatgg cataggcaat cagggaacgt acgcggaata cagcctggaa ggtgtttgtt
     8701 ctgggacgca gatgggaaat ggtgcgcagg tactcaaagg agtggcgttt cttctgcagc
     8761 gggtaatccg gtgcggaggc tccctctact gtaacttctg tggcctggat ctcaaagggc
     8821 tgctttgcct gaggcgtagc caccagagta ccctttacaa tgacagctgt tcctacaccc
     8881 agcctggata cctgctgaaa gttctccagc gtgtcgtgat aaacggcctg cagaggctca
     8941 aaataggttc catcgctgat gacgatgaag ccgaaggact tggaatctct caggctgcga
     9001 acccagccgc cgatggttat ttcctgatcc atatactgat ccggatgctt gtataattct
     9061 cttactgtaa tcagatccat aataatggta acttcctttc tttttccatt gatttcgata
     9121 aaaaaatatg tcttaaaaac tgcatatcgt gattatatga tatttctctc aatgtttcaa
     9181 ggccttccca gccggagttt ccgcattttt ctgcaagttt aaggcgctga aggaagagac
     9241 tgccctggtt tgcatgtcag atccggaagg ccgtttagat ccggataaag gatatatagt
     9301 atttaaaact gtaccataat acaagatatg gataaattag aagaactatg gaatttttaa
     9361 acgaaaaatg tcggaaaact gcgcaaaaaa cacaaaataa aattaaatat ttatatacgg
     9421 aaactaaaat ttgtgttata attatactta caaaaattat aacagcaaga ggtgataacg
     9481 tgattaagaa agaaatgatt gccatgcttt tagctggcgg acagggaagc cgccttggcg
     9541 ttctgacaaa gaaagtagcc aagccggcgg tatcttttgg ggggaagtac cgtatcattg
     9601 attttccgct gagcaactgt atcaactccg gtattgacac agtgggagtt ctgacacagt
     9661 atcagccatt acgtttaaat acccatatcg gaatcggtat tccgtgggat ctggacagga
     9721 acgtaggcgg cgtgacgatc ttacctccat atgaaaggag caaaggcagc gactggtata
     9781 ccggtacagc caatgcgatt taccagaatc tggaatatat ggaagcgtat aatcctgagt
     9841 acgtgctcat cctttccggt gatcacattt ataagatgga ttatgaggtt atgctggatt
     9901 atcacaaagc gaataatgcg gatgtcacca ttgctgccat gcctgtgcct attgaggagg
     9961 ccagccgatt tggtattctg attacggacg aggccaaccg gatcacagag tttgaggaaa
    10021 agcccgctgt tcccagaagc aaccttgctt ctatgggtat ttatattttc agctggcccg
    10081 tattaaggga cgctttaatc cacttaaagg acgaacccgg atgtgacttc ggaaagcaca
    10141 ttattccgta ctgtcacgga aagggagacc gcatttttgc atatgaatac aacggctact
    10201 ggaaggacgt aggtactctg ggatcttact gggaggccaa tatggagctg atcgacatta
    10261 ttcctgagtt taatctttat gaggaatact ggaagatcta taccaagagc gatgtaatcc
    10321 ctcctcagta catagcagat gatgcagaaa tcgaacgcag tatcataggc gagggaacag
    10381 aggtttacgg gaaggtgatc aattctgtta taggttcggg agttaccatt gagaaaggag
    10441 ccgtagttcg agattccatc gtgatgcagg gcactcatat cggagcctgc acagtggtgg
    10501 acaaggccat tatcgctgaa aatgtaacga tcggggcaaa tgccgaggta ggctttggag
    10561 attatgcacc cagcacctat gatcccaagg tatatcagtt tgaactggct accatcggag
    10621 agaattcagt gattcctgac ggcgtgaaga ttggaagaaa tacagccatt gcgggcgaga
    10681 cggcaatcga cgactatcct gacggtatac tggcaggcgg aaattacatt ataaaggcag
    10741 gtggcgtgaa atgagagcaa tcggtattgt tttagcaggt ggaaacagca agagaatgcg
    10801 ggaattgtcc aacaagagag ccgtagccgc tatgccggtt gcaggaagct accgcagcat
    10861 tgactttgca ttgagcaata tgaccaattc ccatatccag aatgtggctg tatttacaca
    10921 gtacaattcc agatccttaa atcttcattt aagctcgtcc aagtggtggg atttcggacg
    10981 taagcagggc ggactgtttc tgtttactcc atccattact ccggagaacg gcgactggta
    11041 ccgcggaacc gcagacgccc tgtatcagaa ccttacattc ttaaagaaca gccatgagcc
    11101 ctatgtagtg atcgcctccg gcgacggcgt gtacaagctg gactataaca aggttctgga
    11161 ataccatatc gagaaaaagg cagatatcac cgttgtatgc aaggatatgg aagaaggtat
    11221 ggacgtcacc cgtttcggct gcgtgaagct ggctgcagac ggacggatcg tggactttga
    11281 ggaaaagccc atggcttcag acgccactac gatttcctgc ggtatctatg taatccgcag
    11341 aagacagctg atcgagcttc tggagcgctg tgcagcagag gaccgctatg atctggtaaa
    11401 tgatattctg gtgcgctata agaacttaaa gcgcatctat gcatacaaga tgcacagcta
    11461 ttggagcaac attgctaccg tagattccta ctataagacc aacatggatt tcctgaagcc
    11521 ggaagtgaga aattacttct tcagagagta tccggatgtc tataccaaga tcgacgatct
    11581 tcctccggca aagtacaacc cgggcgctgt ggtgagaaac agtcttgttt ccagcggctg
    11641 tatcttaaac ggcacggtag aaaattcgat cctgtttaag aaggcttaca ttggaaacaa
    11701 ctgtgtgatc aaaaactcca tcattttaaa tgatgtatat atcggagaca atacggtgat
    11761 tgaaaactgt atcgttgaaa gccgtgatac catccgggcc aacaccacac atatcggaac
    11821 gccggataat atcaagatcg tggtagagaa gaacgagaga tatacattat aatttacgcg
    11881 cagtcatctt cctgctgtgc gtggaagatg cagcaagggg gctgacggag aggacggaag
    11941 gggattagca tgcaggttac ggatgtaaga gtcagacggg tagaaaaaga aggtaaaatg
    12001 aaagcgatcg tatctattac catggataat gaatttgtga tccatgatat taaggtgatc
    12061 gagggagaaa aagggctgtt tatcgcgatg cccagccgcc gttctgcaga cggcgagtac
    12121 cgcgatatcg ctcatcccat caattccggc accagagaaa tgattcagaa tactattctg
    12181 gagaaatatg agatgacgct gctggagaat gagggagcag aggctgaata accggataat
    12241 attaagaacc ggacagaagg attgaattct ttctgcccgg tttttttgtg tggtgcgcct
    12301 ggcggcgcac gtcgccagcc atgattaaaa gagggatttt ggtgtacttt ttgccgagta
    12361 ctgctggaag aaagccggat ggaactggaa atgattttgg ggtatactac gcataacaaa
    12421 cagacagtga gtataaaaat caggaatgta cggaggcaga agggaggaat accggatgca
    12481 gatggatgga cgcctgaacc ggcttttgtc agtgctttca gaagatttat atataaatat
    12541 ggaaaccgtg gccgaccagc tggaagtcag cgagagaacg gcccggacgc tggtcagtca
    12601 gctggatgaa cttctttgcc ggaacggagc tgccatagag cgccgcagag gtcttggact
    12661 caggctttgc gtaaaggaac cggagcgcta tcaggagttt ataaagggaa gaacctcagc
    12721 cctgcttccg gagaccggaa aagagaggat agaatttatt ctggcccgat tatttgccga
    12781 tgcttccgga gtgaaggcgg aggagctctg cgaacggctc tatatatcca gaaaaacgtt
    12841 gtcccttgat ctaaaaggag tcgagcagta tttaaataag catcatctgg ctcttgaaag
    12901 aaaaccctat tacggtctta agatccaggg ggatgaattc tccatacggc tttgtatgag
    12961 cgcggcattt tatgagttca gcgatcagtg gtttaaggaa atttatcata cattttggga
    13021 tggggaacag atccgcaatg agattctaag cagtgtaaaa caatgtgggg aaaccatcta
    13081 tgaaatggat atccctaata ttgtcctgca ggtgcagatc gctctgtacc gctggggacg
    13141 gggctgtgtg attccgttgg aggagatgaa gcacagcgac ctgcttcatg aaggcgatat
    13201 ccgtgccgca aggctttgtg cggaaggact gaaagaggct tttggaatct gtcttccggt
    13261 tcctgagatt aaatacatgg cgattcagct tctgggaaag aaaaagattt tggccggaga
    13321 cagaagcagc gtttttattg atatggagat caatcagctg gtgaaccgga tgcttgaaag
    13381 cgtcaagcag acatataatc tggatctggc atcggatttt gatttaaata catccctgag
    13441 acttcacatg gtatcgctgc ggatccgcct gcagtaccgg ctgaaaatgg acaaccccat
    13501 tttaaaggag attaaagagg tatacagctt tccctatgca gtggcggccc atgcgtctac
    13561 ggttttgtcg gagtatttcc acaccatagt ccctgaggca gagattggat acctggccct
    13621 gtgttttgcc ctttcgttaa aacgacagga cagacagagg tacaagagga atatccttct
    13681 tgtgtgcgcc tccggttccg gtagtgctaa gctttttgaa tatcggtttc gggaaatgtt
    13741 cggagattat ctggaccggg tagaagcctg tgatatcgga tctctggcat cctgggattt
    13801 ttctaatata gattatgtat tttctacggt tcccgtaaaa acagccattc cggttccagt
    13861 gtgtcaggta cagtattttt ttgacaggca caatgcggga gaggtggaac ggctgctgaa
    13921 aagcagcagg aaaggcggaa tccgatcgta ttttgaccgg tcgctttttt ttaccaatat
    13981 aaagggaaac agcagagagg agatcctcca tgagctgtgc gggcgcatcc gcagagtgcg
    14041 ggaacttccg ccggattttg aggattccgt acgaaagaga gaaaatctga tgcagacaga
    14101 cttctgtcct cagattgcaa ttcctcatcc ctatcagcca gtgacgaagg atacttttgt
    14161 ctgcgtggcg cttttggaaa agccggtgct ctggcatatt tatgaggtac aggtggtatt
    14221 tcttttatcc gtttcggtta ataaggaaaa cctggaggaa ttttaccgga ttgcccccag
    14281 ttttatgatg gacgagggct gtatgaacca tcttctggaa acccggagtt atgataccct
    14341 gatggatatt attgactggg cggagagaga tgagcttcag aaccggtagt atataaaaca
    14401 cattaaccaa actaaggagg taacaattat gggtacgatc agagtgttat tatgctgcgg
    14461 agctggattc tccagcggat tcctggcaca gaaggcaaga caggcagcaa agaagaaaaa
    14521 gctggacatg actgtggaag ccagaagtga gagtgtggta agcgagtaca tggacaagat
    14581 ggatgtactg ttagttggtc cccactatgc aagcgctgtt ccgaagctga cagaggagtg
    14641 cgcaccccat ggaatccagg tagagctgat cccggcagag atttattctt ctctggacgg
    14701 agccgcactg gttgagctgg cagagaaggt agctgcaaga aaggatggat aagtacagat
    14761 gaatggttta atgacatggc tttcggacgt atttgctcct aaggcccggc aggtttttgc
    14821 aaatccatgg atcgataccg tggcaagtac catgaagaag gttcttccga tcattttgac
    14881 cggttccctg atcttcttct acaacgtatt ccgttcctat atcccggcgc ttcctgattt
    14941 aagtcccatt gcgaatttct cctttggact gctggcaatc ttcgtagctt tctttgttac
    15001 ccatgaggcc atgatcaagc tgggacatgg acagtatcag atcaatgcat cgctgatctc
    15061 cattgcagcg tacctgctgc tttgccgtcc tcaggtagta gacggaatct tccagattga
    15121 taacggccgt atcggcgctg ccggtatcat gatgggtatc gtagtaggtc ttttcgttgc
    15181 cattgtattc cacatctatg caaaccttca tgtactggaa aacaatgata cgcttccgga
    15241 ttttgtaagc gactggatca acaatatcat tcccattacc attactctgg gactggcgat
    15301 gatctttgca aacgtattaa agctggatct ttacaatatt ctggtaagct tctttatgcc
    15361 tcttcagaag ggcgctcaga cacttcccgg ctttatcctg atctgctttg taccggttat
    15421 tttctactca atgggtattt ccagctgggt attcaatgca gttaccactc ctatctttat
    15481 ggtggcgatc gcagagaata tcggggctgt ggcaaacggc ggcgttccta tgaatatcgc
    15541 taccagcgag gccgtattta cagctgcttt gatcaccatg ggcggccgcg gcggcacact
    15601 tcctttaaac gtgctgatgc tgggttccaa gagcaagaag cttaaaacca tgggtaagat
    15661 ctgtatcgga ccttctttgt tcaatatcaa cgaaccgtta atgttcggcg caccggttgt
    15721 atttaaccct ctgttaatgc ttccgatcct gatcaatagt atcgttggtc ctacagttgt
    15781 atggctgacc atgcactatg gcctgttaaa tattccttcc aagatggttc aggtaggaca
    15841 gatcccggct ccgttttcca gtgtgatgat tacgggagac tggagagccg ttccggtgta
    15901 tatcgtactg tttatcctgt atctgctgac ctggtatcca ttctacaaag tatatgagag
    15961 acagtgcgta gaggaagaaa cacaggctgc atgctgatgc agagcactta aaacatatgg
    16021 cggcgttccc tggttttggg ggcgctgctt ttcagaaagg agagaaataa aagtgggaga
    16081 aatgatgttt aaggatgatg ataaggtcgt tcaggttgcc atgcagatcg taatttctgc
    16141 aggtgatgcc agaaatgcag ccggaaaggc attagattgt gtagcagcat ttgattttgc
    16201 aggagcaaag gagcatatgg aggaggcaaa taaccatatt tgtgcagctc acaatgctca
    16261 gacagagatg atccagtctg agatcgcggg aacagaaacc attcagccaa gcctgctgtt
    16321 taaccatgca caggatacgc tgatgaccgt tatgtcagag atccacctgt cagagaaaat
    16381 gatccaggta tttgaaagtt tctacaataa aatgaatgca taaaggagga actatgagcg
    16441 cagatatcag aaagacgatg ccggaaggtt ttttatgggg agcttctaca agtgcttatc
    16501 aggtagaggg agcagcagaa gaggacggaa agaagctgtc ccagatggac attttaaacc
    16561 gcggaaccgg ctttgcagat gcaagcgtag ccagtgatca ctatcaccgc tataaagaag
    16621 acatcgcgct gatgaaggaa tgcggcttta ctgcataccg tttttccttt tcctggtcaa
    16681 gaattttccc ggacggcgtt ggagaagtaa acccaaaggg cctggagttt tatgacaacc
    16741 tggtaaatga gctggtagca aatggaatta agccgatccc taccatttac cactacgata
    16801 tgccaatggc tcttgtagac aaatatgacg gctggataag ccgccagagc gtggatgatt
    16861 ttgtggctta cggagagttc ctgatccgca gatacggcga caggatcaaa gactggctga
    16921 ttatcaatga gcagagtatt attgtggaat tctggaaaaa gaagaattat gtgcctgaga
    16981 aataccatgg ggatcctcag gtgaaattcc agatcaacca ccatatgaat ctgggacagg
    17041 caaagatcag caagctgatc catgagctgg tagaaggcgg acgttccggt gcagccatcg
    17101 gatattctcc ggtatacgga ctggattcca ctccgaaaaa tatgctggct atgttaaatg
    17161 cagaggattt aaagaaccac ttcttcctgg atatttattt taagggcaaa tatcccgtag
    17221 gtgcattccg ctatctggaa gagcatggaa tggctcctgt gatcgaagag ggagatatgg
    17281 agattatccg tgagggaatg ctggacttcc tgggcgtaaa ctactatgca agcaagtgcg
    17341 tgcgcttccc ggatgctgat aagaatgagc tgacagaggc taagagcaat ttaagcggca
    17401 agaagggcgc tatgggagct tatgaggtaa tgcctgattt ctatcagtac atgaagaatc
    17461 cgaatgcgga tacaaatgac tgggactgga ctattgatcc aatgggtatg cagtaccttc
    17521 tgcgtgatat ttatcagcgc tatgagattc caatgatcat cacagagaat ggtctgggag
    17581 caagagacgt actggaggac ggaaaggtac atgatgatta ccgtatcgac tactggacga
    17641 agcatcttca ggcaattcac agagctattg agctgggagt ggatgtaaga ggcttcctgc
    17701 catggtcctt tgtggatgta ctcagcacca gtaatggtta ccagaagcgc tatggtctgg
    17761 tgtatgtaaa ccgtgatgat gaggacatca aggatctggc aagaattcca aaggatagct
    17821 attactggta tcagaaggtg atcgctacca atggggcaca ttgtctggaa gaagaataaa
    17881 gatataaaaa ggccgtccag ccggaatcgg aaaagtcgat tccggctggg cggtttttac
    17941 atcctcttca aaatacttct cgccacgctg ataccgtttg cagaagcctg ctgcagtcct
    18001 ctggtaacag aagcgccatc gccgatggct ctcagtccat gtacgctggt ttcaaagtct
    18061 ttatcaacca ctaccttgtt ggagtagaat ttaacttcta cgccatagag cagggtttca
    18121 tcgctggcaa taccgggagt gaccttgtcc agggccagga tcatttcctt gatatccgtc
    18181 ataatgcggt aggggaatac aagggacaga tcgccgggaa ccgcatcctt taaggttggg
    18241 atcaggttgt tgcggcagag gcgctcctct gtggtgcggc ggcctctctg gaagtcgccg
    18301 aaggtctgga ccatgatcct gcctccgcag agcatattgg agagctgggc aatgtgttta
    18361 ccgtactcga tgggctcgtt aaaggggcgg gtgaagtttt tgcttaccag aagggcaaaa
    18421 ttggtattgt ttgtcttata ttcctgtgat ttatatgcat ggccgtttac tactgcaagg
    18481 ccgttttcat agtattcggt tgctacttca ccggaagggt tgctgcagaa ggtgcgtacc
    18541 ttatcgtcaa aggtaggggt gtagtagacc agcttggcct cataaaggtt ttcgttgagg
    18601 aatttcataa cctcgtcgcg gacttcaacg cggacgccga tatccacggt tccaaccttt
    18661 gtctcaatgt ggtggttatc gcagacatgg gagaaccagt cagaaccctc gcggccaatg
    18721 gcagctacga tctccggtgc gcagaaggtt tcatctttgt ccgttacaat accctttacg
    18781 gtatcgccct cgatcaggat gtcctttacc atggtgttaa atttcatctc aatgccctga
    18841 gccagcaggt gctcctgaag gcgggtatag attttgtagc cttcctctgt acccaggtga
    18901 cggatggggc attcgatgag ctttaagttg gcgttgatgg ccttgcggcg gatttcttcg
    18961 atttccttct gcttgtcaat gccgtataca tttttgtctg ccccgaattt cagataaatg
    19021 tcatcagatt cctttaaaag cgcagccgtc tcgtcatacc ccaggatttc gggcaggttt
    19081 ccgcctacat ctggagacag tgaaagcttt ccgtcggaaa atgctccggc gcctgcaaat
    19141 ccagttgtga tggaacaggg cttgcagcct acacagacct tggtagtgcg cttggggcag
    19201 acacgttttt caatgggacg gcccttttcg accataagga tcttaagttc cggtttctga
    19261 cggatcagct cataggcgca gaagatgccg gaagggccgg cgccgatgat gattacatca
    19321 tacagattat ttttgctcat aagaaattct cctttctttg agattcaaag cctgatggta
    19381 aataaggccc agaacgaata taactatagt ctatcatagt tatgaaaagg ggtctaccgt
    19441 tcttttttca ttacccgtgt taccataaaa atacagtaaa acatggctaa ggcccatgca
    19501 atgggattgg ccagacagat gccaagatat cccagctttg ttactgccag aaggcctcca
    19561 aggcttcgtc cgaccagctc gctgatgccg gatataacag cctgcatact gtatccaagg
    19621 ccctgaaggg tatttctgaa gatcattaag aggccgtgga agcagaacag aaggctcata
    19681 acgcttaagt attccagcgc tccggcagtg actgccgggt ctgcgtctcc cagcaccagc
    19741 ccgacggcaa aatctttgca caggaaaatg gcggtccagg cggcagcgca gtagcacagc
    19801 tggattttgc agccgtcctt tataccctgg cggatgcggt cggtgcgccc tgcaccgtgg
    19861 ttctggccgg cataggttgc catagccata cccacgcttt ccatgggaag ggtgaacatc
    19921 tggcggattt tttctcccgc ggtctgtgcg gctacggcag tacttcccaa gaggttgatg
    19981 gcgtcctgca taataacggc tccaatggcg gaaacggagt attcaaagcc catgggaagg
    20041 ccgatgtagg ccagcttttt gcagtggtgg gaggaaaggc gcagcttatt tttagtaaag
    20101 ctcaggccgt gtccttttgc agttttggag aagaaccacc aggtacagag caggccgctg
    20161 agggcctggc tgattacagt agcgatggct gctccgtcta ctcccattcc gaaaggaacg
    20221 attaaaatcc agtcaagggc aatattcagg agactggagg ccaaaaggaa ataaaaggga
    20281 tgtctggaat ctcccatggc ccgcaggacg ctggaggagt agttgtacag aaccgtagca
    20341 ggaattccca ggaaaatgat gcggatataa agagaggcat catcaaagag ctcaggcggc
    20401 gtgtggatga gctttaggag cggaggggcc gtcagggtgg tgacaacagt aaagacagcg
    20461 ctgagcccaa tgcagagggc ggctccgttg atgaggaagc gctccagatt tttatgatcc
    20521 tttgctccaa aggattcggc tgccgggatg ccgaatccca cacaggctcc ctggataaag
    20581 ccaaggatca gaaagttcag ggagtaggtg gttcctacag ctcccagggc gtcggttccc
    20641 agacagcggc ctacaataat ggtatctgca aagctgtaga gctgctgaaa aagtgtaccc
    20701 agcaccagag gaagggcaaa acgaaggatc tgagccaggg ggcgtcccac ggtcagatcc
    20761 gttgtaccgg aacggttttt tttaactgtt tccatgataa aaattctcct tgtgtgtttt
    20821 acggctctat tttagcatgg attccagatg aatggggaat atctttagaa aatatttcag
    20881 aaagcgtcag atttatgtta gagttcttta aggctttctc aaggacacaa gggggagaat
    20941 gtgctagaat gatattataa gaaataaacg tacgaaagag aaagaaggtg gacagatgag
    21001 aaccggtaaa aactggaaca tattaaaaag gggtcttaag tggaccattg gcatcatggc
    21061 ggcagcggtt atttgtatct gcgtaaatgt aggcatgaag ggctatgata tgtacagaga
    21121 tgccatcagc cagatcagcc ttaaggacaa ggtagagcag atccgttcca aggaaaacta
    21181 tacggaattt gcagagcttc cggaaacgta tgtagatgcg gttctctctg tggaagacca
    21241 ccgcttttac ggtcatatcg gtattgaccc catcgccatt acccgtgcgg ttatcaatga
    21301 tataagggca ggcgcttttg tggaaggcgg aagcaccatt acccagcagt tggcaaagaa
    21361 cctgtatttt acacaggaaa agaagatgga gcgcaaggcg gcagaggtat ttatggcttt
    21421 tgctctggaa aaggaataca gtaaaaatga gattcttgag ctttatatga atactattta
    21481 ctttggaaac ggatactact gcattaagga tgccagtgag ggttactttg gaaaggctcc
    21541 tgaggatatg gacgattatg agagtactct gttagccggt gttcccaatg ctccttccca
    21601 atatgccccc acagtaaatc cggcactggc tgccaagcgc caggagcagg tgctggagcg
    21661 tctggtggag tgcggactct ttacagaaaa acgagccaga gaaactatgg cggagaccag
    21721 ggaatatcag gaaccggatg cggcgtaaag gctggatggt cggatctgcc gcactgcatt
    21781 tacaatccac ttataatttg atagaataaa gaaaagataa gcgttagaca ccccatgtgc
    21841 gggagtctag cgcttatggg cgtgtctgta cacggcggac atatggacag acactgcagc
    21901 accgcaggca tttttctgcg gaagaggata aattttgaga taaagaaaga ggaaaacaga
    21961 tatatgtatt tgatcgttgg tttgggaaat cctggaaggg agtatgataa gacaagacac
    22021 aatgcaggtt ttgataccct ggatgtgctg gcagatatgc tcaatacatc ggtggaggag
    22081 aagaagttta aggggcttta tggcagaggc ctcatcggtg gagaaaaggt aatcctttta
    22141 aagccccaga cctttatgaa tttaagcggt gaaagtgtca gggaggcggc ggatttctac
    22201 aaaatcgatc cggagcatat cattgtaata tatgacgata tcagtctgga gccgggacag
    22261 ctccggatcc gcagaaaggg cagcgccgga ggacataatg gaataaagaa catcattgcc
    22321 catctgggaa cccaggaatt tccccggatt aaggtaggcg taggggctaa gccgcccaga
    22381 atggatctgg cggactatgt gcttggccgt ttttctgctg aggacagaag ggtgatggaa
    22441 gaagccttta agacagcggc gaaagcagct caggtgatga tcacagacgg ggctgaggcg
    22501 gcgatgaatc agtttaatgg gcataaggag tgagtttccg cacattacag ctcaccccgc
    22561 agtctgatac ccagagctcc ggtttccgac cataagaaaa cctaggagac tgagattttt
    22621 ctaaggttta ctaatagtct ccaaagtcgc tctgggtatc agacagcagg tcacactgta
    22681 atgtacggaa acttcaatat tatcaaggaa aggcagaaag ggaaatcatg gaaaatccgt
    22741 tactggaatt acaggagtat gaaaatcttc tggaggcttt aaaaaaggca aaggggcctc
    22801 tgcaggcggc cgggacgctg gaatcccaga aggttcacct catgcatgag ctgggggagg
    22861 acgggggatt tccctggaag ctggttgtta cctatgatga gcagagggcc aaggaaatct
    22921 atgatgattt ccgcagcttt acccggcagg tctggcttta tccggccaag gatctgcttt
    22981 tttatgctgc ggatattcat ggaaatctga tgactcggca gaggatctct gtactgcgcc
    23041 atctgatgga ggatgagggc ggtgtagttg taacgacaat cgacggtctg atggaccatc
    23101 tcatgcctct ggcattttta aagcagcatg cagtgaccgt ggaatgcgga caggttattg
    23161 agctggatca gtggaaggag cggcttacag agctgggata tgagcgtaca ggccaggtag
    23221 acggtatggg ccagttttcc atccggggag gcatcataga tatttttccg ctcaccgagg
    23281 aagttccggt gcgcattgaa ctgtgggatg atgaggtgga ctccatccgt acctttgatc
    23341 cggaaagcca gaggtcggta gaacagttag accgggttgt aatctatcct gcatcggaaa
    23401 ctgtcttaac gaaaggacag ctggagaaag ggataaagcg tttggaggag gaaacggcct
    23461 cttatgtgaa ggctttggag ggacagaaac gccgtgagga agccatccgg attaagggaa
    23521 tcatacggga attgacagaa gggatccgtg agggctggaa gagaggcgga ctggatggct
    23581 atatccggta tttctgtcca gaaactgtat cattttccga ttattttcct caaggggact
    23641 gtgttgttta tttagacgag ccgggacggc tgaaggagcg gggagaaaca atcgagctgg
    23701 aatttcgtga aagcatggtt caccggttgg aaaagggata cctgctgccg ggacagacag
    23761 agcttctcta tcccgcagga caggttctcg gaaggcttca ggggccgtac agcattatgc
    23821 tcacagggct ggatcagaag ctgccgggaa tgaaggtgaa ccataagttc agctttgatg
    23881 tgagaaatgt aaattcttac cagaacagtt ttgaaatttt aattaaggat ctgacccgct
    23941 ggaaaaagga aggctaccgg gtggtgcttt tatctgcctc ccgtacaaga gccagccgtc
    24001 tggcagggga tttacgggaa tatgatctgc gggcctactg tccggacgga gagacggaag
    24061 gagaggaaag ccgcgcagaa cggaaggttc ttccgggaga gatcatggtt acctacggaa
    24121 atctccaccg gggctttgaa tatcctctgc ttaaatttgt tttcattacc gagggcgata
    24181 tgttcggggt ggaaaagaag aaaaagaaac ggaagaagca gagctatcag ggccgggcga
    24241 tccagagctt ttccgagctg tccgtgggag attatgtagt ccatgaggag cacggccttg
    24301 gtatctaccg gggaattgaa aaggtagagc gggacaaggt ggtaaaggat tatatcaaga
    24361 ttgaatacgg agatggaggc aatctgtatc tgcctgccac ccggctggag agtatccaga
    24421 aatatgcggg ctcagaggcg aaaaagccaa agctcaaccg tctgggaggc tctgaatgga
    24481 caaaaaccag ggcaaaggta aagggtgccg ttcaggaaat cgcaaaggat ctggtaaagc
    24541 tttatgcggc ccgtcaggag cagaagggct tccagtacgg cagcgatacg gtgtggcaga
    24601 aggagtttga ggagctgttc ccctatgatg aaacagagga tcagttagac gccattgacg
    24661 ctgtaaaaac ggatatggaa agtccaaaga tcatggaccg tctgatctgc ggcgacgtgg
    24721 gatacggaaa aacagaaatc gccctgcggg ccgcgtttaa ggcagtacag gacagcaagc
    24781 aggtggtcta tctggtccct actacgattt tggcccagca gcattacaat acctttgttc
    24841 agaggatgaa agattttcca gttagaatcg agatgatgag ccggttctgt accccggccc
    24901 agcagaagaa aactctggag gggctgcgga aagggctggt ggatatcgta atcggtacgc
    24961 acagggttct ttcaaaagac ctgcagttta aggatctggg acttctcatt attgatgagg
    25021 agcagcgctt tggcgtgacc cacaaggaaa agatcaagca gatgaaggaa aatgtggacg
    25081 taatgaccct gaccgctacg ccgatcccca gaacccttca tatgagtctg gccggaatcc
    25141 gggatatgag tgtgttagag gagccgccgg tggaccgtac tcccattcag acctatgtaa
    25201 tggagtataa tgaggaaatg gtacgggagg ccattaaccg ggaactggcc cggaatggac
    25261 aggtgtatta tgtatacaat cgtgtaacag atattgatga ggtagcagga aagatccagg
    25321 ctatggtgcc ggatgcggtt gtaacctttg cccacggaca gatgaaagaa catgagctgg
    25381 aacggatcat gacggacttt atcaacgggg aaattgatgt gctggtatcc acaacaatta
    25441 ttgaaacagg actggatatc cccaatgcca ataccatgat tatccacgat gcagaccata
    25501 tgggactttc tcagctgtac cagctcaggg gccgtgtggg acgttctaac cgggtttctt
    25561 atgcgtttct catgtataag agagataagc ttttaaagga agaagcagaa aagcgccttc
    25621 aggcgatccg cgagtttacg gagctgggct caggcatcaa gattgccatg cgggatctgg
    25681 aaatccgtgg ggccggaaat gtgctgggcg cagagcagca tggccacatg gaggcagtag
    25741 gctatgatct gtactgcaaa atgcttaatc aggcagtgct tgcattgaaa ggggaagaaa
    25801 cagaggcaga tctttacgaa acttcggtag aatgtgatat tgacgcctac attcctccgt
    25861 cttatattaa aaatgaatat cagaagctgg atatttacaa gaggatttcc accattgaaa
    25921 cggatgagga atatatggat atgcaggatg aactcttaga ccgatttgga gatatcccaa
    25981 agagcgtaga gaatctttta aagattgcca tggtgcgggc tctggcacac agagcctatg
    26041 tgacagaggt atttgtaaac cgccaggaaa tccggcttac catgtatcag aaggcgaaac
    26101 tggatgtgac caggattccg gattttgtgc agtcttataa gggagaatta aagctggtgc
    26161 cgggagaaac gccggtattt cactatgtgg accgaaagaa taaaaacagc gacagcttaa
    26221 tcatgctgga aaaggccaga actatgctgg agcagctggg gaagctgggg cagaaggaac
    26281 cgggcggaaa cggggcagat taaaaagcag gagacaggag gtagcggtta tgccggatac
    26341 agattatgga tatgcattga aaatgggaag aaagcggtat caggatgctt tagcaagagg
    26401 cgagtatcca taccttcccg tgctggataa cattctggcg aatacagata ttgtagcaac
    26461 tgtcaatctg ggggtaatgg atattcctct ggcaaaaatg gtagggacaa agactgcaga
    26521 gcgttccagc tcctttgcca gtaattttat gccccttttg ccggaacggt ccgaatttgg
    26581 agctaaatgg acatcccttt acaagtatca ggtagaggag ggaatccagg aaccggttgt
    26641 tgcctacgaa tttatgaatc agttttacgt tcaggaggga aataagcggg tcagtgtaat
    26701 gaaatatctg ggtgcctaca gcataccggg aagcgtaacg cgcctgatcc ccaaacgtac
    26761 agatgacaag gaaaacaggc tgtattatga atttctggat ttttaccgca tttcttcaaa
    26821 ctgtgatgta tggttcagca aggaaggcag ctataagaag cttttggaac tgatgggaat
    26881 ggagccggat caggtctgga ctcaggagga acgtttattt ttccgctcct cctacgggcg
    26941 ttttgcaaag gcctacagca tggcccatgg ggatcagctt cagctcacag agggagatgc
    27001 gtttttagtc tatatcgaaa tctacggtta cgacagcgta aaaggacaga cagaacggga
    27061 aatgtaccgg gcgctgatga aaatctggga ggagatccag cttgctaacc ggggacggaa
    27121 ggtagagctg atcgaagaac cggaggaaat ggaaacggag cataaaccgt ccatatggaa
    27181 atggcttctt ccggcagatg atatgccgga gcatttgaaa atcggatttg tttatgcgga
    27241 taccattgaa aattccagct ggaattacgg ccatgagctg gggcggcagt atctggagga
    27301 ggcctttgag gggcgtattt ccacggtagt tatcgaaggg gcagatacag aaggggatat
    27361 cgcccgggcc atagaaaaac tggttcaggc cggctgcacc atgatattta caacaaattc
    27421 caagatggta aatcagagcg tgagatccgc cattctgcat cctgaggtaa agatttacaa
    27481 ctgttctgta aatatgtcct attcctctat ctgtacgtat tatgcaagga tgtatgaggc
    27541 aaaattcctt atgggagcta tagcggcagc cgtttccaga gaggataagc tggggtatat
    27601 tgcagatcag cccacctatg ggattcttgc agatatcaac gcttttgcca tgggcgcgcg
    27661 gatgatcaac ccttatgtaa aggtatatct ggaatggcgc aggatcaacc atgaaaagac
    27721 agcggcagaa cggctgagag aacaggggat ccggtacatt tccggaaagg atatgattat
    27781 ccccagacat ccgtccaggg aatacggcct ttatgtacag gaagataatg gagaggtttt
    27841 caatctggct tcgcctatct ggaactgggg gaaattctac gagcagatga tccagctggc
    27901 ctttgaaagc aatgaggagc tggaagccag aaagggcaaa aaagcggtta attactggtg
    27961 gggaatgtcg gcagatgtga tcgacgtaat ctgttccgga aacctgcctc acggtacgct
    28021 gcgcctgatt gaatttctga aaaattccgt tcgttccgga agctttcatc cctttgacgg
    28081 tttcatgtat gaccaggagg gaatcatccg atgcagggag ggagaacgtc tgcgctcaga
    28141 agatattgta accatgaact ggctggctga aaatgtagtg ggacgcgtgc cggatctgga
    28201 agaattgaat gacgaggcaa gagaacggat gcagcttcag ggcatccgtg tagacgagag
    28261 tgtacagacg gagaaatagc ggtatgaata taatgatttt atcagatcag gagtcaaagc
    28321 tcctgtatga atattatgat cctgagagga ttaaggatat tgaccttttg atttcctgcg
    28381 gagatcttcc gccggaatat ctgacttttt ttgccagcgt gtgtcatgcg cctttagttt
    28441 atgtaagggg caaccatgac aataaatacg cctataagcc gccggagggc tgtatctgcg
    28501 ttgaggacga tatttatgtg tataagggag tccggatcct ggggctggga ggttctatgg
    28561 aatatattcc gggctcggcc aaccagtaca cagagcgcca gatgcgggcc cgcgtccgaa
    28621 agctgagatg gaagcttctc tggcacagag gctttgatat ccttgtaacc catgcgccgg
    28681 ccttcggcct gaatgatatg gaggatctgc ctcaccgggg ctttaaagca tttcttaccc
    28741 tgatggataa ataccacccg aaatttttct tccacggcca tgtccatgcc aattacggag
    28801 gaaaatttaa acggaaggat acatatggaa gtacgaccgt ggtgaacgga tatgaatttt
    28861 atgtggtgga gtatccggag ggggagtgag aagtaaattt cgcgcataaa atcccaccat
    28921 ttacaaatac tacatttaca accagtctta aggataaatg gaggtacttt ttcaatgatt
    28981 tcagaaagag tacaaagatg taaaatggag gaaactgcga aatgaaagct acaggaattg
    29041 taagaagaat cgacgat
//
LOCUS       DBMDUH010000012        28524 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_1194_length_28524_cov_6.208282, whole genome shotgun sequence.
ACCESSION   DBMDUH010000012 DBMDUH010000000
VERSION     DBMDUH010000012.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 28524)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 28524)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28524
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1194_length_28524_cov_6.208282"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="ACZPCG_02285"
     CDS             complement(<1..830)
                     /locus_tag="ACZPCG_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HIR1283934.1"
                     /translation="MKLTLNGLKEKEQWEKAGVALPAYDIAAVAEETKKSPVWVHFGA
                     GNIFRIFIGGIADTLIANGEASKGITCVETFDFDVVDKIYAPYDNLVLAVTLKADGST
                     DKRVLGSLTEAIKAQSNDASQWNRLKEIFVNPGLQMISFTITEKGYALKGADGSYFPF
                     IQADIDNGPDKAGSAMAVVCALLYERYKAGKYPLAVVSMDNCSHNGEKLQSSIVTMAK
                     EWSKKGFVEDDFVAYITDEAQVSFPWSMIDKITPRPADSVCQELEKAGIEDMAPVITS
                     "
     gene            complement(833..1906)
                     /gene="uxuA"
                     /locus_tag="ACZPCG_02290"
     CDS             complement(833..1906)
                     /gene="uxuA"
                     /locus_tag="ACZPCG_02290"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HIR1283935.1"
                     /translation="MNMTLRWYGPGYDSVTLEQIRQIPGVKGVITTLMGKMPGEVWEE
                     AEIAELKKTVEDAGLSIMGIESVNVSDDIKIGTEKRDEHIKNYITTLENLGKAGIHMV
                     CYNFMPVFDWTRSDLARKRPDGSTVLAYNQDVIDQIDPEHMRESIEKMSNGFVMPGWE
                     PERLEKLKALFDAYKDVDSEKLFNNLVYFLEAIGPVCEKYDIHMGIHQDDPAWPIFGL
                     PRIVRNKETIERLLKAVDKPYNGLTLCTGSLGSNPENDICEIIRMAKGRIPFAHVRNV
                     KYNSDRDFEEAAHLSSDGSKDMYEIMKTLYDTGFDGCIRPDHGRMIWGEVAMPGYGLY
                     DRALGACYLQGLWEAIDKNAKRG"
     gene            2166..3188
                     /locus_tag="ACZPCG_02295"
     CDS             2166..3188
                     /locus_tag="ACZPCG_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HIR1283936.1"
                     /translation="MKAVQIVKPEDLRVIDIEKPVIDEKNNVLVKMTAAGICGSDMGI
                     YHGTNAAATYPRIIGHEMVGRVDQVGSNVKNLKVGDRVIINQVTSCGHCYPCRKGRGN
                     VCDNLAIRGVHIDGGYREFIAVPESDCYVLPDSLSDTDAIMIEPTTIGIQSCTRAQLE
                     KDDMLLIYGSGALGSTILKVARLLCDHIIVADIMDDKLELARQNGAKYTINPLKEDLT
                     EKIKEYTEGHGSTVSIDSACTKDSLITLLKATGNAGRVITMGFSVAPTEVNQFLITSK
                     ELDVRGSRLQNHMFGKAIELINEGKLDLNGTVSHTFPLIRAQEAFDFNDSHDPSIRKI
                     ALTFDF"
     gene            3216..3896
                     /locus_tag="ACZPCG_02300"
     CDS             3216..3896
                     /locus_tag="ACZPCG_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1283937.1"
                     /translation="MKILERQPSEKARSYAIRVLLYNITALELPPGSSVSENEVSSVM
                     NISRTPVREALIELSRMDLVDILPKRGSFISKIDYGLIEESRFMRLVLETAIVKLACE
                     QGISSYYMELLEKNLRQESSLSNTQDFSVQLELDNHFHQLLFESVGKSRTFDIIYSQM
                     IHFDRLRILSLKSWQDARVVKDHQNILYAIRQKDSELAEMLITRHLTRFQVEKAELTK
                     RYPDYFTL"
     gene            complement(3947..4975)
                     /locus_tag="ACZPCG_02305"
     CDS             complement(3947..4975)
                     /locus_tag="ACZPCG_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008719606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HIR1283938.1"
                     /translation="MKGIKLEEPRNVDCVELEKPVPGPGEALIRIVTAGICGSDIGAF
                     RGTNTLVSYPRIIGHELAGIVEEIPADNPKGLKPGDKVIVDPYIYCGECYPCSIGRTN
                     CCTSLKVLGVHIDGGMAEYFCHPADMLVKMPEDMDWTDAAMAEPLTISLHGIHRGGLK
                     AGEYCAIIGAGPIGLLAGMIAQAYGAHAILLDLVQERLDFAKELGIEYVINSGEEDAA
                     ARVSEITGGMMAQQVMECSGSNAAIRSSLDLVSNAGRITLTGWPPRETSIPTDIITRK
                     EIDIRGARTSAGEFEEAIELIYTKKVDVAKILTKTISLDEAPETIRDIEKNPGNYMKV
                     VVKVDKTV"
     gene            complement(4991..5443)
                     /locus_tag="ACZPCG_02310"
     CDS             complement(4991..5443)
                     /locus_tag="ACZPCG_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008719608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NanQ anomerase/TabA/YiaL family protein"
                     /protein_id="HIR1283939.1"
                     /translation="MFFSNISIAEKYNYLEEKFTCAYKWLAETDINALPAGSYPIMGD
                     TVVANIQEYTTIPADTGFFETHEKYFDIQYVAKGKEQFGICKRDGLKVKERIEENDLI
                     FYEEPELSGTVLLEEGDMIIVAPEDAHKPRCQAGGPCFVKKVVVKVAL"
     gene            complement(5461..6510)
                     /gene="yiaK"
                     /locus_tag="ACZPCG_02315"
     CDS             complement(5461..6510)
                     /gene="yiaK"
                     /locus_tag="ACZPCG_02315"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HIR1283940.1"
                     /translation="MGVRVPYDKLKQDVKQIFLNLGLTEEKAEICTAVHIQSSADGVE
                     SHGLNRIPRFVEYVQKGWVNIKGEPQLAGAKGAVENYDGQLGIGITNALFCADRAVEL
                     AKKHGIGCVALKNTTHWMRGATYVWKMAEAGFMGMSWINAESCMPLWGSDKRSVGNNP
                     FCMAIPREDGPLILDMAMSLYAYGKLGVYRLAGKQLPYPGGFDKDGNLTCDPGAIEET
                     MRGLPMGYWKGSGMALALDMAAALMANGMSGTDMDIQAKGSCTNCCQIFIAYDPYLFG
                     SKDEIQDMLNRRIDAVKKAHPEREGGSVSYPGERTLAVREESMKKGIHVDEAIWKQLC
                     SLASGDMSTTDIASK"
     gene            complement(6525..7574)
                     /gene="yiaK"
                     /locus_tag="ACZPCG_02320"
     CDS             complement(6525..7574)
                     /gene="yiaK"
                     /locus_tag="ACZPCG_02320"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HIR1283941.1"
                     /translation="MSIRVAYEEVKKNVKQAFLNLGLTEEQAETCATIHTQSSADGVE
                     SHGLNRIPRFVEYVQKGWINLEGKPQLVGAKGAVENYDGQLGIGITNALFCAERAAEL
                     AKEHGIGCVALKNTTHWMRGGTYAWKMAEAGFMGMSWINTESCMPLWGSDEPSVGNNP
                     FCMAIPREDGPIVLDMAMSQYAYGKLGVYRLAGKQLPYPGGFDKDGNLTSDPGAIEDS
                     KRILPTGYWKGSGMALALDMAAALMANGKCGTDMDNENRGSCTGCCQIFIAYDPYLFG
                     SKEEIQAMLNKRVDAANAAHPEHEGGHVSCPGQRTISTREKSMKDGVSVDEAIWEQIC
                     ALAAGNMETKDIASK"
     gene            complement(7594..8385)
                     /locus_tag="ACZPCG_02325"
     CDS             complement(7594..8385)
                     /locus_tag="ACZPCG_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1283942.1"
                     /translation="MDRNKVQEALKPITDGLFGKQRPVRYERPHFKVRPLVRMLQDLT
                     SIPIEDMYAYAFSREPLNGKFDDGQRKSWMKQAVCCGREYAEKICCLYGVRSPEELSA
                     AMGLKVEYPTFPENADRVLFAEFREPGNIKIYMDAVKKAHKFTERPEVKEVLTEQLDI
                     KGLLLAHELFHFVEEKYKDEIFTKQEKIRLWSLGPLHNDSTVIALSEIAAMAFAQAIT
                     GIPYSPYVMDVFLVYGYSPEEASGLYEEIMEYAGKIPCCEEGEPV"
     gene            complement(8392..10528)
                     /locus_tag="ACZPCG_02330"
                     /pseudo
     CDS             complement(8392..10528)
                     /locus_tag="ACZPCG_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008719614.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
     gene            complement(10655..12040)
                     /locus_tag="ACZPCG_02335"
     CDS             complement(10655..12040)
                     /locus_tag="ACZPCG_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008719615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HIR1283943.1"
                     /translation="MEYIIGIILLFTFFGLAYYCIKGHNLMIGFLIITLIWTILPLGG
                     TLFASQAFLESNPVLQFEGAKGLVGILNKIYQSAPEGWGTTLVNVCWGAWFGRVLMET
                     GIASTLIRKTVELGGDKPLVTITLLNIVTAVIFTAMTGAGPVIAIGVIVLPILMSLGV
                     PKAIAMFSFMGSVAAGIYLNPVNFAQYRAFFIQPDQMPDFTLGWYVSHWGLAALLIML
                     VATTVLSAVYLKSSKTSHAWAAQTRGTADQKNAPFYTLILPIVPVILKIWLDFSVIGG
                     FVIAGFAALFLCGKMKGTFKENCQLVNKLYYDGVVDTAPLVGFLLTLPMFNTVASYAS
                     PYFKVVLGGIMPRSELVVCIAFAALLFMGLFRGPMTLVGCGAATLGVLSNVASFSVPF
                     LYAVFAIPTITVNIGCCITQSWVAWGLAYTKVDSKDFLKLSIPNAYLTGVLQYAAAFI
                     MLAGLGAEWFI"
     gene            complement(12193..12903)
                     /locus_tag="ACZPCG_02340"
     CDS             complement(12193..12903)
                     /locus_tag="ACZPCG_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1283944.1"
                     /translation="MIKAEELLDKIRSQNEVYNALKYQILTLKAEPGEMLSENIISAQ
                     MNAGRSAVRDALAQLTEEGYVVVYPQKGTAVSMIDPERIKQSVYTHRVLEQSMIEEIC
                     RSDISEEQLKLLEDVLDEQSIKKEEIADLLILEQQLSYALSVLCDKKHIWEMFRIMDC
                     DMLRANYLYYSTYGFQAERYYGPSGWEHMQIEGRMLLDHLKRKDTEAAALICSSHFDR
                     ILLNTNSIQGFYPQFFSG"
     gene            13227..13991
                     /locus_tag="ACZPCG_02345"
     CDS             13227..13991
                     /locus_tag="ACZPCG_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1283945.1"
                     /translation="MTLALFQNQTLPARRPSEDARQYAYRIIRKFILELHLLPGQKMN
                     EADLASSLNMSRTPIHDSFFKLSRENLVDVIPKRGAFVSKIDQKRIDDSVWIHQKLGS
                     AMIHSIYIRKVPKSELELLYCPIQQMNSYMLNGNSAQTVQLLSEYYHQLYMLAGNLDL
                     VWSSLQKTDTDLRRLLSLAVSSPNVLEGLMQELTNLTDALINRDYDNAGLIYDHHFSR
                     IRMLTAPLRTHTPQYFTEPVLPCAASASPQSSDLSS"
     gene            14011..14790
                     /locus_tag="ACZPCG_02350"
     CDS             14011..14790
                     /locus_tag="ACZPCG_02350"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008719627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR1283946.1"
                     /translation="MTDYHALFSLKGKTALITGGTGGLGSAIARAFLQQGAQVAVCGT
                     RPEKAAPIEELAKSLDRPFLSLSCDLTDAESTNQMLNSIEHTFGTVDILVNSAGINRL
                     IPAEEYDDQTFQTVMDINIASLHRITREVGKRFMIPQKQGRILNLSSVKSLIGTKQDY
                     IAYCTSKGAVNMYTRQLACEWGKYGITCNAIAPTFVYTPINAFQLDDPVFYKTLTERI
                     PLGRIGKEQDIASAALYLCSDAASFVTGQILCVDGGLTAMQ"
     gene            14855..15355
                     /locus_tag="ACZPCG_02355"
     CDS             14855..15355
                     /locus_tag="ACZPCG_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NanQ anomerase/TabA/YiaL family protein"
                     /protein_id="HIR1283947.1"
                     /translation="MIYGNINSKHFEQELAILPRPLQNAIRFLKDNDLANHEPGRFDL
                     DFEGVPVILQVLDLTTAPRETLRPEIHRKNIDVQFLASGGPEEAGFYWDNGTSQIDED
                     LLDTPRDILFYHNNPAVPEGRISLDIGTYAVYFPWDVHIPAIQTGTASAPIRKIVIKV
                     PVEACV"
     gene            15374..16201
                     /locus_tag="ACZPCG_02360"
     CDS             15374..16201
                     /locus_tag="ACZPCG_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HIR1283948.1"
                     /translation="MTIDRHLKHLAQKDPENRQKALLDVLIQEGLEFSLQEQEPSIQN
                     PRGIRNYLLTPWSPEPSLLFCAHYDAVPGTFGANDNAAAVCILIQLAQTLKKEHIPAR
                     FAFFDGEEAGNMGSKFYVSSLDRTSLTGVINLDVCGYGDTIAICGKGHEKKPVMRPFC
                     QKKLLESYRGQLLKYLPKSDDSSFRGLHLPVLSLAAVPLWDIQYLKALASYGEGLLGR
                     PPEFEMILGQMEVISTMHGGYRDTPEWVSSDTMNQIYHYLLDGMHSPPVSSKRFIFF"
     gene            complement(16273..17184)
                     /locus_tag="ACZPCG_02365"
     CDS             complement(16273..17184)
                     /locus_tag="ACZPCG_02365"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002603894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HIR1283949.1"
                     /translation="MRIAVIGAGAMGSIYGGHLSQHNEVILVDTNQQVVEQINKNGLL
                     VDENGTSVSYQPKALCDTSEEKPVDLVILFVKALFSRAALENNSALIGPDTRLMTLQN
                     GAGHEDLLKEFVSEDRVIIGTTEDNGAVLGLGHVRRGGTGVTNVGMLAEDQDGFLTRL
                     KENFDSCGFEVRIHENIQYLIWDKLFTNVSLSALTGVLQVDMGYIAADEFAWKLCCQL
                     IHETILTAAAAGLEFDEEAVREKVRKTSLNNPQGCTSIRADLRDGRRTEVDTISGSVV
                     RAAAKYGVDVPGHTFVVNLVHAMEGRK"
     gene            complement(17240..17923)
                     /locus_tag="ACZPCG_02370"
     CDS             complement(17240..17923)
                     /locus_tag="ACZPCG_02370"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004070086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR1283950.1"
                     /translation="MCVYEMGNLRVIDLSKILDPATETRRCHLFRFNTGGPIPDFHTH
                     MDLMSHLGTHCECPYHHDDNWPSAADLPLTTFLGRAVYVDFKDTVAKRGHITAADLDR
                     EAGKVKEGDIVIIDSSYKLVPFTPDTNTDKDERLLVGAESAQWFKDHKVKAVGFGDGV
                     SIENCNEDVKPFHDILMAENIVFLEVLKNLEQLKKDVFFMSYSPLPIKGLDSSPVRAY
                     AIEGLAEFS"
     gene            18211..18858
                     /locus_tag="ACZPCG_02375"
     CDS             18211..18858
                     /locus_tag="ACZPCG_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1283951.1"
                     /translation="MKEKSFLQQQAYDTIKSSILSGEMEPNRLYSETKLSAQIGISRT
                     PVREALQCLSQDGYITVIPSKGFMIRQLNDKDMKDSIEIRCAIEGFCTNMVASQIDTE
                     KGRQLLHSLEQTLSDMEESLENGVQLEHFIDSDHQFHLLLVGYMENDEFNQIFQRLLY
                     LIRLTSQDALAVEGRIQGTMEEHRNYLHALRNGDGPRAYQLMMEHLLMPLKMHEK"
     gene            complement(18865..19581)
                     /locus_tag="ACZPCG_02380"
     CDS             complement(18865..19581)
                     /locus_tag="ACZPCG_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HIR1283952.1"
                     /translation="MNIYFDMHTHTVASGHAFSTFKENVEEAAGKGLFALGMSDHAPA
                     MPGSAPSIYFSNFKCFRQEVMGVRIFTGVEANIMDFNGNLDLDETVLRKMDYVIASLH
                     IPCIKPGTPKENTDALIGAMKNPYVKIIGHPDDDRYPLEYDRLIPAALEHKVALEVNN
                     SSFNPRSGRQNAYKNVMKWLSIAKEYRLPVILGSDAHIYYDVGDLTEAVKMLEAAGYP
                     QELVLNSSKDGIYYILNEGR"
     gene            complement(19596..20624)
                     /locus_tag="ACZPCG_02385"
     CDS             complement(19596..20624)
                     /locus_tag="ACZPCG_02385"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1283953.1"
                     /translation="MSLHIQQVTKTFSKTQALKEINLDIRDSEFVAILGPSGCGKTTL
                     LRIVGGFIEPTSGIVRLNDQIYSGQGHMVPVEKRDLGMVFQSFALWPHMTVRQHIEFP
                     LKSPRHSGMSSEEKKAAIEQAVSCTGLKPYENRLPGELSGGQRQRVALARAIVGKPSI
                     LLMDEPLSALDAELKIDMRREIQNIHKLTGATILYVTHDQSEALAMADRIIIMKDGAV
                     EQIGTPREIYRDPQTVFAATFVSRCNLLKGSWCGDSFHVKDENILLRNPMIPMEFRNK
                     GLYPIRPEQLTIQPEGNGIQGIIVNKQYNGREIHYSVQHRDEVLTIYAGCQAEYNPGD
                     HISFTLSA"
     gene            complement(20560..22305)
                     /locus_tag="ACZPCG_02390"
     CDS             complement(20560..22305)
                     /locus_tag="ACZPCG_02390"
                     /inference="COORDINATES: protein motif:HMM:NF012738.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1283954.1"
                     /translation="METIRKTGASIARIAVYLLAVLVFLLPLIRLLLMGVTVENGYGL
                     DNFAGLLYQERTRKAIVNTIIIAVSSTALAAGCGSGLAFLTAYCNIKRKGVIEFLVLL
                     PFIIPSYIITLSWSSFFSSRGAFNGFLTSIGLPGVDIYTMGGIILVLGFCNIPIVYVN
                     VVHMLRKIPRDMEWAARACGYSQRQAILKINLVSAAPAIAAGSVLAFLSAIDNFSVPA
                     FLGISSGIPVLSTYIYENAIGFGPNAFPLAAALSMILSVIAAAGTMLEGFFVKRGASM
                     DSIKEDYSVRIHLKNPLRLCLEWGTLVLLLILNIIPMAAMTASSFLKAYGLDFVPENL
                     TLKNFAFVFHNRGVISAIHTSLTLAVVTTLVCIVAGTAIAYGKLRKKSRAAALLEKCA
                     SLTYAIPGIVLALSMIFHWVEPIPGVYPGIYGTINILVVAYITRYLVLQIKGSTIALL
                     SVEPAIEEACAASGRGFWIKWIKILIPLVAGPILSGAFMIFVPAFTELTLSSMLASAG
                     TKTIGLTIFNYQQGGDYNLAAAMSVLITILVVAGYCLLNRDHLTSVKQEENGYEPTYT
                     AGYKNIQQNPGPQGN"
     gene            complement(22322..23401)
                     /locus_tag="ACZPCG_02395"
     CDS             complement(22322..23401)
                     /locus_tag="ACZPCG_02395"
                     /inference="COORDINATES: protein motif:HMM:NF024808.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1283955.1"
                     /translation="MKKRFAAVLTAGLAVSSLVGCQNNYSETTASASEQIADNSSEAV
                     LEETSKKVSQDLSGSITLYTSQPEEDIQVMIDGFNKKWPDIQVDVFRSGTEEVVSKVL
                     AEKEAGDVLADVLLVADNVTFETLKEQDLLSAYESPELEGIPEIYIDKDHMYVGTKVI
                     TTGIVYNTELTENIPAGFKDLTNEAYKDDLIMPSPLYSGAAAYNLGVLTRTEGLGWDY
                     YQTLKDNGVVVGKGNGSVQNAVVAGEQACGLLADYMAIRSKNDGAPVEFVYPAEGSPA
                     VTEPIGIVKGTDSEDLAKAFVDFVLSEEGQALTAEIGYTPIKSGVEVPEGFKRIDEIT
                     PLTWDTKELYQNREADKEQFSQMFQ"
     gene            complement(23398..24189)
                     /locus_tag="ACZPCG_02400"
     CDS             complement(23398..24189)
                     /locus_tag="ACZPCG_02400"
                     /inference="COORDINATES: protein motif:HMM:NF013429.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR1283956.1"
                     /translation="MANIYLSSTLLWNGSMEEIFDMVFQSGLDGIELWSQQFFYRKFD
                     IDEFEKLAALYPLKNCIHSQSWDLNISSVNDGIRKQSVTEVKKSVELAYQLGLDEVTV
                     HPGHCTISGIGVSYGAYLKESLREILEYAQKWKIDVSLEIMEKIPREFVTTMDAMKEV
                     CGDMFDQFVYTLDVAHCDSEEEILSILQNYRPQISKIHISNRKGSRFHTPLNEGDYDM
                     KKLLPKLAHYNLPMVIEGLDSSGDFSIAKQNIQFIQHIFNRGKLE"
     gene            24361..25197
                     /locus_tag="ACZPCG_02405"
     CDS             24361..25197
                     /locus_tag="ACZPCG_02405"
                     /inference="COORDINATES: protein motif:HMM:NF013539.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HIR1283957.1"
                     /translation="MLEKIYEEYKERLSKSDHKIFGYLLEHEQELPAITAEDLSRRLN
                     ISTATISRFWSKIGFRNLKDLKHNLYEKQESTPYSRMNAALSQWKECGISPDLLLERL
                     SFQLEKTFQVVKPEQVDEAVKLLISSRQVYIFAPDVSKGLGNILQYRLLRLGILLIPL
                     PGGSQLYDAMVNLKQGDLVLLFGYSRLLTEVQILLSYSEKAGYQTILFTDLLAEEHLS
                     NANLTLYSFRGEPNDYHSVATPLLLIDLLIMKASQSLGGGLEKAKKLQNLREQYAGLI
                     RR"
     gene            complement(25351..26214)
                     /locus_tag="ACZPCG_02410"
     CDS             complement(25351..26214)
                     /locus_tag="ACZPCG_02410"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR1283958.1"
                     /translation="MKKIGFIGVGIMGKSMVRNLRKAGYEVAIYTRTKAKAEDVIAEG
                     AVWCDTAAECAKGRDVVITIVGYPKDVEEVYFGDNGIIANADPGTYVIDMTTTSPRLA
                     VRIWEEAEKAGLHAVDAPVTGGDTGAKAGTLTILAGGKKEDFDACVPVFEAMGKNINY
                     EGKAGNGQHTKMCNQIAIAGALAGACEAMVYAKNTGLDVDTMLKSISTGAAGSAQMNN
                     VASKAAKDDYAPGFFLKHFIKDMGIADEEALAADTRLGVLEDVLGMAKKLESEGMGDL
                     GTQALIKYYKW"
     gene            complement(26217..26807)
                     /locus_tag="ACZPCG_02415"
     CDS             complement(26217..26807)
                     /locus_tag="ACZPCG_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HIR1283959.1"
                     /translation="MGYIFYVMGKSAAGKDTIYKELVRRFPEYGTVRMYTTRPIRDGE
                     QEGVEYYFTDEARLKKLESQGKVIECRTYDTVFGPWSYFTADDGQIDLKQGNYLMIGT
                     LDSYEKMVNYYGNEVMIPLYIYVDDGTRLQRALDREKTQNTPKYAEMCRRFLADEKDF
                     SKERLAACGIDRGYENNDFNTCIQLILKDIEAKGDK"
     gene            complement(26837..28351)
                     /locus_tag="ACZPCG_02420"
     CDS             complement(26837..28351)
                     /locus_tag="ACZPCG_02420"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR1283960.1"
                     /translation="MREEDLLINRLKHYGKTGIYPFHMPGHKRLKWGEEADGRAFYQG
                     MEFPNPFEVDITEIDGFDNLHHPEGILKESMEWAAGLYGADRTRYLVNGSTSGLLAAV
                     CGCVHWGERILIGRNCHKAVYNGIYLHHLQVSYVYPQSTKEWGIQGGILPEDVEKSLK
                     EQPDIRAVLIVSPTYDGIVSDVGEIAKIVHRAGLPLIVDEAHGAHFRYSEIFPQSALE
                     LGADVVIQSVHKTLPSLTQTAVLHMKCNGPDGSAYMDMEAVERYLHIVQSSSPSYVLM
                     ASIENGIFQMEQLRRKDGMRKFADSLLEMRESLSAMKNLRLVGRELKGRYGIFDLDPS
                     KVVISTRYASFDGEGLSELLRKQYHLEMEMCGADYVTAITAVGDSKAGLERLRAALLA
                     IDKEGFPSGKMELQGREGAGSDCVYAIEAKTRCSIREAIDGKTARIPLRESAGKISGE
                     FVYIYPPGIPLIAPGECITEQLLEVILDYIRKGLPVQGLSDQTLETILVSEQAI"
BASE COUNT         7736 a         7338 c         5458 g         7992 t
ORIGIN      
        1 ttggaagtaa taaccggagc catatcctca attccggcct tttccagctc ctggcataca
       61 gaatcagcag gtcttggggt aatcttatca atcatagacc atgggaagga aacctgtgcc
      121 tcatctgtga tgtaggctac aaaatcatcc tctacaaagc cctttttgct ccactctttt
      181 gccatggtta cgatagagct ctgaagcttc tcgccattat gagagcagtt atccatggaa
      241 actactgcca gaggatactt tcctgcctta tagcgctcat aaagcagagc acatacaact
      301 gccatagcag aaccggcttt gtctggtcca ttgtcaatat ctgcctgaat aaacgggaaa
      361 taagagccat ctgctccctt taaagcatat cccttctctg taatggtaaa tgaaatcatc
      421 tgcaagccag gatttacaaa aatttccttc agacggttcc actgggatgc gtcgttagac
      481 tgtgccttga ttgcctctgt cagagagccc agcactctct tatcagttga accgtcagcc
      541 tttaaggtaa cggccagaac cagattgtca tagggtgcat aaatcttgtc aacaacatca
      601 aaatcgaaag tttcaacaca ggtaattccc tttgaagctt cgccatttgc aattaaagta
      661 tctgcaatgc cgccgataaa gatacggaag atatttccgg ctccaaaatg aacccagacc
      721 ggagacttct ttgtttcttc cgcaactgct gcaatgtcat atgcaggaag agcgactccc
      781 gctttctccc actgttcttt ttcttttaag ccgtttaatg ttaatttcat tcttaacccc
      841 tctttgcgtt cttgtcaatt gcttcccaaa gaccctgaag atagcaggct cccagcgctc
      901 tgtcatacag accatatccc ggcatagcaa cttcgcccca gatcattctt ccgtggtcag
      961 gacggatgca gccgtcaaat ccggtgtcgt ataaggtctt catgatctca tacatatcct
     1021 tagagccgtc agaggacagg tgagcagcct cctcgaaatc tctgtcgctg ttatacttta
     1081 catttcttac atgggcaaat gggattctgc cctttgccat acggataatc tcacagatat
     1141 cattttcagg attggaaccc agggaacctg tacacagagt taaaccgttg tatggcttat
     1201 ctactgcctt taacagacgc tcaatggttt ccttatttct cacgattctt ggaagtccga
     1261 aaataggcca tgcgggatca tcctgatgaa tacccatatg gatatcgtat ttctcacata
     1321 cagggccgat tgcctccagg aagtatacca gattgttgaa cagtttctct gaatctacat
     1381 ctttatatgc atcaaataat gctttcagct tttcaagacg ctctggctcc cagcctggca
     1441 tcacaaagcc attggacatc ttttcaatag actctctcat atgctcagga tctatctgat
     1501 caattacgtc ctgattatat gcgagaactg tagagccatc cggtctcttt cttgcaagat
     1561 cagaacgggt ccagtcaaat acaggcataa agttgtagca taccatgtga atgcctgctt
     1621 ttcccaggtt ctcaagggtt gtaatatagt tcttaatatg ctcgtcacgc ttttcagttc
     1681 cgatcttaat atcatcggaa acgttcacgg actcgatacc cataatagac aggcctgcat
     1741 cctcaacagt cttctttaac tctgcgatct ctgcctcttc ccatacctct ccaggcatct
     1801 ttcccataag agttgtgata actcccttta ctcccggaat ctggcggatc tgctccagag
     1861 ttacactgtc atagcctggt ccataccaac gtaatgtcat gttcataatg ttctacctcc
     1921 atatgttaaa ttttgttgct tcatcatttt gggcctgata acgctcttac ttgtatggta
     1981 gtataaaatt tcaatttgtt caatatgtaa ttttttataa aaattaacct tttagtttat
     2041 taattctgta catattattt tttatacctc tcttatattg acttttgtcc catagggttt
     2101 tagtataggt agtatgtaag ttgtttaata aaacctgcta tatttcaaaa ggagagtgtg
     2161 tgttaatgaa agctgtacaa atcgtaaagc ctgaagacct gagagtaatc gacattgaaa
     2221 agcctgttat tgacgaaaaa aacaatgttc ttgtaaaaat gaccgctgcc ggcatctgcg
     2281 gttctgacat gggtatctac catggtacaa atgctgcagc aacctatcca cgtatcatcg
     2341 gacacgagat ggtaggccgt gttgaccagg ttggatcaaa tgtcaaaaat ctgaaagtag
     2401 gagaccgtgt aatcatcaat caggtaacca gctgcggcca ctgctatccc tgccgcaaag
     2461 gacgcggaaa tgtatgcgat aatcttgcta tccgtggcgt gcatattgac ggaggctacc
     2521 gcgaatttat agctgttcct gaaagcgact gctatgtttt acccgattcc ctttctgata
     2581 cggacgccat tatgatcgag cctactacca tcgggatcca gtcctgtaca cgggctcagc
     2641 tggaaaaaga tgatatgctt ctgatctatg gatccggagc actgggcagc acgatcttaa
     2701 aggtagcccg tcttctctgc gaccatatca tcgtagctga tatcatggat gacaagctgg
     2761 aacttgccag acagaacggt gctaaataca ccatcaatcc gctgaaagag gatcttacag
     2821 aaaaaataaa agaatataca gaaggccatg gaagcaccgt ttccatcgac tctgcctgca
     2881 ccaaggattc cctcattact cttttaaagg caaccggaaa tgccggaaga gtaattacca
     2941 tgggcttttc agtagctcct accgaggtaa atcagttcct gatcacctcc aaggaactgg
     3001 atgtccgcgg ttccagactt cagaatcata tgttcggcaa agcaattgaa ctgatcaacg
     3061 aaggcaagct tgatctgaac ggtactgtgt cacatacctt ccctctgatc cgcgctcagg
     3121 aagcctttga ctttaatgac agccatgatc cttccatccg caagattgcc cttacctttg
     3181 atttctaatt atcacattta aagaagggat acatcatgaa aattttagaa cggcagcctt
     3241 ctgaaaaggc tcgttcctac gctatacgcg tacttctcta caacatcact gccctggaac
     3301 tgcctccggg aagttccgtc agcgaaaacg aagtatcttc tgtcatgaac atatcccgaa
     3361 cccctgtgcg ggaagctctt atcgagcttt cccgcatgga tcttgtagat attcttccca
     3421 aacggggcag ttttatttcc aaaatcgatt atggattgat tgaagaatct cgttttatgc
     3481 gtctcgtact tgagacagca attgtgaagc ttgcatgcga acagggaatc tcatcctatt
     3541 atatggagct gttggagaaa aatctccgac aggaatcttc tctgtccaat acacaggact
     3601 tctcagtcca gctggagctt gacaatcatt ttcatcagct tctttttgaa tctgtaggaa
     3661 aatccagaac ctttgacatt atctattccc agatgatcca ttttgaccgt cttcgtattc
     3721 tgtctttaaa atcctggcag gatgcgcggg tagtaaaaga ccatcagaat attctgtatg
     3781 ccatccgtca aaaagattct gaattggcgg aaatgcttat cacccgccac ttgacacggt
     3841 ttcaagtaga aaaagcagag ctgaccaaac gttatcctga ttactttacg ctgtaataaa
     3901 acgggcgtgt gaaaaactta cgttttctca cacgcccgtt tctgttttat acggttttat
     3961 ctacttttac aactaccttc atgtagttac ccggattctt ttcaatatcc cgaatcgtct
     4021 ccggtgcctc atccagagaa atagtcttgg ttaaaatctt agccacatct accttctttg
     4081 tgtaaatcag ctcgatcgcc tcttcaaatt ctcctgcact tgtacgtgct ccgcgaatat
     4141 caatttcctt acgcgttatg atatcggtag gaattgaggt ctctcttgga ggccagccgg
     4201 taagagtaat tcttcctgcg ttgctgacaa gatcaaggct ggagcgaatt gctgcattgg
     4261 atccgctgca ctccataacc tgctgagcca tcattccgcc tgtaatttca ctgactcttg
     4321 cagccgcatc ttcttctccg gaattgatta catactcaat acccagctcc tttgcaaaat
     4381 ccagacgctc ctgaacaaga tccagaagaa ttgcatgtgc accgtaagcc tgtgcgatca
     4441 tacctgccag cagaccaata ggtcctgctc cgatgatcgc gcagtactcc cctgccttta
     4501 atccgcctct gtggattccg tgaagagaaa tagtaagagg ttctgccatt gcagcatctg
     4561 tccaatccat atcttccggc atctttacca gcatatctgc cggatggcaa aaatactctg
     4621 ccatgccgcc gtcaatatgt acacccaaaa ccttaaggct tgtacagcag tttgtacgtc
     4681 cgatagagca tggatagcac tctccgcagt aaatgtatgg atcaacaata actttatcgc
     4741 ctggttttaa tcccttagga ttatctgccg gaatttcctc tacgatacca gccagctcat
     4801 gtccaatgat ccttgggtag gataccagtg tattggttcc gcggaaagca cctatatcac
     4861 ttccgcaaat accagctgta acaatacgga tcagcgcctc gcccggtccc ggaacaggct
     4921 tttccagctc tacacaatct acatttctag gttcttccag tttaattccc ttcatttttc
     4981 tcctccatta ttaaagtgcg acttttacga caaccttttt aacaaagcat ggcccgccag
     5041 cctggcacct tggtttatga gcatcttcag gagcaacgat gatcatatct ccctcttcca
     5101 aaagaacggt tccgcttaac tccggctcct catagaagat cagatcattt tcctcaatgc
     5161 gttcctttac tttaaggcca tctctcttgc agataccgaa ctgctctttt ccctttgcaa
     5221 catactgaat atcaaagtat ttttcatggg tttcaaaaaa acctgtatct gccggaattg
     5281 tagtatattc ctgaatgttg gctactactg tatcacccat aataggatag cttcctgcag
     5341 gcagtgcatt gatatctgtt tctgccagcc acttataggc acaggtaaat ttctcttcca
     5401 ggtaattata cttctctgca atacttatat ttgaaaaaaa catagtgctt cctcctgttt
     5461 ttatttgctt gctatatcag tcgtagacat atctcctgac gccagggaac aaagctgctt
     5521 ccaaattgcc tcgtctacat gaatcccctt cttcatgctt tcttcccgta ccgccaaagt
     5581 cctctctccc ggatagctta cgcttccgcc ttcgcgctca ggatgagctt ttttaaccgc
     5641 atcaatcctc cggttcagca tatcctggat ttcatctttg gagccaaaca gatagggatc
     5701 atatgcaata aagatctggc agcagttcgt acagctgcct tttgcctgta tatccatatc
     5761 cgttccgctc attccattgg ccataagcgc tgcagccata tccaaagcaa gagccatacc
     5821 gcttcctttc cagtatccca taggaagccc ccgcatggtt tcctctatgg cgcccggatc
     5881 gcaggtcaga tttccatcct tatcaaatcc tcccggatag ggaagctgct ttcccgccag
     5941 acgataaact cccaactttc cataagcgta cagactcata gccatatcaa ggataagcgg
     6001 gccatcctcc cttggaatcg ccatacagaa cgggttgttt cccacactgc gcttatcgct
     6061 tccccacaat ggcatacagc tttccgcatt aatccagctc atacccataa agcctgcttc
     6121 agccattttc catacatagg ttgcgcctct catccaatga gtagtgtttt tcagggcaac
     6181 acagccaatc ccatgttttt tcgccagctc tacagctctg tcagcacaaa agagcgcatt
     6241 ggtaatgcca atccccagtt gtccgtcata attttccact gcgccctttg cacctgcaag
     6301 ctgaggctcg cctttaatgt taacccatcc cttttgaaca tattccacaa atctgggaat
     6361 tcgattcagt ccatggcttt ctaccccatc agcactggac tgaatatgaa cagctgtaca
     6421 tatctcagcc ttttcttctg taagtcccag attcaggaaa atctgcttta catcctgctt
     6481 taatttgtca taaggaacac gaacccccat atttcctccc ggatttactt acttgcaatg
     6541 tcctttgttt ccatattgcc tgcagccaga gcacagatct gctcccagat tgcctcgtcc
     6601 acagaaactc cgtccttcat gctcttttcg cgagtggaaa tcgttctctg tccaggacag
     6661 cttacatgtc cgccttcatg ctccggatga gctgcgtttg cagcatcaac acgcttgttc
     6721 agcatagcct gaatttcttc tttggaacca aacagataag gatcatatgc aataaagatc
     6781 tggcagcagc ctgtgcagct tccacgattc tcattgtcca tatctgttcc gcatttgccg
     6841 tttgccataa gagcagcagc catatccaat gcaagagcca tgccgcttcc cttccagtat
     6901 ccggtaggaa gaattctctt actgtcctca attgctcccg gatcgctggt cagatttccg
     6961 tccttatcaa agccgcccgg ataaggaagc tgctttcctg ccagacggta tacacccagc
     7021 tttccataag catactggct catcgccata tcgagcacaa tagggccatc ctctcttgga
     7081 attgccatac agaatggatt gtttccaacg cttggctcgt cgcttcccca cagaggcata
     7141 cagctttctg tgttgatcca gctcataccc ataaagcctg cctcagccat cttccatgca
     7201 taggtgccgc ctctcatcca gtgcgtcgta ttttttaaag caacacagcc aattccatgc
     7261 tctttcgcca gctctgcagc ccgctctgca cagaaaagag catttgtaat accgatgccc
     7321 agctgaccgt cataattctc cacagcgcct ttggctccta caagctgagg ctttccttcc
     7381 agattgatcc atcccttctg aacatattcc acaaatctgg gaatacggtt cagtccatgg
     7441 ctctcaacgc cgtctgcact ggactgggta tgaattgtag cacaggtctc ggcctgctct
     7501 tctgtaaggc ccagatttaa gaatgcctgt tttacgtttt tctttacttc ctcataagca
     7561 acgcgaatac tcatatcgtt tctcctttat tttttataca ggctcccctt cttcacagca
     7621 gggaattttg cctgcgtatt ccattatctc ttcatataaa ccgctggctt cctccggcga
     7681 atagccatat accagaaaca catccatcac ataaggggag tatggaattc ccgtgatagc
     7741 ctgggcaaag gccattgcgg caatttcact taaggcaatc accgtggagt cattatgcag
     7801 aggtcccaat gaccagagcc ggatcttttc ctgttttgta aaaatctcat ccttatattt
     7861 ttcttctaca aaatgaaaca gctcatgagc cagcaaaagg cctttgatat ccagctgttc
     7921 agttaaaacc tctttcactt caggacgctc tgtaaatttg tgcgccttct tcacagcatc
     7981 catgtaaatc tttatattgc ccggttcccg gaattctgca aacagaaccc tgtctgcatt
     8041 ttccggaaac gtaggatact ctaccttcag ccccatagct gccgataatt cctcaggact
     8101 tcggacaccg taaaggcagc aaatcttctc agcatactct ctgccgcagc aaacagcctg
     8161 cttcatccag ctttttcgct gcccgtcatc aaactttcca ttcagcggct ctctggaaaa
     8221 agcatatgca tacatatcct ctatcggaat ggatgtcaga tcctgaagca tacggaccag
     8281 aggacgtacc ttaaaatgag gacgttcgta tcttaccggt ctctgctttc caaaaagtcc
     8341 gtctgtaatg ggctttaaag cttcctgaac cttatttctg tccataactg cttatatctc
     8401 cgtaactgaa cctacgataa taatctcctc atcaggctct ctgtctccca gatccagatc
     8461 catcagcagg aagatctgct ccagatcaat tgcgctgtaa tcgcctactc ttggacctac
     8521 gcatacaaag aaatcaggac gaagaatgga gtctgtctta aatacggcag cctctttcca
     8581 gattgtttcc actacttctt tgtaatcgcg gaccggacat tttacaacca taccgttgga
     8641 gcactgaacc ttgataaagc ccttcttatc aacaatacgc agaggattct ttccctctct
     8701 ctccctggta tacatagaac ttgtccgtct tctctgccag gtgaatctgg cttacctttg
     8761 atccaaaatc ctgtgtagcc agttccagag cctctgcctc atcacactct ttcagaagat
     8821 ccgttgtctt tacttctgta gatcctgttg cgattgctgt taccttaccg gtctgactgt
     8881 cgatttcaat atgaacctct accgtatcag gtgatgcgcc ggagcttacc gctgcatcag
     8941 ccgcctcttt cttaatctcg cggatatcat cctgagtcgg attggggatc actcgctcta
     9001 ctacgtcacg gaccatggaa agcgcaacgc cgatagagga aataacctca gcattctccg
     9061 gaatgctgta ctgcagtccc atctttcctg cacagtaagg tacaagagac gctgctccgc
     9121 ctccgcagcc taccagcttc atgctgtcat gatccagctg atacttctca gccagagcgt
     9181 tgatgcaggc atttaccttc tcataatcct tatccagaat ctgtgttgca agctgctcta
     9241 cagtgattcc aagcttatct gctacaggct gcattgcttt gcgggctgca ttggcatttc
     9301 cataagcaaa atacttctta tcgatcaatc ccagaacatt ggctgcacag gtattggtaa
     9361 agcagatacg ctttccgttc tttaaacgga ttgtaacgta atcagcagga tcgcttggtt
     9421 tcggtcttac catctcgatc tgcggatcaa caatctcctc ttcaggagta aaacatgcgt
     9481 attcgcagcc tgcgatatga gcagaacgag gacctacgtc tactacttcc ttgtcattga
     9541 tacgaaccat agaaccgccg gcacatccaa gaatgcggac atccaaagaa ctgatatatg
     9601 tatcatggcc tccgatctgg gcatagtcta caccgggacg gccattcttg attacaccga
     9661 tattggttgt tgttccacct acctcaaagt aaatggcatt ggatgctctt aagtacatca
     9721 gagatcccat aacagatgcg gcggggccgg ataatgcagt taaaatcgga cgcttacgca
     9781 tctcgttaat ctccataaca ccgccgtcgc ctctcatgat catcagtgga acctgaacgc
     9841 ctgcttctct tacagaagac tctgttgcgt ttgcagttgc catcatctta ggaaggatgg
     9901 acgcgttaat tgcagctgta cgggtacgtc ttgtaagtcc atacagtttt gtaatatcag
     9961 aagccatact aaccggcaca tttttctttt ctgcacagtc atggatcatc atttcctcat
    10021 ccatgctgtc tacgccaaag gccatagaag caacaattac ctgtgcgccc tggctgatca
    10081 agtcatcaat atttttattg atcacttcct ccgtcagcat ctttttctta atgaaggtat
    10141 taaatacctt gatcattctg ccaacttttt catccaatac aatatccttc aaagccagct
    10201 ggcgctttgc aagaaatcct tccagaccgc ctcccgctac gccaactaca cctacatttg
    10261 ccacgtcgcc ctcaataaag gcattggtag cctgagtagt agaatgggct acgaacacca
    10321 catcctccgg tgcaatgtca ttttcccgca tacaattctg aaaactctga acaacaccgg
    10381 cagcaacgcc gtttttgtcg tcgtgggtag tttttacttc attttttcca ataatttcat
    10441 gagtctcatt gtccattgct acgcattttg tgtaggttcc acctacgtca atacccatgc
    10501 ggacagctct gtgcttacgt tctgccatac tttccatatc ctttcaactt gattattcct
    10561 ttggattttc agaaacatac taaaggggct gcgggcgcat ccagagcgcc cgcagaataa
    10621 aaatatacat tgtttgagag attttatttt aagatcagat aaaccattca gcgccaagtc
    10681 cggcaagcat gataaatgct gccgcatact gaaggacacc tgtcaaatat gcatttggaa
    10741 ttgataattt caagaagtcc ttagaatcaa ccttggtgta agccaatccc catgcaaccc
    10801 aggactgtgt aatacagcat ccgatgttaa ccgtgattgt aggaattgca aatactgcat
    10861 ataagaatgg aactgagaag gaagccacat tggaaagaac gcctaatgta gcggcaccgc
    10921 agcctaccag agtcataggg ccacggaaca gtcccataaa cagcagggct gcaaatgcaa
    10981 tacatacaac aagctcgctt ctgggcatga ttccgccaag aacgacctta aaataaggag
    11041 atgcatagga tgctactgtg ttgaacatcg gcagtgtaag aaggaagcct accaatggag
    11101 cggtgtctac aacgccgtca taatacagct tatttaccag ctggcaattc tccttaaatg
    11161 tacccttcat ctttccgcac aggaacagag ctgcaaaacc ggcaattacg aagccgccga
    11221 taacggaaaa atcaagccag atctttaaga taacaggaac aatcggaagg atcagagtat
    11281 agaacggagc gttcttctga tctgcagtgc ctcttgtctg ggcagcccat gcgtggctgg
    11341 tctttgagga tttaaggtaa actgcggaaa gaacagttgt agcaacaagc atgatcagca
    11401 gagctgcaag cccccagtgg cttacatacc atcccagagt aaagtcaggc atctgatccg
    11461 gctggataaa aaaagcgcgg tactgtgcaa agttaacagg atttaaataa atacctgctg
    11521 caacagaacc cataaagctg aacatggcaa ttgctttcgg aactcctaaa gacataagaa
    11581 taggaagaac gataacgccg attgcgataa cagggccagc tcctgtcata gccgtaaaga
    11641 taacagctgt aacaatattc agcagagtga tagtaaccag aggcttgtct ccgcctaatt
    11701 caacagtctt acgaatcagg gtagacgcaa ttccggtctc catcagaaca cgaccaaacc
    11761 atgcacccca gcatacgttt accagtgtag ttccccagcc ttccggagcg ctctggtaaa
    11821 tcttgttcag gataccaacc agtccctttg caccttcaaa ctgaagaacc ggattggact
    11881 ccaggaatgc ctggcttgca aataatgtgc ctcccagagg aaggattgtc cagataagtg
    11941 tgatgatcaa gaatccgatc atcaggttgt ggcctttaat acagtaatat gccaggccaa
    12001 aaaacgtaaa caataaaatt ataccgatga tatattccat ggtatttcct ccatttcttt
    12061 tttccctacg tcatacacat tgcatctctc tcaacaatga tatccccatt tcatctccct
    12121 tcatgataaa ttagtatatt ttctaaccgg cgtcaaaacg tcctgacagt tttttgctgt
    12181 cagccatttt tgtcagccgg aaaagaactg tggataaaat ccctgaatgg aattcgtatt
    12241 aagaagaatt ctgtcaaaat ggctggaaca gatcaaagct gccgcttccg tatccttcct
    12301 tttcagatgg tccagaagca tccttccttc tatctgcata tgctcccagc cagagggacc
    12361 ataataccgc tccgcctgaa aaccatatgt actgtaatac agataattcg ctctcagcat
    12421 gtcacaatcc ataatccgga acatttccca aatgtgcttc ttatcgcaga gaaccgaaag
    12481 cgcatagctt aactgctgtt ccagaatcaa aagatcagcg atctcttcct tttttatact
    12541 ttgctcatcc agtacatcct caagaagctt cagctgttct tctgagatat cgcttctgca
    12601 tatctcctca atcatggact gttcgagtac tctgtgagta tacacagact gtttgatccg
    12661 ttccggatca atcatagaaa cagccgttcc cttctgggga taaacaacaa catagccttc
    12721 ctctgtcaac tgggcaagag catccctcac tgccgacctc ccggcattca tctgagcaga
    12781 tatgatgttc tcactgagca tctctcccgg ctctgccttt aaggtaagga tctgatattt
    12841 cagagcattg taaacctcgt tctgagatct gatcttatcc agaagctctt ctgccttaat
    12901 catccgaccc cgtcctcctt tgtcctgttt aatcattttg catacaagct ttaaattgca
    12961 tacaagtttt actcctgatt gcatatcagt ttcaaaaaaa acagcctatc aaatcaggcg
    13021 tgtgtttgtc gctatgtata aaatatcata tttttttaac aaatacaata tcttttttta
    13081 ttttttgtta ataaattttg tttttgtttg ttatccgtat tgctttttcc acaagtaatg
    13141 ataaaataag attagagctt tattcgattt gaaattgcat acaagtttgc agttaactat
    13201 atttttaaga aagaacggtg acacacatga cactggcctt atttcaaaac cagacgcttc
    13261 ctgcccgcag gccttccgag gatgcgcggc agtatgccta ccgtattata cgaaaattca
    13321 tccttgagct tcatcttctt cccggccaga agatgaatga agcggatctt gcctcatctc
    13381 tgaatatgag ccggactcca atccacgatt cgtttttcaa attatccaga gaaaatctgg
    13441 tagacgtcat accaaagcgg ggcgcatttg tctccaaaat cgaccagaaa cgcattgatg
    13501 actctgtatg gattcatcaa aagctgggaa gcgccatgat ccattccatt tatataagaa
    13561 aggttcccaa atccgagctg gagcttctgt actgtcccat ccagcagatg aacagctata
    13621 tgcttaacgg aaattcagct cagaccgttc agcttctgtc ggaatactac catcagcttt
    13681 atatgctggc cggaaatctg gatctggtat ggtcttctct tcagaaaacc gatacggatt
    13741 tgagacgcct gctgtctctg gctgtaagca gccccaatgt actggaaggc ttgatgcagg
    13801 agctgacgaa tctgacagac gcccttataa accgtgatta tgacaatgcg gggctgatct
    13861 atgaccacca tttctccaga atccggatgc ttacagcccc tctcagaacc catacccccc
    13921 agtactttac agaacctgtg ctgccctgtg ctgccagcgc ttctccacag agttctgatt
    13981 tatcctctta aagaaaggcg gtatcacatt atgactgact atcatgcatt attttctctc
    14041 aaaggaaaaa cagcactgat caccggagga accggcggtt tgggaagtgc gattgccaga
    14101 gcatttcttc agcagggcgc tcaggtcgct gtctgcggta cccgtccgga aaaagcggct
    14161 cccatagaag aactggctaa aagcctggac cgtccatttt tatccctctc ctgcgatctg
    14221 actgatgcag aaagcacaaa ccagatgctc aactccatag aacacacctt cggcactgta
    14281 gatatccttg taaacagtgc cggaatcaac cgtctgatcc ctgctgagga atatgacgac
    14341 cagaccttcc agacggttat ggatataaac atcgcctctc tccaccggat tacccgtgaa
    14401 gtaggaaaac ggtttatgat ccctcaaaaa cagggacgga ttctgaatct ttcctctgta
    14461 aaaagcctga tcggaacaaa gcaggactac attgcctact gtacaagtaa aggcgctgtc
    14521 aatatgtata cccgccagct ggcctgtgaa tggggaaaat acggcatcac ctgcaacgcc
    14581 attgctccca cttttgtata cactcccatt aatgcattcc agctggatga tccggtattt
    14641 tacaaaacat taaccgaacg tattcccctt ggaagaatcg gcaaagagca ggatattgct
    14701 tctgctgccc tttatctgtg cagcgacgcc gcctcttttg taaccggaca gattctctgc
    14761 gtagacggag gtctgactgc tatgcagtaa ttctgcacac gacttactgc ctttcaatat
    14821 ccatcaaaaa catttaccat taggaggtat cattatgatc tacggaaaca tcaacagcaa
    14881 acactttgaa caggaactgg caatccttcc cagaccactg cagaacgcaa tccgcttttt
    14941 aaaggataac gacctggcca atcacgaacc aggaagattt gatctggatt ttgaaggtgt
    15001 ccctgttatt ttacaggttc tggatctgac taccgctcca agagaaacac ttcgtccgga
    15061 aattcaccgc aaaaatatag acgtacagtt ccttgcctcc ggcggaccgg aagaagccgg
    15121 attttactgg gataacggta caagccagat agatgaagat ttgctggata cccctcgtga
    15181 tatcctcttc tatcacaata accctgctgt accggaaggc cgtatctctt tagatatagg
    15241 tacctacgct gtatacttcc catgggatgt acacattcct gcaatccaga ccggaacagc
    15301 cagcgctcct atccgtaaaa tcgttataaa ggttcctgtg gaagcctgcg tataacagca
    15361 aggagactat ttcatgacca tcgaccgaca cttaaaacac ctggcacaga aagatcctga
    15421 aaaccggcaa aaagcccttc ttgatgttct catccaggag gggctggagt ttagccttca
    15481 ggaacaggaa ccgtccattc agaaccctcg cggaatacgt aactaccttt taactccctg
    15541 gagtcctgag ccctctctcc tcttctgtgc tcactacgat gctgtacccg gaacatttgg
    15601 agctaatgac aatgctgcag cagtctgtat cctgatccag ctggcacaga ctctgaaaaa
    15661 agagcatatt ccggcccgtt ttgccttttt tgatggcgaa gaagcaggta atatgggcag
    15721 caaattttac gtttcttccc tggaccgtac ttccctgaca ggtgtcatca atctggatgt
    15781 atgcggctat ggagatacca ttgccatctg tggaaaggga catgaaaaaa agccggttat
    15841 gcgccccttc tgtcagaaaa agcttcttga atcctacaga ggacagcttt taaaatacct
    15901 ccccaaaagc gatgacagct ctttcagagg acttcatctt cccgtattga gtctggccgc
    15961 tgttcccctg tgggatatac agtatttaaa agctctggcc tcttatggag agggtctttt
    16021 aggccgtcct ccggaatttg agatgatcct cggacaaatg gaggtaatct ctaccatgca
    16081 cggcggatat cgggatacac cggaatgggt aagctctgat accatgaatc agatatacca
    16141 ctatcttctt gatggcatgc acagccctcc tgtttcatct aaaagattca tcttctttta
    16201 attcagaatc caggggggca ggcagctgca aatgcagctg cctgcccccc tgactgtata
    16261 cacagcccca aattactttc tgccttccat tgcatgaacc agatttacta caaaggtatg
    16321 tcccggcacg tccactccat atttagcagc agcccgtaca acagaaccac tgatggtatc
    16381 tacctcagtt cgtcttccat ctcttaaatc tgcccggata gacgtacatc cctggggatt
    16441 atttaatgaa gtttttctta ccttttccct tacagcttct tcatcaaatt caagacctgc
    16501 tgccgcagcc gtaagaattg tttcatgaat cagctggcag cagagcttcc atgcaaattc
    16561 atctgccgca atatacccca tatctacctg aagaacacct gtcagagcac tcaaagacac
    16621 attggtaaac agcttatccc agatcagata ctgaatattt tcatgaatcc gaacctcaaa
    16681 gccgcagctg tcaaaatttt ctttcagacg ggtcaaaaat ccatcctgat cctctgccag
    16741 cattcccaca ttggtaacac ctgttccgcc tctgcgcaca tgacccagac ctaaaacagc
    16801 accgttatcc tctgtcgttc caataatcac ccggtcttca cttacaaact ctttcagcag
    16861 atcctcgtga ccagctccat tctgaagggt catcaggcgg gtatccggac cgataagagc
    16921 cgaattgttt tccagggctg ctctggaaaa taatgccttt acaaagagga tcaccagatc
    16981 aacaggcttt tcttctgatg tatcacaaag agccttgggc tgataggaga cagaagttcc
    17041 gttttcatca accagaagac catttttatt aatctgctct actacctgct gattggtatc
    17101 taccaaaata acttcattat gctgcgaaag atggcctcca taaatagatc ccatagctcc
    17161 cgcaccaatt acggcaattc tcataatatc tccattctta tgtctgtttt cacagacagc
    17221 actgtatttc ttcctttact tatgaaaact cagccagtcc ctcaattgca tatgcacgta
    17281 cagggctgga atccagtccc ttgatcggaa gtggagaata gctcatgaag aatacatcct
    17341 tctttaactg ctccagattc tttaatactt ccaaaaatac gatattctca gccataagga
    17401 tatcgtggaa cggctttaca tcctcgttac agttttcaat agaaacgcca tcaccgaagc
    17461 ctacagcctt taccttatga tccttaaacc actgtgcact ttctgcaccc actaagagac
    17521 gctcatcctt gtcagtattg gtatccggag taaatggaac cagcttataa gaagaatcga
    17581 tgatcacgat atctccttct tttaccttac ctgcttcacg atctaaatca gctgctgtaa
    17641 tatggcctct ctttgccaca gtatccttaa aatcaacata tactgctctg cccaggaatg
    17701 tagttaacgg cagatcagca gcagacggcc agttatcatc atgatgataa ggacactcgc
    17761 agtgcgttcc cagatggctc atcaggtcca tatgggtatg gaaatccgga atcgggcctc
    17821 ctgtattaaa acggaaaaga tggcatcttc ttgtctcagt agccgggtcc agaatcttgg
    17881 acagatcaat cactcttaaa tttcccattt cataaacaca cattttattt tcctccttta
    17941 atttagatca ctgttaaaca gtacctctgt ttctacaggc agactggcat ttccgctctt
    18001 ccacccttct gtataccagt ataccggtaa ataaatagga tgtcaagatt agatattatg
    18061 tcaaaatata caacatttat acagatgttt tatgcatatt ttcttattca tcttcctgct
    18121 cctgcggtaa catgggatct ccttgacatc aatctgtctt tgacttaaga ttagcctata
    18181 aaatgatttt gagtcaggtg aatttttatt atgaaagaga aaagcttcct gcagcagcag
    18241 gcatatgata ctataaaaag cagcatttta tcaggcgaaa tggaaccaaa ccgcctttac
    18301 tccgaaacca agctctctgc acagatcggc atatcccgta cgcctgtcag agaagcgctc
    18361 cagtgcctga gccaggatgg atatattaca gttattccca gcaaaggttt tatgatccga
    18421 cagctcaacg ataaggacat gaaggacagt attgagattc gctgtgccat tgaaggcttc
    18481 tgcaccaata tggtcgcttc acaaattgat acagaaaagg gacgacagct gctgcacagt
    18541 ctggaacaga ctctttctga tatggaagag tccctggaaa acggagttca gctggaacac
    18601 tttattgatt ctgatcacca gtttcatctt ctgctggtgg gatatatgga aaacgatgag
    18661 ttcaatcaga ttttccagcg ccttctctat ctgatccggc tcacgtctca ggatgccctg
    18721 gctgtggaag gccgtattca gggaacgatg gaagaacacc gaaattatct gcacgctctt
    18781 cggaacggag acggtcccag agcataccag cttatgatgg agcatctgct gatgccgctt
    18841 aagatgcatg aaaaatgaag ggctctaccg cccttcattt aatatatagt aaatcccgtc
    18901 tttactggaa ttcaacacca gttcctgagg atatcctgcc gcttccagca ttttaaccgc
    18961 ttctgtcaaa tcccctacat catagtaaat gtgggcatcg cttcccaata taacaggaag
    19021 acggtattct ttggcaatag aaagccattt cattacgttt ttatacgcat tttgccgtcc
    19081 agaccgcgga ttgaaagagg aattattgac ttccaaagct accttatgtt ccaatgctgc
    19141 cggaatcaga cggtcatatt ccagtggata tctgtcgtca tcaggatgac cgattatttt
    19201 aacatacgga ttcttcattg ctccaattaa agcgtccgtg ttctcttttg gagttccagg
    19261 cttgatacag ggtatgtgga gactggcaat tacatagtcc atcttcctga gaaccgtctc
    19321 gtccagatcc agatttccat taaaatccat aatattagct tctacaccgg taaaaatccg
    19381 tactcccatt acttcctgac gaaagcattt aaaattgcta aaataaatag aaggagccga
    19441 gccaggcatg gcgggcgcat gatcggacat tccaagagcg aaaagccctt tacctgccgc
    19501 ctcctctaca ttttccttaa aggtgctgaa ggcgtgcccg ctggccacag tatgcgtgtg
    19561 catatcaaaa taaatattca tttcaagttc ctcgtctatg cgcttaatgt gaatgatatg
    19621 tgatctccgg gattatattc cgcctgacag cctgcatata tggtgaggac ttcatcccga
    19681 tgctgaacag aataatgaat ttctcttccg ttatactgct tattgacaat gattccctga
    19741 attccatttc cttccggctg aatagtcagc tgttccgggc gaatcggata aagtccctta
    19801 ttacgaaatt ccatcggaat cataggattt cgcagcaaaa tattttcatc cttaacatgg
    19861 aaggaatctc cacaccatga accttttaac agattacagc gactgacaaa agtagcggca
    19921 aaaactgttt gcggatccct gtaaatctcc cttggcgtgc cgatttgctc caccgctcca
    19981 tccttcatga taatgatcct gtctgccata gccaacgcct cgctctggtc atgggtcaca
    20041 tacagaattg tagctcccgt aagcttatgg atattctgaa tttcccgacg catatcaatt
    20101 ttaagttctg catctaaggc gctcagtggt tcatccatca gaaggattga aggtttcccc
    20161 acaatggccc gtgccagggc aaccctttga cgctgacctc cggaaagttc cccagggaga
    20221 cggttttcgt agggtttcag tccagtacag ctgacagcct gttctattgc ggctttcttt
    20281 tcctctgaac tcatgcctga atgacgcggg ctttttaatg gaaattctat atgctgacgt
    20341 accgtcatat gcggccacag agcaaacgat tgaaatacca ttcccaaatc ccttttttct
    20401 acaggaacca tgtgtccctg tccagaatag atttgatcgt taaggcgtac aataccgctt
    20461 gtaggctcta taaagcctcc aacaatacga agaagggtag tttttccaca gccagatgga
    20521 cccaaaatag caacaaattc cgaatctctg atatccagat taatttcctt gagggcctgg
    20581 gttttgctga atgtttttgt aacctgctgt atatgtaggc tcataaccgt tttcctcctg
    20641 tttaacagat gttaaatgat cgcggttaag cagacagtat cctgctacca ccaaaattgt
    20701 aatcagaacg gacatagctg ccgcaagatt atagtcgccg ccctgctgat agttaaaaat
    20761 agtcagaccg atcgttttag ttcctgcaga agccagcatg gatgacaaag tcagctctgt
    20821 gaaagccgga acaaaaatca taaacgcacc ggacaggatc ggaccggcaa caagcggaat
    20881 aagaatcttt atccatttga tccaaaatcc tcttcctgaa gcagcgcagg cctcctctat
    20941 ggcaggttct acagaaagaa gggcgatggt acttcccttg atctgcagaa ccagatagcg
    21001 ggtaatataa gcaaccacca gaatattaat ggtgccataa attccaggat atacccccgg
    21061 aataggttca acccagtgaa aaatcataga caaagcaaga acaatgccgg gaatagcata
    21121 cgtcagagaa gcacattttt ccagtaaagc tgccgcccgg cttttttttc tgagtttccc
    21181 ataagcgata gccgtacctg ccacaataca tacaagcgta gttaccactg ccaaagtcag
    21241 actggtatga atcgcggaga tcactcctct gttatgaaac acaaaggcaa aattttttaa
    21301 agtcagattt tcaggaacaa aatccagtcc ataagctttc aaaaaagaag atgctgtcat
    21361 ggcagccatg ggaataatgt tcaaaatcaa aagaagcacc agcgttcccc attctaaaca
    21421 taaacgcagt ggatttttta aatgtatccg caccgaatag tcttctttaa tgctgtccat
    21481 ggatgcaccg cgttttacaa aaaatccctc cagcatagta cctgctgcgg cgataaccga
    21541 caggatcatg gacagagccg ctgccagcgg aaacgcattt ggaccaaatc caatggcatt
    21601 ttcgtaaata taggtactta aaaccggaat tccagaagaa attcccagaa aagcaggtac
    21661 agaaaaatta tcaatggcag ataaaaaagc cagtacgctt cctgccgcaa ttgccggagc
    21721 agcggatacc agattaattt tcagtatagc ctgtctctgg gaataaccgc aggctctggc
    21781 tgcccattcc atatctctgg gaatttttct cagcatatga acaacattaa catacacaat
    21841 aggaatatta cagaatccca gtaccagaat gattcctccc atggtgtaaa tatccacacc
    21901 cggaagtccg atagaagtca aaaatccatt gaacgcccct ctggagctga aaaagctgct
    21961 ccaggaaagg gtaatgatgt acgatggaat aataaaagga agcaaaacca ggaattcaat
    22021 aacgccctta cgttttatat tacaataggc ggtgagaaat gccagtccgc tgccacagcc
    22081 agcggccaga gcggtggaac tcacagcaat aataattgta ttaacaatag cttttctggt
    22141 acgctcctga tataaaagtc cggcaaaatt atccagtcca tatccattct ccacggtaac
    22201 tcccatgagc aggagacgga tcaaaggaag aagaaacacc aggactgcca gcagatagac
    22261 ggctatcctg gcaatggacg cccctgtttt acgtatcgtt tccatataga tctcctgtta
    22321 tttactgaaa catctggctg aactgttctt tatctgcttc acggttctga tacagttcct
    22381 tcgtatccca ggtaagaggt gtgatctcat cgatacgctt aaatccttca ggaacttcaa
    22441 ctccgctctt tatgggagta tagccaatct cagcggtcag agcctgaccc tcctcggaga
    22501 gtacaaaatc cacaaatgct ttggccagat cctcactatc cgttcccttt acaattccaa
    22561 tcggttcggt aacggcagga gagccttcag ccgggtatac gaattctacg ggagccccgt
    22621 cattctttga acggatggcc atatagtctg ctaaaagtcc acaggcctgt tctcctgcaa
    22681 ccactgcgtt ctgaacggat ccatttcctt ttcccacaac gactccatta tcctttaatg
    22741 tctgataata atcccatccc agtccttcgg ttctggtaag aacccccagg ttgtaggcag
    22801 cagctccgga atagaggggg cttggcatga tcaggtcatc cttgtaagcc tcatttgtca
    22861 gatctttaaa gcctgcagga atattttcgg ttaactcagt attgtagaca attccagtag
    22921 taattacctt tgttcccaca tacatgtgat ccttgtcaat ataaatttcc ggaataccct
    22981 ccagttcagg agactcgtaa gcgctcagaa gatcctgctc ctttagtgtc tcaaaagtaa
    23041 cattgtccgc caccaaaaga acatctgcca aaacatcccc tgcctccttc tccgccaaca
    23101 ccttgctgac tacttcctca gtaccagaac ggaacacatc cacctgaata tcaggccatt
    23161 ttttattgaa tccgtcaatc atgacctgaa tgtcttcctc cggctgtgaa gtataaagag
    23221 taatagaacc ggataagtct tgtgatactt tttttgaagt ttcttctaaa acagcttccg
    23281 atgagttatc cgcaatctgc tccgaagctg acgcggtagt ttccgaataa ttattctggc
    23341 atcccaccag tgaagatact gccagtcctg ctgttaatac tgctgcaaat ctctttttca
    23401 ttctaactta cctctattaa agatgtgttg tataaactga atattctgtt ttgctataga
    23461 aaagtcccca ctgctgtcca aaccttctat aaccatagga aggttataat gcgccagctt
    23521 aggaagaagc tttttcatat cataatcccc ctcattaaga ggtgtatgaa atctggaacc
    23581 tttgcgatta ctgatatgaa ttttggaaat ctgaggacga taattctgaa gaatacttaa
    23641 aatctcttct tcactgtcac agtgagctac atccagggta taaacaaact ggtcaaacat
    23701 atcgccgcat acctccttca ttgcatccat agtggtaaca aattctctgg gtattttctc
    23761 cataatctcc aacgacacgt ctatcttcca tttctgcgca tattccagaa tttccctcaa
    23821 tgattcctta agataagctc cgtaagatac ccctattcct gaaatagtac aatgtcctgg
    23881 atgaactgtg acttcatcca gtcccagctg ataagcaagt tcaaccgatt ttttcacctc
    23941 agtaacggac tgtttcctga tgccatcatt cacagatgaa atatttaaat cccagctctg
    24001 gctgtgaatg cagttcttta aaggatacag agcagccagc ttttcaaatt catcgatgtc
    24061 aaacttccga tagaaaaatt gttgactcca caattcaatc ccatctaaac cagattgaaa
    24121 aaccatatca aaaatttctt ccatagagcc gttccacagg agagtggaac taagataaat
    24181 gttagccatt ccgattactt cctgtcattt gatgaggcta ttataacagg taaaatgtaa
    24241 agactcctac catgaaaagg taaaaattga gtaaataaat ttcgttttta ttttattgaa
    24301 atttatattg catttttgac aaaactctaa tatattaaac aaaaaggagg ttcatcgact
    24361 atgttggaaa agatttatga agaatataaa gaaagattgt ccaaatcgga tcataagatt
    24421 tttggatatc tgctggagca tgagcaagaa cttccggcta ttactgcaga agacctctca
    24481 cgaaggctga acatcagtac agctactatt tccagattct ggtctaaaat tggatttcga
    24541 aatctaaaag acttaaagca caatttatat gaaaaacagg agtctacccc ttacagccgg
    24601 atgaatgcag ctctaagcca gtggaaagaa tgtggaattt cacctgattt acttctggag
    24661 cgtttgtcct ttcagcttga aaagacattt caagtagtga aaccggaaca ggtggatgaa
    24721 gctgtaaagc ttttaatctc atcacggcag gtctatattt ttgcacccga tgtatcaaaa
    24781 ggtttaggaa acattttaca gtaccgtctg ctgcgtctgg gtattctctt gattcctctt
    24841 ccaggaggct ctcagcttta tgatgctatg gtaaatttga aacagggcga tctggtttta
    24901 ctgtttggat actcacgcct tctgacagaa gtacagattc ttttatccta tagcgaaaaa
    24961 gccggatacc aaaccattct gtttacagat ttactggccg aagaacattt atctaacgcc
    25021 aatttaacat tgtacagctt ccgtggggaa ccaaatgact atcactcagt agcaaccccc
    25081 ttactattga tcgatttgtt gattatgaaa gccagccaat cccttggggg cggattggaa
    25141 aaagcgaaga aacttcagaa tttgagagaa caatacgcag gattaattcg acgataaaat
    25201 acgaagaaca taacatcaat aattctctta aaacacaaaa atatctccag ccgcataact
    25261 acgactggag atgaacaaaa ttttactttt tatccgccta cttggcaaca ggcgatttaa
    25321 aacaataaca gtttcatcta atctcataac tcaccactta taatacttaa tcaacgcctg
    25381 agttccaaga tctcccatac cctctgattc cagctttttc gccattccca gcacatcctc
    25441 caaaactccc agacgggtat ctgcagctaa tgcctcttca tctgcaattc ccatatcttt
    25501 gataaaatgt ttcaggaaaa agccgggggc atagtcatct tttgctgcct tagaagccac
    25561 attattcatc tgggcactgc ctgctgcacc ggtagaaata gatttcagca tggtatctac
    25621 atccagcccc gtattctttg cataaaccat agcctcacag gcgcctgcca gagcgcctgc
    25681 aatggcaatc tggttgcaca tcttggtatg ctgtccgttt cctgcctttc cctcgtaatt
    25741 aatattcttt cccatagcct caaaaacagg cacacaggcg tcaaaatcct ctttcttacc
    25801 accagccagg atcgtcaatg tacctgcttt ggcaccggta tctcctcctg ttacaggggc
    25861 atctactgca tgaagccctg ctttttctgc ctcttcccag atacgcacag ccagtctggg
    25921 gctcgtggtt gtcatatcaa tcacataggt tcccggatca gcatttgcaa tgattccatt
    25981 gtcaccaaaa taaacctctt ccacatcctt tggatagccc acaatggtaa tcactacgtc
    26041 acgtcccttt gcacactcag ctgctgtatc acaccatact gcaccctctg caatcacatc
    26101 ctcagcctta gcctttgtac gggtataaat ggcaacctca tatcctgcct ttctcagatt
    26161 ccgcaccatg gattttccca taattcccac accgataaaa ccaatttttt tcatttttat
    26221 ttatctcctt tcgcttctat atcttttaaa ataagctgaa tacaggtatt gaaatcattg
    26281 ttttcatacc ctctgtcaat cccgcacgct gccaggcgtt cttttgaaaa gtccttctca
    26341 tctgccagaa atctccggca catctccgca tatttaggcg tattctgcgt tttttcccga
    26401 tccagtgccc tctgaaggcg cgtcccatca tccacataga tataaagagg aatcataact
    26461 tcatttccgt aataatttac catcttttca taagaatcca gtgttcctat cataagataa
    26521 tttccctgtt tcagatcgat ctggccatcg tcagcggtaa agtaactcca tggtccaaac
    26581 actgtatcat aggttctgca ctcaatcacc ttgccctgac tctccagctt ctttaaacgt
    26641 gcttcatctg taaaataata ctccacaccc tcctgttccc cgtccctgat gggccgggtg
    26701 gtgtacatac gcactgtgcc gtattcagga aaccggcgca ccagctcctt gtaaatcgtg
    26761 tctttaccgg cagcactttt tcccatgaca taaaaaatat atcccatttt cctgttcttc
    26821 ctgttaaata ttctgtctaa atggcctgtt ctgatacaag tattgtctcc agcgtctgat
    26881 ccgacagccc ctgtaccgga agccccttcc tgatataatc cagaatcacc tccagaagct
    26941 gctccgttat acattccccc ggagcaatca gagggatacc cggcggatag atatagacaa
    27001 attccccgga tattttccct gcgctttccc tgagaggtat tctcgcagtt tttccatcaa
    27061 tggcctcccg gatggagcac cgggtctttg cctctatggc atagacgcag tcagagccgg
    27121 ccccttcccg gccctgtaat tccattttcc cggaagggaa gccttcctta tcaatagcaa
    27181 gaagggcagc tctcagccgt tcaagtcctg ctttactgtc tccaacagcc gttatagctg
    27241 tcacataatc tgccccgcac atttccattt ccagatggta ctgtttccgc agaagctccg
    27301 acagcccctc cccgtcaaag gacgcatacc gggtagatat cacaacttta gaagggtcca
    27361 gatcaaaaat tccataccgg ccttttaatt cccttcccac cagacgcagg ttcttcatag
    27421 cagacaggct ttctctcatc tccaaaaggc tgtctgcaaa cttcctcata ccatccttcc
    27481 tgcgcagctg ctccatctga aatataccat tttcaataga tgccattaac acataagacg
    27541 gactgctgct ctgaactata tgcaggtacc gttccacagc ttccatatcc atataggcgc
    27601 ttccatccgg tccattgcat ttcatatgaa gcacagccgt ctgggtcagg ctgggaaggg
    27661 tcttatgtac gctctgtatc actacatctg ccccaagctc cagagcagac tgcggaaata
    27721 tttcactgta tctgaaatga gccccgtgag cttcatccac aatcagagga agtccggccc
    27781 gatggactat ttttgcaatt tcacccacat ctgacacaat tccatcataa gtaggcgaca
    27841 cgatgagaac cgcccgtata tcaggctgct ccttcaggga cttttccaca tcctccggca
    27901 aaattcctcc ctgtatcccc cattcctttg tggactgtgg ataaacgtat gaaacctgta
    27961 aatggtgaag gtaaatccca ttatatactg ccttatggca attacgcccg atcagaatcc
    28021 gttctcccca atgcacacag ccgcatacag ccgccaaaag ccccgatgta cttccattta
    28081 ccagataccg ggtacggtct gctccataca gcccagcagc ccactccata gattccttta
    28141 aaattccctc cggatggtga agattgtcaa agccgtcaat ttctgtaata tctacctcaa
    28201 aggggtttgg aaattccatt ccctgataaa aggcccttcc atccgcttcc tctccccatt
    28261 tcagtctctt atggcccggc atgtgaaagg gatatatccc tgtttttcca tagtgcttta
    28321 aacggttaat cagaaggtct tcttctctca tatggtcctt cctcccgtac tgagccgatc
    28381 ctttctccgt tccaccacct gaatcatcac actgcggagc tgacgccggt tttttaccac
    28441 cagagtcttt tcctgatagc gttccgcgat actgcggtac tggcgcttct tctttctgct
    28501 gcgccccttg tgaagaatcc acca
//
LOCUS       DBMDUH010000013        28195 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_1209_length_28195_cov_6.792482, whole genome shotgun sequence.
ACCESSION   DBMDUH010000013 DBMDUH010000000
VERSION     DBMDUH010000013.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 28195)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 28195)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28195
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1209_length_28195_cov_6.792482"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="ACZPCG_02425"
     CDS             <1..623
                     /locus_tag="ACZPCG_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIR1283961.1"
                     /translation="QTGCGTVDIMMHTLERYFSLGHDTSLTDRMAEGLLKAVIEAGRI
                     VHENPEDYEARAALMWAGSLSHNDLTGAGRQVFMPVHQLEHELSGMDDRIAHGAGLSA
                     LWASWARYVYQNDMNRFAQYAVNVWNQEMDFEHPERTAQAGIRETEDYFKSLDMPVSL
                     KELGVDPASFKVMAEKCSGGGARRLTGIRELDEEDMIRIYEMAMGE"
     gene            complement(724..1527)
                     /locus_tag="ACZPCG_02430"
     CDS             complement(724..1527)
                     /locus_tag="ACZPCG_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007708438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR1283962.1"
                     /translation="MEELYVFGTGNAQAVHCYNTCFALKDEDEFFMVDAGGGNGILKI
                     LEEMNVDLSHIHHIFVTHEHTDHILGIVWMIRMIAAAMKKDAYRGQLQIYCHPDLTET
                     ISTLCRLTLQGKFYNMIGERIFLVPVEDEETVHILDYDVTFFDILSTKAKQFGFTTVL
                     KNRKKLTFAGDEPLNRECVSIVKGSSWLLHEAFCLYKDRDRFKPYEKHHSTVKEACEL
                     AEELKIPHLLLWHTEDKQISERKALYTAEGHTYYRGELYVPDDREIIAL"
     gene            1798..3735
                     /locus_tag="ACZPCG_02435"
     CDS             1798..3735
                     /locus_tag="ACZPCG_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HIR1283963.1"
                     /translation="MRDVAYLQLLAREFPSVKGATGEIVNLMAICSLPKGTEYFFSDL
                     HGEYEAFIHLLRSSSGIIREKIKETFGYVIPEEEQLQLANLIYYPERHLPRMMKQGRY
                     TEDWQRITIYRLVQICREVASKYTRSKVRKKMPKEYAYTIDELLHVDGNDENKKLYYN
                     EIIHSIIDTSIADKFIAALCRLIQNLTIDNLHIIGDIFDRGPRADIIMQELMSFHDVD
                     IQWGNHDISWMGAAAGNLACICNVLRIAISYNSFDVLEDGYGINLRPLSMFAARVYQD
                     DPCERFMPRILDENLYDAVDSGLAARMHKAIAVIQFKVEGAMIMRHPEYGMEDRLLLP
                     AVDYEKGTVTIGGRAYPMLDTNFPTIDPRNPLELTRGEKELLRVLSASFRHSEVLHRH
                     VRFLYSHGSIYTCCNSNLLYHGCIPMRKDGTFEGLTFGGKEYRGKELMDYLGRQIHNA
                     YFMPDGQQEKQEALDLMWYLWCGAKSPVFGKDKMTTFEHYFVEDKAAHKENMNPYYKL
                     SESEEVCCRILEEFGLPVEGSHIINGHVPVKLTDGEKPVKAGGKLFIIDGGLSKAYQS
                     TTGIAGYTLIYNSHQLSLAEHMPFDPKKDSTPKVSMVERMPRRIMVADTDKGRELKAQ
                     IADLKELVAAYREGLIKERAE"
     gene            3736..4248
                     /locus_tag="ACZPCG_02440"
     CDS             3736..4248
                     /locus_tag="ACZPCG_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HIR1283964.1"
                     /translation="MTKNEFLQGLKEELEGRVTHSAIQENIRYYDDYISQEVLKGSTE
                     EEVTESLGGPRIIARTIVDAAYDTEDRPDGYDEYGSGPAEESEAGTGSRSPFGSQENP
                     YTGHSHIHFLDFSKWYVRLGAGLVVFCVIFLIMSVFFGIVGLAGFILAHIWPVLVILM
                     IIWMFRGPRR"
     gene            4249..6564
                     /locus_tag="ACZPCG_02445"
     CDS             4249..6564
                     /locus_tag="ACZPCG_02445"
                     /inference="COORDINATES: protein motif:HMM:NF024242.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1283965.1"
                     /translation="MEKTKKSRYQRKLFAYFMAVAVIPLCILGMFSYHTAKEALRENI
                     HHGNEAALAQIENKTEKALEMIRIDFLGTAVSPVTQRMINSAYDEIPYPELRDFKDGM
                     GGQESYSDYVKSYSFINLKHGWILGNKGYIPLEDVHNLQWIEILEKNPEKIFWLNETG
                     WNQEGKTDDVARETLTEQQKITPEYISHEYLTLVIKVPTYSEKSDAMFVVSLDQAYME
                     ELFRGSIGNGALLILDPEGNPVYSEDEKLTEAYLDESGEKKADYDIVSRTADMSGWTY
                     VAGYDSRYADGQMNQIIVTMAALILFVFVLLIIMSILGAFRVYQPVKELVSQVKKIVP
                     EQPQAEGEFDLIRQGLHALTNSNEELQTMIRRQKSQLTELFAFRLIRGRMGETEVRES
                     MGRLGLQFQPCLYVVSVLFCKNGRDENTEQIEQDVLNLELMKQLPEEILTMLAIPPFI
                     HTHAIVMVLDGPGKEKLEQKLLALRNCLLVFAGTESGGWLNMGVSRSFEDLSHFRGAY
                     FESLEALKINEHCDRDEDTEGISIEESSVTYYEDLIRQEEEAKRYDLALDTAIKAAVD
                     SCDQKAAFEVVDTFVREVEKRGVALYEQHYYLNRFLLAILTVPADAGIPVHDLFGEED
                     DPFRQASQLFDYAAIRSFYKKKVVQPVIGRMNQFRKSGSEMILEKVMALIDQKNGDVT
                     LAECAQELGYHPSYIWRVLKNTRDITFTDYIAEQKLRIATRLLEETELSVSEIAERLS
                     YSNAQNFIRLFKKHMGITPGQYRKHRREKAQ"
     gene            6923..7828
                     /locus_tag="ACZPCG_02450"
     CDS             6923..7828
                     /locus_tag="ACZPCG_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012198369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1283966.1"
                     /translation="MADKLAYLKRHKWMYFLMLPGIIYFIIFKYVPMGGLIIAFQNYS
                     PYLGIAGSEWVGFEHFKNFFMNPDFKMLLVNTFSISLMNLLFFFPAPIILALLLNEVK
                     NKLLKRSIQTMIYIPHFISMVIVASITFTLLNAENGVITQLSTMITGENPEFLSDPKY
                     FRWIIVLQNIWKETGWGMIIFLAALSGVDTQLYEAAEMDGAGKLRQMWHITLPAIKST
                     IIIMLILKVGTLLNTGFEQIFLMKNSLNASVAEVFDTYVYTLGITQGAFSYSTAVGMF
                     KSVVATIMVLATNWICKKCGETGLY"
     gene            7846..8757
                     /locus_tag="ACZPCG_02455"
     CDS             7846..8757
                     /locus_tag="ACZPCG_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1283967.1"
                     /translation="MTAKSKTKRTKMKRSPQERIGLFIVHVICITAGVLTVVPFLYVL
                     SGSFATEKELLERSFFIIPHTISTNAYKYIVADGSIFRGLLNSTLVTVVGTVVNMLFT
                     TTLAYPLSKSWLKGRNVILNMVVFTMLFSGGMIPNYLVVKGLDLLDSYWALILPGAIS
                     AFNLIIVKNFFQELPGELEEAAKIDGCSDLGIFTKIVLPLSKPALASVGLFYAVSHWN
                     DFFNSMIYLNSTEKFPVQIILRQIVLLAQGASADGSAIDFGAGGTPPEQAVKMAATVV
                     ACLPILCVYPFVQRYFEQGVMVGAVKG"
     gene            8782..10323
                     /locus_tag="ACZPCG_02460"
     CDS             8782..10323
                     /locus_tag="ACZPCG_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 periplasmic-binding domain-containing
                     protein"
                     /protein_id="HIR1283968.1"
                     /translation="MKKSTWKKGLSVTLAAAMTAGLAAGCGSKKEASSGTAEDGKYNL
                     KIMSYDFFGNPMKGDVGAGIMKQVEDYTGTHLDIMWTPKDNYDDKLNLVLAGGGDDMP
                     QIIATDTKSPAIINAARAGALWDVEELMKEFPHLKNAKPEVNDNIRIDGKLYGVYRGR
                     ALGRNGLTFRKDWADNLGLEAPETIEDVYNMLHAFTYDDPDGNGQDDTYGLALSKSTA
                     PIDIIQTWFGAPNGWGEKDGKLVPEHQTEEYLEALNWMRKLCEEGLIKKDFPTRDIAT
                     KSDDLKTQKAGMMVDALDDGRRVQDYFENQGIEGPEINFVGAIKKDEASEPRTGATLG
                     CQGFFVITKAAKTEEDVRKCLDFLDKMNDEEMLTLANYGLEGNHHTIEADGRLTRSHD
                     ATLNQEYQALNQLVSYTEYPPNMDPYVELNETEYYYMQQDTIAANEQYAVSDPAAGIL
                     GDSEEYIKNGVALDKIIEDARVQYIVGQIDETQLKAQWDLWSQSGGDKVIEEVNAKYA
                     ELKNK"
     gene            10441..13626
                     /locus_tag="ACZPCG_02465"
     CDS             10441..13626
                     /locus_tag="ACZPCG_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1283969.1"
                     /translation="MVSEKAFANPPVSSRIKPFWFWNGTMTREEISHQIKEMADKGLG
                     GMFICARQGMDVPYLSGKWFELVDFACREAKRYGLEAWLYDEYPYPSGMSGGEVLLEH
                     PEAEHKILKHKKFRTEGKIRVTEELGWSRILYAKAFPVDENGKTIWENPLDLESSVGI
                     LQTEKIYQQTGLTRYNNKRFFSYGPKKILDAVLPEGQWEIQICTEEAMGDFKYYGGFF
                     DPCNREAVKTFLDTTHERYAKTNGEEFGVSVFGMFSDEVGLLSPIPWSSRLPEAFKVR
                     NGYSILEVLPGIYEPDYPGAAKLRYDLYETAHELFRESYHHQVASWCEEHHLLYATEV
                     PSMRHSTQKFSHIPGGDTAHEKLGKSLEWIYDEYIRNYRSNAKAVSSLARQLDRKYAM
                     IESFHSVGWTMNMQDAKWMIDRLGSSGINLYNFHAFYYTIQDITKHDAPPSQFLQNPY
                     WKDYRKLADYVGRMGEIITNTEADIHIAVLDPIAAMWTLLGNPFQGFVYKGESEEEKA
                     ACDRIRDGWVAVCKELLFAQLDYDHLDGELLEEAEICDGSIRMGRAAYSVVVLPECHC
                     MEKKTREMLERFVQAGGHVIAVGELPSVSIDKEETDQETEAAWKALTAHANVVCLNGS
                     REALPDCCRGYIREAGRVEVLEGDRKSVIGVCRQDEAGSVYLFAANQGAEPVKVRFSS
                     ENGTNSAVWMDLEKGRQIPAENPENKGIAVTLEGFESRWLKLSDFEEEAVGREPEGTL
                     GNSCLSLVQASMMDEGGRSRCAKHISMAGKWKCFPQSLNVCRFGETEVSVDGTNWEKV
                     EVKTFIEQCAQTKRLGGEQIAMEGEFGTPRKLRPAYPITCFYKVGFKIRKGELPSELF
                     LLMDRETISGSNAIEINGRVVEASLWKPVRVNDFNNQAADIRSLVREGENEIRIRVCI
                     RKDEDGLRDPFYLWGSFGVGFEDGCHVLTAQPEQVCPDAPWIQGFPYYSGTMEFETEI
                     EFDSSEACILALDWAYPCHENIEVLVNGRSTGICAYSPYLWECGQGTAKQGMNCVTVR
                     ISNTLANMLDGTYFDYKEHRLADIRQEAEER"
     gene            13623..15755
                     /locus_tag="ACZPCG_02470"
     CDS             13623..15755
                     /locus_tag="ACZPCG_02470"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019113.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88/105 protein"
                     /protein_id="HIR1283970.1"
                     /translation="MTTYFDREQSIEYRLGSETGPVLQTIANRFCADNPAVPVQYSLR
                     STQGFSITKDYLDEILLHEKFPQMEREQYVYARAGLTPQAPGPFHFRFGAYGPVTIYV
                     NGKKSFQTNHTQERFSETLSDICLDLKEGVNDLLFECVSTPLGCGFRIGSSSYKGRRI
                     QFFAVDEAHKGMNGFVYTEPQKTAMDPADADSVCWLPRAVWTEEEKQESVCSRLFDGK
                     TMLSVTRVFVPQEGMIRILGACGRTGQEPVEVFADGKRAGTSKDDSLTLELSLASGYH
                     TLAFLGGDPDVTVEDRQGTVLELEAPVLCMGQPQTWIYAGPFDRVRPSEEEISRMLSF
                     KKPFETDLGKEFWRVDGPDLYLRPVNEGTLYGEWNYPLGVTLYGMIQTARYEHDNALE
                     CYVRGHMEKCTGFYEYCMWDKEHYGAAPFHNQLTTIDSLDDCGSFASALLEVMKDYEI
                     PEGDVISAMVADYMKDRQQRMEDGTFYRNHSYLPVMNETIWADDLYMSVPFLCRYYKR
                     SGDECWLKEAAGQLKRIFGYLYMPEEGVLSHIYDTHYGVQTKVPWGRGNGWALFSAAE
                     LLSVMPKEHENREEILDIYRTLCRGYLKVQDPDGMWHQVLTMKESYEETSCTAMFIYG
                     FAKGVKNGWHTDGEAYRKAAIKGWRALCRTSIDWKGNIYGVCRGSGYSFSREYYAYEL
                     GWNFNDTHGTGIVLLAGIETERMLCPEE"
     gene            15772..17097
                     /locus_tag="ACZPCG_02475"
     CDS             15772..17097
                     /locus_tag="ACZPCG_02475"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006775613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HIR1283971.1"
                     /translation="MEQIFDPREFGAAGDGKTLDTQAIQAAIDAAAKEGRDGKRSVVC
                     LKAGTYLTSSLFLKSHMEFRMEDAAVLLGTTEEEQYPIMRNRVAGIEMDWPVGILNVM
                     DQEDVQITGEGCIDGQGPYWWNKYWGEDQKGGMRAEYDPMGLRWCVDYDCRRVRNLVV
                     MDSRRIEIAGIGSRRSGFWNMHICYSEDVHVDGVWIRDNEGPSTDGIDIDSCRHVVVE
                     NCRVACNDDSICVKSGRDADGLRVNRICEDVLIQNCQVLTGCGVTLGSETSGGIRNVT
                     IRNMKYHGTDCGFRIKSAATRGGVMEDILVEDLEMVNVKYPINMCLNWHPAYSYCEIP
                     KGYEGEIPEHWKVLAQSVSREMGVPQVKNLQIRNVRSWNEEGYEGCSRAFEVDAFPER
                     PMENVVLEHVDIKAKEFGRIAGIKNWQWKDVNISVEGRNQDENNVYDVR"
     gene            17287..17934
                     /locus_tag="ACZPCG_02480"
     CDS             17287..17934
                     /locus_tag="ACZPCG_02480"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007708457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HIR1283972.1"
                     /translation="MMDPQESMAAARTEVQTRSSYMVTIEAPSVDVYTYKNKDSKTVG
                     QVKRGQTYEVLKKEQDGWVQIRMGNREGYISAAGNASLVEKNRQTVDSGARQRREVVE
                     FALQFLGGEYKYGGTDPNKGVDCSGFTRYVMKYGASVEIPHSSTGQAACGTEVSEEEM
                     QPGDLIFYSSPFGRINHVAMYAGNGQVVHASTEETGIKLSPYNYREPVKIVSVLE"
     gene            18057..18779
                     /locus_tag="ACZPCG_02485"
     CDS             18057..18779
                     /locus_tag="ACZPCG_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HIR1283973.1"
                     /translation="MHNFFRKLVGTGVVCGMLVFAAPLTSMAAIGPGFAAGTYVATVT
                     AESVNINKNRDSEEVLFTAKAGSTYEVLEDCGDGWMKVRVHDTEGYLPVSENAVVEEA
                     EEGEIAMIQKEARESSASYKRQQLADYALQFVGGPYQYGGSDPHTGVDCSGFTRYVYQ
                     HGAGITLNRSSRGQALQGKEISADQMRPGDLLFYGSKSNIDHVAMYIGEGKIVHASTE
                     RTGITISNWNYRNPVKIRSIME"
     gene            19101..19286
                     /locus_tag="ACZPCG_02490"
     CDS             19101..19286
                     /locus_tag="ACZPCG_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1283974.1"
                     /translation="MSSRNDNMNSYSNSQNNAQNNSRNNSSNNASNGRSKNNMKNSAE
                     NKYENCGRNNARNDSND"
     gene            19474..19791
                     /locus_tag="ACZPCG_02495"
     CDS             19474..19791
                     /locus_tag="ACZPCG_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR1283975.1"
                     /translation="MISYKQLDQWLEQGNVRLLADVREPWMYGENRILGSVNIPYEEV
                     RERLEEFPRQGMIVCYCDRGAKSMVVCRDLWRLGYEVSDLAGGMLDYRGKYVDRRPLW
                     DLK"
     gene            19981..20535
                     /gene="yfcE"
                     /locus_tag="ACZPCG_02500"
     CDS             19981..20535
                     /gene="yfcE"
                     /locus_tag="ACZPCG_02500"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HIR1283976.1"
                     /translation="MKLLFASDIHGSAFYCRRLLDIYKESGASRLVLLGDILYHGPRN
                     DLPKEYAPKEVIAMLNPLKNQIYAVRGNCDTEVDQMVLEFPILADYALFSVEGKTLYA
                     THGHIFNQDNLPPMQEGDILIHGHTHLLKAEETEGDCGRIRVLNPGSVSIPKGGNPHT
                     YGILEDGVFTIRTLDGEAVKEMKL"
     gene            20552..21727
                     /locus_tag="ACZPCG_02505"
     CDS             20552..21727
                     /locus_tag="ACZPCG_02505"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007708504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing class I SAM-dependent
                     RNA methyltransferase"
                     /protein_id="HIR1283977.1"
                     /translation="MKQTYELIAPCHFGLEAVLKKEILDLGYEIASVEDGRVTFIGDD
                     EAICRANVFLRTAERILLKAGSFKAETFEELFQGTRAIAWEELIPEDGKFWVAKASSI
                     KSKLFSPSDIQSIMKKAMVERMKSHYKVTWFPENGASFPLRVFLYKDIVTIGIDTSGD
                     SLHKRGYRTLTSKAPITETLASALILLTPWNKDRILVDPFCGSGTFPIEAAMIAANIA
                     PGMNRSFLAEDWKNIIGRKCWYEAMDEANDLVDDSVQVDIQGYDIDGDIVKAARLNAQ
                     SAGVDHMIHFQQRPVSALSHPKKYGFIISNPPYGERIEEKKNLPVLYREIGERFAALD
                     AWSMYLITSYEDTEKCIGRKADKNRKIYNGMLKTYFYQFMGPKPPRRSQEKKIDKQE"
     gene            21768..23174
                     /locus_tag="ACZPCG_02510"
     CDS             21768..23174
                     /locus_tag="ACZPCG_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin like domain-containing protein"
                     /protein_id="HIR1283978.1"
                     /translation="MTAGCAVLADKRSPDSSFGYEDVRGQETEQTEQSLSALPEVGEK
                     DASCQIWNRGLPEDTILPRSYDYREEGRAPQIGNQGSLGTCWAFASLTALESSLLPEK
                     NESFSVDHMSMHNNYTLGQDEGGEYTMSMAYLLGWQGPVWESEDPYGDGVSPCGLKPR
                     LHVQEIQILPSKDYEAIKRAVYLEGGVQSSLYTSMQDYDSESVYYNRDTNSYCYVGTN
                     EPNHDSVIIGWDDDYPKENFNMDLEGDGAFICTNSWGENFGDEGYFYVSYYDTNIGVH
                     NIVYTGAESAGLYEKIYQSDLCGWVGQIGYGQDTVWATNTFRAGEKEELEAAGFYATG
                     PDTEYEMYLARYLPEVQEGISGAEKESQSARMDRALNDRRPIARGKLMYSGYYTVPFD
                     EKILLDAGEKFAIIVKIKTPDTVHPAAIEYDAGDGIARVDLTDGEGYLSHDGSLWERV
                     EETQKCNLCLKAYTNTKG"
     gene            23181..23801
                     /gene="rdgB"
                     /locus_tag="ACZPCG_02515"
     CDS             23181..23801
                     /gene="rdgB"
                     /locus_tag="ACZPCG_02515"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035870 - dITP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0036222 - XTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HIR1283979.1"
                     /translation="MEQKIIFATGNQGKMREIRAILSDLGLPVLSMKEAGVSLDITED
                     GTSFAENARIKARAVWEHTGGIVLADDSGLAVDYIGGEPGVYSARYMGEDTSYEIKNR
                     NIIDRLLGAEGKERSARFVCNIAAVLPDGRVIQTEAYMEGVIAKEPAGTGGFGYDPIL
                     YLPEFGKTSAELTMEEKNRISHRGKALEAMKSALKQEIAVTGGRMA"
     gene            23823..24323
                     /locus_tag="ACZPCG_02520"
     CDS             23823..24323
                     /locus_tag="ACZPCG_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR1283980.1"
                     /translation="MKILIVSDTHRKDGNLQWVMEKEKPFDMLIHLGDAEGSEQYIAH
                     WAEPGCELEMVLGNNDFFSHLERERDLMIGKYKTLLTHGHYYHVSLSTEYLKEEARSR
                     DFDIAMFGHTHRPFFEIDRKKGQKDLIVLNPGSLSYPRQEGRRPSYMIMEIGEDGEAA
                     FNHYYL"
     regulatory      24513..24609
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            24711..26480
                     /gene="ade"
                     /locus_tag="ACZPCG_02525"
     CDS             24711..26480
                     /gene="ade"
                     /locus_tag="ACZPCG_02525"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HIR1283981.1"
                     /translation="MEGKSSRQDSSSLRERILAASGEKRAGLVLKGARVVNVFTGELE
                     EADVAIEGGYIAGVGSYEGETEVDLSGKIICPGFIDGHIHLESSMVRPAEFERAVLPH
                     GTTAVITDPHEIANVAGTEGIDYMLYETENLMLDVFFMLPSCVPAADLEESGAVLRAE
                     DLRAYYENPRVLGLAEMMNAPGTVKAEEGVLAKLKDCSDRGKRIDGHAPGLSGYQLNA
                     YCAAGVASDHECSEAEEALEKLRRGQWIMIREGTAARNLEGLISLCKQPYVNRCMFVT
                     DDKHPGDLQRKGHMDGIIRKAVRLGADPVTAIQMATLHPARYFGLRENGAVAPGYRAD
                     LVVLEDLEQIKAVQVYKNGKLAAQMGELISQEKRREKSESPSFDRVHHSFHMKEVCEQ
                     DFRLEPEGAYCRVIELTPGELLTKERNAAWVQLPGFAPGVNPQEDIIKLAVLERHKGT
                     GHKGIGFLGGYGLKAGAVATSVAHDSHNLIVAGVSDRDMALAAETVRKAQGGIAVVKD
                     GAVLGLLPLPIGGLMNPMAAEAVDEKLEELKRLAAGLGVREGIDPFMTLAFVSLPVIP
                     KLRLNTCGLIDTQLQEIRAVSFS"
     gene            26529..27902
                     /locus_tag="ACZPCG_02530"
     CDS             26529..27902
                     /locus_tag="ACZPCG_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015851 - nucleobase transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HIR1283982.1"
                     /translation="MDLEKIFHLKENKTDVKTEVIAGITTFMTMAYILAVNPSVLSAC
                     GMDSGAVFTATALAALIATLLMAAFSNYPFVLAPGMGLNAYFSYTVVLQMGYSWQTAL
                     AAVFIEGIVFILLSLTNVREAIFNAIPMNLKHAVSVGIGLFIAFIGLQNAHIVVDSAT
                     LVGVYSFKSSVENGLFNSEGITVVLALVGILVTGILVVKGVKGNILWGILITWGLGIL
                     CQLLGIYQVNPEIGMYSLLPDFSNGISVPSLAPTLMQMDFSGILSLDFFVVMFAFLFV
                     DLFDTLGTLIGVASKADMLDKDGKLPRIRGALLSDAIGTSVGAMLGTSTTTIFVESAS
                     GVAEGGRTGLTAVVAAILFGLSLFLSPIFLAIPSFATAPALVVVGFLMLTSITKINFD
                     DFTEAIPAYVAIIAMPFMYSISEGIAMGVISYVVINVVTGKMKEKKISPLMYVLAVLF
                     IGKYIML"
BASE COUNT         7729 a         5705 c         7860 g         6901 t
ORIGIN      
        1 tccagacagg ctgcggcaca gtggatatta tgatgcatac tctggagcgg tatttcagtc
       61 tgggacatga tacaagcctt acagaccgta tggcggaggg attgttaaag gctgtgatcg
      121 aagcaggaag aattgtccat gaaaatccgg aggattatga ggcaagagcg gcactgatgt
      181 gggctggaag cctttcccac aatgacctga ccggagcagg aagacaggta tttatgccgg
      241 ttcatcagtt agagcatgag ctgtcgggaa tggacgaccg gatcgcccat ggtgcaggcc
      301 tgtcggcgct ctgggcttcc tgggccagat atgtatatca gaatgatatg aaccgttttg
      361 cgcagtatgc agtgaatgta tggaatcagg agatggattt tgaacatccg gagcgtacgg
      421 cgcaggccgg tatccgggaa acggaagatt actttaaatc cctggatatg ccggtgagct
      481 taaaggagct gggcgtggat cctgcatcct ttaaggtgat ggcagaaaaa tgctccggag
      541 gtggagcacg caggctgaca ggcatccggg aactggatga ggaagacatg atccgcatct
      601 atgagatggc tatgggtgag tgagaccgta cagtgatata ggaaatgctc cgggcagttt
      661 atgagactgc cggagcattt tttcgctgct gtaacttgta tcaaggagat cctgcagccg
      721 gatttacagt gcgatgatct cacggtcatc cggcacatac agttctcccc ggtaataggt
      781 atgtccttct gccgtataga gcgccttgcg ttcagagatc tgtttatctt cggtgtgcca
      841 cagaagcaga tgggggattt taagctcttc ggccagttca caggcctcct ttacggtgct
      901 gtgatgcttt tcatatggtt taaagcggtc tctgtctttg tagaggcaga aggcttcgtg
      961 gagcagccag gagcttccct ttacaatgga tacgcattcg cggtttaatg gttcatctcc
     1021 ggcgaatgta agctttttcc tgtttttcaa aaccgtggtg aagccgaatt gctttgcctt
     1081 ggtagagaga atatcaaaaa atgtcacatc ataatccaga atgtgtacgg tttcttcatc
     1141 ctctacagga acaaggaaaa tccgttctcc gatcatgttg taaaatttcc cctgcaatgt
     1201 caggcggcag agggtggaaa tagtttcggt aagatccgga tggcagtaaa tctgaagctg
     1261 tccccgatag gcatcttttt tcatggcagc agcgatcatg cggatcatcc atacgatccc
     1321 caaaatgtga tccgtgtgct catgggtcac gaaaatatgg tggatatgag aaagatccac
     1381 attcatttct tccagaatct taaggattcc gtttccgcct ccggcgtcga ccataaaaaa
     1441 ttcatcctca tcttttaagg caaagcaggt attgtagcag tgcactgcct gggcgtttcc
     1501 agttccgaat acatataatt cttccatagg ttttcctttc taaacgagtt tatcataaat
     1561 ttttaaatca tgttttatgg gaaaaacttt aaaatatgct acaaatatgg tactataatc
     1621 ctagcgaaat tgcaataagt gtacagtttt gagtttccgc atttcgcacc gcaccctgga
     1681 gtctggtgtg cagggttccg gttttctaac gacctacaaa gtcaccctgc acaccagact
     1741 ccagggcaca ctgcaaaatg cggaaactca aacaatcgac gtacggggga atgtattatg
     1801 agagacgtgg catatctgca gcttctggcg cgggaatttc cttctgtgaa gggagcgaca
     1861 ggggaaatcg taaatctgat ggcaatttgc agtctgccta agggaacgga gtattttttc
     1921 agcgatcttc atggagaata tgaagccttt attcatctgc ttcggtcttc ctccggcatt
     1981 atcagggaga agatcaagga aacttttgga tatgtgatcc cggaggagga gcagctgcag
     2041 ctggcaaacc tgatctatta tccggagcgc catctgccca ggatgatgaa gcagggcaga
     2101 tatacggagg actggcagcg tatcaccatt taccggctgg tacagatctg ccgggaggtg
     2161 gcatccaagt atacccgttc caaggtccgc aaaaagatgc cgaaggaata tgcatatacc
     2221 attgatgaac tgcttcatgt ggatggaaat gatgaaaata aaaagcttta ttacaatgag
     2281 attatccatt cgatcataga caccagtatt gcggataagt ttatagcagc gctctgccgg
     2341 ctgatccaga atcttaccat tgataatctc catattattg gggatatttt tgacagaggc
     2401 cccagggcgg atatcattat gcaggagctg atgagcttcc atgatgtgga tatccagtgg
     2461 ggaaatcacg atatttcctg gatgggggct gctgcgggca atctggcctg tatctgcaat
     2521 gtactgcgga tcgctataag ctacaacagc tttgatgtgc tggaggatgg ctacggtatc
     2581 aatctgcgcc cgctttccat gtttgcggcg cgggtatatc aggatgatcc gtgcgagcgt
     2641 tttatgccca ggatcctgga tgaaaatctt tacgacgcag tggattccgg actggctgcc
     2701 aggatgcaca aggcaatcgc tgtcattcag ttcaaggtgg aaggagctat gatcatgcgc
     2761 catccggagt atgggatgga ggaccggctc ctgcttccgg ctgtggatta tgaaaaggga
     2821 accgtaacga tcggaggaag ggcctatccc atgctggata ccaatttccc gacgatcgat
     2881 ccaagaaatc cgctggaact tacaaggggg gagaaggagc ttctgcgggt gctgtccgcc
     2941 tccttccgcc acagcgaggt tctccatcgt catgtgcgct tcctctattc ccacgggagc
     3001 atttacacct gctgcaattc caaccttctt taccatggct gtattcccat gagaaaggat
     3061 ggtacctttg aagggcttac ctttggagga aaggaataca ggggaaaaga actgatggat
     3121 tacctgggcc gtcagatcca caacgcctat tttatgccgg acggacagca ggaaaagcag
     3181 gaagcactgg atcttatgtg gtacctctgg tgcggagcca agtctcctgt gtttggtaag
     3241 gataagatga ccacatttga acattatttt gtagaagata aggcagcgca taaggaaaat
     3301 atgaatcctt actataagct cagcgagagc gaagaggtct gctgccgtat tttagaggag
     3361 ttcggcctcc cagtggaggg ctcccatatt atcaacggcc atgtgcctgt aaagctgacg
     3421 gacggtgaaa agccggtaaa agcaggcgga aagctcttta tcattgacgg agggctgtca
     3481 aaggcttatc agagcaccac aggcattgcg ggctatacgc tgatttataa ttcccatcag
     3541 ctgtcgctgg cagagcatat gccctttgat cctaaaaagg acagtacgcc caaggtgtct
     3601 atggttgaga ggatgcctcg ccggattatg gtggcagata cggacaaggg acgggagctc
     3661 aaggcacaga ttgcggattt aaaagaactg gtggcagcat accgggaagg cttaataaaa
     3721 gaaagggcag aatagatgac caaaaatgaa tttttacagg gattaaagga agagcttgag
     3781 ggacgtgtga cccattcagc cattcaggaa aatatccgat actatgatga ttatatttct
     3841 caggaggtgt taaagggaag tacggaagag gaagtgacag aaagcctggg aggccccagg
     3901 attattgcca gaaccattgt ggatgcggca tatgatacgg aggatcgtcc ggacggctat
     3961 gatgaatacg gctccggtcc ggcagaggag agtgaagcag gcaccggttc ccggagtccc
     4021 tttggcagtc aggaaaaccc ctataccgga cacagccata ttcattttct ggatttcagt
     4081 aagtggtatg tgcgcctggg cgcaggcctt gtagtatttt gtgtgatttt tcttataatg
     4141 agcgtctttt tcggcatcgt aggcctggcc ggctttatac tggcccacat ctggccggtt
     4201 cttgtgatac ttatgatcat atggatgttc agagggccga ggagatagat ggaaaagacg
     4261 aagaaaagca ggtatcagag aaagctgttt gcctatttta tggcagtagc agtgattcct
     4321 ttgtgcattt tgggaatgtt ttcctatcat acggcaaaag aggcattaag ggaaaatatt
     4381 caccatggaa atgaagctgc attggctcag attgaaaata agacagaaaa ggctctggag
     4441 atgattcgga tagactttct gggaactgcg gtttcgcctg tcacccagag gatgatcaat
     4501 agtgcttatg atgagattcc atatccggag ttaagagatt ttaaagacgg gatgggaggg
     4561 caggaaagtt acagtgatta cgtaaaaagc tatagcttta tcaatctgaa acatggctgg
     4621 attcttggaa ataagggata tatcccgctg gaggatgttc ataatcttca gtggattgaa
     4681 atcctggaga agaatcctga aaagattttc tggcttaatg aaacaggatg gaaccaggaa
     4741 ggaaaaacag atgatgtagc cagagagaca ctgacggaac agcagaagat aactccggag
     4801 tatatcagcc atgaatatct tacactggta attaaggtac ctacctattc tgaaaaaagt
     4861 gacgccatgt ttgtggtaag tctggatcag gcatatatgg aagaactatt tcggggaagt
     4921 attggaaatg gcgctctttt aattcttgat ccagaaggaa atcctgtata ttcggaggat
     4981 gaaaagctga ctgaggccta tctggatgag tcaggagaga aaaaagcgga ttatgatatt
     5041 gtaagccgta ctgccgatat gagtggctgg acttatgtgg ccggctatga ctcccggtat
     5101 gcagatggtc aaatgaatca gattatagtc acgatggcag cgctgatact gtttgtgttt
     5161 gttttgctga ttatcatgag cattctgggt gctttcagag tgtaccagcc ggtaaaggaa
     5221 ctggtatctc aggtaaagaa gattgtgccg gaacagcctc aggcagaggg ggaatttgac
     5281 ctgatccgtc aggggcttca cgcgctgacg aactccaatg aggaacttca gactatgatc
     5341 agacggcaga agagccagct cacagagctg tttgcttttc gtctgatccg gggacggatg
     5401 ggagagacag aggtccggga aagcatgggg cgtctggggc tgcagtttca gccctgtctt
     5461 tatgtagttt cggtgctttt ctgtaaaaat ggcagggatg aaaatacaga gcagatagag
     5521 caggatgtgt taaatctgga gctgatgaag cagctcccgg aggagattct tactatgctg
     5581 gccattccgc cctttatcca cacccatgcg attgtgatgg tattagacgg gccggggaag
     5641 gagaagctgg agcagaagct tttagctctg cgtaactgcc ttctggtatt tgccgggacg
     5701 gaatccggcg gctggctgaa tatgggcgtc agccgcagtt ttgaggatct gtcacatttc
     5761 cgcggagctt attttgagag tctggaggct cttaagatta acgagcactg cgaccgggat
     5821 gaggatacag agggaatctc cattgaggaa agctctgtga cttactatga agacctgatc
     5881 cgccaggagg aagaggccaa acgatacgac ctggctctgg atactgccat caaggcggcg
     5941 gtggacagct gtgaccagaa ggcagccttt gaggtagtgg atacttttgt gcgggaggtg
     6001 gaaaagagag gggtggcgct gtatgaacag cactattacc tgaatcgttt ccttttagcc
     6061 attctgactg tcccggcaga tgcaggcata ccggttcatg atctgttcgg agaggaggat
     6121 gatcccttcc gtcaggccag tcagcttttt gattatgctg ctatccgtag cttctacaaa
     6181 aagaaggtgg ttcagcctgt aatcggacgt atgaaccagt tcaggaaaag cggttctgag
     6241 atgattcttg aaaaagtcat ggctctgatc gatcagaaaa acggggatgt gacactggca
     6301 gagtgcgccc aggaattggg atatcacccc agctacatct ggagggtact gaaaaataca
     6361 agagacatta catttacaga ttatattgca gagcagaagc ttcgtattgc tacaaggctt
     6421 ttggaggaaa cggagctgtc tgtatctgag attgcggagc gtttaagcta cagcaacgca
     6481 cagaacttta tacgcctgtt taaaaagcat atgggtatta cgcctggaca gtacagaaag
     6541 cacagaaggg aaaaggcaca gtagcccttt gtcaaaagat acaaaaaaat gaccgttctc
     6601 cgggataaaa cgcacgggcg gtcatttttt ttgtgtcttc cgattctaca gatagagcat
     6661 cacatagaaa aaataggtaa aaaaaatgct cttcatcttg ccttcagata aaaacagaga
     6721 caataaggtg attatttttg tgtagaatcg gattgtttta atagaaaaat gtgcaatatg
     6781 cataaaaata cagggatgat attatgcaga aatatagaaa ctaaatgatt attgaaaaat
     6841 tagggaaatc cgctatgatg gagccatgaa agacagggcg gcagataaaa agccgttaag
     6901 attatgaaaa gggggttccg ttatggcgga taagcttgcg tatctgaagc gtcacaaatg
     6961 gatgtatttt ctgatgcttc cgggaattat ttattttatc atttttaagt atgtacccat
     7021 gggcggcctg attattgcat ttcagaatta cagcccttat cttggaattg cgggaagcga
     7081 atgggttggt tttgagcatt ttaaaaactt ctttatgaat cctgatttta agatgctttt
     7141 agtcaatacc ttttccatct cccttatgaa cctgctgttc ttcttcccgg caccgattat
     7201 cctggcactt cttttaaatg aagtgaagaa caagctttta aagcgttcca tccagaccat
     7261 gatttacatt ccgcatttta tttcaatggt aatcgttgca agtattacct ttacactctt
     7321 aaatgcggaa aacggagtga ttacacagtt gtctactatg ataacgggag agaatccgga
     7381 gtttttatcc gatcccaagt acttccgctg gattattgta ctgcagaata tatggaagga
     7441 aacaggctgg ggcatgatca ttttcctggc agccttatcc ggtgtggaca cacagctcta
     7501 tgaggcggct gagatggacg gtgccggaaa gctccgccag atgtggcaca tcacgcttcc
     7561 tgcaattaag agcactatta tcattatgct gatattaaag gtgggaacgc tgcttaacac
     7621 aggatttgaa cagattttcc tgatgaagaa ttccttaaat gcatccgttg cagaggtatt
     7681 tgatacctat gtatacacac tgggtattac gcagggagcg ttctcctaca gtacagctgt
     7741 aggtatgttt aaatctgtag tcgctacaat tatggtactg gcaacaaact ggatctgcaa
     7801 aaaatgcggt gaaaccggtt tatattaaaa agggggaaat aaaacatgac agcaaaatct
     7861 aaaacaaagc gcacaaaaat gaagcgcagc cctcaggaac ggatcggttt gtttattgta
     7921 cacgtaatct gtattacagc cggagtattg accgttgtac cgttccttta tgtactgtct
     7981 ggatcttttg ctacagaaaa ggagctgctt gagagatcct tctttattat tcctcatact
     8041 atttccacta atgcgtataa atatatcgtt gcagatggaa gtattttcag aggtctgtta
     8101 aactcaacgc ttgttactgt agtaggtacg gtggttaata tgctgtttac cactacactg
     8161 gcctatcctc tttcaaaaag ctggctcaag ggccgtaatg tcatcctgaa catggttgta
     8221 tttaccatgc tgttctccgg cggtatgatt ccaaactatc tggttgtcaa gggactggat
     8281 cttctggatt cttactgggc cttaatcctt ccgggagcaa tcagtgcctt taacctgatt
     8341 attgtaaaga acttcttcca ggagcttccg ggagagttag aagaggcggc caagattgat
     8401 ggctgttcag atctgggaat tttcaccaaa atcgtacttc ccttatccaa accggctctg
     8461 gcttctgtgg gactgtttta tgcggtatca cactggaatg acttctttaa ttccatgatc
     8521 tacttaaaca gtacggagaa atttccggtt cagattattc tgcgtcagat cgttcttctg
     8581 gcacaggggg ccagcgcaga tggaagcgca attgacttcg gagcaggcgg aacacctccg
     8641 gagcaggcag taaagatggc agctactgta gtagcatgtc tccctatttt atgtgtatat
     8701 ccctttgttc agagatattt tgaacagggc gttatggtag gcgctgtaaa gggttaatga
     8761 aaaatttatg gaggtagcaa aatgaaaaaa tcaacttgga aaaaaggctt gtcagtaacg
     8821 ctggcagccg caatgacggc tggtctggca gcaggctgcg gaagcaaaaa ggaagcatct
     8881 tccggcacgg cagaggatgg aaagtacaat ctgaagatca tgagctatga cttctttgga
     8941 aatcccatga agggtgatgt gggggcaggc atcatgaagc aggtggagga ctatacagga
     9001 acccatttgg acatcatgtg gacgccaaag gataactatg acgataagct gaacctggtg
     9061 ctggctggcg gtggcgatga tatgccccag attattgcca cagatacaaa gtctccggcg
     9121 attatcaacg ctgcaagagc aggcgctctg tgggatgttg aggagctgat gaaggaattc
     9181 cctcatctta aaaatgcaaa gcctgaggta aatgacaaca tccgtatcga cggaaagctt
     9241 tacggcgtat acagaggacg tgctctggga agaaacggct tgaccttccg caaggactgg
     9301 gccgacaatc tgggcctgga agctcctgaa accattgagg atgtttacaa tatgcttcat
     9361 gcatttacat acgatgatcc ggacggaaac ggacaggatg atacttacgg tctggctctg
     9421 tccaagtcaa cggctcccat tgatattatc cagacatggt tcggcgctcc caacggctgg
     9481 ggtgaaaagg acggaaaact ggttccggag catcagacag aggagtatct ggaagcatta
     9541 aactggatga gaaagctttg tgaggaagga cttattaaaa aggatttccc taccagagat
     9601 attgctacca agtcagatga tttaaagaca cagaaggccg gtatgatggt agatgctctg
     9661 gatgacggac gccgtgtgca ggattacttt gaaaatcagg ggattgaagg acctgagatt
     9721 aattttgtag gagccatcaa aaaggatgag gcatctgaac ccagaacggg agcaacgctt
     9781 ggctgtcagg gctttttcgt aatcactaag gcagctaaga cagaagagga tgtacgtaaa
     9841 tgtctggatt tcctggacaa gatgaatgat gaggaaatgc ttacattggc taactatggt
     9901 ctggagggca accatcacac gattgaagca gatggaaggc ttacacgaag tcatgacgcg
     9961 accttaaatc aggaatatca ggcgttaaat cagctggtgt cctataccga gtaccctccg
    10021 aacatggatc catatgtaga actgaatgag acagagtatt actatatgca gcaggatacc
    10081 attgcagcca atgaacagta tgcagtttct gaccccgcag ccggaattct gggcgattct
    10141 gaggaataca tcaagaacgg tgttgctctg gacaagatca ttgaggatgc aagagttcag
    10201 tatatcgtag gacagatcga tgagacacag ttaaaggcac agtgggatct gtggtctcag
    10261 agcggtggcg acaaggtaat cgaagaagta aatgcaaagt acgcagaatt aaaaaataag
    10321 taacaggaaa gcttttgaag ggcgcggaaa cagactgggt gataaaatcg gatccggctg
    10381 gtttccgggc ccttttccga gatatgccgc agggaaagcg gcgaacagga ggaaataaca
    10441 atggtatcag aaaaagcatt tgcaaatccg ccggtgagca gccggattaa gcccttctgg
    10501 ttctggaacg gaaccatgac aagggaggaa atcagccatc agatcaaaga aatggcagac
    10561 aagggtctgg gaggtatgtt tatctgcgcc agacagggaa tggatgtgcc gtatctttct
    10621 ggtaaatggt ttgagctggt ggattttgcc tgcagagaag caaaacggta tggcctggag
    10681 gcctggttat atgacgaata tccttatccc agcggtatga gcggcggcga ggtgctgctg
    10741 gagcatccgg aggcagagca taagatatta aaacataaga agtttcggac agagggaaaa
    10801 atacgtgtga cagaggagct gggctggagc cggattttgt atgcaaaggc atttccggtg
    10861 gatgagaatg gaaagacaat ctgggaaaat cctctggatt tagagtcctc tgtgggaatc
    10921 ctgcagacag aaaaaatcta ccagcagacc ggcctgaccc ggtataataa taagcgtttc
    10981 ttttcctatg gtcctaaaaa aattctggat gcagtgctgc ctgagggcca gtgggagatt
    11041 cagatatgta cggaagaggc tatgggggat tttaagtatt acggaggatt ttttgacccc
    11101 tgtaaccggg aggcagtaaa gacgttcctt gatacgactc atgaacggta cgcaaaaaca
    11161 aatggggaag aattcggcgt cagcgtattt ggcatgtttt ctgatgaagt gggtctttta
    11221 agccccattc cctggtcctc caggcttcct gaggctttta aggtccgcaa cgggtattct
    11281 attttagagg tgcttccggg catttatgag ccggattatc cgggagcggc aaagctacga
    11341 tatgacctgt atgaaacggc tcatgagctt ttcagggaga gctatcatca tcaggtcgca
    11401 tcctggtgtg aggagcatca tctgctgtat gccacagagg tgccgtccat gcgccacagc
    11461 acccagaagt tcagccatat tccaggaggt gacacagccc atgaaaagct tggaaaatct
    11521 ctggaatgga tttatgatga atatatccgc aactatcgca gcaacgccaa ggcagtaagc
    11581 tctctggccc gtcagttaga tcggaagtac gctatgatcg agagcttcca cagcgttggc
    11641 tggaccatga atatgcagga tgcaaagtgg atgattgacc gtctgggaag cagcggcatc
    11701 aatctctaca atttccatgc cttttattat accattcagg atattacaaa gcatgacgct
    11761 cctccttccc agtttctcca gaatccttac tggaaggatt acaggaagct ggcagactat
    11821 gtgggaagaa tgggagagat cattaccaac acagaggcag atatccatat agcagtgctg
    11881 gatccaatcg cagccatgtg gactcttctt ggaaatccct tccagggctt tgtatacaaa
    11941 ggcgagtcgg aggaagaaaa ggcagcctgt gatcggatcc gggacggctg ggtggctgtg
    12001 tgcaaggagc ttttgtttgc acagctggat tatgaccatc tggacggcga gcttttagaa
    12061 gaggcagaaa tctgtgatgg aagtatccgt atgggaagag cggcctattc ggtggtggtt
    12121 cttccggaat gccactgcat ggagaaaaag accagagaaa tgctggagcg gtttgttcag
    12181 gcaggaggcc atgtgatcgc agtgggagag cttccttccg tttctattga taaggaggaa
    12241 acagatcagg agacagaggc agcctggaag gcgcttacgg cccatgcaaa tgtagtttgc
    12301 ctgaatggaa gcagagaggc gcttcctgac tgctgcagag gctatatcag ggaagcaggc
    12361 cgtgtggagg ttctggaggg agacagaaaa tccgtcatcg gtgtgtgcag acaggatgag
    12421 gcaggttctg tttacctgtt tgcagccaat cagggcgcag agcctgtgaa ggtgagattc
    12481 agcagtgaaa atggaacaaa cagtgccgtg tggatggatc tggaaaaggg gcggcagatt
    12541 ccggcagaaa atccggaaaa taagggaatt gccgtcactc tggagggctt tgagagccgg
    12601 tggctgaagc tctctgattt tgaggaggaa gcagtgggaa gagagccgga aggcactttg
    12661 ggaaattcct gtctttccct ggttcaggct tctatgatgg atgaaggcgg caggagccgg
    12721 tgtgcaaagc atatttccat ggcaggaaag tggaaatgct ttcctcagag tttgaatgtg
    12781 tgcagattcg gagaaaccga ggtttcggta gacggaacaa attgggaaaa agtggaagta
    12841 aaaactttta ttgagcagtg tgcccagacg aagaggcttg gcggagaaca gatcgccatg
    12901 gaaggggaat ttggaacacc cagaaagctt agacccgctt atcccattac ctgtttctat
    12961 aaagtgggat ttaagataag gaagggcgag ctgccttctg agctgttcct tcttatggat
    13021 cgggagacca tcagcggaag caatgccata gagatcaacg gaagggttgt ggaggcgtct
    13081 ctctggaagc cggtgcgggt aaatgatttt aacaatcagg cagcggacat ccgttccttg
    13141 gtgcgggagg gtgaaaatga gatccgtatc cgggtctgca ttcggaagga tgaggacggc
    13201 ctgagagatc ccttctatct gtggggaagc tttggcgttg gttttgagga tggctgtcat
    13261 gtgcttaccg cccagcctga gcaggtatgc ccggatgctc cttggatcca gggcttccca
    13321 tattacagcg gaaccatgga atttgaaaca gagattgaat ttgatagcag cgaggcctgt
    13381 attcttgccc ttgactgggc gtatccctgc catgagaaca tagaggtact ggtaaatgga
    13441 aggagtaccg gaatctgtgc ctacagccct tatctctggg aatgtgggca gggaacggca
    13501 aagcagggaa tgaactgcgt gacggttcgt atttccaata cactggctaa tatgctggat
    13561 ggaacgtatt ttgactataa ggaacaccgc cttgcggata tcagacagga ggcagaagag
    13621 cgatgacaac gtattttgac agagagcaat cgattgagta ccgtcttggc agtgaaacgg
    13681 ggcctgtttt gcagaccata gccaaccgtt tctgcgccga caatccggct gtgcccgtgc
    13741 agtacagttt aagaagcaca cagggatttt ccattacgaa ggattatctg gacgaaattc
    13801 ttctccatga aaaatttcca cagatggaac gggagcagta tgtatacgcc agagcggggc
    13861 ttacgcccca ggcacccggc ccctttcatt ttcgcttcgg cgcatatggc cctgtgacca
    13921 tctatgtaaa cggaaaaaaa tcatttcaga ccaatcatac ccaggagcgc ttttccgaga
    13981 cgctttccga tatctgtcta gatttaaagg agggggtaaa tgaccttctc tttgaatgtg
    14041 tcagcactcc cttaggctgc ggattccgta tcgggagcag ctcctataag ggccgcagga
    14101 tccagttttt tgcagtagat gaagcccata agggaatgaa tggatttgtc tatacagagc
    14161 cccaaaagac tgccatggat cctgcagatg cagattccgt atgctggctt ccgagagctg
    14221 tatggacgga agaagagaaa caggaatcgg tctgcagccg gctttttgac ggcaaaacca
    14281 tgctgtcggt gacaagggtt tttgtgcctc aggagggaat gatacggatc cttggagcct
    14341 gcgggagaac aggacaggag ccggtggagg tctttgcaga cggaaagcgg gcgggaacca
    14401 gtaaggacga ttctctaacg cttgagcttt cgctggcaag cggataccat accctggcgt
    14461 ttctgggagg agacccggat gtgacagtgg aggacaggca ggggacggtt ctggaattgg
    14521 aagctccggt tttatgtatg ggacagcccc agacctggat ttatgcaggt ccttttgacc
    14581 gggtacgccc ttccgaggag gagatttcca ggatgctttc ctttaaaaag ccctttgaaa
    14641 ccgatctggg aaaggaattc tggcgggtgg atgggccaga tctgtatctt cgcccggtca
    14701 atgagggaac gctttacgga gagtggaatt accctctggg cgtgaccctt tacggaatga
    14761 tacagaccgc aagatatgaa catgataatg ctctggagtg ttatgtccgg gggcatatgg
    14821 agaaatgcac cggattttat gagtactgta tgtgggataa agagcactac ggagcagctc
    14881 ccttccacaa tcaactcaca accattgaca gcctggatga ctgtggctcc tttgcttccg
    14941 ccctgctgga ggtaatgaag gattatgaga tcccggaggg ggatgttatc agcgccatgg
    15001 tggcggatta tatgaaggac aggcagcaga ggatggagga cggtaccttt tacagaaacc
    15061 attcttacct gcctgttatg aatgaaacca tctgggctga tgatctgtat atgagcgttc
    15121 ctttcctgtg ccgctactat aagcgcagtg gggatgaatg ctggttaaag gaagcggcgg
    15181 gacagttaaa gcgcattttt ggctatctgt atatgccgga ggaaggggtt ctctcccata
    15241 tctatgatac tcactacggg gttcagacaa aggttccctg gggcagagga aacggctggg
    15301 ctctcttctc cgctgctgaa ctgctttctg tgatgccaaa ggagcatgag aacagagagg
    15361 agattctgga tatttaccgg acgctgtgcc ggggatattt aaaggtacag gatccggacg
    15421 gaatgtggca tcaggtgctt accatgaagg aatcctatga ggaaacctcc tgcacggcca
    15481 tgtttatata cggctttgca aagggcgtaa aaaatggctg gcatacagac ggggaggcat
    15541 acagaaaggc ggccataaag ggatggaggg cgctgtgccg tacctccatt gactggaagg
    15601 gaaatatcta cggcgtatgc cggggctccg gctattcttt cagcagagag tattatgctt
    15661 atgagctggg ctggaatttt aatgataccc acggcacggg cattgtgctc ttagccggaa
    15721 tcgagacaga gcgtatgttg tgtccggaag agtaagagga ggaaagagaa catggaacag
    15781 atatttgatc ccagagaatt tggtgcggca ggagacggaa agacgctgga cacacaggct
    15841 attcaggctg ccattgatgc ggctgccaaa gagggaagag acggaaaacg gtctgttgtc
    15901 tgcctgaagg caggaaccta tcttaccagc tctctgtttt taaagagcca tatggaattt
    15961 cggatggagg acgcagctgt gcttttaggc accacagagg aggagcagta tccgattatg
    16021 agaaaccggg tggccggaat agagatggac tggccggtgg gaatcctcaa tgtgatggat
    16081 caggaggatg tgcagatcac gggagaaggc tgtatcgacg gacagggccc ctactggtgg
    16141 aataaatact ggggagagga tcagaaaggc ggtatgcgtg ccgagtatga tcctatgggc
    16201 ctgcgatggt gcgtggatta tgactgcaga cgagtgagaa acctggtggt aatggacagc
    16261 cggaggatcg aaattgcagg aatcggaagc cggcgctcag gcttttggaa tatgcacatc
    16321 tgttattcag aagatgtgca tgtggacggt gtctggatcc gggataatga ggggcccagc
    16381 acagacggaa tcgacattga ctcctgccgc catgtagtag tagagaactg ccgggtggcc
    16441 tgcaatgatg acagcatctg cgtaaaatca ggaagggatg cagacggact cagggtaaac
    16501 cgtatctgtg aggacgtact aattcagaac tgccaggtac tcacaggctg cggagtaacc
    16561 cttggaagtg agacctccgg cggcatccgc aatgtgacga tccgcaatat gaagtatcac
    16621 ggcacagact gcggcttccg catcaaatcc gctgccacca gaggcggcgt gatggaggat
    16681 atcctggtag aagatctgga gatggtaaat gtaaaatatc ccatcaatat gtgcctgaac
    16741 tggcacccgg cttacagcta ctgcgaaatc ccgaagggct atgaggggga aatcccggag
    16801 cactggaagg tactggcaca gtctgtttcc agagaaatgg gcgttcccca ggtgaaaaac
    16861 ctgcagatcc gcaatgtgcg ctcctggaat gaggagggct atgagggctg ttcccgtgcc
    16921 tttgaggtag acgctttccc ggaacgccct atggagaatg tagtgctgga gcatgtggat
    16981 ataaaggcga aggaatttgg ccgtattgcg ggaattaaga actggcagtg gaaggatgta
    17041 aacatttctg tggagggaag aaaccaggat gagaataatg tgtatgatgt gaggtaattc
    17101 caccattttc cattgacaaa tcctgacaga aagaatatac tttattacgt tgtgtaacaa
    17161 ttttgtaaca attacagccg attcaggtat tctaaaagtc aggggagtta agatttgaag
    17221 ctggatgtat ttcgcaggct tgcagtgaag gctgcagcag gagtatttat tttcgcagga
    17281 cttatgatga tggatccgca ggagagcatg gcggcggcca ggacagaggt gcagacccga
    17341 tcctcttata tggtgaccat tgaggctcca tctgtggatg tatatacata taaaaacaag
    17401 gattccaaaa ccgtaggtca ggtaaaaaga ggacagactt atgaggtgct taaaaaggaa
    17461 caggacggct gggtacagat ccgcatggga aaccgggagg gatacatttc tgcggcagga
    17521 aatgcctctc tggtagaaaa gaaccgtcag actgtggaca gcggggccag acagcggcgg
    17581 gaagtagtgg aatttgctct tcagtttctg ggaggcgaat ataaatacgg cggaaccgat
    17641 ccgaacaagg gagtagactg ctccggtttt acacgatatg tcatgaagta tggcgcttct
    17701 gtggagattc ctcattcttc cacaggacag gctgcctgcg gaacggaagt ttccgaagaa
    17761 gagatgcagc caggagacct gatcttttac agcagtccct ttggcaggat caatcatgtg
    17821 gctatgtatg cgggaaacgg gcaggttgtt catgcttcta cagaagaaac aggcattaag
    17881 ctgtctcctt ataattaccg ggagcctgtg aagattgtaa gtgttttgga gtaaatctta
    17941 aaaaaatata aacaggttga cgaaaatctt atgtatgtta taataaaatc gtaatgaacg
    18001 taacaaatat gtaacaattc aaatcggatt tgtaataaac ggggatgacg aagaacatgc
    18061 ataatttttt caggaaactg gttggaacag gtgttgtgtg cgggatgctg gtatttgcgg
    18121 ctcctcttac ttctatggca gccattgggc cggggtttgc agcaggtacg tacgtggcga
    18181 ccgtgacagc ggaaagcgta aatatcaata agaaccgcga cagcgaggag gttcttttta
    18241 cagcgaaggc cggcagtaca tatgaagtat tagaggactg cggagacggc tggatgaagg
    18301 ttcgggttca cgatacagaa ggatatcttc cggtttctga aaatgcagtg gtggaagagg
    18361 cagaggaagg cgagatcgcc atgatccaga aagaggccag ggagtcatcc gcttcctata
    18421 agagacagca gcttgcagac tatgccctgc agtttgtagg aggcccctat caatacggag
    18481 ggagcgatcc tcatacaggt gtggactgct ccggctttac cagatatgtg tatcagcacg
    18541 gtgcaggcat tacattaaac cgttcttcca gaggacaggc tcttcaggga aaagaaatca
    18601 gtgcagatca gatgcgtccg ggagatcttt tgttttacgg aagcaaaagc aacattgacc
    18661 atgttgccat gtatattgga gaaggaaaga tcgtccacgc ttctacggag cgcacaggca
    18721 ttacgatttc caactggaat tacagaaatc cggtaaagat cagaagtatt atggagtaga
    18781 ttacggggga aacccacgca cggttcggat tccgtgcaaa ggattaggat aaaaagatag
    18841 agagaaaggc cgggataggg gagctatcct ggccttttct caaaggggag tataaataat
    18901 taataaggat gagggtgcag cttaagggaa actgcattac ctcatactca tttataatac
    18961 aaaaatttca aaaaagcaac cgctttgagg aatttgtata aaaccacagt tttccgccat
    19021 actagagggt gtatcgtaaa ccatcccggc ggcggaaatt gtttcgcctg tcaggaaaat
    19081 catttcagga ggtatttgcc atgtcaagca gaaatgacaa catgaacagc tacagcaaca
    19141 gccagaataa cgcacagaac aacagccgaa ataactcttc aaacaacgca tcaaacggcc
    19201 gttctaaaaa taacatgaag aattccgctg agaataagta cgagaactgc ggaagaaaca
    19261 acgcaagaaa cgattctaac gactgatctt tgagatcaga aagataggca tccgcataaa
    19321 aatggcatct gcagagagaa ctctttttgc agatgccgtt tttttaggct tcttcgtgag
    19381 gaacctgtcc ggagcagacg catatactac agtaggacac gccacagaca gaaggaaaac
    19441 agaaagcggg gtggagtgat gaatgatttt ccgatgatct cgtacaaaca gcttgatcaa
    19501 tggctggagc agggaaatgt ccgcctcctg gcagatgtga gagaaccgtg gatgtatggt
    19561 gagaaccgca ttttgggcag tgtgaatata ccatatgaag aggtaagaga acgtttggaa
    19621 gagtttccca gacagggcat gatcgtatgt tactgcgacc gcggcgccaa gagcatggtg
    19681 gtctgcagag acctctggcg cctgggatat gaggtgtctg atctggcagg aggaatgttg
    19741 gattacaggg ggaaatacgt tgacagaaga cctctgtggg atttaaaata gaggaaacac
    19801 ttcaggacgg cgtgaaaaca ggcgggagcc cgtgcgtcaa gctcgcttgt aagcacgggt
    19861 tcccgcctgt tttcacatcg gatgaacatt cgatgcttct tgtccggccc acagccggac
    19921 aagaagcccc atcctgaaat gcctcacctt attactaaaa aagaaaggaa ccatcatctc
    19981 atgaaattat tattcgcatc tgatattcac ggctctgctt tttactgcag gagactgtta
    20041 gatatttata aagaatccgg tgcctcccgg ctggtgctgc tgggagacat tctgtatcat
    20101 ggcccaagaa atgaccttcc aaaggaatat gctcccaaag aagtcattgc catgctgaat
    20161 ccattaaaga accagatcta tgctgttcgg ggaaactgtg atacggaggt tgatcagatg
    20221 gttctggaat tccccattct ggcagattat gccctttttt ctgtggaggg aaagaccctt
    20281 tatgctaccc acggacacat ttttaatcag gacaatcttc cgcctatgca ggaaggtgat
    20341 attctcatac acggccatac ccatctgtta aaggctgagg agacagaggg agactgcggc
    20401 aggatcaggg tgttaaatcc cggttccgtg tccattccca agggaggaaa tccgcataca
    20461 tatggtattt tggaggacgg cgtatttacc atccgtacac tggacggtga agcggttaag
    20521 gaaatgaaat tgtaaaagag aggagaacca gttgaagcag acctatgaac tgatcgcccc
    20581 ctgccatttc ggcctggagg cagtactaaa aaaagaaatc ctggatctgg gatatgagat
    20641 cgcttctgta gaagatggaa gagtaacctt tatcggagat gacgaggcaa tctgcagagc
    20701 caatgtattt ttaaggacag cagaacgcat tttattgaaa gcaggcagct ttaaggcaga
    20761 aacctttgaa gaactgtttc agggcaccag agccattgca tgggaggaac tgattccgga
    20821 agatggaaaa ttctgggtgg caaaggcgtc ttccatcaag agcaagctgt tcagcccctc
    20881 cgatattcag tctattatga aaaaagccat ggtagagcgg atgaagagcc attataaggt
    20941 gacttggttc ccggaaaatg gagccagctt ccctctgagg gtttttctgt acaaggatat
    21001 tgtaaccatt ggaattgaca ccagcggaga ttcccttcac aaaagggggt atcgtactct
    21061 taccagcaag gcaccaatta cggaaacact ggcgtcggcc ctgatccttt taacaccctg
    21121 gaataaggat cggatcctgg tagatccttt ctgcggcagc ggcaccttcc ctattgaggc
    21181 tgctatgatc gccgccaata tagctccggg catgaaccgg tctttcctgg cagaggactg
    21241 gaaaaatatt atcgggcgca aatgctggta tgaggctatg gatgaggcaa acgatctggt
    21301 ggatgacagc gtgcaggtcg atattcaggg ctatgatatt gacggggata tcgtaaaggc
    21361 cgcccgctta aatgcccagt ctgcaggcgt ggaccatatg atccatttcc agcagcggcc
    21421 tgtaagcgct ttaagccatc cgaaaaaata tggctttatt atttctaatc ctccttacgg
    21481 agagcggatt gaagagaaga agaatcttcc ggttctctac agggaaatcg gagagcggtt
    21541 tgccgctctg gacgcctggt ctatgtacct gatcacatcc tatgaggata cggaaaaatg
    21601 cattgggcgc aaggcggata agaaccgaaa gatctataac ggcatgctga aaacctattt
    21661 ctatcagttt atggggccga agcctcccag acgcagtcag gagaagaaaa ttgataaaca
    21721 ggaataggag ggctttgggg gctgttttgg ccgtttgcat agctgttatg acggcaggct
    21781 gcgctgtttt ggcagataaa aggagtccag atagttcgtt cggatatgaa gatgtccgcg
    21841 ggcaggaaac agaacaaacg gagcagagcc tttctgccct tccggaggtg ggagagaagg
    21901 atgcttcctg ccagatttgg aaccgggggc ttcctgagga tacaattctg ccccgttcct
    21961 atgattacag ggaggaagga cgtgcgcccc agattggaaa tcagggctct cttggaacct
    22021 gctgggcctt tgcctccctg acggcactgg aatcgtcgct tcttcctgag aagaatgagt
    22081 cattttcagt ggatcatatg tccatgcata ataattatac gctgggacag gatgaaggcg
    22141 gagaatatac catgtccatg gcatatcttc tgggctggca gggacccgtg tgggaatccg
    22201 aggaccctta tggagacggc gtttccccct gcggcctgaa gcccaggctt catgtacagg
    22261 aaatccagat ccttccgtct aaggattatg aggcgatcaa gagggctgta tatctggagg
    22321 gaggagttca aagttccctt tatacttcca tgcaggatta tgacagtgaa tccgtgtatt
    22381 acaaccggga tacgaattcc tactgctatg tgggaacgaa cgagccgaac catgattctg
    22441 ttatcattgg ctgggatgat gattatccaa aagaaaattt caatatggat ctggagggtg
    22501 acggagcatt tatctgtacc aacagctggg gagaaaactt tggcgatgaa ggctattttt
    22561 atgtgtccta ttacgatacc aatatcggcg ttcacaacat tgtatacacc ggggcagagt
    22621 ccgccgggct ttatgaaaag atctatcaga gcgacctgtg cggctgggta gggcagatcg
    22681 gctatggtca ggataccgtc tgggcgacca ataccttccg ggcaggtgag aaggaagaac
    22741 tggaagccgc cggattttat gcgaccggac ccgatacgga gtatgaaatg tatctggcca
    22801 gatatctgcc ggaagtacag gaggggattt ccggggctga aaaggaaagc cagagcgccc
    22861 gtatggatcg ggctttgaat gaccgaaggc ccatagccag gggaaaactg atgtattccg
    22921 gctattatac ggttcctttt gatgaaaaaa tactattgga tgcaggagaa aaatttgcta
    22981 taatagttaa aataaaaact ccggataccg ttcatccggc agcgatcgaa tatgatgccg
    23041 gagacgggat cgccagagtc gatcttacag acggagaagg atatctgagc catgacggca
    23101 gtctctggga gcgtgtggag gaaactcaga aatgcaacct ctgcctgaag gcatatacca
    23161 atacaaaagg atagaggatt atggaacaga agatcatttt tgcaacaggg aatcagggaa
    23221 aaatgaggga gatccgggcc attttgtccg atctggggct tcctgtgctg tccatgaagg
    23281 aggcaggagt ttctctggat attacagaag atggaacgtc ttttgccgaa aatgcccgta
    23341 tcaaggccag agctgtctgg gagcatacag gcgggatcgt attggcagat gattccggtc
    23401 tggcagtgga ttatatcggc ggcgagccgg gcgtttattc tgcgcggtat atgggagagg
    23461 atacctccta tgagattaaa aacagaaata tcatagaccg gcttttgggg gcagagggaa
    23521 aggagcggag cgcccgcttt gtctgcaata ttgcagctgt gctgcccgat ggacgagtga
    23581 tccagacaga ggcatatatg gaaggtgtga tcgcaaagga acctgccgga acgggcggct
    23641 ttggttatga tcctatcctg tatcttcctg aatttggaaa aacctctgcg gaacttacca
    23701 tggaggagaa aaacaggatc agccacagag gaaaggcatt agaggccatg aagagcgcgc
    23761 tgaagcagga gattgcggtg acaggtggga gaatggcgta agataaagga aggaaacagg
    23821 ggatgaaaat actgattgtc agtgatacgc acagaaaaga tggaaatctc cagtgggtca
    23881 tggaaaagga aaagcccttt gatatgctga tccatctggg agatgccgaa ggaagcgaac
    23941 agtacatcgc tcattgggcc gaaccgggtt gtgaactgga gatggttctg ggaaacaatg
    24001 acttcttttc ccatctggag cgggaacggg atcttatgat cggaaaatac aaaacgcttt
    24061 taacccatgg ccattactat catgtgtctt taagcacaga gtatttaaaa gaagaggcaa
    24121 ggtcaagaga ttttgatatc gctatgttcg gtcacaccca ccggcctttt tttgagattg
    24181 accggaagaa gggacaaaaa gatctgatcg tgcttaatcc cggaagcctg tcttacccca
    24241 ggcaggaggg caggcggccg tcctatatga tcatggagat cggggaggac ggagaggcgg
    24301 catttaacca ctattatctg taaacaggag agaactgctt tttagggagc ggttcttttt
    24361 tgtagaaatg atagtaaggg cggctgcttc cacacgaaca ggtatttgct tgcagtcagg
    24421 gaacagcgtc tgaaaaaaag gaatattttt aagagttgac gatacaatac ttgtgtgata
    24481 tgattaaaaa aataaaagaa atggaacgtg aatagttgaa ttttgacaag gcttatatat
    24541 gctcataatt ggttgagcgt ttctaccaac taccgcaaat agttgtctat aagttttcac
    24601 agcgggagtg tctgtgaaag catgaaggca gcggtggttt ttttgtgcgg atagagagta
    24661 aaaatctgga aaatggtgtt tcagagcgca gaaaggaggc acagagcttc atggaaggaa
    24721 agagcagcag acaggacagc agctccttaa gagagcggat tctggcggct tcgggtgaaa
    24781 aaagggcagg tctggtgtta aagggcgcca gggtagtgaa tgtatttacc ggggagctgg
    24841 aggaagccga tgtggcaata gaaggtggat acatcgcagg agttggttcc tatgagggag
    24901 aaacggaggt ggatctgtca ggaaagatta tctgcccagg ctttatagac ggccacattc
    24961 atttggaaag ttctatggta aggccggctg agtttgaacg ggcggttctg ccccacggca
    25021 ccacggcagt gattacagat ccccacgaga ttgccaatgt ggcaggcaca gagggaatcg
    25081 attacatgct ttatgaaaca gaaaatctga tgctggatgt gttttttatg cttccatcct
    25141 gcgtaccggc agccgattta gaggagtccg gggctgtgct ccgtgcagag gatctgagag
    25201 cctactatga aaatcccagg gtgctggggc tggcagagat gatgaatgct cccggtactg
    25261 taaaggcaga ggaaggcgtt ctggctaagc ttaaggactg ttcggacagg ggaaaacgga
    25321 ttgacggcca tgctcccgga ctttcaggat atcagttaaa tgcttactgc gctgcgggag
    25381 tggcttcgga tcacgaatgc tccgaagcag aggaagcgct ggaaaagctg cggcggggac
    25441 agtggatcat gatccgcgag ggaacagcag ccagaaatct ggaaggactt atttcattat
    25501 gcaagcagcc ttatgtaaac cgatgtatgt ttgttacgga tgataagcat ccgggagatc
    25561 tgcagagaaa gggccatatg gatgggatca tccgaaaagc agtccggctg ggagcagatc
    25621 cggtgacagc gattcagatg gcaacgcttc atccggcccg gtattttggt cttcgtgaaa
    25681 atggggcagt agcgccgggc tatcgggcag atctggtggt tttagaggat ctggagcaga
    25741 taaaagcagt gcaggtctat aaaaacggga agctggcggc gcagatggga gagctgatct
    25801 cacaagagaa gcgcagagag aaatccgaaa gcccgtcctt tgaccgcgtg catcattcct
    25861 tccatatgaa ggaagtttgt gagcaggatt tcaggctgga gccggagggg gcctactgtc
    25921 gggtgattga gcttacacca ggagaacttt tgacaaagga gcggaatgca gcctgggtac
    25981 agcttcctgg ctttgctccc ggggtaaatc ctcaggagga tattataaag ctggctgttc
    26041 tggaacgcca taaaggaacc ggacataagg ggattggatt tctgggaggc tatggcttaa
    26101 aggccggagc agtggcgacc agtgtagcgc atgattccca taacctcatt gtagccggcg
    26161 tcagtgaccg ggatatggct ctggccgcgg agacggtgag aaaggcgcag ggagggattg
    26221 ccgtagtaaa ggacggagcg gtactggggc ttcttcctct tccaatcggc ggactgatga
    26281 acccaatggc agcagaagca gtagatgaaa agctggagga acttaagaga ctggcggccg
    26341 gtctgggtgt cagagagggg atcgatccct ttatgactct ggcatttgta agccttcccg
    26401 taattccaaa gctgcgttta aatacctgtg gcctgataga tacacagctg caggaaatcc
    26461 gggctgtttc tttttcataa aagagcggca aaacggaaca tggaatgaaa aagtaggagg
    26521 aaacttgtat ggatctggag aaaatatttc acctgaagga aaataagaca gatgtaaaaa
    26581 cagaggtcat cgcaggaatt accactttta tgacaatggc ctacattctg gcagttaatc
    26641 ccagtgtact gagcgcctgt ggaatggaca gcggcgctgt atttaccgct accgctctgg
    26701 cggctcttat cgcgacgctc ttaatggcgg cattttccaa ctaccccttt gtacttgctc
    26761 ccggtatggg cttaaatgcg tatttttcct ataccgttgt tttgcagatg gggtatagct
    26821 ggcagaccgc tctggcagcc gtatttattg aaggtatcgt atttatcctt ctgtctttga
    26881 caaatgtacg cgaggcgatt ttcaatgcca tccctatgaa tttaaaacat gcggtatctg
    26941 tgggtatcgg acttttcatt gcatttatcg gcctgcagaa tgcgcatatt gtagtagaca
    27001 gcgcgacttt ggtaggcgtg tattctttta aaagttccgt agaaaatggt ctgtttaaca
    27061 gtgaagggat cacagtcgtt ctggctctgg tgggtattct ggttacaggt attctggttg
    27121 taaagggcgt aaaaggaaac atcctctggg gtatcctgat cacatggggt cttggcattc
    27181 tctgccagct tcttggaatc tatcaggtaa atcctgagat tggcatgtac agtctgctgc
    27241 ctgatttttc caatggaatc agtgttccca gtctggcgcc taccttaatg cagatggact
    27301 tttccggtat tttatccctg gatttctttg tagtcatgtt tgcattcctg ttcgtagacc
    27361 tgtttgatac ccttggaacc ctcattggcg tggcttccaa ggcagatatg ctggataagg
    27421 atggaaagct cccaaggatc cgcggcgccc tgttatccga cgccatcgga acctctgtag
    27481 gcgctatgct tggtacctcc accactacca tatttgtaga gagtgcatcc ggcgtggcag
    27541 agggcggacg tacgggactt accgcagttg tagctgcaat cctttttgga ctgtccctgt
    27601 tcttgtcccc gattttcctg gccatcccgt cctttgcaac ggctccggct ctggtagtag
    27661 taggctttct gatgctgacc tctattacaa agattaactt tgatgacttc acagaagcta
    27721 ttcccgcata cgttgccatc atcgcaatgc ccttcatgta cagcatttcc gaaggaattg
    27781 caatgggcgt tatctcctac gttgtaatca atgtagtgac cggaaaaatg aaggaaaaga
    27841 agatcagccc gctgatgtat gtgctggcgg tgctgtttat cgggaagtac attatgctgt
    27901 aagatacata aatttatagt tcagttgcag cactgcaaaa acaggcggga aaggaggccg
    27961 cagaccttac ccgcctgttt ttgcgtgaat ttagtaacag ttcagactag ccatagataa
    28021 acgtgagaaa ttgaaaaaag attttttctt tttggtttat ttctggtaaa gatgagggaa
    28081 ttttgaaaaa acagttgaca attccctcat cctgttttat aatacctctt gttcgtgcag
    28141 gtgtagttca atggtagaac actagccttc caagctagat acgtgggttc gattc
//
LOCUS       DBMDUH010000014        28142 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_1212_length_28142_cov_6.849435, whole genome shotgun sequence.
ACCESSION   DBMDUH010000014 DBMDUH010000000
VERSION     DBMDUH010000014.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 28142)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 28142)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28142
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1212_length_28142_cov_6.849435"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(1..176)
                     /locus_tag="ACZPCG_02535"
                     /pseudo
     CDS             complement(1..176)
                     /locus_tag="ACZPCG_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron maturase-specific
                     domain-containing protein"
     gene            complement(393..1160)
                     /locus_tag="ACZPCG_02540"
     CDS             complement(393..1160)
                     /locus_tag="ACZPCG_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1283983.1"
                     /translation="MMSFLETGRALYLLAAICLGGIMVRLAAGSFYKRLIRESSNLAL
                     AKNRYLRALRQNAEDTYRINMGMNNTRVYLERQIYSLRFMGISLQGFENLGGQLTLLC
                     FLAGGAAAFLSYWNRSDNYYIVLYGVMGIFGGMATVFVDYGVNLEAKRQHLLTCLQDH
                     VENVMWSRMERETASDQQPAPAAEEERREAAPVRSIGRDRRVHARRSAEQTAASRETL
                     REVSRDAREDRSGENWLQDLNPDQKRMLGEMLKGFIS"
     gene            complement(1381..2073)
                     /locus_tag="ACZPCG_02545"
     CDS             complement(1381..2073)
                     /locus_tag="ACZPCG_02545"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HIR1283984.1"
                     /translation="MIAIIGAMDEEVAMLKEKMTEVQVESRACMEFYKGKLEGKDTVV
                     VRSGIGKVNAAMCAQILADRYAPSCIINTGIAGSLDARINIGDIVISTDALQHDMDAR
                     AFGYEAGQIPRVDTLSFPADETLVTLAKECCSRVNPEIGTFTGRVVSGDQFISDKDKK
                     QWISETFSGMCTEMEGAAIAQVCYFNRIPFLILRAISDKADDSAGVDYAVFEAQAIRH
                     SVNLMTEMVRCM"
     assembly_gap    2169..2268
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2345..2593
                     /locus_tag="ACZPCG_02550"
     CDS             2345..2593
                     /locus_tag="ACZPCG_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002605485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HIR1283985.1"
                     /translation="MTYKTHGVCSRAITFDVDGNKLTNVQFAGGCSGNTQGVARLVEG
                     MDVDEAIRRLEGIQCGPRPTSCPDQLAQALKEYKAQLA"
     gene            2708..5692
                     /locus_tag="ACZPCG_02555"
     CDS             2708..5692
                     /locus_tag="ACZPCG_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HIR1283986.1"
                     /translation="MAKLPEKGQTLWGFTVTERNSISMLGAETAELFHPFSGARLLYI
                     HNDDRELGFNLIYRTPQLDNLDSNHILEHLMLCSCPSYPSRDVFFDMDSKSYSTFMNG
                     ITDNTCTCYPVCSLSMEQLIKLMDVFLCCMEEPEALKDHRFFMREGLRFELEDTDGEL
                     SMLGTVLSEDWGHLTDLEENADSAAAQALYEGQIAANLLGRAHFHYKEITFEKIRQTY
                     EQLYDYSNCLMILYGDADLETVLRFLDEKHLSRFKSRNLNLRPLFGGPVSSGFRKTEV
                     KSPAYEGSPAEHSSVIDYTIDLSGLSQEDVICMGFCAGMMDQDTSPVQRAARSLGLNN
                     IIDVYLDTALARPVLKFRLQNGDKEQQELFFQAVNQGLADMAENGIADELYHAVIKEN
                     RLADLLMRETPHLGFHLSEDIGRFWSLTGRTDYYSLYERTFTHFSADQEQSILRRLAK
                     QVLNPPSAVLSAAIPVPGLAEALEQERDRWLREKKASMSPKELQALIRETADFKKWSA
                     QDKSCLDFLIQPEQLPEPEEDPPFFSGLRHGIFCCGSPSPLAGIGSYQLFFDLSELSG
                     EDWNYLTLYQMLLSELDTRRFCVEQQKNMEQEYLYDCTFDELYPDADADENSRPMMSV
                     VWSGLTEDFEISLDFLLELMRNGNYEDRETISQVIEKYLPDYDLSKSENGPSLAYSLA
                     ERYIRQDSRFRFQLNSPEIYHFLKNILTILIREQAHPDDEAARTDANQISSSLYTLAQ
                     KLVSRRKLIFLAAAENASLEHILDRASTLLSQLPERNENGFMFSNHLSLPSWDQAPSL
                     RTAACIDSSLEELRMLGDFKKVPEFKGRFLPWLLAAGDKYVKPAVRYQGRAYDSGIDF
                     LLPAGYFTLWSTEDPDIESTLSVFNRTGQALAELKLTPEDLQGYILSAYAQALPPIGI
                     LNSRMRAMRRYIMGISTVYINEMIRNIRNSTVNDQKEASDLICHLLKSGPVSVVGNEK
                     IILEYKNRFDEILKIKEKNGTV"
     gene            6043..6363
                     /locus_tag="ACZPCG_02560"
     CDS             6043..6363
                     /locus_tag="ACZPCG_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR1283987.1"
                     /translation="MVFNTGSALLDAIVLAAVSREKEGTYGYRITQQVREAIDISEST
                     LYPVLRRLQKDDCLEVYDRAIDGRNRRYYRLTEQGRLQLQLYQREWAVYSQKISHIIK
                     EGDS"
     gene            6360..7160
                     /locus_tag="ACZPCG_02565"
     CDS             6360..7160
                     /locus_tag="ACZPCG_02565"
                     /inference="COORDINATES: protein motif:HMM:NF046861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HIR1283988.1"
                     /translation="MNREQFMKQLSYLLQDISDEDRTDALDYYENYLDEAGFGYETDI
                     TNELGSPERIAAIIRTSLLSNPDETGEFTDSGYENSRFKDPGYELAKQHRETDTFTGA
                     DRSKHPYTSCGSESGQTYGQAQQSRSSFHPGAERSGPRTSRLLKLLLWIVLLIVGAPL
                     LLGIGGSIAGLTAAIVGIAFALLISLGVLTGGAFITAILALPLGIVSLFIHPVQGIFT
                     AGTGLAALGAGFLLLALCLLFYGRLIPRLICRIIDWISGMLHKGRSQR"
     gene            7157..7318
                     /locus_tag="ACZPCG_02570"
     CDS             7157..7318
                     /locus_tag="ACZPCG_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1283989.1"
                     /translation="MNKLIRFCIIAGLIFLLSGLLLSACGAAMGGAAYPRFRPHRIHR
                     IMEHLDDCI"
     gene            7367..7564
                     /locus_tag="ACZPCG_02575"
     CDS             7367..7564
                     /locus_tag="ACZPCG_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HIR1283990.1"
                     /translation="MNKRLYRSVSNRMLSGVCGGIGEYFDIDPTLVRLAWAIFTCVTI
                     GAGVAAYVIAAIIIPADNNIR"
     gene            complement(7593..8432)
                     /locus_tag="ACZPCG_02580"
     CDS             complement(7593..8432)
                     /locus_tag="ACZPCG_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006862831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR1283991.1"
                     /translation="MIDQAGIMMAQGALIASERIIGVNDNMAQSHYHEYFELYYLETG
                     ERFHMVQDRCYKMTAGEFIIFPPYVMHYSYGEKDMPFKRLVLYFRQEELGSDEIFREL
                     CRERGICRLDTKKRTQIHRIMEQLLEEQKDPGRFTVSYRKALLNLLAAVIVRADLPPV
                     RQEKRSRINEIISYIHSHYYEDLKLECLAQQFYISPWYLCREFKRYTNSTLVNYINVT
                     RIMNAQRSFMETDKNVTQISQEVGFSSLTHFNRVFKAVTGTTPSEYKRQFRTYKAKTA
                     DSR"
     gene            8690..11194
                     /locus_tag="ACZPCG_02585"
     CDS             8690..11194
                     /locus_tag="ACZPCG_02585"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HIR1283992.1"
                     /translation="MKVCMNAGNVTKKGSVFCISTNCIEIRLLFLTDHILRIRAGFDG
                     DFCEESYSLVMTAWEDRMDGFMKHYRKRVAPAEAELIEDSAVQAVIQGKELKVVIEKD
                     PFRICVYDREGSQIHADIPDLAYLEDSNHRRIHTSEINAQDCFFGFGEKSGEFNKAQK
                     FMVMSPGDSMGYDPKETDSLYKHIPFYIKLNPGTKKAVGYFYHCTWECDFDMGREKRN
                     YWKPHSRFRTDGGDIDLFLIAGPSISQIVERYTDLTGKSALLPKYALGYLGSSMYYPE
                     LEENCDDAITEFIDTTKEEGIPVDGFQLSSGYCAIETEEGIKRCVFTWNKKRFKNPKD
                     FFAQMKKRGVCVSPNVKPGILLVHPRLEEMKAKGMFVKASDSDQPGIGTWWGGKGMFT
                     DFTSSSTRENWKMMLKENVLDYGTSSIWNDNCEYDSMVDKDCRVSFEGKGATIGQLKS
                     VMSNIMCHITDEAIHETFENTRPYIVCRSGHCGIQKYAQTWAGDNLTCWDSLKYNIAT
                     ILGMGLSGVANQGCDIGGFFGPAPEAELLVRWIQNGIFQPRFSIHSTNTDNTVTEPWM
                     YSGCTGYIRSAILLRYRLFPYLYSLMERAHTTGLPIMEPMCYAFQNDPACYEDGINFM
                     FGDSLLVANVVEKGAKIRSVYLPEGSAFYDFYTREVYEGGQTIEFPVNLGSIPLFVRS
                     GALIPMASNQMMNLMNETVTGLTILCAPDKDGSFILYEDDGVSMDYEKGLYLKTVITM
                     TAGERTVIGFKQEGSFETSVETIALDMIHREKAPYWVSVNGEILPHFLHRRKFEEAGC
                     GWYYSQTLKSVQIKYNNPKKDHQVLVSFEQFDLIGM"
     gene            11256..12278
                     /locus_tag="ACZPCG_02590"
     CDS             11256..12278
                     /locus_tag="ACZPCG_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR1283993.1"
                     /translation="MKKLAAISAALVIAAAGLSGCASQSAASSAEATRENPMILSLAH
                     GLSETHTVHIAMTEFAQNVEELTDGRIQVQIFPNGQLGSENENMEQLMAGVISMTKVS
                     APGLATYNEAYHTFGLPYIFDSTDDFYHKMDSRPMQDFFLSTENDGFVTLTYYTSGAR
                     SFYTKDKAIRTPEDLKGLKIRVQDMKSQTDMLKALGGIPVAMSYGDVYTSLQTGIIDG
                     TENNETALTTGKHGEICKVFSSDQHAMIPDVMVMSSKVWKSITPEDQQIILEAARKST
                     DSHKIAWNTAIEEAKKEASETMDVTFVEDVDKEAFRQATAGMISDYCSQYPGVQTLLD
                     IINSAE"
     gene            12289..12792
                     /locus_tag="ACZPCG_02595"
     CDS             12289..12792
                     /locus_tag="ACZPCG_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009268194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIR1283994.1"
                     /translation="MREFLTSVKKWMTFLLSRIATILLSVMTFLVLYQVFTRYVLNSP
                     AAFTEELVRYSLIWTGFIGAAYAFSTREHMSLVLLQESLKPEGRRILMICIDTLILIF
                     AIFIITIGGFKLAMSAQKVYSALLGIPRSLVYAMAPVSGIFIIIAQLINLYEDVTGIK
                     IEGGNES"
     gene            12789..14090
                     /locus_tag="ACZPCG_02600"
     CDS             12789..14090
                     /locus_tag="ACZPCG_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR1283995.1"
                     /translation="MSIGITTAVLFAVFAALLFLGCPISVGIVISSIVAAVSSLSWDQ
                     ITFITMQKMNSGVESFSLLAIPLFILAGNIMNNGGIAKRLVNFAQLFVGWCPGSLALA
                     NIVGNMLFGALSGSSVAAASAMGGCIYPIQKEEGYDPAFATAANIASAPTGLLIPPTS
                     AFIVYSTVAGGVSISTLFMAGYVPGLLMGLGSMVVAFLYAKKHKYPTSGFPSMQTAVK
                     VTLEALPSLLLIIIVIGGIVSGIFTATEGAGISVLYCLILSLCYKSINFKSFSKILVS
                     SACTSGIILFLISASSAMSFVMAYSGIPAAISTAIMSVSSNKYVILLLMNVILLVIGM
                     FMDITPAILIFTPIFLPIAESLGMSNIQFGVMLIFNMCMGNITPPVGSVLFVGCGISK
                     ISIEQVAKTLLPYFIVLFILLLAVTYIPVLSLGVPSLMGLL"
     gene            complement(14087..16336)
                     /locus_tag="ACZPCG_02605"
     CDS             complement(14087..16336)
                     /locus_tag="ACZPCG_02605"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HIR1283996.1"
                     /translation="MEMIMQIFKEDAELQALLRTVLEGICSDFTDEAYSLELLEEKFH
                     SFARPGMEEPELWAALVKAAVELTSAQSPKWEMIAARLLGASFRRELSGRLKVLGITD
                     FYSKLCYLTEEKLYGEYILEHYTKEEIQEAETFLCPERDHLFTYSGLDLLLKRYVIRS
                     RRQEPLETPQEMFLGIALHLAMEEKKDRLYWVRKFYDMLSRMYVTMATPTLSNARKPY
                     HQLSSCFIDTVSDSLEGIYRSIDNFARVSKFGGGMGLYFGKVRAAGSSIRGFEGAAGG
                     VIRWIRLVNDTAVAVDQLGMRQGAAAVYLDAWHRDLPEFLQLRTNNGDDRMKAHDIFP
                     AVCYPDRFWRLAKENLDAPWHLFCPHEIHEVKGYFLEDYFGEEWETRYEECVADSRLS
                     RRTVTVKELVRLIIKSAVETGTPFAFNRDIVNRANPNSHKGMIYCSNLCTEIAQNMSP
                     METVSQTVETEDGDPVVVTRVRPGDFVVCNLASLSLGNIPLEEDGVLEDTVWTAVRAL
                     DNVIGLNFYPLPYAELTNHAYRSIGLGVSGYHHMLAKRRIKWESREHLLFVDRLFERI
                     SQAAIEASADLGKEKGSYPLFEGSGWQTGTYFSRRGYTGSRWKQIAAKAAQGMRNAYL
                     LAIAPTSSTSILSGTTAGIDPIMHRFYLEEKKGAMLPRVAPELTPESYWYYKNAHLLD
                     QSWSIKACGIRQRHIDQAQSMNLYITNSYTFRKVLNLYIQAWEEGVKTIYYVRSKSLE
                     VEECESCSS"
     gene            complement(16355..17398)
                     /locus_tag="ACZPCG_02610"
     CDS             complement(16355..17398)
                     /locus_tag="ACZPCG_02610"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HIR1283997.1"
                     /translation="MDQLTKKPLFNPQGDTSLNARRMIGGNTTNLNDFNNMRYTWVKD
                     WYRQAMNNFWIPEEISLGQDKKDYPKLAPAERAAYDKILSFLVFLDSIQTANLPNIGE
                     YITANEINLCLNIQTFQECIHSQSYSYMLDTICSPIERDAILYQWKTDEHLLRRNTFI
                     GECYNGFQENKSPEAFLRVLMANYILEGIYFYSGFMFFYNLSRNGKMPGSAQEIRYIN
                     RDENTHLWLFRNMIRELKQERPELFTDKNVKMMKELLMEGVRQEILWGEYVIGDRISG
                     LTAEMIRDYIYYLGNLRWVSLGFDPVYEGFEKEPENMAWVSQYSNANLVKTDFFEARS
                     TAYAKSTALIDDL"
     gene            17560..18438
                     /locus_tag="ACZPCG_02615"
     CDS             17560..18438
                     /locus_tag="ACZPCG_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3881 family protein"
                     /protein_id="HIR1283998.1"
                     /translation="MHKYMRTIGFSQYTSRQDLDKLLKKLVKDASKSDILKESDGEMF
                     CELRAQTAPGMGVAIAGRLSQRGIFKQEYYFPYVESSQVTSTADCSIQRHTERETFAG
                     LLDEYRVGISLIFYMENSMEYRLRLKNSQPILPKSASLSALSIHGKILLPVQKTPQQT
                     EDRKKAASKRSRLIEAAKRGDEEAIETLTVEDIDLFSSVSRRVAREDIYSIVDTCFMP
                     SGIECDQYSVIGNILSVDRRINRITGEEIFLLGLECNDLLFTAAINAADLLGEPVPGR
                     RFKGQVWIQGNVCFEA"
     gene            complement(18441..19193)
                     /gene="yaaA"
                     /locus_tag="ACZPCG_02620"
     CDS             complement(18441..19193)
                     /gene="yaaA"
                     /locus_tag="ACZPCG_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005351477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HIR1283999.1"
                     /translation="MRIILSPAKKMKEDPDTLAYMGLPEFLDQTEEILAWLRSRTQEE
                     LKRLWVCNDRIVGQNVERLAHMDLRKNLTPAVLSYEVIAYQYMAPAVFETGQFEYIQE
                     HLRILSAFYGVLRPMDGVTPYRLEMQARAAVGGYKDLYALWGNRLYEAVRDESGIILN
                     LASKEYSKCIEVCLSEEDRYITPVFCEEAGGKLVTKGTYAKMARGEMVRFMAENRIED
                     PSEIRSFDRLGYRFREELSSDTRYIFERVKAE"
     gene            complement(19190..19645)
                     /gene="bcp"
                     /locus_tag="ACZPCG_02625"
     CDS             complement(19190..19645)
                     /gene="bcp"
                     /locus_tag="ACZPCG_02625"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HIR1284000.1"
                     /translation="MLNTGIKAPNFELPDQNGTIHTLEEYRGKKVILYFYPKDNTPGC
                     TKQACGFSERYPQFMEKGTVVLGVSRDTVASHKKFEEKYGLVFTLLSDPERKVIEAYD
                     VWKEKKNYGKVSMGVVRTTYLIDENGIIIRANDKVKAAEDAEKMLGELE"
     gene            complement(20290..20745)
                     /locus_tag="ACZPCG_02630"
     CDS             complement(20290..20745)
                     /locus_tag="ACZPCG_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007157470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HIR1284001.1"
                     /translation="MADIHYISIDEARDIHLATIRNSGGGDCGELDIGKLESVLCHIQ
                     NDNYYPTFLDKLLHLFYCSCKFHCFCDGNKWIAITLTAMSLLKNGYMAVANIYFREME
                     NISYHVAAGHIDEDLLRRILAAILTCTYDSDEELKLDIFNAISVEDVFY"
     gene            complement(21468..21842)
                     /locus_tag="ACZPCG_02635"
                     /pseudo
     CDS             complement(21468..21842)
                     /locus_tag="ACZPCG_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015572846.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(21928..22221)
                     /locus_tag="ACZPCG_02640"
     CDS             complement(21928..22221)
                     /locus_tag="ACZPCG_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284002.1"
                     /translation="MWLIKLPFRIIALPIMAIVAVFSIFYSLALNLSAMVVGLLYLVL
                     VVCIVMLLFQHVWIYAALVFGFAVLVFRAVMFAEMISLGLEALTGKLLQFIFS"
     gene            complement(22330..22506)
                     /gene="mobC"
                     /locus_tag="ACZPCG_02645"
     CDS             complement(22330..22506)
                     /gene="mobC"
                     /locus_tag="ACZPCG_02645"
                     /inference="COORDINATES: protein motif:HMM:NF017524.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HIR1284003.1"
                     /translation="MRSREGLLRYCSNNLNQYAKRANETGSIYTEDIKDLQNRQNEIR
                     EITKEILARLATIQ"
     gene            complement(22609..23655)
                     /locus_tag="ACZPCG_02650"
     CDS             complement(22609..23655)
                     /locus_tag="ACZPCG_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004082173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HIR1284004.1"
                     /translation="MRDFNYSTIREQKWDSEILGLIAAIYKEAGKQELYLKQRPEELE
                     KLVEIAKIQSTEASNAIEGIVTTNTRIRQLVEEKTTPRNKDEQEIAGYRDVLSIIHES
                     FDAIPLTRNYILQLHKIMYSHMNNPMAGQTKNVQNYISATYPDGHIEILFTPLAPFET
                     PKALDRICEEYNRVIGNMELEPLIAVPVFIHDFLCIHPFNDGNGRMSRLLTTLLLYRS
                     GFYVSKYISLEAKIAKNKDLYYEALSRSQHGWHEGTEDAVPFIKYLLGTILAAYKDFG
                     DRFAIVEEKLPAIDMVRKATLNKIGRFTKQDIREQCPSLSLSSVEGALRKLVAAGELK
                     REGTGKSTCYIRLK"
     gene            complement(23934..24017)
                     /locus_tag="ACZPCG_02655"
                     /pseudo
     CDS             complement(23934..24017)
                     /locus_tag="ACZPCG_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934794.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family mobile element protein"
     gene            24156..24641
                     /locus_tag="ACZPCG_02660"
                     /pseudo
     CDS             24156..24641
                     /locus_tag="ACZPCG_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002578421.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(24991..26325)
                     /locus_tag="ACZPCG_02665"
     CDS             complement(24991..26325)
                     /locus_tag="ACZPCG_02665"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002573882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR1284005.1"
                     /translation="MENYDILIKNCQVLNPDMNVSSTCSVGIKDTWIKKIGSADEFVD
                     SVATETLDGKGKLVMPGLIDGHTHTCQQLLRGRVADEYPMVWTRFLVPFESNLLPEDS
                     YVSGQLACLEMIKNGTTAFADSGGVHMERVADAVIESGMRAAIAKSTMDMGNAITGAM
                     KETASEAIDHTKELYKNYQGAGDGRVDIWFAIRQVMTCSRDLITMVGECAKELHTGIH
                     AHLCEHKDEVSFCLQNYQKRPAEFLDEMGILGPNLLTAHNVMLSDHDIALMAERGVKM
                     IHCPRANLANHGFPKAPQILEAGASVGLGCDGAAPSNLDIFDEMKVLRYSMLAYWGLP
                     SFNPVVMTCPTLLKMASQGGVNAIGHGDILGSVKEGKKADLILLNIDQPHITPTQNLV
                     NTIVDAANGHDVIDSIINGKLVMKNREVLTLDEERIRFEATQHMNEIIKRAY"
     gene            complement(26355..26816)
                     /locus_tag="ACZPCG_02670"
     CDS             complement(26355..26816)
                     /locus_tag="ACZPCG_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002573883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284006.1"
                     /translation="MVRKYFVQQLGLLLMVSVIIILTNVIGYKMPVKDSIIGVLLLSA
                     ISLIGLTISKFMSRFVKLPSMMYVSLLGLLLACPVSPVKDIVVETTTPVAFLAPATAL
                     GAFAGISLGKDLKDFTKMGWKLVLITLFVITGTFIFSALVADIVLKMTGAI"
     gene            complement(26817..27632)
                     /locus_tag="ACZPCG_02675"
     CDS             complement(26817..27632)
                     /locus_tag="ACZPCG_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002573884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HIR1284007.1"
                     /translation="MEKLKIVIKKYWKIYLAALVISAVCDLIGTVKLNIGVGTLTLFP
                     MVFAVIFGGLLGPDIFKVFKTEECKIGGSLVLVVLAPFMAKMGISSGANVAKLVSVGP
                     ALLLQEFGNLGTILLSMPLALLLGLKKESIGACYSINRDSNLGLTTDIFGPDAPETKG
                     TFAVYIVGSVIGTVFISILASVVASWNIFDPIALGMASGVGSGSLMTAAAGTLGEIYP
                     QQAENIMIMGGASDMLTGITGIYMGTFVGLPLAKKLYAILEPKIGRKNNQEVK"
     gene            complement(27904..>28142)
                     /locus_tag="ACZPCG_02680"
     CDS             complement(27904..>28142)
                     /locus_tag="ACZPCG_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284008.1"
                     /translation="RTESQKDIQLALENVFVDLKKKTFQLGDLQLQTKDIDIPLDNGF
                     YRLKIGDLDLRKSGLRLDHVHLVSPYSKMEFALR"
BASE COUNT         7552 a         6821 c         5888 g         7781 t
ORIGIN      
        1 acagctttgt tattactgat ataccaatac tttctgcgac tgtttgctat tgttgccgca
       61 tagtgttctg gaattcccag cttgactaga ttcttatatt ttgttctagg tttcttccac
      121 tgtttccata tacacatacg tattctgtga tagagccatc cgttaatgtc agagatttgc
      181 ccgtgagtta gtatgttcct cacatccagc tcccttcaga tcccacctca cgatggacac
      241 ccttgccttc ggctatatcc ttcccactac cgggcggatt cgggacttta acccgttaga
      301 aacgtgcgcc gctaggcgca ccatacaaaa agctgccccc agtccccaag ggaaccggga
      361 gcagcacatt tctccaaatc tcataactat gatcaggata taaatccttt aagcatctca
      421 cccagcatcc gcttctgatc cgggttcagg tcctgaagcc agttttcacc ggaacggtcc
      481 tctctggcgt ctctggatac ttccctgaga gtttccctgc tggctgcggt ctgttctgca
      541 ctgcgtctgg catgaactct gcggtctctt ccgatggagc gcacaggagc cgcttcccgg
      601 cgttcttcct ctgccgccgg agcaggctgc tggtctgatg ccgtctcacg ctccatccgg
      661 gaccacatta cattttccac atgatcctga agacaggtaa ggagatgctg gcgctttgcc
      721 tccagattta caccgtaatc cacaaacacg gtggccatcc ctccaaagat acccataacg
      781 ccatacagga caatgtagta attatcgctt ctgttccaat aggagaggaa cgccgccgcc
      841 cctcctgcga gaaagcataa aagcgtcagc tgtcctccca gattttcaaa tccctgaagg
      901 ctgatcccca taaaacgcag gctgtaaatc tggcgttcca gatatactct ggtgttgttc
      961 attcccatat tgatcctgta tgtatcctcc gcattctggc gcagggcgcg cagataccgg
     1021 ttttttgcaa gcgccagatt ggagctctcc cgaatgagac gtttatagaa gcttcccgct
     1081 gcgagcctta ccataattcc tcccagacaa attgctgcca gcagatacag agctcttcca
     1141 gtctccaaaa aactcatcat aaccaattcc cctttctata cactaccatc atttctctgc
     1201 agcctatagg ccgctgcagg ccgccgaacc cgtccctgca ggtctggaac attttccgaa
     1261 aatgatccga gcggcttcgg taaggtctat tatagtccgg gaattggatt tgggaagttg
     1321 agaaccccta gaatcgttcg tcaaattccc ccacgaaacg attcttaagg taaaatgctg
     1381 ctacatgcag cgaaccattt ccgtcatcag gtttacgctg tggcggatgg cctgagcctc
     1441 gaaaacagcg taatctacac cggcgctgtc atctgccttg tcagaaatag cgcgcaggat
     1501 caggaatggg atacggttaa aatagcacac ctgagcaatg gccgctccct ccatctctgt
     1561 acacataccg gaaaatgttt cactgatcca ctgcttttta tccttatcag aaataaactg
     1621 atcccccgaa acaacacggc ctgtaaaagt gccgatctcc ggattcaccc ggctgcagca
     1681 ttcctttgca agagtaacca gtgtttcatc tgccggaaat gaaagcgtat ctactcttgg
     1741 gatctgcccc gcttcatagc caaaggctct ggcatccata tcgtgctgca gagcgtctgt
     1801 agaaatcaca atatcaccga tattgatccg ggcgtccaga gaacctgcaa ttcccgtatt
     1861 gatgatgcag gaaggcgcat agcggtccgc cagaatctga gcgcacatgg ccgcatttac
     1921 cttgccgatg ccggaacgga ctaccaccgt gtcctttcct tccagctttc ccttataaaa
     1981 ttccatgcag gcccgggact ccacctgtac ctctgtcatt ttttccttta acatagccac
     2041 ttcttcatcc atggctccga taattgctat cattgcaatt cctccttatt ctgatcggcg
     2101 gcgctgtctg aaagccgctt tttctcaatt atactgtggc ctgcaccatt ttttcaagca
     2161 tcttcttgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa tccacccgcc
     2281 ttggggcata atcgaatctg cattgactat gaaacaacga ttttaattgg aggttaatta
     2341 catgatgaca tataaaacac acggcgtatg ttccagagcc attacctttg atgtagacgg
     2401 caataagctc accaatgtcc agtttgcagg cggctgctcc ggcaataccc agggcgttgc
     2461 aagactggta gagggtatgg atgtagatga ggcgatccgc cgtctggaag gcattcagtg
     2521 cggcccccgt cctacctcat gtcctgacca gctggcacag gcattaaagg aatataaggc
     2581 acagctggca taaaacagca ctgcttcgga ttcttataaa aactgtatcc atatgctgta
     2641 ctgcggatcg tctgccgccg gtacagcatt ttttacgcac tgcattttat aaaaaaggag
     2701 atttgccatg gcaaagctgc ccgaaaaagg ccagactctg tggggcttta ctgtcacaga
     2761 gcgcaactca atttccatgc tgggagctga aactgcggaa ctgttccacc ctttcagcgg
     2821 agccagactt ttatatatac ataacgacga ccgggagctt gggtttaatc tgatctaccg
     2881 gactccgcag ctggataatc tggattccaa ccatatatta gagcatttga tgctctgttc
     2941 ctgcccttcc tatcccagcc gggatgtgtt ctttgacatg gacagcaaaa gctattccac
     3001 ctttatgaac gggatcacgg acaatacctg cacctgctat ccggtctgct ccctgagcat
     3061 ggagcagctt ataaagctta tggatgtctt tttatgctgt atggaagaac cggaggcttt
     3121 aaaggatcat cggttcttta tgagggaggg gcttcggttt gaactggagg atacggatgg
     3181 agagctttcc atgctgggaa cggttctcag cgaagactgg gggcatttaa cagatctgga
     3241 ggaaaatgcg gacagcgcgg ctgcccaggc attgtatgaa gggcagattg cagccaatct
     3301 tcttggaaga gcccatttcc attataaaga aatcaccttt gaaaaaatcc gtcaaaccta
     3361 cgaacagctt tatgactatt ccaactgcct gatgatcctc tacggagacg ccgacctgga
     3421 gacggttctg cgttttctgg atgaaaaaca cttatcacgc ttcaaaagca gaaacctgaa
     3481 tcttcgcccc ctttttggag ggcctgtgtc ttccggtttc cgaaaaacag aggtcaaaag
     3541 tccggcctat gaaggaagcc cggctgaaca ctcctctgtg atcgactata ctattgatct
     3601 aagcgggcta tcccaggagg atgtgatctg tatggggttc tgcgccggaa tgatggatca
     3661 ggatacctct ccggttcaac gggccgccag atcccttggc cttaacaata ttatcgacgt
     3721 ctatctggat acggcgctgg cccggccggt tttaaaattc cgccttcaaa atggggataa
     3781 agaacagcag gagctgtttt ttcaggccgt aaatcagggg cttgcagaca tggcggaaaa
     3841 tggaattgca gacgagctgt accatgcagt cataaaggaa aacaggctgg ccgatctcct
     3901 tatgagggaa acgccccatc tgggctttca tctgtccgag gatatcggac gcttctggag
     3961 ccttaccgga cgaaccgact actactccct ttacgaacgt accttcacac atttcagtgc
     4021 agatcaggaa cagtccattc tgcgcaggct ggcaaagcaa gtattaaacc cgccgtcagc
     4081 cgtactgtct gcagccattc ccgtacccgg tctggcagaa gctctggaac aggaacgcga
     4141 tcggtggctg agagaaaaaa aggcttccat gtccccaaaa gagcttcagg ctctcatccg
     4201 tgaaacagcg gattttaaaa aatggagcgc ccaggataaa agctgtcttg attttctgat
     4261 tcagccggag cagcttcccg aacccgaaga ggatccgccc tttttctccg gcctgcgcca
     4321 tgggatcttc tgctgcggtt ccccttctcc tcttgcagga atcggcagct accagctctt
     4381 ttttgatctg agcgaactaa gcggggaaga ctggaactat cttacattgt atcagatgct
     4441 gctctcagaa ctggatacac gccgtttctg tgtggaacag cagaaaaaca tggaacagga
     4501 atatctttat gactgtacct ttgatgaact gtatccggat gcagatgccg acgaaaacag
     4561 ccggcccatg atgagcgtgg tgtggtcggg acttacggag gactttgaaa tcagtctgga
     4621 ttttcttctg gagctgatga gaaatggaaa ttacgaagat agggaaacga tttcacaggt
     4681 gattgaaaaa tatctcccgg actacgatct gtccaaaagt gaaaacggtc cttctctagc
     4741 ctacagtctg gcagaacggt atatacggca ggacagccgt ttccgtttcc agctcaattc
     4801 acctgagata taccactttt taaagaatat tttaaccatc ctgatcaggg agcaggccca
     4861 tcccgatgat gaagcggcca ggacagacgc aaaccagata agcagctcat tatataccct
     4921 ggcccagaag ctggtaagcc gcagaaagct gatctttctg gcagctgccg aaaacgcttc
     4981 actggaacac atcctggatc gtgcctccac gttgttgagc cagcttcccg agcgtaatga
     5041 aaacggtttc atgttttcca atcatctttc acttccatca tgggatcagg ccccgtctct
     5101 tcggacagcc gcctgcattg acagttctct tgaagaactt aggatgctgg gagattttaa
     5161 aaaggttcct gaatttaagg gccgtttcct tccatggctt ctggcagcag gcgataaata
     5221 tgtaaagcct gccgtgcgct atcagggacg agcctatgac agcggaattg attttcttct
     5281 tccggccgga tattttaccc tatggagcac agaagaccct gatatcgaat caacactttc
     5341 cgtatttaac cgtaccggcc aggctctggc agaactaaag ctgacgccag aggatcttca
     5401 gggttacatt ttaagcgcct atgctcaggc tctccctccc atcggaatcc tgaacagccg
     5461 tatgcgggct atgcgaagat atattatggg gatctctact gtatacatta atgaaatgat
     5521 acgcaatata cgtaacagta ccgtaaatga ccaaaaagaa gcgtcagatt taatctgcca
     5581 tcttttaaaa tccggtccgg tatctgtagt cggaaatgaa aaaataatcc tggaatataa
     5641 aaaccgattt gatgaaatac tgaagataaa agaaaaaaac ggtacggtat gaaactgtgg
     5701 ggagagaaat ctccccataa attggtttga gccggcaagg ctgtccatat gccaaccccg
     5761 ccggcttagt gatctatgct gcagcgataa atgctgctgt cttctatgta aaaagctctg
     5821 actgcttgtt tatgtccttt agtataacct ttaattgtgt acgaagtttg accacatggt
     5881 aaacagtttc taaaatagtt taagaaaaaa taacaaacat ttcctttttc tttggatttt
     5941 cttttattga catataatat tatacatcat ataatattga tgtaatcatt cacagcggca
     6001 gatcatacat tttcatgccg ccaacacacg gaggggatgc ttatggtatt caacaccgga
     6061 tctgcccttc tggatgccat tgtactggct gccgtttcac gggaaaagga aggaacctat
     6121 ggctaccgga tcacccaaca ggtccgcgag gccattgaca tatcagaatc taccttgtat
     6181 ccggtgcttc ggcgtctgca gaaggatgac tgcctggagg tctacgaccg ggcgattgac
     6241 ggcagaaacc gccggtatta ccggctcaca gaacagggac ggctgcagct gcagctctac
     6301 caaagagaat gggcagtcta ctcccagaag atttcccaca tcataaagga gggggactct
     6361 tgaatcgaga acagtttatg aaacagcttt cctatctgct tcaggatata agcgatgaag
     6421 atcgaacaga cgctctggat tactatgaaa attatcttga tgaggccgga tttggatatg
     6481 aaaccgatat cacaaatgag cttggaagcc cggaacggat cgcagcgatt atccggacca
     6541 gtcttttaag taatccggat gagacaggag aatttacaga cagcggctat gaaaattccc
     6601 gctttaagga tccgggctat gagcttgcaa aacagcaccg tgaaaccgat accttcaccg
     6661 gagctgaccg cagcaaacat ccgtatactt cctgcggatc cgaatcaggc caaacgtatg
     6721 gacaggcaca gcaaagccgc agttcctttc atccgggagc agagcgttcc ggccccagga
     6781 ccagccgcct gttaaagctt cttttgtgga tcgtccttct tatcgtagga gcgcctcttc
     6841 ttctgggaat cggaggcagt atcgcaggat taaccgcagc catagttggc attgcttttg
     6901 ctctccttat cagtcttggt gttttaaccg gaggcgcttt tattacagca attcttgccc
     6961 ttcctctggg aattgtatcg ctttttatcc acccggttca gggaattttt accgccggaa
     7021 cagggctggc agccctggga gcaggctttc tcctcttagc cctctgcctt ctgttttacg
     7081 gccgactgat tccccgcctt atatgccgga tcattgactg gatcagcgga atgcttcata
     7141 aagggaggtc acagagatga ataaactgat acgtttctgc atcattgcag gcctgatttt
     7201 tcttctcagc ggactgctct tatccgcctg cggagcagcc atgggaggcg ccgcctaccc
     7261 cagattccgt ccccaccgga ttcaccggat tatggagcat ctggatgact gcatctgagc
     7321 ccaaaacttt atttaacatt tcaatacaaa ggaggtcttt ctgattatga ataaacgatt
     7381 ataccgttct gtttccaacc gtatgctcag cggcgtatgc ggcgggatcg gagaatattt
     7441 tgacattgat cccaccctcg tgcgcctggc ctgggctata ttcacctgcg ttaccatagg
     7501 cgcaggagtt gctgcctatg tgattgccgc aattatcata ccggctgata ataatatccg
     7561 ctaggcgaaa cagccgattc cgggctcata tgctaccggg aatcggctgt tttagcttta
     7621 taggtacgaa actgtctttt gtactcagaa ggagtggttc ctgttacagc tttaaatacc
     7681 cggttaaagt gagtcagact tgaaaatcct acttcctggc taatctgagt cacattttta
     7741 tccgtttcca taaaactcct ctgagcattc ataatacggg ttacattaat ataattaacc
     7801 agtgtgctgt ttgtatagcg cttgaactcc ctgcaaagat accaggggct gatataaaac
     7861 tgctgcgcaa ggcactccag ctttaaatct tcataataat gactgtgaat ataacttatt
     7921 atttcattaa tccggcttcg tttctcctgt ctgaccggag gcagatctgc cctgacaatt
     7981 acagctgcaa gcaaatttaa aagagctttg cgataactta ccgtaaaacg tcccggatcc
     8041 ttctgctcct ccaacagctg ctccataatc cggtgaattt gtgtacgctt ctttgtatcc
     8101 agacggcaaa ttccccgttc tctgcaaagc tctctgaata tctcatctga tcccagttcc
     8161 tcctgtctga aatacaacac cagccttttg aatggcatat ctttttcgcc atatgaatag
     8221 tgcattacat agggtgggaa aataataaat tcaccggctg tcattttata gcaccggtcc
     8281 tgtaccatat gaaaccgttc tcctgtttca agataataca gctcaaaata ttcatgataa
     8341 tgggactgtg ccatattatc attgactcct atgatacgtt cactggcaat taaagccccc
     8401 tgagccatca tgatgcctgc ctgatcgatc atttttacta ctccctcctc ctttgcattt
     8461 atttaagtta tgtcttttgt tgtaagtgct tacattttca ccataatttt tattgacttt
     8521 ctatatcctt ttctcttttt ttatttcatc cacacaaaaa aacgcaagaa atgtaaaatt
     8581 tattacaata tactgcaata ttcgtctggt tttgccgatt ctgttttatt atcatattac
     8641 cacagaaccg atcgaaagta tacattttac aaacaggagg aactcattga tgaaggtatg
     8701 tatgaatgcc ggcaatgtaa caaagaaggg ctctgttttc tgcatttcca ccaactgcat
     8761 agaaatccgc cttctctttc ttacagatca tattctgaga atcagagcag gatttgatgg
     8821 cgatttttgc gaagaatcct acagtctggt aatgactgcc tgggaagaca gaatggacgg
     8881 ctttatgaag cattacagaa agcgtgtagc accggcagaa gctgaactga ttgaagacag
     8941 cgcagtgcag gctgtaattc agggtaaaga actcaaggtt gtgattgaaa aggatccttt
     9001 ccgaatctgt gtatatgacc gtgaaggctc acagattcat gcggatatcc cggatcttgc
     9061 ctatctggag gattcaaacc accgccggat ccatacaagt gagatcaatg ctcaggactg
     9121 cttttttggt ttcggcgaaa aaagcggcga atttaataag gctcagaaat ttatggtaat
     9181 gagccccgga gactccatgg gatatgaccc caaagaaacc gattccctct ataagcacat
     9241 tccattttat atcaaattaa accctggcac aaaaaaggca gtcggttact tctatcactg
     9301 tacctgggaa tgcgactttg atatgggacg tgaaaaacga aactactgga agccccacag
     9361 ccgtttccgt acagatggag gcgatattga cctgttcctc atagccggac catctatcag
     9421 ccagattgtc gagcgctaca cagatctgac cggaaagtct gccctgcttc ccaaatatgc
     9481 cctaggatat ctgggttctt ccatgtacta tcccgaactg gaagaaaact gtgacgatgc
     9541 catcacggaa tttattgaca ccacaaaaga agaagggatc cctgtagacg gctttcagct
     9601 ttcttccggt tactgcgcca tcgaaacaga ggaaggaatc aagcgctgtg tgtttacctg
     9661 gaacaaaaaa cgctttaaaa atcctaaaga tttctttgca cagatgaaaa aacgaggcgt
     9721 ctgcgtatct cccaatgtaa agccgggtat tttgctggta catcccagac tggaagaaat
     9781 gaaagccaaa ggcatgtttg taaaagccag cgacagtgat cagccgggta tcggaacctg
     9841 gtggggcgga aaaggtatgt ttacagactt taccagctcc tctacccgtg aaaactggaa
     9901 aatgatgtta aaagaaaatg tgctagacta tggtacaagc tccatctgga atgataactg
     9961 cgaatatgat tccatggtag ataaggactg ccgggtatcc tttgagggaa aaggcgctac
    10021 catcggtcag ttaaaatctg ttatgtccaa tattatgtgc catatcacag atgaagcaat
    10081 ccatgaaacc tttgaaaaca cacggcccta tatcgtatgc cgttccggcc attgcggaat
    10141 tcagaaatac gcccagacct gggcaggcga taacctgacc tgctgggatt ccctgaaata
    10201 caatattgca accatacttg gaatgggact ctccggtgta gccaatcagg gatgcgatat
    10261 cggaggcttc tttggccctg ctccggaagc cgagcttcta gtccgctgga tccagaatgg
    10321 tattttccag ccccgtttct ccatccattc taccaatacg gataacaccg ttaccgaacc
    10381 ctggatgtac agcggctgta ccggctacat ccgcagcgca attctgctac gttaccgcct
    10441 gttcccctat ctgtattctc tgatggagcg ggcccatacc acaggacttc cgattatgga
    10501 acccatgtgc tacgccttcc agaatgatcc tgcctgctat gaggacggaa ttaactttat
    10561 gttcggtgat tctcttctgg tagcaaatgt agtggaaaag ggcgcaaaaa tccgcagcgt
    10621 ctatctcccc gaaggaagcg ccttctatga cttctataca agagaagtat atgagggcgg
    10681 acagaccatc gaattccctg taaatctggg aagcattcct ctgtttgtgc gaagcggcgc
    10741 cttgatcccc atggcatcaa accagatgat gaatctgatg aatgaaacag tcacaggact
    10801 taccatcctg tgtgcgcctg acaaagacgg ttcctttata ctttatgaag atgacggcgt
    10861 atccatggat tatgagaaag ggctatactt aaaaaccgtt atcaccatga ctgcaggcga
    10921 acgtactgtc attggcttta aacaggaagg aagctttgaa acctccgttg agaccatcgc
    10981 tctggatatg atccaccgtg agaaagcccc ttactgggta agcgttaatg gagaaatact
    11041 cccacatttc cttcaccgtc gtaaatttga ggaggctggc tgcggctggt attacagcca
    11101 gactctgaaa tccgttcaga ttaaatataa taaccccaaa aaggatcatc aggttcttgt
    11161 ttcctttgag caattcgatc tgatcggtat gtaagcttac aattgcagcc aaaaactgca
    11221 gatcactatt tttaagtatg aaaggagtaa ctagtgtgaa gaaacttgca gcaatcagcg
    11281 ccgctctggt tattgcagca gccgggcttt ctggctgtgc ttcccagagc gctgcttcca
    11341 gcgcagaggc taccagagaa aatcctatga tcctttctct ggctcatggg ctcagcgaaa
    11401 ctcataccgt acatattgcc atgacggaat ttgcacaaaa tgtagaagaa ctcacagatg
    11461 gaagaattca ggttcagatt tttccaaacg gccagcttgg ttctgagaac gaaaatatgg
    11521 agcagctgat ggccggcgtt atttctatga caaaagtctc agcccccgga ctggcaacct
    11581 acaatgaagc gtatcatacc tttgggctgc cgtatatctt tgacagtacc gatgattttt
    11641 accacaaaat ggattcccgt cccatgcagg actttttcct ttccacagag aatgacggct
    11701 ttgtaacctt aacctactac acctccggcg cacgttcttt ctatacaaag gataaggcaa
    11761 tccgcactcc tgaagattta aaaggcctga aaatccgtgt acaggatatg aaatcccaaa
    11821 ccgatatgct aaaagcctta ggtggtattc ctgtggcgat gtcctatgga gatgtgtaca
    11881 cctctcttca aaccggtatt attgacggaa cagaaaataa cgaaacagcc ctcaccaccg
    11941 gaaagcacgg agaaatctgt aaagtatttt cctccgacca gcatgccatg attccggatg
    12001 ttatggttat gagctccaaa gtatggaagt ccattactcc cgaggatcag cagattattc
    12061 tggaggcagc cagaaaatcc acagacagcc ataaaattgc atggaacaca gcgattgaag
    12121 aagcaaaaaa agaggcgtcc gaaaccatgg atgtcacctt tgttgaggat gtggataaag
    12181 aggcgtttcg tcaggctact gcaggtatga tttccgatta ctgcagtcag tatcccggtg
    12241 tacagaccct gcttgacatt atcaattcag cagaatagga gaggctatat gagagaattt
    12301 ttaacatctg taaaaaaatg gatgactttt cttttgtcca gaattgcaac aattcttctt
    12361 tccgtaatga cgtttttagt actgtatcag gtatttacac gttatgtctt aaacagccct
    12421 gccgcattta cggaggagct ggtacgttat tccctgatct ggaccggatt tatcggcgct
    12481 gcctatgcat tcagtacaag ggagcatatg tcgcttgtac tccttcagga aagcttaaag
    12541 ccagaaggcc gccggatcct tatgatctgc attgataccc tgattcttat atttgccatt
    12601 tttattatca ccatcggcgg ttttaagctg gccatgagcg cccagaaggt ttattctgcc
    12661 cttcttggca ttccaagaag tctggtttat gcaatggctc ctgtttcagg tatttttatc
    12721 attattgcac agctcatcaa tctttatgag gatgtgacag gtattaaaat tgaaggagga
    12781 aatgagtcat gagtattggt attacaacgg ccgtcctgtt tgcggtattt gcagcccttc
    12841 ttttccttgg ctgtcccatt tccgtaggca ttgtaatttc ttctattgtt gctgcggtat
    12901 cctccctctc ctgggatcag attaccttta tcaccatgca aaaaatgaac agcggtgtgg
    12961 aaagcttttc ccttcttgca atcccgctgt ttatcctggc aggaaacata atgaacaacg
    13021 gtggtattgc caaacgcctt gtaaacttcg ctcagctttt tgtaggctgg tgtcccggct
    13081 ctctggcttt agctaatatt gtaggcaata tgctctttgg agcgctttcc ggttcctctg
    13141 ttgccgcagc ttctgccatg ggaggctgca tctacccgat ccagaaggaa gagggctatg
    13201 atccagcctt tgcaacggca gctaatattg cttccgcacc taccggtctt ctgattccgc
    13261 caaccagcgc atttatcgta tattccacgg ttgcaggcgg agtatctatt tccacactgt
    13321 ttatggccgg ttatgttcca ggacttctca tggggcttgg ttctatggtt gtagcttttt
    13381 tgtatgcaaa aaaacataag tatccaacct caggctttcc atccatgcag actgcagtaa
    13441 aggttactct ggaggctctt cccagccttc tgctgattat catcgtaatt ggcggtattg
    13501 taagtggtat ctttacagca actgaggggg caggtatttc cgtactgtac tgcctgattc
    13561 tttctctctg ctacaaaagc attaacttta agagtttttc taaaatcctg gtaagcagtg
    13621 cctgcaccag cggcattatc ctgttcctga tctccgcctc ctcagctatg tcctttgtaa
    13681 tggcatattc aggaatcccg gcagccatca gtacggctat tatgtctgtt tcttctaaca
    13741 aatatgtgat cctgctgctg atgaatgtaa ttctgctggt aattggtatg tttatggata
    13801 ttactcctgc aatcctgatc ttcacaccga ttttccttcc tattgcagaa agccttggaa
    13861 tgagcaatat ccagttcggt gttatgctga tttttaatat gtgtatggga aatataacgc
    13921 cgcctgtagg atctgtcctt ttcgtaggct gcggtatcag caaaatcagc attgaacagg
    13981 tagcaaaaac tttactacct tactttattg tactttttat ccttctgctg gcagttacct
    14041 atatccctgt actttctctg ggcgtaccct ctctgatggg gctgctctaa gaagagcagc
    14101 tctcacattc ctccacctct aaggacttgg agcggacata gtaaatcgtc tttacgcctt
    14161 cttcccaggc ctggatatac aggtttaaaa ccttgcggaa ggtatagctg ttggtgatat
    14221 acaggttcat gctctgagcc tggtcgatat ggcgctggcg gatgccgcag gcttttatgc
    14281 tccagctctg atctaacaga tgggcatttt tataatacca gtatgattcg ggagtcagct
    14341 ccggggccac ccttgggagc atggctccct ttttttcttc cagatagaag cggtgcataa
    14401 tggggtcaat accggctgtg gttcccgaaa ggatactggt tgagctggta ggagcaatgg
    14461 ctaaaagata tgcattccgc attccctgag ctgcctttgc ggcaatctgt ttccatcggc
    14521 ttcccgtata gccccgtctg gaaaaatagg ttccggtctg ccagccgctg ccctcaaaca
    14581 gaggatagct tcccttttcc tttcccagat ccgcactggc ctcgatggca gcctggctga
    14641 tccgttcaaa aagcctgtcc acaaacagaa ggtgctcccg gctctcccat ttaatcctcc
    14701 gctttgccag catatggtga tagccggaaa ctcccagccc gatgcttctg tatgcatggt
    14761 ttgtaagctc tgcataggga agaggataaa aattaaggcc aattacattg tccaatgccc
    14821 ggactgcggt ccacacggta tcctctaaca cgccatcctc ctccaaagga atatttccca
    14881 gagaaaggct cgccagatta cacaccacaa aatcccccgg acgcaccctg gtaacaacca
    14941 caggatcgcc gtcctcagtc tctaccgtct ggctgacggt ttccatggga gacatattct
    15001 gcgcaatttc cgtacagaga ttggagcagt aaatcattcc tttatggctg ttgggattgg
    15061 cccggtttac aatgtcccgg ttaaaggcaa agggcgttcc cgtttccacc gcagatttga
    15121 taatcagacg caccagctcc tttaccgtca ccgttctgcg gcttaaccgg gaatctgcca
    15181 cacattcctc atagcgcgtt tcccattctt ccccaaaata atcttccaga aaatagccct
    15241 ttacctcatg gatctcatgg gggcagaaca gatgccaggg agcatccaga ttttcctttg
    15301 ccagtctcca aaaccgatcc ggataacata cagccggaaa aatatcatgc gccttcatcc
    15361 gatcgtcgcc gttattggtg cggagctgaa gaaattccgg cagatcccta tgccatgcat
    15421 ccagatatac ggcggcggct ccctggcgca tccccagctg atctacggct actgctgtat
    15481 catttacaag acggatccag cggatcacac ctccggccgc accttcaaag ccccggatgc
    15541 tgcttccggc cgcgcgtacc tttccgaaat acagtcccat gccgccgccg aatttgctga
    15601 cgcgggcaaa attgtcaatg cttctgtaaa ttccctccag actgtcggaa accgtatcaa
    15661 taaagcagct ggaaagctga tggtaaggct ttcttgcatt ggaaagcgta ggggtagcca
    15721 tagtcacata catccggctg agcatatcat aaaactttct cacccagtac aggcggtctt
    15781 ttttctcttc catggcaaga tggagggcaa ttcccaaaaa catctcctga ggcgtttcta
    15841 acggctcctg acggcggctt cggatcacat agcgtttaag cagcagatcc agtccggaat
    15901 aggtaaacag atgatccctt tccggacata aaaacgtctc cgcctcctgt atctcctcct
    15961 ttgtatagtg ttccaaaatg tattccccat agagtttttc ctctgtcaga taacaaagct
    16021 tgctgtaaaa atccgtgatt cccagcactt taagccgtcc tgacagctcc cgcctgaagg
    16081 atgctcccaa aagcctagct gcgatcatct cccactttgg agactgcgcg ctggtaagct
    16141 ccactgccgc ttttacgaga gccgcccaaa gctccggctc ttccattccc ggtctggcaa
    16201 agctgtggaa tttttcctcc agaagctcaa gactgtacgc ttcatccgta aaatcagaac
    16261 atattccctc taaaacggtt cttaagagcg cctgaagctc tgcatcctcc ttaaaaatct
    16321 gcattatcat ttccatatat catatccttt tcttttataa atcatcaatc agggccgtgc
    16381 tcttggcata agcggtgctc cgggcctcaa aaaaatccgt ctttaccaga tttgcattgg
    16441 aatactggct gacccaggcc atattttccg gctccttttc aaatccttca tagacagggt
    16501 caaagcccag gctcacccag cggagatttc ccagataata aatataatcc cggatcattt
    16561 cggctgtgag gccggaaata cgatccccga tcacatattc cccccacagg atttcctggc
    16621 ggacgccctc catcagaagt tccttcatca ttttcacatt tttgtctgta aacagctccg
    16681 gacgttcctg ctttaattcc cggatcatgt ttctgaacag ccacagatgg gtattttcat
    16741 cccgattgat ataacggatc tcctgcgcag aaccgggcat ctttccattg cggctcaaat
    16801 tgtaaaagaa catgaatccg gagtaaaaat agattccttc cagaatataa tttgccatca
    16861 ggacgcgcag aaaggcctca gggcttttat tctcctgaaa tccgttgtaa cattccccga
    16921 taaaggtatt gcgtcttaaa agatgctcat ctgtcttcca ctggtacaaa atagcgtcac
    16981 gctcaatggg actgcagatc gtatccagca tatagctgta gctctggctg tggatacatt
    17041 cctgaaaggt ctgtatattc agacaaaggt taatctcatt tgcagtaatg tattctccaa
    17101 tattgggaag attggctgtc tggatggaat ccaaaaacac cagaaagctt aaaatcttat
    17161 cataggcggc ccgttccgca ggggccagct tcggataatc cttcttatcc tgtcccagac
    17221 tgatttcctc aggaatccag aaattattca tggcctgacg gtaccagtcc ttgacccagg
    17281 tatagcgcat attgttaaaa tcattcagat ttgtggtatt tcccccgatc atgcgtcttg
    17341 cattaagact ggtatcaccc tggggattaa aaagcggctt tttcgtaagc tgatccatgg
    17401 atgttccttt cattttttaa tatcatattt attatcttaa catggactgt caatatatcc
    17461 tctttttgaa ttccctgcgt ttcctgaaaa aaacactggc aatctctatg aaagtaggct
    17521 ataatgggct gaatggtttt tcagaaagag ggtgacatta tgcacaaata tatgcgaacc
    17581 atcggattca gccagtatac ctcccgccag gatctggata agcttttaaa aaagcttgta
    17641 aaggacgctt ccaaatctga tattttaaaa gaatctgacg gagaaatgtt ctgcgaactg
    17701 cgggcacaga ccgctcccgg catgggcgtt gccatcgccg gacgtttaag ccagagagga
    17761 atctttaagc aggaatacta tttcccatat gtagaaagca gccaggtcac atccaccgcg
    17821 gactgctcga tccagcgcca tacggagcgt gaaacctttg caggcctttt agatgaatac
    17881 cgggtgggga tttctctgat cttttatatg gaaaattcga tggaataccg cctgcgcctt
    17941 aaaaacagcc agccgattct tccaaagagc gcttcgctct cggctctgtc cattcatgga
    18001 aaaatccttc ttccggtcca gaagacaccc cagcagacgg aagaccgaaa aaaagcggcc
    18061 agcaaacgca gccgtctcat cgaagccgcc aaacggggcg atgaagaggc catcgaaaca
    18121 ctgaccgtag aagatattga tcttttctcc tctgtatcac gccgtgtggc aagagaggat
    18181 atttattcca ttgtagacac ctgctttatg ccctccggca ttgagtgtga ccagtattct
    18241 gtcattggaa atattttatc tgtggaccgc aggatcaacc gcattaccgg agaagaaatc
    18301 tttcttctgg gcctggaatg caacgacctg ctctttacag ctgcaatcaa cgctgccgat
    18361 ctcttaggag agcctgttcc cggacgccgt tttaagggcc aggtatggat acagggaaat
    18421 gtatgctttg aagcctagtt ctattccgcc ttcactctct caaatatgta ccttgtatcg
    18481 gaagaaagct cctctctgaa ccgatatccc aatcggtcaa agctgcggat ttctgacgga
    18541 tcctctatgc ggttttccgc cataaaacga accatttccc cgcgggccat ttttgcatag
    18601 gttccctttg tcacaagctt tcctccggct tcttcacaaa agaccggagt gatatatctg
    18661 tcttcttcag aaagacagac ctcaatacat ttggaatact ctttggaagc cagattcagg
    18721 atgattccac tttcatctct tactgcctca tacagcctgt ttccccacag agcgtacaaa
    18781 tccttatatc cgccgacagc ggccctggcc tgcatctcca agcggtaagg cgtcacgccg
    18841 tccataggcc ttaaaacgcc gtaaaaagcc gacaggatgc gcagatgctc ctgaatatat
    18901 tcaaactgcc ctgtttcaaa tacagccgga gccatatact gataggcgat cacttcatag
    18961 gagagcactg ccggggtaag gttttttctc agatccatgt gggccagacg ttcaacgttc
    19021 tgccccacga tccggtcatt acagacccac agccgtttca gctcctcctg cgttcttgat
    19081 ctaagccagg ctaaaatctc ctccgtctga tccagaaatt cgggaagacc catatatgca
    19141 agcgtatccg gatcttcctt catcttttta gccggtgaca gtatgatcct cattccagct
    19201 cacccaacat cttttctgca tcttccgcag cctttacctt atcatttgca cggatgataa
    19261 ttccattttc atcaatcaga taggttgtac ggacgacgcc catggaaacc tttccatagt
    19321 tctttttttc cttccacaca tcataggcct caatgacctt gcgctccgga tcggacaaca
    19381 gagtaaatac aagaccgtac ttttcttcaa atttcttgtg agaagccact gtatctctgc
    19441 tcacgcctaa aacaacagtt cccttttcca taaactgggg ataccgctca gaaaatccgc
    19501 aggcctgttt ggtacagcct ggtgtgttat cctttgggta aaaatacagg attaccttct
    19561 ttcctctgta ttcttctaat gtgtgtattg ttccgttctg atccggaagt tcaaaattgg
    19621 gagctttgat tcctgtgttc agcatggtga gatcctcctt aaatatggta aatttttgtt
    19681 acgataactg ggttatggac atataataaa tagtttttat gggaaataca agtgaaattt
    19741 ctgtttcatc ctgtcatcat gctgactccg tcttcaaatt tcgatgaaag ctcagcttca
    19801 ccatataaat caaaaaactc cgcaccccct tacaaaaagt aaaggactac ggcgtcatct
    19861 tccagcgttc ccgaggtgac ttaaacaccc gacctaccgc ttaggagtac aagttgggag
    19921 ttattttgct ggcatccaaa tatttcccta tgttcgtgaa tgccttgatt ttacttgctt
    19981 ttatgttctt ctgtgtctga ataagaaaat tgaaatctgc ctgggtcaaa cgcatttcgg
    20041 gtggcaaaat ggtggcattg ccaccttggg gctttagcct ttgcgaaacc tctagttcat
    20101 acatatggct accattgtct tctgcaggca gcaatgataa cgtgatttcc ttattaaatt
    20161 actcaatatt atacagtgag tacagccgga tggcgatatg gttttgacta ctattgcaat
    20221 cttttcacct ttgtataaat taacaccagc tggtgcatga ttacattcta aagaagtttt
    20281 cgattccaat caataaaaaa catcttccac agatattgca ttaaaaatat ctaattttag
    20341 ttcttcatca gaatcatatg tacaggttag tattgcagca agaattctgc gaagcaaatc
    20401 ttcatcaata tgtccagctg ctacatgata actgatattc tccatttctc gaaagtatat
    20461 attagcaact gccatataac catttttaag caaagacatt gctgttaaag taatagctat
    20521 ccatttatta ccatcgcaaa agcagtgaaa tttacaagaa caataaaaca aatgcaaaag
    20581 cttatccaaa aaagtgggat agtaattatc gttttgaata tgacacaata cactttcaag
    20641 tttccctatg tcgagttcac cgcaatcgcc accaccacta ttccgaatgg ttgcaagatg
    20701 aatatcccgt gcttcatcta ttgaaatata gtgtatatcg gccatcaatt gcgctccttc
    20761 aatctcttca atacatcttg attctcatgc ataagcaatt caagttcatc gccagcgact
    20821 cctaagaatt tctcgtattc ttctgcttcc agaggcttaa tatactcttc caattgcata
    20881 tgaaatgcat cgcgtaaagc catatcccta ctagccattt tggtacgtgc tctaagaatc
    20941 aggggcttcc ataaaggtaa tgactcaaag gctgaaaaca aatctgacaa ttcccagtta
    21001 tttagttttc ttcccaactg cgtagactga tccttaatca ttgccgctaa tccacattcg
    21061 taaaacgcaa ttagatctaa gacctcagaa tacatcgtat ctctgacgct atctctttta
    21121 gacaaatgta aaacctgctt atattcctgg gctttttctc gaaaaatgct ttggtaaatt
    21181 ttatccgtat aaattgcata tttgctattt cccatggaaa catagtctct aagagcatca
    21241 gtaaattcac gtctatagtt ttcttcctgc aaaaacgcac tgataaaatc cctgtcacgt
    21301 tgattgatat attttgtcga accgcccgtc ctttgattaa cgaaatcaat aacaatatcc
    21361 aacatgatct gccggagaac ttttgcattt tcactttcaa ccaagagcat cgaaatattc
    21421 aaaaatgcct taaaatcaaa taatgcaatt tgaggagttc gattgctgat atgattatgc
    21481 acatgggctc gatccgtgtg cgttgccacg ataaaggcat actttccttt ggtaaaccga
    21541 agggccagct catgccccaa tcggtttgct tcctccggcg tgatctctcc cggtttgaag
    21601 gactagcgga tctgataggc gatcacttca tgcttttgcc gccgaccggt gatctgctca
    21661 tactgacgct tggacagcat aaactcttcg tccacggtca tcgggtcact ctgatagccg
    21721 gtaaccagtt cgcccttttc ggtcttttcc ggattctttg cataatccgt tctgtcaccc
    21781 agactctgag caatggtttt ccccttgttt acatgcaggg gaatgagtct tgttgctacc
    21841 atggtcatct gctcctttct cgaattttgg cataagaaaa gacctgagga gtttttttat
    21901 ttcccaggtc cggtatgtta taaactatta tgaaaaaatg aattgcaaaa gcttgcccgt
    21961 cagcgcttcc agtcccaaag agatcatttc agcaaacatc actgcccgga acaccagaac
    22021 tgcaaatcca aaaaccagtg cagcatagat ccagacgtgt tgaaacaaaa gcatcacaat
    22081 gcagacgacc agcaccagat ataaaaggcc gacgaccatt gcggaaaggt tcaatgcaag
    22141 ggagtaaaaa attgagaata cagcgacgat tgccataatc ggcagtgcga ttattctaaa
    22201 cgggagtttg atcaaccaca tatatacgtc ctccattgtc ggttctaata ataatttatg
    22261 aagcgattga tttcatgtca aggatgtcgc ttatgtatcc ggtgtgacag catacctgcc
    22321 acaccgtttt tactggatcg ttgccagtct ggcaaggatc tccttcgtaa tttcccggat
    22381 ctcgttctgc ctgttctgca aatctttgat atcctccgta tagatggagc cggtctcatt
    22441 ggctcgtttt gcgtactgat tcaggttgtt gctgcagtaa cggagaagcc cttctcgaga
    22501 ccgcatgtgc acctacagct ggagaagggc ataaaaaaat agccagagct gatgatcagt
    22561 cagttccagc tatgtaaatg tattcagttt gtcaacctct cttctatgct atttcaaacg
    22621 aatataacaa gtagattttc ccgtaccttc gcgttttaat tcaccggctg caaccaattt
    22681 tctcagtgca ccttcaacag aactaagact cagtgatgga cactgctccc tgatatcctg
    22741 ctttgtaaaa cgaccgattt tattcagggt ggcttttcgg accatatcaa tggcaggaag
    22801 tttctcctcc acgatagcaa accggtctcc aaagtcctta tatgcagcca gaatggtacc
    22861 cagcaaatat ttgatgaatg gaactgcatc ctcggttcct tcgtgccagc catgctgcga
    22921 acggcttagt gcctcatagt acaaatcctt gtttttcgcg atcttggctt ccagagaaat
    22981 gtatttgcta acgtagaacc cagagcgata caaaagcagt gtagtcaaca gacggctcat
    23041 tctcccgttt ccatcattaa agggatggat acataggaaa tcgtggatga aaaccggaac
    23101 tgcaatcaag ggctccagtt ccatatttcc gattaccctg ttgtactctt cacaaatccg
    23161 atccaatgcc ttcggcgttt cgaaaggcgc gaggggagtg aaaagaattt caatatgacc
    23221 atccggataa gtggcgctga tataattctg gacattcttt gtctgtccgg ccattggatt
    23281 attcatatgg ctgtacataa tcttgtgaag ctgcaggatg tagtttcttg tcagaggaat
    23341 ggcatcaaag ctttcgtgaa taatagaaag tacatcgcgg tatcccgcaa tttcctgctc
    23401 atctttgttc ctgggagtcg ttttctcctc aaccaactgc cggattcgtg tattggtggt
    23461 aacaataccc tcaatggcat tggatgcctc tgtactctga atctttgcaa tctcaaccag
    23521 cttctctaat tcctccgggc gctgcttcag gtaaagctcc tgttttccag cttccttata
    23581 gattgcggcg ataagcccca gaatctcaga atcccatttc tgctcccgga ttgttgagta
    23641 gttaaaatct ctcattccct taacctccat tctttccctt atattatatt caattttaat
    23701 ggatagtcaa ttaccttaaa taaaaattcc ctttatttct tttcaaaaat aaggggaaca
    23761 tgctttgata agggatagta tactctcatt tccggcgctt tattcatcac attgtatctg
    23821 aaatcagaga agaacagaaa aatagccaga actgatgacc agtcaattct ggctatgtaa
    23881 caggtgactt tcgccacccg gtcggatttt ctactccgac agcagttttg gatttaaccg
    23941 aagagtccag aaagcagatg taccagcgtg atacctatga gagccacgcc accaccgacc
    24001 atgagctgtt ttatccccaa ttaggagtaa acgttctgct cgatggcgga cggcggcgta
    24061 caaaaaatct atatggtgtt attaagagaa ccgtcccggc aggacaagtc aggcagtgac
    24121 ctgttccacc tccgggatgg gtttgagatt aaaatgaaaa gctgaaagaa aaagatatta
    24181 ttaaaatttt cccaacccag aagccccact gtactacaag gctggtgtta aagaccccaa
    24241 aaacggaaac aagtaatcgt accgtatggc tgccgacaac gcttgctcaa ttattggttc
    24301 aatacaagaa agaccagcag gaattgaaag agtttttagg aagtgcctat aacgattata
    24361 acctcgttat tgctcttgaa aatggaaatc ccgtagaaat ccgcattgtt cgggtcagat
    24421 ttacaacgct gtgtgaggaa cacaattttg aagttgttgt ttttcacagc ctacgccatt
    24481 taagcacaaa atataagctc aaaatgacac atggcgatat taagtctgtt cagggagata
    24541 caggtcacgc cgaagcagaa atggtaacag atgtttactc tgaaatcgtg gatgaagaca
    24601 gacggcttaa tgcaaagaag ctggatgaag aattttatga aaatggacgg acgttgtcct
    24661 attcccgccg agattggtgg ggtcaaaacc tgtccattac gcattaataa tccagattat
    24721 gtcgaaggcg gtgatatgcc taagacaatt gcaaatagct gtgacaagtg tatttattgt
    24781 gcaaagagac atgcctggca gtaactgtta tatataaaac aagggtgaaa agcctgattt
    24841 gtaagctttt cacccttcat tatcatagaa gaatctaatt tacagagatt ttctcatagt
    24901 ctccactttc caaagacaca agatttttta cagcctcttt tacttccacc aggctctgat
    24961 aaattctggt atagcagtct tttgcttttt ttaataagct ctctttatga tttcattcat
    25021 atgctgtgta gcttcaaagc gaattcgctc ttcatctaat gtaagtacct cacgattttt
    25081 cataacaagc tttccattga tgatggaatc gattacatca tgtccatttg cagcatctac
    25141 aatagtattt acaagattct gagttggtgt aatatgcggc tgatcaatat ttaaaagaat
    25201 cagatcagct ttctttcctt cttttacaga tcccagaata tcgccatgtc cgatagcatt
    25261 tactcctccc tgagaagcca ttttcaaaag agtcgggcag gtcataacta ccggattaaa
    25321 agaaggaagt ccccaataag ccagcataga ataacgcaga accttcatct catcgaaaat
    25381 atccaggttg gaaggagcag ctccatcaca acccaaacct acacttgcac cagcttccag
    25441 aatctgagga gccttcggga atccatgatt tgccaggttg gctcttggac aatggatcat
    25501 ttttactcct cgttctgcca tcaatgcaat atcatggtct gacagcataa cattatgtgc
    25561 ggtcagaaga tttggaccca aaattcccat ttcatctaaa aattcagcag gacgtttttg
    25621 ataattttgc aggcaaaagc taacttcatc tttatgctca cataaatgtg catggatgcc
    25681 tgtatgaagt tctttagcac attcacctac catggtgatg agatctctgg agcaggtcat
    25741 tacctgacga atagcaaacc agatatccac acggccatcg ccggctccct gatagttctt
    25801 atacagttct tttgtatggt ctatagcttc agatgcagtt tccttcattg cgcctgtaat
    25861 agcattgccc atatccattg tggatttcgc tattgcggct ctcatgccgg attctataac
    25921 tgcatcagct acacgctcca tatgtacacc gccggaatca gcaaaagcag tagttccgtt
    25981 tttgatcatt tccagacagg ctaactgtcc gctgacgtaa gaatcttccg ggagaagatt
    26041 gctttcaaag ggaaccagga aacgagtcca aaccatagga tattcatcag caacacgacc
    26101 tcttaacagc tgctgacagg tatgtgtatg accgtctatt aaacctggca taacaagctt
    26161 accttttcca tcaagtgttt ccgtggcaac actatcaacg aattcatctg ctgaaccgat
    26221 ttttttaatc caagtgtctt taattcctac ggaacaggtg gatgaaacgt tcatatcagg
    26281 attcagaacc tgacaatttt tgattaaaat atcatagttt tccataatct acctcatttc
    26341 atctcatatg attgttaaat ggctcctgtc attttcaaaa caatgtctgc aacaagagcg
    26401 gagaatataa aggttccggt aattacaaat aaagtgatca ataccagctt ccatcccatt
    26461 tttgtaaaat cctttagatc ttttcccaga gaaataccgg caaatgcacc aagtgctgta
    26521 gctggagcta aaaatgcaac aggagttgta gtttcaacta cgatatcttt taccggcgaa
    26581 acaggacatg caagaagcag cccgagaagg gatacataca tcatagaagg aagcttaaca
    26641 aatcggctca taaatttact tatagtaaga ccgataagag aaatagcact cagtaaaaga
    26701 actccaataa tggaatcttt tacaggcatt ttgtaaccaa ttacatttgt aagtattata
    26761 atcacagaca ccatgagaag cagtccaagc tgctgtacga agtattttct gaccatttat
    26821 ttaacctcct gattattttt gcgtccaatc ttaggctcta aaattgcgta taattttttt
    26881 gcaagaggaa ggcctacgaa agtacccatg taaattccgg taataccggt cagcatatca
    26941 ctggcaccac ccataatcat aatattttct gcctgctgtg ggtatatttc acctaatgtt
    27001 ccggcagcag cagtcatcaa ggacccactt cctactccac tggccatacc aagagcaata
    27061 ggatcgaaaa tattccagga agctacaacg cttgcaagaa tgctgataaa tactgttccg
    27121 attacagaac ctacgatata aactgcaaag gttccttttg tttccggagc gtccggtcca
    27181 aaaatatctg tagttaatcc aaggtttgaa tcacggttaa ttgagtaaca tgcaccaata
    27241 gattccttct ttaaaccaag caataatgcg agaggcatag ataaaagaat cgttccaagg
    27301 tttccgaatt cctgaagaag taatgcagga cccactgaaa ccagttttgc aacatttgca
    27361 ccggaagaaa tgcccatttt agccataaaa ggtgccaaaa ccacaaggac caggcttccg
    27421 cctattttac attcctcagt tttgaatact ttaaaaatat ccggccctaa aagccctccg
    27481 aatatgacgg caaacaccat cggaaacaga gtaagtgtac caacgccaat gttaagctta
    27541 acggttccga tcagatcaca gactgcggaa atgaccagag cggcaaggta aattttccag
    27601 tactttttga tgacaatctt tagtttttcc atataagaaa ctccttcctt tttttctgcg
    27661 caggtgcgta gtaatttacg ttgtaagtat aacatgagag ctggtttctg cgaacataca
    27721 aaaaagcgat gggaagccat cgcttttatc actatatcaa gaaaggtgtt ttctcactgc
    27781 attatttttt tataagaaaa tcgtcggatc tttatctaca taaaacagct tcatcctgca
    27841 ttaaaagctc cacaaaacgg ctggcggtat cagaaagtgt atgcttttga cgcaaaatcc
    27901 atcctatctc aaggcgaatt ccattttaga ataaggcgag actaaatgta cgtgatccaa
    27961 cctcaaacct gactttctca aatccagatc cccgatcttc aaccggtaga agccgttatc
    28021 caacggtata tcgatatcct ttgtctgtag ctgaagatca cctaattgaa acgtcttctt
    28081 cttcaaatcc acaaacacat tctccaaagc caattggata tccttttggc tctcagtccg
    28141 ca
//
LOCUS       DBMDUH010000015        26731 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_1277_length_26731_cov_7.566053, whole genome shotgun sequence.
ACCESSION   DBMDUH010000015 DBMDUH010000000
VERSION     DBMDUH010000015.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 26731)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 26731)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26731
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1277_length_26731_cov_7.566053"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1857
                     /locus_tag="ACZPCG_02685"
     CDS             <1..1857
                     /locus_tag="ACZPCG_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HIR1284009.1"
                     /translation="GFDVCFISEAESCGLLLFKKGSRQPVARIPFPHEGRMGNVHFMT
                     VKGEFEGLEYAFEEDGKEAADPFGRQFAGCGRWGHKERGTGGIHTVFPKKGADFDWED
                     DRLPAVPAQDCIIYKIHPRGFTKHASSGLSPEKRGTFEGVIEKISYLKELGITTLEMM
                     PPVEFNEIILILPEKSEIFGLPQGMRREEQEERPSHTEPVKLNYWGYCSGFYFAPKAS
                     YCSSGDPSAEFKRMVKALHHAGIELVIELYFDGSEAPGYVLDVVRFWAMEYHVDGVHL
                     VGFAPLSLLAEDPYLKNLKLWGEGWKEGDSRRICEYNSGFMMDMRRFLKGDEGMLSAL
                     SYHIRHNPDHMGVINYMANVNGFTMMDMVSYNEKHNEDNGEDNQDGSDQNQSWNCGAE
                     GPVRKKKVLSLRLRQLKNALLLLFLSQGTPLLLSGDEFGQTKQGNNNSYCQDNEISWL
                     NWGLIRRNKDLFAFVKQVISFRKSHDLFHMAKEPRLMDYRGLGLPDLSYHGEKAWQPE
                     FDRCSRQFGAFYCGDYSEDTEKTSFYTAYNMHWEPHEFALPNLPKGRAWQMVFNTAED
                     SVNGFWPEGKGPVLKDQKKVMIGARAVCVFMENPEVLPEKTVREKEGKKDAQ"
     gene            1862..2995
                     /locus_tag="ACZPCG_02690"
     CDS             1862..2995
                     /locus_tag="ACZPCG_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007035584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR1284010.1"
                     /translation="MEVFIREHEEEALELLRTIARIPAPSNHEEKRAEFCSRWLKEQG
                     AEGVYIDEALNVVWPAVIREKNGLEVYMAHTDVVFPDEEELPLKEEGDRIFCPGIGDD
                     TACLVCLLLGAKYLAEAIREGRLEEVYPSDRPGLLFVCNSGEEGLGNLKGVRNICEDY
                     AGRITAFCTFDSQLDKIVDQAVGSKRFKVTVRTKGGHSFNDFGRENAIERLAGIIGAL
                     YRIQIPKQGKTTFNVGQIQGGTSVNTIAQEAHMLYEFRSDRREHLDYMQDQFDKVIEQ
                     ARAQGMEIETEVLGIRPCSGSIDPERQRTLLERACMAAAQVTGQRPQTGSGSTDCNIP
                     MSLGIPSVCAGCYRGKGAHTREEYIEKSSISEGMKVVFGMIFG"
     gene            3133..5763
                     /locus_tag="ACZPCG_02695"
     CDS             3133..5763
                     /locus_tag="ACZPCG_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006774114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HIR1284011.1"
                     /translation="MTEWYSRSEEEVLRELSSCRRGLTDAEAHSRLEKYGENVLYERE
                     AVPWWQIFLSQFKDLLVVILMIAAGISMATGDPESALVIFTVLFLNALLGTIQHRKAE
                     KSLESLQKLSSPEARVVREGQSVTIPSSNVVPGDLMILEAGSLVPADGRLLEVYGLTV
                     NESSLTGESLSAEKHTGCLRTEGERLSPGDQVNMVFSGSLVTSGRAAAVVTATGMNTE
                     IGSIASLMNRAEVQKTPLQISLDHFSGHLALGIMGICVLVFGLSVYRKEPVLDALMFA
                     VALAVAAIPEALSSIVTIVQAMGTQKMAREHAIIKDLKAVESLGCVSVICSDKTGTLT
                     QNRMETEAVYINGREMETDQLKEYAGSGKKDAKLFLMAAALNNNTSPSAGDKEGDPVE
                     LALFHMVQAAGAVPEQLRLCCPRKGEIPFDSARKRMTTIHEVQGEEIMFVKGAPDVLL
                     ERCTRIINPAGADLVPSRQLSASDRAAILNQNQEWSLRGLRILAFACRSGAKWQEDPE
                     TDLVFLGLAAMMDPPRPESRPAVAAARQAGIRTVMITGDHRTTAMAVAGRIGIFLPGD
                     LALTGARLDQMTEEELEKKLPQISVYARVSPEHKIRIVKAWQNRGHIVAMTGDGVNDA
                     PALKKADIGIAMGMGGTEVSKDAASMILADDNFATIIKAAANGRNVYRNIKNAIQFLL
                     SGNMAGILCVLYTSLLGLPLPFAPVHLLFINLITDSLPAIAIGMEPAEDGLLKKPPRD
                     PKEGILTPGFVGQIFFQGGLMAGATMWAWSLGMRQGGEALACTMAFSALTLARLFHGF
                     NCRSSYSIIQLGLLSNPGSLAAFMLGSILLAAVLFVPGLQMFFAASDLMPVQAAAVGM
                     AAFLPTVVIQLGRIFRDAQQ"
     gene            5862..6758
                     /locus_tag="ACZPCG_02700"
     CDS             5862..6758
                     /locus_tag="ACZPCG_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR1284012.1"
                     /translation="MKFVTYEIEHKRRLGVINRDGTWIYPLRSLDMDYKTMQELIEGI
                     SESEKQLLEYVSGQDPYKIKGAVPAEEAILLAPIPHPVQDVICLGINYMAHAEESARY
                     KKEAFGGERPFAVYFSKRVSEATGTGAEIPSHKELVQDLDYEAELAVIIGKDAKNVPV
                     SEVKNYIFGYTVINDVSARTLQTRHKQWYFGKSLDGFLPMGPCIVTAEEFPYPPRLSI
                     QSRVNGELRQDSSTELMIFGIDHVVSELSAGMTLKAGTIIATGTPAGVGMGFDPPRFL
                     KPGDVVECSIEGIGTLVNRVSE"
     gene            6796..7260
                     /locus_tag="ACZPCG_02705"
     CDS             6796..7260
                     /locus_tag="ACZPCG_02705"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008723942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HIR1284013.1"
                     /translation="MDYLSMTIGKQKNIALIAHDQKKHELMDWCLEHKEALSKHFLCG
                     TGTTARMITEYTGLSVKGYNSGPLGGDQQIGAKIVEGKIDFVIFFSDPLTAQPHDPDV
                     KALLRIAQVYDIPIANNKASADFMIASPLMEEEYEHDVINFRQNISERAQTL"
     gene            7286..9772
                     /gene="glgB"
                     /locus_tag="ACZPCG_02710"
     CDS             7286..9772
                     /gene="glgB"
                     /locus_tag="ACZPCG_02710"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HIR1284014.1"
                     /translation="MEQVLYDLMDWAGIEELTYSESADPHRLLGSHITEKGLLIQALF
                     PTASAVKVKLSNTGKTYDMELADEAGFFAVLIPRKTKTAYTLLVTYDNGAEEEVTDPY
                     AFGPQYTERELKKFEAGIFYNIYEKMGAHPMTIDGVEGVYFSVWAPCAMRVSVVGDFN
                     LWDGRRHQMRKLGEGDASVFELFIPGLKPGCLYKYEVKTRAGEPMLKCDPYANYAELR
                     PNNASIVWDIGNYQWKDQEWMKKRAASDTKDKPFNIYEVHLGSWIRKAFAEDENGNVI
                     AGSEFYNYRELAVKLADYVKNMGYTHVELMPVMEHPLDASWGYQVTGYYAPTSRYGTP
                     DDFMYFMDYMHENGIGVILDWVPAHFPRDAYGMACFDGTCVYEHADPRQGSHPHWGTL
                     IYNYGRPGVSNFLIANALFWAEKYHADGIRMDAVASMLYLDYGKNDGEWVANMYGGNE
                     NLEAVEFLKHLNSVFKGRKDGAVLIAEESTAWPMITGNPKDGGLGFDYKWNMGWMNDF
                     TNYMRCDPYFRKNNYGGLTFSMLYAYSENFVLVFSHDEVVHGKGSMMGKMPGETLEKK
                     AENLRAAYGFMMSHPGKKLLFMGQDFGQIDEWNENASLEWELLQYPLHKNMQSYVRAL
                     NHMVLEHPALYEEDFDPSGFEWINCSYHEESMVLYVRRSKDGKETLLFICNFDNVEHE
                     KFRLGVPFAGKYKEIFNSDAREFGGQGRINARAKSSKKIPWDDRENSIECNIPPMSFL
                     VFSCTPEAGKKKAGRAAKSSVKEAAAKKEPETGGRKTAVVKEEPKKLSTAREEPKKLS
                     TTKEEPKKIVEAKSRERTQKTAQSFKRTES"
     gene            9831..11321
                     /locus_tag="ACZPCG_02715"
     CDS             9831..11321
                     /locus_tag="ACZPCG_02715"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1284015.1"
                     /translation="MLRNIWLTISIKKKIGIFAAMVILIMALSATFSICIMNFSLGGF
                     NTILNDNSRCHDFQEALDLEIESFADYIRDATPDTRDQYVLSCVRTERCLRSLPFDYA
                     RIGTERYARTWSILNGYETYQAYRDELAETQVRESDFVERLYRVYKMQEYLQTYARRL
                     VQVTLKEGNDSYQEKVPVFYNMPYLILAISAVFMGFVMFLTKILSNALVSPAVLLAQC
                     ARKIAKNDFTGEDPSVENRDEMGELVRAFNKMKRSAKGYIDTLKENHRMSELLHREEI
                     ERVEMEKQLSGARLELLKSQINPHFLFNTLNMIACMAKLEEAVTTERMISSMGSLFRY
                     NLKTSEQIVTLARELKVVQDYMYIQQMRFGSRILYSCDLKVDAEQAMIPAFTLQPVVE
                     NAMVHGLSKKEQGGRVHVRIWEQGNRLVISVADTGLGMSEERLAEVTEAMKERRTSRI
                     GIGLGNIYKRIHMMYKQGEFRIASIEGRGTVIQMFIPQEKHNRGDK"
     gene            11321..12109
                     /locus_tag="ACZPCG_02720"
     CDS             11321..12109
                     /locus_tag="ACZPCG_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006775092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1284016.1"
                     /translation="MYRMLIADDEMIERAVLYKMLNKNLEGQCEIYQAENGRQALELY
                     EKEKIQIAVLDIEMPGINGIQAAEKIREKDKDCVIIFLTAFDEFSYAKKAISVRAIDY
                     VLKPYDENELMLVIEEAMRMVEEQGQRKEMEQFFQNESEEADFEEDPVQPEDDNDHMS
                     RVGAMIRSFIEENYMYDISMQELARRMNYSEPYFCKLFKQYFDQNFTAYLTEYRIAEA
                     KKLLEQPTFNIKDVGKAVGYSDSNYFTKVFKRITGQSPTEYRNG"
     gene            12278..13411
                     /locus_tag="ACZPCG_02725"
     CDS             12278..13411
                     /locus_tag="ACZPCG_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR1284017.1"
                     /translation="MKKRWLSTALCAAMAAGTLAGCGLKTPETTTAAPAATEAAKEET
                     KAEEKTEAAEGETKAEAAASGKYDTANMPEVTLVYAEVNPLDTIVGQMATDFKEKVEE
                     LSDGKMTIDVQASGVLGSENDVLDTMLGGGGTIDMSRISAFALNGYGAKKSLLLSIPY
                     TFADREHFWNFATSDLADEFLAEPVEQGLGVRGLFYGEEGFRHFFFKEEANTLEDLKG
                     LKIRVSNDPVMTGMVAGLGANATVVSMNELYSALQTGVVDAAEQPIANYASNAFPEVA
                     PYLMLDGHTLGAVQVVITDEAWNGLTEDQQAILMEAGEYASANNRANSENKENEVLEQ
                     LKADGVTVVEVEDKAPWKEACKDVIESNTSDQAELYQQLLDME"
     gene            13550..14074
                     /locus_tag="ACZPCG_02730"
     CDS             13550..14074
                     /locus_tag="ACZPCG_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIR1284018.1"
                     /translation="MPEIFNKIDKIKPAYDLVYKAVMLICKLLLVADIVITSMSVVGR
                     YVSFIPDPAWSEEVVLTCMSYMAVLSAALAIRRGAHIRMTAFDNYLPKKVVTFLDILA
                     DVAVLVLAVIMITVGWKYATGIGSKGTYVSMPKVSRFWMYFPVPLAGVAMVVFEIEAL
                     YNHVKKIFVKEEAK"
     gene            14074..15366
                     /locus_tag="ACZPCG_02735"
     CDS             14074..15366
                     /locus_tag="ACZPCG_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR1284019.1"
                     /translation="MDANTFAILVLLGSFFLMIFLRFPIAYAVALSSVFCLVAQGHNL
                     TTICQQMVKGISSFSLMAVPFFITMGCLMGSGGISEKLIALANACVGWMRGGMAMVNI
                     VASYFFGGISGSASADTASLGSILIPMMVDQGYDGDFSTAVTITSSCEGLLVPPSHNM
                     VIYATTAGGISVGSLFLAGYIPGALLAASLMIGSYIISVKRNYPKGDKFSMKVLLKQF
                     SVSFWALAAVLIVVVGVVGGVFTATESAAIAVIYSLLVSVFIYKGLDWKGVWRVLGDC
                     IDTLSIVLILIATSSIFGYCLTQLHVPDLAATAITNFTNNPIVLALLLNLILLVLGCI
                     MDMAPIILIATPILLPIATSIGIDPIQFGIMVVLNCGIGLLTPPVGSVLFIGSAIAKL
                     PMEKVVKATLPFYLCMIITLLLITFVPAISLWLPQVLA"
     gene            15454..15930
                     /locus_tag="ACZPCG_02740"
     CDS             15454..15930
                     /locus_tag="ACZPCG_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HIR1284020.1"
                     /translation="MYQYTYRLSNGEYWRLSMYYTYGSMVGVCNLIFTAALFILTYVK
                     WGGAGWGYRAMLILGCSLFTVLQPLAIWRRSKKIASAIKEDTHIMIDGKGIHVTVGTA
                     HTDIVWDKIRRISKKPDMLVIFSDTTHGYVLTDRVLGGDREAVYSFITARMNHGKK"
     gene            15986..16960
                     /locus_tag="ACZPCG_02745"
     CDS             15986..16960
                     /locus_tag="ACZPCG_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007035593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR1284021.1"
                     /translation="MSASVWGCQSREKGAGEENVPEFVLTYAENQAEDYPTTQGAYRF
                     AELVKQRTGGKVEILINAEGILGDERTVIEQLQFGGVDFARVSLSPLSEFVPELNVLQ
                     LPYLYTGAEHMWKVLEGEIGDSFLGSFEGSSLVALSWYDAGARNFYNSVRPIEKLEDM
                     KGLTIRVQESELMSDSIRVLGAVPKQMAYGEVYSGLQTGEIDGAENNWPSYESTRHYE
                     VARYYTIDEHTRVPELQLAAQSTWDKLPEEYRIIITQCAKESARYERSLWKEREAQSE
                     QNIREAGCIITELSKEEKSRFQAAVMPMYGKYCSDYVDMIDRIVAEGR"
     gene            17084..17689
                     /locus_tag="ACZPCG_02750"
     CDS             17084..17689
                     /locus_tag="ACZPCG_02750"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HIR1284022.1"
                     /translation="MNKVITVSREFGSGGRELGVKLADALGIPFYDKELISMAADDIN
                     IAEEAFRRYDEHIVVEDPLDRNFYHAFSDIYQVPMSDQIFVAQSNVIRRLASHGPCII
                     VGRCADMVLEDSINIFVYAKMKNRIRRMMELEPGSDEKEMERRIREVDRKRKDYYQYY
                     TGNTWGKAQNYHLCVDSGLVGVDGCLRAVTAYLGELCRDLS"
     gene            17783..18868
                     /locus_tag="ACZPCG_02755"
     CDS             17783..18868
                     /locus_tag="ACZPCG_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR1284023.1"
                     /translation="MEKPGKKLKKFLIITGTTGLVYAGLKYLLPLVAPFFIGYTLALI
                     LRPSVRFIRYRFQFSAFGKTWKIPSGLIGAAEIGIIFGGLGIWVWQGILRLCRELQLL
                     AVRLPLWIMDFDSRMAEYCMKMERAAGLKSGCLFETAAEMTATLGEKAKESLMPFLMT
                     NSLPAAAFFIRLTLVCLVSFLAAVLSLQEMEELRHRRDQSLFRREFNLIGSRLVQTGK
                     AWFKSQGIILLLTCGLSIGAMIWMGNPYAVLAGTAIALLDALPLLGAGAVLLPWAFLC
                     VLGGQWKDALILSALYLACYFARQYLEAKYMGSEVGLSPLETLAAVYVGMELFGFLGF
                     ILGPLGLLLIEDLTEIWEEGADRGKLT"
     gene            18905..19669
                     /locus_tag="ACZPCG_02760"
     CDS             18905..19669
                     /locus_tag="ACZPCG_02760"
                     /inference="COORDINATES: protein motif:HMM:NF013539.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HIR1284024.1"
                     /translation="MMGEDMKMFDVEQVKALNDLERMVYEYVVKDPEKVKYMRIRELA
                     DEVHVSTSTILRFCKKMGCSGYSEFKLQLRDYQTRKDQANVRDDISEVVDFLKKTAAQ
                     EFEDKLEMLAEVIFHANHVFLVGSGMSGIVAKYGARYFSSMGKFCLYIDEPHYPTESS
                     FLKDSLVIVFSVSGESGDTIRHVMRFKEQNCRILSITNTENCTIAKLSDHNIAYYVSY
                     MRLGDYDVTTQIPAVSIVERLGKKLYRYTSKGPGAI"
     gene            19802..21208
                     /gene="ascF"
                     /locus_tag="ACZPCG_02765"
     CDS             19802..21208
                     /gene="ascF"
                     /locus_tag="ACZPCG_02765"
                     /inference="COORDINATES: protein motif:HMM:NF007311.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HIR1284025.1"
                     /translation="MAKDYTVISRKIVEQIGGIDNIANVTHCMTRLRFVLKDETLADD
                     EAVKAIDGVMGLVKQGGQYQIIIGNHVGTAYQEVLKLGNFGEQSEIAKGEKKEPLTLK
                     KVGNNILDAIVGTMSPLIPAIIGGSMVKLLVMLLGMAGILSADSETYKLINTIGDGAF
                     YFLPMLVAASAAKKFKTDMFLAISIAGVLIHPDFRSMMEAVTVGDTAAHFMGIPIASV
                     KYTYTVIPSICMTWILSYIERGVDKITPAVTKNFLKPMLILLIAAPIAILIIGPAGIY
                     VGEGISSFVFAVQDKLGFVAVGIMGAIWPLLVITGMHRVFTPTILQTISETGMEGTVM
                     PSEIGANLSLGGVSLAVALKTKNSELRQTALAAASSALIAGTTEPALYGVAVRLKRPL
                     LASLITGFVCGCLAGIGGLASRSMVSPSVLTGVQFIDPANPGRSIAWIAGITILSIVL
                     SFVLTLVIGFEDIPEEEK"
     gene            21211..22638
                     /gene="ascB"
                     /locus_tag="ACZPCG_02770"
     CDS             21211..22638
                     /gene="ascB"
                     /locus_tag="ACZPCG_02770"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HIR1284026.1"
                     /translation="MSDFRVPENFLWGGAISANQAEGAYREGGRGISNIDLIPHGSSR
                     MDVKLGKVSDPELQTDAFYPSHEAIDFYHHYKEDLALMGELGLKVFRTSISWSRLFPE
                     GDEAFPNPEGIAFYKDLFKECRRYGMEPLVTLCHFDVPYGLIRKYGSWRSREVIGCFV
                     RYAKTCFRELKGLVKYWLTFNEINIVLHSPFSGAGIAFAEGENRNQVIYQAAHHMLVA
                     SALVTKAAHETDPENKVGCMLAGGSFYPYSCNPEDVWESVKKEQENLFFIDVQVRGHY
                     PSYAKKLFREKGICLNMEPEDSDILKNTVDFMSFSYYSSRCVAADMADKTSNEGNVVK
                     SVKNPWLKTSQWGWAIDPLGLRITMNQLYDRYQKPLFIVENGLGARDVLTPEGTVEDD
                     YRIDYLKKHIEAMKLAMEDGVELMGYIVWSCIDLVSASTGEMSKRYGFVYVDRDDEGK
                     GSLKRQKKKSFYWYQDVIRSNGEYL"
     regulatory      22768..22864
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            23144..24499
                     /locus_tag="ACZPCG_02775"
     CDS             23144..24499
                     /locus_tag="ACZPCG_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015851 - nucleobase transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HIR1284027.1"
                     /translation="MRTEVLAGITTFITVAYILILNPQILADPFVIGGDMDMASKISN
                     GVFIGTCIGAFIGTILCAVYAKVPLAQAPGMGLNAFFAYTVVLGMGYTYHQTLTIVFI
                     SGVLFIVITAVGLRQAIIRAIPEQIKMAITPGIGLFITIVGLKNAGLVVSNPSTLVSM
                     VDFAQWRNGVDTQLICGALVALIGLIVIGVLHTKNVKGSILWGIVAATVVGIPLGVTK
                     LSAFDLDLAAKFRDFGEVSLFKMDVAGLFEGKSVTDAIFTIIMLVLSFSLVNMFDSMG
                     TLMGAGRQSGLIDENGEVIHMQEALMADAISTAAGALVGTSTVTTVVESSAGIAAGGR
                     TGMTSLVTAALFLGAIIFAPVVSIVPAAATAPALIFVGVLMLSNIKDVDFSDMTNGLP
                     AFCTVVFMPFTYSIANGIAMGLITYCLLKILARRPKEVEVLTGLIAVVFVFRYAFMTL
                     G"
     gene            24527..25807
                     /locus_tag="ACZPCG_02780"
     CDS             24527..25807
                     /locus_tag="ACZPCG_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006781263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR1284028.1"
                     /translation="MEQKTFALKGNIIYTKERTLFEVMEQGWLVCEEGRVRDVYSVLP
                     EELKGIPVEDMGDRLIIPGLTDLHIHAPQYTFRSLGMDMELLEWLETNTFPEESKYKD
                     AEYAQKAYGMFAEQLKKSATVRACIFGTIHNEATLILMDLLEEAGLHTMVGKVNMDRN
                     SPDYLCEPSAEASAQATREWLKAVADRKYKHTRPILTPRFTPSCTDELMELLKEIQTE
                     THLPMQSHLSENPGEIAWVKELCPWSEFYGDAYDKFGLFGGDCPTVMAHCVYSGKEEM
                     ALMKEKGVFVAHCPESNTNLSSGVAPVRRYLEEGLHVGLGSDVAGGSTENLFTAMSQA
                     IQASKLRWRLQDDSLKPLTAEEVFYMASKGGGAFFGKTGSFEPGYEFDAVIIDDSRLI
                     HPQPLTVKQRLERVIYLGDEREVYGKYACGEKIL"
     gene            25912..26493
                     /locus_tag="ACZPCG_02785"
     CDS             25912..26493
                     /locus_tag="ACZPCG_02785"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016296757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HIR1284029.1"
                     /translation="MEWKDGKCRCCWANPKNERYIRYHDEEWGRSVHDDRKLFEMLVL
                     ECFQAGLSWECILNKREAFKQAFDEFELETVCGYGEEKLQELKENAGIVRNSLKIIAA
                     VTNARIFRKIQAEYGSFDCYLWHWTEGKVICETGKTNSALSDRISEDLKKRGMKFVGT
                     TVVYAYLQAVGVVWGHEENCFLSLHQGKNMQKK"
BASE COUNT         7093 a         5435 c         7384 g         6819 t
ORIGIN      
        1 ggctttgatg tgtgttttat ttcggaggca gaaagctgcg ggctgcttct cttcaaaaaa
       61 gggagcaggc agcctgtcgc cagaatcccc ttcccccatg agggacggat gggaaatgta
      121 cactttatga ccgtaaaggg agagtttgag ggtctggaat acgcctttga ggaggatgga
      181 aaggaggcag cagatccgtt tggacggcag tttgcaggct gcggacgctg gggccataaa
      241 gaacggggaa caggcgggat ccatacagta tttccgaaaa aaggtgcgga ttttgactgg
      301 gaagatgacc gtcttccggc tgttcctgct caggactgca tcatttacaa gatccatccc
      361 agaggtttta caaagcatgc ttcttctggt ttgagtccgg aaaagagggg aacctttgaa
      421 ggagtaattg aaaagatttc ctatcttaaa gagctgggga ttaccacgtt ggaaatgatg
      481 cctccggtgg aatttaatga gattatcctt atccttccgg aaaagtccga aattttcggt
      541 cttccccagg gaatgcggag ggaagaacag gaagaaaggc cttctcatac agagcctgta
      601 aagctcaatt actggggcta ctgcagcggc ttttattttg ctcccaaggc ctcctattgt
      661 tcatccgggg atccgtcggc agagtttaaa cggatggtaa aggctcttca ccatgcaggg
      721 attgaactgg tgatcgaact gtattttgac ggaagtgaag cgccgggata tgtgctggat
      781 gtggtgcgct tctgggcgat ggaataccat gtagacggcg ttcatctggt aggttttgca
      841 cctctttccc tgctggcgga agacccttat ttaaagaatt taaagctgtg gggagaaggc
      901 tggaaggagg gggattcccg ccgcatctgt gagtataatt ccggctttat gatggatatg
      961 agacgctttt taaagggcga tgaggggatg ttaagtgcgc tgtcctacca tatccgccac
     1021 aatccggatc atatgggagt catcaattac atggcaaatg taaacggctt taccatgatg
     1081 gacatggttt cctataacga aaagcataat gaggacaatg gggaagataa tcaggacggc
     1141 tcagaccaga atcagtcctg gaactgcggg gcagaggggc cggtgagaaa gaaaaaagtt
     1201 ctcagtctgc ggcttcgtca gttaaagaat gccctgcttc ttctgttttt aagccaggga
     1261 acgccccttc ttctatctgg tgatgagttt gggcagacca aacagggaaa taacaattcc
     1321 tactgtcagg acaatgaaat ttcctggctg aactgggggc ttatacggcg taataaggat
     1381 ctatttgcct ttgtaaaaca ggtgatctca ttcagaaagt cccatgatct gttccatatg
     1441 gcgaaagaac ccagactgat ggattaccgg gggctgggac tgccggatct gtcctatcac
     1501 ggagagaagg catggcagcc ggaatttgac cggtgcagcc gtcagttcgg cgcattttac
     1561 tgcggcgatt attcagagga tacagaaaaa accagctttt atacagccta taatatgcac
     1621 tgggagccac atgaatttgc gctgccgaat ctccccaaag gaagggcatg gcagatggtt
     1681 ttcaatacag ccgaggacag tgtaaacggt ttctggccgg agggaaaagg ccctgtatta
     1741 aaggatcaga aaaaggttat gatcggagcg cgggcagtct gtgtatttat ggagaacccg
     1801 gaggttctgc ctgaaaaaac ggtccgggag aaagaaggaa aaaaagatgc tcagtgagtc
     1861 aatggaagtg tttatcagag aacatgagga ggaggccctg gaacttttaa gaactattgc
     1921 caggattcct gcgccctcaa accatgagga aaaacgggcg gaattctgtt cccggtggtt
     1981 aaaagagcag ggggcagaag gggtatatat agatgaggca ttaaatgttg tctggccggc
     2041 cgtgatccgt gaaaaaaatg gcctggaggt ctatatggcc catacggatg tggtatttcc
     2101 tgatgaggag gagttgcctt taaaagagga aggagaccgt atcttctgtc cgggcattgg
     2161 ggatgataca gcctgtcttg tctgcctgct ccttggggca aagtatctgg cagaggccat
     2221 ccgggaaggg cgccttgagg aggtgtatcc ctctgaccgc ccggggcttt tgtttgtatg
     2281 caattccggg gaagaagggc tgggaaatct aaagggcgtc cggaacatat gcgaggatta
     2341 tgccggtcgg atcacagctt tttgtacctt tgacagccag ctggataaga tcgtagacca
     2401 ggctgtggga tctaagcgat ttaaggtcac agtgcgcacg aaaggaggac actcctttaa
     2461 tgatttcggc cgggaaaatg ccattgaaag gctggcaggc atcattggag cactgtaccg
     2521 gatccagatt cctaagcagg gaaaaacgac ttttaatgta ggccagatcc agggcggaac
     2581 atcggttaat accattgctc aggaggctca tatgctttat gagttccgtt cagaccggag
     2641 ggaacatctg gactatatgc aggatcagtt tgataaagtg atcgaacagg ccagagcgca
     2701 ggggatggag atcgaaacgg aggttttagg gattcgtccc tgctccggca gcattgaccc
     2761 ggagcgccag aggactcttt tagagcgggc ctgcatggct gcagcgcagg ttacagggca
     2821 gcgcccccag acaggctccg gttccacaga ctgcaatatc cccatgtcgt tgggaatccc
     2881 cagcgtctgc gcgggctgct accggggcaa aggcgctcat accagagagg aatacattga
     2941 aaaatcctcc atatccgagg ggatgaaggt cgtatttggc atgatatttg gctgatgtca
     3001 taacagacct ccctttttca taaattaaac agagtgtaca gaccggatgc ttccggggcc
     3061 gctcaaggat ggtcccggga gcatttttcg ttgcggtttt tacagatcca gagtataaaa
     3121 gggggagccg gaatgacaga atggtacagc agatcagagg aagaggtttt aagggagctt
     3181 tcctcctgcc gccgggggct gaccgatgca gaggcccatt ccaggctgga aaaatatggg
     3241 gaaaatgttc tatatgagcg ggaggctgta ccctggtggc agatttttct aagtcagttt
     3301 aaggatcttc tggtagtgat cttaatgatc gctgccggga tatccatggc tacaggagat
     3361 ccggagagcg ctctggttat ttttacggtt ttgtttttaa atgccctgtt gggaacgatc
     3421 cagcacagga aggcggaaaa atccctggaa agccttcaaa agctgtcctc tcctgaggcc
     3481 agggtagtga gagagggaca gtcggttacc attccttcct cgaacgtagt gccgggagac
     3541 ctgatgattt tagaggccgg aagcctggta ccggcagatg gccggctgct ggaagtttat
     3601 ggacttacgg tgaatgaaag ctctcttacc ggagaatcgc tcagcgcaga aaaacatacc
     3661 gggtgtctga gaacggaagg ggagcggctg tctcccggag atcaggtcaa tatggtattt
     3721 tccggttctc tggttacctc cgggcgggct gccgcagtag ttacagccac aggaatgaat
     3781 acggagatcg gttcgatcgc ttctttgatg aaccgggcag aggtacagaa aacacccctg
     3841 cagatcagcc tggaccattt ttccggtcat ctggcgcttg gcattatggg aatctgtgtt
     3901 ttggtttttg gactcagtgt ttaccggaag gaacctgtac tggacgcgtt gatgtttgct
     3961 gtggctctgg ctgtggcggc aatcccggaa gcattaagct ccattgttac cattgtccag
     4021 gccatgggaa cccagaaaat ggcaagggag cacgcgatca tcaaggattt aaaggcagtg
     4081 gaaagccttg gctgcgtatc tgtgatctgc tctgataaga cgggaacgct gactcagaac
     4141 cgtatggaaa cagaggcggt ctacatcaat ggaagagaaa tggagacgga tcagctgaaa
     4201 gagtatgcgg gatcaggaaa aaaggatgcg aagctctttt taatggcagc cgctttaaat
     4261 aataatacat ctccatctgc cggggataag gagggggatc cggtggagct ggccctgttt
     4321 catatggttc aggctgccgg agcagtcccg gaacagctgc gcctgtgctg tccaagaaaa
     4381 ggggagattc cctttgattc tgcaagaaag agaatgacga ccatccatga ggtgcagggg
     4441 gaagaaatca tgtttgttaa gggtgctccg gatgtgctgc tggagcgttg cacacggatc
     4501 ataaatcctg ccggagcgga tcttgttccg tcacgacagc tttctgcttc cgaccgggcg
     4561 gcgattttaa atcagaatca ggaatggtcc ttacgggggc tcagaatttt ggcatttgcc
     4621 tgccggtccg gagccaaatg gcaggaggat ccggaaacag accttgtgtt tctggggctt
     4681 gcggcaatga tggatccgcc ccggccggag tcacgcccgg cagtggctgc ggcccgtcag
     4741 gccggaatac gcacggtgat gatcacagga gatcaccgga caacggcaat ggctgtagca
     4801 ggccggatcg gcattttttt gcccggagat ctggcgctta caggagcccg gctggatcag
     4861 atgacagaag aagaactgga aaaaaagctg ccgcagattt ctgtttatgc ccgcgtatct
     4921 ccggagcata aaatacgcat tgtaaaagca tggcagaacc gggggcatat cgtagccatg
     4981 acgggcgacg gggtaaatga tgctcccgcc ctgaaaaaag cagatatcgg cattgctatg
     5041 ggtatgggag gaacagaggt gtcaaaggat gccgcctcca tgattctggc ggatgacaat
     5101 tttgctacga ttatcaaggc tgctgccaat ggaagaaacg tataccgcaa tattaaaaat
     5161 gccatccagt ttcttttgtc cggaaatatg gcagggattc tgtgcgtcct ttacacttcg
     5221 cttttggggc ttccccttcc ctttgctccg gttcatctgc tgtttatcaa cctgatcacc
     5281 gattctctcc cggcaatcgc cattgggatg gagccggcag aggatggact gcttaagaaa
     5341 ccgcccagag acccgaagga agggattttg actcccggct ttgtcggaca gatctttttt
     5401 cagggcggcc tgatggcggg agcaaccatg tgggcctgga gcctgggaat gagacagggg
     5461 ggagaagctc tggcctgtac catggcattt tctgcgctta cactggcgcg tctgtttcac
     5521 ggctttaact gccgcagcag ctattcgatc atacagcttg gccttctcag caatccgggc
     5581 agtctggcgg catttatgct tgggagcatt cttttggcag cggttctttt tgtgccgggg
     5641 ctgcagatgt tttttgccgc atccgatctc atgccggttc aggcggcggc tgtaggaatg
     5701 gctgcatttc ttcctacagt ggtgatacag ctgggaagga ttttcaggga tgcgcagcag
     5761 taagagactg aaaaaataga aatcagagcc gaaaagtaga aaaaatcgga aaaatgtggt
     5821 acagtagaca ataagtgagt gcagaataca ggaggccgtc aatgaaattt gttacatatg
     5881 agattgaaca taagagaaga ctgggcgtta taaacaggga tggaacatgg atctatccgt
     5941 tgcgctctct ggatatggat tataaaacca tgcaggagct gatcgagggc atcagtgagt
     6001 ctgaaaagca gcttctggag tatgtgtcag gacaggatcc ttataagatc aaaggggcag
     6061 ttcccgcaga ggaggctatc cttcttgcgc ctattcctca tcctgtgcag gatgtaatct
     6121 gcctgggcat caattatatg gcccatgcag aggaatcggc ccgctataag aaagaggcct
     6181 ttggcggaga gcgccccttt gccgtatatt tctcaaaacg ggtcagcgaa gctacgggta
     6241 caggcgctga aatccccagt cataaggagt tagtgcagga tttggattac gaagctgaac
     6301 tggcggttat tattggaaag gacgcaaaaa atgtgcctgt ctccgaggtg aaaaattata
     6361 ttttcggtta tacggtgatc aatgatgtga gcgccagaac ccttcagacc cgtcataagc
     6421 agtggtattt cggcaaaagc ctggatggat ttcttcccat ggggccttgt attgttaccg
     6481 cggaggaatt tccctatccg cccaggcttt ccattcagtc cagggtaaac ggagagcttc
     6541 gccaggacag cagtacagag cttatgatat ttggcattga ccatgtggta agcgagcttt
     6601 ctgccggaat gaccctgaag gcaggcacca ttatcgctac aggaacgccg gccggtgtgg
     6661 gaatgggctt tgatccgccc cgttttttaa agcccgggga tgtggtggag tgctccattg
     6721 agggaatagg aaccctggta aaccgggttt cagagtagaa aagaaacagg cacagcataa
     6781 aaggaggccg ggaaaatgga ttacttatca atgaccattg gaaaacagaa aaacattgcg
     6841 ttgattgcac acgaccagaa aaagcatgaa ctgatggact ggtgtctgga gcataaggaa
     6901 gcactgtcaa agcattttct gtgcgggaca ggcaccacag ccaggatgat tacagagtat
     6961 acgggccttt cggtaaaggg atacaacagc gggcccttag gcggcgacca acagatcgga
     7021 gccaagattg tggaaggaaa gattgatttt gtgattttct tttccgatcc tctcacggcc
     7081 cagcctcacg atccggatgt gaaggcgctt cttcggatcg cacaggttta tgacattccc
     7141 attgcaaaca ataaagcaag cgcggatttt atgattgctt ctcctctgat ggaagaggag
     7201 tatgagcatg atgtgatcaa tttcaggcaa aatatttcag aacgggctca gacattgtag
     7261 ccggcagaag ggggaagctg tatttatgga acaggtatta tacgatttga tggattgggc
     7321 gggaattgag gagctgactt attcggagtc tgcagatcct cacagacttc tgggatctca
     7381 cataacagaa aaggggcttt tgatccaggc tctgtttcct acggcttcgg ctgtaaaggt
     7441 aaagctttcc aatacgggaa agacatatga tatggagctg gcagatgaag caggcttttt
     7501 tgcagtcctc attcccagaa agacgaaaac agcctatacg cttttagtga cctatgataa
     7561 tggggcggag gaagaagtta cagatcctta cgcctttggc cctcagtata cggagcggga
     7621 gctcaagaag tttgaagcgg gaattttcta taacatctat gagaaaatgg gtgctcatcc
     7681 catgacaata gacggtgtgg agggtgtata tttttccgta tgggcccctt gtgccatgag
     7741 agtcagcgtg gtaggagatt ttaacctctg ggacggcaga cgccatcaga tgaggaagct
     7801 gggggaggga gatgcaagtg tatttgagct gttcattccc ggcttaaaac caggctgcct
     7861 ttataagtat gaggtgaaaa cgcgggccgg ggaacccatg ttaaaatgtg atccctatgc
     7921 caactatgca gagctgcgcc ccaataatgc ctccattgtg tgggatattg gaaactatca
     7981 gtggaaggat caggagtgga tgaaaaagag ggccgcatct gatacaaagg acaagccgtt
     8041 taatatctac gaagttcatc tgggatcctg gatccgcaag gcgtttgcag aggatgaaaa
     8101 tggaaatgtg attgccgggt ctgagttcta taattaccgg gaattggctg taaagctggc
     8161 tgattatgta aagaatatgg gatataccca tgtggaactg atgccggtga tggagcatcc
     8221 cttagacgct tcctggggat atcaggtgac agggtattat gcgcccacca gccgatacgg
     8281 aaccccggat gatttcatgt attttatgga ttatatgcat gaaaacggca tcggggtgat
     8341 ccttgactgg gtaccggctc atttccccag agatgcctat ggcatggcct gctttgacgg
     8401 tacctgtgta tatgaacacg cagatcccag acagggttct cacccccact ggggaactct
     8461 gatctacaat tacggccgtc ccggcgtatc caatttcctg atcgcaaacg ccttattctg
     8521 ggctgaaaaa tatcacgcag acggtatccg aatggatgca gttgcttcca tgctgtatct
     8581 ggactacggc aaaaatgacg gcgagtgggt agccaatatg tacggcggaa atgaaaatct
     8641 ggaagccgta gaattcttaa agcatttaaa cagcgtattt aagggacgca aagacggcgc
     8701 tgtgctgatc gcagaggagt ccacagcatg gcccatgatc accggcaatc caaaggacgg
     8761 cggtcttggc tttgactaca agtggaatat gggttggatg aatgacttca ccaactatat
     8821 gcgctgcgat ccctatttcc ggaaaaataa ttacggcggg cttacctttt ccatgctcta
     8881 tgcctacagt gaaaactttg tcctggtatt ttcccatgac gaggtggttc acggaaaagg
     8941 ctccatgatg gggaaaatgc caggagaaac tctggaaaag aaagcagaaa atctgcgcgc
     9001 tgcgtacggc tttatgatgt cccatccggg caagaagctg ctctttatgg gacaggactt
     9061 tggccagatt gacgagtgga atgaaaatgc ttctctggaa tgggaacttt tgcagtatcc
     9121 tcttcacaaa aatatgcaga gctatgtaag ggcattaaac cacatggttc tggagcatcc
     9181 ggctctttac gaggaggact ttgaccccag tggatttgag tggatcaact gcagctatca
     9241 tgaagaaagc atggttttat atgtacgcag aagcaaagac ggaaaggaaa cgctgctctt
     9301 tatctgcaac tttgataatg tggagcatga gaaattccgt ctgggagtac cgtttgcagg
     9361 caaatataag gaaatcttca acagtgacgc cagggaattc ggcggtcagg gcaggatcaa
     9421 cgcccgcgca aagagctcga agaagattcc atgggatgac agagagaact ccatcgaatg
     9481 caatatcccg ccaatgagct ttctggtatt ttcctgtacg ccggaagctg gcaaaaagaa
     9541 ggccgggagg gctgcaaaaa gctctgttaa ggaggctgcc gcaaagaagg agccagagac
     9601 cggcggaaga aagacagccg tggtgaaaga ggagccgaag aagctgtcta cagccaggga
     9661 agaacctaaa aaactttcta ctacaaaaga ggaacctaaa aagatagtgg aggcaaagag
     9721 ccgggaaaga acccaaaaga cggctcaatc tttcaaacgc actgagtcgt aaaacacagg
     9781 aggggccgag gatacagaca gaaaggaaac gcagattata atacagatct atgcttcgta
     9841 atatatggtt aacgatttcc atcaaaaaga agatcggtat ttttgcagcc atggtcattt
     9901 tgatcatggc tctttcggct acgttcagta tttgtattat gaacttctcg ctgggaggct
     9961 tcaataccat tttaaatgac aattcccgct gccatgattt tcaggaggca ctggatcttg
    10021 agattgagtc ctttgcggat tatatccgcg atgccacacc tgatacgaga gatcagtatg
    10081 ttctgtcctg cgtccgtaca gagcgctgtc tccgctccct tccctttgac tatgcccgaa
    10141 ttggcacaga gcgctacgcc cgcacctgga gtattttaaa tggttatgaa acctatcagg
    10201 cctatcggga tgagcttgct gagacacagg taagggaaag cgattttgtg gaacggctgt
    10261 accgggtgta caagatgcag gaatatcttc agacctatgc cagacgtctg gtgcaggtca
    10321 ctttaaaaga aggaaatgac agttatcagg aaaaagtacc tgtattttat aacatgccct
    10381 atctgattct ggctatttcc gccgtcttta tgggttttgt gatgttttta accaaaatcc
    10441 tctccaacgc gctggtatct ccggcagtcc ttctggccca gtgcgccaga aaaatcgcca
    10501 aaaatgattt taccggagaa gatccctctg tagaaaaccg ggatgaaatg ggcgaactgg
    10561 tgcgggcatt taacaaaatg aaacggtctg caaaggggta tattgataca ttaaaggaga
    10621 atcaccgtat gtccgaactg ctccacaggg aagagatcga aagggtagag atggaaaagc
    10681 agcttagcgg agccaggctg gagctgctga aaagtcagat caatccccat ttcctcttta
    10741 atactctgaa tatgatcgca tgcatggcaa agctggagga ggcggttacg acagagcgga
    10801 tgatcagcag catgggcagc ctgttccggt ataacttaaa gacctcagag cagatcgtaa
    10861 cccttgccag ggaactgaag gtggtgcagg attatatgta tattcagcag atgcgttttg
    10921 gaagccgtat tctctacagc tgtgatctta aggttgatgc agagcaggct atgattccgg
    10981 cctttacgct gcagcctgtg gtggaaaatg ccatggttca tggtttgtca aagaaggaac
    11041 agggcggaag agttcacgta aggatttggg aacagggaaa caggcttgtt atttctgtag
    11101 ccgatacggg gctcggaatg tctgaggagc gtctggcaga ggtcacggag gccatgaagg
    11161 agcgccggac ctccaggatt ggaataggac tgggaaatat ttacaagcgt atccacatga
    11221 tgtacaaaca gggagaattc cggatcgcca gcatagaagg acggggcacg gtgattcaga
    11281 tgttcattcc tcaggaaaag cataacaggg gggataaata atgtaccgaa tgttgatagc
    11341 ggatgatgaa atgatagagc gggctgtact gtataaaatg ctcaataaaa atctggaagg
    11401 ccagtgtgag atctatcagg cagaaaacgg cagacaggca ctggagctgt atgaaaagga
    11461 gaagatccag attgctgttt tagatataga gatgccgggg atcaacggga tccaggcagc
    11521 agagaagatc agggaaaaag ataaggattg tgtgatcatt ttccttacag cttttgatga
    11581 attttcctat gcaaagaaag ctatttctgt aagggccatt gattatgtgc ttaagccata
    11641 tgatgaaaat gagcttatgc ttgtgatcga ggaggccatg cgcatggtag aagagcaggg
    11701 gcagaggaag gagatggaac agttctttca aaatgaatcg gaggaagctg attttgaaga
    11761 agatccggtt cagccggagg atgataatga tcacatgagc cgtgtagggg ccatgatccg
    11821 ctcgtttatt gaagaaaatt atatgtatga catttccatg caggagctgg cacggaggat
    11881 gaactactca gagccctatt tctgcaagct ttttaaacag tactttgacc agaattttac
    11941 tgcttatctc accgagtacc gcattgctga ggcaaaaaag cttttggagc agcccacttt
    12001 taatataaag gatgtgggaa aagcggtagg ctacagtgat tccaattact ttacaaaggt
    12061 atttaagagg atcaccggac agagtccgac ggagtatcgg aatgggtgat aaggaaaaat
    12121 ataaagaaaa tataaagaaa gttaaaatta tactacgatt cgcaaaaatt taccatgttc
    12181 attgtgcgct tttccttata taatgaaatc aacataaata aagcgttaag atatatatca
    12241 aaaacgtgaa tgatgtatta agaggaggat gtgtaacatg aagaaaagat ggttaagcac
    12301 agcactttgc gcagcaatgg ctgcaggtac tctggcaggc tgcggtctga agacaccaga
    12361 gactacgaca gctgctccgg ctgcaactga ggctgcaaag gaggagacca aggcagaaga
    12421 gaagactgag gcggcagagg gtgagaccaa ggcagaggca gcagcttccg gcaagtatga
    12481 cactgcaaat atgcctgagg taactctggt atacgcagag gttaacccac tggatacgat
    12541 cgttggccag atggctaccg atttcaagga aaaggttgaa gagctttccg atggaaagat
    12601 gacaatcgac gtacaggcca gcggcgttct gggttctgag aatgacgttc tggataccat
    12661 gttaggcggc ggcggtacca ttgatatgtc ccgtatttcc gcattcgcat taaacggcta
    12721 cggcgcaaag aagtctctgc ttctttccat tccttataca tttgctgacc gtgagcattt
    12781 ctggaacttc gcaaccagcg atctggctga tgagttctta gcagagccgg ttgagcaggg
    12841 cctgggcgta agaggtctgt tctacggtga ggaaggcttc cgtcacttct tctttaagga
    12901 agaggctaac acacttgaag acttaaaggg cttaaagatc cgtgtttcca atgatcctgt
    12961 tatgacaggt atggtagccg gactgggcgc taacgcaact gttgtttcca tgaacgagct
    13021 gtactccgca ctgcagacag gtgttgttga cgctgctgag cagccgatcg caaactatgc
    13081 ttccaatgca ttccctgagg ttgcacctta cctgatgctg gatggacata ctctgggcgc
    13141 tgttcaggtt gttatcacag acgaggcatg gaacggactg accgaggatc agcaggctat
    13201 ccttatggag gcaggcgagt acgcttctgc taacaaccgt gcaaactccg agaacaagga
    13261 gaacgaggtt ttagagcagc ttaaggctga cggcgtaacc gttgttgagg ttgaggataa
    13321 ggctccatgg aaagaggctt gtaaagatgt aatcgagtcc aacacttccg atcaggctga
    13381 actgtatcag cagctccttg atatggagta atcttgcagt aaatttgtaa ctgacaggac
    13441 acagagagtt ttggatattt tctgtgtcct gttttggtat cacggaacct gttaaaatgt
    13501 tgttaacaga catccgaata cacaataaag aaaaggggga aaatctgaaa tgccagagat
    13561 tttcaacaaa atcgacaaga tcaaaccggc gtatgacctg gtatacaagg ctgtaatgct
    13621 gatctgcaag ctgcttctgg ttgcggatat tgtgatcaca tccatgtctg ttgtcggccg
    13681 ttacgtttcc tttattcccg acccggcctg gagtgaagaa gtagttctta cctgtatgtc
    13741 ctatatggca gtgctttctg cagcactggc gatccgcagg ggcgctcata tccgtatgac
    13801 cgcatttgac aattacttac cgaaaaaggt cgtaactttc cttgatatcc ttgctgatgt
    13861 tgccgtactg gtactggctg tgatcatgat tacagtgggc tggaaatatg caaccggaat
    13921 tggttccaag ggtacttatg taagtatgcc aaaggtatcc agattctgga tgtacttccc
    13981 ggttccttta gccggagtgg ctatggttgt gtttgagatt gaggctcttt acaatcatgt
    14041 gaaaaagatc tttgtaaagg aggaagcaaa ataatggatg cgaatacatt tgcaatttta
    14101 gttttacttg gaagcttttt cctgatgatc ttcctgcgtt tccccatcgc ttatgcggtt
    14161 gctctttctt ccgtattctg tctggtagct cagggacata acctgactac gatctgccag
    14221 cagatggtaa agggaatcag ttccttcagc ctgatggctg ttccgttctt tatcaccatg
    14281 ggatgcctta tgggctccgg cggtatttcc gaaaagctga tcgctctggc aaatgcctgc
    14341 gtgggctgga tgcgcggcgg tatggcaatg gtaaacatcg ttgcttccta tttcttcggc
    14401 ggtatttccg gttctgcatc tgcagataca gcttccttag gttccatcct gatccctatg
    14461 atggttgacc agggctatga cggagatttc tccactgcag ttacgattac atcttcctgt
    14521 gagggacttt tagtacctcc aagccacaac atggttatct acgctaccac agccggcggt
    14581 atttccgtag gaagcctgtt cttagcagga tatattccgg gcgctctgtt agctgcttcc
    14641 ctgatgatcg gctcttacat tatttctgta aagcgcaatt atccaaaggg cgacaagttc
    14701 agcatgaagg tgctgttaaa gcagttcagc gtttccttct gggctctggc agccgttctg
    14761 atcgttgtag taggcgtagt aggcggtgtg ttcacagcta ctgagtccgc agcgatcgct
    14821 gttatctaca gtcttcttgt aagtgtattt atctacaagg gacttgactg gaagggcgta
    14881 tggagagttt taggcgactg catcgacaca ctgtctatcg tactgatcct gatcgcaacc
    14941 tccagtattt tcggttactg cctgacacag ctccatgtgc cggatctggc agcaactgct
    15001 attacaaact ttaccaacaa cccaatcgta ctggcactgc tgttaaacct gatccttctg
    15061 gttttaggct gtatcatgga tatggctccg atcatcctga tcgcaactcc tatccttctg
    15121 ccgatcgcaa cctctatcgg tatcgatccc attcagttcg gtatcatggt agtattaaac
    15181 tgcggtatcg gccttctgac tcctccggtt ggttccgttc ttttcatcgg ttccgccatt
    15241 gcaaagctgc caatggagaa ggttgtaaaa gctacccttc cattctacct gtgcatgatc
    15301 attacgctgc ttctgatcac atttgtaccg gcaatcagcc tgtggctgcc tcaggtattg
    15361 gcataacagc agaaatcatt tataattaac aatcatgaag ccgttctgat gctttatcag
    15421 gacggcttta aaaatcactt cggaggaagg cttatgtatc aatataccta tcgtctcagc
    15481 aatggagaat actggcgtct gtctatgtac tatacgtatg gatctatggt aggcgtgtgc
    15541 aatctgatat ttacagcggc tttatttatc cttacctatg taaaatgggg aggagcagga
    15601 tggggctacc gggccatgct gatcttaggc tgcagtctgt ttaccgtgct tcagcccctg
    15661 gcgatctgga gacgttccaa aaagattgct tcagcgatta aagaggatac tcatatcatg
    15721 attgacggca aggggatcca tgtgactgtg ggaacggctc atacggatat tgtatgggac
    15781 aagatccgcc gtatttcaaa gaaaccggat atgctggtga tcttttccga tacgacccat
    15841 ggatatgtgc ttacggaccg ggtgctggga ggagaccgtg aagcggtcta ttcgtttatc
    15901 actgcccgta tgaatcatgg aaaaaagtaa ggaggaagca gtgaaaggga agagaaacat
    15961 cctggctgcc ggcctgatcc tggcgttgtc cgcctcggta tgggggtgcc aaagcagaga
    16021 gaagggggct ggggaagaaa atgtaccaga gtttgtgctg acctatgcgg aaaatcaggc
    16081 agaggactat cctactactc agggagctta cagatttgca gaactggtaa agcagagaac
    16141 cggaggaaag gtggaaatcc tgatcaatgc cgaggggatt ctgggggatg agagaacggt
    16201 gatcgagcag cttcagttcg gcggcgttga ttttgcccgt gtgtctctgt cgcctttatc
    16261 ggaatttgtg ccggaattga atgtccttca gcttccctat ctgtatacag gtgcggagca
    16321 tatgtggaag gtgctggaag gggaaatcgg ggacagcttt ttaggttcct ttgagggctc
    16381 cagtctggta gctctttcct ggtatgacgc aggagccaga aatttttaca attcggtccg
    16441 tcccatagag aagctggagg atatgaaggg gcttaccatc cgtgttcagg agtcggagct
    16501 tatgagcgac agcatccgcg tgctgggagc tgtgccgaag cagatggctt acggggaagt
    16561 ttattccggt cttcagaccg gggaaatcga cggagcggaa aataactggc cctcttatga
    16621 gtccacaagg cattatgagg tagccaggta ttacaccatc gatgagcata cccgagtgcc
    16681 ggagctgcag ctggcggccc agtccacctg ggataagctg cctgaggagt acaggatcat
    16741 tattacccag tgtgccaaag aatccgcaag gtatgagcgt tctctgtgga aggagcgcga
    16801 agcacagtct gagcagaata tccgtgaagc aggctgtatc ataacagagc tttccaaaga
    16861 agaaaagtcc cgctttcagg ctgcggttat gcctatgtac ggaaaatact gcagcgatta
    16921 tgtggatatg atcgaccgga ttgtggcaga ggggagataa ggaaaaatca cgtccggttc
    16981 atccttgtgg aatgacggta aggtatgcta taatagcccc ataaccggat ggctcttatg
    17041 gatccataca ggcacagaca aaggagaagg gacggtggac gttatgaata aagtaattac
    17101 agtaagccgt gaatttggaa gcggtggacg cgagctggga gtaaagctgg cagatgcgct
    17161 ggggattcct ttttatgata aggaactgat ttccatggct gccgacgata tcaatattgc
    17221 ggaagaagca tttcgcaggt atgacgagca tattgtggtg gaagatcccc tggatcggaa
    17281 tttttatcat gcattttctg acatttacca ggttcccatg tcggatcaga ttttcgtggc
    17341 gcagtcgaat gtgatccgcc gtctggcatc ccatggcccc tgtattattg tcggccggtg
    17401 tgctgatatg gtattggagg acagcatcaa tatttttgtt tatgcaaaga tgaaaaacag
    17461 gatccgccgt atgatggagc tggagccggg atcggatgaa aaggaaatgg agcgccggat
    17521 ccgtgaggta gaccggaagc ggaaggatta ttatcagtat tatactggaa atacctgggg
    17581 aaaggcgcag aattatcatc tgtgcgtaga cagcggactc gtaggagtgg acggctgtct
    17641 cagagcagtg acggcatatc tgggagagtt gtgccgggat ctgtcttaaa aaaagaaaat
    17701 atatgtttta tgaatattat ttttccaaag gctcatacta ggagtgtaca aaggaagtac
    17761 aaatcaaaaa acaggtgagt ccatggaaaa gcccggtaaa aaactgaaga aatttttaat
    17821 cataacagga accacaggat tggtctatgc aggtttaaag tacctgctgc ctcttgtggc
    17881 tccttttttc attggctata cgctggcctt gatcctgcgc ccttcggtcc gttttatccg
    17941 gtatcgtttt cagttttctg catttggaaa aacatggaaa attccctctg gattgatcgg
    18001 agcagcagaa atagggatta tatttggcgg tctggggatt tgggtctggc agggaatcct
    18061 gcgcctgtgc cgggaactgc agcttctggc agtccgtctg cccctgtgga tcatggattt
    18121 tgacagccgg atggcggaat actgtatgaa aatggaacgg gcagcaggct taaagtccgg
    18181 ctgtctgttt gaaacagcag ctgaaatgac agccacattg ggggagaagg cgaaagaaag
    18241 cctgatgcct tttcttatga caaattccct gcctgcagcg gcttttttta tacgtctgac
    18301 tctcgtttgc ctggtttcct ttctggcggc cgtcttgagt cttcaggaga tggaagagct
    18361 tcggcacagg cgggatcagt ccttgtttcg cagagagttt aatcttatcg ggagccgcct
    18421 ggtacagacc gggaaggcat ggtttaaaag ccagggcatc atccttcttt taacctgtgg
    18481 tttgagtata ggagccatga tctggatggg aaatccatat gctgttttgg caggcacagc
    18541 catagctctt ctggacgccc tgcctctttt gggagcaggg gctgtccttc tgccctgggc
    18601 ttttttgtgc gtgctgggag gacagtggaa ggatgctttg atcctttcgg ctctctatct
    18661 ggcctgttac tttgcgcgcc agtatctgga agcgaagtat atgggcagtg aggttggctt
    18721 atctcctctg gaaacactgg cggcagtgta tgtgggaatg gaattatttg gttttttggg
    18781 atttatttta gggcctctgg gactgctttt gatcgaggat ctgacagaga tatgggaaga
    18841 aggagcagac agagggaaac ttacttgaca ggcagaagct ttggctgata gactggtatt
    18901 agaaatgatg ggagaggaca tgaaaatgtt tgatgtggag caggtaaaag ccttaaatga
    18961 tctggagaga atggtctatg aatatgttgt aaaagatcct gaaaaggtga aatatatgag
    19021 gattcgggag ctggctgatg aggtacatgt ttcaacgtct accattctgc ggttttgtaa
    19081 aaaaatggga tgtagcggct attcagaatt taagctgcag cttcgggatt atcagaccag
    19141 gaaggatcag gcaaacgtaa gggatgatat ttcagaagtc gttgactttc ttaaaaaaac
    19201 cgcagcgcag gagtttgaag ataagctgga aatgctggca gaggttattt ttcatgcaaa
    19261 ccatgttttt ctggtaggca gcggtatgtc ggggatcgtg gcaaaatatg gagcgcggta
    19321 tttttcaagt atgggtaaat tctgcctgta tattgatgaa ccgcactatc ctacagagag
    19381 cagtttttta aaggacagtc ttgtgatcgt attttctgta tcgggggaat ccggagatac
    19441 catccggcat gttatgcggt ttaaggagca gaactgccgg atcctcagca tcacgaatac
    19501 ggaaaactgc accatagcaa agctgtctga tcataatatt gcatattatg tcagctatat
    19561 gcggctgggt gattatgatg ttacaaccca gatcccggcg gtgagtattg tggaacgtct
    19621 gggcaagaag ctgtaccgct atacctctaa agggccagga gctatttgat aaaacatcct
    19681 gttacatata aaaaacaagt gattaaaggt gtcacttgtt tttttaggcc ctgaaagcga
    19741 tgacagtaga tgattccctt cgtataatgt atctatcaaa atccttaagg gggaaaagaa
    19801 aatggcaaaa gattatacgg ttatttccag aaaaatcgta gagcagattg gtgggatcga
    19861 caacattgca aacgttactc actgtatgac ccgtctccgc tttgtgttaa aggatgaaac
    19921 actggcagat gacgaggcag taaaagcaat tgacggtgtt atgggtctgg taaaacaggg
    19981 aggacagtac cagatcatca tcggcaacca tgtaggaacc gcttatcagg aggttttaaa
    20041 actgggaaat tttggcgagc agagtgaaat tgccaaaggt gagaagaaag aacctctgac
    20101 actgaaaaaa gtgggaaata acattctgga tgcgattgtg ggaaccatgt ccccgctgat
    20161 tccggctatc atcggcggct ccatggtaaa gcttctggtt atgctccttg gaatggcagg
    20221 tatcctgtct gctgattcag aaacctataa gctgatcaat accattggcg acggagcatt
    20281 ctacttcctt ccgatgctgg tagcagcgtc cgcagcaaag aaatttaaga cggatatgtt
    20341 tctggcaatt tccattgcag gagttctgat ccatccggat ttcagaagca tgatggaggc
    20401 agttacagta ggcgatacag cggctcattt tatgggaatc cccattgctt ccgtaaagta
    20461 tacctatacc gttatcccgt caatctgtat gacatggatc ctgtcctata ttgagcgggg
    20521 cgttgataag atcactccgg ctgtgacaaa gaacttctta aagcccatgc tgattcttct
    20581 gattgcggct cccattgcta ttttaatcat tgggccggca ggtatatatg taggagaagg
    20641 catttcctca ttcgttttcg cagttcagga caagcttggc tttgtggcag taggaatcat
    20701 gggcgcgatc tggccgctcc ttgtaattac cggtatgcac cgtgtattta cgccaactat
    20761 tttacagact atttccgaga cgggaatgga gggtacagta atgccctctg agatcggagc
    20821 aaatctttca ctgggcggcg tatctcttgc agttgcatta aaaacaaaga acagtgagct
    20881 tcgacagacg gctcttgcgg cagcatcttc tgctctgatc gcaggaacta cagagcctgc
    20941 tctctatggc gttgcagtgc gcttaaagcg tcctcttctg gcttctctga tcacaggctt
    21001 tgtttgtggc tgtctggccg gaatcggagg gcttgccagc cgttccatgg tatcgcccag
    21061 cgttctcacc ggagtacagt ttatcgatcc ggcgaatccg ggaagaagta ttgcgtggat
    21121 tgcaggcatc accattttat ccattgtcct gtcctttgtg ctgacactgg tgatcggttt
    21181 tgaggatatt ccagaagagg agaaataaca ttgagtgatt tcagagtacc ggagaatttt
    21241 ttatggggag gagccatttc tgccaatcag gcagaaggag cttatcggga aggaggccgt
    21301 gggatcagca atatcgacct gatcccccac ggttccagtc gtatggatgt gaagctggga
    21361 aaggtttctg atcctgagct gcagacagat gccttttatc ccagccatga ggcgattgat
    21421 ttttatcatc attataaaga ggatctggcc ttaatggggg aattgggact taaggtattc
    21481 cgcacctcta tttcctggtc cagactgttt cctgagggag atgaggcatt ccccaatccg
    21541 gaaggaatcg ctttttataa agacctgttt aaggaatgcc gcagatatgg gatggagcct
    21601 ctggttacgc tgtgccattt tgatgtgccg tatggcctga tccggaaata tggctcctgg
    21661 agaagcagag aggtgatcgg ctgctttgta cggtatgcaa aaacatgctt cagggaatta
    21721 aagggactgg taaaatactg gcttacattt aatgaaatca atattgtgct gcacagtccg
    21781 ttttcagggg caggaattgc ctttgcagag ggagaaaaca gaaatcaggt tatttatcag
    21841 gcggctcacc atatgcttgt agcaagcgct cttgtcacaa aagcagccca tgaaacagat
    21901 ccagaaaata aagtgggctg tatgctggcc ggaggaagct tttatccgta ttcctgtaat
    21961 ccggaggatg tatgggagtc tgtaaaaaaa gaacaggaaa atctgttttt cattgatgtg
    22021 caggttcgcg gacattatcc cagctatgcc aaaaagcttt tcagggaaaa gggtatctgt
    22081 ctgaatatgg agccggaaga ttcggatatc ttaaaaaata cagttgattt tatgtccttc
    22141 agctattatt ccagccgctg tgtggctgcg gatatggcgg ataagacgtc aaatgaggga
    22201 aatgtggtga aatccgtgaa aaacccctgg ctgaaaacaa gccagtgggg atgggccatt
    22261 gaccctcttg gtcttcggat cacaatgaat cagctttatg accgctatca gaaaccgctt
    22321 tttattgtag aaaatggtct tggcgcaaga gatgtgctca caccggaagg aacggttgag
    22381 gatgattacc ggatcgacta tctgaaaaag cacattgagg cgatgaagct ggcaatggaa
    22441 gacggagtag agcttatggg ctatattgtc tggagctgta ttgacctggt gtctgcatct
    22501 acaggggaga tgagcaaacg atatgggttt gtttatgtag acagagatga tgaaggaaaa
    22561 ggaagtctta agagacagaa aaagaaatcc ttttactggt atcaggatgt aatccgaagc
    22621 aatggggagt atttataaac acttagaaaa tacaaaaaat aaaaggtacc acaaaagttt
    22681 ttgtataaag tacctataaa agagcttggt ttttcgatac tttttaggat agacaaataa
    22741 aatgggaatg cttataatga aagtaacttc ataaaagcag attttttatg tatatcctgg
    22801 agattggtcc agagtttcta ccaactgccg taaatagttg actacatagt ttcatggggc
    22861 tggttttggg cggccgccat gcagctgtgc gcggtctttg gggcagatat gagcagattt
    22921 aggttccgcg ttggcggaac cttttttagt tcataggaaa gagcatccta tgaaattcag
    22981 tgggttacac aagcagagat tatctgtaga gaaaggaggg atgaggcaga acgcacgcag
    23041 actggctgct gatgaagaaa aatgtgaaag agaggacata acattggaaa aaagggaagg
    23101 ttttttcgaa cggttttttg ggatttcaca aagcggctct aatatgcgca cagaggtttt
    23161 ggcagggatc accaccttta tcacggtggc gtatatcctg atcctgaatc ctcagatcct
    23221 ggctgatcca tttgtcatcg ggggagacat ggatatggcc tctaaaatct ccaacggcgt
    23281 atttattgga acctgcatcg gcgcttttat tggtacgatt ctgtgtgcgg tatatgccaa
    23341 ggtgccttta gctcaggctc ccggtatggg gctgaatgca ttttttgcct atacggttgt
    23401 gctgggaatg gggtatactt atcatcagac attgacaatt gtatttattt caggcgtact
    23461 gtttatcgta atcacggcag tgggactgcg ccaggcgatt atccgggcca taccggagca
    23521 gattaaaatg gcgatcacgc cgggaatcgg cctgtttatc accattgtgg gcttaaagaa
    23581 tgcggggctg gttgtaagca atccctctac gcttgtaagc atggttgatt ttgcccagtg
    23641 gagaaatggc gtggatacac agctgatctg cggcgctctg gtggctttga tcggactgat
    23701 tgtcatagga gttcttcaca caaagaatgt aaagggttcg attctctggg gaattgtagc
    23761 agccacagtg gtaggaatcc ctctgggagt gacaaagctg tctgcctttg acctggatct
    23821 ggctgcaaaa ttcagagatt ttggtgaggt ttctctgttt aaaatggatg tggcaggcct
    23881 gtttgaggga aagagtgtta cagatgccat ttttactatt attatgctgg tactcagttt
    23941 ttcactggta aatatgtttg actcaatggg aacacttatg ggagcaggac gccagtccgg
    24001 tctgatcgat gagaacggag aagtgattca tatgcaggaa gcactgatgg cggacgcgat
    24061 ttccacagca gcaggagcat tagtgggtac ttctaccgta acgactgtag tggaatccag
    24121 cgcaggcatt gcagcaggag gacggactgg aatgaccagc ctggtaacag cagctctttt
    24181 ccttggagcc attatttttg ccccggttgt aagcattgtg ccggcagcgg caactgcacc
    24241 ggctctgatc tttgtgggcg ttctgatgct gagcaatatc aaggatgttg atttttcgga
    24301 tatgacaaac ggacttccgg ctttctgtac cgttgtattt atgcctttta cctattcaat
    24361 tgcaaacggg attgcaatgg ggcttattac atactgcctt ctgaagatcc ttgcaagaag
    24421 accgaaggag gtagaagtac tgacaggact gatcgctgtg gtatttgtgt tccgctatgc
    24481 atttatgacc cttggataaa agggaattgt aaggaggatg gaatctatgg aacagaaaac
    24541 atttgcactg aagggaaata tcatttatac aaaggagcga accctatttg aagtgatgga
    24601 acagggctgg ctggtatgtg aagaaggccg ggttcgggat gtgtattcgg tgcttccgga
    24661 ggaattaaag ggcatacccg tggaggatat gggggatcgc ctgattatcc ccggactgac
    24721 ggatctccat atccatgctc ctcagtatac attccgcagc cttggaatgg acatggagct
    24781 gttggagtgg ctggaaacaa atacatttcc ggaggagtcc aaatataagg atgcggagta
    24841 tgcccaaaag gcctatggaa tgtttgcaga gcagttgaaa aagagcgcta ccgtgcgggc
    24901 ctgcattttc gggaccattc acaatgaggc cacgctgatt ctgatggatc ttttagagga
    24961 ggccgggctt cacaccatgg ttggaaaggt aaatatggac cgcaacagcc cggattatct
    25021 atgtgagcca tcagctgagg cttccgctca ggctaccagg gagtggctga aggccgtagc
    25081 tgaccggaag tataagcata ccagaccgat tctgactccg cgttttactc ccagctgtac
    25141 agatgagctg atggagcttt taaaggagat tcagacggaa acccatcttc cgatgcagtc
    25201 tcatctatca gaaaatccgg gggaaattgc ctgggtgaag gagctttgtc cctggtctga
    25261 attttatggc gatgcttatg ataagtttgg actgttcggt ggagactgcc ccactgtaat
    25321 ggctcactgt gtttattccg gaaaagagga aatggccctt atgaaggaga aaggcgtatt
    25381 tgtggcccat tgtccggaat ccaataccaa cctttcttcc ggcgtcgcac cggtgcggcg
    25441 gtatctggag gagggccttc atgtgggtct tggaagcgac gtggcaggag gttctacaga
    25501 aaatctcttt accgccatgt ctcaggccat ccaggcatcc aaattaagat ggaggctgca
    25561 ggatgactcc ttaaagcctc ttaccgcaga ggaggtcttt tatatggctt ccaagggcgg
    25621 cggagcgttc tttggaaaga caggaagctt tgagccggga tatgaatttg atgcggttat
    25681 cattgacgac agccgcctga tccatcccca accgctgacg gtgaagcagc gtctggagcg
    25741 agtgatctat ctgggcgacg aacgggaggt atacggaaaa tatgcttgcg gtgaaaagat
    25801 tctgtaacta ttctgagaaa ataaaggtga aatgatgaga ggaaaatgta ctcccggaat
    25861 ccctgtatgg ggctttcggg agcattttct gttatacagg aggaagacag ggtggaatgg
    25921 aaggatggaa aatgcaggtg ctgctgggcc aatccaaaga atgagcggta tatccgctac
    25981 catgatgagg aatggggacg ctcggttcat gatgacagaa agctgtttga aatgctggtt
    26041 ttggagtgtt ttcaggcagg gctttcctgg gaatgcattt taaataagag agaggccttc
    26101 aaacaggcat ttgatgagtt tgagctggaa actgtctgcg ggtatggaga ggagaagctt
    26161 caggaactga aagaaaatgc gggaattgtg cgtaattctc tgaaaataat tgccgctgtc
    26221 accaatgccc gtatatttag aaagattcag gcagagtacg gaagctttga ctgttatctg
    26281 tggcactgga cagagggaaa ggtaatctgc gagacaggca agacaaactc tgctttgtct
    26341 gaccggattt cagaagatct taaaaagcgc ggaatgaagt ttgtgggaac tacagttgta
    26401 tatgcctatc tgcaggcggt tggagttgta tggggacatg aagaaaactg ttttctgtca
    26461 ctgcatcagg gaaagaatat gcagaaaaag taacagtttc ataacggttt cttacatttt
    26521 tctgtcttgt ttacatttcc tatacgtttt cttacttctt ttaaaaatca ttgatttaat
    26581 cgaaaaaaat agctataatc tgctcataaa aaacagataa aataaacaat gcatataaaa
    26641 ggaggaggtt tcctatatga gaaaacgtaa tttaaccggt ttattatttg cagcagcatt
    26701 tgtgatggca tgaacttggt caacggcatg g
//
LOCUS       DBMDUH010000016        26452 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_1288_length_26452_cov_7.873212, whole genome shotgun sequence.
ACCESSION   DBMDUH010000016 DBMDUH010000000
VERSION     DBMDUH010000016.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 26452)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 26452)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26452
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1288_length_26452_cov_7.873212"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="ACZPCG_02790"
     CDS             complement(<1..712)
                     /locus_tag="ACZPCG_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HIR1284030.1"
                     /translation="MKRKMTAMCFAAVLLSAGTVNLTAWAEGWAEAEGNWVYYDSNGN
                     KVYNDWKKGADGQWRYLDGNGTMAVNSWVDGEYYVDSNGIMVTEKWLHVPEDDGEDEW
                     YYFSASGKKVEDTWKKISDKWYHFDSNGAMERGWILDDTYYTGDDGVMRTGWQKLIPP
                     DEYDEEINKVVPGYDTGNVEYGEDGCYWFYFGTNGKKYVPSDDNSGDDYGTRKIDGTY
                     YCFDEDGILQIGWKDVRDN"
     gene            complement(971..2443)
                     /locus_tag="ACZPCG_02795"
     CDS             complement(971..2443)
                     /locus_tag="ACZPCG_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HIR1284031.1"
                     /translation="MKYLQKLGKALMLPVACLPICGILMGIGYYLCPSTMQGGEVEGI
                     LQQIGFLLVKAGGALIDNMAILFVIGVGVGLSEDHDGTGGLAALASWLMITSLLSTGV
                     VTTLMPSIAEDQQTTLAFSKIANPFIGILSGIIGSTCYNKFKGTQLPDWLSFFSGKRC
                     VTIIAGVVAIITSVILLFVWPVLFGGLVAIGNGIAGMGAIGAGIYAFLNRLLIPTGLH
                     HALNNVFWFDTIGLGDLSHFWAGETSADVTWSLGMYMSGFFPCMMFGIPGAALAMVQT
                     AKSTKKKVAIGLVASAALSAFVCGVTEPFEFGFMFLAPGLYVVYALLYGIFTTVTTIL
                     GFRAGFSFSAGATDLFFSASLPAAQKTWMILPLGIAAFLVFYVVFRFAIVKFDLKTPG
                     REDDDDLEAEKSAVLANNNYTEVASVILEGLGGKENVKSLENCITRLRLEIKDQSVVD
                     EKKIKSAGVAGVIRPGKNSIQVIVGTKVQFVADEMKKMLK"
     gene            2888..3748
                     /locus_tag="ACZPCG_02800"
     CDS             2888..3748
                     /locus_tag="ACZPCG_02800"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR1284032.1"
                     /translation="MWLADGWNDYEVIDCSKGEKLERWGDYLLVRPDPQVIWDTPKTH
                     RGWKKMNGHYHRSSKGGGEWEFFDLPQQWTINYKELTFNLKPFSFKHTGLFPEQAANW
                     DWFGEKIRRAGRPVKVLNLFAYTGGATLAAAKAGASVTHVDASKGMVTWAKENAHSSD
                     LADAPIRWIVDDCVKFVEREIRRGNHYDAIIMDPPSYGRGPKGEIWKIEDAIHPLVKL
                     CVQLLSEDPLFFLINSYTTGLAPSVLSYMMGVEIVPKFGGTVKADEVGLPVSQNGLTL
                     PCGASGRWEK"
     assembly_gap    4053..4152
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4166..5056)
                     /locus_tag="ACZPCG_02805"
     CDS             complement(4166..5056)
                     /locus_tag="ACZPCG_02805"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HIR1284033.1"
                     /translation="MNRARVDIHCDQVYSMGITGRGIGVAVLDTGIFLHEDLKDRVKG
                     FADFVRRKDRPYDDNGHGTHVAAMIGGSGASMEGKYRGVAPGCSIISVKVLDHRGNGY
                     ASDVLSGLRWIRSHKEFLGIRIVNISVGSLSKRDMTENSALVKGVNAAWDDGLVVVVA
                     AGNHGPGPMTVTTPGISRKVITVGCSDDHKEVEVMGNRMVDYSGRGPTASCICKPDIV
                     APGCGIVSCGNEAGAYFAKSGTSMSTPLVSGAIALLLEKYPDMTNRDVKLRLRERAID
                     LGLPRNQQGWGALDVQKLLQ"
     gene            complement(5302..5919)
                     /locus_tag="ACZPCG_02810"
     CDS             complement(5302..5919)
                     /locus_tag="ACZPCG_02810"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1284034.1"
                     /translation="MIKNIVFDMGKVLVEYEGDRVCRHFSEDEALIRKVSTAVFVSPE
                     WLMLDMGIISEEEGLSQILTRLDTEEEKELARKSFAHWHEYNMYHKEGMEELVRELKE
                     EGYRIYLCSNASVRLLTCYHQVIPAIDCFDGIFFSAEVKCMKPQKEMYEHFFERFGLK
                     PEECFFIDDLERNIQGAKACGMDGYCFADGDRERLKIRLASFKKG"
     gene            complement(5993..6271)
                     /gene="yajC"
                     /locus_tag="ACZPCG_02815"
     CDS             complement(5993..6271)
                     /gene="yajC"
                     /locus_tag="ACZPCG_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006778824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HIR1284035.1"
                     /translation="MAGMGGIILYIIFLGALVYFMAIRPQKKERQKQQELLASVAVGD
                     TILTSSGFYGVIIDMTDDTVIVEFGSNKNCRIPMQKVAIVQVEKPETK"
     gene            complement(6323..7459)
                     /gene="tgt"
                     /locus_tag="ACZPCG_02820"
     CDS             complement(6323..7459)
                     /gene="tgt"
                     /locus_tag="ACZPCG_02820"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HIR1284036.1"
                     /translation="MKYQVITKDGRAKRAVMETVHGTIQTPVFMNVGTVGAIKGAVST
                     DDLRQIGTQVELSNTYHLHVRTGDKLIREFGGLHRFMNWDKPILTDSGGFQVFSLTDL
                     RKIKEEGVYFSSHIDGHKIFMGPEESMQIQSNLGSTIAMAFDECPPSHADYDYIRKSV
                     DRTTRWLKRCKSEMTRLNSLPDTVNREQLLFGINQGGVIDEIRIDHAKTIREMDLDGY
                     AIGGLAVGETHEEMYHILDVTVPHLPEEKPVYLMGVGTPANILEAVDRGVDFFDCVYP
                     SRNGRHGHVYTNHGKLNLFNQKYELDHRPIEEGCQCPACRSYSRAYIRHLLKAKEMLG
                     MRLCTLHNLYFYNKMMEEIREAIEEHRYSEYKAAKLAGMAGQKD"
     gene            complement(7463..8860)
                     /gene="scfB"
                     /locus_tag="ACZPCG_02825"
     CDS             complement(7463..8860)
                     /gene="scfB"
                     /locus_tag="ACZPCG_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007707732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HIR1284037.1"
                     /translation="MIHQYINNGYHIVLDVNSGSVHVVDPVVYDAIKAVAEQIPELEK
                     PQALPAEVTDRVHEALDGTYSEKEVTEALEDIQQLIDAEQLLTQDIYRNFVMDFKKRK
                     TVVKALCLHIAHDCNLACRYCFADEGEYHGRRALMSFEVGKKALDFLIANSGNRRNLE
                     VDFFGGEPLLNWKVVKQLVEYGRSQEEAHNKRFRFTLTTNGVLLNDEIMEFCNREMSN
                     VVLSLDGRPEVNDKMRPFRNGSGSYDLIVPKFQKFAESRGQMDYYVRGTFTRNNLDFS
                     KDVLHYADLGFKQMSMEPVVAEPEMDYAIREEDIPQILEEYDKLAVEYIKRKKEGRGF
                     NFFHFMIDLNAGPCVAKRMAGCGSGTEYLAVTPWGDLYPCHQFVGNEEFLLGNVDTGI
                     VNEEIRNEFKLCNVYAKESCKDCFARFYCSGGCSANSYNFSGSINGAYEIGCEMQKKR
                     IECAIMIKAALADEE"
     gene            complement(8975..9121)
                     /gene="scfA"
                     /locus_tag="ACZPCG_02830"
     CDS             complement(8975..9121)
                     /gene="scfA"
                     /locus_tag="ACZPCG_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005540245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HIR1284038.1"
                     /translation="MKHVKTLNTNTLKDTMKKGGCGECQTSCQSACKTSCTVGNQSCE
                     NADR"
     gene            9301..9663
                     /locus_tag="ACZPCG_02835"
     CDS             9301..9663
                     /locus_tag="ACZPCG_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HIR1284039.1"
                     /translation="MENGIVKPMLRSLLISYVLSGLLLGALAFALYKLRLKEGQVNLM
                     VYLVYLAACLAGGAAAGKRLRQRRFFWGLLSGLFYFLILFAVSWAMNPWSALDTERSV
                     TVMAVCALGGMLGGMFSA"
     gene            complement(9665..10141)
                     /locus_tag="ACZPCG_02840"
     CDS             complement(9665..10141)
                     /locus_tag="ACZPCG_02840"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HIR1284040.1"
                     /translation="MKYLIWLVGGIAALDQGMKSEIEQETDGEFPRDLPGAKGRIRIH
                     RNHNYGFPFGFLKEKPQLVTAVPLAVSSAVAGALSMLLVRKGCGIQKVGLSMVLGGAL
                     SNLYDRFKRGYVVDYFSFSLPGIKKVVFNLGDLFVFLGTALFLLGEMGEEFKKKVD"
     gene            10504..12252
                     /locus_tag="ACZPCG_02845"
     CDS             10504..12252
                     /locus_tag="ACZPCG_02845"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HIR1284041.1"
                     /translation="MPDCPFNPAAENIADLIYRFGSLQSERWNNTSSLCADYVSSDYI
                     ILHMPLTPDAVSMRVHSYYMIPSLFSPLDYNAMEASGILSAFNSPALNNQGKGVLIGL
                     VDTGIDYRSPLFQNPDGTTRIAGIWDQSVPIEEDVLPAGVPDYYPMGGASYGTEYTRE
                     QINKALASDDPLSLVSTQDTIGHGTFLAGLAAGSSIPQEDFTGAAPEAELAVVKLKPA
                     KKYLRDFYLIPSDAPAFQENDIMMGIKYLRMMADRLKKPLVILLAMGSNSGSHIGTSP
                     LSQVTQNYSGFFGIITVIAGGNETGAAHHFYASIPAGTEYEDVEIRVGKEEAERGFVL
                     ELWAADADTYTVGFISPSGERISRIPIIANNETSIPFLLDATTITVNYQLIEAESGSQ
                     LIFMRFQTPAAGIWTVRVYNTQRFKGTFHMWLPVQGMISEETVFLRPDPYSTITVPGN
                     SRLPITVGAYDTSTGGIYIHSSRGFTPNQIVKPELAAPGVNILGPSVGRKPGSDTPMT
                     TRTGTSAAAAITAGAAANLLGWGIVEGNYPTMSEASVKSYLIRGAQRNPALTYPNREF
                     GYGTLDLFQTFLRLRE"
     gene            complement(12291..13673)
                     /gene="clpX"
                     /locus_tag="ACZPCG_02850"
     CDS             complement(12291..13673)
                     /gene="clpX"
                     /locus_tag="ACZPCG_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003501409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HIR1284042.1"
                     /translation="MEKNDVSRSEGKDREDEGYEKVCYMCRRPESKTGPMISMPGGMN
                     LCHDCMQKAFDSVMKNGMDLSQIPNMPYMNLNFNDLSQMPPKAEIPKKQKLKKSKEPQ
                     PQLAMKDIPAPHVIKEKLDAYVIGQEKAKKVMAVAVYNHYKRAFLGKEGSDSDDIVIE
                     KSNILMIGPTGSGKTYLVKTLAKLLDVPLAIADATSLTEAGYIGDDIESVVSKLLAAA
                     GNDVERAQKGIIFIDEIDKIAKKKQTNTRDVSGESVQQELLKLLEGSTVEVPVGSNQK
                     NAMTPMATVNTDNILFICGGAFPDLEGIIKERLTKKSAMGFGAVLKDSFDHDPDILSH
                     VTNEDLRTFGMIPEFLGRLPITVTLKGLDKDMMIRILQEPKNAILRQYEKLLALDEVK
                     LVFEEDALEWIAEEAIRRGTGARALRAILEEFMLDIMYEIPKDPNIGSVVVTRPYLEK
                     NGGPLVQMRG"
     gene            complement(13721..15067)
                     /locus_tag="ACZPCG_02855"
     CDS             complement(13721..15067)
                     /locus_tag="ACZPCG_02855"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR1284043.1"
                     /translation="MYEEDKDRNWNREPEEERPKKGGILKKAAWITAGALLFGTIAGG
                     VMVGVQIASSGVFKNLYAIVSGEPEAGDWKAPEGQKPPADPFAGPETATPSYIAAPVT
                     DVSVIVEEAMPSVVAITSTAVYQSRDFGYDWFFGGGLGGQTYEVPSSGSGIIIGENET
                     ELLIVTNNHVVEEATSLKVTFIDEETVDAVTKGTDPETDLAVIAIPLDQIKEETKAQI
                     HPAVVGNSDELKPGQGVIAIGNALGYGQSVTVGYISAINREVQTEEGNTRVLLQTDAA
                     INPGNSGGALLNMKGELVGINAAKYASTEVEGIGYAIPISKSQDIIDQLTKRRSKVDI
                     PEAERGYLGIQGTNVDENAAKTFGMPRGAYVYKILESGAAASSELWEKDIITKLDGQT
                     VRNMEGLQEILAGYRAGEQVELTVQRQIDGVYQELTVTVTLAAMPAEEQAAASGQQ"
     gene            complement(15203..17212)
                     /locus_tag="ACZPCG_02860"
     CDS             complement(15203..17212)
                     /locus_tag="ACZPCG_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIR1284044.1"
                     /translation="MNRDEFEFGFDPEEEQEARRPKAGNTGHTFVPAFRQAETEGKRP
                     RRSTAFRREQEEDSYMDLDSDFDEEEEIEFGAGSHRESTGRRTVPEREKSTGYRAGRS
                     TDKVRSRRAGGRGSGGPKRFFGGLTLRGLILLIAAQITAFAVIGCVWWGKRMWSNMQT
                     DTEFNQADMTNPYISVEKEEQMKGYWTVALFGVDSRNNSVGKGNMSDVIILCTVNQET
                     GAIKLVSVFRDTYLNIDEKNSYNKINLAYSRGGPKQAVEALNRNLDLQIDDYATFNWK
                     AVADSINILGGVDVELSKAEFYYINSYITETVESTGVGSHHLKKAGPNHLDGVQAVAY
                     ARLRKMDTDYARTERQRKVIELSFDKLKKADFAVVNNVMEVVFPQILTSITLNDMIPM
                     AKNLTKYHIAATAGFPEARSDGILGKKGACVIPQTLESNVIKLHQFLFDDENYVPSEM
                     VKKISAKISADTGMYKEGKPIESVGTNGGYVPKATTAAQTKEETKENGTETSEGSSES
                     TKHGNEDVIDGEYPWGLELETDENGNVIDPPENYGPGMLPSESSGSTHSGQSKPGAST
                     ESSGSLKPTVPSGTLPSATTEAVTKPAKGTDAGSEGPGAVESGDADSGPGAASQPSQT
                     SPVNSGAGQSKPGQTESSASGVIPGAGNVIIEGNPENDVIIGPGA"
     gene            complement(17231..18655)
                     /locus_tag="ACZPCG_02865"
     CDS             complement(17231..18655)
                     /locus_tag="ACZPCG_02865"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HIR1284045.1"
                     /translation="MIKDNQKKLNRIHVLLDVVMTVLAYFLAWLIIVSGKVIPLEYNV
                     KTLEPPVYFAALIFIIPVYLVLNACFHLYVPKRIQGRRSEFANICKANVIGLMFFTLI
                     LFGGRNIIIHLGYFSTRMLLCFFVLNILLLEAERMAIRLFLRSLRTNGYNQKHILLVG
                     YSRAAEGFIDRVALNPEWGYHIQGILDDGRPSGYAYKNVRILGTINQLESVLAANTLD
                     EIAITLSINEYSNLESIVAVCEKSGVHTKFIPDYNNIIPTIPYMEDLQGLPVIHIRHV
                     PLTNVFNATVKRIVDIGGALFGLLLFSPVMLLTAGLIKLTSPGPIFYSQERIGLHNRP
                     FKMYKFRSMEVQDPGKEKTRWTTPHDPRVTPVGRFIRKTSIDEMPQFYNILIGDMSLV
                     GPRPERPLFVEKFKEEIPRYMIKHQVRPGLTGWAQVNGYRGDTSITKRIEHDLYYIEN
                     WSLGFDFKIMFLTIFKGFINKNAY"
     gene            complement(18789..19778)
                     /locus_tag="ACZPCG_02870"
     CDS             complement(18789..19778)
                     /locus_tag="ACZPCG_02870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004605728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1284046.1"
                     /translation="MSNRIKVTVIIPNYNGLKFMEPCFKALELQIYHKFQILVVDNGS
                     TDGSVEWLKEHQIPTIFLDTNTGFSGAVNVGIRAAKTPYVLLLNNDTEPDCHFIGEMV
                     KAIERSPKIFSVSSKMIQMYHKDKMDDAGDMYSLLGWAYQRGVGRSSGGYSHPCRVFS
                     ACAGAAIYRREIFEKIGYFDEMHFAYLEDIDVGYRARIAGYDNVYCPHAVVYHVGSGT
                     SGSKYNSFKVRLAARNNIYLNYKNMPFLQLLVNVLPIGAGMTVKYLFFKKRGFGADYL
                     AGVKEGLATAGKCKKVGYRPEHLKNYLAIEWELFTGTLLYFYEFSLRQLEKRK"
     gene            complement(19775..21997)
                     /locus_tag="ACZPCG_02875"
     CDS             complement(19775..21997)
                     /locus_tag="ACZPCG_02875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009532608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1284047.1"
                     /translation="MKYKIDIVRIRENSITLNGWVLGKSPESRATFRVEDERHNPVKY
                     KLVTTRRDDVSQIYFKKTYDRDFGFDIQFPYERGKTYWLLIRCEGRQAKIKYNEELIA
                     KRSSVAHKRMEKIKDLMNMETVRVAADFWKENGLKALIKKSCHKLQGLDNDYDYSEWY
                     ELTKPTEEELKVQREARFEYEPLLSVVIPVYRTPEHYLREMIDSIIDQTYTNWELCIA
                     DGSPRGQSVERVLKRYAQKDPRVRYAVLGGNRGISGNTNAALEMASGDFVILADHDDT
                     LPEHAFYEVVKAVNEHPDCEVLYSDEDKLDMDGKALFDPHFKPDFNPDLLTSVNYICH
                     LFVVKKSLLDQVGGFRKEFDGAQDYDFIFRCTEKAAKIVHIPKVLYHWRCHQGSTASN
                     PESKMYAFEAGSRAVMAHYERVGIPAESVEKGVDYGIYHTHFKIQGEPLVSVIIPNKD
                     HSQDLDTCIRSLMERSTYQNLEFIVVENNSTSPETFAYYKKLQEAHSNVKVVTWKEGF
                     NYSAINNFGASFASGEYLLLLNNDTELLEPDSIKEMLGFCQREDVGIVGARLLYADDT
                     IQHAGVVVGFGGIAGHTFIGLHKAENSYFHRAMCAQDYSAVTAACLMTKKSVFETVGG
                     LSTELAVAFNDIDYCMKVRALGKLVVYAPYACLYHYESKSRGLEDTPEKVARFNREVA
                     MFHKKWPEILEQGDPYYNPNLTLRKSNFALRDLLKEKIGEPYDLSVYDAYGPEEKEGK
                     "
     gene            complement(21987..22964)
                     /locus_tag="ACZPCG_02880"
     CDS             complement(21987..22964)
                     /locus_tag="ACZPCG_02880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003506680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1284048.1"
                     /translation="MKKISILIPCYNEVENVGPISQAVTDILKNELPQYDYELVFIDN
                     DSTDGTREVLRTLCSSDPKIKAIFNARNFGQFNSPYYGMLQVTGDCVIEMVADFQDPV
                     EMIPKYVREWENGYKIVIGIKTSSKENRFMYWLRTCYYKTIKKMSDVEQIEHFTGSGL
                     YDREFIEILRRLDDPTPFLRGIVAELGYKRKEIPYEQPKRRAGKTHNNFYRLYDAAML
                     SVTSYTKEGLRLATIFGGVCAAVSMLIALVYLVMKLIWWNRFPAGMAPMLIGMLFLGS
                     VQLFFIGFLGEYIMSINQRVMKRPLVIEAERLNFDEKPEEKEEGKKDEV"
     gene            complement(23004..23921)
                     /locus_tag="ACZPCG_02885"
     CDS             complement(23004..23921)
                     /locus_tag="ACZPCG_02885"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR1284049.1"
                     /translation="MRIVITGATGFIGSYLAALFLREGAEVFALSRPDSPNLKALPVH
                     ENLHIVPWSMDRAGDCVSQIGSADAFFHFAWGGVNRQEIDSPEVQAKNVAGSLECIRA
                     AKALSCSVFMDAGSRVEYGLTDGIMSEDRECAPINQYGKAKWEFYQKAAPLCKELGLH
                     YFHLRFFSVYGPGDHPWSIISTLVRELPQGKSVSLSACLHQWNFMYIEDAVQAVYELY
                     RHLPSEKDSPKISEIVNVASRDTRVLRSFVEEVWKEAGGRGSLEYGSFVQAKEGALSV
                     RPSVEKLAALTGGFKEAYSFSQGIRQMLP"
     gene            complement(23933..25066)
                     /locus_tag="ACZPCG_02890"
     CDS             complement(23933..25066)
                     /locus_tag="ACZPCG_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR1284050.1"
                     /translation="MKRCIACGAPLWETPLLTLDNMPASAQHMPDKDHVDEDQGLTLD
                     LCQCMGCGLVQFDCEPVDYYRDVIRAGGYSTTMAELRRKQYRHLIETYHLEGKRFIEV
                     GCGQGEFLKVLEEFPVEGHGIEHDPALVKLARAQGLAVEQGFTEHADTVIPGGFYDVF
                     LSFNFLEHQPDPAGMLRCIYNNLEDDGMGLLTVPSFEYIMDHSSYYELIRDHIAYYTF
                     DTLTSLLERTGFVVEEAEVINRDTLAVIVRKRPQMETGNLLDCYVNLRREMDTYMKYL
                     DAWKKKAAIWGASHQGFTLAATTSLGKKAEYIIDSAPFKQGKFAPASHLPIVAPSYFE
                     EHPVDAVIITAPGYTDEIAALIREKFGPEVEIRAMRSNHLEML"
     gene            complement(25067..26020)
                     /locus_tag="ACZPCG_02895"
     CDS             complement(25067..26020)
                     /locus_tag="ACZPCG_02895"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR1284051.1"
                     /translation="MAVIVTGATSFIGRAVVEELLKQGQKVYAVARPGSAGLSFLKAL
                     PLNGQEGRREGAVGSIRILECNLNEIERLKEMDITDASAWLHLGWEGAGSANRQDPAL
                     QAKNIGYALSAVRTAGALGCSRFLFSGSQAEYGICDSPMREDQPCHPVSEYGKDKVLV
                     CRQAGELAGQLRMDYIHTRIFSVYGPGDHPWSLISTCIDTFLKEGHMEMGPCTQQWNF
                     LYVTEAAELLVKLLLGKAPAGVYNVAGEDTRPLRDYIEELHRLCGSLGSYHFGDRPPN
                     AEGMVSLMPELTKLKAAVGFTQQIPFSEGIRRMVQEKKGEI"
     gene            complement(26072..>26452)
                     /locus_tag="ACZPCG_02900"
     CDS             complement(26072..>26452)
                     /locus_tag="ACZPCG_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015572912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR1284052.1"
                     /translation="FLINNGGYHSIRQTQKNFFGEPLIGIGVDSGDLSFPDMEKLAGA
                     YGYPYIKAEHNSELAKAVEQTLAVEGPVICEIFVSMDQNFEPKSSAKRLPDGTLVSPP
                     LEDLAPFLPEEEMDRNMIIPRIRE"
BASE COUNT         6465 a         7384 c         5580 g         6923 t
ORIGIN      
        1 cattatcccg gacgtccttc cagccgatct gcaaaatgcc gtcctcgtca aagcagtaat
       61 acgttccgtc aatcttgcgg gtaccgtaat catctccgga attgtcatca ctgggaacat
      121 atttctttcc attggtgcca aaatagaacc agtagcagcc gtcctccccg tactccacat
      181 ttcccgtatc gtatccggga actaccttgt tgatctcttc atcatattca tccggaggaa
      241 tcagcttctg ccagcctgta cgcatcacgc cgtcatcacc ggtataatag gtgtcatcca
      301 ggatccaccc ccgctccatg gcgccgttgc tgtcaaaatg ataccacttg tccgaaattt
      361 ttttccaggt atcctctacc ttctttccgg aagccgagaa ataataccat tcgtcttctc
      421 catcatcctc aggcacatga agccattttt ctgtaaccat gataccgttg ctgtctacat
      481 agtattctcc gtccacccag gagttgaccg ccatggtccc gtttccgtcc agataacgcc
      541 actgcccatc cgctcccttt ttccaatcgt tgtagacttt atttccattg ctgtcataat
      601 agacccagtt tccctctgct tccgcccagc cttctgccca cgctgtcaga ttgactgtac
      661 cagccgacag aagaaccgcc gcaaaacaca ttgccgtcat cttcctcttc atacacgcct
      721 ctttctcctt aaacgccttt ctttcttgaa agaaatgctt ctttaagacc ctcatctgcc
      781 ttctattgta tcaggggttc tgcgttcttt caaccggaat tacctttcaa ttctattacg
      841 aaaaacgtct gcggcatttg aatctccccc gcggtccgtt tttaacatat tcccctcaaa
      901 aaagaaacgg gccggactcc cagacggaag ccagcccatt ttctattaaa tactttgatg
      961 aacgattcga ttatttcagc atcttcttca tctcatctgc aacgaactgt acctttgtac
     1021 ccacaattac ctgaatggaa ttcttgcctg ggcggatcac accagctacg cctgcggatt
     1081 tgatcttctt ctcatctacc acactctgat ccttgatctc cagtctcagt cttgtgatgc
     1141 agttttccag agattttaca ttttccttgc cgccaagtcc ttccagaatc acagaagcaa
     1201 cctctgtata attgttgttt gccagaacag cagacttctc agcctccaga tcatcatcat
     1261 cctcgcggcc cggagtcttt aaatcaaact ttacaatagc aaagcggaat accacataga
     1321 ataccaggaa tgctgcgata cccagcggaa ggatcatcca tgtcttctga gctgccggaa
     1381 gggatgcgga gaagaacagg tcagttgcgc ccgcggagaa gctgaagcct gcgcggaatc
     1441 caagaatagt tgtaactgtg gtgaaaattc cgtacagcag agcgtaaact acatacaggc
     1501 cgggagccag gaacataaat ccaaactcaa agggttctgt aacaccgcag acaaatgcgc
     1561 tgagggctgc agaagcaacc agaccgatcg caactttttt ctttgtgctc tttgctgtct
     1621 gaaccatagc caaagcagcg cctgggatac cgaacatcat acatgggaag aaaccggaca
     1681 tatacatacc aaggctccat gtaacatctg cagatgtctc gcctgcccag aaatgggaca
     1741 gatcgcccag accaatggta tcaaaccaga atacattgtt taatgcgtga tgcagaccgg
     1801 ttgggatcag cagacggttt aagaatgcat aaatacctgc gccgatcgcg cccatgcctg
     1861 caataccgtt tccgattgca accagaccgc cgaacagaac cggccatacg aaaagcagga
     1921 taacagaggt gataattgcc actacgcctg caatgatggt tacgcagcgc ttgccgctga
     1981 agaaggacag ccagtctgga agctgtgttc ccttaaactt gttgtagcag gtagaaccga
     2041 tgataccgga aagaataccg ataaatgggt ttgcgatctt gctgaatgcc agagtcgtct
     2101 gctgatcctc agcgatcgac ggcatcaggg tagtcactac gcctgtggat aaaagagagg
     2161 tgatcatcag ccaggaagcc agagctgcaa gtccgcctgt accgtcatga tcctcagata
     2221 acccaacacc cacaccgatt acaaacagga tcgccatatt gtcaatcaga gcgccgcctg
     2281 cctttaccag aaggaaacca atctgctgaa ggattccttc aacctcgccg ccctgcatgg
     2341 tagacgggca gagataatat ccgattccca tcaggatacc acagatcggc agacatgcta
     2401 caggaagcat cagggcctta cccagttttt gtaaatactt catactatgt actcctttct
     2461 tttatgggaa atttcctttt cgaatactct cccatacttg acctaatgga tgatccattt
     2521 gtgaaaataa aaaaccgttt tgtctgatgg acggacacga attaacaatt catttataat
     2581 tcctcaacaa atacggcctt tttacattga aaaaattttc tttttaatca ataactttca
     2641 ctggattttt ccatttctac tgacataata tcaacatttt ataaaaaagt caatctgatt
     2701 ttatactttt ttaataattc atacagaaac tttcaattgt cttttcttta tcttttgaaa
     2761 cccacagcac agcgcttgac gatgcagtat tatttggact atactcatta ggaatcaatc
     2821 acaaaaacag cgccgtgcgc cgacacgggc actatataat tctgaaagaa atgaggttta
     2881 tcaatttatg tggcttgcag atggatggaa cgattatgaa gtgatcgact gctctaaggg
     2941 cgaaaagctg gagcgctggg gggactatct cttagtgcgt cccgaccccc aggtaatctg
     3001 ggatacgcca aaaactcacc gcggatggaa aaaaatgaac gggcattacc accgcagcag
     3061 caaaggcgga ggcgaatggg aattttttga cctgccccag cagtggacca ttaattacaa
     3121 ggaactgacc ttcaatttaa aacccttcag ttttaagcac accggcctgt ttcccgagca
     3181 ggctgcaaac tgggactggt ttggtgaaaa gatccgcagg gccggacgtc ctgtcaaggt
     3241 attaaacctg tttgcctaca ccggaggcgc taccctggca gccgccaagg ccggagccag
     3301 cgtcactcat gtagacgctt ccaagggcat ggtaacatgg gccaaggaaa acgcccattc
     3361 ctccgacctt gcagacgccc ccatccgctg gatcgtagat gactgcgtta aattcgtaga
     3421 acgcgaaatc cgcagaggga accactacga cgccatcatc atggatccgc cctcctatgg
     3481 gcgcggtcca aaaggagaaa tctggaaaat cgaggatgcg atccatcctc tggtaaagct
     3541 gtgcgtacag ctcttatccg aagatcccct tttcttcctc atcaactcct acaccaccgg
     3601 ccttgcccct tccgttcttt cctatatgat gggcgtagaa atcgtaccga aattcggcgg
     3661 aaccgtcaaa gccgacgaag taggactgcc cgtatcccaa aacggactca ctctcccctg
     3721 cggagcttcc ggacgctggg agaagtaaga gaaatgctgg agaaatgctg gagaaacgcc
     3781 ggaagaaaca tatctccgtt caggcgcgct ctcgctcggg gaaatacgca gcggtactaa
     3841 gctcgaaaaa acctcgctaa gtaccggcgt ctttccacgg ccttcaccag agatatgttt
     3901 cttccggctg gtggctcctt gaatagagta ataaggtgct ctctggcata ggtttttcag
     3961 aaagtcaagg gaattaccat ccctcccgaa gggtattccc aagcagaagc catcctctcc
     4021 aggcgcattg cggaggggcg tgtctgtctt ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nngcttctgc ggcccctact gcagcagctt ctgcacatcc aacgcccccc
     4201 atccctgctg atttctggga agacccagat ctatggcccg ttctctgagg cggagtttta
     4261 catcccggtt ggtcatatct gggtattttt ccagcaaaag ggcaatggct cctgatacca
     4321 aaggcgtaga catactggtg cctgatttgg caaaatatgc tcccgcctcg ttcccgcagc
     4381 ttacgatccc gcatcccggc gctacgatat ccggcttgca aatgcagctg gcagtagggc
     4441 cacggccgga gtaatctacc atcctgtttc ccatcacctc tacttcctta tggtcgtctg
     4501 aacagcctac cgttatgact tttcggctga ttcccggcgt ggtgactgtc atagggccgg
     4561 ggccgtgatt tccggctgcc acaacgacca ccaggccatc atcccaggcc gcgtttaccc
     4621 ctttaacaag ggcagaattt tccgtcatat ctctctttga cagagaacct accgaaatat
     4681 ttacaatccg gattcccaaa aactctttgt gggagcggat ccagcggagt ccggaaagca
     4741 catctgaagc atatccattt ccccgatggt ctaatacctt tacggagata atagagcagc
     4801 caggagccac tccccggtat tttccttcca tggaagcccc gctccctccg atcatggcgg
     4861 caacatgggt tccatggcca ttgtcatcat agggtctgtc tttcctgcgg acaaaatccg
     4921 caaatccctt tactctatcc tttaaatcct catggagaaa gattcccgta tccaggacag
     4981 ctacgcctat gccccgtcct gtgatcccca tggaataaac ctggtcgcag tgaatatcca
     5041 ctctggctcg attcacatct taaatactcc ttttttgtta gcctattcca cagagacatt
     5101 ccatgtgcag cctgcactgt tccctgaaac agaaaaaact ccgtacacct ggtacggagc
     5161 ttttttctta ggatttagga ttttcaaaaa gggttgaagg atagttatta aactttcgtg
     5221 catttcttat gtacttagta tatcatgcca tccctgaaaa tgtttgaatg ttctttgaat
     5281 agattattaa gaaaatctta attatccttt cttaaaggaa gccagacgaa ttttcagccg
     5341 ctctctgtca ccgtctgcaa aacagtatcc atccatgcca caggcttttg caccctgaat
     5401 attccgttcc agatcatcga taaagaaaca ttcctcaggc tttaaaccaa aacgttcaaa
     5461 aaaatgctca tacatttcct tctgaggctt catgcatttt acttctgcgg aaaagaaaat
     5521 accgtcaaag cagtcaatgg ccgggattac ctgatgatag caggtcaaaa ggcgcaccga
     5581 tgcattggaa caaagataga tgcggtaccc ctcttccttt aattccctaa caagttcttc
     5641 catcccctct ttatggtaca tattgtattc atgccagtgg gcaaagcttt ttctcgccag
     5701 ttccttctcc tcctccgtat ccagccgcgt cagtatctgg cttaaacctt cttcctcgga
     5761 aataatcccc atatccagca taagccattc cggagataca aatacagccg tgctgacctt
     5821 gcggatcaga gcctcgtctt ctgaaaaatg gcggcagacc cggtctccct catattccac
     5881 cagtaccttt cccatatcaa atacgatatt tttgatcatg attcgtctcc ttatttacgt
     5941 aaaaagggat gccgaaaacc cgtaagcttt ccggcatccc cctcagtgac atttattttg
     6001 tctccggctt ttcaacctga acgatagcaa ccttctgcat cggaatacgg cagtttttgt
     6061 tgctgccaaa ctctacgatc actgtgtcat cggtcatatc gatgatcact ccgtaaaatc
     6121 cgctgcttgt caggatggta tctccaacag caacgcttgc aagcagctcc tgctgtttct
     6181 gtctctcttt tttctgggga cgaattgcca taaaataaac aagtgctccc aggaaaataa
     6241 tatagagaat aattcctccc attccagcca tggtgtttcc tccttcatac ctgctgctac
     6301 attgccccag cagtttcttt tatcaatcct tctgacctgc catgccggcc agcttagcgg
     6361 ctttgtactc gctgtagcgg tgttcttcaa tggcttcccg gatctcttcc atcattttat
     6421 tataaaaata caaattatgc aaggtacata atctcattcc cagcatttct tttgccttta
     6481 acagatggcg tatatatgca cggctgtagg aacggcaggc aggacactga cagccttcct
     6541 caatcggccg gtgatccagc tcatatttct ggttgaacag attcagtttg ccatgattgg
     6601 tatacacatg accatgacgt ccgtttctgg acggataaac acagtcaaag aaatcaaccc
     6661 ctctgtctac agcctctaaa atattggcag gagtacctac tcccataaga taaaccggtt
     6721 tttcctccgg cagatgagga acagttacat ccagaatgtg gtacatttcc tcatgagtct
     6781 cgcctacggc aagtccgccg atggcatagc cgtccagatc catctcacgg atcgttttgg
     6841 catggtcaat acggatctca tcaatcacac cgccctggtt aataccaaac agaagctgtt
     6901 cccggtttac tgtatccgga agagaattta aacgggtcat ctctgattta caccgcttca
     6961 gccagcgtgt tgtacggtct acagactttc tgatatagtc ataatctgca tggcttggcg
     7021 gacattcgtc aaaggccatg gcaatggtag aacccagatt ggactggatc tgcatactct
     7081 cctcagggcc cataaagatc ttatgtccgt caatatgaga gctgaaatat acaccctctt
     7141 cctttatctt tctcagatct gtcagagaaa atacctgaaa accgccggaa tctgtaagaa
     7201 taggcttgtc ccagttcata aacctgtgca gtcctccaaa ttcccggatc agcttatctc
     7261 cagtacgtac atggagatgg tatgtgttgg acagctctac ctgagtgccg atctggcgca
     7321 gatcgtctgt agatacagca cccttgatcg cgcctactgt tcctacattc ataaacaccg
     7381 gagtctggat tgtaccgtga actgtttcca tcaccgcacg cttagcccgc ccatcctttg
     7441 taattacctg atatttcata aattattcct cgtcagccag agctgctttg atcatgatcg
     7501 cgcactcgat ccgttttttc tgcatctcgc agccgatctc atatgcgccg ttaatggaac
     7561 cgctgaaatt gtaggaattg gcactgcagc cgccgctgca gtagaatctg gcaaagcaat
     7621 ccttgcagct ttcctttgca tatacgttgc aaagcttaaa ctcattgcgt atttcctcat
     7681 ttacaatgcc cgtatctacg ttgcccagaa ggaactcctc atttcctaca aactgatggc
     7741 aggggtagag atcaccccat ggagtaactg ccagatactc tgtacctgaa ccacagccag
     7801 ccatacgctt tgcaacacag gggcctgcat ttaagtcaat cataaagtgg aagaagttga
     7861 agcctcttcc ttctttttta cgcttgatgt attctactgc cagcttatca tactcctcaa
     7921 ggatctgggg aatatcttcc tcacggatag cgtagtccat ctcaggttct gcaacaaccg
     7981 gctccatgga catctgctta aagcccagat ctgcatagtg aagcacatcc tttgaaaaat
     8041 ccaggttgtt tctggtaaag gtgccgcgga catagtaatc catctggcct ctgctctcag
     8101 caaacttctg gaactttggt acgatcaggt catagcttcc tgaaccgttt cggaaaggac
     8161 gcatcttatc attgacctcc ggacgtccgt ccaggctgag aaccacatta ctcatctcgc
     8221 ggttgcagaa ctccataatc tcatcattca ggagcacgcc attagtagta agcgtaaaac
     8281 ggaaacgctt attgtgagcc tcctcctggg agcggccgta ctctaccagc tgcttcacaa
     8341 ccttccagtt taaaagaggc tcgccgccga agaaatcaac ctccagattc cgtctgtttc
     8401 cagaattggc aatcaggaaa tccagagcct tttttcccac ctcaaagctc ataagagcac
     8461 ggcggccgtg atactcaccc tcatctgcaa agcagtagcg gcaggccaga ttgcagtcgt
     8521 gggcaatatg aagacaaagg gcttttacaa ctgtctttct cttcttaaaa tccattacaa
     8581 aattcctgta aatatcctgg gtcagaagct gctctgcatc aatgagctgc tggatatcct
     8641 ccagagcctc tgtgacctct ttttcactgt aggtaccatc cagggcttca tgaaccctgt
     8701 cggtcacttc tgccggaaga gcctgtggct tttccagttc cggaatctgc tctgccaccg
     8761 ccttgatggc gtcatagaca accggatcta ccacatggac ggaaccgctg tttacgtcca
     8821 gaacaatatg ataaccattg ttaatatact gatgaatcaa aatttatact ctctctttct
     8881 aagggctgtc tgccccatat cttttacagc aaaggaaagc ccctataatc ccggtgacca
     8941 taggggcttt gcagcatagg aaacggacgc cggatcagcg gtcagcgttc tcgcagctct
     9001 ggtttccaac tgtacaggaa gtcttgcatg cagactggca ggaagtctgg cactcgccgc
     9061 atccgccctt cttcatcgta tcctttaagg tattggtatt caaagtctta acgtgcttca
     9121 tatcagagta cctccttttt tccttgtttc tgtccctgag gatatgcccc agaaaccgtt
     9181 atcgtatgaa ttataacaca gctttcaggg gcttgcaatg ggattttgct cctgcctgtc
     9241 atatttgtct tctcaatggg atatatttac accatatcaa tcataccagg agggatccct
     9301 atggaaaacg gcattgtaaa acccatgctc cgctcccttt tgatctccta cgtcctctca
     9361 gggcttctgc tcggcgctct ggcctttgcc ctgtataagc tgcgtttaaa ggaaggacag
     9421 gtaaatctca tggtctatct cgtctatctg gccgcctgtc tggcaggcgg agcagcagca
     9481 ggaaaacgtc tgcgccagag aaggttcttc tggggactgc tgtcaggact tttttacttt
     9541 ctcatcctct tcgcggtctc ctgggccatg aacccctggt ccgcccttga cacagaacgc
     9601 tccgtcaccg taatggctgt ctgcgccctg ggagggatgc ttggcggaat gttttctgcc
     9661 taaatcaatc tactttcttt ttaaactcct ctcccatttc gcctaaaaga aacaaagctg
     9721 tacccaaaaa tacaaacaga tccccaagat taaataccac cttcttaatc cctggtagtg
     9781 aaaatgaaaa atagtccacc acatatcccc gtttaaaacg gtcatacagg ttgcttaacg
     9841 cgcctcccaa aaccatagaa agccccacct tctgaatccc gcagcccttt ctcaccaaaa
     9901 gcatggaaag cgcgccggcc acagcagagg aaacagccag aggaaccgct gttaccagct
     9961 gtggcttttc ctttaaaaaa ccaaaaggaa acccataatt atgattcctg tgaatacgga
    10021 tccgtccctt cgctcccggc agatcccttg gaaactcccc atccgtttcc tgctcgatct
    10081 cactcttcat cccctgatcc aaagccgcaa tccctcccac aagccaaatc agatacttca
    10141 tgccaatccc tcctctgtca aatttaatct ctatcctcat tctacaacat cccccaaaaa
    10201 acatctaccc atttccccac caatttctca accctcccac ctcccccagc cagaagtgag
    10261 gtttctgtcc tgccttctga gcgactttgc aggctgttag aatgacttag aggataaaat
    10321 ttttcgttaa gagccatatc aaacctgtcc gagcgcagcg agtttttgat atggctcttg
    10381 cttttagaaa aatttcagcc tcttagacat tcttacagcc ggaaaccgga gctcagaagg
    10441 caggacagaa accggactcc ccgcatatac taatcaaaac cctcttccgg agccgtcctt
    10501 cccatgcccg actgcccttt taatcccgcc gctgaaaaca tagccgatct gatctaccgc
    10561 ttcggatctc tgcaatctga acgctggaac aacacgtcct ccctgtgtgc agactatgtg
    10621 agcagcgatt atatcattct ccatatgcct ctcactcctg atgccgtatc catgcgtgta
    10681 cactcctact atatgattcc ctcgctgttt tcccctttgg attacaatgc catggaggca
    10741 tcaggaatcc tctcagcctt taactcccct gcacttaaca accagggaaa gggcgttctc
    10801 attggcctgg tagacacagg aattgattac cgctctccct tatttcaaaa tcccgacggc
    10861 acaacccgta ttgccggaat ctgggatcag tcggttccca tcgaggaaga tgtactgcca
    10921 gctggtgtgc cggattacta tccaatggga ggagcctcct acggcacaga atacaccaga
    10981 gaacagatca acaaagctct cgcctctgac gatcccctct ccctggtttc cacacaggat
    11041 accataggac acggcacctt tctcgccgga ctggctgcag gcagctccat acctcaggag
    11101 gattttacgg gagcagcgcc agaggcagaa ctggcagttg taaaattaaa gcctgcaaaa
    11161 aaatatctcc gcgacttcta tctgatccct tcggatgctc ccgcctttca agaaaatgat
    11221 attatgatgg ggatcaaata cctgcgcatg atggcagacc gtcttaaaaa gcctctggtg
    11281 atcctgctgg ctatgggttc aaactccggg agccatatcg gcacctcccc attaagccag
    11341 gtcactcaga attacagcgg tttttttggc atcatcaccg ttattgcagg cggaaatgaa
    11401 accggagcgg cccatcattt ttacgcttca atccctgcag gcactgaata tgaagatgtg
    11461 gaaatcagag tgggaaaaga agaagcagaa cgcggcttcg tgctggagct gtgggctgcg
    11521 gacgctgata cctacaccgt aggctttatc tctcccagcg gagaacggat cagccgtatc
    11581 cctattatcg ccaataatga aacttctatc ccttttcttt tagacgctac aactatcact
    11641 gtaaactacc agctcattga ggccgaatcc ggcagccagc tgattttcat gcgcttccag
    11701 acgcccgcag ccggtatctg gacggtgcgt gtatacaata cgcagcgttt taagggaacc
    11761 ttccatatgt ggcttcctgt acagggaatg atttccgagg aaaccgtctt tctgcgtccc
    11821 gatccctaca gcaccatcac cgttcccggc aactcccggc ttcccattac cgtaggcgcc
    11881 tatgatacgt ccaccggagg catctacatc cactccagcc gcggctttac tcccaatcag
    11941 atcgtcaaac cggagctggc cgctccgggc gtcaacatcc ttggcccttc cgtcggcaga
    12001 aagccaggaa gtgatacgcc catgacaacg cgcaccggaa cctcggcagc ggcggcaatc
    12061 accgctggag cggccgccaa tcttttaggc tggggcattg tagaaggaaa ctatcctacc
    12121 atgagcgagg catctgtaaa aagctacctt atacggggag cacaaagaaa tccagcgctc
    12181 acatacccga accgtgagtt tggatatgga acgctggatc tgtttcaaac ctttcttcgt
    12241 ctgcgggaat agccatcctg cagcatgaga gcaatattgt atgccaaatg ctaacctctc
    12301 atctgcacca gaggtcctcc atttttttcc agatacggcc tggtaactac cacggaacca
    12361 atattgggat ctttggggat ctcatacatg atatccagca tgaactcctc caaaattgcc
    12421 cgcagcgctc tggctccggt gccgcgccgg attgcttcct ctgcaatcca ctccagagca
    12481 tcctcctcaa agaccagctt cacctcatcc agtgccagaa gcttctcata ctggcggaga
    12541 atagcatttt tcggctcctg cagaatacgg atcatcatat ccttatccag tccctttaac
    12601 gttaccgtta tgggaagacg gcccaaaaac tccggaatca tgccgaaggt tctcagatcc
    12661 tcgttggtta catggctcaa aatgtcagga tcatgatcaa aggaatcctt aagcactgct
    12721 ccaaagccca tggcggactt cttcgtaaga cgctccttaa tgatcccctc aaggtcaggg
    12781 aacgcgcctc cgcagataaa caggatgttg tctgtattta ccgttgccat gggggtcatg
    12841 gcatttttct gattggagcc tacaggtacc tccactgtgc ttccttccaa aagctttaaa
    12901 agctcctgct gaacagattc accgcttaca tccctggtat tggtctgctt ctttttggca
    12961 atcttatcaa tttcatcaat aaagataatc cccttctggg cccgctccac atcattgcct
    13021 gcagccgcca gaagcttgga taccacactt tcgatgtcat cgccaatata tccggcctct
    13081 gtcagagagg tagcgtctgc gatcgctaac ggtacatcta aaagctttgc aagcgtcttt
    13141 accagataag tctttccgct gcccgtcggt ccgatcatca aaatgttgga cttttcaatc
    13201 acaatatcgt cactgtcaga gccttctttt cccagaaatg ctctcttata atggttatat
    13261 acggccactg ccataacctt tttagccttt tcctgtccga tcacataggc atccagcttt
    13321 tccttaatca catgaggcgc aggtatatcc ttcatggcaa gctgaggctg aggctcctta
    13381 ctctttttaa gcttctgctt tttggggatc tcagcctttg gaggcatctg ggacagatca
    13441 ttaaagttca gattcatata gggcatattg ggaatctgag aaagatccat cccgtttttc
    13501 atcacagaat caaaagcctt ctgcatacag tcatggcaca ggttcatgcc tcccggcatg
    13561 gaaatcatgg ggcccgtttt actctccggc ctgcggcaca tatagcagac cttttcatat
    13621 ccctcatcct cccggtcctt tccttcggac cgggaaacgt catttttctc tatctctttc
    13681 tcttccaatg taaatctcct ctctcatggg caatataaac ttattgctgc ccggatgccg
    13741 ctgcctgttc ctccgcaggc attgctgcca gtgttacggt aaccgtcagc tcctggtaca
    13801 ctccgtcgat ctgtctctga acagtcagtt ctacctgctc tccggcccgg tagcctgcta
    13861 aaatctcctg aaggccctcc atgttccgca cggtctggcc atctaatttg gtaataatat
    13921 ccttctccca cagctcagag gaggctgctg cgccggactc caggatctta tatacataag
    13981 cgcctctcgg catgccaaag gtctttgccg cattttcatc tacattcgtt ccctggattc
    14041 ccagatatcc cctttccgcc tcaggaatat caacctttga tctgcgcttt gtaagctggt
    14101 caataatatc ctgggatttt gaaataggga tggcataacc aatgccctct acctcggtag
    14161 aagcatattt tgccgcatta atcccaacta gctcaccctt catatttaaa agagcgccgc
    14221 cgctgtttcc aggattgatc gccgcatctg tctgaagaag tacgcgggta tttccctctt
    14281 ccgtctgcac ttcccggttt atggcgctga tatagccgac tgtcacagac tggccgtagc
    14341 caagggcatt tccaatggcg atcacaccct gtcccggctt taattcatcc gagtttccca
    14401 caactgccgg atggatctgc gctttggttt cttctttaat ctgatctaaa ggaatggcaa
    14461 tcactgccag atccgtttcc ggatccgttc cctttgtcac agcatctacc gtttcctcat
    14521 caataaatgt aacctttaag gatgtggctt cctccaccac atgattattc gttacaatga
    14581 gaagctctgt ctcattttcc ccgataataa tgcccgatcc gctgcttggc acctcatagg
    14641 tctgtccgcc cagtccgccg ccgaaaaacc agtcatagcc gaaatcacgg ctctgatata
    14701 cggctgtgct tgtgatagcc actaccgaag gcattgcctc ctccacgatg acagacacat
    14761 ctgtaaccgg agcagcgata taagacggcg tcgcagtttc gggcccggcg aacggatctg
    14821 ccggaggctt ttgcccttcg ggcgccttcc agtcccctgc ttccggttcc cctgaaacaa
    14881 tggcatacag attcttaaaa acgccggagg acgcgatctg tactcccacc atcacacctc
    14941 ctgcaatggt accaaacagc agcgcccccg ctgtgatcca ggctgccttc tttaaaatgc
    15001 ctcccttttt aggtctctct tcttcaggct ctctattcca attacgatcc ttatcttctt
    15061 cgtacatttg ctgtatctcc ttccttcact tatgtgacta tcttataatg aaacagaaaa
    15121 aatatcaaat aaataattga taaacaaacc gccggggaaa tcgtcatttt tccccggcgg
    15181 tctgccgtat catcccttat tctcaggctc ccggaccgat aattacatcg ttttccggat
    15241 ttccttcaat aatcacattt cctgctccgg gtataactcc tgaggcggaa gactccgtct
    15301 gtcccggttt tgactgtcct gctccggaat tgaccggact tgtctgagaa ggctgagacg
    15361 ctgctccagg cccgctgtct gcatctcccg attcaactgc accgggtccc tcagagcctg
    15421 catctgtacc cttagcgggc tttgtcactg cttccgtcgt ggcagaaggc agtgtcccgc
    15481 tgggtactgt cggcttcagg ctgcccgaag actctgtcga tgctcccggc tttgactggc
    15541 ctgaatgcgt gctgccggag gactcagacg ggagcatccc aggaccgtaa ttctccggcg
    15601 gatcgatcac atttccgttc tcgtctgtct caagctccag tccccacgga tattcaccgt
    15661 caatcacatc ctcattccca tgcttcgtgc tctcgctgct gccctcggag gtttcagtcc
    15721 cgttttcttt cgtttcttcc tttgtctgag ctgccgtagt agccttcggc acatagcctc
    15781 cgtttgttcc cacgctctcg ataggctttc cttccttgta cataccagta tcagctgaaa
    15841 ttttggcgct gatctttttt accatttcac ttggtacata attctcatca tcgaataaga
    15901 actgatgaag cttgatcaca ttgctctcca atgtctgtgg aatgacgcag gcgccctttt
    15961 tgcccaaaat cccgtcactt ctggcctctg ggaatcctgc tgtagctgca atgtggtatt
    16021 tggtaaggtt tttagccata ggaatcatat catttaaggt aatactggta aggatctgcg
    16081 gaaataccac ttccattaca ttatttacaa ctgcaaagtc cgctttcttc agcttatcaa
    16141 aactgagctc gatcaccttt ctctggcgct ctgtacgggc ataatccgta tccatcttgc
    16201 gcagtctggc atacgctacc gcctgaacac cgtccagatg gttcgggccg gccttcttta
    16261 aatgatggga accaactccg gtggattcta ccgtttctgt aatataagaa ttgatatagt
    16321 aaaactctgc cttgctcagc tctacatcca caccgcccag aatgttgatg gaatctgcca
    16381 cagccttcca gttaaaagtc gcataatcat caatctgtaa atccagattc cggttcaggg
    16441 cctctaccgc ctgctttggg ccgcctctgg aataagctaa attgatctta ttataggaat
    16501 ttttctcgtc tatgttcaga taggtatctc tgaatacgct caccagcttg atggcgcccg
    16561 tctcctgatt gactgtacag agtatgataa catcagacat attccccttg cctacagagt
    16621 tgttccggct gtccacgccg aaaagagcca cagtccagta ccccttcatc tgctcctctt
    16681 tttccacaga aatataggga tttgtcatat ctgcctgatt aaactccgta tccgtctgca
    16741 tattggacca catccgcttt ccccaccaga cacagccaat aactgcaaaa gccgtaatct
    16801 gagctgcgat cagaaggata agccccctga gggtaagtcc gccaaaaaac cgctttggcc
    16861 cgccgctgcc tctgccgcct gcccgtctgc tgcggacttt atccgtactt cgtcccgctc
    16921 tgtaccctgt gcttttttcg cgctctggaa cggttctccg gccggtactc tccctgtggc
    16981 ttcccgcccc aaactctatt tcctcttctt cgtcaaaatc actgtccaga tccatatagg
    17041 aatcctcttc ctgctctctg cggaaagcgg tgcttcgtct gggacgtttt ccctctgtct
    17101 cagcctgacg gaaggcaggg acaaaggtat gacctgtgtt ccctgccttt ggcctgcgtg
    17161 cttcctgttc ttcctccgga tcaaatccga actcaaattc atcgcgattc atatgttcat
    17221 cctcactgca tcagtatgca tttttattga taaagccttt aaagattgtc aaaaacataa
    17281 tcttaaaatc aaaaccaagg ctccagtttt cgatgtaata cagatcgtgt tcgatccgtt
    17341 tggtaatgga agtgtccccc cgatagccgt ttacctgagc ccagcctgtc agcccgggcc
    17401 gcacctgatg cttgatcata tagcggggaa tctcctcctt gaacttttcc acaaacagcg
    17461 gccgctccgg ccttggccct accagactca tatccccgat caggatattg taaaactggg
    17521 gcatctcgtc aatactggtt tttcgtataa agcggcccac cggcgttacc cttgggtcat
    17581 ggggcgtagt ccagcgtgtt ttttcctttc ccggatcctg gacctccata gaacggaact
    17641 tgtacatctt aaagggcctg ttgtggaggc cgatccgctc ctgactgtag aaaatggggc
    17701 ccggagaggt cagctttatg agccctgccg taaggagcat caccggagag aacaaaagaa
    17761 ggccgaaaag agctcctcca atgtccacaa tccgctttac cgtagcgtta aatacgttcg
    17821 tcagaggcac atggcggata tggatcaccg gcagtccctg aagatcctcc atatagggaa
    17881 tcgtaggtat aatattattg taatccggaa tgaatttggt atgaacgccg gatttctcac
    17941 agactgccac gattgattcc agatttgaat actcattgat actcagggta atggcaattt
    18001 catccagcgt atttgctgcc agaacgcttt ccagctggtt tatggttccc aggatcctga
    18061 catttttata ggcatatcct gacggtctgc cgtcatccag gatcccctgg atatgatatc
    18121 cccactcagg atttaaagcc acccggtcaa taaagccttc cgccgcccgg ctgtagccca
    18181 ctaaaaggat gtgcttctga ttatagccgt tggtgcgcag agagcgcaga aaaaggcgga
    18241 tcgccatacg ttccgcttcc agcaaaagga tatttaaaac gaaaaagcaa agaagcatcc
    18301 tggtggagaa atatcccagg tggataatga tatttcttcc gccaaacagg atcagcgtaa
    18361 agaacataag gccgatcaca ttggccttgc agatgttggc aaactctgat ctgcgtccct
    18421 ggatccgctt cggcacataa aggtgaaaac aggcatttaa caccagatac accggtatga
    18481 taaatataag cgccgcaaaa taaacgggcg gttccagagt tttcacattg tattccagag
    18541 ggatcacttt tccgctgact atgatcagcc aggcaagaaa atatgccaga accgtcataa
    18601 ccacatccaa aaggacatga atccggttca gcttcttttg attatctttt atcatggaaa
    18661 atcacctgat attcagtctc atccggcaga accctttctt caggcactct gccgggtgct
    18721 cctattatag cttatttaaa ctcaagattc tataacattt atcttaaaat atgataaaat
    18781 tcgccttttt acttcctttt ttccagctgc cttaaggaaa attcgtagaa atacagaaga
    18841 gtacctgtaa ataattccca ttcgattgcc agatagtttt tcaaatgctc cggtctgtat
    18901 cctacctttt tacatttccc tgctgttgcc aacccttcct tcacgcctgc aagataatcc
    18961 gctccaaagc cgcgcttttt aaaaaacaga tatttcacag tcattcctgc tccaatgggc
    19021 aggacgttga caagcagctg caggaaaggc atatttttat aatttaggta aatattattc
    19081 cgggccgcca gccgcacctt aaaggaatta tacttggaac cgctggtgcc gctgcccaca
    19141 tggtacacaa ctgcgtgagg acagtaaaca ttatcatagc ctgcaatcct ggcccggtag
    19201 cctacgtcaa tgtcctctaa ataagcaaaa tgcatctcgt caaaataccc gattttttca
    19261 aagatctccc tgcggtaaat ggcagctccg gcgcaggcgg aaaagactct gcaggggtgg
    19321 ctgtagcccc cggaggaacg tcctacgccc ctctgatacg cccagccaag gaggctgtac
    19381 atatctcctg catcatccat cttatccttg tgatacatct ggatcatctt gctgctgaca
    19441 gaaaaaatct taggcgacct ctcaatggcc ttaaccattt caccgataaa atggcagtcc
    19501 ggctctgtat cattgtttaa cagaagcaca tagggcgtct ttgccgccct gatccccaca
    19561 ttaaccgccc cggaaaaacc ggtattggta tccagaaaaa tcgtggggat ctgatgctcc
    19621 ttaagccact ccacactgcc gtccgtggag ccgttgtcca ccaccaggat ctgaaatttg
    19681 tggtaaatct gcagctccag cgccttaaag cagggttcca tgaattttaa tccgttataa
    19741 ttgggtatga tcacagtgac cttgattcga ttgctcattt tccttccttt tcctccggcc
    19801 cataggcatc gtacacagac aggtcatagg gttctccgat cttctccttc aagagatcac
    19861 gaagggcaaa gttagacttg cggagcgtga gattggggtt gtagtaagga tctccctgct
    19921 ccaggatctc cggccatttt ttatggaaca tggcaacctc ccggttaaag cgggccacct
    19981 tttccggcgt gtcctccaat cccctggact tggattcata gtggtaaagg caggcataag
    20041 gcgcatatac taccagtttc cccagcgccc gcactttcat acagtaatca atatcattaa
    20101 aggcgactgc cagctctgtg cttaagcctc ccacagtctc aaagacggat ttctttgtca
    20161 tcagacaggc tgcagtgact gcgctgtagt cctgtgcaca catggcacgg tggaagtagc
    20221 tgttctcagc cttgtgaagg ccgataaagg tatggcccgc aatcccgcca aagcccacta
    20281 ccacgcctgc atgctggatg gtgtcatctg cataaagcag tcttgctccc acgatcccca
    20341 catcctcacg ctggcagaag cccagcatct cctttatgct gtccggctct aacagctctg
    20401 tatcattatt taacaaaaga agatactcgc cggatgcaaa ggatgctccg aaattattga
    20461 tggctgaata gttaaagccc tccttccatg tcactacctt aacattggaa tgagcctcct
    20521 gaagcttttt ataatatgca aaggtctccg gactggtact gttgttctcc acaacgataa
    20581 attccagatt ctgataggtg gaacgctcca tcagagaacg gatacaggta tccagatcct
    20641 ggctgtggtc tttattggga atgatcacag acacaagggg ttctccctga atcttaaaat
    20701 gcgtgtggta aatgccgtaa tccacaccct tttccacgga ttctgccggg attcccactc
    20761 gctcatagtg agccataacc gctctggagc ctgcctcgaa agcgtacatc ttactttccg
    20821 gattgctggc tgtggacccc tgatggcagc gccagtggta gagcaccttt ggaatatgaa
    20881 cgattttggc ggctttttcc gtacaccgga aaataaaatc ataatcctga gcaccgtcaa
    20941 actccttcct aaagcctccc acctgatcca aaaggctctt ttttactaca aacagatggc
    21001 agatataatt gacactggta agaagatccg ggttaaaatc aggcttgaaa tgcggatcaa
    21061 acagcgcctt tccatccata tccagcttat cctcatcgga ataaaggact tcacagtccg
    21121 gatgctcgtt gactgccttt accacctcat aaaaggcgtg ctccggaagg gtatcgtcgt
    21181 gatcagccag gatcacaaaa tcgccggaag ccatttcaag agccgcattg gtattgccgg
    21241 aaatgcccct gtttccgccc agcaccgcat agcgtactct gggatccttc tgggcgtagc
    21301 gctttaatac ccgttccacg ctctgtcctc tgggactgcc gtctgcaatg cacagctccc
    21361 agttggtata ggtctgatca atgatggaat cgatcatttc ccgaagataa tgctccgggg
    21421 tgcggtagac gggtataacc acagaaagaa ggggctcata ttcaaaacgc gcttccctct
    21481 ggacctttaa ctcctcctct gtgggctttg taagctcata ccactcgctg tagtcatagt
    21541 cattatcaag gccctgaagc ttgtgacagg acttctttat cagagcctta agtccatttt
    21601 ccttccagaa atctgcagcc acacggacgg tctccatgtt catcaggtcc ttaatctttt
    21661 ccatccgctt atgagccacg ctggaacgct ttgcgatcag ctcttcatta tacttgatct
    21721 ttgcctggcg tccctcacag cggatcaaaa gccagtaggt ctttcctctc tcatagggga
    21781 actggatgtc aaagccaaag tccctgtcat acgtcttttt gaaataaatc tggctcacat
    21841 catctcttct ggtcgtaacc agcttatatt tcaccgggtt atggcgttca tcctctaccc
    21901 ggaaggtcgc cctgctttcc ggagattttc ccagaaccca tccgtttaat gtaatggaat
    21961 tttcacggat ccgcacaatg tctatcttat acttcatcct tttttccttc ctctttctct
    22021 tccggcttct catcaaaatt caggcgctcc gcttcaatca ccagaggccg cttcatgact
    22081 ctctgattga tgctcataat gtattcgccc agaaaaccga tgaaaaagag ctggacagag
    22141 cccagaaaca gcataccgat cagcatggga gccataccgg ccggaaaacg gttccaccag
    22201 atcagcttca ttaccagata aacaagagcg atcagcatac tgaccgcggc gcagacccct
    22261 ccgaaaatcg tagcaaggcg cagcccctct tttgtatagg aagtaacgct taacatggct
    22321 gcgtcgtaaa ggcggtaaaa gttattatgc gtctttcctg cccttctctt tggctgctcg
    22381 tagggaatct ccttgcgctt atagccaagc tctgccacaa tcccccgcaa aaagggcgta
    22441 ggatcatcca gacggcgcag gatttcaata aactccctgt cataaaggcc ggagccggta
    22501 aaatgctcga tctgctccac atcggacatt tttttaatag tcttgtaata acaggtccga
    22561 agccagtaca taaaccggtt ttctttgctg ctggtcttga tcccgattac gatcttgtag
    22621 ccgttttccc actcacggac atatttggga atcatttcca ccggatcctg gaaatctgcc
    22681 accatttcaa tgacgcagtc tcctgtcacc tggagcatac catagtaggg ggagttaaac
    22741 tgtccgaaat tgcgggcatt aaaaatcgcc ttgatcttcg gatcactgct gcacagagtc
    22801 cgcagcacct ccctcgttcc gtccgtcgaa tcattgtcaa taaagaccag ttcataatcg
    22861 tactggggaa gttcattttt caggatatct gttaccgcct ggctgatggg ccctacattt
    22921 tcgacctcat tgtagcaggg gatcaatatg ctgatctttt tcatttggat gtacatctct
    22981 ttctaaaata atcttgtaat ggtttatggg agcatctggc ggatgccctg ggaaaaggaa
    23041 tacgcttcct taaagcctcc ggtcaaggcc gccagcttct ctacggaagg ccgcacagac
    23101 agggcccctt cctttgcctg aacaaaggag ccgtattcca gacttcctct tcctcccgcc
    23161 tctttccaga cctcctccac aaaggagcgc aatacccgcg tatccctgct ggcaacattg
    23221 acaatctcgc tgattttcgg gctgtccttt tcagacggaa gatggcggta caattcatat
    23281 actgcctgaa ctgcgtcttc tatatacata aagttccact gatgaaggca ggcgcttagg
    23341 gaaacgctct ttccctgggg cagctcccgc accagagtgg agatgatcga ccaggggtga
    23401 tccccggggc cgtagacgct gaaaaaccgc agatggaaat agtgaagccc cagctctttg
    23461 cacagaggag ccgccttctg gtaaaattcc cacttggcct ttccatactg gttaatgggg
    23521 gcgcattccc tgtcctcgga catgattccg tccgtaaggc catactccac cctggagcct
    23581 gcatccataa aaacagagca ggaaagagcc tttgcagccc ggatacattc cagagaacct
    23641 gccacatttt tggcctgaac ttccggagaa tcaatctcct gacggtttac tccgccccag
    23701 gcaaaatgga aaaatgcgtc tgcagaacca atctgtgaaa cacagtcccc ggcccggtcc
    23761 atggaccagg gaacgatgtg caggttttcg tgaaccggaa gcgccttaag attaggggaa
    23821 tccggacggg acagggcaaa aacctctgcc ccttctctca aaaacagggc cgcaaggtaa
    23881 ctgccgataa agccggttgc ccctgtgata acgattctca taagctgtcc tcctagagca
    23941 tttccagatg attggaacgc atggcccgga tctccacctc aggaccaaat ttctcccgga
    24001 tcagggccgc aatctcatcc gtatagccgg gagcagtgat aatgacggca tccaccggat
    24061 gctcctcaaa ataggacggg gctacgatgg gaagatggga ggccggagca aattttccct
    24121 gtttaaaggg agcggaatca atgatatact ctgccttctt tccaaggctg gttgtagctg
    24181 ccagcgtaaa gccctgatga ctggctcccc agatggctgc ttttttcttc caggcatcca
    24241 gatacttcat ataggtatcc atctcccggc gcagattcac atagcagtcc agcagattgc
    24301 ccgtttccat ctggggacgc ttgcgcacaa taacggcaag cgtatccctg tttatcacct
    24361 ctgcttcctc taccacaaag cccgtacgct ccagcaggga agtcagcgta tcaaaggtat
    24421 agtaggcaat gtgatcccgg atcagctcat aatagctgct gtgatccata atatactcaa
    24481 agcttggaac ggtaagaagt cccatcccgt catcttccag attattgtaa atacagcgca
    24541 gcatccctgc cggatccggc tgatgctcca gaaaattaaa agataaaaat acatcataaa
    24601 agccgcctgg gatcaccgta tccgcatgtt ctgtaaatcc ctgctccact gcaaggccct
    24661 gagccctggc aagcttaacc agcgccggat catgctcaat cccgtgaccc tctaccggaa
    24721 attcctctaa tacttttaaa aattctccct gtccgcagcc tacctcgata aaccgctttc
    24781 cttctaaatg gtaagtttcg atcaggtgcc tgtactgttt tctgcgcagc tccgccatag
    24841 tggtggaata tcctccggcg cgaatcacat cccggtaata atccaccggc tcgcagtcaa
    24901 actgcaccag gccgcagccc atgcactggc agagatccag agtaagcccc tgatcctcat
    24961 ctacatgatc tttatccggc atatgctggg cagaagccgg catattgtcc agcgtaagaa
    25021 gcggcgtctc ccataaagga gcaccgcagg caatacaacg tttcatctaa atttctcctt
    25081 ttttctcctg taccatccgg cggatcccct ccgaaaaagg aatctgctgg gtaaagccca
    25141 ctgctgcctt taatttcgtc aattccggca tcagcgatac catgccctcc gcattgggcg
    25201 gcctgtctcc gaaatgatag cttcccagac tgccgcagag ccggtgcagc tcttctatat
    25261 aatcccgcag cgggcgggta tcttcccccg ccacattata cacgccggcc ggtgctttgc
    25321 caagaagaag ttttaccagc agctctgcgg cctcggtcac atagaggaaa ttccactgct
    25381 gggtacaggg ccccatttcc atatggcctt cctttaaaaa ggtgtcaatg caggtggaaa
    25441 tcaaagacca tgggtgatct cccgggccat agacgctgaa aatacgggta tggatataat
    25501 ccatgcgaag ctggccggcc agttccccgg cctgtcggca gaccagcacc ttatccttgc
    25561 cgtactcaga taccggatgg cagggctgat cctccctcat gggagaatcg cagatcccgt
    25621 actccgcctg agagccggaa aaaaggaacc ggctgcagcc aagggctcct gctgttctga
    25681 cagccgaaag cgcatagccg atgtttttcg cctgaagagc cggatcctgc cggttggcgc
    25741 ttcccgctcc ctcccagcca agatgcagcc aggcagaagc gtctgtgata tccatctcct
    25801 tcaggcgttc tatctcgttt aaattacatt ccagaatcct tatgcttccc acagccccct
    25861 ctctccggcc ttcctgtcca ttaagaggca gcgcctttaa aaaggacagg cccgctgaac
    25921 cgggccgtgc cacagcatac accttctgtc cctgttttaa aagttcctcc actacagccc
    25981 ttccgataaa gctggtagct cctgtgacga tcactgccat atggctgtct ccttttctgt
    26041 ctgttgttct gccggttctc ccaaccggga attattccct gattctggga atgatcatat
    26101 tccgatccat ctcctcctca ggaaggaagg gagccagatc ctccaaaggc ggggatacca
    26161 gagttccgtc cggcagcctc ttggctgagg actttggctc aaagttctga tccatggata
    26221 caaagatctc gcagatcacc gggccttcga cagccagcgt ctgctccact gcttttgcaa
    26281 gctcactgtt gtgctctgcc tttatataag gatatccgta ggcgcctgcc agcttttcca
    26341 tatccgggaa acttaaatcc ccgctgtcca ctccgatgcc gatcaaaggc tctccgaaga
    26401 aattcttctg ggtctggcgg atggaatgat agccgccgtt attgattaag aa
//
LOCUS       DBMDUH010000017        25603 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_1320_length_25603_cov_6.014352, whole genome shotgun sequence.
ACCESSION   DBMDUH010000017 DBMDUH010000000
VERSION     DBMDUH010000017.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 25603)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 25603)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25603
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1320_length_25603_cov_6.014352"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(70..1386)
                     /gene="cbiE"
                     /locus_tag="ACZPCG_02905"
     CDS             complement(70..1386)
                     /gene="cbiE"
                     /locus_tag="ACZPCG_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6y C5,15-methyltransferase
                     (decarboxylating) subunit CbiE"
                     /protein_id="HIR1284053.1"
                     /translation="MREDETYRQERPVVYLIGIGMGGEDQLTGQAIDRLEGAEAVMGA
                     GRMLGSVEPYIQGKPVLTAYKPSEMVQWLGSFSWTEAALVLSGDTGFYSGAEAASKAF
                     IREGWDVEYIPGISSLSYFCARIGRSWQDVYPVSSHGRECDIPAWVRTHKTCFVLLGG
                     EGGLSELCRSLVSSGLGHVDLWAGEKLSYREERILWKRKPAEILMEEEVHPFGSLACA
                     IVENPHAVEARIYPELHPRDSDFIRGKTPMTKESVRRLSLETLRIGADAVCFDIGAGT
                     GSVSVEMGLAIRRQCGEGRVYAIEREPGALELIDANRRKFHGDWFGFHIVAGEAPEAL
                     RGLETPTHAFIGGSGGRMKEMIEVLLQMNPKVRIVANAISLETVTEILGCMKKYGFKE
                     GEILQIQASAVEQVGSYHMPKAQNPVYIAVMQHPAENGEELQWQDL"
     gene            complement(1383..2243)
                     /gene="cobK"
                     /locus_tag="ACZPCG_02910"
     CDS             complement(1383..2243)
                     /gene="cobK"
                     /locus_tag="ACZPCG_02910"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016994 - precorrin-6A reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HIR1284054.1"
                     /translation="MNENISLERTHEEMGKNRKLILFAGTTEGRILAGKLAEEGISGV
                     VCAATEYGKELLKEEMERVCSLRNRDGTGVKDSLKIRAGRLDEEEMEALIRRENASLV
                     IDATHPYADQATANIRKACSHVPNVKLLRCLREEGESEAAAPVREMASVKETVSWLSG
                     QEGNILLTTGSKDLEAFSQIPDFRKRVYVRVLPLEDSIRCCRMQGYEGRHIIAMQGPF
                     SAEMNRAHLREYNCRFLVTKDGGKTGGFEEKLKGAQEAGAEVLVIRRPFDQGISLEAV
                     IREVKEWKNS"
     gene            complement(2209..3789)
                     /gene="cobM"
                     /locus_tag="ACZPCG_02915"
     CDS             complement(2209..3789)
                     /gene="cobM"
                     /locus_tag="ACZPCG_02915"
                     /EC_number="2.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046026 - precorrin-4
                     C11-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-4 C(11)-methyltransferase"
                     /protein_id="HIR1284055.1"
                     /translation="MVHIVGAGPGATDLITVRGARLLKEADVIIYAGSLVNKELLELA
                     KEGAEVYDSARMTLEEVMAVIKRAWKEKKEILRLHTGDPCIYGAIREQMDAMDKEKIP
                     YEICPGVSSFCGAASSLGMEYTLPGISQSVIITRMAGRTPVPDRESIASFAVHQTSMV
                     LFLSAGMLRELSAELIKGGYPADTPAAIVYKATWPEEKIVRCEVKNLAEAAEAEGITK
                     TALIIVGEAVGQKDYERSKLYDPCFETEYRKAQNPEMGEACGKAEDEKTGDLRQKEGR
                     TGRLYVVGIGPGSAKGMTGEARQALERCQVIAGYTVYADLVRDEFPQKEYLTTPMTRE
                     EERCRMAIACCLEGKDTAMICSGDGGVYGMAGLLLELAEETPQVEICVVPGVTAACSG
                     AAALGAPLMHDFAVISLSDRLTPIETIWERVEKAAAADFVICLYNPRSKGRPDYLRQA
                     CERILKYRSPQTICGLAKRIGREGEETEILTLKELMEAEADMFTTVYIGNSSTKQIGG
                     HMVTPRGYERKYFSGKDA"
     gene            complement(3812..4996)
                     /gene="cbiD"
                     /locus_tag="ACZPCG_02920"
     CDS             complement(3812..4996)
                     /gene="cbiD"
                     /locus_tag="ACZPCG_02920"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016360235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase
                     CbiD"
                     /protein_id="HIR1284056.1"
                     /translation="MEQIFTYRNQKKLRWGYTTGTCAAAASLAAVLMLLGRKETELVS
                     LTTPKGVRLELEIEDIQMGENWVSCAVRKDGGDDPDVTNGMLVYSRAEYGTGSAEGEP
                     ENGYVFEREGVRIELMAGEGVGVVTKPGLNCPVGKPAINPVPRSMIFHQAERACRESG
                     FQGCIKLSVSVPGGKEQAEKTFNPRLGILGGISILGTSGMVEPMSERALLETIGLELR
                     QKRLAGCERLILTPGNYGTAFLRDHLGIELDQAVKCSNFIGDTLDLAVQEKLRQVLLV
                     GHGGKLIKLAAGVMNTHSSAADGRMEVLGAWGGALGASSERICRILEAVTVEQALFVL
                     DETPGLLEAVMSRVMERVEFHLKKRAGERLQAECIIFTNERGILGMTSGAEALLKEFE
                     TE"
     gene            complement(5076..6152)
                     /locus_tag="ACZPCG_02925"
     CDS             complement(5076..6152)
                     /locus_tag="ACZPCG_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HIR1284057.1"
                     /translation="MKLAVICFTKKGALIGRRLYTYFKAQGVNCEACVPERFLEQDWK
                     ADGMKSRGKENLTQWSGRQFSEKNAMIFIGAAGIAVRAAAPWIRDKFADPPVIVIDET
                     ARFVIPLLSGHVGGANELACELAAFLGAVPVITTATDRNGLFAVDLFAAGNGLVLTDR
                     KMAREISARILEGKTVGFISDFPTDGVPEGCAPYWQEYTIWITIKDRLPKEMPQGIKV
                     LRLVPRAVTLGVGCRKGIPFPVLKERVDACLKEAKIAPEAVKRLASVDRKKEEPAILQ
                     LAKERGLELCFYTAEELALVQGDFKESEFVRETVGIGNVCERAACAGGGRLILKKRTG
                     EGTAVAAACKALDVILCEKGFGER"
     gene            complement(6231..6449)
                     /locus_tag="ACZPCG_02930"
     CDS             complement(6231..6449)
                     /locus_tag="ACZPCG_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007712093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HIR1284058.1"
                     /translation="MEKSTHKKNFDPEHMSPQEQLKFEIAQELGLDAKVLEQGWRSLT
                     SKESGRIGGMMTKRRRQMKEAAGKEKDS"
     gene            6673..7413
                     /locus_tag="ACZPCG_02935"
     CDS             6673..7413
                     /locus_tag="ACZPCG_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HIR1284059.1"
                     /translation="MNIAIVDDCAQDSCHLSQLLGAYCSACSLALQVSTFTRAADFLR
                     SWKSGVFDLIFIDIFLDKDSDTASGIRLAEHIRREDTGCTIIFTTISPDFALKSYSLK
                     ALDYLVKPISTADFSQTMDYYLSMKAKRSSHYIEVKESRVMVKIPIDTILYTDYFNHY
                     IQIHLADRIVKTYMRFEDFSKLLLCYPQFISCYRNCIINLDKVVRLEKTSFVLTSGEH
                     LPITRSLRLQIHQQYADYQFRKINGGIE"
     gene            7410..8771
                     /locus_tag="ACZPCG_02940"
     CDS             7410..8771
                     /locus_tag="ACZPCG_02940"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF025854.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1284060.1"
                     /translation="MNLLNLQLSALLVLDPLSVILPFLWIFRLEWRYDAHRIAGILFG
                     YLFIICGLFLFFLRLLPGFSRHGLIWSLLLMAFSLSLCLLLTKVKIQAAAYTLFLFKN
                     VSDSASLLSRFLTVLITSHNPSIPHTACVRSLELLLIPAAVLIITAVIGTRLRQAVYY
                     TRFLPVWRRMLLIPAFFFLMYYMDMGLIYMPELRPDDYNYNVLLITLFWLICVYTVHI
                     TTLQILFLITQSFALKEEYRTMKVMSDALTSQTANLQINLEQLLKARHDFRHHLAILK
                     GLLGQKEYTAASDYLESYLGSQESCDTYTYCSNAPVNALLNYYLQTAREHSVNVTANV
                     CLPAALPMPDMDFCTILGNLLSNALEACLRQEGGIPAISVTINSVGQSMITLSIRNSY
                     SHEIKEQDGQFISSKRDGIGIGTSSVRYLTERYHGFLNFSHGNGIFEASVFLNPSMDR
                     AAP"
     gene            complement(8879..10897)
                     /locus_tag="ACZPCG_02945"
     CDS             complement(8879..10897)
                     /locus_tag="ACZPCG_02945"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002573467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase/30S ribosomal protein S1"
                     /protein_id="HIR1284061.1"
                     /translation="MNAQVKGAASFEIPEGRTVTVAKTAGFCFGVERAVNQVYEQIKA
                     VKEGKAEGPIYTYGPIIHNEEVVRDLEEKGVTVLDTEEDLKALPEGKGTVIIRSHGVS
                     RHIYDILKERGIRIADATCPFVKKIHRIVDEHAAAGDAVVIIGSPFHPEVEGIRGWGN
                     TYTTVIENEAEAQNYVVPEGKKLCVVSQTTFNYNKFQDLVEKILKKGYDSSVLNTICN
                     ATQERQVEAAKIASRVDAMIVIGGRHSSNTQKLYEICLKECKNTFYIQTLGDLDPDSV
                     SSVRSVGITAGASTPKKIIEEVHTRMAEQSFAELLKEEEESTKPIRTGEIVTGQVIEV
                     SKDEIILSIAGNKSEGVITRDEYSNDNSVDLTTKVQIGEEMEAKVIKLNDGVGMVSLS
                     FKRMAADRGNKRLEEAFENQEVLTSKVTQVLDGGLSVEVEGARVFIPASLVSDTYEKD
                     LSKYADQDIQFVITEFNPKRRRIIGDRKQLLVAEKAKLKEELFARIQPGDKVEGTVKN
                     VTDFGAFIDLGGADGLLHISEMSWGRVENPKKVFKSGDRVTVLIKDIQGEKIALSLKF
                     PEANPWAQAAEKYAAGNVVTGRVARMTDFGAFVELEPGVDALLHVSQISREHIEKPSD
                     VLKIGQEIEAKVVDFNEDDHKISLSVKALLAAPQNEDVADVDIDSYSN"
     gene            complement(10894..11607)
                     /gene="cmk"
                     /locus_tag="ACZPCG_02950"
     CDS             complement(10894..11607)
                     /gene="cmk"
                     /locus_tag="ACZPCG_02950"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006781096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0036431 - dCMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HIR1284062.1"
                     /translation="MEHFNIAIDGPAGAGKSTIAKLAAKRLGFVYVDTGAMYRTIALH
                     ILREGIDPQDEAAVSAACRNVNVTIAYKDGVQQVLLNGENVSGLIRAEEVGKTASATS
                     GYLPVREKLVELQKELAKRENVVMDGRDIGTCVLPDAPAKIYLTASVKVRAERRYKEL
                     TEKGIDADLREIERDIESRDYQDMHREHSPLMQAEDAVLLDSSDMTLDEVVRAIFVIA
                     AEKGCSAAVSALGNKGEDR"
     gene            complement(11623..12921)
                     /locus_tag="ACZPCG_02955"
     CDS             complement(11623..12921)
                     /locus_tag="ACZPCG_02955"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BaiN/RdsA family NAD(P)/FAD-dependent
                     oxidoreductase"
                     /protein_id="HIR1284063.1"
                     /translation="MNKEKHIIIAGGGAAGMIAAVAAAREGCKVSLYEKNEKLGKKLF
                     ITGKGRCNVTNACDMEELFGAVVTNRKFLFSSFYGFTNEDVMELFEEAGLPLKIERGN
                     RVFPCSDHSSDVIAALERMMRQAGVKVYLRQEIKGLAVRSEEDTDTGAVRQVCCGICL
                     EGPDGKEKEVPADRVILATGGVSYPSTGSTGDGHAWAKMLGHKVTDLLPALVPFEAAE
                     KEEVKALQGLSLKNIEIAVYNGKKELYREFGEMLFTHFGVSGPVILSASSFCAKAVSK
                     GALKLVIDLKPALSGEQLDERILRDFAEAKNRQFKNSLSHLYPAKLIPEIVRRSGIDP
                     DKQVNEITRQERRRLVEATKGLTFTLTGLRPFKEAIITQGGISVKETDPSTMESKRVK
                     GLYFAGELLDLDAVTGGFNLQIAWSTGWQAGKAAAKDTTL"
     gene            complement(13058..14377)
                     /locus_tag="ACZPCG_02960"
     CDS             complement(13058..14377)
                     /locus_tag="ACZPCG_02960"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HIR1284064.1"
                     /translation="MKKVSKFGGSSLASAKQFKKVGDIIRADKSRRYVVPSAPGKRND
                     KDEKVTDMLYACYDAASEGRSYKKILEKIRGRYEEIIEGLDLNLNLDHEFAKLEENFL
                     AGAGRDYAASRGEYLNGIVIANYLGYEFIDAAEVIFFDEQGNFEADLTDKELSERLSH
                     VERAVVPGFYGSRPDGSIKTFSRGGSDVTGSIVAKAVHADLYENWTDVSGFLVTDPRI
                     VEDPEVIETITYRELRELSYMGASVLHEDAIFPVRKEGIPINIRNTNRPEDKGTLIVE
                     TTCRKPRHTITGVAGKKGFCAINIEKAMMNTEVGFGRKVLSVFEKSGISFEHMPSGID
                     TMTIFVHQSEFEDHEQSVIAGIHRAVEPDVVELESDLALIAVVGRGMRSNRGTAGRVF
                     SALAHARVNVKMIDQGSSEWNIIIGVKNDDFEKAIRAIYDIFVLADL"
     gene            complement(14514..15293)
                     /locus_tag="ACZPCG_02965"
     CDS             complement(14514..15293)
                     /locus_tag="ACZPCG_02965"
                     /inference="COORDINATES: protein motif:HMM:NF012669.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HIR1284065.1"
                     /translation="MSGRKTDRLDRMRKMLMTENGITIREFAGKLDVSEITVRRDLKL
                     LEKQGCLRLVNGVAIYHAAEKTALYPEYNLNDECLVFPQKKERIGRRAASLIEANDVV
                     AIDSGSTAAYLARALPYDIHMTVLASSFNVLLDLNDHYNCDIICSGGYLYSNTKTFYC
                     PEGISMINRTCINKTFITAAGISEKMNVTCIAPHEMDLKRALMESGQTKILLADSSKF
                     GRIFPTAFSHLKDFDSIITDDELSDEWRSLIESQGIHLYCE"
     gene            15555..16616
                     /locus_tag="ACZPCG_02970"
     CDS             15555..16616
                     /locus_tag="ACZPCG_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1284066.1"
                     /translation="MELRLVDLVKDFSGTKAVDTGSFSISDGKLTGLLGPSGCGKSTL
                     LYLISGLLKPTSGRIFFGDRDVTELPPEKRGIGLVFQNYALYPHMTVAQNIAFPLVNR
                     KENRELIQQKTQQMAKLVQVEELLSRKPGQLSGGQQQRVAIARALIKEPDILLLDEPL
                     SNLDARLRLEMREEIRRIQLETGVTTIFVTHDQEEAMSITDEIVLMKKGVIQQQCAPI
                     DMYLQPVNRFVASFMGNPPIDFFHFPIKDGRLILSESYALPIRTTASGTVLTGIRPEA
                     WEPGPGIRVTIETLEQRGRDVLAGFTLAGAKGKALLSITDRVRTGDTVELSLKRKDLL
                     YLFNEANETRIDAEVAYES"
     gene            16606..17538
                     /locus_tag="ACZPCG_02975"
     CDS             16606..17538
                     /locus_tag="ACZPCG_02975"
                     /inference="COORDINATES: protein motif:HMM:NF012738.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1284067.1"
                     /translation="MKVKGFCRKPRPENPLGNTEYSLKAYFYLLPAALILAVFVFFPI
                     IMTLRMGFYESYVYLTDVGKGFGLASFRYVLKDPAFRSAVRNTLIITFIGVPITIVLS
                     LGSALFINSLGKGKGLFQTIYFLPYVTSTIAIGLVFRWLFHSDYGYINHVLSFFGLPP
                     QKWLTDPNLTTISVTIFTIWNGMAFKIVLFLAGLQKIDPQYYQAARMDETPPSRVFFR
                     ITMPLLSSTFWMVTIVSVIHAFKTYNEVYSMFGGNGAGPGNSAITIVYYIYDMFFNRS
                     QVHYASAAAIIFFIIILALTALQKWVSKKFVHYV"
     gene            17554..18375
                     /locus_tag="ACZPCG_02980"
     CDS             17554..18375
                     /locus_tag="ACZPCG_02980"
                     /inference="COORDINATES: protein motif:HMM:NF012738.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1284068.1"
                     /translation="MTEKLKKAAVWILLTAGALVMMFPFIWMLATSLKTLPEATAIPP
                     TLFPAKAVFANYAEAFTMAPFGRYLFNSILVAGLGTLLTLVITVLAAYAFTVFEFPGK
                     SSLFFCCLATMMIPAELLIIQNFITVSKLHWVNTFQGIIIPTIASGFYIYMMREYFLQ
                     TPSVLYKAAKIDGCTDWRYLWRIMMPINKNAIATIGILTFISQWNSFIWPLMVSKDDA
                     HRVMAIGLLHFKDAVSSQINLQMAGSTIVLLPMLIFYLIFRKQIIEGVSRGGIKG"
     gene            18413..19738
                     /locus_tag="ACZPCG_02985"
     CDS             18413..19738
                     /locus_tag="ACZPCG_02985"
                     /inference="COORDINATES: protein motif:HMM:NF024808.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR1284069.1"
                     /translation="MKNMTCLLLAGTAALSLAACGSESAPSQTSDGIVTKITKPVELQ
                     FWHSISNPVHAEVLDKLIAQFNDTVGKEKQITVTATFNGSSSDLYSNVIAAIKAETAP
                     DVTLALRPYVADYLQTDLVVDLTPYIQDETVGMPDYEDIFEGLRNGGTTYAKEGTYSL
                     PIHSYSEVLYYNTAFFEENGLTVPATWDELIETSKKIHEITGAPAFGWDNLAGSFMTL
                     VKQYGGRYTDPEGNIYFAEEDSETALKVLNLWKENVGSGIWRTAGEDMFFSGPFANEQ
                     IPMYVGDSVEASYIPAKNPELKWSTAPIPQVSDDTAANLSAGHVITALNQSGNPEKAY
                     ASYELIKFLTSKEANLAVVTAGTGYLPIRQSVADSEEYKAYVADGHDFLTAAVEQNDR
                     YFYEPAFTNDTTTSSAVTSAVRTMMQEVAENGTDPQTALDNLKQTVGLN"
     gene            19777..20355
                     /locus_tag="ACZPCG_02990"
     CDS             19777..20355
                     /locus_tag="ACZPCG_02990"
                     /inference="COORDINATES: protein motif:HMM:NF023428.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR1284070.1"
                     /translation="MEILYHHILTPCGEPADIIISALQKFIRRGKTEEAARAAYELYL
                     SGEELRDYLWQRLLVISAEDVGLGQPAAPSVVLSLYHASQKLGADSMDYPLFFVHAVR
                     YLCSCPKERGSSTLASLTKRRIRDRVPFPLPDYVFDMHTIEGQNRGRSYEHFISQAAV
                     VFPEAVLNDSTAGQNDRWEAELTERRKGEEDI"
     gene            20352..21629
                     /locus_tag="ACZPCG_02995"
     CDS             20352..21629
                     /locus_tag="ACZPCG_02995"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013799.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HIR1284071.1"
                     /translation="MNRTLVISIDALISADIERLKKLPNLGRIMKQASWAEDILCCYP
                     TLTYPCHVTISTGCWPDRHGICNNEKFQPLSKNRPEWYWFRKDIKTPTLIDYAKAAGL
                     TTAAITWPVMGDCGADYNIAEIWAPHEEDDPTPYFDLADSPKAKEIFERNKHMLRWMK
                     TPQMDEFAAQCAVDIIKEYRPDLMFLHLSYVDHQRHKLGVRSEQLDHAFAFVDEQLGR
                     VFDALDETGGLENTNIAVLGDHGQLYCDQMFHLNTVLRDMGFLTEENGTVTSWKIYAA
                     QCAFSAQIYRQPDVDPKLVYETLCKIRDQYPGSISQIFTKEQAADLHLTGEFDFVVEA
                     GDRTAFDKTANATEYFTSVAEIREYKLSVSTHGHLPEKGDKPPFILSGPDVIPGKVQK
                     GGYLVDEAPTILRLLGITENHMDGTPFTWMTRL"
     gene            21647..22462
                     /locus_tag="ACZPCG_03000"
     CDS             21647..22462
                     /locus_tag="ACZPCG_03000"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1284072.1"
                     /translation="MTPRFICFDLDGTLYLDSCVYLRMINHFFHDTPYRDWIFPVQEQ
                     MKQVLSGQSSLRCGQFVPKQKAEHPKTPEDLFDIPGTAALLLPDPSPWLDRTLYSYIS
                     DGWTLGMYLARRIGWEGEDFWKRFRLVRNDLIDPYWGPVPNPALPVLLKELKKQGIHV
                     VLCSNAQEANCIELLDYLGFDDSCFEELCFDADKPHTFPQRIAQWGRQFNLSPKEMLF
                     IGDQGYFDLYSGKTCGASTLLVSPWNAADAELWDHMVQTPKEMEAYLRELCLK"
     gene            complement(22439..23863)
                     /locus_tag="ACZPCG_03005"
     CDS             complement(22439..23863)
                     /locus_tag="ACZPCG_03005"
                     /inference="COORDINATES: protein motif:HMM:NF024242.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1284073.1"
                     /translation="MNEEQGLKNVIDYIEAHIRESLSAEEIASACYYSRSNTKALFKK
                     VFQYGMMEYAARRKLTQAAGELLSTSQSVCEIALEYGFASQEVFTRAFKKMWQETPAR
                     FRKRRRFWQLFPRQDFFCDDCGVFRRRFCLEDMRKMLEHREHTWIVCFDMEAPGIRYL
                     KTVFGGEGGERFVLDCLQRIEGEMREGDHLFRIAGDKFVLLASSEAYETVLDQAERVF
                     RRNGEKVHYQGNAVELAMFGGLIRLDQGDARSERLFEKLDGVLGRRWELTKRRRVQED
                     TDKRGSRGSCRFVYPEQGFPTGTVYHGYEIWKNELWDTDGTDSDRFWGYGADIKRERE
                     HLSFPLMCMKEENDCFEQNAPQIFWKLDQPGGRMLFPKGENWCVFQVMGSEGLVVREH
                     FMVIRGLKAEKTADGFTLSFEKLFLEAVRTKNGSWLRLNEGELKETVHDLGLGAEEYR
                     KICRTEREIRTRFKNSPLFQTKLP"
     gene            complement(23856..24683)
                     /locus_tag="ACZPCG_03010"
     CDS             complement(23856..24683)
                     /locus_tag="ACZPCG_03010"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009254242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1284074.1"
                     /translation="MAYIEAENLQKTFKVYERPKGAGAALRSLIHRTYTEKRAVDGIS
                     FSIQKGEMVGYIGPNGAGKSTTIKMLGGILVPDGGSVLCGGRVPWKERKENALHMGVV
                     FGQRSQLNWELPMEDTFELYRKMYRIPESIYKRNVECFTELLGMEEFLRKPVRSLSLG
                     QKMRAELAASLLHDPEVLFLDEPTVGLDVVAKDRIRKFLKELNREKQTTILLTSHDMM
                     DIDEICSRVILIDHGKKMLDEPLEDFYQIGRQVCRKEKAGLDEMVRSLYESPEGSYE"
     gene            complement(24710..25501)
                     /locus_tag="ACZPCG_03015"
     CDS             complement(24710..25501)
                     /locus_tag="ACZPCG_03015"
                     /inference="COORDINATES: protein motif:HMM:NF017946.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1284075.1"
                     /translation="MGVYGAFLKNGFLTQMAHRGQYAMRMLAKIMGWSTGMIMVLILL
                     ERFGQIGGWNREEILLLYAFDILSYSVAATFFMGSFGKLHRLIRQGELDLVLTKPADP
                     MIYLAASHVSAGYTSNYLMGGAVLVICLIRLDIALTPVHLFWLAADLLGASLIQAAGF
                     VFTAVPAFWIGESEGLYRLLYKNLTEFIQYPLSVYHRGIRELLTFVLPYAFINYYPVQ
                     YFIDRQEGICPGILRYLTPLVGCGVFLLSRLFWRLGLRAYSGAGS"
BASE COUNT         6086 a         7376 c         5273 g         6868 t
ORIGIN      
        1 ttcttttaaa aagctccaaa agcccgcaag taatcatggt tttcccactg ccgctggagg
       61 ctgctgttat cataaatcct gccactgaag ctcctcccca ttctctgccg gatgctgcat
      121 caccgcaata tacacaggat tctgagcttt tggcatatgg tagcttccca cctgctccac
      181 tgcagatgcc tgaatctgaa gaatctctcc ttctttaaag ccgtactttt tcatacatcc
      241 caaaatttcc gtcaccgttt ccagagaaat tgcgtttgcc acgatccgca cctttggatt
      301 catctgcaaa agcacctcta tcatctcctt catgcgtccc ccgctcccgc cgataaacgc
      361 atgggtaggc gtctccaatc cccgaagggc ctcaggagct tccccggcta cgatatgaaa
      421 cccgaaccag tcaccgtgaa attttctcct gttagcgtca atcagctcca gtgcacccgg
      481 ctcccgctca atggcatata cccggccttc tccgcactgc ctccggatgg ccagtcccat
      541 ttctacagaa acagaacccg ttccagcacc gatatcaaaa cagaccgcat ctgcgccaat
      601 acggagcgtt tctaatgaca gacgccgtac gctttccttt gtcatgggcg tcttgccccg
      661 gataaaatca gaatccctgg gatgaagttc aggatagatc cgtgcttcca ctgcatgagg
      721 attttccacg atcgcacagg ccaggctgcc aaagggatgt acttcttcct ccatgaggat
      781 ttctgccggt ttccgtttcc agaggatccg ttcctctcta tatgagagtt tttctcctgc
      841 ccacagatcc acatgaccca gaccgctgct cacaaggctg cggcaaagct ctgaaagccc
      901 cccttctcct cccaggagga caaagcaggt tttatgggtt ctcacccagg ccggaatatc
      961 gcactcccgt ccatggctgc ttaccggata tacatcctgc cagcttcggc cgattctggc
     1021 gcagaaatag gaaagactgg aaattcccgg aatatattcc acatcccagc cttcccgtat
     1081 aaaggccttg gaagcagcct ctgccccgct gtaaaagccc gtgtcgccgg aaagcaccag
     1141 agcggcctcc gtccatgaaa aggaaccaag ccactgaacc atctcgctgg gcttataggc
     1201 tgtaagcacc ggcttcccct gtatatacgg ctctacggaa cctaacatcc tgcctgctcc
     1261 catgacagcc tctgctccct ccaggcggtc aatggcctgc ccggtaagct gatcctcacc
     1321 gcccattcca atgccgatca gatagaccac aggacgttcc tgtctgtatg tttcatcctc
     1381 tctcatgagt ttttccactc ctttacttct cttataaccg cttccaggct tatcccctga
     1441 tcgaaagggc gccggatcac caaaacctct gctcccgctt cctgggcgcc ttttaatttt
     1501 tcttcaaagc cgcccgtctt tcctccatcc tttgtaacca gaaaacggca gttatattct
     1561 ctcagatggg cccggttcat ttccgcagaa aacggaccct gcatggcgat aatatggcgg
     1621 ccctcatacc cctgcatcct gcagcagcgg atggaatcct ccaaaggcag gacgcggaca
     1681 tagacccttt tcctgaaatc cgggatctgc gaaaaagcct ccagatcctt gcttcctgtg
     1741 gtaagaagaa tatttccctc ctgcccggac agccaggaga ctgtctcttt aaccgaagcc
     1801 atctcacgaa caggggcggc agcttccgat tccccttctt ctctgagaca ccggagaagc
     1861 ttcacattcg gaacatgact gcaggctttg cggatattcg ctgtggcctg atctgcatac
     1921 ggatgagtgg catcaatgac aagggaagca ttctctctcc tgatcagcgc ttccatttct
     1981 tcctcatcca gccggccggc cctgattttc aggctgtctt ttacccctgt tccatccctg
     2041 ttccttaatg aacacacccg ttccatttct tccttaagaa gctcttttcc atattccgtc
     2101 gcagcgcaca cgactcccga aattccttct tctgccagtt ttcctgccag aatccttccc
     2161 tcagtagttc ctgcaaagag gatcagtttc ctgtttttac ccatctcctc atgcgtcctt
     2221 tccagagaaa tattttcgtt catagcctct gggcgttacc atgtgtccgc cgatctgttt
     2281 tgtggaagaa ttgccgatat aaacggtggt aaacatatcc gcctctgcct ccataagctc
     2341 ttttaaggtc aggatctccg tttcttctcc ttcccgtccg atgcgctttg ccaggccgca
     2401 gatcgtctga ggagaacggt attttaaaat ccgctcacag gcctggcgca gataatccgg
     2461 ccgccccttg ctgcggggat tataaaggca gatcacaaaa tctgcggcgg ctgccttctc
     2521 aacccgctcc cagatggttt caataggggt cagccggtcg cttaagctga tgacggcaaa
     2581 gtcatgcatc agaggcgctc ccagggcagc tgcaccggag caggcagccg tcactcccgg
     2641 cactacacag atttccacct gaggcgtttc ctctgccagc tcaagcaaaa gacctgccat
     2701 gccgtataca ccgccgtctc cgctgcagat catcgctgta tcctttccct caaggcagca
     2761 ggcgattgcc atgcggcagc gctcctcttc tcttgtcatg ggagtggtca gatactcctt
     2821 ttggggaaat tcatcccgca ccaggtctgc atatacagta tatccggcaa tcacctggca
     2881 tcgctccaga gcctgcctgg cctctcctgt catccctttt gcagagccgg ggccgatccc
     2941 caccacatat aagcggcctg ttctgccttc cttttgcctt aaatcccctg ttttttcatc
     3001 ttccgccttc ccgcaggcct ctcccatctc cgggttctgc gccttccggt actctgtttc
     3061 aaagcacgga tcatacagct tagaccgctc atagtccttc tgtccgactg cctctcctac
     3121 gatgatcagc gctgtctttg taataccttc agcttctgcc gcctctgcca gattcttcac
     3181 ctcacagcgc acgatctttt cctccggcca ggtagcctta tacacaatgg cagcaggcgt
     3241 atctgccgga tatcctccct ttatcagctc tgcagacagt tcccttagca tccctgcgct
     3301 taaaaacaga accatagatg tctggtggac cgcaaaggaa gcaatgcttt cccgatccgg
     3361 caccggcgtt cttcccgcca tcctggtgat gatcacgctc tgggaaatcc ccggaagcgt
     3421 atattccatc cccaaagagg atgctgctcc gcagaaggaa ctaacgccgg ggcagatttc
     3481 ataggggatt ttctccttat ccatggcgtc catctgctct ctgatcgccc catagatgca
     3541 gggatcccct gtatgaaggc gcaggatctc ctttttttcc ttccaggccc tttttatcac
     3601 cgccatcacc tcttctaagg tcatgcgggc gctgtcgtaa acttcagctc cttcttttgc
     3661 cagctccagc agttccttat ttacaaggga acctgcataa atgatcacat ccgcttcctt
     3721 taaaagtcta gctcctctca cggtgatcag gtctgtggcc cctgggcctg ctccaacaat
     3781 atgtaccata ctgcatcctt tcattcaaat attattctgt ctcaaattcc tttaaaagcg
     3841 cctctgcccc cgatgtcatt cccagtattc cccgttcgtt ggtaaatatg atgcactctg
     3901 cctgaaggcg ttctccggct ctctttttca gatgaaattc cactcgttcc atcaccctgg
     3961 acatcaccgc ttccaaaagt cccggcgttt cgtccaaaac aaaaagggcc tgttccaccg
     4021 tcactgcctc caggatccgg cagatccgct cggaagacgc gcccaatgct cccccccagg
     4081 ctccaagcac ttccatacgt ccgtctgccg cggaggaatg ggtattcatc accccggctg
     4141 caagcttaat gagttttcct ccatgtccca ccaaaagaac ctggcgcagc ttttcctgaa
     4201 ccgccagatc cagggtatct ccgataaaat tgctgcactt gaccgcctga tccagctcaa
     4261 tccccaggtg atctcttaaa aatgcagtcc cgtaattgcc cggcgtaaga atcaggcgtt
     4321 cacagcctgc cagccgtttc tgccgcagct ccagccctat ggtttcaagg agggccctct
     4381 cactcatggg ttctaccatc ccgctggtgc ccaaaatgga aatacctccc aaaatcccca
     4441 gtctgggatt aaaggttttc tctgcctgct cttttccgcc tggaaccgag acagaaagct
     4501 taatacaccc ctgaaaaccg gattcccggc aggctctttc tgcctgatga aaaatcatgg
     4561 atctgggaac cggattgatg gcaggctttc ccaccggaca gttaagtccc ggctttgtaa
     4621 caacgcctac gccttctcct gccataagct caatccgaac gccttcccgc tcaaacacat
     4681 atccattctc cggctctccc tctgcgcttc ctgttccata ttctgcacgg gagtacacaa
     4741 gcattccgtt cgtcacatcc ggatcatccc ctccatcctt gcgcaccgca caggaaaccc
     4801 agttttcccc catctgaata tcctcaatct ccagctccag acggacccct tttggcgttg
     4861 taaggcttac cagttccgtt tccttcctcc cgagcagcat gagaacagcg gctagagaag
     4921 ccgccgccgc acaggtccct gtggtatagc cccaccttaa ttttttctgg tttctgtatg
     4981 taaaaatctg ttccatcgtt cacccgtttt tttcattctg ccaaaaagta taccatatgg
     5041 catttcattg cacaacgaaa aaatacggcc tagcactacc gttctccaaa tcccttttca
     5101 cacaaaatca catctaaagc cttacacgcg gctgccacgg ccgttccctc tcccgtcctt
     5161 tttttcaaaa tcagccttcc gccaccagcg caggccgccc gttcgcatac atttccgatc
     5221 cctaccgtct ctctcacaaa ttctgattct ttaaaatctc cctgcaccag agccagttcc
     5281 tctgcggtat aaaagcaaag ctccagtccc cgctcctttg ccagctgtaa aatcgccggc
     5341 tcctcctttt tccggtcaac ggaagccagc ctctttaccg cctcaggtgc aattttcgcc
     5401 tccttcaggc atgcatctac ccgttctttc agaaccggaa aaggaatccc ctttcggcag
     5461 ccaacgccta acgtcactgc ccgcggtacc agacgcagca ccttgatccc ctgaggcatt
     5521 tcctttggca gccggtcttt tatggtaatc cagatggtat actcctgcca gtaaggcgca
     5581 cagccctccg gtactccatc tgtggggaaa tcgcttataa acccaacggt tttcccctcc
     5641 aggatccggg cagaaatttc tcttgccatc ttccggtccg tcaaaaccag cccgtttcct
     5701 gccgcaaaga gatccactgc aaagagtccg tttcgatccg tagccgtagt gatcaccggc
     5761 actgcgccaa gaaatgctgc cagctcacag gccagctcat tggctcctcc cacatgaccg
     5821 gataacagag ggatcacaaa tcgggccgtt tcatcgatca caattaccgg aggatccgca
     5881 aacttgtccc ggatccacgg agccgccgcc cgtacggcaa tccccgcagc gcctataaag
     5941 atcatagcat ttttctccga aaactgccgg ccgctccact gggtcaggtt ttcctttccc
     6001 cggctcttca tcccatctgc cttccagtcc tgctctaaaa accgctccgg cacacaggcc
     6061 tcacagttca ctccctgtgc tttaaaatat gtatacaggc gcctaccgat caaagcgccc
     6121 ttctttgtaa aacagataac tgcaagcttc atcctctatc tcctgtcctt tctccggccg
     6181 ccgccttcgc ctagatctca tcctaagtat agcggatgaa gccccttctt tcaactatcc
     6241 ttttccttgc ctgcagcttc cttcatctgg cgtctgcgct ttgtcatcat tcctccgatg
     6301 cgtccgcttt ccttggatgt caggctccgc cagccttgtt ccaacacctt cgcatccagt
     6361 cccagctcct gtgcaatttc aaatttcagc tgctcctgcg ggctcatatg ctccgggtca
     6421 aagttttttt tatgcgtgct tttctccatg ctgccaaact cctttccttc cctttatcta
     6481 tagacaaaag ggattcatta aggaaaagtc tatccatatt tttacaatat attccgattt
     6541 ttcttgcttt ttgaactttt gtatattata ctgaaagtga agaaaatata tcgtttcata
     6601 atgaaaagcg aaaatttttt tcaccgcaat tcagaaagcg gccggccata gcagccggaa
     6661 gaaggggtta ctttgaatat tgcaatcgtt gatgactgcg ctcaggacag ctgtcattta
     6721 tcgcagctcc ttggcgctta ctgctctgcc tgttctctgg ccttacaggt atcgactttc
     6781 actcgtgcgg cggattttct cagatcatgg aaatccggcg tttttgattt aatcttcatt
     6841 gatatctttc tggacaaaga ttccgacaca gcatccggca tccgtctggc agaacacatc
     6901 cgccgtgaag ataccggttg taccattatc tttaccacaa taagcccgga ctttgcatta
     6961 aaaagctatt ctttaaaggc actggactat ctggtaaagc ccatttccac agcggatttt
     7021 tctcagacca tggattatta cctttccatg aaggcgaagc gctcttccca ttatattgag
     7081 gtaaaggaaa gccgggtcat ggtaaaaatt cccattgata caattttata caccgattac
     7141 tttaatcact acatacagat ccatctggct gaccggatcg taaagaccta tatgcggttt
     7201 gaggactttt cgaaactttt gctgtgttac ccacagttta tcagctgcta tcgcaactgt
     7261 atcattaacc tggacaaggt ggttcgtctg gaaaaaacct cttttgtcct tacgtccggt
     7321 gaacatctgc ctattaccag aagcctcagg ctccagattc atcagcaata tgcggattat
     7381 caattccgga agataaatgg aggtattgaa tgaacctttt aaatcttcag ctttctgcac
     7441 tgctggtgtt ggatcctctc tcagtgatcc ttccattttt atggatcttt cgtttagaat
     7501 ggcgttatga tgcccacaga atcgcaggta tccttttcgg atatctgttt atcatctgtg
     7561 ggctgtttct ttttttcctg cgcctgcttc ccggcttttc cagacacggc ctgatctggt
     7621 ctctgcttct tatggcattt tcactgtctt tatgcctcct tcttacaaag gtaaaaatac
     7681 aggcagctgc ctatacgctc ttcctcttta aaaatgtttc cgactccgct tccctgctgt
     7741 cccgctttct caccgtttta atcacctctc acaatccctc tatcccgcat accgcctgtg
     7801 tccgttctct ggaactgctt ctgattcccg ccgcagtcct catcatcaca gccgtcattg
     7861 gaacacggct gagacaggct gtttattata cccgttttct tccggtatgg agaagaatgc
     7921 ttttgatccc cgcctttttc tttctcatgt actatatgga tatggggctt atctatatgc
     7981 cggagcttcg ccctgatgat tataactaca atgttctcct cattaccctt ttctggctga
     8041 tctgtgtata taccgtgcac atcactaccc tgcagatcct ctttctcatc actcagagct
     8101 ttgctctgaa ggaggaatac cgaaccatga aggtaatgtc cgatgctctg acctcccaaa
     8161 cagccaatct tcagatcaac ttagagcagc tgttaaaggc acggcatgat ttccgccacc
     8221 atctggcgat cctaaaaggg ctgctgggac agaaggagta cacggctgca tccgattatc
     8281 tggaaagcta cctgggcagc caggaaagct gcgatacata tacctactgc tccaacgctc
     8341 cggtcaatgc actgttaaat tattatctcc agacagccag agaacactcg gtcaatgtaa
     8401 ctgccaatgt gtgccttcct gctgctctcc ctatgccgga catggacttt tgcaccatct
     8461 tgggaaacct gctctccaat gccctggagg cctgtctgag acaggaggga ggaataccgg
     8521 ctatctcagt cactataaac tctgtcggcc agtccatgat caccctgtcc atccgcaact
     8581 cctattccca tgaaataaaa gaacaggacg gccagtttat ctcctccaag cgggacggga
     8641 tcggcattgg cacctcttcg gtgcgctacc tcacagagcg gtatcacggc tttctcaatt
     8701 tcagccatgg aaacggcatt tttgaggctt ctgttttttt aaatccatcc atggaccgcg
     8761 cagcacctta aaatacatcg aaaattttct gctgtgcata cgccgctagg cgcaccatac
     8821 tacaacaagg ccccgcggaa aatccgcagg gccttattat ttcatgcctt attggctgtt
     8881 agttggaata ggaatcgatg tctacatcag caacatcctc attctgagga gctgcaagga
     8941 gagcctttac gcttaagctg atcttgtgat cgtcctcgtt gaaatcaacg accttagcct
     9001 caatctcctg tccgatcttt aatacatcag atggcttctc gatatgctct ctggaaatct
     9061 gggaaacatg aagcagcgcg tctacgcctg gctctaactc aacgaatgcg ccaaaatcag
     9121 tcatacgtgc tacgcggcct gttaccacat tgcctgctgc atacttctca gcagcctgtg
     9181 cccatggatt tgcctccggg aactttaagc ttaatgcgat cttctcgccc tggatatcct
     9241 tgatcagaac agttacccga tcaccggact tgaatacctt cttaggattc tctacacggc
     9301 cccagctcat ctcagagatg tgaagcaggc cgtcagcgcc gcccagatcg ataaatgcac
     9361 caaagtctgt tacgttcttt acagtaccct caaccttatc acctggctgg atacgtgcaa
     9421 ataactcttc cttcagcttt gccttctcag caactaaaag ctgctttctg tcgccgatga
     9481 tacgtcttct ctttggatta aactctgtga tcacgaactg gatgtcctga tctgcgtact
     9541 tggacagatc cttctcataa gtatcagata caaggctggc tggaataaat actcttgcgc
     9601 cctcaacctc aacgcttaat ccgccgtcta atacctgagt aaccttggaa gttaaaacct
     9661 cctgattttc aaatgcctcc tccagacgct tgttgcctct gtcggctgcc atacgcttga
     9721 aagataagga aaccatgcct acgccgtcat ttaacttgat aaccttagct tccatctctt
     9781 caccaatctg tacttttgta gtcagatcca cactgttatc gttgctgtac tcgtcgcggg
     9841 tgataacgcc ctcggacttg ttgcccgcga tgctcagaat aatctcatct ttgctcactt
     9901 cgatgacctg accggtgact atctctcctg tacgtattgg tttggttgat tcttcctctt
     9961 cctttaataa ctctgcaaaa ctctgctctg ccattctggt atgaacctcc tcaataatct
    10021 tttttggggt ggatgcccca gctgtaatac ctacgctgcg cacagaagat acactgtcag
    10081 gatctaaatc gccgagtgtc tgaatgtaaa aggtgttctt acattccttg agacagattt
    10141 cgtaaagctt ctgggtgttg gaagaatgcc tgccgccaat aacgatcatg gcgtccaccc
    10201 gagaggcgat cttcgctgcc tccacttgtc tttcctgtgt tgcattgcaa atcgtattta
    10261 aaacactact atcataaccc ttttttaaaa ttttttcaac taaatcttga aatttgttgt
    10321 aattaaatgt cgtctgagac actacgcaca gctttttccc ctccggaacc acataatttt
    10381 gcgcttccgc ctcattctca atcactgtgg tgtatgtatt gccccagcct cttataccct
    10441 ccacctccgg atgaaaagga cttcctatga taacaaccgc atccccagcc gccgcatgct
    10501 catctacaat gcggtggatc ttctttacaa agggacaggt ggcgtctgcg atccggatcc
    10561 cgcgctcttt taaaatatca tagatatggc ggctgacgcc gtgggaacgg ataataactg
    10621 tcccctttcc ctcaggaaga gcttttaaat cctcctctgt gtccagaacg gttacgccct
    10681 tttcctctaa gtcccgtacc acttcttcat tgtggataat gggaccgtag gtataaatgg
    10741 ggccttctgc ctttccctct ttcactgcct taatctgttc atatacctga tttaccgccc
    10801 gctctacacc aaagcaaaac ccggccgtct ttgccacggt gaccgtacgg ccctccggaa
    10861 tttcaaaaga agcagcccct tttacctgcg cattcatctg tcctccccct tatttccaag
    10921 agcactgaca gctgcgctgc agcccttctc tgccgcaata acaaagatag cccgtaccac
    10981 ctcatccaga gtcatatctg agctgtcaag caaaaccgca tcctctgcct gcataagcgg
    11041 agaatgctct ctgtgcatat cctgataatc ccggctttcg atatcccgct caatctccct
    11101 taaatcagcg tcaatgccct tctctgtcag ctccttataa cgtctctcgg cccgtacctt
    11161 tacagatgca gtcagataaa tcttagccgg agcatcggga agcacacagg ttccgatatc
    11221 ccttccgtcc ataacaacat tttcccgctt tgcaagctcc ttttgaagct ccaccagctt
    11281 ttctctcaca ggaagatagc cagaggtggc ggaagccgtc tttcccacct cctcggcgcg
    11341 gatcaggccg gaaacatttt ccccatttaa aagaacctgc tggacgccgt ctttataggc
    11401 gatcgttaca tttacatttc tgcaggctgc gctgactgcc gcctcatcct gaggatcgat
    11461 gccttctctt aaaatatgca gcgctatggt gcggtacatg gccccggtgt caacgtatac
    11521 aaaacccagg cgttttgcag ccagctttgc gatggtactt tttcctgctc ccgcaggtcc
    11581 gtcaattgca atattaaagt gttccattgt ttcctcctgt tattataggg ttgtgtcctt
    11641 agccgcagcc tttcccgcct gccagcccgt agaccaggca atctgcagat taaaacctcc
    11701 ggttactgcg tcaagatcca gaagctctcc tgcaaaatac agacccttta cccgttttga
    11761 ctccatggtg gaaggatccg tctccttcac gctgatgcct ccctgggtaa taatagcctc
    11821 cttaaacgga cgaagaccgg tgagcgtaaa ggtaagtccc tttgtagctt ccaccagcct
    11881 gcgccgttcc tgtctggtga tctcattgac ctgcttatcc gggtcaatac cgctgcggcg
    11941 gacgatctcc ggaatcagct ttgcaggata aagatgactt aagctgtttt taaactgcct
    12001 gtttttcgct tctgcaaaat ccctcaggat acgctcatcc agctgttctc ctgaaagagc
    12061 cggttttaaa tcgataacca gctttaaggc cccctttgac accgcctttg cacagaagga
    12121 gctggcgctt aagatcacgg ggccgctgac tccgaaatgc gtaaacagca tctcgccaaa
    12181 ttcccggtaa agctcctttt tcccgttata gacagcaatt tcaatatttt taagagaaag
    12241 cccctgaaga gcctttacct cctctttctc cgccgcttca aagggaacca gggccggaag
    12301 aagatccgtc actttatggc ccagcatttt agcccatgca tgtccgtccc ctgtggatcc
    12361 ggtagacggg taggaaacac ctcccgtagc caggatcaca cgatccgccg gcacctcttt
    12421 ttcctttccg tccggccctt ccagacagat cccgcaacag acctgccgga ccgctccggt
    12481 atccgtatcc tcttcgcttc gcactgccag ccccttaatc tcctgcctta agtatacctt
    12541 tacacctgcc tggcgcatca tccgctccag agcggcaatc acatcggaag aatgatcgga
    12601 gcaggggaac acacggtttc cgcgctcgat tttaagaggc agccccgcct cctcaaacag
    12661 ctccatcacg tcctcattgg taaagccgta aaagctgctg aacaaaaact tgcggtttgt
    12721 cacaacggct ccaaaaagtt cttccatatc acaggcattt gtcacattgc agcgtccctt
    12781 tcctgtaata aacagcttct ttcccagctt ttcatttttt tcatacaggc tgactttaca
    12841 gccctctctt gcagcggcta ctgcggcaat cattcctgcc gcacctccgc ctgcgatgat
    12901 aatatgcttt tctttattca tgaatcctct cttgttctct gccgcggcat atattctgtg
    12961 ttgaacgtaa gtataccaca tttttccagc aaataaaacg gcccgggaca aaaaataccg
    13021 cccgggctgt tttcactgta taaatcccgg ccgatcccta cagatctgcc agtacaaaaa
    13081 tatcatagat ggcccgaatt gctttctcaa agtcatcatt cttaacgccg atgataatat
    13141 tccactcact ggaaccctga tcgatcatct ttacattcac tcttgcatga gccagggcgg
    13201 agaacacacg gcctgctgtc ccgcgattag agcgcatgcc tcttccaacc acagcgatca
    13261 gagccagatc ggattccagc tccaccacat ccggctcaac agcccggtgg attcctgcga
    13321 tcacagactg ctcatgatcc tcaaactcag actgatgaac aaaaattgtc atcgtatcga
    13381 taccggaagg catatgctca aaagaaatac ctgacttttc aaacacgctt aagacctttc
    13441 tcccaaagcc cacctctgta ttcatcatgg ccttttcaat gttgatggcg cagaagccct
    13501 tctttcccgc tacgcctgtg atcgtgtggc gcggcttgcg gcaggtcgtt tctacgatca
    13561 gcgttccctt atcctccgga cggttggtat tgcggatatt aatggggatc ccctcttttc
    13621 ttacagggaa gatcgcatcc tcatgaagaa cgcttgctcc catataggaa agctcccgga
    13681 gttctctgta tgtaatggtc tcgattacct ccggatcctc tacaatcctt gggtctgtca
    13741 ccagaaagcc ggatacatcc gtccagttct catacagatc tgcatgaacc gcttttgcca
    13801 caatggagcc cgtcacatcg gaaccgcctc tggaaaaggt ctttatgctc ccatccggac
    13861 gggaaccgta aaagcccgga accacagccc gttccacatg ggacagacgc tcagaaagct
    13921 ctttatcggt cagatctgcc tcaaagtttc cctgctcatc aaagaaaatc acctctgcag
    13981 cgtcaataaa ttcatatccc agataatttg ctatcacaat accgtttaaa tactctcctc
    14041 tggaagcagc ataatccctg cctgccccgg caagaaaatt ctcctccagt tttgcaaact
    14101 catgatccag attcaggttt aaatccaggc cctcgataat ctcctcatac cggcctcgga
    14161 ttttctccag gatcttttta taggaacgcc cctcagatgc tgcatcatag cacgcataga
    14221 gcatatccgt taccttttcg tccttgtcat tgcgttttcc cggtgcggag ggaaccacat
    14281 acctccttga cttatctgcc cggatgatat ctcccacctt tttaaactgc tttgcactgg
    14341 ccagtgaact gccgccgaat ttagacactt tcttcataat cgtagatctc ctcttgccac
    14401 attttcgaag gtaatgcttt ctggaaatga tatcccactt ctataaaaaa gtcaatagtt
    14461 tttactcaga aactttgtgt actgagcaca tttgtccgcg tgataaagac tttttattcg
    14521 cagtacaagt ggatcccctg tgactcaata aggcttctcc actcatcaga aagctcgtca
    14581 tctgtaatga tactgtcaaa atccttcaga tgagaaaagg ctgttggaaa aatccttcca
    14641 aacttggaag aatccgccag aagtattttt gtctgtccgc tttccatcag ggctctcttt
    14701 aaatccatct catggggggc aatgcaggta acattcatct tttcactgat gccggctgcc
    14761 gtaatgaagg tcttattgat gcatgttcgg tttatcatgg aaattccctc cggacagtaa
    14821 aaggtctttg tattggaata aagataaccg ccggaacaga tgatatcgca gttgtaatgg
    14881 tcatttaagt ccagaagaac attaaaactg gaagccagga ccgtcatatg gatatcatag
    14941 ggcagagccc tggcaagata ggcggccgta gatccgctgt caatggcaac tacgtcattt
    15001 gcttcaataa gagaagccgc ccgtcttcca atcctctcct ttttctgcgg aaataccagg
    15061 cactcatcat ttaaattgta ctccgggtac agagccgttt tttctgccgc atgatatatg
    15121 gcgactccgt ttaccagccg cagacagccc tgtttttcca ggagctttaa atcccttcgg
    15181 acggtaatct ccgacacatc cagcttccct gcaaactccc tgatagtaat cccgttttcc
    15241 gtcatcagca tttttctcat ccggtccaac cggtctgttt tcctgccgct catcctgttt
    15301 tcctcctttt tctaatctgt atttttttgt attatactga agaattttta tgctgctgat
    15361 ataaaatgat caaaacattt aattccctct gctataatga ttcaatccat cataaatata
    15421 acattttttc atattttttg atagcatttt atcattttat caattttatt ttgcaatatt
    15481 gacaatgagt atgataagaa ttacaatatt tttatacaac attttaccat ttttcaaaca
    15541 gagaggagta caaaatggaa ctgcgacttg tagacctggt caaggatttc tccgggacaa
    15601 aggccgttga caccggcagc ttttcaatct cagacggaaa actcaccgga cttttaggcc
    15661 ccagcggctg cggcaaatct acccttcttt acctcatctc cggtttatta aaacccacct
    15721 ccggaaggat ctttttcgga gaccgggatg tgacagagct tcctccggaa aaaagaggaa
    15781 tcggccttgt ttttcagaat tatgcccttt atccccacat gacagttgcg cagaatatcg
    15841 cctttcctct ggtaaaccgc aaggaaaaca gagagctcat ccagcaaaaa acgcagcaga
    15901 tggcaaagct ggtgcaggta gaggagcttt tgtccagaaa gccgggacag ctctcaggag
    15961 gccagcagca gagggttgcc atcgcacggg ctttaattaa agaaccggac atccttcttt
    16021 tagatgagcc tctctccaat ctggacgcac gcctcagact ggaaatgcgg gaggaaatcc
    16081 gccgcatcca gcttgaaacg ggagttacca ctattttcgt tacccatgac caggaggaag
    16141 ccatgagcat caccgacgag atcgtactga tgaaaaaggg cgtcatccag cagcagtgtg
    16201 ctcccattga tatgtactta cagcctgtaa accgtttcgt ggcatccttt atgggaaatc
    16261 ctcccattga ctttttccat tttcccataa aagacggacg cctgatcctc tcagaaagct
    16321 atgcacttcc catccgcaca accgccagcg gaaccgtact tacaggaatc cgtccggaag
    16381 cctgggagcc cggaccggga atacgggtaa caattgaaac cttggaacag agaggccgtg
    16441 atgtactggc cggttttacc ctggcaggcg caaaaggcaa ggcgctctta agcattacag
    16501 accgggtccg caccggcgat acggtcgaac tctctttaaa gcgcaaggat cttttgtacc
    16561 tgtttaatga agcaaacgaa actcggatcg atgcagaggt ggcctatgaa agttaaaggt
    16621 ttttgcagaa agccccgtcc tgaaaatccg ctgggaaata cagaatacag cctgaaggct
    16681 tacttttatc tgcttcctgc agctcttatt ttagctgtat ttgtattttt ccctattata
    16741 atgaccctgc ggatgggctt ttatgaaagc tatgtctatc tgaccgatgt aggaaaaggc
    16801 tttggtcttg cttcttttcg ctatgtgtta aaggatccag cctttcggtc tgcggtacgc
    16861 aacacactga tcatcacctt tatcggcgtt cctattacga tcgtcctgtc gctgggaagc
    16921 gccctgttta tcaactctct gggcaaagga aagggactgt ttcagacgat ctacttcctt
    16981 ccctatgtaa cctccaccat tgccatcgga ctggtgttcc ggtggctgtt ccactcagac
    17041 tatggctaca tcaaccatgt tttaagcttt tttggcctcc cgcctcagaa atggcttaca
    17101 gatcccaatc tgactaccat ttccgtcacg atctttacga tctggaacgg aatggccttt
    17161 aagatcgttc tgtttctggc aggacttcaa aagatcgatc ctcagtatta tcaggcggca
    17221 cgcatggacg aaactccgcc ttcccgtgtg tttttccgaa tcaccatgcc tcttttatcc
    17281 tctaccttct ggatggtaac cattgtatcg gtcattcacg cctttaagac ctacaatgaa
    17341 gtttactcca tgttcggagg aaacggggcc ggccccggaa acagtgccat taccattgtt
    17401 tactatatct atgatatgtt tttcaaccgt tctcaggtgc actacgcatc agccgccgct
    17461 attatctttt tcatcattat actggcgctg actgccctgc agaaatgggt atcgaaaaaa
    17521 ttcgttcact atgtataaga aaggagacgc ctcatgacag aaaaactgaa aaaagcagcc
    17581 gtctggatcc ttttaacagc cggagcactg gttatgatgt ttccttttat ctggatgtta
    17641 gccacctcct taaagacgct tccggaagcc actgccattc cgcctacgct ctttcctgca
    17701 aaggctgtct ttgcaaatta tgcggaagct ttcaccatgg cgccttttgg acggtacctt
    17761 tttaactcca tcctggtagc cgggctgggg acgctcctta ctctggtcat caccgtatta
    17821 gccgcctatg cctttacggt ttttgagttt cccggaaaat ccagcctctt cttctgctgt
    17881 cttgccacca tgatgatacc ggcagagctt ttgatcatcc agaattttat caccgtttcc
    17941 aagctccact gggtcaatac cttccagggt atcatcatac cgaccattgc cagtggattc
    18001 tatatctaca tgatgcggga atactttctt caaacccctt ctgtgcttta taaggccgcc
    18061 aaaatcgacg gctgtacgga ctggcgctat ctgtggcgca ttatgatgcc tattaacaaa
    18121 aatgccattg ccaccatcgg catcctgacc tttatcagcc agtggaattc ctttatctgg
    18181 cctctgatgg tatccaaaga tgacgcccac cgcgtcatgg caatcggtct tttacatttt
    18241 aaagatgccg tcagctccca gatcaacctg cagatggccg gttccaccat tgtccttttg
    18301 cctatgctga ttttttatct gatcttccgc aaacagatca ttgagggagt atcccgcgga
    18361 ggaatcaaag gataatattt atttcaagtc aaccaaccaa ggaggaaaac aaatgaaaaa
    18421 catgacctgt cttcttttag caggaaccgc tgcactgtct cttgctgcct gcggctctga
    18481 gtccgcgcct tcccagacat ctgatgggat tgtcacaaaa atcaccaagc ctgtagagct
    18541 tcagttctgg cattctattt ccaatcctgt ccatgcagag gttttagata agctgatcgc
    18601 ccagtttaat gataccgtcg gaaaggaaaa gcagatcact gtaaccgcta cctttaacgg
    18661 aagcagctcc gacttatatt ccaatgtaat cgctgccatt aaggcagaaa ctgccccgga
    18721 tgtaactctg gctctgcgcc cctatgtggc cgattatctt cagaccgacc tagtcgtaga
    18781 tcttaccccc tacatccagg atgaaactgt gggaatgcct gactacgaag atatatttga
    18841 aggtttgaga aacggcggaa ccacctatgc caaggaagga acgtattctc ttcccatcca
    18901 ctcctactca gaagtgcttt attacaacac cgcctttttt gaggaaaacg gcttaacggt
    18961 tcccgccacc tgggacgagc tgatcgaaac cagcaaaaaa atccatgaga tcaccggtgc
    19021 tcctgccttt ggctgggaca atctggccgg aagctttatg acccttgtaa agcagtacgg
    19081 cggacggtat acagatccgg aaggaaatat ttactttgca gaagaggatt ctgaaacggc
    19141 tttaaaggta ttaaacctct ggaaggaaaa tgtaggatcc ggtatctgga ggactgcggg
    19201 tgaggatatg tttttctccg ggccctttgc aaacgaacag atccccatgt atgtaggaga
    19261 cagcgtagaa gccagctata ttcctgccaa aaatccggaa cttaagtggt caaccgctcc
    19321 catcccacag gtaagcgatg atacggcagc caacttaagc gccggtcatg tcatcacggc
    19381 cttaaaccag tccggaaatc ccgaaaaagc ctatgcttcc tatgagctga tcaagttttt
    19441 aacctccaaa gaagccaatc tggctgtggt aaccgcaggc accggctatc ttcccatccg
    19501 ccagtctgtg gctgacagcg aagaatataa agcttacgtt gccgatggac atgattttct
    19561 taccgccgca gtagagcaga atgaccgcta tttctacgag ccggccttta ccaacgatac
    19621 cactacctcc agtgccgtta ccagcgccgt gcgtactatg atgcaggagg ttgcagaaaa
    19681 cggtacagac ccgcagactg ctctggacaa tttaaaacag accgtaggac tgaattgatt
    19741 ttccgtcctt ctgtcagaaa gacgaggaac aaatttatgg agattctcta tcatcatatt
    19801 cttactccct gcggagaacc ggcagatatt atcatctcag ctctgcagaa attcatccgc
    19861 aggggaaaaa cggaggaagc ggcccgtgct gcctatgagc tctatctgtc cggcgaggag
    19921 cttcgcgact atctgtggca gaggctttta gtcatcagcg cagaggacgt ggggcttgga
    19981 cagccggctg caccgtcggt tgtgctttct ctgtaccacg ccagccaaaa gcttggagca
    20041 gacagcatgg attaccctct gttttttgtc catgcagtcc gctatctgtg cagctgtccc
    20101 aaggaacggg gttccagcac tctggccagt ctgaccaagc ggcggatccg cgaccgcgtc
    20161 cccttccccc ttccggacta tgtatttgac atgcatacca tcgaaggaca gaatcgtggc
    20221 cgctcctatg aacattttat ctctcaggcg gctgtcgttt tcccggaagc cgttttaaat
    20281 gacagtacag ctggtcagaa tgaccgctgg gaagcagaac ttacagaaag acggaaagga
    20341 gaggaagaca tatgaaccgg acacttgtca tttccatcga tgcattgatc agcgctgata
    20401 tcgaacgttt aaaaaaactt cccaatctgg ggcgtatcat gaagcaggcc tcctgggcag
    20461 aggatatcct gtgctgctac cccactctta cctatccctg ccatgtaacc atttccaccg
    20521 gctgctggcc ggaccgccac ggtatctgca acaatgaaaa gttccagccc ctgtcaaaaa
    20581 accggccgga atggtactgg ttcagaaagg atatcaaaac gcctactctc attgactatg
    20641 caaaagcagc cgggctgacc actgcagcca ttacctggcc tgtaatgggt gattgcgggg
    20701 cagactacaa cattgcggaa atctgggctc cccatgaaga ggatgacccc actccttact
    20761 ttgacttagc agacagccca aaggccaaag agatttttga gcgcaacaag catatgctcc
    20821 gctggatgaa aacgccccag atggacgaat ttgccgccca gtgtgccgta gatattataa
    20881 aagaataccg gcctgacctt atgttcctgc atttatccta tgtggatcat cagcgtcaca
    20941 aactgggggt acgaagcgaa cagctggatc atgcctttgc ttttgtagat gaacagcttg
    21001 gcagggtatt tgacgccctg gatgaaacag gaggccttga gaataccaat atcgccgtcc
    21061 ttggggatca cggccagctc tactgcgacc agatgttcca cttaaatacc gtgctgcgcg
    21121 atatgggctt tctgacagag gaaaacggaa ccgtgacctc ctggaagata tatgccgccc
    21181 agtgcgcctt cagcgcacag atctaccgcc agccggatgt agaccctaaa ctggtctatg
    21241 aaaccctttg taaaatccgc gaccagtatc ccggaagtat ttcccagatt tttacaaaag
    21301 aacaggcggc agacctgcat ctgaccggag aatttgactt tgtagtagaa gccggggatc
    21361 ggacagcctt tgataagaca gccaatgcca ctgagtactt tacttctgtg gctgaaatcc
    21421 gcgaatacaa gctttctgtt tctacccacg gccatctgcc ggaaaaaggg gacaaacctc
    21481 cctttatctt aagcggcccg gatgtaatcc ccggaaaggt gcaaaaaggc ggatacctgg
    21541 tggatgaagc acctacgatc ctgcgtcttt taggtattac agaaaaccat atggacggaa
    21601 ccccgtttac ctggatgaca aggctgtaac aagaaggagg aaccctatga caccacgctt
    21661 catctgtttt gaccttgacg gaacccttta tctggattcc tgcgtatacc tgcggatgat
    21721 taatcatttt tttcatgaca cgccttacag agactggatt tttccggttc aggagcagat
    21781 gaaacaggta ctctccggcc agagttcctt acgatgcggg cagtttgttc ccaagcagaa
    21841 ggcagagcat ccaaaaactc ctgaagatct ttttgatata ccgggcacgg cagcccttct
    21901 tctgcctgat ccctccccct ggctggatcg aacgctttat tcctacattt ccgacggctg
    21961 gaccctgggt atgtatctgg ctcgcagaat cggctgggaa ggagaagact tctggaagcg
    22021 gttccggctg gtgcggaacg atctgatcga tccatactgg gggcctgtac cgaatccggc
    22081 tcttcctgtc cttcttaagg aattaaaaaa gcagggaatc catgtggttc tctgctccaa
    22141 cgcccaggag gctaactgta ttgagctttt agattacctt ggctttgatg actcctgttt
    22201 tgaagagctg tgctttgacg cagacaaacc ccataccttt ccccagagaa ttgcacagtg
    22261 gggcagacag tttaatctct ctccaaagga aatgcttttt atcggtgacc agggctattt
    22321 tgacctgtat tccggcaaaa catgcggcgc ttcaaccctc cttgtttctc catggaatgc
    22381 agcggatgca gaattgtggg atcacatggt acagacgcca aaggaaatgg aggcatattt
    22441 acgggagctt tgtctgaaat aggggactgt ttttaaatct ggtccgtatt tctctttctg
    22501 tacggcagat tttacggtat tcctctgctc caagccccag atcatgaacc gtttccttca
    22561 gctccccttc attgagccga agccatgagc cgttttttgt acgcacggcc tccaaaaata
    22621 atttttcaaa agacagcgtg aacccatccg ctgtcttttc tgctttcagc ccacggatca
    22681 ccataaaatg ctcccggaca accagcccct ccgatcccat gacctgaaac acgcaccagt
    22741 tttctccctt gggaaaaagc attcggccac ccggctgatc cagtttccag aaaatctgcg
    22801 gagcattttg ttcaaaacag tcgttttcct ctttcataca cattaaagga aatgacagat
    22861 gctccctttc ccttttaata tctgccccgt acccccaaaa acggtctgaa tccgttccat
    22921 ctgtatccca taactcattt ttccagattt catatccatg atacacggtt cctgtaggaa
    22981 atccctgttc aggatataca aagcggcagc ttcctctgct tcctctctta tccgtatcct
    23041 cctgaacccg tctgcgcttt gtcagttccc agcgccttcc aaggacacca tccagtttct
    23101 caaaaagccg ctcagacctg gcatccccct gatccagcct tataagtccg ccaaacatag
    23161 ccagctccac tgcattcccc tgataatgca ctttttcccc atttcttcgg aatacccgct
    23221 ctgcctgatc caaaaccgtc tcatacgcct ctgatgatgc cagaagcaca aacttatcgc
    23281 ctgcaatacg gaacaaatga tctccctctc tcatttctcc ttctattctc tgcaggcaat
    23341 ccagcacaaa acgctcccct ccttctccgc caaatacggt tttcagatag cggattcccg
    23401 gcgcctccat atcaaagcac acaatccagg tgtgctccct gtgttccagc atctttctca
    23461 tatcctccag acagaatctc cgtctgaata cgccgcagtc atcacagaaa aaatcctgtc
    23521 tgggaaaaag ctgccagaac cggcgtcttt ttctgaatct ggccggtgtt tcctgccaca
    23581 ttttcttaaa tgctcttgta aatacctcct gggatgcaaa gccatattcc agggcaatct
    23641 cgcagacgct ttgtgaggtg gataagagtt cccctgctgc ctgagtcagc ttccgtctgg
    23701 ctgcatactc catcatccca tactggaata ctttcttaaa aagagctttt gtgttggatc
    23761 tggaataata gcaggctgac gcgatctcct ctgccgaaag agattctctt atatgcgctt
    23821 ctatataatc aattacattt tttaatccct gttcttcatt catagctgcc ctccgggctt
    23881 tcatacaggc tccgcaccat ctcatccagc cctgcttttt cctttctgca gacctgtcgt
    23941 ccaatctggt aaaagtcttc cagcggttca tccagcatct tctttccgtg atcgatcaga
    24001 atcacccggc tgcagatttc atctatatcc atcatatcat ggctggtcaa gagaatagtt
    24061 gtctgcttct cccggttcag ctcctttaaa aatttccgga tcctgtcctt cgccaccaca
    24121 tccagtccta ctgtgggttc atctaaaaac agcacttccg gatcatggag cagagacgcc
    24181 gccagctctg cccgcatctt ctgccccagg ctcaggcttc gcaccggttt tcttaagaat
    24241 tcctccattc ccagcagctc tgtaaagcat tctacattac gtttatagat tgactccggg
    24301 attcggtaca tctttcggta caattcaaag gtatcctcca tgggaagctc ccagtttaac
    24361 tgagatctct ggccgaaaac cacccccatg tgaagggcat tttcctttct ctccttccag
    24421 ggaacacggc cgccgcacag aacgcttcct ccatcaggaa ccaaaatccc tccaagcatt
    24481 ttaatcgtag tggatttccc tgccccgttt ggcccgatat atcccaccat ttcccctttt
    24541 tggatggaaa atgaaattcc atccactgcc cgtttctctg tataggttct gtggatcagc
    24601 gaccgaaggg ctgcccctgc gccttttgga cgctcataca ccttaaatgt tttctgcaga
    24661 ttttcagcct caatatatgc cattctattt tcccctcttt ctgttttaat tatgagcctg
    24721 ccccgctgta ggcccgcaga cccagccgcc agaacagcct ggaaagcaaa aacacaccgc
    24781 agcctacaag gggtgtcaga taccgcagaa ttcccggaca gatcccctcc tggcggtcaa
    24841 tgaaatactg tacgggatag taattgataa atgcatatgg gagcacaaag gtcagcagct
    24901 cccggatccc ccggtgataa acagacaacg gatactggat aaattccgtc agatttttat
    24961 agagaaggcg atacaagccc tcgctctccc caatccagaa ggcaggaacc gctgtaaata
    25021 caaagcccgc cgcctgtatc aaagatgctc ccaaaagatc cgctgccagc cagaacagat
    25081 gaacgggcgt cagggcaata tccagccgga tcaggcagat cacaagcaca gctccgccca
    25141 taagataatt gcttgtatag ccggcgctta catgggaagc tgccagatat atcatcgggt
    25201 cagccggctt tgtcagcaca agatctagtt ctccctggcg gatcagccgg tgcagttttc
    25261 caaatgatcc cataaaaaag gtcgctgcaa ccgagtagga caaaatatca aaggcataaa
    25321 gaagaaggat ttcttcccgg ttccagcccc cgatctgtcc gaaccgttcc aaaaggatca
    25381 gcaccataat cattcccgta gaccagccca taatctttgc aagcatccgc atggcatatt
    25441 gtcccctgtg ggccatctgt gtcagaaaac catttttaag aaacgctccg tatactccca
    25501 ttttctcatc ctccctctat ctcaagcacc gttccgatcc ggttccaaag ccatttttca
    25561 agcaccgtta ccgccaaaat ccagataagc tccaaaatga gca
//
LOCUS       DBMDUH010000018        25450 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_1325_length_25450_cov_5.943272, whole genome shotgun sequence.
ACCESSION   DBMDUH010000018 DBMDUH010000000
VERSION     DBMDUH010000018.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 25450)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 25450)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25450
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1325_length_25450_cov_5.943272"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="ACZPCG_03020"
     CDS             <1..546
                     /locus_tag="ACZPCG_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1284076.1"
                     /translation="PVLLSALVAGITINWMFGQEYGLINFILQKMGIDPLQWSLNPIL
                     ATVVISVASNWISAGYYMLLYVGGINNINRELYEAASVDGAKKIQAFTNITLPLLMPT
                     TFIVLLLSTVSLLKEYALVQGISLGGPGSSTTYMVQYIFDQGFNQYKYGYASAAGILI
                     SLLFIVIAVIQFKATQGGGEV"
     gene            546..1382
                     /locus_tag="ACZPCG_03025"
     CDS             546..1382
                     /locus_tag="ACZPCG_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1284077.1"
                     /translation="MEGSAKRKVETICMTVLGLAVAGLMVFPIIWLGFSSFKPSSELF
                     AYPLHLFPQAPTGESYISVVADGFFNYVKNSLFLAVVGTVITVVISAMCGYALAIYRK
                     EIPYVNKVFAVFLLGTLIPGEILTIAQFTVVSEMGLYNNIWGCILPVVTTTTGIFMYR
                     QHYMSIPLELAESARLDGASEFRIFRSIMLPLGSSVTITLTIFSFMWRWNDYILPLMV
                     LSDQENFTIQIAIKSYIGTMGVDWNSILAASILSILPIIVIFIILQRYITGGLAASGV
                     KG"
     gene            1398..3041
                     /locus_tag="ACZPCG_03030"
     CDS             1398..3041
                     /locus_tag="ACZPCG_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006782264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284078.1"
                     /translation="MERYMAHLLKGAEYRTRQFMGSQVKNPASLQYGGIKGDIWEAKP
                     TIYALASALAVYFHEDSCFYKSKELYQAVDLALDFIARTQREDGSFDYPSCNFKSAAD
                     TSFCFKRLIAAYRLLVKYGNPADEAITVLKEKYLTIMHKALDAIREGGFHTPNHRWGI
                     AAALLQGSNLFAAEKEFAAGLKNRAEQYLAEGIDGDEDGEYAERSTGNYNAVVNNAMM
                     AMYQETGDEAFLGYVERNLSMMLTYMDPDDTIFTQNSTRQDQGKLEYADKYFYQYLYM
                     CSREKNPAFDGAAHKIIKDNMERGDLAPDCLHIVMNHDEMMGYHFEEYGFLEEYRKFF
                     RHAGVLRVRKPSYTYTVLNGKSAFLYFKSKGTTLCVKIGESCCDIRNFIPQTIEELPD
                     GCRLEAEAQSWYYLPFKEKQDTSDWWKMDQSKREKLISSRLKTTLEIREKEQGLELNL
                     KAEGLDRLPLRLEICIPAGVSIEHPAFALEAGAGESMILKDGYLEIHDGLTTVEIGPG
                     FGEHSFKGHYSGEEINSAGYTVYANGYTPLEKHFSLMVK"
     gene            3081..4043
                     /locus_tag="ACZPCG_03035"
     CDS             3081..4043
                     /locus_tag="ACZPCG_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR1284079.1"
                     /translation="MLRIGIDLGGTKIAAGLVDENRVLGPSIQEPTGLPRPAGELAEA
                     IYHLAYRLLKEQGLTFQDVESVGIGIPGTVNKETDCIEYANNFGFENVPFLKMLKELF
                     PCPVYGENDAKAAAWGEYLAGAGRGSRSMTAVTLGTGVGGGIILNGKILEGHNGAAGE
                     LGHMVIHQNGRPCTCGRRGCLEAYASATALVSRAKEAMEHCETSMLWNLCGRNLSEVN
                     GRIIFEASKKGDPTACEVLDSFTEDLSDGIANIINILQPEVLCIGGGLSGAGEALLAP
                     VREKTAPKIYSRASEKNTRIVLAELGNGAGIIGAACLNEQMSKN"
     regulatory      4135..4344
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            4385..5419
                     /locus_tag="ACZPCG_03040"
     CDS             4385..5419
                     /locus_tag="ACZPCG_03040"
                     /inference="COORDINATES: protein motif:HMM:NF013648.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1284080.1"
                     /translation="MKQKKLVKTVILVLSAAMIGGCGAQGSNQGDSQAVQETSAAVSE
                     SQSSSDGAYEPVTIVNGEREITFTKMPQKVLCANLYAAENMVMLGLGDRVVGRIAPAN
                     PAERPLPELEDEFAAIPEVEKSHENAVALGTDLVIGQVSAFKESAWGSYDQLESKGIQ
                     CLTITGTIVPDETVEHIYTDIENLGKIFKVEDRAEELIEDIRSQIEETQKAVSSVPEN
                     EKPKVFVLDTFKGNEIYTTSKGLQSNLIELAGGINCTRNMDEARWFNTSVETLVETNP
                     DWIIINDYGAQTVDEKKEFLDTNPALSEIPAVKNKKYLVIPLVEVMQDVRAAKACRTF
                     AETFHPECFQ"
     gene            5470..6486
                     /locus_tag="ACZPCG_03045"
     CDS             5470..6486
                     /locus_tag="ACZPCG_03045"
                     /inference="COORDINATES: protein motif:HMM:NF013219.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HIR1284081.1"
                     /translation="MVLLGSVLWTIHIGSTEIPIKEVFEICMDQFFYGGEGLKAGKWS
                     VPHFQIVWNIRLPRVLFGLLCGAGLSVCGAAMQALVLNPVGDPYILGISSGASAGAAC
                     ALLLPISLLSGPFQTTAAAFLGALLSAGTVYFMAKTAGGGMIRPVPLILSGTAVNAVM
                     SAVTSFVIFLAKSPESIAAVYNWQMGSIAAAQWKNLALPAAGVLSGTVIFTVCSRYFD
                     MMMMGDEDAAALGLSVKHFRSLMFGFCSIVVASLVSVTGIIGFVGLVVPHMARILSGT
                     STNRVIIPLSALGGGIYLIWADALARSLFGAAELPLGIVTAFAGAPFFLYLMIRNGYG
                     GGNT"
     gene            6483..7277
                     /locus_tag="ACZPCG_03050"
     CDS             6483..7277
                     /locus_tag="ACZPCG_03050"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1284082.1"
                     /translation="MILRFDGIHVRIGGKEIVKGADLCTEEGRVTGIIGPNGCGKSTL
                     IKTAMGLIKPAEGKVFIGEWEAASLSHRKLASLIGYVGQEAGCVFDFSVWDVVSMAMY
                     ARSENRDRKRPAESKTAQTVVSEALDELGILHLKDRSILSLSGGERKMVFIARAIAQG
                     AETLILDEPTNHLDICHQLFLMEYLRQCKRTVLIVLHDLRLASHYCQKVCLMKDGRIL
                     EAGDPLNVLNSARTREVFGIEGEAHMTANGERDFRLFDGSAPLIRR"
     gene            7381..8970
                     /locus_tag="ACZPCG_03055"
     CDS             7381..8970
                     /locus_tag="ACZPCG_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013658210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HIR1284083.1"
                     /translation="MNELLLLTGIVIMLCILTDRFTDRLGIPSLLAFIALGMCFGVDG
                     IFKIPFDDYEASEMICSVSLVFIMFYGGFGTNIKEAKPVIARSVVLSTAGVVLTAGLT
                     GLFIHFVLGLSLLESLLTASVIGSTDAASVFGILRSKNLNLKEHTASLLEMESGSNDP
                     ISYMLTVILTGLLTGASIRVPAMLFQQLFFGLIFGVLFGKGAVWALNRLQFQIAEGKT
                     IFVFAAAVLAYAVPVLLGGNGYLSVYLCGIIMGNSSMPHKRNMVQFFDALTGIAQMMI
                     FFLLGLLVTPSRLGVVLLPSAVIMVFLTLVGRPLAVTALLGGFKASWGQIGIVSWAGL
                     RGVASIVFAIYAVLRGVPMKYDLFNLVFCIVLLSISIQGSLLPWVSRRLNMIDEKADV
                     RRTFTDYQEEGSISFVKFHLDKSHPFIGKTLKEIVLPPELLVVLIIRGKELLVPNGGT
                     ILEQGDLLIAAAEEFEDRRNMTLQEVTVDKNYKWNGKYLHEITIPGGTLIVMIRRGTS
                     TIIPAGDTKVCEGDVLVLAKF"
     gene            9329..10495
                     /locus_tag="ACZPCG_03060"
     CDS             9329..10495
                     /locus_tag="ACZPCG_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007715285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR1284084.1"
                     /translation="MVSTGGSQTPVVAVIPLEKQDIQEKLSISGPVSGTDSVDVVSNI
                     HAEILQMNVKEGDKVIKGQVLAVLDRTELEREVNIARNAYEQAVANKEAQDKEAALGY
                     EKAVQDFQKASTDHDRNSQLFAAGDISQADMEQSANALNDARRAMEKYTVENGRGVSD
                     RSYGLQVENAAYELEKKQEELEKTEIKSEIDGTVVRVNSKVGQFADKVEDDKPIFSIE
                     NLDQLELEVKISEFSIGKVKEGQKALIGADILNGKKEEGEVIKISPTGEEKGGGSTER
                     VIPTIIRVNTPDTKLMAGITARAELLVRESKDAFAVPVSAVFDSGEASYIEIVENGKI
                     CWIPVTIGADGETKVEVLPADGTVLQEGMAVVTSPDPGLTEGMEVMVSGSQEGR"
     gene            10498..11265
                     /locus_tag="ACZPCG_03065"
     CDS             10498..11265
                     /locus_tag="ACZPCG_03065"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1284085.1"
                     /translation="MKYEERESAQRPLIQLENLVKVYDTGALKVLGLKKINLTIHRGE
                     FVAVMGHSGSGKSTLMNILGCLDRPTLGHYYLDGVDTADMTPDQLSEIRNRKIGFVFQ
                     SFNLISRTSALKNVELPMTYARIPRAARIRRARELLVRVGLEWRMDHFPNELSGGQRQ
                     RVAIARALANEPPLILADEPTGNLDTASSIEIMELFTKLYQEGATVVLVTHEEDIAAF
                     ARRIICFGDGKILSDRINHPVWEKGDGHVSGKHDDGF"
     gene            11234..12445
                     /locus_tag="ACZPCG_03070"
     CDS             11234..12445
                     /locus_tag="ACZPCG_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1284086.1"
                     /translation="MLAENMMMAFNAIRANKLRSVLTMLGIIIGIGSVIAIVSIGDTM
                     RGMFAELYKDVGITQAYVGIGYWVEDVRDSDYFNLDEMERAKEAFSDEIAYIDSNAYT
                     TADAEYKRTSISFDFQGIDSNYKEVQPVNMVYGRYLNEGDVLGRRKNVVMDTDSAMLL
                     FGTENAVGKTFRTTIYGNTDEYTVVGIYRKEVNAFQALMMGGTKDTGSAFLPYTLLTW
                     PNDRFYMLHVFAEEDVDLNRFFEEFKTYTAGLHGREPEDMYIQTAMDEMNSVDSVMGG
                     LSAAVGGIAAISLLVGGIGIMNIMLVSVTERTREIGIRKALGARTRDVLIQFLTESAI
                     LSACGGIAGVILGTGVVALGGAVLGLKTVIKPSVILIAVTFSAVVGIFFGLYPALKAA
                     KADPIDALRYE"
     gene            12504..13655
                     /locus_tag="ACZPCG_03075"
     CDS             12504..13655
                     /locus_tag="ACZPCG_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR1284087.1"
                     /translation="MKIIGLTFLFGFAAAQTVLASPEFAYPPEKWASLRDDVLEYEEL
                     ADLIHEYNAEVINSRLEYDDYRGKSNDDFRNTYSEMADRLEDSSDRMMDGINEDEPGY
                     GAAEASAMSIRLQAELARDQADMENEDGRVKKLEYDRQEALLIQKAQGLMIEYWKKEK
                     ERPVLEGTLQQAEAEVQTARRKAALGMLSQPELLEAEAAVCTAQAAEASGKTKQEELR
                     RQLIVMTGWQYDDLPRLGEIPIPEEALLEEDPESDRTQALAANFSLAADRRRLENTDS
                     GTAKDVLNQKVEAGQQKIEADIEAKYNSLMQAQTDYGQAKEEYRLAEEKLEADERRYQ
                     LGLLSANDRMAAQAELSSKKAAWEISGLSFRQAYEDYQWAVEGLAAYEG"
     gene            13674..14804
                     /locus_tag="ACZPCG_03080"
     CDS             13674..14804
                     /locus_tag="ACZPCG_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284088.1"
                     /translation="MKKEAGRRTVIRVMAVLFVGIMAGTVAASGVSRGEETEMALCYE
                     NLENLVKESCPQVEMERRQFEERLGRLEDAREELLESRRALREEASDREKDGDAAGAE
                     HYRWQAEALLDGADSLKEQIRQAGNAAGRMELRRMEDTMLWTAQNLMGTYHSLEAEQA
                     AASARAEAQKHLSEKVKDQEMLGSSTSYHSQEAYQKWQSALNEYERLKKEQERVRQEL
                     LLLTGFSPDSPVVLEKMPLPDVSRAALVNPQEDRQQALGNNYELRSGRMGNAGSNQEL
                     HKKQRQTELDENQMFASLETLGDQIRSCQAEWEAACAKEQAQRAVWQSAVRKREQGTM
                     AEGEYLEIYSQYMEEKGNQAQASIRFLLAMEEYDWAKKGLMD"
     gene            15143..>25450
                     /locus_tag="ACZPCG_03085"
     CDS             15143..>25450
                     /locus_tag="ACZPCG_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family protein"
                     /protein_id="HIR1284089.1"
                     /translation="MNKRHWRRFCALFLAAAILITGIPFQSAAKENTVEMIIRGDAFQ
                     NRPINIFGHSHRSITCYYVNPVEGHVPAFCLQPGKKLPNHTVSSYTRYDAEPGASVPV
                     IGSFDRYLPMTLAYEWMVSGNYYDKTRYAMVQTYLWGCLAGYEEAWDVQEEMMHKLES
                     VIRDGKVLPFFHEMREYVENGLAEYENQGNSDLPEWNGRQQRMELKDGRYELTLDISS
                     CPQLKNAVWQFPDGNWIYEIGADGTSVTFIYQGETPSGRISSGPLSGIKTKYYAYIFQ
                     PEEKYQMQMGWLDMADTDAEVWFEAGPSLDSQEGPGLELYRHKEVFESNYKVQVEKYC
                     AETGKPLEEAEFNVWETFDFGQVNEHGYEEGNPDGSTGEVYVNCMSPKPEERILCDVI
                     STDENGTASHSDIRSYHYSKTYCMGHPAPEWVECDHEGEGEGDEAEECSCEEENERLR
                     SQWLAEQELCSRTCDFHVGNEDEDNHSQSTKAMEAMLEDRDETYENFIRLEYGYTAEE
                     KKARNGYTIHGTHRDDPMIETVVLMSAQAEGNVRQGEMEVSGEMGLPALKTYGESLEA
                     LGEERGYRCLLPEGEKMDLEELRSILEIREQERTEPAKKESAGETTGVQETQSASGQE
                     QAGTQEPDKEGLESRPDSQQTGGEAGNKEEAGGQSGWNETEGEGVENPSEDEKMEGTE
                     AGNPSDKQETGGEVNGNSPEGQESSGESSGNPSDKQDNVGEEAGNQSGGQEVGGEVSE
                     NPPDSQKTGGQEAGNQPGSQESGSVAEKRDPDIAEIAISPHLRSRLLSPFSSAPIHFL
                     ATSNDAQREEELPEKGEQEEGLLPEEEDMPVTEQFEYIKHPVAPFLLSEPEYTSAPRE
                     KIREDQKKHPFSLKALFSFLSEDEEEGDSLYAVLPDFIDDDLEPMDPSDYGDPLRCLY
                     RFKIWDHRTEGRIHINKRDLELYKGNPEGSYGKTQGNATLEGAVYGLFAAQDIIHPDG
                     KSGVVYGRDQLTAIAATDKEGNASFLAYTECPGTLWNGSGTEVSAFTGPENLYQGSQI
                     TSSDHGFGTKIYPDYKSENGSVWIGRPLLMGSYYVMELSRSEGYELSVQGLNKAETNR
                     EADESVTVISSGSAWVKNGLSDHNSMEADGSWNDFTIESYDAENGYDITITGYPEHTW
                     FYRLSSEERRENVKQIVNTVPQQKVDEHGTPVYKKAAGGELKTDEHGNPILKEDSSEE
                     EKLPLKETLPYRFRTAPYPFGTAEPEDLSLWDQPVEEDYLKEQAEAMLAQLGYHLTAE
                     EMGAPWVNIPLKKENSFLDNSTAASQILDWYTEHGFYDCAGVGAVYEKEGEWFARLHY
                     DHSGNIPPMSGIYDRADQRMYVKKEAEQGIYWLEYEKGTYSLKSRTVTLKEKRELEGE
                     VLLEENTLDHAAVCCQPVYECYKEGEILLDVQENPIPEMEQVPVYEEKEVVYQTLAKE
                     PVEAVWDASAGSYTIHFIPKSVKGQNVQDVFRAVTDKKTIELHGTAIPYNQYLIHEMG
                     AAVDVASSIIKQDPGSYIKPVLLSYPGQNQPVQDGETQKEPVQVLERAIKQSVRVSKD
                     ISQESYENVNTYGSVHNDPMTALLGLFGGSGRGTKLLDQFKFRLYLKSDLVNLYADEA
                     GNLLSEDISSKDFTEAVQAHFLPPKDGGGQKLLEKKADGSWNYKKFFDALYAADQRPG
                     TEAAEQKLTEFALSYVDVKAYKEEILKQQPDLNEDRAYRQALAQAKEEAKAYTERFKG
                     LDEKLSIAWDRDPSGGADRDKKTLQCSTRNGKDDYFDTSIMLPYGEYVMVEQQPSALE
                     GELANRHYRIEEPKEITLPFVPEIVENEQTGEENVLDETGSSYFRYNSTDSAEELVRK
                     YKIRFNGESHVIRANGQDGEFEVYKYGLDLNSRPGHSLTSSQPYEEEYMDGANPAVKA
                     YYSGYTSESENKGIMDGVVYDGNETENGQVQIRDQVPVMEGEGTAIHGKFAPMLVPWT
                     VLAPATDRVNPDTGSIETLYPSGSGANFNYVSFAQEDVENTYFSSRLRIEKMDAETGE
                     TIFHDGALFRIYAAKRDVKKTGTGTAGGSGQVLFGSAVDAEGKPVKDENGKQILYPRV
                     GADNSSESDVPIRLDEDGIPLYDESQRISQLDENGIKKGIFRSYSTLKELVIDGRLCE
                     VPVGYIETARPLGAGAYVLVEVQAPKGYTKSRPVAFEIYGDQVVYYEENTLKEGNGWE
                     RKTARQYEYAIPVTGEENRRGTEKVSEVPVQDYPSELWIYKTEDGDSLVGNGNGLKKT
                     DAQGKEEASGGFTGNQEEIYVNDRGDLLLYKVSGRKEKLEARGDVREITFDQEKQIWT
                     GYVTKAFDSYSEHVIEGTEQELKAMDRVKLLYELDGTFTGKGIRFDRPVSGAELVLYE
                     GSCLEPKKNGGYEGVTVLSREGRTEKIVNTHTGTHRELLKSGTDLGPGKLDLWEDQKQ
                     PNEPIELYFYDLEKEKSQGRLREDIHTGELQVLDERGNVICFADKASGMAYVRDDYGR
                     IIAYRADEKGEKIPVRSICVGENGGAPSIYKDKQSADDENGLPVYYKNGEPVWKEERW
                     VTGESTGPDGKEEGMDEAHVIARLPFGAYILQEEGVPFEQGYVQAPYQGLILEDRKGP
                     QKYFHQDEFTKAAFAKIDIRTQKEIKGAEMTLYRALLDKNGRPETEENGIYKKGEPWA
                     AWISGYAYDDKGVMKKDSQGNPIPTDEPHWIDHIPVGFYVLEETLCPYDQGYVQSESV
                     NVEIQETGHVQSFEMEDDFTAIEVWKLDGKRDEVLCGDTETGLTLYRALLDAEGRPVM
                     EEGHPAIREDLAVFTFRAATWEDGQKVAATGRVETDAAGLYPIMKYDYQYNDIPGTFQ
                     GRYYYTENASVRMEYLPVGSYVLAETQTPEGYATADPILIEIPDKGHMVSVGQWSMKD
                     EPLKLSVSKRVISGGKEVAGAELSIYPVSEDGRISSEALILHIPEKDGSCREGEARWI
                     SGNDGKYTQKEAEAGTIPEDFEEGDLKPHLIEYIPDGDYILREEMTPYGFLQSVDLPF
                     SVIDSRVIQKLEMKDEIPDGVLQVIKHDGDDHELLLEGAEFELFNKTLGISCQKAVTD
                     FRGRAVFEKQPAGYLASNGSFAPYIYVCREIKAAPGHMLSAPDFEFQFEYKDERTKIL
                     NVQHDPENDSNRVKVEKQLGDTGERLKGALLRLERKISAEDGGHTGKIRWETAAEWIS
                     GDQPHLEKGLKEGEYRLTEQKAPEGCNVLAEPVYFTIADGMKEALQLVMKNYTVIVQI
                     AKTDRSSGAFLAGARLQLIRTDTGEVIEEWTSEEHTPKTFVGLKPGSYRIHELYAPES
                     YERAEDMEIEIEDQSAEIQTFSFANTRITSSGGGGSHRPQKEYISFKKTDVNHLPVPG
                     AEFTFYDQWGNEMDRAVSDEKGYFSIPKPSDGTYTFRETKAPQGYGLDPVLHSFTVSQ
                     GEIYRGDYEITDREMKVIIRKLDGDTKE"
BASE COUNT         7094 a         5005 c         7353 g         5998 t
ORIGIN      
        1 ccggttcttt tatcggctct ggtagcaggt attacaatta actggatgtt cggacaggaa
       61 tacggtctga tcaattttat tttacagaaa atgggaatcg atcctcttca gtggtcttta
      121 aatcccattt tggcaacagt ggtgatcagt gtggcatcca actggatttc agcaggatac
      181 tatatgcttt tatatgtagg cggtatcaat aatatcaacc gggagctgta tgaggcagca
      241 agtgtagacg gtgcaaagaa gatccaggca tttaccaata tcacacttcc ccttctgatg
      301 cccactactt ttatcgtact ccttctttct accgtatctc ttttaaagga atacgcgctg
      361 gtacagggta tttctcttgg aggacccggc tcctcgacta catatatggt acagtacatt
      421 tttgatcagg gctttaatca gtataagtac ggatacgctt ccgcagcagg cattcttatc
      481 agccttctgt ttatcgtgat cgcagtgata cagtttaaag caacccaagg cggaggtgaa
      541 gtataatgga aggatcagca aagcgcaaag tagaaaccat ctgtatgacc gttttaggac
      601 tggcagtagc aggactcatg gttttcccga tcatctggct gggattcagc tcatttaagc
      661 cttcttccga gctgtttgcc tatcccttac atcttttccc gcaggctcct accggtgaat
      721 cctacatcag cgtagtggca gacggatttt ttaactatgt aaaaaatagt ctctttctgg
      781 cagtggtagg taccgtgatc actgttgtga tcagtgccat gtgcggctat gctcttgcca
      841 tctaccgcaa ggagatcccc tatgtgaata aggtatttgc cgtattcctg ttaggaacct
      901 tgatccccgg agagatcctg accatcgcac agtttaccgt agtcagtgaa atggggcttt
      961 acaataatat ctggggatgc atccttccgg ttgtaaccac cacaacaggt attttcatgt
     1021 acagacagca ctatatgtcc attcccttgg agcttgcaga gtctgcaaga ctggacggcg
     1081 cttccgagtt tcggattttc cgctccatca tgcttcccct gggatcttca gttaccatta
     1141 cattgacgat cttctccttt atgtggcgtt ggaatgatta tattctgcct ctgatggtgc
     1201 tttccgatca ggaaaatttt accatccaga tcgcgatcaa gagctatatc ggaaccatgg
     1261 gtgtggactg gaactccatt ttggcagctt ctattttatc tatccttcct attatcgtta
     1321 ttttcattat cttacagcgt tatatcaccg gcggcctggc tgccagcggt gtaaagggct
     1381 aaaaacggag gcttataatg gaacgttata tggcgcatct gctgaaggga gcagaataca
     1441 gaacaagaca gtttatggga agtcaggtga aaaatcctgc ctccctgcag tacggaggga
     1501 tcaaaggaga tatctgggag gcaaagccga cgatctatgc cctggcttct gccctggctg
     1561 tgtatttcca tgaggacagc tgcttttata agagtaagga gctgtatcag gcagtggatc
     1621 tggcccttga ttttattgcc agaacccaga gagaggacgg aagctttgac tatccctcct
     1681 gcaactttaa atcagcagca gatacttcct tctgtttcaa acggctgatt gcagcctacc
     1741 gtcttctggt aaaatatggg aatcccgctg atgaggctat tacagtttta aaggaaaagt
     1801 atttaaccat tatgcataag gctctggatg caatccgtga ggggggcttt catacgccga
     1861 accatcgatg ggggatcgca gcggctcttc ttcagggctc caatctcttt gccgcagaaa
     1921 aagagtttgc cgcagggctt aaaaaccggg cagaacagta tctggcagag ggcattgacg
     1981 gagacgagga cggagagtat gcggagcggt ccacgggaaa ttataatgca gtggtaaaca
     2041 atgccatgat ggccatgtat caggaaacag gtgacgaggc atttttaggc tatgtggagc
     2101 gcaacctttc catgatgctg acctacatgg atccggacga taccattttt acccagaatt
     2161 ctaccagaca ggatcaggga aagctggaat atgcggataa atatttttac cagtatctct
     2221 atatgtgcag cagagagaaa aatcctgcct ttgacggggc tgcccacaag attataaagg
     2281 acaatatgga gcgcggagat ctggctcccg actgtctgca tattgtgatg aaccatgatg
     2341 aaatgatggg atatcatttt gaggaatacg gatttttgga ggaataccgc aagtttttcc
     2401 gccatgcagg cgtactccgg gtaagaaagc cctcttatac ttatactgtg ttaaacggaa
     2461 agagtgcatt tttgtatttc aaatccaaag gaaccacact ctgcgtaaaa attggagaga
     2521 gctgctgtga catccgcaat ttcattcctc agaccataga ggagctgccc gatggctgcc
     2581 gcctggaggc ggaggcacag agctggtatt atctgccctt taaggaaaag caggatacct
     2641 ctgactggtg gaaaatggat cagagcaagc gggaaaagct tatcagcagc cggttaaaga
     2701 caactcttga gatccgggaa aaggagcagg gtctggagct taacttaaag gcagagggcc
     2761 tggatcgtct tcccttacgg ctggaaatct gtattccggc aggcgtttcc atcgagcatc
     2821 cggcctttgc actggaggca ggtgccggag agagcatgat attaaaggat gggtatctgg
     2881 aaattcatga cggtttaaca accgtggaga tcggccccgg ttttggagag cactctttta
     2941 aggggcatta ttcaggagaa gagattaaca gcgccggtta tacggtgtat gcaaacggct
     3001 atacccctct tgaaaagcat ttctccctta tggtaaaata aaccagatca taatctatga
     3061 atacataaag gggaaacaat atgctgagaa tcggaattga tcttggggga acaaaaatcg
     3121 ctgccggact ggtagatgaa aacagagtgc tggggccgtc cattcaggaa cccacgggcc
     3181 tgccaaggcc tgccggagag ctggcagagg ctatttacca tctggcatac cggcttttaa
     3241 aggaacaggg acttaccttt caggatgtgg aaagtgtggg gatcggcatt ccgggaacgg
     3301 taaataagga aacggactgt atcgagtatg ccaataactt tggttttgaa aacgttcctt
     3361 ttcttaaaat gttaaaggag cttttcccat gcccggttta tggggaaaat gacgccaagg
     3421 cagccgcctg gggcgaatat ctggccggtg cgggacgggg aagccgttcc atgacagccg
     3481 taaccctggg aaccggagtc ggcggaggca ttattttaaa tggaaagatt ttagaaggcc
     3541 acaatggggc agccggagag ctgggccata tggtgatcca tcagaatgga cgcccctgta
     3601 cctgcgggcg gagaggctgt ctggaggcct atgcttctgc taccgctctg gtgagccgtg
     3661 caaaggaggc aatggagcat tgtgaaacat ctatgctttg gaacctttgc ggaagaaatc
     3721 tctcagaagt aaatggcagg atcatctttg aggcttctaa aaaaggagat ccgacggcct
     3781 gtgaggtatt ggacagtttt acagaggatt tatcggacgg aattgccaat atcatcaata
     3841 tccttcagcc ggaggtgctt tgcatcggcg gaggcttaag cggagccggg gaagccctcc
     3901 ttgctcctgt aagggagaaa acagctccga agatttattc cagagcttca gagaaaaaca
     3961 cccgtatcgt gttagcagag cttggaaatg gggcaggcat cataggagcg gcctgcttaa
     4021 atgagcagat gagcaaaaac taaggaaaaa ttttgacata tgatgaaaag ctgatataat
     4081 agctttgacc cgtacacggc cctgtttaca taccgtttta aggttgtaaa cagggctgct
     4141 gttatatatg gcagtgcctg aaaaggcctg acagggaagc cggacctaga agaagcagag
     4201 ccgacagctt cttaaaaaac ggcgcagccc ccgctactgt actgaaggac gaagtcttta
     4261 gatatgtcac tggcataaaa gctgggaagg caaagacgga ggatgaattt aagccaggag
     4321 acctgctgcc atgagaggat gcataaaatg catacgggat catatacttt cggagggaaa
     4381 gaacatgaaa cagaaaaaac tggtaaaaac agtgatcctt gtcctgagtg ctgccatgat
     4441 aggcggctgt ggtgcacagg gaagcaatca gggtgattct caggcagtac aggaaacgag
     4501 tgcagccgtt tcagaatccc agagcagctc tgacggagct tatgagcctg taaccattgt
     4561 aaatggagaa cgggaaatca catttacaaa gatgccgcaa aaggtacttt gtgcgaatct
     4621 ttatgcagca gaaaatatgg ttatgctggg gttgggagac cgtgtagttg gacgtattgc
     4681 tccggcgaat ccggcagagc gccctcttcc agagttggag gacgaatttg ctgcgattcc
     4741 ggaggtggag aaatcccatg aaaatgcagt ggctttagga acggatcttg tgattggtca
     4801 ggtaagtgct tttaaggaat ctgcctgggg aagctatgac cagttagaaa gcaagggaat
     4861 ccagtgtctt acaattacag gtacgattgt accggatgaa acggtagaac atatttatac
     4921 agatatagaa aatctgggaa aaatatttaa ggtagaggac cgggcggaag aactgataga
     4981 ggatatccgt tctcagattg aagagacaca aaaagcagtt tcttctgtac cggagaatga
     5041 aaaaccgaag gtatttgttc ttgatacatt taagggaaat gaaatttaca caacctcaaa
     5101 gggactgcag agcaacctga ttgagctggc gggagggatc aactgtaccc gtaatatgga
     5161 tgaagccaga tggttcaata caagcgtgga aacgctggtg gaaacgaatc ccgactggat
     5221 tatcatcaat gattatggcg ctcagacggt agatgagaag aaagaatttt tagatacgaa
     5281 tccggcttta tctgagattc cggcagtaaa aaataaaaag tatcttgtga ttccgctggt
     5341 ggaagtaatg caggatgtac gggctgccaa agcctgccgt acatttgctg aaaccttcca
     5401 tccggaatgt ttccagtagg acggcaggga aagaggggga gacagcgcct cctctttccc
     5461 gtgctgacaa tggttctttt aggatctgtt ctgtggacga ttcatatcgg atctacagag
     5521 attccgataa aggaagtttt tgagatctgt atggatcagt ttttttacgg aggagaaggt
     5581 ttaaaggcag gaaaatggtc cgtacctcat tttcagattg tatggaatat ccggctgcct
     5641 agagttctgt tcggtcttct ttgcggagca ggtctttctg tctgcggagc ggctatgcag
     5701 gctctggtgc tgaatcctgt tggcgatcct tatattctgg ggatttcatc aggagcatct
     5761 gcaggagctg cctgtgcgct tttgcttccg atttcactac tttccggtcc ctttcagacg
     5821 actgcggcgg cttttttggg cgcgctttta tctgccggca ctgtttattt tatggcgaaa
     5881 acggcagggg gaggaatgat acggcctgtg ccccttattt tgtcgggaac ggctgtcaat
     5941 gcggtgatga gcgcagtaac cagttttgtt atttttctgg caaaaagccc ggagagtatt
     6001 gctgctgtat ataactggca gatgggaagc atcgcggcgg cacagtggaa aaatcttgct
     6061 cttccggcag ctggcgtttt atctggaaca gtgatattta ctgtttgcag ccgttatttt
     6121 gatatgatga tgatgggaga tgaggatgcg gccgctctgg gattatctgt aaagcatttc
     6181 cgttctctga tgtttgggtt ctgctcgatt gtagtggcat cgctggtgtc tgttacggga
     6241 attatcggtt ttgtgggact cgtggtacct catatggccc gtattctttc cggaaccagc
     6301 acgaaccggg tgattatacc tttaagtgct ctgggaggag ggatttatct gatctgggca
     6361 gatgctctgg ccagaagtct ttttggcgct gctgaactgc cccttggtat tgtaacagct
     6421 tttgcaggag cgcctttctt tttatacctt atgatacgaa atggatatgg gggtggaaat
     6481 acatgatact tcgttttgat ggaatccatg tgcgtattgg tggaaaagag attgtaaaag
     6541 gggcagatct gtgtacagag gaaggaaggg tcacaggaat tataggaccc aacggatgcg
     6601 gaaagtccac cctgataaaa acagcgatgg gactgataaa gccagcggag ggtaaggttt
     6661 ttatcggaga atgggaagcg gcatctcttt ctcacaggaa actggcgtct ctgatcggct
     6721 atgtaggtca ggaagcaggc tgcgtatttg atttttctgt gtgggatgtg gtatctatgg
     6781 caatgtatgc cagatctgaa aacagagaca gaaagcggcc ggcagaatcg aaaactgcac
     6841 agacagtggt ttcagaggca ttagatgagc tgggaattct gcatttaaag gacagaagta
     6901 tcctttcgct gtctggcggg gagcggaaaa tggtatttat tgccagagcc attgcacagg
     6961 gagcagagac gctgatcctg gatgaaccga ccaatcatct ggatatctgt catcagctgt
     7021 ttcttatgga atacctcaga cagtgcaaaa ggacggttct gatagtactt catgatctgc
     7081 gtctggcctc ccattattgt cagaaggtat gtctgatgaa ggatgggaga attctggagg
     7141 ctggcgatcc tctgaatgtg ctgaattctg ctcggacccg tgaggtcttt ggaatagaag
     7201 gagaagccca tatgacagcg aacggtgaga gggatttcag gctgtttgat gggagtgctc
     7261 cgttaataag gcgttaaaat tgcagtaagg ttcctttttg accgctggaa catttgacgg
     7321 ctttttttcc ggctgttata ataaaaccca ttatactgct gggaaaggat tctgatttct
     7381 atgaatgaac ttttgcttct cacgggaatt gtgatcatgc tctgtatcct gacggaccgg
     7441 tttacggatc gtcttggcat accgtccctt ctggcattta ttgcccttgg catgtgcttt
     7501 ggagtggacg gtatttttaa aattcctttt gatgattatg aggcatcaga gatgatctgt
     7561 tccgtcagcc tggtgttcat tatgttttac ggaggctttg gaaccaacat taaggaggca
     7621 aagccggtga ttgcccggtc tgtggtattg tctaccgcag gcgtagttct tactgcaggg
     7681 cttacgggtc tttttattca tttcgtttta gggctttctc tgctggaaag ccttttgacc
     7741 gcatctgtca tcggctccac ggatgcagct tcggtgttcg gcatcctgcg ttctaaaaat
     7801 ctgaacttaa aggagcatac ggcctcttta ctggaaatgg aatccggttc caatgatcct
     7861 atttcctata tgctcacagt gatcttgaca gggcttctca cgggcgcgtc catccgtgtt
     7921 ccggctatgc tgtttcagca gctttttttc ggtctgattt ttggggttct cttcggaaag
     7981 ggagcggtct gggccttaaa ccgcctgcag tttcagattg cagagggaaa gaccattttt
     8041 gtatttgccg cggctgttct tgcctatgcc gttcctgtgc ttttgggagg aaacgggtat
     8101 ttaagcgtgt atctttgcgg aatcattatg ggaaattctt ccatgcctca taaaaggaat
     8161 atggtacaat tttttgatgc gcttacagga attgcacaga tgatgatctt ctttttgctg
     8221 gggctgttag tgactccatc cagactgggc gtggtgttgc tgccttcggc agtgatcatg
     8281 gtatttctga cattagtcgg acgtcctctg gctgtgacag cgcttctggg cggttttaaa
     8341 gcatcctggg gtcagatcgg aattgtttcc tgggccgggc ttcggggcgt ggcatccatt
     8401 gtatttgcca tttacgctgt gctgcgcgga gtaccgatga aatacgacct gtttaatctg
     8461 gtattttgta ttgttttgtt atccatttcc attcagggaa gcctgctgcc atgggtttcc
     8521 aggcgtctga acatgataga tgaaaaggcg gatgtgcgcc gcacttttac ggattatcag
     8581 gaagagggca gcatcagttt tgtgaaattc catctggata aaagccaccc ctttatagga
     8641 aagacgttaa aggagatcgt tcttcctccg gaactcttag tagttttgat tatccgcggg
     8701 aaagaacttc tggttccaaa cggaggtact atattggagc agggggatct tctgattgcc
     8761 gcggcggagg aatttgaaga ccggagaaat atgacccttc aggaagtaac ggtggataaa
     8821 aattataaat ggaacggaaa atatcttcac gaaattacaa ttcctggggg gacgctgatc
     8881 gttatgatcc gaaggggcac ttctaccatt atcccggcag gagatacaaa ggtctgcgag
     8941 ggagatgtgc ttgtgttggc aaagttttga ggcaggatat cctgcctttt tgcgtgttta
     9001 gaaattcttt cattttcatt tagaaagaat tggaagttat cagaggtaaa tattcgggag
     9061 gctttggtat gatatgagac aggaacggca gtgaaaagga cagggctgcc gacagagcgg
     9121 gtgaggagga gttatggaac aaagagacga agaaatgggg agacgggaag aaaacagggg
     9181 gccggagcgg gcatctgaaa gtgaggaaat tgaccgcaca ctggaacaga taatggacaa
     9241 tcagaaacag gagaaaaaga aacggcccgg aaagaaaaaa tggatcctgg cagcggggat
     9301 tctcggtttg gggattttag gtataaaaat ggtttccacg ggaggcagcc agactcccgt
     9361 ggtagctgtg attcctctgg aaaagcagga tatacaggaa aagctttcca tcagcgggcc
     9421 tgtatccggt acggacagcg tggatgttgt gtcgaatatc catgcagaga ttcttcagat
     9481 gaacgtaaag gaaggggata aggttataaa ggggcaggtt ctggccgtgc ttgaccgcac
     9541 agagctggaa cgggaggtaa atattgcccg caatgcctat gagcaggcag tggccaataa
     9601 ggaagcccag gataaggaag cggcgcttgg atatgagaag gcggtgcagg attttcaaaa
     9661 agcaagtacg gatcatgaca gaaacagcca gctgtttgca gccggagaca tttcacaggc
     9721 agatatggag cagagcgcaa atgccttaaa tgacgccaga cgggcgatgg agaagtatac
     9781 ggtggaaaac ggccggggcg tttcggatcg ttcctacggc cttcaggtgg aaaatgcggc
     9841 ttatgagctg gaaaagaaac aggaggagct ggaaaagaca gaaattaaaa gtgagattga
     9901 cggtacagta gttcgggtca attccaaggt aggccagttt gccgacaagg tagaggatga
     9961 taagcccata ttcagcattg aaaatctgga tcagctggag ctggaggtta agatcagtga
    10021 gttttccatc ggaaaggtaa aggaaggcca gaaggcgctg attggagcgg atatattaaa
    10081 cggcaaaaaa gaggagggag aggtgataaa aatctctcct accggtgaag aaaagggagg
    10141 cggttccaca gaacgggtca ttcccacgat tatccgcgtc aatacgccgg atacaaagct
    10201 gatggcaggg attacggccc gtgcagagct gcttgtcagg gaatcaaagg acgcttttgc
    10261 agttcctgta tcagcggtat ttgacagcgg agaagcttcc tatatagaga ttgttgagaa
    10321 tggaaagatt tgctggattc ccgtaaccat tggagcggac ggggaaacaa aggtggaggt
    10381 gctcccggca gatggaacgg ttttgcagga aggaatggct gtggtaacat ctccggatcc
    10441 cggcttgacg gaggggatgg aggttatggt ttccgggagt caggagggca gataagaatg
    10501 aaatatgagg aaagagaatc tgcacaaagg cctcttatcc aactggaaaa tcttgtaaaa
    10561 gtatacgata cgggagcact taaggttctg ggattaaaaa agatcaacct gacgattcac
    10621 cggggagaat ttgtggctgt catgggacat tccggttcag gaaagtccac gctgatgaat
    10681 attttaggct gcctggaccg gcctaccctg ggacattatt atctggatgg agtggatacg
    10741 gcggacatga cgccagatca gctctctgaa atccgcaacc ggaagatcgg ctttgtgttt
    10801 cagtccttta atctgatctc ccgtacgtcg gctcttaaaa atgtagagct tcccatgacc
    10861 tatgcgcgga ttcccagagc tgcacgaatc aggcgggcca gagagctgtt agtccgggtg
    10921 ggcttagaat ggcgcatgga tcatttcccc aatgagcttt ccggaggaca gcgccagaga
    10981 gtggccattg ccagggctct tgcaaatgaa ccgccgctga tcctggcaga tgagcccaca
    11041 ggcaatctgg atacggcttc ctccatcgag attatggagc tttttacaaa gctttatcag
    11101 gagggagcta cggtggtttt ggttactcac gaggaggata ttgctgcctt tgccagacgg
    11161 atcatctgtt ttggagacgg gaaaatctta agcgaccgga tcaatcatcc ggtgtgggag
    11221 aaaggagacg gccatgttag cggaaaacat gatgatggct tttaatgcga tccgggccaa
    11281 taagctccgt tcggttctta ccatgctggg gatcattatc ggcatcggat ctgtgattgc
    11341 cattgtttcc attggagata ccatgcgggg aatgtttgct gagctctata aggatgtggg
    11401 tattactcag gcttatgtgg ggatcggata ctgggtagag gatgtaaggg acagcgatta
    11461 ttttaatctg gatgaaatgg agcgggcaaa ggaggcattt tcggatgaaa ttgcctatat
    11521 tgacagcaat gcctatacga cggcagatgc ggaatataag cgcacctcca tcagctttga
    11581 ttttcagggg attgattcca attataaaga ggtacagccg gtgaatatgg tttacggacg
    11641 gtatctcaat gagggggatg tgcttggaag aaggaaaaat gtggttatgg atacggacag
    11701 cgccatgctt ttgtttggta cggaaaatgc agtgggaaag accttcagga ccaccattta
    11761 cggcaatacg gatgaataca ccgtggtagg gatctaccgg aaggaagtga atgcatttca
    11821 ggctctgatg atgggaggaa cgaaagatac aggctctgcc tttcttccct atacccttct
    11881 tacctggccc aatgaccggt tttatatgct ccatgtattt gcagaggagg atgtggatct
    11941 gaaccgcttt tttgaagaat ttaagactta taccgcaggg cttcacggca gggagccgga
    12001 ggatatgtat atccagacag ccatggatga gatgaacagc gtggattctg ttatgggagg
    12061 gctgtctgca gcagtaggcg ggattgcggc tatttcactg ctggtaggcg gcatcggcat
    12121 tatgaatatc atgctggtgt cggtgacgga gcgcacccgt gagatcggca tccgaaaggc
    12181 cctgggagca aggacaaggg atgtgctgat ccagtttctg accgaatcgg ccatcctgtc
    12241 tgcctgcgga ggaattgccg gagtgatctt gggaacgggc gttgtggcgt tgggaggagc
    12301 ggttctgggc ttaaaaacag tgataaagcc ttccgtgatc cttattgcgg ttacattttc
    12361 agcggttgtt ggaattttct ttggattgta tccggcctta aaggctgcaa aggcagatcc
    12421 gattgacgct ctcaggtatg aataacagaa gggagagaag gaagatgaaa acacagggga
    12481 tatgctgtgg ccggaggggc tgcatgaaga tcatcggcct gacatttctc tttggctttg
    12541 ctgcggctca gactgtttta gccagccctg aatttgccta cccgccggaa aaatgggcgt
    12601 ccctgcggga tgatgtgctg gaatatgagg aactggcaga tctgatccac gaatacaatg
    12661 cagaggtgat caacagccgt ctggaatatg atgattaccg gggaaaatcc aatgacgatt
    12721 tccgcaatac gtattctgaa atggcggatc gtttagagga ctccagtgac cggatgatgg
    12781 acgggataaa tgaagatgag cccggatatg gagccgcaga ggcatcggcc atgagtatac
    12841 ggcttcaggc agagctggcc agagatcagg cagatatgga aaatgaagac ggcagggtaa
    12901 aaaaactgga gtacgacagg caggaggctc ttttaatcca gaaggcccag gggctgatga
    12961 ttgaatactg gaaaaaagaa aaggagcgtc cggttcttga gggaaccctg cagcaggcgg
    13021 aagcagaagt tcagacagcc agacggaagg cagcccttgg aatgctttca cagccggagc
    13081 ttttagaggc agaggcagcc gtctgtacgg ctcaggcagc agaagcctca gggaagacta
    13141 agcaggagga gctgcgccgc cagttgattg ttatgaccgg atggcaatat gatgatctgc
    13201 ctcgtctggg agaaattccc attcctgagg aggcattgct tgaagaagac cctgagtcag
    13261 accgaacaca ggcattagct gcgaatttca gtctggcagc agaccgccgc agactggaaa
    13321 atacagattc tggtaccgca aaggatgtgc ttaatcagaa ggtggaagcc gggcagcaga
    13381 aaatagaagc agatatagag gcgaaatata acagcctgat gcaggctcag acggattacg
    13441 gtcaagcaaa agaagaatac cgcctggcag aggaaaagct agaggctgat gaacgaagat
    13501 atcagctggg gctgttgtct gccaatgacc ggatggcggc tcaggcagag ctgtcatcta
    13561 aaaaggcagc ctgggagata agcggcctgt cttttcggca ggcatacgag gactaccaat
    13621 gggcagtcga agggctggca gcgtatgagg gttgaggaaa gcaggggatt tggatgaaaa
    13681 aagaagcggg aaggcggacg gttatcaggg tgatggctgt gctttttgtg ggaataatgg
    13741 ccggtacagt ggcagcttcc ggcgtgagca gaggggaaga aacggaaatg gcgctgtgtt
    13801 atgagaatct ggagaatctg gtaaaggagt cctgtccgca ggtggaaatg gagcgaaggc
    13861 agtttgaaga gcgtctgggc cgtctggaag atgcaaggga agaactctta gagagccgga
    13921 gggctttgag agaagaggcc tctgatcggg aaaaggacgg cgatgcggca ggagcagaac
    13981 attacagatg gcaggcggag gctcttttgg atggtgcaga cagcctgaaa gagcagatcc
    14041 gtcaggcggg aaatgccgca ggacggatgg aactgcgcag gatggaggat accatgctct
    14101 ggacggctca gaacctgatg ggaacgtatc acagtctgga agcggagcag gctgcggcca
    14161 gcgccagggc agaggcacaa aagcatttat ctgagaaagt taaagatcag gaaatgctgg
    14221 gaagcagcac ctcttaccat tcacaggagg cttatcagaa atggcagtcc gccctaaatg
    14281 aatatgaacg gcttaaaaag gagcaggagc gggtccgtca ggagcttttg ctgctcacag
    14341 gcttttcacc ggacagcccg gttgttttag aaaaaatgcc cctgcccgat gtatcaagag
    14401 ctgctcttgt gaatccacag gaggacaggc agcaggcttt gggaaataat tatgagcttc
    14461 gcagcggacg catgggaaat gcaggaagca atcaggagct tcacaaaaaa cagagacaga
    14521 cagagctgga tgaaaaccag atgttcgctt cccttgagac tctgggggat cagatccgtt
    14581 cctgtcaggc agaatgggag gcagcctgtg cgaaggaaca ggcgcaaagg gcagtgtggc
    14641 agtctgcggt cagaaaaagg gaacagggga caatggctga gggagaatat ctggagattt
    14701 acagccagta tatggaagaa aaaggaaatc aggcacaggc atccattcgt ttccttctcg
    14761 caatggagga atatgactgg gcgaaaaagg gattgatgga ttaggacaat caggaaaatc
    14821 ggttacaaaa tttctgtaaa aacaggacag gagagttgac ttgtcctgtt tcttatgcta
    14881 ttctgtaaga accgatacgg aattctctgt atcatttatc taaatttcat tttattggct
    14941 gtttcagccg catttcagaa caaaaacaga aaaattgaat cagaacagag tctggaaaga
    15001 cgaggcgtat tttgcgcacc cgtaattgcg gatcgtatat ccggggactg tatgaaaagg
    15061 gggaatgtcc atatatggcc tggtaggatt attttataaa acaaatgtaa ctgttcagga
    15121 gaaaccaagg gggacataac aagtgaataa aagacattgg aggcgttttt gcgccctttt
    15181 tctggcagcc gctattctca ttacggggat tcccttccag tcggcggcaa aggagaacac
    15241 ggtggaaatg atcatccggg gagatgcatt tcagaaccgt cccatcaata ttttcggaca
    15301 cagccaccgt tcgattacat gttactatgt aaatccggtg gaaggccatg tgccggcctt
    15361 ctgccttcag cccggaaaaa agctgcccaa tcatacggtg tcatcctata cccggtatga
    15421 cgcagagccg ggagcatctg tgcctgtcat cggaagcttt gaccggtatc ttcctatgac
    15481 attagcttat gagtggatgg tgtccgggaa ttattatgat aagacccgat atgctatggt
    15541 gcagacctat ctctgggggt gccttgcagg ctatgaggag gcctgggatg tgcaggagga
    15601 gatgatgcac aaattagaga gcgtgatccg ggatggaaag gtgctcccat ttttccatga
    15661 gatgcgggaa tatgtggaaa atggtctggc ggaatacgaa aatcagggaa attcggacct
    15721 tccggagtgg aacggacgcc agcagagaat ggagctcaag gatgggagat atgagcttac
    15781 tctggatatc agcagctgcc cccagcttaa gaatgcagtg tggcagtttc cggacgggaa
    15841 ctggatctat gaaataggag cagacggaac ttctgttacc tttatctatc agggagaaac
    15901 accttccgga cggatttctt ccgggccttt aagcggaatt aaaacaaagt attacgctta
    15961 cattttccag ccggaggaga aataccagat gcagatggga tggctggata tggcggatac
    16021 ggatgcagag gtctggtttg aagcaggccc gtctttggac tctcaggaag gtccgggact
    16081 ggaactttac aggcataaag aggtatttga gtccaattat aaggtgcagg tggaaaaata
    16141 ctgcgcagaa acaggaaagc ccttagagga ggctgagttt aacgtatggg aaacctttga
    16201 tttcggtcag gtcaatgaac acggctatga ggagggaaat ccggacggaa gcacaggaga
    16261 ggtttatgta aactgcatgt ctccaaaacc ggaggagcgt attctctgtg atgtgatttc
    16321 cacagatgaa aacggaacgg cttcccacag cgatatccgc agttatcatt acagcaaaac
    16381 ctactgtatg ggccatcctg cgccggaatg ggttgaatgc gaccatgagg gagaggggga
    16441 aggagacgag gcagaggaat gcagctgtga ggaggaaaat gaaagactgc gcagccagtg
    16501 gctggcagaa caggagcttt gcagcagaac ctgtgacttt catgtgggaa atgaggacga
    16561 agataaccat agccagagca caaaagccat ggaagcaatg ctggaggaca gggatgagac
    16621 gtatgaaaat tttatccgtc tggagtatgg gtacaccgca gaggagaaaa aggccagaaa
    16681 cgggtatacc attcacggaa cgcaccgcga tgaccccatg attgaaaccg tagttcttat
    16741 gtctgcccag gcagaaggca atgtacggca gggtgagatg gaggtttccg gtgaaatggg
    16801 gcttccagct ttaaaaacgt atggagagag cttagaagcg ctgggagagg agcggggata
    16861 ccgctgtctg ctcccggaag gggagaaaat ggatctggaa gagcttcgga gcatccttga
    16921 aatccgggaa caggaacgta cagaaccggc aaagaaggaa agtgccgggg aaacgactgg
    16981 tgttcaggag acccagagtg cgtcaggtca ggagcaggcc ggcacacagg aaccggataa
    17041 ggagggactg gaaagtcggc cagacagtca gcagactggt ggagaagccg ggaataagga
    17101 agaggcggga ggacagtcag gatggaatga gacggaagga gaaggggtcg aaaatccgtc
    17161 ggaagatgag aaaatggaag gaacagaggc cggaaatccg tcagataagc aggaaaccgg
    17221 cggagaagta aacggaaatt cgccggaagg gcaggaaagt agtggagaaa gttcgggaaa
    17281 tccgtcagac aagcaggaca atgtcggaga agaagctgga aatcaatcgg gcggtcagga
    17341 agtcggtgga gaagtctcag aaaatccgcc agacagtcag aaaactggcg gacaagaagc
    17401 cgggaaccag ccgggcagcc aggaatccgg cagcgtagca gagaaaaggg atccggatat
    17461 tgccgaaatt gccataagtc ctcatttgcg cagccgtctt ttaagtccat tttcctctgc
    17521 tccgatacac tttttggcta catccaatga tgcacaacgg gaggaagagc tgccagagaa
    17581 gggggaacag gaggaaggac tgctgccgga ggaggaagat atgccggtta cggagcagtt
    17641 tgaatacata aaacatcccg ttgccccctt ccttctatcc gaaccggaat atacctctgc
    17701 tcccagagaa aaaatcagag aggatcagaa gaaacaccca ttcagcttaa aagcactttt
    17761 ttcatttctg tcagaggatg aggaggaggg ggacagtctt tatgctgtac ttccggattt
    17821 catagatgat gatctggagc caatggatcc ttccgattat ggggatcctc ttcgctgtct
    17881 ttatcgtttc aaaatctggg atcaccggac agagggacgg attcatatca ataagcggga
    17941 tttggagctt tataaaggaa atcccgaagg aagttatgga aaaactcagg gaaatgccac
    18001 gttagaggga gcggtatacg gtctttttgc tgctcaggac attattcatc cggatgggaa
    18061 aagcggtgtc gtatatggca gggatcagct gacagccatc gctgccacag acaaggaggg
    18121 gaacgcctca tttttagcat acactgaatg tccgggtacg ttgtggaatg gcagcggtac
    18181 agaagtttct gcgtttacgg ggcctgaaaa tctgtatcag ggcagtcaga ttacctcatc
    18241 cgatcatggc tttggaacga aaatctatcc ggattataag tcagaaaacg gctcggtctg
    18301 gattggacgt ccccttctca tgggcagcta ttacgttatg gaattgagcc ggtcggaggg
    18361 atatgagctt tctgtgcagg gccttaataa ggcagagaca aaccgggagg cagatgagag
    18421 cgttacagtg atttcttccg gttcagcctg ggtgaaaaat ggcctttctg atcacaacag
    18481 tatggaagcg gacggctcct ggaatgactt taccatcgaa tcgtatgatg cagaaaatgg
    18541 ctatgatatt acaattacag gctatccgga gcatacgtgg ttttaccgtt tgagttcgga
    18601 ggagcgcagg gaaaatgtaa agcagattgt aaatacagta ccgcagcaaa aggtggatga
    18661 acatggaacg cctgtttata aaaaggctgc aggcggtgaa ctgaaaacag acgagcatgg
    18721 aaatcctata ttaaaagaag atagttcaga agaggaaaag cttcctttaa aagagacgct
    18781 tccctaccgt ttccgcacag ctccctatcc ttttggcaca gcagagccgg aggatctctc
    18841 cttatgggat cagcctgtgg aagaggatta tttaaaggag caggcagagg ccatgctggc
    18901 tcagcttggc tatcatctga cagcggaaga aatgggtgct ccctgggtca atatcccgtt
    18961 gaaaaaagaa aattcttttc tggataattc cacagcggcc agtcagatac tggactggta
    19021 tacagagcat gggttttatg actgcgccgg agtgggagcg gtctatgaaa aagagggaga
    19081 gtggtttgcc cgtctgcatt acgatcattc gggaaacatt cctcctatga gcggtattta
    19141 tgaccgggca gatcagcgga tgtatgtaaa aaaggaagcg gagcagggaa tttactggct
    19201 ggaatacgaa aagggaacct acagcctcaa aagccgtaca gtgacgctga aagaaaagag
    19261 agagcttgaa ggcgaggtac ttttagagga aaacaccctg gatcatgctg cagtctgctg
    19321 tcagccggtt tatgagtgtt ataaggaggg ggaaatcctg cttgacgttc aggaaaatcc
    19381 gattcctgag atggagcagg tgcctgttta tgaagaaaaa gaggtcgtat accagactct
    19441 ggcaaaggag cctgtggagg ccgtgtggga tgcatccgca ggcagctata cgatccattt
    19501 tattccgaaa tcggtcaaag gacagaacgt acaggatgta tttcgggcgg ttacggataa
    19561 aaagaccatc gagcttcacg gaacagcaat cccgtataat cagtatctga tccatgaaat
    19621 gggagcggct gtggatgtgg cttcttctat cataaaacag gatccgggaa gctatataaa
    19681 gccggtgctg ctgtcttatc cgggccagaa tcagccggta caggatgggg agacgcaaaa
    19741 agaaccggtt caggtgttgg aacgggcgat taaacagtct gtgagggttt ccaaagacat
    19801 ttcacaggaa tcctatgaaa atgtaaacac atacggttcg gtccacaatg atcccatgac
    19861 agcgcttttg ggacttttcg gtggttccgg aagaggaaca aagcttttgg atcagtttaa
    19921 attccggtta tatttaaaga gcgatcttgt aaatctctat gcagatgagg caggaaatct
    19981 tttgtcagag gatatcagca gcaaagattt tacagaggca gttcaggctc attttctgcc
    20041 gccaaaggat ggcggcgggc agaagctttt agagaaaaaa gcagacggaa gctggaatta
    20101 taaaaagttt tttgatgcat tgtatgcggc agaccaaagg ccgggaactg aggcagcgga
    20161 acagaagctg acagaatttg cattgtctta tgtggatgta aaagcatata aggaggagat
    20221 tttaaagcaa cagccagatc tgaacgaaga cagggcctac aggcaggctc ttgcacaggc
    20281 caaagaggag gccaaagctt atacagagcg ttttaaggga ctggatgaga aactttcgat
    20341 tgcctgggat cgggatccgt cagggggtgc agaccgggat aaaaagaccc ttcagtgcag
    20401 cacacggaat gggaaagatg attattttga tacctccatc atgctccctt acggggaata
    20461 tgtgatggtt gagcagcagc cttctgccct tgaaggtgag ctggcaaacc gtcattatcg
    20521 gattgaggaa ccaaaagaaa ttacgcttcc ttttgtgcct gaaatcgtgg aaaatgagca
    20581 gaccggggaa gaaaatgttc tggatgaaac aggaagttcc tatttccgtt ataacagtac
    20641 agattctgcc gaggaactgg ttcgtaagta taagatccgt tttaacggag aaagccatgt
    20701 gatccgggcg aacggtcagg atggggaatt tgaggtatat aaatacggtc tggatctaaa
    20761 cagccgtccc ggtcacagcc ttacttcctc acaaccttat gaagaagaat atatggatgg
    20821 agccaatccg gcggtaaagg cttattacag tggctatacc agtgaaagtg agaataaggg
    20881 aatcatggat ggggttgtct atgatggaaa tgaaacggaa aacggacagg tgcagatccg
    20941 ggatcaggtt cctgtaatgg aaggggaagg cactgcaatt cacggtaaat ttgctcccat
    21001 gcttgtaccc tggacggttc ttgcccctgc cacagaccgg gtcaatccgg atacgggaag
    21061 cattgaaacg ctttatccgt caggaagcgg agccaatttt aactatgttt cctttgctca
    21121 ggaggatgtt gaaaatacct atttcagctc caggctgcgg atcgaaaaga tggatgcaga
    21181 aacgggagaa actattttcc atgacggggc tctttttcgt atctatgctg ccaagcggga
    21241 tgtaaaaaag acaggaaccg gcacggcagg aggaagcgga caggtcctct tcggttcggc
    21301 tgtggatgca gagggaaagc cggtgaaaga tgaaaacggg aagcagattc tttacccaag
    21361 agtgggagca gacaatagtt ctgaaagtga tgttcccatc cggctggatg aggatgggat
    21421 tcccctttat gatgaaagcc agaggatcag ccagctagat gaaaatggga taaaaaaggg
    21481 aattttcaga tcctattcta ccttaaagga gctggtgatt gacggacgac tgtgtgaggt
    21541 acctgtgggc tatatcgaaa cggcgcgtcc tctgggagcg ggagcctatg tgctggtaga
    21601 ggttcaggcc ccgaagggat atacaaagag ccggcccgta gcctttgaga tttacggaga
    21661 ccaggtcgtt tactatgagg aaaacacctt aaaggaaggg aatggctggg agagaaaaac
    21721 ggccagacag tatgaatatg ccattcctgt aaccggggag gaaaaccgca gaggcactga
    21781 gaaggtaagt gaggtacctg tacaggatta cccatcagag ctttggatct acaagacgga
    21841 ggacggcgat tctctggttg gaaatgggaa cggcctaaaa aagacagatg ctcagggaaa
    21901 agaagaagca agcggaggct ttacaggaaa tcaggaggaa atttatgtaa atgacagggg
    21961 cgatcttctc ctctataaag taagcgggcg aaaggaaaag ctggaggcca gaggggatgt
    22021 tagggaaatt acctttgatc aggaaaagca aatatggacc ggctatgtaa caaaggcctt
    22081 tgactcatat tccgaacacg taatagaggg aaccgagcag gaattaaagg ctatggaccg
    22141 ggtgaagctt ctgtatgagc tggatggtac atttacggga aaaggcatcc gttttgaccg
    22201 gccggtttcc ggggcagaac tggtgcttta tgaaggaagc tgtctggagc caaagaaaaa
    22261 cggcgggtat gagggtgtta cagtcctgag ccgtgaggga aggacagaaa aaatagtaaa
    22321 tacgcataca ggtacccaca gggagctgtt aaaaagcggt acagatctgg ggccgggcaa
    22381 gctggatctc tgggaggatc aaaagcagcc caatgaaccc atagagctgt atttttacga
    22441 tttagagaag gagaagagcc agggacggct gcgggaggat atccacacag gagaactgca
    22501 ggtactggat gagagaggaa atgttatatg ctttgcagat aaagcctccg gtatggccta
    22561 tgtcagggat gattatggaa ggatcattgc atatcgggca gatgaaaagg gagaaaaaat
    22621 cccggtgcgt tcgatctgcg tgggagaaaa cggaggagcg ccttctattt ataaggacaa
    22681 gcagagcgca gatgatgaaa acggacttcc ggtatattat aaaaacggag agccggtatg
    22741 gaaagaagaa cgctgggtta caggagaaag cacaggcccg gatggaaagg aagaagggat
    22801 ggatgaagct catgtgatcg cccgccttcc ctttggagcg tacattttac aggaggaagg
    22861 tgttccgttt gagcagggat atgtccaggc tccgtatcag ggattgatcc ttgaggatag
    22921 gaaaggacct cagaaatatt tccatcagga tgaatttaca aaagctgcct ttgcaaaaat
    22981 tgatatccgc acacaaaagg agatcaaagg ggcagagatg accctttaca gagctctttt
    23041 ggataaaaat gggagaccgg aaacagaaga aaacggtatt tacaaaaagg gggagccctg
    23101 ggccgcctgg atcagcggat atgcgtatga tgataaaggc gtgatgaaaa aggattcaca
    23161 gggaaatccc atccctacgg atgaacctca ctggatcgac cacattcccg ttggatttta
    23221 tgtgctggag gaaacgcttt gtccttatga ccagggctat gtacagtcgg agtctgtcaa
    23281 tgtggaaata caggaaacag gccatgttca gagctttgaa atggaggacg attttacagc
    23341 cattgaggtt tggaaactgg atggaaagcg ggacgaggtg ctctgcggtg atacagaaac
    23401 aggacttacc ctttacagag cccttctgga tgcagaggga aggccggtga tggaagaggg
    23461 ccatccggcc attcgggagg atctggctgt atttaccttc cgggcagcta catgggagga
    23521 tggacagaaa gtggcagcta ccggacgggt ggaaacggat gcagcaggac tttatcctat
    23581 tatgaaatat gattatcagt ataatgatat ccctggaacc tttcagggaa ggtattatta
    23641 tacagagaat gcatcggtcc ggatggaata tctgccggtg ggaagctatg tactggcgga
    23701 gactcagacg ccggaagggt atgccacagc agatccaatc ctgattgaga ttcccgataa
    23761 gggtcatatg gtgtccgtag gacagtggtc tatgaaggat gagccattaa agctttctgt
    23821 gtccaaacgt gtgatttccg gcgggaaaga ggtggccgga gcggagcttt ccatttatcc
    23881 ggtatcagaa gatggaagga tatccagtga agcgctgatc ctccatatcc cggaaaagga
    23941 tgggagctgc cgtgagggag aagcaaggtg gatttcagga aatgatggaa aatatacaca
    24001 gaaagaggca gaggcgggaa ccatcccgga ggattttgaa gaaggggatt taaagccgca
    24061 tttgattgag tatattccgg acggggatta tattctcaga gaagaaatga cgccctatgg
    24121 ttttttacag tccgtagatc tgccgttttc cgttatagat tccagagtaa ttcagaaact
    24181 ggaaatgaag gatgaaatcc ccgatggggt gctgcaggtt atcaagcatg atggagacga
    24241 ccatgaactt ttactggaag gagcagaatt tgagctcttt aacaaaacgc tgggaatttc
    24301 ctgtcagaag gcagtcaccg atttcagagg aagggctgtg tttgaaaagc agcctgccgg
    24361 gtatctggcc tctaacggaa gctttgcccc ctatatctat gtgtgcagag aaataaaggc
    24421 agctcccggc catatgcttt cagctccgga ctttgaattt cagtttgaat ataaggatga
    24481 aaggacgaag atcctgaacg tgcagcatga tccggaaaac gacagtaatc gggtgaaggt
    24541 ggaaaaacag ctgggagata cgggagaacg gttaaaaggc gccctgcttc ggttagaaag
    24601 aaagatatct gcggaggacg gaggccatac ggggaaaatc cgctgggaaa cagcggcaga
    24661 gtggatcagc ggagatcagc ctcatttaga aaaggggctt aaagaaggag aataccgcct
    24721 tacagagcag aaagcgccgg agggctgcaa tgttttggca gagcctgttt attttaccat
    24781 tgcagacggt atgaaagagg ctttgcagct ggtgatgaaa aattatacag tcattgtaca
    24841 gattgccaag acggatcgta gttccggagc ttttctggca ggagccaggc tgcagctgat
    24901 ccgaacggat acaggggaag tgatagagga gtggacctct gaagagcaca ccccaaagac
    24961 ctttgtgggg cttaagccgg gaagctaccg gatccatgag ctgtatgcgc ctgagagcta
    25021 tgaaagggca gaggatatgg aaattgaaat agaggatcaa tccgctgaaa ttcagacatt
    25081 ttcctttgcg aatacgagaa tcacttcttc cggcggcgga ggcagccaca ggcctcaaaa
    25141 ggagtacatt tcctttaaaa agacagatgt taatcatttg cctgtaccag gagcggaatt
    25201 tacgttttat gaccagtggg gaaatgagat ggatcgggca gtaagtgacg aaaagggata
    25261 tttttccatc ccgaagcctt ctgatggaac ctataccttc cgggaaacaa aagcgcctca
    25321 gggctatgga ctggatccgg tacttcacag ctttacagtc agccaggggg agatataccg
    25381 gggggattat gagataacag acagggaaat gaaggtaatt ataagaaaac tggacggaga
    25441 tacaaaggaa
//
LOCUS       DBMDUH010000019        25033 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_1351_length_25033_cov_5.061327, whole genome shotgun sequence.
ACCESSION   DBMDUH010000019 DBMDUH010000000
VERSION     DBMDUH010000019.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 25033)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 25033)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25033
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1351_length_25033_cov_5.061327"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="ACZPCG_03090"
     CDS             <1..464
                     /locus_tag="ACZPCG_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HIR1284090.1"
                     /translation="NRLDVPVRHAFLSLAVHIILVEVFLMIFHMGIFSVVFANILFAF
                     MMCLLNGRSIARYAGYRQEYKKTVILPFVCSLLMGAAAWSIYKGLVFLCPALLSGGRL
                     GMAVLVFPPVAVSMAVYGVLLVKLKAIDEKELKEMPMGTRLVRLCKRVRLM"
     gene            543..950
                     /locus_tag="ACZPCG_03095"
     CDS             543..950
                     /locus_tag="ACZPCG_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284091.1"
                     /translation="MMKRMYCYALLFLGTAFLCLVLIYGLTRYRIRQEEPIPNTILET
                     ETVRDMEGREAVNQSGVLPAAKMTQEEPKEEYFLVSEAGFLLVFCSDKSTICLYTHIP
                     VTDFPERERARLMEGIWFPSMMDVYHYLESYTS"
     gene            1014..2261
                     /locus_tag="ACZPCG_03100"
     CDS             1014..2261
                     /locus_tag="ACZPCG_03100"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006780602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BaiN/RdsA family NAD(P)/FAD-dependent
                     oxidoreductase"
                     /protein_id="HIR1284092.1"
                     /translation="MKNRTVGIIGGGASGLMAAISAARAGAQVTLIEHMDRVGKKILS
                     TGNGRCNLTNYRMEPECYRCSQKDFPMKVIQGFGVEETLEFFKYLGIVPKDRNGYVYP
                     NSDQAASVLDVLRWETERLGIRVLLSCQVSVIKPQRDGGFLIRSSQGTLHTDTLILAA
                     GSKAAPSTGSDGSGYELAKALGHKVIKPLPALVQLRCQGTLYKQLAGIRTEARIRLLA
                     DGICMAEDKGELQLTDYGLSGIPVFQISRYASRALDKGSRVRASIDFMPSWKEDEAFR
                     LLKKRAVMLKEKPVGELFTGMLNRKLAQVLIKLSGIDPSALCERLGNRQLERILYQLK
                     SYEAIVMSVNPFSNAQVCCGGVDTREVDPDTMESRIRTGLYLAGELLDVDGICGGYNL
                     QFAWSSGWIAGQSAAGIEKPHRK"
     gene            2284..3927
                     /locus_tag="ACZPCG_03105"
     CDS             2284..3927
                     /locus_tag="ACZPCG_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR1284093.1"
                     /translation="MIRINQLKLPLEYEKEKLLQKAARILGISPADILKIDIIKESID
                     ARKKPEIFYSYIVDVTLKSGGSKREESIVRKKKDRNLSISHEKPYCLPECGTAPLAYP
                     PVIIGTGPAGLFCGLMLARKGYCPILLERGEDVDARTRRVEAFWKDGVLNPASNVQFG
                     EGGAGTFSDGKLNTLVKDTFGRNREVLKILSQFGADPSICYQNKPHIGTDVLSRIVKS
                     IRQEIESLGGIVRFLSQVTDFRIKDGKLYALEINGKELLYTDAAVLAVGHSARDTFET
                     LYRHSVPMEAKAFAVGLRIQHPQAMINMSQYGMEECSALGPASYKLTRQTTAGRGVYS
                     FCMCPGGYVVNASSEPGRLAVNGMSYHDRAGENANSAIIVSVTPEDFSHDNDEKNPLS
                     GIYFQRELEEKAYQAAGGNIPVQLYGDFISDCTSDHFGKVRPAFRGQYGFANLRRILP
                     EALSASFIEAMEGFGHMIKGFDCPDAILAGIESRTSSPVRIPRDETLESAVRGLFPCG
                     EGAGYAGGITSAAMDGIKVAEELIRRYRPLKEKTGELEG"
     gene            4013..4876
                     /gene="proB"
                     /locus_tag="ACZPCG_03110"
     CDS             4013..4876
                     /gene="proB"
                     /locus_tag="ACZPCG_03110"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HIR1284094.1"
                     /translation="MEAQERKNLKDKKRIVIKIGSSSLTHPETGELNLMKIEKLIRTL
                     SDLRGQGKDVVLVSSGAIAAGRQALGHHKRPDSLAEKQAFAAVGQARLMMVYQKLCAE
                     YNQTAAQVLLTKDTMVNDASRYNAQNTFEELLNLGTIPIVNENDTVSTSEIPYVDSFG
                     DNDRLSAIVAALIGADLLILLSDIDGLYTDDPRTNKEARFISFVPEITKELLSMGKGT
                     SGSDVGTGGMSAKLAAARIATDSGADMVIANGDHVEAVMEIVGGKETGTLFLAHTNLD
                     FDLMHYLNNEY"
     gene            4892..5113
                     /locus_tag="ACZPCG_03115"
     CDS             4892..5113
                     /locus_tag="ACZPCG_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HIR1284095.1"
                     /translation="MDAKKIDRINELYHKSKSVGLTEKEKSEQAALRREYLAAIRGSL
                     RNNLNNISIVEKDGSVTDLGKKYGGIKEV"
     gene            5153..6397
                     /locus_tag="ACZPCG_03120"
     CDS             5153..6397
                     /locus_tag="ACZPCG_03120"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HIR1284096.1"
                     /translation="MTLNEIGGQARAVSRILNSLGSREKNIGLEEAARALLEGEDEIL
                     AANEADYEMARQKGMSQGLLDRLRLTPARVQSMADGLLKVASLEDPVGEVISMKLRPN
                     GLKIGQKRVPLGVIGMIYEARPNVTADAFGLCFKSGNAVILKGGSDAGESNKAIVRWI
                     RTGLVRAGLPEDAVQLIEDASRETTRELMRLNQYVDVLIPRGGAGLIRTVVENSTVPV
                     IQTGTGNCHIFVDESADFHMALDIIFNAKTQRIGVCNACESLVVHRKIAADFLPLLRK
                     KLEEKQVEIRADEAARAIEPSFKPAEPEDWGTEYLDYILSLKLVDSLDEAIRHINTYN
                     TGHSEAIITSDYFNAQKFLDEVDAAAVYVNASTRFTDGEEFGFGAEIGISTQKLHARG
                     PMGLKELTTTKYIIYGDGQIRS"
     gene            6418..6996
                     /gene="lepB"
                     /locus_tag="ACZPCG_03125"
     CDS             6418..6996
                     /gene="lepB"
                     /locus_tag="ACZPCG_03125"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR1284097.1"
                     /translation="MLEEEKKGRSRKKEEEEPFDLKKEIISWIQIIAAAVIIALVLNN
                     FIIANSRVPSGSMENTIMTKSRVIGSRLSYLTDDPERGDIVIFHYPDDESVYYVKRVI
                     GLPGETVEIVDGKVYINGSDTPLEEPYLPEPMEGSYGPYTVPEGSYFMLGDNRNNSRD
                     ARYWENKFVKKEKIIAKVLFCYYPLSQFGPVE"
     gene            7094..8602
                     /gene="miaB"
                     /locus_tag="ACZPCG_03130"
     CDS             7094..8602
                     /gene="miaB"
                     /locus_tag="ACZPCG_03130"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HIR1284098.1"
                     /translation="MELSANISYEALHGAPPACEPERQQYFMEKAKKQLEVFKDRLGR
                     PLTCCINTFGCQMNARDSEKLLGILEAIGYVASDTEEADFVLYNTCTVRENANLRVYG
                     RLGQLGARKKKHPHMMIALCGCMMQEKEVVEKIRKSYRYVDLIFGTHNIFKLAELISI
                     CLEKRQEELLNEEEGVHTKHTMVVDVWKDTDMIVEDLPVERKYSFKSGVNIMFGCNNF
                     CSYCIVPYVRGRERSRKPEEIIKEIKRLVADGVVEVMLLGQNVNSYGKNLETPMTFAE
                     LLREIEKIPGLERIRFMTSHPKDLSDELIQVMAESQKICRHLHLPLQSGSSRILKEMN
                     RRYTKEQFLALAEKIKAAVPGISLTTDIIVGFPGETEEDFEETLDVVRNVRFDSAFTF
                     IYSKRTGTPAAAMENQVPEDVVKERFDRLLKEVQDISAEVCGRDVHTVQQVLVEEVND
                     HSPELMTGRMSNNTVVHFPGDASMIGSLVDVYLEESRGFYYMGRIHEDSGEA"
     gene            8635..11274
                     /gene="mutS"
                     /locus_tag="ACZPCG_03135"
     CDS             8635..11274
                     /gene="mutS"
                     /locus_tag="ACZPCG_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HIR1284099.1"
                     /translation="MMQHYMETKKEYPDCILFYRLGDFYEMFFDDALAASKALEITLT
                     GKECGLKERAPMCGVPYHAVDSYLYRLVQKGFKVAIAEQMEDPKQAKGLVKREVIRVV
                     TPGTITSAQALDETKNNYLMGIVYVDGRYGISTADISTGEFLVTEVDSDRELFDEINK
                     FSPSEIICNNAFYMSGVDLDELKNRYQVIISALDSHFFGDESCKKVLKEHFKVNSLSG
                     LGLEDYATGIIAAGAVMEYMYETQKSTLEHITSLTPYRTGQYMVIDTSTRRNLELVET
                     MREKQKRGTLLWVLDRTKTAMGARLLRSYIEQPLIHKDEIIRRQDAVEELNMNYISRE
                     ELGEYLNPIYDLERLIGRISYKTASPRDLIAFKTSLEMLPHIKRLLGEFQTGLLKELE
                     SELDPLEDIWDLIRRSIVDDPPITVRDGGIIKEGFHEEADRLRNAKTEGKTWLAELEA
                     REKEKTGIKTLKVKYNKVFGYYFEVTNSFKDMVPDYFVRKQTLTNAERFTTDELKQLE
                     DVIMGAEEKLTALEYDLFCEVRDSISSQVVRIQQTARAIAGIDVFCSLSSVATRRNYV
                     KPSINEKGVIHIKNGRHPVVEQMMRDDLFVANDTFLDNGKNRLSVITGPNMAGKSTYM
                     RQVALIVLMAQLGSFVPAQEADIGICDRIFTRVGASDDLASGQSTFMVEMTEVANILR
                     NATRNSLLVLDEIGRGTSTFDGLSIAWAVIEHISSTKLLGAKTLFATHYHELTELEGT
                     IAGVKNYCIAVKEQGDDIVFLRKIVRGGADKSYGIQVAKLAGVPDSVISRAREIAGEL
                     SENDITARAKEIAQANANVTQHRAVPKPDEVDLQQLTFFDTVKDDDIIKELDGLELST
                     MTPLDALNTLYRLQTKLKNRWKE"
     gene            11281..12204
                     /gene="trmD"
                     /locus_tag="ACZPCG_03140"
     CDS             11281..12204
                     /gene="trmD"
                     /locus_tag="ACZPCG_03140"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HIR1284100.1"
                     /translation="MNFHILTLFPDMVMDGLGTSITGRAMESGAIQAEAVDIRTYSRD
                     KHRHVDDAPYGGGAGMVMQPGPVCDAYEDLCRRLNRRPRVIYMTPQGQRFNQKIAEEL
                     SKEEELVFLCGHYEGIDERALEAIGAEYLSLGDFVLTGGELPAMVMIDCISRLVPGVL
                     GNGDSAEIESFYDNLLEYPQYTRPEVFEGRPVPEVLLTGHHKNIEAWRREQSIRRTLE
                     RRPDLLAEASLTLKEQKFLDSLKSELGQESILKELETYIEKLVDPECGESAGKQVRRQ
                     MALVKKLLNEKQCTAEELKGYFKVMALKESM"
     gene            12300..13583
                     /locus_tag="ACZPCG_03145"
     CDS             12300..13583
                     /locus_tag="ACZPCG_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HIR1284101.1"
                     /translation="MDLSGDTVVIRLVLVVVCLVLSAFFSSSETALTTVNLIRIRNLA
                     DNGDKAAAWVLKARRDQSKMLGAILIGNNVVNLSASSMLTVLVTDVFGSQAVGAATGV
                     LTLLVLLFGEITPKTIATLEAEKNALRFARVICLLMTILTPVIFVVNLLSGGVLRLLG
                     VDPNKPTDSITEDELRTIVEVGHEKGVIESEEKEMINNVFDLGDSVARDIMVPRIDMS
                     FVDVEASYEELMEIFRRDHYTRLPVYEDNTDNVIGIINMKDLILLEDRAAFSVRDYLR
                     QPLFTFESKKLSELMIEMRKTSNNIVIVLDEYGATAGLITLEDILEEIVGDIRDEFDA
                     DEEDELKEISKGEYLADGSMNLDDINDRLGLELVSEDFDSLGGFMIDRLEHLPAEGEE
                     VDTEEVRLVVEKVNKNRIDKVHIYVKKTVANEADL"
     gene            13698..14045
                     /gene="rplS"
                     /locus_tag="ACZPCG_03150"
     CDS             13698..14045
                     /gene="rplS"
                     /locus_tag="ACZPCG_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HIR1284102.1"
                     /translation="MNEIIKNIEAAQLKESVPSFHVGDTVKVYNKIKEGNRERIQIFE
                     GVVIKRQNGGARETFTVRKNSNGIGVEKTWPLHSPSVDNVEVVRKGKVRRAKLNYLRD
                     RVGKAAKVKELVK"
     gene            14125..14679
                     /gene="lepB"
                     /locus_tag="ACZPCG_03155"
     CDS             14125..14679
                     /gene="lepB"
                     /locus_tag="ACZPCG_03155"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR1284103.1"
                     /translation="MDFYINEDTAWLRRAVSWVVNIVVVLACAWFLVYGYGGQVEISG
                     NSMQPVLDGGDVVLTDRITYVVRRPRRFDIVVFKREDGRKNVKRVIGLPGETVQIKNG
                     LVYIDGSPLKAPEGLDLVSLAGRAESPVLLSEDEYFLLGDNRDSSEDSRFSNVGNVKE
                     SQILGRVWLRFLPLLKLDFIPGGQ"
     gene            14723..15640
                     /gene="ylqF"
                     /locus_tag="ACZPCG_03160"
     CDS             14723..15640
                     /gene="ylqF"
                     /locus_tag="ACZPCG_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HIR1284104.1"
                     /translation="MNIQWYPGHMTKARRAMKEDMKLIDLVIELVDARVPFSSRNPDI
                     DELAAGKARMVLLNKADLADEAKNAAWAALFESRGIHVAKIDARNKGSLKQVQAVIQE
                     ACKEKIERDRRRGILNRPIRTMVVGIPNVGKSTFINSFAGKACAKTGNKPGVTKGNQW
                     IRLNKTLELLDTPGILWPRFEDQTVGLHLALIGSINDQILNREELAWELIGLLEQIYP
                     GVLTERYGDAAEGETEDNSAWKELSKEEQNLKASRMLERIAKNRACLMKGGELDLARA
                     SALLLDEFRSGKLGKLSLEQAQDYTDRVK"
     gene            15641..16297
                     /locus_tag="ACZPCG_03165"
     CDS             15641..16297
                     /locus_tag="ACZPCG_03165"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HIR1284105.1"
                     /translation="MARELKGERLEKELARLEAMKAYEKGYEDYTLICGIDEAGRGPL
                     AGPVTAGAVILPKDCQILYLNDSKKLSEKRREELFLEIKEKAVSWCVGTASPARIDEI
                     NILQATYEAMREAIRGLSAVPEILLNDAVQIPKVEIPQVSIIKGDAKSVSIAAASILA
                     KVTRDHMMAEYDEIYPGYGFAKHKGYGTAAHIQALKELGPCPIHRRSFIKNFVAEEAE
                     "
     gene            16294..16635
                     /locus_tag="ACZPCG_03170"
     CDS             16294..16635
                     /locus_tag="ACZPCG_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HIR1284106.1"
                     /translation="MNKRETGSRYEDAAAAFLQSKGLRLLEKNFRCRKGEIDLILMDG
                     ACLVFTEVKYRSGPGCGSPLEAIDGRKQKKIRHTAAYYMYIRRISQDTPCRFDAVGIM
                     GESIQWIQDAF"
     gene            16671..17522
                     /gene="pgeF"
                     /locus_tag="ACZPCG_03175"
     CDS             16671..17522
                     /gene="pgeF"
                     /locus_tag="ACZPCG_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HIR1284107.1"
                     /translation="MIQWKYKNSAKVLTTREKGGVPYLSFDVLEKTGLVINGFSTRLG
                     GVSKGDCASMNFSYTRGDDPKDVLENFTRMATALGVDRDRMTVSWQTHTVNVRRMTKA
                     DEGKGVIIPRDYRDVDGMITDVPGQTLVTFYADCVPLYFVDRTHRAIGLSHSGWRGTV
                     KAMGQETIKAMKEAFGTRPEDLVVCIGPSICKDCFEVGEEVVEEFKEAFAPRWHSSLF
                     TEGKAPGKYQLDLWTANEILFLEAGLKKEQIHTANICTMCNHEVLFSHRKAGAKRGTL
                     AAFLCLK"
     gene            complement(17591..18775)
                     /locus_tag="ACZPCG_03180"
     CDS             complement(17591..18775)
                     /locus_tag="ACZPCG_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HIR1284108.1"
                     /translation="MANNFVFNDSLPVAPLKLAALESCRDFASKVNDHIVQFRRNDIE
                     ELTRRKADLRYRGYDVDSYLLDCHCPRFGTGEAKGVINESVRGNDVFVMVDVMNYSIP
                     YTVCGYTNHMSPDDHYQDLKRIIGACSSTAHRINVIMPFLYESRQHKRSKRESLDCAM
                     ALEELVAMGVENIITFDAHDPRVQNAIPLYGFDNFLPTYQFVKTLFSHDTTTKIDKEH
                     LMVISPDEGAMNRAVYLANNLGVDMGMFYKRRDYSKVVNGRNPIVAHEFLGSSVEGKT
                     VLIIDDMISSGESMLDTCKELKERKAAKAIVCCTFGLFTNGLEKFDEYFEKGYLDYVI
                     TTNLNYRPKELFDRKWYLEADMSKYTAAIINSLNHDRSIGATLSPTEKIQKLVRKRQQ
                     II"
     gene            19010..20425
                     /locus_tag="ACZPCG_03185"
     CDS             19010..20425
                     /locus_tag="ACZPCG_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002603974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HIR1284109.1"
                     /translation="MEKKLKGHLISKVDPGSIGEELELEPGDCVLTINGNPIEDIFDY
                     EYYVNSPSMLMTVRKADGEEWELDIENDYEDLGLTFENGLMSEYRSCSNKCIFCFIDQ
                     MPPGMRETLYFKDDDTRLSFLQGNYVTLTNLKEKDIERIIRFKLAPINISVQTTNPEL
                     RCKMLHNRFAGQSLKLIDRLYEAGTPMNGQIVLCKGVNDGEELKRSIEDLTKYIPCMQ
                     SVSVVPVGLSKYRDGLYPLEPFTKEDACQVIDLIEMWQKKLYKEHGIHFIHASDEWYI
                     LAERPLPEEERYDGYIQLENGVGMLRLLASEVEEALKGYSDDGQNRTVSIATGRLPYP
                     YIKKYAGWINEKFPGRTIHIYPIRNDFFGESITVSGLLTGQDVIAQLKGKELGEYLLL
                     PVNMFRSGETVFLDDLTAEDAEKALQVKVRIVKSSGRDFVKAVLYPELEAEQTEDEAG
                     LGADYGYEGYELDELSDESYK"
     gene            20441..21769
                     /gene="der"
                     /locus_tag="ACZPCG_03190"
     CDS             20441..21769
                     /gene="der"
                     /locus_tag="ACZPCG_03190"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HIR1284110.1"
                     /translation="MSKPVVAIVGRPNVGKSTLFNVIAGDTISIVKDTPGVTRDRIYA
                     DCSWLDMNFTLIDTGGIEPDTSDIILSQMREQAEIAISTADVIVFIVDVRQGLVDADS
                     KVADMLRKSRKPVVLAVNKVDSMAKFGNDVYEFYNLGIGDPVPVSAASRLGIGDLLDE
                     VVKHFDGTASEEEEDDRPRIAVVGKPNVGKSSIINKLLGENRVIVSDIAGTTRDAVDT
                     EIVHNGTPYVFIDTAGLRRKNKIKEELERYSIIRTVTAVERADIVVVVIDAKEGVTEQ
                     DAKIAGIAHDRGKGIIVAVNKWDAIEKNDKTIYQYTNKIRETLAFMPYAEFIFISAAT
                     GQRLPKLFDMIDMVRQNQNLRVATGVLNEIMTEAVAMQQPPSDKGKRLKIYYMTQVSV
                     KPPTFVVFVNDKELMHFSYTRYLENQIRNAFGFKGTSLKFIIRERKEKDQ"
     gene            21788..22417
                     /gene="plsY"
                     /locus_tag="ACZPCG_03195"
     CDS             21788..22417
                     /gene="plsY"
                     /locus_tag="ACZPCG_03195"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006780629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HIR1284111.1"
                     /translation="MERIICLAAGYLCGLFQTGYIYGKLHGIDIRSYGSGNSGSTNAL
                     RVMGKKAGLTVFVGDFLKTVIPCFITRLLFKDQPELMYVYMLYTGLGVILGHNFPFYL
                     GFKGGKGIAATAGIIFSIDWRLTVICLSCFILLVAVTRYVSLGSLVVSVILLVWDAVQ
                     NLRGAYGLIPGRLEFTAVTAVITVMAFWRHRANIGRLLKGTENKVGAKK"
     gene            22453..23463
                     /locus_tag="ACZPCG_03200"
     CDS             22453..23463
                     /locus_tag="ACZPCG_03200"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HIR1284112.1"
                     /translation="MSKIGVIGSGTWGTALAVLLTGNGHEVELWSAVPAEVEALTATR
                     RHPNLGDTPIPEKIRVTGDLEQAMKDKELLVLSVPSVYVRETAHRMAPFLKEGQVITN
                     VAKGIEDTTLKNLSEIIEEELPAARVTVLSGPSHAEEVSRGLPTTCVAGAHRRQDAEL
                     VQNLFMSPVFRVYTSPDMLGIELGGALKNVIALAAGIADGLGCGDNTKAALITRGIAE
                     ITRLGTAMGGSPETFSGLTGIGDLIVTCASMHSRNRRAGILIGKGYTMDEAMKEVKMV
                     VEGVYSARAARALSKKYQVSMPIVEQVNEVLFDGKPAKDALYDLMLRDRRAENALLEW
                     KE"
     gene            23629..24837
                     /locus_tag="ACZPCG_03205"
     CDS             23629..24837
                     /locus_tag="ACZPCG_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009534253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1284113.1"
                     /translation="MKKRLLCAGLAAAMAVSLTACGGSAAGTKAEGSSASAEANSEGG
                     SEAAKVFKIGGIGPVTGGAAVYGMAVQHGTELAVNEINAAGGINGYQIEFQFQDDEHD
                     AEKSVNAYNTLKDWGMQVLVGTTTSAPCLAVADKTAADNMFQITPSGSVVECAANPNV
                     FRICYSDPDQGAASATYIGEHKLASKIAVIYDSSDVYSSGVYEKFAQEAPAQGIEIVA
                     AEAFTADSNKDFSTQLQKAKDAGAEMVFLPIYYTEASLILQQANTMGFAPQFFGVDGM
                     DGILQVEGFDTSLAEGLMLLTPFAADAKDDLTVKFVKAYEDAYKETPIQFAADAYDAV
                     YAVKAAIEDAKATPDMSVSDLCEAMKASMLNIKLDGLTGTGMTWTEDGEPHKAPKAVK
                     IVDGAYAAME"
     gene            24921..>25033
                     /locus_tag="ACZPCG_03210"
     CDS             24921..>25033
                     /locus_tag="ACZPCG_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008725457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR1284114.1"
                     /translation="MSFVNYLINGVSLGSVYAIIALGYTMVYGIAKMLNFA"
BASE COUNT         6850 a         5242 c         7001 g         5940 t
ORIGIN      
        1 taaaccggct ggatgtgccg gtgcgccatg cgtttttgtc tctggcagtt catattatat
       61 tggtagaggt gttccttatg atattccata tggggatttt cagcgtggta tttgccaata
      121 tcctttttgc atttatgatg tgtctgttaa atggacgcag cattgccaga tatgcgggat
      181 atcgccagga atacaaaaag accgtgatcc ttccttttgt ctgttctctg ctcatgggag
      241 cagccgcctg gagcatatat aaaggacttg tttttttatg cccggccctt ttaagcggag
      301 gccgtttggg aatggctgtg ctggtatttc ccccggtggc tgtttctatg gctgtatatg
      361 gggtgcttct cgtgaagctt aaggcaatag atgaaaagga acttaaggag atgcctatgg
      421 gaacccgtct ggtacgcctg tgcaaacggg tacgcctgat gtagaaagaa agagattttc
      481 gagctggaca ggtgtgttta aaaatcagca gaacgcagat actagcttta caggaggtca
      541 ggatcatgaa acgaatgtat tgctatgcgc tgctgttttt gggaaccgct tttttgtgtc
      601 tggttcttat ctacgggctt accagatacc gcatccgtca ggaagagccg attcccaata
      661 ccatactgga aacagagacg gtgcgggata tggagggacg ggaggctgta aatcagagcg
      721 gggttcttcc ggcagcaaaa atgacacagg aagagccgaa ggaggaatac tttctggtat
      781 ctgaggcagg atttttactg gtattctgca gcgataaaag cactatctgt ctgtataccc
      841 atattccggt cactgatttt ccggaaaggg agagagcgcg gctgatggag ggaatctggt
      901 ttccctctat gatggatgta taccattatc tggaatcgta tacaagttaa aagaaaatgt
      961 ttcagcgggc ggaatctgcc gcccctgttg cagtcaggag taaaacaaaa cagatgaaaa
     1021 acagaacagt tggaattata ggaggagggg cttccgggct gatggcagca atttcggctg
     1081 caagagcggg agcgcaggtg actctaatcg aacatatgga ccgggttgga aagaaaatcc
     1141 tttctaccgg gaacggaaga tgcaatctga cgaactacag gatggagccg gaatgctacc
     1201 gctgcagcca gaaggatttt cccatgaagg taattcaggg ctttggcgtg gaggaaacgc
     1261 tggagttttt taagtatctg ggaatcgtgc caaaagacag gaacggatat gtgtatccta
     1321 attccgatca ggcagcgtct gttcttgatg tccttcggtg ggagacggag cggcttggga
     1381 tcagggtgct tctttcctgt caggtatctg tcataaagcc ccagagggac gggggatttc
     1441 tgatccggag cagccaggga acgcttcata cagataccct gattctggcg gcaggaagca
     1501 aggccgctcc ttctaccggt tcggacggaa gcggttatga acttgcgaaa gctttaggcc
     1561 ataaggtaat taagcctctt ccggcgctgg tgcagcttcg gtgccaggga acgctttata
     1621 aacagctggc aggaatccgc acagaggcca ggatccgtct tctggcggac ggtatatgca
     1681 tggcagagga taaaggggag ctgcagctga ccgattacgg cctgtccggg attccggtat
     1741 ttcagatcag ccgttacgct tcaagggcat tggacaaggg ttccagagta agagccagta
     1801 ttgactttat gccatcctgg aaggaagatg aggcgttccg tcttcttaaa aaacgggcag
     1861 tcatgctgaa ggaaaagcca gtgggagagc tttttacggg aatgttaaac agaaaacttg
     1921 cgcaagtgct gatcaaactg tccggtatcg atccatctgc tctctgtgaa cgtcttggaa
     1981 accgccagct ggaacggatc ctgtatcagc ttaaaagcta tgaggccatt gttatgtccg
     2041 taaatccatt ttccaatgct caggtgtgct gcggaggagt tgatacaaga gaggtggatc
     2101 cggataccat ggagtccaga ataaggacgg ggctttatct ggccggagag cttctggatg
     2161 tagacgggat ctgcggcgga tataatctgc agtttgcatg gtccagcgga tggatcgccg
     2221 gacagtcggc agccggaata gagaagccgc acagaaaata aagaaagagg aaaataaata
     2281 catatgatac ggattaatca gctgaagctg cctctggagt acgaaaaaga aaaacttttg
     2341 cagaaagcgg caaggatact ggggatttct cctgctgata ttttgaaaat cgatattata
     2401 aaagagtcca ttgacgccag aaagaaaccg gaaatttttt acagctatat tgtggatgtg
     2461 actttaaaat ccggaggctc aaagagggaa gaatccattg tcagaaaaaa aaaggaccgt
     2521 aatttatcaa tcagccatga gaagccatac tgcctgccgg agtgtggaac tgcccctctg
     2581 gcataccctc cggttattat cgggactggt ccggccggac tgttctgcgg tctgatgctg
     2641 gcaagaaaag gatactgccc cattctttta gagcggggtg aggacgtaga cgccagaacc
     2701 agacgggtgg aagctttctg gaaggatggg gtattaaatc cagcatccaa tgtacagttt
     2761 ggtgaaggcg gagcaggcac tttttcagat ggaaagctga atacgctggt aaaggatacc
     2821 tttggacgaa acagagaggt tcttaaaatt ctttctcagt tcggtgcaga cccttccatc
     2881 tgctatcaga ataagcctca tattggaacg gatgttttaa gccggatcgt aaaatccatc
     2941 cgccaggaga tcgaatctct gggcggaatt gtgcgttttt taagtcaggt tacggatttt
     3001 aggataaagg atggaaagct gtatgctttg gagattaatg gaaaagaact cctgtataca
     3061 gatgccgctg tactggcagt aggacacagc gccagggata cgtttgagac actttaccgc
     3121 cacagcgttc ctatggaggc gaaagccttt gccgtgggac tgcgtatcca gcatcctcag
     3181 gccatgatca atatgagcca gtacggcatg gaggagtgca gcgcgttagg acctgccagt
     3241 tataagctga cccggcagac tacagcaggc agaggggtct attccttctg catgtgccca
     3301 ggcggatatg tggtaaatgc gtcctctgag cctggaagac tggccgtaaa cggtatgagc
     3361 taccatgaca gggcaggaga gaatgccaac agcgcgatca ttgtgtctgt gaccccagag
     3421 gatttttctc acgacaatga tgagaaaaat cctctaagcg gaatttactt ccagagagaa
     3481 ctggaggaaa aggcgtatca ggcagccgga ggaaacattc cggtacagct ttatggagat
     3541 tttatctcgg actgcacttc agatcacttt ggcaaggttc ggccggcttt cagggggcag
     3601 tatgggtttg cgaacctgcg ccggatcttg ccggaggcat tgtctgcttc ttttatagag
     3661 gctatggaag gtttcggcca tatgataaag ggctttgact gtccggatgc gattctggca
     3721 ggaattgaaa gccgcacttc atcgcctgtt cggattccca gagatgaaac gcttgaaagt
     3781 gcggttcgcg gcctttttcc atgtggggaa ggggcaggct atgcaggggg gatcacctcc
     3841 gcggccatgg atggcatcaa ggtagcggaa gagctgatcc gcagataccg gcctcttaag
     3901 gagaaaacgg gagaacttga gggataaaaa aattgacggg ctatcaaatt tggtgtaaga
     3961 taaaaaacat ggcgtttcag cgcctgaaag cagaagaaaa aggagcagta tcatggaagc
     4021 acaggaacga aagaacctga aggataaaaa aagaatcgtc attaagattg gttcctcctc
     4081 tctaacccat ccggaaacag gggagttaaa tcttatgaaa atagagaagc tgatccgcac
     4141 cttaagcgat ttaagaggac agggaaagga cgtggttttg gtatcttccg gcgccattgc
     4201 cgcaggaagg caggctctgg gccatcacaa gcgcccggac tccctggcgg aaaaacaggc
     4261 ctttgccgct gtggggcagg cccggctgat gatggtgtat cagaagctct gcgcggaata
     4321 caaccagaca gccgctcagg tgcttcttac aaaggatacc atggtaaatg acgcttcccg
     4381 ctacaatgcc cagaatacct ttgaggagct tttaaatctg ggaacgattc ccattgtaaa
     4441 tgaaaacgat accgtttcca cctctgagat tccttatgtg gacagctttg gagacaatga
     4501 ccgcctctcc gcgatcgtag ctgctttgat cggagcagat cttctgatcc ttttatcgga
     4561 tattgacggt ctttatacag atgatcccag aactaataag gaagcccgct ttatcagttt
     4621 tgtgccggaa atcacaaagg agcttctttc catgggaaag ggaacctcag gaagcgatgt
     4681 gggcacagga ggtatgtctg ccaagctggc agcagcccgg atcgccacag acagcggagc
     4741 ggatatggta attgccaatg gagaccatgt agaggcagta atggaaattg taggagggaa
     4801 agaaacagga accctgtttt tagcccatac taatctggat tttgacctga tgcattactt
     4861 aaataacgag tattaaggac aggaggagaa aatggacgca aaaaagatag atcggatcaa
     4921 cgaactttac cataaatcca agtctgtagg actgacggag aaagaaaaaa gtgagcaggc
     4981 ggctctgcgc agggagtatc ttgcagcaat cagaggaagc ctgagaaata atttaaataa
     5041 tatttctatc gtagaaaagg acggatctgt tacagatctt ggaaagaaat acggcggtat
     5101 caaagaggtg tagcagcccg gagaatgagc gaaaaggagc gcagagggaa atatgacatt
     5161 aaatgagatc ggcggtcagg ccagggcagt ttccagaata ctgaacagcc tgggctccag
     5221 agaaaaaaat attggattgg aggaagcagc cagagcgctg cttgagggcg aggatgagat
     5281 ccttgctgca aatgaggcag attatgagat ggcacggcaa aagggcatga gccagggact
     5341 tttagaccgt ctgcgtttga ctccggccag agttcaatcc atggcagacg gacttttaaa
     5401 ggtcgcttct ctggaggatc ctgtgggaga agtcatctcc atgaagcttc gtcccaatgg
     5461 tttaaaaatt ggccagaaac gggtgccttt aggcgtgatc ggcatgatct acgaggcgcg
     5521 tcccaatgtg acagccgacg ccttcggcct gtgctttaag tccggaaatg ctgtgatctt
     5581 aaagggtgga agcgatgccg gagagtccaa taaggctatt gtaagatgga tccgcaccgg
     5641 tcttgtccgt gcgggactgc cggaggatgc tgtgcagctc attgaagatg ccagccggga
     5701 aactacgaga gaactgatgc gcttgaatca gtatgtggat gtgctgatcc ccagaggcgg
     5761 agccggactg atccgtacgg ttgtggaaaa cagtaccgtg cctgtgatcc agacaggaac
     5821 aggaaactgc catatttttg tagacgagtc tgcggatttc catatggctc tggatattat
     5881 cttcaatgca aagacccaga ggatcggggt ctgcaatgcc tgtgaatcat tggtggtcca
     5941 cagaaaaatt gcagcggact tccttccatt attaaggaaa aaactggaag aaaagcaggt
     6001 ggagatccgg gcggatgagg cagccagagc cattgaaccg tcctttaagc cggcagagcc
     6061 ggaggactgg ggaacggaat atctggatta catcctgtcc ttaaagctgg tggattccct
     6121 ggatgaagcc attcgtcata tcaatacata caataccggt cattctgagg ccattatcac
     6181 ctctgattat tttaacgcac agaagttttt ggatgaggtg gatgcggcag ctgtgtacgt
     6241 aaatgcctcc acccgtttta ccgacggaga ggaattcggt tttggagcgg agatcgggat
     6301 cagcacccag aagcttcacg ccagaggccc tatgggctta aaggagctga ctacaaccaa
     6361 gtatatcatc tacggagacg gccagatccg cagctgagaa gagaggaact tatttatatg
     6421 ttagaagaag aaaagaaggg cagaagccgc aagaaagaag aggaggagcc ctttgatctg
     6481 aaaaaggaga tcatcagctg gatccagatt attgcggcag ctgtgatcat tgcccttgtg
     6541 ctgaataatt tcattattgc caacagccgt gttcccagcg gttctatgga aaataccatt
     6601 atgaccaaaa gccgtgtgat cggctccagg ctttcctact taaccgatga cccggagcgg
     6661 ggagatatcg tgattttcca ttacccggat gatgaatccg tttactatgt aaagcgcgtg
     6721 atcggccttc cgggagaaac ggtggagatc gtagacggaa aggtttatat taatggctct
     6781 gacacaccct tagaggaacc ttatcttcct gagcctatgg agggctccta cggtccttac
     6841 acagtgcctg agggaagcta tttcatgctg ggcgacaacc gcaacaattc cagagatgcc
     6901 agatactggg aaaacaagtt cgtaaaaaag gaaaagatca tagcaaaggt attattctgt
     6961 tattatcctc tttcccaatt cggacctgtg gaataaatag agattaccac cggaaccggc
     7021 tgggcttatc tgcccagccg ccttacgttg ccggaaaaga aacattatac catcagaaca
     7081 ggagtaaaca tacatggaat tatctgcaaa catctcttat gaggcgctgc acggcgctcc
     7141 tccggcctgt gaaccggaac gccagcagta ttttatggaa aaagcaaaga agcagctgga
     7201 ggtttttaag gatcgtttgg gccgtcctct gacctgctgt atcaatacct ttggctgcca
     7261 gatgaacgcc agagattcgg agaaactttt agggatcctg gaagccatcg gctatgtggc
     7321 atcggacacg gaagaggcgg attttgttct ctataatacc tgtacagttc gtgaaaatgc
     7381 caatttgaga gtttacggcc gtttaggaca gcttggagca agaaagaaaa agcatcccca
     7441 tatgatgatc gcgctctgcg gctgtatgat gcaggaaaag gaagtggtag aaaagatcag
     7501 aaagagctac cgctatgtgg atctgatctt tgggacccac aatattttta agctggcaga
     7561 gctgatcagc atctgcctgg aaaaacgcca ggaagagctt ctgaacgagg aggaaggcgt
     7621 ccatacaaaa cacaccatgg tggtggacgt atggaaggac acggatatga tcgtggagga
     7681 tcttccggtg gagcgcaaat attcctttaa atccggcgtc aatattatgt ttggctgcaa
     7741 caatttctgc agctactgta ttgtccccta tgtccggggc agggagcgca gcagaaagcc
     7801 ggaggagatt ataaaggaaa tcaagcggct ggtggcagac ggcgtagtgg aggtaatgct
     7861 tttggggcag aatgtaaact cctatggcaa aaatctggag acgcccatga cctttgcaga
     7921 gctgcttcgc gagattgaga agattccggg actggagcgg atacgcttta tgacctccca
     7981 tccaaaggat ctttcggatg aactgattca ggtaatggca gagtcccaaa agatctgccg
     8041 ccatctccat ctgccccttc agtccgggag ctcccgcatt ttaaaggaga tgaaccgaag
     8101 atataccaag gagcagtttc tggctctggc ggaaaagatc aaggccgctg tgccgggtat
     8161 ttccctgacg acggatatta tcgtgggctt tccaggagag acagaagagg attttgagga
     8221 aaccctggat gtggtaagaa acgtacgctt tgacagtgca tttaccttta tctattccaa
     8281 gcgcacggga actcctgcgg cggccatgga aaaccaggtg cctgaggacg tggtaaagga
     8341 acgctttgac cgtcttttaa aagaagtaca ggatatttcc gcagaggtct gcggacggga
     8401 tgtgcatacg gtgcagcagg tgctggtgga ggaggtaaac gaccattctc ctgagctgat
     8461 gacgggccgt atgtcaaata ataccgtagt acattttccg ggagatgcgt ctatgatcgg
     8521 ttccctggta gatgtttatc tggaagagag caggggcttt tactacatgg gacggatcca
     8581 tgaggattca ggggaggcgt gatcgtggag attgatattt cacagctgtc tcccatgatg
     8641 cagcattaca tggagacaaa aaaagaatat ccggactgta tcctgtttta ccgtctgggc
     8701 gatttttacg aaatgttttt cgatgatgcc ctggcagctt ccaaagcatt ggagattacg
     8761 ctgaccggaa aggaatgtgg ccttaaggaa cgtgccccca tgtgcggagt tccctatcat
     8821 gcagtggatt cctatctgta ccgtctggtt caaaagggtt ttaaggtagc cattgcagag
     8881 cagatggaag acccaaaaca ggcaaaaggg cttgtaaaga gagaggttat ccgggtggta
     8941 acgccgggca ccattaccag cgctcaggct ctggatgaga cgaaaaataa ttacctgatg
     9001 ggaattgtct atgtggacgg ccgctacggt atttccaccg cagatatcag tacgggagaa
     9061 tttttggtta cagaagtgga ttctgaccgg gagctgtttg atgagatcaa taaattttct
     9121 ccctctgaga tcatctgcaa caatgcattt tatatgtctg gggtagacct ggatgaactg
     9181 aaaaaccggt atcaggtcat catatccgct ctggacagcc atttctttgg ggatgaatcc
     9241 tgtaaaaaag tattaaagga gcactttaag gtaaattcat taagtgggct gggattagag
     9301 gactatgcta cggggatcat tgcagcagga gcggtgatgg aatatatgta tgagactcag
     9361 aagagcacct tagagcatat tacttccctc accccctacc gcaccggcca gtatatggtg
     9421 atcgacacct ctaccagaag gaatctggag cttgtggaaa ccatgcggga aaaacagaag
     9481 aggggcactc ttttgtgggt tcttgaccgg acaaagacag ccatgggcgc caggctgctg
     9541 cgaagctata tcgagcagcc gctgatccac aaagatgaga tcatccggcg gcaggatgcg
     9601 gtggaagagc tgaatatgaa ttatatttcc agggaggagc tgggagagta tttaaatccc
     9661 atttatgacc tggagcgcct gatcggacgg atcagctata agacggccag tcccagagac
     9721 ctcattgcct ttaagacctc tctggaaatg ctgcctcata tcaaacgcct tcttggagag
     9781 tttcagaccg ggcttttaaa ggagcttgag agcgagctgg atcctttgga agatatttgg
     9841 gatctgatcc gccgttccat tgtagatgat ccgcccatta ctgtgagaga cggcggtatc
     9901 attaaggaag gcttccatga ggaagccgac cggctgcgca atgctaaaac cgagggaaag
     9961 acctggttag ctgagttaga agcacgtgaa aaggaaaaga cgggcattaa aaccctgaag
    10021 gtcaagtaca ataaggtgtt cggctactat tttgaagtga caaattcttt taaagacatg
    10081 gtgccggact attttgtgcg caagcagacc cttacgaatg cagagcgctt taccacagat
    10141 gagctaaagc agcttgagga tgtgatcatg ggggcggagg aaaagctgac agccctggaa
    10201 tacgatctgt tctgtgaggt aagggattcc atttcttccc aggtagtacg gatccagcag
    10261 acggccagag ccattgcggg aatcgacgtg ttctgttccc tgtcctctgt ggcgacccgc
    10321 cgcaattacg tcaagccctc tatcaatgaa aagggcgtga tccacatcaa gaacggccgc
    10381 catccggtag tggagcagat gatgcgggat gatctgtttg tggcaaatga taccttttta
    10441 gacaatggaa agaaccgtct ttccgtaatt accggtccca acatggcggg aaaatctacc
    10501 tatatgcgcc aggtggctct gatcgtgcta atggcacagt taggaagctt tgtaccggct
    10561 caggaggcgg atatcggtat ctgcgaccgt atttttaccc gtgtgggcgc ttcggatgat
    10621 ctagcaagcg gacagagtac ctttatggtg gagatgaccg aggtggcaaa tatccttaga
    10681 aacgccacaa ggaacagcct tctggtgctg gatgagatcg gccgcggcac cagcaccttt
    10741 gacggccttt cgatcgcatg ggccgtgatc gagcacatca gcagcacaaa gcttttggga
    10801 gcaaagacgc tttttgctac ccattaccat gagctgacgg aattagaggg taccattgca
    10861 ggcgtgaaaa attactgtat tgcagtaaag gagcagggag acgatattgt atttttaagg
    10921 aagattgtcc ggggcggcgc agataagagc tacggcatcc aggtggcaaa gctggccggg
    10981 gttccggatt ctgtcatcag ccgcgccagg gagatcgccg gagagctcag tgaaaatgat
    11041 attacggccc gtgcaaagga aatcgctcag gcgaatgcca atgtgaccca gcacagggcg
    11101 gttccaaagc cggatgaggt ggatctgcag cagcttacct tctttgatac ggtaaaggat
    11161 gatgatatta ttaaggagct ggacggcctg gagctttcca ctatgacgcc gcttgatgcc
    11221 ttaaataccc tgtaccggct tcagacaaag ctgaaaaacc gatggaagga gtaaatatac
    11281 atgaattttc atattcttac cctgtttcct gacatggtta tggatggtct tggcaccagc
    11341 atcacaggac gggccatgga aagcggggcc attcaggcag aggcggtgga cattcggact
    11401 tattcaaggg acaagcaccg ccatgtggat gacgcgcctt atggcggcgg agcaggcatg
    11461 gtaatgcagc ccggtccggt gtgcgatgcc tatgaggatc tgtgccgccg cttaaaccgc
    11521 cgtcccagag taatctatat gacccctcag ggccagcggt ttaaccagaa gattgcggag
    11581 gagctgtcta aggaggaaga gcttgtattc ctctgcggcc actatgaggg cattgatgag
    11641 cgggctctgg aggccatcgg tgcagagtac ctttctcttg gggattttgt ccttaccgga
    11701 ggggagcttc ctgcaatggt gatgatcgac tgcatttccc gactggtgcc aggggtgctg
    11761 gggaacggag attctgcaga aatagagtcc ttttatgaca atcttttaga gtatccccag
    11821 tatacccgcc cggaggtctt tgaaggccgt ccggtgccgg aggttctcct cacaggccat
    11881 cataaaaata ttgaagcctg gagacgggag caatccatcc gccgaaccct tgagcgccgt
    11941 ccggatcttc tggcagaagc cagtctgact ttaaaggagc agaagttttt ggacagcctt
    12001 aagtctgaac tgggacagga gagcatttta aaggaactgg aaacatacat tgaaaagctt
    12061 gtggatccgg aatgcggaga gagcgccgga aaacaggtgc gccgccagat ggctctggta
    12121 aagaagcttt tgaatgaaaa acagtgcacg gcagaggaat taaagggcta ttttaaggtc
    12181 atggctttaa aggaaagtat gtaaaaattt taacgcttat tgacaaggga agggcttcta
    12241 gtgtaaaatt ccttgaaacc tgtgtaaaaa taaacaaaag aaataggagt tgatgaattt
    12301 tggatctatc aggtgatacg gtcgtcatac gtttggtgtt agtagtggtc tgtctggtgc
    12361 tttcggcgtt tttctcttct tcggaaaccg cccttacaac ggtgaatctg atccgtatac
    12421 gaaatcttgc agacaacgga gataaggcgg cagcctgggt tttaaaggcc agaagggatc
    12481 agagcaaaat gctgggagcc attttgatcg gaaacaatgt ggttaatctt tctgcgtcct
    12541 ccatgcttac cgtgctggta acggatgtat tcggaagcca ggccgtagga gcggctaccg
    12601 gagtgttgac tcttttagtg ctgctatttg gagagattac gccaaaaacc atagctactc
    12661 tggaggcaga aaaaaacgcc cttcgctttg cgagagtaat ctgccttctc atgaccatac
    12721 tgacaccagt tatttttgtt gtaaacctgc tctccggcgg ggttctgcgt cttttgggcg
    12781 tggatcccaa caagccgaca gacagtatta cggaagatga gctgagaacg atcgtagagg
    12841 taggtcacga aaagggtgtg atcgaatcag aagaaaaaga gatgatcaac aatgtgttcg
    12901 atctgggaga ttctgttgcc agagatatca tggttcccag gatcgacatg agctttgtag
    12961 atgtggaggc gtcctatgag gaactgatgg aaattttccg cagggatcat tatacccgcc
    13021 ttcctgtcta tgaggacaac accgacaatg tcatcggcat tatcaacatg aaggatctga
    13081 tccttttgga ggacagagcc gctttttccg tccgcgacta cctgcgccag cccctgttta
    13141 cctttgagtc aaagaaactc tccgaactca tgatcgagat gcgaaagaca tcaaataata
    13201 tcgtgatcgt tctggatgag tatggtgcaa ccgcaggcct gatcacgctg gaggatatcc
    13261 tggaggagat cgtaggcgat atcagggatg aatttgatgc agatgaggaa gacgagctta
    13321 aggagatttc caaaggagaa tatctggcag acggttccat gaacctggac gatatcaatg
    13381 accggcttgg cctggagctt gtatcagaag attttgattc actgggcggt tttatgatcg
    13441 accgtctgga gcatcttccg gcagaaggag aagaggtgga cacagaagaa gtccgtctgg
    13501 tagtggaaaa agtcaataaa aaccggattg acaaggttca tatttatgta aaaaaaacgg
    13561 ttgcaaatga agcggatctg tgataaactg attccgttgt gacaaagaat agatggtcct
    13621 ctgctactga attgcagtga acacgtttta ccgcatagag tattaagaac atccaataaa
    13681 tgaaggaggt tcacacaatg aacgaaatta tcaagaatat cgaagcagca cagttaaagg
    13741 aaagcgtacc tagcttccac gtaggtgata ccgtaaaggt atacaacaag atcaaagagg
    13801 gtaaccgcga gagaatccag atctttgagg gcgttgtgat caagagacag aacggcggag
    13861 caagagagac cttcaccgtt agaaagaact ccaacggcat cggcgtagag aagacatggc
    13921 cattacattc cccaagcgta gacaacgttg aggttgtccg caagggtaaa gtaagaagag
    13981 caaaactgaa ctacttaaga gacagagtag gtaaggctgc taaggtaaaa gagttagtta
    14041 aataagtaag acagacaaaa cggggacaat gaaaattgtc cccattttag tagctgcgga
    14101 ttgaaaacag aatcgggggc agacttggat ttttatatca atgaagatac cgcctggctt
    14161 cgcagggcag tcagctgggt ggtgaatatt gtagtagttt tagcctgcgc ctggttttta
    14221 gtctatggtt atgggggcca ggtggaaatc tcaggcaatt ccatgcagcc agttttagac
    14281 gggggcgacg tggtgcttac ggaccggatc acttatgtgg tgagacgtcc caggcgtttt
    14341 gacatcgtag tgtttaaacg ggaagatgga aggaaaaatg taaaaagggt cattgggctt
    14401 cccggagaaa cggtacagat taaaaatggc cttgtttata ttgatggaag ccccttaaaa
    14461 gcccctgagg gactggatct tgtttcttta gccggacgtg cagaaagtcc ggtccttctg
    14521 tcggaggatg aatactttct gctgggagac aacagggaca gcagtgagga cagtcgtttt
    14581 tccaatgtgg gaaatgtgaa ggaaagtcag atcctcggcc gggtatggct gcgttttctt
    14641 cccctgttaa agcttgattt tatccccgga ggacagtgaa aaaccgggca gtaaaaatga
    14701 gaagaacgag gagttataag cgatgaatat acagtggtac ccgggccata tgacaaaggc
    14761 cagacgggca atgaaagagg atatgaagct cattgatctg gtgatcgagc tggtggacgc
    14821 aagagtgccc ttttccagtc gaaatccaga tattgacgag ctggcagccg ggaaggcgcg
    14881 gatggttctg ttaaataagg cagatctggc agacgaggcg aaaaatgcag cctgggccgc
    14941 tctgtttgaa tcacggggca tccatgtggc aaagattgac gccagaaata aaggaagctt
    15001 aaagcaggtt caggctgtga tccaggaggc ttgtaaggaa aagattgaaa gagaccgcag
    15061 aagagggatt ttaaaccgtc ccatccgtac gatggtagtg ggaatcccca atgttggaaa
    15121 gtccaccttt atcaattcat ttgccggaaa ggcctgtgcc aaaacgggaa ataagcccgg
    15181 tgtcacaaag ggaaaccagt ggatccgtct gaataaaacg ctggagcttt tagatactcc
    15241 gggtattttg tggcccagat ttgaggatca gaccgtaggt ctgcatctgg ctctgatcgg
    15301 ctccattaac gaccagattt taaaccggga agagctggcc tgggagttga tcgggctttt
    15361 ggagcagatt tatcccggcg tgctcacaga gcgctacggg gatgctgctg agggagaaac
    15421 ggaagacaac agtgcctgga aggaactgtc aaaagaggag cagaatctga aggcaagccg
    15481 gatgctggag cggattgcga aaaaccgtgc gtgcctgatg aagggaggag agctggatct
    15541 tgccagagcc tctgcccttc ttttagatga gttccgttcc ggaaagcttg gaaagctcag
    15601 tctggagcag gcacaggatt acacagacag ggtgaaataa atggcgcgtg aattaaaggg
    15661 agaacgtctg gaaaaggagc ttgccagact ggaggcgatg aaagcctatg aaaaaggata
    15721 tgaagattat acgttgatct gcgggattga tgaggccggc agggggcccc ttgcagggcc
    15781 ggttacggca ggggcagtga ttctgccaaa ggactgtcag atcctctatc tgaatgattc
    15841 taaaaagctg tcagaaaaga ggcgggaaga gctttttctt gagattaagg aaaaggctgt
    15901 atcctggtgc gtagggactg cttctccggc ccggattgat gaaatcaata ttctgcaggc
    15961 cacctatgag gccatgaggg aagcaatccg ggggctttct gcggtaccgg agattctgct
    16021 caatgacgcg gtccagattc caaaggtaga gatcccgcag gtatctatta taaagggaga
    16081 tgcaaagagc gtatccatcg ctgccgccag tattctggca aaggttacaa gggatcacat
    16141 gatggcagaa tatgatgaaa tctatcccgg ttacggcttt gcaaagcaca agggttacgg
    16201 cacggcggcc catattcagg ctttaaagga gcttgggccc tgtccgatcc acagaagaag
    16261 ctttattaaa aactttgttg cagaggaagc agaatgaata aaagagaaac aggcagccgt
    16321 tatgaagatg cagcggctgc ctttcttcaa agtaaagggc tgcggcttct ggaaaagaat
    16381 ttccgctgcc gcaaagggga gattgacctg atcctgatgg acggcgcctg ccttgtattt
    16441 acagaagtaa aatacagatc cggtccgggc tgcggctctc ccctggaggc cattgatgga
    16501 cgcaaacaga aaaaaatccg ccatacggcc gcttattata tgtatatccg ccgcatttct
    16561 caggatacac cctgccgctt tgacgcagta ggtattatgg gagaatcaat tcagtggata
    16621 caggatgctt tttaaaagaa ttgattaaaa gatacagggg agaatgctct atgattcagt
    16681 ggaagtataa aaacagtgcg aaggttctta cgacaagaga aaagggagga gtgccgtatc
    16741 tttcctttga tgttcttgaa aaaaccggcc ttgtgatcaa cggtttttcc accaggcttg
    16801 gaggcgtaag caaaggggac tgcgcatcta tgaacttttc ttataccaga ggggacgatc
    16861 cgaaggatgt attggaaaat tttaccagaa tggccacggc tcttggggta gacagagacc
    16921 ggatgaccgt atcctggcag acccatacag tcaatgtccg gcgtatgacc aaggcagatg
    16981 agggaaaagg ggtgatcatt cccagagatt acagggatgt agacgggatg atcacagatg
    17041 tacccggcca gacccttgtg accttttatg cggactgtgt gccgctgtat tttgtggatc
    17101 ggacccatag ggccatcggc ttaagccatt ccggctggcg ggggacggta aaggccatgg
    17161 ggcaggaaac tataaaggcc atgaaggagg ccttcggaac acggcctgag gatctggtgg
    17221 tctgcatcgg ccccagcatc tgtaaggatt gctttgaagt gggagaagag gtggtggagg
    17281 aatttaaaga agcctttgct cccagatggc attcttccct ttttacagaa ggaaaggctc
    17341 cgggcaaata tcagctggat ttgtggacgg caaatgaaat cctttttctg gaagctggac
    17401 taaaaaaaga gcagatccac acggccaata tctgtaccat gtgcaaccat gaggtgcttt
    17461 tttcccaccg gaaggctggg gctaaaagag gaactctggc agcattttta tgcctgaaat
    17521 gatacggcaa tgaaaaaccc cgtcttcgga cggcccttga tggcggcccg gacggggttt
    17581 catgatatca ttagatgatc tgctgtcttt ttcttacaag cttctggatc ttctctgtag
    17641 gagaaagggt tgctccgatg gaacggtcat ggtttaagga gttgataata gcagcggtgt
    17701 atttgctcat atctgcctcc agataccact tgcggtcaaa gagctccttt ggacggtaat
    17761 ttaagttggt tgtgatcaca taatccagat aacccttctc aaaatactcg tcgaacttct
    17821 caagtccatt ggtgaacagg ccgaaggtac agcatacgat cgccttggca gcctttctct
    17881 cctttaattc cttacaggta tccagcatac tttcaccgga ggagatcata tcgtcaatga
    17941 tcagaacggt cttgccttct acggaggaac ctaagaactc atgggctacg atggggttgc
    18001 gtccgtttac aaccttggaa tagtcgcggc gcttgtagaa catacccata tctacgccca
    18061 ggttgtttgc cagatatacc gcgcggttca tggcgccttc atccgggctg atcaccatca
    18121 gatgctcctt gtcgatcttt gtggtagtat cgtgggagaa cagtgttttt acaaactgat
    18181 acgtaggcag gaagttgtca aagccgtaaa gggggatggc attctgcact ctggggtcgt
    18241 gagcatcaaa ggtaatgatg ttttctacgc ccattgcaac cagctcctcc agtgccatag
    18301 cacagtctaa ggactcgcgc ttgctgcgct tgtgctggcg gctttcatag aggaaaggca
    18361 taattacatt gatccggtga gctgtagagg agcaggcacc gataatacgc tttaaatcct
    18421 gataatggtc atccggggac atgtggttgg tgtagccgca tacagtgtag gggatgctgt
    18481 agttcattac atcaaccatt acaaatacat cgttgccgcg tacagattca ttgatcacgc
    18541 cttttgcctc gccagtgcca aagcgcgggc agtggcagtc caacagatag gaatccacat
    18601 catatccgcg gtagcgcagg tctgccttgc ggcgggtcag ttcctcaata tcgttgcggc
    18661 ggaactgcac aatgtgatcg ttgaccttgg aggcgaagtc ccggcagctt tccagggcag
    18721 ccagctttaa aggtgcaacg gggagggaat cattaaatac gaaattgtta gccatgaaag
    18781 cttactcctt tgtccattgt agtgacggtt ttcctcagaa aacaaggcgg acaggccgtc
    18841 cctgtctgag ggcatacata tgatatatac catttcccgc cattctggtc aaggctttac
    18901 agagatttta ctgaaaaata cattgggaag ttgccattta caaacatagg taaaattgtt
    18961 atgatagaaa aagaacatca gaatacagac agattgaggt taaaataata tggaaaagaa
    19021 actgaaggga catcttattt caaaggtaga tccgggttcc attggcgaag aactggagct
    19081 ggagccggga gactgtgtcc ttacgatcaa cggaaatccc atagaggata tattcgatta
    19141 tgaatactat gtaaacagtc cgtccatgct tatgacggtg agaaaggctg acggagaaga
    19201 gtgggagctt gacattgaaa acgattatga ggatctggga cttacgtttg aaaacggact
    19261 tatgagcgag taccgatcct gcagcaacaa gtgcattttc tgctttattg accagatgcc
    19321 tccgggaatg cgggaaacac tgtattttaa ggatgatgat accagactgt cctttctcca
    19381 gggaaattat gtgaccctta cgaatctgaa ggaaaaagat atcgagcgga tcatccgctt
    19441 taaactggct ccgatcaata tttccgtcca gaccaccaat ccggagcttc gctgcaagat
    19501 gctgcacaac cgttttgcag gacaatccct gaaactgatc gaccgccttt atgaggcagg
    19561 tacgcccatg aacggacaga ttgttttgtg caagggtgtc aatgacgggg aggagttaaa
    19621 gcgttccatt gaggatctta caaagtatat tccctgtatg cagagcgtat ctgtggtgcc
    19681 ggtggggctt tccaagtaca gagacggtct gtacccctta gaaccgttta caaaggagga
    19741 cgcctgccag gtcatcgacc tgatcgagat gtggcagaaa aagctgtata aggagcatgg
    19801 gatccacttt atccacgcca gcgacgagtg gtatattctg gcagagcgcc cccttccgga
    19861 ggaggagcga tatgacggtt atatccagct ggaaaacgga gtgggaatgc tgaggcttct
    19921 ggcctcagaa gtagaggagg cgttaaaagg gtactctgat gacggtcaaa accgtaccgt
    19981 ttccattgca accggccgcc ttccctatcc ttatataaag aaatatgccg gatggatcaa
    20041 tgaaaaattc cccggccgca ccattcatat ttatcccata agaaatgatt tttttggaga
    20101 atccatcact gtatccggcc ttctcaccgg gcaggatgtg atcgcccagc taaagggcaa
    20161 agagctggga gaataccttc ttcttccggt aaatatgttc cgcagcggcg agaccgtttt
    20221 tttggatgat ctcacggccg aggacgcaga aaaggcttta caagtaaagg tgcgtatagt
    20281 aaaatcaagc gggcgcgatt ttgtaaaggc ggttctttat ccagagctgg aagcagaaca
    20341 aacggaggat gaagcaggcc ttggagcaga ctacggctat gagggctatg agcttgatga
    20401 gctgtcggac gagtcatata agtaggagga ataaagataa atgagcaaac cagtggtagc
    20461 catcgtagga agacccaatg tgggaaaatc tacgctgttt aatgtaattg caggagatac
    20521 catctccatt gtaaaggata ccccaggcgt taccagagac cgtatttatg cggactgttc
    20581 ctggctggat atgaatttca ctttaattga tacaggcggt attgaaccgg ataccagcga
    20641 tatcatttta tcccagatgc gggagcaggc ggagatcgcc atttccaccg cagatgtgat
    20701 cgtgtttatc gtagatgtgc gtcagggact ggtagatgcg gattccaagg tggcagatat
    20761 gctccgcaaa tccagaaaac cggttgtgct ggctgtaaat aaagtggaca gcatggcaaa
    20821 atttggaaat gacgtatatg agttttacaa tctgggaatc ggcgatcctg ttcctgtttc
    20881 tgcggcatcc cgtctgggga tcggtgatct gttggatgag gtggtaaaac attttgacgg
    20941 aactgcctca gaggaagagg aggatgaccg tccgagaatt gcggtggtcg gaaagcccaa
    21001 tgtgggaaaa tcttccatta tcaataagct tttaggagaa aacagggtca ttgtatctga
    21061 tattgccgga actaccagag atgcagtgga tacagagatt gtccacaacg gtacgcccta
    21121 tgtatttatc gacacagcgg gactccgccg caaaaataaa atcaaagaag aactggagcg
    21181 ctacagtatt atccgtacgg taaccgcagt ggagcgggcg gacattgtgg ttgtggtcat
    21241 tgatgccaag gaaggcgtta cggagcagga tgcaaagatc gccggaattg cacatgacag
    21301 aggcaaggga attattgtgg ctgtaaataa atgggatgcc attgagaaga atgacaagac
    21361 catttaccag tatacaaata aaatccggga aactctggca ttcatgccct atgcggaatt
    21421 tatctttatc tcggctgcca ccggacagcg tcttccaaag ctgtttgaca tgatcgatat
    21481 ggtgcgccag aaccagaatc tccgtgtagc gaccggtgtt cttaatgaga tcatgacaga
    21541 ggcagtagcc atgcagcagc cgccttccga taagggcaag cgcttaaaga tttactacat
    21601 gacccaggta tctgtaaagc cgccgacctt tgtggtgttt gtaaacgata aggaactgat
    21661 gcatttctcc tatacgagat atctggagaa tcagatccga aacgcttttg gatttaaggg
    21721 tacctcctta aaattcatca tccgggaacg gaaggaaaaa gatcagtaat acaaggggat
    21781 atgagaaatg gaacgcatca tttgtctggc cgccggttat ctgtgcggcc tctttcagac
    21841 cggatatatt tatggaaaac ttcacggcat tgatatacgc agttacggaa gcggcaactc
    21901 cggcagtacc aatgccctgc gtgtaatggg aaaaaaagca ggtcttacgg tttttgtggg
    21961 agatttctta aaaaccgtga tcccctgctt tatcacgcgg ctgctgttta aggatcagcc
    22021 ggagcttatg tatgtgtata tgctctatac gggccttgga gtgatcctgg gacataattt
    22081 ccctttttat ctaggattta agggaggaaa gggaattgca gccacagcgg gaattatttt
    22141 ctccattgac tggagactga ctgtgatctg cctatcctgc tttattctgc ttgtggcagt
    22201 gacacgctat gtatcgttag gttctttagt ggtatctgtg atccttcttg tctgggacgc
    22261 agtacagaat ctccgcggag cctatggcct gataccggga agactggagt ttacagctgt
    22321 gacagccgtg atcacggtca tggccttctg gcgccaccgg gcgaatatcg gccgtctttt
    22381 aaagggcacg gaaaataaag tgggagccaa aaagtagtaa atacataaat acagtgacag
    22441 gaggggtacg atatgtctaa gattggtgtg atcggttcag gaacctgggg aactgcttta
    22501 gcggttctcc ttacgggaaa cggccatgag gtggagctgt ggtctgcggt tcctgcggag
    22561 gtagaagcgc ttacagccac aagacgccat cccaatctgg gggatacgcc gatcccggag
    22621 aagatccgtg tgacaggaga tcttgagcag gctatgaagg ataaggagct tctggttctt
    22681 tcggttccat cggtttatgt gagggaaacg gcgcacagga tggctccctt tttaaaggaa
    22741 gggcaggtca ttaccaatgt agccaagggg attgaggata cgactcttaa aaatctgtct
    22801 gagatcattg aggaagagct tccggctgcc agggttacgg ttctttccgg gcccagccat
    22861 gcagaggagg taagcagggg actccctaca acctgcgtgg caggagcgca cagaagacag
    22921 gatgcggagc tggtgcagaa cctgtttatg agccctgtat tccgggtgta taccagtccg
    22981 gatatgctgg gaattgagct gggaggcgcg cttaaaaatg taatcgccct tgctgcgggg
    23041 attgcggacg gcttaggctg cggtgataac acaaaggctg cattgatcac cagaggcatt
    23101 gccgagatca ccaggcttgg tactgccatg ggaggaagcc cggagacctt ttccggcctg
    23161 actggaatcg gagatctgat tgttacctgc gccagtatgc acagccgaaa ccgcagagca
    23221 ggtatcctga tcggtaaggg atatacgatg gatgaagcca tgaaagaagt aaaaatggta
    23281 gtagagggtg tatactctgc cagagcggcc agggctcttt ctaagaaata tcaggtgtcc
    23341 atgccgatcg tggagcaggt aaatgaggtt ctttttgacg gaaagcctgc aaaggatgcg
    23401 ctgtatgatc tgatgctcag ggaccggcgg gcagaaaatg ctctgctgga atggaaggaa
    23461 taacttttcg ttatagcagg aatcagggaa aacggttgac aatgcagagg gaaattctta
    23521 taatcttaag aaagctttaa tacagcagag cattataata tgcagtgagc agtgttccgg
    23581 ctttaaagag ggcagggaca gtgtctggta ctgcaggagg aggatattat gaaaaaaaga
    23641 cttttatgcg caggacttgc ggcagcaatg gcagtttcat taaccgcctg cggaggttcg
    23701 gcagccggga caaaagctga gggaagttcc gcttcagcag aagcaaactc tgagggcgga
    23761 agtgaagcgg cgaaggtatt taagatcggc gggattggcc ctgttacagg cggcgcggct
    23821 gtatatggaa tggctgtaca gcacggtaca gagctggctg taaatgagat caatgcagca
    23881 ggcggtatca atggctatca gattgagttc cagttccagg atgatgaaca tgacgcggaa
    23941 aaaagtgtaa atgcgtataa taccttaaag gactggggaa tgcaggtact ggtaggaacc
    24001 accacatcag ccccatgtct tgcagtagcg gataaaacag cggctgataa tatgttccag
    24061 atcacgccat ccggttccgt tgttgaatgt gcagcgaatc cgaacgtatt ccgtatctgc
    24121 tactctgacc cggatcaggg tgcggcttct gctacttata ttggagagca taagcttgca
    24181 tctaagattg cggtaatcta tgacagctcg gacgtatatt ccagcggggt atatgagaag
    24241 tttgctcagg aagctccggc ccaggggatt gagatcgtag cagcagaggc attcacagcc
    24301 gacagcaaca aggatttctc cacgcagctt cagaaggcaa aggatgccgg cgctgagatg
    24361 gtattccttc cgatctatta cacagaggct tctctgatcc ttcagcaggc caataccatg
    24421 ggctttgcgc cccagttctt tggggtagac ggtatggacg gtatccttca ggtagagggc
    24481 tttgatacca gtctggcaga agggctgatg ctcttaactc catttgcagc tgatgcaaag
    24541 gatgacctca cagtaaagtt tgtaaaggca tatgaagatg cttacaaaga gacgccgatc
    24601 cagtttgcag cagacgctta cgatgcagtt tatgctgtaa aggcagccat tgaggatgca
    24661 aaggcaacac cggatatgtc tgtcagcgat ctgtgcgagg cgatgaaggc atccatgctg
    24721 aatatcaagc tggatggtct taccggcacc ggaatgacct ggacagagga cggcgagccg
    24781 cacaaggcac ctaaggctgt gaagatcgta gacggcgcat atgcggccat ggaataaatt
    24841 ttcataacga aagacccgaa agaggggtgt cgtaagacag ccctcttatc tgtagtaaac
    24901 catagaagag gtgttataga atgagctttg ttaactattt gatcaacggc gtcagcctgg
    24961 gcagcgtata cgcaatcata gcccttggat ataccatggt atatggaatc gccaagatgc
    25021 tgaattttgc tca
//
LOCUS       DBMDUH010000020        24937 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_1355_length_24937_cov_7.434128, whole genome shotgun sequence.
ACCESSION   DBMDUH010000020 DBMDUH010000000
VERSION     DBMDUH010000020.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 24937)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 24937)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24937
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1355_length_24937_cov_7.434128"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="ACZPCG_03215"
     CDS             <1..271
                     /locus_tag="ACZPCG_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008753840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HIR1284115.1"
                     /translation="LFPVLLNLVLMVGVDPIQFGVMVVLNLVLGLCTPPVGVCLFAAT
                     NIGETKLTGVVKELIPYLISNFIVLGLVTFVPFLTSGIAEMLLGA"
     gene            complement(373..1230)
                     /locus_tag="ACZPCG_03220"
     CDS             complement(373..1230)
                     /locus_tag="ACZPCG_03220"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005425161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR1284116.1"
                     /translation="MEQKTGLVLEGGGMRGIYTAGVLDLFMDQGVTFDGVIGVSAGAI
                     HGCSFLSGQRGRNIRYYKKYCQDPRFMSVRNLILTGDIAGERFCYHTLPEKLDPYDYT
                     AFNNNPAEFYVGCSNVETGKPEYLHLTDMKQQIDYLRASASLPLVSKIVHAGGKKLLD
                     GGCTDSIPVKAFMELGYPRNVVVLTRHRGYRKKAEGTRLTELCYRHYPAFVKTIKNRP
                     SVYNRTLDEIESLENSGQAFVIRPSRPLTIGRMEKDPQKLQLVYELGYQDAARCLLDL
                     LSWKQGPAR"
     gene            1461..1700
                     /locus_tag="ACZPCG_03225"
     CDS             1461..1700
                     /locus_tag="ACZPCG_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HIR1284117.1"
                     /translation="MKVTICIGSACHLKGSREIIEKLQKLVNENGLSEQVDLNGAFCS
                     GNCDHGVCVTVDGELYSLKPEDTEAFFENEIKGRL"
     gene            1703..3706
                     /locus_tag="ACZPCG_03230"
     CDS             1703..3706
                     /locus_tag="ACZPCG_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HIR1284118.1"
                     /translation="MGMEIIDFKATKCKHCYKCVRYCEVKAIQVKDGRAVIMPDKCIL
                     CGHCLKICPQSAKTLRSDLELVKGWIAAGERVVVSIAPSYMGLLKYKTIGQVRGALIR
                     LGFEDVRETSEGAAYVTAEYARLLKEHQMENIITTCCPSVNDLIEIYYPELVKYLAPV
                     VSPMIAHGRILKEELGREVKIVFLGPCIAKKKEANDPRHDNCIDAVLNFRDMKKWLDQ
                     EEISIEDCEDMPFTAFDPKVNRLYPVTNGVVNSVLAEEESRGDGYRKFYVHGETNCID
                     LCRSMARGEIKGCFIEMNMCAGGCIKGPTVDDEEFISRFKVKLDMEERICREPADRSQ
                     MEHAVEAVSFRKEFLDRSPKDPMPTEEQIRQILRMTNKFKPEDELNCGACGYPTCREK
                     AIAVFQHKAEVSMCIPYMHEKAESMSNLVMETSPNIVLIVGPDMRILEYSDVGEKYFG
                     KTRTEALEMYLYEFIDPVDFQWVFDTHQNIHGKRVSYPEYHLSTLQNIVYIEKENAVL
                     ATFIDITREEELAQEDYEKKLETIDLAQKVIHKQMMVAQEIAGLLGETTAETKTTLSK
                     LCRSLLDDSSLHEEDEMEPPLENVHIGSGAVPLSGVMTRDSLGGVSPRQAGAQAVISG
                     APGIRPGASGASASKADSSETAEKPAGYVHLGSIRPKSPGFGR"
     gene            3710..4888
                     /locus_tag="ACZPCG_03235"
     CDS             3710..4888
                     /locus_tag="ACZPCG_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HIR1284119.1"
                     /translation="MEITVDVAYKSLNKYSEILCGDKVEILQTEDSNIMILADGMGSG
                     VKANILSTLTSKILGTMFLNGATLEECVDTISETLPICQVRQVAYSTFSILQVFHNGD
                     AYLVEYDNPGCIFIRDGKLMEIPQNIRELQGKRINEYRFKVQKGDALILMSDGTIHAG
                     VGDLLNFGWLWKDIADYAVRQYQITVSAMRLAASISRACDELYQFHPGDDTTVACMRI
                     INSKPVHLMTGPARNPEDDTAMVEDFMSDPNAATIVCGGTSATIVSRVLNRKLDVSLD
                     YVDPEIPPIAYMDGVDLVTEGVLTLNRVLQLLRRYVKNETVSEDFFEELDKPNGGSMV
                     AKILIEDCTEVHLYVGKAINSAYQNPGLPFDLGIRQNLVEQLRHLIEDMGKKVVITYY
                     "
     gene            4983..6434
                     /locus_tag="ACZPCG_03240"
     CDS             4983..6434
                     /locus_tag="ACZPCG_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR1284120.1"
                     /translation="MVTNDATILRLKHDVLYEVAKAAWEGNLEEKRDEIPYDMIPGPQ
                     ASYRCCIYKEREIIKQRVRLAEGKCPTGRDSSNVVQVINAACEECPIASYIVTDNCRK
                     CMGKACQNSCNFGAITMGHDHAYIDPNKCKECGKCAQACPYNAIAHLERPCKKVCPVD
                     AITYDEYGICVIDEKKCIQCGACIHSCPFGAIGSKTFMVDVIRLIKAGKKIVAMVAPA
                     TEGQFGSDITMESWRVALKQMGFADMVEVALGGDMTAAYEAEEWAEAYKEGKKMTTSC
                     CPAFVNMIKQHFPMLLDNMSTTVSPMCAVSRLLKSQDHDVVTVFIGPCIAKKSESLDL
                     NIEGNADYVMTFGEIRAMMRAKGVELQPATNEYQEGSVFGKRFGNGGGVTAAVVECMK
                     EREENFDAKVLRCNGGAECKKALLLMKVGRLPEDFVEGMVCAGGCVGGPSKHKGETEA
                     KKARDQLIGEADDRRILENLKKYPMDQFSMHRH"
     gene            6515..7207
                     /gene="cobI"
                     /locus_tag="ACZPCG_03245"
     CDS             6515..7207
                     /gene="cobI"
                     /locus_tag="ACZPCG_03245"
                     /EC_number="2.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030788 - precorrin-2
                     C20-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-2 C(20)-methyltransferase"
                     /protein_id="HIR1284121.1"
                     /translation="MHGILYGVGVGPGDPRLMTFLAADTIKKCEVLAVPARTREQAVS
                     YQIARGLLPELDQKACLSLAVPMTKDRTVLNAAYEQAADLVEEQLKAGKDVACLTLGD
                     PTIYSTYIYLHRIIKARGYETQIISGVPSFCAASARLGDSLADRSEQLHIIPSAYGVE
                     EALALPGTKVLMKAASKMDAVKKTLLEQKAGAVMVENCGLENETIYRGTEQIPDQAGY
                     YSLIVVKEQQKW"
     gene            7401..9380
                     /locus_tag="ACZPCG_03250"
     CDS             7401..9380
                     /locus_tag="ACZPCG_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1284122.1"
                     /translation="MRKNAAKIAALMLGAAMALTACGGGQSTGNGGGNTSAGGNAGSG
                     SSTQAEESDKNAIKDLVTYEAPNREQEGFFILNTEKAADLNILCNLYAPLIEVDENGK
                     LRPAVAKEWGTEDGGLTWTFKLRDDVKWVDINGNVKADCTAQDWITAMEWVLNFHKNG
                     SNNTSMPLALIKGAEDYYNYTRELSAEEAKALDTSKMLEMVGIEAPDDYTLIYHCALN
                     APYFDTVCTSACMYPISQALIDELGVDNMVGMTNETMWYTGPYFETTFTMNNEKILTK
                     NENYFDKDAKLFDTVTVRMVDGTQLYQLWQNGELDNIALAEADLRTIYNDDSNEWKPY
                     LSETRPKKYSYQMHFNFDKFKDDGSADENWNKAVANEAFRLSLYYGLDLRNYWSRTNF
                     IEPLHCENVGYTMKGLLYYSDGTDYAAKVEEKMGFAPSDGENPRRFDAAKAEAYKKQA
                     MEELAAKGVSFPVEMDYYIIAGNQDALDKATVLKQTFAECLGAEYVNLNIKTFVSSQT
                     KEVVQPGLGSFYISGWGADYGDPANFIDQERYGYDGAYYSNNYSRINNATDEDLIATY
                     KEFSALAEAAGAIYDEPDKRYDAYVDAEVYLLQHALTIPMEYEIQWQLTKINDYSKMN
                     AMYGIQNYTYKNWKTSVDAYTTEQYKEFQAASEAK"
     gene            9384..10334
                     /locus_tag="ACZPCG_03255"
     CDS             9384..10334
                     /locus_tag="ACZPCG_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1284123.1"
                     /translation="MVKYTAKRLLQSLITVLVVATIVFLLMRMLPTDYYFTEDQVMKL
                     TEEQKNAQLEAAGLLDPMEQQLGRFYGQLLRLDFGTSRRIQNGAPVLKVIGTKFSVSM
                     RLGCIALVIALLIGVTMGIIQSRNKDRMLDHVGTAYTVFVNAVPPLVSYSLVLAFGAN
                     LLNLPALYSTRNATRSSILPIVCLALTSIAHYALWTRRYMVDELNKDYIKLARIKGMS
                     SKNIMVKHVLKNAFVPLVQYIPASFLLTISGSLLVERFFSVPGMGPLLTDAITRYDLN
                     VVQTLVIIYAALGIIGVFLGDILMMTLDPRIKLTDKGGSR"
     gene            10334..11263
                     /gene="oppC"
                     /locus_tag="ACZPCG_03260"
     CDS             10334..11263
                     /gene="oppC"
                     /locus_tag="ACZPCG_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016441436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HIR1284124.1"
                     /translation="MNNDTQKDKFRFVEYSADAAERIGYSDYSYWKSVFQNFFKKKSA
                     VVMAGVFFALVIFSFIALSIGKYNYSEAQIMANSSLAFTKPNSEFWFGTDNLGRDYWC
                     QVWYAAQVSIKLALIVAAGECVIGVIIGCIWGYVRSLDRIFTEIYNVINNIPIIIYMT
                     LAALVFGQSFRNMALAMIAFGWLVMARNVRNLVLMYRDREYNLASRCLGTPVWRILLK
                     NIFPYLISVVILRLALSIPQTIALESTLSYLGLGLDVNTPSLGILLRNARNYFLQYPY
                     LLVFPAVIVSLITITFYLVGNAFSDAADPRNHV"
     gene            11289..12329
                     /locus_tag="ACZPCG_03265"
     CDS             11289..12329
                     /locus_tag="ACZPCG_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1284125.1"
                     /translation="MEREPILSARDVEISFSLRGKKLNAIRRCSLDLYQGETLAIVGE
                     SGSGKSVFTKSFLGMLDANGSITGGSILFEGKDLAKYKTEKDWMTIRGKKISMVMQDP
                     MTSLNPLKKIGRQIEESIELNQGIKGPEAKKMALEMLKKVGIPDPERRYQQYPHEFSG
                     GMRQRVVIAIAAACHPQILICDEPTTALDVTIQAQILNLIRDLQKELNMTVIYITHDL
                     GVVANVADRVAVMYAGQIVEYGKVDEIFYDAWHPYTWALLSALPQLGIKGEELPTIEG
                     TPPNLFNEIKGDAFAPRNRMALAIDFEEEPPFFDVSPTHKAKTWYLDPRAPKIEQPDS
                     IRKLREKMKGRG"
     gene            12336..13271
                     /locus_tag="ACZPCG_03270"
     CDS             12336..13271
                     /locus_tag="ACZPCG_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1284126.1"
                     /translation="MAEKEKLIEIKDLQITFGSGKRKFVAVDHVNFDIYKGETFSLVG
                     ESGSGKTTIGRAITRINPVSGGEILYRGERISGKISKELDRKVVKSCQMIFQDPMASL
                     NERAKVDYIVSEGLINFHLYKDETDRKRKVEHALREVGLLPEFASRFPHEFSGGQRQR
                     IGIARALIMEPEFVVADEPISALDVSIRAQVLNLLNHLKQTRGLTYLFIAHDLSVVRF
                     ISDRIAVIHKGRIVELAEAEELFLHPFHPYTKALLSAVPIPDPELEKKKKLLVYDPSI
                     HDYSTDKPVWEEILPGHFVWGNQRELEGYRKEIEG"
     gene            13278..13703
                     /locus_tag="ACZPCG_03275"
     CDS             13278..13703
                     /locus_tag="ACZPCG_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284127.1"
                     /translation="MNKKGSLFKTYMIRPVLYKCVTRAALAVTALLLWDRFINTAGRF
                     SLFRDGCLTAGLLFFCMAWFSYLHLDGVTIHHMLEERKKKKPVRKSYTDIVDFADEHI
                     VSFDELSGEERAVCSMSANLICGALFMLPWLVKFLGDFL"
     gene            13837..15456
                     /locus_tag="ACZPCG_03280"
     CDS             13837..15456
                     /locus_tag="ACZPCG_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005330295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1284128.1"
                     /translation="MKKRFAAAVCLIATALSLSACGGSGQTETTAAGGAAESTTAAPA
                     AGVPAGGKVVRVAAVDPQVALDPQQYTYSIVMKITDNVTESLITTMEDGSLEPNLLTA
                     LPEVSEDGMTYSFELLPDVKFHNGETLKASDVKYSFERLVKLTKMATLMENVEGYKEL
                     ADGAAEEMSGIQVQDDTHFTIKLVKPYAPFASVLSTGYCAIYPEAACEEAGDTWGMQV
                     LYGTGPFKMDSYQSGVGVVLSRFDDYHGGAVKLDGLDYKFIEDVNTQVLEYQKGNVDY
                     VDVDPSIYPVYSSNPQIKDQMHGFQPTGNYTLNLNAKTFPDPKVRQAIALSIDRKSIC
                     ESILHGTATVPTSYIPAGIIGHDETLPEFEYNPEQAKALLAEAGYPDGIDLRVTVNTK
                     YQGNVAIVTAFQQQAKAAGIRVEVEQVDSAAWSDMKKNGGVDCGISNWYVDYSDPESM
                     LYPMTKTDSNSSFWHNAEFDQLLEDGIATTDPAERQEIYAKAEHIMTREDWATTPLYN
                     ETKFYLLNPKITGFKMNATFRMFWKNADIQQ"
     gene            15531..16463
                     /locus_tag="ACZPCG_03285"
     CDS             15531..16463
                     /locus_tag="ACZPCG_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005330296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1284129.1"
                     /translation="MLSYTAKRLAQTIFVLLGISLITFILLQVVPGDPVAMMLEKRAD
                     PETIAKVRHELGLDLPYHVQYLNFLKGAVRFDFGTSYFTKEVVSDALFRCFKVTVKLA
                     CMSFAFAAVIGIPCGIFSAVQRGKGIDTAVMILSIVGVSAPAFWVAIILQIVFGLKLN
                     ILPISGFDSAASYILPSVALGARYAGSMARITRTSMLEVLNQDYIRTARAKGAKRWSV
                     ILKHALKNAMIPIVTLVGTDFGYMLTGSMLIEKVFSIPGIGKLAVDAMSNRDLPLLEG
                     TVMYIAFVFVIVNLLVDISYAFLDPRIRYGKGAA"
     gene            16471..17379
                     /locus_tag="ACZPCG_03290"
     CDS             16471..17379
                     /locus_tag="ACZPCG_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005330297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1284130.1"
                     /translation="MIRFFNKQKQFEQDVMEQGMQYSSGFYGDSLRRLKKNKMAMFCA
                     GILVLLVLVAIFAPVIAPYDPTYQDYSAVLAEPSMAHPFGTDEYGRDILSRIIYGSRV
                     SLSIGIVAQVVASLVGVTLGAVAGYYGGRIDSVISRIMEIFQAFPDLIFAMAIMTFLG
                     KGVLNLFIALGLLTWVRTARMIRGSVMQLKEKEYIEASRACGASTFWIITKGLVPNCL
                     STIIVLVTLGIPNAIMYEASLSFLGIGIQPPTPSWGNMISAAQTFISYRPVYSIVPGV
                     AIMITVIAFNIFGDGLRDALDPKLKN"
     gene            17394..18386
                     /locus_tag="ACZPCG_03295"
     CDS             17394..18386
                     /locus_tag="ACZPCG_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1284131.1"
                     /translation="MADKILEIKNLKTYFRTDAGLVKAVNDVTFSVERGKTLGIVGES
                     GCGKSITSLSIMGLVETPPGIHAGGEILFEGEDLLKKNEDQMRQIRGNKVAMIFQEPM
                     TSLNPVFTIGQQLMEAILLHENVTKQQAREKGIEMLKKVKIPLAEKRFDEYPHQLSGG
                     MRQRVMIAMALCCNPQLLICDEPTTALDVTIQAQILDLINELKEETGTSVMMITHDLG
                     VIAEVADDVMVMYAGKVVEHATCDQIFDEPLHPYTAGLMNCIPRLDGDDTKELSVIEG
                     MVPSFDDMPEGCAFCPRCPYAKEICRKQMPELEEVNGRRVRCFKYMKGWEESAK"
     gene            18383..19381
                     /locus_tag="ACZPCG_03300"
     CDS             18383..19381
                     /locus_tag="ACZPCG_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005330307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1284132.1"
                     /translation="MSEHINETPLLEVKHLRKLFPVKQTFRSGEKTFIKAVDDVSFTL
                     NPKETLGVVGESGCGKSTMGRSVLRLIEPTDGEIIYKGQDFKKASGQELRKMRAEMQI
                     IFQDPYASLNPRMTIGEIIEEPLKIQKRFASKEEMRAHVLEVMEVVGLNPKYYNRYPH
                     EFSGGQRQRIGIARAIVLGPSLVVCDEPVSALDVSIQSQVLNLLRHLQESMGMAYIFI
                     SHNLSVIRHISDRVMVMYLGHVVELADKHQLYDNPSHPYTQALLSAIPMPERHSTRKK
                     ILLQGDLPSPADPPSGCVFHTRCFMAQEICSVQKPELKDIGGGHMCACHFCGGCQK"
     gene            19463..20548
                     /locus_tag="ACZPCG_03305"
     CDS             19463..20548
                     /locus_tag="ACZPCG_03305"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HIR1284133.1"
                     /translation="MKITDMKVHMMQVPLVRPFRISLGVITHAVSCLVEIETDAGITG
                     YGEGSPGPLITGENLPGTVETLRVFREKLIGMDPRDIEGINAVMNRAVAYAGTAKAAV
                     DIACHDILGKAAGLPLYKLLGGLSNTIETDMTVGIDTPELMAAAAKEHVAAGFRVIKT
                     KVGTNIADDIARVKAIREAVGDDVKIRLDANQGWHAREAVELLKRLNEYDIELVEQPV
                     RRFDFEGLKYVTANSPVPVMADESCWDSKDALRLVSERAVDFINIKLMKCGGLWEAKK
                     IVNIAEAAGVEIMLGCMAEESGIAINAAAALGAALGNITRADLDASFSLKALPYEGGF
                     TIENTKDLVLSENPGLGLVRLKEEMFQ"
     gene            20545..21963
                     /locus_tag="ACZPCG_03310"
     CDS             20545..21963
                     /locus_tag="ACZPCG_03310"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR1284134.1"
                     /translation="MSRAVTEEEMTQGISRLEAHTAQIMEEAKARGIAVGVVDRTGRI
                     LYEKYFGYRDEEKKLPINRDTIFGMASVTKSFTALSIMQMQKDGILSVDDAVSAYIPE
                     FTNKNQKEPVRLSHLMSHSGGYFPLPRIVVDKTAQEMGIKDSLEEELVDREDFAEEGI
                     RRVAGRLDSQTHFTGRPGQRMSYCNDGFGLLSDIVRRHSGYGSFSAYLKERIFKPLGM
                     ERSNISFLRNSLDENAATLYTLEDGTWRADHDYENDAFVLHGGGALKSTLGDMLKYVS
                     MYLNEGKSQDGTQVIDRDSIEEMCRPRQIVKPGVYYGYGLEMSQMEDMVLVEHGGSLP
                     GVSSNLCFCPQKGIGVIVLCNTMDVPVYSIGTMALRLYCGLPLEAERIEHMPRSWSED
                     EKRELAGDYISGEGDRFSIKELENGELAMKVNGTPIEMKSVYPWQGMVRKKYSDVYLT
                     AFRDENGTVFAARYGSRIFPKA"
     gene            22200..24134
                     /locus_tag="ACZPCG_03315"
     CDS             22200..24134
                     /locus_tag="ACZPCG_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002600777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1284135.1"
                     /translation="MKYQITDGTVSLGGETVLSHIDFEIQGAQKIAVVGRNGAGKTTL
                     LRLIAGELELERDDRRQGPGICCSRKLTIGMLGQQALLHEERTVEEYLMSRCPAEGLF
                     DRERFEYEREYDCLFTGLGFKKEDKKRPLSTFSGGERTKAALAGLLLEKPDILLLDEP
                     TNHLDMETVQWLEGYLRNYDRAVVMVSHDRFFLDRTAQIVYELEDRKLTKYPGNYTAY
                     REKKRKDYELQKKAYIRQQEEIARQEELIRRFKNKPAKASFARSRKKMLERMERVEAP
                     REDTAHIFTGEILPAVPGSKWVLEAEHLKIGYEGHPLLELSLRMRKGQKIALLGENGA
                     GKTTFLKTAAGSLPPVDGVCSLGNNITIGYFDQYSSAIESEQTVAEHFSELFPSLNQK
                     EVRTILGSYLFPGKEASRKVSSLSGGEKARLVLAELLQSRPNFLILDEPTNHMDIQAR
                     ETLESAFQAYQGTILFVSHDRYFIRQVAKSVLIFEGARAMYYPFGYEHYLERLEKEKE
                     GLSPAARIQAEEQALIAGLKAVPRAERHRLREIPTEEAYDQWRLKMAEDAMNEAAGQA
                     EEQMKVWEENRLKRLWEQAEEQPASALEAQPLEDIAPLWEKWHQACMDWYEIWSELHP
                     EPEAPEPEKFADLESKEDAE"
     gene            24131..>24937
                     /locus_tag="ACZPCG_03320"
     CDS             24131..>24937
                     /locus_tag="ACZPCG_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR1284136.1"
                     /translation="MILIKNADVYAPEHLGIKDVLICGAQIEAVGEGLEGGSGCRIID
                     AEGKKLTPGLIDRHVHVTGGGGEGSFHTRTPQIQLSSVIRAGITTLVGLLGTDDVSRT
                     PEDLIAKVKALKEEGISAYALCGAYGVPSPAITGSIKKDIAFIDEILGLKLAVSDHRA
                     PNISTEELIRLASDVRTAGMISGKCGCVVLHMGDGKKMLSQVFEALEETEIPVKTFQP
                     THVNRNHELFLDSLKLAKLGGTIDLTCEDFPHGLIEDGKDPVIGLLEEARR"
BASE COUNT         6605 a         5485 c         6754 g         6093 t
ORIGIN      
        1 tctgtttcca gttcttttaa atctggttct tatggtaggc gtagatccaa ttcagttcgg
       61 tgttatggtt gtattgaatc tggttcttgg tctgtgtact cctccggtgg gggtatgttt
      121 gtttgcagcc accaatattg gtgagacaaa gctgacagga gttgttaagg aattgattcc
      181 ctatctgatt tccaatttca tcgtattagg acttgttaca tttgtaccgt tcctgacatc
      241 tggtattgca gagatgcttc tgggcgctta aggctgtgca gttgaaaatg tatataaaaa
      301 tcactggatt gatttttcga cccgctgatt ttttgataaa atatgaaaaa gaaagatatg
      361 aacagggacc ggtcatctgg ccggtccctg tttccaggat aaaaggtcta aaaggcatct
      421 ggctgcgtcc tgatacccaa gctcgtaaac aagctgaagc ttttgcggat ccttttccat
      481 tcgtccgatg gtaagaggcc tgctggggcg gatcacaaag gcctggccgc tgttttccag
      541 agactcgatc tcatccagcg tccggttgta cacggatggc cggtttttaa tggtttttac
      601 aaaggctgga taatggcggt agcacagttc ggtaagccgg gttccctctg cttttttccg
      661 gtaacccctg tggcgggtaa ggacaacaac attccttggg tatccaagct ccataaaggc
      721 ttttaccggg atgctgtccg tacagccgcc gtctagcagc tttttccctc cggcatgtac
      781 gatttttgat accagaggca gtgatgcaga ggcccgcaga tagtcgatct gctgtttcat
      841 gtcggtcaga tgcagatact ccggttttcc tgtttctaca ttgctgcagc ctacataaaa
      901 ttcggccgga ttattgttaa aggctgtata gtcataagga tccagctttt ccggcagcgt
      961 gtggtagcag aaccgttctc ctgcgatgtc tccggtgagg ataaggtttc taacgctcat
     1021 aaagcgagga tcctggcagt attttttata atatcggatg ttccgcccgc gctgtccgga
     1081 aagaaagctg cagccgtgga tggctcctgc ggatactccg atcactccgt caaaggttac
     1141 tccctgatcc ataaagagat ccagcacgcc ggctgtatag atcccgcgca ttccgccgcc
     1201 ttccagcaca agtcctgtct tttgttccat tttctgattc ctccatttgg ctttgccggg
     1261 cggttctgtc cggcaggcat atcgtttgaa atatatccag tataccacac ttttccatcg
     1321 gaaaaccttt gtttttgcag aaatattatt gataaaaata tgacaatatg agataatggc
     1381 attacatgtg gggagacccc tgtataaagg ataaagaaca tacagacaga gtgaaacgaa
     1441 taacggaggg tgaagtgaaa atgaaagtaa caatatgtat tggaagcgcc tgccatttaa
     1501 agggttccag agagattatt gaaaagcttc agaagcttgt aaatgaaaac ggcctgtcgg
     1561 aacaggttga cttaaacgga gctttctgct ccggaaactg cgaccacggc gtatgtgtga
     1621 ctgtggacgg agagttatat tcattgaagc cggaggacac agaagcgttt tttgaaaacg
     1681 agatcaaagg gagactgtga ttatgggcat ggaaatcatt gatttcaagg ccacaaaatg
     1741 taagcactgc tataaatgcg tccgctactg cgaggtaaag gccatccagg tcaaggatgg
     1801 acgagccgtg atcatgcctg ataaatgtat tctctgcggc cactgcctga agatatgtcc
     1861 gcagtctgca aagacgctta gaagcgatct ggagctggtg aagggctgga tcgcagccgg
     1921 cgagagggta gtggtttcca ttgcaccctc ttatatgggt ctgttaaaat ataagaccat
     1981 cggccaggtc cgtggggcac tgatccgtct ggggtttgag gatgtgaggg aaacatccga
     2041 gggcgctgcc tatgtgacgg cagagtatgc cagactgctt aaggagcatc agatggaaaa
     2101 tatcattact acctgctgcc ccagtgtcaa tgacctgatc gagatttact atccggagct
     2161 ggtaaaatac cttgcgccgg tggtatctcc catgatcgcc cacggacgca ttttaaagga
     2221 agaactgggg cgtgaggtga agatagtatt tttaggcccc tgtattgcaa aaaagaagga
     2281 ggcaaacgat cctcgccatg ataactgcat tgatgcggtc ttgaatttcc gggatatgaa
     2341 aaaatggctg gatcaggagg aaatttccat tgaggattgt gaggatatgc cctttacggc
     2401 ctttgatccc aaggtaaacc gcctgtatcc tgttacaaac ggagtggtaa attcggtact
     2461 ggctgaggaa gaaagcaggg gagacggcta ccgcaagttc tatgtccatg gggagacaaa
     2521 ctgtattgac ctgtgcagga gcatggccag gggagagatc aaagggtgct ttattgaaat
     2581 gaatatgtgc gccggcggct gtattaaggg gccgacggtg gatgatgagg agtttatttc
     2641 acgctttaaa gtaaagctgg atatggagga acggatctgc agagagccgg ccgaccgttc
     2701 ccagatggag catgcggttg aggcagtttc tttcagaaag gagtttttag accgttcgcc
     2761 caaagatcct atgccgacgg aggagcagat ccgccagatc ctgcgcatga cgaataaatt
     2821 taagccagag gatgagttaa actgcggggc ctgcggttat cctacctgtc gggaaaaggc
     2881 gatcgccgta ttccagcaca aggcagaggt ttccatgtgc attccctata tgcatgaaaa
     2941 ggcggaatcc atgtctaatc tggtaatgga aacttcgcct aatattgtgc ttattgtagg
     3001 gccggatatg cggattttag agtattccga tgtgggagag aaatactttg gaaagacacg
     3061 cacagaggct cttgagatgt atctgtatga attcattgat ccggtggatt tccagtgggt
     3121 attcgacacc catcagaata tccatggaaa gcgggtcagc tatccggagt accatttgtc
     3181 cacactccag aacattgtat acatagaaaa ggaaaatgca gtgcttgcta cctttattga
     3241 tatcacccgt gaggaggaac tggctcagga ggattatgag aagaagctgg aaactattga
     3301 tctggctcag aaggtgatcc acaagcagat gatggtggct caggagattg caggccttct
     3361 gggagaaacg acggcggaaa ccaaaactac cttaagcaaa ctgtgccgtt ctctgttaga
     3421 tgattcctct ctccatgagg aggatgaaat ggaaccgcct ttggaaaatg tccatatagg
     3481 aagcggggcg gttccactaa gcggtgtgat gacaagggat tccctgggag gcgtctctcc
     3541 aaggcaggca ggagcacagg ctgtcatttc gggagcgccg ggtatacggc cgggagcatc
     3601 gggagcatcc gcctccaagg cggacagctc agaaacggca gaaaagccgg cgggctatgt
     3661 ccatctgggg agcatccggc ccaagtctcc gggctttggg aggtagcgta tggagataac
     3721 ggtagatgtt gcttacaaaa gcctgaacaa atacagtgag atcctctgcg gcgacaaggt
     3781 ggaaatcctt cagacagagg attccaacat tatgattctg gccgacggta tgggaagcgg
     3841 ggtcaaggcc aatattttat ctactcttac ctcaaaaatc ctgggaacca tgtttttaaa
     3901 cggagctacc ctggaggaat gtgtggatac catcagtgag acccttccca tctgccaggt
     3961 gcgccaggtg gcctactcta ctttcagcat tttgcaggtt ttccataatg gagacgccta
     4021 tctggtagag tatgacaatc cgggctgcat ttttatccgg gacggaaaac tgatggaaat
     4081 tccccagaac atccgggaac ttcagggaaa acggatcaat gaataccgtt ttaaggtgca
     4141 gaagggagac gctttaatcc ttatgagcga cggcaccatt catgcgggag ttggcgatct
     4201 tttaaacttt ggctggctgt ggaaggatat agcggactat gcggtgcgcc agtatcagat
     4261 caccgtttcc gccatgcgtc tggctgcatc catcagccgt gcctgtgacg agctgtacca
     4321 gtttcatccg ggagacgata ccacagtggc ctgtatgcgc atcataaaca gcaagccggt
     4381 tcatctgatg actggtccgg cccgaaatcc agaggatgat acagccatgg tagaggattt
     4441 tatgtccgat cccaatgccg cgaccattgt atgcggcggc accagcgcta ccattgtgtc
     4501 acgggttctg aaccggaagc tggatgtatc gctggactat gtggatccgg agattccgcc
     4561 cattgcctat atggacgggg tagatctggt gacagaaggc gtccttacgc tgaaccgcgt
     4621 gctgcagctt ttgcggcggt atgtaaagaa tgaaaccgta tcggaggatt tttttgagga
     4681 actggataag cccaatggcg gctccatggt ggcaaagatc ctgatcgagg actgtacgga
     4741 ggttcatctg tatgtgggaa aggccatcaa cagtgcctat cagaatccgg gtcttccttt
     4801 tgatctggga atccgccaga atctggtgga gcagctgcgc catctgatcg aggatatggg
     4861 aaaaaaggtg gttatcacct attattaagt tggcaatgga ccctttgggc cttccaagat
     4921 atatatttgc tgtctggacg cttagaacag gcgtccggga ttatgaatgg aggaaatgaa
     4981 aaatggtaac aaatgatgca acgatcctgc gcctgaaaca tgacgtgctc tatgaggtcg
     5041 cgaaagctgc atgggaggga aatctggagg aaaaaaggga tgagatcccc tatgatatga
     5101 ttccgggccc tcaggccagc taccgctgct gtatttacaa ggagcgtgag atcatcaagc
     5161 agagagtccg tctggcagaa ggcaagtgcc ctacaggaag agacagttcc aatgtggttc
     5221 aggttattaa cgcagcctgc gaggagtgtc ctattgcgtc ctatattgtc acagataact
     5281 gccgcaagtg tatgggaaag gcctgccaga attcctgtaa ttttggagcc atcaccatgg
     5341 gtcacgacca tgcctatatt gatccaaata agtgcaagga gtgcggcaag tgcgcacagg
     5401 cctgtcctta taatgcgatc gcccatctgg agcgcccatg caaaaaggtc tgtccggtag
     5461 atgcgatcac atatgacgag tatggcatct gtgtgatcga tgaaaagaag tgtatccagt
     5521 gcggcgcctg tatccacagc tgcccctttg gagctattgg ttccaagacc tttatggtag
     5581 atgtgatccg tctgatcaag gcaggcaaga agatagtcgc catggtggcg cccgcgacgg
     5641 aaggacagtt cggatcggat atcaccatgg agagctggcg cgtggcctta aagcagatgg
     5701 gctttgcaga catggttgaa gtggctctgg gcggcgacat gacggcagct tatgaggcag
     5761 aggagtgggc agaggcctac aaagagggca agaagatgac tacatcctgc tgccccgcat
     5821 ttgtaaatat gatcaagcag catttcccta tgcttcttga caatatgtca actaccgtat
     5881 cacctatgtg cgccgtatcc agactgttaa agagccagga tcatgatgta gtgactgtat
     5941 ttattggccc gtgtattgcg aaaaagagtg agagcctgga tctgaatatt gagggaaatg
     6001 cagattatgt tatgaccttt ggcgagatcc gcgccatgat gcgcgccaag ggtgtagagc
     6061 tgcagcccgc aacgaacgag tatcaggaag gctctgtatt cggaaagcgt tttggaaacg
     6121 gcggaggcgt aacggcggct gttgtagagt gcatgaagga gagagaagaa aactttgacg
     6181 caaaggtact tcgctgcaat ggaggagccg agtgtaaaaa ggcgcttctt ctcatgaagg
     6241 taggccgtct gcctgaggac tttgtcgagg gcatggtctg cgcagggggc tgtgtcggcg
     6301 gccccagcaa gcataagggt gagacagagg caaagaaggc cagagaccag ctgatcggag
     6361 aggcagatga cagaagaatt ctggaaaatc tgaagaaata tcccatggat cagttctcta
     6421 tgcacagaca ttaaaaagaa atccgggagg cggatgtgct tcccggattt ctttttaaaa
     6481 gaataaaaac aagagaaagg aagaatgatt atttatgcat ggaattttat acggcgtagg
     6541 cgtaggaccg ggagatccaa ggcttatgac ctttcttgcg gcagacacta taaaaaaatg
     6601 tgaggttctg gctgtccctg cccggacaag agagcaggca gtgagttacc agattgccag
     6661 agggcttctg cctgagctgg atcaaaaggc gtgtctttct ctcgcagttc ccatgacaaa
     6721 ggacaggacg gttttaaacg cagcctatga gcaggccgca gatctggtag aggagcagtt
     6781 aaaagcagga aaggacgtag cctgcctgac cctgggcgat cctacaatat acagcaccta
     6841 tatttatctt caccgtatca taaaggccag aggctacgaa acacagatca taagcggcgt
     6901 tccctccttt tgtgcggcca gcgcccgtct cggagacagc ctcgcggatc ggtctgagca
     6961 gcttcatatc atcccctccg cctatggcgt ggaggaggct cttgctcttc ccggtacaaa
     7021 ggtgcttatg aaggcagcct ctaaaatgga cgcggtgaaa aaaacgctgc tggaacagaa
     7081 agcaggggca gttatggtgg aaaactgcgg attggaaaat gaaacgattt acaggggaac
     7141 ggagcaaatt ccggaccagg cagggtatta ttcgcttatt gtggtaaaag aacaacaaaa
     7201 atggtaaaaa aggccggttt tcgtgaatat gtctgtgatg gtaaatagta cgcggtcaaa
     7261 tgtacgccgg tgcttgacac aagacggtgt ttaatgataa aatacttcac tagatggcag
     7321 agggtcacac tgttttggac agtttttgaa cacggctcct tgtctttttg tcttttatct
     7381 atgaaaggag aatatgcact atgagaaaaa atgcagcaaa aattgcagct cttatgctgg
     7441 gcgcagcgat ggctcttaca gcctgcggcg gcggacagag cactggaaat ggtgggggaa
     7501 acaccagtgc cggaggtaat gcaggttctg gaagcagtac tcaggcagag gaaagtgata
     7561 agaatgcaat taaggatctg gtaacctatg aagctccaaa tagagagcag gaaggatttt
     7621 ttattctgaa tacagagaag gcagctgact taaatatact ctgtaatctc tatgcaccac
     7681 ttatcgaggt agatgaaaac ggtaaattaa gaccggcagt tgccaaggaa tggggaacgg
     7741 aggacggcgg ccttacctgg acctttaagc tgagagacga tgttaagtgg gttgatatca
     7801 acggcaatgt aaaggctgac tgtacagctc aggactggat taccgcaatg gagtgggtat
     7861 taaacttcca caagaacgga tccaacaaca catcgatgcc tttggctctg attaaggggg
     7921 ctgaggatta ttacaattat accagggagc tttccgcaga agaggccaag gctcttgata
     7981 catccaagat gctggagatg gtaggcattg aggctccgga tgattatacc ctgatctatc
     8041 actgtgcgct caacgctcct tactttgata ccgtatgtac atcagcctgc atgtatccta
     8101 tctcccaggc tctgattgat gagctgggcg ttgataacat ggtcggcatg acaaacgaaa
     8161 ccatgtggta tacaggtccc tattttgaga caacctttac catgaacaac gaaaagatcc
     8221 ttacaaagaa tgaaaattat tttgataagg atgcaaagct gtttgataca gttaccgtcc
     8281 gtatggtaga cggcactcag ttatatcagc tgtggcagaa cggcgagctg gacaacatcg
     8341 ctctggcaga ggctgactta agaacgattt acaatgatga cagcaatgaa tggaagccct
     8401 atctgagcga gacaagaccg aaaaagtatt cctaccagat gcactttaat tttgacaagt
     8461 tcaaggatga cggttcagca gatgaaaact ggaacaaggc agttgcaaac gaagccttcc
     8521 gcctttcttt atactacgga ctggatctga gaaactactg gtcaagaacc aactttatcg
     8581 agcctcttca ctgcgagaat gtgggctaca ctatgaaggg tctcctgtat tactctgacg
     8641 gcaccgatta tgctgcaaag gtagaggaga agatgggatt tgccccttct gacggcgaga
     8701 acccaagacg tttcgatgct gcaaaggcag aagcgtataa gaagcaggcg atggaagaac
     8761 tggctgccaa gggcgtttcc ttcccggtag agatggatta ctacatcatc gcaggcaatc
     8821 aggatgctct tgataaggca accgtattaa agcagacctt cgctgagtgc ctgggagcag
     8881 agtatgtgaa cttaaacatc aaaacctttg tttccagtca gaccaaggag gtcgtacagc
     8941 ctggtctggg ctccttctat atcagcggct ggggagctga ctacggtgat cctgcaaact
     9001 tcattgacca ggaacgctac ggctatgacg gcgcttacta ctccaacaac tattccagaa
     9061 ttaataatgc aacggatgaa gacctgattg caacctacaa ggagttctcc gctctggcag
     9121 aggcagcagg tgctatctat gatgaacctg acaagcgtta tgatgcatat gtagacgctg
     9181 aggtttatct cttacagcat gcgcttacga ttcctatgga gtatgagatt cagtggcagc
     9241 tgaccaagat caacgattac tccaagatga acgccatgta cggtattcag aactacacgt
     9301 ataagaactg gaagacttct gtagacgcct atactacaga acagtacaag gaattccagg
     9361 cagcatctga ggcgaaataa tatatggtaa aatataccgc caaacgactg cttcagtccc
     9421 tgattacagt gctggttgtc gccaccatcg tgttcctgct tatgcgtatg cttcccacag
     9481 attactactt tacagaggat caggtaatga agcttacgga agagcagaag aacgcgcagc
     9541 tggaggcagc cggcctgtta gatccgatgg aacagcagct gggccgtttt tatgggcagc
     9601 ttctgcggtt ggacttcggt acatcccgca gaattcagaa cggcgctcct gttcttaagg
     9661 taatcggaac taaattttcc gtatctatgc gtctggggtg tattgccctg gtgattgctc
     9721 ttttaatcgg cgtaaccatg ggtattatcc agtccagaaa taaggaccgg atgctggatc
     9781 atgtgggaac ggcgtacacg gtttttgtaa atgctgttcc gccgctggta tcctactcgc
     9841 tggtgctggc ctttggcgcc aatttgctga accttccggc gctgtattcc accagaaatg
     9901 ccacccgctc cagcatcctt ccgatcgtat gccttgcgtt aacctccatt gcccactatg
     9961 cgctgtggac cagacgatat atggtagacg agctgaataa ggattatatt aagcttgcca
    10021 ggatcaaggg tatgtcctct aagaatatta tggtaaaaca tgttctgaaa aatgcctttg
    10081 tccctctggt acagtatatt ccggcgtctt tccttttgac catcagcggt tctctcctgg
    10141 tagagcgttt cttctctgtt ccgggtatgg gtcccctgct gacagacgcc attacacgct
    10201 acgacttaaa tgtagttcag actctggtta ttatttatgc cgctctggga attatcggtg
    10261 tattcctggg cgatattctg atgatgaccc tggacccgag aattaaactg acagacaaag
    10321 gaggcagccg ttgatgaaca acgatacaca aaaggataaa ttccggtttg tggaatattc
    10381 cgcagatgcg gcagagcgaa tcggctattc cgattactcc tactggaagt ctgtttttca
    10441 gaactttttc aagaagaaat ccgccgttgt tatggcgggt gtcttctttg cactggtaat
    10501 cttttccttt attgcgctga gcattggaaa gtataactac tctgaggctc agatcatggc
    10561 aaattcgtct ctggcattta caaagccaaa cagcgaattc tggtttggaa cagataatct
    10621 gggccgtgat tactggtgtc aggtatggta tgcggctcag gtttccatta agctggctct
    10681 gatcgttgca gcaggagagt gcgtcatcgg cgtaatcatc ggctgtatct ggggctatgt
    10741 gcgcagcctg gatcgcatct ttaccgagat ttacaatgta attaacaata ttccgattat
    10801 tatttacatg actctggcag cccttgtctt tggacagagc ttccgcaata tggctcttgc
    10861 catgattgcc ttcggctggc tggtaatggc aagaaacgtg cggaacctgg tgctcatgta
    10921 ccgtgaccgg gaatataatc tggcttccag atgtcttggc actcccgtat ggagaattct
    10981 gcttaagaac attttcccat atctgatcag tgtggtaatt cttcgtctgg ccctgtccat
    11041 tccacagacc attgctcttg agtccacact gtcctatctg ggcctgggac tggatgtaaa
    11101 tacgccttct ttaggtattc ttttaagaaa tgccagaaac tatttccttc agtatccata
    11161 ccttcttgtg ttcccggcgg tgatcgtatc tcttattaca atcaccttct atctggtggg
    11221 caacgccttc tcagatgcgg cagatccaag aaatcatgta tagaacggag gaaatgttca
    11281 tggcaaatat ggaaagagaa ccgatcttat ctgccagaga cgtagagatc tccttcagcc
    11341 tgcggggcaa aaagttaaat gccatccgca gatgctccct ggatttatac cagggggaga
    11401 cgcttgccat tgtaggtgag tccggctccg gcaaatcggt atttaccaaa tccttcctgg
    11461 gaatgctgga tgccaacggt tccattaccg gcggctccat cctgtttgaa ggaaaggatc
    11521 tggcaaaata taagacagaa aaggactgga tgaccattcg gggaaagaaa atctccatgg
    11581 ttatgcagga tcctatgacc tcactgaacc ctcttaaaaa gatcggccgt cagattgagg
    11641 aaagtataga attaaatcag ggcatcaagg ggccggaagc aaagaagatg gctctggaga
    11701 tgctgaagaa agtagggatc ccggatccgg agcgccggta tcagcagtat ccccacgaat
    11761 tttccggcgg tatgagacag cgcgtggtga tcgccatagc cgctgcctgc catcctcaga
    11821 tcctgatctg tgatgagccg accacggctc tggatgttac cattcaggca cagatcttaa
    11881 atctgatccg cgatctgcaa aaggagctga acatgacggt tatctacatc acccatgatc
    11941 tgggcgtagt tgccaacgtg gcggatcgtg tggctgttat gtacgcagga cagatcgtag
    12001 agtacggtaa ggtggatgag atcttttatg atgcatggca tccgtacacc tgggcgcttc
    12061 tttccgccct gccgcagctg gggattaagg gagaagaact tcctactatc gagggaaccc
    12121 cgccgaacct attcaatgag atcaagggag acgcctttgc tcccagaaac cgtatggcgc
    12181 ttgctattga ttttgaggag gagcctccat ttttcgatgt gagtcctacc cacaaggcaa
    12241 agacctggta tctggatcca agggctccga agatcgaaca gccggattcc attcgcaaac
    12301 tccgtgaaaa aatgaaagga agagggtaag acgcaatggc agagaaagaa aaactgatag
    12361 aaatcaaaga cctgcagatt accttcggaa gcggcaaaag gaagtttgta gctgttgacc
    12421 atgtaaactt tgacatttat aagggagaga ccttttcgct ggtaggcgag tccggatctg
    12481 gaaagactac catcggacga gccattaccc ggatcaatcc tgtatccggc ggagaaatcc
    12541 tttaccgggg agagcgcatc agcggaaaaa tttcaaagga actggaccgc aaggtggtta
    12601 agtcctgcca gatgatcttc caggatccta tggcttcctt aaatgaacgg gccaaggtag
    12661 actacattgt ttcagaggga ctgatcaatt tccatcttta caaggatgaa actgacagaa
    12721 agagaaaggt agagcatgcg ctccgtgagg taggacttct gccggagttt gcttcccgct
    12781 tcccccatga attctccggc ggccagcgcc agcgtatcgg tattgccagg gcgttgatca
    12841 tggaaccgga atttgtggtg gcggatgagc cgatttccgc tctggacgta tccatccgtg
    12901 ctcaggtatt aaacctgtta aatcatttaa agcagaccag aggcctgacc tatctgttca
    12961 ttgcccatga tctttccgtg gtacggttta tttccgaccg gattgcggtt atccacaaag
    13021 gaaggatcgt ggagctggca gaggcagaag aactgttcct tcatccgttc catccttata
    13081 ccaaggcgct gctgtcggcg gtgccgatcc cggatccgga actggaaaag aaaaagaagc
    13141 ttctggttta tgatccgtcc atccatgatt acagcacaga taagcctgtc tgggaagaaa
    13201 ttctgcctgg ccattttgtc tggggcaatc agagagaact ggaaggatat cggaaagaga
    13261 tcgaaggata aaatgcaatg aataaaaaag gttcgttatt taaaacttat atgatacggc
    13321 ccgttttata caaatgcgta acaagagccg cccttgctgt aactgccctt cttctgtggg
    13381 acagatttat aaatacagcg gggcggttct cgctgtttag agacggctgt ctcacggcag
    13441 ggcttttatt tttctgtatg gcctggtttt cctatctcca tctggacggg gtaacaatcc
    13501 accatatgct ggaggagcgg aagaaaaaga agccggtgag aaaaagctat acggacattg
    13561 tggattttgc agatgagcat attgtatcgt ttgatgaatt gtcgggagag gagcgggcgg
    13621 tctgcagcat gtcggctaat ctgatctgcg gcgccctttt tatgcttccc tggctggtga
    13681 agttcctggg agatttttta tgagaaacag aaatttttag taaaaagaat tgtcgctgtt
    13741 tctgcaacat atgtccttga caaacggtca gtgaaatatt atacaaaaaa aagtgtaaaa
    13801 tcgaatgttt atacaaaagc acaggaggat gtaattatga aaaagagatt tgcggcagcc
    13861 gtatgtttaa ttgcaactgc actttctttg tcagcctgcg gcggttccgg tcagacggaa
    13921 acaacggcag caggaggagc agcagaaagc actactgcgg caccagcagc aggagtaccg
    13981 gccggcggca aggttgtaag agtcgctgca gttgacccac aggtagcgct ggatccccag
    14041 cagtatacct atagtattgt tatgaagatc acagacaatg taacagagtc tctgattact
    14101 acgatggaag acggaagtct ggagcctaat cttcttaccg ctcttccgga ggtatccgag
    14161 gacggcatga cctacagctt cgagctgctt cccgatgtga agttccacaa tggggagact
    14221 ttaaaggcct ctgatgtaaa gtattccttt gagcgtctgg taaagcttac aaagatggca
    14281 actctgatgg agaatgtaga gggctataaa gaactggctg acggtgcagc tgaggagatg
    14341 tccggtattc aggtacagga tgatactcat tttaccatca agctggttaa gccatacgcg
    14401 ccattcgctt ccgttctttc taccggatac tgtgcgattt atcctgaggc agcctgcgag
    14461 gaagcaggag atacctgggg tatgcaggta ttatacggaa ccggtccttt caagatggac
    14521 agctatcaga gcggcgtagg cgttgtactg tcaagatttg atgattacca cggcggcgct
    14581 gtgaagttag acggactgga ctacaaattt atcgaagatg taaatacaca ggtactggaa
    14641 taccagaagg gcaatgtaga ttatgtagat gtggatcctt ccatttaccc cgtatactcc
    14701 agtaatcccc agatcaagga tcagatgcac ggcttccagc ctaccggaaa ctatactctg
    14761 aacttaaatg caaagacctt cccggatcca aaggtaagac aggcaatcgc tctttctatc
    14821 gaccgcaagt cgatctgtga gagcatcctt cacggaacag caaccgttcc tacttcctac
    14881 attccggcag gcatcatcgg acatgacgag actcttcctg agttcgagta caatccggag
    14941 caggcaaagg ctcttttagc ggaagccggc tatccggacg gaattgatct gcgtgtgact
    15001 gtaaatacca agtatcaggg taacgttgca atcgttactg cattccagca gcaggcaaag
    15061 gcagccggca tccgggttga ggttgagcag gttgactccg ctgcatggtc tgatatgaag
    15121 aagaacggcg gtgttgactg cggtatttcc aactggtatg tagattactc cgatcctgag
    15181 agtatgcttt atccgatgac taagacagat tccaactcca gcttctggca caatgcagaa
    15241 tttgatcagc ttctggaaga tggaatcgca actacagatc cggccgagcg tcaggagatt
    15301 tacgcaaagg cagaacacat catgacaaga gaggactggg caaccacacc tctgtacaat
    15361 gaaacaaagt tctatctttt aaatccgaag atcactggct ttaagatgaa tgctaccttc
    15421 cgtatgttct ggaagaatgc ggatattcag cagtaatcca tgtgaaacag gggggcaaaa
    15481 ggagcgcgta atatgctccc gccccctatt ttttatatca ggaggaaatt atgctttcgt
    15541 atacggcaaa acgtctggcc cagaccattt tcgtacttct gggtatcagt cttattacct
    15601 ttatcctgct gcaggttgtt ccgggagatc cggttgccat gatgctggag aaacgtgcgg
    15661 atcccgagac tatcgccaag gttcgccacg aactgggact ggatcttcct taccatgtcc
    15721 agtaccttaa tttcttaaag ggagcggtcc gctttgattt cggaacttcc tattttacaa
    15781 aagaagtcgt ttcagacgct ttatttcgct gctttaaagt gacagtgaag ctggcctgca
    15841 tgtcttttgc atttgcagca gtgatcggta ttccctgcgg aattttctcc gcagtacaga
    15901 gaggaaaggg cattgatacg gcagtgatga tcctctccat cgtaggagtt tctgctccgg
    15961 ccttctgggt agcgatcatt cttcagatcg tgtttggctt aaagttgaac atccttccta
    16021 tttccggctt tgattctgct gcttcctata tccttcccag cgtggcttta ggcgccagat
    16081 atgcgggaag tatggccagg atcacaagaa ccagtatgct ggaggtatta aatcaggatt
    16141 acatccgtac cgccagggca aagggcgcaa agcgttggtc tgttatcctc aagcatgcat
    16201 taaagaatgc aatgatcccc attgttaccc tggtgggaac ggatttcggc tatatgctga
    16261 caggatccat gctgattgaa aaggtattct caattccggg tatcggaaag ctggcagttg
    16321 acgctatgtc aaaccgcgat cttcctcttc ttgagggaac ggttatgtat attgcgttcg
    16381 tattcgtaat cgtgaatctg ctggtagata tttcctatgc attcctggat ccaagaattc
    16441 gatatgggaa aggagcagcg taagtaagcg atgatacgtt tttttaataa acagaaacag
    16501 tttgaacagg atgtgatgga gcaggggatg cagtattcct ccggttttta cggagacagc
    16561 ctccgccgct taaagaagaa taaaatggct atgttctgtg caggaatact ggtgctttta
    16621 gtgctggttg ctatttttgc acctgtcatt gctccctatg acccaaccta tcaggactat
    16681 tctgccgtac tggcagagcc cagcatggct catccctttg gaaccgacga atacggccgt
    16741 gatattcttt ccagaattat ttacggttcc cgtgtttcat taagtatcgg tatcgtagct
    16801 caggtggttg cttctcttgt aggcgttacc ctgggcgctg ttgcaggcta ctacggcggc
    16861 cgtatcgaca gtgtgatctc ccgtattatg gaaattttcc aggcattccc ggatctgatc
    16921 tttgccatgg cgatcatgac cttcctggga aaaggtgttt taaacctgtt tattgcccta
    16981 ggacttttaa cctgggttcg tactgcccgc atgatccgcg gttctgtgat gcagttaaag
    17041 gaaaaggagt acatagaggc cagccgcgcc tgcggagcat ctaccttctg gattatcacc
    17101 aagggactgg ttcccaactg cctctctacc atcattgttt tagttaccct gggtatcccc
    17161 aatgcgatca tgtatgaggc atccttaagt ttccttggta ttggtatcca gccacctaca
    17221 ccttcatggg gaaatatgat ttctgcagct cagaccttta tcagctaccg tccggtctac
    17281 agcattgtgc ccggtgtggc aatcatgatc acggtaatcg catttaacat tttcggtgat
    17341 ggcttaagag atgcattaga tcccaagctg aaaaactgat gaaggaggac ccaatggcag
    17401 ataaaatact ggaaattaaa aatttaaaga catattttcg tactgacgcc ggactggtaa
    17461 aggcggtcaa tgacgtaacc ttttccgttg agaggggaaa aaccctgggg atcgtaggag
    17521 aatccggctg cggtaaaagt atcacttctc tttccattat gggactggtg gaaacgcctc
    17581 ccggaatcca tgcaggagga gagatcctgt ttgagggaga ggatctgtta aagaagaatg
    17641 aggatcagat gcgtcagatc cgaggcaata aggtggctat gatcttccag gaacccatga
    17701 cctccttaaa ccctgtattc accatcggcc agcagttgat ggaggccatt ctgctccatg
    17761 aaaatgttac aaagcagcag gcaagagaaa aggggatcga gatgcttaaa aaggttaaga
    17821 tccctctggc agagaagcgc tttgacgagt atccccatca gctttccggc ggtatgcgcc
    17881 agagagtgat gatcgccatg gctttatgct gcaatcccca gcttctgatc tgcgacgagc
    17941 ctactacggc tctggatgtt accatccagg ctcagatcct ggatctgatc aatgagctga
    18001 aggaagagac aggcacctct gttatgatga ttacccacga tctgggcgta attgccgagg
    18061 ttgcggatga tgtaatggta atgtatgcag gaaaggtagt agagcatgct acctgcgatc
    18121 agatctttga tgaacctctg catccctaca ccgcaggcct tatgaactgt atccccagac
    18181 tggacggcga tgacacaaag gaattaagtg tgatcgaggg catggtgcca agctttgacg
    18241 atatgcctga gggctgtgcc ttctgtccaa gatgtcctta tgcaaaggaa atctgccgaa
    18301 agcagatgcc ggagttagag gaagtaaatg gacgcagggt gcgttgcttt aaatatatga
    18361 aaggatggga ggaaagcgcc aaatgagcga acatataaat gaaacgcccc ttttggaggt
    18421 aaagcattta agaaagcttt tcccggtgaa acagacattc cggtccggag aaaagacatt
    18481 tatcaaggct gtggatgatg tttcctttac cttaaaccca aaagaaaccc ttggtgtggt
    18541 aggagagtcc ggatgtggaa aatctaccat gggacgttcc gtactgcgtc tgatcgagcc
    18601 tacagatggt gagatcatct ataaggggca agattttaaa aaggcgtccg gtcaggagct
    18661 ccggaaaatg cgtgcagaaa tgcagattat tttccaggat ccatacgcat ccttaaatcc
    18721 ccgtatgacc atcggtgaaa ttatcgagga gcccttaaag atccagaagc gtttcgcttc
    18781 aaaggaagag atgagagccc atgttttaga ggtgatggag gtcgtaggtt taaacccgaa
    18841 atattacaac cgttatcctc atgagttctc cggcggccag cgccagagaa tcggtatcgc
    18901 aagagccatt gtactagggc cgtccttagt tgtctgtgac gagccggttt ccgctctgga
    18961 cgtatccatt cagtcacagg tattaaatct tctgcgccat ctgcaggagt ccatgggaat
    19021 ggcatatatt tttatttccc ataacttaag cgttatccgc cacatttccg accgtgtgat
    19081 ggtaatgtat ctgggccatg tggtggagct ggctgataaa caccagttat atgacaatcc
    19141 ctcccatcca tatacccagg ctctgctgtc agcaattcct atgccggagc gccacagcac
    19201 cagaaagaag atcctgcttc agggcgatct tccaagtccc gcagatccgc cttccggctg
    19261 tgtgttccat acaagatgct ttatggctca ggaaatctgc tctgtgcaga agccggagct
    19321 taaagatatc ggcggcggcc atatgtgcgc ctgtcatttc tgcgggggat gccagaaata
    19381 aaatggattt aagctgtaaa tgatgtaaat taccggcccg aagcaggaag cacgggccgg
    19441 atcaaagaag gaggcaacag ttatgaagat tacggacatg aaggttcaca tgatgcaggt
    19501 tcccctggtt cgtcccttcc gcatttctct gggtgtaatt acccatgcgg tgagctgcct
    19561 ggtggagatt gagaccgacg cagggatcac cggatacggc gaaggttctc caggccctct
    19621 gatcacaggc gaaaacctgc cggggacggt agagaccctg cgggtgttcc gtgaaaagct
    19681 catcggaatg gatccaagag atattgaggg aatcaatgct gtgatgaacc gtgcggttgc
    19741 ttatgcggga actgcaaagg cggcagtgga tattgcctgc cacgatattc tgggaaaagc
    19801 agctggtctt cctctttaca agcttcttgg aggactttcc aataccattg aaacggatat
    19861 gaccgtggga attgatactc cggagcttat ggctgctgcc gcaaaggagc atgtggctgc
    19921 aggcttccgt gtgataaaga ccaaggttgg aacgaatatc gcagacgata ttgccagagt
    19981 aaaggccatc cgtgaggcag taggcgatga tgtgaaaatc cgtctggatg caaaccaggg
    20041 atggcatgca cgtgaggctg tagagctttt aaaacgcctt aacgaatacg atattgagct
    20101 ggtagagcag ccggtacgcc gctttgattt tgaaggctta aagtatgtaa ctgccaacag
    20161 ccctgtgcct gttatggcag atgagagctg ctgggactct aaggacgccc tccgtcttgt
    20221 ctctgagaga gcagtggatt tcatcaatat caagctgatg aagtgcggcg gcctgtggga
    20281 agcaaagaag atcgtaaata ttgctgaagc agcaggcgta gaaatcatgc ttggctgtat
    20341 ggcagaggag agcggaatcg ccatcaatgc agcagcggca ctgggcgcag ctctcggcaa
    20401 catcacaaga gcagacctgg atgcaagttt ctctttaaag gcgcttcctt atgagggtgg
    20461 atttaccatt gaaaatacaa aagatctggt tctgtccgag aatccaggac tgggacttgt
    20521 ccgcttaaag gaggaaatgt tccaatgagc agggcagtga cagaggaaga aatgacacag
    20581 gggatcagcc gtctggaagc ccataccgcc cagatcatgg aggaggcaaa ggccagaggc
    20641 attgccgtag gcgtggtaga ccggacggga aggatccttt atgagaaata ctttggttac
    20701 cgggatgagg aaaagaagct tcccattaac cgggatacta ttttcggaat ggcatctgtg
    20761 acaaagtctt ttactgctct ttccatcatg cagatgcaaa aagacgggat tctgtccgtg
    20821 gatgacgcag tatccgctta tattccagaa tttaccaata aaaaccagaa ggagccggtg
    20881 cgcttaagcc atctgatgag ccacagtggc ggctacttcc cccttccaag aatcgttgtg
    20941 gataaaactg ctcaggaaat ggggataaaa gacagtctgg aagaggaact ggtggacaga
    21001 gaagactttg cggaggaagg aatccgcagg gtggcaggcc gtctggacag ccagacccat
    21061 tttaccggcc gtcccggaca gcgcatgagc tactgcaatg acggcttcgg ccttctgtcc
    21121 gatattgtgc gccgccattc cggatacggt tccttcagcg catatctgaa ggagcgtatc
    21181 tttaagccac ttggcatgga gcggagcaat atcagcttcc tgcgcaattc tctggatgaa
    21241 aatgcggcca cgctttacac tctggaggat ggaacatggc gggcagacca tgattatgaa
    21301 aacgatgcct ttgtgctcca tggaggcggc gccttaaagt ccactctggg cgatatgctt
    21361 aagtatgtct ccatgtactt aaatgaaggc aaaagccagg acggaaccca ggtcattgac
    21421 cgtgattcca ttgaggaaat gtgccgtccc agacagatcg taaaaccggg cgtttactac
    21481 ggatacggcc tggaaatgag ccagatggag gacatggttt tagtagagca cggcggaagc
    21541 cttccgggcg tatcctcaaa tctgtgcttc tgtccccaga agggcatcgg agtgatcgta
    21601 ctgtgcaata ctatggatgt gccggtgtac tccatcggaa ctatggctct ccgcctgtac
    21661 tgcggcctgc ctttagaggc agagcggata gagcatatgc cacggtcctg gagtgaagat
    21721 gagaaacgtg aacttgcagg cgattacatt tccggagaag gcgaccgttt cagcatcaaa
    21781 gagctggaaa acggtgagct cgccatgaaa gtaaacggca cgcccattga gatgaagtcc
    21841 gtttatccat ggcagggaat ggtaagaaag aaatacagcg acgtatatct gaccgcgttc
    21901 agagatgaaa acggaacggt atttgccgcc agatacggaa gccgtatctt cccaaaggcc
    21961 tgacatttac aggctgaacg aaggatgtaa acttagaaag catattcaac tgaaaaagat
    22021 aaaagacaga aaaaggagct tctgttcccg gcctgaaggc tagaatgggg gctctttttg
    22081 tatgctgttc ccaaggccct ggccccgttg tcagggcttc ttgacaatgg tttctgctat
    22141 tgtataatgg gaacaggctg taaaggggca gctgtagatt tttaatgcgg agaatacata
    22201 tgaaatacca gatcacagac ggaacggttt ctctgggcgg agaaaccgtg ctttcccata
    22261 ttgattttga gatacaggga gcccagaaaa ttgcggtcgt aggacggaac ggagccggaa
    22321 aaaccaccct tctgcgcctg attgcaggag agctggaact ggagcgggat gacagacggc
    22381 aggggccggg aatctgctgt tccagaaagc tgacgatcgg gatgctggga cagcaggcgc
    22441 tgctccatga ggagcgcacc gtggaggaat acctgatgag ccgctgtccg gcagaagggc
    22501 tttttgacag ggagcggttc gaatacgaac gggaatatga ctgcctgttt acaggacttg
    22561 gttttaaaaa agaagataaa aagcggcctt taagcacatt ttcaggagga gaacggacaa
    22621 aggctgcatt ggccggactg cttcttgaga aaccggatat ccttcttttg gatgagccaa
    22681 ccaaccatct ggatatggaa acagttcagt ggctggaagg gtatttaaga aattatgatc
    22741 gggcggtggt tatggtaagc catgaccgct tctttttaga ccgtacggca cagatcgtat
    22801 acgagctgga ggacaggaag cttacaaaat atccgggaaa ttatacagcc taccgagaga
    22861 agaaacgaaa ggattacgag cttcagaaaa aggcctatat ccgccagcag gaggagattg
    22921 cccggcagga ggagctgatc cgccggttta agaataaacc ggccaaggcc tcctttgcca
    22981 gatccagaaa aaagatgtta gagcgcatgg aacgggtgga agcgcccaga gaggatacgg
    23041 cacacatttt taccggagag atccttcctg cggtgcccgg aagcaaatgg gttcttgagg
    23101 cggagcattt aaagatcggt tatgaaggcc atccgctttt ggaactgagc ctacggatga
    23161 gaaaggggca gaagattgct cttttagggg aaaatggagc gggaaagact acatttttaa
    23221 agacagccgc cggatccctt cctccggtgg atggagtctg ctcccttgga aataatatta
    23281 ccatcggtta ttttgaccag tattcctcag ccattgagtc ggaacaaacg gtagcggagc
    23341 atttttcaga gcttttcccg tccctgaacc agaaggaggt gcggacgatc ctgggctcct
    23401 atctctttcc gggaaaggag gcttccagaa aagtaagctc cctgtccggc ggggaaaagg
    23461 ccagactggt gctggcagag cttttgcaga gccgccccaa tttcctgatt ctggatgagc
    23521 ccaccaacca tatggatata caggcaagag aaactctgga atctgccttt caggcttatc
    23581 agggaaccat tctttttgta agccatgacc ggtattttat ccggcaggtg gcaaagtcgg
    23641 tgcttatatt tgagggtgcg agggctatgt attacccctt tgggtacgag cactatctgg
    23701 aacggctgga aaaggaaaaa gaaggacttt cgccggcagc acggattcag gcggaggagc
    23761 aggctctgat cgccggctta aaggcagttc ccagggcgga acgccacagg ctgagagaga
    23821 ttcccaccga agaagcctat gaccagtgga ggcttaaaat ggcagaggat gccatgaatg
    23881 aggcggcagg acaggcagag gagcagatga aggtatggga ggagaaccgt ctgaaacgtc
    23941 tgtgggagca ggccgaggag cagccagctt ccgctttgga agcccagccg ttggaggata
    24001 tagcgcctct gtgggagaag tggcatcagg cctgcatgga ctggtatgag atatggagtg
    24061 agctgcaccc ggagccggag gcgcctgaac cggaaaagtt cgctgatttg gaatcaaagg
    24121 aggatgcaga atgatactga taaaaaatgc agacgtatat gcaccggagc atctggggat
    24181 taaggatgtg ctgatatgcg gagcacagat tgaggcagtg ggagaaggtc tggaaggagg
    24241 aagcggctgc cggatcatag atgcagaggg aaaaaagctg acgccgggac tgattgaccg
    24301 ccatgtccat gtgaccggag gaggcggaga aggaagtttt catacccgta cgccccagat
    24361 acagctttca tctgttataa gagctgggat cacgactctg gtgggccttc tgggaacaga
    24421 tgatgtaagc cgtacgccgg aggatctgat cgccaaggta aaggccttaa aggaggaagg
    24481 aatttcagcg tatgccctct gcggcgctta tggtgtgcct tcccctgcca ttacaggaag
    24541 cataaagaag gatattgcct ttattgatga gattttgggt ttaaagctgg cagtctccga
    24601 ccacagagca cccaatattt ccacagagga gctgatccgt ctggcaagcg atgtgcggac
    24661 tgcaggcatg atcagcggaa agtgcggctg tgtggtgctt catatggggg atggaaaaaa
    24721 gatgctgagt caggtttttg aggcactgga ggagacggag attcccgtga aaacctttca
    24781 gcccacccat gtaaaccgca atcatgagct gtttctggac agccttaagc tggcgaagct
    24841 tgggggaacc attgatctga cctgtgagga tttccctcat ggtttgatcg aagacggaaa
    24901 ggatcctgtg atcggccttt tggaggaagc ccgccgg
//
LOCUS       DBMDUH010000021        24399 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_1378_length_24399_cov_5.436250, whole genome shotgun sequence.
ACCESSION   DBMDUH010000021 DBMDUH010000000
VERSION     DBMDUH010000021.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 24399)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 24399)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24399
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1378_length_24399_cov_5.436250"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /gene="yqeC"
                     /locus_tag="ACZPCG_03325"
     CDS             <1..426
                     /gene="yqeC"
                     /locus_tag="ACZPCG_03325"
                     /inference="COORDINATES: protein motif:HMM:TIGR03172.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HIR1284137.1"
                     /translation="VLVEADGAKRLPVKVPGPAEPVIPGDTDLILCVYGLKALGQKGE
                     KCCFRMEQAKTLLGESFSLDKEDREWAMEEKYMACLMKQGYLSPLKQSFPQARILPVF
                     NQADTGELVLAGKRILGQIGHTEGLITGNLWKEPSFELF"
     gene            453..1076
                     /locus_tag="ACZPCG_03330"
     CDS             453..1076
                     /locus_tag="ACZPCG_03330"
                     /inference="COORDINATES: protein motif:HMM:NF024214.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR1284138.1"
                     /translation="MKLSMIYMASGFGKRFGSNKLLASFEGRPLYTYGLSALEKGAQI
                     LEKKSGISCEIITVSQYPQILESARCLISGEKVLENPFSEEGITASIRLGVAAAWEDT
                     DAFLFFVADQPYTEGEMIASFAEGYFKSGKKLGCTAFKGRRGSPTAFGACYRSELLAI
                     SGDKGGRQVMKNHPEELWIMETEEKQLWDVDLPEDLKRGKKEYGTGA"
     gene            1060..2436
                     /locus_tag="ACZPCG_03335"
     CDS             1060..2436
                     /locus_tag="ACZPCG_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004462067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1284139.1"
                     /translation="MAQEHDFSKGSIISNILKLALPMTLAQLINVLYNIIDRIYIGQL
                     PEDATLSLTGLGLCLPIISIVIAFANLFGMGGAPLCSIERGRGNKEEAEAIMGNSFVM
                     MVLVGLLLTTAGLLFKRPLLYLFGASDATIGYADDYVTIYLLGNIFVMVGLGMNSFIN
                     SQGFGTIGMMTVLLGAAANILLDPVFIFGFHMGVQGAALATILSQFLSAVWILRFLTG
                     PKATLRLRRSSMRLERERIFRIMGLGMSGFTMAITNSSVQIMYNTMLQRVGGDLYVGI
                     MTVINSVREVISMPAQGVTNSAQPIMGYNYGAKEYGRVKKAIVFMSSVSVLYTLAAWG
                     LIHGFSEFFIRMFNRSGELVEAGIPAMRIYFFGFFMMSLQFAGQSVFVGLGKSRYAVF
                     FSIFRKVIIVIPLILILPTIFHLGTDGILMAEPISNFIGGTACFGIMLCTVWRELGKK
                     EKSNAQET"
     gene            2420..4060
                     /locus_tag="ACZPCG_03340"
     CDS             2420..4060
                     /locus_tag="ACZPCG_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007713114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HIR1284140.1"
                     /translation="MHRKHKSRTWVAALFVGTAILFTCGFGRMSEYSSGERGYLRSAT
                     YYSDDWVINFWNSESVHMEEELARIAADGFNSIILAVPWREFQPETAPVMYEEYAWKK
                     LGGVMDAAGKQGLGVILRVGYTWDYASPESALSRYEGLLYDPQLKAAWISYAERLYKQ
                     VSAYENFCGGFLTWEDFWNFTENAAGYGNGIAGRRMAKLCGYTDYLKEKYTLEQLKEY
                     YGTSFENYEEIWLPEKDSYARKLFYEFYDAFLAELLTETQKVFPDLSMEVRLDADPVK
                     KADKTLEGVSHQSTFGCGNASCTSIMYSVPMGFPNEGEWVTADQALNMAPVFFDQVSR
                     YNGNKPVYVDQFLFTDNTPGFERNARLKPEEKNAYLAGMGELFRTMTIGYGVWTYRDY
                     GDNKLYNPQFALGLRGWKVTGESYIEEGKNGKEAVLPSGSSISQEMRGRVTGRTGPDV
                     HVRFSLEGTAGSTVSVRVGKRTKTFQAGERTDAELVFENALAEELTISCTGGGSVRID
                     DLNLYTFVTEGDIYRMDGSEGECTDGIRQLNQALGAED"
     gene            4165..4479
                     /locus_tag="ACZPCG_03345"
     CDS             4165..4479
                     /locus_tag="ACZPCG_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HIR1284141.1"
                     /translation="MTKLECSVENCVHNADQCCCRGSILVDGHGARDAEGTCCASFEE
                     NKGGSFKNLFKTPETRLEVDCDAVNCVYNEDCCCTASGICICGNGACDCENTRCATYK
                     AK"
     gene            4789..5283
                     /gene="infC"
                     /locus_tag="ACZPCG_03350"
     CDS             4789..5283
                     /gene="infC"
                     /locus_tag="ACZPCG_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008722378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HIR1284142.1"
                     /translation="MINEQIRDREIRLIGENGEQLGIMSARDAYKMAQEAELDLVKIA
                     PTAKPPVCKIIDYGKYRYELARKEKEARKKQKVIDVKEVRLSPNIDTNDLNTKVGAAR
                     KFLEKGDKVKVTLRFRGREMAHMSKSKYILDDFAESLADIAVVDKPSKVEGRSMVMFL
                     TAKR"
     gene            5315..5512
                     /gene="rpmI"
                     /locus_tag="ACZPCG_03355"
     CDS             5315..5512
                     /gene="rpmI"
                     /locus_tag="ACZPCG_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HIR1284143.1"
                     /translation="MPKLKTSRAAAKRFKKTGTGKLVRNKAYKSHILTKKSTKRKRNL
                     RKDIVTDATNVKAMKKILPYL"
     gene            5542..5898
                     /gene="rplT"
                     /locus_tag="ACZPCG_03360"
     CDS             5542..5898
                     /gene="rplT"
                     /locus_tag="ACZPCG_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HIR1284144.1"
                     /translation="MARIKGGLNAKKKHNRILKMAKGYRGARSKQYRVAKQSVMRALT
                     SSYAGRKERKRQFRQLWIARINAAARINGLSYSKFMYGLKLAEVDLNRKVLSDMAIND
                     AEGFAKLAELAKSKLA"
     gene            6057..7406
                     /locus_tag="ACZPCG_03365"
     CDS             6057..7406
                     /locus_tag="ACZPCG_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1284145.1"
                     /translation="MAQNVKDSTDMIHGSIARSILLFSIPLLVGNFFQQLYNTVDAYV
                     VGNYVNTSALAAVGASTPIVNMLVGFFTGLAAGAGVVISQYFGGGRTEQMRRAIHSSL
                     ALTAILSVLFTALGLSFTKPLLHAIGVPEDVMPNSALYLSIYFWGMTFSLFYNMGSGI
                     LRAIGDSKNPLFYLIAASLVNIVLDFLFVCGFGMGVEGAAIATIIAQAVSAALVMGKL
                     LMTREIYRVRLGEICFDRKMIRRIVDIGLPAAIQQSITSFSNVIVQSYINYFGTAAMA
                     GYSATLRVDGFLQLPLQSFNMTITTFVGQNIGAKEYKRVKKGIFTSWLMCSGIILVGS
                     VIMFLKAPSIIGIFTEDAAVISVGSSMIRIFGSAYLFLPIIQILNGALRGAGRSKVPM
                     YFMLGCFVLLRQIYLMIAVPMTKNVSVVFAGWPVTWVVCAIGMLVYYFKVDWLPKEE"
     gene            7501..7589
                     /locus_tag="ACZPCG_03370"
     tRNA            7501..7589
                     /locus_tag="ACZPCG_03370"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7535..7537,aa:Ser,seq:cga)
     gene            complement(7673..8560)
                     /gene="rsiV"
                     /locus_tag="ACZPCG_03375"
     CDS             complement(7673..8560)
                     /gene="rsiV"
                     /locus_tag="ACZPCG_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-V factor RsiV"
                     /protein_id="HIR1284146.1"
                     /translation="MEQKDLKLSGYRQLEQLRQEYESLPVPLEARDRILAGIAQGKKE
                     TDKAQMKGVIFMKLMKRTGMTAAAAMVAITLLTNLHPTIANAMEQIPVIGPIAKVVTF
                     RTYENKTNNFEANIQIPQIEAAPETLNKSIEEYAQELIAAYEGELSQSQGEGSYSLDS
                     SYKVVTDNDRYLCLRIDTTIVMASGAQSTKIFTIDKTTGKQLSLKELFKDRPEMLSSI
                     TANIKEQMASQMAADDSVSYFYNSDMPEDDFKELTGDESFYFNEEGLLVIAFDEYEVA
                     PGYMGAVEFTIPSSVAGTF"
     gene            complement(8574..9074)
                     /locus_tag="ACZPCG_03380"
     CDS             complement(8574..9074)
                     /locus_tag="ACZPCG_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR1284147.1"
                     /translation="MDTNPITKQTEQILIDGYERFYRLAYSYVQNREDALDVVQESAC
                     KAIRDCGQVKNTDFLSTWIYRIVINTSLDLLRKNKKEIPAEEMPEIPWEDKYQELDLK
                     TALKHLDEKSRTIVMLRYFEDMKLEDISRVVDENLNTVKARLYRALKKLRITMEPEEP
                     KKSYAK"
     gene            9346..10926
                     /locus_tag="ACZPCG_03385"
     CDS             9346..10926
                     /locus_tag="ACZPCG_03385"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006428043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HIR1284148.1"
                     /translation="MIEWKNFDSLESYGELAKAERVKLAEVMSGESGAERVKAYSVPM
                     AAGMVYNYAAKQVDENVLAVLKKLAKEAQLSEKYKALYNGEMINTGEKRLVLHQLTRG
                     QLGDKVVADGTDKRAFYVEQQERIAEFAKKVHSGEITNAAGEKFTTVVQIGIGGSDLG
                     PRAMYLALENWAKKHNTFKMEAKFISNVDPDDAAAVLGSIDVAHSMFVLVSKSGTTLE
                     TLTNESFVKDALKKAGLDASKHMVAVTSETSPLAKSDDYLAAFFMDDYIGGRFSSTSA
                     VGGAVLSLAFGPEVFAQFLEGAAEEDKLAAKEDLLQNPDMLDALIGVYERNMLGYPAT
                     AVLPYSQALSRFPAHLQQADMESNGKSVNRFGEPVNYVTGPVLFGEPGTNGQHSFYQL
                     LHQGTDIVPIQFVGFKNSQIGTDVVIEDSTSQQKLCANVAAQIVAFACGKDDENLNKK
                     FEGGRPSSIIIGEQLNPKALGALLAHFENKIMFQGFVWNVNSFDQEGVQLGKVLAKRV
                     LAHETDGALQVYSELLNI"
     gene            11064..11816
                     /locus_tag="ACZPCG_03390"
     CDS             11064..11816
                     /locus_tag="ACZPCG_03390"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007709856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0016019 - peptidoglycan immune receptor
                     activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045087 - innate immune response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein family
                     protein"
                     /protein_id="HIR1284149.1"
                     /translation="MDEDKRLEEELKELRQVPIDENDRKKKKSRRSRKKTAKRRGFPF
                     AAVFMALVVGVLAGLGGGYYVWGWERPYTVDLKAVEVPDWVKQEFIRKNIFSRPDVSR
                     RKINNIVLHYVANPGSSAEGNRNYFDSLADQDSQKGGTSTSSHFIVGLEGEVIQCIPT
                     NEIAYANAPRNEDTLSIEVCHPDDTGKYSDATYESVVRLTAWLCTQLKLKSSDVVRHY
                     DINGKLCPKYYVEHEEEWKQLRKDIDAAMKEL"
     gene            complement(11850..12659)
                     /locus_tag="ACZPCG_03395"
     CDS             complement(11850..12659)
                     /locus_tag="ACZPCG_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002571218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HIR1284150.1"
                     /translation="MICDCHLHTAFSGDSNTPARVQIERAVSMRMKEITITDHHDRDS
                     SFCEDNFELDLPSYLSALNTLKEEYRDKIRINIGIELGLQLHICRYLEKFHKDFGSKF
                     DFIIGSSHFIHSHDPFYPSFWEACGSRQGLEEFFTLSFKRARAFHPFFDSWGHLDYAA
                     RYAPDSASVYSYFAFKEQIDSILRVLAENGKALECNTGGFRSRLSHQNPDTEILKQYR
                     RMGGEHITIGSDAHAPEHLGYGFTRAKELLKQCGFHYYTIYHNRKAEMIPL"
     gene            12927..13958
                     /gene="gap"
                     /locus_tag="ACZPCG_03400"
     CDS             12927..13958
                     /gene="gap"
                     /locus_tag="ACZPCG_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HIR1284151.1"
                     /translation="MAVKVAINGFGRIGRLAFRQMFGAEGYEVVAINDLTDPKMLAHL
                     LKYDTAQGGYAGHIGEGKHTVEAGEDSITVDGKKITIYKEPKAADLPWGEIGVDVVLE
                     CTGFYCSKDKAQAHIDAGAKKVVISAPAGNDLKTIVFSVNEDTLTAEDTIISAASCTT
                     NCLAPMAKALNDYAPIQSGIMSTIHAYTGDQMILDGPHRKGDLRRARAGAANIVPNST
                     GAAKAIGLVIPELNGKLIGSAQRVPVPTGSTTILTAVVKKAGVTKEDINAAMKAAASE
                     SFGYNEDPIVSSDVIGMKYGSLFDATQTMVAQIAEDLYEVQVVSWYDNENSYTSQMVR
                     TIKYFAELA"
     gene            14043..15266
                     /locus_tag="ACZPCG_03405"
     CDS             14043..15266
                     /locus_tag="ACZPCG_03405"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HIR1284152.1"
                     /translation="MMLNKKSIDDMNVKGRRVLVRCDFNVPLKNGEITDETRIVAALP
                     TINKLIADGAKVILCSHLGKVKNGPNEGESLAPVAKRLSEKLGKEVVFVADYNVTGEA
                     ATKAVEAMNEGDVVLLQNTRFRGAEETKNGEAFSKELADLADDYVCDAFGSSHRAHAS
                     VEGVTKFISAKGGTNAVGYLMQKEIDFLGNAVENPVRPFVAILGGAKVADKLNVISNL
                     LEKCDTLIIGGGMAFTFLKAEGKEIGKSLVDDTKLDYCREMMAKAEKLGKKLLLPVDA
                     VVAASFPDPIDAPVEAENVSVDAMPADKMGLDIGTETAKIYAEAVKSAKTVVWNGPMG
                     VFENPTLAKGTIAVAESLAETDATTIIGGGDSAAAVNQLGFADKMSHISTGGGASLEF
                     LEGKVLPGVAAADDK"
     gene            15362..16117
                     /gene="tpiA"
                     /locus_tag="ACZPCG_03410"
     CDS             15362..16117
                     /gene="tpiA"
                     /locus_tag="ACZPCG_03410"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HIR1284153.1"
                     /translation="MARKKIIAGNWKMNMTPSEAVALVNELKPLVANDEVDVVFCVPA
                     IDIIPAMEAAKGSNINIGAENMYFEEKGAYTGEISPNMLTDAGVKYVIIGHSERREYF
                     AETDETVNKKVLKAFEHGITPIICCGESLTQREQGITIDWIRQQIKIAFLNVTADQAK
                     TAVIAYEPIWAIGTGKVATTEQAEEVCAAIRACIGEIYDEATAEAIRIQYGGSVSAAS
                     APELFAQANIDGGLVGGASLKPDFGKIVNYKQA"
     gene            16140..17681
                     /gene="gpmI"
                     /locus_tag="ACZPCG_03415"
     CDS             16140..17681
                     /gene="gpmI"
                     /locus_tag="ACZPCG_03415"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HIR1284154.1"
                     /translation="MNKKPVVLMILDGYGLNKNCDHNAVCEAKTPVMDQLMSQCPFVE
                     GQASGLFVGLPDGQMGNSEVGHLNMGAGRVVYQELTRITKSIEDGDFFQVPEFLQAVE
                     NCKKNDSALHLFGLVSTGGVHSHLTHIYGLLELAKRNGLEKVYVHCFLDGRDTPPESG
                     KDFVAQLEEKMAEIGVGKVASVMGRYYAMDRDKRWDRVELAYRALTKGEGKAAESATA
                     GIQASYDDGKADEFVVPFVVTENGAPRASIKDGDSVVFFNFRPDRAREITHAFCDDEF
                     DGFAREKKLDLVYVCFTDYDETITNKLVAFKKEQITNTFGEFLAAHGKKQVRIAETEK
                     YAHVTFFFNGGVEEPNEGEDRILVPSPKEVATYDLKPEMSANGVCDKLTEAIRSGKYD
                     VVIINFANPDMVGHTGVENAAIKAIETVDACVGKAVEAVKEVDGVMFICADHGNAEQL
                     VDYETGEPFTAHTTNPVPFILVNADPSFKLREGGALCDIAPTLIELMGLEQPKEMTGK
                     SLLVK"
     gene            complement(17787..18671)
                     /locus_tag="ACZPCG_03420"
     CDS             complement(17787..18671)
                     /locus_tag="ACZPCG_03420"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR1284155.1"
                     /translation="MGYVYLLCVALMFSFGGTCVKLISPYFNASWITFFRFFIGVVWL
                     LILKCILRQPFQKNLKQALLSCWKWILFGAFIKWMAYLTENYALSHGLSYGNIVTQPA
                     QTLFLTLVSVLCFKEKLSPRKGFCIFLCIGGVLCISLNGRPLDVFFKENLLFTGLFIL
                     SGICAGCHVLSQKMVAGRLDIIDSNLAIFTLSGLFSLIPAAEPVFTGKIAFIRPDLAC
                     ILAILAFGFITGMGFYLNAKAIPLVPFYMVPVIQSMMAVFAILWGVFFFHETVTGWII
                     GGTAAFMIGVIGLQMKNN"
     gene            18848..20623
                     /locus_tag="ACZPCG_03425"
     CDS             18848..20623
                     /locus_tag="ACZPCG_03425"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008722680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1284156.1"
                     /translation="MNTKKGMKAAKLLKSFLPYYGKYKKVLILDLFCAALTTLGELVL
                     PLILRFITNQGMQDLASLTTGLVVKIGLLYLILRVIDSLAAYYMAYTGHVMGVYIETD
                     MRQDAFEHLQMLSDSYYSNTKVGQIMSRITSDLFDVTEFSHHCPEEFFIAILKGIVSF
                     VILSGINLELTLLIFLMIPIMIVSCTWFNLKVKAAFRLQRNQIGELNAQIEDTLLGNR
                     VVRAFANEPVEIEKFGKGNREFMKIKKHTYRYMAAFQITTRSFDGLMYVLVIVAGGLF
                     MINGKIAPGDLVAYTMYVTTLLTTIRRIIEFAEQFQRGMTGIERFQEIMDVTPDIRDK
                     KGAKPLSDVKGTIEFDHVSFEYADDHTQVLNDICLKIRPGERIALVGPSGGGKTTLCN
                     LLPRFYDTTGGTISIDGMDVRDITLRSLRSNIGVVQQDVYLFSGTVFENILYGRPGAS
                     REDVIEAAKMAGAHEFITELKDGYDTYVGERGVKLSGGQKQRISIARVFLKNPAILIL
                     DEATSALDNESEYLVSQSLEKLAEGRTTLTIAHRLTTIQGADRILVLSGNEIVEEGNH
                     KELLEKKGMYYQLYMTANRLNQGLN"
     gene            20638..21507
                     /locus_tag="ACZPCG_03430"
     CDS             20638..21507
                     /locus_tag="ACZPCG_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIR1284157.1"
                     /translation="MKIAAATDSNSGITQDQAKQLGVHVLPMPFIIDGQMYYEGVDLT
                     HEEFFRRMEEGADITTSQPSPGEVTDFWDKLLEEYDAVVYIPMSSGLSGSCQTAMLLA
                     EDYEGKVYVVNNQRISVTQMQSVLDARDLIKKGYAAAQIRNILEETKFDSSIYVTVTT
                     LKYLKKGGRITPAAAMLGTLLQIKPVLTIQGEKLDAFSKARTLKQGKTIMMTALKKDL
                     ETRFHDPEARHTWLEIAYTCSDEMAQEFKETVAEIYPDANICIAPLSLSIACHIGPGC
                     LAVACSGHLKELE"
     gene            21560..22978
                     /locus_tag="ACZPCG_03435"
     CDS             21560..22978
                     /locus_tag="ACZPCG_03435"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007709959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1284158.1"
                     /translation="MIPMVILMILTALVPMLLQAMISRRAFNQTQLEAKGIEIQNQCL
                     ILSSRLMRSGYMTSDKGNTMELDTRMQTIADIYNGRIVIVNSSFKIMKDTFNLAAGKY
                     YVSEEVIRCFNGETNSRMNQEMGYLTQTIPIHSASDKKKVTGVMVVMASTENISALTD
                     RVQGSTQLFLLCMILVVVVCSVAAVFFLIRPFKRLLASFERVSYGDLDTDIMEDAYKE
                     TIQLSQAVQKSLRKLKAVDQSRQEFVSNVSHELKTPITSIRVLADSLMSMEEVPAELY
                     REFMADISDEVDRENQIITDLLTLVKMDKSAQSQMNITQTNINEELELILKRLRPIAK
                     RSNVELILESIREVTGDVDRVKFSLAVSNLVENAIKYNVDSGWVRVTLDADHKYFYIR
                     VADSGIGIPQDCMDHIFERFFRVDKARSREVGGTGLGLAITLNVIQMHHGIIDAESTP
                     GEGTTFSVRIPLNYVSRQEDKS"
     gene            22975..23931
                     /locus_tag="ACZPCG_03440"
     CDS             22975..23931
                     /locus_tag="ACZPCG_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HIR1284159.1"
                     /translation="MSTTGKKGRFSVKILLRLFCLTGILFLSGCHLEKEPVAAPKEGR
                     YLVYYLNGSGVNLIPRVYETEETDPLALSDELMEQLRYVPKDLDCQTALGDKAVFRES
                     RLDAGVLSLYFDVSYVNMKPEQEILCRAALARTLTQVPGVDYISIYCGDQPLMDGQGN
                     PVGAVSASDFILNTSNVNSYEKTELKLYFADKTGTKLISETREVVHNINTSMEQLIVE
                     QLLAGPAEEGHLAVLPSDCKILSISVTDNVCYINFDSSFLKMEHPVNEYLPIYAIVSS
                     LAEMTSITRVQIMVNGSQDVMFRDVVSLNQTFELNHEYIQEE"
     gene            23941..>24399
                     /locus_tag="ACZPCG_03445"
     CDS             23941..>24399
                     /locus_tag="ACZPCG_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284160.1"
                     /translation="MNMKRPIAGIAAGFVLGEVLVLQMQEAVNVWLTVVLLIPALFCV
                     VLKWGTAKERRTGRSGIKKKLLSVFLISLAMASGSGRGGMVKNRLDHEEGLVKPFEGK
                     QCRIRGTVEKRSWKEERLTLELKESELQSGERKETFRRILLYMDYGNVPDW"
BASE COUNT         6682 a         4987 c         6543 g         6187 t
ORIGIN      
        1 gtcttagtgg aggccgatgg agcaaagagg cttccggtga aggtgccggg accggcagag
       61 ccggtgatac cgggagatac agatctgatc ctctgcgtgt acggtcttaa ggctctggga
      121 caaaagggag agaaatgctg ctttcgtatg gaacaggcaa aaacgctttt gggagagagc
      181 ttcagtctgg ataaagagga cagggaatgg gcaatggagg agaaatatat ggcctgtctg
      241 atgaaacagg gatatttaag tccgctgaag cagtcatttc ctcaggccag gatccttcct
      301 gtgttcaatc aggcagatac aggggagctt gttctggcag gaaagcggat cctggggcag
      361 atcggacata cagagggtct gatcacggga aacctgtgga aggaaccgtc atttgagttg
      421 ttttaaacag gatagaaagg tgactttata caatgaaact ttcgatgatt tacatggctt
      481 ccggctttgg aaagcgattt ggttccaata agcttctggc atcctttgag ggacgtcctc
      541 tttatacata cggattgtct gctctggaaa aaggggcgca gatcctggaa aaaaaatcgg
      601 ggataagctg tgagataatt acggtaagtc aatatcccca gatcctggaa agtgcccgct
      661 gcctgatctc cggagaaaag gtgctggaaa atccgttcag tgaagagggg ataacggcgt
      721 cgatccgcct tggggtagcc gccgcctggg aagatacgga tgcattcctt ttttttgtgg
      781 cagatcagcc ctatacggaa ggagaaatga tcgcttcttt tgcggagggc tattttaaaa
      841 gcggaaaaaa gctcggctgt acggcattta agggacgcag gggaagtcct actgcgtttg
      901 gagcctgtta ccgctctgag cttttggcta tttcaggaga taagggtggc cgtcaggtta
      961 tgaagaacca tccggaggaa ctctggatca tggaaacaga ggaaaaacag ctgtgggatg
     1021 tggatctgcc ggaggatttg aagaggggga aaaaagagta tggcacagga gcatgatttt
     1081 tcaaagggca gtatcatttc aaatatttta aagctggctc ttcctatgac gctggcccag
     1141 ctgatcaatg tactctataa tatcatagac cggatctata tcggccagct tcctgaggat
     1201 gctacgctgt ctctcacagg tcttgggctg tgtcttccta ttatttccat cgtaatagcc
     1261 tttgcaaatc tctttggaat gggaggtgca cccttatgct ccattgagcg gggaagagga
     1321 aataaggaag aggcagaggc cattatggga aattcctttg taatgatggt actggtgggg
     1381 ctgctcctta ccactgcggg ccttctgttt aagcggccgc ttttatatct gtttggagcc
     1441 agcgacgcta ccattggcta tgcggatgac tatgtgacga tttatcttct gggaaatata
     1501 tttgtgatgg tcggactggg tatgaacagc tttatcaatt ctcagggctt tggaaccatt
     1561 ggaatgatga cggttctttt gggagcagcc gccaatattt tactggatcc tgtatttata
     1621 tttggatttc atatgggggt tcagggagcg gctctggcta ccattctttc ccagtttctt
     1681 tcagctgtct ggatcctccg gttcctcacc gggcccaagg ccactctacg gctgcgccgt
     1741 tcatccatgc ggctggaacg ggagcggatt ttccgtatca tgggtctggg aatgagcggg
     1801 tttaccatgg cgatcaccaa cagttcggtg cagatcatgt acaacaccat gctgcagagg
     1861 gtcggaggtg atctttatgt ggggattatg accgtcatca attctgtccg tgaggtgatt
     1921 tccatgccgg ctcagggcgt gaccaacagc gctcagccga tcatggggta taattatggg
     1981 gcaaaggagt atggacgggt aaaaaaggcc attgtattta tgtcttctgt ttctgtttta
     2041 tatacattgg ctgcctgggg gctgatccat ggtttttccg aattttttat ccggatgttt
     2101 aaccgcagcg gagaactggt ggaggcggga ataccggcta tgcggattta ctttttcggc
     2161 ttttttatga tgtccctgca gtttgcaggc cagtccgtat ttgtaggtct gggaaaatct
     2221 agatatgccg tattcttttc cattttcagg aaagtgataa tcgtaatccc gctgatattg
     2281 atcttgccga caatttttca tctgggcaca gacggtatcc ttatggcaga gcccatatcc
     2341 aattttatcg gcggaacggc ctgtttcggg atcatgctgt gtacggtatg gcgcgaactg
     2401 ggaaagaagg agaaatcaaa tgcacaggaa acataagagc aggacatggg tggcggccct
     2461 gtttgtggga accgccattc tttttacatg cggatttggc agaatgtcgg agtattcttc
     2521 tggagaaagg ggctatctgc gttctgcaac ctattattcc gatgactggg tgatcaattt
     2581 ctggaacagc gaaagtgttc atatggagga ggaactggca cgtattgcgg cagacggctt
     2641 taacagtatt atcctggcag ttccatggag ggaatttcag ccggaaacag ctccggtcat
     2701 gtatgaggag tatgcctgga aaaaactggg gggcgttatg gacgcagccg gaaaacaggg
     2761 attgggagtg atcctcagag tgggttatac ctgggattat gcgtcaccgg agtctgcttt
     2821 gtcacggtat gaaggacttt tatatgatcc gcagctaaaa gccgcatgga tttcctatgc
     2881 agaacgtttg tataaacagg tttctgctta tgaaaacttc tgcggcggct ttctcacctg
     2941 ggaagatttc tggaatttca cggaaaatgc agccggatat ggaaatggga ttgccggacg
     3001 cagaatggca aagctgtgcg gatatacgga ttacctgaag gaaaaatata cgctggagca
     3061 gctaaaggaa tattatggaa cttcctttga aaattatgag gaaatatggc ttccggagaa
     3121 ggactcttat gcaagaaaac ttttttatga attttatgat gcgtttcttg cagagctttt
     3181 gacggaaact cagaaggtat ttccagatct ttccatggaa gttcggctgg acgcagatcc
     3241 ggtaaaaaaa gcggacaaaa cgctggaagg tgtttctcat cagagtacct ttggctgtgg
     3301 aaatgcatcc tgcaccagta ttatgtacag tgtccctatg gggtttccta atgaaggcga
     3361 gtgggtgaca gctgatcagg cattaaacat ggcgcctgta ttttttgatc aggtcagccg
     3421 gtataatgga aataaaccgg tgtatgtgga ccagttcctt tttacggaca atactcctgg
     3481 ttttgaaagg aatgcccgtt taaagccgga ggagaaaaac gcctatctgg ccggaatggg
     3541 tgagctgttt cgcaccatga ccataggata cggcgtgtgg acttacaggg attacggaga
     3601 taacaagctt tataaccccc agtttgccct gggactgaga ggctggaagg taaccggaga
     3661 aagctatatc gaagagggga agaatggaaa agaagcggtt cttccttcag gaagcagcat
     3721 ttcccaggag atgcggggca gagtcaccgg cagaactggc ccggatgtcc atgtgcgttt
     3781 ttctctggaa ggaacagccg ggagtacggt aagcgtccgg gtggggaagc ggactaagac
     3841 ctttcaggca ggagaaagaa cggatgcaga attggtattt gagaatgcat tggcagaaga
     3901 actgaccatt tcctgcaccg gaggaggcag cgtacggatt gacgatctga atctatatac
     3961 gtttgtgaca gagggtgata tataccgcat ggatggctcc gagggggaat gtacggatgg
     4021 gatccggcag ctgaaccagg ctctgggggc agaggattaa ggtatttttg catgagaaaa
     4081 gccaccagag cgcatactaa atcctgtcat gacgcctgaa accttacgtt ttgggaaaat
     4141 ttatgtgaca ggaggaaatc aaatatgaca aaattggaat gttcagtgga aaactgtgtg
     4201 cacaatgcag atcagtgctg ctgcagaggc tcgatcctgg tagacggaca cggagccagg
     4261 gatgcagagg gtacctgttg tgccagcttt gaggaaaata agggcgggtc ttttaaaaat
     4321 ctctttaaaa ccccggaaac ccgtctggag gtagactgtg atgccgtaaa ctgcgtctac
     4381 aatgaggact gctgctgtac ggcgtccgga atctgtatct gcggaaacgg agcctgcgac
     4441 tgtgaaaata caagatgcgc cacttataag gcaaaatagg aaacttaaaa aagtagttga
     4501 cattcccgga atcatatggt atcattttgg aagttgtgaa aacaacgcac aggaaagcag
     4561 gttatccgcc tcaccttggc acgagtcagt gacccgggtt tatatcagaa gctgtataaa
     4621 aggtttttta tataaagaat ctttttcggt ttaaaaccag agcagcagac gagtatgatt
     4681 ttttagcgga tggcataagc tgtccgcttt ttacatttac caagcagagg caaaggctct
     4741 tcgttcaaac aaacatgact atggaggtgt acgactatta gcgagttaat gatcaacgaa
     4801 caaatcagag acagagagat ccgtctgatc ggtgagaacg gcgaacagtt aggtattatg
     4861 tctgcaagag acgcctacaa gatggcgcag gaggcagagc tggatcttgt gaagatcgct
     4921 cctactgcaa agccgccggt atgcaagatc atcgattatg gaaagtaccg ttacgaattg
     4981 gcccgtaagg aaaaggaagc cagaaagaaa cagaaagtga ttgacgtcaa agaagttcgt
     5041 ttgtccccga acattgatac taatgactta aacaccaagg tgggagctgc cagaaagttc
     5101 ctggaaaagg gtgataaggt aaaggtaact cttcgtttcc gtggccgtga gatggcccat
     5161 atgtccaagt caaagtacat tctggacgat ttcgctgaga gtctggctga catcgcagtt
     5221 gttgataagc cctcaaaggt ggaaggaaga agcatggtta tgtttttaac tgcaaaacgt
     5281 taaaaataga aagttttcaa ggaggaaatt tacaatgcca aagttaaaaa ccagcagagc
     5341 agcagcaaag cgctttaaaa agacaggaac cggtaagtta gtccgcaata aagcttacaa
     5401 gtctcatatc ttaactaaga agtctacaaa gagaaagaga aatcttagaa aagatatcgt
     5461 taccgatgct actaacgtaa aggcaatgaa gaagatttta ccatatctgt aagttttaga
     5521 ttaaattagg aggaattacc gatggcaaga attaaaggcg gcttaaacgc aaaaaagaag
     5581 cacaatagaa tcctgaagat ggctaagggc tacagaggag cccgttccaa gcagtacaga
     5641 gtagctaagc agtccgtaat gcgcgcactg accagctcct acgcaggcag aaaagagaga
     5701 aagagacagt tcagacagct gtggatcgca cgtatcaacg ctgcagcacg tatcaacggc
     5761 ctgtcctaca gcaagttcat gtacggctta aagctggctg aggttgattt aaaccgtaag
     5821 gttctgtccg atatggcgat caacgatgca gaaggcttcg caaagctggc tgagctggca
     5881 aagtccaagc ttgcttaatt aaaatatatt gataaaagct cctgttctat ggctgatgat
     5941 acagattcag ccgtggggca ggtttttttg tgtaaaaaaa tggataaagc aacatggaca
     6001 aggctgtttt gcagagttat aattgtataa aaatacacac aaaggaaacg aaagttatgg
     6061 cacagaatgt aaaagacagt acagacatga tccacggaag catcgccagg tccatcctgc
     6121 tgttttctat tcctcttctg gttggaaatt tcttccagca gctctacaat acagtagacg
     6181 cctatgtggt gggaaattat gtgaatacca gtgcgctggc ggcggtcgga gcatctacgc
     6241 cgatcgtaaa tatgctggta ggtttcttta cgggtctggc agccggtgcg ggggtggtga
     6301 tatcccagta ttttggcgga ggccggacgg agcagatgcg ccgggccatc cacagttccc
     6361 tggctcttac ggcaatcctg agcgttttgt ttacagcact gggcttaagc tttacaaagc
     6421 ctttgctcca cgccatcggc gtaccagagg atgtaatgcc taactctgcc ctgtatttat
     6481 ccatctattt ctggggaatg acattttcgc tcttttacaa catgggctcc ggtattctgc
     6541 gtgcgatcgg tgattctaaa aaccctttgt tttacctcat tgcagccagt ctggtaaata
     6601 ttgtcctgga ttttttattt gtctgcgggt ttggaatggg agtagaggga gcggcgattg
     6661 ccaccattat tgcgcaggcg gtcagcgcgg cgctggtgat gggaaagctt cttatgaccc
     6721 gtgagattta ccgtgtgcgg cttggagaaa tctgttttga caggaaaatg atccggcgta
     6781 ttgtggatat tggccttccg gcagcgatcc agcagagcat cacctcattt tccaatgtga
     6841 tcgttcagtc ctatatcaat tatttcggta cggcagccat ggccgggtac tctgctacgc
     6901 tgcgggtaga cggttttttg cagctgcccc ttcagagctt taatatgacg atcactacct
     6961 ttgtggggca gaatatcgga gcaaaggaat ataaaagggt aaagaagggg atttttactt
     7021 cctggcttat gtgctccggt attattttag tgggttctgt tatcatgttc ctgaaggcgc
     7081 cgtccatcat cggtattttt acagaggatg cggctgtgat cagcgtggga agcagtatga
     7141 tccgtatttt tggaagcgcg tatctgttcc tgcccatcat ccagatttta aatggcgccc
     7201 tgcgcggcgc aggacgctcc aaggttccta tgtatttcat gttaggctgt tttgtgcttc
     7261 tgcgtcagat ttatctgatg atcgccgttc ctatgacaaa aaatgtttcc gttgtttttg
     7321 caggatggcc tgtgacctgg gtggtctgcg ccatcggaat gctggtttat tattttaagg
     7381 tggactggct gccaaaggag gagtaaatct ggagattgaa aaaaatcgaa aatcccacta
     7441 cccaaatcct gcattttagt gtataataaa acacggcttg aaattaaagc cgtgtttttt
     7501 ggaggaatac ccaagtggct gaagggtctg gcttcgaaca ccagtaggtc ggcagtaccg
     7561 gcgcaagggt tcaaatccct tttcctccgt ttttgcaaaa taccgcttcc tgcagactcg
     7621 tgctgtatcc tgcaggaagc ggtatttatt tatgcataaa tgggctttgc aatcagaagg
     7681 ttccggctac agaggaaggg attgtaaact ccacagcgcc catatatccg ggagcaacct
     7741 catattcgtc aaaggcaatg accagaaggc cttcttcatt aaagtagaag ctctcatcgc
     7801 cggtaagctc cttaaagtca tcctccggca tgtcgctgtt gtaaaaatag cttacagaat
     7861 cgtcggctgc catctgggaa gccatctgtt ctttaatatt ggctgtgatg gaggagagca
     7921 tttccggccg gtccttaaaa agttctttca gggaaagctg tttcccggtg gttttatcaa
     7981 tggtaaagat ctttgtggac tgcgctccgc ttgccatgac aatggtggtg tcgatgcgca
     8041 ggcacagata gcggtcatta tccgttacaa ctttatagga ggagtccaga gaatagctgc
     8101 cttctccctg agactgggac agttcgcctt cataggcggc gatgagctcc tgtgcatatt
     8161 cctcaatgga tttattcaga gtttcgggtg cagcttcaat ctgcgggatc tggatgttgg
     8221 cctcaaagtt gtttgtctta ttttcgtagg tacgaaaggt tacaaccttt gcgatcgggc
     8281 cgataacagg gatctgttcc atggcattgg cgatggtcgg atgcagattg gtaagaagag
     8341 taatggcaac catagcggct gctgcggtca tgcctgtacg tttcattaat ttcataaaaa
     8401 tgactccttt catttgagct ttgtctgttt ctttttttcc ctgcgcgatt ccggcaagaa
     8461 tccggtctct ggcttccagg ggaacgggaa gtgactcata ttcctgtctg agctgttcta
     8521 actgtctgta tccggaaagt ttaaggtctt tctgctccat aaagattctc ctttcatttt
     8581 gcgtaagatt tcttcggttc ttcaggttcc atggtaattc tcagtttttt taaggctcgg
     8641 taaagacggg cttttacagt atttaaattt tcatctacaa ctctggaaat atcttccagt
     8701 ttcatatctt caaagtaccg cagcatgaca atggttctgc ttttttcatc cagatgcttt
     8761 agggcggtct taagatccag ctcctgatat ttgtcctccc atgggatttc tggcatttcc
     8821 tctgcgggga tttccttttt attttttctc agaagatcca gagaggtatt tataacgatc
     8881 cgatagatcc atgtggataa aaagtctgta ttttttacct ggccgcagtc ccggatggct
     8941 ttgcaggcgc tttcctgaac gacatccaga gcatcctccc gattctgtac ataactgtag
     9001 gccagacggt aaaagcgctc atatccatcg atcagtatct gctcggtctg ttttgtgatg
     9061 gggttggtat ccacgttaaa cttctccttt cttggtttct gtctgttaga cggcgggaat
     9121 ggtgaaaaag ttgcattcag taacaatgaa tttattgcat ggctgctttt attgtatacg
     9181 ttaaggagcg aaagaatcaa cgcccgtaca ggaaattttc tgattcgccc tgtattgaat
     9241 ttttataagg gaaagggtat aattaacata actgatatat ttaagcagta agagtgtatc
     9301 agataaaatg ctgcatttga aaaaaagggt aaggagaatg agaaaatgat cgagtggaag
     9361 aattttgaca gtcttgagtc ctacggggag cttgccaaag cagagcgtgt taagcttgca
     9421 gaagtaatgt ccggagagag cggtgcagag cgtgtaaaag catacagcgt tcccatggca
     9481 gcaggcatgg tctataacta tgcggcaaag caggtggatg aaaatgtgct tgcagtatta
     9541 aaaaagctgg caaaagaagc gcagctgtca gaaaaataca aagctctgta caatggggag
     9601 atgatcaata caggagagaa gcgcctggtg cttcaccagc tcacacgcgg acagcttgga
     9661 gataaggttg tggcagacgg cacagacaag cgggctttct atgtagagca gcaggagaga
     9721 atcgctgaat ttgcaaagaa ggtacacagc ggcgagatca ccaatgcagc aggtgagaag
     9781 tttacaacgg ttgtacagat tggaatcggc ggaagcgatt taggcccccg cgcaatgtat
     9841 ctggcactgg agaactgggc aaagaaacat aatacattta aaatggaagc aaagttcatc
     9901 agcaatgtag atccggatga tgcggctgca gtgcttggtt cgattgatgt tgctcattct
     9961 atgtttgtgc tggtttccaa gtcgggtact acgttggaaa ctctgacaaa tgagtccttt
    10021 gtaaaggatg ctttaaagaa ggcaggtctg gatgcctcta aacatatggt tgcagtaaca
    10081 agcgagacct cacctctggc aaagagtgat gattacctgg cagccttctt tatggatgat
    10141 tatatcggag gccgtttctc atctacttcc gcagtaggcg gggcagtcct gtcactggca
    10201 ttcggaccgg aggtgttcgc tcagttctta gagggtgcgg cagaggaaga taagctggca
    10261 gcaaaggaag acctgctcca gaatccggat atgctggatg cgctgatcgg cgtatatgag
    10321 agaaatatgt taggctatcc ggcaacagcc gttctgcctt attcacaggc attaagccgc
    10381 ttccctgcgc atctgcagca ggcagacatg gagtctaacg gaaagtccgt gaaccggttt
    10441 ggtgagcctg taaactatgt cacaggcccg gtcctcttcg gcgagcccgg aacaaacgga
    10501 cagcattcct tctaccagct gctccatcag ggaaccgata ttgtgccgat ccagtttgtg
    10561 ggatttaaga acagccagat cggaacagac gttgtgatcg aggacagcac gagccagcag
    10621 aagctctgtg ccaatgtggc tgctcagatc gttgcatttg cctgcggcaa agatgatgag
    10681 aatctgaata agaagtttga gggcggacgt ccctccagca ttatcattgg agagcagtta
    10741 aatcccaagg cacttggagc actccttgca cattttgaaa ataagatcat gttccagggc
    10801 tttgtatgga atgtaaacag cttcgatcag gaaggcgtac agctgggcaa ggtacttgca
    10861 aagcgcgtac tggcccatga aacagacgga gcgcttcagg tttacagcga gcttttgaac
    10921 atctgatcaa acagaagaaa aaatatactt gcgaagagaa aaaccgtccg ttatactgat
    10981 agtatcagtg agggcgcagg tctggtttgg ggattccatt cctgtctttc acggaaagac
    11041 gggttacaga tatgaaggat atcatggatg aagataagag actggaagaa gagctcaagg
    11101 agctgcgcca ggttcccata gacgaaaatg acagaaaaaa gaaaaagagc cgcagaagcc
    11161 gcaaaaaaac ggcaaaacgc aggggatttc cctttgcagc cgtatttatg gctcttgtgg
    11221 taggcgttct ggcaggactg ggcggcggtt actacgtatg gggctgggaa cggccctata
    11281 cggtagatct taaggcagta gaggttccgg attgggtaaa gcaggagttt atccggaaaa
    11341 atattttttc aaggccggat gtgagccgca ggaagatcaa caatattgtg cttcattatg
    11401 tggcaaatcc cggttccagt gcagagggca accgaaatta ttttgacagc ctggcggatc
    11461 aggacagcca gaaaggtggc acctctacca gcagccattt tattgtgggc ctggaaggag
    11521 aggtgatcca gtgtatcccg accaatgaga ttgcctacgc aaatgctccc agaaacgagg
    11581 atactctttc cattgaggtc tgtcacccgg acgatacggg aaaatacagc gatgctacat
    11641 atgagtctgt cgtgaggctt acagcctggc tgtgtaccca gttaaagctt aaaagcagcg
    11701 atgtggtgcg ccattatgat atcaacggta agctgtgtcc gaaatattat gtggagcatg
    11761 aggaagagtg gaagcagctg agaaaggaca ttgatgcggc catgaaggag ctgtgatctg
    11821 cgatactgca taaaaaagcg gggagatccc tacaggggga tcatctccgc ttttcgatta
    11881 tggtaaatcg tatagtaatg aaagccgcac tgctttaaaa gctcctttgc gcgggtaaat
    11941 ccatatccca gatgctccgg agcatgggcg tcagagccga tggtgatgtg ttcgccgccc
    12001 atcctgcggt attgctttaa aatctctgta tcggggtttt ggtgactcag cctgctgcgg
    12061 aagccgcccg tattgcattc cagagccttt ccattttcag ccaaaaccct caaaatagaa
    12121 tcaatctgct ctttaaaggc aaaatagctg tagacagaag cactgtccgg agcatagcgt
    12181 gcggcatagt ccagatggcc ccaggagtca aagaatggat gaaaggctct ggcgcgctta
    12241 aaggaaaggg tgaaaaactc ctcaagtccc tgcctggaac cgcaggcttc ccaaaaggag
    12301 ggataaaagg gatcatggga gtggataaaa tggctggaac cgatgataaa atcaaattta
    12361 gagccaaaat ccttatgaaa tttttccagg tacctgcaga tgtgaagctg cagccccagt
    12421 tcgatgccga tattgatgcg gattttatcc cggtattctt ctttaagagt gttcagggcg
    12481 gacagatacg agggaagatc cagctcaaag ttatcctcac agaaggatga gtcgcggtca
    12541 tgatggtccg taatggttat ttccttcatt ctcatagaaa cggcccgttc aatctgaaca
    12601 cgggcgggtg tatttgaatc gccggaaaaa gcggtatgca gatgacagtc gcagatcatg
    12661 caggctcctc ctttcaggcc ggatgccaaa ggcgcggcgt ttgatgataa atattatacc
    12721 tgcggcagat aaaaagttca aggctgacaa acaaattcag gggaaagagg taaaatgctg
    12781 taatttcccg cacaaattgt tataaaatta acgaaaaggt agaattaaaa aaaacgcttg
    12841 ctattttgga aaaaattgat tacaatgagt acaagttgat tattatttaa caataataca
    12901 atttaacaac taggaggaat tgaatcatgg cagtaaaagt tgcaattaat ggtttcggac
    12961 gtattggccg tctggcattc cgtcagatgt tcggcgcaga gggctacgaa gtagttgcta
    13021 ttaacgatct gactgatcct aagatgttag cacatctgtt aaagtatgat accgctcagg
    13081 gcggatacgc tggccacatc ggcgagggca agcacactgt agaggctggt gaggattcca
    13141 tcactgttga cggaaagaag atcacgattt acaaggagcc aaaggctgct gaccttccat
    13201 ggggagagat cggtgtagac gtagtattag agtgtaccgg tttctactgc tctaaggata
    13261 aggctcaggc tcatatcgat gcaggcgcta agaaggtagt tatctccgca cccgcaggca
    13321 atgacttaaa gaccatcgta ttctccgtaa atgaggatac tctgactgct gaggatacta
    13381 tcatttccgc agcttcctgt acaacaaact gcctggctcc aatggctaag gctctgaatg
    13441 attacgctcc tatccagagc ggtatcatga gcaccatcca cgcttacaca ggcgatcaga
    13501 tgatccttga cggaccacac agaaagggcg acttaagaag agcacgcgca ggcgctgcaa
    13561 acatcgttcc taactctaca ggtgctgcta aggctatcgg cctggttatc ccagagctga
    13621 acggcaagct gatcggttct gcacagcgtg ttcctgtacc gaccggttct accactatct
    13681 taacagcagt tgttaagaag gctggcgtta caaaggaaga catcaacgca gctatgaagg
    13741 cagcagcttc cgagtccttc ggctacaacg aggatccgat cgtatcttct gacgttatcg
    13801 gcatgaagta cggctccctg ttcgatgcta ctcagaccat ggtagctcag attgctgagg
    13861 atctgtatga ggttcaggtt gtttcatggt atgacaacga gaactcctac acaagccaga
    13921 tggtaagaac cattaagtac tttgcagagc tggcttaatt attcagtttt ttagtagact
    13981 cataaaaagg gtccggtcgt cagggccggg ccctttttac gcagtatgaa aggggtacat
    14041 ccattatgtt aaataagaaa tctatcgacg atatgaacgt aaaaggcaga agagtgctgg
    14101 tgcgctgcga tttcaacgtg ccgttaaaga atggtgagat caccgacgag acacgtatcg
    14161 tggctgctct gccaaccatc aacaagctga tcgctgacgg tgcaaaggta atcctctgct
    14221 cccacttagg caaggtaaag aatggcccta acgagggtga gtctttagct cctgtcgcaa
    14281 agagactgag cgagaagtta ggcaaagaag ttgtattcgt agcggattac aacgtaaccg
    14341 gcgaggcggc aaccaaggct gtagaggcca tgaacgaagg cgatgtggtt cttcttcaga
    14401 atacccgttt ccgcggcgct gaagagacaa agaacggcga ggcattcagc aaagagttag
    14461 cagatctggc tgacgattat gtatgcgacg cattcggttc ctcccacaga gcacacgctt
    14521 ccgtagaggg cgtaaccaag ttcatttctg ccaagggcgg cacaaatgca gttggctatc
    14581 tgatgcagaa ggagatcgat ttcctgggaa atgcagttga aaatcctgta agacctttcg
    14641 tagcaattct tggcggtgca aaggttgctg ataaattaaa tgttatctct aatctgttag
    14701 agaagtgcga tactctgatc atcggcggcg gtatggcatt tactttctta aaggcagagg
    14761 gcaaggaaat cggaaagtcc ttagtagatg ataccaagtt agattactgc cgtgaaatga
    14821 tggcaaaggc agagaagctt ggcaagaagc tccttcttcc tgtagatgca gtagtagcag
    14881 cttccttccc ggatccaatt gatgctcctg ttgaggcaga gaacgtatcc gtagatgcta
    14941 tgcctgctga taagatgggt ctggatatcg gcactgagac tgcaaagatc tatgctgagg
    15001 ctgtaaagtc tgcaaagact gttgtatgga acggaccgat gggcgtattt gagaatccaa
    15061 ctctggcaaa gggaaccatc gcagttgctg aatctctggc tgagacagat gcaactacga
    15121 ttatcggcgg cggcgattct gcagcagctg taaatcagct gggatttgct gataagatga
    15181 gccacatttc caccggcggc ggcgcttctc tggagttcct tgagggcaag gtacttccag
    15241 gtgtagcagc agcagatgac aaataaggct gtatagcata agtataccgc ccggaccgga
    15301 gataagcctg cggtccgggc ggtccattta cggaatcaat gtgtgtaaag gagagataat
    15361 catggcaaga aagaaaatca ttgcaggcaa ctggaagatg aatatgaccc cttctgaggc
    15421 agtagcactg gtaaacgagt taaagccttt agtagccaat gatgaggtgg atgtagtgtt
    15481 ctgtgtacct gctattgata ttatcccggc aatggaggct gcaaagggaa gcaatatcaa
    15541 tatcggtgct gaaaatatgt attttgagga aaagggagcc tatacaggtg agatttcacc
    15601 caatatgctg acagacgcag gcgtaaaata cgtgatcatc ggacattccg agagaagaga
    15661 gtattttgca gagacagatg agactgtaaa taaaaaagta ttaaaggcat ttgaacacgg
    15721 tattaccccg attatctgct gcggcgagtc tctgactcag agagagcaag ggattactat
    15781 tgactggatc cgtcagcaga tcaagatcgc tttcttgaat gtaacagcag atcaggcaaa
    15841 aactgcagta attgcatatg agccaatctg ggccatcgga accggaaaag tagctaccac
    15901 agagcaggca gaagaggtat gtgcagctat ccgtgcctgc atcggtgaaa tttatgatga
    15961 agcaactgca gaagctatcc gcatccagta cggcggttct gtatctgctg catctgctcc
    16021 cgagctgttt gcacaggcaa atatcgatgg tggtctggta ggcggcgctt ccttaaagcc
    16081 tgatttcgga aagattgtaa attataagca ggcgtaagcc aataaggaga taaacgatta
    16141 tgaataagaa accagtagtt cttatgatcc tggacggata tggtctgaat aaaaactgcg
    16201 atcacaatgc agtttgcgag gctaagactc cggttatgga tcagttgatg agccagtgtc
    16261 cttttgtgga aggtcaggcc agcggcctgt ttgtaggact tcctgatgga cagatgggta
    16321 attctgaggt aggccactta aatatgggtg caggacgtgt ggtatatcag gagctgaccc
    16381 gcattaccaa gtcgattgag gacggcgatt tcttccaggt tcctgaattt ttacaggcag
    16441 tagaaaactg taaaaagaat gattccgctc ttcatctgtt cggtctggta tctaccggcg
    16501 gcgtacacag ccatttaacc catatctatg gcctgttaga actggcaaag agaaatggcc
    16561 tggagaaggt atatgtacac tgcttcctgg atggacgtga cacacctccg gaatccggta
    16621 aggattttgt ggctcagtta gaggaaaaga tggctgagat cggcgttgga aaggtagctt
    16681 ccgttatggg acgttactat gcaatggatc gtgataagag atgggatcgc gtagagctgg
    16741 cttacagagc acttacaaag ggtgaaggta aggcagctga gagcgctaca gccggtattc
    16801 aggcgtctta tgatgatgga aaggcagatg aatttgtagt tcctttcgtt gtaaccgaaa
    16861 acggtgctcc cagagcttcc atcaaagatg gcgactctgt tgtgttcttt aacttccgcc
    16921 ctgatcgtgc aagagagatt actcatgcat tctgtgacga tgagtttgac ggctttgcaa
    16981 gagagaaaaa actggatctg gtttatgttt gctttacaga ctatgacgag accatcacaa
    17041 ataagctggt ggcatttaag aaggagcaga tcaccaatac cttcggagag tttctggctg
    17101 ctcacggcaa gaagcaggta cgcattgccg agacagagaa gtacgctcac gttaccttct
    17161 tctttaacgg cggagtagag gagccaaatg aaggagagga tcgtattctg gtgccttctc
    17221 caaaggaagt ggctacctat gatttaaagc ctgagatgag tgcaaacggc gtatgcgaca
    17281 aactgacaga ggctattcgc tcaggaaagt atgatgtagt gatcattaac tttgcaaatc
    17341 cggatatggt gggacataca ggtgttgaaa atgcagccat caaggctatt gagacggtag
    17401 acgcctgtgt aggaaaggcc gtagaggctg taaaagaagt ggacggtgta atgtttatct
    17461 gcgcagacca cggaaatgca gaacagctgg tagactatga gacaggtgag ccattcactg
    17521 ctcataccac aaatcctgtt ccttttatcc tggtaaatgc agatccatcc tttaaacttc
    17581 gtgaaggcgg cgctctttgc gatatcgcac ctaccctgat tgagctgatg ggactggagc
    17641 agcctaagga gatgactggc aagtctctgc tggtgaaata agataaaatg ataccgcttg
    17701 agagtggctg acaattcccc cccggagaga gcatatcaga agcttttccg gggggttttg
    17761 cagtagtacg cctgaggact cagactttaa ttattcttca tctgcagccc gatgaccccg
    17821 atcataaaag cggctgttcc gccgatgatc cagccagtga ccgtttcgtg aaagaagaat
    17881 acgccccaga gaatggcgaa tacggccatc atgctctgga tcacgggaac catataaaag
    17941 ggcaccaggg ggatggcttt ggcattgagg taaaagccca ttcccgtgat aaagccgaag
    18001 gcaaggatgg caaggatgca ggccagatcc ggacggataa aagctatttt ccctgtaaat
    18061 acaggctctg cggccgggat cagggaaaaa agaccggaca gggtaaatat agccagattg
    18121 ctgtcgataa tatccagccg tccagctacc attttctggg aaagcacatg gcagcctgca
    18181 cagataccgg acaggataaa caggccggta aaaaggagat tttctttaaa aaatacatcc
    18241 agaggacggc cgtttaaaga gatgcacaga acgccgccga tgcagagaaa gatacagaat
    18301 ccctttctgg gggacagctt ctccttaaag cacagtacgc ttaccagcgt cagaaacagg
    18361 gtctgggcag gctgggtcac aatatttcca taggaaagtc cgtggcttaa ggcgtagttt
    18421 tctgtcagat aggccatcca tttgataaag gctccgaaca ggatccattt ccagcaggat
    18481 aagagggcct gttttaagtt tttttgaaat ggctgcctca ggatacactt taagatgagc
    18541 agccacacca ctccaatgaa aaaacggaaa aaggtaatcc atgaagcatt aaaataaggg
    18601 ctgatcagtt tgacgcaggt accgccgaag ctgaacatca gggcaacgca gagcagatag
    18661 acatatccca tagctgattt tctccttcaa atctatttga cactattata gcaaaatctg
    18721 gaaaaaaggg taggaaaaaa ttctttcccc tgcctcttcc attttctatt cagatacgtt
    18781 acaataaaaa gataactgcc cggagaaatt actctgatgg caagaacaaa acaggggaga
    18841 aaggaatatg aatacgaaaa agggaatgaa agcggcaaag ctgttaaaaa gctttcttcc
    18901 atattatgga aagtacaaaa aggtcctgat cctggatctg ttctgtgcgg cattgaccac
    18961 gctgggagag ctggtgcttc ctctgatcct tcggtttatc accaatcagg gaatgcagga
    19021 tctggcttcc ttaaccacag ggcttgtggt aaaaatcggc cttttatatc tgatcctgcg
    19081 ggtgatcgat tcgctggctg cctattatat ggcatatacg ggccatgtga tgggcgtata
    19141 tattgaaaca gatatgcgcc aggatgcctt tgagcatctg cagatgctct cggacagcta
    19201 ctacagcaat accaaagttg gacagatcat gtccaggatc accagcgatc tgtttgacgt
    19261 gacggaattt tcacaccact gtccggagga attttttatt gccattttaa agggaatcgt
    19321 atcctttgtg attttgtcag gaatcaatct ggagctgacg ctgctcattt tcctgatgat
    19381 cccgatcatg atcgtttcct gtacctggtt taatctgaag gtaaaagcgg ccttccggct
    19441 tcagagaaat cagatcggcg agctgaacgc ccagattgag gatacgcttt taggaaaccg
    19501 ggtggttcgt gcctttgcaa atgagccggt ggagattgaa aaattcggaa aaggcaaccg
    19561 ggagtttatg aagatcaaaa agcataccta ccggtatatg gcggcctttc agattaccac
    19621 ccgttccttt gacggcctga tgtatgtgct ggtgatcgtg gccggaggcc ttttcatgat
    19681 taacggaaag atcgcgccgg gagatctggt cgcctatacc atgtacgtga ccactctttt
    19741 gacaaccatc cgccggatta tcgagtttgc ggagcagttc cagagaggaa tgaccgggat
    19801 tgagcgtttt caggagatta tggatgtgac tcccgatatc agggataaaa agggagcaaa
    19861 gcctctttcc gatgtaaagg gaacaatcga atttgaccat gtaagctttg aatatgcaga
    19921 cgatcatacc caggtactga atgatatctg tttaaaaatc cgtccaggcg agaggattgc
    19981 tttagttggc ccttccggcg gcggaaaaac tacactctgt aatctgctgc ccagatttta
    20041 tgacacaaca gggggaacta tctccattga cggaatggat gtaagggata ttaccctgag
    20101 aagcctgcgc tccaatatcg gagtggtaca gcaggatgta tacctgtttt ccggcactgt
    20161 atttgaaaat attctctacg gacgccccgg cgcttccaga gaggatgtga tcgaggctgc
    20221 aaaaatggcg ggggctcatg agtttattac ggaattaaaa gacggctacg atacctatgt
    20281 gggcgaacgg ggcgtaaagc tttccggcgg gcagaaacag cgcatcagta ttgccagggt
    20341 atttcttaaa aatccggcaa tcctcattct ggatgaggcc acctcagctc tggacaatga
    20401 gagcgagtat ctggtatcac agtctctgga aaagctggct gagggccgca ctacgttaac
    20461 tatcgcccac cgcctgacga ccattcaggg ggcagaccgg atcctggtgc tttccggcaa
    20521 tgagatcgtg gaggaaggaa atcataagga acttcttgaa aagaagggga tgtattacca
    20581 gctttatatg acagccaacc gtttgaatca ggggcttaac tgaaaggagg atatgttatg
    20641 aaaatagcag cagctacgga cagcaacagc ggcattaccc aggatcaggc aaagcagctg
    20701 ggagtccatg tgcttcccat gccctttatt attgacgggc agatgtacta tgagggtgtg
    20761 gatcttaccc acgaggaatt tttcagacgg atggaggagg gagcggatat taccacctct
    20821 cagccgtccc ccggtgaggt gactgatttc tgggataaac ttttagagga gtatgatgca
    20881 gtggtgtata tccccatgtc cagcggttta agcggttcct gtcagaccgc catgctttta
    20941 gcagaggatt acgagggaaa ggtatatgta gtcaataacc agcgcatttc cgtgacccag
    21001 atgcagtctg tgctggatgc cagagatttg ataaaaaagg gatatgcggc tgcacagatc
    21061 cgcaatattt tggaagaaac aaagtttgac agttccatct atgttacagt gaccacttta
    21121 aaatatctta aaaagggagg gcgcatcact ccggcagccg ctatgttggg aactcttctc
    21181 cagattaagc ctgtcctcac catccagggg gaaaagctgg atgcattttc caaggccaga
    21241 accttaaagc agggaaagac cattatgatg accgctctta aaaaggatct ggaaacacgt
    21301 tttcacgatc ctgaggcacg tcatacctgg ctggagattg cttatacctg cagcgatgag
    21361 atggctcagg aatttaagga aacagtggca gaaatctatc cggatgccaa tatctgcata
    21421 gcgccgttgt cattaagtat tgcctgccat atcggaccgg gctgtcttgc tgtggcctgc
    21481 agcggccatt taaaggagct ggagtagatt gaaaaagaag aaatggcttg agcggtttaa
    21541 gctaagggga aaaagcgtca tgatccctat ggtgattctg atgatcctta ctgctttggt
    21601 tcccatgctt ttgcaggcaa tgatcagccg cagggccttt aaccagaccc agctggaggc
    21661 gaagggcatt gagatacaga atcagtgtct gatcttaagc agccgcctca tgcgttcagg
    21721 ttatatgacc tcggataagg gaaataccat ggagctggat acccgtatgc agacgatcgc
    21781 agatatttat aacgggcgga tcgtaattgt aaacagcagc tttaagatta tgaaggatac
    21841 ttttaacctg gcggcaggga agtattatgt ttcagaagag gttatccgct gctttaacgg
    21901 ggagacaaac agccgtatga atcaggagat gggttatctg acccagacta ttcccatcca
    21961 cagtgcttcc gataaaaaga aggttacggg agtgatggtg gttatggctt ctacggaaaa
    22021 tatttccgca ctgaccgacc gggttcaggg aagcacccag ctgtttttgc tgtgcatgat
    22081 cctggtggtg gtggtctgtt ccgtggcagc cgtgtttttc cttatacggc cttttaagcg
    22141 gcttctggct tcctttgagc gtgtttccta cggagatttg gacaccgata tcatggagga
    22201 tgcctataag gaaacaatcc agctttcaca ggcagtacag aagtcgctca gaaagcttaa
    22261 ggcagtagat cagtccagac aggagtttgt ttccaatgta tcccatgagc tgaagacccc
    22321 gatcacctct atccgggtgc ttgcagattc gcttatgagc atggaagaag tgccggcaga
    22381 gctgtaccgg gagtttatgg cagatatttc ggatgaggta gaccgggaaa atcagatcat
    22441 cactgatctg ttaacgcttg tgaaaatgga taaatctgcc cagagccaga tgaatattac
    22501 acagacgaat ataaatgaag agctggagct gatcttaaag cgcctgcgtc ccattgccaa
    22561 gcgaagcaat gtagagctaa ttttagaaag tatcagagag gtaacaggag atgtggatcg
    22621 ggtgaaattt tccctggctg tatcgaacct ggtagaaaat gctattaagt ataatgtgga
    22681 ttccggctgg gtgcgggtca ctctggatgc agatcataaa tatttttata ttcgtgtagc
    22741 ggattccgga atcgggattc ctcaggactg catggatcat atttttgaac gttttttccg
    22801 cgtggacaag gccagatcca gagaggtggg aggcacaggc cttgggcttg ccattacgct
    22861 gaatgtgatc cagatgcatc atgggatcat tgatgcggaa agtacaccgg gagaagggac
    22921 aaccttcagt gtgcgcattc ccctaaatta tgtgtccaga caggaggata aatcatgagt
    22981 accacaggaa aaaagggaag attctcagta aagatccttc tgagactgtt ctgtctgaca
    23041 gggattttat tcctttccgg ctgtcatctg gagaaggagc ctgtggcagc tccgaaggaa
    23101 ggacgatatc tggtttacta tctgaatggt tccggggtaa atctgattcc cagggtgtat
    23161 gaaacggagg aaacagatcc gcttgccctg tctgatgagc tgatggagca gctgcgttac
    23221 gtccctaagg atctggactg ccagactgct ttgggagaca aggcagtatt cagggaaagc
    23281 cgtctggacg caggggtact gtcgctgtat tttgatgtga gttatgtaaa tatgaagcca
    23341 gagcaggaaa ttttatgccg cgcggctctg gcgaggacgc ttacccaggt accgggcgta
    23401 gattatatca gtatttactg tggagatcag ccgctgatgg atggtcaggg aaatccggta
    23461 ggcgctgtgt ctgcctccga ttttatatta aataccagta atgtaaattc ctatgaaaaa
    23521 acagagctga agctgtattt tgcagataaa acgggaacaa agctgatctc tgagacaaga
    23581 gaggttgtcc acaatataaa tacctccatg gagcagctga tcgtggaaca gcttttagca
    23641 ggtccggcgg aagaaggcca tctggcggtg ctcccatctg actgcaagat tttaagcatt
    23701 tctgttacgg ataatgtatg ctacatcaac tttgattcct cttttttgaa aatggagcat
    23761 ccagtaaatg aatatcttcc catttatgcc attgtctctt ctctggctga gatgacaagc
    23821 attacaaggg ttcagatcat ggtaaacggt tcccaggatg tgatgtttcg ggatgtggta
    23881 tccttaaacc agacctttga gttaaaccat gagtatatcc aagaagaata atgaggtaaa
    23941 atgaatatga aacggccgat tgcaggtatt gcagcaggtt tcgtacttgg agaggtgctg
    24001 gtcctgcaga tgcaggaggc tgtgaatgtg tggctgacgg tggtcctttt gataccggca
    24061 cttttttgtg tggttttaaa atgggggacg gcaaaagaaa gacggacagg caggagtggt
    24121 attaaaaaaa agctcctgtc tgttttttta attagtttgg ctatggcgtc gggaagcggc
    24181 agaggaggta tggtaaagaa ccggttggat catgaggaag gacttgtaaa gccctttgag
    24241 ggaaaacagt gccggatcag aggcactgtg gaaaaacgaa gctggaagga agaacggctg
    24301 acactggagc ttaaagaatc agagctccaa agcggagaga ggaaggaaac gtttcgccgt
    24361 atactgttgt atatggatta tggcaatgtg ccggactgg
//
LOCUS       DBMDUH010000022        24289 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_1386_length_24289_cov_5.890152, whole genome shotgun sequence.
ACCESSION   DBMDUH010000022 DBMDUH010000000
VERSION     DBMDUH010000022.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 24289)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 24289)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24289
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1386_length_24289_cov_5.890152"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="ACZPCG_03450"
     CDS             complement(<1..226)
                     /locus_tag="ACZPCG_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HIR1284161.1"
                     /translation="MNKKNSSWKAGIQTFGRSLLLPIALLAPIGMVMGICSAMGQSYM
                     IDKFPFLGNEIVRLIFSSLQSITSIIFNNIP"
     gene            443..1990
                     /locus_tag="ACZPCG_03455"
     CDS             443..1990
                     /locus_tag="ACZPCG_03455"
                     /inference="COORDINATES: protein motif:HMM:NF016901.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1284162.1"
                     /translation="MGFNYNDLTLLRFIQKRHSIPLAKATLQFGKNETSIRRAVEQIN
                     LSFPSPVIEIEKGICSSRLSYEELVSLIRSMSPSDYASSSSERIMVMIVTIFFCGYVN
                     ASELYEKWGLSLTTKKQDTGQLRSFLKKYRLFLTTLHKKGLTITGDELQLCFLVIDIL
                     HPLLEFTPENRVHARFANTPIERQSFETAMIYLEKGFSPAAEKLTHFLEENHLALNYP
                     SKKFLLLFICIMETRPLKPDTTFSCRLPFKPLDLHFTDSPMENKLYNVALSMQNFSKN
                     PTLPFDARLWQITERFAEDVVNGLAHPFSISEDFLTELYEYFYREITLDYFHCTFVDK
                     TVENTAGKFPVLYEQIRRYGIYFQAAYNFSLLDEHLSTLTLMVEKHIIKNRTADRRRK
                     RIIIMTSINFERISFFLEQLREYIAFQWVETLNLNEIHRLNDLSYDCIFCFSTRIFNI
                     LNSRELPVIRVNFFLENKDIDRLLKLGFSAQTHRFNANSLALDLAGKTEAEMVSYLKN
                     RFGDYFV"
     gene            complement(2006..3256)
                     /locus_tag="ACZPCG_03460"
     CDS             complement(2006..3256)
                     /locus_tag="ACZPCG_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003510739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HIR1284163.1"
                     /translation="MVKAQETNSRGFLQVNVVNIQNNFPIKNATISISTKGEPDRVME
                     EVSTNSSGQTEQLSLPAPPVEYSLNPGIIQPYSEYDLIVRAEGFEPLAISGTEILADS
                     NAIQPAAMTPLAQEQPPEDPVVIPDHTLYGNYPPKIAEDEVKPVNESGEIVLSRVVIP
                     QTIVVHDGVPTDSTAKNYYVPYRDYIKNVACSEIYSTWPESSITANVLAIMSFTLNRV
                     YTEWYRNQGYDFTITSSTAFDHKWIYGRNIFQSISQVVDEIFDAYLSRPGVRQPILTQ
                     YCDGRQVSCEGWMTQWGSCDLGKQGYSPIEILRYFYGDSIYINTAEQISGIPASWPGS
                     DLSQGSSGSKVRQLQEQLDAIATVYTAIPRVAPDGIYGPRTAAAVREFQSIFGLPQTG
                     AVDFATWYRISHIYVGITRMAELI"
     gene            complement(3357..3935)
                     /locus_tag="ACZPCG_03465"
     CDS             complement(3357..3935)
                     /locus_tag="ACZPCG_03465"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002593945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HIR1284164.1"
                     /translation="METQKINTKKQILRKQIREKNAHLDPEYCLWADKRIREWILNWK
                     YFQKAETIFCFVGTAGEIHTKPLIQAALDQGKRVAVPKCISKGIMEARQIRSLGELYP
                     GSYGIEEPEKAAEIIGPEEISLALVPCLTCSEDGRRLGYGGGFYDRFLRSVPGPWAVL
                     CRGRLMEAEIPWEAHDLLMDVVIWEEGILELK"
     gene            complement(4065..6080)
                     /locus_tag="ACZPCG_03470"
     CDS             complement(4065..6080)
                     /locus_tag="ACZPCG_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HIR1284165.1"
                     /translation="MTAKELFERGNEYFFFDDPEKTAAVCRERYPEDCAHIIRVADEV
                     CRNYFLFDLDHDLERTWEPVTFPEDRPIDWEYRPGNDPEFTFQFNRHRFLICLGQAYW
                     LTGDSKYEHHFFRLLMDFIDHVKRTPETEQTTWRILEVGIRGENWVKALRYFKDSPLM
                     TDERAEKIECCLREHGEYIIQMHSPYRYISNWGVMENHGLFEIGIQMPDQETRDRFTS
                     IALERLETEAAMQIFDDGVQWEQSPMYHNEVFHCYEDVLLLAKRNGIAVSERICDAVR
                     RMAYANLAWMRPDHRQIIMGDSDDMDLRDYMSIAAYLLKDPVLKFGGFKELDFESLWD
                     VGVNGAAEYSRMECRKPDFVSAALEDSGNYYIRSSWNEDGDLLHFHCGTMGAGHGHSD
                     KLHVDLILGGEDILTDSGRYTYVMDRGRFEFKDPDAHNTVTVDGEFFTQCKDSWECSK
                     LSQPVKQQYKQKNGYSLVQGGNLGYMHKGVFVNRKVISLKDGLFVIADELYGQGKHTF
                     EQFWNFSETGKAVYENGCACFTGEKAQVKLLFISPDCEGEMKQGKIARHYNRYETRDR
                     LCLQKKGQGFQSMITVIGGGKKGKERQLHASLVPVTSVLKGITYPPSMAEAVEIVDGS
                     RTLVVILCHQEVNSPTDLTEAAGCMGYGNVIVFDKAEGSVGGTVLCY"
     gene            complement(6121..7302)
                     /locus_tag="ACZPCG_03475"
     CDS             complement(6121..7302)
                     /locus_tag="ACZPCG_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007715080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HIR1284166.1"
                     /translation="MSWISWDALEKMPELTKEEAGRALDAACSQVIRNLDQFTDHFQK
                     AYSENGFYAPTENVDWTPGFWTGEIWLAYEHTGNEALKKAGELQMASFLKRINEKQDV
                     ETHDLGFMYSPSCVAGYKLTGSRVGREAALKAAEELITRFHEKGQFIQAWGPLDAPDN
                     YRLIIDCLLNLPLLYWASQETGDPRYKEIAERHIHTALSNVIRPDFSTWHTFFFNRET
                     GEPDHGATCQGYRDGSAWARGQAWGIYGTAIGYKYTKRESYIPVFEGVTNYYLEHLPE
                     DLVPFWDLEFGDGDDQPRDSSSASIAACGLLEMAKYVEPEKGALCRSLAARFMKSLTE
                     KYAVKDFDSSNGLVLHSTYSNHSPYNTCNHYGVDECNLWGDYFYMEALTRMYKDWNVY
                     W"
     gene            complement(7345..8181)
                     /locus_tag="ACZPCG_03480"
     CDS             complement(7345..8181)
                     /locus_tag="ACZPCG_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007715084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1284167.1"
                     /translation="MKIKNTKTRMMRAALYIVLIVLAFLMLVPFAWMLSASFKLDKDV
                     FIFPIQWIPTNPRWQNYVDIWTRIPLLKFVLNTVKLTLIVTFLQLLTSSFAAYAFSKL
                     NFKGKHVLFLAYIATIAVPWQVYMVPQFMMMRSMGLADTHLAIICLQAFSAFGVFMMK
                     QFYEGIPTELCEAARIDGMSEYRIWYRVMLPLAKPALSTLTIFTFVNTWNDFLGPLIY
                     LTTESKKTLQLGLRMFISQFGSEYGLIMAASVLSLIPVLIVFLSLQKYFVEGVAASGV
                     KG"
     gene            complement(8197..9270)
                     /locus_tag="ACZPCG_03485"
     CDS             complement(8197..9270)
                     /locus_tag="ACZPCG_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006779174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1284168.1"
                     /translation="MGKTKAVDPARVREKLDILEPRLAALLKQADEETDMKKKVKLAK
                     KADQIRDKIRQAKTGERFSRQTKRDLIAYSFIAPNFIGFAVFTLGPVLFAFILAFFKW
                     DGNNPMDFVGLDNFMQMVGNKRFLASLKNTVVYCVATVPFTLAVALGLAVLLNQKVKG
                     RNFFRTVSFFPYVASLVAVAAVWNMLFSPAKSGPVNMILYNLGVAAKELPKWSADKDW
                     VMFTVILFSVWKNMGYYMVIYLAGLQGINGELYEACSLDGANTWQRFRYITWPQLQPT
                     TFFVTIILTINCFKVYDIVYMLAGGSTGVLNESAIVLVYHIYEEAFRNWNLGYASAVA
                     MVLFLIVLAITLVQFRGEKKYAN"
     gene            complement(9354..10706)
                     /locus_tag="ACZPCG_03490"
     CDS             complement(9354..10706)
                     /locus_tag="ACZPCG_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006777508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1284169.1"
                     /translation="MMKKRIVSLALAVSMAGSLTACGGGAAKTENKETEAAADTTAAK
                     AEETEAAADGKTHLKWAMWDKDLTVYYKALVEAYEKAHPDVTVELVDLGSSDYSTVLT
                     TQLTGNGADFDVVSIKDVPGYMALVNKGVLEPLDPYIEKDGVDLSGYKGLTDQVKVDG
                     KMYELPFKSDFWVVYYNKDVFDNAGVEYPTNDMTLEQYDEIAKKLTNTEPGQEVYGSH
                     YHVWRSTVQMFGMIDGEHTILDGNYDYLKPYYDIILDEQENGVCQDYATLKTSNLHYS
                     GAFSQGNVGMMNMGTWFISTLIDKVKSGEYIDCANWGIVKYPHPEGVEAGSTAAQITS
                     LSIPTSAPNKEAAWDFMKFVCGEEGAAVLASTGNFPAIMTEETMNTIASTEGFPEDEN
                     SKAALETVNLYLEMPVDVKSPEVETVLNEAHDNIMAGNMTVEEGIADMNERVAAVLGE
                     "
     gene            10974..12833
                     /locus_tag="ACZPCG_03495"
     CDS             10974..12833
                     /locus_tag="ACZPCG_03495"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007715090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1284170.1"
                     /translation="MLQSVFPKTIRGKIILYTAAVTCSIAVFTVTICSFVFQSFLQKN
                     QIQTSEYSLEVICNSTAADLENILSFYKWCSFNTDIAAYLDGFENLSQMPSISSKNSG
                     LRSVALRTYDRLKEEYYNTHSSSYINRVIISPLNSCNYMQISDTTAVSSAHSLSDLTG
                     TDWFQTILSEDGMDNSTFMEDPFLPGSGLMVLPAVSALSGRYSSHQAGWIYMSISDRL
                     ILDYLKSFPLNPDSFLCLTIGGRTWLYENDRLKSCDWKFEPVKDLSENAFNSDTSVSL
                     CRLPDGSERVLLSCPIGNYGWSISLLLSQSAMKAQSTLYAVILSGIAVFILFIGLLLF
                     VLLNRSIGRPVKKLQHRIQAIAGGDFSLDPSIQREDEFGAIGTGINRMSQDLAALIQK
                     RIQDEKERKDLEYRILQSQINPHFLYNTLNSIKWMATIQGASGISEMTTALARLLKSI
                     SKGSHNRITLREELNLVKDYFLIQQYRYGGSISLECEISEEALYDCLIHRFTLQPLIE
                     NALFHGIEPKGCAGKIRISANLDREAARQPRLIISVWDNGIGMDAETIKRVLSGTQKP
                     TADFFRQVGINNVNQRIRYDCGPEYGISIESVPGEYTCMHITLPYETAQGEIL"
     gene            12830..14458
                     /locus_tag="ACZPCG_03500"
     CDS             12830..14458
                     /locus_tag="ACZPCG_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002600044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR1284171.1"
                     /translation="MIKLLIVDDEPLVQIGIKSMLRWDELGIEVCGTAMNGKAALEMI
                     RQYRPDIVITDIKMPVMNGLELVKICRDEFGPIPLFIILTSFEEFSLIKEAMSYQAVD
                     YLIKLELDPGSLKSSIEKASERLEELKAKESFRQNQGRPTLLSYHDKFFMRLLHNLFE
                     NRDQFSIQSRDLGLDFSARQYLACHGELLSCVSESLDPEGQINLYSSSLQMMREILTK
                     YAGCYAIALDKTRFALIFHFPDEQPEMETVLEALNNGRSMIRNYFNVELSAGIGEPVD
                     DPLKISISYQEARQAFNLCSPDAPFVPFTQTASSSIRDSFNLAVFKEDLTRAFEEFDT
                     DALYDTLTVISQLFAANPQKLLQTMDGACSILYLALSLLPEGEEILSGIFSASPDGYR
                     SIYRMKDVFQIVRWLEQLRDGLCEALKTRRKTYKDHVILNVQKYILNHIEEHLSLNEI
                     AAVFGLSPNYLSTLFKKTCSMGFSEYITQRKISRAKPLLLEQDLKIYEVADRLGFESA
                     FYFSKVFKKVEGISPREFVQAHMNEPNTHTILEE"
     gene            14463..16280
                     /locus_tag="ACZPCG_03505"
     CDS             14463..16280
                     /locus_tag="ACZPCG_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002600045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III domain-containing protein"
                     /protein_id="HIR1284172.1"
                     /translation="MFYEIAASPKLPLTPFIPFSPARCREFWEALPSSLKNRAVEDAK
                     ALCPVVWPQILACDFLEYTRSGNRIRFEDKQFARRTALGTLVLAEACDYKGTFLDDII
                     NGIYLICEETAWQLPPHNSYIRDTPSLPLPDPSRPVIDLFAAETGAVLAVAVWLLRDE
                     LRAVSPLIEKQVVRCLKERILEPYLKEHFWWMGDGVSPMNNWTIWCTQNVLMTAALWE
                     EDEEISRAILQKAAKSADFFLAEYGDDGCCDEGPQYYRHAGLCLFNTIEIMNGMTDHS
                     FSSLYREPKICNIAAYLSNVHACGPYYINFSDCAAVAGLCSAREYLFGKRTEQKELME
                     LAAGDCRITQDPLLTQEHNLFYRIQNLFALPEILSIPQGCNTEKKDIFYPSTGLFIAR
                     DSRFCLAVKAGDNDDSHNHNDTGSFTIYKDGKPLFADIGVETYQAKTFSPQRYEIWTM
                     QSAYHNLPSFITADGHEQMEHNGPDFKAEHVDWGFEAQEAHISMELKNAYPKGCITSF
                     KRSVVFKRSQEILITDQVCCPGLTPVLNLITAEKPLYKESCLILGTLAVCKITGALTD
                     VSVECLPITDPRLKLSWKNDLWRTRITFNPKKELALRII"
     gene            complement(16302..19385)
                     /gene="lacZ"
                     /locus_tag="ACZPCG_03510"
                     /gene_synonym="bga2A"
     CDS             complement(16302..19385)
                     /gene="lacZ"
                     /locus_tag="ACZPCG_03510"
                     /gene_synonym="bga2A"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005990 - lactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase, LacZ type"
                     /protein_id="HIR1284173.1"
                     /translation="MKSRNPRISWLDNPEVFEVNRNNAHSDHVYPFFQEAGSEGIGFM
                     LLDGVWDFCWEEQPPEYALRGTEDSDGVAFYKAAFEEKDWGTIQVPGHVQLQGYDNCH
                     YVNTMYPWDGRSEIRPPAIDWENAPAGSYVREFIITDQEKDFLEKRVFVSFQGVETAF
                     YVWCNGQFAGYGEDGFTPSEYELTGVLHPGVNRLEVRVYKRSSASWIEDQDFFRFTGI
                     FRSVYLYCIPEIHVEDLFIKAYLTDDYRDGFLDVEFTMENPNGYSYEIRLALEDAGRI
                     IKTGALKGRMEKSCAVSLGKIPNVHPWSAEDPFRYLLHIELWGEDGCKESFLQPVGFR
                     SFELKNGQMCLNGCPIVFHGINRHEFDWHRGRAVTADDMLYDIRFLKQHNINAVRTCH
                     YPNQSLWYELCDRYGIYVMDEANLESHGSWQKMGACEPSWNVPGSLPQWKACVTDRGK
                     SMAERDKNHPSVLIWSCGNESYAGEDIRAMSRWFKERDPGRLIHYEGVFWNREFEDIS
                     DMESQMYTPPERVREFLENNTKKPFIMCEYMHAMGNSLGGMDRYMALEYEYPAYQGGF
                     IWDMIDQAVARQGKDGKEYPAYGGDFGDRPTDWQFCGNGILYPDRSISPKAAEVKAQY
                     AAVRLKISPERRQIQIENRQLFSDLSDLAFVLTVLRNGKKWKTFPFKAQAAPGTCAVY
                     SFDETGFTNDTALEDGKEYILRVSVHLEKDTLWAEKGYELAFSEAVLPGNWKMERKRF
                     IPARMIDGDVNLGVILGEMQLMFSYQDGGLVSLKRKDGIQWMIRPPVPVYWRASTDND
                     RGNGFAKESRLWLAKTLFPEICKGKAEIRETDKEAELTFTWEDTTVTYRMRKDGMLGV
                     RAHFHGKKGYPSLPVFGLAFELPEQVSRFSWYGRGPWENYPDRRAGAGLGVYESTKKE
                     QLARYLKPQECGMHMDTRRLVLETKDGEKLLFEAEKKPFHFSLLPYSPIELETALHRE
                     ELPAQSHTWAVIAGAIRGVGGNDSWGAPVYPEYEIPGDRDIIFEFSMGFISNHEE"
     gene            complement(19375..21192)
                     /gene="uidA"
                     /locus_tag="ACZPCG_03515"
     CDS             complement(19375..21192)
                     /gene="uidA"
                     /locus_tag="ACZPCG_03515"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012740861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HIR1284174.1"
                     /translation="MLYPKMNRARTVMDLSGLWEFKLGDYETPGDNCERLAGGERIAV
                     PASYNDQKDDPAYKNHYGWAWYEREVTVPAFFKGQRLFLRFDAVTHRARIYINGTFVM
                     EHKGGFLPFELELTELVVPGETLLLTVAADNRISHSTLPVGNEGGTAFFGSDNPGVPS
                     VEAAKVWRKQGNLPNFDFFNYAGINRPVRLYTTPVSRICGLTLIPDIDDTDGILQYEA
                     ETEGEGAVHVEIFDVEGNKVAEAEGASGTITIPDARLWWPWPGTPYLYRAVFTFGEDI
                     YEETFGIRTVKVEGTQFLINGKPFYFKGFGKHEDSPFHGRGLDLCLDVKDVNLIHWLH
                     ANSFRTSHYPYAEEMYQLCDKGGIVIIDETPAVGIGAGEHCDPYQTFPIREHHEAVLR
                     DMIERDKNHPCVVMWSMGNEPDTEHFPESAYEYWHSLYEYTHTLDPQDRPVTFVCCQN
                     NYEKDLVTRTMDVVCINRYYGWYNLSGDLDAACYALNMELDFWEKIGKPVMITEYGAD
                     AVAGIHQCVPEMFSEEYQMEYYRRQNEEFDKRSFFMGEQVWNFADFAAFQGCMRVDGN
                     KKGLFTRERRPKLAAHYFRKRWENIPNFEYKAGGNTDEK"
     gene            complement(21337..22122)
                     /locus_tag="ACZPCG_03520"
     CDS             complement(21337..22122)
                     /locus_tag="ACZPCG_03520"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HIR1284175.1"
                     /translation="MEQQKSVIIDAGHGGTEPGAMFEGRKEKDDTLRLAVAVGEILEN
                     NGIRVMYTRTTDVYDTPLEKAMIANGSGADFFVSIHRNAMPVPGTGKGALNLVYENTG
                     PGALMAENIQKNLVKAGFDDLGVQERPGIIVLRRTKMPAVLVEAGFLDHPEDNRIFDE
                     QFDQIAQGIADGILTTFSQLKKETEEKRYYQVQVGAYRERPRAEFLVRELAARGFPAF
                     LVYDDGLYKVRVGAFLQMDNGVRMEQRVRQAGYPTMLVREEEV"
     gene            complement(22346..23185)
                     /gene="dapF"
                     /locus_tag="ACZPCG_03525"
     CDS             complement(22346..23185)
                     /gene="dapF"
                     /locus_tag="ACZPCG_03525"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006773059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HIR1284176.1"
                     /translation="MNFVKMQGIGNDYVYVDCFKENVKNPSAAARFISDRRFAIGSDG
                     LILICPSDVADCRMEMYNTDGSQGKMCGNGIRCVGKYAYDHGIISPDRRSLTVETPAG
                     IKELKLEVENGRAVMLTVDMGEAVLTSQLPETILVDGKQWEFTGIDVGNPHAVYFMDE
                     IDSLDLEKMGPSFEFHERFVPDRVNSEFIRVIDRTHIQMRVWERGSGETWACGTGATA
                     SAMAAILLGLTEDEVLVSLKGGDLRIRLDRESGHLFMTGPAVEVFNGAIEPFWEDGKE
                     GSL"
     gene            complement(23195..23743)
                     /locus_tag="ACZPCG_03530"
     CDS             complement(23195..23743)
                     /locus_tag="ACZPCG_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing response regulator"
                     /protein_id="HIR1284177.1"
                     /translation="MASIIAAFSKPQDAGNIRAILRKNGYEPVFACTSGAQVLAAAES
                     LNGGIVVCGFRFEDMVCEELRRLLPSSFDMLLIASPAKWSKRDTEGMICLSMPLKVHE
                     LLSTIEMMLEARKRRKRRKRTRPQERSAEEKELILKAKALLMDRNTMTEPEAYRYIQK
                     CSMDNGTSLTETAQMIISLIHM"
     gene            complement(23774..>24289)
                     /locus_tag="ACZPCG_03535"
     CDS             complement(23774..>24289)
                     /locus_tag="ACZPCG_03535"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003524501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HIR1284178.1"
                     /translation="HFMAGILNHMKEMTLLTNPTVNSYKRLVPGYDAPVYIAWSPSNS
                     KSCLIRIPSPGGEGIELRCPDGAMNPYLALAACLQAGLEGIEKELELPLCAKEPISLA
                     DQEELNNCGIDRIPNTLGAAIEAYAGSLFVKNVLGEPIYIKYLEAKEKEWGEFGIQVT
                     DWEIAQYLYQY"
BASE COUNT         6178 a         6527 c         4911 g         6673 t
ORIGIN      
        1 gtggaatatt gttaaaaata atactggtaa ttgactgaag actggaaaaa ataagcctta
       61 cgatttcatt tcccaaaaaa ggaaatttat cgatcatata agactgtccc atagcactgc
      121 agatacccat taccataccg atcggtgcca gcagagcaat cggaagaagc agggaacgtc
      181 caaaggtctg tattcccgct ttccagcttg aatttttctt gttcataccg ttctcctcca
      241 tagatgtgat ttcatttgca taatacagac gcaggcctta catctgcctt ttttcaccca
      301 tagcgcgctt gtgaactgtg tccattatgc cagaggaatg cggaagcttc aaggcagaaa
      361 acgtatagaa atttcttgca tttagttgcc tttattttta taatatattt atctgattca
      421 gtatataaaa agaggtatct atatgggctt taattacaat gatcttacac tccttcgctt
      481 tatacaaaag cgccactcca ttccccttgc caaggctacc cttcaatttg gaaaaaatga
      541 aacctcaatc cggcgggcag tagaacaaat caacctttcc tttccctctc cagtcattga
      601 aatcgaaaag ggaatctgct ccagccgttt aagctacgaa gagcttgtat ctctcatccg
      661 cagtatgagt ccctctgact acgccagctc atccagtgag cgaattatgg ttatgattgt
      721 cactattttc ttctgcggct acgtaaatgc ctcggaactg tatgaaaagt ggggcttgtc
      781 tctgacaaca aaaaaacagg atacagggca gctgcgatct tttctgaaaa aatacagact
      841 gtttctgaca accctccaca aaaagggact taccataacc ggagacgagc ttcaactctg
      901 ttttcttgta atcgatattc tccacccatt gctggaattc acaccggaaa accgggttca
      961 tgcccgtttt gccaatactc ctattgaacg tcaaagtttc gaaacggcta tgatatatct
     1021 ggaaaaagga ttttcccctg ccgctgaaaa actgactcat tttcttgaag aaaaccacct
     1081 ggcattgaat tatccatcta aaaaattcct tctgcttttt atctgtatca tggaaacccg
     1141 tcccttaaaa ccggacacaa cattttcctg ccgtctgcct tttaaacctc tggatctcca
     1201 tttcacagac tcacccatgg aaaataagct ctataatgta gctttaagta tgcagaactt
     1261 ttcaaaaaat cccacacttc cctttgatgc ccgcctttgg cagattacag agcgttttgc
     1321 agaagatgtt gtaaacggat tggcacatcc tttttccatc agcgaggatt ttctgacaga
     1381 actttacgaa tatttttaca gagaaatcac cctggactat tttcattgta cgtttgtaga
     1441 taaaacagta gaaaatacag ccggaaaatt tcctgtactc tacgaacaga tccgcagata
     1501 cggaatatat tttcaggctg catataattt ttcccttctg gatgagcatt tgtccaccct
     1561 gacccttatg gtggaaaaac atattataaa aaaccggact gccgaccgca gaagaaagcg
     1621 aatcattatt atgaccagca ttaactttga acgaatcagt ttttttctgg agcagttaag
     1681 agaatacatt gcatttcagt gggtagaaac cctgaacctg aacgaaatac accgattaaa
     1741 tgatctgtca tatgactgta ttttctgctt ttctacccga attttcaata ttctcaacag
     1801 tcgggaactt ccggtaatca gggtgaactt ttttctggaa aacaaagata ttgaccggct
     1861 gcttaagctg ggattttctg cccagacaca tcgttttaac gccaattctc tcgctcttga
     1921 tctggccgga aaaacagagg cggaaatggt atcctatctg aaaaacagat ttggagatta
     1981 ttttgtatga tctggctcac tgcccctata taagctccgc catccttgta atccccacat
     2041 agatatggga aatccggtac caggtagcaa aatccaccgc tcctgtctgg ggaaggccga
     2101 agatgctctg gaattcccgc accgcagccg ctgtgcgcgg gccgtagatg ccgtcgggcg
     2161 ctactctggg aatcgctgta taaacagtgg ctatggcgtc cagctgttcc tgaagctgac
     2221 gcaccttgga gccggagctt ccctggctta gatcagaacc gggccatgag gcaggaatgc
     2281 ctgaaatctg ctcggcagta ttgatataaa tagagtctcc atagaaatac cgtaaaatct
     2341 ctataggact gtagccctgc tttcccagat cacaggatcc ccattgcgtc atccagcctt
     2401 cacagctgac ctgccttccg tcgcagtact gggtcagaat cggctgacgg acgccgggac
     2461 gggacagata ggcgtcaaaa atttcatcca ctacctgaga aatgctctgg aaaatatttc
     2521 tgccgtaaat ccatttatgg tcaaaagctg tagatgaggt gatggtaaaa tcatagccct
     2581 gatttctgta ccattcagtg tacacacggt ttaaggtaaa ggacatgatc gccagtacat
     2641 tagcggtaat ggagctttcc ggccaggtag agtaaatttc actgcaggct acatttttaa
     2701 tatagtcccg atagggaacg taatagtttt tagcggtaga gtcagtggga acgccatcat
     2761 gaaccactat ggtctgggga attaccacac ggcttaatac gatttctccg ctttcattga
     2821 ccggtttgac ctcatcctcc gcaatcttgg gcggataatt accgtaaagg gtatgatccg
     2881 gaatcactac cggatcttcg ggaggctgct cctgcgccaa gggtgtcata gccgcaggct
     2941 ggatcgcatt ggaatctgct aaaatctctg tgccggaaat cgccagaggc tcaaatccct
     3001 ctgcgcggac gatgagatcg tactctgaat agggctggat gattcctgga ttcaggctgt
     3061 attccaccgg aggcgcagga agggagagct gttctgtctg tccggaggaa ttggtgctta
     3121 cttcctccat cacccgatcc ggctctccct ttgtgctgat acttatagtt gcatttttaa
     3181 ttggaaagtt gttctgaata ttgactacat tgacctgtaa gaaacctctg gaatttgttt
     3241 cctgagcttt taccatttga aacctcaaaa aatccttgct tacttacaat ataagcaaat
     3301 ttttcctgat tatgacttca aataagaaaa accgctctgt tttttctgtc ctgtcctcat
     3361 ttcaactcca agattccttc ttcccagatg acgacatcca ttaaaagatc atgggcttcc
     3421 caggggattt ccgcctccat cagacgccct ctgcaaagca cggcccaagg gccgggaacg
     3481 gatcgcagaa acctatcgta aaaaccgcct ccatatccca gacgccggcc gtcctcagag
     3541 caggtcagac acggaacaag tgccagcgaa atttcttccg gccctatgat ctcggcagct
     3601 ttctccggct cctcaatgcc atagcttccg ggatacaatt cgccaagaga acggatctgc
     3661 cgtgcctcca tgatcccctt tgaaatgcat tttggcacag ccacccgctt tccctgatcc
     3721 aaagcagcct gaatcagcgg tttggtatga atctccccgg cagttcccac aaagcagaat
     3781 atcgtttctg ccttttgaaa atatttccag tttaaaatcc attcccggat acgcttatct
     3841 gcccaaaggc agtactccgg atccagatga gcgttttttt ctctgatctg tttgcgcaga
     3901 atctgttttt tggtatttat cttctgagtt tccattggct gaaacccctt tttattttta
     3961 cgtgaatgta tgccggaagc cccctcgtta taacaggatc tcctttatcc atttatttag
     4021 ttattatatg gaagaaatcc agatgtgtca attcatccgc ctttttagta acaaagaacc
     4081 gttcccccca cgcttccctc tgctttatca aagacaatta catttccata tcccatgcag
     4141 cccgctgcct ccgtaagatc tgtgggggaa ttaacctcct gatggcagag gatgactaca
     4201 agcgttctgc ttccgtcaac aatctccaca gcctcagcca tagacggcgg ataggtgatt
     4261 cccttaagaa cagacgttac cggaaccagg gatgcgtgaa gctgtctttc ctttcccttc
     4321 tttccgcctc cgattacagt aatcatggac tgaaagccct gtcctttctt ttgcagacag
     4381 aggcggtctc tggtctcata ccggttataa tggcgagcga tctttccctg cttcatttct
     4441 ccttcgcagt ccgggctgat aaagagaagc tttacctgtg ccttttctcc tgtgaagcag
     4501 gcacagccat tttcataaac tgcctttcct gtctcactga aattccagaa ctgctcaaag
     4561 gtatgttttc cctgtccata gagctcgtct gctatcacaa aaaggccgtc ttttaaagaa
     4621 atcactttgc ggtttacaaa tacgcccttg tgcatatatc ccagatttcc gccctgaacc
     4681 aggctgtatc cgtttttctg cttatactgc tgcttgaccg gctgagaaag cttagagcat
     4741 tcccaggaat ccttacactg tgtaaaaaat tcgccatcta ccgtaactgt attgtgcgca
     4801 tccggatctt taaattcaaa tcttccccga tccatcacat aggtataacg gccggaatcg
     4861 gtcaggatat cctcgcctcc cagaataaga tccacatgaa gcttatcaga atgtccatgg
     4921 cctgctccca tggttccgca gtgaaaatgg agcagatctc cgtcctcatt ccaggaactc
     4981 cggatataat aatttccgct gtcctccaaa gccgctgata caaaatccgg tttccggcat
     5041 tccatacggc tgtattccgc tgcgccgttc acccctacat cccagagact ttcaaaatcc
     5101 agctccttaa aacctccgaa tttcagtacc ggatctttta acaggtaggc tgcaatactc
     5161 atataatctc ttaaatccat atcatcactg tcacccatga tgatctggcg gtgatccggc
     5221 ctcatccatg ccagatttgc ataagccatc cttcttacgg cgtcgcagat ccgttctgaa
     5281 acagcaatcc cattccgttt tgccaaaagg agcacatcct catagcagtg aaaaacctcg
     5341 ttgtgataca taggagattg ctcccactgt acgccgtcgt caaaaatctg cattgctgcc
     5401 tctgtttcca gacgttccaa tgcaatagag gtaaaacgat cccttgtttc ctgatccggc
     5461 atctggattc cgatctcaaa caggccgtgg ttttccatca ctccccagtt actgatatag
     5521 cggtagggcg aatgcatctg tataatatac tccccgtgct ccctgagaca gcattcaatc
     5581 ttttccgccc tttcatccgt cattaaagga ctgtctttaa aataccgcag cgccttaacc
     5641 cagttttcgc cccggatccc cacctctaaa atccgccatg tagtctgctc tgtttccggt
     5701 gtgcgcttta catggtcaat gaaatccata aggagacgga aaaaatgatg ctcatatttt
     5761 gagtctcctg taagccagta cgcctgtccc agacagatca gaaaacggtg cctgttaaac
     5821 tgaaaggtaa attccgggtc atttcccggg cggtactccc aatcaatggg gcgatcctcc
     5881 ggaaatgtca ctggctccca ggtacgctct aagtcatgat ccagatcaaa cagaaaataa
     5941 ttcctgcaga cctcatctgc cacccgtata atatgggcgc agtcctccgg atatctttcc
     6001 cggcagactg cagctgtttt ttcaggatca tcaaagaaaa agtattcatt tcctctctca
     6061 aaaagttcct ttgctgtcat ccttattcct ttccggccgt ttcatgccgt atatgatatt
     6121 ttaccagtat acgttccagt ccttatacat ccttgtcagc gcttccatat agaagtaatc
     6181 accccacagg ttgcactcat ccactccata gtggttacag gtattgtaag gcgagtgatt
     6241 agaataggtg ctgtgaagca ctaggccgtt ggagctgtca aaatccttta ctgcatactt
     6301 ttccgttaag gacttcataa atctggctgc cagagagcgg cacagagcgc ccttttccgg
     6361 ctctacatac tttgccattt caagcaggcc gcaggctgca atcgaagcag aggaggaatc
     6421 tcttggctgg tcatccccat caccaaattc cagatcccaa aagggaacca gatcctcagg
     6481 aagatgctcc agatagtaat tcgtcacgcc ctcaaaaacg ggtatatagc tctccctttt
     6541 cgtatattta tagccaatag ccgtcccgta gatcccccat gcctgccctc tggcccaggc
     6601 ggatccgtcc cggtatccct gacaggtagc gccgtgatcc ggctctcctg tttcacggtt
     6661 aaagaaaaac gtatgccagg tagaaaaatc cggacggatt acattggaaa gggccgtatg
     6721 gatgtgacgc tccgcaatct ccttatatct gggatctccc gtttcctgag aagcccagta
     6781 aagaagcggc agatttaaaa ggcagtcaat gatcagcctg taattatccg gagcatcaag
     6841 aggcccccat gcctgaataa actggccctt ttcgtgaaag cgtgtaatca gctcctcagc
     6901 tgctttcaaa gcggcttccc gtcccactct gcttcccgta agcttgtagc ctgccacgca
     6961 ggaaggggaa tacataaagc ccagatcatg tgtttccaca tcctgttttt cattgatccg
     7021 ctttaaaaag ctcgccatct ggagctcgcc cgcctttttt aaagcctcat tgcccgtatg
     7081 ttcataggca agccatattt ccccggtcca aaatcccggc gtccagtcca cattttcagt
     7141 aggcgcataa aaaccgtttt cgctgtacgc cttctggaaa tggtctgtaa actgatccag
     7201 atttcttatt acctgactgc aggccgcatc cagagcccgg cctgcctctt cttttgtaag
     7261 ctccggcatt ttttccagag catcccatga tatccagctc ataatctgtt tccttcctgc
     7321 tttatgcatt attttaatcc cgtatcatcc ctttacgccg gaagctgcta cgccctcaac
     7381 aaaatatttc tgcagagaca ggaatactat cagcacaggg atcagactca gcacagaagc
     7441 agccatgatc agaccgtatt cagaaccaaa ctggctgata aacatacgca gtcccagctg
     7501 cagggtcttt tttgactctg tcgtcagata gatcaatggt cccaggaaat cattccatgt
     7561 atttacaaag gtaaagatcg tcagggttga cagagcaggc tttgcaagtg gaagcatcac
     7621 ccggtaccag atccgatact cgctcatgcc gtcaattctg gctgcctcgc aaagttctgt
     7681 gggaatccct tcataaaact gcttcatcat aaatacgcca aaggcggaaa atgcctgcag
     7741 acagataatt gccagatgcg tatctgccag tcccatggaa cgcatcatca taaactgcgg
     7801 aaccatgtaa acctgccagg gaacagcgat agttgcaata tacgccagaa ataaaacgtg
     7861 ctttccctta aaattcagct ttgaaaaggc atatgccgca aagctacttg taagaagctg
     7921 taaaaaggtt acgatcagcg tcagcttcac cgtattaagc acaaatttaa gcagcgggat
     7981 cctggtccag atatctacat aattctgcca tctgggatta gttggaatcc actggatcgg
     8041 gaaaataaat acgtccttat ccagcttaaa ggaagctgaa agcatccagg caaaaggtac
     8101 cagcatcaga aatgccagaa caataagcac aatatagaga gccgccctca tcatccttgt
     8161 ttttgtattt tttatcttca tttgaggcct cctcctttaa tttgcatatt tcttttcccc
     8221 acggaactgt accagggtaa tggcaagaac aataaggaac agtaccatgg ctacagcgct
     8281 tgcgtaaccc agattccagt tgcggaaggc ttcctcgtaa atgtggtata ccaggacaat
     8341 ggcagactca tttaatacgc ccgtgcttcc tcctgccagc atatatacaa tgtcatatac
     8401 tttaaagcag ttgatggtaa ggataatggt aacaaaaaag gtggtcggct gaagctgggg
     8461 ccaggtgata tagcggaatc tctgccatgt attggctcca tccaagctgc atgcttcgta
     8521 aagctctccg ttgatcccct gtaagcctgc cagatagatc accatgtagt atcccatatt
     8581 tttccataca gagaacagga ttacggtgaa cataacccag tccttgtctg cagaccattt
     8641 tggcagctcc ttcgccgcaa cgcccagatt ataaaggatc atatttacag gaccgctttt
     8701 ggcaggggaa aacagcatat tccacacagc tgctactgca accagagaag ctacataggg
     8761 gaaaaagctt accgtacgga agaagttgcg tccttttacc ttctgattta aaagaactgc
     8821 cagcccaagg gctactgcca gagtaaaggg aaccgtagcc acgcagtata caactgtatt
     8881 tttaagggaa gcaaggaaac gcttgtttcc caccatctgc ataaaattgt ccaggcccac
     8941 aaaatccatc gggttattgc catcccattt gaaaaatgcc agtataaaag caaacagcac
     9001 agggcccaga gtaaaaacgg caaagccgat aaagttagga gcgataaagg agtacgcgat
     9061 caaatcccgc tttgtctgac ggctgaaccg ctctcctgtc tttgcctgac gtattttatc
     9121 acggatctga tccgcctttt ttgccagctt tacctttttt ttcatatccg tttcttcatc
     9181 tgcctgcttc aaaagagctg caagccgcgg ctccagaata tccagttttt ccctgaccct
     9241 tgcaggatca actgcttttg tctttcccat attcatctac ctttctttaa atgaggcagg
     9301 cggtaaatcc cgtctgcctc gttttcagct ctgtctgtcc gtatatgttt ttcttattca
     9361 cccagaactg cagctacacg ttcattcata tctgcaattc cttcttctac ggtcatgttg
     9421 cctgccatga tattgtcatg agcctcattt aatacagttt ctacctcagg gctctttaca
     9481 tcaaccggca tctccagata caggttcact gtttcaagag cagccttact gttttcatct
     9541 tccgggaacc cttctgtgga tgcgatcgta ttcatggttt cctctgtcat aatcgccggg
     9601 aagtttccgg tagaagctaa aactgccgct ccctcttcac cgcagacaaa cttcatgaaa
     9661 tcccatgcag cttccttatt cggtgcgctt gtaggaatgc ttaaagaagt gatctgggca
     9721 gcggtagaac ctgcctctac gccttccgga tgagggtact ttacaatacc ccagtttgca
     9781 cagtctatat actcgccgga ttttacctta tcgatcagag tagagataaa ccaggtaccc
     9841 atattcatca tacctacatt accctgggag aatgcgcctg aataatgaag atttgaggtc
     9901 tttaaggttg cataatcctg acatacgcca ttttcctgct cgtctaaaat aatatcatag
     9961 tatggcttta aataatcata attgccgtcc aggatcgtgt gctctccgtc aatcatgcca
    10021 aacatctgaa ccgtacttct ccaaacatga tagtgagatc catatacctc ctgtcccggc
    10081 tctgtatttg taagcttctt tgcaatctca tcatactgct ccagggtcat atcattggtg
    10141 ggatactcta cccctgcatt gtcaaataca tccttgttgt aatatacaac ccaaaaatca
    10201 ctcttaaatg gcagctcata catctttccg tctactttta cctgatcggt caaccccttg
    10261 tagccgctga gatccacgcc atccttttca atataaggat ccagaggctc taacactccc
    10321 ttatttacca gagccatata tcccggtaca tccttaatgg aaaccacatc gaaatctgct
    10381 ccgtttccgg tcagctgtgt cgtaagaacc gtactgtaat cagagcttcc cagatctacc
    10441 agttctaccg tcacatccgg gtgcgccttt tcataggctt ctaccagagc tttgtagtat
    10501 acggtcaggt ctttatccca catagcccat tttaaatgtg tttttccgtc tgctgccgcc
    10561 tctgtctcct ctgcctttgc tgcggttgta tccgctgctg cctctgtctc cttgttctct
    10621 gtctttgctg ctccgccccc gcaggctgta aggctacccg ccatggaaac tgccaacgct
    10681 aaacttacaa tacgtttttt catcatcttc tcctccttga tccatcatat tttttgttta
    10741 tcctgatgtt gtgcctttat tatacaggca gattccctaa gactgaatgt aaaaacaacc
    10801 aaaaaaactg tgattttttt ctgtaatttt tctcttttcc attcttcccg taaaaaagct
    10861 gtacttttct atgatttgca gtcttttctt ataattgtat tcatttgaca aaaaatagct
    10921 ttcctattat agtattttat ggataataca taccagaggg gggactattt ttcatgctgc
    10981 aatccgtatt tccaaaaacc atacgcggaa aaatcatact ctatacggct gccgtaacct
    11041 gctccattgc tgtatttacg gtaaccatct gttcctttgt ttttcagtcc tttctccaga
    11101 aaaatcagat acagacttcc gaatacagtc ttgaggttat ctgtaacagt acagcggccg
    11161 atttggaaaa tatcctttcc ttttataaat ggtgctcctt taacacagat atcgccgcct
    11221 atctggacgg ttttgaaaat ctgtcccaga tgccctcgat ctcttccaaa aattccggcc
    11281 tacgcagtgt ggcacttcgt acctatgacc gtctgaagga agagtattac aatactcatt
    11341 cttcctccta cataaaccgg gttatcatat ctcccttaaa cagctgcaat tatatgcaga
    11401 tttctgatac caccgctgtg tcttccgcac attctttatc tgaccttaca ggcacggact
    11461 ggtttcagac catcctttca gaagatggga tggacaacag cacctttatg gaggatccat
    11521 ttcttcccgg aagcggttta atggtccttc cagctgtctc cgccttgtcc ggacgctaca
    11581 gctcccatca ggccggctgg atctatatgt cgatctcaga ccggctgatc cttgactact
    11641 taaaatcttt ccccttaaat ccggattcat ttctctgcct taccatcgga ggcagaacct
    11701 ggctttatga aaatgaccgt ttaaagtcct gtgactggaa atttgagccg gtaaaggact
    11761 taagtgaaaa tgcctttaac agtgatacct ccgtttcgct ctgccgtctt ccggatggat
    11821 ccgaacgcgt actgctctcc tgccccattg gaaattatgg ctggagcatc tctctgcttt
    11881 tatcccagag cgctatgaag gctcagagca ccctttatgc ggtcattctt tccggtattg
    11941 ctgtcttcat ccttttcatc ggtctcttgc tttttgtact gttaaaccgt tccatcggac
    12001 gtcctgtaaa aaaactgcag catcggatcc aggccattgc tggaggcgat ttttctctgg
    12061 atccctccat acagcgggag gatgaatttg gagccattgg taccggtatc aaccgtatgt
    12121 cacaggatct ggctgctctg atccagaagc ggatacagga cgaaaaggaa cgcaaggatc
    12181 tggaataccg gattcttcaa agccagatta acccccattt tctttacaat acattaaact
    12241 ccatcaaatg gatggctacc atccagggcg catccggtat cagcgagatg actacggctc
    12301 tggcccggct attaaaaagc atttccaagg gttcccataa taggattacc ttgagggaag
    12361 agctgaatct ggtgaaggac tattttctga tccagcagta ccgttacggc ggcagtatct
    12421 ctctggaatg tgaaatttcg gaagaagccc tctatgactg cctgatccac cgttttacct
    12481 tgcagcctct catagaaaat gctcttttcc acggaattga accaaagggc tgtgcaggaa
    12541 aaatccgcat cagcgctaat ctggacagag aagctgccag acagccccgt ctgatcatat
    12601 cggtatggga taacgggatc ggcatggatg cggaaaccat aaaacgagtt ctttccggca
    12661 cccaaaagcc cactgcggac tttttccggc aggtgggaat caacaatgta aaccagcgga
    12721 tccgctacga ctgcggcccg gaatacggta tttccattga aagcgtaccg ggagaataca
    12781 catgtatgca tattacgctg ccatacgaaa cggcacaggg agagatatta tgataaaatt
    12841 actgattgtt gacgatgaac ctctggttca gatcggaata aaatccatgc tccgctggga
    12901 tgagcttggc atagaggtct gcggcactgc catgaacgga aaagccgctc tggaaatgat
    12961 ccggcagtac cggcctgata tcgtgattac cgatatcaaa atgcctgtta tgaacggact
    13021 ggagctggtc aaaatatgca gagatgagtt tggccccatc cctctattca tcatccttac
    13081 aagctttgaa gaattttctt tgatcaaaga agctatgagc tatcaggcag tcgactatct
    13141 gatcaagctg gagctggatc ccggatcgct taaatcgtcc attgaaaagg catccgaacg
    13201 tctggaggag ctcaaagcca aggaatcctt ccgtcaaaat caggggcgcc ctactctttt
    13261 aagctaccat gataaatttt tcatgcgtct tcttcataac ctttttgaaa acagggatca
    13321 gttttccatt cagtccaggg atctgggact ggatttttct gccagacagt atctggcctg
    13381 tcacggcgag ctgctaagct gtgtttccga gtctctggat ccggaaggcc agataaacct
    13441 ttattccagc agcctgcaga tgatgcggga aatccttaca aaatatgcgg gctgttatgc
    13501 gatcgccctg gataaaacac gttttgccct gatctttcat ttccccgatg aacagccgga
    13561 aatggaaacc gtgctggaag cgctcaataa cggccgttcc atgatccgta attattttaa
    13621 tgttgaacta agcgccggga tcggagaacc ggtggatgat cccttaaaga tcagcatttc
    13681 ttatcaggag gccagacagg cctttaatct ctgctctcct gacgccccat ttgtaccttt
    13741 tacccagact gcctcttctt ccataagaga ttcctttaat ctggctgttt ttaaagaaga
    13801 tctgaccagg gcctttgaag agtttgatac agacgccctg tatgacaccc tgaccgtgat
    13861 ctctcagctc tttgcagcga atcctcaaaa gctgctccag actatggacg gagcctgcag
    13921 cattttatat ctggctctct ctcttcttcc ggaaggtgag gaaatactaa gcggcatctt
    13981 ttctgccagt cctgacggct accgctctat ttaccggatg aaggatgtgt tccagattgt
    14041 ccggtggtta gagcagctca gagatggact ctgcgaagcg ttaaaaaccc gccgaaagac
    14101 ctataaagac catgtgatct taaatgtgca gaaatatatt ttaaaccaca ttgaggagca
    14161 tctttctctt aatgaaatcg ccgcggtctt tgggctgagt cctaattacc tgagcactct
    14221 tttcaaaaag acctgctcca tgggtttttc cgaatatatc acccagcgca agatctcccg
    14281 ggccaaaccc ctgcttttag aacaggattt aaagatctat gaggtggctg accgcctcgg
    14341 ctttgaaagc gccttttact ttagcaaggt atttaaaaag gtagaaggaa tctctcccag
    14401 agagtttgtc caggctcata tgaacgaacc aaacacccac accattttgg aggaataaca
    14461 ctatgtttta tgaaattgcc gcttcaccca agctgccact tacccccttt atcccctttt
    14521 cccctgcacg ctgccgggaa ttttgggagg ctcttccttc ttcccttaaa aacagggccg
    14581 tggaagacgc aaaggccctc tgtccggtag tatggcctca aatcctggcc tgtgactttt
    14641 tagaatacac acgttctgga aacaggatcc ggtttgagga taagcagttt gcccgccgga
    14701 ctgccctcgg aaccctggtt ctggcagaag catgtgacta taagggaact tttctggatg
    14761 atatcataaa cggaatttat cttatctgcg aagaaactgc atggcagctt cccccccata
    14821 attcctacat ccgtgatacg ccttcccttc ctctgcctga tccctcacgt cctgtcatcg
    14881 atctttttgc tgctgaaaca ggggccgtgc tggctgtagc tgtatggctt ttgagggatg
    14941 aacttcgcgc cgtaagtccc ctgattgaaa aacaagtggt acgctgtctg aaggaacgca
    15001 tccttgagcc ttatttaaag gagcatttct ggtggatggg cgatggggta agccctatga
    15061 acaactggac tatctggtgt acccagaatg tgcttatgac agcagctctg tgggaagagg
    15121 atgaagaaat cagtcgggcc attcttcaaa aagcggccaa aagcgctgat tttttccttg
    15181 ccgaatacgg agacgacggc tgctgtgatg aagggcctca atattaccgt catgcaggcc
    15241 tctgcctctt taacaccata gaaatcatga acggtatgac ggatcattcc ttttcctccc
    15301 tttaccgcga acccaaaatc tgcaatatag ccgcctacct ctccaacgtc catgcctgcg
    15361 gaccctacta catcaatttt tcagactgtg ccgctgtcgc aggcctgtgc agtgccagag
    15421 aatacctctt cggaaagcgc accgaacaga aggaactgat ggaactggct gctggcgact
    15481 gccgcataac acaggatccc cttttaacgc aggagcataa tcttttttac cggattcaaa
    15541 atctttttgc actgcctgaa atcctttcta tccctcaggg ctgtaataca gaaaaaaaag
    15601 atattttcta tcccagtacc ggccttttta ttgcaagaga cagccgtttc tgtctggctg
    15661 taaaagccgg ggataatgat gacagccaca atcacaatga tacgggaagc ttcaccattt
    15721 ataaagacgg aaagccgctc tttgctgata tcggagtgga aacctatcag gccaaaactt
    15781 tctcaccaca gcgatatgaa atctggacca tgcagtctgc ttaccataac ctcccctcct
    15841 ttatcaccgc agacggccat gaacaaatgg aacacaacgg tccggatttt aaagcggaac
    15901 atgtagactg gggctttgaa gcacaggaag cccatatttc aatggagctg aaaaacgcat
    15961 accccaaggg ttgtatcacc tcctttaaac gcagcgttgt gtttaaacgc agccaggaaa
    16021 tccttattac cgatcaggtc tgctgtccgg ggcttactcc tgtcctgaac cttataaccg
    16081 cagaaaaacc tctgtataaa gaaagctgtc tgatcctggg aactctggcc gtctgcaaga
    16141 taacaggagc tttgacggat gtttctgtgg aatgtctccc cattacagat ccgcggctga
    16201 aactgagctg gaaaaatgac ctatggagaa cccgcatcac atttaacccc aaaaaagaac
    16261 tggcgcttcg gatcatatga tcctgagcgc cagctctttt tttattcctc atgattactg
    16321 ataaaaccca tagaaaactc aaatataata tccctgtctc ccggtatctc atattccgga
    16381 tataccggag ctccccagct gtcatttcct cccacgcctc ttatggctcc tgcgatcact
    16441 gcccaggtat ggctctgtgc gggcagctct tccctgtgga gagccgtttc gagctctatg
    16501 ggactgtatg gaagcaggct gaagtgaaac ggctttttct ccgcctcaaa caaaagtttc
    16561 tctccgtctt ttgtttccag aaccaacctc cgggtatcca tatgcatccc gcattcctga
    16621 ggctttaaat accgggcaag ctgctctttt tttgtgcttt cgtaaactcc cagccctgct
    16681 ccggccctgc ggtcaggata attctcccac ggccctcttc cataccagga aaaacggctg
    16741 acctgctctg gaagctcaaa ggccagtcca aatacaggca gggacggata tcccttcttt
    16801 ccatggaagt gcgctctcac cccaagcata ccgtcctttc gcatcctgta tgtaacagta
    16861 gtatcctccc atgtaaatgt aagctctgct tccttgtctg tttctctgat ctctgccttt
    16921 cctttgcaaa tttccggaaa taatgtcttt gccagccaca gcctgctttc ctttgcaaaa
    16981 ccgtttcccc ggtcattgtc tgtagacgcc ctccagtata caggcacagg aggacggatc
    17041 atccactgta tgccatcctt tctctttaaa gataccagac cgccgtcttg ataggaaaac
    17101 ataagctgca tctctcccag aataacgccc aggtttacat cgccgtcaat cattcgggcc
    17161 ggaatgaacc gttttctttc cattttccag tttcctggaa gaactgcttc cgaaaaagca
    17221 agttcatatc ctttttctgc ccacagcgta tccttttcca aatggacgct tactcttagg
    17281 atatattcct ttccatcttc caacgcggta tcgtttgtaa agcctgtctc atcaaaggaa
    17341 tatacggcgc acgttcccgg agcagcctgt gccttaaacg gaaatgtttt ccattttttt
    17401 ccatttctta acactgtcag cacaaacgca agatccgaaa ggtctgaaaa aagctgccgg
    17461 ttctcaatct gtatctgccg tctctccgga gaaatcttca aacgcaccgc agcatactga
    17521 gcctttacct cagcagcctt tggagatatg gaacgatccg gatacaaaat tccgtttccg
    17581 cagaactgcc agtctgtggg acggtctcca aaatcaccgc cgtatgccgg atattccttc
    17641 ccgtcttttc cctgtctggc aaccgcctga tcgatcatat cccagataaa gcctccctga
    17701 taggcaggat attcatattc caacgccata taccgatcca tgcctcccag ggaatttccc
    17761 atggcatgca tatattcaca cataataaaa ggctttttcg tgttattttc cagaaattcc
    17821 cttacacgtt ccggaggcgt atacatctgg ctttccatgt cactgatatc ctcaaattcc
    17881 cggttccaga aaacgccctc ataatggatt agacgccccg gatctctctc cttaaaccag
    17941 cggctcatcg cacggatatc ctctccggca taggactcat ttccgcagga ccagatcagc
    18001 acagacggat gattcttatc ccgttccgcc atagactttc cacggtctgt tacacaggct
    18061 ttccactgtg gcaggcttcc cggtacattc catgacggct cacaggctcc cattttctgc
    18121 catgaaccat gactttccag atttgcctca tccataacat aaataccata acggtcgcag
    18181 agctcatacc acagggactg attggggtag tggcaggtac ggacggcgtt gatattatgc
    18241 tgctttaaaa aacgaatatc ataaagcata tcatcggctg tgacagccct tcccctgtgc
    18301 cagtcaaatt catggcggtt tatgccgtga aaaacaatgg ggcatccgtt taagcacatc
    18361 tgcccgttct tcagctcaaa gcttcggaat ccaactggct gcaaaaaaga ctccttacag
    18421 ccatcttctc cccacagttc tatatgaagc agatatcgga agggatcttc tgcgctccat
    18481 ggatgtacat tggggatttt tcccagtgat acggcgcagc ttttttccat ccttcctttt
    18541 aaagcccctg tttttatgat ccggcctgca tcctccagcg caagccttat ctcatagctg
    18601 tatccgtttg gattttccat ggtaaattct acatccagaa agccatctct gtaatcatcc
    18661 gtcagatacg cctttataaa cagatcctcc acatggattt ccggtataca gtaaagatat
    18721 accgaccgga aaatccctgt aaaacggaag aaatcctgat cctcaatcca gctggcactg
    18781 ctgcgcttgt aaacgcgcac ctccagccgg tttacgccag gatgaagaac tcccgtaagc
    18841 tcatactccg acggtgtaaa gccgtcctcg ccataccctg caaactgacc gttacaccac
    18901 acataaaagg ctgtttccac tccctggaac gacacaaaca cccgtttttc cagaaaatct
    18961 ttctcctgat ctgtgataat aaattccctt acatagctcc cggccggagc attctcccag
    19021 tcaatggccg gaggacggat ctctgacctt ccatcccatg gatacatggt attgacataa
    19081 tggcagttgt catacccctg aagctggaca tggccgggaa cctggatcgt tccccagtcc
    19141 ttttcctcaa aagcagcttt ataaaaagca actccgtctg aatcctccgt tcccctgagc
    19201 gcatattccg gtggctgctc ctcccagcag aaatcccaga caccatccag aagcataaaa
    19261 ccgattccct ctgatcctgc ctcctgaaaa aaaggatata catgatctga atgagcattg
    19321 ttccggttca cctcaaaaac ctccggatta tccagccagg aaatccttgg atttctactt
    19381 ttcatccgta tttcctcctg ctttatactc aaaattggga atgttctccc aacgctttct
    19441 gaaatagtgt gctgccagct ttggccgtct ttctcttgta aacaggccct tcttattgcc
    19501 gtccactctc atgcaaccct gaaatgccgc aaagtccgca aaattccaga cctgctctcc
    19561 cataaagaaa gaccttttat caaattcctc attctgccgc ctgtaatatt ccatctgata
    19621 ttcttcactg aacatttccg gtacacactg atggatgccc gccacagcgt ctgccccata
    19681 ctctgtaatc atcacaggtt ttcctatttt ttcccagaag tccagttcca tgttcaaagc
    19741 atagcaggct gcatccagat ctcccgacaa gttataccat ccgtaatacc ggttgatgca
    19801 tactacatcc atcgttcggg tcacaagatc cttttcataa ttattctggc agcagacaaa
    19861 ggtcactgga cggtcctgcg gatccagggt gtgcgtatac tcatacaggc tgtgccagta
    19921 ttcataggct gattccggaa aatgctctgt atccggctca tttcccatag accacattac
    19981 cacgcaggga tgatttttat ccctctcgat catatcacgg agcacagcct cgtgatgctc
    20041 cctgatagga aatgtctgat aaggatcgca gtgctcgcca gctccgattc ctacagccgg
    20101 cgtttcatca atgatcacaa ttcccccttt gtcacagagc tgatacatct cctccgcata
    20161 gggataatgg ctagtgcgga aggaattggc atgaagccag tggatcaggt tcacatcctt
    20221 tacatccagg cacagatcca gcccccttcc atgaaatggg gaatcctcat gctttccaaa
    20281 gcctttaaaa taaaatggct ttccattaat caggaactgg gttccttcta ccttcactgt
    20341 acggatgcca aaggtttcct catagatatc ttctccaaag gtaaatacag cccggtacag
    20401 ataaggcgtt cccggccagg gccaccacaa tctggcatca ggtatggtaa tggttccaga
    20461 agctccctct gcctctgcta ccttattccc ctccacatca aaaatttcca catgaacggc
    20521 gccttctccc tctgtctcag cctcatattg aagaatccca tcggtgtcgt ctatgtccgg
    20581 tatcagggtc agaccgcaga tgcgggaaac aggtgttgta tacagccgta ccggacggtt
    20641 gattcctgca tagttaaaaa agtcaaaatt gggaagattg ccctgttttc gccatacctt
    20701 ggctgcttca accgaaggca cacctggatt atcggagcca aaaaaagcgg ttccgccttc
    20761 atttcccact ggaagcgtgc tgtggctgat ccggttgtcc gccgcaacgg taagcagcag
    20821 cgtttctccc ggaaccacaa gctccgtaag ctccagctca aaaggaagaa atcctccctt
    20881 atgttccatt acaaaggttc cgtttatata gatccttgcc ctgtgtgtca cggcatcaaa
    20941 acgcagaaac agcctctgcc ccttaaaaaa ggcaggaacc gtgacctctc tttcatacca
    21001 ggcccatcca tagtgattct tataagccgg atcgtccttc tgatcattat aggatgcagg
    21061 gacagcaatc cgttcccctc ctgcaagacg ctcacagtta tcccccggcg tctcataatc
    21121 tccaagctta aattcccaca ggccggaaag atccatcacg gtccttgctc tgttcatctt
    21181 cggatataac atatcactct tctccttcct ctataccgcc atacgcaaat cccactgcca
    21241 ccaaaaggca caaaaaggtg tttgctctta tagcatcatt atacaggaag aatttctcac
    21301 attacatata ctttatctca aataatctgc aatcttttac acttcctctt ccctcaccag
    21361 catggtagga tatccggcct gtcttactct ctgctccatc cggactccat tgtccatctg
    21421 caaaaaagcg ccgacccgca ccttataaag cccgtcatca tatacaagaa aagccggaaa
    21481 tcccctggct gcaagctccc ttaccagaaa ctctgcccgc ggccgttccc tgtatgctcc
    21541 cacctgaacc tgatagtacc gcttctcttc cgtctctttc ttcagttggg aaaaggtggt
    21601 caatatcccg tctgcaatcc cctgggcgat ctgatcaaat tgttcatcaa aaatcctgtt
    21661 gtcctccggg tgatctaaaa atcccgcctc caccagcaca gccggcatct tagtccgcct
    21721 gagcacaatg attccgggac gttcctgaac tcccagatcg tcaaatccgg ctttcacaag
    21781 atttttctgg atattttctg ccatcaatgc tcccggaccg gtattttcgt ataccaggtt
    21841 caatgctccc tttccggttc ccggaacagg catggcattc cggtgaatgg atacgaaaaa
    21901 atcagctccc gatccattcg caatcatggc tttttccaga ggcgtgtcat atacgtccgt
    21961 ggtacgggta tacatcacac gaatcccatt attttccagt atctcgccta cagccactgc
    22021 cagacggagc gtatcatcct tctctttgcg cccctcaaac atggctcccg gctctgtccc
    22081 gccgtgtccg gcgtctatga tcacactttt ttgctgttcc ataagcagct ccttttacat
    22141 acttcatttc cttataccat atgtaaaaat gagacggatg tttccttggc gccggcggat
    22201 aaacggccag gataccgtct atatctgata tgcgtgatcc acatccacac ctcttggccc
    22261 aagatgagta gaaaatacaa tctgggtact ggtctttcca aacttctaaa gctgtccgat
    22321 aaagccgtcc agttccatcg tactcttaaa ggctgccttc tttcccgtcc tcccaaaaag
    22381 gctctatggc tccgttaaac acctccactg caggccctgt cataaacaga tggccgcttt
    22441 cccggtccag acggatcctt aaatcgccgc ccttaaggct tacaagcacc tcatcctctg
    22501 ttaatcccag caaaatagct gccatggcgc tggccgtagc tcctgtgccg caggcccagg
    22561 tttctccgct gcccctctcc catacccgca tctggatatg tgtacggtca atcacgcgga
    22621 taaactctga atttacacga tccggcacaa aacgctcatg gaactcaaaa gacggtccca
    22681 tcttctccag atccaggctg tctatttcat ccataaaata cactgcatgg ggatttccta
    22741 catcaattcc cgtaaactcc cattgttttc catctaccaa aatcgtttcc ggaagctggg
    22801 aagtgagaac tgcctctccc atgtcaacgg tgagcatcac tgccctgccg ttttcgacct
    22861 ccagttttaa ctccttaatc ccagccggag tctctacggt caggcttctc cgatccgggg
    22921 aaataatccc atggtcatag gcatattttc ccacacagcg gatgccgttt ccgcacattt
    22981 tcccctgtga accgtctgta ttgtacattt ccatacggca gtctgccaca tcagaagggc
    23041 agatcaggat caggccgtct gaaccgatgg caaacctccg gtcgctgata aatcgggctg
    23101 ctgcacttgg attttttacg ttctccttaa aacagtctac atatacatag tcatttccaa
    23161 ttccctgcat ttttacaaaa ttcatggttc tacctcacat gtgtattaag ctgatgatca
    23221 tctgagcagt ttcggtcagg ctggtgccat tatccatact gcatttttgt atatatcggt
    23281 aggcttccgg ctcggtcatc gtatttcgat ccatcaggag agcttttgcc tttaaaatca
    23341 gttccttttc ctcggcgctt ctctcctgag gacgggtcct ctttctgcgt tttcgccgtt
    23401 ttctggcctc aagcatcatt tctatggtgc ttaaaagctc atgtaccttt aagggcatgg
    23461 aaaggcagat cattccctcc gtatcccgtt tgctccattt tgccggggaa gcaatgagaa
    23521 gcatatcaaa ggaggatggg agcaggcggc gcagctcctc gcagaccata tcttcaaagc
    23581 ggaagccgca gactacgatc ccgccgttta aactctctgc cgctgccaga acctgagccc
    23641 cggaggtgca cgcaaaaact ggttcatagc cattcttcct gagtatggcc cggatatttc
    23701 cggcatcctg tggctttgaa aatgccgcga ttatacttgc catccttccc acctcctcct
    23761 gccaggctgc acactaatat tgatacagat attgggcgat ctcccagtcc gtcacctgaa
    23821 tgccgaactc tccccattcc ttctctttgg cctccagata tttaatataa atcggttctc
    23881 cgagaacatt tttcacaaaa agactgcctg catatgcctc aatggcagct ccaagagtgt
    23941 ttgggatccg gtcaatcccg cagttattaa gttcctcctg atcagccaac gatatcggtt
    24001 cttttgcaca gagaggcagc tccagctcct tttcaatccc ctccagtccc gcctgaaggc
    24061 aggctgcaag tgccaggtat ggattcatgg ccccatccgg acagcgcagc tcaatcccct
    24121 ctcctcccgg agacggaatg cggatcaggc agcttttgct gtttgaaggc gaccaggcaa
    24181 tataaaccgg ggcatcatat ccaggaacca gccgcttata ggaattaacc gtcggattgg
    24241 taagaagcgt catttctttc atatggttta aaattcctgc cataaaatg
//
LOCUS       DBMDUH010000023        22814 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_1471_length_22814_cov_8.430414, whole genome shotgun sequence.
ACCESSION   DBMDUH010000023 DBMDUH010000000
VERSION     DBMDUH010000023.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 22814)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 22814)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22814
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1471_length_22814_cov_8.430414"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="ACZPCG_03540"
     CDS             complement(<1..218)
                     /locus_tag="ACZPCG_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284179.1"
                     /translation="MDRHSSYTAPRSDRSPASRGSRRSPKTSNNGRSGNENRFLHNLL
                     WYVLPFIVFNLLLLFLVTASPRIELTVG"
     gene            458..997
                     /locus_tag="ACZPCG_03545"
     CDS             458..997
                     /locus_tag="ACZPCG_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR1284180.1"
                     /translation="MIEEAAAFAAQAHHGTFRKGTQIPYITHPMEAALIVSGFTDDPE
                     MIAAALLHDVVEDTGVTEEEIKERFGSRVACLVANETEDKTKCWRERKGATIQHLKSA
                     PREIKILALGDKLSNMRCTAKDYLVFGDEIWQRFNEKDKAMHAWYYWGIADALDDLKE
                     HLYYKEYIMLCRTVFGAGT"
     gene            1078..2460
                     /locus_tag="ACZPCG_03550"
     CDS             1078..2460
                     /locus_tag="ACZPCG_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR1284181.1"
                     /translation="MSNLKRNGRILALLVIMGILFGTPGMTALAGTMGVGAAAGPAGG
                     PGMEAVGPSDQNDEQEKPSETDGVQSAEGQPAGGSEAQGESGQSAAGSEIQDTADHST
                     ASSDLPLQVNYSIYSPIQGWSGTAADNQALQAPYGSWVAAMRANLINIPADAQVGIRY
                     QVNLSGTGWLDPAEDGAEAGNASAEAPLEAICMELYGASAASYDLYYKVYQNGTWTDW
                     ALNGAVAGTEGAGLRIDGIRAAITAKDAGAPQAAMNAVDPSRPMIALTFDDGPRTSVT
                     SRILDSLQQNGGRATFFMVGTNVDSNAAVIRRMAEQGCEVANHTNDHKYLTKIGAEGI
                     RSQVGSVNQKIASACGVSPVLMRPPGGYVDGASLSVLGSMGMPAIMWSIDTRDWQHRN
                     AQRTIDTVLDQVKDGDIILMHDIYEPTAQAAEVLIPELTARGYQLVTVSELASFRGGI
                     QPGHKYSQFR"
     gene            2728..3723
                     /locus_tag="ACZPCG_03555"
     CDS             2728..3723
                     /locus_tag="ACZPCG_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284182.1"
                     /translation="MNEYKNRRQLLCLAVTVGMMFTAVNVSSKTVWAAAEQVIEYDPN
                     QEEKTHPGSIILKQNLKEEDLPSLKQPEASYTDENGIHYRLADWEVRELPEASRSRML
                     EKTEVYREVEGAETLPPYVDSRQEENGAKISGRLYMQESRVLGEEWREDFQAPVTFYS
                     YGADEYELGGIKISGEDILDSALAEQELLLGEMGLSSEAYHITSMDWDGESFMDETGQ
                     VCRRASARGERLLRDYEAVYRGQVQVVEPVSYEVSAVYEPAELGNISNSEKEQEEALV
                     LEPEAAEEPDPFLRWIRTGVTVTAALGVSGILLGIVLYFTGKNRRKSQEEELKYN"
     gene            3845..4693
                     /locus_tag="ACZPCG_03560"
     CDS             3845..4693
                     /locus_tag="ACZPCG_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284183.1"
                     /translation="MKRKYFMIAVMAAALGMTACSSQKTETEAATATTAASSAAETTT
                     AEASSEEEEAETESFYGIVKTVEDTVITVTDDAGTEAGFDVSGAELTGAEAIGEGDEV
                     DVYYTGALGETPAKAVEVDIVISAAEEAEEEAAEEDDLTITGTIESTEDGSLVLKTED
                     GSYTLNTLIAQTVTKDGVKAGTEADITYYGDLEDEEVPAVATKIVTADARDTEDAGIK
                     TLTGTVAEAESNHVVLDTEDPENTFFSFVGEDGMFDGLKTGDTATVIYEGTLTAKTIK
                     ALGLKK"
     gene            4867..7458
                     /gene="clpB"
                     /locus_tag="ACZPCG_03565"
     CDS             4867..7458
                     /gene="clpB"
                     /locus_tag="ACZPCG_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007719260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HIR1284184.1"
                     /translation="MNINKFTQKSMEAVQGCEKLAYEYGNQQIDQPHLLYSLLTLDDS
                     LIMKLVTKMGIQGDMFKNEAKQAIEKLTKVSGGGQLYISNDLNKVLINAEDEAKAMGD
                     EYVSVEHLFLALLKQPGREMKELFKTYGITRERFLQALSTVRGNQRVVSDNPEATYDT
                     LEKYGYDLVERARNQKLDPVIGRDSEIRNVVRILSRKTKNNPVLIGEPGVGKTAVVEG
                     LAQRIVRGDVPEGLKDKKLFALDMGALVAGAKYRGEFEERLKAVLEEVKKSEGQIILF
                     IDELHTIVGAGKTEGSMDAGNMLKPMLARGELHCIGATTLNEYRQYIEKDAALERRFQ
                     PVMVAEPTVEDTISILRGLKERYEVYHGVKITDSALVSAAMLSDRYISDRFLPDKAID
                     LVDEACAMIKTEMDSMPAELDELSRRLMQMEIEEAALKKETDRLSQDRLADLQKELAE
                     LHDEFTAKKAQWENEKSSVDRLSALREEIEAVNRQIQDAQQKYDLNKAAELQYGKLPQ
                     LQKELEAEEEKVKKEDLSLVHESVTEDEIARIVSKWTGIPVAKLTESERSKTLHLDQV
                     LHKRVVGQDEGVEKVTEAIIRSKAGIKDPTKPIGSFLFLGPTGVGKTELAKALAESLF
                     DDESNMVRIDMSEYMEKHSVARLIGAPPGYVGYDEGGQLTEAVRRKPYSVVLFDEVEK
                     AHPDVFNVLLQVLDDGRITDSQGRTVDFKNTIIILTSNIGSQYLLEGIDENGSIRPEA
                     KEAVMNDLRTHFRPEFLNRLDEMILFKPLTKENIGSIVDLCIADLNRRLADRELKLEL
                     TETAKAFITENGYDPVYGARPLKRYLQKNVETLAAKLILGGGLREGAVIVIDVSEDGS
                     RLIAYTE"
     gene            7479..7658
                     /locus_tag="ACZPCG_03570"
     CDS             7479..7658
                     /locus_tag="ACZPCG_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4250 domain-containing protein"
                     /protein_id="HIR1284185.1"
                     /translation="MNKIPKDPMILFSFVNTQLRDRFSSLEEMCREMELDQSYIEETL
                     EGAGFSYDPKRNCFR"
     gene            7874..9406
                     /gene="cls"
                     /locus_tag="ACZPCG_03575"
     CDS             7874..9406
                     /gene="cls"
                     /locus_tag="ACZPCG_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009297419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HIR1284186.1"
                     /translation="MGVIFNEAEAVWEWLMTHLIVINLLFSIIIIFFQRRDPRSVWTW
                     LFALNFIPVFGIIFYLLFGQDLKKSRMFKVKGISDRLNMPVKSQEEIIRSSDIPEEMM
                     DPLIRDFRSLILYNLETSGAVLTTDNQVKIYTDGQAKFEGLRTALRRASRFIHIQYYI
                     IKNDEVFDSIVPILEDKVREGVEVRILYDGMGGRFMPKRRWEELRRAGILVGVFFPPI
                     LGWLNLRVNYRNHRKIVVIDGNVGFVGGFNIGREYISKDPGFGYWRDTHLRITGEAVM
                     SLQIRFALDWNYATGENLFKNEAYFRTDLKKKTDVRRDKERPAWPDPELEGSQVGMQI
                     IASGPDTREPHIRNNYLELFHKAKHHIYIQTPYFVPDDAIFSALKMAALSGVDVRLMI
                     PCKPDHPFVYRATCSYAGELLDYGAKVYIYTNGFLHSKGIMVDGRVCCYGTANMDIRS
                     FELNFEVNATIYDEAVTAELEQAFMNDLPHCRQITREDYKKRGLLTRIREQGSRLLSP
                     LL"
     gene            9445..9936
                     /locus_tag="ACZPCG_03580"
     CDS             9445..9936
                     /locus_tag="ACZPCG_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HIR1284187.1"
                     /translation="MKRQYNGKLKESLRNFCRCGIAGWCLEVIFTSSESWMRGDMRLM
                     GRTSLWMFPIYGMGVLLPGIVNTADRWLTGLSAFPEAGTDRISPPARLVRHGLMDMVL
                     IFTAEYVTGTILKHFNVCPWDYSMWPDHIGGVIRLAFGPLWFCTGLLFEMLQTPGRLE
                     NSN"
     gene            10052..10792
                     /locus_tag="ACZPCG_03585"
     CDS             10052..10792
                     /locus_tag="ACZPCG_03585"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR1284188.1"
                     /translation="MIRLLLAAIAIVLYLVLTFPVLCFLEKKAKKNPEEAGWKSFSMV
                     RWIFKKISRIAGITYEVRGLENIPTDRAVLYVGNHRSYFDIVMGYMTVPGLTGFIAKK
                     EMIKIPLLNRWMDRMNCLFLDRSNIKEGLKTILAGIEKIKGGISMWIFPEGTRNPGEN
                     PEKLMEFHEGSLKIAEKSGCPVIPVAMVGTAEVFERHFPWIRPSHVIIEYGKPIYLKE
                     LPAENRKFPGAYTREIICSMIKELEEQR"
     gene            10789..11925
                     /gene="pheA"
                     /locus_tag="ACZPCG_03590"
     CDS             10789..11925
                     /gene="pheA"
                     /locus_tag="ACZPCG_03590"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HIR1284189.1"
                     /translation="MKELNLQEIRQQLDVIDASLTELFETRMELCREVAEYKIQTGKP
                     VYDREREQQKLKAVTALAHSDFTRKGTYELFSQIMTISRRLQYGLLASHGQEMDTGFS
                     MIEDLKRENVRIVYQGVEGSYGHGAARRYFGPDADMYHVKTMEDAMTEVEEGRADYAV
                     LPIENSSAGAVSDNYDLLVKHNVYIAAETELPINHALLGREDADIKDIRTVYSHPQAL
                     MQTSQYLNAHRDWKQISTVNTAVAAKKVAEENDRSQAAVASEIAGELYGLKVLERSIN
                     HNKNNVTRFIILSKDPVYRKGANKVSICFEGVHRSGSLYNMLGNLIYNDINMLMIESR
                     PIPGRSWEYRFFVDVEGSLSDAKIRNALKGISEEAVSLRILGNY"
     gene            12008..13282
                     /locus_tag="ACZPCG_03595"
     CDS             12008..13282
                     /locus_tag="ACZPCG_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006770952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1284190.1"
                     /translation="MDIRGLVIYRNLKHQTLFEQMAGLMGMSEEKESTDSFACAGQLI
                     ELAAKYGFEGNLWHCFLALCLADNENAYTTACEVKGPAGGTLDLLAKEDFAIFKALFD
                     FDLSSLAPSSLWSLLADYRPALQESRVFNRRIRDRIVELAKALEQASDLEGFQQTMVE
                     FYRGYGAGTFGLNKAFRILEKEAGGLTVIDPIVNVEHIYFKDIVGYELQKHKLIENTE
                     AFVNGKEANNVLLFGDAGTGKSSSVKAVLNEYYSRGLRMIEVYKHQFKDLSDVLEQIK
                     DRNYKFIIYMDDLSFEDYELEYKYLKAILEGGLGKRPDNVLIYATSNRRHLIREKFSD
                     KRELDDDLHNNDTVQEKLSLAARFGVTIYYGSPDKRQFQSIVKALAKRHQLDIPEEEL
                     LLEANKWELSHGGLSGRTASQFITHLLGCDFT"
     gene            13408..14973
                     /locus_tag="ACZPCG_03600"
     CDS             13408..14973
                     /locus_tag="ACZPCG_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HIR1284191.1"
                     /translation="MAVRMFAAIEIGSFALELGIYEISEKTGIRCVDHIRHVIALGSD
                     TFSEGKISYGLADEMCSVLEDFTGIMKTYRVREYRAYGTTAMREAKNSQIILDQICVR
                     TGLKVRIISNSEQRFISYKAVAAKTGEFNRIIQKGTAIVDVGFGSAQISLFDKDTLVT
                     TQSLPLGTLRIRSQLAKIPASVRSHCLHIEELVDNELITFRKMYLKDRKIDNLIGIGT
                     NITYLMRQLGFNTAADRVDAKEMELFYERLSQMTLDQIEERFNVNSEYASLLLPAAVV
                     YRRVMEVTGAGQFWIPDIGLCDGIAAEYADDCRLFRFSHSFENDILAAARNMAKRYKC
                     HAGHNQMLEQYALSIFDAARKFHGMGARERLLLQIAVTLHGCGKFISIRNSNESSYNL
                     IMSTEIIGLSHLEREIIANVVRYNIRDFDYDTIQLETQIGEDGSGNLNRSSITILIAK
                     LTAILRLANSMDKTHKSKLADCRMAIREGELVITTGYTGDLALERASFEQKADFFEEI
                     FGIRPVLKQKRRA"
     gene            14975..17143
                     /locus_tag="ACZPCG_03605"
     CDS             14975..17143
                     /locus_tag="ACZPCG_03605"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007719206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HIR1284192.1"
                     /translation="MAEKEKNTTDFGADPSNFVNRELSWLEFNYRVLSEARDKSLPLF
                     ERLKFLSITASNLDEFYMVRVASLKDMVHAKYMKQDIAGMTAQEQLDAISIKTHQLVE
                     LQYSTYIRSLVPSLRQNGLRIIMEHEDLTKEEAACADSYFQTNVYPVLTPMAFDSSRP
                     FPLIRNKTLNIAALLKKKNGDGELEFAMVQVPSVLPRIVELPRETMEDGTEQRVVILL
                     EEIIERNMPSLFLNYDIVASHPFRIMRNADLSIDEEEAVDLLEEIQKQLKKRQWGEAI
                     RLEIESNVDKRLLKILKKELSISSQDIFRIPGPLDLTFLMKMYGLEGFEAFKAPKYIP
                     QPVPALMNEDDIFTNIRKGDILLHHPYQSFDPVVDFVRSAARDPEVLAIKQTLYRVSG
                     NSPIIAALAEAAENGKQVSVLVELKARFDEENNINWAKMLEKAGCHVIYGLVGLKTHS
                     KITLAVRMEEDGIRRYVHLGTGNYNDSTAKLYTDCGILTCDPQIGEDATAVFNMLSGY
                     SEPKAWNKLSVAPLWLRNRFLRMIRRETAHAKEGRRGHIMAKMNSLCDKEIIKALYEA
                     SCAGVEIELVIRGICCLRAGVPGLSEHIHVRSIVGNFLEHSRIFYFLNDGSPEVYMGS
                     ADWMPRNLDRRVEILFPVEDEALKEQVIHILKVELEDNVKAHILQPDGTYEKEDKRGK
                     VLINSQEQFCLEAVQRVKEYRGEDDPAVTRIFKPIESGNI"
     gene            17311..18015
                     /locus_tag="ACZPCG_03610"
     CDS             17311..18015
                     /locus_tag="ACZPCG_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008724114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1284193.1"
                     /translation="MPDINILVVDDEKEIADLIEIYLVSDGYKVFKAENALKGLEILE
                     KEEIHLVLLDIMMPGMNGLEMCKKIRETNNIPIIMLSAKSTDLDKILGLGTGADDYVV
                     KPFNPLELTARVKSQLRRYTQLNPNSMAKEAVSSEITIKGMSINKDNHKVVVDGEEIK
                     LTPIEFDILYLLASNPGKVFSTDEIFERVWNEKVYEANNTVMVHIRRLRGKMKEDTRQ
                     SKIITTVWGVGYKIEK"
     gene            18017..19135
                     /locus_tag="ACZPCG_03615"
     CDS             18017..19135
                     /locus_tag="ACZPCG_03615"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005540221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1284194.1"
                     /translation="MGSSYRFRVLTHIVYSAIITCLVEVFLITNLSMVARYIQQSGKV
                     SALVHFILEQHLAVVILYVLVGIVIFSVTFMILEEPGIRYLGRISDAVQSISQGNLNT
                     EVDVTGDDEFSAMAANLNKMSSDIRKLMDKEREAERTKNELITNVAHDLRTPLTSIIG
                     YLELLAGNTQIPQEMQHKYIEIAYSKSRRLEKLIEDLFGFTKLNYGKIAMHIGQIDIV
                     KLLEQLLEEAYPNFEEKNLSYDLQSNVPAKIISADGNLLARLFDNLIGNAIKYGADGK
                     RVLVKIHGEEDTVTVSVTNFGRVIPADELPLLFNKFYRVEQSRSATTGGTGLGLAIAK
                     EIVDMHGGTIRVASDLNGTVFTVKLQVHFDLEKEQFGS"
     gene            19171..21738
                     /locus_tag="ACZPCG_03620"
     CDS             19171..21738
                     /locus_tag="ACZPCG_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284195.1"
                     /translation="MYRYIRVLKGALFALLFSLSALLPSYAAITADGPGSGVAGPRET
                     VPEEGAQTEALWQDPASPHPLEMEVVYGYDNTARSGRILPMTIRVTNSTKEDFTGTLR
                     AATLEPEYSPYGNSSLSEYSTYRYEYPIEVKAGETVEKKIQISLSIQVDQVLLSLVDQ
                     EGKETAGRRIRLDLNLDTAELYVGVLSDHPERLSYMDHMGINYSTLRTRLIELDQDTM
                     PESRQELDQLDVLLINDFDTGSLDSRQLEAVWEWTQSGGTLLFGTGEHANETLGAFSK
                     ALLASPLPQAVEFELDMESSQESSEYGDSRIVLNCTEVDLKGSTEVMTKGPMEIISST
                     PVGRGMAAAAIYDFGDLKEYGTDNPSYVDRLFTGLIGENRLNLLSNGNGGSSTGRYWE
                     VQSLINTGNVNRLPKVGLYVTLAVAYVALAGPGLYFFLKQRELRQYYQPAVAILSLCC
                     TGMVLLMGIPTRFNGPFFTYASIQDAGEEDVSETLFINMRSPYNHSYGVSLDPSYRLF
                     PVCDSSYYNPGTVPALVDSEDPDMVIRYKEDETYIEARDTGAFNPSYFQMERNLPNET
                     GQGFSGEIRAFEGTITGTITNNYPWTVENAALLLYNQMVMIGTIEPGQTISLDGRELI
                     YCATDLGYAMAAQITGASRYGQKVNIEDPDYVRALERTNLLSFYVENYFSGYHTQARV
                     VAFSQEQKETGFLRNPGTETYGCTLLTSELDVNYEQDGLVSRSAMQKQPHVLAGEYDA
                     ARNTIYGINPVVLEYYLGNELEVDTLHFHRLSEGVVANLRYYYTVPFEGNMYFYNYNT
                     GSYDRMDSAVSQYNREELDSYLSPGNTITVKYVYDTAGEYTWNIMLPVLTVTGRRQP"
     gene            21738..22706
                     /locus_tag="ACZPCG_03625"
     CDS             21738..22706
                     /locus_tag="ACZPCG_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1284196.1"
                     /translation="MLTVRNLHKIYGKYHALSGLDMNVGDGALYGFVGPNGAGKTTTL
                     KIMTGLLGAEEGSVTINGKDALRDLGELKGMIGYVPDFFGVYDNLTVAEYMSFFASCY
                     QLDGLMARKRYTALLEQVGLEDKLDFYVDGLSRGMKQRLCLARALIHDPAILILDEPT
                     SGLDPRTRFEFKEILSELRSTGKTIIISSHVLSELAEMCTDIGIIDQGRMVLEGNITA
                     ILSRVNASNPLVISVYSNMEQAMAVLRRHPCVQTISMREKDICVRFAGDGTDEAMLLQ
                     QLIDAGVLVNGFVREKGSLESVFMQLTDHEEERVVTSYEAESGLQP"
BASE COUNT         6285 a         4688 c         6429 g         5412 t
ORIGIN      
        1 tcccctacag tcagctcgat tcggggggaa gcggtcacca gaaagagcag aagaagattg
       61 aaaacaataa acggcagcac ataccataaa agattgtgaa ggaaccggtt ttcatttccg
      121 gaacggccgt tattgctggt tttggggctg cgccgtgagc cgcgtgaggc cggtgaacgg
      181 tcgctgcggg gcgctgtata ggatgaatgg cggtccattg cctttgtcct cctttgaagt
      241 ttggtcaaaa taccactata gcatagcaga aattgtctgg tcttacaagg aaaaattgct
      301 cttgtaagag tgttgtaaga aagtctgtgg ggaaaaggga tgaaaacagg agcagaatat
      361 gatatgatga aaatgatact acatatgcca gaatattccc ggaacgggaa gggcacagga
      421 tgatcctcag gggtccagcg agaaaagggg cagagccatg atagaagaag cagcagcatt
      481 tgcggcgcag gcgcaccatg gaacattcag aaaagggaca cagatcccgt atattacaca
      541 cccgatggag gcagctctga tcgtatccgg gtttacagat gatccggaga tgatcgcggc
      601 agcgcttttg catgatgtgg tcgaggatac gggcgtgaca gaagaggaga ttaaggagcg
      661 cttcggttca agagtagcct gtctggtggc aaatgagaca gaggataaaa caaaatgctg
      721 gagggaacgg aagggagcta ccatacagca tctgaaaagt gctccaagag agattaagat
      781 cctggctctt ggggataagc tcagcaatat gcgctgtacg gcaaaggatt atctggtttt
      841 tggggatgaa atctggcagc ggtttaatga aaaggataag gccatgcacg cctggtatta
      901 ctgggggatt gcagatgcat tggatgattt aaaggaacat ctttattaca aagaatatat
      961 tatgctctgc cggactgtat tcggagcggg gacatgaggt caatgaagca ggaagtccat
     1021 tggctttagg cgatggacgg tctactggaa gcggcagaca gtaaggagaa tgaaagaatg
     1081 agcaatttaa aacgaaacgg caggattttg gcgctgcttg tcataatggg aatcctgttt
     1141 ggaaccccgg gaatgacggc acttgcagga accatgggag taggggcggc agcaggtccg
     1201 gccggcgggc cgggcatgga agcagtgggc ccatcagatc agaacgatga gcaggaaaag
     1261 ccgtctgaaa cagatggggt acagagcgcg gaaggtcagc cggcaggcgg atccgaagcc
     1321 cagggggaat ccggacagtc cgctgccgga tccgaaattc aggatacagc ggatcattca
     1381 acagcctcat ctgacctccc tcttcaggta aactacagta tttactcccc gatccaggga
     1441 tggagcggta cggctgcgga taatcaggca ttgcaggctc cttatggaag ctgggtggca
     1501 gctatgaggg cgaatctgat caatattccg gcagatgccc aggtaggcat ccgctatcag
     1561 gtaaatttaa gcgggaccgg ctggctggat ccggcagagg atggagcgga ggcgggaaat
     1621 gcgtctgctg aggcgccgct ggaagcgatc tgcatggagc tgtatggagc gtctgcagca
     1681 tcatatgatc tgtattataa ggtttatcag aatggtacat ggacggattg ggcattaaat
     1741 ggagctgtgg caggtacgga gggagctggc ctgaggatcg atggaatcag ggctgcgatc
     1801 acggcaaagg acgccggagc gcctcaggca gccatgaacg cagtcgatcc ctccagacct
     1861 atgatcgccc tgacctttga tgacggcccg cggacttcgg ttaccagccg gatccttgac
     1921 agcttgcagc agaatggggg acgggctaca ttctttatgg taggtaccaa tgtggactcc
     1981 aatgccgcag tgatccgccg tatggccgaa cagggctgtg aggtcgcaaa ccacaccaat
     2041 gaccataaat atcttacaaa aattggggcg gaaggcattc gcagccaggt aggttctgtg
     2101 aatcagaaga ttgcttctgc ctgcggtgtg tctccggtac tgatgcgtcc gccaggagga
     2161 tatgtggacg gcgcatcact gtctgtatta ggctctatgg gtatgccggc aattatgtgg
     2221 tctattgata cgagagactg gcagcaccgt aatgctcaaa gaaccattga tacggttctg
     2281 gatcaggtaa aggatggaga catcattttg atgcatgata tttatgagcc aacggcacag
     2341 gcggcagaag ttctgattcc tgaactgaca gccagaggtt atcagctggt aacagtcagc
     2401 gaactggctt cgtttagggg cggtatccag cccggacata aatacagcca gttccgttaa
     2461 aaatacttgc aatttgaagc atacatattt ataatgtctt taccgggcgg agttccctcc
     2521 gtccggatct attttctaaa gtcttcaggg ttttatacat cagattcatt catttcttaa
     2581 atttccaaag gtctcccaca ccaggatatt tgatttgaaa acagaaccgc acattggcaa
     2641 ttagataaaa gcagaataca ctgcaaaagg cggaaagcca aacaggtatt ttatgtaaat
     2701 cagataaacc aaaacggggg aacaaatatg aacgaataca aaaacagaag gcagctttta
     2761 tgtctggcgg tcacggtggg aatgatgttt actgctgtca atgtatcatc caaaacggtc
     2821 tgggccgctg cagagcaggt gatcgagtat gatccgaatc aggaggaaaa gacacatccg
     2881 ggctccatta ttttaaaaca gaatttgaag gaggaagact taccgtcgtt gaaacagccg
     2941 gaagcttcct atacagatga aaatgggatt cattaccgcc tggcggactg ggaggtgcgt
     3001 gagctgccgg aagcttcccg gagccgtatg cttgaaaaaa cggaagtgta ccgggaggta
     3061 gaaggagcag agacgcttcc accctatgtg gacagcaggc aggaggaaaa tggcgcaaag
     3121 atatcaggac ggctgtatat gcaggagagt cgggtactgg gggaggagtg gagagaggat
     3181 tttcaggcgc ctgtgacatt ttattcctat ggagcagatg aatatgaatt gggtggaata
     3241 aaaatttccg gggaagatat cctggattca gctttggcag aacaggagct tcttttagga
     3301 gaaatggggc tttcgtccga ggcttaccat atcacatcta tggattggga tggggaaagc
     3361 tttatggatg aaacgggcca ggtctgccgc agggcgtcgg caaggggaga gaggctgctg
     3421 cgtgattacg aggctgtata cagaggacag gttcaggttg tggagccggt ttcctatgag
     3481 gtgtccgctg tgtatgaacc tgcggaactg gggaatattt ctaattcaga aaaagagcag
     3541 gaagaggcat tggttttaga gccggaagcg gcagaagagc cggatccatt tttacgctgg
     3601 atccgaaccg gagtgactgt tacggctgca ttgggagttt caggcatatt gttggggatt
     3661 gttctgtatt ttacagggaa aaacaggcgt aaaagccagg aagaggagct gaaatataat
     3721 tagagcttaa tgattttttt atagaattct aaagaaaatt tcaaagaaaa tacatagtaa
     3781 aaacacagat tacgtagtaa tatgtatcgt gtatacagaa atgaatggag aggagagttc
     3841 atttatgaaa agaaaatatt ttatgatcgc agtgatggca gctgcccttg gaatgacagc
     3901 ctgttcatct cagaaaacag agactgaggc agcaacggct acgacagccg cgtcttcggc
     3961 agcagagaca accacggcag aggcttcttc cgaggaagag gaggcagaga cggagagctt
     4021 ttacggtatt gtaaagacag tagaggatac ggttattaca gtaacagacg atgccggaac
     4081 agaagccgga tttgacgtgt ccggagcaga gcttaccgga gcagaggcca tcggtgaagg
     4141 cgatgaggta gatgtttact acacaggagc gctgggagaa acgccggcaa aggccgtaga
     4201 ggttgatatt gtgatttccg cagcagagga agccgaggag gaagctgctg aggaggatga
     4261 tctgacaatt acgggaacca ttgaaagcac agaagatgga agccttgttt taaagacgga
     4321 ggacggttcc tataccttaa ataccctcat tgcccagacg gtcacaaagg acggcgtcaa
     4381 ggccggaaca gaggcagata ttacatatta tggagatctg gaagatgagg aagttcccgc
     4441 agtcgccaca aaaattgtga ctgcagacgc cagagataca gaggacgcgg gaattaagac
     4501 ccttaccggt accgtagcag aggcagagag caatcatgtg gttctggata cagaggatcc
     4561 ggaaaatacc ttcttttcat ttgtgggaga agatgggatg tttgacggct taaagaccgg
     4621 agatacggct actgtgatct atgagggtac attaacagcc aagaccatta aagcattagg
     4681 acttaaaaag taaataattt cttttgcacc cgcctgttgg aatcgttccg gccggcgggt
     4741 gtctgcatat aaaggattct gtaaaaaaat tagcactcga ctcttgacac tgctaataat
     4801 gtgtgttata gtacgcatag aacaaagaac agaaacacga ttccgcagca gaaaggggaa
     4861 tggcgtatga atatcaataa atttacacaa aagtccatgg aggcggtgca gggctgtgaa
     4921 aagctggcgt atgagtacgg caatcagcag atcgatcagc cgcatctgct ttacagtctc
     4981 ctgaccttgg atgacagcct gatcatgaag ctggttacga aaatgggaat ccagggagat
     5041 atgtttaaaa atgaggccaa acaggccatt gagaagctta caaaggtaag cggcggcggc
     5101 cagctttaca tcagcaatga tttaaacaag gtactgatta acgctgagga tgaagcaaag
     5161 gctatgggag acgagtacgt ttcggtggag catttgtttt tagcactttt aaagcagccc
     5221 ggacgggaaa tgaaggagct gtttaagact tatggaatca ccagagaacg gtttttgcag
     5281 gcgttgtcca ccgtccgggg caatcagagg gtagtcagcg acaacccgga ggcgacctat
     5341 gatacactgg aaaaatatgg ttatgacctg gtagagcggg ccagaaacca gaagctggat
     5401 ccggtgatcg gccgcgacag tgaaatccgc aacgtagtcc gtattctgtc cagaaagaca
     5461 aaaaataatc cggtgctgat tggtgagccc ggcgtaggta agacagccgt tgtagaaggt
     5521 ctggctcaga gaatcgtccg gggcgatgtg ccggagggct taaaggataa aaagctcttt
     5581 gcccttgata tgggagcctt agtggcagga gccaagtata gaggcgaatt tgaggagcgt
     5641 ttaaaagcag ttctggaaga ggtaaaaaag agcgaaggcc agatcatcct ctttattgat
     5701 gagcttcata ccattgtggg agcaggaaag acagagggat ccatggatgc gggcaatatg
     5761 ttaaagccca tgctggcaag aggcgagttg cactgcatcg gcgctactac cttaaatgaa
     5821 taccgccagt acattgaaaa ggatgcggcc ttagagcgcc ggttccagcc ggttatggtg
     5881 gcagagccta cggttgagga caccatttcc attctcagag gactgaagga gcggtatgag
     5941 gtttaccatg gcgtaaagat cacagattcc gctctggtaa gcgcagccat gctgtccgac
     6001 cgctatattt ccgaccgttt tctgcccgat aaggccattg acctggtaga tgaggcctgt
     6061 gcaatgatca aaactgaaat ggacagtatg cccgcagaat tagacgagct gtcccgcagg
     6121 ctgatgcaga tggaaatcga ggaagcagca ttaaaaaagg aaacagaccg cttaagccag
     6181 gatcgtctgg cagatttaca gaaggagctg gcagagctgc atgatgaatt taccgcaaaa
     6241 aaggctcagt gggaaaatga aaaatccagc gtagaccgcc tctctgctct ccgtgaagag
     6301 attgaggctg taaaccgcca gatccaggat gcccagcaga aatacgatct gaacaaggcg
     6361 gcagagcttc agtatggaaa gctgccccag ctgcagaagg agctggaggc agaggaagag
     6421 aaggttaaaa aggaagatct gagcctggtt catgagagtg tgacagagga tgagattgca
     6481 agaattgtgt ccaaatggac cggcattcct gtggcaaagc ttacagagag cgagcgcagc
     6541 aagacccttc atctggatca ggtgctccat aagagagtcg taggacagga cgagggcgtt
     6601 gagaaggtca cagaggccat tatccggtca aaggcaggaa ttaaagatcc tacgaagccc
     6661 attggttcct tcctgttttt aggccccacc ggcgtaggta aaacagagct tgccaaggct
     6721 cttgcagagt cactgtttga tgatgaatcc aatatggtgc gcattgatat gagcgaatat
     6781 atggagaagc attctgtggc acgtctcatt ggagcgcctc caggatatgt gggctatgat
     6841 gagggcggac agcttacaga ggctgtgcgc cgcaagccct acagcgtagt gctgtttgat
     6901 gaggtggaaa aggcccatcc ggatgtattt aatgtgctgc tgcaggtact ggatgacgga
     6961 cggatcacag attctcaggg acgcacggtg gattttaaaa acaccattat cattcttact
     7021 tccaacatcg gctcccagta tcttttagag gggattgatg agaatggctc catccgcccg
     7081 gaggctaaag aggctgtgat gaatgacctg agaactcatt tccgtccgga gtttttgaac
     7141 cgtctggatg aaatgattct ctttaagcct ctgacaaagg aaaatattgg aagtattgtg
     7201 gatttatgca ttgcggactt aaaccgccgt ctggcagacc gggagttaaa gctggagctt
     7261 acagagacag cgaaggcctt tatcacagaa aacggctatg atcccgtata tggcgcccgt
     7321 cctttaaagc ggtatctgca aaagaatgta gaaaccctgg cagcaaagct gattttagga
     7381 ggagggctgc gggaaggcgc tgtaatcgtg atcgatgtat cggaggatgg aagcaggctt
     7441 atcgcttata cggaataaaa agaaaggaag gtcataaggt gaataagatt ccaaaggatc
     7501 ccatgatcct gttcagcttt gtgaacacac agcttagaga ccgtttcagc tcattggaag
     7561 agatgtgcag agaaatggag ctggatcagt cttatataga agaaactctg gaaggtgcag
     7621 ggttttccta tgatcccaaa cggaactgct tccgctgatg gttggcagca aaagccggct
     7681 ttatctgatg tctggtcaga tagggccggc ttttttgagg tggtgcgaaa gggcgaaggc
     7741 tggaaataaa taacaggatg tggtagactg gataaaagat cattcacgga atagggatgt
     7801 atattgtgat gggggatctg ttaagctata gtaggaatac ccaatagagg atgaacaaag
     7861 caggaggaga tatatgggag tgatattcaa tgaggcagag gctgtctggg agtggcttat
     7921 gacgcatctt atcgtaatca atctcttgtt ttccattatt attatttttt tccagcgcag
     7981 agatcccaga tcggtatgga cctggctgtt tgctctgaac tttattccgg tgttcggaat
     8041 tatattttat cttcttttcg gccaggattt aaaaaagagc cggatgttta aggtaaaggg
     8101 aatcagcgac cggctgaata tgccggttaa aagccaggaa gagattatcc gctcaagcga
     8161 tattccggag gaaatgatgg atcccctcat aagggatttc cgcagcctga tcctctataa
     8221 cctggaaact tccggagctg tgcttacgac cgacaatcag gtgaagatct acacagacgg
     8281 ccaggcgaaa tttgaaggcc ttcgaaccgc tctgcgccgt gccagccgat ttatccacat
     8341 tcagtattat attattaaaa atgacgaggt atttgattcg atcgtgccga ttctggagga
     8401 taaggtgcgt gaaggcgtag aggtgcggat tctttatgac ggcatggggg gtcgctttat
     8461 gccgaaacgc cggtgggagg aactgcgccg cgccgggatt ttagtcggtg tatttttccc
     8521 tcctatttta ggctggctga acctgcgtgt aaattacaga aatcatagaa aaatcgttgt
     8581 gatagacggg aatgtgggat ttgtaggcgg ttttaatatt ggacgtgaat atatttcaaa
     8641 ggatcccgga ttcggttatt ggagagatac ccatttgcgg atcaccggtg aagcagtgat
     8701 gagcctgcag atccgctttg cactggactg gaattatgct acgggagaga atctctttaa
     8761 aaatgaggcc tatttccgga cagatctgaa aaagaagacg gacgtccgaa gggataaaga
     8821 aagaccggcc tggcctgatc ctgaactgga gggaagccag gtgggaatgc agatcattgc
     8881 cagcggtccg gataccagag aaccccatat ccgaaacaac tatctggaac tgtttcacaa
     8941 ggcaaagcac catatttata ttcagacgcc ttattttgtg ccggacgacg ccattttttc
     9001 ggcgcttaag atggctgctt tgtccggggt cgatgtgcgt ctgatgattc cctgtaagcc
     9061 ggatcatccg tttgtgtacc gggcaacctg ttcttatgca ggagaacttt tggattatgg
     9121 tgcaaaggtc tatatctata ctaatggttt tctccattcc aaggggatta tggttgacgg
     9181 ccgggtctgc tgctatggca ctgccaatat ggatatccgg agctttgaac tgaattttga
     9241 ggtaaacgct acgatttatg atgaggcggt cacggcagaa ctggagcagg catttatgaa
     9301 tgacctgcct cactgccgcc agataacaag agaggattat aaaaaaagag gccttctgac
     9361 caggatcagg gagcagggaa gccgtctttt gtctccgctt ttataggaag aataattttc
     9421 agttctgctg tccatactag ccctatgaag agacagtata atggaaaatt aaaagaatcg
     9481 ctccgcaatt tctgccgctg cgggattgcg ggctggtgtc tggaggtcat tttcacttca
     9541 tcggaatcct ggatgcgggg agatatgcgg ctcatggggc gcacctcgct ctggatgttt
     9601 cccatctatg gcatgggagt ccttctgccg gggatcgtaa atacggcaga ccgttggctc
     9661 accggcctgt ctgcattccc ggaggcggga accgatcgta tttcaccacc agccagactg
     9721 gtgcgccacg gacttatgga tatggttctg atttttacgg cagagtatgt gacaggaacg
     9781 attttaaagc attttaatgt gtgcccatgg gattattcca tgtggcccga tcatatcggc
     9841 ggggtgatcc ggctggcttt tgggcccctg tggttttgca ccgggcttct ctttgagatg
     9901 cttcagaccc ccggcagact tgaaaactca aactaacaaa gagttgtatt tccgatgttt
     9961 tctggtatat aatggatacc atcagcgggc aggagagcat ccctttatcc gctgatggca
    10021 gtaaatattt tgaacagaag gagagttaca tatgatacgc ttattgctgg cagccatcgc
    10081 cattgtgctg tatttggtac ttacttttcc ggttttgtgt tttctggaga aaaaggcaaa
    10141 aaagaacccg gaggaggcag gctggaagag cttttccatg gtgcggtgga tttttaaaaa
    10201 aatcagccgg atcgccggga tcacatacga ggtgcggggg ctggaaaata ttcccaccga
    10261 ccgggcggta ctttatgtgg gaaaccacag aagttatttt gatattgtca tgggctatat
    10321 gaccgttcct ggactgaccg gttttattgc caaaaaggaa atgataaaga tcccgctgct
    10381 aaaccgctgg atggatcgga tgaactgctt atttttggat cgttcgaata ttaaagaagg
    10441 tttaaaaact attttggcag gaattgaaaa gatcaaggga ggaatttcca tgtggatttt
    10501 ccccgaagga accagaaatc ccggtgaaaa tccggaaaaa ctgatggagt tccatgaggg
    10561 aagtttaaag attgcagaaa agagcggctg tccggtgatc cctgttgcca tggtggggac
    10621 ggcagaggta tttgagcgtc atttcccatg gatccggccc agccatgtga tcatcgaata
    10681 cggaaagccc atttatttaa aggaacttcc ggcagaaaac cgcaagttcc ccggagccta
    10741 cacaagagag atcatttgct ccatgataaa agagctggag gaacagagat gaaggaactg
    10801 aacttacagg agatccggca gcagctggat gtgattgacg ccagcctgac agagctgttt
    10861 gagacacgta tggagctgtg cagagaggtg gctgagtata agatacagac cggaaagcct
    10921 gtttatgaca gggagcggga gcagcagaag cttaaggcgg ttacggcttt ggctcacagt
    10981 gattttacgc gcaaaggtac atatgaactg ttttcgcaga tcatgaccat cagccgcagg
    11041 cttcagtatg gtcttctggc ttcccacggg caggagatgg acaccgggtt ttccatgatc
    11101 gaggatttaa aaagggaaaa tgtgcggatc gtatatcagg gtgtggaggg ctcctatgga
    11161 cacggtgcgg cccgccggta tttcggcccg gatgcggata tgtaccatgt aaagaccatg
    11221 gaggatgcca tgacagaggt ggaggagggc cgggcagatt atgcggtgct ccctattgaa
    11281 aactcctccg ctggggcagt aagcgacaac tatgatctct tagtaaagca taatgtgtac
    11341 atcgcagcgg aaacggagct tccgattaac catgcgctgc tgggccggga agatgcggat
    11401 attaaggata tccggacagt ttattcccat ccgcaggctc tgatgcagac ctcccagtat
    11461 ctgaatgccc acagggactg gaaacagatc agcacggtca atacggcggt agcggctaaa
    11521 aaagtggcag aggaaaacga tcgttcccag gcggcggtag ccagtgagat tgcgggagag
    11581 ctttatggct taaaggtgct ggagcgctcc attaaccaca ataaaaataa tgtgacccgt
    11641 tttattatcc tgtcaaagga tcctgtatac agaaagggcg ctaataaggt gagcatctgt
    11701 tttgagggcg tccacagaag cggctctctt tacaatatgc ttggaaatct gatttacaat
    11761 gacatcaata tgctgatgat cgaatcgcgc cccattcccg ggagaagctg ggaataccgt
    11821 ttttttgtag atgtggaagg gtctttatcc gatgcaaaga tccgcaatgc tttaaagggg
    11881 atttctgagg aagccgtatc cttaaggatc ctgggaaact actgagccga accggttgga
    11941 gcgtggattc ccttatccgc tgatggtaag ggaaatatgg atacaaagaa gaaacgagga
    12001 aagagatatg gatatcagag gacttgtgat ctaccgcaat ttaaaacatc aaacgctgtt
    12061 tgagcagatg gcaggactta tgggaatgtc agaagaaaag gagagtacag attcttttgc
    12121 ctgtgcggga cagctgatcg aactggctgc aaagtacggc tttgaaggaa acctgtggca
    12181 ctgttttctg gcgctctgcc tggcagataa tgaaaatgca tatacaactg cctgtgaggt
    12241 caaagggcct gcaggcggta ctctggatct tctggcgaaa gaagactttg ccattttcaa
    12301 ggcactgttt gattttgatc tttcttctct tgcgccctct tctttgtgga gcctgcttgc
    12361 ggactatcgc ccggcacttc aggagagccg ggtttttaac cgcaggatca gggacaggat
    12421 cgtggagctg gcaaaggccc tggagcaggc atctgacctg gaaggatttc agcagactat
    12481 ggtggaattt tacaggggat acggggcggg aacttttgga ctgaataagg ctttccggat
    12541 tctggaaaaa gaggccggag ggctgacggt gatcgatccc attgtaaatg tggagcatat
    12601 ttattttaag gatattgtgg gatatgagct tcagaagcat aagctgattg aaaatacgga
    12661 ggcttttgta aatggaaaag aggccaacaa cgtactttta ttcggtgatg cgggaacggg
    12721 aaaatcctcc agcgtaaagg cggttttgaa cgaatattac agcaggggcc tgcgtatgat
    12781 tgaggtatac aagcaccagt ttaaggatct ttcagatgtt ctggagcaga tcaaggaccg
    12841 caattacaag tttatcattt acatggatga tctgtccttt gaggattacg agctggaata
    12901 taagtatttg aaggctattt tggaaggagg actgggaaaa cgcccggata atgtgctgat
    12961 ctatgcgacc tccaaccgcc gccatctcat ccgggagaaa ttcagcgaca agagggagct
    13021 ggatgacgat cttcacaata atgatacggt tcaggagaag ctttctctgg cagcccggtt
    13081 cggcgttacg atttactacg gttcaccgga taaacggcag tttcagtcca tagtcaaagc
    13141 gctggctaag aggcatcagc tggatatacc ggaggaggaa ctgcttttgg aagccaataa
    13201 atgggaattg agccacggag gactttccgg gcgtacggct tcccagttca ttacccatct
    13261 gctgggatgc gattttacat aaaatataca gaaattttac atcataagga agaaagaaga
    13321 tgagtccggg attataatag cagtcatata gtcccgggct taggccatac aggggatcgg
    13381 gcagcgcgcc ccaaaaggag gaagaacgtg gcagtaagaa tgtttgcagc gatagagatc
    13441 ggctcatttg ctctggaact gggaatttat gagatttctg aaaagacggg gatccgttgt
    13501 gtagatcaca tccgccatgt gatcgctctg ggaagcgata ccttcagcga aggaaaaatc
    13561 agctacggtc tggcggatga gatgtgctct gttctggagg attttacggg gattatgaaa
    13621 acctaccggg tgcgggagta ccgggcttac ggaaccacag ccatgcgcga ggcgaaaaac
    13681 agccagatca tcctggatca gatctgtgta agaaccggac ttaaggtgcg aattatcagc
    13741 aattcagagc agcgttttat cagctataag gcagtggcgg caaagacagg agaatttaac
    13801 cggatcatcc agaagggaac ggcgattgta gatgtgggct ttggaagcgc ccagatttct
    13861 ctgtttgata aggatactct ggtaacgacc cagagcctgc ctcttggtac tctgcggatc
    13921 cgcagccagc tggctaaaat cccggccagt gtaaggtcgc attgtcttca cattgaggag
    13981 ctggtagata atgagctgat aacctttcgg aaaatgtatt taaaagacag aaagattgat
    14041 aatctgatcg ggatcggaac caatattacc tatctgatgc gccagcttgg attcaacaca
    14101 gcggcagacc gtgtggatgc caaagagatg gagctttttt atgagcgcct ttctcagatg
    14161 accctggatc agattgagga gcgttttaat gtgaacagtg agtacgcatc cctgctcctt
    14221 ccggcagcgg ttgtataccg cagagtgatg gaggttacgg gagcgggaca gttctggatt
    14281 cctgatatcg gcctttgtga cggcattgcg gcagaatatg ccgatgactg ccgtctgttc
    14341 cgattcagcc acagctttga gaatgatatt ctggcagcgg ccagaaatat ggcaaagcgg
    14401 tataaatgcc atgcgggcca taatcagatg ctggagcagt atgcgttatc tatctttgac
    14461 gctgcgcgga aatttcatgg tatgggagca agggagcggc tcctgctcca gatcgcagtg
    14521 acgctgcatg gctgcggtaa gtttatcagc atccgaaatt ccaatgaaag cagttataac
    14581 ctgatcatgt ctacggagat tatcggactg agccatctgg aacgtgagat tatcgcaaat
    14641 gtagtccgct acaatatccg tgattttgat tacgatacca ttcagctgga aacgcagatc
    14701 ggagaggatg gaagcggcaa tctaaaccga agctccatta ccattcttat agccaagctg
    14761 acggcaatcc tgcgccttgc aaattccatg gacaagaccc acaagtccaa gctggcagac
    14821 tgccggatgg cgatccggga gggagagtta gtcattacca caggatacac gggagatctg
    14881 gctctggagc gggcgtcctt tgaacagaag gcggattttt ttgaggaaat atttggaata
    14941 aggccggtgt taaagcagaa aaggagggca tgacatggca gaaaaagaaa aaaataccac
    15001 agattttgga gcagatccct ccaattttgt taatcgggaa ctgagctggc tggaatttaa
    15061 ttaccgggtt ctcagcgaag ccagagataa gagcctgccc ttgtttgagc ggctgaaatt
    15121 tttaagcatt acagcctcca atctggatga attttatatg gtccgtgtgg cttccctaaa
    15181 ggatatggta cacgcgaaat atatgaagca ggatattgca ggaatgacgg ctcaggagca
    15241 gttagacgct atcagcataa agacccatca gctggtggag ctgcagtatt ccacctatat
    15301 ccgttccctg gtgccgtctt tgcgccagaa cggcctgcgg atcatcatgg agcatgagga
    15361 tcttacaaag gaggaagcgg cctgtgcgga ctcctatttc cagaccaatg tatacccggt
    15421 gcttaccccc atggcctttg attcctcacg gcccttcccg ctgatccgca acaaaaccct
    15481 gaatattgcg gctctgttaa agaaaaagaa cggggatgga gaactggaat ttgccatggt
    15541 gcaggtgcct tctgtgctgc caagaatcgt agagcttccc agagagacaa tggaggatgg
    15601 cacagagcag agggtggtga tccttttaga ggagatcatt gagcggaata tgccctccct
    15661 gttcttaaat tatgatattg tggcctctca tcccttccgc atcatgcgga atgcagatct
    15721 ttccattgat gaggaggagg cggtggatct tctggaggaa atccagaagc agctgaaaaa
    15781 gcgccagtgg ggcgaggcaa tccggctgga gattgaatcc aatgtagata agcggcttct
    15841 taaaatcctg aaaaaggagc tttccatcag cagtcaggat atcttccgca tccctgggcc
    15901 attagatctg accttcctta tgaaaatgta tggtctggaa ggctttgaag cctttaaggc
    15961 tcccaagtac atcccgcagc cggtaccggc cctgatgaat gaggatgata ttttcaccaa
    16021 catccgaaag ggcgatatcc tgctccatca cccatatcag agctttgatc cggtggtgga
    16081 ctttgtgcgc agcgcagcca gagatccgga ggttttagcc atcaaacaga ccctttaccg
    16141 tgtgagcgga aattccccca tcattgcggc tttggcagag gcggcggaga atggaaagca
    16201 ggtatccgta ctggtggaat taaaggcccg ttttgatgag gaaaataata tcaactgggc
    16261 aaaaatgctg gaaaaggccg gctgccatgt gatctacggc ctggtgggac ttaagaccca
    16321 cagcaagatc actctggcag tccgcatgga ggaggacgga atccgccgct acgtccatct
    16381 gggtacggga aattacaatg attccactgc aaagctttat acggactgcg gcattttaac
    16441 ctgcgatccc cagatcggcg aggacgccac ggcagtcttt aacatgctgt ccggatattc
    16501 ggagccaaag gcctggaata agctttctgt cgcgcctctg tggctgagaa accggtttct
    16561 gcgaatgatc cgccgggaga cagcccacgc aaaggaagga cgcagaggcc atattatggc
    16621 aaagatgaac tccctttgcg acaaagaaat catcaaagct ctctatgagg cctcctgtgc
    16681 cggagtggaa attgagcttg tgatccgcgg gatctgctgt ctgcgtgccg gagtgccggg
    16741 gttaagcgag catatccatg tgcgttccat tgtgggaaat ttcctggagc acagccggat
    16801 tttttatttc ctcaatgacg gaagtcctga ggtttatatg ggaagtgcag actggatgcc
    16861 gcggaatctg gaccgccggg tggagattct gttcccggta gaggatgaag ctttaaagga
    16921 gcaggtcatc catattctga aggtagaact ggaagataat gtaaaagccc acattttaca
    16981 gccggacggc acctacgaaa aggaggataa gcggggaaag gtcttaataa attctcagga
    17041 gcagttctgc ctggaggcgg tgcagcgggt aaaggaatac aggggagagg acgacccggc
    17101 agtgacccgg atctttaaac ccatagaaag cggaaatata taaattcaat aaaaagagcg
    17161 gttctgctga tgcaggactg ctttttttat ccgtaacata ttttcccata atttttcaag
    17221 aaaaaagtaa ggaaaaccat tggttaatat ggtatgataa tcataaatat actttacaaa
    17281 gaaatgataa cgtatgagga ggggaatcat atgcccgata tcaacattct ggtagtagac
    17341 gatgaaaagg agatcgcaga tcttatagag atttatctgg taagtgatgg ctataaggtt
    17401 tttaaggcgg aaaatgcctt aaagggcctt gagatcctgg aaaaagagga gatccatctg
    17461 gtgctccttg atatcatgat gccggggatg aacggccttg agatgtgtaa aaagatccgt
    17521 gaaactaaca atatcccgat cattatgctc agcgccaagt caaccgacct tgacaagatc
    17581 ctgggactgg ggaccggagc ggatgactat gtggtaaagc cctttaatcc attagaactg
    17641 acggcacggg taaaatccca gcttcgccgc tatacccagc taaatcccaa cagcatggca
    17701 aaggaagcgg taagcagcga aatcaccatc aagggaatga gcatcaacaa agataatcat
    17761 aaggtagtgg tagacggtga ggaaatcaag ctgacgccga ttgaatttga cattctttat
    17821 cttctggcct ccaacccggg aaaggtattc agtacagatg agatttttga gcgggtatgg
    17881 aatgaaaagg tatatgaggc caataatacg gttatggtac atatcagacg tttaagagga
    17941 aagatgaagg aagataccag gcagagcaag attattacaa ctgtgtgggg agtaggctac
    18001 aagattgaaa aatagtatgg gaagcagtta ccggttccgt gtgctgaccc atatcgtgta
    18061 cagcgccatt attacctgcc tggtagaagt atttcttatt acaaatcttt ccatggtggc
    18121 gcgctacata cagcagagcg ggaaggtatc ggctctggtc cattttattt tagagcagca
    18181 tctggcagtg gtgatcctgt atgtgctggt gggaattgtg atcttttcgg tgacctttat
    18241 gattttggag gagccgggga tccgctatct gggaaggatt tcggatgcgg ttcagagcat
    18301 ttcccagggc aatttaaata cggaagtgga tgtgaccggg gacgacgaat tttccgccat
    18361 ggcggcaaat ttaaacaaga tgtcctcaga tatccgaaag cttatggata aggagagaga
    18421 agcggaacgc acgaaaaatg aactgattac aaatgtggct cacgatttaa gaacgcccct
    18481 tacctccatc atcggctatc tggagctttt ggccgggaat acgcagatcc cccaggaaat
    18541 gcagcacaag tacatagaaa ttgcctacag caaatccaga cggctggaaa agctgattga
    18601 agaccttttc ggttttacga agctgaatta cggaaaaatc gccatgcaca taggacagat
    18661 cgatattgta aagcttttag agcagctctt agaggaagcc taccccaact ttgaggaaaa
    18721 aaatctgtcc tatgaccttc agagcaatgt gcctgcaaag atcataagtg ctgacggaaa
    18781 tcttctggcc cgtctgtttg acaatctgat tggaaatgcc attaagtacg gagctgatgg
    18841 aaagcgggtt ctggtaaaga tccacggaga ggaggacacg gttacggttt ccgtgacgaa
    18901 ttttggccgt gtgatccctg cagatgagct gcctctttta tttaacaagt tttaccgggt
    18961 ggagcagtcg cgttctgcca caaccggagg caccggcctt ggtcttgcga ttgcaaagga
    19021 aattgtggat atgcacggag gcaccatccg ggttgccagc gatttaaacg gtacggtatt
    19081 tacggtaaag cttcaggtac attttgatct tgagaaagaa cagttcggat catgacggtg
    19141 atccggccat aaaaagagag gaaaatacag atgtacagat atatacgggt gctgaaaggc
    19201 gccctttttg cccttttgtt ttctctttcc gccctgctcc cttcctatgc tgcaattacg
    19261 gcagacggtc cgggaagcgg tgtggcaggc cccagagaga ctgtgccgga agaaggcgca
    19321 cagacagagg cgctttggca ggatccggct tcaccccatc cattggagat ggaagtggtg
    19381 tacggctatg ataacacagc cagaagcggc cgtatccttc cgatgaccat ccgggttaca
    19441 aattctacaa aggaggactt tacgggaacg cttcgggctg caactcttga accggaatac
    19501 agtccttatg gaaatagttc tctttcagag tacagtacct accggtatga ataccccatt
    19561 gaggtaaagg caggggaaac ggttgaaaaa aagattcaga tttccctcag cattcaggta
    19621 gatcaggtac tgctgtcttt agtagatcag gaaggaaagg agacagccgg acgccggatc
    19681 aggctggatc tgaacctgga tacagcggag ctgtatgtgg gggttttgag cgaccatccg
    19741 gaacgccttt cctatatgga tcatatgggg atcaattaca gtacactccg cacccgtctt
    19801 attgagctgg atcaggatac gatgccagaa tcccggcagg agctggatca gctggatgtg
    19861 cttttgatca atgattttga tactgggtct ctggacagcc ggcagcttga ggctgtatgg
    19921 gagtggaccc agagcggagg tacccttctg ttcggaacag gggagcatgc aaatgaaacg
    19981 ctgggagcct tttccaaggc gctcttagcc agcccacttc ctcaggccgt agaatttgaa
    20041 ctggatatgg aaagcagtca ggaatcctcg gaatatgggg acagccggat tgttctgaac
    20101 tgcacagagg tggacttaaa gggaagtacg gaggtcatga caaaggggcc gatggaaatt
    20161 atttccagca caccggtggg cagaggaatg gcggcagcgg caatttatga ttttggagat
    20221 ctgaaagaat acgggactga caatccctcc tatgtagacc gcctgtttac agggcttata
    20281 ggagaaaacc gtctgaatct tttaagcaac ggaaacggag gctcctccac aggccgttac
    20341 tgggaggtac agagcctgat caatacgggg aatgtaaacc gtcttcccaa agtagggctt
    20401 tacgttacgc tggcagttgc ctacgtggct cttgcggggc cggggcttta cttcttttta
    20461 aagcagaggg agctgcgcca gtattatcag cctgcagtag ccattttgtc cctgtgctgt
    20521 accggtatgg tgcttttgat ggggataccc acgcggttta acggcccgtt ttttacctat
    20581 gcctccattc aggacgcagg agaagaggat gtatcagaaa cgttgtttat caatatgcgc
    20641 tctccctata atcacagcta tggggtatct ctggatcctt cgtaccgtct gttccctgtc
    20701 tgcgattcct cgtactacaa tccgggtacg gttccggctc tggtggattc tgaggatccg
    20761 gatatggtga tccggtataa ggaggatgaa acgtatattg aagcgagaga tacaggcgct
    20821 ttcaatccca gttatttcca gatggagcgt aacctgccaa atgaaacagg gcagggattt
    20881 tccggggaga tacgggcctt tgagggaacg atcacaggga cgataacgaa taattacccg
    20941 tggaccgtag aaaatgcggc gctgctttta tataaccaga tggtaatgat cggaaccata
    21001 gagcctggac agacgatttc cctggatgga cgcgagctga tatactgcgc aacggattta
    21061 ggatatgcta tggccgctca gattaccgga gcttcacggt acggccagaa ggtaaatata
    21121 gaggatccgg attatgtaag ggcgctggag cggaccaatc tcctttcctt ctatgtggaa
    21181 aattattttt ccggatatca tacccaggca cgggtggtag ccttcagcca ggagcagaag
    21241 gaaacgggat ttttgagaaa tccgggaaca gaaacctatg gctgcacgct tttaacttcg
    21301 gagttggatg taaattatga acaggatggc cttgtaagcc gttcggcgat gcagaagcag
    21361 ccgcatgtgc tggcaggaga gtacgatgcg gcgcgcaaca ccatctacgg gataaatccg
    21421 gtggttctgg aatattatct tggaaatgag ctggaagtag atacgcttca ttttcatcgt
    21481 ctcagcgaag gagtggttgc aaatttaaga tattattata ccgtaccgtt tgaaggaaat
    21541 atgtattttt acaactataa tacaggctct tatgatcgga tggacagtgc ggtaagccag
    21601 tataacaggg aggaactgga ttcctatttg tcacctggaa atacgatcac tgtgaaatat
    21661 gtgtatgata cagccggaga gtatacatgg aatatcatgc tcccggttct tacagttaca
    21721 ggaaggagac agccctgatg cttacagtaa gaaatcttca caaaatatat ggaaaatacc
    21781 atgccttaag cggcctggat atgaatgtag gcgatggcgc actttacggc tttgtaggac
    21841 ccaacggcgc aggaaagaca acgaccttaa aaattatgac agggcttttg ggcgcagaag
    21901 aaggaagtgt gaccattaat ggaaaggatg cactcaggga tctgggggaa ttaaagggga
    21961 tgatcggtta tgtaccggat ttctttgggg tatacgataa cctgactgtg gcagaataca
    22021 tgagtttttt tgcctcctgt tatcagcttg acggcctaat ggcaagaaaa cggtatacgg
    22081 ctcttctgga gcaggtagga ctggaggata agctggattt ctatgtggac ggactttcac
    22141 gcggaatgaa gcagcggctc tgtcttgcaa gggctctgat ccacgatccg gcgatcctca
    22201 tcctggatga gccgacctca ggcctggatc cgagaaccag gtttgaattt aaagaaatac
    22261 tcagtgaatt aaggagtacg ggaaaaacca ttattatcag ttcacatgta ctgtcagagc
    22321 tggcggagat gtgtacggat atcgggatca tcgatcaggg acggatggtg ctggagggaa
    22381 acataacggc gatcctgtcg cgggtcaacg cctcaaatcc tctggtaatt tccgtataca
    22441 gcaatatgga gcaggccatg gcggtcctgc gccgccatcc ctgtgtccag actatttcca
    22501 tgcgggaaaa ggatatctgc gtccgctttg ccggagacgg caccgatgag gcgatgctcc
    22561 ttcagcagct gatcgatgct ggcgtcctgg taaatggctt tgttcgtgag aagggcagtc
    22621 tggagtccgt ctttatgcag ctgacagatc atgaagaaga aagggtggtg acgtcttatg
    22681 aggcagaatc cggtttacag ccgtgagatg aaagtcagcg ccagaagccc tagatttcct
    22741 ttgatccttt ccctttttaa cgggattctg tgtctggcgg ctctgatcaa tatgtactcg
    22801 gtggttctgc gggt
//
LOCUS       DBMDUH010000024        22588 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_1485_length_22588_cov_7.494463, whole genome shotgun sequence.
ACCESSION   DBMDUH010000024 DBMDUH010000000
VERSION     DBMDUH010000024.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 22588)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 22588)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22588
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1485_length_22588_cov_7.494463"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1370)
                     /locus_tag="ACZPCG_03630"
     CDS             complement(<1..1370)
                     /locus_tag="ACZPCG_03630"
                     /EC_number="4.1.1.112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015554842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit alpha"
                     /protein_id="HIR1284197.1"
                     /translation="MAEIEKKPVKIVETVLRDAHQSLLATRMSTEQMLPIIDKMDKVG
                     YHAVECWGGATFDACLRFLKEDPWERLRKLRDGFKNTKLQMLFRGQNILGYNHYADDV
                     VEYFVQKSIANGIDIIRIFDCLNDIRNLETAVKATKKEKGHAQIALCYTLGDAYTLDY
                     WRDIARKIEDMGADSLCIKDMAGLLTPYQAAELVQALKEGTKLPIDLHTHYTSGVASM
                     TYMKAVEAGCEIIDCAMSPLALGTSQPATEVMVETFRGTPYDTGYDQNLLAEIAAYFQ
                     PIREEALKSGLLNPKVLGVNIKTLQYQVPGGMLSNLVSQLKEAGQEDKYQQVLEEIPR
                     VRKDFGEPPLVTPSSQIVGTQAVLNVLMGERYKMVPKESKKIMLGEFGQTVKPFNPEV
                     QKKIIGDETPITCRPADLIQPQLPQFEKECAQWKQQDEDVLSYALFPAVAKEFFQYRE
                     AQQKKV"
     gene            complement(1411..2556)
                     /locus_tag="ACZPCG_03635"
     CDS             complement(1411..2556)
                     /locus_tag="ACZPCG_03635"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HIR1284198.1"
                     /translation="MDFGNLVTNLLGQMAFFHLTIGNYVMIGVALVFLYLAIRRGFEP
                     LLLIPIAFGMLLVNIYPEIMYSPEETSNGIGGLLWYFFTLDEWSILPSLIFLGVGAMT
                     DFGPLIANPISFLMGAAAQLGIYAAYFLAIFLGFSGKEAAAISIIGGADGPTSLFLCS
                     KLGQTQIMGPIAVAAYSYMALVPIIQPPFMKLLTTDKERKIKMEQLRSVSKLEKILFP
                     IIVTIVVCLILPSTAPLVGMLMLGNLFRESGVVKQLAETASNALMYIVVILLGTSVGA
                     TTSAEAFLNVTTIKIVLLGLVAFIFGTVGGILLGKLLCKLTGGKINPLIGSAGVSAVP
                     MAARVSQKVGSEYDPTNFLLMHAMGPNVAGVIGTAVAAGTFMAIFGV"
     gene            complement(2607..2993)
                     /locus_tag="ACZPCG_03640"
     CDS             complement(2607..2993)
                     /locus_tag="ACZPCG_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HIR1284199.1"
                     /translation="MKTYTITVNGKAYAVTVEEGAAAPAAFAAAPAPAAPVPAPAPAA
                     APAPAPAAPAGAAGSVKVTAPMPGKIVAVKAAAGQAVKKGETVLVLEAMKMENDIVAP
                     QDGTVASINVSTGDSVESGAVLATLN"
     gene            complement(3026..3853)
                     /locus_tag="ACZPCG_03645"
     CDS             complement(3026..3853)
                     /locus_tag="ACZPCG_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003524245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HIR1284200.1"
                     /translation="MRRLFKRMAAAVLVSACLVSLSACSKTEEETESLSISMDGTPVE
                     DAMGQSLLLSAAQTLGASDEQLVVQGKLAQSQGDTVSAELYQAQLDVREEMGALRSVD
                     IEDGSVVLLEDGSYTVILPVEFAEGTKKYVMNLNMATQQIQAQFTDMSAGVEEDSSIG
                     GLLRTASVYTLIGLGTVFCVLVFISLLISCFKFIHAWEEGQKNKAADKKAEPAKAPVP
                     AVSAAPAVSALQTTGPELADDAELVAVMAAAIAAYEGTSPNGLVVRSIRRVSSSRRR"
     gene            complement(3871..5322)
                     /locus_tag="ACZPCG_03650"
     CDS             complement(3871..5322)
                     /locus_tag="ACZPCG_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HIR1284201.1"
                     /translation="MSNSAQTSASSRIASLLDENSFVEVGAYITARNTDFNMAEQETP
                     ADGVITGYGTIGGCLVYVYSQDASVLGGSMGEMHAKKICSIYSMAMKMGAPVIGLVDC
                     AGLRLQEATDALDGFGKLYLSQAMASGVIPQIMAVFGTCGGGMAVAAGMADFTFMEEK
                     SAQLFVNAPNALEGNYKAKCDTAAAAFQSKESGVVDFTGDEASILSQIRTLVSILPAN
                     NEEDLSEEDCQDDLNRMCSGIEGLAGDPIQVLSMISDNHFVMEVKKDYEPSMVTAFIR
                     LNGVTVGCVANRTERYEDGKKADEFEAALSGKGCDKAVELISFCDAFSIPLLTLTNVN
                     GYKAKMCSERRIARAAARLTYAYADATVPKVTVVTGKALGSAGLTMGSKSLGTDLVYA
                     WPNAVIGTMEPEAAVKIMYAKEIEASDDAVALISEKTAEYTKTQSGAVAAARRGYADD
                     IIKAEETRQRLAAAFDMLYTKSEDRPSKKHGTV"
     gene            complement(5653..6864)
                     /locus_tag="ACZPCG_03655"
     CDS             complement(5653..6864)
                     /locus_tag="ACZPCG_03655"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007717006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HIR1284202.1"
                     /translation="MSLIVQKFGGTSVADTERLRNVARIITETYKAGNQVVAVLSAQG
                     DTTDDLIQKAEEINPKASAREMDMLLSTGEQISVSLCAMAIEALGYPVISLTGWQSGI
                     STNTVSGDARIRKVDTERIEAELNQKKIVIVTGFQGIDRNQNITTLGRGGSDTSAVAL
                     AAVLGADLCQIYTDVDGVYTADPRKVAGARKLDEVTYNEMLELATLGAQVLHNRSVEL
                     ARKYNVKMEVLSSFTGHPGTKVKGVAKRMERSYISSVAKDKDIARIALIGVPNEIGTS
                     FKVFSLLARNHVNVDIILQGIGHEEGKDICFTVAEKDLNKTAELLTEHKEELRYSRME
                     TNCDVAKVSVVGAGMINNPGVAAKLFEALYDAGININMISTSEIKISVLVDRRDADQA
                     VQAIHDRFFTV"
     gene            complement(7319..8143)
                     /locus_tag="ACZPCG_03660"
     CDS             complement(7319..8143)
                     /locus_tag="ACZPCG_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009535534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1284203.1"
                     /translation="MKNKVKILDAASMVLLLLLSVVFIFPFYWILTGAFKSQKVTIKL
                     PPEWFPLHPTLENFEKLFQNPALQWLINSIFIAGVTLIIVCVAASMAGYVLAKKRFYG
                     DKLLFSIFVFAMALPKQVILVPLMKLVSFMGIHDTPWAVILPLCGWPFGIFLMKQFSE
                     NVPTELLEAAKIDGSGEIRTFTSIVMPIVKPGLGALAIFTFINTWNDYFMQLVMLNSR
                     TNLTISLGVATLQQEFSTNYGVIMAGAALAAVPIVAVFLMFQNYFTQGITMGAVKG"
     gene            complement(8158..9054)
                     /locus_tag="ACZPCG_03665"
     CDS             complement(8158..9054)
                     /locus_tag="ACZPCG_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008976623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1284204.1"
                     /translation="MKSRKINKIRMRETLVSYAFLLPAMLFFVLFVLVPMGMGVVTSF
                     FRYNMKGMTFTGLSNYMTMFADPVWQKSLVNTLLLVVGSVPITVLFALFVAAMTYEKN
                     PFTRSFFRCVFFLPVVTGTVAVTVVWKWIYDPLNGILNWILKSLDLIEKNIMWTGDKR
                     YALWAILIILITTSVGQPIILYIASLGNVPKDYVEAAQVDGANDFQVFWKIKWPSLLP
                     TTLYIVVITTINSFQCFSLIQLLTSGGPNYRTTTIMYYLYETAFKKFEYGYANAMGVI
                     LALIIGFISFVQFKALGSDVEY"
     gene            complement(9121..10512)
                     /locus_tag="ACZPCG_03670"
     CDS             complement(9121..10512)
                     /locus_tag="ACZPCG_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016441612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1284205.1"
                     /translation="MKKIVSLGLAAAMAASVLTGCGGGSAQTADTKEDAKTEEAKGSE
                     AASDKAEGTATAEGMTEITWWAFPTFTQDGDNPAGTYEQEIIDAFEAKNPDIKVKLET
                     IDFTSGPEKITAAIEAGTVCDVLFDAPGRIISYAQNGKLVALDDMFTEEFVKDVDNEA
                     LINSCKSGDSYYMYPISSAAFCMAVSKNALEEAGAMDCINLEGDRTWTTEQFEEMMKK
                     LSEAGFMGGTVYCSGQGGDQGIRAFVTNLYDSSVTDPEITTYTLNDEGGVKAMTKIKE
                     WVDNGYMLNGSAYNGGEDVDQFVAGNTAFTTLWSPGLASQRAETLKENGIEAIALPFP
                     SEDGTPALEYLVNGFCVFDNGDEARAEASKKFIDFICNDEEWGPKNVVKTSVFPVRQS
                     MGDLYEGNEDMSFYASLTKYYSPYYNTIPGFAEMRTYWFPMLQAVINGDSEPKAALDD
                     FVAKANQSIVNAQ"
     gene            complement(10785..11708)
                     /locus_tag="ACZPCG_03675"
     CDS             complement(10785..11708)
                     /locus_tag="ACZPCG_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HIR1284206.1"
                     /translation="MRDITKYQGIIPAFYACYEEDGSISTERTKALAEHLLKKGVKGV
                     YVGGSSGECIYQSKEERKAVLEAVMETVKGKMTVIAHVACNNTEDSCELAAHAESLGV
                     DAVASIPPIYFHLPEHAIAQYWNDISAAAPNTDFVIYNIPQLAGVALTMPLFREMCKN
                     PRVAAVKNSSMPVQDIQMFKMEGGKDFVVFNGPDEQLVSGLAMGADGGIGGTYGVMPE
                     LYLKIFELCRKGKYEAARKIQYDADAIIYAMCACHGNLYAVMKEILKLREGLDLGSVR
                     KPMPGLIPEDMEQVKKCAAMIDAAIAALQEI"
     gene            11862..12503
                     /locus_tag="ACZPCG_03680"
     CDS             11862..12503
                     /locus_tag="ACZPCG_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HIR1284207.1"
                     /translation="MKKHIVCFGDSNTHGYCAETGGRFSEKERWTCLLQELLGTEYLV
                     LEEGLSGRTCCFPDPLYEGLNGLDSIYPCLMSHEPVDLLVIMLGTNDTKERFGASSAC
                     IALGLKRLIEKARSISDCWRNGPNILIVTPKNIDPEYAGSSVGNTMGRGCAEKSAGLG
                     SEYEKIALLTGCRYFDANTVVTENNHIDFMHLTAQGHRELAEALAKQIPCLIP"
     gene            complement(12583..13440)
                     /locus_tag="ACZPCG_03685"
     CDS             complement(12583..13440)
                     /locus_tag="ACZPCG_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HIR1284208.1"
                     /translation="MQGDFITSIKSAYNQFTKAEKKVADYILANPRDVLFMSITDLAE
                     ACEVGDTSVFRFCKTMDLKGYQEFKMMLSLSISKGQEQVDQFTGNNISMEDSFGELAR
                     KVLNTNINALNETFSMIKEEDIDRAITLIHRARRVFFFGVGASMLTAMKAMNKFMRIE
                     NKVFCIQDSHMQAMAAATMDERDTAVIFSYSGATKDTIHVAEVAKRSGAKIICITRFV
                     KSPLSAYSDVTLLSGANEGPLQGGSTSAEISQLFLVDLIYMEYYRRYFEQCSVNNEKT
                     SGSVLEKLC"
     gene            complement(13536..14441)
                     /locus_tag="ACZPCG_03690"
     CDS             complement(13536..14441)
                     /locus_tag="ACZPCG_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016360066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR1284209.1"
                     /translation="MGTAALDIGGTAIKSGIWTGIKDEIKEVRETPTNARLGGAHVVE
                     QAVKILESYHGFDAIGISTAGQVDSQRGMIRYANENIPGYTGMEIGRMLRERFHVPVA
                     VENDVNAAALGEGIFGAGRGERDFLCLTYGTGVGGAIVMDQKVYHGCAFSAGEFGGLL
                     IHPEDRKEGEFFSGCYEKYASATALAARAMVIDPALTDGRKIFVAIDQPGVQAAVDEW
                     IDEIVNGLQTLIHIFNPSCVILGGGVMAQPYVTEQVRVRTMERIMPSFRDVNILPAEL
                     GNRAGLLGAAWLGREEKRKENGKLQ"
     gene            complement(14452..15150)
                     /locus_tag="ACZPCG_03695"
     CDS             complement(14452..15150)
                     /locus_tag="ACZPCG_03695"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016279108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047465 - N-acylglucosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HIR1284210.1"
                     /translation="MNKQELFEQIKGQMIISCQAIPGEPLYVEEKSVMYLMARAAKMA
                     GTPAIRTSSIRDVAAIKEETGLPVIGLVKIQYPGFESYITPTMKEVDELVAVGSDVIA
                     LDCTLRARGDGKTVNEFIKEVRQKYPDAILMADISNYEEGINAWKLGMDIVGTTMSGY
                     TADSPHMDGPDYELVSRLSKTVDVPVIGEGKIHYPDQAVKMLDAGAFAIVVGGAITRP
                     LEIANRFMDAVKNR"
     gene            complement(15368..16141)
                     /locus_tag="ACZPCG_03700"
     CDS             complement(15368..16141)
                     /locus_tag="ACZPCG_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009250375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HIR1284211.1"
                     /translation="MYRISNFTDNDDVKVLAKLGAFEVIEYQRDLSVTPGSAVTAYYC
                     AQMNVRKRQLVCSLDQSNVTIQAGAMQWMLGDVKATTGIKGVGDFLGKAVRGKATGES
                     AIKPEYTGSGVLVLEPTYKYLILMDAAQWGGGVVLDDGLFLACESTLQHKAVMRSNFS
                     SAVAGGEGLFNLSLNGNGVFCIESECPKEELIEIELQNDVLKVDGNYAIAWSKSLDFT
                     VERSGKSLIGSAASGEGLVNVYRGTGKVLMMPTAKMPNI"
     gene            complement(16196..17932)
                     /locus_tag="ACZPCG_03705"
     CDS             complement(16196..17932)
                     /locus_tag="ACZPCG_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002593143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5722 domain-containing protein"
                     /protein_id="HIR1284212.1"
                     /translation="MKHKLFKRSRGMALAAALFFGVTAVSLGAFSRSVSAEETQASGE
                     AAQEAAKEDIALTGQIRSCKVTADKQNVEIAFSTSGDTAGTDGKVYIFEQQTYENSLD
                     GRTDYIASADALISSSAQVPLNFGSTADRLYSKFVLAAYDGTEYKAISEPHYITNPEA
                     VAKNTEAFKDPLTKKGLNIEIDMLEDAFDLGVKHVTTNIAFSQIMGTGIEYEYDGKIY
                     HFNKAIMDDYDRTISALSNKGMTVTVIILNDWNPNRSDLIYPGTKKSSNAFYYMFNGA
                     NEAGFEQTRAIAAFLADHYSGKDSNHGKVSNWIIGNEINNQQWNYMGSMDLTNYVKVY
                     QQAFRIFYTAIKSTNANDRVYFSLDYNWMNEIDGKLKYGGKEIVDSFNSIANVQGQMD
                     WGLAYHPYPCPMIEPEFWDDPKATGLFTNDFNSPVINFANLNVLTDYFNQEALKAPSG
                     NVRHIILTEQGFTSYSPTRGDIPEIQAAAYAYSYYLVDSNPYIDAYTVSRQVDAPSEA
                     KQGLKFGLWECDMNQPDKIVATKRKKIWQVFRDIDKKNSTLETSEFAKSIIGIEKWSD
                     VIPNFKWKNLEK"
     gene            complement(18053..18931)
                     /locus_tag="ACZPCG_03710"
     CDS             complement(18053..18931)
                     /locus_tag="ACZPCG_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007717014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIR1284213.1"
                     /translation="MEQKNDRQKWLRMTARVGAILFGNAIYAMAVVLFIMPNGLVTGG
                     TTGMGLFFNRLWGIPVSLFVSIFNIAVFGLGLWQLGKEFALTTALSTVFYPAALAVME
                     RTGIHGFYMEERLVAVIYAGILIGAGIGIVIKAGASTGGVDIPCLILRKKCGINVSLS
                     LYVLDCVILAMQLITSSAEAVLYGILLIVTYTLVLDKVLMAGMTKMQVKIVSRRHEQI
                     NALISQRLDCGTSLLHMETGYLHREQDMVLAVISKRDLPRLNQLVLDEDPEAFMIINQ
                     INEVQGRGFTLKKVYK"
     gene            19329..20045
                     /gene="thiW"
                     /locus_tag="ACZPCG_03715"
     CDS             19329..20045
                     /gene="thiW"
                     /locus_tag="ACZPCG_03715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HIR1284214.1"
                     /translation="MNKNKHAAKLVFLAMMTALGVVISPILRVEGMCPMAHFINVTCA
                     VMLGPWYAFVCALAIGIIRMTCMGIPPLALTGAIFGAFLSGMLYRMSRGRLVWAFAGE
                     VIGTGIIGAILSYPVMTFVWGKTGLTWFFYVPSFIAGTLIGGTIAFLFLKHLQKARLL
                     SMFQETLGSRTYDSGEDVVNDALGIAFSGFIGYLAVTVAVHQLVPQGGSVINSLRYIV
                     LVGFLAAALIYWSIKRPKAA"
     gene            20094..20912
                     /gene="thiM"
                     /locus_tag="ACZPCG_03720"
     CDS             20094..20912
                     /gene="thiM"
                     /locus_tag="ACZPCG_03720"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: protein motif:HMM:NF006830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HIR1284215.1"
                     /translation="MNPISLLHKEAPLIHCITHPIVVNDCANAVLALGARPIMAEHPK
                     EAASIAQIARSLTVSLGNITDARAESIFLAGNVRPAVIDLVGITCSDFRMKLARRFLE
                     ECRPAVIKGNVSEIRAIAGAGFHNQGIDVSREDAVTKNDPMAQFRLARLMKEIADRTQ
                     AVVAASGEVDIIVSPQDDKAYFLENGSPSMARITGTGCMLTCIMGTFMAVVSPLEAAV
                     CGAAVLGIAGERADASKGLGTYHISLLDQLSLMTDETLKSEIRLHSVDLSSTAS"
     gene            complement(20899..21513)
                     /locus_tag="ACZPCG_03725"
     CDS             complement(20899..21513)
                     /locus_tag="ACZPCG_03725"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF014622.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009229 - thiamine diphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HIR1284216.1"
                     /translation="MYERMVAVTNHRLCRVPEGADQTEDGCFEPGTGLWESYLDQIRK
                     IVSVKPRAVVLREKDLSPEIYRKLAEEVDQICREQDRMLILNTAPAAAAQLGMRKLHL
                     PMSILRREGRPLDAECVGTSIHSLEEVKEAVRLGADYLFAGNIYETDCKKGLPGRGLD
                     FLSQVCMESSVPVYAIGGVNEERLPEILSTGAAGGCMMSGFMKL"
     gene            complement(21644..>22588)
                     /gene="tuf"
                     /locus_tag="ACZPCG_03730"
     CDS             complement(21644..>22588)
                     /gene="tuf"
                     /locus_tag="ACZPCG_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006568224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HIR1284217.1"
                     /translation="PGHADYVKNMITGAAQMDGAILVVAATDGVMAQTREHILLSRQV
                     GVPYIVVFMNKCDMVDDAELLELVDMEIRELLNEYEFPGDDTPIIQGSALKALEDPMG
                     PWGDKVMELMDAVDSYIPDPVRETEKPFLMPVEDVFTITGRGTVATGRVERGTLNLND
                     EVEIIGIHEDVRKTVVTGIEMFRKLLDSAEAGDNIGALLRGVQRTEIERGQCLCKPGS
                     VKCHNKFTAQVYVLTKDEGGRHTPFFNNYRPQFYFRTTDVTGVCELPAGTEMCMPGDN
                     VEMSVELIHPVAMEQGLRFAIREGGRTVGSGRVVSIVE"
BASE COUNT         5533 a         6122 c         4823 g         6110 t
ORIGIN      
        1 tctacctttt tctgctgtgc ttctctgtac tggaagaact ccttggctac agccgggaac
       61 agagcgtagc ttaatacgtc ctcatcctgc tgcttccact gagcgcattc cttctcaaac
      121 tgaggaagct gaggctggat cagatctgcc ggacggcaag tgatcggcgt ctcgtcaccg
      181 atgatcttct tctgtacttc cggattaaat ggctttacag tctgtccgaa ctcaccaagc
      241 atgatcttct tggactcctt cggaaccatc ttatagcgct cacccataag cacgttgaga
      301 actgcctgtg taccaacgat ctgagaagaa ggagttacca gaggtggctc tccgaagtct
      361 tttcttactc ttggaatctc ctctaatacc tgctgatact tgtcttcctg tcctgcttcc
      421 tttaactggc ttacaaggtt ggaaagcatg ccgccaggta cctgatactg caaggtcttg
      481 atatttacac ccagaacctt cggatttaag aggccgctct ttaaagcttc ctcacgaatc
      541 ggctggaagt atgccgcaat ttccgccaga agattctgat catagcctgt atcatatgga
      601 gtgcctctga aggtctcaac cataacctca gttgccggct ggctggttcc cagagccaga
      661 ggtgacatag cacagtcgat aatctcacag cctgcctcta cggccttcat ataggtcatg
      721 gaagcaacgc cggatgtata atgggtatgc aggtcaatcg gaagctttgt gccttccttt
      781 aatgcctgta ccagctctgc cgcctgatat ggagtcagaa gacctgccat atccttgata
      841 cacagggagt cagcacccat atcctcgatc tttcttgcaa tgtctctcca gtaatccaga
      901 gtataggcgt cgcccagggt atagcacaga gcaatctgag catgtccctt ttccttcttg
      961 gtagccttta cggctgtctc cagattgcgg atatcattta aacagtcaaa aatacggata
     1021 atatcaatac catttgcaat ggacttctgt acgaaatact ctactacatc atctgcataa
     1081 tggttgtagc ccaggatatt ctgtccgcgg aacagcatct gcagctttgt attcttaaag
     1141 ccgtctctta attttcttaa acgctcccat ggatcttcct ttaagaaacg caggcaggca
     1201 tcaaaggtag cgcctcccca gcactctact gcatggtagc ctactttgtc catcttatcg
     1261 atgatgggaa gcatctgctc tgttgacatt ctggtcgcaa gcaaagactg atgggcgtca
     1321 cgcaggacgg tttccacgat ctttaccggc ttcttctcta tctctgccat agttaaaaat
     1381 cctccttaaa tactgtgata aaattccctt ttatacgcca aagattgcca taaaagtacc
     1441 ggctgctaca gccgtgccga tcacgcctgc aacgtttggt cccattgcgt gcatcagcag
     1501 gaagttggta ggatcatact cggaaccaac cttctgggat acgcgggctg ccataggtac
     1561 ggcagacacg cctgcggaac cgataagagg attgatcttt cctccggtca gcttgcacag
     1621 aagctttcct aacaggatac cgcctacggt accgaaaata aatgcaacca gacctaaaag
     1681 aacgatcttg atggtagtta catttaagaa tgcctctgca ctggtagtag cacctacgga
     1741 tgtacccagc aggataacga caatgtacat cagggcattg gatgctgtct ctgccagctg
     1801 ttttacaaca ccgctctcac ggaacaggtt tcctaacatc agcataccta ctaatggagc
     1861 agtagacggc agaatcaggc agactacaat tgttacaatg atcgggaata aaatcttttc
     1921 cagcttggat acggaacgga gctgttccat cttgatcttg cgctccttat ccgtagtgag
     1981 cagcttcata aacggcggct ggataatagg aaccagagcc atataggaat aagctgcaac
     2041 tgcgattgga cccatgatct gggtctgtcc cagcttactg caaaggaaca gagaggtagg
     2101 gccgtcagca ccgccgatga tggaaatagc agccgcttcc tttcctgaga atcccaggaa
     2161 aattgccagg aaatatgcgg catagatacc cagctgagca gcagcaccca taaggaagct
     2221 gatggggttt gcgatcagcg gaccgaagtc tgtcatggct cctacgccaa ggaagatcag
     2281 ggacggaagg atactccact catccagagt aaagaaatac cacaaaagac cgccgatgcc
     2341 gttggacgtt tcttccggag aatacatgat ttccggatag atatttacaa gcagcatgcc
     2401 aaatgcgatg gggatcagca gcagaggctc aaatccccgg cggattgcca ggtataagaa
     2461 gaccagcgcc acaccgatca tcacatagtt cccaatggtc agatggaaaa acgccatctg
     2521 tcccaaaagg tttgttacta aattgccaaa atccataatg ttacctccat aatggaatcc
     2581 aacttactgc ggatccgtgt ttctgcttag ttcagagtgg ctaaaactgc gccggactca
     2641 acggaatcgc ctgtggatac atttatgctg gcaacggtac cgtcctgagg agcaacgatg
     2701 tcattttcca tcttcatagc ctcaagaaca agaacagttt cgcctttctt tactgcctgg
     2761 cctgctgcag cctttacagc tacgattttt ccaggcatag gagccgttac cttaacggag
     2821 cctgctgcgc ctgcaggtgc agcaggagct ggtgccggtg cggcagcggg agctggtgcc
     2881 ggtacggggg ctgcgggagc cggtgcggct gcaaaagcgg ctggtgcggc tgcgccttct
     2941 tcaacggtta ctgcatatgc ttttccattt actgtaatgg tatatgtctt catgatctgt
     3001 ttcctccttg atttgaaatg agtctttatc ttcttctgga actgcttacg cggcggatgg
     3061 agcgtaccac cagaccgttg ggtgaagttc cctcataggc cgcaatcgct gctgccatca
     3121 ctgctacaag ctctgcatca tctgccagct ccggtcctgt tgtctgtaaa gcagaaactg
     3181 ccggtgcagc agaaacagcc ggtacaggag ctttcgcagg ttctgccttc ttatcagccg
     3241 ctttattctt ctgtccttct tcccatgcat ggatgaactt aaagcaggaa atcagaaggc
     3301 tgataaatac cagtacacag aatacggttc caagaccgat gagcgtatat acgctggccg
     3361 tccttaacag gcctccgatg gagctgtcct cctcaacgcc tgcagacata tcggtaaact
     3421 gagcctggat ctgctgtgtt gccatattca ggttcataac atactttttg gttccctctg
     3481 caaactctac aggaaggatc accgtatagc ttccgtcctc cagaagcacc acagagccat
     3541 cctcaatatc aacactgcgg agagcgccca tctcttcccg cacatcaagc tgggcctgat
     3601 aaagctctgc agaaacggta tctccctgag actgggccag cttgccctgg actaccagct
     3661 gttcatcgga agcgcccaga gtctgagctg cggataaaag aagggactgt cccatggcgt
     3721 cttccacagg cgtaccgtcc atgctgatgg ataagctttc tgtttcctcc tctgttttag
     3781 agcaggcaga cagacttacc aggcaggcag atactaaaac tgctgctgcc atccttttaa
     3841 ataatcgtct catatacatg tcacccctct ttaaaccgtg ccatgcttct ttgaaggacg
     3901 gtcctcactt tttgtataaa gcatatcaaa tgcagctgcc agcctctggc gggtttcctc
     3961 tgccttgatg atatcgtctg catagcctct tcttgcggca gccactgctc cggactgagt
     4021 ctttgtatac tcggcagtct tttcgctgat cagagccact gcatcatcag aagcctcgat
     4081 ttcctttgca tacatgatct taaccgcagc ctcaggctcc atagtgccga tcactgcatt
     4141 tggccatgca tataccaggt cggttcccag gctcttgctt cccatggtaa ggcctgcgct
     4201 tcccagtgcc tttccggtta ctacagtcac ctttggaaca gtagcgtctg cataagcgta
     4261 ggttaagcgg gctgctgctc tggcgatccg tctttcgctg cacatctttg ccttatagcc
     4321 gtttacattt gtgagggtca gcagaggaat actgaatgcg tcgcagaagc tgatcagctc
     4381 cactgccttg tcacagccct ttccggataa agcagcctca aactcatctg ccttctttcc
     4441 atcctcataa cgctctgtgc ggtttgccac acagcccaca gtcacgccat ttaaacggat
     4501 gaatgcagtt accatagaag gttcataatc cttctttact tccattacaa aatgattatc
     4561 agaaatcatg gataatacct ggataggatc tcccgccaga ccttcaatgc cgctgcacat
     4621 acggtttaaa tcatcctggc agtcctcctc ggaaagatcc tcttcgttat tagcaggaag
     4681 aatggatacc agagtacgga tctgagacag aatggatgct tcatcaccgg taaaatcaac
     4741 aacgccggac tccttgctct ggaatgcagc tgccgctgta tcacattttg ccttataatt
     4801 gccctctaat gcattgggag catttacaaa gagctgcgcg gacttctctt ccataaaggt
     4861 aaaatctgcc attccggctg ccacagccat tccgccgccg caggtaccga atactgccat
     4921 gatctgaggg atcacaccag atgccattgc ctggcttaaa tacagctttc caaagccatc
     4981 cagcgcatct gttgcctcct gcagacgcag tccggcacag tctaccagac cgatcacagg
     5041 tgctcccatc ttcatagcca tggaataaat gctgcagatc ttctttgcat gcatctcacc
     5101 catggagcct cccagtacgg atgcatcctg gctgtataca taaaccaggc atcctccgat
     5161 ggtgccgtag cctgtaatca caccgtcagc cggtgtttcc tgctcagcca tgttaaagtc
     5221 cgtgtttcta gcggtgatat acgcgccgac ttcaacaaaa ctgttctcat caagcaggga
     5281 tgcaatacgg ctgcttgctg aagtttgtgc tgaattactc attttccagt cctccatcat
     5341 tctaaagttt acaatactgg ctggaactgc aaaaaatccc cttacagttc cgcataaaaa
     5401 caaaaatact gccatatatg attataataa gatttttgtc aaatgacaag accccagaac
     5461 catgaaattg cctgttttcg ataaattatt aacaaatttt ttcaaaaaaa cgggcgcagt
     5521 ccccatgcct gcatccagag agttcctctc gctttatgca ggtacatgaa acagcgccca
     5581 ttttttatct ttcatactgt atattttgac atttttcagc aaaacacatc tgcattttcc
     5641 ataaaaatac aattacaccg tgaaaaaccg gtcatgaatg gcctgtaccg cctgatctgc
     5701 atccctacgg tctaccagca cagaaatttt gatctcgctg gtggaaatca tattgatatt
     5761 gatccccgca tcatacagcg cctcaaagag ctttgcagct actcccggat tattgatcat
     5821 gcctgctcct acaacagaaa ccttagccac atcgcagttc gtctccatac ggctgtagcg
     5881 cagctcctct ttatgctccg tcaaaagctc tgctgtttta ttcagatcct tttctgccac
     5941 tgtaaagcag atatccttcc cttcctcatg gccaatgccc tggagaataa tatccacatt
     6001 tacatgattt cttgccaaca gagagaacac cttaaatgag gttccgatct catttggcac
     6061 gccaataagg gcaatcctcg caatatcctt atcctttgcc acgctgctga tatatgaacg
     6121 ctccatacgc tttgcaactc ccttcacttt tgttcccgga tggcctgtaa agcttgacag
     6181 gacctccatt tttacattgt actttcttgc cagctccacc gaacggttat ggagcacctg
     6241 cgctcccagc gtcgccagct ccagcatctc attatacgtc acctcatcca gctttctggc
     6301 tcctgcaacc tttctgggat ctgccgtgta aacaccgtcc acatccgtat aaatctggca
     6361 caggtcggcc cccagaaccg cagccagagc taccgcagag gtatccgaac cgccccgtcc
     6421 caaagtggta atattctggt tccggtcgat tccctgaaat cctgtaacaa tcactatttt
     6481 cttctgattc agctccgcct caatgcgctc cgtatccacc tttcggatac gcgcatctcc
     6541 cgaaaccgta ttcgtgctga tgccggactg ccagccggta agagaaatca ccggatatcc
     6601 caaagcctca atcgccatcg cacagagcga taccgaaatc tgctctcctg tagacaaaag
     6661 catatccatc tctctggccg aagcctttgg gttaatctcc tctgccttct gaatcagatc
     6721 atctgtcgta tccccctggg cagacaatac tgccaccacc tgatttcccg ccttatatgt
     6781 ctctgtaatg atccttgcaa cattgcgcag ccgctccgtg tcagccacag aggtcccgcc
     6841 gaatttctgt acgatcaaac tcatatctgt tttccttcct taaccccaaa ccagggtaaa
     6901 tataaagagc catggccaaa cggccaccgc cctaacctat accatatttt actcatatga
     6961 acccttttgt caacttgttt cctctttgag tttccgcact ttacactgtg ttctggtgtc
     7021 tgcaaaatac agaaactcaa gttttcctcc ccggaacacc tccgccggag attgcgcccc
     7081 ctgtgccagc cttcccatgc cggtctgcca gggcgacttt ggaggccatt tagaaagaca
     7141 tagaggctgg aatttttcgt taagctccat atcaaacctg tctgagcacg gcgagttttt
     7201 gatatggagc ttgcttttag aaaaatttca gcctcttaga ctttcttatg accggaaacc
     7261 ggagccatgg aagaccggca tgggaaggat ggtgtagggg gtgcaattcc ctctcccatc
     7321 accccttgac cgctcccatg gtaataccct gagtaaagta attctggaac atcaggaaca
     7381 ccgcaacaat gggaaccgcc gccagagccg cacccgccat gatgactccg tagttggtgg
     7441 agaactcctg ctggagtgtc gccacgccca gagaaatcgt caggttcgtt ctggaattta
     7501 acattactaa ctgcatgaaa tagtcattcc atgtattaat aaaggtaaag atcgcaagcg
     7561 ctcccagacc cggctttacg atcggcataa caatgcttgt aaaggtacgg atttcaccgc
     7621 ttccgtcgat ctttgcagcc tccaaaagct ctgtaggaac attttcactg aactgcttca
     7681 tgaggaagat accaaagggc cagccgcaca aaggaaggat aacggcccat ggagtatcat
     7741 ggatccccat aaaggaaacc agcttcatca gcggcaccag gattacctgc tttggaaggg
     7801 ccattgcgaa tacaaaaata ctgaacagca gcttatcgcc gtaaaagcgc ttctttgcca
     7861 gcacataacc ggccatagag gctgccacac agacgatgat cagcgttaca ccggcgataa
     7921 agatactgtt gatcagccac tggagcgccg ggttctgaaa cagcttctca aagttttcca
     7981 aggtcgggtg aagagggaac cattccggag gcagcttgat cgtaaccttc tgggatttaa
     8041 aggctcccgt caaaatccag tagaagggga aaataaatac cacactgaga aggagcagaa
     8101 gcaccataga ggcggcatcc agaattttca ctttattttt catcttctca tcccccttta
     8161 atattcaacg tcgctgccca atgccttaaa ctggacaaag ctgataaaac caatgatcaa
     8221 tgcaagtatt acgcccatgg cattggcgta gccatattcg aacttcttaa atgctgtctc
     8281 atacagatag tacatgatag tagtcgttct gtaattgggt ccgccggaag taagcagctg
     8341 gatcaaagag aagcactgga aggagttgat ggtagtgatc accacgatat acagtgtggt
     8401 gggcagaagg ctgggccatt tgatcttcca gaatacctga aaatcattgg cgccgtctac
     8461 ctgagctgct tctacataat ccttgggaac atttcccaga gaagcgatat agagaataat
     8521 gggctgtcct acgctggtag tgatcagtat aatcagaatg gcccagagag catatctttt
     8581 atctcccgtc cacataatgt tcttttcaat cagatccagt gattttaaga tccagtttaa
     8641 aataccgttt aaagggtcgt agatccattt ccagacaact gttaccgcta cagtacccgt
     8701 aaccacaggc aggaagaaca cacatcggaa gaaggatctt gtaaatggat tcttctcgta
     8761 agtcatggct gctacaaaaa gcgcaaacag tacagtaatg ggaacggagc ctactaccag
     8821 aagcagggtg ttcaccaggg atttctgcca tacaggatct gcaaacatag tcatgtaatt
     8881 agacagtcct gtaaaggtca ttcccttcat attataacgg aaaaaactgg taactacgcc
     8941 cattcccatg ggaaccagca caaacagcac aaaaaacagc atggccggca gcaggaatgc
     9001 ataggaaacc agcgtttccc tcattctgat cttattgatt ttccttgact tcatggcagt
     9061 tctctccttc taaaaatgac tccccatact acaggctgta atatggggag aatacctgtc
     9121 ttattgtgcg tttacgatag actggtttgc ctttgctaca aagtcgtcca gagcagcctt
     9181 tggctcggaa tcgccgttga tcactgcctg gagcatcggg aaccagtagg ttctcatctc
     9241 tgcaaagccc ggaatggtgt tgtagtatgg agaatagtat tttgtaagag aagcgtagaa
     9301 ggacatatcc tcatttcctt catacagatc gcccatggac tggcgaaccg ggaatacgct
     9361 ggtctttact acgttctttg gtccccactc ttcatcattg cagatgaaat caataaactt
     9421 cttggatgcc tctgctcttg cctcgtcgcc gttgtcaaat acgcagaagc cgtttacaag
     9481 atactctaat gcaggtgttc cgtcctcaga tgggaatgga agtgcgattg cctcgattcc
     9541 gttctccttt aatgtctctg ctctctggct tgcaagtcct ggagaccaga gggttgtaaa
     9601 tgcggtattt cctgctacaa actgatctac gtcctcaccg ccgttatatg cggagccgtt
     9661 taacatatat ccgttgtcta cccactcttt gatctttgtc atggccttta cgccgccttc
     9721 gtcattgagg gtgtatgtag taatctcagg atctgttaca gaactgtcat acaggtttgt
     9781 aacgaatgcg cggataccct ggtcgcctcc ctgtccggag cagtatacgg tgccgcccat
     9841 aaagcctgct tcagaaagct ttttcatcat ttcctcaaac tgctctgtgg tccaggtgcg
     9901 gtcgccttcc aggttgatgc agtccatagc gcctgcttcc tctaatgcat tcttgctcac
     9961 tgccatacag aatgctgcgc tgctgatggg atacatataa tagctgtcgc cggacttgca
    10021 ggagttgatc agagcctcat tgtctacgtc ctttacaaat tcctctgtga acatatcgtc
    10081 caaagctacc agcttgccgt tctgtgcgta ggaaatgata cgtccgggag catcaaagag
    10141 cacatcgcat acagtgcctg cctcgatggc tgccgtgatc ttctccggtc ctgatgtaaa
    10201 atcgatggtc tccagcttca ccttgatatc cgggttcttt gcctcaaatg catcgatgat
    10261 ctcctgctca taggttcctg ccggattgtc accgtcctgt gtaaaggtcg ggaatgccca
    10321 ccatgtgatc tctgtcatgc cttctgccgt ggcagttccc tctgccttat cagaagcagc
    10381 ttcgcttccc tttgcttcct cggtcttagc atcttccttt gtatctgctg tctgcgcact
    10441 tcccccgccg cagcctgtaa gtacggatgc cgccatagct gccgccagtc ccaaagatac
    10501 tattttcttc atacgaaagt tcctccttct cgtttctccc cgttccgtct cttacccccg
    10561 gcggaaacat tttttattgt taaacacatc atagcataat attttctttt tgtcaatttg
    10621 cccgaaaaaa atttcttttc tttttttatt ccgttttttt tcagcaaaat aaataaatta
    10681 aggagcttcg tttttgcatt tttatgcatc taacgaagct cctgtctgat gtactgttct
    10741 attttataca aatactgatg ccgccatcct ttggaaacgt cattttaaat ctcctgcagc
    10801 gcggctatgg cagcatcgat catggcagcg cacttcttta cctgctccat atcctcaggg
    10861 atcaaaccgg gcatgggttt tctcacgctt cccagatcaa gtccctcacg aagctttaaa
    10921 atctccttca tcactgcata aagatttcca tggcaggcgc acatggcata aataatggca
    10981 tccgcatcat actggatctt tctcgccgcc tcatactttc ctttcctgca cagctcaaat
    11041 atttttaaat acagctcagg cattaccccg taagtaccac caatgcctcc gtcggctccc
    11101 atggccaggc cggataccag ctgctcatcc ggtccgttaa atacaacgaa atcctttccg
    11161 ccctccatct taaacatctg gatatcctgt acgggcatgg aactgttttt cacagcagcc
    11221 actcttggat ttttgcacat ttctctgaac agaggcatgg taagagctac gcctgccagc
    11281 tgcggaatgt tgtaaatcac aaaatccgta ttgggagctg cggcagaaat gtcattccaa
    11341 tactgagcaa tggcatgctc cggtaaatgg aaataaatgg gcggaataga cgctaccgca
    11401 tccacgccca ggctctctgc atgagctgcc agctcacagc tgtcctccgt attgttgcag
    11461 gccacatggg cgataacggt catctttccc tttacggttt ccatcacagc ttccagaact
    11521 gccttgcgtt cctccttgct ctggtaaata cattcgccgg aggaaccgcc cacatagacg
    11581 ccctttactc cttttttcaa cagatgctct gccagggctt tggtccgctc tgtgctgatg
    11641 cttccatcct cctcgtagca ggcataaaat gccggaataa tcccctgata ctttgtaata
    11701 tctctcataa tgctccacct ttctttccta ccggatatct ccagtataac actttaatta
    11761 tttttttcaa caaaaatata atggtaaaaa ttttttcttt ggtcattgtg tttaaaccgt
    11821 tccaatggta tgataggcgt aagaaaagga ggaaatgctt tatgaaaaaa catattgtgt
    11881 gctttggaga ctccaatacc cacggatact gcgcagaaac cggcggacgc ttctctgaaa
    11941 aggaacggtg gacttgtctt ttacaggagc ttttaggaac ggaatatctg gttctggagg
    12001 aaggcctgtc cggaagaacc tgctgttttc ccgatcctct ctatgaaggg cttaacggcc
    12061 ttgattccat ctatccctgc ctgatgagcc acgagcctgt agatctgttg gtcatcatgc
    12121 ttggcaccaa tgataccaag gaacgctttg gcgcatcctc tgcatgtatc gcacttggat
    12181 taaaacgtct gatcgaaaag gcccggtcca tttctgactg ttggagaaac ggtcccaata
    12241 ttctgatcgt aacacccaaa aatatcgatc ctgaatacgc cggttccagt gtaggaaata
    12301 caatggggcg gggctgtgca gaaaaatctg ccggactggg ttcagaatat gaaaaaattg
    12361 cccttcttac agggtgccgc tattttgatg ccaacacggt tgtgacggaa aacaatcaca
    12421 tcgatttcat gcatctcact gctcaggggc atcgggaact ggcagaagct ttggcaaaac
    12481 agattccctg tctgattcca taaacattaa aacgcagtaa cgtacctgtc tccacaaata
    12541 ttgtgcgaaa ggcagatagg ttgctgcgtt tttgttcatt tactagcaga gcttttccaa
    12601 taccgaccct gatgtctttt cattgttcac actgcactgt tcaaagtaac ggcggtaata
    12661 ttccatatag atcaggtcca ccagaaacag ctggctgatc tctgccgagg tagacccgcc
    12721 ctgaagaggc ccctcattgg ctccggataa aagggtgaca tccgaatagg cgctcagagg
    12781 tgatttcaca aaacgggtaa tacaaataat cttggcgccg gaccgcttcg ctacctctgc
    12841 cacatggatg gtatccttgg ttgctccgga ataagaaaaa ataaccgctg tatcccgctc
    12901 atccatagta gccgcagcca tggcctgcat atggctgtcc tgtatacaaa ataccttgtt
    12961 ttcaatacgc ataaacttat tcattgcctt catggcggta agcatactgg ctcctacgcc
    13021 aaagaaaaac actcttctgg cccggtggat cagtgtgatg gcccggtcaa tatcctcctc
    13081 ctttatcatg gaaaaagttt cattcagggc attgatattg gtatttaata cctttctggc
    13141 cagttcccca aaggaatcct ccatagaaat attgttccct gtaaactggt ctacctgctc
    13201 ctgtccctta ctgatgctta aggacagcat catcttaaat tcctggtatc cctttaaatc
    13261 catagtcttg cagaatcgga acacactggt gtctcccacc tcacaggcct ccgccagatc
    13321 tgtaatggac ataaacagaa cgtcccttgg atttgccaga atataatctg ccactttctt
    13381 ttctgccttg gtaaactgat tatatgcgga tttgatactc gttataaaat ctccctgcat
    13441 acgatccatt cctctcaatc tgtataattt cttccatttt atccagtttc ttttgccttg
    13501 tccatacttt ttcaggcttt tatctgattt ttcatttatt gtaactttcc gttttctttt
    13561 cttttttcct ctctccccag ccaggcagct cccaaaagac cggctctgtt tcccaactcg
    13621 gcaggaagaa tattcacatc cctgaagctt ggcataatgc gctccatagt acgcactctt
    13681 acctgctctg ttacataagg ctgggccatg acgccgccgc ctaagatcac acaggacgga
    13741 ttaaaaatat ggatcagcgt ctgcagtccg tttacaatct catcgatcca ctcatccaca
    13801 gccgcctgca cccccggctg gtcgatagcc acaaatatct tcctcccgtc tgtaagcgct
    13861 ggatcaatga ccatggctct ggctgcaagt gctgtggctg aagcgtattt ctcatagcag
    13921 ccgctgaaaa actctccctc tttccgatcc tcaggatgaa tcagaagacc gccaaattct
    13981 cctgcggaaa aggcgcagcc atggtacacc ttctgatcca tcacaatcgc tccgcccaca
    14041 ccggtgccgt aggtcagaca gagaaaatcc ctctctcccc ttcctgcgcc gaagattcct
    14101 tcacccagtg cggctgcatt tacatcattt tctacggcta caggcacatg aaaacgctct
    14161 ctcaacatgc ggccgatctc cattcccgta taaccgggaa tgttttcatt ggcatagcgt
    14221 atcattcctc tttgggaatc tacctgtccg gcggtgctga tcccaatggc atcaaaacca
    14281 tgatagcttt ccagaatctt tacagcctgt tccactacat gggcacctcc aagccgggca
    14341 ttggtcggag tctcccttac ttcttttatt tcgtccttta ttcccgtcca tatccctgac
    14401 ttaatggcgg ttcctccgat atccagtgct gctgtcccca tcgtgctcct cctatcggtt
    14461 cttcactgca tccataaaac ggtttgcaat ctctaacggt cttgtgatcg ctccgcctac
    14521 cacaatggca aacgctcccg catccagcat ttttaccgcc tgatctgggt aatggatttt
    14581 cccctctccg ataaccggaa cgtccacagt ctttgataaa cggcttacca gctcataatc
    14641 cgggccgtcc atatgaggag aatctgccgt atagccgctc atggtagttc ccacaatatc
    14701 cattcccagc ttccaggcat tgattccctc ctcatagttt gatatatctg ccataagaat
    14761 ggcatccgga tatttctggc gcacctcttt tataaactca tttacggtct ttccgtctcc
    14821 ccgtgcgcgc agcgtacagt ctaacgcgat cacatcgctt ccaacagcga caagctcatc
    14881 gacctccttc atggtaggag taatgtagct ttcaaagccg ggatactgga tctttaccag
    14941 tccgataacc ggaagtcctg tctcctcctt gatcgcagct acgtcccgga tgctgctggt
    15001 gcggatcgcc ggagtccccg ccatctttgc cgctctcgcc atcaggtaca tcacagattt
    15061 ttcctccaca tacagaggtt ctcccggaat agcctggcat gagatgatca tctgcccctt
    15121 gatctgctca aatagttcct gcttgttcat gtatctcctc ctgttctgca accaccctta
    15181 cagacattcc ccattttgcc caaaaggtcg atgccatgaa atatttcttt tataactata
    15241 tcataaagaa atattttttt caattataaa tgtgttgaaa tattttttta ttactcattt
    15301 taaaaacagg actgccctga gaatggcatc tcacagacaa tcctgtttcc atttcaaacg
    15361 tgttccatca gatattcggc atctttgccg ttggcatcat cagcaccttt cctgtgccgc
    15421 ggtatacatt taccaggcct tcgcccgaag ctgcggagcc gatcaggctc tttccggagc
    15481 gctctaccgt aaagtccagg ctcttgctcc aggcaatagc ataattgccg tccaccttga
    15541 gcacatcgtt ctgaagctca atctcgatca actcctcttt cgggcattca gattcgatac
    15601 agaacactcc gttcccattc aggctcaggt taaacagtcc ctctcctccg gccactgcag
    15661 aagagaaatt ggaacgcatc accgctttgt gctgcagggt agattcacag gctaaaaaca
    15721 gaccgtcatc cagcaccact ccgccgcccc actgggctgc atccatcagg atcagatact
    15781 tataggtagg ttccagcacc agaacgccgc ttcccgtata ctccggcttt atggcggatt
    15841 ctccggttgc cttgccccgg accgctttgc cgaggaaatc tcccacgccc ttaatgccgg
    15901 tggttgcctt tacatctcct aacatccact gcatggcgcc tgcctgaatg gtcacattag
    15961 actgatccag actgcatacc agctggcgct ttctcacatt catctgcgcg cagtaataag
    16021 cggttactgc cgatccggga gtcacgctta agtcgcgctg atattcgatt acctcaaagg
    16081 ctcccagctt tgcaaggact tttacatcat cattgtcggt gaaattagaa atacggtaca
    16141 tagatttcct cctgttctct ctgatatcgc agcctgcggc agatatcgtt tgattttatt
    16201 tttccagatt cttccacttg aagtttggaa tcacatcaga ccacttttca attccgataa
    16261 tagacttggc aaactctgag gtttccagag tcgaattttt cttgtcaata tcccggaata
    16321 cctgccagat cttcttgcgt ttcgttgcta cgatcttatc cggctgattc atatcgcact
    16381 cccacaggcc gaacttcagt ccctgctttg cctcgctggg agcatccacc tggcggctta
    16441 ccgtatatgc gtcaatatat ggattactgt ctacaaggta gtaggagtac gcataggcag
    16501 ctgcctggat ctcaggaata tcgcctctgg tgggactata ggaggtaaag ccctgctcgg
    16561 tcagaataat atgacgcaca tttcccgaag gagccttcag tgcctcctgg ttaaagtaat
    16621 cggtaagtac gttcagattg gcaaaattta taactggtga gttaaaatca tttgtaaaga
    16681 gcccggtagc tttgggatca tcccagaatt ccggctcaat catgggacag ggatacggat
    16741 gataggccag tccccaatcc atctgcccct gaacattagc aatggaattg aagctgtcta
    16801 cgatctcctt gccgccgtat ttcagctttc cgtcaatctc attcatccag ttgtaatcca
    16861 gtgagaaata cacccggtca ttggcattgg tactcttgat ggccgtgtag aaaatacgga
    16921 acgcctgctg gtatactttt acataatttg taagatccat agagcccata tagttccact
    16981 gctgattatt gatctcattt ccaatgatcc agttggaaac ctttccatgg ttggaatctt
    17041 ttccgctgta gtggtcagcc aaaaaggccg caatggctct ggtctgctca aaaccggctt
    17101 cgtttgcccc gttgaacata taataaaagg cattggaaga cttcttcgta cccggataga
    17161 tcagatccga acggttgggg ttccagtcat ttaaaatgat caccgtgacg gtcattccct
    17221 tattggaaag agcgctgatg gttctgtcat aatcatccat gatggcctta ttaaaatgat
    17281 agatcttgcc gtcatattca tattcaatgc ctgtacccat gatctgtgaa aacgcaatat
    17341 tggtggttac atgctttacg cccaggtcaa aggcatcctc cagcatatca atttcgatgt
    17401 tcagtccctt tttagtcaga ggatccttga atgcttccgt atttttggcc actgcctcag
    17461 gattggtaat gtaatgaggc tcgctgatgg ctttatactc tgtgccgtca taagcagcca
    17521 gtacaaattt gctgtacagg cggtctgctg tgcttccgaa atttaacggc acctgagcag
    17581 aggatgatat aagggcgtct gccgaagcaa tataatcagt tcttccatcc agactgttct
    17641 cataggtctg ctgttcaaat atatagacct ttccatcggt gccggctgta tcgccgctgg
    17701 tggaaaaggc gatttccaca ttctgcttgt ctgccgtcac cttacaggaa cggatctggc
    17761 ctgtgagcgc aatatcctct tttgctgcct cctgtgcagc ttcacctgaa gcctgtgttt
    17821 cctccgcgga tacagacctg gaaaaagcac ccaggcttac tgctgtcact ccaaaaaaca
    17881 gcgcggctgc cagagccatg cctctgcttc ttttaaacag tttatgcttc attttgagat
    17941 tacctccaga attgatgtgt gtacttacgg atgacccgtt ttgatgtcaa cctctatgat
    18001 acataatcta tcttacaaat tcaattacga ttttattaca ttatcttaag tcttacttgt
    18061 ataccttctt taaagtaaat ccccgcccct gaacctcatt gatctgattg atgatcataa
    18121 acgcttccgg atcctcatcc agcaccagct ggttcagcct tggcagatcc cgcttggaaa
    18181 tcacagccag caccatatcc tgttcccggt gaaggtaacc ggtttccatg tggagaaggc
    18241 tggttccgca gtccagccgc tggctgatca gggcattaat ctgttcatgg cgtcggctta
    18301 ctatcttcac ctgcatcttt gtcatgccgg ccatgagaac cttatccagc accagggtat
    18361 aagtcacgat cagaagaatg ccgtaaagca ctgcctctgc ggatgaagta atgagctgca
    18421 ttgccaggat cacgcagtcc agtacataaa gagacaggga tacattgatg ccgcacttct
    18481 tgcgcaggat cagacagggg atgtccacac ctccggtaga agcgcctgcc tttatgacga
    18541 tcccgattcc cgctccaatg agaataccgg cataaattac ggctaccagc cgttcttcca
    18601 tgtaaaagcc atgtattccc gtgcgctcca ttactgccag ggcagcggga taaaacacgg
    18661 tgcttaaggc cgtggtaagg gcaaattcct ttcctaactg ccacagcccc agaccaaaga
    18721 ccgcaatatt aaaaatactt acaaaaaggc tcacagggat tccccagagc cggttaaaaa
    18781 acaggcccat gcccgtagta cctccggtta caagcccatt cggcatgata aacagcacta
    18841 cggccatggc ataaatcgca tttccaaata aaatggctcc cacccgtgcg gtcatacgaa
    18901 gccatttttg tctgtcattc ttctgttcca ttacaagatt ctcccttatt ttatttccta
    18961 tcaccgaggg tatcattttt catgggagct gtcaagaaac ttgcacttga caatctcctt
    19021 cctgcgttta taatacattt agaacttttg tatctgtttc ctttttcgga tgctgagccg
    19081 gtgtgagggg gagcgccctg atgctgcgta caaaaggata gcgatgggag tccgtgtttc
    19141 ggattgagag gatgcaatcg agcatcgacc gcagagcgca tattctgcgc cctattacag
    19201 ctgtctggaa gttgccgtcg ctatgcctgt attccgggaa catagacttg acgcacgcag
    19261 gtgcgttttt ttgtaccctg aaatgctccc tgccggcatt tcccgcagca gaaaagagga
    19321 aggaatctat gaacaaaaac aaacacgctg caaaactggt ctttcttgcc atgatgacag
    19381 ccctgggagt ggtcatttcc cccattctcc gggtagaggg aatgtgtccc atggcacatt
    19441 ttatcaatgt cacctgtgcg gtcatgcttg gcccctggta tgcctttgtg tgcgcccttg
    19501 ccattggcat catccgcatg acctgtatgg ggatcccgcc tttagcactc acaggagcta
    19561 tattcggagc ctttttatcc ggtatgctct accgaatgtc ccgtggcagg ctggtatggg
    19621 cctttgcagg ggaggtcatc ggcacaggca tcatcggagc catactctcc tatcctgtca
    19681 tgacctttgt ctggggaaaa accggcctta cctggttctt ttatgtgcct tccttcattg
    19741 caggcacgct cattggagga accattgcat ttctgttttt aaagcatctg cagaaagcca
    19801 gactcttatc catgttccag gaaactctgg gttcccggac gtatgattcc ggagaggacg
    19861 tggtaaatga tgcgctggga atcgcatttt caggctttat cggctatctg gctgtgacgg
    19921 tagctgtcca tcagcttgta cctcagggcg gttctgtgat aaacagtctc cgctatatag
    19981 ttctggtcgg ctttctggca gccgccctga tatactggag catcaaacgg cccaaagcag
    20041 cctgaaacgc gcattttccc atttccatac ttacatcaaa ggaggatctc atcatgaatc
    20101 ctatctccct cttgcataaa gaagcacctt taatccactg catcacccac cccattgtag
    20161 tcaatgactg tgcaaatgca gttctggctc tgggcgcacg tcctatcatg gcggagcatc
    20221 ccaaagaagc agcctcgatt gcccaaatag cccgttccct tactgtcagc ctgggaaata
    20281 tcaccgatgc cagggccgaa tccatttttc tggcaggaaa tgtgcgtccg gctgtcatag
    20341 atctggtagg catcacctgc agtgatttcc gtatgaagct tgccagacgg tttctggaag
    20401 aatgccgtcc cgctgtgatc aagggaaatg tttccgagat acgcgccatt gccggcgcag
    20461 gcttccacaa ccaggggatc gacgtaagcc gggaagacgc tgtcactaag aatgacccaa
    20521 tggcacagtt tcgtctggcc cggctgatga aggagattgc agaccgcaca caggccgtcg
    20581 tagctgccag cggtgaggtg gatattattg tatctcctca ggatgataaa gcctattttc
    20641 ttgaaaacgg ttctccatcc atggcccgca tcacgggaac cggctgtatg cttacctgca
    20701 ttatgggaac ctttatggca gtagtatccc cccttgaggc agcggtctgc ggagctgctg
    20761 tcctgggaat tgccggagaa cgtgccgatg cttcaaaggg acttggaaca tatcacataa
    20821 gcctgttgga tcagctttcc ctgatgacag atgaaacgct gaaatccgaa atccgtctgc
    20881 attctgttga tctttcttct acagcttcat aaaaccggac atcatacatc cgcctgccgc
    20941 tcctgtactc aggatctccg gcaggcgttc ttcatttact ccgccaatgg cataaacagg
    21001 aacactgctt tccatacaaa cctggcttaa gaaatccagt cctctccccg gaagcccttt
    21061 cttacaatct gtctcataga tattgcctgc aaacagataa tctgcgccca gccggacagc
    21121 ctcttttacc tcttccaacg agtgaatgga tgttcccaca cactctgcat ccagcggtct
    21181 cccttccctt ctcaatatac tcatcggaag atgaagcttc ctcatgccca gctgtgcggc
    21241 tgctgcagga gctgtgttca aaatcagcat ccgatcctgt tcccggcaga tctgatccac
    21301 ttcctctgcc agcttcctgt aaatctcagg gcttaagtcc ttttccctga gcactactgc
    21361 tctgggtttg acacttacaa tcttccgaat ctgatccaga tagctctccc agagtcccgt
    21421 tcccggttca aaacagccat cttctgtctg atccgccccc tccggcactc tgcagagcct
    21481 gtgattggtt actgctacca tacgttcata cataagctgc atttcccttt ctgtattata
    21541 aaaagccccc ggaaactccg ggggccaact cgtttgggca caatatccat tcagatatat
    21601 catttatcac gttcgtacgg cctgatggcc tgtaacgatt taattactct acgatagata
    21661 caactctacc ggaacctact gtacgtccac cctcacggat agcgaaacgc agaccctgct
    21721 ccatagctac tggatgaatc agctctacgg acatctctac gttatcacca ggcatacaca
    21781 tctctgtacc tgctggcagc tcgcaaacgc cggtaacgtc agttgttctg aagtagaact
    21841 gtggacggta gttgttgaag aaaggtgtat gacggccacc ctcgtccttg gtcagaacgt
    21901 aaacctgagc ggtaaacttg ttgtggcact ttacagaacc tggcttgcac agacactgtc
    21961 cacgctcgat ctctgttctc tgaacaccac gcagcagagc accgatgtta tcaccagcct
    22021 cagcgctgtc aagaagctta cggaacatct cgataccagt tacaacggtc ttacgaacgt
    22081 cctcatggat accgatgatc tcaacttcgt cgttcaggtt cagagtacca cgctctactc
    22141 taccagtagc aacagtacca cggccagtaa tggtgaatac gtcctctaca ggcataagga
    22201 atggcttctc tgtctcacgt acaggatctg gaatgtagct gtcaacagcg tccatcagct
    22261 ccataacctt gtcgccccat gggcccattg gatcctcaag agccttcaga gcagaaccct
    22321 ggatgatagg tgtatcatca cctgggaact cgtactcatt taacagctca cggatctcca
    22381 tgtcaaccag ctcaagcagc tcagcgtcgt ctaccatatc gcacttgttc atgaatacta
    22441 cgatataagg aacgcctacc tgacgggaca gaaggatgtg ctctcttgtc tgagccataa
    22501 caccatcagt tgcagctaca accaggatag cgccgtccat ctgagcagca ccagtgatca
    22561 tgttcttaac gtagtcagca tgtcctgg
//
LOCUS       DBMDUH010000025        22560 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_1489_length_22560_cov_7.047954, whole genome shotgun sequence.
ACCESSION   DBMDUH010000025 DBMDUH010000000
VERSION     DBMDUH010000025.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 22560)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 22560)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22560
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1489_length_22560_cov_7.047954"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="ACZPCG_03735"
     CDS             <1..612
                     /locus_tag="ACZPCG_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006774393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1284218.1"
                     /translation="QALNDLASEGIIFRRQGRGTFVNVDSLDIKVTFSPCMELTQMIQ
                     KSGYTPSVRLLKIRKIKREEEICSLLQMQPDEQLVVAEKLFLADDQICAYCRDYFSMD
                     LIGGEESFEMFSHYEDSIYRYIYDLSGEKAQWDKVEIDTADPADIPGLKGYVSVKELG
                     SRPYLYLKTLNYSDRDRPLVYANEYFNTGIIQFNLIRQKNIQY"
     gene            770..1681
                     /gene="ispE"
                     /locus_tag="ACZPCG_03740"
     CDS             770..1681
                     /gene="ispE"
                     /locus_tag="ACZPCG_03740"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HIR1284219.1"
                     /translation="MISHLRLKAFGKINLALDVLGRKPDGYHEVRMIMQTVGLHDRID
                     LYRTAEPGIRLETNLFYLPSNEQNLAYRAAALLFDEFGIEEGLSIRLRKFIPVSAGMA
                     GGSTDAASVLYGVNRMFGLGLTTSQLMERGVKLGADIPYCIMRGTALSEGIGEKLTRL
                     PSMPQCQVLIAKPGISVSTKTVYEMLDARTLRPEDHPDIDGMVEAIRKRDLYEVAGRF
                     GNVLELVTAGRYPVIGQIEELMKEYGAINAMMSGSGPTVFGLFSNPSSARKAYEALRY
                     GEGIDLAKQVYLTNFYNMNQGAAGRLR"
     gene            1703..2407
                     /locus_tag="ACZPCG_03745"
     CDS             1703..2407
                     /locus_tag="ACZPCG_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1284220.1"
                     /translation="MDNNFKVNMNEYLPLRDVVFNTLRQAILKGELAPGERLMEIQLA
                     EKLGVSRTPIREAIRKLELEGLVLMIPRKGAEVAKISEKSLRDVLEVRRSLEELAIEL
                     ACERMEAEDLRRLEQCQENFCRAIRSGSPMEMAESDEAFHDVIYQSTGNEKLVQMLNN
                     LREQMYRYRLEYIKDADKRQVLMVEHEHILSALKVRNLAEARMAAREHIDNQEITVSK
                     NIKEQEENVLPVRGRR"
     gene            2411..2614
                     /locus_tag="ACZPCG_03750"
     CDS             2411..2614
                     /locus_tag="ACZPCG_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284221.1"
                     /translation="MEEKNFKERYLAGEIEFEEIDRYISRWNNSDDERTLAKYLGLNE
                     EEEDVWIDESDEALREMLDRQKK"
     gene            complement(2651..3301)
                     /locus_tag="ACZPCG_03755"
     CDS             complement(2651..3301)
                     /locus_tag="ACZPCG_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HIR1284222.1"
                     /translation="MKKHPSYFFPFPIFHILPILLLTPFLLLASARTISQETMAARIA
                     PDILRFHVLAESNSPKDQSLKLGVRDILLSHIQTHAPKNATKPQLAHWILQQKNTLTQ
                     TAESWLTSQGSPAPVSLTLTKDYFPTKSYGEASFPCGVYDAVRITIGNGKGRNWWCVL
                     YPSLCLTDSSAKTVPESSKKELAEHMAPDDYETIFNEPPKVEIRFHLLELLTPKKD"
     gene            3465..4526
                     /locus_tag="ACZPCG_03760"
     CDS             3465..4526
                     /locus_tag="ACZPCG_03760"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008719830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIR1284223.1"
                     /translation="MEKKTAAVIFGGQSSEHVVSCMSAANVIDRINTDKYNLILIGIT
                     QEGKWLKVDSVQAIRDESWRKGTVSAAILPDAEKKYALFMENGTMTEVKLDVVFPVLH
                     GLCGEDGTIQGLLELAKIPYVGCGVLASAVSMDKLYTKIIVDELGIRQAAYVPVMKEQ
                     LEDMNKVVNRIEGRFAYPVFIKPSNAGSSKGVSKADNREELEAGLTEAACHDRKILVE
                     EMIVGREVECAVFGGGSEEVKASGVGEILAAADFYDFDAKYYNAESKTVTDPELPGDA
                     AEKIRRAAAAIFKAVDGYGLSRVDFFVKEDGEVVFNEINTMPGFTAISMYPMLWEARG
                     IGKEQLVDMLLEHGLKRFA"
     gene            4563..4988
                     /locus_tag="ACZPCG_03765"
     CDS             4563..4988
                     /locus_tag="ACZPCG_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwiB family protein"
                     /protein_id="HIR1284224.1"
                     /translation="MTKDVLITIRGVHTLDHEDNDVEMIVRGDYYQKNGKHYILYEEI
                     LEGAEERVKNVIKISPSSMDIIKKGVTNSRMLFEKNKKNLSCYSTPVGNLVIGIQANH
                     FYVEEQENSIKVNVDYSLDINYEHMSDCRICVDVQSCTA"
     gene            complement(5073..5525)
                     /locus_tag="ACZPCG_03770"
     CDS             complement(5073..5525)
                     /locus_tag="ACZPCG_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284225.1"
                     /translation="MVQTILNVLLILLAIAAIVLVILYFLGSKLQKRQAEQQQMLDAA
                     AQTVSILVIDKKKLKLTQSGLPKMVVDQTPKYMRWAKVPVVKAKIGPKVMTLIADAGV
                     FESLPVKTEAKVVLSGIYITQIKSVRGGAIPAPPKKKGFFARFKKDKQ"
     gene            5835..9767
                     /gene="rpoB"
                     /locus_tag="ACZPCG_03775"
     CDS             5835..9767
                     /gene="rpoB"
                     /locus_tag="ACZPCG_03775"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005338542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HIR1284226.1"
                     /translation="MEKSRIRPVPAGKSVRMSYSRQKEVLQMPNLIEIQKDSYQWFLD
                     EGLKEAFDDISPITDFAGHLSLEFVDFTLCKDDVKYTIEECKERDATYAAPLKVKVRL
                     YNKDKDEMNEHEIFMGDLPLMTDTGSFVINGAERVIVSQLVRSPGIYYGIGHDKVGKE
                     LYTCTVIPNRGAWLEYETDSNDVFYVRVDRTRKVPVTVLIRALGFGTNAEILELFGEE
                     PKLLASFGKDTSDNYQDGLLELYKKIRPGEPLSVDSAESLLNSMFFDPRRYDLAKVGR
                     YKFNKKLSFKFRLNGQVLAEDVVNEETGEILAEAGTKLDKESAMRLQNSGVPFVWVEG
                     SNRKQKVLSNMMVELDAHVSFDPEEVGVTELVYYPVLSAILEKAGSDEELLKEEIRKN
                     IHDLIPKHITKEDIIASINYNMHLEEEVGTADDIDHLGNRRIRAVGELLQNQYRIGLS
                     RMERVVRERMTTQDVDGITPQSLINIKPVTAAVKEFFGSSQLSQFMDQNNPLAELTHK
                     RRLSALGPGGLSRDRAGFEVRDVHYTHYGRMCPIETPEGPNIGLINSLASYARVNQYG
                     FIEAPYRVVDKTADPANPRVTDDVVYLTADEEDNFIVAQANEALDEEGHFVHNNVSGR
                     FQTETSEFQKRTIDLMDVSPKMVFSVATAMIPFLQNDDANRALMGSNMQRQAVPLLST
                     EAPVVGTGIEAKAAVDSGVCVVAKNAGVVERSASNEIIIKRDSDGNRDVYHLTKFKRS
                     NQSNCYNQKPIVYKNDHVEAGEVIADGPSTSNGEIALGKNPLIGFMTWEGYNYEDAVL
                     LSERLVQDDVYTSIHIEEYEAEARDTKLGPEEITRDVPGVGEDALKDLDERGIIRIGA
                     EVRAGDILVGKVTPKGETELTAEERLLRAIFGEKAREVRDTSLKVPHGAYGIIVDAKV
                     FTRENGDELSPGVNQTVRIYIAQKRKISVGDKMAGRHGNKGVVSRVLPVEDMPFLPNG
                     RPLDIVLNPLGVPSRMNIGQVLEIHLSLAARALGFNVSTPIFDGANENDIQDTLELAN
                     HYVNLPFDEAEKAEWEKDGVTETPSGQPWNGETFEQLYKEQLVPEAIGYLSENRAHRE
                     LWKGVPIGRDGKVRLRDGRTGEYFDGAVTIGHMHYLKLHHLVDDKIHARSTGPYSLVT
                     QQPLGGKAQFGGQRFGEMEVWALEAYGASYTLQEIMTVKSDDVVGRVKTYEAIIKGEN
                     IPEPGIPESFKVLLKEMQSLGLDVQVQREDGTEVEMTENIDYGDTELRAMLEGDRRFN
                     DQESLASYGYQEQEIKDNEFVSVESASESAGNDTDDLYDAYTDDGEDE"
     gene            9787..13638
                     /gene="rpoC"
                     /locus_tag="ACZPCG_03780"
     CDS             9787..13638
                     /gene="rpoC"
                     /locus_tag="ACZPCG_03780"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HIR1284227.1"
                     /translation="MPETNETYHPMTFDAIKIGLASPEKIRSWTHRTPEPADKPSKQW
                     REWWEQGAMRNRMPEPSGAPSREWREWWEHGVVKKPETINYRTLKPEKDGLFCERIFG
                     PSKDWECHCGKYKKIRYKGVICDRCGVEVTKASVRRERMGRIELAAPVSHIWYFKGIP
                     SRMGLILDISPRTLEKVLYFASYVVLDPGVTSLQYKQVLSEKEYREEVEKYGGSTAFR
                     VGMGAEAIQELLMSIDLEKDSADLRKQLVDATGQKRARIIKRLEVVEAFLNSGNRPEW
                     MIMDVIPVIPPDIRPMVQLDGGRFATSDLNDLYRRIINRNNRLARLLELGAPDIIVRN
                     EKRMLQEAVDALIDNGRRGRPVTGPGNRALKSLSDMLKGKQGRFRQNLLGKRVDYSGR
                     SVIVVGPELKIYQCGLPKEMAIELFKPFVMKELVSNGTAHNIKNAKKMVEKLQTEVWD
                     VLEDVIKEHPVMLNRAPTLHRLGIQAFEPILVEGKAIKLHPLVCTAFNADFDGDQMAV
                     HLPLSVEAQAECRFLLLSPNNLLKPSDGGPVAVPSQDMVLGIYYLTQERPGAKGEGMI
                     FKSVNEAILAYENHEATLHSRVKVRVSKTLPDGTVKTGIIESTIGRLIFNEIIPQDLG
                     FVDRSIPENELKLEVDFLVAKKQLKQILEKVINVHGATQTAVTLDDIKAIGYKFSTRA
                     AMTVSVSDMTVPASKPELIANAQATVDHIAKNYRRGLITEEERYKEVIETWKTTDDQL
                     THDLLTGLDKYNNIFMMADSGARGSDKQIKQLAGMRGLMADTTGHTIELPIKSNFREG
                     LDVLEYFISAHGARKGLSDTALRTADSGYLTRRLVDVSQDLIIREVDCCEGKDIPFME
                     IKAFTDGNEVIEDLQERITGRYIAEDIVDPDTGELVMKANHMCTPKRAAAVMKVLDKQ
                     GRKSVKIRTVLSCKSHIGVCAKCYGANMATGQPVQVGEAVGIIAAQSIGEPGTQLTMR
                     TFHTGGVAGGDITQGLPRVEELFEARKPKGLAIITEFGGVVTIKDTKKKREITVTDNE
                     TGNSKTYLIPYGSRIKVMDGQVLEAGDELTEGSVNPHDILKIKGVRAVQDYMIQEVQR
                     VYRLQGVEINDKHVEMIVHQMLKKIRIEESGDSDVLPGVSMDVLDFNEMNEALIAEGK
                     RPAEGKQVMLGITKASLATDSFLSAASFQETTKVLTEAAINGKVDHLIGLKENVIIGK
                     PIPAGTGMKRYRTVKLDTDDKIAAAAEEEILLSEEEELTSEESSELTEDNVAEEVLDL
                     DDTVIEDEDAEEEESSEDAEE"
     gene            complement(13712..14617)
                     /locus_tag="ACZPCG_03785"
     CDS             complement(13712..14617)
                     /locus_tag="ACZPCG_03785"
                     /inference="COORDINATES: protein motif:HMM:NF024863.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HIR1284228.1"
                     /translation="MKKYWYTMMLIGSLIAVKGSSLIPRIWELETFSGEHKIERLAET
                     EDKQPSNIPLSSDSISESAASKGSTETPTAKFPSPESSPAEPPTDGSAPVTESSTEAP
                     PAETLPPADTSVPVDLSTSLFIGDSRTEGLKEYGGLEEAEFFANSGMSSFNLLSTNLT
                     VKDGSKKTLEELLSQHSYERIYLMLGINELGYPPSSVTKKYASIVDWIHQLQPQARII
                     LEANLHVTKKKSDSDPVYGNGKIDSLNQAIYQMAVEKGCGYIDVNSLFDDGQGNLDSK
                     YSVDNAHIMGKYYTVWAEWLRTQNA"
     gene            complement(14663..16081)
                     /locus_tag="ACZPCG_03790"
     CDS             complement(14663..16081)
                     /locus_tag="ACZPCG_03790"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HIR1284229.1"
                     /translation="MIFSSFDFLFRFLPLFLILYFICPEKWRNQCLFAGSLIFYFYGA
                     RHSPLYFVLFLLSIPVNFFIGQLIGRKRDAKERSFWLNAGIFYNLFWLILFKYSTFIT
                     ENLNLLLSYTKTGLQFPVIRLSLPLGISFYTFQAIAYLADVYKKEIRSEHSLLRFCTW
                     FSMFTQVTSGPITRYQEIRSQLIRRRFSMDRLETGLREFTIGLSLKVLLANQLGRLWS
                     QVGAIGYESISTPLAWMGIIAFSLQLYFDFYGYSLMAQGIGWILGFQIPRNFAYPYQS
                     LTMTEFWRRWHITLGKWFRDYVYIPLGGNRKGFVIMVRNLLTVWLLTGFWHGADWNFL
                     LWGLLLFALISIEKMGLGRILNRFPAAGHLYMALVIPLNWLVFAISQPPQILIYLQRL
                     FPFFTAPGTYTWFEGDYLKYGRIYGLSLLAGLIFMTDLPRRIYSRHRRSFLTAVILIV
                     LFWLCVYCIKMGMDDPFLYFRF"
     gene            complement(16300..16770)
                     /locus_tag="ACZPCG_03795"
     CDS             complement(16300..16770)
                     /locus_tag="ACZPCG_03795"
                     /inference="COORDINATES: protein motif:HMM:NF015378.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HIR1284230.1"
                     /translation="MRKMNLKKAMMAAVVAAMALVTVIGSTITASASPRTAKKIIGIE
                     QAKEAALKAMKIKEADAVFTEQECELDDGVYELEFTAGKFEYDVEVNAYTGKVIKADY
                     ENNRDWDNLRDDCWDDDRDNSWDDDDRWDDDDRWDDDRFDDDDRFDDDDDHWDD"
     gene            complement(16920..17831)
                     /locus_tag="ACZPCG_03800"
     CDS             complement(16920..17831)
                     /locus_tag="ACZPCG_03800"
                     /inference="COORDINATES: protein motif:HMM:NF048641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-I factor RsgI family protein"
                     /protein_id="HIR1284231.1"
                     /translation="MKYLIMECHMAYAVVLDEDGRFLKAANMDYEVGQTVDTIMEIVE
                     PGAAFSEADCQSDSFYDTVFEEAVEVQPAVRRTSRLSRWLKSALPAAAALCLLLSGGH
                     YFIMSPYGTVRLSINPEVELSVNRLDYVLSLSGINRDGEDLIRGYDYKRKKVEEAAAG
                     LAERAIDMDYLAPGGTIYVGVSSAHEDWADEMKRDLTWELDGRLGSDIHISADPKPDE
                     SPESGTAREAETAFPASPPVSETVPAAVQTAPAAHQTVPTAHPNDNWNEDSDEQRDED
                     WDDTTNHGSDDNQEDDWDDAADDDSND"
     gene            complement(17828..18556)
                     /locus_tag="ACZPCG_03805"
     CDS             complement(17828..18556)
                     /locus_tag="ACZPCG_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007037097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HIR1284232.1"
                     /translation="MKFEEHKIVQQVMRSRQDPEAADQLIRSYMPFIKAETARFIHRP
                     PVEGRDDELSVAMFAFYETMTVYNPDRGPFLPLAAAGIRNRLIDYFRKEQKHSGQLSL
                     DQPAEEDGCTLLEKTDPGVDDISDYQDLAAAQSEIETFSAELAGFGLSLTDVADSCPR
                     QERTLDACMKVLDYAKKHPELLEQMIRSRKLPLSQLALGSGAEKKTLERHRKYLIAIL
                     LAFTNGFEIIRGHLSQMKKGGAAE"
     gene            18828..19247
                     /gene="rpsL"
                     /locus_tag="ACZPCG_03810"
     CDS             18828..19247
                     /gene="rpsL"
                     /locus_tag="ACZPCG_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004842433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HIR1284233.1"
                     /translation="MPTFNQLVRKGRQSSAKKSTAPALQKGYNSLQKKATDVSAPQKR
                     GVCTAVKTATPKKPNSALRKIARVRLSNGIEVTSYIPGEGHNLQEHSVVLIRGGRVKD
                     LPGTRYHIVRGTLDTAGVANRKQARSKYGAKRPKDKK"
     gene            19480..19950
                     /gene="rpsG"
                     /locus_tag="ACZPCG_03815"
     CDS             19480..19950
                     /gene="rpsG"
                     /locus_tag="ACZPCG_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HIR1284234.1"
                     /translation="MPRKGHTQKRDVLADPIYNNKVVTKLINNIMLDGKKGVAQKIVY
                     GAFNRVAESTGKDAIEVFEEAMNNIMPVLEVKAKRIGGATYQVPIEVKPERRQALALR
                     WITLYSRKRGEKTQEERLANEIMDAANNTGASVKKKEDMHKMAEANKAFAHYRF"
     gene            19993..22110
                     /gene="fusA"
                     /locus_tag="ACZPCG_03820"
     CDS             19993..22110
                     /gene="fusA"
                     /locus_tag="ACZPCG_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIR1284235.1"
                     /translation="MAGREYPLERTRNIGIMAHIDAGKTTTTERILYYTGVNYKIGDT
                     HEGTATMDWMAQEQERGITITSAATTCHWTLQENCKPKPGALEHRINIIDTPGHVDFT
                     VEVERSLRVLDGAVGVFCAKGGVEPQSENVWRQADTYNVPRMAFINKMDILGANFYGA
                     VEQIKTRLGKNAICIQLPIGKEEDFKGIVDLFEMKAYIYNDDKGDDISIVDIPEDMQD
                     DAELYRTELVEKICELDDDLMMMYLEGEEPSNDELKKVLRKATCECTAVPVCCGTAYR
                     NKGVQKLLDAIIEFMPSPLDIPAIKGVDLDGNEIVRHSSDEEPFAALAFKIMTDPFVG
                     KLAFFRVYSGTLNSGSYVLNATKGKKERVGRILQMHANKRNELDKVYSGDIAAAVGFK
                     TTGTGDTICDEKNPVILESMEFPEPVIDIAIEPKTKASQDKMGDALVKLAEEDPTFKV
                     RTDEETGQTIISGMGELHLDIIVDRLLREFNVEANVGAPQVAYKETFTKAVDVDSKYA
                     KQSGGRGQYGHCKVHFTPMEANAEETFKFTSSVVGGAIPKEYIPAVGAGIEEAAKTGV
                     LGGFPVLGVWADVYDGSYHEVDSSEMAFHIAGSMAFKDAMQKGNPILLEPIMKVEVTM
                     PEEYMGDVIGDINSRRGRIEGMEDIGGGKMVKAYVPLSEMFGYSTDLRSRTQGRGNYS
                     MFFEKYEPVPKNVQEKVLADHKK"
     gene            22211..>22560
                     /locus_tag="ACZPCG_03825"
     CDS             22211..>22560
                     /locus_tag="ACZPCG_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR1284236.1"
                     /translation="MAKAKFDRTKPHCNIGTIGHVDHGKTTLTAAITKVLSERVAGNA
                     KVDFENIDKAPEERERGITISTSHVEYQTEKRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVVAATDGVMAQT"
BASE COUNT         6172 a         4868 c         6077 g         5443 t
ORIGIN      
        1 caggccttaa atgacctggc ctcagaggga atcattttca ggcgtcaggg gcggggaacc
       61 tttgtaaatg tggattctct ggatatcaag gttaccttca gcccctgtat ggagcttacg
      121 cagatgatcc aaaaaagcgg atatacgcct tcagtccgtc ttttgaagat ccggaaaata
      181 aaaagagagg aagaaatctg ctctctttta cagatgcagc cagatgaaca gctcgttgtt
      241 gctgaaaagc tgtttctggc agatgatcag atctgcgcct actgccgtga ttattttagc
      301 atggatctga tcgggggaga ggaatccttt gagatgtttt cacattatga ggattccatt
      361 tacagataca tttatgacct ttccggggaa aaggcccagt gggacaaggt ggagatcgat
      421 acggcagacc cggcggatat tccgggcctg aaaggatacg tttccgtcaa ggagctgggt
      481 tccagacctt atctgtatct taaaacatta aattacagtg atcgggacag gccgcttgta
      541 tatgcaaatg agtattttaa tacagggatt atacagttta atctgatacg gcagaagaat
      601 atacagtatt aaaaatggga gacgtctgga aaatgggcgt ctcttttttc ataagtatta
      661 ctttttgttt attttttata ttgaaaatct ttgcattttc atgtatacaa tgttatgtat
      721 tataaaaaca gacatataaa cgagaaagag aaaaagggga gcggggagaa tgatttcgca
      781 tttacgctta aaagcattcg gaaagatcaa tctggctctg gatgtgctgg gcagaaagcc
      841 ggacggatac catgaggtgc gcatgatcat gcagacagtg ggtctccatg acaggattga
      901 cctgtaccgg acagcggagc cgggaatccg tttggaaact aatctttttt atctgccgtc
      961 caatgagcag aatctggctt accgggccgc ggctctgctg tttgatgaat ttggtattga
     1021 ggaaggactt tcgatccgct taagaaagtt tatccctgta tcagcgggga tggcaggagg
     1081 aagtactgat gctgcatccg tgctttacgg cgtaaaccgg atgtttggac tggggcttac
     1141 aacctcacag ctgatggagc ggggggtgaa gctgggagca gatataccct actgcatcat
     1201 gaggggaacg gccctttcgg aaggaatcgg tgaaaaactt acccgtctgc catccatgcc
     1261 tcagtgccag gtgctgatcg caaagccggg gatcagcgtt tccacaaaaa ccgtatatga
     1321 aatgctggat gccaggacgc tgaggcctga ggatcatcct gatattgacg gaatggtgga
     1381 ggcaatccga aaaagggatc tctatgaggt ggcaggaagg tttggaaatg ttcttgaact
     1441 ggtaacggcg ggccgctatc cggtgatcgg acagattgag gaactgatga aggaatatgg
     1501 ggccatcaat gctatgatga gcggaagcgg gcctactgta ttcggtctgt tttccaatcc
     1561 gtcttcagcc agaaaggcat acgaggcgct tcggtatggg gaagggatag atctggcaaa
     1621 gcaggtttac cttaccaatt tttataacat gaatcaaggc gcagccggca gactgcggta
     1681 aaacacaggg gagagtatta tgatggacaa taattttaag gtaaatatga acgagtatct
     1741 tccgcttcgg gacgtggtgt ttaatacgct gcgccaggcg atcttaaagg gggaactggc
     1801 tccgggagag aggcttatgg agatccagct ggctgagaag ctgggggtga gccgtactcc
     1861 gatccgtgaa gccatccgga agctggagct tgagggacta gtgcttatga tcccgcgaaa
     1921 gggagcggaa gtggctaaaa tctcggaaaa aagtctgagg gatgtactgg aggtgcgccg
     1981 ttctctggag gagctggcga ttgaactggc ctgcgagcgt atggaagcgg aggatctgcg
     2041 caggctggag cagtgccagg agaatttctg cagggcgatc cgcagcggaa gtcctatgga
     2101 gatggcagag agtgatgaag catttcatga tgtgatctac cagagtacgg gaaatgaaaa
     2161 gctggtgcag atgttaaata acctgagaga gcagatgtac cgctaccgtc tggaatatat
     2221 aaaggatgca gataagcgcc aggtgctgat ggtggagcat gagcatattt taagcgcctt
     2281 aaaggtgaga aatctggctg aggccaggat ggctgccaga gagcatattg ataatcagga
     2341 gattactgtt tcgaaaaata ttaaggagca ggaggaaaat gttcttcctg tccgcgggag
     2401 aaggtaaaat atggaagaga agaattttaa ggagcgttat ctggcagggg aaatcgagtt
     2461 tgaagaaatc gaccggtaca tcagccgctg gaacaacagt gatgacgagc ggacgctggc
     2521 aaagtatctg ggcctgaatg aagaggaaga ggacgtctgg atcgatgaga gcgacgaggc
     2581 gctcagggag atgctggatc gtcagaaaaa gtaggatttt gtatgcaggg agggactgac
     2641 cgggagatgg tcagtccttt tttggggtga gaagttccag gagatggaaa cggatctcta
     2701 ctttgggagg ctcattgaaa atggtttcgt aatcatcagg ggccatatgt tctgccagtt
     2761 cttttttcga ggattcggga acagtttttg cggaagaatc ggtaaggcag agactgggat
     2821 agaggacgca ccaccagttg cgtccttttc catttcctat ggtaatccgg acggcatcat
     2881 agacgccgca ggggaaggag gcttcgccgt aggattttgt ggggaaatag tctttggtca
     2941 gagtaaggga tacaggggct ggggagccct gggatgtaag ccagctttcg gcggtctggg
     3001 tgagggtgtt tttttgctgg aggatccagt gggccagttg gggcttggtg gcgtttttgg
     3061 gggcgtgtgt ctggatgtgg cttaaaagga tatcgcggac gccaagctta agggattggt
     3121 cttttggaga gttgctttcg gccagaacat ggaaacggag gatgtccggg gcgattctgg
     3181 cggccatggt ttcctgggag atggtgcggg cggaggccag gagcaggaag ggggttagaa
     3241 ggaggatggg gaggatgtgg aagattggga atgggaaaaa ataggaaggg tgttttttca
     3301 tagggcgttg ggggctcctt tcttggattt ttgggtcgcg atcgccgatt tgggtcgcga
     3361 aagccgccgt cctgattgtt ttggggtatt tctatttatc tccacatatg ctatactgta
     3421 tacgccctgt ggggcagaaa gatggaatag gagtgaaaat gacgatggaa aagaaaacag
     3481 cagcggttat tttcggcggg cagtcttcgg agcacgttgt gtcctgtatg tcggctgcca
     3541 atgtgattga ccgtatcaat acagataaat ataacctgat tctgatcgga attacacagg
     3601 agggaaagtg gttgaaggtg gactctgtcc aggcaatcag ggatgagtcc tggagaaagg
     3661 gaacggtttc tgctgcaatc cttccggatg cagagaagaa gtacgccctg tttatggaga
     3721 atgggacaat gacagaggtg aagctggatg tggtattccc ggttcttcat ggcctttgcg
     3781 gagaggacgg cacgatccag ggacttttgg agctggcaaa gataccgtat gtgggctgcg
     3841 gagtactggc atccgctgtt tccatggata agctttatac aaagatcatt gtagatgagc
     3901 tggggatcag gcaggcggcc tatgtgccgg tgatgaagga gcagcttgag gatatgaaca
     3961 aggtggtaaa ccgcattgaa ggccgcttcg cctatcctgt atttatcaaa ccctccaacg
     4021 caggctcttc caagggagtg agcaaggcgg ataaccggga ggagctggag gccggactta
     4081 cagaggcagc ctgccatgac cgcaagatcc tggtagaaga gatgatcgtg ggtcgtgaag
     4141 tggagtgtgc tgtattcgga ggaggaagcg aggaagtgaa ggcttccggc gtgggtgaaa
     4201 tcctggctgc ggcagatttt tatgattttg acgcaaagta ttacaacgca gaatcaaaga
     4261 cagtgacaga ccctgagctt ccgggagacg ctgcggagaa gatccgcagg gcggcggcgg
     4321 ccatttttaa ggcggtagac ggctacggct tgtccagagt ggactttttc gtaaaagaag
     4381 atggagaagt ggtatttaat gaaatcaaca ccatgcccgg attcactgct atcagtatgt
     4441 accccatgct gtgggaggcc agaggaattg gcaaggagca gctggtagac atgcttctgg
     4501 agcacggcct gaaacggttt gcttaaacga tacagccggt ataaaagaag gaggagaacg
     4561 gcatgacaaa agatgtactg attaccatcc gtggtgttca tactctggat catgaggata
     4621 acgatgtgga aatgattgtc cgcggggatt attatcagaa aaacggaaag cattatatcc
     4681 tttatgaaga gatactggaa ggagcggagg agcgggtgaa aaatgtcatt aaaatttccc
     4741 cttcttccat ggatattata aaaaagggtg ttacgaacag ccgtatgctg tttgaaaaga
     4801 ataaaaagaa cctttcctgc tacagcactc ctgtgggcaa tctggtgatc ggcattcagg
     4861 ccaatcactt ttatgtggaa gagcaggaga acagcataaa ggtcaatgta gattattccc
     4921 tggacattaa ctatgagcat atgtcggatt gccgtatctg cgtggatgtg cagtcatgta
     4981 cagcatagtt ctgtctggat aaaacagaaa cataaaaaag aaatataaaa gcagccggcg
     5041 tctgaaacct tcagataccg gctgcttttg aattactgct tgtccttttt aaatcttgca
     5101 aaaaatccct ttttctttgg aggagcaggg atggcacctc cccgaacgct tttgatctgt
     5161 gtaatgtaga taccgcttaa gactactttt gcctctgtct ttacaggaag agactcaaat
     5221 actcctgcgt cggcgatcag agtcatcacc ttgggaccga tctttgcttt tacaacggga
     5281 actttggccc agcgcatata cttgggcgtc tggtccacta ccatcttggg aagcccggac
     5341 tgtgtgagct tgagcttctt tttatcaatg accaggatgg atacggtctg tgcagcagca
     5401 tccagcatct gctgctgctc tgcctgacgc ttctgcagct tgcttcccag aaaatagagg
     5461 atcacaagta cgattgcagc gatcgccaga agtatcagca atacatttag aatggtttgt
     5521 accatatcgg tatacctcca gactgtaaat aatttccttc tcctttgcag taaaaacttg
     5581 tcatacctgc cagaagacat tctataacag tatatcattt ttgtaagaaa aaagcaagaa
     5641 aaaattcttg acaccttgac gtccctatgt tattatggag tgtgcactat tgtggcgacg
     5701 tgggcttttt gcgcccattc tgcggcgccc gattaccgag aacgacagta atggcccgga
     5761 gtgctggaag agatgcgcat tctccagccg gacgggatat tgcaaccaaa ttagaaaaca
     5821 ggggtgaaac gtcaatggag aaaagcagaa tacgtccggt accggccggg aaaagcgtaa
     5881 gaatgagcta ttcaaggcag aaggaagtcc ttcagatgcc aaacctgatc gagattcaga
     5941 aagactccta tcagtggttc ttagacgaag gactgaagga agcatttgat gacatctctc
     6001 cgatcactga cttcgcagga catctgagcc tggaattcgt tgactttaca ttatgtaagg
     6061 atgatgtcaa gtacaccatc gaagagtgta aggaacgcga tgccacttac gcggcaccac
     6121 ttaaggtaaa ggttagactg tacaataaag ataaagatga gatgaacgaa catgagatat
     6181 tcatgggaga tctgcctctg atgacagaca cgggctcctt cgttatcaac ggtgcagaac
     6241 gtgttatcgt cagccagctt gtgcgttccc ctggtatcta ctatggcatt ggtcacgata
     6301 aggtgggtaa ggagttatat acatgtactg tgatccccaa ccgcggcgca tggctggagt
     6361 acgagacaga ctccaacgat gtattttatg tccgcgtgga cagaacccgt aaggttcctg
     6421 ttaccgtgct gatccgtgct ctgggcttcg gcaccaatgc agagatttta gaactgttcg
     6481 gcgaagagcc aaagcttctt gccagcttcg gaaaggatac ctccgataac tatcaggacg
     6541 gtctgttaga gctgtacaag aagatccgtc ccggtgagcc gttatccgtt gacagcgcgg
     6601 agagcctgct caacagtatg ttctttgatc ccagaagata tgatctggca aaggtgggac
     6661 gttataaatt taataagaag ctgtccttta agttccgcct caatggccag gttctggctg
     6721 aggatgtggt gaatgaggag acaggtgaga ttttagccga ggcaggcaca aagcttgata
     6781 aggagtctgc gatgcgtctg cagaactccg gcgttccctt cgtatgggta gaaggatcaa
     6841 acagaaagca gaaggttctt tccaatatga tggttgagct tgacgctcat gtttccttcg
     6901 atccggagga ggttggcgtt acagaattag tatactaccc tgtgctgtct gctattctgg
     6961 aaaaagcagg ttcggatgag gagcttttaa aggaagaaat tcgaaagaat atccacgatc
     7021 tgattccaaa gcatatcaca aaggaagata tcattgcttc catcaactac aatatgcatc
     7081 tggaagagga agtgggaacg gctgatgata tcgaccatct tggcaaccgc cgtatccgtg
     7141 ccgtaggcga gctgctccag aaccagtacc gcattggtct gtcacgtatg gagcgtgtgg
     7201 taagagagcg tatgactact caggacgtag acggcatcac accgcagtcc ctgatcaata
     7261 tcaagcctgt aacggctgct gtaaaggagt tctttggaag ctcccagctg tcacagttta
     7321 tggatcagaa caaccctctg gctgaactga cccataagag acgtctttcc gccctgggtc
     7381 ctggcggtct gtcaagagac cgtgccggat tcgaggtgcg agatgttcac tatacacact
     7441 atggccgtat gtgccccatc gagactcctg agggccccaa catcggtctg atcaactctc
     7501 tggcaagcta tgcgagagta aaccagtacg gctttatcga agcgccttac cgcgtagtag
     7561 ataagaccgc tgatcctgcc aatccaagag tcaccgatga tgtagtatac ctgactgcag
     7621 atgaagagga caactttatc gtggcacagg cgaacgaggc tctggatgaa gagggacatt
     7681 tcgttcacaa taacgtatcc ggacgtttcc agacagaaac ctccgagttc cagaagcgaa
     7741 ccattgacct gatggacgta tccccgaaga tggtattctc cgtggctact gctatgatcc
     7801 cattccttca gaacgatgac gcaaaccgtg ctctgatggg atccaacatg cagcgtcagg
     7861 ccgtgccgct gttgagtaca gaggctcctg tagtaggtac cggtattgag gcaaaggctg
     7921 ccgttgactc cggtgtctgc gtagtggcaa agaatgccgg tgtggtagaa cgctccgctt
     7981 ccaacgagat cattatcaag agagattccg atggaaacag agatgtatac catttgacaa
     8041 agttcaagag aagtaaccag agcaactgct acaaccagaa gccgatcgta tataagaatg
     8101 atcatgtaga ggccggcgag gtgatcgcag atggtccttc tacttccaac ggcgagattg
     8161 cccttggaaa gaacccgctg atcggtttta tgacctggga aggctacaac tacgaggatg
     8221 cggttctgct ttccgagcgt ctggttcagg atgacgtata tacctctatt catatcgaag
     8281 agtatgaggc ggaggcccgt gacaccaagc tgggaccgga agaaatcacc agagatgttc
     8341 ccggtgtagg cgaggatgca ttaaaggatc tggatgaacg tggtattatc cgtatcggtg
     8401 ctgaggtccg tgccggagat attctggtag gaaaggttac tccaaaggga gaaactgaac
     8461 tgacagcaga agaacgtctc ctgcgtgcga tcttcggtga gaaggcaaga gaagtccgtg
     8521 atacctcctt aaaggtacct cacggagcat atggaattat tgtagatgca aaggtattta
     8581 caagagaaaa cggcgatgag ctttcaccgg gagtaaatca gacggtacgt atctatatcg
     8641 ctcagaaacg taagatttct gtaggtgata agatggctgg acgtcatggt aataagggtg
     8701 ttgtttcccg cgtgcttcct gtagaggata tgcctttcct gccaaatggc cgtcctcttg
     8761 atattgtact gaatcctctg ggcgtgcctt cccgtatgaa catcggacag gtgcttgaga
     8821 tccacttaag ccttgccgca agagcacttg gctttaacgt ttccacaccg atcttcgacg
     8881 gcgcaaacga gaatgatata caggataccc tggagctggc aaaccactat gtaaacctgc
     8941 cctttgacga ggcagagaaa gcagaatggg aaaaggacgg tgtcacagag acgccttccg
     9001 gacagccatg gaatggagaa acctttgagc agctttataa agaacagctt gttccggagg
     9061 caatcgggta tctgtctgag aacagagcgc acagagagct gtggaagggc gtacccattg
     9121 gcagagacgg aaaggttcgc ttaagagatg gacgtaccgg agagtatttt gatggagccg
     9181 ttaccattgg acacatgcat tacctgaagc tgcatcacct ggtagacgat aagatccatg
     9241 cacgttctac tggcccatac tccctggtta cacaacagcc tctgggcggt aaagctcagt
     9301 tcggcggaca gcgtttcgga gagatggagg tttgggctct ggaggcatat ggtgcatcct
     9361 acaccctgca ggaaatcatg actgtaaagt ccgatgatgt agtaggccgt gtgaagacct
     9421 acgaagctat catcaagggc gagaatattc ctgagccggg tatccccgaa tcctttaagg
     9481 tacttcttaa ggaaatgcag tctctgggcc tggatgtaca ggtacagaga gaggatggaa
     9541 ccgaagttga gatgaccgaa aatatcgact acggtgatac cgaactgcgc gccatgttag
     9601 agggcgaccg ccgctttaac gatcaggaat cccttgcttc ctacggttat caggagcagg
     9661 agatcaagga taatgaattt gtaagcgtag aatcggcttc cgagtctgct ggaaatgaca
     9721 cagatgatct gtatgacgct tatacagatg atggtgaaga cgaataatag aagggagtaa
     9781 gagctcatgc cggaaacaaa tgaaacttat catccaatga catttgatgc cattaagatt
     9841 ggtctggctt cccctgaaaa gattagatcc tggacccaca ggacacctga acctgctgac
     9901 aagcccagca agcagtggag agaatggtgg gagcagggtg ccatgcgcaa ccgtatgcct
     9961 gagccttcag gcgctccctc cagagagtgg agagagtggt gggagcacgg cgttgtaaag
    10021 aagccagaga ccatcaacta tagaacttta aagccggaga aggacggtct gttctgcgag
    10081 aggatttttg gtccaagcaa ggactgggag tgccactgcg gcaagtacaa aaagatccgc
    10141 tataagggcg tgatctgcga ccgctgcggc gttgaggtga caaaggccag tgtaagaaga
    10201 gagcgtatgg gacgtatcga gcttgcagct cctgtttccc atatctggta ctttaaggga
    10261 attcccagcc gtatgggact gattctggat atttccccca gaaccctgga gaaggtgctc
    10321 tactttgctt cctatgttgt actggatcca ggtgttacca gccttcagta taagcaggtt
    10381 ttgtctgaga aggagtaccg tgaggaagta gagaagtacg gcggctcaac tgccttccgt
    10441 gtgggaatgg gtgctgaggc gattcaggag cttctgatgt ccattgatct ggagaaggat
    10501 tcagctgatt taagaaagca gctggtagac gctaccggac agaagcgcgc aagaatcatt
    10561 aagcgcctgg aagtggtaga ggcattttta aactctggaa accgtccaga gtggatgatc
    10621 atggacgtaa tccccgtgat tccgccggat atacgtccta tggtacagct ggacggcgga
    10681 cgttttgcta cctctgactt aaatgacctg tacagaagga tcatcaaccg taacaaccgt
    10741 cttgccagac ttctggaact gggcgctccg gatatcatcg tccgcaacga gaagcgtatg
    10801 ctccaggagg cagtagacgc cttgatcgac aatggccgcc ggggccgccc tgtaacagga
    10861 cctggaaatc gtgcgcttaa gtccctttct gatatgttaa agggtaagca gggacgcttc
    10921 cgtcagaacc tgttaggtaa gcgtgttgac tactccggac gttccgttat cgtagtaggc
    10981 cctgaattaa agatttatca gtgtggtctg ccaaaggaaa tggctattga gctgtttaag
    11041 cctttcgtta tgaaggagct tgtttccaat ggcaccgctc acaatatcaa gaacgcaaag
    11101 aagatggtgg agaagctcca gacggaggtt tgggatgtac tggaggatgt aattaaagag
    11161 catcctgtta tgctgaaccg tgctcctacg ctgcacaggc tgggtatcca ggcttttgaa
    11221 ccgatcctgg tagaaggtaa ggccattaag cttcatcctc ttgtttgtac cgcgttcaac
    11281 gctgacttcg acggcgacca gatggcagtt catcttcctc tgtctgtaga agcacaggct
    11341 gagtgccgct tcctgctttt aagcccgaac aacctgttaa agccttctga cggcggccct
    11401 gtggctgttc cgtctcagga tatggttctc ggtatttact acctgaccca ggagcgtccc
    11461 ggtgcaaagg gcgagggaat gattttcaag agtgtaaatg aggcaattct ggcttatgaa
    11521 aaccatgaag ctactctgca ctccagggta aaggtaagag tatcaaagac tctgcctgac
    11581 ggcacggtaa aaactggtat tatcgagtct accatcggcc gtctgatctt caacgagatc
    11641 attcctcagg atcttggctt tgtagaccgt tccattcctg aaaatgagtt aaagctggag
    11701 gttgacttcc tggtagctaa aaagcagtta aagcagatcc ttgagaaggt tatcaacgta
    11761 cacggcgcta cccagacggc cgttactctg gatgacatca aggcaatcgg ctacaagttc
    11821 tctaccagag cagccatgac cgtatccgtt tccgatatga ccgtgcctgc aagcaaaccg
    11881 gaactgatcg caaatgcaca ggctactgta gaccacattg caaagaacta ccgccgcggt
    11941 ctgatcaccg aggaggagcg ctataaagaa gtaatcgaaa cctggaagac caccgatgat
    12001 cagctgaccc acgatctgct gaccggactg gataagtaca acaacatttt catgatggct
    12061 gactccggtg cgcgaggatc tgataagcag atcaagcagc tggcaggtat gcgaggactg
    12121 atggccgata caactggtca caccatcgaa cttcctatca agtctaactt ccgtgaaggt
    12181 cttgacgtac tggagtactt catttccgct catggtgcac gtaagggtct gtctgatacg
    12241 gctcttcgta cggctgactc cggttacctg accagacgtc tggtagacgt atctcaggat
    12301 ctgatcatcc gtgaggtaga ctgctgtgag ggcaaggata ttccatttat ggagattaag
    12361 gcattcacag acggaaatga agtaatcgag gatctgcagg agagaattac aggacgttat
    12421 attgccgaag atatcgttga tcccgatacg ggagagctgg taatgaaggc aaaccatatg
    12481 tgtacgccta agcgtgctgc agctgttatg aaggtattgg ataagcaggg acgcaagtct
    12541 gtgaagatcc gtaccgtatt aagctgtaag tcccatatcg gtgtgtgcgc caagtgctac
    12601 ggagccaaca tggcaaccgg ccagccggta caggttggtg aggcagtcgg aatcatcgcg
    12661 gcacagtcca tcggtgagcc cggtacacag cttaccatgc gtactttcca tacgggcggc
    12721 gttgccggcg gcgatattac acagggtctt cctcgagttg aggagctttt tgaggcccgt
    12781 aagccaaagg gtcttgccat tatcactgag tttggcggcg ttgtaactat caaggataca
    12841 aagaagaagc gtgagatcac agttactgac aatgaaaccg gaaattccaa gacctatctg
    12901 attccttacg gttccagaat caaggttatg gacggacagg ttcttgaggc cggtgatgag
    12961 ctgaccgaag gtagcgtaaa tcctcacgat atcttaaaga tcaagggcgt tcgtgccgta
    13021 caggattaca tgatccagga agtacagcgt gtataccgtc ttcagggtgt agaaatcaac
    13081 gataagcacg ttgaaatgat cgttcatcag atgttaaaga agatccgcat cgaggaaagc
    13141 ggcgacagtg atgtactgcc gggcgtatcc atggatgttc tggatttcaa tgagatgaat
    13201 gaggctctga tcgcagaggg caagcgtccc gcagagggca agcaggttat gctgggtatt
    13261 acaaaggcat ctcttgctac agattccttc ctgtcagccg catccttcca ggagaccacc
    13321 aaggttctga ccgaggccgc tatcaacggt aaggtagatc acctgattgg tctgaaggag
    13381 aatgttatta tcggtaagcc aatcccggca ggtaccggta tgaagcgcta cagaactgta
    13441 aagttggata cggatgataa gatcgcagcg gcagctgagg aagagatcct tctttctgaa
    13501 gaggaagagc tgacatctga ggaaagcagc gagcttacag aagataatgt agcagaagag
    13561 gttctggatc tggacgatac tgtaatcgag gacgaggatg cagaggaaga agaaagctct
    13621 gaggatgcag aagaataaaa tacaggtctg aatgacagat tgaatcataa aaacaccgga
    13681 cggacaggga aaactggcca tccggtgttt tttatgcatt ttgtgttctg agccattcgg
    13741 cccagaccgt ataatacttt cccataatgt gggcattgtc tacagaatat ttggaatcaa
    13801 ggtttccctg gccgtcgtca aaaagactgt ttacatctat atagccgcag cctttttcta
    13861 cagccatctg gtaaatagct tgatttaaag aatcaatttt tccatttcca tagaccgggt
    13921 cggaatcgga cttctttttt gttacatgga gattagcctc cagaatgatt ctggcctggg
    13981 gctgaagctg gtggatccag tctacgatag aggcgtactt ctttgttacg gaggaaggag
    14041 ggtatcccag ttcattgatg cccagcatga ggtaaatccg ctcatagctg tgttgggaga
    14101 gaagttcttc cagtgttttt ttgcttccat cctttacggt aagattggtt gagagaaggt
    14161 taaagctgct catgccggaa ttggcaaaaa actctgcttc ttccagacct ccgtattcct
    14221 ttaagccttc tgtccgtgaa tctccgataa aaagggaggt gcttaggtca acgggaacag
    14281 aggtgtcagc aggcggcaaa gtctccgccg gaggcgcttc tgtggaggac tctgtgacag
    14341 gggcagaccc gtcagtgggc ggctccgcag gggaggactc tggagagggg aactttgctg
    14401 taggagtttc ggtgctgcct ttggaggcag cactttcgga aatggaatca gaggataggg
    14461 ggatgttaga tggctgttta tcctcagttt ctgccagccg ctcgatttta tgttcacctg
    14521 aaaacgtttc tagttcccat atccggggaa taagggaact tccttttacg gcaatcaaac
    14581 ttccaataag catcatagta taccagtatt ttttcatttt attcacacaa tttctctttc
    14641 tatctgtttc ttagaacttt ccttagaacc ggaagtataa aaacgggtca tccattccca
    14701 ttttaataca gtaaacacag agccagaaca gaactataag tataacggca gtcagaaaac
    14761 tgcggcggtg ccggctgtag atccgccttg gcaggtcggt cataaaaatc aggcctgcaa
    14821 gaagagacaa cccatagatg cggccgtatt tcagatagtc tccctcaaac caggtgtagg
    14881 ttccgggggc tgtgaaaaac ggaaagagcc tctgtaaata aataaggatc tgtggcggct
    14941 gtgaaatggc aaagaccagc cagtttaaag gaatcacaag agccatatac aggtgccccg
    15001 cggctggaaa acggtttaag atacgtccca gtcccatttt ttcaatagag atcagagcaa
    15061 aaagcagaag accccagaga agaaaattcc agtcagcgcc atgccagaaa ccagtcagaa
    15121 gccacacggt cagtagattg cgcaccataa tgacaaagcc ttttcgattg ccaccaagag
    15181 gaatatatac atagtcgcgg aaccattttc caagggttat atgccaccga cgccagaact
    15241 ccgtcattgt cagagactgg tagggatagg caaagtttct gggaatctga aatcccagga
    15301 tccagccgat tccctgggcc atgagggagt agccgtaaaa gtcaaagtaa agctgaaggc
    15361 tgaatgcaat aattcccatc caggcaagcg gcgtggagat gctttcgtat ccaatggctc
    15421 cgacctggct ccacagcctg ccgagctgat tggcaagcag caccttaagg cttaagccaa
    15481 tagtaaactc tctaagaccg gtttccagac ggtccatgga aaaacggcgc cggatcagct
    15541 gagagcggat ttcctgataa cgggtaatgg ggccggaggt tacctgggta aacatggaaa
    15601 accaggtaca gaagcgaagg agggaatgtt cactgcggat ctccttctta tatacatcgg
    15661 ccagataggc gatggcctga aaggtgtaaa agctgattcc aagggggagg gacaggcgga
    15721 tgacgggaaa ctgcagacct gtttttgtat atgataggag caggtttaag ttttccgtta
    15781 taaatgtgct gtatttaaaa aggatcagcc agaacaggtt atagaagatc ccggcattca
    15841 gccagaagct gcgttctttt gcatcccgtt ttcttccaat aagctgtccg atgaaaaagt
    15901 ttacaggtat tgaaaggaga aacaggacaa aataaagagg ggagtgtctg gctccataaa
    15961 aatagaagat caggcttccg gcaaaaaggc actgattccg ccatttctcc ggacatatga
    16021 aataaagaat cagaaataaa ggaagaaaac gaaataaaaa gtcaaagctg gaaaatatca
    16081 ttagtagtcc atcctgtcta aatcatattt aagtttagga tgcaaatggc ttctgcacct
    16141 catataatat aaattagacg aacggaagca tataaaagtt tcatagaaac agaaaaaatt
    16201 taagaaagtg ggaacacatg aaaaaagccg caggcttctg cgtcagcatt tgccatgcgg
    16261 ctttgagccg gggatggtca ctgtcttaca gagaatggat tagtcgtccc agtggtcatc
    16321 atcatcgtcg aatctgtcgt catcgtcgaa tctgtcgtca tcccagcggt cgtcatcatc
    16381 ccagcggtcg tcatcatccc agctgttatc gcggtcgtca tcccaacagt catcccgcag
    16441 attatcccag tcacggttgt tttcgtaatc agccttgatc actttaccgg tataagcgtt
    16501 tacttccaca tcatactcaa actttccggc agtgaattcc agctcatata cgccgtcgtc
    16561 cagttcgcat tcctgttctg tgaacacagc atccgcttct ttaattttca tagcctttaa
    16621 agcggcttcc tttgcctgct caatgccgat tatctttttg gcagttctgg gggatgctga
    16681 tgcggtaatc gtgctgccga ttacagttac aagtgccatt gctgcaacaa ctgctgccat
    16741 catagctttt ttcaggttca tctttctcat tatagtttcc tccttgatct atcacctgtg
    16801 cttggattgt tttttcttta catagacaga atacggagtg catttgaaaa aaacggggtc
    16861 gaaatgaaaa aaatataatt tttttaaaag tttaatatgt ccctgatatc aggtcgattt
    16921 taatcattgc tgtcatcatc tgctgcgtca tcccagtcgt cctcctgatt atcgtctgag
    16981 ccatgattgg tggtatcatc ccaatcctca tcccgctgtt catctgaatc ctcattccaa
    17041 ttatcattcg ggtgggctgt cgggacagtt tgatgtgccg ccggggcagt ctgcacagcg
    17101 gcaggcactg tttcggaaac ggggggagaa gcaggaaatg cggtttcggc ttcacgtgcc
    17161 gtaccagatt ccggactctc atcaggtttg ggatcagcac ttatatggat atccgatcca
    17221 aggcgaccgt caagctccca ggtgagatcc cttttcatct catccgccca gtcctcatga
    17281 gcagaggaaa cgcctacata gatcgtcccg cctggagcca gatagtccat atctatggcc
    17341 cgctcggcca gacctgccgc agcttcttcg actttcttcc ttttatagtc atatccccgg
    17401 atcaagtcct ctccatcccg gttgatgccg gataatgaaa gcacataatc cagccggttt
    17461 acagacagct ctacttccgg gttgatggac agccgtactg ttccgtaagg gctcataata
    17521 aaatagtggc ctccagacag cagaaggcaa agggctgcgg ctgcgggaag agcagacttt
    17581 aaccagcggg aaaggcggct ggtccttcgg acggcaggct gtacttccac cgcttcctca
    17641 aatactgtat cgtaaaaaga atcagactgg cagtccgctt ccgaaaaggc ggctccgggt
    17701 tctacgattt ccataatggt atctaccgtc tgtcctacct cataatccat attggcggcc
    17761 ttcagaaaac gcccgtcctc atccagcacc actgcataag ccatatgaca ttccatgatc
    17821 agatatttca ttctgctgcg cctccttttt tcatctgtga gagatgaccg cgtatgattt
    17881 caaatccgtt tgtaaaagca agaagaatgg caatgagata tttccggtga cgttccagcg
    17941 tctttttttc tgcccctgag cccagagcca gctggcttaa ggggagttta cggctgcgga
    18001 tcatctgttc cagaagctcc ggatgttttt ttgcgtaatc cagaaccttc atgcaggcgt
    18061 ccagggtgcg ctcctggcgg gggcagctgt ctgccacatc agtgagcgaa agtccaaaac
    18121 ctgcaagctc tgcggaaaat gtttcgattt ccgactgagc ggcagccaga tcctgataat
    18181 cggaaatatc atcaactccc ggatcggtct tttccaaaag agtgcagccg tcctcctccg
    18241 caggctggtc taaggagagc tgcccactgt gcttttgctc cttccggaaa tagtcgatca
    18301 ggcggtttcg gattccggct gctgcaagag gcagaaaagg acctctgtcc ggattataaa
    18361 ccgtcattgt ttcataaaaa gcaaacatgg ccacagacag ctcatcatcc cgtccctcaa
    18421 ccggaggacg gtggataaag cgtgcggttt ctgctttgat aaatggcata taggatcgga
    18481 tcagctgatc cgctgcctca ggatcctgcc tggagcgcat gacctgctga acgattttat
    18541 gttcctcgaa tttcatagta atcctcccaa agatagaata cgcagacaaa gaaatgaaaa
    18601 acggggcagg ggctgcgtat atttgattat ccatgaaaat accagatgat gcaataaaaa
    18661 ttgcgtaaat gttttataga aaaatgcaaa agagcaccct caaaaaatac ttgacagatt
    18721 tacgccttat gctataatga aaaagtgtgt tcaaaacact tatgttttgg tgcacaaaag
    18781 ctgaaagaca cagcaaccaa cagtcaatat cacaggaggt gaaaaggatg ccaacattta
    18841 accagttagt tagaaaggga agacagagca gcgcaaagaa gtctactgca ccagctctgc
    18901 agaagggtta caactcttta cagaagaagg caacagacgt atctgcacca cagaagcgcg
    18961 gcgtatgtac cgctgttaag actgctaccc ctaagaagcc taactctgca cttagaaaga
    19021 tcgccagagt acgtctttcc aatggtatcg aagtaacaag ctacatccca ggagagggac
    19081 acaaccttca ggagcacagc gttgttctga tccgtggagg aagagttaag gacctgccag
    19141 gtaccagata ccacatcgtt agaggtacac tggatacagc aggcgtagca aacagaaagc
    19201 aggctcgttc caagtacggc gctaagagac cgaaagacaa gaagtaattt caaagcagtt
    19261 tggaacaaac cagattagta ccactaacag cagtaaagtg acggcggaag atatttccgc
    19321 atttgttagt gccggatccg tgatattgta cctgtggttg caggaaatat atagaccgag
    19381 cacgaacaca ttttatggaa acatgtgagt accgttgagt taatggaaac tgcctgtatc
    19441 atttcagata aaaggcagca tattaaggag ggaagtaacg tgccacgtaa aggacatact
    19501 cagaaaagag acgttctggc agacccgatc tacaacaata aggttgtgac aaagctgatc
    19561 aacaatatca tgttagacgg caagaagggt gttgcacaga aaatcgtata cggcgcattc
    19621 aatcgtgtag ctgaaagcac cggtaaggat gcaatcgaag tatttgaaga agcaatgaac
    19681 aacattatgc ctgtacttga ggttaaggca aagcgtatcg gcggcgctac ctatcaggtg
    19741 cccatcgagg ttaagcctga gagaagacag gctctggctc tgcgctggat cactctgtac
    19801 tcccgtaaga gaggcgagaa gactcaggaa gagagactgg caaatgagat tatggacgca
    19861 gccaacaaca ccggcgcatc tgtaaagaag aaagaggata tgcacaagat ggcagaggca
    19921 aacaaggcgt ttgctcacta ccgtttctaa ttgcagaccg gcttttttcg ttaaattaag
    19981 gaggaaaatc ccttggctgg aagagaatat ccgttggaaa gaaccagaaa tatcggaatc
    20041 atggctcata tcgatgctgg taagactacc acaaccgagc gtattctgta ttacactggt
    20101 gtaaattata agatcggtga tactcacgaa ggtactgcta ccatggactg gatggctcag
    20161 gagcaggaaa gaggtattac cattacttcc gctgctacaa cctgccactg gactctgcag
    20221 gaaaactgca agccgaagcc aggcgcatta gagcaccgta tcaacatcat cgatacacct
    20281 ggacacgtag acttcactgt tgaggttgag cgttccctgc gtgtattgga tggcgctgta
    20341 ggtgtgttct gtgcaaaggg cggcgttgag ccacagtctg agaacgtatg gcgtcaggct
    20401 gacacttaca atgtaccccg tatggcattc atcaacaaga tggatatcct gggtgcaaac
    20461 ttctacggcg ctgtagagca gatcaagacc cgtctgggca agaacgctat ctgcattcag
    20521 ctgccaatcg gcaaggaaga ggatttcaag ggaatcgtcg atctgtttga gatgaaggcc
    20581 tacatctata atgatgataa gggcgatgac atttctatcg ttgacattcc tgaagatatg
    20641 caggacgatg cagagttata ccgtaccgag ctggtagaga aaatctgcga gctggacgat
    20701 gacctgatga tgatgtatct tgagggcgag gagccttcca atgatgagct gaagaaggta
    20761 ttaagaaagg ctacctgcga gtgtacagca gttcctgtat gctgcggtac cgcttacaga
    20821 aacaagggcg ttcagaagct tctggatgct attatcgaat tcatgccatc tccattagac
    20881 atccctgcga tcaagggtgt tgacctggac ggaaatgaga ttgttcgtca ttcttctgat
    20941 gaagagccgt tcgcagcgct tgcattcaag attatgactg acccattcgt gggcaagctt
    21001 gcattcttcc gtgtatattc cggtactctg aactccggtt cttacgtact gaatgctaca
    21061 aagggcaaga aggagcgtgt aggacgtatc cttcagatgc atgctaataa gagaaatgaa
    21121 cttgataagg tttactccgg cgatatcgct gcagcagtag gctttaagac aaccggtaca
    21181 ggcgatacca tctgtgatga gaagaaccct gttatcctgg agtctatgga gttcccagag
    21241 ccggttatcg atatcgcgat cgagccaaag acaaaggcaa gccaggacaa gatgggcgac
    21301 gctttagtca agctggcaga agaagatcct actttcaagg ttcgtacaga cgaagagacc
    21361 ggccagacca ttatctccgg tatgggcgag cttcatctgg atattatcgt agaccgtctg
    21421 ctgcgtgagt tcaacgtaga ggctaacgta ggcgctcctc aggttgctta caaggagacc
    21481 tttaccaagg ccgttgacgt tgacagcaag tatgcaaagc agtctggtgg tcgtggacag
    21541 tacggtcact gtaaggttca cttcacacca atggaagcaa acgcagaaga gaccttcaag
    21601 tttacttcat ccgttgttgg cggtgctatt cctaaggagt acatcccggc agtcggcgca
    21661 ggtattgagg aagcagctaa gactggtgtt ctgggcggat tccctgtact gggcgtttgg
    21721 gcagatgtat acgacggttc ttaccatgag gttgactctt ctgaaatggc attccacatc
    21781 gcaggttcca tggcattcaa ggatgctatg cagaagggta atccaatcct gcttgagccg
    21841 atcatgaagg tagaggttac catgcctgag gagtacatgg gtgatgttat cggtgatatc
    21901 aattcacgcc gtggacgtat cgagggtatg gaggacattg gcggcggtaa gatggttaag
    21961 gcttacgttc cgctgtctga gatgttcggc tactctacag accttcgttc cagaacacag
    22021 ggacgcggca actactccat gttcttcgag aagtatgagc cagttccaaa gaacgttcag
    22081 gagaaggttc ttgctgacca caaaaagtaa aaaaggcttg taaatcaaag taaaatagaa
    22141 tataatgatt gatagcctga ctaactatat cagccctaaa aggggcgcaa attaaggagg
    22201 acgttataaa atggctaaag ctaagtttga cagaactaaa ccacattgta acatcggtac
    22261 cattggacac gttgaccacg gcaaaactac tctgaccgca gctatcacaa aggttctttc
    22321 agagagagtt gcaggcaacg caaaggtaga tttcgagaac atcgataagg ctccagagga
    22381 gagagagcgt ggtatcacca tctctacttc tcacgttgag tatcagacag agaagagaca
    22441 ctatgcacac gttgactgcc caggacatgc tgactacgtt aagaacatga tcactggtgc
    22501 tgctcagatg gacggcgcta tcctggttgt agctgcaact gatggtgtta tggctcagac
//
LOCUS       DBMDUH010000026        22223 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_1506_length_22223_cov_4.253820, whole genome shotgun sequence.
ACCESSION   DBMDUH010000026 DBMDUH010000000
VERSION     DBMDUH010000026.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 22223)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 22223)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22223
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1506_length_22223_cov_4.253820"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..159)
                     /locus_tag="ACZPCG_03830"
     CDS             complement(<1..159)
                     /locus_tag="ACZPCG_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR1284237.1"
                     /translation="MSTLSYHEQKDKENIIRLRSLIRDLPGFCADFFRGIEPRTSSRT
                     RIAYAYDLH"
     gene            complement(436..693)
                     /locus_tag="ACZPCG_03835"
     CDS             complement(436..693)
                     /locus_tag="ACZPCG_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HIR1284238.1"
                     /translation="MISYRYVISNRQGLHASNSMSLSRAASDYQSQITISSAKGEANC
                     KNVLALMSLNARQGENLVLKIDGPDEKQAAGFLNGLLRTIL"
     gene            923..1660
                     /locus_tag="ACZPCG_03840"
     CDS             923..1660
                     /locus_tag="ACZPCG_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006780554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis TtcA family
                     protein"
                     /protein_id="HIR1284239.1"
                     /translation="MKLQRLLSLIRQALDTYHMIQDGDHIAVGISGGKDSLALLYALH
                     HLRRFYPQKYDLTAITVDLGLGNLNLDPIRELCKEFEIPYYVLPTEIGTVLFDIRKES
                     NPCSLCAKMRKGALNEKAKELGCNKIAYAHHKEDVMETMMMSLFYEGRFYCFPPNTYL
                     DRMDLTVIRPMIFVSEADVIGFKNKYQLPVCKNPCPMDGYTKRQYIKELIQKLQKDNP
                     HIKDCLFRAITDGNIPGWSKAADSADA"
     gene            complement(1657..3465)
                     /locus_tag="ACZPCG_03845"
     CDS             complement(1657..3465)
                     /locus_tag="ACZPCG_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006780564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIR1284240.1"
                     /translation="MNYHDNEDELLRMQSRRRRAGSYGNVPSRSRASSHDGRSFRERE
                     SFDSIDDAYFEDEYGCEDGLDIPFSDESSKRGTSQGRSAKRAGGFSKNHRESYSRSRN
                     KFRTGEKSRRNPKGSSVRERRRKRKKKWPMLLIFLLLAVTAYGSFLYLHRPTGIWTVA
                     VFGVDSRDGNTKKALADVQMVCTLDRETGEIRLASVFRDTYLKIDSKGTYHKINEAYF
                     KGGQKQAVDALEENLDLKIDDYAAFNWKAVAEAINVLGGIDLEITPAEFKYINGFITE
                     TVNSTGIGSTQLKQAGANHLDGVQAVAYSRLRLMDTDFQRTERQRKVIALALEKARQA
                     DAGTLTNLAKAILPQISTSVGIEDVLPLVKDIDQLHIADTAGFPFSRETKRVGKMDCV
                     IATTLESNVIQLHQFLYGEDTPYHPSSGLKKISAHISEETGLYEEGAAAPSKSSSGSG
                     KIDSKDKTGGADAPKETEASKPQETIPETSAEETETEEESSSLEKTEETRETISIKES
                     EKETTKEEEKRPGIWGSGSSKETESEKETGSLENKLSEEKEEEDTSRLLGEAGSTGPG
                     ASSDQQPGGGPGISALPGASKDTKPGESVSNQGPGI"
     gene            complement(3694..4332)
                     /locus_tag="ACZPCG_03850"
     CDS             complement(3694..4332)
                     /locus_tag="ACZPCG_03850"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HIR1284241.1"
                     /translation="MSGNLVITIGRQCGSGGKLIGEKLAEKMGVKCYDKELLSKAAKH
                     SGLCQELFEKHDERPTSSFLYSLVMDSYSMGYTAAGYSDMPINHKIFLAQFDAIKHLA
                     DEQSCVIIGRCADYALEDYPNVLNVFIAGDEKDKLERIKMLYNLDDSKAKEVIVKTDK
                     KRSSYYNYYSNKRWGDPRSYDLCLNSSSVGPDGAVEAIMAFAKLKQQWKDQK"
     gene            complement(4506..5417)
                     /locus_tag="ACZPCG_03855"
     CDS             complement(4506..5417)
                     /locus_tag="ACZPCG_03855"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR1284242.1"
                     /translation="MKQEICVGDQEGGLRIDKYLAEAAGQLSRSYIQKLLKDGRILVN
                     GKPVKASYVVEDEDILSMDIPEAVEPEIEPENMELDILYEDQDVILINKPKGMVVHPA
                     AGHYSHTLVNGLMDHCRGQLSGINGVMRPGIVHRIDMDTTGVLIVCKNDMAHNSIAAQ
                     LKEHSITRRYQAIVHGVIKEEEGTIDAPIGRHPVERKKMSINYDKGKLAVTHYRVLKR
                     FRQFTHVECRLETGRTHQIRVHMASIHHPLLGDAVYGPAKCPISGLKGQTLHAGVLGF
                     IHPRTGLYMEFSAPLPDYFENLLKTLN"
     gene            complement(5414..5944)
                     /gene="lspA"
                     /locus_tag="ACZPCG_03860"
     CDS             complement(5414..5944)
                     /gene="lspA"
                     /locus_tag="ACZPCG_03860"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HIR1284243.1"
                     /translation="MSRQKRSLLVSFLIGFLILVGLDQWTKGLAVRFLKNQEPVAVIK
                     GVFEFSYLENRGAAFGMLQNRQFFFFLIGIAVLAAILYMMWRMPETPKYRPLAVCLML
                     VAAGAVGNMIDRMTQNYVVDFLYFRLIDFPVFNVADCYVTIAAAGLVLLVLFYYDDED
                     LDCFQPGKAHGKEKTQ"
     gene            complement(6002..7090)
                     /gene="aroB"
                     /locus_tag="ACZPCG_03865"
     CDS             complement(6002..7090)
                     /gene="aroB"
                     /locus_tag="ACZPCG_03865"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HIR1284244.1"
                     /translation="MKERLTVCRDGDPIYDICMETSFEGLKGELAAFHLEKRKICIVT
                     DSNVAFLYLDKVKEILSQCCSMVSVFIFPAGEEHKNLDTVRMLYEHLILEHFDRKDML
                     AALGGGVTGDLCGFAAATYLRGIDFIQIPTTLLSQVDSSIGGKTGVDFADYKNMVGAF
                     HMPRLVYTNLETLLTLPAEQFSSGMGEVIKHGLIQDKAYYEWLKEHRDAVMARDLTTC
                     RQMVFRSNEIKKAVVEKDPTEQGERAYLNFGHTLGHAVEKLKNFTMLHGHCVGLGCVA
                     AARISCLKGTLSETEADDIKHTMASFELPTTVDGLIWKEVFDATKSDKKMEAGRVKFV
                     LLNGIGDAYVDHSLSEDEIRAGFEAVSL"
     gene            complement(7212..7733)
                     /locus_tag="ACZPCG_03870"
     CDS             complement(7212..7733)
                     /locus_tag="ACZPCG_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HIR1284245.1"
                     /translation="MSLLGKLMDTMRLNPEEEGEDYYLDDDFEDEPPKKSFFSRKNTA
                     DDEEYYDEPQDKSRFLGRPTPKVVPMRRSGMEVTMVKPTSMDSSKDICNYLLSGKAVV
                     LNMEGIHMEVAQRIIDFVSGAAYSMDGNLQRISNYIFIVTPNSVELSGDFQDMLAAGD
                     VSGMEMAGMTIHI"
     gene            complement(7769..8470)
                     /locus_tag="ACZPCG_03875"
     CDS             complement(7769..8470)
                     /locus_tag="ACZPCG_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HIR1284246.1"
                     /translation="MSEIRDNLEIVRRRIEEACQRAGRDASEVTLIAVSKTKPVPMLE
                     EAYEGGARDFGENKVQEIQAKYGSLPDDVRWHMIGHLQRNKVHQVLGKAVLIHSVDSL
                     RLARQIEEEAAKKNIEVDILLEINVAKEESKYGFFMEDAEAAIREISTFPHVHIRGLM
                     TIAPFVENPEENREIFKKLYEFAVDIGRKNIDNVTMDVLSMGMTGDYEVAVEEGATMV
                     RVGTGIFGERHYGAV"
     gene            complement(8514..9920)
                     /locus_tag="ACZPCG_03880"
     CDS             complement(8514..9920)
                     /locus_tag="ACZPCG_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HIR1284247.1"
                     /translation="MASKKHRKNRKIIPYRRPRNDIGKLIFGVIFLYLLINIFIYMGH
                     EKIQFYEVAEGGIVNDRPYTGLILREEQVYNADNSGYINYYLREGKRASVGSRVYSLD
                     ETGRLDSLLKEHGVENQTLSDENLADLKKQLSSFVTSRSDEDFGAIYDARYTLEAAVM
                     EYSSFSALDQLDQIARESGIVFEQVVSPQSGVVSYGFDSYESIKPEEVETASFDRSAY
                     TKTFHKSGDLIESGTPAYKIATSENWSVVFPLTEEDRALYGDKSSLEVEFKDHSIETT
                     ASFSIFTGKDGSVFGKLDFNKYMAQFITDRFVNFEIRQDQAKGLKIPVSAVTEKNFYQ
                     IPRDFVTQGGDSTDSGVNKEIYDANGTTAVFTPIEIGFDDEEFYYIAVQEDGPLKAGD
                     YIVKPDSQERFQVGSTRALKGVFNINKGYTVFKEIEILDSNSEYYTIKKGASYGLSVY
                     DHIVLDASLVTEGQILYQ"
     gene            complement(10048..10467)
                     /locus_tag="ACZPCG_03885"
     CDS             complement(10048..10467)
                     /locus_tag="ACZPCG_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility protein PilT"
                     /protein_id="HIR1284248.1"
                     /translation="MVQIIAGKKGKGKTKHLLDMANAAIKGASGTVVYLDKSAQHMYE
                     LNNRIRLINVNEFPITHSEGFLGFICGILSQDHDLETMYLDSFLKLACLEGADISETY
                     ETLKKLSDKYHVTFVLSISMDADELPENAKGDVIISL"
     gene            complement(10557..11528)
                     /locus_tag="ACZPCG_03890"
     CDS             complement(10557..11528)
                     /locus_tag="ACZPCG_03890"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003504020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR1284249.1"
                     /translation="MKRGYVRAWSALILAASLCLGGCFGGFSDAYSLKKRLPAIALAG
                     SEEEVMEGFASDLCVVSDESVFDPDFLTSEAGALFDLNGHQTLYSKDAFETMYPASIT
                     KVMTALVAIKYGNLEDQVKVTDEAVITESGATLCGIKPGDTLTLEQLLYGLMLPSGND
                     AGVAIAVHMDGSIEAFADRMNEEAKRLGAVDSHFVNPHGLHDEDHYTTVYDLYLIFSE
                     AFKYPKFREIIEAKAYTADYINGAGESVSRTWKCSNQFITGERDTPEGVTVLGGKTGT
                     TKAAGYCLIIASKDDASEGNFISVVLKSDSRAHLYDNMTNIISKIVK"
     gene            complement(11528..11923)
                     /gene="mgsA"
                     /locus_tag="ACZPCG_03895"
     CDS             complement(11528..11923)
                     /gene="mgsA"
                     /locus_tag="ACZPCG_03895"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HIR1284250.1"
                     /translation="MTIGLIAHDAKKKLMQNFCIAYRGILSKHSLYATGTTGRLIEEV
                     ANLNIHKHLAGHLGGIQQMAAQIEHNDMDLVIFLRDPLMPKSHEPDVNDVVRLCDTYN
                     IPLATNLATAELLIKALDRGDMEWREVDR"
     gene            complement(11945..13066)
                     /locus_tag="ACZPCG_03900"
     CDS             complement(11945..13066)
                     /locus_tag="ACZPCG_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006774756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIR1284251.1"
                     /translation="MLFDYDFRNYSFRLLAYMTALSVIGILVVRSATNMDSEAVTKQM
                     FGILAGMAIAVCLSLVDYHKILNFSTVIYLICIGSLLAVLFWGRSVNNAKRWIEVPVL
                     GQLQPSEFVKIGLIITFSWYFMKYQEKVDKVSTVVLAAALFAVPAMLIFEQPNLSTCL
                     VTVVIVLGIVFASGISYRWIVGTLAVVIPMIATGVYLLLHGMIPFIKEYQAGRILAWF
                     YPEQYGEARYQQNNSIIAIGSGQLHGKGLYNTTIASVKNGNFLSEEQTDFIFAVIGEE
                     LGFIGCAIVILLFLLIVYECLMMAARAKDMAGRLLCTGMATLIAFQSFANIAVATGIF
                     PNTGLPLPFISFGSSSLISIFIGMGLVLNVGLQRVPRHY"
     gene            complement(13068..13325)
                     /gene="minE"
                     /locus_tag="ACZPCG_03905"
     CDS             complement(13068..13325)
                     /gene="minE"
                     /locus_tag="ACZPCG_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HIR1284252.1"
                     /translation="MKTRRSKDTAKFRLKLLLVADKAGCSPELIRLIKNDMIRVISKY
                     MEIEKDNVRLLMDTDGSASCRGLPVLQADIPIRSISNKGLY"
     gene            complement(13341..14132)
                     /gene="minD"
                     /locus_tag="ACZPCG_03910"
     CDS             complement(13341..14132)
                     /gene="minD"
                     /locus_tag="ACZPCG_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007718407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HIR1284253.1"
                     /translation="MSEVIVITSGKGGVGKTTTSANVGTGLAVLGKRVVLVDTDIGLR
                     NLDVVMGLENRIVYNLVDVVEGNCRMKQALIRDKRYPSLYLLPAAQTRDKSSVNPEQM
                     VKLTEDLKEEFDYILLDCPAGIEQGFYNAIAGAGRALVVTTPEVSAIRDADRIIGLLE
                     HAGITEIGLVVNRIRADMVRRGDMMSLEDVRDILAVDILGAIPDDENIVVSTNQGEPL
                     VGIGSAAGQAYLNVCRRLLGQNIPIESPGEARGFLVRLARVLKRA"
     gene            complement(14251..14913)
                     /locus_tag="ACZPCG_03915"
     CDS             complement(14251..14913)
                     /locus_tag="ACZPCG_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HIR1284254.1"
                     /translation="MQNGIVIKSSRAGMTVLLDPNLPFEELLAAIAKKFKDSARFWGA
                     VQMTLTLEGRRLTPEEEMAVVNTITENSQIDVLCLLDTDAQRIERCEKALNEKLMELS
                     AQTGQFYRGTLRRGDTLESESSIIVIGDVEHGARITAKGNVIVLGELKGSVTAGVSGN
                     AQAVVMAFSMMPFQLRIGNCPIPTNEKHRRFGRGAMIACIRDGEIAFRPVKGRSIFGD
                     MG"
     gene            complement(14939..17872)
                     /locus_tag="ACZPCG_03920"
     CDS             complement(14939..17872)
                     /locus_tag="ACZPCG_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009662593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HIR1284255.1"
                     /translation="MFDELKEVVKEFLKKLFSSRLFALAVIFTLMYAGLIGKLFNLQI
                     IHGDEYQQSYMQRTEKMVTAPGTRGNIYDRNGKLLAYNELAYSVTIQDLGDYPRPADR
                     NAMIYRLVTILERRGEKVEGKLEIAINEQGEMYFTTTSKASHKRFLLNFYGISSEKLD
                     DASGRYPSDITAREAFELKKSSGRDGYNLNDMKDEEGNPLELTDQTALDMINIIYTMK
                     LTEYQKYESTTVATNVSRETMTEIHENAADLKGVSVEESYIRVYNDSLYFSPIIGYTG
                     KVQEEQVDRLNEQWREEENKKGKNLTEEDEKYNLNDVVGRIGIEQYMELDLQGEKGYT
                     KMYVDNMGRPREVIEQQDAKAGNDVYLTIDRDLQIATYKLMEQQLAGIIIKFLVNEDV
                     DPATVKDGSKKPIPVKNAYYQLINNNVLSLEAMAEPDASSVENKIYSTYTSSKTQILT
                     DIKNELLSPHARAMNDLPEDMKSYMNYIYSFLASEETGIIKRDKIDTSSEEYLSWKEG
                     TISLRDYIYSGISGNWIDTTKLDSENKYSNADDSFAQLVDYICVHLEEDGKFTKRMFR
                     YLINNEVIRGSELCLALFDQGVLEPDPGAQAALAGGDSVYAYNFIKKKISNLELTPAQ
                     LALDPCTAGAVVTDVRTGEVLALVSYPSYDNNKMSGSVDAAYFAKLQNDMSNPLYNNA
                     TQAIKAPGSTFKPITAVAALEEGVISLTDTIECTGSYDLSSPPINCWVFPGRHGVEDI
                     VTGIQNSCNVVFAELGHRLSLTEDGTYSSEKGLEAIRKYASMFGLDHTSGIELPETEP
                     HLTTEDPERSAMGQGTNSYSNVQLSRYISAVANRGTVFELSLLDKLTDSDGNLIEDYT
                     PEISSQIEAKTSTWDAVQSGIRRVVTEGSAKSVFAGSHVEVAGKTGTAQEDRSRSNHA
                     FFVSFAPYSNPEIAVTVNIPYGYAGTNAAPLGKQIYEYYYGYTSFEEIVGSGALSASN
                     ISVEE"
     gene            complement(17865..18398)
                     /gene="mreD"
                     /locus_tag="ACZPCG_03925"
     CDS             complement(17865..18398)
                     /gene="mreD"
                     /locus_tag="ACZPCG_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HIR1284256.1"
                     /translation="MTSAKVKQIAINIGFIILAFTVQNCVFPLLPFLAATPNLLLILT
                     FSFGFIHGKNAGMFYGVISGLLLDLFYSGSFGFYTLIYIYIGYFNGIFTRYYYEDYIT
                     LPLILSVANGLWYCMYIYIFRFLIRGRLDLPYYFTKIMLPEIIFTAVTTLLIYRLFLS
                     ASRRVEDIGKRRDTTIV"
     gene            complement(18395..19240)
                     /gene="mreC"
                     /locus_tag="ACZPCG_03930"
     CDS             complement(18395..19240)
                     /gene="mreC"
                     /locus_tag="ACZPCG_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HIR1284257.1"
                     /translation="MESKRSKYILSGLTLLCVLLIGITSLKDGILEPLRTGVGYFLIP
                     IQTGVNRVGTGLYNELTDISRLKTALDENDRLKIQVDRLTEENNRLQAQQFELNRLRQ
                     LYELDQEYMQYNKIGARVIARDSEKWFQVFRINKGSSDGVAVDMNVVADGGLVGIVTD
                     VGANYATVRSIIDDSSRVGAMSVDSSYNCIVAGDLTLYEQGRLKLTDFSKDAVLKDGD
                     QIITSNISTKFLPGILIGYAVDVTIDPDHLTQSGYLIPAANFDNLQEVLILTDLKDGE
                     EVEKQ"
     gene            complement(19273..19968)
                     /gene="radC"
                     /locus_tag="ACZPCG_03935"
     CDS             complement(19273..19968)
                     /gene="radC"
                     /locus_tag="ACZPCG_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HIR1284258.1"
                     /translation="MERITMKDLPKEQRPYEKCMAYGPKALTDSELLAVIIRTGSSER
                     TSLELADAILSQNAPGDGLTGLLHCSLEQLQSIRGVGPVKGIQLLCIGELSRRIWKQK
                     VSAEPVAFTQPDQIADYYMEDMRHLEQEEIHVMFLNTKQHLIRDFLVSKGTVNASVVT
                     PREILIEGLRCLAVGMVLVHNHPSGDPEPSRSDRLLTCRIREAGEVVGITLIDHLIIG
                     DRRYLSFRREKLI"
     gene            complement(20018..20269)
                     /locus_tag="ACZPCG_03940"
     CDS             complement(20018..20269)
                     /locus_tag="ACZPCG_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4321 domain-containing protein"
                     /protein_id="HIR1284259.1"
                     /translation="MGRKNFWILLIMLLAGIVLGGFIGQLADGISWLKWMNYGQSFGL
                     DTPLVIDFGLLVITFGLTIKITMASIIGVAVALFIYKMI"
     gene            complement(20356..21660)
                     /locus_tag="ACZPCG_03945"
     CDS             complement(20356..21660)
                     /locus_tag="ACZPCG_03945"
                     /EC_number="4.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000096 - sulfur amino acid metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HIR1284260.1"
                     /translation="MEQNVLTDMYETLGISKRVLDFAAEIEEHLKTRFQEIDERAEFN
                     QLKVIHAMQECRVSDIHFAATTGYGYNDLGRDTLEEVYAHVFHTESALVRPQLMSGTH
                     ALHVALSGNLRPGDELLSPVGKPYDTLEEVIGIRDSVGSLKEYGVSYRQVDLLPDGTF
                     DYENIAKAVNEKTKLVTIQRSKGYDPRPTFSVKQIGELIAFIKKIKPEVICMVDNCYG
                     EFVETIEPTDVGADMIVGSLIKNPGGGLAPIGGYIAGRKDCVDRASYRLSAPGLGKEV
                     GASLGLNQQLYQGLFLSPTVVAGALKGAIFAANIYERLGYGVVPNGSESRHDIIQAIT
                     FGTPEGVISFCKGIQAAAPVDSFVTPEPWDMPGYDSPVIMAAGAFVQGSSIELSADGP
                     IKPPYAVYFQGGLTWYHAKLGILKSLQQLADDGVVSPKSLVS"
     gene            complement(21711..>22223)
                     /locus_tag="ACZPCG_03950"
     CDS             complement(21711..>22223)
                     /locus_tag="ACZPCG_03950"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-dimethylallyltransferase"
                     /protein_id="HIR1284261.1"
                     /translation="DPEAAAQIHPNNIKRVIRAIEYFRQTGEKISHHNEAMHQKESPY
                     HFLYYVLNTDRTRLYDRIDRRVDLMLEQGLIDEVQRLKEMGCHRGQTSMQGLGYKEIL
                     DYLEGRCSLEEAVYILKRDTRHFAKRQLTWFRRERQVRWIELSGAPDDTSNALKQILK
                     DCMDEGIVLQ"
BASE COUNT         5797 a         5661 c         4536 g         6229 t
ORIGIN      
        1 atgcagatca tatgcatatg caattcgggt tcgtgaagaa gtcctgggtt caattccacg
       61 gaagaaatct gcgcaaaatc ccggaagatc ccggatcaaa ctgcgaaggc gtataatgtt
      121 ttctttgtct ttttgttcgt gataggataa tgtactcata ataaaaactc catacaattc
      181 attttataaa aacagttgga gttatgtgat gagagacagt gtttaaacta ttcgcgaaac
      241 acagctttaa cgaatagttg aggcgggaat tactgatttt actaaaccat cgacttttac
      301 tgattttcag ctgcccctgc ttccttctgt tttatatcac atactcttca ttattgtcaa
      361 atgaataaaa cgtcccggac aatttttatc cgggacatat tcgtatctta catctgtgca
      421 gacaaactta ttttattata aaatcgtccg aagtaatcca tttaaaaatc cggcagcctg
      481 tttttcgtcc ggaccatcta tctttagtac aaggttttct ccctgtctgg cgttcagact
      541 cataagtgcc agcacatttt tgcagtttgc ctctcctttg gcgctggata tggtaatctg
      601 actctgataa tctgaagccg cgcggcttaa gctcatagaa ttcgatgcat gaagtccctg
      661 tctgtttgaa ataacatatc tgtagcttat cattttttat catcctttgc gttttagtgt
      721 ttaaattaaa tcttcaatat tacaggaata gacggcattg ttgtttaatt ttaatctttt
      781 atttatgaca ttgttggaaa tctctatcct tatagtaaca caattgtctg ttaatgacaa
      841 ccattgatta taaacacttg acaccggagg aacggacgat tagaataatt tggtacaaat
      901 ttatgaaaga aaggattata aaatgaaact ccagcgttta ttaagcttga tccgccaggc
      961 tctcgataca tatcatatga tacaggacgg agatcatatt gcagtgggaa tttcaggcgg
     1021 aaaagacagt cttgcactgc tctatgcgct tcaccattta agacggttct atccccaaaa
     1081 atacgatctt acagccatca ctgtagatct ggggcttgga aatcttaacc tggatccgat
     1141 aagggaactc tgcaaagaat ttgaaattcc ctattatgtg cttcccacag agattggaac
     1201 cgttctcttt gatatacgaa aggaatccaa tccctgttct ctctgtgcaa aaatgcgcaa
     1261 aggcgcctta aatgaaaaag ccaaagaact gggatgcaat aaaatcgcct atgcccatca
     1321 taaagaggat gtgatggaaa caatgatgat gtcccttttt tatgaaggac gcttttactg
     1381 ttttcctccc aacacctatc tggaccggat ggatctgact gtaatacgtc ctatgatttt
     1441 cgtatcggaa gcagatgtta tcggatttaa aaataagtac cagcttccag tatgtaaaaa
     1501 tccatgccct atggatggat acaccaaacg ccagtacatc aaagaactga tccagaaact
     1561 gcaaaaagac aacccccata ttaaggattg tctttttcgt gctattacag acggaaatat
     1621 acccggttgg agcaaagctg ccgattctgc agatgcttaa atccctggtc cctggttact
     1681 tacggactcc ccgggctttg tatctttaga ggctcccggt aaggcagaga taccggggcc
     1741 tccgcccggc tgctggtctg aggatgcccc aggtcctgta cttcctgctt ctcctaaaag
     1801 cctggaagtg tcctcttctt ccttttcctc agaaagctta ttttccagag atcccgtttc
     1861 tttctctgat tcggtttcct tactgctgcc cgatccccaa attcccggtc tcttctcttc
     1921 ctcttttgtt gtttcttttt cactttcctt tatactgatc gtttcccttg tttcttccgt
     1981 tttttctaag ctgctgctct cctcttctgt ttccgtttct tctgcagaag tctccggaat
     2041 tgtctcctgt ggctttgaag cctctgtttc ctttggagca tcggcgccgc ctgtcttatc
     2101 cttggaatct atttttccgg agcccgaaga agatttggag ggtgcagctg ctccctcttc
     2161 atacagccct gtttcttccg aaatgtgggc actgatcttt tttaatccgg aagatggatg
     2221 gtatggcgta tcctctccat acaggaactg atgcagctgg atcacattac tctccagagt
     2281 agttgcgatc acacagtcca tttttccaac ccgctttgtc tccctggaaa aaggaaaacc
     2341 tgccgtatct gcaatatgaa gttgatcaat atcttttacc agaggcagca catcttcaat
     2401 ccctacactg gtagaaatct gaggtaaaat agcttttgct agattagtaa gcgtgcctgc
     2461 atctgcctgt cttgcttttt ccaacgccag agcaatcacc ttcctttggc gctctgtacg
     2521 ctgaaaatcc gtatccataa gccgcagacg gctgtatgct accgcctgaa ctccgtctaa
     2581 atgattggct ccggcctgtt taagctgcgt cgatccaata cccgtggaat tgacggtttc
     2641 tgtaataaat ccattgatat atttgaactc tgccggagta atttccaaat caataccgcc
     2701 taaaacgtta atcgcttcag ccactgcctt ccagttaaag gcagcataat catctatttt
     2761 tagatccagg ttttcttcca gtgcatctac ggcctgtttt tgcccgcctt taaaataagc
     2821 ttcgttgatt ttatggtacg ttcccttgga gtcaattttt aaatatgtat ctctgaatac
     2881 actggctaac cgtatctcac ctgtttctct gtccagtgtg cagaccatct gtacatctgc
     2941 cagcgctttt ttcgtattac cgtcacggct gtctacacca aatacagcca ctgtccagat
     3001 ccctgtagga cgatgaaggt atagaaagct tccatatgcc gtaaccgcta aaagaaggaa
     3061 tatcaaaagc atcggccatt ttttctttct tttacgacgt ctttctctga cagaactacc
     3121 tttcggattg cggcgggatt tctctcctgt cctaaactta tttcgggaac ggctatagga
     3181 ttctctgtgg tttttggaaa aacctcccgc tcttttcgcg gatcgtccct gggatgtacc
     3241 tcttttggaa gactcatctg aaaacggtat atccagtccg tcttcacagc catactcgtc
     3301 ttcaaaataa gcgtcatcta tggaatcaaa ggattcccgt tcccgaaaac tgcggccgtc
     3361 atgggaagag gccctggagc gggaaggcac gtttccgtaa gagcccgccc tcctgcgcct
     3421 ggactgcatt cgtaaaagtt catcctcatt atcatggtaa ttcatggatt ttcacctctg
     3481 tttcattttc tttcataaca gctattctac cacaggaaaa cagataaata cagctgtaaa
     3541 gtcttacgga atcattaaat ctgattcatt gtccactgat ggtaaaattt gtaaatataa
     3601 aaaatatccc ctttcagaca gacagatgaa ataccaaaaa actgctgcca gaaaggggca
     3661 tatcaaaaac ttcataaatt atcgtgattg aaattacttc tgatccttcc attgctgttt
     3721 cagctttgca aaggccataa tagcctctac agcaccatca ggacctaccg aactgctgtt
     3781 taaacacaga tcatagcttc tgggatcacc ccagcgcttg ttggaataat agttataata
     3841 gctggatctc ttcttatctg tctttacaat aacctctttt gctttggaat catccaggtt
     3901 atacagcatc ttaatccgct ccagcttatc tttttcatcg cccgcgataa atacgttcaa
     3961 aacattggga taatcttcca gggcataatc tgcgcatcgt ccaatgatca cacaagactg
     4021 ttcatctgcc agatgcttga tcgcatcaaa ctgcgcaaga aagattttgt gattaatagg
     4081 catatccgag tatcctgcgg ctgtatagcc catagaataa gaatccatca ccaaagaata
     4141 taaaaagctg ctggtaggac gttcatcgtg cttttcaaat aattcctgac agaggccgct
     4201 atgctttgct gcctttgaaa gaagctcttt gtcatagcat ttcactccca tcttttccgc
     4261 cagcttttct ccgatcaatt ttccgccgct gccgcactgt ctgccgatcg taataacaag
     4321 atttcctgac attctgcata cccctttctc tggcctcaca gcaggaacca agctgttccc
     4381 tgctctctct gccatatttg tattataact caaaaattca gaaaagtata tagaaatata
     4441 cagaaaattc tattttaaaa gcacattatt agacaagagt gataaaatcc actaattgtg
     4501 tctaattaat tcagagtctt aagcagattt tcaaagtaat ctggaagggg ggcggaaaat
     4561 tccatataaa gacctgttct gggatgaatg aatccaagga cacctgcatg gagtgtctga
     4621 cctttaagac cggaaatcgg acatttagcc gggccataaa cggcgtctcc caaaagagga
     4681 tgatgaatgc tggccatatg aacacgaatc tggtgggtac gtcctgtttc cagtctgcat
     4741 tctacgtgag taaactgcct gaaacgtttt aatacacgat agtgggtcac tgctaatttt
     4801 cccttgtcat agttgatgct catctttttc cgttccaccg gatggcggcc aatgggagcg
     4861 tcaatcgttc cctcctcttc ttttatgacg ccatgaacga tcgcctgata gcgcctggta
     4921 atagagtgtt cttttaactg agccgcaatc gaattatgtg ccatatcgtt tttacatacg
     4981 atcaatacgc cggttgtatc catatcaatc ctgtgtacga tccccggacg catgactcca
     5041 ttgataccgg aaagctgccc gcggcaatga tccatcagtc cattgaccaa tgtatgggaa
     5101 taatggcctg cggcggggtg gacaaccatt ccctttggct tattgatcag gatcacatcc
     5161 tgatcttcat acagaatgtc cagttccata ttctccggct ctatttctgg ttccaccgct
     5221 tctggaatat ccatggaaag aatatcctca tcttctacca catagcttgc ttttaccggc
     5281 tttccgttta ccaggatccg tccgtccttt aaaagcttct ggatgtagga gcggctcagc
     5341 tgtccggcag cttcggccag atatttatca atacgcagac cgccttcctg gtcgcctaca
     5401 cagatttcct gtttcactgc gttttctcct ttccatgagc ctttccgggc tggaagcagt
     5461 ccaaatcttc atcatcataa taaaaaagca ctaaaagcac cagtcctgct gccgctatgg
     5521 taacgtagca gtctgccaca ttaaatacgg gaaaatcaat caggcgaaag taaagaaaat
     5581 caaccacata attctgggtc atccggtcga tcatattgcc gactgcccct gcggctacca
     5641 gcatcaggca tactgctaac ggacgatatt tcggtgtttc cggcatccgc cacatcatat
     5701 acaggattgc tgccaatacg gctattccga tcaggaagaa aaaaaactga cggttctgca
     5761 gcattccaaa ggcggcacct ctgttttcaa gataggaaaa ttcaaatacg ccttttatta
     5821 cggctaccgg ctcctggttc ttaagaaacc gaaccgccag cccttttgtc cactgatcca
     5881 gccccacaag aatcaaaaaa ccgattaaga agcttaccag aagggatcgt ttttgtctgc
     5941 tcatcattgt tactccttca tatcaatcgt atttctgccc cggtcaaagg cataatcttt
     6001 tttacaggga aacagcctca aaaccggcac ggatttcatc ctctgaaagg gaatgatcca
     6061 cataagcatc tccgattcca tttaaaagga caaatttcac ccttcctgct tccatcttct
     6121 tatcactctt tgtagcatca aatacttcct tccagatcag gccatctaca gtagttggaa
     6181 gttcaaagga cgccatcgta tgtttaatat catctgcttc cgtttcactc aaagtaccct
     6241 ttaaacagct gatacgggct gctgccacac aacccaggcc gacacagtgg ccgtgaagca
     6301 tagtaaaatt ctttaacttt tccacagcgt ggcccaatgt gtggccaaag tttaaatatg
     6361 cccgttctcc ttgctctgta gggtcttttt ccaccactgc tttttttatc tcattgctcc
     6421 gaaagaccat ctgccggcat gtcgtaagat cacgggccat caccgcatca cgatgttcct
     6481 ttaaccactc atagtatgcc ttatcctgaa tcagtccatg ctttatcacc tctcccattc
     6541 cggaagaaaa ctgttccgca ggaagcgtca aaagtgtttc cagattggta tataccagtc
     6601 tgggcatatg aaaggcaccc accatatttt tgtaatccgc aaagtccacc cccgtttttc
     6661 ctccgatgct ggaatccacc tgagacagca gtgtggtggg gatctggatg aagtcgattc
     6721 ctctcagata agtggcagca gcaaacccgc agagatctcc tgtcacgcct cctcccagag
     6781 ctgccagcat atcttttcgg tcaaaatgct ctaaaatcag atgttcataa agcatccgaa
     6841 ccgtatccag gtttttatgt tcttcacctg ctggaaaaat aaaaacagaa accatgctgc
     6901 agcattggct taggatttct tttaccttgt ccagataaag gaatgccaca ttagaatctg
     6961 taacaataca tattttgcgc ttttccagat gaaatgccgc cagttccccc ttcagtcctt
     7021 caaaggatgt ttccatacaa atatcataaa tgggatctcc atccctgcaa accgtcaaac
     7081 gttccttcat acgtcaagtt cctcctgatc tgtctgaatt ttgtgtggta tgaacagaaa
     7141 aacgggggct gctacaggaa ataaaattct cccagtggca gcagcctccg caaatcctgt
     7201 ccctatgtag tttaaatgtg aatggtcata cctgccatct ccataccgga cacatcaccg
     7261 gctgccagca tgtcctgaaa atcaccggac agctccacag aattaggggt tacaatgaaa
     7321 atatagttgg aaattctctg aagattgcca tccatggaat aagcagctcc ggatacgaaa
     7381 tcaatgatcc tctgtgctac ctccatgtgg attccttcca tattaagaac cactgctttt
     7441 ccagataaaa ggtaattaca gatatccttg gaactgtcca tagaggtagg ctttaccatc
     7501 gtaacctcca taccggaacg gcgcatggga accacctttg gcgtaggacg tcccagaaaa
     7561 cgggatttgt cctgaggttc atcgtaatac tcttcatcat ccgcagtatt cttccttgag
     7621 aaaaagctct ttttaggcgg ctcatcttca aagtcatcgt ccaggtagta atcctcaccc
     7681 tcctcttcag gatttaatcg catggtatcc atcaacttac ctaacagact cattttactc
     7741 tctccatttc ctctctatca acgctgcctt acacagcgcc gtaatgccgc tcaccaaaaa
     7801 taccggtgcc gacccttacc atggtagcgc cttcctcaac agcgacctca taatctccgg
     7861 tcatacccat agaaagcaca tccatagtaa cattatcaat gtttttccta cctatgtcaa
     7921 cagcaaattc atataatttt ttaaaaattt cccgattttc ctctggattc tcaacaaatg
     7981 gcgcaattgt catcaatccg cggatatgca catgagggaa ggtgctgatc tcccgtatgg
     8041 ccgcctcggc atcttccata aaaaagccat atttgctctc ttcttttgcc acattgattt
     8101 ccaaaagaat atccacttct atattctttt ttgcagcctc ctcctcaatc tgtctggcaa
     8161 ggcgcaggga atccacagaa tggatcagga cggcctttcc caaaacctga tgaaccttgt
     8221 tgcgctgcag atgtccgatc atatgccagc gcacatcatc aggaaggctg ccatattttg
     8281 cctggatctc ctgaacctta ttttcaccaa aatccctggc tcctccctca taagcttcct
     8341 caagcatggg aacaggtttt gtcttgctta ccgcaataag cgttacctcg gacgcatctc
     8401 ttcccgctct ttggcaggcc tcctcgatac gccttcttac tatttccaga ttgtctctta
     8461 tctctgacat cgtttcatcc tcctctgtca aatcctgatt taaaacagcg atcctattga
     8521 tacaggatct ggccttccgt aaccagagag gcatccagca caatatggtc atatacagac
     8581 aaaccgtagc ttgctccttt ttttatggta tagtattcgc tgttggaatc cagaatttca
     8641 atttctttaa atacggtata tcccttgtta atgttaaaaa caccctttaa agccctggta
     8701 cttcccacct ggaaccgctc ctgggaatca ggctttacaa tatagtcgcc ggcttttaag
     8761 gggccgtcct cctgaacagc aatataataa aattcttcat catcaaaacc gatttctata
     8821 ggggtaaaga cagctgttgt tccatttgca tcatagattt ctttatttac accgctgtct
     8881 gtactgtccc cgccctgagt gacaaaatct cttgggatct gatagaaatt tttctcagtt
     8941 actgcactga cagggatttt aagccccttt gcctgatcct ggcgaatctc aaaattcaca
     9001 aaacggtctg tgataaactg tgccatgtat ttattaaaat ccagcttacc gaaaacagag
     9061 ccgtcctttc ccgtaaaaat agaaaaagaa gcggtggttt caatactgtg atccttaaat
     9121 tccacctcaa gcgaggactt gtccccatag agggccctgt cctcctctgt aagagggaat
     9181 acaaccgacc agttttcaga agttgcaatc ttataggcag gtgttccgct ctcgatcaga
     9241 tctcctgatt tatgaaaggt tttcgtatag gcagaccggt caaaagatgc cgtctccact
     9301 tcctcaggct taatactttc ataggaatca aagccatagg ataccacacc tgactgaggc
     9361 gataccacct gttcaaagac aatgccggat tccctggcaa tctgatccag ctgatccaga
     9421 gcactgaagc tggaatactc catcactgcc gcttccaggg tataacgggc atcataaatc
     9481 gccccgaaat cctcgtccga acggctggta acaaaggagg agagctgttt ttttaaatca
     9541 gccaggtttt catcactgag cgtctgattc tcaaccccat gctcctttaa aagggaatcc
     9601 aggcggccgg tttcgtccag agaataaacc ctggaaccta cggaggcacg tttgccttcc
     9661 cgtaggtaat agttgatata cccgctgtta tccgcattat agacttgttc ttctctcagg
     9721 atcagtcctg tgtagggacg gtcatttaca ataccgcctt ctgccacctc ataaaactgg
     9781 attttttcat gtcccatata gataaaaata ttgatcagaa gatacagaaa gataacaccg
     9841 aatatcagct tgccgatatc attgcgtggc cgccggtaag gaatgatctt tctgtttttt
     9901 ctgtgctttt tactggccat gaataacggc tctcctttta aagtcttgtt tcgtaaaaaa
     9961 gctgccctcc gcaccaggcg tacttcaaaa cctcgtgcgg caggcagcct aagttctgtc
    10021 tgctatttta tcacagaata tgtatcatta caatgaaata atcacatcac cctttgcatt
    10081 ctccggaagc tcatccgcgt ccatggaaat gcttaatacg aaagtaacat gatatttgtc
    10141 actgagtttt ttcaatgtct cgtaggtctc ggaaatgtcc gctccctcta agcacgccag
    10201 ctttaagaag ctgtcaagat acatagtctc aagatcatga tcctgagaaa gaatgccaca
    10261 gataaatcct aaaaatcctt cgctgtgtgt gatcggaaat tcgttcacat tgataagacg
    10321 gatccggttg ttcagctcat acatatgctg agcgctctta tccagatata caacggtgcc
    10381 gctggctccc ttgattgctg cattggccat gtctaacaga tgttttgtct tgcctttacc
    10441 tttttttcct gcaataatct gaaccatacc tatctcctcc ttgagttata tatgttgtat
    10501 aaaaaatcaa ttaagattac cttgattata gtacaaattc atgcggaacg caatcactat
    10561 ttgacgattt tagaaattat atttgtcata ttatcataaa gatgggcgcg gctgtccgat
    10621 ttcagcacaa cagatataaa atttccttcc gaggcatcat cctttgatgc aataattaag
    10681 caatagccgg ctgcttttgt ggtacccgtc tttccgccta acacggtaac tccctccggc
    10741 gtatcccgtt cccctgtgat aaactggttg ctgcatttcc aggttctgga tacactctct
    10801 ccggccccat taatgtaatc tgccgtgtac gccttcgcct caataatctc ccgaaatttg
    10861 gggtatttga aggcctcgct gaatatcaga tagagatcat agactgtggt ataatgatcc
    10921 tcatcatgaa gtccgtgcgg atttacaaaa tggctgtcca ctgcgcccag acgtttcgct
    10981 tcctcattca tccggtctgc aaaggcctct atactgccat ccatatgaac cgcaatggct
    11041 acacccgcat catttccaga cggaagcata agaccataga gaagctgctc cagcgtaagg
    11101 gtatcacccg gcttaatgcc gcagagtgtg gcgcctgact ctgtaattac cgcttcatcc
    11161 gtaaccttta cctgatcctc cagattgccg tatttgatcg ccaccagagc agtcattacc
    11221 tttgtaatac tggccggata catggtctca aaggcatcct tgctgtaaag cgtctgatga
    11281 ccgttcagat caaaaagcgc tcccgcctca gaggtgagaa aatccgggtc aaagacactc
    11341 tcatcggata caacgcagag atctgaggcg aaaccttcca tgacttcttc ctctgaacct
    11401 gccaaggcaa tcgccggaag gcgctttttc agagaatacg catcagaaaa gccgccaaag
    11461 cagcctccaa gacacaaaga agcagccaat atgagggcac tccaagctct gacatacccc
    11521 cgcttcatta tctgtcaacc tctctccatt ccatatcacc tctgtcaaga gccttgatca
    11581 aaagctccgc agtcgccaga ttggtagcca gcggaatgtt gtaggtgtcg cagagacgga
    11641 ctacatcatt tacgtccggc tcatgtgact tgggcatcag gggatctctc aaaaaaataa
    11701 ccagatccat atcgttatgc tcaatctgag ccgccatctg ctgaatcccg cccagatgac
    11761 cagccaaatg tttgtggata ttaagatttg caacctcttc tatgagtctt ccggttgttc
    11821 cggtggcata cagagaatgc ttgcttaaaa tccctctgta tgcgatgcag aaattctgca
    11881 tcagtttttt ctttgcatca tgtgcaatta agccaattgt catattcaga cccctcctta
    11941 ggaattagta gtgtcgtggt acccgctgca gtcctacatt gagcacaagc cccataccaa
    12001 taaaaatact gatcagcgaa ctgcttccaa aactgataaa cggaagcgga agtccggtat
    12061 tgggaaaaat acctgtagca actgcgatat tggcaaaact ctgaaatgca atgagcgtag
    12121 ccataccggt acataacagc ctgccggcca tatcttttgc cctggctgcc atcataagac
    12181 attcatatac aatcagtaaa aacagcaaaa tcacgatggc gcagccgata aaaccaagct
    12241 cctcaccgat aacggcaaag ataaaatcag tctgctcctc cgaaagaaaa ttaccgtttt
    12301 ttacagatgc aatcgtagtg ttgtaaagcc cctttccatg cagctgaccg gaaccgatcg
    12361 cgataatgga gttattctgc tgataacgtg cctctccata ctgctccggg taaaaccagg
    12421 ctaagatacg tcccgcctgg tattctttaa taaacgggat cataccatga agaagaaggt
    12481 acacgcctgt cgcaatcata gggatgacaa ctgccagtgt ccccacgatc cagcggtagc
    12541 tgatgccgct ggcgaacaca atccccagca cgatcaccac agtaacgaga caggtagaaa
    12601 ggtttggctg ttcaaatata agcatggccg gaacggcaaa aagcgctgcc gccagtacca
    12661 cggtagaaac cttgtctacc ttctcctgat atttcataaa ataccaggag aaggtgatga
    12721 taaggccaat ctttacaaat tccgagggct ggagctgtcc caaaacagga acctcgatcc
    12781 agcgttttgc attattgacg gatctgcccc aaaacagcac ggccagaagg cttccgatgc
    12841 agatcagata aatgaccgtg ctgaaattca gtatcttatg gtaatctacc agtgaaagac
    12901 aaacagcaat agccatgcca gccagtatgc caaacatctg ttttgtcact gcttccgaat
    12961 ccatattggt ggcactgcgc acgaccagta tgccaatgac gcttaaagct gtcatatagg
    13021 caagaagccg gaagctgtaa tttctgaaat cataatcaaa aagcatatca gtaaagcccc
    13081 ttattggaaa tggagcgtat gggtatatct gcctggagca ccggcagccc ccggcaggag
    13141 gcggaaccgt ctgtgtccat caaaaggcgg acattgtcct tttctatctc catgtatttg
    13201 gaaatgacac ggatcatgtc atttttgatc agccttatga gttccggcga acagcctgcc
    13261 ttatctgcca caaggagaag cttcagccgg aatttggccg tatctttgga acgtctggtc
    13321 ttcacgttgt ttcacccctc ctatgcccgc ttcagcaccc gggccagcct tacaagaaag
    13381 cctctggcct cgccggggga ttcgatgggg atattctgcc ctaagagcct gcggcataca
    13441 ttcaggtaag cctgtccggc ggcggaccct attcctacca agggttctcc ctgattggtg
    13501 gagactacga tgttttcatc atccggaata gcgccaagga tatccacggc taaaatatcc
    13561 cgtacatctt ctaaagacat catgtctccc cggcgtacca tatccgcccg gatccggttg
    13621 actaccagcc cgatttcagt aatgcccgcg tgttccagca ggccgatgat ccggtctgca
    13681 tcgcgaatgg ctgaaacttc gggagtggtc accaccagag cccgtcccgc tcctgcaatg
    13741 gcattgtaaa agccctgctc aatccctgcg ggacagtcca gaaggatata atcaaactcc
    13801 tccttcaggt cttctgtcag cttaaccatc tgctccggat ttaccgagga tttgtccctt
    13861 gtctgggccg cgggcagaag gtaaaggctt ggatatcttt tgtctcttat cagggcctgc
    13921 ttcatacggc agtttccttc taccacgtct acaagattgt agacaatgcg gttttcaaga
    13981 cccatcacca cgtccaggtt gcgcagaccg atatcggtat caaccaatac cactctcttt
    14041 cccagaacgg ccagtcctgt tcccacattg gcagaagtag tcgtcttgcc taccccgcct
    14101 tttccagaag taatcacaat gacttcactc atatagacct cctgaaaaaa tgaactttac
    14161 ccatccattg tacattaaat tttgtgaatt taccagtatt tttttccatt caagaaaaat
    14221 ttaatacagt tcggcaaaat tttacatata tcagcccata tccccaaaaa tggatcgtcc
    14281 ttttacaggc cggaacgcta tttcaccgtc cctgatacag gcgatcatgg ctcctcggcc
    14341 gaaccggcgg tgtttttcat ttgttggaat cggacagttt ccgatacgaa gctgaaaagg
    14401 catcatggaa aaggccataa ctactgcctg agcatttccg gaaacacctg ccgtcacaga
    14461 accctttagc tcgcccaaga cgatcacatt tccctttgcc gtgatcctgg ccccatgctc
    14521 cacatctccg atcactataa tgctggactc tgattccagg gtatctcccc tgcggagtgt
    14581 tccacggtaa aactgtccgg tctgggcact cagctccata agcttttcat tcagagcttt
    14641 ttcgcagcgt tcgatccgct gggcatccgt atccagaaga cagagcacat caatctgtga
    14701 attttccgta atagtattta ccacagccat ctcctcttca ggtgtaagcc tgcgcccttc
    14761 cagtgtaaga gtcatctgaa ctgcccccca gaaacgggcg ctgtctttaa atttttttgc
    14821 aatagcggca agaagctcct caaaggggag atttggatcc agcagcacag tcatgcctgc
    14881 ccggctgctt ttaatgacga tcccgttttg catatgatca ccttcctcct gtatgatatc
    14941 attcttcaac ggagatgttt gatgcgctta aggctcctga gcccacaatt tcttcaaagc
    15001 tcgtatagcc gtaatagtat tcatagatct gttttccaag aggagccgca ttggttcccg
    15061 catagccata tgggatattg accgttaccg cgatctccgg attggaataa ggagcaaagg
    15121 aaacgaaaaa tgcatggttg gaccggctcc gatcctcctg tgccgtacct gtctttcctg
    15181 ccacttctac atgggagcct gcgaatacgc tttttgcaga tccctctgta accacccggc
    15241 ggataccgga ctggacagca tcccaggtag aagtctttgc ttcaatctgt gaactgattt
    15301 caggggtata atcttcaatc agatttccgt ctgaatccgt aagcttgtcc aggaggctca
    15361 gttcaaagac cgtaccgcga tttgccacag cggaaatgta gcgggaaagc tgcacgttac
    15421 tgtaggagtt ggttccctgt cccatagctg aacgctccgg atcctctgtg gtcagatgag
    15481 gctctgtttc aggaagttca atacctgatg tgtgatccag accgaacatg gatgcatatt
    15541 tgcggattgc ctcaagtccc ttttccgaag aataggttcc atcctctgta aggcttaaac
    15601 ggtgtccaag ctctgcaaag accacattac aggaattttg gattccggtc acaatatcct
    15661 ctactccgtg acgtcccggg aatacccagc agttaatagg cgggctggac agatcatagg
    15721 agcctgtaca ctcaatggta tcggtaagag atatcacgcc ctcctccaga gctgccacgg
    15781 cagtaatcgg cttaaaagtt gagcccggcg ccttgatggc ctgagttgca ttattataaa
    15841 gaggattgga catatcgttc tggagtttcg caaaatacgc cgcatccaca ctgcctgaca
    15901 tcttgttatt atcatagctt gggtaagaaa cgagggctaa cacctctccg gtgcgcacat
    15961 cagtcacaac cgctccggct gtacagggat ctaaggccag ctgcgctgga gtcagctcca
    16021 gattggaaat tttcttttta ataaagttgt aggcatatac actgtctcct ccggctaaag
    16081 ccgcctgtgc tccgggatcc ggctccagta cgccctggtc aaagagggcc agacagagtt
    16141 cgctgcctct gatcacctcg ttattgatca gatagcggaa catacgctta gtaaatttcc
    16201 catcttcctc cagatgaaca caaatgtagt caaccagctg tgcaaagctg tcatccgcat
    16261 tggaatactt gttctccgag tccagctttg tagtgtcgat ccagttgccg gatataccgc
    16321 tgtagatata atccctgagg ctgatggttc cttctttcca ggaaagatac tcctctgaac
    16381 tggtatcaat cttatcacgc ttgatgattc ctgtttcttc ggaagccaga aaactgtaga
    16441 tataattcat atagcttttc atatcctccg gaagatcatt catcgctctg gcatgaggac
    16501 ttaaaagctc attctttata tctgtcaaaa tctgtgtttt ggatgaggta taggtgctgt
    16561 aaatcttatt ttccacagaa gaggcatccg gctcagccat agcctccaga gaaagcacat
    16621 tgttattgat caactgataa tacgcattct taacaggaat cggcttttta gatccgtcct
    16681 ttactgtggc aggatctaca tcctcgttta caaggaattt gatgataatt cctgccagct
    16741 gctgctccat cagcttatag gtggcaatct gaagatcccg gtcgatggtc agatacacat
    16801 catttccagc cttggcgtcc tgctgctcaa tgacctccct tggccgtccc atattatcca
    16861 catacatttt ggtatagccc ttttcgccct gaagatccag ttccatatac tgctcaatgc
    16921 cgattcggcc aaccacatca ttgaggttgt atttttcatc ctcctcggtg agatttttac
    16981 cctttttatt ttcttcctct ctccactgct cattcagtcg gtctacctgt tcttcctgaa
    17041 cctttcccgt atatccgata atgggggaga aatacaggct gtcattgtat acacggatat
    17101 agctttcttc tacagaaaca ccctttagat cagccgcatt ttcatggatc tctgtcatgg
    17161 tctcccggct tacattggtg gctacggtag tgctctcgta tttctggtac tccgtaagct
    17221 tcatggtata gatgatattg atcatatcga gagccgtctg atccgtaagc tctaagggat
    17281 tgccttcttc atccttcata tcgtttaagt tatagccgtc acggccggag ctttttttca
    17341 gctcaaaggc ttctcttgct gtaatgtccg aaggataacg gccgctggca tcatccaact
    17401 tttcggagct gattccataa aaattaagca gaaaacgctt gtgagaagcc ttagaggtgg
    17461 tggtgaaata catctctccc tgctcgttta tggcaatctc cagctttcct tctacctttt
    17521 ctcctcttcg ctccaaaatt gtcaccaggc ggtagatcat ggcatttcga tctgcaggcc
    17581 ttggataatc gcctagatcc tggatcgtaa ccgaataagc cagttcatta taggcaagaa
    17641 gctttccatt gcggtcatag atattgcctc tcgttcccgg cgcagtaacc attttttccg
    17701 ttcgctgcat ataggactgc tgatattcat ctccgtggat aatctgcagg ttaaacagct
    17761 ttccaatgag ccctgcgtac atcagagtga aaatcactgc caaagcaaat agacgggagg
    17821 aaaaaagctt ttttaaaaat tcctttacaa cttctttcag ttcatcaaac aatggtggta
    17881 tctctccttt ttcctatgtc ctctacccgc cggctggcag acaggaacag acgatatatg
    17941 agaagggtgg taacggctgt aaaaataatc tccggaagca tgatcttcgt aaaataatag
    18001 ggaagatcta atcgtccgcg gatcaggaaa cggaatatat aaatatacat gcagtaccac
    18061 agaccgtttg ccacactcag gattaaaggc agggtaatgt agtcttcata ataatatcgg
    18121 gtaaagattc cgttaaaata accgatataa atatagatca gggtataaaa accaaatgag
    18181 ccgctgtaga atagatccag caaaagcccc gaaatcacgc cgtagaacat ccctgcgttt
    18241 tttccatgga taaaaccaaa ggaaaaggtt aagatcagca gcagattggg cgttgccgcc
    18301 agaaagggaa gcagggggaa tacacagttc tggacggtaa atgcaaggat aataaagccg
    18361 atgttgatcg caatctgttt cacctttgcg ctggtcactg tttctccacc tcttctccat
    18421 ctttcagatc ggtcaggatc agaacctcct ggaggttatc gaaattggct gcgggaatca
    18481 gataaccgga ctgggtcaga tgatccggat caatggtaac gtccaccgca tatccgatca
    18541 gaatgccagg aagaaacttc gtactgatat tggaagtgat gatctgatcc ccatccttta
    18601 acacagcatc cttggaaaaa tcggtcagct ttaaacgtcc ctgctcatag agggttaaat
    18661 ctcccgccac aatacagtta taggaggaat ccacagacat ggcgcctaca cggctggaat
    18721 cgtcaataat tgaacgcacg gttgcataat tggctcctac gtctgtaacg attcctacaa
    18781 ggcctccgtc tgccactaca ttcatatcca cagccactcc atcggaagat cccttattga
    18841 tccgaaaaac ctgaaaccat ttttcagagt cccttgcaat cacccgggcg ccgattttat
    18901 tatactgcat gtattcctga tccagctcat atagctggcg cagtcggttt aactcgaact
    18961 gctgggcctg gagccggtta ttttcctcgg tcagccgatc cacctggatc tttaaacggt
    19021 cattttcgtc cagagccgtc tttaaacgtg aaatatccgt aagctcattg taaagtcctg
    19081 ttcccacacg gtttacaccg gtctgaatag gaatcagaaa ataccccaca ccggttctta
    19141 gaggctctaa gataccgtcc tttaaggagg taatgccgat caacaggaca caaagcaggg
    19201 taagccctga gaggatatat ttgctgcgtt ttgattccat ggatattcat tcctctttca
    19261 tgtgttatat gattatataa gtttttcccg gcgaaaactt aaataccgcc ggtctccgat
    19321 gatcaaatgg tcgatcaggg taatccctac gacctctcct gcttcccgga tccggcaggt
    19381 taaaagccga tccgagcggc tgggctcagg gtccccgctg ggatggttgt ggactaaaac
    19441 catacctaca gcaagacaac gaagcccttc aatgaggatt tccctgggag taaccacaga
    19501 agcgtttacc gttcctttgg aaacaaggaa atcacggatc agatgctgtt tggtattcag
    19561 aaacatgaca tggatttcct cctgttccaa atgacgcata tcttccatgt aataatccgc
    19621 aatctgatcc ggctgtgtaa aagccacagg ctctgcgctt accttctgct tccaaatcct
    19681 cctagaaagc tctccgatgc acaaaagctg gattcctttt acaggaccta ctccacggat
    19741 ggactgcagc tgttccagag agcagtgcag aaggcctgta agtccatctc ccggtgcatt
    19801 ttgtgatagg atggcgtctg ccagttcaag agacgtgcgt tcactgctgc cggttcgtat
    19861 gatgactgct aacagctcgc tgtccgtaag cgcctttgga ccgtaagcca tgcatttttc
    19921 atagggacgc tgctctttgg gcagatcctt cattgtaatt ctttccattt atcctcctga
    19981 ctacgcacct ctccaagtac attttcagga tgagctttta aatcatttta taaataaaca
    20041 gagccactgc cactccaata atgcttgcca tggtaatctt gatggtaagt ccgaaagtaa
    20101 tgactaaaag gccaaaatca atgaccaaag gagtatccag cccaaaggac tggccataat
    20161 tcatccattt cagccaggaa ataccatctg ccagctgtcc gataaatcct cccagtacaa
    20221 ttccggctaa gagcataata agaaggatcc agaaattttt acgtcccata aacaaactct
    20281 cctaaggaag ccccgaagga cggggctgtt tatcatatta ccataagttt ttttaggtgt
    20341 catctgaaaa aattcttaag aaacaaggct tttaggggac acaactccat catccgccag
    20401 ctgctggagg gattttaaaa ttccaagctt ggcatgatac caggtaagcc ctccctggaa
    20461 atatacggca tagggcggct taatagggcc gtcggcactg agttcaatag aggaaccctg
    20521 tacaaaggct cccgcagcca tgatgaccgg actgtcatag ccgggcatat cccagggttc
    20581 gggggtgaca aaactgtcta ccggagcagc tgcctggatt cccttacaga aggagatcac
    20641 tccctccgga gtaccgaagg taatggcctg gatgatatca tgacgtgatt cggagccgtt
    20701 tggcactacg ccgtagccca gacgctcgta gatattggcg gcaaaaatcg cgccctttaa
    20761 ggctcctgct acaacggtag gtgaaaggaa cagaccctga tagagctgct gattcagtcc
    20821 taagctggcg cccacctctt ttccaaggcc gggggcactt aatcggtagg aagcacggtc
    20881 tacacagtcc tttcttccgg caatataacc tccaataggg gccagaccgc cgcccggatt
    20941 cttgatcagg gagccaacga tcatatcggc tcctacatcc gtaggctcga tggtttccac
    21001 aaactctcca tagcagttat ctaccatgca gatcacctca ggcttgatct tttttataaa
    21061 ggcgatcagc tccccgatct gctttacaga aaaggtagga cggggatcat agcccttgga
    21121 acgctgaatg gttacaagct ttgtcttttc attgacagcc tttgcaatat tttcgtagtc
    21181 aaaggttccg tcgggaagaa gatccacctg gcggtaggaa acgccgtatt cctttaagga
    21241 acccacggaa tccctgatac cgatcacttc ctccagggta tcatagggct ttcccacagg
    21301 ggaaaggagc tcatctccgg gacgcaggtt tccggaaaga gccacatgca gggcgtgggt
    21361 tccgctcata agctgtggac gaaccagggc gctttctgtg tggaatacgt gcgcatatac
    21421 ctcctctaag gtatcacgtc ccaggtcatt gtagccatag cccgtggtag ctgcaaaatg
    21481 gatatcgctg acgcggcatt cctgcatggc atgaattacc tttaactgat taaattctgc
    21541 ccgttcatca atctcctgaa agcgggtttt taaatgttcc tctatctctg cagcaaaatc
    21601 cagtaccctt ttgctgattc ccagtgtttc atacatatcg gttaatacat tctgttccat
    21661 tggtctgact ccattctttc tttttgtgac ctttttcaag gttcggtctg tcattgtagc
    21721 acgattccct cgtccatgca atccttcaga atctgtttta acgcgtttga cgtatcgtct
    21781 ggcgcgcctg aaagttcaat ccagcgcacc tggcgctctc tgcgaaacca ggtcagctgg
    21841 cgcttggcaa aatgccttgt atcacgtttc agtatataga ctgcttcctc caggctgcag
    21901 cgcccttcca ggtagtccag gatttcctta tagcctaatc cctgcatgga ggtctgcccc
    21961 cgatggcagc ccatttcttt taagcgctgt acctcatcga tcagcccctg ctcaagcatc
    22021 agatccaccc tgcggtcaat cctgtcatac agtctggtcc tgtctgtatt cagcacataa
    22081 tagaggaaat gataagggga ttccttctgg tgcatggctt cgttatgatg tgagattttt
    22141 tctcctgttt gtctgaaata ttcgatggcc cggattaccc gctttatatt gtttggatgg
    22201 atctgggcgg ctgcctcagg atc
//
LOCUS       DBMDUH010000027        22158 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_1511_length_22158_cov_7.169833, whole genome shotgun sequence.
ACCESSION   DBMDUH010000027 DBMDUH010000000
VERSION     DBMDUH010000027.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 22158)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 22158)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22158
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1511_length_22158_cov_7.169833"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(8..2833)
                     /gene="uvrA"
                     /locus_tag="ACZPCG_03955"
     CDS             complement(8..2833)
                     /gene="uvrA"
                     /locus_tag="ACZPCG_03955"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002596050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR1284262.1"
                     /translation="MAKQYIKIRGASEHNLKHVSVDIPRDELVVLTGLSGSGKSSLAF
                     DTIYAEGQRRYMESLSSYARQFLGQMEKPDVESIEGLSPAISIDQKSTNRNPRSTVGT
                     VTEIYDYMRLLYARVGIPHCPKCGREIHKQTIDQMVDQIMAMPERTRLQLLAPVVRGR
                     KGEHVKVLEQARKSGYMRVRIDGNLYELSEEIHLEKNIKHNIEIVVDRLVVKEGIENR
                     LTDSIETVMALSGGLMTVDVIGGEPVNFSQSFSCPDCNISIDEVEPRSFSFNNPFGAC
                     PECFGLGYKMEFDENLMIPDPSLSINQGAIVVLGWQSCTDKGSFTRAILDALAKEYHF
                     DLDTPFEAYPKEIHDILLYGTGGHEVKVRYKSQRGEGVYDVAFEGLIENVSRRYKETF
                     SEASKAEYETYMRITPCPVCRGQRLKKESLAVTVGDRNIYEATNMSIVKFKEFMDELK
                     LTPMQQTIGESILKEIRARISFLIDVGLDYLSLSRATGTLSGGEAQRIRLATQIGSGL
                     VGVAYILDEPSIGLHQRDNDKLLKTLLHLRDLGNSVLVVEHDEDTMRAADCIVDIGPR
                     AGEHGGQIVAVGTAEEIMANPDSITGAYLSGRIRIPVPEKRRLPAGWITVRKAAENNL
                     KNIDVSFPLGVMTCVTGVSGSGKSSLVNEILYKALAKKLNRARTIPGKHKCIEGTEQL
                     DKVIAIDQSPIGRTPRSNPATYTGVFDLIRDLFASTSDAKARGYSKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVPCEVCGGKRYNRETLEVKYKGKNIYDVLNMTVEDALT
                     FFENVPSITRKIQTLYDVGLGYIRLGQPSTELSGGEAQRIKLATELSKRGTGRTIYIL
                     DEPTTGLHFADVHKLIEILRRLSDGGNTVVVIEHNLDVIKTADYIIDIGPEGGDKGGT
                     VIAKGTPEEVSESPVSYTGRYIKKYLM"
     gene            complement(2927..3211)
                     /locus_tag="ACZPCG_03960"
     CDS             complement(2927..3211)
                     /locus_tag="ACZPCG_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007715688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6809 family protein"
                     /protein_id="HIR1284263.1"
                     /translation="MSSILEQLYAGDLTPAENVIQGNEEYDRLCRQSVQEIEEFSKKL
                     DDESRKSFDALLEHYLELTYIEKSQTFCDGFRLGAGIMCEVFKGRECLCR"
     gene            complement(3336..5225)
                     /gene="ftsH"
                     /locus_tag="ACZPCG_03965"
     CDS             complement(3336..5225)
                     /gene="ftsH"
                     /locus_tag="ACZPCG_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009295574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIR1284264.1"
                     /translation="MGIKNSDDGNKKPFLYYYIIVLLVVMVLNALVFPSMMERSMEVP
                     FSEFTAQLDAGNIKEAYVSSDNTEIVYSLKPEEGARPQWSAITYKTGNMVTEDELEAK
                     LSAAGVEVYTKQIPHKESPLVNFLMTWIFPILMFVVIGQIMARMMAKRMGGGNAMTFG
                     KSNAKIYGENETGVTFRDVAGEEEAKDALKEIVDFLHNPEKYADIGANLPKGALLVGP
                     PGTGKTLLAKAVAGEAHVPFFSISGSEFVEMFVGMGAAKVRDLFKQANEKAPCIVFID
                     EIDTIGKKRDGGGMSGNDEREQTLNQLLTEMDGFDGKKGVVILAATNRPESLDPALLR
                     PGRFDRRVPVELPDLKGREAILKVHGQKVKMSDDVDYNAIARATAGASGAELANIVNE
                     AALRAVRFGRKAVCQEDLQESVETVIAGYQKKNAVIPPDERRIVAYHEVGHALVAACQ
                     THSAPVQKITIIPRTSGALGYTMQVDQGERYLMSRDEALNKIATFTGGRAAEEVIFHS
                     ITTGASNDIEQATKIARAMVTRYGMTDEFDMVAMETVNNQYLGGDTSLMCSPETAKRI
                     DEKVVQIVREQHEKAIHILKENETKLHEIAAYLLDKETITGEEFMEIFEKQSADGENT
                     LKEEE"
     gene            complement(5409..6158)
                     /locus_tag="ACZPCG_03970"
     CDS             complement(5409..6158)
                     /locus_tag="ACZPCG_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007715693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIR1284265.1"
                     /translation="MSGNKKEEALIHVQGLGKSFGAVDVLKDISVDINKGDVVFVIGP
                     SGSGKSTFLRCLNRLEEPTRGHIFFEGTDITDPKTNIDKHRQKMGMVFQQFNLFPHMD
                     IMKNLTIGPVKLQGISQEEAEKEAMRLLERVGLADRAHAYPSQLSGGQKQRIAIVRAL
                     CMKPDVMLFDEPTSALDPEMVGEVLNVMRDLAEEKMTMVVVTHEMGFAREVATRVLFM
                     DGGYIMEEAAPEEFFTNPKNERLKSFLSKVL"
     gene            complement(6148..6840)
                     /locus_tag="ACZPCG_03975"
     CDS             complement(6148..6840)
                     /locus_tag="ACZPCG_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR1284266.1"
                     /translation="MWENFTNAFYLNFISEDRWRYITDGLQTTLIITCFACLMGIVLG
                     FIVGMIRSTHDKTGKLKFLNVICQIYLTVIRGTPVVVQLLIIYFVVFGSARIDKVIVA
                     IMAFGINSGAYVAEIFRSGIMSIDPGQMEAGRSLGFNYFQTMRYIIMPQAFKVVLPTL
                     CNEFISLLKETSVSGYIAIQDLTKGGDIIRSRTYSAFMPLIAVALVYLTIVLIFTKLI
                     QILERRLRQNER"
     gene            complement(7022..7864)
                     /locus_tag="ACZPCG_03980"
     CDS             complement(7022..7864)
                     /locus_tag="ACZPCG_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR1284267.1"
                     /translation="MKKYVLAFTAALMAASLTACGGSAAETTAAKTEAKAEDTKAEDP
                     KAEETTAAKEETEAAGGVLVMATNAEFPPYEYHDGGEIVGIDVEIAKAIADKMGMELE
                     IEDVAFDSVIPEITSGKADMGLAGMTVTEDRKVSVDFSDTYAKASQMIIVKEDSAIAG
                     PDDLKGVVVGVQLGTTGDIYVSDLEAEGTTVERYNKGFEAVQALSQGKIDAVVIDGEP
                     AKTFVAETEGIKMLDEAFTEEEYAIAVKKGNTELLDKINGALSELESEGTLDEIVAKY
                     ITAE"
     gene            complement(8002..8847)
                     /locus_tag="ACZPCG_03985"
     CDS             complement(8002..8847)
                     /locus_tag="ACZPCG_03985"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006776307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HIR1284268.1"
                     /translation="MELLKGAPVSAAIREWAAEEIKQLQGSVPKLAIIRVGEKPDDMS
                     YERSAVKKMEAFGLRVQIFVFPKDISDEEFKEAFQKINKDPDVTGILLLRPLPRQIKE
                     KEIEAMIDPKKDLDGISPVNIAKVFSGDPSGFAPCTAEAVIEILKANNISIEGKRAAV
                     IGRSMVIGKPLSMLLLKENATVTICHTRTKELPEICRQAEILVAAAGKAGMVEESWVS
                     DGAVVIDVGINVDENGKLCGDVDQASMESRNLLLTPVPGGVGAVTTAVLAKHLIQAAR
                     SNVLQ"
     gene            complement(8884..9513)
                     /locus_tag="ACZPCG_03990"
     CDS             complement(8884..9513)
                     /locus_tag="ACZPCG_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HIR1284269.1"
                     /translation="MTAEAQIKDYLELLSSKAPVPGGGGASALAGALGDSLGQMVVHL
                     TLGKKRYAQAEQDMLNYERELKRLQQEFLMLADKDAEVFAPLAECYRLPSSTEEEKAY
                     KEKVMEERLVQASYVPLEIMEKAAEMLGILKELAEKGSVMAVSDVGVGVQFARTAILG
                     AVMNVYINTRSMKDRKRAEELNREAKRLTKEGTDAADRIYEKIVKQLQG"
     gene            complement(9750..10472)
                     /locus_tag="ACZPCG_03995"
     CDS             complement(9750..10472)
                     /locus_tag="ACZPCG_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIR1284270.1"
                     /translation="MTELAYDLHIHSCLSPCGDDDMTPANIAGMAALKGLDAVALTDH
                     NSCKNCPAFLAAAKEYGIIGIPGMELTTSEEVHAVCLFPELYQAMEFDRFVYSRLIKV
                     KNRPEIFGKQQIYDENDSVYGEESCLLINAVNLSFEGLWEKAASYGGVMFPAHIDKNS
                     NSLIANLGFIPPDSSFQTVELKDMKKLHSLKREHPYLEHCRIISNSDAHYLEHINERG
                     FTLPAAERSAKAVVDTLRRGLF"
     gene            complement(10469..10801)
                     /locus_tag="ACZPCG_04000"
     CDS             complement(10469..10801)
                     /locus_tag="ACZPCG_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009298499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284271.1"
                     /translation="MTVQELIDKNVFQEINIGDDTDRSITKPFCCDLLSIAMGRAPAG
                     CAWVTVMGNLNTLAVAALADAACVILAEGAMLDEAAVKKAALQGITVLRTEEPVFETA
                     LAVKELLK"
     gene            complement(10798..12195)
                     /locus_tag="ACZPCG_04005"
     CDS             complement(10798..12195)
                     /locus_tag="ACZPCG_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HIR1284272.1"
                     /translation="MDKFYHSVRLDPSLCKGCINCIKRCPTEAIRVRGGKASINKKFC
                     IDCGECIRVCPHHAKHATYDKLDTMNQYEYTVALPAPSLYSQFNNLDDSNIVLKALLR
                     MGFDDVFEVSAAAELVSEATRQYISDHEEQLPLISTACPSVVRLIRVRFPSLIPHLLP
                     LNPPVEVAAILAARRAMEKTGLPREKIGIMFISPCPSKVTYVRAPLGTEKSQVDKVLA
                     IKDVYPRLLAAMKSVAGEDYPAIGTSGKIGISWGHSGGEASGLFTENYLAADGIENVI
                     RVLEDMEDQKFTNLKFVELNACNGGCVGGVLTVENPYVAEVKLKRLRKYMPVARNHME
                     DGELDAVKWTTQIQFEPVFNLGNNMMESFLRLNQAERLVKKFPGLDCGSCGAPTCKAL
                     AEDIVRGEASETDCVYYLRENLHRLSEEVAILADDLHSKDKSSQDTLNTLKECIRRIS
                     DEMSSLDSREEGEEE"
     gene            complement(12208..12630)
                     /locus_tag="ACZPCG_04010"
     CDS             complement(12208..12630)
                     /locus_tag="ACZPCG_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008718797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1284273.1"
                     /translation="MEEAIILKYPISGEDFTRAGEASSDVKRKLKQMGVNPEAIRKVA
                     IAMYEGEINMVIHADGGEITVEITPEKIDMILDDKGPGIKDVELAMQAGYSTAPDEIR
                     SLGFGAGMGLPNMKKYSDQMEIDTRIGEGTKITMTVYI"
     gene            complement(12685..13032)
                     /locus_tag="ACZPCG_04015"
     CDS             complement(12685..13032)
                     /locus_tag="ACZPCG_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HIR1284274.1"
                     /translation="MTVRDVREVLGARVLAGEEHLDREVRSACGSDMMSDVLAFSKDH
                     SVLLTGLCNPQVIRTAEMLDIVCIIFVRGKKPDESMLEMARERNLVVLATGHRMFSSC
                     GMLYQAGLNSGAI"
     gene            13253..13747
                     /locus_tag="ACZPCG_04020"
     CDS             13253..13747
                     /locus_tag="ACZPCG_04020"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE family
                     protein"
                     /protein_id="HIR1284275.1"
                     /translation="MACKKQTVPFRGTPEQEAALKAVIADLKSQPGALMPVMQKAQEI
                     YGYLPIEVQTIISDEMEVPLEKVYGVATFYAQFALQPKGQYKISVCLGTACYVKGSGE
                     IFHKLEEILGITNGECTPDGKFSLDSCRCVGACGLAPVMMINGEVYGRLTPDDIPGIL
                     AKYN"
     gene            13751..14308
                     /locus_tag="ACZPCG_04025"
     CDS             13751..14308
                     /locus_tag="ACZPCG_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1284276.1"
                     /translation="MMPEISLNILDVAENSTRAGASLVTINVTADTDADRLTIQIGDN
                     GCGMSEEQLSHVTDPFYTTRTTRKVGLGVPFFKMAAESAGGSFSIHSQIGKGTEVTAE
                     FGLSHIDRMPLGDINSTIHNLIVYHPDTDFCYTYTYNGASFTLDTRQMKEILGGIPLN
                     TPEVSAYILEFLNENQKETDGGASL"
     gene            14344..14721
                     /locus_tag="ACZPCG_04030"
     CDS             14344..14721
                     /locus_tag="ACZPCG_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HIR1284277.1"
                     /translation="MKTLAELQAIRERMQSQVNLRAEDHNHTRVVVGMATCGIAAGAR
                     PVLNTFAQEIQSRGLTDRISVTQTGCIGLCQYEPIVEIMEPGKDKITYVKMNPDKALE
                     VIERHLVGGHPVEKYTMGAAGLK"
     gene            14736..16526
                     /gene="nuoF"
                     /locus_tag="ACZPCG_04035"
     CDS             14736..16526
                     /gene="nuoF"
                     /locus_tag="ACZPCG_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006778966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HIR1284278.1"
                     /translation="MYRSHVLVCGGTGCTSSGSPQIMDTLKNELKRQGLEEEVAVVET
                     GCHGLCALGPIMIVYPDATFYSMVQPGDIPEIVSEHLLKGRVVTRLLYQETVSPTGGI
                     KALSDTDFYKKQHRIALRNCGVIDPENIEEYIGTGGYQALGKVLTEMTPEDVIQTLLD
                     AGLRGRGGAGFPTGLKWKLCRQNDADQKYVCCNADEGDPGAFMDRSVLEGDPHAVLEA
                     MAIAGYAIGANQGYIYVRAEYPIAVERLQIAIKQARDMELLGKNIFGTGFDFDIDLRL
                     GAGAFVCGEETALMTSIEGKRGEPRPRPPFPAQKGLFGKPSILNNVETYANIPQIILN
                     GPEWFASMGTEKSKGTKVFALGGKINNTGLVEVPMGTTLRIVIEEIGGGIPGGKKFKA
                     AQTGGPSGGCIPAEHFDIPIDYDNLIAIGSMMGSGGLIVMDEDDCMVDIAKFFLEFTV
                     EESCGKCTACRIGTKRMLEILTKITKGQADMADLDKLEELCYYVKNNSLCGLGQTAPN
                     PVLSTLRYFRDEYEAHIKEKRCPAGVCKALLSYNIDRDKCRGCTLCARTCPAGAIIGS
                     VKNPHIIDQNKCIKCGACMEKCKFGAIYKK"
     gene            16544..18289
                     /locus_tag="ACZPCG_04040"
     CDS             16544..18289
                     /locus_tag="ACZPCG_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002596038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HIR1284279.1"
                     /translation="METIKLKINGIEVTAPKGSTILEAARLAHIEIPTLCFLKEINEI
                     GACRVCIVELKNGKLVTSCVYPAEDGMEVFTNTPKVMDSRKKTIQLLLSNHNRSCLSC
                     VRSGHCELQELARELGVEDEGYYDGETTPSEIDTSAAHMIRDNSKCILCRRCVAVCSE
                     VQGIGVIGANNRGFATEIGSAFGMGLGETSCVSCGQCIAVCPTGALQEKDCTSEVFAA
                     IADPSKHVIVQTAPAVRAALGEEFGLPIGTNVEGKMAAALRRLGFDRVFDTDFSADLT
                     IMEEAHEFLDRVKNGGVLPMITSCSPGWVKYCEHYFPDMTENLSTCKSPQQMFGAIAK
                     SYYAEKMGIDPKDIVSVSVMPCTAKKFEIGREDEAANGVPDVDISITTRELARMIRKA
                     RIRFLDLPDESFDEPLGLGSGAGVIFGATGGVMEAALRTAVETLTGEELPKLEFHEVR
                     GTAGIKEATYHVADMDVKVAVASGLGNARELLKKVKSGEADYHFIEIMGCPGGCVNGG
                     GQPQQPGYIRNTVDIRGLRAKVLYDSDEANTIRKSHENTAIKELYATYLGEPGSEKAH
                     HLLHTSYVKRSINQH"
     gene            complement(18392..20920)
                     /locus_tag="ACZPCG_04045"
     CDS             complement(18392..20920)
                     /locus_tag="ACZPCG_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006771095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HIR1284280.1"
                     /translation="MDTRLKNSRSRHWIGVLLIVILVIVSAAATVGFYPYMKQKAVNQ
                     SSLSRQEWENDTGRFDNLATQVMNFSYVIWHQQKQEEKGGILTYSQTFLPGLDEKIAK
                     AEKDAYMEEDITYEGEYELPEQTDQLEMMHEAQSQIEELGRRWEEMYERYNGTLIRYM
                     VTGPDGQCLRSNVIDAESVFSRGIGANEIRFTAEFASGGRLSISDIAGDGDSCNRMFQ
                     SMTSYEFYDPIETRLGSQYRSSGVEFQGPKDMKIHFICDLSNAGSSFYGSDIEETGVR
                     SWDYQSGGGYFSVVCAIAAVITLTALALPGIRSLEIGRSALCRLSFEPLCASGTLWFA
                     TVISSIPVDLIHATMEGRLSREWLQAGFTDGAAHMMTLLINVMFWIIIYGILYWGITC
                     IRAVFTLGLWRYFKERTWLGRFLRFCKRWTLKGLDLFSNTDWEGKSTRIIGKAVIANF
                     IILALISCLWFWGIGALVIYSLVLFFLFKKYWGKMEEKYQLLLKGINAMAEGNLDVEF
                     DENLGIFEPLKEQLSHIRDGFKKAVDQEVKSERTKSELITNVSHDLKTPLTAIITYIN
                     LLKQENLSEEERSSYIQVLDRKAMRLKELIEDLFEVSKANSGTVTLHLEEVEIVSLLK
                     QVRCELADKIEKSGVDFRFQLPEEKIYLHLDGQKTCRIFENLMVNITKYAMKGTRAYI
                     QIRREENSYVAVSMRNISEHELTVSPDELTERFVRGDESRNTEGSGLGLAIARSFTEA
                     QGGTMKLEAEDDLFKVTVRWKEEKMEKPPVHTEPVEEADPLKSADLQPVDPQVSDVQP
                     VSSQPSGLQPSGSRPPVPASFTWWDPEKNEMLEEQEEEKETDEP"
     gene            complement(20892..21602)
                     /locus_tag="ACZPCG_04050"
     CDS             complement(20892..21602)
                     /locus_tag="ACZPCG_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008718823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1284281.1"
                     /translation="MSGDRILLVEDDKEIREGVEIFLKSQGYEVFQAADGLEGLEILK
                     NQPIDLAIVDVMMPRMDGITMVARLRESFDFPVLFLSAKSEEVDKILGLNMGADDYIT
                     KPFTPMELLARVNSHLRRYHRFMDRLHSRAEESARIYRAGGLEVNEDTVSVTIDDRPV
                     KVTPIEFKILLLLIRNPGRVFPADEIYERVWNERAINTDTVMVHIRNLREKIEVNPKE
                     PKYLKVVWGVGYKIEKQP"
     gene            complement(21751..>22158)
                     /locus_tag="ACZPCG_04055"
     CDS             complement(21751..>22158)
                     /locus_tag="ACZPCG_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284282.1"
                     /translation="GHHVLKNSITYENKAKGIDSNSCPDIEIYNNVSFNNEGPNIAIY
                     TANRADTAYVAEGNISYGTGEGHSEDIQLKGQDASSIYSETNYFYHEDTKAYENSKGE
                     KVTDDWFVSLDTTVMPKRAEDGSLLLNGLLERK"
BASE COUNT         5391 a         6134 c         4777 g         5856 t
ORIGIN      
        1 aaaacattta cataagatat ttctttatat atcttcctgt atatgatacc gggctttctg
       61 atacttcttc cggcgttccc tttgcgatca ccgtaccgcc cttgtctccg ccttcgggtc
      121 ctatatcaat aatataatca gctgtcttga tcacatccag attatgttca atcactacta
      181 ctgtatttcc accatcagat aagcgccgga gaatctcaat cagtttatga acatcggcaa
      241 agtgaagccc tgtggtgggc tcatccaaaa tatagatggt ccttcccgtt ccgcgcttgg
      301 aaagctccgt cgccagcttg attctctggg cttctccgcc ggaaagttct gtagagggct
      361 gtcccagacg gatatatcca agccctacat cataaagggt ctgaatcttt ctggtaatag
      421 aaggtacatt ttcaaagaac gtcagagcat cctccaccgt catatttaac acatcataga
      481 tgttcttccc cttatacttc acctccagag tctccctgtt atacctcttt ccgccgcaga
      541 cctcacatgg cacatataca tccggaagaa aatgcatctc aatcttgatg atcccgtcgc
      601 cggagcaggc ctcacagcgt ccgcccttta cgttaaagct aaaacgtccc ttgctgtatc
      661 ccctggcttt ggcatcactg gtagaggcaa acagatcgcg gatcagatca aatactcctg
      721 tgtaggtggc cggattagat ctgggagtgc gtccaattgg agactggtca atggcaataa
      781 ccttgtccag ctgttccgtg ccttctatac acttatgctt tcccggtatg gttctcgccc
      841 ggttcagctt tttcgccagg gctttataca gaatctcatt tacaagagaa ctctttcctg
      901 atccggatac acctgtaacg caggtcatca ctcccagcgg gaaggataca tcaatatttt
      961 tcagattgtt ctccgccgcc tttcgtactg taatccagcc agcagggagc cgacgctttt
     1021 ccggcacggg aatgcggatg cggccgctta agtaggcccc tgtaatagaa tccgggtttg
     1081 ccataatttc ttctgccgta cctaccgcta cgatctgccc tccatgttca ccggccctgg
     1141 ggccaatatc tacaatacaa tccgccgccc gcatggtatc ctcatcatgc tccactacca
     1201 ggacactgtt gccaagatcc cgcaggtgaa gaagggtctt taagagcttg tcattgtccc
     1261 tctggtgaag gccgatgctt ggctcatcca gaatgtatgc cacgcccacc agaccggaac
     1321 cgatctgcgt tgccagacgg atgcgctggg cctctccgcc agataaggtg cccgtagccc
     1381 gcgaaagaga cagataatcc agtcccacat caatgagaaa gctgatcctg gcacgaatct
     1441 cctttaaaat agactctccg atggtctgct gcataggggt cagcttcagc tcatccataa
     1501 actccttaaa cttcacaatg gacatattag tggcttcata gatgttccta tcccccaccg
     1561 ttaccgccag agattccttt ttcagtctct gtccgcggca gaccggacag ggcgtaatgc
     1621 gcatatacgt ctcatattct gccttggagg cctcagaaaa ggtttccttg taacggcggc
     1681 tgacattttc aatcagcccc tcaaaggcca catcatagac gccttcgcct ctctggcttt
     1741 tatagcgcac ctttacctca tgccctccgg tgccgtacag caggatatca tggatttcct
     1801 tgggatatgc ctcaaagggg gtatccagat caaagtggta ctcttttgcc aaagcgtcca
     1861 ggatcgcccg ggtaaagctc cccttatccg tacaggactg ccagcctaaa actacgatag
     1921 cgccctggtt aatcgaaaga gacggatctg gaatcatcag gttttcatca aactccatct
     1981 tatagcccag tccaaaacac tccggacagg ctccgaaggg attgttaaag gaaaaactcc
     2041 tcggctctac ctcatcgata ctgatattgc agtccggaca ggaaaagctc tggctaaaat
     2101 taactggctc ccctccgatc acatctaccg tcatcaggcc gccgcttaag gccataaccg
     2161 tctcaatgga gtctgtcaga cgattttcaa tcccttcttt cactaccaga cggtctacca
     2221 cgatctctat attgtgtttg atattctttt caagatgaat ctcctcagaa agctcataga
     2281 gatttccatc aatccgtaca cgcatataac cgctttttct ggcctgctcc agaaccttta
     2341 catgctctcc cttccgtccc cggaccacag gcgctaacag ctgcagcctt gtacgctccg
     2401 gcatagccat aatctggtct accatctgat caatggtctg cttgtgaatc tcccttccgc
     2461 acttcggaca atgggggatt cccactctgg cataaagaag cctcatataa tcataaatct
     2521 ctgtcaccgt acccaccgtg gagcgcggat tacggttcgt ggatttctga tcaatggaaa
     2581 tggccggaga caggccttca atgctttcta catccggctt ttccatctgt cctaaaaact
     2641 gacgggcata ggaggacagg gattccatat agcgtctctg tccctctgca taaatcgtgt
     2701 caaacgccag tgaagatttt ccagagcccg aaaggcccgt caaaaccacc agctcatctc
     2761 tgggaatatc cacggaaaca tgcttcagat tatgttcgct ggctccccgg atcttaatgt
     2821 attgctttgc catggcgtaa actcctaaaa tcatttgatg caatcagtat atcacatcaa
     2881 atcctgtttt tcaaacattt gttcgatatt acaaaaaagg aaattcctat ctgcacagac
     2941 attcccttcc tttaaaaacc tcgcacataa ttccggctcc cagacgaaat ccgtcacaga
     3001 aggtctggct tttttctata taagtcagct ccagataatg ctctaacagc gcatcaaaac
     3061 tctttcggct ttcatcgtcc agcttttttg aaaattcctc gatttcctga actgactgtc
     3121 tgcacagccg gtcatactcc tcatttccct gaatcacgtt ttctgccgga gttaaatctc
     3181 ccgcatacag ctgctccaaa atactgctca tctgtcttac ctgcctttct taatttagta
     3241 catatgttcg ggtatttcat cataacacag ccccggagca aatgcaatcg aacaaaacct
     3301 tacgattgca cccgtccggg gctgtaatct ttcttttatt cttcctcttt caaggtattt
     3361 tccccatctg cgctctgttt ttcaaaaatc tccataaact cttccccggt gatcgtctcc
     3421 ttatccagaa gataagccgc aatctcatgg agctttgtct cgttttcctt taaaatgtga
     3481 attgcctttt catgctgctc ccggacgatc tgaactacct tttcatcaat ccgtttggct
     3541 gtttccggag agcacatcag agaggtatct cctcccagat actgattatt caccgtttcc
     3601 atggcaacca tgtcaaattc atccgtcatg ccatatcgcg ttaccatggc tcttgcgatc
     3661 ttggttgcct gctcgatatc attggaggct cctgtggtaa tagaatggaa aataacttcc
     3721 tctgccgccc gtccgcctgt aaaggtggca atcttattta acgcctcatc ccggctcatc
     3781 agataccgtt cgccctgatc aacctgcatg gtatatccaa gggctcctga agttcttgga
     3841 attatggtaa tcttctgaac gggagcgctg tgggtctgac aggccgctac cagagcgtgt
     3901 cccacctcat ggtacgccac gatccggcgc tcatccggcg gaatcaccgc gtttttcttc
     3961 tggtagcctg caatgacggt ttccacactt tcctgaagat cctcctggca caccgccttt
     4021 cttccaaagc gcaccgcccg cagcgcagcc tcattgacaa tatttgcaag ttctgcgccg
     4081 ctggctcctg ccgtagccct ggcaatggca ttataatcta catcatcgga catcttaacc
     4141 ttctgtccgt gtacctttaa aattgcctct cttcccttta aatcaggcag ctctactggt
     4201 acccggcggt caaaccgtcc cggacgcagc agggccggat ccagggattc cggacggttg
     4261 gttgccgcga ggattaccac gcccttcttt ccgtcaaagc cgtccatctc tgtcagaagc
     4321 tgatttaagg tctgctctct ctcatcattt ccgctcatac cgccgccgtc gcgcttcttt
     4381 ccgatggtat cgatctcatc aataaacaca atacagggag ccttttcatt ggcctgctta
     4441 aagagatctc tcaccttggc agcgcccata cccacaaaca tctctacaaa ttctgaaccg
     4501 gaaatggaaa aaaacggaac atgggcttcg cctgccacag cctttgcaag aagggttttt
     4561 cccgttccgg gagggcctac caaaagagcg ccctttggca gattggctcc gatatccgca
     4621 tatttttccg gattgtgaag gaaatctaca atctccttta aagcgtcctt ggcctcttcc
     4681 tctccagcca cgtcacgaaa cgtaacgccg gtttcatttt ccccgtaaat ctttgcattg
     4741 gacttgccaa aggtcatggc gtttccgccg cccatgcgct ttgccatcat ccgcgccata
     4801 atctgtccga tgactacaaa catcaggatt gggaaaatcc atgtcatcag aaaattaacc
     4861 aggggggatt ccttgtgcgg gatctgtttg gtataaacct ccacccctgc cgctgaaagc
     4921 ttggcctcca gttcatcttc cgtaaccata tttcctgtct tgtaggttat ggcagaccac
     4981 tggggtctgg ctccctcctc cggctttaac gaataaacaa tctctgtatt gtcactgctt
     5041 acatatgcct ctttgatatt tcccgcatcc agctgagccg taaactcact gaacggcacc
     5101 tccatggagc gttccatcat agacgggaat acaagtgcat ttaaaaccat cacgaccaga
     5161 aggacgatga tgtaataata gagaaacggc tttttatttc cgtcatcgct gttttttata
     5221 cccatacagt ttcttccttt cccagaccgg ctgcgcatat tttaccaccg ccgctgtata
     5281 tagctaagat aataccaatt ttacctttta tcaatagact ttatcaaata ttctcataat
     5341 ttattggaat ttaatcaaaa aatcccgcag ctggcacata cagcgccgaa gccgcaggat
     5401 tccctgtttt ataatacctt gcttaaaaaa ctctttaaac gctcattttt aggatttgtg
     5461 aaaaattcct caggagctgc ctcctccata atataaccgc cgtccataaa cagaacgcgg
     5521 gtagctacct ctctggcaaa gcccatctca tgggtaacga ccaccatcgt catcttctct
     5581 tctgccagat cgcgcataac gtttaatact tctcctacca tctccggatc aagggctgag
     5641 gtaggctcat caaagagcat cacatccggc ttcatgcaga gagctctcac aatagcgatt
     5701 cgctgcttct gtcctccgga aagctggctg ggataagcat gggcacggtc tgccagcccc
     5761 acgcgctcta acagccgcat ggcctctttt tctgcctcct cctggctgat tccctgaagc
     5821 tttacagggc cgattgtaag atttttcata atgtccatat gagggaacag attaaactgc
     5881 tggaatacca ttcccatctt ctggcgatgc ttatcaatgt ttgtctttgg atctgtgata
     5941 tcggttccct caaagaaaat atggcctctg gtgggctcct ccagacggtt taagcaccga
     6001 aggaaagtac tctttccgga gccggaaggt ccgatcacga aaaccacatc gcctttattg
     6061 atatctacac tgatatcctt taatacatct acggctccaa agcttttccc aagaccctga
     6121 acatggatca gcgcttcttc tttcttatta ccgctcattc tgacgcagcc tcctttccag
     6181 aatctggatc agttttgtaa agatcaatac aatcgtaaga tataccagcg ccactgcgat
     6241 caagggcata aatgcgctgt aggtcctgct tcgtataata tcgccgccct tggtaagatc
     6301 ctgaatggca atataaccgg aaacagaggt ttcctttaaa agagaaataa actcattaca
     6361 cagggtagga agcacaacct taaatgcctg aggcataatg atgtagcgca tggtctgaaa
     6421 atagttaaag ccaaggcttc gtcctgcttc catctgtccc ggatcgatcg acatgatacc
     6481 gcttcggaag atttcagcta cataagcgcc ggaattgatt ccaaaagcca taatcgccac
     6541 gatcaccttg tcgatgcgtg cgctgccgaa tacaacaaaa tagatgatca gcagctgtac
     6601 tacaaccggc gttccgcgga ttacggtcag ataaatctga cagatcacat tgaggaattt
     6661 aagttttccc gttttatcat gggtggagcg gatcattccc acaataaagc ccagcacaat
     6721 acccataagg caggcaaagc aggtgatgat cagagttgtc tgcagaccat ctgtaatata
     6781 acgccaccga tcctcagata taaaatttaa ataaaatgca tttgtaaaat tttcccacat
     6841 tccacttttc cctttctttt atgaatgtgt aagccagaac agttccttcg ttccggaatc
     6901 tgtctcacct ctgcttgctg ctctgtgacg ggacgcaggc agacagccgc ccgcagcaga
     6961 tttcagacag gctccggaaa agacccggcc cgcacctggc ggaccggata ttggctgatt
     7021 cttattctgc tgtgatgtat tttgctacga tctcatccag agtcccctcg gactctaact
     7081 cagaaagtgc gccgttgatc ttgtcaagga gttctgtatt tcccttctta acagcaatcg
     7141 catattcctc ctctgtaaag gcctcatcta acatcttgat gccctcagtc tcagctacga
     7201 acgtctttgc cggctcgcca tcgatcacaa ctgcatcgat cttgccctgg cttaatgcct
     7261 gaacagcctc aaaacccttg ttgtaacgct ctacggtcgt tccctctgcc tccagatcgg
     7321 aaacatagat atcgccggtg gttcccagct gaacgcctac tacaacaccc tttaagtcat
     7381 ccgggcctgc aatcgcgctg tcttctttta cgatgatcat ctgggatgcc tttgcgtaag
     7441 tatcagaaaa atctacagac acttttctgt cctcagttac ggtcatgcca gccagaccca
     7501 tatcggcctt tccggaagta atttcaggaa taacggaatc aaatgctaca tcctcgatct
     7561 ctaactccat gcccatctta tctgcaatgg cttttgcaat ctctacgtca atgccaacga
     7621 tctcgccgcc gtcatggtac tcataaggcg ggaactctgc gttggttgcc attaccagaa
     7681 caccgcctgc tgcttcggtc tcttccttag ccgctgtggt ctcttctgcc tttgggtcct
     7741 ctgccttggt atcctctgct tttgcctctg tcttagctgc tgtggtctct gcagcagaac
     7801 cgccgcaggc agttaatgat gctgccatta aagctgctgt aaatgcaagt acatattttt
     7861 tcataatgct ctctcctctt tctgctttgc ttaatcatgc atatgtatgc ataaatataa
     7921 attcagtaac gttttatatt atagacactt ttttcatttt cgcaagaggt aatttttgat
     7981 tcacctcatt ttgtccagtt tttactgtaa aacattgctt cgggccgcct gaatcagatg
     8041 ctttgccagt actgcagtgg ttacggctcc tacgcctccc ggcactggtg taaggagaag
     8101 gttccggctc tccatggatg cctgatccac atcaccgcag agttttccgt tttcgtccac
     8161 attaatgccc acatcaatca ccaccgcgcc atctgatacc cagctttctt ccaccattcc
     8221 cgcctttcct gccgcagcta ctaagatctc agcctgccgg cagatttcag gaagctcttt
     8281 ggtgcgcgta tggcagatcg tcacggtagc gttttcctta aggagcagca tggacagcgg
     8341 ttttcctatt accatgctcc gtccgatcac tgccgcacgc tttccttcta tagaaatgtt
     8401 atttgctttc agtatttcga ttaccgcctc tgcggtacag ggagcaaaac cggagggatc
     8461 tccggaaaac acctttgcaa tgtttaccgg tgaaatacca tccagatcct tttttggatc
     8521 gatcatggct tcaatctcct tctcctttat ctgcctcgga agcgggcgca gaagcaaaat
     8581 gcccgtaaca tccggatcct tattgatctt ctgaaaggct tctttaaact cttcgtctga
     8641 aatatccttc ggaaatacaa agatctgcac cctcagccca aaggcttcca tcttttttac
     8701 agcacttctt tcataggaca tatcgtccgg tttttctcct acccggataa tggcaagctt
     8761 gggaaccgat ccctgaagct gttttatttc ctccgcagcc cactctctga tggcagcaga
     8821 aacaggagcg ccctttaaca attccatttt catttcctcc tcatgtttaa tggttctgtg
     8881 tcatcagccc tgaagctgct ttactatttt ttcatagatc cgatccgcag cgtctgtccc
     8941 ttcttttgta agacgcttcg cttcacggtt cagttcttct gcccttttac gatccttcat
     9001 ggaacgggta ttgatataca cgttcatcac agcccctaaa atggcagtac gggcaaactg
     9061 aacgcctacg cccacatcac tgaccgccat aacgcttccc ttctctgcca gttcctttaa
     9121 aatccccagc atttctgctg ccttttccat gatttccaga ggaacatagc tggcctgtac
     9181 aagccgttcc tccattacct tttccttata tgccttttct tcctccgttg atgaggggag
     9241 gcggtaacat tctgccagag gggcaaacac ctctgcatcc ttatctgcca gcatcagaaa
     9301 ttcctgctga agacgtttca gctctctttc atagttcagc atatcctgct ctgcctgcgc
     9361 atacctcttc tttcccaggg tcaggtgtac caccatctgt cccaggctgt ctcccagtgc
     9421 gcctgcaaga gcagaggctc ctccgccgcc cggtacgggg gctttggatg ataataattc
     9481 cagataatct ttaatctgtg cttctgctgt catgatcata tgcatccttt ctactgtact
     9541 ttaatgcctt aatcctgtcc cccatcatac cacgacctgc ctctgtgcca caaggacttt
     9601 tatacttgag tttccgcatt ttgcagtgcg ctctggcatc tggtgttcag gacgactttg
     9661 aaggccataa gaaaaccgga gccctgaaca ccagacgccg gagtgcggtg taaagtgtgg
     9721 aaactcaagc ttttataaaa gagatttcct taaaaaagcc ctctgcgcag tgtatccaca
     9781 acagccttgg cagaccgctc tgcagccggc agcgtgaatc cccgttcgtt gatatgctcc
     9841 agataatgtg cgtctgaatt actgatgatg cggcagtgct ccagataagg gtgttcccgt
     9901 ttcagagaat gaagcttctt catatccttc agttccacag tctgaaagga gctgtccgga
     9961 ggaataaagc ccagattggc aatgaggctg ttggaatttt tatcaatatg ggccggaaac
    10021 atgacacccc cgtaggaagc cgccttttcc cacaacccct caaaagacaa attaacggca
    10081 ttgataagca gacaggattc ttccccgtag acagaatcat tttcatcata aatctgctgt
    10141 tttccaaaaa tttccgggcg attttttacc ttaatgagac ggctgtacac aaagcggtca
    10201 aattccatcg cctgataaag ctccggaaac agacagactg cgtggacttc ctctgaagtt
    10261 gtcagttcca tccccggaat cccgatgatc ccgtattcct tcgccgcagc caggaacgca
    10321 gggcagtttt tacagctgtt gtggtctgtg agagccactg catccagccc ttttaacgct
    10381 gccattcccg caatattggc cggcgtcata tcatcatccc cacagggcga taagcaggaa
    10441 tggatatgaa gatcatatgc cagctctgtc attttaacag ctcctttaca gccagggccg
    10501 tctcaaatac cggttcctct gtccgcagca ccgtgatccc ctgaagagca gccttcttca
    10561 cagcagcctc atccagcatg gcaccctctg ccagaatcac acaggccgca tccgcaagag
    10621 ctgccactgc cagcgtattt aagtttccca ttaccgtaac ccaggcacag cctgccggag
    10681 cccgtcccat ggcaatgctt aaaagatcgc agcaaaaggg ctttgtgatg ctccggtctg
    10741 tatcgtcccc tatattaatc tcctgaaaaa catttttatc aatcagttcc tgtacggtca
    10801 ttcttcctcc ccctcttccc ggctgtccag agaagacatc tcatccgaaa tccgccgtat
    10861 gcactctttt aatgtattta aggtatcctg gctgctttta tccttgctgt gaagatcgtc
    10921 tgccagtatg gctacctcct cagaaagcct gtggagattt tctctcagat aatacacgca
    10981 gtcggtctca ctggcctctc cccgaacaat gtcctccgcc agggccttac aggtaggagc
    11041 tccgcaggaa ccgcagtcca gtcccggaaa tttcttcaca agccgctccg cctgatttaa
    11101 acgcagaaag ctttccatca tattatttcc cagattaaat acgggttcaa actggatctg
    11161 cgttgtccat tttaccgcat ccagctcacc atcctccata tggtttctgg caacaggcat
    11221 atatttgcgc aggcgcttta gcttcacctc tgccacatag ggattttcca ccgtaagcac
    11281 accgcccaca cagcctccgt tgcaggcgtt cagctccaca aatttcagat tggtaaactt
    11341 ctgatcttcc atatcctcta acacacggat cacattttca ataccgtccg ctgccagata
    11401 attttccgta aacagcccgc tggcttcccc gccgctgtgc ccccagctga tgccgatttt
    11461 tccggaagtt ccaatagctg gataatcctc tcccgctacc gacttcatgg ctgctaacag
    11521 tctgggatac acatccttga tagcaagcac cttgtccacc tggctctttt ccgtaccaag
    11581 aggcgctctc acataggtta ccttggaggg gcagggagaa ataaacataa ttcctatctt
    11641 ctctctggga agccccgttt tctccatggc ccttctggcc gccagaatag ccgctacctc
    11701 taccggggga tttaaaggaa gcaggtgagg aatcagggat ggaaaacgca cccggatcag
    11761 ccgcaccaca gaaggacagg cggtactgat caaaggaagc tgttcctcat ggtcggatat
    11821 gtactgtctg gtggcttcac tgaccagctc tgccgccgcg ctgacctcga atacatcatc
    11881 aaagcccata cgaagaaggg cctttaacac aatattagag tcatccagat tgttgaactg
    11941 gctgtacaga gacggcgccg gaagcgccac cgtatattcg tactgattca tcgtatccag
    12001 cttatcgtag gttgcgtgtt ttgcatgatg agggcagacg cggatgcact ctccgcagtc
    12061 tatgcagaac tttttattga tgcttgcctt tccccctcgg acgcggatgg cctctgtagg
    12121 acagcgctta atgcagttaa tgcagccttt gcacaaagaa gggtcaagcc gcacggaatg
    12181 atagaattta tccattcctc caccccctca gatatatact gtcatggtga tctttgttcc
    12241 ctctccgatc cgggtatcga tctccatctg gtctgaatat tttttcatat tgggaagccc
    12301 catccccgct ccaaagccca gagaacggat ctcatccgga gccgtggaat atccggcctg
    12361 catcgccagc tccacatcct taatccccgg ccctttgtca tccaggatca tatcgatctt
    12421 ttccggagta atctctactg taatctcccc tccgtctgca tggattacca tattgatctc
    12481 cccctcatac atggcgatgg ccaccttccg gatggcctcc ggattcaccc ccatctgctt
    12541 gagcttgcgc tttacatcgc tgctggcttc ccctgcccgt gtaaaatcct ctccggatat
    12601 gggatatttt aaaataattg cttcttccat agaaaatctt tccttcctga atttccatat
    12661 atcgtcgtat cagcgcagca ctctctagat tgccccgctg ttaagccctg cctgataaag
    12721 cattccgcag gaagaaaaca tcctgtgtcc ggtggcaaga accacaagat tcctctccct
    12781 tgccatctca agcatacttt catccggctt ttttcctctc acaaatataa tgcacacaat
    12841 atcaagcatc tctgctgtac ggatcacctg gggattgcac aatcctgtca gaagtacgga
    12901 atgatccttt gaaaatgcca gcacatcgct catcatatca gagccgcagg cagaccttac
    12961 ctccctgtcc agatgctcct ctccggccag caccctggcg cctaatacct ctcttacatc
    13021 tctcactgtc atacgcgttc tctcctacct gtttggttct gtgtttccgg ctactgtcag
    13081 catatcattt atccccaatt ctttcaataa atatgtctct gtttttgcac attttatccg
    13141 caattccaca caaagttttt gacacatttt cccagaaatg tagatttttt tgactatatg
    13201 tgttagaata ttaacacatt gcataattta caacgacgag gaggtaaaaa cgatggcgtg
    13261 caaaaaacaa acggttccct ttcgaggaac acctgagcag gaggccgcgc tgaaagcggt
    13321 aattgctgat cttaaaagcc agccgggcgc tctgatgccg gtgatgcaga aggctcagga
    13381 aatttatgga tatcttccca ttgaagtgca gaccatcatc tcagacgaga tggaggttcc
    13441 gttagaaaag gtttacggcg tagcgacctt ttatgcacag tttgccttac agcccaaggg
    13501 acagtataaa atttctgtct gtctgggaac agcctgctat gtgaagggtt ccggggaaat
    13561 ctttcacaag ctggaggaaa ttttagggat taccaacggt gagtgtacac cggacggcaa
    13621 attctcactg gattcctgcc gctgtgtagg cgcctgcgga ctggctcctg ttatgatgat
    13681 caacggagaa gtttacggcc gtcttacccc cgacgacatt ccgggcattt tagccaagta
    13741 taactagcct atgatgccgg aaatttcctt aaatatactg gatgtagcag aaaattccac
    13801 acgggcaggc gcctctcttg tgactataaa cgtcacagca gatacagacg ccgaccgtct
    13861 gaccatacag atcggggata atggctgcgg catgagcgaa gaacagcttt cccatgtaac
    13921 agaccccttt tacaccaccc gcacaacgcg gaaggtagga ctgggcgttc ctttttttaa
    13981 aatggctgcc gaaagcgccg gagggagctt ttccatccat tcccagattg gaaaaggcac
    14041 agaggtgact gccgaatttg gcttatccca cattgaccgt atgcctctgg gggatataaa
    14101 cagcaccatc cacaacctga tcgtgtacca tccggacacg gatttttgct atacatatac
    14161 atacaacgga gcttccttta cccttgatac caggcagatg aaggagatcc ttggcggtat
    14221 tccattaaat acgccggaag tatccgccta tattttggaa tttctgaatg aaaatcagaa
    14281 ggaaaccgac ggcggcgcat cgctgtgacc gctgcccctt agacagaagg aggtttataa
    14341 cccatgaaaa ctcttgctga attacaggcc atccgcgaac ggatgcagag ccaggtaaac
    14401 ctgcgcgcgg aagaccataa ccatacccgc gtagtagtag gcatggccac ctgcggaatc
    14461 gccgcaggtg ccagacctgt tctcaatacc tttgcccagg agatccagtc cagaggcctt
    14521 acagaccgca tcagcgtaac tcagaccggc tgcatcgggc tgtgccagta tgaacccatt
    14581 gttgagatca tggaacccgg caaggacaaa attacatatg taaaaatgaa ccctgacaag
    14641 gccttagagg tgatcgagcg ccatctggtc ggcggacacc cggttgagaa atataccatg
    14701 ggcgctgcag gattaaaata aaggagggac tatctatgta tcgttcacat gtgcttgtct
    14761 gcggcggtac cggatgtacc tcttccggaa gcccccagat catggataca ttaaaaaatg
    14821 aattaaaacg ccagggactg gaggaggaag tagcggttgt agaaaccggc tgccacggcc
    14881 tctgtgccct gggcccgatt atgatcgttt acccggatgc taccttttat tcaatggtac
    14941 agcccggaga catccctgag atcgtatccg aacatctctt aaagggtcgt gtagtaaccc
    15001 gtcttctcta tcaggagacc gtaagcccca ctggaggcat caaggcctta agcgacacag
    15061 acttttataa gaagcagcac cgtatcgccc ttcgcaactg cggtgttatt gaccctgaaa
    15121 atatcgaaga atacatcgga accggcggct atcaggctct gggaaaggtt cttacggaaa
    15181 tgaccccgga ggatgtgatc cagacccttt tagacgcagg cctgagaggc cgcggaggcg
    15241 caggcttccc caccggctta aaatggaagc tctgccgtca gaacgacgca gaccagaagt
    15301 atgtctgctg taatgcagac gagggcgatc ccggcgcatt tatggaccgt tccgtgctgg
    15361 agggcgatcc ccatgcagtg ctggaggcca tggccatcgc cggctatgcc atcggtgcaa
    15421 accagggcta tatctacgtc cgtgccgaat accccatcgc ggtagaacgc ctgcagattg
    15481 ccatcaagca ggcccgcgac atggaacttt taggcaaaaa cattttcgga accggctttg
    15541 attttgatat tgacctccgt ctgggcgcag gcgctttcgt ctgcggcgag gaaacggctc
    15601 ttatgacctc cattgaggga aaacgcggtg agccaaggcc ccgtcctcca ttcccggcgc
    15661 agaagggctt attcggaaag ccaagcatct taaataacgt agaaacctac gccaatatcc
    15721 ctcagatcat cttaaacggt ccagaatggt ttgcttccat gggtacggaa aagtccaagg
    15781 gcaccaaggt atttgccctg ggaggcaaga tcaacaacac cggtctggta gaggttccca
    15841 tgggaaccac cctaaggatc gtaatcgagg aaatcggcgg cggcatcccc ggcggaaaga
    15901 agttcaaggc cgcacagacc ggaggacctt ccggcggctg tatcccggct gaacactttg
    15961 atatccccat tgactatgac aacctgattg ccataggttc catgatgggc tccggcggtc
    16021 tgatcgttat ggatgaggat gactgtatgg tagacattgc caaattcttc cttgaattta
    16081 ctgtggagga atcctgcggc aagtgtactg cctgccgtat cggcaccaaa cgtatgcttg
    16141 aaatcctcac aaagatcacc aagggacagg ccgatatggc tgacctggat aagctggaag
    16201 aactctgcta ctatgtaaag aacaattccc tctgcggcct gggacagaca gcccccaatc
    16261 cggtgctgtc caccctgcgc tatttccgcg acgagtacga ggcccatatc aaggagaagc
    16321 gctgtcctgc cggcgtctgc aaggcgctct tatcctacaa cattgaccgt gacaagtgcc
    16381 gcggctgtac tctgtgcgcc cgcacctgtc ctgccggagc aatcatcggc tctgtaaaga
    16441 atccgcacat tattgatcag aacaagtgca tcaagtgcgg tgcctgcatg gagaaatgta
    16501 agttcggcgc aatctacaaa aaataaaagg gagggaatag acgatggaaa cgatcaaact
    16561 gaaaatcaac ggcattgaag tcactgctcc caagggctca accatcctgg aggcagcaag
    16621 actggcgcat atcgagatcc ctaccttatg cttcttaaaa gagatcaacg aaatcggcgc
    16681 ctgccgcgta tgtattgtcg aattaaaaaa tggaaagctg gttacttcct gcgtatatcc
    16741 tgcggaagac ggaatggaag tatttaccaa tactccaaag gtcatggatt ccagaaaaaa
    16801 gaccatccag cttttattat ccaaccataa ccgctcctgc ctgtcctgcg tacgcagcgg
    16861 acactgcgag cttcaggagc tggcacgaga gcttggcgta gaggatgagg gctattatga
    16921 cggcgagacc acaccctctg agatcgatac ctctgccgcc catatgatcc gtgacaacag
    16981 caaatgtatt ctctgccgcc gctgcgtggc agtctgcagc gaggtacagg ggattggcgt
    17041 catcggcgcc aacaaccggg gctttgcaac tgaaatcggc tctgccttcg gtatgggcct
    17101 tggcgagacc tcctgtgtat cctgcggcca gtgtattgcc gtctgtccta cgggagccct
    17161 tcaggaaaag gactgcacgt ccgaggtatt tgcagccatt gcagatcctt ccaagcatgt
    17221 gatcgtacag accgctcctg ctgtccgtgc cgctttaggc gaagaattcg gccttcccat
    17281 cggcaccaat gtggaaggaa agatggctgc cgccctgcgc cgtctgggat ttgaccgcgt
    17341 atttgataca gacttttccg ctgacttgac catcatggag gaggcacatg aatttttaga
    17401 ccgcgtgaaa aacggcggtg tcctgcctat gatcacctcc tgttctcccg gctgggttaa
    17461 atactgtgag cactatttcc cggatatgac ggaaaatctt tccacctgca aatcccctca
    17521 gcagatgttc ggagccattg ccaaatccta ctatgctgag aagatgggca ttgatccaaa
    17581 ggatatcgta tctgtcagcg ttatgccctg tacggcaaag aagttcgaga tcggaagaga
    17641 ggacgaggcg gcaaacggcg ttccggatgt ggatatctcc atcaccacca gagagctggc
    17701 ccggatgatc cgcaaggcaa ggatccgctt cctggatctg ccggatgaga gctttgacga
    17761 gcctttaggc cttggctccg gtgcaggcgt gatcttcggc gctacgggag gcgttatgga
    17821 ggctgcatta agaactgcag ttgagacact taccggcgaa gagcttccaa agcttgaatt
    17881 tcacgaggtc cgcggcactg caggcatcaa ggaagccacc tatcatgtgg cagatatgga
    17941 tgtaaaggta gcggtcgcct ccggccttgg aaatgccaga gagctgctta aaaaggtaaa
    18001 gtcaggagag gcagattacc acttcatcga gatcatgggc tgtcccggcg gctgtgtcaa
    18061 cggcggcggc cagcctcagc agccgggtta tatccgcaac acggtggata tccgcggctt
    18121 aagagctaag gttctctatg acagcgatga ggcaaatacc atccgcaaat cccatgaaaa
    18181 tacggcgatt aaggagttat atgccaccta tctgggcgag cccggaagcg aaaaggcgca
    18241 ccatctgctc cataccagct atgtaaagag aagtattaat cagcactgat cgaaactaaa
    18301 aacagagatg ccggcgtcac tccggcatcc ccttaaataa aaagcttccc cgcaggacga
    18361 accatctgat cgtctgcggg gaagctttta tttatggttc atccgtttcc ttctcttctt
    18421 cctgttcctc taacatctca tttttttcag gatcccacca ggtaaatgag gccggaaccg
    18481 gcggacgtga accggaaggc tgcaaaccag agggctgcga actgacgggc tgtacatccg
    18541 aaacctgtgg atcgacaggc tgcaaatcgg cagattttag gggatcggct tcctccaccg
    18601 gttccgtatg gacgggaggt ttttccatct tttcctcctt ccagcggacg gtaaccttaa
    18661 agaggtcatc ctctgcttcc agcttcatcg taccgccctg ggcctcagta aagcttcttg
    18721 caatggcaag ccccagaccg gagccttccg tattgcggga ttcatccccc cgtacaaagc
    18781 gctctgtgag ctcatccggg gataccgtta attcatgttc tgaaatattt cgcatagaaa
    18841 cagccacata gctgttttcc tcccgcctga tctggatata ggctctggtt cccttcatgg
    18901 catactttgt aatatttacc atgaggtttt caaagatacg gcaggttttc tgaccgtcca
    18961 gatgaaggta tattttttcc tccggcagct ggaaacggaa gtccacaccg ctcttttcaa
    19021 tcttgtcggc cagctcgcag cgcacctgct ttaacaagct gacaatctcc acctcttcca
    19081 ggtggagggt cacggtaccg ctgtttgcct tgctgacctc aaaaagatct tcgatcagct
    19141 ccttcaagcg catggctttg cggtctagta cctggatata ggagctgcgc tcctcttctg
    19201 acagattttc ctgctttaaa agattgatat aagtgataat cgccgtcaaa ggcgtcttta
    19261 gatcatggga cacattggtg atcagctccg atttggtacg ctcgcttttt acctcctggt
    19321 ccactgcttt tttaaatccg tcccggatat gggaaagctg ttccttgaga ggctcaaaaa
    19381 tccccagatt ttcatcaaac tccacatcca gatttccctc tgccatggca ttaatgccct
    19441 ttaagagaag ctgatatttt tcttccattt ttccccagta tttcttaaaa aggaaaaaca
    19501 gtaccagaga gtagattact aacgccccaa tcccccagaa ccacaggcag gaaatgagag
    19561 ccagaataat aaaattggca atcacagcct ttccaatgat ccgcgtggac tttccttccc
    19621 agtctgtatt ggaaaagaga tccagaccct tcagagtcca gcgcttgcaa aagcgcaaaa
    19681 atcgtccaag ccaggtgcgt tccttaaaat agcgccacag tcccagagta aatacggccc
    19741 gtatacaggt aattccccag taaagaatac cgtaaatgat gatccagaac atgacattga
    19801 tcagcagcgt catcatatgc gccgctccat ctgtaaatcc ggcctgcagc cactctctgg
    19861 ataaacggcc ttccatggtt gcatggatca gatctaccgg aatactgctg atcacagtag
    19921 cgaaccacag cgttccactg gcacataggg gttcaaagga caaacggcac agtgcgctgc
    19981 gtcctatctc cagggaacgg attcccggaa gtgcaagagc tgtgagcgta atcactgccg
    20041 cgatcgcaca tacaacggaa aaatatccgc ctccgctctg gtaatcccag cttctaaccc
    20101 ctgtttcctc tatatcactg ccataaaaac tgctgcccgc attggataga tcacagataa
    20161 aatgaatctt catatccttt ggcccctgaa actccactcc gctgctgcgg tactggcttc
    20221 ccagacgggt ctcaatagga tcataaaact cataggaggt cattgactgg aacatacggt
    20281 tgcagctgtc cccatctccc gctatatcgg aaatacttaa tctgcctccg gaagcaaatt
    20341 ccgccgtaaa ccggatttca ttcgccccga ttccccttga aaacacactt tctgcatcaa
    20401 ttacattgct gcgcagacac tgtccgtcag ggcctgtgac catgtaacgg atcagagttc
    20461 cattgtagcg ttcatacatc tcttcccagc gccgtcccag ttcttcgatc tgagactggg
    20521 cttcatgcat catttccaac tgatccgtct gctccgggag ctcatactca ccctcataag
    20581 tgatgtcttc ttccatgtat gcgtctttct ctgctttggc tatcttttca tccagtcccg
    20641 gcaaaaaggt ctgggaatag gtcaaaatcc cgcccttttc ctcctgcttt tgctgatgcc
    20701 agatcacata gctgaaattc ataacctgtg ttgccagatt gtcaaagcgt ccggtgtcat
    20761 tttcccattc ctgccggctt aggctgctct ggtttacagc tttttgtttc atatagggat
    20821 aaaatcccac agttgctgcc gcagatacga ttaccagtat cacgatcagc agtacgccga
    20881 tccagtgcct gctacggctg tttttcaatc ttgtatccaa ctccccacac cacctttaaa
    20941 tattttggtt ctttcggatt tacttctatc ttttcccgga gattgcggat atgaaccata
    21001 accgtatccg tattgatggc ccgttcgttc cacacccgtt cataaatctc atctgccggg
    21061 aatacccggc ccggattgcg tatcaaaagc agaaggatct taaattcgat gggcgttacc
    21121 tttaccggcc ggtcatcaat ggtaacgctt accgtatcct catttacctc caggcctccg
    21181 gcccggtaaa tccgtgcgct ttcttctgcc cttgaatgca aacggtccat aaagcggtga
    21241 taacgtctca gatgggaatt cactctggct aaaagctcca ttggcgtaaa gggctttgta
    21301 atgtaatcat ccgcccccat gttcagcccc agtatcttat ctacttcctc tgattttgca
    21361 gataggaaaa gtactggaaa gtcgaaactt tcccgcagtc ttgctaccat ggtaataccg
    21421 tccatccgcg gcatcatcac atctacaatt gcaagatcga taggctgatt tttaaggatt
    21481 tccaggccct caaggccgtc ggctgcctga aaaacctcat atccctggct ttttaaaaag
    21541 atttccacac cttcccgtat ctctttatca tcctccacta acaggatccg atccccactc
    21601 atatctgttt cctccttaca tccatatgat aaaatataaa aacaaaaata aggtgtataa
    21661 aatataaaag actttctaaa gattgattgt agcattttaa aaggacagag tcacccccta
    21721 aacagggatt ctctgtcctc tttttatggt ttattttctc tccagaagtc cgtttaacag
    21781 gagggaacca tcctctgccc gtttcggcat cacagtagta tccagggata cgaaccagtc
    21841 gtccgttacc ttctctccct tgctgttttc gtaggccttc gtgtcctcat gatagaaata
    21901 attggtttcg ctgtaaatgc tgcttgcatc ctgacccttc agctgaatgt cctcactgtg
    21961 gccttcccct gtgccatagg aaatattccc ctctgccaca taggccgtat ctgcccggtt
    22021 ggcggtatag atggcaatat tagggccttc attgttaaaa gaaacattat tgtagatttc
    22081 aatatccgga cagctgttgg aatcaatgcc ctttgctttg ttttcgtagg taatgctgtt
    22141 ttttaacaca tgatggcc
//
LOCUS       DBMDUH010000028        21337 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_1552_length_21337_cov_8.047937, whole genome shotgun sequence.
ACCESSION   DBMDUH010000028 DBMDUH010000000
VERSION     DBMDUH010000028.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 21337)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 21337)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21337
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1552_length_21337_cov_8.047937"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="ACZPCG_04060"
     CDS             complement(<1..572)
                     /locus_tag="ACZPCG_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007887414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1284283.1"
                     /translation="MKTIFEKMFGQYRGMRRELYILFWGKAATNMGAMIWPMLTLILS
                     NKLGMNAQEIAKITIAMGVVQFPANLLGGKLADCCNKKKLIIICDLVTVICYLTAAVL
                     PVSMAQILLFFTAGIFQTMENPSYDALVADLSTSENREKAYSLMYLGLNLGIILSPTI
                     GGILFENHLGLAYAIDGLTTLSSTILILLF"
     gene            complement(979..2223)
                     /locus_tag="ACZPCG_04065"
     CDS             complement(979..2223)
                     /locus_tag="ACZPCG_04065"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1284284.1"
                     /translation="MIYRLRVKFVVINMTIVVLMMSVVFGLVYHFTRADLEEKSIRMM
                     EKIANRPFQIEMPGEQSEQIRLPFFILHLGTGGELIASSGGYFDLSDQEFLMDLIVRA
                     RSSHQKVGVIKEYGFRYYKTNTPYRYSLVFADISSETAALKGLVRTCALIAGLSFILF
                     LAISIWLSGWAVRPVEKAWKQQRQFVADASHELKTPLTVIMTNAELLQSSGCGEEEQR
                     RFSEAILAMSRQMRGLIEQMLDLARADSGQAEAAFEQVDLSRTAEDSLLLFEPLYFEQ
                     GLTLYAEIEEGILIWGLKDRLSQLLDILLDNACKYSSQGGTTWVKLKKQGKSHCILTV
                     ENQGPSIDPEDLSQIFKRFYRADPARNTSGSYGLGLSIAEKTVQLHHGKIHAESRDGI
                     NRFWVELPCVLGPGVQGYGFRL"
     gene            complement(2213..2893)
                     /locus_tag="ACZPCG_04070"
     CDS             complement(2213..2893)
                     /locus_tag="ACZPCG_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009447308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1284285.1"
                     /translation="MKILIIEDERLLAGSIKDLLEIQGFEAEAVYDGETGFTYAGTGI
                     YDLIILDVMMPGKSGYEVARELRASRLGTPILMLTARSALEDRIAGLDAGADYYLTKP
                     FDARELTACVRALLRRQGGQVDEVGMGKTCLDLSTAVLSYGDRQIRLSAKEFDIMRLL
                     FQSGAGNLSKEMILEKVWGYDSDAVENHVEVYIGFLRKKLLSIGSDVRIESIRRMGYH
                     LEVAEHDL"
     gene            complement(3025..5139)
                     /locus_tag="ACZPCG_04075"
     CDS             complement(3025..5139)
                     /locus_tag="ACZPCG_04075"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III family protein"
                     /protein_id="HIR1284286.1"
                     /translation="MSKVISVTEMFGKDVFNDAVMRDRLPKSVYKKLKKTIEDGAELD
                     PSIADVVAHAMKDWAIDHGATHFTHWFQPLTGVTAEKHDSFVSAPVEGRVIMEFSGKE
                     LIKGEPDASSFPSGGLRATFEARGYTVWDCTSPAFLKEDAIGVTLCIPTSFCSYKGEA
                     LDKKTPLLRSMQAINEQSLRILRLFGNTTAKRVNPSVGPEQEYFLVDRQKYLQRKDLI
                     YTGRTLFGAMPPKGQEMDDHYFGIIRERIGAYMNDVNKELWKLGVPAKTQHNEAAPAQ
                     HELAPIYEGVNQAVDHNQLVMETLKKVAGRHGMACLLHEKPFYGVNGSGKHNNWSLTA
                     DDGTNLMNPGDTPHENIQFLLVLACIIKAVDRHADLLRESASDVGNDHRLGADEAPPA
                     IISIFLGEQLEDVIDQLCDTGEATHSKKGGTLRTGVNILPDLDKDATDRNRTSPFAFT
                     GNKFEFRMVGSSDSVASANTVLNTIVAEAFKEAADQLEQAEDFDMAVHDLIKELLAAH
                     RRVIFNGNGYSEEWVKEAEQRGLPNLRSMVDAIPALVTDKAVKLFEEFGVFTRAELES
                     RAEVEYESYAKSINIEAKTMIDMAGKQIIPAVIRYTTQLAGSISAVRNACPEADVSTQ
                     TELLLESSDLLAETKTALSALEDATARCAAMESGKDQALAYHSEVVPAMEALRTPVDR
                     LEMLVDKELWPMPSYGDLIFEV"
     gene            complement(5346..6215)
                     /gene="fba"
                     /locus_tag="ACZPCG_04080"
     CDS             complement(5346..6215)
                     /gene="fba"
                     /locus_tag="ACZPCG_04080"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009245138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0030388 - fructose 1,6-bisphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HIR1284287.1"
                     /translation="MLVSAKDMLEKARAGKYAVGHFNINNLEWAKAVLLTAEELQSPV
                     ILGVSEGAGKYMTGFKTVAAMVSAMLEELNITVPVALHLDHGSYEGCYKCIKAGFSSI
                     MFDGSHYPIEENVEKTKELVKIAHTMGLSLEAEVGSIGGEEDGVVGAGECADPAECKA
                     IADLGVDFLAAGIGNIHGKYPENWQGLNFDVLEKVKEATGDMPLVLHGGTGIPEDMIK
                     KAISLGVAKINVNTECQLSFAEATRKYIEAGKDQQGKGFDPRKLLAPGAEAIRATVKE
                     KMELFGSVGKANA"
     gene            complement(6361..7857)
                     /gene="thrC"
                     /locus_tag="ACZPCG_04085"
     CDS             complement(6361..7857)
                     /gene="thrC"
                     /locus_tag="ACZPCG_04085"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002593966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR1284288.1"
                     /translation="MALSYQSTRGGQAGVTASQAVLQGLAADGGLFMPTEIPKLDVSI
                     EKLAGMTYQETAYEVMKLFLTDYTEEELKSCIAKAYDSKFDTEEIAPLAKADGAYYLE
                     LYHGSTIAFKDMALSILPHLMTTAAKKNHADKEIVILTATSGDTGKAAMAGFADVPGT
                     RIIVFYPKGGVSPVQERQMRTQKGENTAVVAIHGNFDDAQTGVKNLFNNKELAKELAE
                     KGFQFSSANSINIGRLVPQVVYYVYAYAKLLANEEIAAGEEINVVVPTGNFGNILAAY
                     FAKKMGLPIRTLICASNDNKVLYDFFTTGTYDRRREFILTNSPSMDILISSNLERLIY
                     LSTGSDPKANAALMKELAEDGHYTITDEMRAFMADFKGGFATEEENEAAIRTIFQDTG
                     YLIDTHTGVAAAVYGQYKNETGDQTKTVIASTASPFKFSRSVMDAVKGKAATEGKDEF
                     DVADELAAAAGLVLPKAVEEIRTAPIRHDRECAVDEMEETVKNILGIS"
     gene            8117..8884
                     /gene="sfsA"
                     /locus_tag="ACZPCG_04090"
     CDS             8117..8884
                     /gene="sfsA"
                     /locus_tag="ACZPCG_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HIR1284289.1"
                     /translation="MRYEHTVKGRFLERPNRFIAYVEVNGQRVICHVKNTGRCRELLT
                     SGCTVVLEFHPHAREMGRKTEFDLIAVYKGNLLINMDSQAPNRAALEWMENLSQKEPN
                     DNALRMLQPFDPSGSIWLPRKIRREVVHKDSRFDLAFMLENQQTGALKPAFMEIKGVT
                     LEENGIVRFPDAPTERGVKHLQGLSEAVAEGFEAFVLFVIQMEGVREFRPNDETHKAF
                     GDALRAGARAGLHILAYDCEVEEASMKIRREVKISLS"
     gene            8934..9167
                     /locus_tag="ACZPCG_04095"
     CDS             8934..9167
                     /locus_tag="ACZPCG_04095"
                     /inference="COORDINATES: protein motif:HMM:NF015956.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HIR1284290.1"
                     /translation="MVFPLCDLKAGETAQVAWIISEPAMERRLSRLGFRFGEPVTCIF
                     KSKTAGLSAYRLHKRVVGIRQRTTREILVRKNT"
     gene            9187..10500
                     /locus_tag="ACZPCG_04100"
     CDS             9187..10500
                     /locus_tag="ACZPCG_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1284291.1"
                     /translation="MKDLTKGTPARIMISFAVPVALGNMFQLCYSLADTRVVGSALGE
                     TALAAVGATASISTLFIGFLSGLTNGFSLLIAREFGAGNKEGVKRFAAGSLLLGAVTA
                     LSLTLACLAGLPWILGLLNVPQELLIQSDSYIRVILSGLFVTMLYNACASVLRAVGDT
                     TAPLLFLIFAAVLNVVLDLVFIMYFGWGIEGAAFATVLSQGVAACLSLIYMFCRYRIF
                     RFCPADFKISGEQVKKLYTSGLSMAAMMSLVFFGTLSLQCAINTFGQDIIVAHTAARK
                     ITEFCMLPFSVMGVTMANYCGQNTGAGRMDRIRTGIRQSLLLTWIWAVGMILLSWTIS
                     PRLASMVTGSQNETVLNTAALYLKVNTLFYFAPAAISILRNSLQGMGDHVTPICSSMI
                     ELAGKIIAAFLLADIFKYWGIIVAEPVVWVLMVIPLIVKTRALLR"
     gene            complement(10453..12444)
                     /locus_tag="ACZPCG_04105"
     CDS             complement(10453..12444)
                     /locus_tag="ACZPCG_04105"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002593960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUV3 family DEAD/DEAH box RNA helicase"
                     /protein_id="HIR1284292.1"
                     /translation="MEIRNECAVCLADCYKELFFGPSNRRCRAMTGAELRNRLSRLND
                     ACLEKIIKPNELSDIHAMLAKVCAFLMHKEGRIKPGPQLTRWEENCRCMEKFCEHLAK
                     KDLEFLPELTQEELEHVLKMQGIRRYLLTNSLERAYQLFYIPKTIKKGIRESVKRRPE
                     DEYPETRAMKRRFILHIGPTNSGKTHDALERLKQAEHGAYFGPLRLLALEVYDRLNSE
                     HVPCSMITGEETLEVPGAVCQACTVEMLNDHEYFDVAVVDECQMAADPYRGHNWTRAV
                     LGLRAEEIHLCMAPEAEDIMVQMIKRCGDSFQIVRHKRSTRLTLEKKPYSLKRDLKKG
                     DALIVFSKKSVLALAAHMENQGVRCSVIYGSLPPATRREQVRRFLERETEVVVSTDAI
                     GMGLNLPIRRIVFVETRKFDGTARRSLLPEEIRQIAGRAGRFGLYDEGFVTAIDDIET
                     IEDGLSARPLPIMKAYVGFPEQLLNLPAEIDTLVKIWAGMEAPAIYEKMEVDELLSLY
                     MSFERIHGDHLEEFTKQEIYKLITCSVDIDNRLVMDLWKDYCREYREVDELEFPYSPG
                     TDLYDMESYYKMLDLYFQFSRKTGLPVQEENLFHERRSTEEEISRILRTECGSYTRKC
                     SICGRELSWDHPFSICERCFERGRSSQKRSRFHDQRYNH"
     gene            complement(12468..12863)
                     /locus_tag="ACZPCG_04110"
     CDS             complement(12468..12863)
                     /locus_tag="ACZPCG_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6483 family protein"
                     /protein_id="HIR1284293.1"
                     /translation="MAYQDDYVMRTITDLVRAIAGLALGKNEIDYDLPLSEAAYTPVD
                     RAYRRLKDLADAGDINGAENELYECLDTSDRAYLEMALAFYMYLNQLDDDALYTANFS
                     REEIAEGINSVSAQFGISGFENFVDTTMV"
     gene            13060..14151
                     /locus_tag="ACZPCG_04115"
     CDS             13060..14151
                     /locus_tag="ACZPCG_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HIR1284294.1"
                     /translation="MKLNDRNTTPFSFIKKTDKKMTSSLILAVLSVLLLGCARTFPVF
                     AQWYSVTIYAGLKNTIARFSGLFPFSLSEAGLYLLLAFCLYWILHSLKRPLLLFKNAF
                     FLASLLFFIYTINCGINYYRKPFSEEAGFSEELKKGSTAAELYSLCEYLVKQVNETVP
                     WENSPKRNASFFRSMRELGQEAMANLGADFPQLGGWYPYTKPLLNPRLLSVQQLTGIY
                     SPFTIEANYNSEMPFYNIPHTICHELSHLKGYMREDEANFIGYLACIGSDAEAFQYSG
                     YLTGWVYAGNALAKADFEGYCRLYEQLDPQAIEDLGENNRFWDQFEGTVAEVSTKVND
                     TYLKAHSQTDGVKSYGRMVDLMLVYYRSF"
     gene            complement(14123..15334)
                     /locus_tag="ACZPCG_04120"
     CDS             complement(14123..15334)
                     /locus_tag="ACZPCG_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004849666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIR1284295.1"
                     /translation="MLTSLSFIFLLGLAMAAVCQRLKLPRIIGMLLTGILLGPYALNL
                     LDPSILAVSADLRHMALIIILLKAGLSLNLADLKRVGRPAVMMACVPASFEILGFLIF
                     APHILGVTRVEAAVMGAVLSAVSPAVVIPRMVQLMEERYGTDKSIPQMIMAGASCDDI
                     FVIVLFSTFVTMAQGGAVHGTDLLNVPVSIILGVALGVGTGYALSLFFEKAYVCGRYV
                     RNSTKVIILLGVSFLLMAVETWLKEIVSISGLLAVVSTACVLKIRSIPDVSSRLSEKF
                     GKLWIAAEVILFVLVGAAVDIRYTLEAGLPALAMIAAALVFRSLGTMFCLFGTALNLK
                     ERLFCIIAYLPKATVQAAIGSVPLALGLPCGALVLSVAVLAIVITAPLGALGMDLTYK
                     KFLKKNGSIPA"
     gene            complement(15372..16583)
                     /locus_tag="ACZPCG_04125"
     CDS             complement(15372..16583)
                     /locus_tag="ACZPCG_04125"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016285653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HIR1284296.1"
                     /translation="MKYDFTSIMERHGKDAIAVDGLGTMPGFAPDPPKEGFDIIPMWV
                     ADMNFPTVPTVQEAMAERIGHPAFGYFSPSDDYFNAIIRWQERRNGVRDLEKKHIGYE
                     NGVLGGLLSALNVFCSKGDHVLLNSPTYIGFTKSLANNGYRAVHSPLYLDEENVWRMD
                     YEDMEKKIVEYKIHAAVFCSPYNPVGRVWTRDEIERAMEIFKRHNVFVVSDEIWSDII
                     TLGHKHIPTQSVSEDARQRTVALYAPSKTFNLAGLVGSYHIIYNDWIRDRVEKESSLS
                     HYNEMNVLSMHALIGAYRPEGYEWVDELCATITGNIDYAADYIETHFEGVKVSRPEGT
                     YMLFIDVEEWCRKHGKTLEEVEKAGWDVGVAWQDGKMFFGPWSIRMNLALPLSRVKEA
                     FERLRRYVFQG"
     gene            complement(16580..17446)
                     /locus_tag="ACZPCG_04130"
     CDS             complement(16580..17446)
                     /locus_tag="ACZPCG_04130"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013213.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HIR1284297.1"
                     /translation="MKIGENSTAEQYVAEAGRFWEEGDGLIDAHAHIGTEEEIRERIR
                     DGIPSVICAGSPKETEQLKRLFNRFEVHPVIIPAAGLHPWNSDKYRAEEMFPFMEEWP
                     VMGEIGLDSLWCDVPLNVQKEAFECQLDFAVKKRKPVVLHTKEQEAEIAGILRRYPNR
                     YLVHWYSCGQHLEEYLDQDCYVSIGPDVVWNPAVQEAARQVPEDRLLIETDGLGAVKW
                     AWEEGEKHGIMESQFHTALPELTVRSSLEQTLREVSRLRGVEMNVLAKAIKRNFRRFV
                     WGDNTGQKEENR"
     gene            complement(17443..18177)
                     /locus_tag="ACZPCG_04135"
     CDS             complement(17443..18177)
                     /locus_tag="ACZPCG_04135"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1284298.1"
                     /translation="MKLEISGLTFSYENKKIFDGLEFSIREGEFVSLLGPSGCGKSTL
                     LKLLTGVIAPEKGHILTDGQEIRGITEHFAYMPQNDLLFPWKTILENVCLYGRIHGGQ
                     EQVKKEALSQMGAFGLEGYENEYPSALSGGMRQRAAFLRTLLCRADILLLDEPFGALD
                     VITRGEMQDWLLGVRARLNRTVLLVTHDMDEAIYLSDRILILDPGRGRIGSEIEIAEK
                     NRSRDWLYSQGELRRRIYREIMGDRT"
     gene            complement(18247..19266)
                     /locus_tag="ACZPCG_04140"
     CDS             complement(18247..19266)
                     /locus_tag="ACZPCG_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1284299.1"
                     /translation="MGNYGIFVRKIAVGILAAAVAAGLSGCADTASEKAAEGESPENV
                     TVILDYVANTNHTGMYVALDQGYYKEDGMDVSIVEPTEGATATLIAVGKGDFGISYQE
                     DVTIARTSADPLPVKAVAALIQHNTSGFVTYGDKDIHSPKDFEGKTYAGWGGPGEEAV
                     LKAVMAKDGGDFSKLDMVISDGSGFEALKDKVDVMWFFEGWDNIKCRLNDFPINYMEL
                     RQLDKRLDYYTPVIIASEDTLKNKPEMTKKFLEATAKGYRYAIEHPKESAEILHKYAP
                     DYSLEMLTMSQEYLAGKYMEDTKRWGEMKDSVWDNYTDFLVEYGVIEEDIPADQCYTN
                     EFLPQ"
     gene            complement(19270..20028)
                     /locus_tag="ACZPCG_04145"
     CDS             complement(19270..20028)
                     /locus_tag="ACZPCG_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004462115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1284300.1"
                     /translation="MKQVSSRMAPAGYILVILLLWELAVRLFHIPLYILPAPTAIAAA
                     LGEEWPVLVSHGAVTVLEALAGILISLILALALGILMDRFPAVQRGIYPLLVVTQTVP
                     MIVLAPILIIYMGFGPGPKILTVVLMCFFPVAVSFSDGLSRVDEEYVHLVRSYGAGKW
                     GAYRLVKIPAALPALLSGLKVAATYSISGAVVGEWIGSQQGLGYYLLRVKNGYMLDKV
                     FACVAVIIGLSLCVNGAVRLLGRICLPYERKRRK"
     regulatory      complement(20092..20195)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(20192..>21337)
                     /locus_tag="ACZPCG_04150"
     CDS             complement(20192..>21337)
                     /locus_tag="ACZPCG_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002593953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR1284301.1"
                     /translation="DGKVVEYLPELLEAFMDQVIEEHPSALVLSGDITMNGEKINHEE
                     LARGLMRVQDAGIPVLIVPGNHDINNPHAAVYFGEEKAETDPVTPEEFYNIYHMYGYD
                     QAISRDDASLSYVYQLDERNRLLMLDTCQYEPKNMVEGQLKVETLRWADEQLKKAKED
                     GMNVLPIGHHNLLAQSRMYTTQCAMNNNGDVIELFQKYELPLYLSGHLHVQRIRKHKA
                     EPGAADDSYGIMEIVTDALSIPPCQYALLEWKRDGSMEYSTKAVDVSAWAARTGSQNP
                     DLLDFEGWSERYIQKLIIDQIRGVVKNLGNDVEHSMAEVYASVYIDYYAGRRIDAKGI
                     RSSKGYRWWERNMPDSYLLKELDAMIDDSDRDNNYMLLPEVNAAAEE"
BASE COUNT         5249 a         5858 c         4733 g         5497 t
ORIGIN      
        1 ataaaaagaa ggatcaaaat ggtggacgac agggtcgtga gcccgtcaat ggcgtaagca
       61 aggcccagat ggttctcaaa caggattcct cctatggttg gggacagaat gatccccaga
      121 ttgagtccca gatacataag gctgtaggct ttttccctgt tttcggaggt gctcagatcg
      181 gctaccagtg cgtcatagga cggattttcc attgtctgaa agattcctgc tgtaaaaaac
      241 agcaggatct gtgccatgga tacaggaaga accgctgctg tcaggtaaca gatgaccgtt
      301 accaaatcgc agataatgat gagctttttc ttattgcagc agtctgccag ctttcctccc
      361 agcaggtttg caggaaactg taccactccc atagctatgg tgatcttggc gatctcctgt
      421 gcattcattc ccagcttatt gcttaaaatc agcgtcagca tgggccatat catagctccc
      481 atatttgttg ctgcctttcc ccagaaaaga atatataatt ctcttctcat acctctgtac
      541 tggccgaaca ttttctcaaa tattgttttc atttctaatt atcctcttaa aatatatgct
      601 gctatatgct tttccacctg tgtcagaccg cactttcctg tgaaataaaa aaacagagac
      661 cctctgctgc caaaggatct ctgcatagta tcttctcttt tagatagggc cgggcccagc
      721 agccagccgc ccattgaaag atgggcggct ggcgtgtgcg gtataatata cggcataaaa
      781 agagagaacc tgatatccga tccattttgg gcaacatgaa ataacaggac gctttcacgg
      841 tattgcgcct gcacagacaa atcttcatgc gtcaaaataa atggatcaaa ttaacatctg
      901 gcctctcctt taaaaatctg aagtatggct tcagtatagc actggaaata tatggatgca
      961 agagggaatc atccgacttt atagccggaa accgtagccc tgcacaccag gccccagaac
     1021 acagggcagc tccacccaaa atcgattgat cccatccctg ctttcagcgt gtatttttcc
     1081 gtgatgaagc tgtacggtct tttccgcaat ggaaaggccc aggccgtagc ttccggaggt
     1141 attgcgggcg ggatcggcgc ggtaaaagcg tttgaagatc tggcttaagt cttcgggatc
     1201 aatcgaagga ccctgatttt ctacagtgag tatgcagtgg ctttttccct gttttttaag
     1261 ctttacccag gtggttccgc cctggctcga atatttgcag gcattgtcta aaagaatatc
     1321 cagaagctgg ctcagcctgt cctttaagcc ccagatcaga ataccttctt ctatttctgc
     1381 ataaagcgtt aatccctgtt caaaataaag gggttcaaaa agaagaagcg aatcctctgc
     1441 tgtccggctt aaatccacct gttcaaaagc tgcttctgcc tgtccgctgt cagcacgggc
     1501 cagatccagc atctgttcga tcagccccct catctgccgt gacatggcaa ggatggcttc
     1561 ggaaaaacgt ctctgctctt cttccccaca gccggaggac tgcagaagct ccgcattggt
     1621 cataattaca gtcaaaggcg tttttaactc gtgggaggcg tcggccacaa actggcgctg
     1681 ctgtttccag gccttttcca ccggccgcac cgcccagccg gaaagccaga tgctgatggc
     1741 taaaaatagg ataaaactca gacccgcgat cagggcgcag gttctcacca gcccctttag
     1801 cgccgccgtt tcactggaaa tatccgcaaa taccaggcta tacctgtagg gcgtattggt
     1861 cttatagtac cgaaagccgt attcctttat cactcctacc ttttgatggg aggaacgtgc
     1921 ccttacaatg agatccatga gaaattcctg atcagacaga tcaaaatagc cgccgctgct
     1981 ggcgatcagt tctcccccgg ttcccagatg caggataaaa aaaggaagac ggatctgctc
     2041 gctctgttct ccgggcatct caatctgaaa gggtcggttt gcaatctttt ccatcatgcg
     2101 gatgctcttt tcttccagat ctgctcttgt aaaatggtac accaggccga ataccacact
     2161 catcatcaga accactatcg tcatgttgat aactacaaac ttcactctca gcctataaat
     2221 catgttctgc cacctccaga tgatatccca ttcgccggat ggactcaata cgtacatctg
     2281 acccaatgct taacagcttt ttcctcaaaa aaccaatata aacctctaca tggttttcca
     2341 ctgcatctga atcatagccc cacacctttt ccaggatcat ttccttagaa agatttcctg
     2401 ccccggactg aaataaaagg cgcataatat caaactcttt cgcagaaagc cggatctgtc
     2461 tgtccccgta gcttaaaaca gccgtcgaaa gatccaaaca ggtctttccc atccctacct
     2521 catccacctg tcctccctgg cggcgaagca aggcgcggac gcaggcagta agctctctgg
     2581 cgtcaaaggg ctttgtcaga taataatctg ccccggcatc cagccctgcg atccgatcct
     2641 ccagagcaga acgggccgta agcattagaa tgggcgttcc cagacggctg gcccgaagct
     2701 cccttgccac ctcatagcca ctttttcccg gcatcatcac atccagtatg atcagatcat
     2761 aaatcccggt gcctgcatat gtaaaaccgg tttctccgtc atatacagcc tcggcctcaa
     2821 aaccctggat ctctaaaaga tcttttatag aaccggccag cagccgctca tcctctatga
     2881 tcagaatttt catatacatc cccttactta ctgataatat ccctattata cagcaaagcg
     2941 gccaaagaaa acagcctaca gctatcccct tccgggcagc cgcaggctct gtcttctaaa
     3001 atgaccagtt aaacaattgt cgtattacac ttcaaaaatc agatcgccgt agcttggcat
     3061 gggccacagt tccttgtcca ccagcatctc cagacggtct acaggagtcc ttaaggcctc
     3121 catggcagga acgacctcac tgtggtatgc cagtgcctga tcctttccgc tttccatggc
     3181 tgcgcagcga gccgtagcat cttccaaggc agacagggct gtttttgtct cagccagaag
     3241 gtctgaggat tccagcaaaa gctctgtctg tgtgctcacg tctgcctccg ggcaggcatt
     3301 tctcaccgcg gaaatggaac ctgccagctg ggtggtgtag cggatcactg caggaatgat
     3361 ctgctttcct gccatatcga tcatagtctt tgcctcaatg ttaatgctct ttgcatagct
     3421 ttcgtactct acctcggctc tggactccag ctcggctctg gtgaatacgc cgaactcctc
     3481 aaacagcttt accgccttgt ctgtcacaag agccgggatc gcgtctacca tagagcgaag
     3541 gttgggaagc cctctctgtt cggcttcctt tacccactcc tcagaatagc cgtttccatt
     3601 gaagataact cttctgtgag ctgccagaag ctctttaatg aggtcatgga ctgccatgtc
     3661 aaagtcctcg gcctgctcca gctgatcagc cgcttcctta aaggcttctg ccacaatggt
     3721 atttaacacc gtattggcag atgctacgga atcagaggaa cctaccatac ggaactcaaa
     3781 tttattgcct gtaaaagcaa agggtgaggt gcggttccgg tcggtagcat ccttgtccag
     3841 atccggcagg atatttacgc cggtgcgcag agttccgccc ttcttggaat gggtcgcttc
     3901 tccggtgtcg caaagctggt cgatcacatc ctccagctgc tcgcccagga aaatggaaat
     3961 aattgccggc ggagcctcgt ctgctcccag tctgtgatca tttcccacat cagaggcaga
     4021 ttcacggagc agatctgcat ggcggtctac agccttgatg atgcaggcta aaaccagaag
     4081 gaactggata ttctcatggg gcgtatcgcc ggggttcatc aggttggtgc cgtcgtcggc
     4141 agtcagggac cagttgttgt gcttaccgga gccatttacc ccatagaaag gcttttcgtg
     4201 gagaaggcag gccatgccgt ggcgtccggc taccttcttt aaggtctcca tcaccagctg
     4261 attgtggtct acagcctggt tcacgccctc atagatggga gccagctcat gctgggccgg
     4321 agctgcctcg ttgtgctggg tcttggcagg cactcccagc ttccacagtt ccttatttac
     4381 atcgttcata taggcgccaa tccgctctct gatgatgccg aaatagtggt cgtccatctc
     4441 ctgccccttt ggcggcatag cgccgaataa ggtgcggccg gtataaatta aatcctttct
     4501 ctgcagatac ttctggcggt caaccaggaa atactcctgc tctggtccca cggaaggatt
     4561 gacccgtttt gcagtggtgt ttccaaacaa gcgcaagatc cgcagggact gctcattgat
     4621 ggcctgcatg gagcgcagca gaggggtctt tttatccaga gcctcaccct tataggaaca
     4681 gaaggaggta ggaatacaga gggtcacgcc gatagcgtcc tcttttaaga aagccggaga
     4741 ggtacagtcc cataccgtat agcctctggc ctcaaaggtt gcgcgcaggc cgccggaggg
     4801 gaaggaggaa gcatccggtt caccctttat gagttctttt ccagaaaact ccatgatcac
     4861 acggccttca acgggagcgg aaacaaagga atcgtgcttc tcagccgtaa cgcctgtaag
     4921 aggctggaac cagtgagtaa aatgggttgc accatggtca atcgcccagt ctttcatagc
     4981 atgagctacc acatctgcaa tactgggatc cagctctgcg ccatcctcaa tggtcttttt
     5041 aagcttctta tatacgcttt taggcagacg gtctctcatc acggcatcat taaacacatc
     5101 cttgccaaac atctctgtaa cacttattac tttgctcata gattctccat cctctctttt
     5161 catggtgtcc cctttcccca atgcacccca aaaggcggtt ttgtacagca aaaggcgttc
     5221 tctccttttt caaaaagaga acgccctcgt tcttttgtta taaaataaac catttcgcgt
     5281 gaaaatgcaa gcggtttttc caaaaacaga atcagtttat tctaataagt tttaattcac
     5341 agagcttatg catttgcctt tccaacagaa ccaaacagct ccatcttctc ttttacagtt
     5401 gcgcggattg cctctgcgcc gggagctaac agctttcttg ggtcaaagcc cttgccctgc
     5461 tgatcctttc ctgcctcgat gtactttctg gtagcctctg cgaaggaaag ctggcactct
     5521 gtatttacgt tgatctttgc aacgcccagg ctgatagcct tcttgatcat atcctcggga
     5581 atacctgtac cgccgtgaag aactaacggc atatcaccgg ttgcttcctt aaccttctcc
     5641 agtacgtcga agtttaagcc ctgccagttc tccggatact taccgtggat atttccgata
     5701 cctgctgcaa ggaagtcaac acccagatca gcgatagcct tgcactctgc aggatctgca
     5761 cactcgcctg cgcctaccac gccgtcttcc tcgccgccga tagagcctac ctcagcctct
     5821 aaggacaggc ccattgtatg agcgatctta accagctctt ttgtcttctc cacattctct
     5881 tcgatcggat aatgagagcc gtcaaacata atagaggaga atccggcctt aatgcactta
     5941 tagcagccct cgtaggagcc gtggtctaag tgaagtgcca ccggtacggt aatatttaat
     6001 tcctcaagca tagcagaaac catagctgct acggtcttaa atccggtcat gtacttgcct
     6061 gcgccctcgg atacacccag gataaccggg gactgaagct cttctgcagt cagaagaact
     6121 gcttttgccc actccagatt attgatattg aagtgaccta ctgcatattt gcctgctctt
     6181 gctttttcaa gcatatcttt tgctgatact aacataatat ttcctcctta tacttgggcg
     6241 ctcccccagc tgcatgaacc cggaacccgg ataacagccc tggaacgcag ttctactttg
     6301 ttatgttttt aacagtttcg aattcattat aaaccatacc atccaaaaat ggaagaactt
     6361 ttatgaaatc ccaaggatat tcttaacggt ttcctccatc tcatccacag cgcactctct
     6421 gtcatggcgg atgggcgcag tacgtatctc ctctaccgct tttggaagca caaggccggc
     6481 agctgcagcc agctcatcgg ccacatcaaa ctcatccttt ccctctgtgg ccgctttacc
     6541 ctttacagca tccattacac tgcgtgagaa cttaaaggga ctggccgtgg acgcaataac
     6601 cgtctttgtc tgatcgcctg tttcattttt atactgtccg tatacggccg ctgccacgcc
     6661 tgtatgtgtg tcgatgagat agcctgtatc ctggaaaatg gtccgtatcg cagcttcatt
     6721 ttcctcctct gtggcaaaac cgcccttaaa atcagccata aaggcccgca tctcatccgt
     6781 aatggtataa tggccatcct ctgccagctc cttcatcaga gcggcgtttg ctttcggatc
     6841 cgagcctgta cttaaataga tcaggcgctc cagattgctg gagatcaaaa tatccatgga
     6901 cggagaattg gtaagaataa actcccttct gcggtcataa gtgcctgtgg tgaagaaatc
     6961 gtacagcacc ttattatcat tggaggcaca gatcagagta cggatgggaa gccccatttt
     7021 cttagcaaaa taggctgcga gaatgttgcc gaaattgcct gtgggaacca ccacattgat
     7081 ctcctccccg gcagcgatct cctcatttgc cagaagcttt gcataggcgt atacatagta
     7141 cacaacctga ggcaccagac ggccaatgtt aatggaattg gcggaggaaa actggaatcc
     7201 cttctctgcc agctcctttg ccagctcctt gttattgaaa agattcttta ctccggtctg
     7261 ggcgtcgtca aaattgccgt ggatggcgac aactgccgtg ttctccccct tctgggtgcg
     7321 catctggcgc tcctgcaccg gacttacgcc gcctttggga taaaatacga tgatacgggt
     7381 gccgggtaca tccgcaaagc ctgccatagc tgcctttccg gtatcgccgg aagtagccgt
     7441 taagatcacg atttccttat ctgcatggtt cttctttgcc gcagtggtca tcagatgagg
     7501 cagaatagat aatgccatat ccttaaacgc aatggtgctc ccatggtaaa gttccagata
     7561 ataagcgcca tcggcctttg ctaacggagc gatctcctct gtgtcaaatt tgctgtcata
     7621 ggctttcgca atacaggact tcaattcctc ttccgtataa tctgtgagga aaagcttcat
     7681 cacctcatat gcggtctcct gataggtcat tccggccagc ttttcaatag aaacatccag
     7741 cttcggaatt tcagtgggca taaacaggcc gccgtcggca gccagaccct gaaggacggc
     7801 ctgggatgct gtgaccccgg cctgtccgcc tctggtactc tggtaagata atgccataat
     7861 caatttcctt tctttatgcg atgtatactt tgagggactg ccagtctgaa cgcacagccc
     7921 cggaccctta tttgcagaaa gtttagcata ttttaatgag aggtgcaact gttaaggggc
     7981 aggaacggga atttttcttg aaattactgt tgtaaaagaa gaaaacggcc gcaggaaaca
     8041 cttgcgtgaa cccaaaattt gccttattat gatagccgaa acaaaaaccg gatttttccg
     8101 gaaaaatgag gttgacatga gatatgaaca tacagtgaag ggacgttttc tggaacgtcc
     8161 caatcgcttt atcgcctatg tggaggtaaa cggccagcgt gttatctgcc atgtaaaaaa
     8221 tacaggacgc tgcagagaac tgcttacctc cggctgtacc gtggtgctgg aatttcaccc
     8281 ccacgccagg gaaatgggaa ggaaaacaga atttgaccta atcgcggtct ataagggaaa
     8341 ccttctgatc aatatggatt cccaggcgcc caaccgggct gccctggaat ggatggagaa
     8401 cctctcccaa aaggagccaa acgacaacgc gctccggatg ctccagccct ttgatccatc
     8461 tgggagcatc tggctgcccc gtaagatccg gcgggaggta gtccacaaag attcccggtt
     8521 cgatctggca tttatgctgg aaaaccagca gaccggagcc ttaaaacctg ccttcatgga
     8581 gataaagggc gtaaccctgg aagaaaatgg aatcgtccgc tttcccgatg ctcccacaga
     8641 gcggggtgta aagcaccttc agggcctgtc cgaagctgtt gccgaaggct ttgaggcctt
     8701 tgtgctcttt gtcatacaga tggaaggagt aagggaattt cgtcccaatg atgaaacaca
     8761 caaagccttt ggggacgccc tgcgggcagg agccagagcc ggactgcata ttctggccta
     8821 tgactgtgag gtagaggaag cttccatgaa aatccgcaga gaggtaaaaa tcagtctctc
     8881 ttaagtcctg ttatcatata ctatggtatc agggtcaaaa ggatggataa aacatggtat
     8941 ttcccttatg cgatctgaaa gccggagaaa cagcccaggt agcctggatc atatccgagc
     9001 ctgccatgga gcgccgtctg agccgcctgg gctttcgctt cggagagccg gtaacctgta
     9061 tttttaaaag caaaacggca ggcttgtcgg catatcgtct tcataaacgg gtcgttggaa
     9121 tccgccagcg aaccacccgt gaaattctgg tacggaaaaa cacttaaaac acgaaaggat
     9181 ttacatatga aggatcttac aaaaggcaca ccggcccgga tcatgatcag ctttgctgtt
     9241 cccgtagccc ttggaaatat gtttcagctg tgctacagtc tggctgatac ccgcgtagta
     9301 ggaagcgccc tgggagaaac tgctctggca gctgtggggg ccaccgcctc catcagcact
     9361 ctttttatcg gctttctgtc cggccttaca aacggctttt ctctgctaat cgccagagaa
     9421 ttcggcgcag gaaacaaaga aggagtaaaa cgctttgccg caggcagcct gctccttgga
     9481 gctgtgactg ccctgtccct cacactggcc tgtctcgcag gcctcccatg gatcctcgga
     9541 cttttaaacg tgccccagga gcttcttatc caatcagaca gctatatccg cgtcattctg
     9601 tccggactgt ttgtcaccat gctgtacaat gcctgcgcct ctgtcctgcg ggctgtaggc
     9661 gatacaacag cccctctgct gtttctgatt tttgcagctg tattaaatgt ggttttagat
     9721 ctggttttta tcatgtactt tggctggggc atagaaggag ccgcctttgc tacggttctg
     9781 tcccaggggg tggcggcctg cttaagcctg atctatatgt tctgcagata ccggatcttc
     9841 cgcttctgcc cggctgattt taaaatatcc ggagaacagg tcaaaaaact ctacacctcc
     9901 ggcctctcta tggctgccat gatgtccctt gtatttttcg gcaccctgtc gctccagtgc
     9961 gccatcaata cctttggcca ggatatcatc gtagcccata cagccgcccg gaaaattaca
    10021 gaattttgta tgcttccctt cagcgtcatg ggcgtaacca tggcaaatta ctgcggacag
    10081 aatacgggag ccggccgcat ggaccgcatc cggacgggaa tccgccaatc ccttcttctg
    10141 acctggatct gggccgtagg aatgatcctt ttaagctgga ccatttctcc ccggctggct
    10201 tctatggtta caggctccca aaacgagacg gttctcaata ccgcagctct gtatctgaag
    10261 gtcaacaccc tgttttattt tgctccggct gctatttcta tcttgagaaa cagccttcag
    10321 ggcatgggcg atcatgtgac tcccatatgc tccagcatga tcgaactggc tggaaaaatc
    10381 attgccgcct tccttctggc ggatattttt aagtactggg gaatcatcgt ggcagagcct
    10441 gttgtatggg ttttaatggt tataccgctg atcgtgaaaa cgagagcgct tctgcgatga
    10501 acggccccgc tcaaagcagc gctcacagat ggaaaatgga tgatcccagg aaagctctct
    10561 gccgcagatg gaacatttcc gggtatagct gccgcattct gtgcgcagga tacggctgat
    10621 ctcctcctct gtgctccgcc tttcatggaa caggttttct tcctggacag gaagtcctgt
    10681 cttccgtgaa aactgaaaat aaagatccag cattttataa tagctttcca tatcgtaaag
    10741 atccgttccc ggactgtagg gaaattctag ttcatccact tctctgtatt cccggcagta
    10801 atccttccac agatccatca ccagacggtt atcaatgtct accgagcagg taataagctt
    10861 atagatctcc tgtttggtaa attcttccag atgatctccg tggatccgct caaagctcat
    10921 gtagagagat aacagctcat ccacctccat tttttcataa atggcaggcg cctccatacc
    10981 ggcccagatc tttaccaaag tgtcaatctc tgcaggcagg ttcaaaagct gctcaggaaa
    11041 acccacatag gctttcataa tgggaagagg gcgggcggat aagccgtcct caatggtttc
    11101 tatatcgtca atggccgtca caaagccctc gtcatagagg ccaaagcggc ccgcccgtcc
    11161 tgcgatctga cggatttcct ccggcagaag actgcgtctg gccgtgccgt caaattttct
    11221 tgtttccaca aacacgatcc ggcggatggg aagatttaat cccatcccaa tggcgtctgt
    11281 gcttaccacc acctccgtct cacgctccag aaaacggcgc acctgctccc tccgggtagc
    11341 aggcggcagg cttccgtaga tcacactgca gcgcaccccc tgattttcca tatgggcggc
    11401 cagagccaga acggacttct ttgaaaatac gatcagagcg tctccctttt tcagatcccg
    11461 cttcagggag tagggcttct tctcaagggt aaggcgcgta cttcttttgt ggcgcacgat
    11521 ctgaaaggaa tccccgcagc gcttgatcat ctgcaccata atatcttctg cctcaggagc
    11581 catgcacagg tggatctcct ccgcacgcag ccccagcaca gcacgggtcc agttatggcc
    11641 ccggtaagga tctgctgcca tctgacattc atctaccacg gctacgtcaa agtattcgtg
    11701 atcatttaac atttccaccg tacaggcctg acagaccgct ccgggaacct ccagcgtctc
    11761 ctctccggta atcatggagc agggtacatg ttcactgttt aaacggtcgt aaacctccag
    11821 cgccaaaagg cgaagggggc cgaaataggc tccgtgctct gcctgtttta accgctccag
    11881 tgcgtcatgt gtttttccgc tgttagtagg gccgatatga aggataaaac ggcgcttcat
    11941 ggcccgtgtc tccgggtatt catcctccgg acggcgcttg accgactccc tgatcccctt
    12001 tttaatggtt ttggggatat agaaaagctg ataagcccgt tccagtgaat tcgttagaag
    12061 atatctgcgg atcccctgca tttttaatac gtgctccagc tcctcctgcg taagttccgg
    12121 cagaaattcc agatcctttt ttgccagatg ctcacagaat ttctccatac accggcaatt
    12181 ctcctcccac cgggtaagct gaggaccggg ctttatgcgt ccctctttat gcatcaggaa
    12241 cgcacagact tttgccagca tggcgtgtat atcgcttaat tcgtttggtt ttataatttt
    12301 ttccagacac gcatcattta accgggacag acgatttcgc agctctgccc ctgtcatggc
    12361 ccggcagcgt ctgttggacg gaccaaaaaa cagttcctta tagcagtcgg ccagacagac
    12421 ggcgcattca tttcttattt ccattaaccg ctctctttca gacatgctta taccatagta
    12481 gtgtctacga aattctcaaa accggagatg ccaaactgag cgctgacact gttaatccct
    12541 tctgcaattt cctctctgga aaaattggcg gtataaagag catcatcatc cagctggttt
    12601 aaatacatat aaaatgccag agccatttcc aaatacgccc tgtcactggt atccagacat
    12661 tcatacagct cattctcagc cccgttaatg tctcctgcat ctgcaagatc ctttaaacgg
    12721 cggtaagccc gatccacggg cgtgtaagcc gcctctgaga gaggaaggtc ataatcaatt
    12781 tcatttttcc ccagtgccag tccggcaatg gcccgaacaa ggtcggtaat cgtacgcatt
    12841 acataatcat cctgatatgc catagttgtt cactcctgat tgtttgctgc ccattccagt
    12901 cccagagagc agacctttgg ggacttattt tctactaagt ataggggata ccgccagctg
    12961 agtcaagatg cttcattccc cctttgcctt tccctcccct tgactcccat tttcccagca
    13021 attataatgg aaaaaatacg aaagagaaca aatgtgtata tgaaactaaa tgataggaat
    13081 acaaccccat tttcctttat aaaaaaaacg gacaaaaaaa tgacatcaag tctcattctg
    13141 gccgttttat ctgttctgct cctgggctgt gccagaacat ttcctgtatt tgcccagtgg
    13201 tactccgtaa ccatttatgc cggtcttaaa aataccatcg cacggttttc cggccttttt
    13261 ccattttcac tgtctgaggc aggactttac cttcttctgg ctttctgcct gtattggata
    13321 ctccacagct taaaacgccc tctcctgctt tttaaaaatg ccttttttct tgcctcgctt
    13381 ttatttttta tctatacaat aaactgcgga atcaattatt acagaaagcc cttttccgaa
    13441 gaggccggat tttctgaaga attaaaaaaa ggcagtacgg ctgcggagct ttattcgctc
    13501 tgcgaatatc tggtaaagca ggtaaatgaa actgtgccat gggaaaactc tcccaaacgc
    13561 aatgcttcct ttttccgttc tatgagggaa ttgggacagg aggctatggc aaatttgggg
    13621 gctgattttc cccagcttgg gggctggtat ccttacacca aaccgttact gaatccccgg
    13681 cttctatctg tacaacagct tacaggaatc tactctccct ttaccattga agcaaattac
    13741 aacagcgaga tgccatttta caatattccc catacgatct gccatgaact ttctcatcta
    13801 aaagggtata tgagagaaga tgaggcaaat tttatcggat atctggcctg tatcggatcc
    13861 gatgcagagg cgtttcaata cagcggatat ctgaccggat gggtttatgc gggaaatgcc
    13921 ctggcaaaag cggattttga gggctactgc cgcctgtatg aacaattaga tcctcaagca
    13981 atagaggact taggagaaaa caaccggttc tgggaccagt ttgaaggaac ggtggcagag
    14041 gtatccacga aagtaaatga cacctattta aaagcccaca gccagaccga cggggtcaaa
    14101 agctatgggc ggatggtgga tcttatgctg gtatactacc gttctttttg agaaacttct
    14161 tataggtaag atccattccc aatgctccca gaggagctgt gataacaatc gccagaactg
    14221 ctacggacag caccagtgct ccgcagggaa gccccagggc caggggcacg gaaccgatgg
    14281 cagcctgaac cgtggcctta ggcagataag cgatgatgca gaacaggcgc tccttcaaat
    14341 tcaaagcggt tccgaacagg cagaacatgg tgcccagaga acggaacaca agggctgcag
    14401 caatcatggc cagtgccgga agtccggctt caagcgtata gcggatatct acagcggctc
    14461 caaccagaac aaaaagaata acctctgccg cgatccacag ttttccaaat ttttccgaaa
    14521 gacgggatga tacatctgga atgctcctta ttttcagcac gcaagctgta cttactacag
    14581 ccagaagacc ggatatggat acgatttcct tcagccatgt ttccactgcc atcagcagaa
    14641 aggatactcc cagaagaata ataacctttg tgctgtttct cacataacgg ccgcagacat
    14701 aggctttttc aaaaaacagg cttaaggcgt acccggttcc cacacccaaa gcaaccccaa
    14761 ggataatcga aacaggcaca ttcagcagat ccgttccatg aaccgctccg ccctgggcca
    14821 tggtgacaaa ggtggagaaa agaacaatga cgaaaatatc atcacaggat gctcctgcca
    14881 tgatcatctg agggatgctt ttatctgtac cgtacctctc ctccatgagc tgaaccatcc
    14941 ttgggattac caccgcaggc gaaacagcgc tcagtacagc gcccatgaca gcggcttcta
    15001 cccttgtaac tcccaaaata tggggtgcaa aaataagaaa tcccaaaatc tcaaagctgg
    15061 cgggaacgca ggccatcata acggccggac ggcccacccg ttttaaatct gccaaattaa
    15121 gcgacaatcc ggcctttagc aaaataatga tcagagccat gtggcgcagg tctgccgata
    15181 cagccaatat ggaaggatcc agcagattca gcgcataagg ccccaaaagg atgcccgtta
    15241 agagcatacc gatgatgcgg ggcagcttaa gccgctgaca cacagccgcc atagccagcc
    15301 ccagcagaaa aataaatgac agggatgtga gcatgttggt ttctccttaa taaatatgta
    15361 ccgtcccgga cttacccctg aaatacatac cttctcagcc gttcaaaggc ctcctttaca
    15421 cggcttaagg gaagggccag gttcatgcgg atgctccagg gaccgaaaaa catctttccg
    15481 tcctgccatg ccacgcccac atcccagccg gctttttcca cttcctctag tgtctttcca
    15541 tgtttcctgc accattcctc cacatcaata aacagcatat aggtgccttc cgggcgtgaa
    15601 acctttactc cttcaaaatg agtttcgata taatctgccg cataatcaat atttcccgta
    15661 atggtcgcgc acagctcgtc cacccactca tatccctccg gcctgtaagc gccgatcagg
    15721 gcgtgcatgg acagcacatt catctcattg tagtgggaca gggaggactc cttctctacc
    15781 cggtcgcgga tccagtcatt gtaaataata tggtagcttc cgaccaggcc tgccagatta
    15841 aaagtcttgc tgggcgcata gagagccacg gtccgctggc gggcatcctc gctgacgctc
    15901 tgtgtgggga tatgcttatg gcccagagtg atgatatccg accagatttc atctgataca
    15961 acaaatacat tgtgcctctt aaaaatctcc atagcacgtt cgatctcatc ccgtgtccac
    16021 acccgtccga ccggattata gggggaacag aaaacagctg catggatctt atattccaca
    16081 atctttttct ccatgtcctc ataatccatg cgccatacat tttcctcatc cagatacagg
    16141 ggagagtgga cagcgcggta tccgttattg gcaagagatt tggtaaagcc gatataggta
    16201 ggagaattta aaagcacatg atcccctttg gagcaaaata catttaaggc gctgagaagg
    16261 ccgcccagaa caccgttttc atatccaata tgcttctttt ccagatccct taccccgttt
    16321 ctcctttcct gccaccggat aattgcatta aaataatcat cggacggaga aaagtatcca
    16381 aacgccggat gtccgatgcg ttcagccata gcctcctgga ctgtaggtac agtaggaaaa
    16441 ttcatatccg ctacccacat ggggatgata tcaaagcctt cttttggagg atccggtgcg
    16501 aaccccggca ttgtgccaag tccgtcaact gcaatggcat cttttccatg acgttccata
    16561 atgcttgtaa aatcgtactt catctgttct cctccttttg tccggtattg tctccccata
    16621 caaatctgcg gaaattccgc ttaattgctt ttgcaagcac attcatctcc actccccgga
    16681 gccgggatac ttcccgtaag gtctgctcca gagaactgcg gactgtaagt tcaggcagag
    16741 ctgtatggaa ctgtgactcc attataccat gcttttctcc ttcctcccag gcccatttca
    16801 cagccccaag accatctgtt tctatgagaa gccgatcctc aggcacctgt ctggctgcct
    16861 cctgaaccgc cggattccac accacatccg gcccgataga gacatagcag tcctgatcca
    16921 gatactcctc cagatgctgt ccgcaggaat accagtgaac cagataacgg ttaggatatc
    16981 ttcgcaggat tccggcaatc tcagcctcct gctctttggt gtgaagaact accggtttgc
    17041 gttttttcac agcaaaatcc agctggcatt caaacgcttc tttctgtacg ttaagaggta
    17101 cgtcacacca taacgagtcc agaccgattt cccccatgac cggccattct tccataaagg
    17161 gaaacatttc ctctgcccga tacttatcgc tgttccacgg atgaagccct gcggcgggaa
    17221 tgatgaccgg atgcacctca aaacgattga acagccgttt taattgctcc gtctcttttg
    17281 ggcttcccgc acatatcaca gaagggattc catcccggat gcgttcccgg atttcctcct
    17341 ctgtccctat atgagcgtgg gcatcgatca ggccatcccc ttcttcccaa aacctgcctg
    17401 cctctgccac atattgttct gctgtgctgt tttctcctat tttcacgttc tgtctcccat
    17461 aatctctctg taaatcctgc ggcgcagttc cccctgactg taaagccagt cccggctccg
    17521 gttcttttct gcaatctcta tctcgcttcc gatcctgcct ctgccgggat ctaagatcag
    17581 gatccgatcg gatagataga tggcctcgtc catatcgtga gtcaccagaa gaacggtgcg
    17641 gtttaatctg gctcggactc ccagaagcca gtcctgcatc tctcctctgg tgatcacatc
    17701 cagcgcccca aatggttcgt ccagaagcag gatatccgca cggcacaaaa gggttcttaa
    17761 aaatgcagct ctctggcgca ttcccccgga cagggcagag gggtattcat tttcatagcc
    17821 ttccagacca aaggctccca tctgtgacag ggcttctttt tttacctgct cctgccctcc
    17881 atggatcctg ccgtaaagac acacattttc caaaatggtt ttccatggaa ataaaaggtc
    17941 gttctggggc atatacgcaa aatgctccgt aatcccccgg atttcctgtc catctgtcag
    18001 gatatggcct ttttcaggag cgatcacccc ggtaagaagc tttaaaaggg tagatttgcc
    18061 gcagccggag ggccctaaaa ggcttacaaa ctctccttcc cggatggaaa attccagacc
    18121 gtcaaatatc tttttgtttt catagctgaa tgtaagaccg gaaatctcta atttcattgt
    18181 atctgcaggg aaatgctcct gcccctcctt tttatggtct gtcctgtttt atcatccgcc
    18241 gcatttttat tggggaagaa attcattggt ataacactga tctgccggga tatcctcttc
    18301 aattacgcca tattccacca gaaaatccgt gtaattatcc catacagaat ccttcatttc
    18361 gccccagcgt tttgtatcct ccatgtattt gcctgccaga tattcctgtg acatggtaag
    18421 catttccaga gaataatccg gtgcatattt atgaaggatc tctgcgcttt ccttcggatg
    18481 ttcgattgca taccgatatc cctttgcagt agcctccagg aatttcttcg tcatttccgg
    18541 cttatttttc agcgtatcct cactggcgat aataacaggg gtatagtaat ccagacgctt
    18601 gtccagctga cgcagctcca tatagttgat aggaaaatca ttcagccggc attttatatt
    18661 gtcccatccc tcaaaaaacc acataacgtc aaccttatcc ttcagcgcct caaagccgga
    18721 gccgtctgaa atcaccatat ccagctttga aaaatcccct ccgtcctttg ccatcactgc
    18781 ctttaacacg gcctcctctc ccggaccgcc ccagcctgca taggtttttc cctcaaaatc
    18841 cttcggagaa tggatatcct tatccccata ggtcacaaag cctgaggtat tgtgctggat
    18901 caaagctgca accgctttaa ccggaagagg atcggccgag gttcgggcta tggtcacatc
    18961 ctcctgatag ctgatgccga aatctccctt tcccacagca atcagcgtag ctgtggctcc
    19021 ctctgtaggc tccacgatac tcacatccat tccgtcctct ttgtaatacc cctgatccag
    19081 agccacatac attcctgtgt ggttggtatt ggcaacataa tccagaatta ccgtaacatt
    19141 ctcggggctc tctccttctg ccgccttctc agaagcagtg tctgcacagc cggacaatcc
    19201 tgctgcaact gctgcagcca aaatccctac cgctatcttt ctcacaaata ttccatagtt
    19261 tcccatctct tatttcctcc tctttctttc atagggaagg cagatccgcc caagcaggcg
    19321 cacagcccca ttcacgcaca ggctgagacc aatgatcaca gccacacagg caaaaacctt
    19381 gtccagcata tatccgtttt ttacccgcag caggtaatat ccaagtccct gctgggaacc
    19441 gatccactct cctaccacag ccccgctgat actgtaagtt gcagccacct taagaccgga
    19501 cagcagcgca ggaagggcag caggaatttt taccagacgg tacgctcccc attttcccgc
    19561 accataggaa cgtaccaggt gaacgtattc ctcatccaca cgggacaggc cgtctgaaaa
    19621 gctcaccgcc acaggaaaaa agcacatcaa aaccacggta agaatcttcg ggccgggacc
    19681 aaagcccatg tagatgatca aaatcggggc aagtacgatc atgggcacgg tctgagtaac
    19741 gaccaaaaga gggtatatgc ctctttgcac tgccggaaac cggtccataa ggattcccag
    19801 ggccagtgcc aaaatcagag agatcagaat accggccagt gcctccaata ccgtcacggc
    19861 tccatgggaa accagaaccg gccattcttc tcccaaagca gcagctatgg ctgtaggtgc
    19921 gggaagaata tagagaggga tatgaaacag ccttaccgcc agctcccaca ggagtaaaat
    19981 caccagaata taacctgccg gagccatccg gctgcttacc tgcttcatgt tcaatcttat
    20041 cctccttata tgttaaaagg cctccatccc gcaggacaga ggccaaaagc attcatatac
    20101 tctgtatttc cctacgttgg cattatccaa atcaggttat gggtcgaaat tacaatttcc
    20161 tctcagcctg tatacaggct cccctgttgt cttattcctc tgccgcggca tttacctccg
    20221 gcagcagcat gtaattgttg tcgcggtccg agtcgtcgat catggcgtcc agctccttta
    20281 acagataact gtccggcata tttctctccc accagcggta gcccttggaa cttcttatac
    20341 cctttgcatc gatgcggcgt ccggcataat aatcaatata aacggaagca tacacctctg
    20401 ccatggagtg ctccacgtca tttcccagat tctttacaac accccggatc tggtcaatga
    20461 tcagcttctg gatatagcgc tccgaccaac cctcaaaatc cagaagatcc ggattctggc
    20521 ttccagtacg cgccgcccag gccgatacat ccactgcctt tgtgctgtat tccatactgc
    20581 cgtcccgctt ccattccaga agcgcatact gacagggcgg tatgctgagt gcgtccgtga
    20641 caatctccat gattccgtat gaatcatctg ccgctcccgg ttctgcctta tgcttgcgga
    20701 tccgctgaac atgaaggtgg ccgcttaagt acagcggcag ttcatacttc tggaacagct
    20761 cgatcacatc gccattgttg ttcatggcac actgggtcgt gtacatccgg ctctgagcca
    20821 gaagattgtg gtggccgatg ggaagaacgt tcatcccatc ttcctttgcc tttttaagct
    20881 gttcatctgc ccaccggagc gtttccacct taagctgccc ttctaccata ttcttaggct
    20941 catactggca ggtatccagc atcaggagac gatttctctc atccagctga tagacatagc
    21001 tcaaagaagc atcatcccgg ctgatagcct ggtcgtagcc gtacatatgg taaatattgt
    21061 aaaattcttc cggcgtcacc ggatctgtct ctgccttttc ctcaccgaaa tacacggctg
    21121 catggggatt gttgatatca tggtttccgg gaacaataag taccggaatc ccggcgtcct
    21181 gtacccgcat aagccctctg gccagctcct catggtttat cttttcccca ttcatggtga
    21241 tatcgccgct taaaaccaga gccgagggat gttcctctat gacctgatcc ataaatgctt
    21301 ctaaaagctc cggcagatat tccaccacct tgccgtc
//
LOCUS       DBMDUH010000029        21091 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_1570_length_21091_cov_5.387137, whole genome shotgun sequence.
ACCESSION   DBMDUH010000029 DBMDUH010000000
VERSION     DBMDUH010000029.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 21091)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 21091)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21091
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1570_length_21091_cov_5.387137"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="ACZPCG_04155"
     CDS             complement(<1..369)
                     /locus_tag="ACZPCG_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009262427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HIR1284302.1"
                     /translation="MIIPDWNGKPYHSLDYELKHTFGRKLYKLSLDGGMTCPNRDGTC
                     GSRGCIFCSEGGSGDFAAPLGGDVWRQIEAAKERIRRKMPPADGSADYIAYFQSFTNT
                     YASTDYLSQLFSRAISHPEIA"
     gene            563..1858
                     /locus_tag="ACZPCG_04160"
     CDS             563..1858
                     /locus_tag="ACZPCG_04160"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006779158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005534 - galactose binding [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HIR1284303.1"
                     /translation="MNVKDTIRLLKSEKASALMAELYGKEGAAENETRYEKVLTGFEQ
                     TFGDRENILLFSSPGRTEISGNHTDHNHGKVLAGSINLDCVGAAAVNYSNLVHIISET
                     YDQDITIDLNQLEPSAKKAGTNDLIKGLLKGFKEAGYSIGGFDAYVTSNVISAAGVSS
                     SASFEMLLCSMLNTFFNEGRMDSVAYAHIGKYSENIYWDKASGLLDQMACAVGGLITI
                     DFTEPAVPEVEKINFDFESQDHSLIIVQTGKGHADLSADYSSVPNEMKKVAQFFGKEV
                     LSQVTEEQVIEKLSEVRSFAGDRSVLRALHFFEENKRVEEEVKALKEGNFEKFLKNIT
                     ASGNSSWKWLQNCFTNSAVQEQGITIALALTELFIAEKQRGACRVHGGGFAGVIMVML
                     PNDLTDEYIRYIEGCLGEGCAYRMSIRPYGAVCINELLK"
     gene            complement(1941..3398)
                     /locus_tag="ACZPCG_04165"
     CDS             complement(1941..3398)
                     /locus_tag="ACZPCG_04165"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002575051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HIR1284304.1"
                     /translation="MDHRNLTLLTDLYELTMMQGYFKEKDANETVIFDMFYRNNPHGN
                     GFAVCAGLDQVIEYIRDLHFDKSDIAYLRSVGIFEEDFLEYLRDFRFTGDIYAIPEGT
                     VVFPREPLVKVTAPIMQAQLVETALLNIINHQSLIATKTERIVYAAKGDGVMEFGLRR
                     AQGPDAGTYGARAAMIAGCIGTSNVLCGKMFDVPVKGTHAHSWIMSFPDELTAFRTYA
                     KLYPSACILLVDTYDTLNSGIPNAIRVFKEMREEGIPLTFYGIRLDSGDLAYLSKKAK
                     KMLDEAGFPDAIISASNDLDEYLIDSLKMQGAAINSWGVGTNLITAKDCPSFGGVYKL
                     AAVKDKTTGEFIPKIKLSENAEKITNPGNKTIQRIYEKETGKIIADLICLVDEKFDTN
                     NSLLLFDPIETWKKTLLAPRTYTMRELLVPVFLNGTCVYETPKVMDIQAYCKKELDTL
                     WDESKRLVNPHKVHVDLSNELWHMKNQLLDTYHRK"
     gene            3601..4944
                     /locus_tag="ACZPCG_04170"
     CDS             3601..4944
                     /locus_tag="ACZPCG_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006780476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HIR1284305.1"
                     /translation="MRYSGYELLWMFAVYSVAGWCLEVCAAAAKQKKFVNRGYLNGPF
                     CPVYGLTSAAFGIVLPELREHLIFLFLGGMIIAAFAEYICGFLLERISGQKWWDYSGH
                     RFSARGYICLPASLFWGICAVLCIRLFDPLLVKLIYMLPQRAGRLILTAVLIAAFLDG
                     LGSSAAVLQMNYFLGRAAFEVSGRLYGLSSFLENALTERIRRRMVKAYPSLKERKRAE
                     NGGIDEKTAVFARGCCFYKLAMLFFIGAFLGDITETIFCYTRTGIIMSRSSVVYGPFS
                     IVWGLGCVLLTAFLYPYREKNDRYIFLAGTILGGAYEYICSVFSELVFGTVFWDYSAI
                     PFNLGGRINLLYCFFWGIVAVVWLKMLYPILSGWIEKLPVKCGKLLSNSLILFMVFDM
                     ALSGLALGRYSARQDEKTAVSQTVSGEEGGLNGWLDRHFPDERMKRIYPNAKFVD"
     gene            5342..8476
                     /gene="ileS"
                     /locus_tag="ACZPCG_04175"
     CDS             5342..8476
                     /gene="ileS"
                     /locus_tag="ACZPCG_04175"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HIR1284306.1"
                     /translation="MYNKVPTDLKFVEREKEVEKFWADEHIFEKSIKMREGCKPYVFY
                     DGPPTANGKPHIGHVETRVFKDMIPRYRTMKGYMVPRKAGWDTHGLPVELEVEKKLGL
                     DGKDQIEKYGLEPFIKECKESVWKYKGMWEDFSSTVGFWADMDHPYVTYDNNFIESEW
                     WALKQIWDKGLLYKGFKVVPYCPRCGTPLSSHEVAQGYKDVKERSAIARFKVKDEDAY
                     ILAWTTTPWTLPSNVALCVNPNEEYVKVKTADGYTYYMAHALVEKVLGEDVEVLETYQ
                     GKELEFKEYEPLYDCSKPYCEKQHKKGHYVVCDTYVTLTDGTGIVHIAPAFGEDDANV
                     GRKYDLPFVQLVDAKGDMTEDTPFGGRFVKDADPLVLKDLEERGLLFSAPSFEHSYPH
                     CWRCGTPLIYYARESWFIKMTAVKDDLIRNNNTVNWVPESIGKGRFGDWLENIQDWGI
                     SRNRYWGTPLNIWECEGCGHQHSVGSIEELKSLSDNCPDEIELHRPYIDAVTIKCPHC
                     GGVMKRVPEVIDCWFDSGAMPFAQHHYPFENKELFESQFPAQFISEAVDQTRGWFYSL
                     LAESTLLFNKAPYENVVVMGLVLDENGQKMSKSKGNAVDPFEALATHGADAIHWFFYT
                     CSAPWLPKRYQDKAVTEGQRKFMGTLWNTYAFWVLYANIDQFDATKYSLEYDKLPVMD
                     RWLLSKMNSMVKTVDNSLMNYQIPEAARALQEFVDDMSNWYVRRSRERFWAKGMEQDK
                     INAYMTLYTALVTVAKAAAPMIPFMTEQIYQNIVRNIDKNAPESIHLCDYPEVKEEWI
                     DLALESDMDEVLDTVVMGRAARNTANIKNRQPIAQMYVKADHELSEFYVKIIEEELNV
                     KKVTFTEDVRAFTSYTFKPQLKTVGPKYGKLLGRIRQALTELDGSAAMDTLNESGALT
                     FDFDGQEVVLTKDDLLIDVAKKDGYVTEEDNYVTVVLDTNLTPELIEEGFVREVISKI
                     QTMRKEAGFEVMDHIAVYQDGNERIAELVKANADEIKTEVMADHIHLGQMKGFVKEWN
                     INGESVMLGVEKLM"
     gene            8567..11014
                     /locus_tag="ACZPCG_04180"
     CDS             8567..11014
                     /locus_tag="ACZPCG_04180"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HIR1284307.1"
                     /translation="MEFDKATFKKSVMYNVKNMFRRTIDEATPQQVFQAVAFAVKDVI
                     IDEWIATHKEYEKKDAKTVYYLSMEFLMGRALGNNIINICARKEIAEVLEELGFDLNV
                     IEDQEPDAALGNGGLGRLAACFLDSLATLGYPAYGCGIRYKYGMFKQKIENGYQVEVP
                     DNWLKNGNPFEIRRPEYAVEVKFGGYVRIENRDGMNHFVQDGYQSVRAVPYDLPVIGY
                     GNNVVNTLRIWDAEPINTFNLDSFDRGDYQKAVEQENLAKTIVEVLYPNDNHYAGKEL
                     RLKQQYFFISASVQRAVLKYKELHGTIHGFQDKVVFQLNDTHPTVAIPELMRILLDEE
                     GLTWEEAWDITTKTCAYTNHTIMSEALEKWPIDLFSRLLPRIYQIVEEINRRFVNEIQ
                     ARYPGDQEKIRQMAVVYDGQVRMAYMAIVASFSVNGVARLHTEILKHQELKDFYEMMP
                     EKFNNKTNGITQRRFLLHGNPLLADWVTSKIGDQWITDLPHIAELLPCAEDKECQKEF
                     MQIKYENKVRLAKYIKEHNGIDVDPRSIFDVQVKRLHEYKRQLMNILHVMYLYNQLKD
                     HPDMDMVPRTFIFGAKAAAGYKRAKLTIKLINAVADVVNNDKSIGGRIKVVFIEDYRV
                     SNAEIIFAAADVSEQISTASKEASGTGNMKFMLNGALTLGTMDGANVEIVEEVGNENA
                     FIFGMSSDEVIAHEKNRDYQPMDIFNNDPEIRRVLMQLVNGFYSPENPELFRDIYDSL
                     LNTKSSDRADTYFILKDFRSYAEAHKKIDQAYRNSSWWAKAAILNVGHSGKFTSDRTI
                     EEYVRDIWHLKKITVEV"
     gene            11141..12001
                     /gene="metF"
                     /locus_tag="ACZPCG_04185"
     CDS             11141..12001
                     /gene="metF"
                     /locus_tag="ACZPCG_04185"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase [NAD(P)H] activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HIR1284308.1"
                     /translation="MKIRDILAEGKPTLSFEVFPPKTQDAFASVEKAAEEIARLHPAF
                     MSVTYGAGGGTSDYTVKIASDLQENCGVTPLAHLTCVSSTREKVQQVLNELKEHKIEN
                     ILALRGDIPQDGQTPQVYHYASELIREIREAGDFCIGAACYPEGHVESANKSVDMDYL
                     KQKVEAGCDFVTTQMFFDNSILYSYLYRIREKGITVPVIAGIMPITNVKQVKRSIQMS
                     GCHMPARFMSLVDKFGDDPAAMKQAGIAYATDQIIDLIANGIRGIHVYTMNKPDVAAK
                     IRENLSDIVK"
     gene            12030..12701
                     /locus_tag="ACZPCG_04190"
     CDS             12030..12701
                     /locus_tag="ACZPCG_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vitamin B12 dependent methionine synthase
                     activation subunit"
                     /protein_id="HIR1284309.1"
                     /translation="MDISSVSRREVLRYLGYRGQAEPDRAVADEIEMCLGLLAEKAEP
                     RHRVRSFLLELGPEHEIRFDRVHTSSRQLSKNLAGCGEILVFAATLGLGTDYLIQRYN
                     RLQMSRAVILQAAAAAMIEEYCDRVCRELKAAYEAKGLYLRPRFSPGYGDFPLECQPY
                     LLECVDGGKQLGIRLTGGYLMMPSKSVTAVMGLGRTPCGCEVKGCEACGKKDCAYRRQ
                     TGSED"
     gene            12705..15116
                     /locus_tag="ACZPCG_04195"
     CDS             12705..15116
                     /locus_tag="ACZPCG_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HIR1284310.1"
                     /translation="MNILQEMRQRRLFFDGGMGSLLQAKGLKAGELPERWNLTHPEEI
                     CGIHRMYLQAGSDLITTNTFGANRLKYGEELEKIVSEAVSHAKEAVRAEGHGYVALDL
                     GPTGKLLKPLGDLEFEDAVEIYKETVSYGARAGADLVLIETMSDCYELKAAVLAAKEA
                     GFSPETGERLPIFATVIFDEKGKLLTGGDVSSTVALLEGLGVDVLGINCGLGPVQMKG
                     IVEELLSVSSTPVLVNPNAGLPRSENGKTVYDIDAVRFGEVMEEIVKMGAVIVGGCCG
                     TGSEHIRETVKRCKDLPVMWPEKKNRTVVSSYSQAVVFDKKTVIIGERINPTGKSKFK
                     QALRDHNLEYILREGVTQQDNGADILDVNVGLPEIDEPSMMEAVVKELQAVIDLPLQI
                     DTSNPQAMERALRIYNGKALINSVNGKKEVMDQIFPLVAKYGGVVVGLCLDEDGIPET
                     AEGRIAVGRKIIKTAASYGIGPEDIILDGLCMTVSSDNKGALTTLETLRRIRDELGGK
                     SVLGVSNISFGLPQREIINSSFFTMAMECGLNGAIINPNSEAMMRAYYSFNALMDRDP
                     QCSRYIDIYGSETGGLGQTLSRTKDTGKGKEGDSLGILIEKGLKEAAAETVAALLEEK
                     DALDIINGEMIPALDRVGKGFEKGSVFLPQLLMSAEAAKAAFEVIKAKMEGSGQGQEK
                     KGTIILATVKGDIHDIGKNIVKVLLENYSYEVIDLGKDVPPERIVEEAVKRHVPLVGL
                     SALMTTTVPSMEETIRRLRKEASWVKVMVGGAVLTKEYADTIGADAYCRDAMASVHYA
                     ETVIG"
     gene            15138..15953
                     /locus_tag="ACZPCG_04200"
     CDS             15138..15953
                     /locus_tag="ACZPCG_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007714664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HIR1284311.1"
                     /translation="MGHNTRENTKILLKGAWVGGTMTVPGVSGGTMAMVLGIYDRLIS
                     SVSRVFKEPKQSIPFLLWFALGGAGGILLFSRGISLLLSSRWEVPARFFFLGAVGGGI
                     PLIFKEAGIKRFGSGDAVFVGIGILSALLIGLIPEGLFTPGAGGVSDILIQALGGVII
                     AVALVLPGISASQMLYMLGIYESTLKAVGNMDIAALVPLAAGGLLGTFFSAKVLEGLL
                     EKHTRPTFLLILGFMFASLPELFPGIPAGAEAVYSLVAVGAGFMLLYGIGRMN"
     gene            16040..16717
                     /locus_tag="ACZPCG_04205"
     CDS             16040..16717
                     /locus_tag="ACZPCG_04205"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002575041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1284312.1"
                     /translation="MYKCCMFDLDGTLVNSIYALKRSVDGTLAHFGLGPITLEDTKTF
                     VGDGYKKLVERALKAYGDPELVHFEEALNVYNRVFKECCLYRVEAYEGIPELLAFLKE
                     NHILCTVLSNKPHVRTIENINYVFGPDVFDRVYGERESAGIKKKPSPEGVEALLEELG
                     ISKEECLYLGDTNTDMETGKNSQVDTVGVLWGFRSRDELKVYEPALLAEHPMDVAEFI
                     KGVNHIE"
     gene            16710..17771
                     /locus_tag="ACZPCG_04210"
     CDS             16710..17771
                     /locus_tag="ACZPCG_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003509268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HIR1284313.1"
                     /translation="MNRQKKRSGAWTKTGLLKTGILFFALLLGSLAFTGCAKRESPGI
                     RGEQGGDDRLFVVTTIFPYYDFVRQIGGDRVRLNLVVPAGMDSHSFEPTPADMITIQK
                     ADLMICNGGAMEQWVDQVAASLDTSRLRRLTMMDYVDVVEEEVVEGMEKEEHGHEHSC
                     EEEEAGEFDAHDGQELDIEYDEHIWTSPVNSMKIVEIISDTLAEMDPANEAYYRENAA
                     SYLEELSALDEQFREIVSNGRHRMMVLADKFPLRYFADTYGLSYRAAFSGCSTDTEPS
                     ARTIAYLIDRVRENDLKAVYYLELSSHRTADIISEETGTEPLLFHSCHNVSRREFDEG
                     VTYLQLMRQNAEHLKKGFE"
     gene            17778..18548
                     /locus_tag="ACZPCG_04215"
     CDS             17778..18548
                     /locus_tag="ACZPCG_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007714657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HIR1284314.1"
                     /translation="MEPLIKCEHVDFGYENQDAVVDVNMEVCPGDYLCIVGENGSGKS
                     TLMKGLLGLLKPSAGVLTVSEELKHTGIGYLPQQTAAQKDFPASVQEVVLSGCLNRRG
                     RRPFYSRTEKAVAAENMERLGITPVAKQCYRELSGGQQQRVLIARALCATTRLLILDE
                     PITGLDPMAIQDFYAMIRRLNREDGVAILMVSHDLRNAVEEANKILHLQKQVLFYGPA
                     HDYMNSQAAGHFFHEKEQGQCIPTAVCRKGGCCHEHTK"
     gene            18532..19350
                     /locus_tag="ACZPCG_04220"
     CDS             18532..19350
                     /locus_tag="ACZPCG_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006566958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HIR1284315.1"
                     /translation="MNILSEMLSYPFLVRALVGGMLVSLCASLLGVSLVLKRYSMIGD
                     GLSHVSFGALSIALAMGWSPLKVSIPVVVLAAFFLLRITENSRIKSDAAIAMISASAL
                     AIGIIVTSLTTGMTTDVSSYMFGSILAMTKEDMALAVVLSLVVLGLFVICYNQMFAVT
                     FDENFARATGVNVGFYNMVISVLTAVTIVLGMRMMGAMLISSLVIFPCLTSMRVFKSF
                     GGVMISSGLLSLVCFLLGMMASYQFSTPAGASVVVVNLAAFVIFGAWQMLGRRK"
     gene            19607..20728
                     /locus_tag="ACZPCG_04225"
     CDS             19607..20728
                     /locus_tag="ACZPCG_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CobW family GTP-binding protein"
                     /protein_id="HIR1284316.1"
                     /translation="MTKIDIISGFLGAGKTTFIKKLLQEAISGEQVVLIENEFGEIGI
                     DGGFLKDSGIEIREMNSGCICCSLVGDFGKSLAEVLTTYKPDRIIIEPSGVGKLSDVM
                     KAVIDVSADMDVALNSAVTIVDAAKCKMYMKNFGEFFNNQIENAGTIVLSRTDITDPA
                     KIQKDVEQIREKNPKAAVVTTPLGELGGDQLLEIIEKRDTMLDDLMAEVREHHHHDEE
                     CGCGEHHHDHEGHDHHHGHEHGESCTCGCHEHEEHEHHHHHDHGEDCTCGCHDHEHHH
                     HHHADEVFTSWGMETIVPVTRDQLEDILKRLADTKEFGDVLRAKGMLPTENPGEWLYF
                     DLVPQQYEIRQGRPDYTGKVCVIGASLKEEELNSLFGRG"
     gene            20742..>21091
                     /locus_tag="ACZPCG_04230"
     CDS             20742..>21091
                     /locus_tag="ACZPCG_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008716801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR1284317.1"
                     /translation="MGNEIEDNVMPLFLINGFLEAGKTQFLEFTMEQDYFQTEGKTLL
                     IVCEEGDTEYDEKKLKKNRTAVVYVDDFSKLTPEYLNELKVIYRPERVIMEWNGMWNQ
                     DELKLPEDWFIYQQ"
BASE COUNT         5559 a         4417 c         5876 g         5239 t
ORIGIN      
        1 cgcaatttcc ggatgggaaa tggctctgga aaacagctgg gacagataat cagtggaggc
       61 ataggtgttg gtaaaggact gaaaataggc aatgtaatcg gcgctgccgt ctgctggagg
      121 cattttcctg cggatccgtt cctttgcagc ttcaatctgt ctccatacat cgcctcctaa
      181 aggcgcggca aagtctccgg atccaccctc gctgcagaaa atgcagcctc ggctgccgca
      241 ggtgccgtcc cggttggggc aggtcatgcc gccgtctaag gagagcttgt aaagtttgcg
      301 cccaaaggta tgttttaatt cataatccag agagtggtat ggctttccat tccagtcggg
      361 tattatcatg ggagtacatc ctttctgtaa aagtttgtat catggtatta tatcagaaat
      421 acggagtgtg tccagaaaat tgtataaata caggaaaata ttgtattgaa ggaggatgct
      481 tttttgtgat acactgtttt catagggttc ggagccctca ggagatatgt atttgaatga
      541 aagaaggaga ggtaaagcag ggatgaatgt aaaagatacc atcaggcttt taaagagtga
      601 aaaagcctca gcactcatgg cagagcttta tggaaaagaa ggagcggctg agaatgagac
      661 ccgctatgaa aaggttctta caggctttga acagacattt ggcgataggg aaaatattct
      721 tctgttctca tcaccgggcc gtacggaaat cagcggaaac catacagacc ataaccatgg
      781 aaaggtactt gcaggcagca ttaacctgga ctgcgtaggt gcggctgctg ttaattattc
      841 taatctggtc catatcatca gcgaaaccta tgatcaggat atcaccattg atttaaacca
      901 gttggagcca agcgcaaaaa aagcaggaac taatgatctg atcaagggtc tgttaaaagg
      961 gtttaaggaa gcaggctaca gtatcggcgg ttttgacgcc tatgtgacca gcaatgtgat
     1021 cagcgctgca ggcgtgagtt catccgcttc ctttgagatg cttttatgtt ccatgctgaa
     1081 tacatttttc aacgagggcc gcatggacag cgtagcttat gcccatatcg gaaaatattc
     1141 tgagaatatt tactgggata aggcatccgg acttttagat cagatggcct gcgccgtggg
     1201 cggtctgatc accattgact ttacagaacc tgcggttcct gaggtagaaa agatcaactt
     1261 tgactttgaa tctcaggatc acagcctgat cattgtgcag actggaaagg gccatgcaga
     1321 tttaagcgca gattattctt cggttcccaa cgagatgaaa aaggtggctc agttcttcgg
     1381 gaaagaggta ctttcccagg tgacggagga acaggtgatt gaaaagctga gcgaggtaag
     1441 aagctttgca ggtgaccgtt ctgtgctccg cgctctccat ttctttgaag aaaacaagag
     1501 agtggaagag gaagtaaagg ccttaaagga aggaaacttt gagaagttcc ttaaaaatat
     1561 caccgcttcc ggcaattctt cctggaaatg gcttcagaac tgctttacaa acagtgctgt
     1621 tcaggagcag ggaattacca ttgcgctggc tcttacagag ctttttatcg cagaaaagca
     1681 gaggggcgcc tgcagagttc atggaggcgg ttttgccggc gtcattatgg ttatgctgcc
     1741 aaatgacctg acagatgagt atatccgcta tatcgagggc tgtctggggg agggctgcgc
     1801 ttacagaatg agtatccgtc catacggagc agtctgcatt aatgaactgc tgaaataaga
     1861 taatataaaa accggctttc tgccccatat caggcggtac agactgatat gggggaaagc
     1921 cggtttttat tcgtatttca tcattttctg tgataagtat caagaagctg atttttcata
     1981 tgccacagtt cattggacag atccacatga accttgtggg gatttacaag acgcttggac
     2041 tcgtcccaca gggtatccag ttcctttttg cagtaagcct ggatgtccat tacctttgga
     2101 gtttcgtata cgcaggttcc gtttaggaaa acagggacaa ggagctcacg catggtatag
     2161 gttctgggag cgaggagtgt ttttttccat gtctcaatgg gatcaaagag aaggagcgaa
     2221 ttgttagtgt cgaatttctc atccaccagg cagatgagat cagcgataat ctttccggtt
     2281 tctttttcgt aaatccgctg gatcgtcttg ttgccggggt tggtaatttt ttccgcattc
     2341 tcagagagct tgatcttggg aataaattcg ccggtggttt tgtcctttac cgcagccagc
     2401 ttataaacgc ctccgaagga agggcagtcc tttgcggtga tcaggttggt tcccactccc
     2461 caggaattga tggcagctcc ctgcatcttt aggctgtcaa tgagatattc gtcaaggtca
     2521 ttggaagcag agataatcgc atccggaaag ccggcttcat ccagcatttt tttcgctttt
     2581 ttggaaagat aggccagatc gccgctgtct aagcggatgc cgtaaaaggt aagggggatt
     2641 ccttcttcac gcatttcttt aaatacccgg atggcgtttg gaataccgga atttaaggta
     2701 tcgtaggtgt ctaccagaag gatgcaggca gacggataga gctttgcata ggtacggaag
     2761 gcggtaagtt catccgggaa gctcatgatc cagctgtgag catgggtgcc ctttacaggt
     2821 acgtcaaaca tttttccgca gagcacattg gaggtgccga tgcagcctgc gatcatggcg
     2881 gctcttgccc cataggtgcc ggcgtccggc ccctgggcac ggcgcagtcc aaattccatc
     2941 acgccgtcgc ccttggcggc atagacgatg cgctctgttt tagtggcgat caggctctgg
     3001 tgattgatga tattaagaag ggctgtttct accagctgtg cctgcatgat cggggcggtc
     3061 acctttacaa gaggttctct ggggaaaaca accgtacctt caggaatggc atagatatca
     3121 cccgtaaaac ggaagtcccg gagatattcc agaaagtctt cttcaaagat tcctacgctt
     3181 ctgagatatg cgatatcgct tttatcgaaa tgaagatccc tgatatattc aatcacctga
     3241 tccagtccgg cgcagacggc aaacccgttt ccgtgcggat tgttccggta aaacatatcg
     3301 aaaatgacgg tttcattggc gtctttttct ttaaaatatc cctgcatcat ggtaagttca
     3361 tacaggtctg tcagcagtgt aagatttctg tgatccataa ggttttcctc cctggtatgt
     3421 ccccgtcttc ccttaagggc cggcggaagg ctgtaagtta attgacagta gtatagcata
     3481 aattttacaa aaggaaaacg aaatgttaaa aatataacga ttctgtccgt atggtgaaat
     3541 ggcatattct gtggtacaat tcataagaga atgaaaagaa ctgtaaaaag gaggcggcct
     3601 atgaggtatt ccggatatga gctgttatgg atgtttgcgg tctactcggt cgcgggctgg
     3661 tgcctggagg tgtgtgcggc tgcggccaag cagaaaaaat ttgtcaacag aggctattta
     3721 aacggaccct tctgtccagt ctacggattg acgtctgcgg cctttgggat tgttctgccg
     3781 gaacttaggg agcatttgat tttccttttt ctgggtggca tgatcatagc agcctttgca
     3841 gaatacatct gcggttttct gctggagcgg atttccggcc agaaatggtg ggattattcc
     3901 ggacacaggt tttctgcaag agggtatatc tgtctgccgg cctctctttt ctggggaatc
     3961 tgtgccgtgc tttgtatccg gctgtttgac ccgcttctgg taaagcttat ttatatgctt
     4021 ccccaaaggg ccggaaggct tattctgaca gctgtattga ttgcagcatt tttagatggc
     4081 ctgggttctt cggcagctgt cctgcagatg aactattttc tgggacgggc agcctttgag
     4141 gtcagcggac ggctttatgg gctgtcgtcc tttcttgaaa atgccctcac agagcggatc
     4201 agacgcagga tggtaaaagc gtatcccagc ctgaaagaaa ggaagagggc ggagaatggg
     4261 ggcatagatg aaaaaacagc cgtttttgcc agaggctgct gtttttacaa gctggcaatg
     4321 ctgtttttta tcggtgcttt tctgggggat attacagaaa cgattttctg ctataccaga
     4381 accgggatca tcatgagccg cagcagcgtg gtatacggcc ctttcagtat tgtatggggt
     4441 ctgggctgtg tgctcctcac tgcgtttctt tatccctaca gagagaaaaa tgaccgttat
     4501 atctttcttg cgggaacgat ccttggagga gcttacgaat acatatgcag tgtattttca
     4561 gagcttgtgt tcggtacggt attctgggat tacagcgcca ttccctttaa tctgggaggc
     4621 cgtatcaatc tgctgtattg ttttttctgg gggatcgtgg cggtggtctg gctaaagatg
     4681 ctctatccga tactgtcggg atggattgag aagcttccgg tcaaatgtgg aaagcttctc
     4741 agcaatagtc tgatcctttt tatggtcttt gatatggctc tctccggcct tgctctgggg
     4801 cggtattctg caagacagga tgaaaagaca gccgtaagcc agacggtttc cggggaagaa
     4861 ggcgggctga atggctggct ggaccgccat tttccggatg aacgtatgaa acgtatctat
     4921 ccgaatgcca aatttgtaga ttagtattct gtattcgctt ttttgaggac ttttttaatc
     4981 tgccgttgac attttaaagc ccagtcatta taataaagac gttataaggt aaaggctgtg
     5041 aaggagacag tagctttccc cagagaccaa agagagccgg tggcggtgga aatccggtgt
     5101 caaaagggat gtttgtgaag atcactccgg agccgccggc tgaacgtttt tagtaggcac
     5161 ggccgttttt cctgcgttag aggaactcat atgacagtat gcagtaacag gtggttaaac
     5221 gaatccaaga ctttcgtcct tttacagggc gggagtcttt ttttatttca taggaaaggg
     5281 atccctatga aatattcttt cctgtgaggc atcgaacaaa ttcaaatgga ggtagaaaaa
     5341 gatgtacaac aaagtcccta ctgacttaaa gtttgtagaa cgggaaaaag aagtagaaaa
     5401 gttctgggca gatgaacaca tttttgaaaa gagcatcaag atgcgtgaag gatgcaagcc
     5461 ttatgtgttt tatgacggac cgccaactgc caacggaaaa cctcatatcg gccatgttga
     5521 gacccgtgta tttaaggata tgatccccag atacagaacc atgaagggat atatggttcc
     5581 aagaaaggcc ggctgggata cccacggact tccggtcgag ctggaagtgg aaaagaagct
     5641 gggcctggac ggaaaggatc agatcgagaa gtacggtctg gagcctttta tcaaggagtg
     5701 taaggaaagt gtctggaagt acaagggaat gtgggaggat ttctccagca ccgtaggctt
     5761 ctgggccgac atggatcatc catacgttac ttatgacaat aactttatcg agtctgagtg
     5821 gtgggcctta aagcagattt gggacaaggg cctgttatat aagggcttta aggtagtacc
     5881 ctactgtcca cgctgcggaa ctcctctgtc ctcccatgag gtagctcagg gctataagga
     5941 tgtaaaggaa cgctccgcca ttgcccggtt taaggtaaag gatgaggatg cttacattct
     6001 ggcatggacc accactccct ggactcttcc aagcaacgtg gctctctgcg tcaacccaaa
     6061 tgaggaatat gtaaaggtaa agaccgcaga cggttatacc tactatatgg ctcacgcact
     6121 ggtagagaag gtgctgggcg aggatgtgga agtccttgaa acctatcagg gcaaggagct
     6181 ggaatttaag gagtacgagc ctctttacga ctgctctaag ccgtactgcg aaaagcagca
     6241 caaaaagggc cattacgttg tctgcgatac ctatgtaacg ctgacagacg gtactggaat
     6301 cgtacacatc gctcctgctt tcggtgagga cgacgctaat gtgggccgta aatatgacct
     6361 gcccttcgta cagctggtgg acgcaaaggg cgacatgaca gaggacactc cgttcggcgg
     6421 cagatttgta aaggatgcgg atcctctggt attaaaggat ctggaggaga gagggctgtt
     6481 attctccgct cccagctttg agcacagcta tcctcactgc tggcgctgcg gaacacctct
     6541 gatctactat gcaagagagt catggttcat caagatgacc gctgtaaagg atgatctgat
     6601 ccgcaataac aatactgtaa actgggtgcc tgagtccatc ggaaagggcc gcttcggcga
     6661 ctggctggag aatatccagg actggggcat ttccagaaac agatactggg gtacgccgct
     6721 taatatctgg gagtgcgagg gctgcggcca tcagcattcc gtaggaagca tcgaggagtt
     6781 aaagagcctt tctgacaact gccccgatga gatcgagctt caccgtcctt atattgacgc
     6841 agtgaccatc aagtgccctc actgcggcgg cgtgatgaag agagtgcctg aggtaattga
     6901 ctgctggttc gattcaggag ccatgccctt tgcacagcat cattatccgt ttgagaataa
     6961 agagcttttt gagtctcagt tcccggctca gttcatttcc gaggctgtgg atcagactag
     7021 aggctggttc tactcccttc ttgcagagtc caccctgctc tttaacaagg cgccttatga
     7081 aaatgtagtt gttatgggtc ttgtattgga cgagaacgga cagaagatga gcaagtccaa
     7141 gggaaatgcc gttgatccct ttgaggctct ggcaacacat ggtgcagacg caatccactg
     7201 gttcttctat acctgcagcg cgccctggct tccaaagaga taccaggata aggctgtgac
     7261 cgagggccag agaaagttca tgggaacgct gtggaacacc tacgcattct gggtactgta
     7321 tgccaatatt gaccagtttg atgcaaccaa atacagcctg gagtatgaca agctgcccgt
     7381 tatggaccgc tggctcttat ccaagatgaa ctctatggta aagaccgttg acaacagcct
     7441 gatgaattac cagatccctg aggcagcaag agcccttcag gagtttgtag acgatatgag
     7501 caactggtat gtgcgcagaa gccgtgagcg tttctgggca aagggcatgg agcaggataa
     7561 gatcaatgct tatatgacac tttataccgc tcttgtaacc gttgcaaagg cggctgcccc
     7621 aatgatcccc ttcatgacag agcagatcta ccagaatatt gtaagaaata ttgataagaa
     7681 tgcgcctgag agcatccatc tgtgcgatta tcctgaggta aaggaagagt ggatcgacct
     7741 ggccttagag tctgatatgg atgaggtgct ggatacggtt gttatgggcc gtgccgccag
     7801 aaatacagcc aatatcaaga accgtcagcc tatcgcccag atgtacgtaa aggcagacca
     7861 tgagctgtcc gagttctatg taaagatcat tgaggaagag ttaaatgtta aaaaggtaac
     7921 ctttacagag gatgtacggg catttacctc ctataccttt aagcctcagt taaagaccgt
     7981 agggccaaag tatggaaagc ttctcggaag gatccgacag gctctgacag agctggacgg
     8041 cagcgctgcc atggatacct taaatgaatc cggcgcactg acctttgatt ttgacggcca
     8101 ggaggttgtt cttacaaagg atgacctgct gatcgacgtg gcaaagaagg acggctatgt
     8161 aacggaagag gacaattatg tgaccgttgt cctggatacc aatctgactc ctgaactgat
     8221 tgaggagggc tttgtccgcg aggtgatcag caagatccag accatgcgta aggaagccgg
     8281 ttttgaagtt atggatcata tcgccgtata ccaggatgga aatgagcgca tcgcagagct
     8341 tgtaaaggca aatgcagatg agatcaaaac agaggttatg gcagaccata tccacttagg
     8401 ccagatgaag ggctttgtaa aagagtggaa catcaacggt gagagcgtaa tgcttggcgt
     8461 tgaaaagcta atgtagggag ataaaagagt ataaaaggcc gatccgccct gaaacgggag
     8521 ttttcgggcg gatctggaaa gtaagaagga gaatccgatg aatcctatgg aatttgacaa
     8581 ggccaccttt aaaaaatccg ttatgtacaa tgtgaaaaac atgttccgcc gcaccatcga
     8641 cgaggccact ccccagcagg tgtttcaggc ggttgccttt gcggtaaagg atgtaatcat
     8701 tgacgaatgg atcgcaacgc ataaggaata tgagaaaaag gatgcaaaga ccgtttatta
     8761 cctttccatg gagtttttaa tgggaagagc gcttggaaac aatattatca atatctgtgc
     8821 ccgtaaggag atcgcagagg ttcttgagga gctgggcttt gacttaaatg tgattgagga
     8881 tcaggaaccg gatgcagctc ttggaaacgg cggtctgggc cgtctggcag cctgcttcct
     8941 cgattccctg gcgaccctgg gatatccggc ctatggctgc ggcatccgct ataaatacgg
     9001 catgttcaag cagaagattg aaaacggcta tcaggtagag gttccggaca actggctgaa
     9061 aaacggcaat ccctttgaga tccgacgtcc ggagtacgcg gtagaggtaa aattcggcgg
     9121 ttatgtgcgg attgaaaaca gagacggcat gaaccacttt gtacaggacg gctatcagtc
     9181 cgtaagagcc gttccctatg acctgcctgt gatcggctac ggtaacaatg tagtcaatac
     9241 actgcggatc tgggatgcag agcctatcaa tacctttaat ctggattcct ttgaccgggg
     9301 cgactaccag aaggcagtgg agcaggaaaa tctggcaaag accattgttg aagtgcttta
     9361 tcccaatgac aaccactatg caggcaagga actgcgctta aagcagcagt atttcttcat
     9421 ctctgccagc gttcaaagag cggtattaaa gtataaggag cttcacggca ccatccatgg
     9481 attccaggat aaggtggtat tccagttaaa tgatacccat cctacggttg cgatccctga
     9541 gctgatgcgt atccttttgg atgaagaggg actgacctgg gaggaagcat gggatattac
     9601 cacaaagacc tgtgcctaca ccaatcatac cattatgtcc gaggctctgg agaagtggcc
     9661 cattgacctg ttctccaggc tgctcccccg tatctatcag atcgtggagg agatcaaccg
     9721 ccgctttgta aatgagatcc aggcccgcta tccgggagat caggagaaga tccgccagat
     9781 ggccgttgtc tatgacggac aggtgcgcat ggcctatatg gcaattgtgg caagcttttc
     9841 tgtcaacggc gttgcaaggc ttcatacaga gatcttaaag catcaggaat taaaggattt
     9901 ctatgaaatg atgccggaga agttcaacaa taagacaaac ggcattactc agaggcgctt
     9961 cctgcttcac ggcaacccgc ttctggcaga ctgggtaacc tccaagatcg gagaccagtg
    10021 gatcacagat ctgccccata ttgcagagct tcttccctgt gcagaggaca aggaatgcca
    10081 gaaggagttt atgcagatca agtacgagaa taaggtccgt cttgcaaagt acatcaagga
    10141 gcacaacggc attgacgtag atccaagatc gatcttcgat gtgcaggtaa agcgtctcca
    10201 tgagtacaag cgccagttga tgaatattct ccatgtaatg tatctgtata atcagttaaa
    10261 ggatcatccg gatatggata tggttcccag aacctttatc ttcggcgcaa aggcagcggc
    10321 aggctataag agagccaagc tgaccattaa gctgatcaac gcggttgcgg atgtggtgaa
    10381 caacgataag tccatcgggg gcaggatcaa ggtggtattt atcgaggact accgtgtatc
    10441 caatgcagag atcattttcg ccgcggctga tgtgagcgag cagatttcca cagccagcaa
    10501 ggaagcttcc ggtacgggca acatgaagtt tatgttaaat ggcgccctga ccctgggaac
    10561 aatggacggc gctaacgtgg agatcgtaga agaggttgga aatgaaaatg ccttcatttt
    10621 cggtatgtct tctgacgaag taattgccca tgaaaagaac cgcgattatc agccaatgga
    10681 tatctttaat aacgatccgg agatccgccg ggtactgatg cagcttgtaa acggctttta
    10741 ttcaccggaa aatccggagt tattcagaga tatttatgat tcactattaa atacaaagag
    10801 cagcgaccgt gcagacacat atttcatcct gaaggatttc cgttcctacg cagaggcaca
    10861 taagaagatc gatcaggcct acagaaattc ttcctggtgg gcaaaggcag caatcttaaa
    10921 tgtaggacac agcggaaagt ttacctctga tcgtaccatc gaggaatatg taagagatat
    10981 ctggcattta aagaagatta ctgtggaagt ataatgactg cagtctgaaa cagtaaatga
    11041 tccggctccg gcgtctgtaa cagggcgtcg gggtcttttt acaacggttt ttggtatgtg
    11101 atccgatccg ctgtatatca atcacaaggg aggctatgac atgaaaatcc gtgacatctt
    11161 agcagaggga aaaccaaccc tgtcatttga agtatttccg cccaaaactc aggatgcatt
    11221 tgcatcggtt gagaaagcgg cagaggaaat cgcaaggctg catccggcct ttatgagcgt
    11281 tacctacggc gcaggaggcg gcaccagcga ttatactgta aagatcgcgt ctgatctgca
    11341 ggaaaactgt ggggtaactc cgctggctca tctgacctgt gtttcttcta ccagagaaaa
    11401 ggtccagcag gtattgaacg aactgaagga gcataagatc gaaaatatcc ttgctttaag
    11461 aggtgacatt cctcaggatg gacagacgcc tcaggtatat cactatgcct ccgaactgat
    11521 ccgggagatc cgggaggcag gagatttctg tatcggagcc gcctgctacc ctgaaggcca
    11581 tgtggagtct gccaacaagt ctgtggatat ggattattta aagcagaagg tggaggccgg
    11641 ctgcgatttt gttaccaccc agatgttttt tgacaacagt attctctaca gctatctgta
    11701 ccggatccgg gaaaagggga ttaccgtacc tgtgatcgcg gggattatgc ctattacaaa
    11761 tgtaaagcag gtaaagcgca gcatccagat gtccggctgc catatgccgg cccgctttat
    11821 gtccctggtg gataagttcg gagatgatcc cgctgccatg aagcaggcag gaatcgccta
    11881 tgccacggat cagatcattg acctgatcgc aaacggcatc cgcggaatcc atgtatatac
    11941 catgaataag ccagatgtag cggcaaagat ccgggaaaat ctgtcagata ttgtaaaata
    12001 aaagattcca tgcagcagga ggcatataga tggacatttc ttccgtaagc aggagagagg
    12061 tgctgcgtta tctgggatac agggggcagg cagaaccgga tagggcagtt gcagatgaga
    12121 tagaaatgtg tctggggctt ttggcagaga aggcagagcc caggcatcgt gtccgctctt
    12181 ttttactgga gctgggcccg gagcatgaga ttcgttttga cagggttcat acaagcagca
    12241 gacagctttc taaaaatctg gcaggctgcg gtgaaattct tgtttttgcc gctacattgg
    12301 ggctggggac agattatctg atccagagat ataaccgtct gcagatgagc cgggcagtga
    12361 tcctgcaggc tgcggctgcg gccatgatcg aggaatactg tgaccgggtc tgcagggagt
    12421 taaaggcagc atatgaagca aaagggctgt atcttcggcc aagattcagt ccgggttacg
    12481 gggattttcc tctggaatgc cagccgtatc ttttagagtg cgtggatggg ggaaagcagc
    12541 tgggaatccg tcttacggga gggtatctga tgatgccgtc caaatctgtg acagcggtga
    12601 tggggcttgg aaggacgccc tgcggctgtg aggtaaaagg ctgtgaggcc tgtggaaaaa
    12661 aggactgcgc ctatcggcgg caaacaggaa gcgaggatta aggaatgaac attttacagg
    12721 aaatgagaca gcggcgcctg ttttttgacg gcggtatggg aagccttttg caggctaagg
    12781 gattaaaggc aggagaactg ccggagcggt ggaacctgac ccatccggag gaaatctgcg
    12841 ggatccaccg gatgtatctt caggcaggct ctgatcttat aaccacgaat acatttggag
    12901 ccaaccgctt aaaatacggc gaagaattgg aaaaaattgt aagtgaggcg gtaagccatg
    12961 caaaagaggc cgttagggca gagggacacg gctatgtggc cctggatctg ggtcctacgg
    13021 gaaagcttct taagcctctt ggcgatctgg agtttgaaga tgcggttgag atttataagg
    13081 aaaccgtatc atatggagcc agagcgggag ccgacctggt gctgatcgaa accatgagcg
    13141 attgctatga actgaaggct gctgtacttg cagcaaagga agcgggattt tctcctgaaa
    13201 caggggagcg gctgccgatc tttgcaacag tcatctttga tgaaaaggga aagcttctga
    13261 ccggcggcga tgtgtcctct accgtagctc ttttggaggg gctgggagtg gatgtgctgg
    13321 gaattaactg cggtctgggt ccggtacaga tgaagggaat cgtagaagag cttctttctg
    13381 tgtcatcaac cccggtgttg gtaaatccca atgcaggcct tccaagaagc gagaacggga
    13441 aaacggtcta tgatattgac gcagtccggt tcggcgaggt tatggaagaa attgtaaaaa
    13501 tgggagctgt gatcgtggga gggtgctgtg gaaccgggtc ggagcatatc cgggaaacgg
    13561 taaagcggtg taaggatctt cctgtgatgt ggccggaaaa aaagaaccgg accgtggttt
    13621 cctcctattc ccaggcagtg gtatttgaca aaaagaccgt aattatcggc gagcggatca
    13681 atcctacggg aaagtcaaaa tttaagcagg ccctcaggga tcataacctg gaatatatcc
    13741 tgcgggaggg cgtgacccag caggacaacg gcgcggacat tttggatgta aatgtagggc
    13801 tgccggagat tgacgagccc tctatgatgg aggcagtggt aaaggaactg caggcggtga
    13861 tcgatctgcc actgcagatt gacacctcca atccacaggc catggagcgg gccctgcgga
    13921 tttataacgg aaaggctttg atcaattctg taaacggaaa aaaagaggta atggatcaga
    13981 tctttccgct ggtggcaaag tacggcggag tggttgtggg actctgtctg gatgaagacg
    14041 gcataccgga gacggcagag ggaaggattg ctgtggggag aaagatcatc aagacagcgg
    14101 cctcttacgg catcggaccg gaggatatta tcctagacgg cctctgcatg acagtcagct
    14161 cggataacaa aggggcgctg actacgttag agactctgag acggatccgg gacgagctgg
    14221 gaggcaagtc tgttctgggc gtatccaata tctcctttgg cctgcctcag agagaaatca
    14281 taaacagctc attttttacc atggccatgg agtgcggctt aaacggagcg atcatcaacc
    14341 ccaattccga ggctatgatg cgggcctatt acagcttcaa tgccttaatg gaccgggatc
    14401 cccagtgcag ccggtatatt gacatttatg gcagtgaaac aggtgggctg ggacagaccc
    14461 tttcccggac aaaggatacg ggaaagggaa aagaaggcga ttctctggga atactgattg
    14521 aaaaaggcct gaaagaggct gcggcagaga cggtggcagc tcttttggag gaaaaggatg
    14581 ctctggatat tattaacggg gaaatgatcc cggctctgga tcgggttgga aaaggatttg
    14641 aaaagggcag tgtgttcctt ccccagcttt taatgagcgc tgaggcggca aaggcggcct
    14701 ttgaggtgat caaagctaaa atggaaggca gcggacaggg acaggagaaa aagggaacga
    14761 ttattctggc tacggtaaag ggagatatcc acgatatcgg aaaaaatatt gtcaaagtgc
    14821 tcctggaaaa ttacagctac gaggttattg atctgggaaa ggatgtgccg ccggagagaa
    14881 ttgtagagga agcagtgaaa cgccatgttc ctctggtagg tttaagcgcc ctgatgacaa
    14941 ccacggttcc cagtatggag gaaaccatcc gccggctgag aaaggaagca tcctgggtaa
    15001 aggtaatggt gggaggcgca gtgcttacaa aggagtatgc ggataccatt ggagcagatg
    15061 cctactgtcg tgatgccatg gcttcggtcc actatgcgga aacagtaatc ggttgagaga
    15121 tatcagaaag gaatcgtatg ggacataaca caagggaaaa cacaaaaatc cttttaaagg
    15181 gcgcctgggt aggaggtacc atgaccgtac cgggagtcag cggaggtact atggcaatgg
    15241 tgctgggaat ctatgaccgc cttatttcct cggtgagccg tgtgtttaag gagccgaaac
    15301 aatctattcc ctttttactg tggtttgcac tgggaggagc ggggggtatc ctgctctttt
    15361 ccagaggcat cagcctgctt ttatcctccc ggtgggaggt tcctgcccgc tttttcttcc
    15421 tgggagctgt cggcggaggg attcccctga tttttaaaga agcgggaatt aaaagatttg
    15481 gctccgggga cgctgttttt gtgggaatcg gtatcttaag cgctctgctg atcggcctga
    15541 tcccggaagg cctgttcacg ccgggagcag gaggggtatc cgatattctc atacaggctt
    15601 taggaggagt tatcatcgca gtggccctgg tccttcccgg gatcagtgcc tcccagatgc
    15661 tctatatgct gggaatctat gaaagcactt taaaagccgt gggaaatatg gatattgccg
    15721 ccttggttcc tctggcggca ggcgggcttt tgggcacctt tttttctgca aaagtgttgg
    15781 aagggctgtt ggaaaaacat acccggccca cctttcttct tattcttggc tttatgtttg
    15841 cttctctgcc ggagctgttt ccgggaattc cggcaggagc agaggccgtg tacagcctgg
    15901 tggctgtggg agcgggattt atgctgcttt atgggattgg gagaatgaat tagctatagc
    15961 catcccccat tttctgtgat atgatatacc cctgataagc ctgtttcggg cggacaattg
    16021 aaaaggaagg aagacggtta tgtataaatg ttgtatgttt gatctggacg gtacgcttgt
    16081 aaattccatt tatgctttaa agcggtctgt ggacggaaca ctggcgcatt ttggcctggg
    16141 gccgatcacc ttggaagata caaagacatt tgtaggagac gggtataaaa aactggtgga
    16201 acgggcttta aaggcgtacg gagatccgga actggttcat tttgaagagg cgctgaacgt
    16261 gtataaccgg gtatttaagg aatgttgcct ttaccgggta gaggcatacg agggaatccc
    16321 ggagcttctg gcatttttaa aggaaaatca cattctctgt accgttttat ccaataagcc
    16381 tcatgtaaga accatagaga atataaacta tgtattcggg ccggatgttt ttgacagggt
    16441 atatggggaa cgggaatctg cggggatcaa aaaaaaaccg tctccagagg gggtagaagc
    16501 gctgcttgag gagcttggga tttctaaaga ggagtgcctc tatctgggag atactaatac
    16561 agacatggaa acaggaaaaa attctcaggt agatacggtg ggcgtactct ggggctttcg
    16621 ttccagggat gagcttaaag tgtatgagcc tgcgctttta gcggagcatc ccatggatgt
    16681 agcggaattt ataaagggcg tgaatcacat tgaatagaca gaaaaaaaga agtggagcat
    16741 ggacgaaaac aggccttctg aaaacaggta tcctgttttt cgccctgctt ttgggcagcc
    16801 ttgcatttac aggctgtgcg aaaagagaat ctccgggaat ccggggagag cagggcgggg
    16861 atgaccgcct ttttgtagtc actaccattt tcccctacta tgattttgtg cggcagatcg
    16921 gaggagaccg ggtgcggtta aatctggtcg taccggccgg gatggacagc cattcctttg
    16981 aacctacgcc cgcagatatg attaccattc agaaagcgga tctgatgatc tgcaatggag
    17041 gagccatgga gcagtgggtg gatcaggtcg ctgcatccct tgacaccagc cgtctgcgcc
    17101 gtctgactat gatggactat gtagatgtgg tagaagaaga ggttgtggaa ggaatggaga
    17161 aagaggagca cggtcatgaa cacagctgtg aggaggaaga ggctggggaa tttgatgccc
    17221 atgacgggca ggagcttgat attgaatacg atgaacatat ctggacttct cctgtgaatt
    17281 ccatgaagat tgtggagatt atttctgata ccctggcgga aatggatcct gcaaacgaag
    17341 cctattacag ggaaaatgcc gcctcatatc tggaagaact ttctgcactg gatgagcagt
    17401 tcagggagat tgtatcaaat ggccgccacc ggatgatggt gctggcggat aaatttcctc
    17461 ttcggtattt tgcggatacg tatggtttgt cctaccgggc tgcattttcc ggctgcagta
    17521 ccgatacaga gccaagcgcc agaaccatcg catatcttat tgaccgggtg cgggaaaatg
    17581 atctgaaagc cgtatactat ctggagcttt ccagtcaccg gacagcagat atcatcagtg
    17641 aagaaacggg gacagagccg cttttgtttc attcctgcca caatgtaagc cgcagggaat
    17701 ttgacgaggg cgtgacctat ttacagctta tgcggcagaa tgcagagcac ttgaagaagg
    17761 gatttgaata ggaaatgata gaaccattga ttaaatgtga gcatgtggat ttcggatatg
    17821 aaaaccagga tgcggtggta gatgtaaata tggaggtatg ccccggagac tatctctgta
    17881 ttgtaggtga aaacggatcg ggaaaaagta cgctgatgaa aggtctttta ggccttttaa
    17941 agccctccgc aggggtactt acggtatcag aggaattaaa gcacacggga atcggctatc
    18001 tgccccagca gacggccgcc cagaaggatt tcccggcttc cgtacaggag gttgtcttaa
    18061 gcggctgctt aaaccgcagg ggacgcaggc ccttttattc ccgtacagaa aaggcggttg
    18121 cggcagaaaa tatggaacgc cttgggatta cgcctgtggc aaagcagtgc taccgggagc
    18181 tttccggcgg gcagcagcag agagttctga ttgcaagggc gttgtgtgct accacccggc
    18241 ttttgatcct ggacgagcct attacgggac tggatcccat ggcgatccag gacttttatg
    18301 ccatgataag aaggttaaac cgcgaggatg gggtggcgat ccttatggta tcccatgatc
    18361 tcagaaatgc agtggaggag gccaataaga ttctccatct gcaaaagcag gtgctttttt
    18421 acggccctgc ccacgattat atgaacagtc aggctgcggg ccatttcttc catgaaaagg
    18481 agcaggggca gtgtatccct acggcagtct gccggaaagg aggctgctgc catgaacata
    18541 ctaagtgaaa tgctgtcata tccctttctg gtacgcgccc tggtgggagg tatgttggtt
    18601 tctctgtgcg cgtccctttt aggcgtaagc cttgtattaa aacgctattc catgatcggg
    18661 gacggactgt cccacgtttc ctttggagca ttgtccatcg ccctggcaat gggctggtcg
    18721 ccccttaagg tgtctatccc ggttgtagtg ctggcggcct ttttccttct tcggatcacg
    18781 gaaaacagcc ggatcaaaag cgatgctgcc atcgccatga tttcagccag cgccctggca
    18841 atcggtatta tcgttacctc ccttactaca ggaatgacca cagatgtaag cagctatatg
    18901 ttcggaagca ttttagccat gacaaaggag gatatggctc tggctgtagt actgagcctt
    18961 gtggtgctgg gtctttttgt gatctgctac aatcagatgt ttgcagtgac ctttgatgaa
    19021 aattttgcaa gggctacagg cgtcaacgtg ggattttaca atatggtgat ttctgtcctt
    19081 acggcggtta ctattgtgct gggaatgcgg atgatgggag ccatgctgat ttccagcctg
    19141 gtgattttcc cctgtcttac ctccatgcgc gtatttaaaa gcttcggagg agtgatgatc
    19201 tcttccgggc ttttatccct tgtgtgtttc cttttgggaa tgatggcatc ctaccagttt
    19261 tccacacctg ccggagccag cgtggtggtg gtaaatctgg cggcctttgt gatctttggc
    19321 gcatggcaga tgcttgggag gagaaaataa gggtaacagt tcagccatgc gaagattcag
    19381 gcagaaaaag cgttgaaagc ttgctttcat aagaactttt ctgtctgaat cttcataggg
    19441 cggatgcccg atgcttcttg tccgctgtaa gcgggcaaga agatgcaatg ctgaactgtt
    19501 acaaaaagga aaaatggcag cagctgtctt gacattccca gagggatgtg ccacaatatt
    19561 tagttggtaa gactgtgact gcagtagaaa gaaggaagaa attaaaatga caaagattga
    19621 tattatctcc ggtttccttg gagccggaaa gactacattt attaagaagc ttttgcagga
    19681 agccatttcc ggcgaacagg ttgtgctgat cgaaaatgag ttcggagaga tcggaatcga
    19741 tggcggattt ttaaaggatt ctggcattga gatccgtgag atgaattccg gctgtatctg
    19801 ctgttccctg gtgggagatt ttggaaaatc tctggcagag gtgctgacga cctataagcc
    19861 ggaccgtatc atcatcgagc cctccggcgt aggaaagctg tctgatgtta tgaaagctgt
    19921 aattgacgta tctgcagata tggatgtggc cttaaacagc gcggttacca ttgtggatgc
    19981 ggcaaagtgc aaaatgtata tgaaaaactt cggtgaattt ttcaataatc agattgaaaa
    20041 tgcaggtacc attgttttga gccgtactga tatcacagac ccggccaaga tccagaagga
    20101 tgtggagcag attcgtgaga aaaatccaaa ggcagccgtt gtgacaactc ctctgggaga
    20161 gctgggcgga gaccagttat tagaaattat tgagaagcgc gataccatgc tggatgatct
    20221 gatggcagag gtgagagagc atcaccatca tgatgaggag tgcggctgcg gtgagcatca
    20281 ccatgatcac gagggccatg atcaccatca cggccatgaa catggcgagt cctgtacctg
    20341 cggctgtcat gagcatgagg agcatgagca ccatcatcat catgaccacg gcgaagactg
    20401 cacctgcggc tgccacgatc atgagcatca ccaccatcat catgcagatg aagtatttac
    20461 cagctggggc atggaaacta ttgttcctgt taccagggat cagctggagg acatcttaaa
    20521 gcgtctggca gatacaaagg aatttggcga tgtactgcgg gcaaagggaa tgcttcctac
    20581 ggaaaatccg ggagagtggc tgtactttga tctggttccc cagcagtatg agatccgcca
    20641 gggccgtccc gattataccg gtaaggtatg tgtcatcgga gccagcttaa aggaagaaga
    20701 attgaacagc ctgtttggaa gaggctaaag aggttgatat gatgggaaat gaaattgaag
    20761 ataatgtaat gcccctcttt ttgatcaatg gatttctgga agcgggaaaa acccagtttt
    20821 tggaatttac catggagcag gactattttc agacagaagg aaaaacactg ctgattgtct
    20881 gcgaagaggg agatacggag tatgacgaga aaaagcttaa gaaaaatcgg acagctgtgg
    20941 tttatgtgga tgatttttca aagcttaccc cggaatatct caacgaatta aaagtaattt
    21001 accgtccgga acgggtgatt atggagtgga acggcatgtg gaatcaggac gagttaaagc
    21061 ttcctgagga ctggttcatc taccagcaga t
//
LOCUS       DBMDUH010000030        20477 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_1612_length_20477_cov_8.078916, whole genome shotgun sequence.
ACCESSION   DBMDUH010000030 DBMDUH010000000
VERSION     DBMDUH010000030.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 20477)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 20477)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20477
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1612_length_20477_cov_8.078916"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="ACZPCG_04235"
     CDS             <1..183
                     /locus_tag="ACZPCG_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HIR1284318.1"
                     /translation="SCEDVILDLRKQGVVLGKKGKADVAEESRFAYKDIDQVMNAQTD
                     LVTPVKRLKTVGVVKG"
     gene            411..587
                     /locus_tag="ACZPCG_04240"
     CDS             411..587
                     /locus_tag="ACZPCG_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284319.1"
                     /translation="MSQEHGFAVKRLQGHRKNALFASAAGSIAAVSGKACAVSDTAQR
                     SRDKEVLESFALWA"
     gene            675..>1152
                     /locus_tag="ACZPCG_04245"
     CDS             675..>1152
                     /locus_tag="ACZPCG_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007719343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HIR1284320.1"
                     /translation="MIKKYKINENECGYLIRRGRFEKLLTVGTYYFLSLSGCEVKIVP
                     MTGAVNTYGIPEEILMKNPEFASRVVKNVLPDTSIAIRFVNGAFREVVCKPETLFWNV
                     FEKNEFQVIDITKPWMEETLPRMYMELMPAKYYKKIVIKDGETGLLYFDNQFERQLE"
     assembly_gap    1153..1252
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1253..1722
                     /locus_tag="ACZPCG_04250"
     CDS             <1253..1722
                     /locus_tag="ACZPCG_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008397871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HIR1284321.1"
                     /translation="ENVGTQLYTHAQLILREYVGRFRLDELLEQKEEISQYVLEEMKK
                     KQEDYCVEIVSAGIKDIILPGEIREIMNTVLLAEKRAQANVITRREEVASTRSLLNTA
                     RLMDENQTLFKLKEMEYLERICDKVGTISINGGKGVMEQLAELVGMEGKRAGR"
     gene            complement(1738..3231)
                     /gene="arfA"
                     /locus_tag="ACZPCG_04255"
     CDS             complement(1738..3231)
                     /gene="arfA"
                     /locus_tag="ACZPCG_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002600181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinosylfuranosidase ArfA"
                     /protein_id="HIR1284322.1"
                     /translation="MKASLCIDRHFTIGSVDKRIFGSFIEHLGRAVYGGIYDPGNPLS
                     DERGFRKDVFELIRQIDVPVVRYPGGNFVSAYHWEDGVGPRSLRPKRLEPAWTAVESN
                     EFGLNEFMDWTKAANTTPMMAVNLGTRGAEDAKNLLEYCNISGGTLYSDLRRSHGYQD
                     PHNIRLWCLGNEMDGPWQMGHKTAGEYGRLAAETGRLMKMVDPDIELVACGSSNRNMS
                     TFGYWEDQVLEQCYDQVDYLSLHTYYGNPQDNTPDFLACSADMDAFISSVISICDSVK
                     ARKRSKKQICLSFDEWNVWYHSKEQDKQLPRWVQCPPQLEDIYNFEDALLVGSMLITL
                     LRHADRVKIACLAQLVNVIAPIMTDSTGAWAQTIFYPYMHASCLGRGTVLQTQIQSPC
                     YESRYGDVPYLDAVTIENEEEETLTIFAVNKNLSEDIELTADFRQYEGYHVSRHIVMT
                     HSDLKAVNTKKNPNEVIPADTGISSLENGILSSHLPSKSWNVIILKK"
     gene            complement(3234..6212)
                     /locus_tag="ACZPCG_04260"
     CDS             complement(3234..6212)
                     /locus_tag="ACZPCG_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HIR1284323.1"
                     /translation="MAFVEVIRRSMRPEEWTDLRYSWIKRSLIKEKYEITDLEIRDAF
                     QTAENEYTYTSDYRPLKTGDMYFSPDGTAFIRGHAQIPESLKEKDIWFTLHTAAEMIV
                     KINGHYAGGIDPNRDRILVNPWLNKDSAELTIEIEGYNRSKPDDERNPDALSCRGCRQ
                     IFEGAYLAELNEPLLSLFYDLTILLDIAKSNHFDEDYRVFLNRELNNALNLVDFDTWE
                     GVEAALSYIETHIYENTDFRSSGNVALVGHSHLDIAYYWRRIHVVHKNARTILIQMRL
                     MDQYPDFKYAHTQPYTYELLSQYYPELFEELKEKVRSGQFEPVGAMYVEPDCNIPTAE
                     SLIRQCLYGQLYYREAFGITVDNAWLPDVFGNSWILPQILKKSGVDYFVSNKMSTWND
                     TNRFPHNNFLWKGIDGTRICACVPPTHFITWNMPSQIQENWDAYQDKETGGQTLSMFG
                     YGDGGSGVTEEMVELMHRFDKLSIMPKTEHMRADEFLHRNLKDNDQLETWDGELYLEM
                     HRGTFTTKAVLKDFNRRLEAKLRAAELLSVLRMKQGKEYPAAELRDCYKKLLLNQFHD
                     ILPGSHINPVFCDAVRDYEDLEKRLDAIINDSDSVFFNPLNKKRREASFIPSENGAFV
                     RRGIRGYFTYTHAEPLSSVSVSPCAGKENWFESSSCGTGLTVKTPFYDVQFEADGSIS
                     SLFDKKMNREWANGGFNKLHLYQDTPGMYDAWDILPNYKDVEYDFTAESPLSLQAFDG
                     ETAEFACVLRTPGNKSHWMVVIRLFRSSPAIEVEHIVDWDEKHKLVKAEFSCNVLSRE
                     LICDTSAGYIKRETHTNTSWQKARFEVCHHKWCDFAEESGGIALINENKYGVGIEENG
                     MSLSLLRANIRPDIQSDIGHHDFCYVILPHEGRAVTAGINDAALEYNHPLVKAEAAAP
                     DWDFGELYLQAVKLSENGEYIVFRLSEQNGRRGCLNLPQTMAVMNMLEDVEGETQTLY
                     YSPFELITLGLPVKDYLRGK"
     gene            complement(6237..6470)
                     /locus_tag="ACZPCG_04265"
     CDS             complement(6237..6470)
                     /locus_tag="ACZPCG_04265"
                     /inference="COORDINATES: protein motif:HMM:NF013286.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HIR1284324.1"
                     /translation="MEEYICNTIGPRIRGDGGWVEFVSYEDRCLTLIFRGECSKCIIL
                     NRCLDWIRQEILRDLGEDVTIKPICKKPFFWDH"
     gene            complement(6586..8391)
                     /locus_tag="ACZPCG_04270"
     CDS             complement(6586..8391)
                     /locus_tag="ACZPCG_04270"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1284325.1"
                     /translation="MKKQLPRHFRDASLMGKINIVIGILTLVLTTLITAISIFYYSFI
                     LSDRQFERTKQAAQNASETLSYNYKNIMDRLTAICGTAEFSSDIRMLASPSVSYIVQE
                     RLVQNELSDLAGCNYLVQSALVLSGDGKTAYSLYKNPLPDTAEFFTEEELYGLHGVTC
                     LPIRQSPLRSRVPVLPLVVPIRVNEAEYAVVNITDEPSDVYVVVYLDCTKLLQSLTLA
                     STGLAEGTYYLFTEDGVPLSPSSYDKKTNPLMLPETDALIRTLTETDETHTAAQTSDF
                     YLAASHMAKSGLILLECVPKEPFYDIFGPTIDFLVIVLFLVVGILLVISRMMTRYITR
                     PVTQLMAIVERIRENRYEHKQELGTGDEIGHLCTAINQMHDTIQKQMERIKQEESEKY
                     MAEIRLLTEQINPHFLYNTLDCIQSEVKRGESDTAANMIQYLAEYLRIGLSNGADLIP
                     LSSEIRHANAYVRLMNQRFGQSIRFMYHLAPGLSHHLIVKTILQPLVENSIKHGFGVD
                     SPGIPISTPAIEVIITAEEDELVININDNGSGFEPSELDELLKKSSTAKGGHVGILNV
                     YQRLTAYYGAEKVYISFSSIPYYCNVISIRIPLGS"
     gene            complement(8412..10013)
                     /locus_tag="ACZPCG_04275"
     CDS             complement(8412..10013)
                     /locus_tag="ACZPCG_04275"
                     /inference="COORDINATES: protein motif:HMM:NF012301.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1284326.1"
                     /translation="MEQNTVEFTAVLADDEYSVLEGVKTAIDWESLGIRIAALASNGH
                     EALDAIIKYKPDLAIIDIRMPDLTGIQVIERCRNAGISTDFIILSGYDDFSYAKEAMR
                     YGAKAYLLKPLNSTELTDEICRIFMERTSQSRHSTRRLYQEQFNFNFFNNLIDGKILE
                     SGIISRMLCDTQLPLSDSSCYVCAFIFEETLSSGTASFSPDPVLKQLNQEFAGEKCIF
                     WKHDAKQLIGIFNTSSLIPFHVALRCLKSLKNAGLSLPLIGVGDTVSGLMECSYSYNR
                     ALTALTYQLYDSSSHIFTYETICSVPPKTRLSDIDYLPLFQYIVKKDRDKIKAYCDEF
                     IDSLLYVPMPPPNYVYSLCYALFHQVQQEFSNFSHGEISEIATARDLYRFKQLSQIRQ
                     WLCDSFCQLSEFIDAVYGYASSKYAQVQENASKADDPIICTAKEYIHSNITNHIKIED
                     IAREVHLSPSYFAIYFKNKTNVNLRDYLLTEKMEYARRVLMNPSASISDVAYDVGYGD
                     YRSFSRAFKNVHGITPSDFQAKYRS"
     gene            10260..11666
                     /locus_tag="ACZPCG_04280"
     CDS             10260..11666
                     /locus_tag="ACZPCG_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1284327.1"
                     /translation="MRKKRFISIALAAVMAASVVGCGSGSDTAANVENTQAEGGEAKA
                     PEGATTVEVWTEDRHDLEYVEKMIDQYNQNNDDNIFINLTVITEDYKNMLALAYNGGT
                     APDVVGANSLPLNTFADTGILMPLNEYIDSDETFQKVNEPYEHAYEGENTRNGNIYFV
                     YSGMRSGVRVEYNKNILEECGYTEVPSKLEDYIDMAKDITEKGAGNYYGIGFTSASTF
                     ERLLEMSAQVSGIYYYDYVNGKYDFSGYKDIVELGKRFISEDIAYPDQQGVDNMRALF
                     AEGQFALWSNASQEASVFTNQIPITEFEWGVAEVPSLTGEIEGALQTTPSKAYGIVST
                     SEHKDEAWKVIQYFQSEEFLKGYLESGHCLPISTYMDAAIDKTKIGRMADFSLLDYES
                     VYPKPPVINLTGDDYRVVLWNAVMDYVDVDEAIEDLNTRYNEALEADVASGATKRLVI
                     SDYDPLHPSDGTITYLDK"
     gene            11750..12664
                     /locus_tag="ACZPCG_04285"
     CDS             11750..12664
                     /locus_tag="ACZPCG_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1284328.1"
                     /translation="MNTKKKKFKITRDGIAAALMLTPSTILLAVCSIYPFIWIFRYVC
                     FDYNGFTATYTGSRNFMRMLSDKAFWGSVGHTMEYAVYKLAFIIPLALLLAVLLNAKM
                     RGSNFFRGIYFMPTVISTSISGMIFAFIFATQNGIANEMLLSAHLIDSPIKWLTSKEW
                     VMVAVTVLAVWGGLGNYMLYFTTGMNSVAEDVYESAKIDGAGAVQTFFYITLPMLAPV
                     LKVVLMLAITSAFKDYEAIMVLSKGGPGNRSMVMFLYIYNLIFGTTNTTTQAQIGYGA
                     LLSVVAALIVGIITAIYLFVSRKLDDVV"
     gene            12676..13542
                     /locus_tag="ACZPCG_04290"
     CDS             12676..13542
                     /locus_tag="ACZPCG_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1284329.1"
                     /translation="MEKGHAVYSTKSKILRGIVWVLMLILALFMVFPILYIILGSFKE
                     NSELLVGGTNILPSHWVFSNYSDAWKQANFATYTMNSIFLALGVMTITLLTSTMSGYV
                     FSRRNFRGKELLYSLFVAFMFINVGSVSLRPLFELAVKLHMNKNLFSVILISVGTGQA
                     TYIFLCRGFVNSLPKELDEAAKIDGCTFFQTYRLVIVPLLKPVMATIALLSFRQGWNE
                     YILPLVFTMTNDKLRPLTVGVNMLKNAGDGAAAWNIMFAGATIAIIPMLITYCIFSRY
                     FMGGMTTGAVKG"
     gene            13620..16058
                     /locus_tag="ACZPCG_04295"
     CDS             13620..16058
                     /locus_tag="ACZPCG_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284330.1"
                     /translation="MKEWMLGHDGFIMEYMIAGPKVTPFKSDERAENQLELEARLRAQ
                     IVTPKKEEYQVDPRLGQEAENGCRWSVWAPGNNCFIDVSHFYSTLQSVSLLAAVNLNA
                     DTACEVQARIWTYMAVGIYCNGKLAGEVKRPVYKPIQYQDVIFQLNQGKNLILCECEN
                     LGVRDTRNIVGIQIVSHREHIKTALPDDRFQEQVFEDTEFMRQLCLEQGSLVMPEIAG
                     AETSVCFHRDSPDYEVMCLPQKEISLEGKKILPVPEETALISVIRKRPEYELSRELEF
                     TERRLPCYPSSGMTEEENWKRILKEIASVGSLNRGKFGFAVFNILARKQLGIHDPKDR
                     QRLFETLDLIEERVDCSDFLVCGLIRYMHLYEMDEKLQERTKQVLLNYRYWMDMDGAD
                     AMCFWSENHSLMFYSAAMEAGKLYPEEYFPRAGMKGEALQKWGRSRLLQWLDDTEEYG
                     FEEFLSTVYMCVTFAALLNVIDFAEKEISDRARALEDHLVDELCTQTFKGSIIAPMGR
                     VYREVIYPFCQGAQSLINRIDPEAPWAYGEGWLGFLSGSTYRFPQGMKERMKQDISCR
                     CTSGNAAIVLEKTQDYCLTSVESPRADRDARWENIRSGQGEKVHTHAFTKSLNECFHG
                     TSAFGPGIYGYQQHMWYGALSPEAVIFANHPGATSEQSDMRPGYWYGNGVMPAVKQVP
                     GKVGAVYRIPESHPIHFTHIYCPRDRFDRVLKAGNWLLLKKDTGYMGLWCSTELTPFD
                     DTIFHCELRAYGDDMAYLCVCGSTEKWNTLEEFGNWVQSLKPSYCRETGTLKTEDGFE
                     LHYQAVQDDTQYLL"
     gene            16190..18052
                     /locus_tag="ACZPCG_04300"
     CDS             16190..18052
                     /locus_tag="ACZPCG_04300"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012516.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HIR1284331.1"
                     /translation="MNRHVSDGDHTETVQVASSSRDLTYWTVPSVFEGGQPEGEAGKE
                     AEPSRSWTQFPWAAAGIHAVSAVWDTELEMYLAVYEGQETTGKLSGSIGLAVSKDLIH
                     WQSFFRDQPTYPGHGVIYEGDYYPSIPFGEYIYSMELPAGENVFDVRDYGAVPDGETL
                     STEAVQAAVDAAAACGGTVLVAGGYYCVGTVYVASGVTLWIQKDSALCASRDLKQYKD
                     ALIACVNAENVKITGGGKIIGRGEYFVYLPKNPPLTVPMETTKLPPFLYDPMGYPVDS
                     IRYTYRSRIRYAEDRYAEGLPSIPRPMYTLWVRGCTNAVIENIIIEDALDWTLSIDCS
                     HQVMVHDLVINGNRHVANTDGIDIMGSSQVEIDHCFVSCADDGICVKSPMKQGHDGIT
                     IEDADAEMKGTEQVHIKNCTVVSVMNAFKIGTETYFDIKDITVENCRFMLPDIYPGTV
                     SGISIESADGSRIQNVQIRNIEMDRVCCPVFICLNMRNKYGFLNETDRQEKAFGGAIE
                     HVVIEHVRAYNTEVPSIITGFQTDENGVHTERRVSDIRIRDFKAVYHDNKEVLDIRTP
                     VYENVRDYPENNAFGDVPAYGFYIRHADRVTLEDWEVIPRTMNTREALVREYTD"
     gene            18067..19830
                     /locus_tag="ACZPCG_04305"
     CDS             18067..19830
                     /locus_tag="ACZPCG_04305"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006770899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HIR1284332.1"
                     /translation="MSTVYETIKNEYEVWKKKVTEDADLTAELTDMEGKEDRITDAFY
                     QNLAFGTGGLRGVIGAGTNRMNIYTVGKASQGLADYINRHFPKEERKIAISYDSRIKS
                     SLFAKRAAEVFAANGIRVFIYTELMPTPMLSWAVRSLSCAAGVMVTASHNPSKYNGYK
                     VYGPDGCQITTDAAAEILGCIEKTDAFEDVKREDFETALENGRISYIGEDTVTSFIEA
                     VKGQSMLTKSEENVDKNISIVYSPLNGTGLKPVLRTLKESGFTNITVVKEQEQPDGHF
                     PTCPYPNPEIREAMALGMEYAKKCSADLLLATDPDCDRVGIAVPNREGEYVLLSGNET
                     GLLLLNYICSMRQAAGTMPEHPVFVKTIVTMDMAARIAADYGVKTVDVLTGFKFIGDQ
                     IARLEAEGRENDYILGFEESYGYLSGSYVRDKDAVDGAFLICEMAAYYRSKGMGLLEV
                     LEGLYEKYGFCLNTLHSYEFEGMEGFEKMKGIMNRLHTMASRIAEGEAVCIGGRKTEA
                     VEDYLSGTISRADKTTEPIAGLPSSDVLKFRLAGNASVVVRPSGTEPKLKLYISVSAE
                     DKAKAQAEEEKITAQMEEWFL"
     gene            19987..>20477
                     /locus_tag="ACZPCG_04310"
     CDS             19987..>20477
                     /locus_tag="ACZPCG_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HIR1284333.1"
                     /translation="MSYVDEIYERVVAQNPGEPEFHQAVKEVLDSLKLVIDANEEKYR
                     KAGILERFTEPERIISFRVPWVDDQGNVQVNKGYRVQFNSAIGPYKGGLRFHPSVNQS
                     ILKFLGFEQTLKNSLTGLPMGGGKGGSNFDPKGKSDREIMAFCQSFMTELYRHIGKDT
                     DVPA"
BASE COUNT         5507 a         4338 c         5362 g         5170 t
ORIGIN      
        1 tcctgtgagg atgtaatcct ggatctgaga aaacagggcg tggttctggg aaagaaggga
       61 aaggcagatg tggcagagga aagccggttt gcctataaag atatcgatca ggtaatgaac
      121 gcccaaacgg atctggtaac cccggtcaaa cggctgaaga ccgtaggggt ggtaaaggga
      181 taatgatgga agagtccggc acctgcaggg caggagggcc ggtgtcagat acaggcagtt
      241 ctgcccatgg gaggagtatt tcctggctcc ccggtgccgt cgcctatggc gttttggtat
      301 tccggcagga cagcatatag tcagcacgat gggccgcagg ttcaaatcct gccatgatgg
      361 caagtcatgt agctaagcgg gtatagcaat cgttaagaga catcttaaaa atgtcacagg
      421 agcacggctt tgcagtcaaa aggctgcagg gccacagaaa gaatgcgctt tttgcttcgg
      481 ctgcaggcag catcgcggca gtctccggca aggcctgtgc agtttcggat accgcccaaa
      541 ggagccggga taaggaagtt ttggagtcct ttgccctttg ggcttaagaa acagccccct
      601 gggccgggga cgaagcatgg cgcaggattt tctgtatgaa acagggaaat tgtttgtgaa
      661 acagggaggt acctatgata aagaaatata agatcaatga aaatgagtgc ggctatctga
      721 tccgcagggg ccgctttgag aagctgttga ctgtgggaac ctactatttt ctcagcctgt
      781 ccggctgtga ggtaaaaatt gttcccatga ccggggctgt aaatacctat gggattccgg
      841 aggaaatcct gatgaagaat ccggaatttg cttcccgtgt ggtaaaaaat gtgcttccgg
      901 atacatccat tgccatccgg tttgtaaatg gagcgttcag agaggtggtc tgtaagccgg
      961 agacattgtt ctggaatgta tttgagaaaa atgaatttca ggtgattgat atcacaaagc
     1021 catggatgga agaaaccctt cccaggatgt atatggagct gatgcctgcc aaatactata
     1081 agaaaattgt gattaaggac ggagagaccg ggcttttgta ctttgacaat cagtttgagc
     1141 ggcagctgga aannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntggaaaat
     1261 gtgggaacac agctctatac ccatgcgcag ctgatcctca gggaatatgt aggacggttc
     1321 cgtctggatg agcttttgga gcagaaggag gagatcagcc agtatgtgct ggaggagatg
     1381 aaaaagaagc aggaggatta ctgcgtggaa atcgtctctg cgggaatcaa ggatattatc
     1441 cttccggggg agatacggga gatcatgaat actgtgcttc tggcggaaaa acgcgcccag
     1501 gccaatgtta tcacccgaag ggaggaggta gcttctacca gaagcctctt aaataccgca
     1561 aggctgatgg atgaaaacca gaccctcttt aagcttaaag aaatggaata tctggagagg
     1621 atttgcgata aagtggggac gatttctatt aatggcggaa aaggcgtgat ggaacagctg
     1681 gcggagctgg tggggatgga aggaaaaaga gccgggagat agattcccgg ccttgtctta
     1741 tttctttaaa ataattacat tccagctttt ggaaggaaga tgagaggaca gaataccatt
     1801 ttccagagag gaaatgccgg tatctgctgg aatgacctca ttgggatttt tctttgtatt
     1861 aactgccttt agatcagaat gggtcattac aatatggcgg cttacatgat agccctcgta
     1921 ctgtctgaaa tcagccgtca gctctatatc ctcagacaga tttttattga cggcgaagat
     1981 cgtaagtgtt tcttcttcct cattttcaat agttacggca tccagatagg ggacatcgcc
     2041 gtaccggctc tcatagcagg gagactggat ctgggtctgc agtacggttc cccgtcccag
     2101 acagctggca tgcatatagg gatagaagat cgtctgagcc catgcgcctg ttgaatcggt
     2161 cataatgggg gcaattacat tgaccagctg agcaagacag gcgattttta cccggtctgc
     2221 atggcgcagt aaagtgataa gcatgcttcc cactaaaaga gcgtcctcga aattgtaaat
     2281 atcttccaac tgaggcgggc actgaaccca ccggggaagc tgcttatcct gctccttgga
     2341 gtgataccat acattccatt cgtcaaagga aaggcagatc tgctttttgg aacgttttct
     2401 ggctttgaca gagtcgcaga tggaaataac ggatgagata aatgcatcca tatcagcaga
     2461 gcaggctaag aagtcaggtg tgttatcctg aggattccca taataggtgt gaagggaaag
     2521 gtaatccacc tgatcgtagc actgttccag cacctgatct tcccagtagc caaaggtgga
     2581 catgttccgg ttggagcttc cgcaggctac cagctcaata tcaggatcta ccattttcat
     2641 caaacggcct gtctcggcag ccagccgtcc gtattcaccg gctgttttgt ggcccatctg
     2701 ccaggggccg tccatctcgt ttccaaggca ccagaggcgg atgttatggg ggtcttgata
     2761 gccgtgggaa cgccgtaagt cactgtaaag agtaccgccg gaaatattgc agtattccag
     2821 cagattttta gcgtcctcag cgccccgtgt gccaagattg acggccatca taggagtcgt
     2881 gttggcagct tttgtccagt ccataaattc atttaagccg aattcattgg attcaactgc
     2941 tgtccaggcc ggttccagac gtttgggacg cagggaacgg gggccaaccc cgtcctccca
     3001 gtggtaggcg gaaacaaagt ttcctcctgg ataacgcacc accggcacat caatctgacg
     3061 gatcagctca aaaacatctt tgcggaatcc cctttcatcc gaaaggggat tcccaggatc
     3121 atagataccg ccgtaaacgg cgcgtcctag atgctcgata aaggaaccga agatgcgttt
     3181 gtcaacagag ccaatagtaa aatgtctgtc aatgcacaaa gaagccttca taattatttt
     3241 cctctcaggt aatccttcac aggaagtccc agcgtgatca gttcaaaggg gctgtagtac
     3301 agagtctgtg tttcaccttc tacatcctcc agcatattca ttacagccat tgtctgaggc
     3361 agatttaagc agccccggcg gccgttctgc tcacttaagc ggaatacaat atattctcca
     3421 ttttcagaca gcttcacagc ctgaagatac agctctccaa aatcccagtc cggagcggct
     3481 gcttctgctt ttaccagagg atggttgtat tccagggcag catcattgat gcctgctgtt
     3541 acagctcttc cctcatgagg caggatcaca tagcagaaat catgatggcc gatatcggat
     3601 tggatatccg ggcggatatt ggcccggagc aggcttaagc tcataccgtt ttcttcgatg
     3661 cctacaccgt atttattttc gttgatcagg gcaataccgc cgctttcctc tgcaaaatcg
     3721 caccatttat ggtggcagac ctcaaaacgg gccttctgcc agctggtatt ggtgtgggtt
     3781 tcacgcttga tataaccggc gctggtatcg cagatcagct ctctggacag cacgttgcag
     3841 gaaaactcgg cttttaccag cttgtgcttc tcatcccagt ccacaatatg ctctacttcg
     3901 atagcaggag aagaacggaa gagacggatc acaaccatcc agtggctctt atttccggga
     3961 gttctcagca cacaggcaaa ttctgcggtt tctccgtcaa aggcctggag gcttaaagga
     4021 gattctgctg taaaatcata ttcaacatcc ttgtagttgg gaaggatatc ccaggcatca
     4081 tacataccgg gcgtatcctg atataggtgg agcttattaa agccgccatt tgcccactca
     4141 cggttcatct ttttgtcaaa caggctgctg atggagccgt ccgcttcaaa ctgaacgtca
     4201 tagaagggcg tttttaccgt taagcctgtg ccgcaggacg aagactcaaa ccagttctct
     4261 tttccggcgc atggggatac ggatacggaa gaaaggggtt ctgcatgagt gtaggtaaag
     4321 tatccccgga ttccccggcg tacaaacgca ccattttcag aaggaatgaa gctggcttcc
     4381 cggcgctttt tgtttaatgg gttaaagaat acggaatcgg aatcatttat aatcgcatcc
     4441 agacgttttt ccaggtcttc ataatcccgc acagcatcac aaaatacagg gttaatgtgg
     4501 cttccgggaa gaatatcgtg gaactggttt aaaaggagct ttttatagca gtctctcagc
     4561 tctgcggcgg gatattcttt tccctgcttc atacggagga cagaaagaag ctctgcagct
     4621 cttaatttgg cctccagacg gcggttaaag tcctttaaca cagccttggt ggtaaaggtg
     4681 ccccggtgca tttccaggta gagttcaccg tcccaggttt ctaactggtc attgtccttc
     4741 agattccggt gaaggaattc atcggctctc atatgctctg ttttcggcat aatactcagc
     4801 ttgtcgaagc ggtgcattaa ttcaaccatt tcttccgtta cgccgctgcc gccgtctcca
     4861 tatccgaaca tggataaggt ctgtccgccg gtttccttat cctggtatgc gtcccagttt
     4921 tcctggatct ggctgggcat attccaggtg atgaaatgag taggaggaac gcaggcgcag
     4981 atgcgggtgc cgtcgatgcc cttccagagg aaattgttgt gggggaagcg gttggtatcg
     5041 ttccaggtgg acattttatt ggatacaaaa taatcaacac cggatttttt caggatctgc
     5101 ggcaggatcc agctgtttcc gaatacgtcc ggcagccagg cattgtcaac ggtgatgccg
     5161 aaggcttctc tgtaataaag ctgtccgtag aggcactggc ggatcaggct ttctgcggtg
     5221 gggatgttgc agtccggctc tacatacatg gcgcctacgg gctcaaactg tccggagcgt
     5281 accttttcct tcagctcttc aaagagctca ggataatact gagacagaag ctcataggta
     5341 tagggctgag tatgggcgta tttaaaatca ggatactggt ccatgagacg catttggatc
     5401 aggatggtac gggcattttt atgtaccaca tggatccggc gccagtagta tgcaatatcc
     5461 agatgggaat ggcctaccag agctacgttt ccgctggaac ggaaatcggt gttttcatag
     5521 atatgtgtct caatgtagga aagagcagct tccacgcctt cccaggtatc aaaatcaacc
     5581 agatttaagg cattatttaa ttctctgttt aagaataccc ggtaatcctc atcaaaatga
     5641 ttggattttg caatatccag caggatggtc agatcgtaaa acagggataa gagcggctca
     5701 ttgagctctg ccagataagc gccttcaaag atctggcggc agccccggca ggagagggca
     5761 tcgggatttc gctcgtcatc cggcttggaa cggttgtatc cctcgatttc aatagtaagc
     5821 tcagcgctgt ccttatttaa ccaggggttt accagaatac ggtcacggtt ggggtcaatt
     5881 cctccggcat aatgtccatt gatctttacg atcatttcag ctgccgtatg cagggtaaac
     5941 cagatatcct tttccttcag ggattcggga atctgtgcat ggccccggat aaaggcggtg
     6001 ccgtcagggc tgaaatacat atccccggtt tttaaggggc ggtaatcgga tgtataggta
     6061 tattcatttt cagcggtctg gaacgcatcg cggatttcca gatcggtaat ttcatacttt
     6121 tctttgatta agctgcgttt gatccaggaa tagcgcaggt ctgtccattc ctcaggacgc
     6181 atacttcttc tgataacttc aacaaaagcc atggctcctc cgttctgtga aaggaatcag
     6241 tgatcccaga aaaatggttt tttgcagatg ggttttatgg taacgtcttc ccccagatct
     6301 ctgaggattt cctggcggat ccagtccaga cagcggttta aaatgatgca ttttgaacat
     6361 tctccgcgga aaatcagggt aagacagcga tcctcatagg atacaaattc cacccagcct
     6421 ccgtctcccc tgatacgggg accaatggta ttgcagatat attcttccat aacagcctcc
     6481 tgatgtattc ttaaggctat tatataaatc ccctgaatat agaaacagga caaaatccat
     6541 gaatcgggtg gccttttcta atggattttg tcctgttgtg ctgttttatg acccaagggg
     6601 gatacggatg gaaattacat tgcagtagta ggggatggag ctgaaagaaa tgtatacctt
     6661 ttctgctcca tagtaggcag taagccgttg atatacgttt aagatcccca catggccgcc
     6721 tttggcagtg gagctctttt ttaaaagttc atccagctca gagggttcaa agccgcttcc
     6781 attgtcattg atattgatca caagttcatc ttcttcagcc gtgatgatca cctcgatcgc
     6841 aggcgtggaa atggggattc ccggtgagtc tacgccgaag ccgtgcttga tggaattttc
     6901 cacaaggggc tgcaggatgg ttttgacgat cagatggtgg ctcagtcccg gagccagatg
     6961 atacatgaaa cggatggact ggccgaaacg ctggttcatc agacgtacat aggcattggc
     7021 atggcggatc tcactggaaa gcggaataag atcagctccg tttgacaggc cgattcttaa
     7081 atattccgcc agatactgga tcatattggc ggctgtgtcg gactcaccac gttttacctc
     7141 tgactggatg cagtccagtg tattatacag gaagtgggga ttgatctgct cggttaaaag
     7201 ccgtatttct gccatatatt tttcagattc ttcctgttta atgcgttcca tctgcttttg
     7261 aatggtatcg tgcatctggt taatggcagt gcacagatgg cctatttcat cgccggttcc
     7321 aagctcctgt ttgtgttcat aacggttctc acggatccgc tccacaatgg ccatgagctg
     7381 tgtgaccgga cgggtaatat agcgggtcat catccgggat atgacaagga gaatacccac
     7441 aacaagaaac aggactatga caaggaaatc aatggtaggt ccgaatatgt cataaaaagg
     7501 ctctttggga acacattcca aaaggataag gcctgatttc gccatatggg acgctgccag
     7561 atagaaatca gaggtctggg ccgctgtgtg cgtttcatct gtttctgtca aagtgcggat
     7621 caaagcatct gtctcaggaa gcattaaagg atttgttttt ttatcatagg atgaggggct
     7681 taagggtacg ccatcctctg taaaaaggta atacgtacct tctgcaagtc ccgtgcttgc
     7741 cagagtaagg ctctggagaa gcttggtaca gtccagatag acgaccacat atacgtcgga
     7801 gggctcatct gttatattta caactgcgta ttcggcctca ttgacccgga taggaacgac
     7861 cagaggaaga acaggcaccc tgcttcgcag tggactttgg cggattggaa ggcaggttac
     7921 tccgtgaagc ccgtaaagct cttcttccgt aaaaaattct gcggtatccg gaagaggatt
     7981 tttataaagg gaataggcag tttttccatc tccggagaga accagggcag actgtaccag
     8041 atagttgcag cctgccagat cggacagttc attttggacc agacgttcct gaacaatata
     8101 ggacacagag ggagaggcca gcatacggat atcggaagaa aattcagccg ttccgcagat
     8161 ggcagtcagc cggtccataa tattcttata gttataactt aaagtctcag aggcattctg
     8221 ggccgcctgc ttcgtcctct caaactgccg gtctgaaagg atgaagctgt aataaaaaat
     8281 ggaaatagca gtaatcagcg tagtcagaac cagggtcagg atcccgatta cgatatttat
     8341 tttccccata agggaggcat ctctaaagtg ccggggtaat tgttttttca cagcaaggcc
     8401 tcctgcctgt tttaagatcg gtattttgcc tgaaagtccg agggtgtaat gccatggaca
     8461 tttttaaatg cccgggaaaa ggagcggtaa tccccatatc ctacatcata agccacatcg
     8521 ctgatcgaag cagagggatt cataagaacg cggcgggcat attccatttt ttctgtcagc
     8581 agataatccc ttaaatttac atttgtctta tttttaaaat aaatggcaaa ataagagggg
     8641 ctcagatgaa cctcacgggc aatatcctcg attttgatat ggtttgtgat gtttgagtgg
     8701 atatattcct tggcagtgca gatgatggga tcatcggctt tggatgcatt ttcctgcacc
     8761 tgagcgtatt tggaagatgc gtagccgtac acagcgtcga taaattccga cagctggcag
     8821 aaggagtcgc acagccactg gcggatctgg gacagctgtt tgaaacggta aaggtctctg
     8881 gcagtagcaa tttctgatat ttctccgtgg gaaaaattgg aaaactcctg ctgaacctga
     8941 tggaacaggg cgtagcacag gctgtaaaca tagtttggcg gcggcatagg tacatagaga
     9001 aggctgtcaa taaattcatc gcaataggct ttgatcttat ctctgtcctt ttttacaata
     9061 tactgaaaaa gaggaaggta gtctatatca gacagccggg ttttgggcgg tacgctgcag
     9121 atcgtctcat aagtgaaaat gtgggaggag gaatcataca gctgataggt aagagcagtc
     9181 agcgcccggt tgtaggaata ggaacattcc atcagtccgg ataccgtatc tccgacgccg
     9241 atcaggggca gcgaaagtcc ggcatttttt aaggatttca gacagcgcag ggcaacatga
     9301 aaggggatca ggctggaggt attgaagatt ccgatgagct gtttggcatc atgcttccag
     9361 aaaatgcatt tttcaccggc aaattcctga ttcagctgct ttaaaacagg atcaggggag
     9421 aatgaggctg tgccggaaga aagggtttct tcaaatataa aagcgcagac atagcaggaa
     9481 ctgtcgctta aaggcagctg cgtatcgcac agcatccggg aaataatacc ggattccagg
     9541 attttcccat caatgagatt attgaaaaaa ttaaaattaa actgctcctg atacagacgc
     9601 cgggtgctgt gacggctctg gctggttctt tccataaaaa tacggcagat ttcgtccgta
     9661 agctcggtac tgttcagagg ctttaaaaga taggccttgg ctccgtaccg catggcctcc
     9721 tttgcataag aaaagtcatc atagccgctt aatatgataa aatcggtaga gattcccgca
     9781 ttgcggcagc gctcaatgac ctggatccct gtaaggtctg gcatccgaat gtcgataatg
     9841 gccagatccg gcttatattt aatgatagca tctaacgcct catggccgtt ggacgccagg
     9901 gctgcaatac ggattcccag actttcccag tcgattgcgg tttttactcc ttccagaacg
     9961 gaatattcat cgtctgccag aacagcagta aattcaacgg tattttgttc catgagggtc
    10021 ctcctgtggg cgaaaataaa tagatgatgg tacagacaga taatcagaaa tatggtgtga
    10081 tttcgattat atacgccagt ataatgactg tcaagcatgg gatttgttac atgagacagc
    10141 ataaaaattg ttacatggaa gataaaatat gtccttctga ttgtgaaaaa taaatgataa
    10201 agttatgcca tcgacaggta agaaggaaac agaaaataaa aaattttaag gaggaagtta
    10261 tgagaaagaa acgtttcatc agcattgctc tggcagcagt gatggcagct tctgttgtag
    10321 gctgcggttc cggttccgac acggcagcga atgtagaaaa tacccaggca gagggcggag
    10381 aagccaaggc tcctgagggt gcgactacag tagaggtatg gacagaggac cgtcacgatc
    10441 tggagtatgt agaaaagatg attgatcagt acaatcagaa taatgatgac aatattttca
    10501 tcaatctaac tgttattaca gaggattata agaatatgct ggctctggca tataacggcg
    10561 gaaccgctcc tgatgttgta ggcgcaaaca gccttccatt aaatacattt gcagatacag
    10621 gaattctgat gcctttaaac gagtacattg attccgatga aaccttccag aaggtaaacg
    10681 agccatatga gcacgcatat gagggcgaga acacaagaaa cggaaatatc tactttgtat
    10741 acagcggtat gcgttccggc gttcgtgtag aatataataa gaatattctg gaagagtgcg
    10801 gttatacaga agttccttcc aaattagaag attacattga tatggctaag gatattaccg
    10861 aaaagggagc aggcaactat tatggtatcg gatttacatc cgcatctacc tttgagcgtc
    10921 ttctggaaat gtccgctcag gtttccggta tttactatta tgactatgta aacggaaaat
    10981 atgacttcag cggctataag gatattgttg aacttggaaa gagatttatt tctgaagaca
    11041 tcgcatatcc ggatcagcag ggcgttgaca acatgagagc gctgtttgca gaaggccagt
    11101 ttgccctgtg gagcaacgct tctcaggaag ccagcgtatt taccaatcag attccgatta
    11161 ccgagtttga gtggggcgta gcagaggttc cgtccctgac aggtgagatt gagggcgcac
    11221 tgcagacaac tccttccaag gcatacggta tcgtatctac ctctgagcat aaggatgagg
    11281 catggaaggt aattcagtac ttccagtctg aagagttctt aaaaggatat ctggagagcg
    11341 gccactgcct gccgatcagc acctatatgg atgctgcaat cgacaagacc aagatcggac
    11401 gtatggcaga tttctcactg cttgattatg agtccgtata tccgaagcct ccggtaatta
    11461 acctgacagg agatgactac agagttgtgc tttggaatgc agtaatggat tatgtagacg
    11521 tagatgaggc catcgaagat ctgaataccc gttacaatga ggctctggag gcagacgtgg
    11581 cttccggcgc taccaagaga ctggtgattt ccgattatga tcctcttcat ccaagcgacg
    11641 gaacgatcac atatctggat aaataagcaa taattgacag aggggatccg gctgctggaa
    11701 tgtaccggtt ccccttcggt ttttacagcc tgtatgcagg aggttttaga tgaacacgaa
    11761 gaagaaaaaa tttaagatta caagggacgg tattgcggcg gcgctgatgc tgactccctc
    11821 caccatcctt ttagctgtgt gctccatcta tccatttatc tggattttcc ggtatgtatg
    11881 ttttgactat aatggattta cagctaccta taccggaagc cggaacttta tgagaatgtt
    11941 gtcggataag gcattctggg gaagcgtggg acacactatg gaatacgcag tctataagct
    12001 ggcctttatt atccccctgg cgctgctttt agctgtactt ttaaatgcaa agatgagagg
    12061 aagcaatttc ttcagaggca tttattttat gcctacggtt atcagtacgt ctatttccgg
    12121 tatgatcttt gcatttattt ttgcaactca gaacggtatt gctaatgaaa tgcttttaag
    12181 tgcacacctg attgattctc ccattaaatg gctgaccagc aaggaatggg ttatggtggc
    12241 tgtaacggta ctggcagtct ggggcggtct tggaaactat atgctgtatt ttactacggg
    12301 aatgaacagc gtggcagagg acgtatatga atcggccaag atcgacgggg caggcgcagt
    12361 ccagaccttc ttttatatca cactgcctat gctggctccg gttcttaagg ttgttctgat
    12421 gctggctatt accagcgcat ttaaggatta cgaggcgatc atggttctct ccaagggagg
    12481 tccgggaaac cgctctatgg taatgttctt atacatttac aacctgatct ttggaactac
    12541 caatacaacc actcaggcac agatcggtta cggcgctctc ttaagtgttg tggcagctct
    12601 gatcgtaggt attatcacag caatctatct gtttgtatcc agaaaactgg atgatgtagt
    12661 ataaggagga aaaccatgga aaaaggtcat gcggtgtatt ccaccaaatc gaaaatttta
    12721 cgagggattg tctgggtgct tatgctgatc ctggcgctct ttatggtatt cccgatcctt
    12781 tatatcatac tgggttcctt taaggaaaac tcagagcttt tagtaggcgg aacaaatatc
    12841 cttccaagcc actgggtatt ttccaattac tcagatgcat ggaagcaggc taattttgct
    12901 acctacacta tgaacagtat tttcctggct ctgggtgtta tgacgattac gcttcttaca
    12961 tctactatgt ccggatatgt attttccaga aggaatttcc ggggaaaaga actcctgtac
    13021 agcctgtttg tagcgtttat gtttatcaat gtgggttctg tttcattaag acctctgttt
    13081 gaacttgcgg taaagctgca tatgaataag aatcttttct cggttatcct tatttcggta
    13141 ggaaccggac aggcaactta tattttcctt tgcagaggct ttgtaaactc ccttccaaag
    13201 gagctggatg aggcagccaa gattgacgga tgtaccttct ttcagaccta ccgtctggta
    13261 atcgtgcctc tgttaaagcc ggttatggct actatcgcgc ttttgtcctt tagacagggc
    13321 tggaacgagt acattcttcc gctggtattt actatgacaa atgataaatt aagacctctg
    13381 acggtaggtg taaatatgct gaaaaatgca ggggacggcg ctgcagcctg gaacattatg
    13441 tttgcaggtg cgaccatcgc tattattcct atgcttatta catattgtat tttcagcaga
    13501 tactttatgg gcggtatgac taccggcgca gtaaagggat aaaaatgagc cgcttatctt
    13561 cagataggcg gcttatttta ataaatgagc ctgcgggcgt atgatgaagg agaaaacgga
    13621 tgaaagaatg gatgctgggc catgacggat ttatcatgga atatatgatc gcggggccga
    13681 aggttacgcc ttttaagtcg gatgagaggg cggaaaacca gctggagctg gaagcgcgtc
    13741 tcagagcgca gatcgtaacg cctaaaaagg aggaatacca ggtcgatccc cgattgggac
    13801 aagaggcaga gaacgggtgc agatggtctg tatgggctcc gggaaataac tgctttattg
    13861 acgtatccca tttctacagc acgcttcaga gcgtcagcct gttagctgct gtgaacctga
    13921 atgcagatac agcctgtgag gtgcaggcca ggatctggac ctatatggca gtggggatct
    13981 actgcaacgg gaaactggca ggagaggtaa aacggccggt ttataagccg atccagtatc
    14041 aggatgtgat atttcagctg aatcagggaa agaatctgat cctgtgtgaa tgtgagaatc
    14101 tgggtgtgcg ggataccaga aatatagtgg gaatccagat cgtgagccat agggagcaca
    14161 taaagacagc actgccggac gaccggttcc aggagcaggt atttgaggat acggagttta
    14221 tgaggcagct ttgtctggaa cagggaagtc ttgtgatgcc tgaaattgca ggggcagaga
    14281 catctgtctg ctttcacagg gacagtccgg actatgaggt tatgtgcctt ccccaaaagg
    14341 agatttctct tgagggaaag aagatcctgc ctgtgccgga agaaacagcc cttatatccg
    14401 taatacggaa gcgcccagaa tatgagctga gccgtgagct ggaatttaca gaacgccgtc
    14461 tcccctgtta tccgtcatcg ggtatgacag aagaagaaaa ctggaagcgg atcttaaagg
    14521 aaattgcatc tgtgggaagc ctgaaccggg gaaaattcgg ttttgcggtt tttaatatcc
    14581 tggcaagaaa gcagctggga atccatgacc ctaaggatag gcagcgctta tttgaaacgc
    14641 tggatctgat cgaggaacgg gtggactgct cagattttct ggtctgcggc ctgatccggt
    14701 atatgcatct gtatgagatg gatgaaaagc tgcaggagag aacaaagcag gtgcttttaa
    14761 attaccggta ttggatggat atggacgggg cggacgccat gtgcttctgg agcgaaaatc
    14821 attccctgat gttttactct gctgccatgg aagccggaaa gctttaccct gaggagtatt
    14881 tcccgcgggc cggcatgaag ggagaagcac ttcagaaatg gggacgcagc cgtcttttgc
    14941 agtggctgga tgatacagaa gaatacggtt ttgaggaatt tttaagcacc gtctatatgt
    15001 gcgtgacctt tgcggctctg ttaaatgtga ttgattttgc cgaaaaggag atttcagacc
    15061 gggcaagggc actggaggac cacctggtgg acgaactttg tactcagacc tttaaaggaa
    15121 gcatcattgc gcctatggga cgggtttaca gagaggtaat ttatcccttc tgccagggag
    15181 cacagtctct gatcaaccgg atcgacccgg aggcgccctg ggcttacgga gagggatggc
    15241 tgggctttct ttcaggaagc acctaccgtt tcccacaggg gatgaaggaa cggatgaagc
    15301 aggatatatc ctgccgctgt acctctggaa atgctgccat cgttcttgaa aaaacgcagg
    15361 attactgctt aacctccgta gaatctccca gagcagaccg tgacgccaga tgggaaaata
    15421 tccgctccgg gcagggagaa aaagtccata cccatgcatt taccaaatca ttaaatgaat
    15481 gcttccatgg aacctctgct ttcgggccgg gaatttacgg ttatcagcag catatgtggt
    15541 atggagcact gtcaccggag gcagtgatat ttgcaaacca tccgggagcc acctcggaac
    15601 aatccgatat gcggccggga tactggtatg gaaatggtgt gatgccggct gtaaagcagg
    15661 ttccgggaaa agtgggtgcg gtttaccgga ttcctgaaag ccatcccatt cattttaccc
    15721 atatttactg ccccagggac cggtttgacc gtgtgctgaa agccggaaac tggctcctct
    15781 taaaaaagga tacaggctat atgggattgt ggtgcagtac agagctgacg ccctttgacg
    15841 atacgatctt tcactgtgaa ctcagggctt atggtgatga tatggcatat ctgtgcgtct
    15901 gcggcagtac ggagaaatgg aacacactgg aagaatttgg aaactgggta caaagcctga
    15961 aaccgtctta ctgccgggaa acgggaacct taaaaacaga ggatggcttc gaacttcact
    16021 atcaggcggt gcaggacgat acgcagtatc ttttataaaa ataaaaaagg gaaggaaaac
    16081 agagaagaat cttaccagga tcaaggcgcc agttatcttt aaggcggcag aaaacgtaag
    16141 ctggagcagc ccaaggctct atataaagga tggagtaaag accattgtga tgaatcgtca
    16201 tgtaagcgat ggagatcaca cagaaaccgt ccaggtggca agcagcagcc gggatttaac
    16261 atactggaca gttccgtccg tttttgaggg cggacagccg gagggagaag caggaaagga
    16321 agcagagccc tcccggagct ggacacagtt tccgtgggca gcagccggga tccatgccgt
    16381 aagcgcagtc tgggatacag aacttgaaat gtatctggct gtctatgagg gacaggaaac
    16441 cacagggaaa ttgtccggga gcatcggcct tgcagtcagc aaagatctga tccactggca
    16501 gagctttttc agggatcagc ccacctatcc cggtcatggc gtaatctatg agggagatta
    16561 ctatccatcc attccttttg gagagtatat ttattccatg gagcttccgg caggagaaaa
    16621 tgtgtttgat gtgcgtgatt acggagcggt ccccgatgga gaaacccttt ctacagaagc
    16681 ggttcaggca gcagtggatg ctgcggcagc ctgcggggga acggtactgg ttgcaggcgg
    16741 ctattattgt gtgggaactg tttatgtggc ttccggcgtt accctgtgga tccagaagga
    16801 cagcgccctg tgtgcttcca gagatttaaa gcagtataaa gacgccctga ttgcctgcgt
    16861 aaatgcagaa aatgtaaaaa tcacaggagg cggaaaaatc attggaaggg gagaatattt
    16921 tgtctatctg ccaaagaatc cgcctttgac ggttcctatg gagacaacta agcttccgcc
    16981 ctttttatat gatcccatgg gatatccggt agattccatc cgctatacat accgcagccg
    17041 gatccgttat gcggaggacc gctatgcgga aggacttcct tcgattccaa gacccatgta
    17101 cactctgtgg gtgcggggct gtaccaatgc ggtgatcgaa aatatcataa tcgaggatgc
    17161 tctggactgg accctcagca ttgactgcag ccatcaggtt atggtacatg atctggtaat
    17221 caatggaaac cgccatgtgg caaatacaga cggaatcgac atcatgggaa gcagccaggt
    17281 cgaaatcgac cactgctttg tatcctgtgc cgacgacggg atctgtgtga aatcccctat
    17341 gaagcagggg catgacggga ttaccattga ggatgcagat gcagaaatga agggaaccga
    17401 gcaggttcat attaaaaact gtaccgtcgt ttccgtaatg aatgcattta agatcggaac
    17461 agagacatac tttgatatta aggatattac cgttgaaaac tgccgcttta tgctgccgga
    17521 catttatccg ggaacggttt ccggaatttc cattgaatct gcagacggaa gccggatcca
    17581 gaatgtccag atccgcaata tagagatgga ccgggtctgc tgtcctgttt ttatctgcct
    17641 gaacatgaga aacaagtatg gatttttaaa tgaaacggat cggcaggaaa aagccttcgg
    17701 aggagccatt gaacatgttg taattgagca tgtacgggct tataatacag aggttccgag
    17761 tattatcaca ggctttcaga cggatgagaa cggagttcat acggaacgcc gtgtatcaga
    17821 tatcaggatc cgtgatttta aggctgttta ccatgataat aaggaggttc tggatattcg
    17881 gactcctgtt tatgagaatg tccgcgatta cccggaaaat aacgcatttg gagatgtacc
    17941 tgcatatgga ttttatatcc gccatgcaga cagagtcact ctggaggact gggaggttat
    18001 ccccagaacc atgaatacca gggaagcgct tgtacgggaa tatacagatt gagaggaaaa
    18061 aaggatatga gcaccgttta tgagacgatt aaaaatgagt atgaggtatg gaaaaagaag
    18121 gtcacagagg atgcagacct gacagcagag cttacggaca tggagggaaa ggaagaccgg
    18181 attacggatg cattttatca gaatctggcc tttggaacag gaggactgcg cggcgtgatc
    18241 ggcgcaggaa ccaaccgcat gaatatttat accgtaggaa aggcctctca gggactggca
    18301 gattatatca accgccattt cccaaaagag gagcggaaaa ttgcaatcag ctatgacagc
    18361 cggattaaga gcagcctgtt tgcaaagcgg gctgcggagg tttttgcggc caatgggatt
    18421 agggtattta tctatacgga gctgatgccc actccgatgc tgtcctgggc agtacgcagc
    18481 ttaagctgcg ccgcaggcgt aatggtaact gcaagccaca atccttccaa atacaacgga
    18541 tataaagtat atggcccgga cggctgccag atcacaacag atgctgcagc agaaatcctt
    18601 ggatgcatcg aaaaaacaga tgcgtttgag gatgtaaaaa gggaagattt tgaaactgcc
    18661 cttgaaaacg gcaggatttc ctatatcggg gaagacacgg tcacttcctt tatcgaggcg
    18721 gtgaagggcc agtccatgct gacaaaatca gaagaaaacg tggataaaaa tatttctatc
    18781 gtatactctc ctttaaatgg aaccggatta aagccggtgt taagaacact taaagaaagc
    18841 ggctttacca atattacggt tgtaaaggaa caggagcagc cggacggcca ttttccgacc
    18901 tgtccctatc ctaatccgga gatacgggag gcaatggctc ttggaatgga gtatgcaaag
    18961 aagtgcagtg cagaccttct tctggcgaca gatccggact gtgaccgtgt ggggatcgcg
    19021 gttcccaacc gtgagggcga atatgtgctt ttatccggaa atgagacagg gcttcttctg
    19081 ttaaactata tctgcagcat gagacaggca gcagggacta tgccggagca cccggttttt
    19141 gtgaaaacca ttgttacaat ggatatggca gccagaatcg cagctgatta cggagtaaag
    19201 acagtggatg tgctgaccgg attcaaattt atcggggatc agatcgcacg cctggaagca
    19261 gagggaagag agaatgatta tatcctgggc tttgaggaaa gctacggata tttaagtgga
    19321 agctatgtcc gtgataaaga tgcggtagac ggcgcgtttt taatctgtga gatggctgca
    19381 tattaccgtt ctaagggaat ggggctttta gaagtgcttg aggggctgta tgaaaaatat
    19441 ggtttctgcc ttaacaccct tcattcctat gaatttgagg gaatggaagg ctttgaaaag
    19501 atgaagggaa tcatgaaccg ccttcacacc atggcttccc gtatagcgga gggagaggca
    19561 gtctgcatcg gcggacgaaa gacagaagcc gtagaggatt atctgtccgg tacgatcagc
    19621 agggcggaca aaacgacaga gccgatcgca ggccttcctt cctcggatgt gctgaaattc
    19681 cgtctggccg gaaatgcgtc tgtggttgta aggccttcag gaacagagcc caagttaaag
    19741 ctgtacattt ccgtgagtgc agaagacaaa gcgaaagctc aggcagagga agagaaaatc
    19801 acagctcaga tggaagagtg gtttttataa gaaaaagaac aggactgatt gaaaattcct
    19861 tcttgacttt agcaagttaa cttgctatac tgtccctaaa atgtacatcg aaagatggag
    19921 tatcccatat acgtccgctt aaaaccgggc gcatacccca acaaaaacca aaggaggatc
    19981 agtaccatgt catatgttga tgagatttat gagagagtgg tagcacagaa tcccggtgag
    20041 ccggaattcc accaggcagt aaaggaagtt ttggattcct taaagctggt gatcgacgca
    20101 aatgaggaaa agtaccgcaa agcgggcatc ctggagcgtt ttacagagcc ggagcgcatt
    20161 atttcgttcc gcgtgccgtg ggtggacgat cagggaaatg tgcaggtcaa taaaggttac
    20221 agagtgcagt ttaacagcgc cattggacct tataagggcg gacttcgttt ccatccatcc
    20281 gtaaatcaga gtattcttaa gtttttgggc tttgagcaga ccttaaagaa ctctctgacc
    20341 ggtcttccta tgggcggcgg aaaaggcggc tccaattttg atccaaaggg caaatccgac
    20401 cgtgagatca tggccttctg ccagagcttt atgacagaac tgtaccgtca tatcggcaag
    20461 gatacggacg ttcccgc
//
LOCUS       DBMDUH010000031        20313 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_1628_length_20313_cov_7.251039, whole genome shotgun sequence.
ACCESSION   DBMDUH010000031 DBMDUH010000000
VERSION     DBMDUH010000031.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 20313)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 20313)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20313
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1628_length_20313_cov_7.251039"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="ACZPCG_04315"
     CDS             <1..540
                     /locus_tag="ACZPCG_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP-loop family protein"
                     /protein_id="HIR1284334.1"
                     /translation="DIVVDVDQSPCYLCARMRRGYLYSHAKELGCNKIALGHHFDDVI
                     ETILMGILYGGQINTMMPKLHSTNFSGMELIRPLYFVKEEDILAWRDSNHLHFLQCAC
                     QFTEQIAKERAAAGETVNGGDIVHTSKRQEMKELIARLRKNSPFIDANIMKSVENINL
                     DACLGYVQKGVRHHFLETY"
     gene            611..2215
                     /locus_tag="ACZPCG_04320"
     CDS             611..2215
                     /locus_tag="ACZPCG_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 and LysM peptidoglycan-binding
                     domain-containing protein"
                     /protein_id="HIR1284335.1"
                     /translation="MELIKKQIHMNQWKGNVATQITLDDDFIVPDTMDDMEQVMLDNG
                     EIQAEQVKPMGDKVNVKGRLEFQVLYRREGGGLQTLGGSIPFEEMINVPQLEDRDTVS
                     LSWTLDDLNTNMIHSRKLGVQAIVTLQVRIEASKDTEAAVDVKQGGTDTEAYTGAAEN
                     HGEMPVETLKRRMTAASMAVCRKDTYRIREAVGLTGGKPGIGRLLWKEMKLRAVSVKP
                     LDGTLHLEGELTVFVIYEAEDDTMPVQWLEETIPFSGEMEAEGVKEDQIPMITVRLSH
                     REIEAKPDYDGEMRELEIDGVLELDIRLYEELETELLSDVYSNSRELTPERRETVFDR
                     ILTRNVCRSRIGEKVKLPQGERILQICHHSGTIKLDEVEPGEDCLKIEGVLEVTLLYL
                     TSDDQAPVQPAVEQIPFKCTAEARGIREDSVYQLDAGLEQLTAVMMGGDMVEIKAVAV
                     LDFLVLQPVKEQVITGISSGPLDLKKLQELPGIAGYIVQPGDSLWMIAKKFHTTISHI
                     ISANELADEEIKAGQRLLLVKEVARG"
     gene            2422..3708
                     /gene="larA"
                     /locus_tag="ACZPCG_04325"
     CDS             2422..3708
                     /gene="larA"
                     /locus_tag="ACZPCG_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HIR1284336.1"
                     /translation="MKTIKIPYYTSSLDLHVDEKNLEAVITARTDEYEAGKSEEELVK
                     EALEHPIGTPRLSELAKGKNRIVLVTSDHTRAVPSKLTLPLLLAEIRKGNPDADITIL
                     IATGLHRPTTEAEQRRMFGDPIVDHEKIAVNKAFEPDDFAYVTGLPSGAELWVNKLAL
                     DCDLLITEGFIEPHFFAGFSGGRKSILPGICNAATVNENHSYKAISSPYAATGVLEHN
                     PIHEDMVYAARAVNVQFILNVALNGEKKVIAAFAGDLEKAHEKGVEFVRGLAQCPGMT
                     GDIVITSNGGYPLDQNLYQSPKAVATAEACCKDGGVIIMCASCFDGMGGTHFEKLIVQ
                     GTVDEIDAYLSKIPPRETIPEQWCAQIYSRILKKHKVILVTTYLDHEMVRKANMIPAS
                     SPDEALEMAYEMMGRDARVIVIPDGVAVLAVKEQED"
     gene            3710..4021
                     /locus_tag="ACZPCG_04330"
     CDS             3710..4021
                     /locus_tag="ACZPCG_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HIR1284337.1"
                     /translation="MELKLALKVDDLDNVATIFADDITDGTVVEVRDKKGQKEEICVH
                     GDVPYGHKIAVTDIPKGAHIMKYGESIGAASTDIKKGDYVHVHNLDALRGRGDLEEVE
                     Q"
     gene            4018..5199
                     /locus_tag="ACZPCG_04335"
     CDS             4018..5199
                     /locus_tag="ACZPCG_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HIR1284338.1"
                     /translation="MSEYTFQGYRRANGKVGARNLVAVIPSVICANDVGQAICRQVQG
                     TIGYFHHQGCCQLPIDLKRVTDTLSNLGQSPNVGAALIVSLGCEGTDHERMVEEIRAA
                     GKPVEIIRIQELGGTSRAIQAGIDAAQRLVQEISGLQRETVDISEVVMAIKCGASDTT
                     SGMASNCVIGYVADKLVDLGATVVFGETTEFLGGEHILAKRAVGGENGPIGQKIYEIV
                     DRMEKRAKSVGEDMRGGQPTPGNIAGGLSSIEEKSLGAIVKSGHRPIQGVLEYCDRVD
                     GQKGLWIKDAPGREPEILTGMAATGAQFMMFSTGRGAPQGFPSMPVIKICGNPHTYER
                     MQHDMDLNAGRIIQGEKTIEEVGEEAFRLMLDVLSGKMTKNEALGYYGSMDIFCLGPV
                     I"
     gene            5236..6690
                     /locus_tag="ACZPCG_04340"
     CDS             5236..6690
                     /locus_tag="ACZPCG_04340"
                     /inference="COORDINATES: protein motif:HMM:NF014500.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HIR1284339.1"
                     /translation="MLNPVLAMAIGIVVMMVLIIFTKMHAFPSLIISAILIAVLAMPF
                     GPEGATVATLTECVSTVTKGFGNTMASIGVVIGFGCIMGIFLEKSGAAKRMALTILKM
                     VGVKNCDVVLGLTGFVVSIPVFCDSGFVILSSLAKEFSRLTKKSMVWLGGILGMGLYI
                     THFMVPPTPGPLAVVGTFQENGYNLDLGMYIIFGILISVPLFIFSVFLFRYFGKKYPD
                     LVVPYEIDRSKYSEGQLKVLDRIQEKQSRNEDLATEDFADLLAAEKLPSAFLSFATLL
                     VPIVLIFANTLVGQIGALQGTAFAECVKFLGQPVMALFISLCMGAFLLCAEYDRKTCV
                     DLMQEACRDAGPIVFITAAGGALGAVINATGAAKMMADAIVAAGVPGILVPIIIGVIM
                     RFPQGSGTTAMITGSAIIAPMISTLNINPYLAALSLCLTTMCPSYLNDSYFHVVTNFS
                     GMDIKTSLKTWTIGSILVPAFGSAIFCILSIFIH"
     gene            complement(6800..7465)
                     /locus_tag="ACZPCG_04345"
     CDS             complement(6800..7465)
                     /locus_tag="ACZPCG_04345"
                     /inference="COORDINATES: protein motif:HMM:NF019349.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1284340.1"
                     /translation="MSQIRRITTIKQQVYEILRDDICSGVYAPGQQIQETELSKKLEI
                     SRSPIREALRQLVSEGLAVEYPNRGVFVKTYTPKDIEDVFDLRILLESYAIMRSGNCL
                     TLEKHHELNDCMERLKTYHSQDDIQRYVEEDTLLHQLLIQLSGNNLVISTYERVYAMV
                     QRFRIYSLKSRKRFDDSLTEHIQIIQNVLAGNVKEADRINREHLLLAKDAIIVYLDQM
                     HQD"
     gene            7619..8347
                     /locus_tag="ACZPCG_04350"
     CDS             7619..8347
                     /locus_tag="ACZPCG_04350"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HIR1284341.1"
                     /translation="MDEKWQQLKEWIQESQYIVFFGGAGVSTESKIPDFRSEDGLYRQ
                     KYKYPPETIISHSFYMKNTEEFYRFYKDRMIYKEAVPNDAHRALAKLEQEGKVKVVIT
                     QNIDGLHQMAGSREVLELHGSVHRNYCTRCGQFYDLDYITNSEGVPRCTCGGTVKPDV
                     VLYEEGLDDTVLRRSIEHISKADLLIVGGTSLAVYPAAGLIDYYRGKKLVLINRSATP
                     RDSQADLIINDSIGQVLGAVAGIR"
     gene            8362..9357
                     /locus_tag="ACZPCG_04355"
     CDS             8362..9357
                     /locus_tag="ACZPCG_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006782768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR1284342.1"
                     /translation="MYQAAENRYDHMKYRRGGRSGVLLPEISLGLWQNFGLEKPLEEQ
                     REILLRAFDLGITHFDLANNYGAPARGKAEENFGAVLASDLASYRDQLFISTKAGYDC
                     WPGPYGNWGSRKYLMSSLDASLKRMGLDYVDVFYHHRPDPDTPLEETMGALSDMVRQG
                     KALYVGISNYEAEEAERAIAILKENKTPCLLHQPRYNMFERWCEDGLFEVLDREGVGC
                     ICYSPLAQGALTGRYLKGIPEGSRASRSNTTVGGRYLTPERLEKIRALNEMAENRGQS
                     LAQMALSWILRRKEVTSVLIGASSVAQLEDNIKALDGPVFTKEEQEAIDRILADS"
     gene            9410..9625
                     /locus_tag="ACZPCG_04360"
     CDS             9410..9625
                     /locus_tag="ACZPCG_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HIR1284343.1"
                     /translation="MTEKPDYQVGDIVKLKKPHPCGSSEWEILRVGADFRLKCTGCGH
                     QIMVARRMVEKNTRGLKKSGGEMSTNP"
     gene            9749..10036
                     /gene="rpsF"
                     /locus_tag="ACZPCG_04365"
     CDS             9749..10036
                     /gene="rpsF"
                     /locus_tag="ACZPCG_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003501071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HIR1284344.1"
                     /translation="MNKYELTVVLNVKLEDEERAAALEKVKGYITRFGGTVTNVDEWG
                     KKRLAYEIQKMHEAYYYFIQFESDSVCPNEVEAHVRIMEPVIRYLCVKAEA"
     gene            10084..10560
                     /locus_tag="ACZPCG_04370"
     CDS             10084..10560
                     /locus_tag="ACZPCG_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIR1284345.1"
                     /translation="MNRVILMGRLTRDPEVRYSQGERSMAIARYTLAVDRRGRRNQDG
                     NSEQTADFINCVAFDRAGEFAERYFRQGMRVLVSGRIQTGSYVNKDGQKVYTTEVILD
                     DQEFADSKGAASDMGGYGQSGYGQSAPSQRPAPASAIGDGFMNIPDGVEDEGLPFN"
     gene            10603..10872
                     /gene="rpsR"
                     /locus_tag="ACZPCG_04375"
     CDS             10603..10872
                     /gene="rpsR"
                     /locus_tag="ACZPCG_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HIR1284346.1"
                     /translation="MAFNKTERPEGAKMRRPGGMRRRKKVCVFCGDKNGVIDYKDVNK
                     LKRYVSERGKILPRRITGNCAKHQRALTVAIKRARHIALMPYTCE"
     gene            11441..12940
                     /locus_tag="ACZPCG_04380"
     CDS             11441..12940
                     /locus_tag="ACZPCG_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005335347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HIR1284347.1"
                     /translation="MALLNYDVLKKSGYDGYILEDAPVKVLQFGEGNFLRAFVDYWFD
                     LANEKAGWNGKCCLVQPIAPGLAKMINEQEGLYTLYLRGSENGQKVDDKRVISSVANC
                     LNPYEKADYDKMMEVAASDDLEIIVSNTTEAGIQYDPSCQKADCPPASFPAKLTQVLY
                     HRYQAGKSGIIMLACELIDNNGKELLKCVNQYIEQWGLEDGFKKYVNEDCTFCGSLVD
                     RIVPGRIRDAEEVKALDAKNGYEDPLTDVGEVFGVWVIEGDEKLNDVLPFKKAGLMDK
                     VFVTPDMSPYKKRKVRILNGAHTGFVLGAYLAGEDIVRDCMNDEVIKGFMNKMLYDEV
                     IPTLPLDKDDLLKFAAAVSDRFNNPFVNHELMSISLNSTSKWKARNMPSFLEYIKEEG
                     RLPECLTMSLAAYIAFYSNDIQALTDAGLVCKRPAGNEYTVSDDRWVLEFYYDHKDDS
                     EEELVHAVLTNEKMWDQDLTQIEGLEAKVTENLKKIHTEGAKAAYASCL"
     gene            13059..13541
                     /locus_tag="ACZPCG_04385"
     CDS             13059..13541
                     /locus_tag="ACZPCG_04385"
                     /inference="COORDINATES: protein motif:HMM:NF022641.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HIR1284348.1"
                     /translation="MRNRKRRMENFTFYDYREICTHLEQMVQNGWMLKRIGLFWEYER
                     IEPARLHFAVSHCRGIKEEIEEKDGGRLPQDGWKQVCQSDRMGIYCCSREEAPPFGST
                     AKEELEAVRGIGIKRYLFSGLLVFAAALLQEWTFFHLWAGILQECCLTGAASWEAFVF
                     "
     gene            13541..14446
                     /locus_tag="ACZPCG_04390"
     CDS             13541..14446
                     /locus_tag="ACZPCG_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284349.1"
                     /translation="MVVFICLGETIGYILWYLRAKKEVLKGRLPSARGMGIVRRILFG
                     MLSFMMILWTLRTLFGGGLMERWLVVLGVACFSLLFGIVYAGMGWMKKRGLSGRGHQI
                     AGMIAAYFISCFMLGFLTLGILKASDRGMFISDEKETYEYGGRTWEVSQDELPLKLED
                     LTGESYEEYIRENDKKESLLLRQESISQRPRLDAENFRELPELEYSLITVKLPVLYDR
                     CRQALIREGECHYPEGDRIYQEEDARPWGAIEAYRIYDSRYGFRNQYLICWENVLAEI
                     HFPWEPDEGQKGIVGKNLNKLALNS"
     gene            14611..15072
                     /locus_tag="ACZPCG_04395"
     CDS             14611..15072
                     /locus_tag="ACZPCG_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR1284350.1"
                     /translation="MLMPSIFGEDMFDEFMRDFPFFDERDERRAEKKLYGRRGKNLMK
                     TDVKETDQGYELEMDLPGFTKDEVKVSLENGYLTIQAAKGLDQDEQEKDTGRYIRRER
                     YAGACERSFYVGENMVEEDIKGEFKHGILKLFIPKKEEKPAIEQKKYIMIE"
     gene            15190..15708
                     /locus_tag="ACZPCG_04400"
     CDS             15190..15708
                     /locus_tag="ACZPCG_04400"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR1284351.1"
                     /translation="MANPVITITMENGDVMKAELYPEIAPNTVNNFISLVSKGYYDGL
                     IFHRVISGFMIQGGCPQGTGMGGPGYCIKGEFSQNGFKNDLKHTEGVLSMARAMHPDS
                     AGSQFFIMHKTSPHLDGAYAAFGKITEGMEVVNRIADERTDYSDRPLSEQKIKSMTVD
                     TFGETYPEPEKC"
     gene            15674..16495
                     /locus_tag="ACZPCG_04405"
     CDS             15674..16495
                     /locus_tag="ACZPCG_04405"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1284352.1"
                     /translation="MERHIRNRRSAEMTKERRIRIDGTDHRLLISDEPQALLAAKAAG
                     RAVLGVENAVQGDWSLRGIPYVVPGWEYDTEELEELILRRHLGLPWMIDLTERLMIRE
                     FVKGDVKQIPKEEYAGEEAVFRSEETMEQYIRSQYAFYEYGTWALVRRETNELVGMAG
                     VSSPGAEIFEQYALRNLPEKSQWLELGYHIFEPYRKLGYAREAVTAIAEYAHEVLSAE
                     LLAVIHEKNQASRAVAECLGMRVVEERKLSHRDVTAQGNGTDRKWPQGYLLYAEC"
     gene            complement(16428..17111)
                     /locus_tag="ACZPCG_04410"
     CDS             complement(16428..17111)
                     /locus_tag="ACZPCG_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284353.1"
                     /translation="MTLRQEAQEEKEKLKHMSWKDRIWYIWEYYKFHMLLAFIAMVIL
                     WVIGTSLYRQSFTTRLSMAVINDRSGGNSSTEPLMAGLREALDCGKKDIIEINEGLFL
                     DTNEETMSQYSYASMAKIAALVSGGMLDIMIADEATIRHYETLDAYLDLSELLPKELC
                     SQLEDQFLYTTTTDGEKIPAAVSLEKSSLKDDTGIIMEPPYLAVISNTQHKEDILGAI
                     SYLFRSLEP"
     gene            17285..18019
                     /locus_tag="ACZPCG_04415"
     CDS             17285..18019
                     /locus_tag="ACZPCG_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HIR1284354.1"
                     /translation="MLQGLFNYDNPVWRFIGKLGDLILLNILWIVCSIPIVTAGAATT
                     AVYYVTLKLVRDENDGTIKNFFHSFKENFVQSTIIWVILLAAGALLAFDFWFFLTGQM
                     NSLTGVPKLLMTAVFGGFLMLYIFISTYVFALQSRFYNPVKRTLFNAFFMSVRHLLQT
                     IGIIALDVLTAAAAYFGVFFLPQVAMLLILFGFPLIAFINSYFFTAIFQKYMPKKEEH
                     KETDMVPLMDDDNKEVEEAINNLKGN"
     gene            18163..18642
                     /locus_tag="ACZPCG_04420"
     CDS             18163..18642
                     /locus_tag="ACZPCG_04420"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HIR1284355.1"
                     /translation="MYQSHEFYLRRAIEISKEARAAGNTPFGALLVNKDGEIIMEQGN
                     IEITEKICTGHAEATLAARASHEYSREFLWDCTLYTTAEPCAMCAGAIYWANIGRVVY
                     GMTERRLLELTGSNEQNPTFDLPCREVFARGQKDIKVIGPVEAVEVEAAKVHEGYWN"
     gene            18662..19960
                     /locus_tag="ACZPCG_04425"
     CDS             18662..19960
                     /locus_tag="ACZPCG_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015851 - nucleobase transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease family protein"
                     /protein_id="HIR1284356.1"
                     /translation="MKKESFDEKVGWGRMLLVGFQHVLTMCPGTIAVPLILAGALGLD
                     EKETAFLVSANFFTSGIAILIQVLGIGKLAGSRYPIVLGSSFAPLSPMILIGKEYGMA
                     ALFGSVIASGAVIFLLSFFMDKILKLFPQVVVGTFVTLIGITLAPTALRDLAGGEGNA
                     GFGSVENLILGLAVLIFILMVEKYGKGIWKSMSLLLGITAGTAAGCFLHMVDIASIAQ
                     AEIFQPVQPFAYGVPEFKLWPIFLMTIFCIINMIQCIGVFSVMDEIADVETENEIKER
                     GIRGQAVAQMITGAFNSVPSTMFNENVSLIGLTKIKSRSVITTAGIMIILAGIFPKIS
                     AVFTAVPKCVLGGATLALFGVITSSGISMLSRLDFSKDNNFKIVGTSIAIGVGAAFAS
                     DAFVNIPGTLQMILSNGLFMVSISAILLNLLLNGKKAFRK"
     gene            20057..>20313
                     /locus_tag="ACZPCG_04430"
     CDS             20057..>20313
                     /locus_tag="ACZPCG_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006774393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1284357.1"
                     /translation="MATQKLRIKPLSGQAKEAILQYIEEMDLKRDNKLPREEALAEML
                     GVSRITIRQALNDLASEGIIFRRQGRGTFVNVDSLDIKVTF"
BASE COUNT         5515 a         4043 c         5611 g         5144 t
ORIGIN      
        1 gatattgtgg tggatgtgga tcagtccccc tgttacctct gcgccagaat gcggaggggt
       61 tacctctatt cccatgcaaa ggagctgggc tgcaataaga ttgcactggg acaccatttt
      121 gatgatgtga tcgaaactat tcttatgggg atcctttacg gcggccagat caataccatg
      181 atgccaaagc ttcacagcac caacttttcc ggtatggagc tcatacggcc tttgtacttt
      241 gtaaaggaag aggatatttt ggcctggagg gacagcaacc accttcattt cctccaatgt
      301 gcctgccagt ttacggagca gatcgcaaaa gaacgggcgg ctgccgggga aacggtgaac
      361 ggtggtgata ttgtccatac ttccaagcgc caggagatga aggagctgat cgcaaggctt
      421 aggaaaaaca gcccctttat tgatgcgaat atcatgaaaa gtgtggaaaa tatcaatctg
      481 gacgcttgtt tgggctatgt ccagaagggt gtgcggcacc attttttgga aacctattga
      541 gagttatatt taacctgcca tttgcataga ttctttttat aagagactat gagggatggc
      601 aggtgttttt atggagctta taaagaaaca gatacatatg aaccagtgga aaggaaatgt
      661 ggctactcag atcaccctgg atgatgattt tattgtgcct gacaccatgg acgatatgga
      721 gcaggttatg ctggataatg gagagatcca ggcggagcag gtaaaaccta tgggggataa
      781 ggtaaatgta aagggacgtc tggaatttca ggttctgtac cgcagggagg gaggcggact
      841 gcagacgctg ggaggcagca ttccttttga ggagatgatc aatgttcccc agttagagga
      901 tagggataca gtgagtctgt catggacgct ggatgacctg aatacgaata tgatccattc
      961 ccgaaaattg ggagttcagg cgatcgttac ccttcaggtg cggattgagg catcaaaaga
     1021 tacagaagct gccgtggatg taaagcaggg ggggacagat acagaggcct atacaggggc
     1081 tgcggagaat catggggaaa tgccggttga aaccctaaag cgccggatga cagccgcatc
     1141 tatggcagta tgcagaaagg atacctatcg gattcgggag gcagtagggc ttacaggcgg
     1201 aaagccgggg atcggccgcc ttttatggaa agaaatgaag ctgcgggctg tatcggtaaa
     1261 gcctcttgac ggcactctgc atctggaagg ggagctcacg gtatttgtga tctatgaggc
     1321 ggaggatgat acaatgcctg tgcagtggct ggaggaaacg atcccgtttt caggagaaat
     1381 ggaggcagag ggagtaaagg aggatcagat ccccatgatt acggtccggc tttcccatcg
     1441 agagatcgag gcaaaaccgg attatgacgg agaaatgcgg gaactggaga ttgacggggt
     1501 tcttgagctg gatatccgtc tgtatgagga actggaaaca gagcttttaa gcgatgtata
     1561 ttccaacagc cgggaattga cgccggagcg tagggaaacg gtgtttgacc ggatccttac
     1621 acgaaatgta tgccgttcca gaatcggaga aaaagtgaag cttccccagg gagagcggat
     1681 tttacagatc tgtcatcaca gcggaaccat aaaactggat gaggtagaac cgggagagga
     1741 ctgcttaaag atagaggggg ttttggaggt gacacttctt tatcttacca gtgatgacca
     1801 ggctccggtt cagccggcag tagagcagat tccctttaaa tgtacggcag aggccagagg
     1861 gatccgggaa gacagcgtat accagctgga tgcggggctg gaacagctca cagccgtgat
     1921 gatgggagga gatatggtgg aaattaaggc tgtggcagtg ctggacttcc tggttctgca
     1981 gcctgtaaag gaacaggtga tcacaggtat ctcttccggc cctctggatt taaagaagct
     2041 tcaggagctg ccgggaattg cgggatacat tgttcagccc ggtgacagtc tctggatgat
     2101 cgctaaaaaa ttccatacga ccatcagcca catcatatca gccaatgaac tggcggacga
     2161 ggagattaag gccgggcaga ggcttcttct ggtaaaagag gtggcaaggg gataaaaaag
     2221 gaaacagact gttcatggaa aagtaattct atggagggtc tgttttttat gacagtcata
     2281 tgtgtaaaaa tatcaaaaaa agttgaaaac aaaataaaat attggaaaat attgacatta
     2341 agctgcaaat gtaatagaat gaaaaagtag acagtcgaca atttaaaaag acggattaaa
     2401 attcagggaa ggtgatgaag tttgaagact attaagattc catattacac ttccagtcta
     2461 gatctccatg tggatgaaaa aaatctggag gcggtgatca cggcccgcac agatgaatac
     2521 gaggcaggaa agagtgagga ggagctggta aaggaggcgc tggagcatcc catcggaaca
     2581 cccaggctca gtgaactggc aaagggaaag aaccggatcg tgcttgttac ctcagaccac
     2641 accagggccg taccaagtaa actgactctg cctttgcttc tggcagagat ccgcaaagga
     2701 aatccggacg cagatattac gattctgatc gctaccggcc ttcaccgtcc taccacagag
     2761 gcagagcagc gcaggatgtt tggcgatccg atcgtagacc atgagaagat tgcggtaaat
     2821 aaggcatttg agccggatga ttttgcctat gtgaccggtc ttccctccgg agcggagctg
     2881 tgggtaaaca aactggcctt agactgcgat ctgctgatta cggaaggatt tattgagccg
     2941 catttctttg ccggcttttc aggaggacga aagagtatcc tgccgggtat ctgcaatgcg
     3001 gcaaccgtaa atgaaaacca ttcctacaag gccatttcca gtccctatgc tgctacaggc
     3061 gttttggagc acaatcccat ccatgaggat atggtgtatg cggccagagc agtgaatgtt
     3121 cagtttatct taaatgtagc attaaatggg gagaaaaagg taattgcagc ctttgcagga
     3181 gatctggaaa aggctcatga aaaaggcgtg gagtttgtcc gcggtctggc tcagtgtccg
     3241 ggcatgacgg gagatatcgt tatcacctca aacggcggtt atcctctgga tcagaacctg
     3301 tatcagagtc ccaaggcagt ggccacagca gaggcctgct gtaaagacgg cggagttatc
     3361 atcatgtgtg cctcctgctt tgacggtatg ggcggcactc attttgaaaa gttgatcgtt
     3421 cagggaacgg tggatgaaat tgatgcttat ttgtcaaaga ttcctcccag agagacgatc
     3481 ccggagcagt ggtgtgcaca gatctattcc aggatcttaa agaagcataa ggtgattctt
     3541 gtgaccacct atctggatca tgagatggta cgcaaagcca atatgatccc ggcctcttcg
     3601 ccggatgagg ctcttgaaat ggcctatgag atgatgggac gggacgcaag ggtgattgtt
     3661 attcccgatg gcgtggcagt gctggcagta aaggaacagg aggactaaga tggagcttaa
     3721 gctggcgtta aaggtagacg atctggacaa tgtagctacc atttttgccg atgatatcac
     3781 agacggcacg gtggtagagg tccgggataa aaaggggcag aaggaagaaa tctgcgtcca
     3841 tggggatgtt ccctacggtc acaagatcgc agtgacggac attccgaagg gagctcatat
     3901 tatgaaatat ggagaatcca ttggagcggc gagcacggat atcaaaaagg gtgattatgt
     3961 ccatgtgcat aacctggatg cattaagagg cagaggcgat ctggaggagg tggagcaatg
     4021 agcgaatata catttcaggg atatcggaga gccaatggaa aggttggcgc ccgcaatctg
     4081 gtagctgtga tccccagcgt tatctgtgct aatgacgtag gccaggcaat ctgccgtcag
     4141 gtgcagggaa ccatcggata ttttcatcat cagggctgct gccagcttcc cattgactta
     4201 aagagagtga cggacaccct ttccaatctg ggacagagcc ccaatgtggg agcggcgctg
     4261 atcgtcagcc tgggctgtga gggaacagac cacgagcgga tggtggaaga aatacgggca
     4321 gccggaaaac cggtagagat tatccggatc caggagctgg gaggaacctc cagggcaatc
     4381 caggccggaa ttgatgcggc tcagagactg gttcaggaga tttccggact gcagagggaa
     4441 acagttgata tttctgaggt agtcatggca attaaatgcg gtgcttccga tactacctcc
     4501 ggtatggctt ccaactgcgt gatcggttat gtggcagata agctggtaga cctgggagca
     4561 actgtggtat ttggggaaac cacagaattc ttaggcggcg agcacattct tgctaaacgg
     4621 gcagtaggcg gagaaaacgg ccccataggc cagaaaatct atgaaatcgt agatcggatg
     4681 gagaaacggg ctaaatccgt aggagaggat atgagaggcg gtcagcctac accgggcaat
     4741 attgcaggag gactttccag tattgaggaa aagagcctgg gagcgatcgt aaaatccggc
     4801 catcggccga tccagggagt tttagaatat tgtgaccggg tagacggaca gaaggggctg
     4861 tggatcaagg atgctccggg acgggaaccg gagattctga ccggaatggc ggccacggga
     4921 gcgcagttta tgatgttctc cactggaaga ggagcacctc agggctttcc gtccatgcct
     4981 gtaatcaaga tctgcggcaa tcctcataca tatgagcgga tgcagcacga catggatctg
     5041 aatgcaggac ggatcattca gggagaaaag acgatcgagg aggtaggaga agaggccttc
     5101 cgtctgatgc tggatgttct ttcaggaaaa atgacaaaga acgaggcact gggctattac
     5161 ggatccatgg atattttctg cctgggtcct gtaatctgat atttggggtt taatcatgta
     5221 agggggtaat tatttatgct gaatcctgta ttagctatgg caatcggtat tgtcgttatg
     5281 atggtactga tcatttttac aaaaatgcat gcatttccgt cactgatcat ctcggcaatc
     5341 ctgattgcgg ttcttgcaat gccatttggt ccggagggag ccacagttgc gaccctgacc
     5401 gaatgtgtaa gcaccgttac caagggcttt ggaaacacta tggcaagtat cggcgttgta
     5461 atcggctttg gctgtatcat gggaattttc ctggagaaga gcggcgctgc caagcgtatg
     5521 gctctgacaa tccttaaaat ggtcggtgta aagaactgcg atgtggttct tggacttaca
     5581 ggcttcgtag tttccattcc cgtattctgc gattccggtt tcgttatcct gtcctctctg
     5641 gcaaaagagt tttcacgcct tactaagaaa tccatggttt ggctgggcgg cattcttggt
     5701 atgggacttt acattaccca ctttatggtt ccgcctacac cgggtcctct ggcagttgta
     5761 ggaacctttc aggaaaatgg ttataatctg gatctgggaa tgtatatcat ttttggtatc
     5821 ctgatttctg ttcccctgtt tatcttttct gtatttcttt tccgttattt tggaaagaaa
     5881 tacccggatc tggttgtgcc ttatgagatc gaccgttcca agtacagtga gggccagtta
     5941 aaggttcttg accgcattca ggaaaaacag agccgcaatg aagatcttgc tacagaggat
     6001 tttgcagatc tgctggctgc tgagaagctt cccagtgcat ttttatcctt tgccacctta
     6061 ttagtaccca ttgttctgat ctttgccaac actctggtag gacagatcgg agcgcttcag
     6121 ggaactgcat ttgcagagtg tgtgaaattc ctcggacagc cggttatggc gctgtttatt
     6181 tccctctgca tgggcgcatt ccttctgtgt gcggaatatg accgcaagac ctgtgtggat
     6241 ctgatgcagg aagcctgtcg tgatgcaggt cctatcgtat tcatcacagc agcaggcggt
     6301 gcccttggcg cagttatcaa tgcgacaggc gcagcaaaga tgatggcaga cgcaatcgtt
     6361 gctgcgggag ttccaggtat tttagtaccg attatcattg gtgtcatcat gcgtttcccg
     6421 cagggctccg gaaccacagc tatgattaca ggctccgcga ttattgcccc gatgatcagc
     6481 acactgaata tcaatccgta tcttgcagca ctgtcactct gtctgacaac catgtgccca
     6541 agctacttaa acgattccta tttccacgtt gtaaccaatt tctccggtat ggatatcaag
     6601 accagcttaa agacatggac gattggatct attctggttc cggcatttgg ttcagctatt
     6661 ttctgcatcc tgtctatctt tatccattga tgggaagaat gcaggactgt gatatgatat
     6721 gaaaaaagag ctgtctcttc taatttggga gatggctctt tttttgcggg tgcgccggct
     6781 gtctttgtat ggaaatacat caatcctgat gcatctggtc cagatatacg ataatggcat
     6841 cctttgcaag aagcaggtgt tcccgattga tccggtctgc ctctttgaca tttccggcca
     6901 gtacattttg gataatctgt atatgctcgg tcagggaatc gtcaaaacgc ttccggcttt
     6961 ttaaggaata gatgcggaac cgctgaacca tggcgtagac gcgttcatag gtactgatta
     7021 ccagattatt tccgctgagc tggatcaaaa gctggtgaag cagagtgtct tcttccacat
     7081 aacgctgtat atcgtcctgg ctgtgatagg tttttaagcg ttccatgcag tcgtttaatt
     7141 catggtgttt ttctaaggtc agacagtttc cggaacgcat aatggcatag ctttccagca
     7201 ggatccgcaa atcgaacaca tcttcaatat cctttggagt gtaggttttt acaaatacgc
     7261 ctcggttggg atattctacg gcaagtcctt cggataccag ctggcgcagg gcttcacgga
     7321 tggggctgcg ggaaatctcc agtttttttg acagctctgt ttcctggatc tgctgtcctg
     7381 gcgcatagac gccgctgcag atatcatctc ttaatatctc atatacctgc tgtttgattg
     7441 tagtaattct tctgatttgg ctcatgtatg ttatctccag gatcgaatgt ttaatttctt
     7501 ttatcatatc atcggaggga gcataagtaa acggatggga gcaattttgt ttttcatagt
     7561 gaatctaaac gaaacccgtg ctataatagg ttcaaaatcc ggcaaaggag gaaaactcat
     7621 ggatgaaaaa tggcagcagt taaaggagtg gattcaagaa agccaatata tagttttctt
     7681 tggaggtgct ggtgtttcta ctgagagcaa aattccggac tttcggagcg aggacgggct
     7741 gtatcgtcag aagtataagt accctccgga aacaatcatc agccacagct tttatatgaa
     7801 gaatacggag gagttctacc gtttttataa agacaggatg atttataaag aggctgtacc
     7861 caatgacgcc cacagagcgc ttgcaaagct ggagcaggag gggaaggtaa aggttgttat
     7921 tacccagaat attgacgggc ttcatcagat ggcaggaagc agggaggtgc tggagctgca
     7981 tggctccgta caccgaaatt actgcacccg ctgcggacag ttttatgatc tggattatat
     8041 aacgaacagt gaaggtgttc ccagatgcac ctgcggcggc acagtcaaac cggatgtggt
     8101 tctgtatgaa gagggactgg atgatacggt tctgcgccgc tctatcgagc acatttcaaa
     8161 agcagatctg ctgatcgtgg gcggtacgtc tctggcagtc tatccggctg ccggcctgat
     8221 tgactattat agaggaaaga aacttgtgct tatcaaccgt tctgccactc ccagagattc
     8281 ccaggcagat ttgatcatta acgacagtat cggccaggtc ttaggagccg tggccggtat
     8341 cagatagagg aggaaataat tatgtatcag gcagcagaaa acagatatga ccatatgaaa
     8401 tacagacgcg gcggaagaag cggcgtactg cttccggaga tttctctggg gctgtggcag
     8461 aattttggac tggaaaagcc tctggaggag cagagagaga tccttcttcg ggcctttgat
     8521 cttgggatta ctcattttga ccttgcaaat aattacggcg ctccggccag aggaaaggcg
     8581 gaggaaaatt ttggagccgt tctggcatcc gatctggctt cctacagaga ccagctgttt
     8641 atttccacca aggccggtta tgactgctgg cccggtcctt acggtaactg gggctcccgc
     8701 aaatacctga tgtccagtct ggacgctagc ttgaagcgca tgggccttga ctatgtggac
     8761 gtattttacc atcaccgtcc ggatccggat actcctttag aggaaaccat gggcgctctt
     8821 tccgatatgg tgcgccaggg aaaggccctt tatgtgggaa tttccaatta tgaggcagag
     8881 gaagcagagc gggccattgc cattttaaag gaaaataaga ccccgtgtct gcttcatcag
     8941 ccccgctaca atatgttcga gcgctggtgc gaagatgggc tttttgaggt tctggaccgg
     9001 gaaggagtcg gatgcatttg ctacagccca ttggctcagg gggctttgac gggacggtat
     9061 ttaaagggga ttcctgaggg ctccagagcg tcacgcagca acaccacagt aggagggcgc
     9121 tacctcacgc cagaacgcct ggaaaaaatc cgcgcgttaa atgagatggc agaaaatcgt
     9181 ggtcagagtc tggctcagat ggctctttcc tggatccttc gcagaaagga agtgaccagc
     9241 gttctcatcg gcgccagcag cgtcgcccag ctggaggaca atataaaggc tctggacgga
     9301 ccagtattta caaaagagga acaggaggcc attgaccgta ttctggcaga ttcctaagaa
     9361 caggcagaat agggacaagg gtagaggaat aaaaaaggga gagcggatta tgactgaaaa
     9421 accagattac caggtgggag acattgtgaa gcttaaaaag cctcatccct gcggcagcag
     9481 cgaatgggag atcctgcggg tgggggcgga tttccgctta aaatgcactg gctgcggcca
     9541 tcagatcatg gtggccagga ggatggtgga gaagaatacc agaggcctga aaaagagcgg
     9601 cggagaaatg tccacaaacc catgaaaaaa gcgaaaaaag ggcttgcatt taagcaaaac
     9661 ctttggtaaa ataactattc gtgacatatt aaatccttgc tcctgaaact gaaagcaggg
     9721 gccagagacc acaaggaggt aaagcaagat gaacaagtac gaattaaccg ttgttctgaa
     9781 tgtgaagctt gaagacgaag agcgcgcagc agcactcgag aaggtaaagg gttacatcac
     9841 ccgtttcggc ggtactgtaa ctaacgtaga tgagtggggt aagaagagac ttgcatacga
     9901 gatccagaag atgcatgagg cttactacta cttcatccag ttcgagtctg attctgtatg
     9961 cccgaacgaa gtagaagctc acgttcgtat tatggaacca gtgatcagat acttatgcgt
    10021 taaggctgag gcataagaga tacccagacc gcttaagcgg aattgaaaag gaagtgtgac
    10081 cgtatgaaca gagttatctt aatgggcaga ttgacaagag atccggaagt aagatattcc
    10141 cagggagagc gttctatggc gattgccaga tacactctcg cagtggacag aagaggccgc
    10201 agaaatcagg atggaaactc cgagcagact gcagatttta tcaactgtgt ggcatttgac
    10261 cgcgcaggcg agtttgcaga gcggtatttc cgccagggca tgcgtgttct tgtatccgga
    10321 cgtatccaga caggaagcta tgtaaataag gacggtcaaa aggtatacac caccgaggtc
    10381 atcttagatg accaggagtt cgcggatagt aagggtgcgg cttctgatat gggcggatat
    10441 ggacagtccg gttacggaca gtctgcgcct tctcagagac cggctcctgc cagcgcgatt
    10501 ggtgatgggt ttatgaatat tccggacgga gttgaggacg aaggacttcc attcaactag
    10561 accgggattc ttcacggtga ataataaagg aggacaacac caatggcatt taataagact
    10621 gaaagaccag agggcgctaa gatgagaaga ccgggcggta tgcgcagaag aaagaaagtc
    10681 tgcgtattct gcggcgacaa gaatggcgta attgactata aggatgtaaa caagctgaag
    10741 agatacgtat ccgagagagg aaaaatcctt ccccgtcgta tcactggcaa ctgcgcaaag
    10801 caccagagag ctttaactgt agctatcaag cgcgcacgcc acatcgctct gatgccttat
    10861 acatgtgaat aattttaaca tgggaagtcc ctggaaacct agagtttccg gggatttttt
    10921 tcgctttttt gtactcaaat gtttataagg gcgtacacct tttactcaaa aattgttcaa
    10981 gtttaatatc cctcttggcc tggggtatga atggggtata ataggcctgt acaggcataa
    11041 gcctgaaatg aagaaccgga cataccggaa catatttcct ttgtattcga tgcacagtat
    11101 tattcagata ataatccgga tttgagagaa atatacggtg atgatctgcc gtcctattat
    11161 aatcattata tggactgtgg ctgggctgag gaacggattt gccattaaaa aatttataaa
    11221 gagcggaaaa gttgtctgtt ttcttatgaa agagccatgt aagaaaatgt aacattgata
    11281 aaaaatatac aataatgcga aacacaacaa attttacaaa aatacagcta taattttcat
    11341 gttttctaca aaataaaata tttctattta aaaagggtaa atacctgtgg taaaataaag
    11401 tacaaagatg gttcacaaag gaaaggagag aacatttatt atggcacttt taaattatga
    11461 tgtgttgaag aaatccggct atgacggata tatcctggag gacgcccctg taaaggtcct
    11521 tcagttcggc gagggaaact tccttcgtgc atttgtggat tattggtttg atcttgcaaa
    11581 tgaaaaggcc ggatggaacg gaaagtgctg tctggtacag cccattgctc cgggtctggc
    11641 aaagatgatc aacgagcagg agggtctgta caccctgtat ctgcgcggaa gtgaaaatgg
    11701 tcagaaggta gacgacaagc gtgtgatttc cagcgttgca aactgcttaa atccatatga
    11761 gaaggctgac tatgacaaga tgatggaggt tgccgcaagc gacgatcttg agattatcgt
    11821 atccaatacg acggaggcag gtattcagta tgatccttcc tgccagaagg ctgactgccc
    11881 tcctgcaagc tttcctgcaa agctgacaca ggtgctctat catcgctatc aggctggaaa
    11941 gtccggtatt atcatgctgg cctgcgagct gatcgacaac aacggcaaag agctgttgaa
    12001 gtgcgtaaat cagtacattg agcagtgggg acttgaggac ggatttaaga agtatgtcaa
    12061 tgaggactgc accttctgcg gttccctggt agaccgtatc gttccgggcc gtatccgtga
    12121 tgcagaggaa gtaaaggctc tggatgcaaa gaacggctac gaggatccgc tgacagacgt
    12181 tggcgaggta ttcggcgtct gggtaatcga gggtgacgag aagttaaatg atgttctgcc
    12241 atttaagaag gccggactga tggacaaggt atttgtaaca cctgatatga gcccatataa
    12301 gaagagaaag gtgcgtatct taaacggcgc tcatactggc tttgttctgg gcgcatacct
    12361 ggcaggcgag gatattgtcc gcgactgcat gaacgatgag gtgatcaagg gcttcatgaa
    12421 caagatgctc tatgatgagg tgatcccaac ccttcctctg gacaaggatg acctgttaaa
    12481 attcgcagca gctgtatctg accgtttcaa taatccattt gtaaaccatg agctgatgag
    12541 catttcctta aattctacct ccaagtggaa ggcaagaaat atgccgtcct tcctggagta
    12601 catcaaggaa gagggcaggc tgcctgagtg cctgaccatg tctctggctg catacattgc
    12661 attttacagc aatgatatcc aggcccttac agatgcaggt cttgtatgca agcgtccggc
    12721 cggcaatgag tataccgtaa gcgatgaccg ctgggtactg gaattctact acgaccataa
    12781 ggatgattct gaggaggagc tggtacatgc tgttctgacc aatgaaaaaa tgtgggatca
    12841 ggatttgaca cagatcgaag gtctggaggc caaggttaca gaaaacttaa agaagattca
    12901 tacagaaggc gcaaaggctg catacgcaag ctgcctgtaa tcgccggaaa atgtgttaaa
    12961 accgctgaaa gcagtgccgc agaaagcctg aaatgggctt ctgcggcatt tttcagttaa
    13021 atggagaaga atgtggcaaa aattaagggg gtgtgcctgt gagaaaccga aaacgccgaa
    13081 tggagaactt tacattttac gattacagag aaatctgcac ccatctggag cagatggttc
    13141 aaaatgggtg gatgctcaaa aggatcgggc tgttctggga gtatgaacgg atcgaaccgg
    13201 caagactgca ttttgcagtg tctcattgca gggggataaa agaggagatt gaggaaaagg
    13261 acgggggccg gctgccacag gatggctgga agcaggtctg ccagtcggac cgtatgggaa
    13321 tctactgctg cagccgggag gaggcgcctc cttttggtag tacggcaaag gaggagctgg
    13381 aagcggttcg tgggattggg ataaaaagat atcttttttc agggctcctt gtttttgcag
    13441 cagccctgct gcaggagtgg actttttttc atctctgggc cgggatttta caggagtgct
    13501 gtctgacggg ggccgcctct tgggaggcat ttgttttctg atggtggttt ttatttgcct
    13561 gggcgagaca atcggttata ttttatggta tttacgggca aagaaggagg ttctaaaggg
    13621 caggctccct tctgcgcggg gaatggggat agttcggagg attctgtttg gaatgctgtc
    13681 gtttatgatg atactctgga cgctgagaac gctgtttggc ggaggtttga tggagcgctg
    13741 gctggtggtt cttggagttg cctgtttcag ccttttattt ggcatagtgt atgcgggaat
    13801 gggatggatg aaaaaaaggg gcctgtcagg gagaggacat cagatcgcag gaatgatcgc
    13861 cgcatatttt atttcgtgtt ttatgctggg atttcttacc ttgggaattt tgaaggcgtc
    13921 tgatagaggc atgtttattt cagatgaaaa agaaacctat gagtatgggg gaagaacctg
    13981 ggaggtgtct caggatgagc ttcctcttaa gctggaagat ctgacggggg agagctatga
    14041 ggaatatatc agagaaaatg ataaaaagga atccctgctt ctcaggcagg agagcatttc
    14101 ccagaggccc cgcctggatg cagaaaattt cagggaactg ccggaactgg agtatagcct
    14161 gataactgtg aagctgccgg tgctttatga ccggtgcagg caggctctga tccgggaagg
    14221 agagtgccat tatccggagg gagacaggat ttatcaggaa gaggatgcga ggccctgggg
    14281 agccatagaa gcataccgta tctatgattc cagatatggt ttccgaaatc agtatctgat
    14341 atgctgggaa aatgttcttg cggaaatcca tttcccatgg gagccggatg aggggcagaa
    14401 ggggatcgtg ggaaaaaatt taaataaatt agcactcaac tcttgacatt gctaataata
    14461 agtgttatat tacagctaga acaaaggaaa agagttcgga agcaggaact tgattccgga
    14521 ggttcccata gtggatcggc agacggattg gaagatataa gatcgcagat cacagatcca
    14581 aaaaggtttc acgaaaagga gagatgattt atgttgatgc ctagtatttt tggagaagat
    14641 atgtttgacg agtttatgag agattttccg ttttttgatg aaagagatga aagaagagct
    14701 gaaaaaaagc tgtacggacg cagaggcaaa aatctgatga agaccgatgt gaaagagaca
    14761 gaccaggggt atgagctgga aatggatctg cccggattta caaaagatga ggttaaggtt
    14821 tctctggaaa atggctattt gaccattcag gcagccaaag ggctggatca ggatgagcag
    14881 gaaaaggata ctggacgcta tatccgcagg gagcggtatg caggagcctg tgagcgttcc
    14941 ttctatgtag gagagaacat ggttgaggaa gatataaagg gtgagtttaa gcacggtatt
    15001 ttaaagctgt ttatcccgaa aaaggaagag aagcctgcta tagagcagaa gaaatatatt
    15061 atgattgaat aatggttttc cagtgggaag gccgtccgga gaagacgccg ggcggccttc
    15121 cttttgctgg acaaattccc tgtgatttgg tatagtggaa caagattaaa tgaaaaggag
    15181 attatgagaa tggcaaatcc agttattact attaccatgg aaaatggcga tgtgatgaag
    15241 gcagagttat atcctgagat tgctcccaac acagttaata attttatcag ccttgtatcg
    15301 aaaggatact atgacggact gatctttcac cgcgtgatca gcggatttat gatccagggc
    15361 ggctgccctc agggaacagg catgggcggc cccggctact gcattaaggg tgagttttcc
    15421 cagaacggct ttaaaaatga cttaaagcat acagagggcg ttctgtccat ggcaagggca
    15481 atgcatcctg attctgcagg ctctcagttc tttatcatgc ataaaacatc tcctcatctg
    15541 gacggcgcat atgcggcatt tggaaagatc acagagggca tggaggtcgt aaaccggatt
    15601 gcagatgagc gcaccgatta cagcgaccgc ccgttaagcg agcagaagat caagtccatg
    15661 acagttgata catttggaga gacatatccg gaaccggaga agtgctgaga tgacaaagga
    15721 gcgcaggatc cgtattgatg gaacggatca caggcttctg atatcagatg aaccacaggc
    15781 ccttctggcg gcgaaagctg ccggaagggc tgttttgggt gtagagaatg ctgtgcaggg
    15841 cgattggagt ctgagaggga tcccctatgt ggttcctggc tgggagtatg atacagaaga
    15901 gttggaggag ctgatccttc ggcgtcatct gggtcttccc tggatgatcg atctcactga
    15961 acggctgatg atacgggagt ttgtaaaagg ggatgttaag cagatcccta aggaggaata
    16021 cgccggggaa gaggcggttt tccggtcgga agagactatg gagcagtata tccgcagtca
    16081 gtatgctttt tatgaatacg gtacctgggc gcttgtgcgc agggaaacaa atgagcttgt
    16141 ggggatggca ggagtatcat cgccgggggc ggaaatattt gagcagtatg ctttgaggaa
    16201 tcttcccgaa aaaagccagt ggctggagct gggatatcat atttttgagc cttaccggaa
    16261 attgggatat gcaagggagg cagtgacagc cattgcagag tacgcccatg aggtactgtc
    16321 agcagaactt ctggcggtga tccatgaaaa aaaccaggcc tcccgcgcag tggcggaatg
    16381 cctgggtatg agagtggttg aagaaagaaa attaagccat agggatgtta cggctcaagg
    16441 gaacggaaca gataggaaat ggccccaagg atatcttctt tatgctgagt gttagagatc
    16501 acagccagat agggcggctc catgataata cccgtatcat cctttaaaga ggatttttcc
    16561 agtgaaacgg cagcggggat tttttcaccg tctgtggttg tcgtatagag aaactgatcc
    16621 tccagctggc tgcaaagctc cttcggcaaa agctcactaa gatccaggta agcatccaga
    16681 gtttcgtaat ggcggatggt agcctcatca gcgatcataa tatccagcat tcctccggat
    16741 acaagagcgg cgatttttgc catgctggca tatgaatact gggacatggt ttcctcattt
    16801 gtatccagaa acaggccttc attgatctcg ataatatcct ttttcccaca gtccagggct
    16861 tccctcagtc ctgccatcaa aggctcggta ctggaatttc cgccggaacg gtcattgata
    16921 accgccatgg aaagccgggt ggtaaatgac tgccggtaca aagaggtgcc gatcacccag
    16981 aggattacca tagcaatgaa tgctaacagc atatggaatt tgtagtattc ccagatatac
    17041 cagatcctgt ccttccagga catatgtttg agtttttctt tttcttcctg agcttcttgc
    17101 cgtaaagtca ttcgtgtcgc ttcctccgtt tgcagatttt tactgtcagc aggctgttgc
    17161 tgatgatacc atttattata ggctatttca catcctcctg caactatttt gagtttgcgc
    17221 atttggggct gatatggtac aatattctgt gatattttta ttttaagaaa gaagagggta
    17281 tattatgctt caaggcctgt ttaattatga caatcccgtc tggcgcttta tcggaaagct
    17341 gggagatttg attcttttga acattctgtg gattgtgtgt tccattccaa ttgtcactgc
    17401 gggagctgct acaacggctg tgtactatgt gactttgaaa ctggtccgag atgaaaatga
    17461 cggtacgatc aaaaactttt tccattcctt taaggagaat tttgtgcagt ctacgattat
    17521 atgggtgatt ctgctggctg caggcgcgct tttggcattt gacttctggt tctttcttac
    17581 aggccagatg aacagtctta caggtgttcc caagcttttg atgacggctg tattcggagg
    17641 ctttctgatg ctgtatatct ttatcagcac ctatgtgttt gcccttcagt cccggtttta
    17701 taatccggtg aagcggacgc tgtttaatgc cttcttcatg tccgtgcgcc atctgcttca
    17761 gaccattggt atcattgctt tggatgtgct tacagctgcg gctgcttatt tcggtgtgtt
    17821 tttcctgcct caggtagcca tgctgttaat tctgtttgga tttcccctga ttgcctttat
    17881 caattcctat ttctttacgg caatcttcca gaaatatatg ccaaagaaag aggagcataa
    17941 ggaaacagac atggttcctc ttatggatga cgataataaa gaagtagaag aagccattaa
    18001 caatttaaaa ggaaattgat tcacatgttg tgaaagctgt cggaaaatga tccgacagct
    18061 ttttttttgc tgtgaactct ttacaaaaaa atgattgtgt gctatagtaa aaacacaaaa
    18121 ggtaatacct attacttaat actttgaaaa ggagagggaa cgatgtatca gagccatgaa
    18181 ttttatttaa ggagagcgat cgagatttca aaggaggcaa gagcggccgg taatactccc
    18241 tttggagcgc tgctggtaaa caaggatggg gaaatcatca tggagcaggg gaatattgag
    18301 attacagaaa aaatctgcac cggccatgca gaggcaaccc ttgcagccag ggcttcccat
    18361 gagtattcca gagagttttt atgggactgt accttatata ccactgcgga gccctgtgcg
    18421 atgtgtgcgg gagcgattta ctgggctaat atcggaaggg ttgtttacgg catgacagaa
    18481 agaaggctgt tggagcttac gggaagcaat gaacagaatc ccacctttga tcttccgtgc
    18541 cgcgaggtgt tcgccagagg acagaaggat ataaaggtca tcggaccggt agaggcagta
    18601 gaggtagaag cggcaaaggt acatgaggga tactggaatt agtattcagt aaggagtggg
    18661 tatgaaaaag gagagttttg acgaaaaagt gggatggggc aggatgctgt tagtggggtt
    18721 tcagcatgtg cttaccatgt gccctggaac cattgcagtt ccgctgatac tggcaggagc
    18781 tttgggattg gatgaaaagg aaacagcctt tcttgtctca gccaatttct ttacaagcgg
    18841 cattgcgatc ctgatccagg tgctggggat cggaaagctg gcaggatcca gatacccgat
    18901 tgttctgggg tcgtcctttg cacccttaag tcccatgatc ctgatcggta aggaatatgg
    18961 gatggcggct ttgtttggtt cagtaattgc ctccggtgcg gtgatttttc ttttatcctt
    19021 tttcatggat aagattttaa agcttttccc gcaggtagtt gtgggaacct ttgtaacact
    19081 gattggaatt actttggcgc ctaccgcgct gagggatctg gcggggggag agggaaacgc
    19141 aggctttggc tccgtcgaaa atctcatcct gggtctggca gtgcttatat ttatactgat
    19201 ggtggaaaag tatggaaaag gcatctggaa aagtatgtca ttgcttcttg gtattaccgc
    19261 aggaacggct gcaggatgtt ttttacatat ggtggatatt gcttccattg cgcaggcaga
    19321 gatattccag ccggttcagc cttttgctta cggagtgccg gaatttaagc tgtggcctat
    19381 tttccttatg acgattttct gcattatcaa tatgatccag tgcatcggcg tattttccgt
    19441 gatggatgaa attgcagatg tggaaacaga aaatgagatt aaggagcggg ggatccgcgg
    19501 gcaggcagta gctcagatga tcacaggagc atttaattcc gtcccatcta ccatgtttaa
    19561 tgaaaatgtg agtttgatcg gtttaacaaa gataaagagc cgttcggtca taacgaccgc
    19621 agggatcatg atcatactgg caggaatttt cccaaagatt tctgcggtat ttacagcggt
    19681 tcccaagtgt gtgctgggag gagctaccct ggccttgttc ggagtgatca cttcatcggg
    19741 aatttccatg ctttccagac tggatttctc aaaggataat aattttaaaa tcgtgggaac
    19801 cagtattgcc atcggcgtag gtgcagcatt cgcatccgat gcgtttgtaa atattcccgg
    19861 aacacttcag atgatcctca gcaacggctt atttatggta agcatcagcg cgattctttt
    19921 aaatctgctt ctcaatggaa aaaaagcctt tcgtaaatga tatactgtat ttcttgacaa
    19981 aaaaaagtga ttcttctatc atcttagtta gaatgtatgt ttaagcacat cggaaacagg
    20041 aaagagagga atcaccttgg cgactcagaa attacgcata aaaccattgt cagggcaggc
    20101 gaaggaggcg atccttcaat atatagaaga aatggatctg aaaagggaca ataagcttcc
    20161 aagagaggag gctctggcag aaatgctggg cgtaagccgg atcaccatcc gccaggcctt
    20221 aaatgacctg gcctcagagg gaatcatttt caggcgtcag gggcggggaa cctttgtaaa
    20281 tgtggattct ctggatatca aggttacctt cag
//
LOCUS       DBMDUH010000032        19731 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_1679_length_19731_cov_6.079980, whole genome shotgun sequence.
ACCESSION   DBMDUH010000032 DBMDUH010000000
VERSION     DBMDUH010000032.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 19731)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 19731)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19731
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1679_length_19731_cov_6.079980"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="ACZPCG_04435"
     CDS             complement(<1..196)
                     /locus_tag="ACZPCG_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284358.1"
                     /translation="MMNVKELKGIDVHLKEIDRLIQLSPKRTVDRSQIRNPKEIERHE
                     QMKQYALRLGINLYMTDDTES"
     gene            complement(539..1429)
                     /locus_tag="ACZPCG_04440"
     CDS             complement(539..1429)
                     /locus_tag="ACZPCG_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HIR1284359.1"
                     /translation="MQYRSVNEIAKKWNVSERSVRNYCAHGRVDGAFLTGKTWNIPEN
                     AEKPERSNKKKEQPITLLDILKEQKVSKYSGGIYHKTQIDLTYNSNHIEGSSLTHDQT
                     RYIFETNTIGVEKEVLNVDDVIETANHFRCIDMIIDNAKGALTEKFIKELHNTLKTGT
                     SDSRKDWFAVGDYKKMPNEVGGMETALPEEVADKMKALLTEYNGKEEKTFEDILDFHV
                     KFERIHPFQDGNGRVGRLIMFKECLKYNIVPFIIEDNLKMFYYRGLKEWNNEKGYLTD
                     TCLTAQDKYKAYLDYFRIVY"
     gene            complement(1464..>1917)
                     /gene="fic"
                     /locus_tag="ACZPCG_04445"
     CDS             complement(1464..>1917)
                     /gene="fic"
                     /locus_tag="ACZPCG_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015561349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HIR1284360.1"
                     /translation="EIYDFAGKLRKVNISKGNFRFAPLMYLEAALQSIDKMPQSTFDE
                     IVEKYVEMNVAHPFREGNGRSTRIWLDLMLKKEIGYVVDWSKVDKVDYLLAMERSPIK
                     DIEIKFLLKNALTNNVNDREIYMKGIDHSYYYEGYYVFKMENLTDIKD"
     assembly_gap    1918..1927
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1928..2082)
                     /locus_tag="ACZPCG_04450"
                     /pseudo
     CDS             complement(1928..2082)
                     /locus_tag="ACZPCG_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005947501.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
     gene            complement(2526..3395)
                     /locus_tag="ACZPCG_04455"
     CDS             complement(2526..3395)
                     /locus_tag="ACZPCG_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HIR1284361.1"
                     /translation="MKIRNMKQVKQVTAHMKDVKMKRLFRRNQIIITTLAVMIAAAGY
                     LNYAGKRDLAAAGNIYEAGMMDISDEDLLAENKAAGDDLLEIGSLDQDSDQVEEALPS
                     EGLAQKEEVGAEAAEQLAEAGENMEAETEQLALAEKGGQDAVSEAGDAQDAPAGIENP
                     GEAVLTSGMSVADYIAGVQLSREQVRARNKETLMSLINSTSIDEAAKQQAIQDMIRLT
                     EISEKENAAETLLMAKGFSDPVVSIADEKVDVVINAPSITDPQRAQIEDIVKRKTEVG
                     ADQIIITLLNMEE"
     gene            complement(3412..4029)
                     /locus_tag="ACZPCG_04460"
     CDS             complement(3412..4029)
                     /locus_tag="ACZPCG_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HIR1284362.1"
                     /translation="MGWKEKLLKLNGKMTREKWLLFLLAGVFLMLLSVPGGEKKTVKE
                     DRPKSGEQAAGTEEHSLVSWDISSSFSSRSYEERLEKRIRELLRSVDGVGQVDVMVVL
                     KSTGQKVLRVDRSGSSTVTKETDSAGGSREITESQSEESTVLPQSGGGSNGPVVEKEL
                     CPEISGIVVSADGGGSPEVCSEISQAMEALLGLPAHKIKVLKRVE"
     gene            complement(3983..4705)
                     /locus_tag="ACZPCG_04465"
     CDS             complement(3983..4705)
                     /locus_tag="ACZPCG_04465"
                     /inference="COORDINATES: protein motif:HMM:NF021121.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HIR1284363.1"
                     /translation="MAEALLGWARNIIYYMVFLSLAEHLLAGSSYGRYLRLFGGLVLV
                     LVVLGPFGSLGDWEKQVSSLFDQFTFRQESRELKKEIFGMEEKRLAGITGQYRENVEK
                     EITALVKAQGIVCSQVSVQMEENKESSSYGKILAIEIEVSGEDFFEQEGFAQEGAGQE
                     GSGRENIRAAGQMLLSPISEERPVSIGRIEIGAGEKREDKEGVKQELSEREKQAAETL
                     KGKVETYYGLEGEAVKIKWKND"
     gene            complement(4752..6002)
                     /locus_tag="ACZPCG_04470"
     CDS             complement(4752..6002)
                     /locus_tag="ACZPCG_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007709357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HIR1284364.1"
                     /translation="MAQGRKAVKYGRIGLKLIVFLMALWISGRGSTITAGAFYEKENF
                     ENNKETSEEMEIGQAAHDMGISEELEQLQKYLDRSLGGKNGTVDLLFTDLMKAFASGN
                     LGALGTAIKEGFGQLLFSQVREGAGLLTQAAAIGLLGAMFSGAASVFKNGQISDTGFY
                     VAYLLLFACLAGSFLTSLSIAAEVLDRILEFMGVLIPAYFMAVSFSGGSITGICMYEA
                     MMAAVFCVQWLSKSVFLTGVRVYVLLVLGSHVMKEPLLSKMTSLTEQAVEWGIKTMFG
                     LILGLHVLEAMVLPYADSVGRSGLLKLAEAVPGLGAGAGAAARMVLGSGVLIKNTMGA
                     AGVTVLALIAFLPAAKLLTLALFYQAAGAVMQPVCDKRMVSCVIGVSRGHRLLLKIVL
                     YSLLLFVIAIAVTCKAADVAYLSA"
     gene            complement(6050..6436)
                     /locus_tag="ACZPCG_04475"
     CDS             complement(6050..6436)
                     /locus_tag="ACZPCG_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAC/SpoIIIAD family protein"
                     /protein_id="HIR1284365.1"
                     /translation="MSILTIGAAGITAVLLAVWLKGVRAEYALYMVLGAQILFMICGT
                     GRLEVILDAMRRLEGYIKIDPAYLGALLKMVGITYVAEFASGICKDAGYSALGNQIEI
                     FGKLSILGVSMPVFLALFEILDQFMG"
     gene            complement(6445..6639)
                     /gene="spoIIIAC"
                     /locus_tag="ACZPCG_04480"
     CDS             complement(6445..6639)
                     /gene="spoIIIAC"
                     /locus_tag="ACZPCG_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006779097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HIR1284366.1"
                     /translation="MDVNLIFRIAAVGILVSVICQVLKHSGRDEQAFLTSLAGLVLVL
                     FWMVPYIYDLFETMKRLFLL"
     gene            complement(6666..7196)
                     /locus_tag="ACZPCG_04485"
     CDS             complement(6666..7196)
                     /locus_tag="ACZPCG_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HIR1284367.1"
                     /translation="MVIKWAGIGLVLFSGTGWGMWMAWRWRARLEALETLRQMIFGLK
                     GEITYSRAPLEEALERVGRRENGFLGRLFTGAAREIAASREETFSKIWEKQVELIKKG
                     PQGEILDEKDIKQLAGLGGRLGYLDVDMQERTLLLYLEQLELSISRLRGEMRERCRLS
                     TALGMMGSLLLVILMY"
     gene            complement(7210..8181)
                     /gene="spoIIIAA"
                     /locus_tag="ACZPCG_04490"
     CDS             complement(7210..8181)
                     /gene="spoIIIAA"
                     /locus_tag="ACZPCG_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HIR1284368.1"
                     /translation="MGKREEIAVLFPGTLREFIESCSLDFESMREIRLRAGRPVLLMY
                     GNREYGFTEKGEPVELEPDNQKGLFTLTERQIRETAEIMGGFSLYAAQEELRQGFFTV
                     RGGHRIGVAGRTVLNQREVGVLKSISSLNVRVAHEVKGCADQIMPLLCENGLFLNTVI
                     VSPPGCGKTTLLRDIIRQLSNGCREKSTGRRLFGITVGVVDERSELGACFQGVPQNDL
                     GMRTDILDGCPKSQGMMMLIRSMAPAVIAVDEIGGKEDIRAVEYIRSCGCGLAAAVHG
                     VSLKEVEKRPGVGELFKEGTFKRLIFLDQAGGAGHICAVYDGAGRLL"
     gene            complement(8272..8751)
                     /gene="rlmH"
                     /locus_tag="ACZPCG_04495"
     CDS             complement(8272..8751)
                     /gene="rlmH"
                     /locus_tag="ACZPCG_04495"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HIR1284369.1"
                     /translation="MKITLITVGKIKERFFEAAIEEYSKRLSRYCKLEIIQVADEKTP
                     DGASEALERQIREKEGERILSHIRDGAYVIALAIRGKELSSEELAAKIARLAVEGKGQ
                     LVFIIGGSLGLSEAVMKRSDYHLSFSPMTFPHQLMRVILLEQIYRSFRINAGEPYHK"
     gene            complement(8748..9245)
                     /locus_tag="ACZPCG_04500"
     CDS             complement(8748..9245)
                     /locus_tag="ACZPCG_04500"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1284370.1"
                     /translation="MKEIQIRQAVIEDLEQVAAVEARCFPAAEAADEKSLKERLEAFP
                     GSFLVAECEGRIAGFINGAATNERTIADEMLEDVSLHDPRGAYQSIFGLDVDEDFRHQ
                     GLAGRLMKALIEKAESEGRKGLILTCKEHLIGFYEQFGYCSMGVSKSVHGGAVWYDMI
                     LEFSR"
     gene            complement(9251..10084)
                     /locus_tag="ACZPCG_04505"
     CDS             complement(9251..10084)
                     /locus_tag="ACZPCG_04505"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HIR1284371.1"
                     /translation="MRQTSISGTARMLDPILELRGGSVYYPGGKQALKQVSAAFYPGE
                     KTAVIGNNGAGKSTFFQTLNRLVPLDEGELYCHGRLCTGSRKDILELRKCVGIVFQDP
                     DRQIIASTVEGEISFGLFNLGYSEDKIRYLTEQIIQRMGLTHLAHRPPHFLSGGEKKQ
                     VSISGVVVMKPEILLFDEPTASLDWKNTQLFKEQLKKLEQEQITQVISTHDMDFVWEW
                     ADRVLVFSEGELLADDRPEHIFGDEELLKRASLRQPVLYQLPGHPKTIEEWKQLQEKE
                     G"
     gene            complement(10081..10842)
                     /gene="cbiQ"
                     /locus_tag="ACZPCG_04510"
     CDS             complement(10081..10842)
                     /gene="cbiQ"
                     /locus_tag="ACZPCG_04510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HIR1284372.1"
                     /translation="MALHHRNHCHGNQILLMDQYAVCSQMASWNPDWKIMLGLISLLG
                     CIGSKTGLISVAVGLCMAVLTHKWGGVEWTVYGRMLTIPLFFVILSGAVIALDFTKEA
                     VMEAVFVTVKVLTGTACLYMISLTTPVYEVVGTLRRWHLPEVMLELMILMYRFLFGLL
                     ELYEQMKQSAQARLGDIGIRQGYRSFFGICANLLVLAFRRASDSFNAMEARGYDGELR
                     FLEEKKLVTGRQRLGIVLYGVLLAAGLLAERMWSI"
     gene            complement(10845..11174)
                     /locus_tag="ACZPCG_04515"
     CDS             complement(10845..11174)
                     /locus_tag="ACZPCG_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006783085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR1284373.1"
                     /translation="MKKPMWIAGLLVACVILTIVPFIAAPKDAGFGGADDAAGELVGE
                     IDPEYEPWAEPVLERILGGELPGETESLLFCLQAALGSGVICYGFGYLVARKKYSTND
                     TEERQEN"
     gene            complement(11174..11920)
                     /locus_tag="ACZPCG_04520"
     CDS             complement(11174..11920)
                     /locus_tag="ACZPCG_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HIR1284374.1"
                     /translation="MNVRKKGTAMLAWGILFSFAAASPAYAMHIMEGYLPMGYCIAWG
                     VVCIPFVVLGVKRINRLIKEHRKLLLIFAMAGAYAFVLSALKFPSVTGSSSHPTGTGL
                     GAILFGPTPMAVIGFLVLIFQALLLAHGGLTTLGANTFSMAIAGPVVSWGVYKLGRKI
                     KLNQKVAVFLAAALGNLFTYCVTSVQLGLAHPAADGVWSAVVRFLGIFALTQIPLAVI
                     EGLLTVVILIGLQTYAGPELRELGFGEENR"
     regulatory      complement(12067..12295)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(12327..13037)
                     /locus_tag="ACZPCG_04525"
     CDS             complement(12327..13037)
                     /locus_tag="ACZPCG_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1284375.1"
                     /translation="MARVLVVDDEKLIVKGIRFSLEQDGFEVDCAYDGEEAIELAKKT
                     EYDIVLLDVMLPKHDGFEVCQAIREFSDMPVIMLTAKGGDMDKILGLEYGADDYISKP
                     FNILEVKARIKAIIRRSLKAKRQKREEPSSMIGSGDLKMDTEGRRVFIGEKEINLTAK
                     EFDLLELLVRNPNKVYSREALLNYVWGNRAMDSGDVRTVDVHVRRLREKIEPSPSDPR
                     YVHTKWGVGYYFGVSNRG"
     gene            complement(13096..13839)
                     /locus_tag="ACZPCG_04530"
     CDS             complement(13096..13839)
                     /locus_tag="ACZPCG_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003523056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR1284376.1"
                     /translation="MKELKKHKQGRIILLLLGMFLAGILYSCSQPSPGTEVSLEYSED
                     GERREKVFSEPEASFSDETADCPGKEIAFSEAKVASEAENPEEEQIWIHVCGQVAAPG
                     VYSLNAGSRVFQALEAAGGVLEQGDGSILNLAMVLEDGMRIEVPDRTEAERLKTQGIL
                     SDQAFVTDPGDGFSGSPGKSASTGRKVNLNKAGAEELMTLTGIGKSRAEAIIQYRQEV
                     GAFRSIEDIMNVSGIKESAFEKIRDDITV"
     gene            complement(13930..14358)
                     /locus_tag="ACZPCG_04535"
     CDS             complement(13930..14358)
                     /locus_tag="ACZPCG_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003510229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR1284377.1"
                     /translation="MTGWIIALLSGALMSVQGVFNTEVTKQTGVWVAAGWVQATALLT
                     CVVIWLFTGRQPVSGLMGVTPKYVLAGGVIGALITYTVIRSMAALGPARTSLLIVVAQ
                     IIVAYLIELFGLFGVEKAPFAWNKLLGAAIAVGGIIIFQR"
     gene            14602..15996
                     /locus_tag="ACZPCG_04540"
     CDS             14602..15996
                     /locus_tag="ACZPCG_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006776979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1284378.1"
                     /translation="MTANNEPAADLTEKDRAFRAFVREENMWKVVFYVCTPLALYQSL
                     NQIFKIFDSMMASHIGASSVSAVAYLSQLNLMLSALGGGLAVGASIKVSEAYGAGDFK
                     LVKRRVSTLFALCGILGADILLILVPFAPQFLRLARTPEEFITEGTTYFILDLFGIVL
                     NFFNSVYIAIERARGNSQRILHLNMGVIVVKLALTALFVYVFHSGINMIAVATIISQL
                     LMFSAAIYHLSRKGNAFSFSLRSISLKRRVVRPMVLLSVPVIIEKMAFSFGKVVVNSM
                     CTIYSPLTVGALGISNNLGGITTNPQNGFQEGGSAIISQSLGAGLPKRALEAFRCILI
                     INMAIGAVLMSLTLIFLGSISRLFSGGDPQFQQMICSIYQMEALGSIPLGINAAVMAL
                     LYGFGKTRISLIINFSRVFIFRVPVLWGLQQFTNLGDISAGIVMAVSNIATGVLAVIV
                     GWITIRQIKKQYGI"
     gene            complement(15993..16538)
                     /locus_tag="ACZPCG_04545"
     CDS             complement(15993..16538)
                     /locus_tag="ACZPCG_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5662 family protein"
                     /protein_id="HIR1284379.1"
                     /translation="MKLINIWNHFKTITKHRMLVCRHCFQIGLYRQGLTHDLSKYSPE
                     EFFTGVRYYQGTRSPNAAEREDLGYSKAWLHHKGRNKHHYEYWIDISVRKEEGLVGNK
                     MPLRYVAEMICDRIAACEVYKGKAYTSAAPLEYYEFTKKYITIHPETRALLERLLIML
                     KDRGEKETFRYLRELLKQGDY"
     gene            16694..16891
                     /locus_tag="ACZPCG_04550"
     CDS             16694..16891
                     /locus_tag="ACZPCG_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006776951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HIR1284380.1"
                     /translation="MKGTVKWFNNQKGYGFISDEQGNDVFVHYSGLNMEGFKSLDEGA
                     EVEFDVVNGAKGPQATNVTKL"
     gene            complement(17029..17910)
                     /gene="hslO"
                     /locus_tag="ACZPCG_04555"
     CDS             complement(17029..17910)
                     /gene="hslO"
                     /locus_tag="ACZPCG_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HIR1284381.1"
                     /translation="MADYVIRATAAEGQIRAFAATTRDLTEAARQAHNTSPVATAALG
                     RLMTAAVMMGYDMKGDADLLTLKIQGDGPIGGLVVTADAHGGVKGYAFHPEVMLPPND
                     KGKLDVGGALGLGVLSVIKDIGLKDPYVGQTILVTGEIAEDLTYYYATSEQTPSSVAL
                     GVLMNKNNTVRQAGGFIIQLMPGASDAVIDALENKIKEIHSITTLLDVGNTPEMILEY
                     ILGEFGLEINDKLPARFYCNCTKDRVAKALISVGKKDIQEMIDDGKPIEVNCHFCNKN
                     YVFDVEELKELLELARS"
     gene            complement(17913..18677)
                     /locus_tag="ACZPCG_04560"
     CDS             complement(17913..18677)
                     /locus_tag="ACZPCG_04560"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002603751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HIR1284382.1"
                     /translation="MEAYTSFAQVYDLFQDNVPYEEWAAYLKELLKEYGAEDGLVLDL
                     GCGTGSMTQLLAEAGYDMIGADNSEEMLEIAMEKRAAAGLDILYLLQDMREFELYGTV
                     RAVVSICDSMNYIMEPEELTQVFRLANNYLDPGGVFIFDLNTEYKYKELMGENTFAED
                     RTESSFIWQNYYDEKERINEYDLTLFIREGELYRKFEETHFQRCYSLDEVKAAAEAAG
                     MEFVTAYDAFTKDPVKEDSERIYVILREKGKTEKGR"
     gene            complement(18754..19263)
                     /locus_tag="ACZPCG_04565"
     CDS             complement(18754..19263)
                     /locus_tag="ACZPCG_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008718136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HIR1284383.1"
                     /translation="MALLETWRNLAYGDGLDDKKKEELWAGYFQIEKGIYEQILSAPT
                     EVITGTVKELAEKYNTELLIMTGFLDGINESLKGYENPIDTMEEDTEVKIEIDPEKLY
                     YNMVEAKANWLYELPQWDSILSEEKRKELYKAQKASGTIRKEKKIFPNDPCPCGSGKK
                     YKKCCGKNA"
     gene            complement(19531..19704)
                     /locus_tag="ACZPCG_04570"
     CDS             complement(19531..19704)
                     /locus_tag="ACZPCG_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002575716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HIR1284384.1"
                     /translation="MKVNGTNECIQCTIKNCAYHADTTDYCTLDTIKIGTHEMNPTKK
                     ECTDCESFVNKMQ"
BASE COUNT         4839 a         5693 c         3652 g         5537 t
ORIGIN      
        1 tggattctgt atcatccgtc atataaaggt taatccccaa ccgcaaagca tactgcttca
       61 tttgctcatg cctttcaatc tcttttgggt ttctaatctg ggaacggtca accgtcctct
      121 taggggacaa ctgaatcaac ctgtctattt cctttaaatg cacatctatt ccctttaatt
      181 ctttgacatt catcattttt taggtgcccc tttcttatat attatatgat attcactggc
      241 tattttcagc atatcattga ttgctctagt tccattattt attatgccct tttccagata
      301 ttcattcgcc aatctggcat aatcgcctat gaaccgttca cattccgatt tataagttct
      361 ttctatccta ttttcacata atctggaaaa gaaggatttg tgagaatgta tattacacct
      421 tttttatcac tcatatactt tttctccatt tatcattttt tcaaagtgta atttctgctt
      481 atcgccaaag tgtcaatttg atactttaga atgaactttt gaacctttag aattactttt
      541 aatacacaat cctaaaataa tccagatacg ctttatattt atcctgtgcg gtcaggcagg
      601 tatctgtcag ataacctttc tcattgttcc attctttcaa tccacgataa tagaacattt
      661 tcagattatc ttcgataatg aacggaacga tattatattt caggcattcc ttaaacataa
      721 tcaatctgcc cacacggcca ttgccatcct ggaacggatg gattcgttca aacttcacat
      781 gaaaatccag aatatcctca aaagtctttt cttcttttcc gttgtactca gtcaacagag
      841 ctttcatttt atcggcaact tcttccggaa gtgctgtttc cattccgcca acttcgttcg
      901 gcatcttttt ataatcgcct acagcaaacc aatcttttct ggaatcactt gtgcctgttt
      961 taagtgtgtt gtgcaattct ttaataaact tttcggtaag tgcacctttt gcattatcaa
     1021 taatcatatc aatgcaacgg aagtgatttg ctgtttcaat cacatcatcc acattcagca
     1081 cttctttttc tacaccgatg gtgttggttt caaagatata tctggtctgg tcatgggtca
     1141 gactgctgcc ttcgatatga ttggaattat atgttaaatc aatctgtgtt ttatggtaaa
     1201 tgcctccgga atacttgctt accttctgct ccttcagaat atccagtaag gtaatcggct
     1261 gctctttctt tttattggaa cgctccggct tttctgcatt ttcaggaata ttccaggtct
     1321 tgccggtaag aaacgcaccg tctactcgcc catgagcgca gtaatttcga acgcttcgtt
     1381 ccgacacatt ccattttttc gctatttcat taactgaacg atattgcatc catattcctc
     1441 cacttttcta acgcttaatc aagttaatcc tttatgtccg tcaaattttc catcttaaac
     1501 acataatacc cttcatagta ataactgtga tcgatgccct tcatataaat ttctcgatca
     1561 tttacattat tcgtaagtgc atttttcaga agaaacttta tttcaatatc tttgatagga
     1621 cttcgctcca tggcaagcag ataatccact ttatccacct tgctccagtc aaccacatag
     1681 ccgatttctt ttttcaacat caggtcaagc catattctcg tacttctccc atttccctct
     1741 cggaacggat gagccacgtt catttcaaca tatttttcga caatttcatc aaaagttgac
     1801 tgtggcattt tgtctatact ctgtaacgct gcttccaaat acatcagagg agcaaatcta
     1861 aaatttcctt ttgaaatatt aacctttcgt aatttaccgg caaaatcata aatctcannn
     1921 nnnnnnntat tgatggatca tcgcgagact tttataagta cctagttcta atgtctcaaa
     1981 aaagccactt tcaaacattt caagcgcttt ttgcttactg attttttctt ccattcttgc
     2041 taattctgta gaatctgtaa tacctaattt attttcaagc atagtttacc tcacattctt
     2101 catgcgatca cttttccagt cacgaatcta tctttataag tataccataa tatcggcaac
     2161 aaatctattt tatcatgaaa tatcttactt ttattttgcc gcatttaata gttctttttc
     2221 ctcttaacgc aaaaatggca aagttcttat cgacaacact tcaccatcag atgttggtaa
     2281 ataaaatatc ctataaataa gaaagaccca ccgggttgcg catgaaaatc agaggcgtgg
     2341 tgggttctgt taagtttaac atacagtttt tgaagttact gtcaatattt cttttcaaaa
     2401 aaaacgataa ctctccatcc gcaccgaata tctacacaaa aaaagcccaa cagaaattcc
     2461 tttccgtcag gctgtctttc ctatacacat ctattttatc atactgctgt caacctcttt
     2521 ttctcctact cctccatatt caaaagcgta ataatgatct ggtcggctcc cacctctgtt
     2581 ttccgtttca caatatcctc aatctgagcc ctctgcggat ctgtgatcga tggggcattg
     2641 atcactacat ctactttttc atctgcaatg cttactaccg gatcggagaa gccttttgcc
     2701 atgagaaggg tctccgcagc attttccttt tctgaaatct ctgtcaggcg gatcatatcc
     2761 tgaatggcct gctgcttggc tgcttcatca atggaggtac tgttgatcag gctcatcagg
     2821 gtttccttat ttctggctcg aacctgctcc ctgcttaact gaactcctgc tatgtagtcc
     2881 gccacgctca tgccgctggt aagcactgcc tctccgggat tttcaatccc tgcgggagca
     2941 tcctgtgcat ctccggcttc tgaaacagca tcctgtccgc ctttctctgc cagagccagc
     3001 tgctctgtct ccgcttccat gttctctccg gcctcggcca gctgctctgc cgcttctgcc
     3061 cctacttctt ctttctgagc caggccttca ctggggagtg cttcctccac ctgatccgaa
     3121 tcctgatcca gacttccgat ctccaaaagg tcatcaccgg cggccttatt ttccgccagc
     3181 agatcttcat cggaaatatc catcatcccg gcttcataga tattgcctgc tgcggcaaga
     3241 tcgcgttttc ctgcataatt gagatatcct gccgcagcaa tcattacagc cagtgtggtg
     3301 ataataatct ggtttctgcg gaacagccgt ttcattttta catccttcat atgtgctgtg
     3361 acctgtttta cctgtttcat gttcctgatc ttcatgtcct gcactccttt tttattccac
     3421 cctctttaat actttaatct tatgtgccgg aagccccaat aatgcttcca ttgcctggga
     3481 aatttcagaa cagacctccg gactccctcc tccgtcggcg ctgacgacga ttcctgaaat
     3541 ttcaggacat aattcctttt ccaccactgg tccgtttgac ccgcctccgg actgaggcag
     3601 gactgtgctt tcctccgact ggctctctgt aatctcacgg cttccgcctg cagagtccgt
     3661 ttcctttgtc accgtgctgc tgccggaacg atccaccctg agtaccttct ggcctgtgga
     3721 ttttaacact accattacat ccacctgtcc aaccccatct acagaccgta acagttcccg
     3781 tatccgcttt tccaaacgtt cttcataaga gcgcgaagaa aaagagctgc tgatatccca
     3841 tgaaaccaaa ctgtgttctt ccgtccccgc tgcctgttct ccgctcttcg gccgatcttc
     3901 ttttacagtc tttttctctc ctcccggaac ggataaaagc atgagaaata cacctgcaag
     3961 cagaaacagc agccattttt ccctagtcat ttttccattt aattttaaca gcttctcctt
     4021 ccagcccata atacgtttct acctttcctt ttagcgtctc tgccgcctgc ttctcccttt
     4081 ctgaaagctc ctgcttcaca ccctccttat cctctctttt ctcccccgcc ccgatttcaa
     4141 tgcggccgat actcaccgga cgttcttctg atatagggga taaaagcatc tgcccggctg
     4201 cccggatatt ttcccgtcca gaaccttcct gtccggcccc ctcttgtgcg aaaccttcct
     4261 gttcaaaaaa atcttccccc gatacttcaa tctcaattgc caaaatcttc ccgtaggagg
     4321 aactttcctt attttcctcc atctgtacgc ttacctgaga gcagacaatt ccctgtgcct
     4381 tgaccaatgc tgttatctcc ttttccacat tttccctgta ctgccccgtt atgcccgcca
     4441 gacgcttttc ttccattcca aaaatttctt ttttaagctc ccgtgactcc tgtctgaacg
     4501 taaactgatc aaacagactg cttacctgtt tttcccaatc ccccaatgag ccaaaaggcc
     4561 ccaatacaac aagcacaaga accagccctc caaataaacg cagatatcgt ccataggagc
     4621 ttccggccaa aaggtgctct gccagtgaaa gaaagaccat atagtaaata atatttctgg
     4681 cccagcctaa aagagcctcc gccataaccc ctccctttta tctctgcttc ccctccttct
     4741 atgccgtcct gtcaggcaga cagataggcc acatccgctg ccttgcaggt aacagcaatg
     4801 gcgatcacaa acagaagcag agaatataaa acaatcttca gcaacagcct gtgtcccctg
     4861 gaaacaccga tcacacagga aaccattctc ttatcacaca ccggctgcat gacggctcct
     4921 gccgcctgat agaacagtgc aagtgtaaga agctttgccg ccggaagaaa agcaatgagt
     4981 gccaaaactg tcacacctgc ggctcccatg gtatttttga tcaatacacc tgaacctaat
     5041 accatccggg ccgccgctcc tgcaccggct cccagccccg gaacggcctc tgccagcttt
     5101 aaaaggccgg aacggcctac actgtctgca tagggcagaa ccatggcttc cagcacatgg
     5161 aggcccaaga tcagaccaaa catggttttt atgccccatt ccactgcctg ttctgtcaaa
     5221 gatgtcattt tcgacagcag cggctccttc atcacatggc ttcccaaaac cagaagcaca
     5281 taaactctga ctccggtaag aaatacgctc tttgacagcc actgaacaca aaagacagcc
     5341 gccatcatag cctcatacat acagattccc gttatgctcc cccctgaaaa tgacactgcc
     5401 ataaagtaag ccggtatcag cacacccata aactccagaa tccggtccaa aacctctgcc
     5461 gcaatggaaa gacttgtcag aaaactgcct gccagacagg caaacagcaa aaggtatgcc
     5521 acataaaatc cggtatctga gatctgcccg tttttaaaaa cagaagccgc cccggaaaac
     5581 atggctccaa gaagaccaat agccgctgcc tgagtcaaaa gcccggctcc ttcccttacc
     5641 tgtgaaaata aaagctgtcc gaatccctct tttatggctg ttcccagcgc tcccagattc
     5701 cctgacgcaa aagccttcat cagatccgta aataaaagat ccactgttcc gttttttcct
     5761 cccaacgatc gatcaagata cttctgcagc tgctccagct cctctgaaat ccccatatca
     5821 tgagcagcct gtccaatctc catttcttca cttgtctctt tattattttc aaaattttcc
     5881 ttttcataaa aagcgcccgc cgttatcgtg ctccctctgc cgcttatcca cagagccatc
     5941 aaaaaaacta tcagttttag accgattcgt ccatatttta ctgcctttct tccctgtgcc
     6001 atagcagctc tccctccccg atccagcaaa atcacagtat tctgccatac tatcccataa
     6061 actgatccag tatttcaaag agagccaaaa aaacaggcat acttacccca agaatagaca
     6121 gctttccgaa aatctcgatc tgatttccca aagcgctgta gcccgcgtcc ttacagattc
     6181 cggaggcaaa ctcagccaca tatgtgatcc caaccatttt cagaagagca cccagatagg
     6241 ccggatcaat tttgatatat ccttccagac gtctcatggc atctaaaatc acctccagac
     6301 gtccggtacc gcagatcata aagagtatct gtgctcccag caccatataa agggcatact
     6361 ctgcccggac tcccttcagc catacggcaa gaaggactgc cgttataccg gcggccccta
     6421 tggtaagaat actcatggct cctcctacag caaaaacaaa cgtttcatgg tttcaaacag
     6481 atcatatatg tagggaacca tccagaacag taccagaacc agacctgcca gactggtgag
     6541 aaatgcctgc tcatcccggc cgctgtgctt tagtacctgg cagattacag acaccaggat
     6601 tcccacagcc gcaatgcgga aaatcagatt tacatccatc gtttctcccc tttcctccgt
     6661 cgcagctaat acatcagaat cactaaaagc aggcttccca tcattcccag agctgtgctc
     6721 agacggcatc tttccctcat ttccccccgc agacggctga tagaaagctc cagctgctcc
     6781 agataaagaa gcagcgtccg ttcctgcatg tccacatcca ggtatcccaa acgtccaccc
     6841 agaccggcaa gctgctttat atctttttcg tccagaattt ccccttgagg cccctttttt
     6901 ataagctcca cctgtttctc ccatatttta gaaaatgttt cctcccggct tgcggcaatc
     6961 tccctggccg ctcctgtaaa taaacggccc aagaatccat tttcccgccg tcctacccgc
     7021 tccagagcct cctccagcgg agctctgctg tatgtgatct caccctttaa gccaaaaatc
     7081 atctgtctaa gcgtttccag agcctccagt ctggccctcc atctccaggc catccacatt
     7141 ccccagccag taccggaaaa cagtaccagc ccaattccgg cccattttat aaccatgctc
     7201 cctcctcctt tacaggagcc ttcctgcccc atcgtacaca gcacagatat gacctgctcc
     7261 cccggcctga tccagaaaaa tcaggcgttt aaaggttcct tccttaaaca gttcccctac
     7321 ccccggccgt ttttctactt ctttcaggga tacaccgtga accgcagctg ctaacccgca
     7381 gccgcagctt cggatatatt ccacagcccg tatatcctct tttccgccaa tctcatccac
     7441 tgctattact gccggagcca tggagcggat cagcatcatc atcccctggc tcttgggaca
     7501 gccatccaga atgtccgttc tcattccaag atcattttgg gggactccct gaaagcaggc
     7561 ccccagttcc gagcgctcat ccaccacgcc gactgtgatt ccaaaaagac gccttcccgt
     7621 gcttttctcc cgacagccgt tggacagctg acggatgatg tcccgaagaa gggtcgtttt
     7681 tccgcatccc ggcggcgata cgattaccgt atttaaaaaa agcccatttt cacataaaag
     7741 aggcatgatc tgatccgcgc atccctttac ctcatgagcg acgcggacat ttaaagagga
     7801 gatagatttt aagactccca cctctctctg gttcagcacc gttcttcctg ccacaccgat
     7861 ccggtgtccg ccacggacgg taaaaaatcc ctggcgcagc tcctcctgag ccgcatacag
     7921 agaaaaacct cccatgatct ctgcagtctc ccggatctgt cgctctgtaa gcgtaaaaag
     7981 gcccttctga ttgtccggct ccagttccac cggctctcct ttttctgtaa atccatattc
     8041 cctgtttcca tacatcaaaa gcaccggacg gcccgcccgc agccgtatct ccctcatgga
     8101 ttcaaaatcc agagaacagc tctcaataaa ctcccgcaag gttcccggaa ataaaaccgc
     8161 gatttcttct ctctttccca taccatctct ccctttatct caaatatatg aaacaggcct
     8221 gtttttatga cagaatcact ctgccgtaaa aacaggcctg cagacaaatc tttatttgtg
     8281 ataaggctcc cccgcgttga tccgaaagct tctgtaaatc tgctccaaca agatcacccg
     8341 catcagctgg tgaggaaatg tcatgggcga aaagctcaaa tgatagtctg agcgtttcat
     8401 cacagcttct gaaagcccca gagaaccgcc gatgataaac accagctgtc cttttccctc
     8461 cactgccagc ctggcaatct ttgcagccag ctcctcactg gaaagctcct ttccccggat
     8521 ggcaagagct ataacgtatg ccccatcccg gatatgagaa aggattctct ctccttcctt
     8581 ttcccggatc tgccgctcta aagcttcact ggcgccgtcc ggcgtctttt catctgccac
     8641 ctggatgatt tccagcttgc agtaacggct taagcgcttg ctgtattcct caatagcagc
     8701 ttcaaaaaag cgttccttta tctttcctac cgtaatcagt gttatcttca tcggctaaac
     8761 tccaatatca tatcgtacca cacggctccg ccatggaccg atttcgacac gcccatgctg
     8821 cagtatccaa actgttcata aaaaccgatc aaatgctcct tacaggtaag gatcaggcct
     8881 tttctgccct cactctccgc cttttcgatc aaagccttca tcaaacggcc tgccagtccc
     8941 tgatggcgga aatcttcatc cacatccaga ccaaagatac tctggtatgc tcccctggga
     9001 tcatgaagag atacatcctc aagcatttca tccgcaatgg ttctttcatt tgttgccgcc
     9061 ccattaataa accctgcgat ccttccctca cactctgcca ctaaaaaact gccgggaaat
     9121 gcttcaagcc tctccttcag acttttttca tccgctgcct ctgctgccgg aaaacagcgc
     9181 gcctctactg ctgccacctg ctctaaatcc tctatcactg cctgtctgat ctgtatctct
     9241 ttcatccctc ttatccttcc ttttcctgta actgtttcca ttcctctatg gtctttggat
     9301 gccccggcag ctgatataaa acaggctgcc tgagagaagc ccttttaagc agctcctcat
     9361 ccccaaatat atgctccgga cgatcatctg ccaaaagttc tccctctgaa aacaccagaa
     9421 cacggtctgc ccattcccac acaaaatcca tgtcatgggt ggaaatgacc tgtgtgatct
     9481 gctcctgctc cagtttctta agctgttcct taaaaagctg cgtatttttc cagtcaagac
     9541 tggcagtagg ttcatcaaac agcaggattt ccggcttcat taccacaaca ccggaaatac
     9601 tgacctgttt cttttcccct ccgcttaaaa aatgaggcgg acgatgggca aggtgagtaa
     9661 gccccatccg ctggatgatc tgttcagtca gatagcggat tttatcctct gaatagccca
     9721 gattaaagag tccaaaggaa atttccccct ctacggtcga agcgatgatc tgccggtccg
     9781 gatcctgaaa tacgattccc acacactttc tcagctccaa aatatccttc ctgctgcctg
     9841 tacagagcct tccgtgacag taaagctctc cctcatccag aggtacaagc cggttaagag
     9901 tctggaaaaa cgtagatttt cctgctccgt tattcccgat cacagccgtt ttttctcccg
     9961 gatagaaggc cgcactgacc tgttttaatg cctgctttcc cccgggataa tacacactgc
    10021 ccccgcgaag ctccaaaatc ggatccagca tccgggctgt gccgcttatc gatgtctgcc
    10081 tcatatgctc cacatcctct ctgccaaaag ccctgctgcc agaagcactc catacagaac
    10141 aatacccagc ctctgccttc ctgtcaccag ctttttctct tccagaaaac gcagttctcc
    10201 gtcatagcct cttgcctcca tggcattaaa gctgtccgat gcccgccgaa aggccagaac
    10261 cagaagattg gcacatattc caaaaaatga acggtatccc tgtcttatcc cgatatcccc
    10321 cagcctggcc tgagcactct gcttcatctg ttcataaagc tctaaaaggc caaacaaaaa
    10381 gcggtacatt aagatcatca gttccagcat cacttccggc agatgccatc ttctcaaagt
    10441 tccaaccact tcatatacag gcgttgtcag acttatcata tacaaacagg ccgttcccgt
    10501 caatactttc accgtcacaa aaacagcttc catcacggct tcctttgtaa aatccagtgc
    10561 aattacagct ccgctgagga taacgaagaa aagaggaatg gtcagcattc tcccgtaaac
    10621 cgtccactcc actcctcccc atttatgggt aagcactgcc atgcacagcc ccactgccac
    10681 tgaaatcagg cccgttttgg atccaataca ccccaacagc gaaataagtc ccagcatgat
    10741 cttccagtcc gggttccatg acgccatttg ggagcacacg gcatattgat ccatcaaaag
    10801 aatctgattt ccatgacaat gatttctgtg atgcagcgcc ataattagtt ctcctgtctt
    10861 tcctctgtat cgtttgtact gtatttcttt cttgccacca gatatccaaa tccatagcag
    10921 atcacgccag atcccaaagc tgcctgaaga cagaacagca ggctttccgt ttctcccgga
    10981 agttctcctc ctaaaatccg ttccagaaca ggctctgccc atggctcata ttcgggatcg
    11041 atctcaccta ccagttcgcc ggctgcatca tcggctcctc caaatcccgc atccttggga
    11101 gcagctataa acgggacaat tgtcaatatt acgcaagcca ccaaaagacc tgcgatccac
    11161 attggtttct tcattatcga ttctcctccc caaatccaag ctctcgaagt tccggacctg
    11221 cataagtctg cagaccgatc aatatcacaa ccgtcagaag tccttcaatc actgccagcg
    11281 gaatctgtgt caatgcaaaa attcccaaaa accgtacaac agcgctccat acgccgtctg
    11341 ccgcaggatg ggccagtccc agctgcacac tggtcacaca atatgtaaac agatttccaa
    11401 gggctgctgc cagaaaaaca gccaccttct gattcagctt tatttttctg cccagcttat
    11461 aaacgcccca gctgacaaca ggacctgcaa tcgccatact gaaggtattg gctcccagcg
    11521 ttgtcaaacc tccatgtgca agcaaaagcg cctgaaaaat cagcaccaga aagccgatca
    11581 ctgccatggg agtcgggcca aagagaatgg ctcccagacc ggttcccgtt gggtggctgg
    11641 aggatccggt cacacttgga aatttcaggg cagacagcac aaaggcatag gcgcctgcca
    11701 ttgcaaagat taaaagcagc tttctgtgct cttttatcag acggttgatc cgcttcactc
    11761 ccaataccac aaagggaatg cagactaccc cccaggcaat acagtacccc atcggaagat
    11821 atccctccat aatgtgcatg gcatatgccg gtgacgccgc tgcaaaactg aagaggattc
    11881 cccaggccag catggcagtt cctttttttc ttacattcat tgatgtatgc tcccttctta
    11941 aaagtaaaaa agcctgtttg ccacatagtt tctacgtgat ccaaacagac atgataagaa
    12001 agcggatgct ccgccttcct cccttttcag ggtatctatt ataaaaagct gtgctccgca
    12061 gcctttataa tcacgccgaa aaaggcaggt cttctggctc aggcatcctc atcaccggct
    12121 ccttcccaca tagattcatg cagtggcgtt tcgcaaaagc ctgtttctat tttaacagct
    12181 ttctgcatcc ggtaactctt cctttacagc ggcgggaccg catgggcttt ccacccattt
    12241 ccctattatc ccggacttcc catggaatcc gggcaccttt ttctgttctg ttttccattt
    12301 tccctcagca ggcagggatc gaggctttac cccctgtttg atactccgaa ataatatccc
    12361 acgccccatt ttgtatgtac atatctgggg tcacttggac ttggctcgat cttttctctc
    12421 agacggcgca catgaacgtc taccgtacgc acatccccgc tgtccatggc tctgtttccc
    12481 catacataat tcaaaagcgc ctcacggctg tataccttgt tgggattgcg taccagaagc
    12541 tctaagagat caaactcctt tgcagtaagg ttgatctcct tttcaccgat aaatactctg
    12601 cgcccctctg tgtccatctt taaatctcca gaaccgatca tagaagaggg ttcctcccgc
    12661 ttctggcgct ttgccttcag gcttcgccgg ataatggcct tgatcctggc ctttacctcc
    12721 agaatgttaa agggcttgct gatgtagtcg tccgccccgt actccaatcc caggatctta
    12781 tccatatcgc cgcccttggc cgtcagcatg atcacgggca tgtcggaaaa ttcccgaatc
    12841 gcctgacata cttcaaaacc gtcatgcttt gggagcatca catctaacag cacaatatca
    12901 tactctgtct tttttgccag ttctatggct tcctctccgt cataggcaca gtccacctca
    12961 aaaccgtcct gctccagact gaaccggatt ccctttacga tcagtttttc atcatctact
    13021 accaataccc gagccatgtt tcattttcct tcctgtctgt ctatttaaaa atgccctgac
    13081 gggcgcagtt ttcttttaaa cggttatatc atcccgtatc ttttcaaatg cactttcctt
    13141 gatcccggat acattcataa tatcctcgat cgagcgaaaa gcccccactt cctgacggta
    13201 ctggataatc gcctccgccc tggattttcc aatgcctgta agtgtcatca gctcctctgc
    13261 accggcctta ttcagattca cttttctgcc cgttgaagcg cttttcccag ggcttccgct
    13321 aaaaccgtca ccgggatccg tcacaaaggc ctgatctgaa agaatcccct gagtcttcag
    13381 gcgctctgcc tctgtccgat ccggaacttc gatccgcatc ccgtcctcta agaccatggc
    13441 cagattgaga atactgccat ctccctgctc aaggactcct ccagctgctt ccaaagcctg
    13501 aaatacacgg cttcctgcat tcagggaata cactcccgga gccgccacct gaccgcagac
    13561 atggatccag atctgctctt cttccggatt ttctgcctcc gaagcaactt ttgcctctga
    13621 aaaagcaatt tctttccccg gacaatcagc agtctcatcc gaaaaagagg cttctggctc
    13681 cgaaaaaacc ttctccctgc gttccccgtc ttctgagtat tcgaggctta cctctgtccc
    13741 cggcgatggc tgggagcagc tgtataaaat ccctgccaaa aacattccca ataaaagtaa
    13801 aataatcctt ccctgcttgt gcttttttaa ttccttcaac gatatctcct ccttttagag
    13861 ataccgtccc ggcctgctgt atctattcta tcggaaagcc ggtttaaata caagcgttat
    13921 ttcttaatcc tatcgctgaa aaatgataat cccccctaca gcgatcgcgg cccccaaaag
    13981 cttattccat gcaaatggag ccttttccac tccaaacagg ccaaacagct cgatcagata
    14041 agccacgatg atctgagcca ccacgataag aagagaagtt cgggccggcc ccagagccgc
    14101 catgctgcgg atcaccgtgt aggtgatcaa ggccccgatc acgccgcctg ccagaacata
    14161 ctttggcgtc acccccatga gaccggatac cggctggcgt ccggtaaaca gccagatcac
    14221 cacacaggtc agcagagccg tagcctgtac ccatcctgct gccacccata caccggtctg
    14281 ctttgttact tctgtgttaa atacaccctg cacactcata agagctcccg ataaaagagc
    14341 aatgatccat cctgtcatcc gtttttcctc ctgattgttt tatacaccac tgtttccaac
    14401 ggttctaaca gtcatttttc ccatcctctc tttccctata cacagattat ttgtatacgg
    14461 actttatttt ttgtatacaa atccgattgt tttctctgta tctctgggtt ataatccata
    14521 ggaatcccct gtttccgaca gaatctgtcc ggagttttcc ggacccgaca taaaaaaggg
    14581 gaagaaacgg agaacatgat catgacagca aacaatgaac cagccgcgga tctcacagaa
    14641 aaggaccggg ccttcagagc ctttgtacgg gaagaaaata tgtggaaggt ggtcttctat
    14701 gtatgtacgc ccctggccct ttaccagagc ttaaatcaga tttttaaaat ctttgattcc
    14761 atgatggcgt cccacatcgg cgcttcctct gtgtctgccg tagcctatct atcccagctt
    14821 aacctcatgc tttccgctct gggcggcgga ctggctgtag gcgccagcat caaggttagc
    14881 gaagcctacg gagccggaga tttcaaactg gtaaaacgga gagtcagcac tctttttgcc
    14941 ctctgcggaa tcctgggagc cgacattttg ctgatccttg tcccctttgc gccccaattt
    15001 ctgcgccttg caagaacgcc ggaggaattt atcacagaag gcaccaccta ttttatcctg
    15061 gatctttttg gaattgtact gaattttttt aacagtgtct acattgcaat cgaacgggcc
    15121 agaggaaact cccagcggat ccttcatctg aatatgggcg ttattgtggt aaagctggcc
    15181 cttaccgctc tctttgtata tgtattccac agcgggatca atatgatcgc cgttgccacc
    15241 atcatttccc agcttctgat gttttctgcc gccatctacc acttaagccg taaaggaaac
    15301 gcattttcct tctctttaag gagcatttcc ttaaaacggc gtgtggttcg gcccatggtg
    15361 ctcttatccg tacctgtaat catcgaaaaa atggcatttt cctttggaaa agtagtggta
    15421 aattccatgt gcaccatcta cagccctctt accgtaggag cactgggtat ttccaataat
    15481 ctgggcggca ttaccaccaa tccccagaac ggctttcagg agggcggctc tgccattatc
    15541 agccaaagcc tgggagcagg acttcccaaa cgtgccttag aggccttccg gtgcatcctc
    15601 atcatcaata tggcaatcgg ggccgttctt atgagcctga ccctgatctt tttagggtcc
    15661 atcagccgtc tcttttccgg cggtgatcct cagttccagc agatgatctg ttccatctac
    15721 cagatggaag ctttaggatc cattcccctt ggtatcaatg cggctgttat ggctctgctt
    15781 tacggatttg gcaagacaag gatttccctt atcatcaatt tcagccgtgt atttatcttc
    15841 cgtgtacctg tactgtgggg tctgcagcag ttcacgaacc ttggagatat cagcgccgga
    15901 atcgtcatgg ccgtgagcaa tattgccacc ggcgttctgg ccgtcatcgt aggctggatc
    15961 accatccggc agattaaaaa gcagtacggg atttaataat ccccctgctt taaaagttcc
    16021 ctcagatacc ggaaggtttc cttttctccc cggtccttca gcataatcaa aagcctttca
    16081 agcagcgccc tggtttccgg atgaatggtt atgtattttt tcgtaaattc atagtattcc
    16141 agaggcgctg cgctggtata agcctttccc ttatacacct cacaggccgc aatccggtca
    16201 cagatcatct ctgccacata ccgcagcggc atcttatttc ccactaatcc ctcttcctta
    16261 cggacagaaa tatcgatcca gtattcataa tgatgcttat tgcggccctt gtggtgaagc
    16321 caggcctttg aatagcccag atcttctctt tccgccgcat tgggactgcg cgttccctga
    16381 taatagcgta caccggtaaa aaactcctca gggctgtatt ttgacagatc atgggtcagc
    16441 ccctggcggt aaagcccgat ctgaaagcag tgcctgcata ccagcatccg atgttttgta
    16501 atggttttaa aatgattcca tatattgatc agtttcatta cgctctcctt taaaagttac
    16561 ttaaaaacag ccctgtatag accccggttt atgcacaaaa tgtttgacac ccggtaagat
    16621 ctgtgatatt ctaatggaag ctgtgaaatt acttgcagct taacattttt tttatttttt
    16681 tggaggtatc acaatgaaag gtacagttaa gtggttcaac aaccagaagg gttacggctt
    16741 catctccgac gagcagggca acgatgtatt cgttcactac tctggtctga acatggaagg
    16801 cttcaagtcc ttagacgagg gcgcagaggt tgagttcgac gtagtaaacg gagctaaggg
    16861 tcctcaggct accaacgtaa ccaaattata atcaatgaac aaacagtgta cctttttcaa
    16921 acatatatcg gttcgataga aagttgaagt aagttatatc tgaatttcaa atgattaccg
    16981 acaagagcca gtcgaaagac cggctcttgt tgtttttaat tcacaggact atgatctggc
    17041 cagctccaag agctccttta attcttccac atcaaacaca tagttcttat tgcagaagtg
    17101 gcagttcacc tcaatgggct tgccgtcgtc gatcatttcc tgaatatcct tctttcccac
    17161 gctgatcagt gcctttgcca cccggtcttt ggtacagttg cagtagaaac gggccggaag
    17221 cttatcattg atctccaggc caaattctcc taaaatgtac tccaagatca tctccggcgt
    17281 atttcccaca tccaaaagcg tggtaataga gtgaatctcc ttaatcttat tctctaaggc
    17341 gtcaattact gcatcagacg cccccggcat gagctggatg ataaaaccgc cggcctgacg
    17401 gaccgtattg ttcttattca tcaaaacgcc taatgccaca gaagagggcg tctgctcaga
    17461 ggttgcgtaa taataggtaa gatcctccgc aatctctcct gtcacaagga tagtctggcc
    17521 cacataggga tcctttaagc cgatatcctt gatcacgctt aacacgccca gtcccagcgc
    17581 tccgcctaca tccagctttc ccttgtcatt gggaggaagc attacctccg gatggaacgc
    17641 atagcccttt actccgccgt gggcgtctgc cgtcaccaca agaccgccga tggggccgtc
    17701 tccctggatc ttcagtgtga gaaggtctgc gtctcccttc atgtcatatc ccatcataac
    17761 agccgctgtc atcagccgtc ccagagcagc cgtagccaca ggacttgtat tgtgagcctg
    17821 tctggctgcc tcggtcagat ctctggtcgt agccgcaaag gcccggatct gtccctcagc
    17881 cgccgtggcc cggattacat aatctgccat ttttatcttc ctttctctgt ctttcccttt
    17941 tctctcagga tcacatagat ccgttcgctg tcctctttta ccggatcctt tgtaaatgcg
    18001 tcgtaggctg taacaaattc cattccggct gcctcagccg ccgccttcac ctcatccaaa
    18061 gaatagcatc tctgaaaatg agtttcctca aacttccggt acagctcccc ctcccggata
    18121 aaaagagtca gatcgtactc attgatccgc tccttctcat cataataatt ctgccagata
    18181 aagctgctct ctgtgcggtc ctccgcaaag gtattttccc ccataagctc tttatatttg
    18241 tattctgtat tcagatcaaa gataaatacg ccaccgggat ccagataatt attcgccagg
    18301 cggaacacct gcgtcagctc ctccggctcc ataatgtaat tcatggaatc gcagatactc
    18361 accaccgccc ggacggtgcc gtaaagctca aattcccgca tatcctgcaa aagataaagg
    18421 atatccaggc cggccgcagc ccgcttttcc atggcgatct ccagcatctc ctccgaattg
    18481 tcagcgccga tcatatcgta tcctgcctct gccagaagct gcgtcatgct tccggtgccg
    18541 catcctaaat ccagcaccag gccatcctct gcgccgtact cctttaacag ctccttcaga
    18601 taagcggccc attcctcata gggaacattg tcctgaaaca ggtcatagac ctgtgcaaag
    18661 ctcgtatatg cttccatctg cctgcctcca tctttcccct gtaacgccaa ctgcctgtcc
    18721 ggaaataccg ggcaggcagt ctgtgcagct tatttacgcg ttcttaccgc agcacttctt
    18781 atacttcttg ccgcttccgc agggacaggg atcatttggg aagatcttct tttccttgcg
    18841 gatggtgcct gaagccttct gtgccttata cagctccttg cgcttctcct cagacaaaat
    18901 gctgtcccac tgtggaagct catagagcca gtttgccttt gcctctacca tgttgtagta
    18961 cagcttctca ggatcgatct cgatctttac ctctgtatcc tcctccatgg tatcgatcgg
    19021 gttctcataa ccctttaagc tctcattgat gccgtctaaa aagccggtca tgatcagaag
    19081 ctctgtgttg tacttctcag ccagctcctt tacagtacct gtgatcacct ctgtaggtgc
    19141 ggaaaggatc tgctcgtaga tacccttctc aatctggaaa tatccagccc acagttcttc
    19201 tttcttttta tcatccaggc cgtcaccata tgccagattt ctccatgttt ctaataatgc
    19261 catagttaaa ccatcctttt tgtatattta tagaagccta tagtattcta tagtatataa
    19321 caaaacatga gatttttcaa atggttttgt tggagtttgt aatgaaattc cccataaaac
    19381 gggggaaggc ccggggtact ctgctttcgc aggcactgcc gggccgcgtc atctctgact
    19441 gtcctcacta taccatggac tggacaaaaa atcaatggct gtcagggaaa cttaaaaaaa
    19501 cgtaatattt tcgtaacaga tgacgccatt ttactgcatc ttatttacaa aagattcgca
    19561 gtccgtacat tccttcttgg taggattcat ctcgtgggta ccgatcttaa tcgtatcaag
    19621 agtacagtaa tcggtggtat ctgcatgata cgcacaattc ttgatggtac actggataca
    19681 ttcgttcgtt ccgtttactt tcatggtagt tcctccttta tggatgggtg t
//
LOCUS       DBMDUH010000033        19643 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_1683_length_19643_cov_5.446218, whole genome shotgun sequence.
ACCESSION   DBMDUH010000033 DBMDUH010000000
VERSION     DBMDUH010000033.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 19643)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 19643)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19643
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1683_length_19643_cov_5.446218"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="ACZPCG_04575"
     CDS             complement(<1..252)
                     /locus_tag="ACZPCG_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIR1284385.1"
                     /translation="MIMKLSKIAMTGLFAALSYVVFTFLQIKITLPGGDATSIHLGNA
                     VCVLGALILGGFYGGLGGAIGMTIGDLFDPVYVIYAPKTF"
     gene            complement(354..1004)
                     /locus_tag="ACZPCG_04580"
     CDS             complement(354..1004)
                     /locus_tag="ACZPCG_04580"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HIR1284386.1"
                     /translation="MKRCLILTGGRLDPAFAGSFLQTEEFNRVIAVDGGLKGAKELGL
                     VPDVIVGDFDSVDPAVLAFYRKMEHIVWEVHQPEKDDTDTELALKRAMAMGAGEIVIL
                     GATGGRLDHMIGNIQLLYPCLQKGIRASIIDPQNRLYLIDGETVFRRHEVWGTYISFL
                     PLTQEVTGITLKGFKYPLENKDICIGTSLCISNELIEEEGRITFSEGVLMAVESHD"
     gene            complement(1001..1666)
                     /gene="rpe"
                     /locus_tag="ACZPCG_04585"
     CDS             complement(1001..1666)
                     /gene="rpe"
                     /locus_tag="ACZPCG_04585"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HIR1284387.1"
                     /translation="MYYKVAPSVLAADFAELGSQLRAVSEAGAEWIHLDVMDGAFVPS
                     ISFGMPVIKSIRRVTDRVFDVHMMVEEPGRYVEDVKKAGADVITVHAEACKHLDRVIS
                     QIKEAGCRAGVALNPATPVSVLEHILDQVDMVLIMTVNPGFGGQKFIPYTIEKVKAVR
                     RLCDERGLTTDIQVDGGISAANVREVLEAGANIFVAGSAVFGTDPAARTREMVEIFKE
                     YEK"
     gene            complement(1670..2548)
                     /gene="rsgA"
                     /locus_tag="ACZPCG_04590"
     CDS             complement(1670..2548)
                     /gene="rsgA"
                     /locus_tag="ACZPCG_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003523903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HIR1284388.1"
                     /translation="MTGKIIKGIAGFYYVNDGENRVYQCRAKGIFRNRKIKPLVGDNV
                     EFSILDEEAGEGNIDEILPRKNALVRPAAANVDQALVLFALTQPSPNLNLLDRFLVMM
                     AMEEIPVVICFNKADLGDGAMEEEYKKIYEGAGYEVHFISARTDLGMDQVRELLRGRT
                     TVLAGPSGVGKSSLTNRIQPEASMETGGISRKIERGKHTTRHSELFFVEKDTYMMDTP
                     GFSSMYTPEIEASELKEFFPEFAEFEDECRFLGCVHIGERVCGVKEAVKEGKISLSRY
                     ENYRLIYEELKQKRRY"
     gene            complement(2567..4864)
                     /gene="pknB"
                     /locus_tag="ACZPCG_04595"
     CDS             complement(2567..4864)
                     /gene="pknB"
                     /locus_tag="ACZPCG_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HIR1284389.1"
                     /translation="MLNPGTYLQGRYEILEKIGSGGMSVVYKAKCHTLNRLVAIKVLK
                     EEFASDENFVSKFKMEAQAAARLSHPNIVNVYDVVDEENLHYIVMELIEGITLKSYIE
                     KKKLLDSKEAIGIAIQVAQGIAAAHEQHIIHRDIKPQNMIISKDGKVKVADFGIARAV
                     SSQTVNSSAAVGSVHYISPEQARGGYCDERSDIYSFGITLYEMVTGRVPFEGDNTVAV
                     ALAHLEDPVVPPGDYNPQVYPGLEDIILKCTKKKPDRRYGSMEEVIHDLRRVLMDPEC
                     DIYQNEEIEEGGDPYQTRPISKDELSQIRDHHRRKSRETGADEAEKGTEGLETSDEEN
                     GSPDSLSKEDAEEFGDGTGDHRSRYHSSRKREFHKKIPSRKRDEDVSTQFERMIAAIG
                     IIAAILVVIVVVFVFSRLTGLFWPGSGQDSQENQTEAVTQSASMEEIQVIPDEGETIM
                     PNVLDLPRDMAESKLKEYDIVMKVTGEEYSENYSKGYVMRQDVDEGTAVEKWSTVGVI
                     ISKGSERVDVKALNLSGMDKKQAEEILKDKDLIPSAKEEANDTVPKGKVIRVGTEEAK
                     TGDTVELFVSSGPKTVQGQVPKLTDGDAAAADALLKAAGLVSGTVTYEYDPSKPHGQV
                     LSQSELPGTMLEPQSAVDYVVNDSAQASTEAAVAKTGEEEKYYVGSIDATCSLSNYIG
                     PASQTSSVRILIRLKQKDPKDANTYVYTNLISPRLVVGSQDIPVVFSRIKGAYGVDSG
                     IVEVVDVDNDDKVIQSWPVSFFPAG"
     gene            complement(4858..5604)
                     /locus_tag="ACZPCG_04600"
     CDS             complement(4858..5604)
                     /locus_tag="ACZPCG_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HIR1284390.1"
                     /translation="MEAYALTDIGRVRSMNQDYIYASQETIGSLPNLFLVADGMGGHQ
                     AGDFASRYAVEHLVVYLNRCQKGAAVAQLQEGIRQVNAGLYDESLKREELSGMGCTLV
                     AAVIEDGILYAANIGDSRLYRIHGGRIRQVTKDHSYVEEMVARGKMVRGSADYNRRKN
                     IITRAMGIGTHVEADFFEADLESGDYILLCSDGLTNMVADERICAIISEPGTLKEKAE
                     RLIEEANESGGIDNIAVVLVKFEEGGTGLC"
     gene            complement(5606..6667)
                     /gene="rlmN"
                     /locus_tag="ACZPCG_04605"
     CDS             complement(5606..6667)
                     /gene="rlmN"
                     /locus_tag="ACZPCG_04605"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual-specificity RNA methyltransferase RlmN"
                     /protein_id="HIR1284391.1"
                     /translation="MLDLTDRNGKKDIKSMTLEEVTAELGTLGEKPFRAKQLYDWLHV
                     KLVTDFDQMSTLSKSLRERLKEHYSVTALKVVGERISAVDGTRKYLFGLEDGNVIESV
                     WMKYHHGNSVCISSQVGCRMGCRFCASTLDGLERNLRASEMLDQIYRIQTITGERVSN
                     VVIMGSGEPMDNYDNVIRFLRLISHEKGLNISQRNLTVSTCGIVPGILKFAKEGLQVT
                     LALSLHAPNDEVRKTLMPVANSYRLEDILDACHTYFEATGRRLTFEYSLVKGVNDNLK
                     EAAALTKLIKDQHGHVNLIPVNPIKERDYVQSDRRAIEAFRNYLEDHGINATVRREMG
                     RDIGGACGQLRKSYIEGGG"
     gene            complement(6703..8064)
                     /gene="rsmB"
                     /locus_tag="ACZPCG_04610"
     CDS             complement(6703..8064)
                     /gene="rsmB"
                     /locus_tag="ACZPCG_04610"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HIR1284392.1"
                     /translation="MAKQTDRGLENREIILGVLTEVLEKGSFVHLVLNQALYKYQYLD
                     KADRAFITRVTEGTLEYMLQLDGVINRYSKTKTNKMKPLIRNLLRMSVYQLLYMDRVP
                     DSAVCNEAVKLAVKHRFQGLKGFVNGVLRTISREKEHISFENPAMRFSVPDWMYDKWV
                     RDWGEERAEIIAASFLEPRPTWVRCNESTVPVSQILKSLQEEGAGAECMEGFDSMIAL
                     WGYDYLEGLTAFLEGWIQVQDISSSLVGQLAAPKKGDIIIDVCAAPGGKSLHLADQLK
                     RTGLVEARDLTEQKVSLIEDNIARCGLTNIQTKVWDATVFDPSSEEKADIVIADLPCS
                     GMGIIGRKPDIKYHMTEESLKALEELQRQILSVVWRYVKPGGTLIYSTCTINKGENEK
                     NASWIRGNLPFEPVDIRGKLPEGWEAASSASLQEGQIQILPGIGPWKEFDGFFISVFK
                     RRG"
     gene            complement(8077..8775)
                     /locus_tag="ACZPCG_04615"
     CDS             complement(8077..8775)
                     /locus_tag="ACZPCG_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006780421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HIR1284393.1"
                     /translation="MFYMPYFDPTYVLILIGMVLSLMASAKLNATYSTYARVGSRCGL
                     TGAEAAERLLRSQGIYDVAVRRVSGHLTDHYDPRTKTVNLSDAVYGATSIAAIGVAAH
                     ECGHAMQDAGEYVPLRLRAALVPVANFGAQISWPLILIGFFIGGGFLVDIGILLFSAA
                     VLFQLVTLPVEYNASHRAVKLLDSIGILRDQEVGQARKVLGAAALTYVAAAAASILQL
                     LRLLLLFGNRDRDN"
     gene            complement(8821..9753)
                     /gene="fmt"
                     /locus_tag="ACZPCG_04620"
     CDS             complement(8821..9753)
                     /gene="fmt"
                     /locus_tag="ACZPCG_04620"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HIR1284394.1"
                     /translation="MRIVFMGTPDFSVPALNALVDAGHEVAAVVTQPDRPKGRGKDVQ
                     MTPVKLQALEYGIPVYQPEKVKDPAFVEILKELHPEAIVVIAFGQILSKAILDIPPYG
                     CVNIHASLLPKYRGAAPIQWAVIDGEKETGVTTMMMDVGLDTGDMLEKTMIPLDPKET
                     GGSLFEKLSQAGGKLILSTLDKLEKGTAVRTPQKDEDSTYAKMLTKSLGRIDWSMEAE
                     AIERLIRGLNPWPSAYTSLHGKTLKLWDGDVLKETTDARPGEVVLCDPRRLVVAAGEG
                     LLSIRQLQLEGKKRMDVETFLRGYSVKEGEILGE"
     gene            complement(9755..10240)
                     /gene="def"
                     /locus_tag="ACZPCG_04625"
     CDS             complement(9755..10240)
                     /gene="def"
                     /locus_tag="ACZPCG_04625"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR1284395.1"
                     /translation="MAVRQIRVIGDEVLNKKCKPVKEMTPRTAELIEDMFETMYEANG
                     VGLAAPQVGVLKKIVVIDVEDGNQYVLINPEIVEQQGAQTGYEGCLSVPGKTGIVTRP
                     DYVKVRALNENMEEYELEGQDLLARAICHECAHLDGQLYVELVEGELLDTEAEEEEEE
                     M"
     gene            complement(10312..12588)
                     /gene="priA"
                     /locus_tag="ACZPCG_04630"
     CDS             complement(10312..12588)
                     /gene="priA"
                     /locus_tag="ACZPCG_04630"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication restart helicase PriA"
                     /protein_id="HIR1284396.1"
                     /translation="MTGLYANVIIDISHESVDKTFQYRVPEKLRGTLKVGQQVYIPFG
                     AGSRRRKGYVVELTDEPEYDVAKLKEIEGIVEHSVTAQSQLIWLAWWMKERYGSTMNQ
                     ALKTVLPVKQKIKEAPKRQIRLIGEKEAFREAVDLAERRGYKARLRLFCLLKEKNPVP
                     YDQAMNRLNLTPAVLKPLEEAGLIRVEIVEKYRNPLLEMKRLLTGETGRASAMDFSES
                     SPIPVLNEEQQAAAYRICREYDRGIRATYLLHGITGSGKTEVYMELIRHVVEKKKQVI
                     LLIPEISLTWQTVMRFYKRFGNRVSLLHSRMSPGERYDQYERARTGDIDIMIGPRSAL
                     FAPFERLGLIIIDEEHENAYQSELSPRYDARETAKERARINQASLILGSATPSLESYT
                     KAVLGEYILLKLTRRAKASAALPQAEIVDLREELKAGNRSIFSRRLKELMEDRLKKGE
                     QTMLFINRRGYANFVSCRSCGEAIRCPHCDVTLTLHKDGRLMCHYCGHTVMQPKLCPS
                     CGSAYIAPFGTGTQKIEEMAVKMFPQARILRMDLDTTSKKGGHQEILSAFARGEADIL
                     IGTQMIVKGHDFPKVTLVGALAADLSLYAPDYRCGEETFDLLTQAAGRAGRSHIPGNM
                     VIQTYQPDHYSIQTAASQDYESFYSQEMNFRRLLGYPPAVGLLTLTLSDGCEERLEKA
                     AEVLEKMTARFPEHFPGKKLACIGPVNAPVYKVNDIYRKILYIKNENYDILIQIRKMA
                     EACLDGMEGISLQSDLAL"
     gene            complement(12585..13913)
                     /locus_tag="ACZPCG_04635"
     CDS             complement(12585..13913)
                     /locus_tag="ACZPCG_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1284397.1"
                     /translation="MQKDLTEGSITGTMLRFALPMILGNLLQQCYNVADTLIVGRFLG
                     ADALAAVGSAYTLMTFLTSILLGLCMGSGAVFSLRFGEKNSARLKISIYVSFVMIAAV
                     ALAVNLAVVVFTDPIMSLLCVPEEIYGMMREYLRVIFSGILAVFLYNFYASLLRAVGN
                     SLVPLIFLGISAVLNIILDLVFVLVLHRGVAGAAEATVISQFVSGIGIGIYTLVKMPE
                     FRVEREHMKIERQVLGEISQFSFLTCIQQSVMNFGILMVQGLVNSFGTVVMAAFAAAV
                     KIDSFAYMPVQDFGNAFSTFIAQNYGAGKKDRIRAGIRSAVTVSVLFCLFISFVVCVF
                     AKPLLLIFVQPHETEILKVGMSYLRIEGAFYCGIGCLFLLYGLYRAVRRPGMSVVLTV
                     ISLGTRVILAYVLSAVPSIGVTGIWAAVPIGWFLADLAGFVYYKRKLDEI"
     gene            complement(14055..15389)
                     /locus_tag="ACZPCG_04640"
     CDS             complement(14055..15389)
                     /locus_tag="ACZPCG_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HIR1284398.1"
                     /translation="MELTKETCRQTAEELLHFLEKSPSSFHAIANITNMLEAAGFEEI
                     REEENWKLLPGGSYYVTRNQSSVIAFKVPGADFTGFQIMASHSDSPSFKIKENPEMEA
                     EGHFIKLNVEKYGGMIMSPWFDRPLSIAGRLVVEEDGRLVSKLVNIDRDLVMIPNLAI
                     HMNRQVNDGYKYNAQKDTLPIFGSLEAKGSFMTLMAEAAGVKAEDILGHDLFLYSRDK
                     GTIWGADEAYLSCGRLDDLQCAFSSIKGLLAGGHPSCVSVAAVFDNEEVGSGTKQGAD
                     STFLEDTLRRINRSLGRSEDQYLMALASSFMVSADNAHAVHPNYGEFADPTNRPAMNE
                     GIVIKYNANQKYTTDAVSAAIFRAICKKAGVPCQTFANRSDMAGGSTLGNISNAHVAL
                     NTVDIGLPQLAMHSPYETAGVKDTCYLIQAAKTFFNTCLKAEGKGCYRVDQE"
     gene            complement(15415..16854)
                     /locus_tag="ACZPCG_04645"
     CDS             complement(15415..16854)
                     /locus_tag="ACZPCG_04645"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002603929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HIR1284399.1"
                     /translation="MREITAKQAARAAGGRVIFGDPEKKICHISLDSRKMEGRDLFVP
                     IVGERVDAHRFICQAISAGAETVFTSCHHSEKEVREAIESQCREHPEWSGQALLSTWI
                     CVEDTKAALQALGAWCRSQAQIPLVGITGSVGKTTTREMVACALSAGYRVYKTPGNSN
                     SQVGVPITVFNIPKEAQIGVIELGMSEPGEMTKIAQVAQTDCAVMTNIGVAHIEQLGS
                     KENILKEKLHIQDGMAEDGVLFLNGDDPLLRAVMPNQGRSRILYGLWEGCDCRGENLH
                     LENGFPVFTAVCKEERVQVHLKVMGNHMVSNALAALAVASRYGIPLQKAAAALEQFDG
                     FKGRQQIIKEKGVTIIDDSYNASPVSMKAGIEVLSSMAGVKRRIAVLADMKELGPDTI
                     TYHREVGAFLKHQPIDQLVLMGELAWEIETGLKMDGGKISCVKTSTLEETETWLAEAV
                     REGDCLLFKGSNSMGLSKAVSFMREKLTN"
     gene            complement(16851..18371)
                     /locus_tag="ACZPCG_04650"
     CDS             complement(16851..18371)
                     /locus_tag="ACZPCG_04650"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HIR1284400.1"
                     /translation="MILQDWLKELDYTLIQGNPDTQVEEVIYDSRKAGPGTVFVAMTG
                     TRIDAHCFIPDVLKAGTRILVTERPIDMELEACELDEEEKKKITILRVKDSRRALALL
                     SAARFGYPAKKLTLIGVTGTKGKTTTTHMIKTVLEAAGKKAGLIGTTGVVIGEHVTPT
                     MNTTPESYELHKAFAQMAEAGCDYAVMEVSSQGVKMHRVDGILFDYGIFTNISPDHIG
                     PDEHKDFEEYLYYKTRLLDSCRIGLLNGADPHAEAIKKEVSCKLYTYNAGKESKVPDD
                     FMAENIRYLAEPDFVGTQFHVSGRCEMEIRLGIPGLFNVENAMAAVSLCSLLGLDPQR
                     VSHALEHIRVNGRMEIVHTSSHCTVLVDYAHNAVSMESLLKTLRDYSPKRLVCVFGCG
                     GNRSKDRRYSMGEIGGRLADLCILTADNSRYEKVEDILADIRGSIEKTGGAFMEIPDR
                     REAIRYSVFHAEPGDMIAVIGKGHEDYQEINGVRHHFLDREVIEEAVRELREKEQG"
     gene            complement(18396..>19643)
                     /locus_tag="ACZPCG_04655"
     CDS             complement(18396..>19643)
                     /locus_tag="ACZPCG_04655"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007717859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HIR1284401.1"
                     /translation="ALLDNHIQQGNSLGIDPRQILWKRCEDMNDRALRNIVIGLGAKA
                     DGMVREDHFVITVASEIMAILCLADDMKDLKERLSRIIVAYNFAGEPVTAGDLHATGA
                     MAALLKDALKPNLIQTLEHTGALVHGGPFANIAHGCNSVRATKTALKLCDIVVTEAGF
                     GADLGAEKFMDIKCRKAGLKPDAVVLVATVRALKYNGGVPKDQLSVENLEALKKGIVN
                     LEKHIENIQKFGVPVIVTLNSFISDTEAEYAYIKHFCEERGCEFALAEVWAKGGEGGL
                     ALAEKVLNTLENRPTDFHVLYENEASLKDKIGTIASQIYGADGVTYTPAALKALKRME
                     EMGFGNLPVCMAKTQYSLSDDQTKLGRPAGFQINVRDAYVCAGAGFVVVLTGSIMTMP
                     GLPKKPAAEGIDVNEEGKITGLF"
BASE COUNT         4627 a         5577 c         4236 g         5203 t
ORIGIN      
        1 aaaggtctta ggggcatata ttacatagac aggatcgaaa agatccccaa tggtcatgcc
       61 gatggctccg cccagaccgc cgtaaaagcc gcccaggatc aaggcaccca gcacacagac
      121 cgcatttccc agatgaatgg aggtggcatc tccgccggga agggtgatct tgatctgcaa
      181 aaaggtaaat actacatagg aaagggctgc aaaaagtccg gtcatggcaa tctttgataa
      241 tttcataatc atccgctcct cacttgcatt ttatgatgca tatggtacca ctaaagtgga
      301 tggttacaaa tatccagttt atcacttttt aaccagtcca gtttctccga tcatcaatca
      361 tgagattcca ccgccatcag aacaccctct gaaaaggtaa tccgtccctc ttcttctatg
      421 agttcattac tgatgcagag actggtaccg atacagatat ctttattttc cagcggatat
      481 ttaaagccct ttaaggtaat tcctgttacc tcctgtgtaa gaggcaggaa ggaaatatac
      541 gttccccaga cttcatgcct gcgaaataca gtttctccgt caatcagata cagacggttc
      601 tgaggatcta tgatgctggc acggattccc ttttgaaggc agggatataa aagctgtata
      661 ttaccgatca tatgatccag acgtcctccg gtggctccaa ggataacgat ttctcctgct
      721 cccatggcca tggcccgctt taaggccagc tctgtatccg tatcgtcctt ttccggctgg
      781 tggacctccc agacaatatg ctccatcttc cggtaaaatg ccagaaccgc cggatcaacg
      841 gagtcaaaat cacctacgat cacatccggc acaaggccca gctcctttgc acctttcagt
      901 ccgccgtcta ccgcgatcac tctgttaaat tcctctgttt gtaaaaagga accggcgaag
      961 gccggatcaa gccgtccgcc ggtaaggatc agacatcgtt tcatttctcg tattccttaa
     1021 aaatttccac catctctctt gtgcgggctg ccgggtctgt tccgaacacg gcgcttcctg
     1081 ctacaaagat attggctcct gcctctaaaa cctctcttac attggctgcg ctgatgcctc
     1141 cgtctacctg gatgtctgtc gtaaggcccc gctcgtcaca aagccttctc actgctttta
     1201 ccttttcgat ggtatagggg atgaacttct gtccgccaaa gcccggatta acggtcatga
     1261 tcaaaaccat gtccacctga tccagaatat gttccagcac agaaacagga gtcgccggat
     1321 ttaaagccac tccggcccga cagccggctt ccttgatctg gctgatcacc cggtctaaat
     1381 gcttacaggc ctcggcatgg acggtgatca catccgctcc tgccttcttt acatcctcca
     1441 catagcggcc cggctcctcc accatcatat gaacgtcaaa cacacgatcc gttacccggc
     1501 ggatgctttt gatcactggc atgccaaaag aaatgctcgg cacaaaggcg ccatccatca
     1561 catccagatg gatccactct gctccggcct ctgaaacagc ccgaagctga cttccaagct
     1621 ctgcaaaatc tgccgccaga actgacggcg ctaccttata atacatgatt taatatctcc
     1681 ttttctgctt aagttcttca tagatcagac ggtaattctc atagcggctg agactgattt
     1741 tcccctcttt taccgcttcc tttaccccgc agaccctttc tcctatgtgg acacagccca
     1801 aaaagcggca ttcatcctca aactctgcaa actccggaaa aaactccttc agctcagaag
     1861 cctctatttc cggcgtatac atggagctga agcccggtgt gtccatcata taagtatctt
     1921 tttccacaaa aaacagttct gaatgacggg tggtgtgctt tccgcgctca atcttccggc
     1981 tgatgcctcc tgtttccatg gatgcctccg gctggatccg gtttgtaagg gaggatttcc
     2041 ctactccgga agggccggcc agcaccgtcg tacgccctct taaaagctca cggacctgat
     2101 ccatccccag atcagtcctg gcactgataa agtgaacttc ataaccagcg ccctcatata
     2161 tctttttgta ctcctcctcc atggcaccat ctcccagatc cgccttatta aagcagatca
     2221 ctaccggaat ctcctccata gccatcatca ccagaaagcg gtccagaaga tttaaattgg
     2281 gggatggctg agtcagggca aagagtacca gcgcctgatc cacattggct gccgccgggc
     2341 gcaccagggc attttttctc ggaaggatct cgtcgatatt cccctctcct gcctcttcat
     2401 ccaggatgga aaattccaca ttatctccta ccaggggttt aattttcctg tttctgaaaa
     2461 taccttttgc cctgcactgg tacacccggt tttcgccgtc atttacataa taaaagcctg
     2521 caattccctt gattatcttt cctgtcatat tgtttcctgt tcttttttat cctgccggga
     2581 aaaaggatac cggccaggat tggatcacct tatcgtcatt atccacatct acaacctcca
     2641 caatcccgct gtcaacgccg tatgcgccct tgatccgcga gaatacaaca ggaatatcct
     2701 gagagcctac taccagtctg ggactgatca ggtttgtgta tacataggta ttggcatcct
     2761 tgggatcctt ttgttttaaa cggatcaaaa tacgcacgct gcttgtctgg gaagcagggc
     2821 caatataatt gcttaaggag caggtagcgt caatggaacc aacataatat ttctcttcct
     2881 ctcctgtttt tgccacagca gcctctgtgg aagcctgagc actgtcatta actacataat
     2941 ccactgcaga ctggggctcc aacatggttc ccggaagttc actctgagaa agaacctgcc
     3001 catgaggctt ggaaggatcg tactcatagg tcactgtacc gctcacaaga ccggctgcct
     3061 ttaaaagagc atctgctgct gccgcatcgc cgtccgtcag ctttggcacc tgtccctgaa
     3121 cggttttagg gcctgagctt acaaaaagct ccaccgtatc tcccgtcttt gcttcttctg
     3181 ttccaacacg gatcaccttt cccttcggca ccgtatcatt tgcttcttct tttgcagacg
     3241 gaatcagatc cttatctttt aaaatctcct ctgcctgctt tttatccata ccggataaat
     3301 taagcgcctt tacgtctacc cgttcgcttc ccttgctgat gataactcct acggtactcc
     3361 atttttctac tgctgtcccc tcgtccacat cctggcgcat tacatagccc ttggaatagt
     3421 tttcggagta ctcttcccct gtaaccttca ttacaatgtc atattcctta agcttgcttt
     3481 ccgccatatc tctgggcaga tctaaaacat ttggcattat agtctctccc tcatccggga
     3541 tcacctggat ttcctccata gatgcggact gggtgaccgc ctctgtctga ttttcctggc
     3601 tgtcttgccc gcttccgggc cagaacagcc ctgtaagtct tgaaaacaca aaaacaacca
     3661 cgatcacgac cagtatggca gctataatgc caatcgcagc gatcatccgt tcaaactgtg
     3721 tgcttacatc ctcgtcccgc tttctggagg gaatcttttt atgaaattct cttttgcggg
     3781 aagaatgata cctgctgcga tgatctcccg tgccgtcgcc aaactcctcc gcatcctctt
     3841 tagacaggct gtctggggat ccattttctt catcagacgt ttcaagaccc tctgtccctt
     3901 tttccgcctc atctgcacca gtctccctgc ttttacggcg gtgatgatcc cggatctggc
     3961 ttaactcgtc tttagaaata gggcgtgtct gatagggatc ccccccttcc tctatttcct
     4021 cattttggta aatatcacat tccggatcca taagcacccg gcgcagatcg tggattacct
     4081 cctccatgga gccatagcgc ctgtccggct tctttttggt acattttaaa ataatatcct
     4141 ccagccccgg atagacctga ggattataat ctcctggcgg gaccaccgga tcctctagat
     4201 gggccagagc cacagcaacc gtattgtccc cctcaaaggg aacccggcct gtaaccattt
     4261 cataaagcgt aattccaaag gaatagatat cgcttcgctc atcacagtat ccgcctctgg
     4321 cctgttccgg tgaaatatag tgcaccgagc ccacagcagc cgatgaatta accgtctggc
     4381 tggaaacggc tctggcaatg ccaaagtctg ctacctttac cttgccgtct tttgaaatga
     4441 tcatgttctg aggcttgata tcccggtgga tgatatgctg ctcatgagct gcagcaatcc
     4501 cctgtgccac ctggatggca ataccgatcg cctctttgct gtctaaaagt ttcttttttt
     4561 caatgtagct tttgagggta atcccctcaa taagctccat cacgatataa tgaagatttt
     4621 cctcatctac tacatcatat acatttacaa tattgggatg ggaaagccgt gctgctgcct
     4681 gagcttccat cttaaacttg cttacaaaat tctcatctga ggcaaattct tccttcagta
     4741 ccttaatcgc taccagacgg tttaaggtat ggcattttgc cttatagaca acagacattc
     4801 cacctgagcc gattttttcc agaatttcat accgcccctg aaggtaggtt ccaggattta
     4861 acataggccg gttccccctt cctcgaattt tacaagaaca accgcaatat tatcgattcc
     4921 tccactctca ttggcttcct cgatcagacg ctctgccttt tccttaagag tgccgggttc
     4981 ggatataatg gcgcagatcc gttcatcggc caccatattg gtaaggccgt cggaacagag
     5041 aagtatatag tcgcccgact ctaaatccgc ctcaaaaaag tctgcctcta catgggttcc
     5101 gattcccatt gcccgggtaa tgatattttt cctgcggtta tagtccgcac ttccccggac
     5161 cattttccct ctggctacca tttcctccac ataggaatga tcctttgtaa cctggcgaat
     5221 ccggcctcca tggatccggt aaagacggct gtctccgata ttagccgcat agaggatccc
     5281 atcctctatc accgctgcta caagcgtgca tcccataccg ctcagttcct ccctcttaag
     5341 ggattcatcg taaaggcctg catttacctg gcggatcccc tcctgcagct gagccactgc
     5401 cgctcccttt tggcagcggt ttaagtacac caccagatgt tccactgcat accgggaggc
     5461 aaaatcaccg gcctgatgac cgcccatgcc gtcagctacc agaaaaaggt ttggaaggga
     5521 accaatggtt tcctgagagg cataaatata atcctggttc atggagcgga ctctccctat
     5581 atctgtcagt gcatatgctt ccatgtcatc cccctccttc tatataactc tttcttagct
     5641 gcccgcaggc tccgccgata tcccggccca tctcccggcg gaccgtggca ttgatgccgt
     5701 ggtcttccag atagttccga aacgcctcaa tggccctgcg gtcggactga acgtaatccc
     5761 gttccttgat gggattaacc gggatcagat ttacatggcc atgctggtcc tttatcagtt
     5821 tcgtaagggc tgcggcctcc tttaaattat catttacccc ttttaccagg ctgtattcaa
     5881 aggtcagccg tctgccggtt gcttcaaaat atgtatggca ggcatctaaa atatcttcca
     5941 gacggtagct gtttgccacc ggcatcaagg tttttcgcac ctcgtcattg ggggcgtgca
     6001 gagacaaggc taatgtgacc tgaagcccct ctttagcaaa ttttaagatt cccggcacaa
     6061 tgccgcaggt ggataccgtc agatttctct ggctgatgtt aagccccttc tcatgggaga
     6121 tcaggcgcag gaaacggatc acgttatcat agttgtccat gggttctccg gaacccatga
     6181 tcaccacatt ggatacccgc tctcctgtaa tggtctggat ccgataaatc tgatccagca
     6241 tttctgaggc ccgcaggttc cgctccagac cgtccagggt ggaggcgcag aagcggcagc
     6301 ccatacggca gcccacctgg gaggaaatgc atacggaatt gccgtgatgg tacttcatcc
     6361 acacactctc gatcacattc ccatcttcca gtccaaagag gtactttctg gtaccgtcca
     6421 cagccgaaat ccgttctcct acaaccttta atgccgtaac gctgtaatgc tctttaagcc
     6481 tttcccgaag gctctttgag agagtactca tctgatcaaa gtctgtcacc agctttacat
     6541 ggagccagtc gtagagctgc tttgcccgaa agggcttttc tcccaatgtg ccaagctctg
     6601 cggttacttc ctccagggtc atggatttga tatctttttt accattgcgg tctgttaaat
     6661 ctaacataaa aaactcctgt actttaaaat aaaccggtct ttttatcccc ggcgcttaaa
     6721 aacgctgata aaaaagccgt caaattcctt ccatgggcca atccccggca ggatctggat
     6781 ctgtccctcc tgtaaagaag cagaagaggc tgcttcccat ccctccggga gctttcctct
     6841 tatatccaca ggctcaaagg gaagattccc gcgaatccag gaagcatttt tctcattttc
     6901 tcccttattg atggtgcagg tactgtaaat cagtgttccg ccgggcttta cataccgcca
     6961 tacaacggat aagatctggc gctgcaattc ttccaaagct ttcagacttt cttctgtcat
     7021 gtggtacttg atatccggct ttctcccaat aattcccatg ccggaacagg gaagatctgc
     7081 gatcacgata tctgcctttt cctcactgga cgggtcaaac acagtggcgt cccatacctt
     7141 tgtctgaata tttgtcagac cgcagcgggc aatattgtcc tcaataaggg aaaccttctg
     7201 ttctgttaaa tccctggctt ctaccaggcc ggtccgtttt agctgatctg ccagatgaag
     7261 gctctttcct ccaggagcag cgcatacatc aatgatgata tcaccctttt ttggagcagc
     7321 cagctgtcct accaggctgg agctgatatc ctgaacctgg atccagccct ccaagaatgc
     7381 agtcagccct tccagatagt catagcccca cagggcgatc atggaatcaa agccttccat
     7441 acactctgca ccggctccct cttcctgaag agattttaat atctgagata ccggtacggt
     7501 actttcattg catcgaaccc aggtaggacg gggctctaaa aaggatgcgg caatgatctc
     7561 ggccctctct tctccccagt ctctgaccca tttatcatac atccagtctg gaacactgaa
     7621 acgcatggct ggattctcaa aggaaatgtg ttccttttct ctggaaatgg tacgcagcac
     7681 tccgtttaca aagcccttta aaccctgaaa ccgatgcttc actgccagct ttaccgcttc
     7741 attgcacaca gcgctgtcag gcacccggtc catatagaga agctgataga cgctcattct
     7801 taaaaggttc cggatcagtg gcttcatctt gttcgtcttt gtctttgaat accggttgat
     7861 caccccgtcc agctgaagca tatactccag tgtcccttct gtgacacggg tgataaaggc
     7921 tctgtctgcc ttatccagat attgatattt ataaagagcc tggtttaaaa caagatggac
     7981 gaagctgccc ttttcgagca cctccgtcaa caccccaagg attatttccc tgttttccag
     8041 acccctgtct gtctgttttg ccataaatcc ctcctgttaa ttatccctgt ctctgtttcc
     8101 gaataaaaga agcagacgaa ggagctggag gatggaagct gctgcagcag ctacataggt
     8161 taaggcagct gcgcctaata cctttcttgc ctgccccact tcctgatccc gaagaatccc
     8221 aatggaatcc agaagcttta ctgcacggtg ggaagcatta tattctaccg gcagggtcac
     8281 aagctggaaa agcacggcgg ctgaaaaaag caggatccct atatcaacca gaaagcctcc
     8341 gcctataaag aagccgatga ggatcagggg ccaggagatc tgggcgccga agttggctac
     8401 cggcaccaat gcagctctta accgcagggg aacgtactct cctgcatcct gcatggcatg
     8461 gccgcactca tgggccgcca caccgatggc tgcaatggag gtggctccgt ataccgcatc
     8521 cgacaggttt actgttttgg tgcgggggtc atagtgatct gtaaggtgac cggatacacg
     8581 gcgcactgcc acatcataga tcccctggga acggagcagc cgctctgccg cctctgctcc
     8641 cgtaagcccg cagcggcttc ccacacgggc ataggtgctg taggtagcgt tcagttttgc
     8701 agaagccatt aaagacagca ccattccgat gaggatcaga acgtaggtag ggtcaaaata
     8761 gggcatataa aacatataga ttcactctct ttctaacgta acagcatgat tgcgggaaac
     8821 ttactctccc agtatttctc cctcttttac ggaatagcct ctgaggaagg tttccacatc
     8881 catccgcttc tttccttcca gctgaagctg gcggatggat aacagccctt ctcctgcagc
     8941 cactaccaga cggcggggat cacaaaggac tacctctccc ggtctggcat ccgtcgtttc
     9001 cttcaaaaca tccccatccc agagctttaa ggtctttcca tgaagggagg tataggcgct
     9061 gggccaggga tttaaaccgc ggatcaggcg ttcaatggcc tctgcctcca tagaccagtc
     9121 aatgcgtccc agagactttg taagcatctt tgcataggta gaatcctcgt ccttctgagg
     9181 cgtccttact gcggtgccct tctcaagctt atcaagggtg gaaagaatca gctttccccc
     9241 tgcctggctg agtttttcaa acaggcttcc tccggtttcc ttcggatcca gagggatcat
     9301 cgtcttttcc agcatgtctc ccgtatccag tcccacatcc atcatcatgg tggtaacgcc
     9361 ggtttccttc tctccgtcga tcaccgccca ctggatggga gctgctcccc ggtactttgg
     9421 aagcagggag gcatggatat tgacgcagcc atagggcgga atatccagaa tggcctttga
     9481 gaggatctgt ccgaaggcga ttactacgat cgcctccgga tgaagctcct ttaaaatctc
     9541 cacaaaagcc ggatccttta ccttttccgg ctgatagacc ggaatgccgt actctaacgc
     9601 ctgaagcttc accggcgtca tctgtacatc ttttcctctg ccctttggac gatccggctg
     9661 ggtcactacc gcagctacct cgtggccggc atccaccagg gcatttaatg ccggaacgga
     9721 aaaatccggc gtccccataa atacgatacg catcttacat ctcctcttcc tcttcttcag
     9781 cctctgtatc cagcagctcg ccttcgacca gctctacata gagctggcca tccaggtgtg
     9841 cgcactcatg acagatggct ctggctaaaa gatcctgtcc ctctaactca tactcctcca
     9901 tgttttcatt cagcgctctt acctttacat aatccggacg ggttacgatg ccggtcttgc
     9961 cgggaacgct taagcagccc tcgtatccgg tctgggcgcc ctgctgctcc acaatttccg
    10021 ggttgatcag cacatactga ttgccgtcct ctacgtcaat taccacgatt ttctttaaaa
    10081 cgcccacctg gggagctgca aggcctacgc cgtttgcctc atacatggtc tcaaacatat
    10141 cttcgatcag ctctgccgta cgaggagtca tctcctttac cggcttgcat tttttattta
    10201 aaacttcatc tccaattaca cgaatctgac ggactgccat tttattatct ctcctttttc
    10261 atattcaaac cataaacggc ggatatacaa accccttttc tactgtttgg cctataaagc
    10321 cagatcggac tggagggaaa tcccttccat gccatccaag caggcctccg ccatttttct
    10381 gatctgtatc agtatatcat agttttcatt cttaatatat aaaatttttc tgtaaatatc
    10441 attaactttg tacaccgggg cattcacagg gccaatgcag gcaagttttt ttcccggaaa
    10501 atgctccgga aaacgggctg tcatcttctc cagtacctct gcggcctttt caaggcgctc
    10561 ctcacagccg tcggacaaag tgagtgttaa cagccccact gcgggaggat atcccagaag
    10621 ccgcctgaaa ttcatctcct ggctgtaaaa gctttcatag tcctggctgg ctgccgtctg
    10681 tatgctgtaa tgatccggct gataggtctg gatcaccata ttccccggaa tatggctcct
    10741 tccggccctt cctgctgcct gggttaaaag atcaaaggtt tcctccccgc agcggtaatc
    10801 cggagcatag agggagagat cggccgccag agccccgacc agagtgacct ttggaaaatc
    10861 gtgtcccttt acgatcatct gggtaccgat caggatatcg gcctctcctc ttgcaaaggc
    10921 agaaaggatt tcctgatggc ctcccttttt tgaggtggta tccagatcca tcctgagaat
    10981 ccttgcctga ggaaacattt ttactgccat ttcttcaatc ttctgggttc ccgttccaaa
    11041 gggcgcaata taggcggaac cgcaggaggg gcagagcttt ggctgcatca ccgtatgacc
    11101 gcagtaatgg cacatcagac ggccgtcttt atgaagcgtc agggtcacat cgcagtgggg
    11161 acagcggata gcctctccgc aggaacggca ggatacaaaa tttgcatagc cgcggcggtt
    11221 gataaaaagc atggtctgct ctcctttttt cagcctgtcc tccatcagtt ccttcagcct
    11281 gcggctgaaa atagaacggt ttcccgcctt taattcctct ctcagatcta cgatctccgc
    11341 ctgaggcagt gccgcgctgg cctttgcccg tcttgtgagc ttaagaagga tatattctcc
    11401 cagaaccgcc tttgtgtagg attccagaga aggcgtggca gagcccagga tcaggctggc
    11461 ctgattgatc ctggccctct cctttgccgt ctccctggca tcatagcggg gggacagctc
    11521 gctctgatag gcattttcat gttcctcatc aataatgatc agccccagcc gttcaaaggg
    11581 ggcaaacagg gcagaacggg gcccgatcat aatatctata tccccggttc ttgcccgttc
    11641 atactggtca tagcgctccc ctggggacat tctggagtga agcagggaaa cccggttccc
    11701 gaaacgttta taaaatcgca tgaccgtctg ccaggtaagg gaaatttccg ggatcaaaag
    11761 gattacctgc ttttttttct ccaccacatg gcggatcagc tccatataaa cctctgtttt
    11821 tccgctgcct gtgatcccat ggagcagata tgtggcgcgg atccctctgt catactcccg
    11881 gcagatgcgg tatgcggcag cctgctgttc ctcatttaag acgggaatcg gactgctttc
    11941 ggaaaaatcc atggcagaag cccttcccgt ttcccctgtg agaagccgct tcatctcaag
    12001 aaggggattt ctgtattttt ctacgatttc tacccggatc agccccgctt cctccaaagg
    12061 cttaaggacc gccggggtca ggttcagacg gttcatggcc tgatcgtagg gaacgggatt
    12121 tttctctttt aacaggcaaa acaggcgcag cctggcctta tagcctctgc gctctgccag
    12181 atccaccgcc tcccggaagg cctctttttc gccaatgagc cggatctgcc gtttgggagc
    12241 ttcctttatc ttctgcttca caggaaggac tgtctttaat gcctggttca tggtagagcc
    12301 gtagcgctcc ttcatccacc aggccagcca gatcagctgt gactgggctg taacgctgtg
    12361 ctctacaatc ccttctattt cctttaattt tgccacatcg tattccggct catccgtaag
    12421 ctccaccaca tatccctttc tcctgcggct tcccgctcca aagggaatgt aaacctgctg
    12481 gcctacctta agagttcctc taagcttctc aggaacccgg tactgaaagg tcttatccac
    12541 gctttcatgg gaaatatcaa taattacatt ggcatataat ccggtcaaat ctcatccaac
    12601 tttcttttat aatagacaaa gcctgccaga tcggctaaaa accagccaat gggcacagcc
    12661 gcccagatcc cggttacccc aatggacgga acggcggaca ggacataggc taaaatcacc
    12721 cgggtgccca gggaaatcac agtcaggacc acggacattc ccggtcttct cactgcccgg
    12781 taaaggccat agagcaaaaa cagacagccg atcccacagt aaaacgcacc ctcaatccga
    12841 agataactca tccccacctt aaggatctcc gtttcatggg gttggacaaa aatcagaagg
    12901 agcggctttg caaatacgca taccacaaag gagataaaca ggcaaaacag aacggaaacc
    12961 gtcacagcag aacggattcc tgcacggatc ctgtcttttt ttcctgctcc gtaattctga
    13021 gctataaagg tggaaaatgc atttccaaag tcctgaaccg gcatataggc aaaggaatcg
    13081 atctttaccg ctgcggcaaa ggcagccatc accaccgttc cgaagctgtt taccagtccc
    13141 tgtaccataa ggatgccaaa attcatcaca gactgctgga tacaggtaag aaaggaaaac
    13201 tgggaaattt ctcctaaaac ctggcgctct atcttcatat gttcccgctc cacccggaac
    13261 tcaggcattt ttacaagcgt ataaataccg attccaatac cggaaacaaa ctgggaaata
    13321 accgtggcct ctgccgctcc cgctacgccc cggtgaagca ccaggacaaa taccagatcc
    13381 agtatgatat ttaaaacggc tgaaattccc agaaaaatca gcggcaccag agaatttccc
    13441 acagcccgca gaagagaggc ataaaaatta tacagaaaaa ctgccaggat acctgaaaag
    13501 atcactctca ggtattcccg catcatcccg taaatctctt ccggtacgca cagaaggctc
    13561 ataatgggat cggtaaacac caccaccgcc aggttgacgg ccagagccac cgcagcaatc
    13621 attacaaagg atacatagat gcttattttt aaacgggcgc tgtttttctc cccaaagcgc
    13681 agggaaaaga cggccccgct tcccatacac agtcccagaa gaatagaggt aagaaaggtc
    13741 ataagggtat aggccgagcc tacggctgcc agggcatccg cccccagaaa tcggcctacg
    13801 atcagcgtat ctgccacatt gtagcactgc tgtaaaagat ttcccagtat catcggaagg
    13861 gcaaagcgca gcatagtgcc tgtaatgctt ccctctgtaa ggtctttctg catggtttct
    13921 tctaatctcc tctttccgtt tattaatata cccctatccg gctgactgtc tttgcccgga
    13981 cgcactccgg cctctgggct ttatggtgca aagccagagg ccgtgtatat cagggtgttt
    14041 tcccccttat gatcttattc ctgatccacg cggtagcagc cttttccctc tgcttttaaa
    14101 caggtattaa agaacgtctt tgctgcctgg atcagataac aggtatcctt tacgccggct
    14161 gtctcataag gggaatgcat cgccagctgg ggcaggccga tatccactgt attgagagcc
    14221 acatgagcat tggaaatatt tcccagagta gaacctcccg ccatatcgga acggttggca
    14281 aaggtctggc agggaacgcc ggcctttttg cagattgccc ggaaaatagc tgcggatact
    14341 gcatctgtag tatacttctg gttggcgttg tacttgatta caatcccctc gttcatggca
    14401 ggacggttgg tcggatctgc aaattctccg taatttggat gaaccgcatg agcattgtca
    14461 gcagatacca taaagctgga ggccagagcc atcagatatt gatcctcgct gcgtcccaga
    14521 ctgcggttga tccggcgcag ggtatcctct aagaaggtgg aatctgcacc ctgtttagtg
    14581 ccgcttccca cttcctcatt gtcaaatacg gctgcaacgg atacgcagga aggatgtcct
    14641 cctgccagca ggcccttaat ggaagaaaaa gcacactgca gatcatccag acgtccgcag
    14701 gaaaggtaag cctcatcggc tccccagatg gttcccttgt cgcggctgta aaggaacagg
    14761 tcatggccca ggatatcctc tgcctttacg cctgctgctt ctgccatcag ggtcataaag
    14821 cttccctttg cttccagact tccaaaaata ggaagcgtat ccttctgtgc attatacttg
    14881 taaccgtcat tgacctgtcg gttcatatga atagccaaat tagggatcat caccagatca
    14941 cggtctatat tcactaactt tgataccagt cttccatcct cttctacaac caggcggccc
    15001 gcaatggata aaggacggtc aaaccaggga gacatgatca tgccgccgta tttttctacg
    15061 tttaatttga taaaatggcc ctctgcctcc atctccggat tctccttaat cttaaaagaa
    15121 ggggaatcgc tgtggcttgc catgatctga aagcctgtaa aatctgctcc cggcacctta
    15181 aatgcaatga cagaagactg attgcgggtc acatagtaag agccgcccgg taaaagcttc
    15241 cagttttcct cctcacggat ttcctcaaag cctgctgctt ccagcatatt cgttatattg
    15301 gcaatagcgt gaaagctgct ggggcttttc tccagaaagt gcagcagctc ctctgccgtc
    15361 tgacggcagg tttctttcgt taattccatg tgttttttcc tccagataat cattttaatt
    15421 cgtgagtttt tctctcataa agctcacggc tttggaaagc cccatgctgt ttgaaccctt
    15481 aaacagcagg cagtcgcctt ctcttactgc ttctgccagc catgtttcag tttcctccaa
    15541 agtggaggtt tttacgcatg agatttttcc tccatccatt ttaagacccg tttcaatctc
    15601 ccatgccagt tctcccataa gcaccagctg gtcaattggc tgatgtttta aaaaggcacc
    15661 tacctccctg tggtacgtta tggtatccgg cccaagctcc ttcatatccg ccagaaccgc
    15721 aatgcggcgc ttcactcctg ccatggagga aagcacctca atgcctgcct tcatggaaac
    15781 ggggcttgca ttgtagctgt catctatgat ggtgacgcct ttttccttaa tgatctgctg
    15841 gcgtccctta aaaccatcaa actgttctaa ggcagcagca gccttctgta acggtattcc
    15901 ataccgggaa gccactgcca gggctgccag ggcattgctt accatgtgat ttcccattac
    15961 cttaaggtgt acctgtaccc gctcttcttt gcagactgcg gtaaagacag gaaagccatt
    16021 ttccagatga aggttctctc cccggcagtc gcagccttcc cacaggccat agaggatccg
    16081 gctgcgtccc tggttcggca ttaccgctcg caggagagga tcgtcgccat tgaggaaaag
    16141 aacaccatcc tctgccattc catcctggat atggagcttc tcttttaaaa tattttcctt
    16201 agagccaagc tgttcgatat gagccactcc aatattggtc atcactgcac agtccgtctg
    16261 tgctacctga gcaatctttg tcatctctcc gggctcgctc ataccaagtt caataacgcc
    16321 gatctgcgct tcctttggta tattaaaaac agtgatgggg acacccacct ggctgttgct
    16381 gtttcccggc gtcttataaa ccctataacc ggcgctcaag gcacaggcta ccatctcacg
    16441 ggtggtcgtc tttcccacac tgcccgtaat ccctaccaga ggaatctgcg cctggctgcg
    16501 gcaccaggct cccagcgcct gaagagccgc ttttgtatcc tctacacaga tccaggtgct
    16561 taaaagagcc tgtccgctcc attcaggatg ctccctgcac tggctttcaa tggcctctct
    16621 tacctctttt tcactgtgat gacaactggt gaacaccgtt tctgccccgg cgcttattgc
    16681 ctgacagata aaccggtgtg cgtctacccg ttctccgacg ataggaacaa acagatccct
    16741 tccctccatt tttctggaat ccaggctgat gtggcagatc tttttttccg gatcaccgaa
    16801 aatcaccctg cctccggcgg ctctggctgc ctgtttcgct gttatctctc tcatccctgt
    16861 tccttctctc tcagttccct tactgcttct tcaatcactt cccgatccag aaaatgatgg
    16921 cggacaccat tgatctcctg ataatcctca tggccttttc caatcaccgc gatcatatct
    16981 cccggctctg catggaaaac actgtatcgg atggcttccc tgcggtccgg tatctccata
    17041 aaggctccgc ctgttttttc aatacttccc cggatatccg ccagaatatc ctccactttt
    17101 tcatatctgg aattatccgc tgtgaggatg cacaaatctg ccagccttcc gccgatttct
    17161 cccatagaat aacggcggtc cttagaacgg tttccaccgc agccaaatac acacaccaga
    17221 cgtttggggc tgtaatcccg caatgtctta agcaggcttt ccatgctcac tgcattgtgg
    17281 gcatagtcaa ccagcacggt acagtgggag gatgtatgta cgatttccat gcgtccgttt
    17341 acccggatat gctccagtgc atggctgacc ctttgaggat ccagccccaa taagctgcac
    17401 aggctgactg cagccatggc attttccaca ttaaaaagtc ccggtatgcc aaggcggatt
    17461 tccatctcac atctgccgga aacatgaaac tgcgttccca caaagtccgg ctctgccaga
    17521 taccggatat tctctgccat aaagtcatcc ggcactttgc tttcctttcc tgcattgtag
    17581 gtataaagct tacaagaaac ttccttcttt atggcctctg catgagggtc tgccccgttt
    17641 aaaaggccaa tgcggcagct gtcaaggaga cgtgttttat aatacagata ctcctcaaaa
    17701 tccttatgct catccggccc gatatggtct ggggaaatat tagtaaaaat accgtagtca
    17761 aacaggatac cgtctacccg gtgcattttt accccctggg aagaaacttc cataaccgca
    17821 tagtcacagc ctgcctctgc catctgagca aaggctttgt gaagttcata ggattccggg
    17881 gttgtgttca tggtaggagt cacatgttct ccaatgacaa cacctgtggt accgatcaga
    17941 ccggccttct ttcctgccgc ttccagcaca gtctttatca tatgggtcgt ggtagtcttt
    18001 cccttggtgc ctgtaacgcc gatcagggta agcttctttg caggatatcc aaaacgggcc
    18061 gcagacagca gggccagagc cctgcgggaa tcctttaccc gcaggatcgt aatctttttc
    18121 ttctcctctt catcaagctc acaggcttca agctccatat caatgggccg ttcggtcaca
    18181 aggatccttg tgcctgcctt taacacatca gggataaagc agtgggcatc gatccgggtg
    18241 cctgtcatgg ccacaaatac agtgcccggt cccgcttttc tggagtcata aataacctct
    18301 tccacctgag tatcaggatt tccctgaatc agggtataat ccagttcttt taaccagtct
    18361 tgcaatatca tgaaactata tttcctctct catacttaaa acagtcccgt gatctttcct
    18421 tcctcattca catcaatccc ctctgctgcc ggcttcttgg gaagtcccgg catggtcata
    18481 atggaacctg taagaacaac cacaaaaccg gctcctgcac atacataggc gtctcttacg
    18541 ttgatctgga agcctgcagg ccgtcccagc tttgtctgat catccgacag ggagtactgg
    18601 gtttttgcca tgcatacggg aagatttcca aagcccatct cttccatgcg ctttaacgcc
    18661 tttaaagctg ccggagtgta tgtcacgccg tccgctccgt atatctggga agcaatggtt
    18721 ccgatcttat cctttaagga agcttcattc tcatagagca catggaaatc agtgggacgg
    18781 ttttccaatg tgtttaatac cttttctgcc agggcaagcc caccctcgcc gccttttgcc
    18841 cagacctcag ccagagcaaa ttcacagcct ctttcctcac agaaatgctt aatataagca
    18901 tactccgctt ctgtatcgct tatgaaagaa ttaagggtaa ctataaccgg aacgccaaac
    18961 ttctggatgt tttcaatatg tttttccaga tttacaatgc ccttctttaa agcttccaga
    19021 ttttctacgg aaagctgatc cttcgggaca ccgccattat actttaaagc ccggacggtg
    19081 gctaccaaaa caaccgcatc cggctttaag cctgctttgc ggcacttgat atccataaac
    19141 ttctcagcgc ccagatccgc gccaaagcct gcttccgtaa caacaatatc gcagagctta
    19201 agagctgtct ttgtggctct tacactgttg cagccgtgtg cgatatttgc aaaggggccg
    19261 ccatgaacca acgcgcctgt atgctccaag gtctggatca ggttcggctt taaagcatcc
    19321 tttaaaagag ccgccatggc tccggtggca tgaagatctc ccgcagtcac cggctcgcct
    19381 gcaaaattat atgcaacgat aatgcgtgac aggcgctcct ttaaatcctt catatcatcg
    19441 gccagacaga ggattgccat gatctcggat gctacggtaa tgacaaaatg atcctctctt
    19501 accatgccgt cggcctttgc acccagaccg atcacaatat tacgcagcgc acggtcattc
    19561 atatcttcgc accgcttcca taaaatctgt cttggatcaa tgccgaggga atttccctgc
    19621 tggatatggt tatcaagaag agc
//
LOCUS       DBMDUH010000034        19135 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_1736_length_19135_cov_5.173479, whole genome shotgun sequence.
ACCESSION   DBMDUH010000034 DBMDUH010000000
VERSION     DBMDUH010000034.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 19135)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 19135)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19135
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1736_length_19135_cov_5.173479"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="ACZPCG_04660"
     CDS             complement(<1..596)
                     /locus_tag="ACZPCG_04660"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1284402.1"
                     /translation="MILDHIKAVIFDLDGTLVDSMWMWEDIDIEYLGRYGLSCPEDLQ
                     KAIEGMSFTETAVYFKERFGLPDSIEEIKEAWIAMSLEKYEKEVPLKPGAREFLEELA
                     ARGIPAGIATSNGREMVSAVLKSLDMEKYFREVVTACEVPFGKPAPDIYLEVAKRLGA
                     KQEDCLVFEDVPAGIMAGKRAGMTVYAVEDTFSKAMRK"
     gene            complement(593..1390)
                     /locus_tag="ACZPCG_04665"
     CDS             complement(593..1390)
                     /locus_tag="ACZPCG_04665"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007711427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIR1284403.1"
                     /translation="MAEKKKQNTGQMRLDKFLTEMGKGSRSQVKEFARKGRIQVNGQT
                     VKAADTKVNPEKDEIFLDGVPVSYARMEYFMLNKPQGTVSATEDVRYPTVVSLIGDAL
                     RGDLFPVGRLDLDTEGLLLITNDGALAHELLSPKKHVDKVYLAYIEGTLPKDAAEQMK
                     NGVILEDGVKTRPAELICLSREEGEQRLGRAPETAFAEAAPVTLTIHEGKFHQVKRMF
                     EALGCRVVFLKRLSMGTLYLDPALLPGEYRPLTQKELAGLKGEISEI"
     gene            complement(1438..2874)
                     /locus_tag="ACZPCG_04670"
     CDS             complement(1438..2874)
                     /locus_tag="ACZPCG_04670"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HIR1284404.1"
                     /translation="MTDVKMLPKDFLAGMKELLGKEYDQYIKSYEEEWKPGLRVNTLK
                     LSPEEFLEFSPWKLSPVPWTKNGFYYDSQDRPSRHPAYFAGLYYLQEPSAMTPAAMLP
                     VEPGDKVLDVCAAPGGKSTELAAKLKGEGLLVSNDISYSRARALLKNLELTGAANIAV
                     VSEEPEKLAEVWPEFFDKILVDAPCSGEGMFRRDDDMVKDWTSRGPEYYSPIQRRILE
                     QAVAMLKPGGMLLYSTCTFSRLEDEDNVNFLLESHPEMELVPFNLASVPGSADGIGLN
                     GCMRLFPHRMKGEGHFLALLRKKSEGSKKIPTPVGHPAKPGERHPNRAESRKIQGKKS
                     SASEDTGLRELEAFLRSCRISWEKERMFLHQEHVYYLPCGLETGLPLRFLRTGLLIGE
                     LKKGRFEPSQALAMALRIEEYPVVLNLSEEDKRVARYLKGETISLLPEEGPIKGWCLV
                     AYRGFPLGWAKGNNMTLKNKYYPGWRLQ"
     gene            2966..3931
                     /locus_tag="ACZPCG_04675"
     CDS             2966..3931
                     /locus_tag="ACZPCG_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition protein"
                     /protein_id="HIR1284405.1"
                     /translation="MKHSLGGLGAVFCLILLLACPALALTGARNGLLLWADVVLPTLL
                     PFMICSGMIVALNGVPLLTKPLFPFLSRFMGLSQPGSFVFITGLLCGYPMGARMDSRF
                     LEEGRIELNEARTLLAVSNHPSPMFILGYICVQAASLFSSSNACPPWIFLTALYLPIL
                     PVSFLAKKCYHLNQEASLCRQDNYKSEHTRVSFDAHLMDCLETMEKIGLYIMIFSILA
                     LYLSALPDSLLPLTLRCALLGAVEITTGIRAISGSVPGFAGALLITAAGAFGGISGIF
                     QTKSVLKSAGLSIRHYILWKFFHSVLSCVAFALCFFINQSLQIGI"
     gene            complement(3928..4626)
                     /locus_tag="ACZPCG_04680"
     CDS             complement(3928..4626)
                     /locus_tag="ACZPCG_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vacuolar family H+-ATPase subunit H"
                     /protein_id="HIR1284406.1"
                     /translation="MSRIEQLISEIEEYIDSCKSQALSKSKIIVNKEEMEELLVELRM
                     RIPDEITKYQKIISQQQTILNDARSQSDAMLSEAKAQADAMIAQATEQANSMVSQASE
                     EANSMVAQANEQTTEMINEHEIMQRAYSHAEEVISQANIQAQAIVDAAVNDANNIRQG
                     SIQYTDDMLRSLQTILNHTMDNAKGRFDAFMSSMQSSYDIVSANRNELSGGIVKEENA
                     EAAAAVENQETQAD"
     gene            complement(4640..5137)
                     /gene="coaD"
                     /locus_tag="ACZPCG_04685"
     CDS             complement(4640..5137)
                     /gene="coaD"
                     /locus_tag="ACZPCG_04685"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HIR1284407.1"
                     /translation="MKIAVYPGSFDPVTLGHLDIIKRTASMFDKVIIGVLNNRAKSPL
                     FTARERVELLEEVTGDIPNVEVQAFHGLLIDFVRQNDAKVIVRGLRAITDFEYELQMA
                     QTNRVIAPEIDTIFLTTNLKYSYLSSSIVKEIAEYDGDISGFLHPATARRVREKLEEI
                     KKVQG"
     gene            complement(5138..5695)
                     /gene="rsmD"
                     /locus_tag="ACZPCG_04690"
     CDS             complement(5138..5695)
                     /gene="rsmD"
                     /locus_tag="ACZPCG_04690"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HIR1284408.1"
                     /translation="MRVIAGSARRLLLKTIEGLDTRPTTDRIKETLFNMLQPQIPGCF
                     FLDLFSGSGAIGIEALSRGARKAVFIENNPKAVECIRENLSRTHLEEGALVLESDVIA
                     GLKRLEGRNYRFDLIFMDPPYGMELEKRVLEYLSGSPMVTEDTQIIIEASKETDFGWL
                     EEAGYHMTKEKIYKTNKHIFAGKGE"
     gene            complement(5852..6718)
                     /gene="hflC"
                     /locus_tag="ACZPCG_04695"
     CDS             complement(5852..6718)
                     /gene="hflC"
                     /locus_tag="ACZPCG_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003499269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0098797 - plasma membrane protein complex
                     [Evidence IEA];
                     GO_process: GO:0052547 - regulation of peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HIR1284409.1"
                     /translation="MKKVGIIAGFAAAAAAVLLGASLVVTYPNEYKLIRQFGEIVRVV
                     ETPGVSLKVPFLQTGTSIPKSLQIYDIPQSDVITKDKKTMIADAFVLWKISDPVLFTR
                     HLNGQVAQAQSRIAASVFSTMKSVISNMDQAEIIENRDGKLALDISSGIGDSLDGYGI
                     QVMAVETKSLDMPDDNKQSVYDRMISERNNIAASYTAQGNSSAQKIKNDTTKEVSVMK
                     SKAAAEAERIRAEGEAQYMQILSNAYNDASKADFYNFVRSLDAAKASLTSGNNTLILD
                     KTSPIAQIFYGY"
     gene            complement(6715..7848)
                     /gene="hflK"
                     /locus_tag="ACZPCG_04700"
     CDS             complement(6715..7848)
                     /gene="hflK"
                     /locus_tag="ACZPCG_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003499271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HIR1284410.1"
                     /translation="MKKHPLGQNPDFRKLFQQLNGQYSGKKPEDGQGEKDGTPEPERF
                     DSNGNPVKKKKKRRRFARGGKVAAAAVILILLGTNSYYILDEENYAVVSTLGSAQAVS
                     QAGLHFKIPFIQNVRRVSKGIKGMPIGYDPETGTSDESESIMITKDFNFVNTDFYLEY
                     MVNDPVKYLYASSEPVATLKMLAQSYIRDTVGTHNVDDVITTGKSEIQSEIKEKLTNR
                     MMEEDIGLAVVNITIQDAFPPTTEVLNAFKNVENAKQGKETAINNANKDRNEKIPQAE
                     AQCDQIIKDAEAEREARINEAQGQVSRFEQMYAEYTKYPLITRQRMFYETMEEVLPHM
                     KLYIVDESGTQKLLPLDRFAEAMEQQTASGQPSGEITIEEAQE"
     gene            complement(7953..9332)
                     /locus_tag="ACZPCG_04705"
     CDS             complement(7953..9332)
                     /locus_tag="ACZPCG_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006779645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1284411.1"
                     /translation="MRQLMIRTGEMLRDRRFLKKALMIACPVALQGMLNTVVNMVDTL
                     MIGGLGSSAIAAVGLANKYFFVFSLLVFGIVSGAGILAAQYWGNGDVKSIRKVLGLSI
                     LLAAAGALVFVLPGLIWPHQVMRIFTTSESSIELGIKYLRIAVLTYPFIAMTNVYVAM
                     LRAVNKVVYPVVCSCIAIAINVVLNYVLIFGKFGAPQMGVAGAACATLIARCIEVSLV
                     LLYVYGKKLPLACGFKGLFGWSRDFILRFADTSAPVIANEFMWGLGTTLYSLAYGRMG
                     DDAVAAITIATTIQDIFVVLFQGLSAATAVILGNELGAGKIKRAEKYATQFFILQFII
                     TMAADFVMVAGRWQIIGIYTITPQVAMDVNLCLLVFALYMPAKMFNYVNVVGVLRSGG
                     DTKMCLFLDTSGVWIIGVPLAFLGALVWHLPIYLVYALVLSEEIYKMILGYWRYRQKK
                     WLRNLALDL"
     gene            complement(9292..10197)
                     /locus_tag="ACZPCG_04710"
                     /pseudo
     CDS             complement(9292..10197)
                     /locus_tag="ACZPCG_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007711810.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
     gene            complement(10250..10612)
                     /locus_tag="ACZPCG_04715"
     CDS             complement(10250..10612)
                     /locus_tag="ACZPCG_04715"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA];
                     GO_process: GO:0016075 - rRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HIR1284412.1"
                     /translation="MIIRRGDIFYADLRPVVGSEQGGVRPVLIIQNDVGNRHSPTVIC
                     AAITSRMNKAKLPTHVELKARNRSLIRDSVILLEQVRTIDKQRLREKICHIDGEMMRR
                     VDEAMRISLELGTRERSF"
     gene            complement(10698..11888)
                     /gene="alr"
                     /locus_tag="ACZPCG_04720"
     CDS             complement(10698..11888)
                     /gene="alr"
                     /locus_tag="ACZPCG_04720"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR1284413.1"
                     /translation="MRPYSRVYATVNLDAVVSNMKAMREALPASTAIMGVVKADGYGH
                     GAVPVAWAMDPYVEGYAVSNIDEGINLRRHGIQKPILILGVTHRQRFEELLQYKITPA
                     MFRLKEAKELSCLAKERGLTAKIHLALDTGMSRIGMAPSRDAAKEAAGMASLPGIEIE
                     GLFTHFARADETDKTFYREQYDKYLAFLGYLEEYGVNIPIRHCSNSAGIVEDLDSNHM
                     DMVRAGICIYGLYPSDEVDRSRIRLTPIMEIKSFITYIKEIGPKTPVSYGSTFVSDKT
                     MRVATIPTGYADGYNRALSNKGFVLIRGKRARILGRICMDQFMVDVTDIPEAKEDDEV
                     TLIGRDGEEEITVEEMAACSGGFHYEIICDIGKRVPRVYIKDGAIAGTKDYFDDRYEG
                     FEEP"
     gene            complement(11885..13471)
                     /locus_tag="ACZPCG_04725"
     CDS             complement(11885..13471)
                     /locus_tag="ACZPCG_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007711846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HIR1284414.1"
                     /translation="MRTLLTGRKMKAVDHYTIHEVGIPSLVLMERAALAVADCALKAA
                     RAFAAHSPVWALCGVGNNGADAAAAARMVYLKGLPAVIFLASSEEKATEELKLQLSIA
                     RKLGIPVKKWELMKEEAENCGILFDGLFGVGLSREIQGEYRACMELAAEILKGNGKTK
                     PWCIAVDIPSGIHSGTGAVMGIALKADETVAFGWEKIGTAVYPGRDYAGNVTVADIGF
                     PEEALRAEPEEEDPISMAYACEESDLNRVPERKAYSNKGTFGRVLIAAGSKNMSGAAY
                     LSALGAYRAGAGLVKILTVEENRTVLQTLLPEAIISSYRPEELLGGREDFKERIEAEM
                     NWADVVVLGPGLGSEPYVEYLVEDILTSAFVPVIIDADGLNTIASHPYLTSYYTENIV
                     ITPHLGEMARLTSQKISEIQADLLAAAFSYASHYGITCVLKDAAAVTAGKEGTLYINT
                     SGSSAMAKAGSGDVLTGILAGLMALGMEEEEAAALGVYIHGLAGERAAGNKLHSLLAS
                     EIAEAAGAFMDRQKEEIKTK"
     gene            complement(13473..13856)
                     /gene="acpS"
                     /locus_tag="ACZPCG_04730"
     CDS             complement(13473..13856)
                     /gene="acpS"
                     /locus_tag="ACZPCG_04730"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HIR1284415.1"
                     /translation="MILGVGTDLIEIKRMEKACRRPHFVARTFTETESRQAKGSASKL
                     AGSFAVKEAVAKMFGTGFRGFMPGDIEVLRDELGKPYVNLYGGAKKRSEEMGLTQIHV
                     SISNTEDFAMAFAVGEKETSAERRT"
     gene            complement(13858..14523)
                     /locus_tag="ACZPCG_04735"
     CDS             complement(13858..14523)
                     /locus_tag="ACZPCG_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002600292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HIR1284416.1"
                     /translation="MERKEISKAVISRLPRYYRYLGELIEEGVERISSNELSNRMKVT
                     ASQIRQDLNNFGGFGQQGYGYNVKYLYSEIAKILGIDQQHNLIIIGAGNLGQAIANYT
                     NFERRGFVIKGMFDINPKLIGLVIRGIEIRSVDDLESFIKENEIQIAALTIPKTKAPE
                     IADRLVAAGIKAIWNFAHTDLVVPEDVVVENVHLSESLMRLSYRVCSMLDRDEKNERT
                     GSK"
     gene            14699..16678
                     /gene="abc-f"
                     /locus_tag="ACZPCG_04740"
     CDS             14699..16678
                     /gene="abc-f"
                     /locus_tag="ACZPCG_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008396120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HIR1284417.1"
                     /translation="MILSCSNISRSFGDNHILKRVSFHIEEHEKAAVVGINGAGKSTL
                     LKIIIGDLAPDEGSVTWAKGASIGYLAQHQDLEGAETIYDALLEVKRPILEMEERLRN
                     LEQSMKSASGEELEAMLSEYSRLNHAFELENGYACRSEITGVLKGLGFSEDEFSKPIQ
                     ALSGGQKTRVSLGKLLLTKPDILLLDEPTNHLDMESIAWLETYLKGYSGSVIIVAHDR
                     YFLDRVVTKVIELDNGTATVFSGNYSAYSDKKAMLRDAQIRAYLNQQQEIRHQEAVIA
                     KLKSFNREKSIRRAESREKMLDKIERLEKPVEINDSMDIRLEPDVVSGNDVLTVTDLS
                     KSFDTQTLFTHGSFEIKRGERIAVIGNNGTGKTTLLKIINGLIPADAGEIRLGAKVHI
                     GYYDQEHQVLHMDKTLFQEIQDTYPNMNNTQIRNTLASFLFTGDDVFKLIKDLSGGER
                     GRVSLAKLMLSDANFLLLDEPTNHLDITSKEILESALNRYTGTVLYVSHDRYFINRTA
                     TRILDLTGQSFINYIGNYDYYLEKKEDVEAAFFAGRGSEAPKSALGRPADAGTGASSG
                     TAASSSASDTGAKLDWKAQKEEQARIRKRQNELKKTEDAIHQLETRDSEINELLALEE
                     VYTDVSRLMELNKEKDSISEKLEKLYELWEALAEE"
     gene            16675..16905
                     /locus_tag="ACZPCG_04745"
     CDS             16675..16905
                     /locus_tag="ACZPCG_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284418.1"
                     /translation="MKLKRILALLGVLLLAGLVLLFFILALTGASKNTLMAVAFSILF
                     FSVLFYAMALMARVLKHGDDPGQEEDERKKTL"
     gene            complement(17212..18063)
                     /locus_tag="ACZPCG_04750"
     CDS             complement(17212..18063)
                     /locus_tag="ACZPCG_04750"
                     /inference="COORDINATES: protein motif:HMM:NF025620.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HIR1284419.1"
                     /translation="MATGNNILYVFITGGDYTLSSRDDGIHADDGAAISGGSIKISES
                     CEGIEGLTIDITGGQIDITASDDGVNAAGGRDGSSMEKGQDPFVSMEGAYISISGGSL
                     RINASGDGISSLDYNGKAVVTGGIFASSGASGMAQNFDPSSTQGIIMVTLNPQEAGTQ
                     IALKNSDGTELLSWKAEKEYSSVLISSPDIRQGERYSLTAGTEEKTITMDSLVYGTDI
                     PENGKAPVGSETPKGGRGPENGEVPTGGRAPMKREPAENGKRPGEMDNKPGMGEKPDN
                     ENKKEIQ"
     gene            18285..18629
                     /locus_tag="ACZPCG_04755"
     CDS             18285..18629
                     /locus_tag="ACZPCG_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7768 domain-containing protein"
                     /protein_id="HIR1284420.1"
                     /translation="MKRPLAYLTAAWSGQPEIDMELAAGYCRLAYEAGFSPICPLIYL
                     PLFLDDSVPEEHKAGIDMARDMLRRSHTLIVYGETVDEDVKNDIAVAGRLGIAATTLE
                     GVLAVKGHGRNA"
     gene            18622..18759
                     /locus_tag="ACZPCG_04760"
                     /pseudo
     CDS             18622..18759
                     /locus_tag="ACZPCG_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005927707.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
     gene            complement(18755..19135)
                     /locus_tag="ACZPCG_04765"
                     /pseudo
     CDS             complement(18755..19135)
                     /locus_tag="ACZPCG_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         4544 a         5164 c         4091 g         5336 t
ORIGIN      
        1 tctttccgca tggctttgga aaatgtatct tccactgcgt acaccgtcat cccggctctc
       61 tttccagcca tgattccagc cggaacatcc tcaaacacca ggcagtcctc ctgctttgct
      121 cccagccgtt tcgctacctc cagatagata tctggagccg gttttccaaa gggtacctca
      181 caggccgtta ccacctcacg gaaatatttt tccatatcaa gggattttaa tacagcggat
      241 accatttctc tgccgttgct ggtggcaatg cctgcgggaa tccctctggc tgcaagctcc
      301 tccagaaatt cccgtgctcc cggttttaga ggaacttcct tttcatattt ttccagagac
      361 atggcaatcc aggcttcctt gatttcttct atagagtcag gaagtccgaa gcgctcctta
      421 aaataaacag ccgtctctgt aaagctcatg ccttcaatgg ctttctgcag atcctccggg
      481 caggacaggc cgtaccgtcc cagatattcg atgtcaatat cctcccacat ccacatggag
      541 tctaccagtg ttccatccag gtcgaaaatg actgctttta tgtgatccag tatcatattt
      601 ctgatatttc tcctttcaga ccggccagtt ccttttgggt aagcggcctg tattctcccg
      661 gcaaaagcgc cggatccaga taaagcgttc ccatggacag ccgctttaaa aatacgaccc
      721 tgcagcccag agcttcaaac atccgcttta cctgatggaa tttcccctca tggatggtca
      781 gggtcacagg agccgcttct gcaaaggctg tctccggcgc acgtcccaaa cgctgttccc
      841 cctcttctct gcttaaacag ataagctctg ccggtcgggt ctttactcca tcctctaaaa
      901 tcacgccatt cttcatctgt tctgcggcat ctttagggag agttccctcg atataagcca
      961 gatagacctt atccacatgt tttttcgggg aaagcagttc atgggccaat gctccgtcat
     1021 tggtaatcaa gagcaggcct tctgtatcca gatccaaccg tccaaccgga aagagatctc
     1081 ccctgagggc atccccgatc aggcttacta cagtaggata acggacatcc tctgtggcag
     1141 atacggtccc ctgaggctta tttaacataa aatactccat ccgggcatag cttaccggca
     1201 caccgtcaag gaaaatctca tccttttccg gatttacctt tgtatctgct gcctttacgg
     1261 tctgtccatt gacctggatc cggccctttc tcgcaaactc ctttacctgg ctgcggcttc
     1321 cctttcccat ttctgtgaga aatttatcca gacgcatctg tccggtattc tgttttttct
     1381 tctctgccat atggatttta ctcctgtttt tcttatccgc cgctggcagc ttcacagtta
     1441 ctgtaaacgc cagccgggat agtatttatt tttcagtgtc atattgtttc ccttggccca
     1501 gcctagtggg aaacctctat aggccaccag gcaccagcct tttatggggc cttcctccgg
     1561 cagcaggctg atcgtttccc ctttcaggta gcgcgccaca cgcttatctt cctcagaaag
     1621 atttaaaacc actggatatt cctctatgcg cagcgccatt gccagtgcct gtgagggctc
     1681 aaaacggccc ttcttcagtt ctccgatcag caggccggtg cgcagaaaac gtaaaggcag
     1741 gcccgtttcc agtccgcagg gcagatagta tacatgttcc tgatggagga acatacgttc
     1801 cttttcccag gaaatcctgc atgaacgtaa aaatgcttcc agctcccgaa ggcctgtatc
     1861 ctcagaagcc gaagactttt tgccctggat ctttctgctt tcggctctgt tgggatgacg
     1921 ttctcctggt ttggcaggat gaccgaccgg ggttggtatc ttttttgatc cttcactctt
     1981 ttttcttaaa agcgccagaa aatggccttc ccccttcatc cggtggggaa aaaggcgcat
     2041 acagccgttc agtcctatgc cgtctgcgga tccgggtaca gaggcaagat tgaaaggcac
     2101 cagctccatt tccgggtgcg attctaaaag aaagtttaca ttatcctcat cctccagcct
     2161 tgaaaaggtg caggtagagt aaaggagcat accgcccggt ttcagcatag ccacggcctg
     2221 ctctaagatc cggcgctgga tggggctgta atattcagga cctctgctgg tccagtcttt
     2281 taccatgtca tcatcccgcc ggaacatccc ctcgccggaa cagggagcat ctactaaaat
     2341 tttatcaaaa aattccggcc agacctctgc cagcttttcc ggttcctcgc tgaccactgc
     2401 aatattggca gctcctgtta attccagatt tttaagcagg gctcttgccc tggaatagct
     2461 gatatcattt gataccagaa ggccctctcc cttaagcttt gccgccagct ccgtactctt
     2521 tcctcccgga gccgcacata catccaatac cttatctccc ggctccactg gaagcatggc
     2581 cgccggcgtc atggcgctgg gttcctgaag gtagtacagc cctgcaaaat aggcaggatg
     2641 tctggatggc cgatcctggc tgtcataata aaacccattt ttcgtccaag ggactggaga
     2701 aagtttccag ggagaaaatt ctaaaaattc ctcaggagaa agcttaagcg tgttcactct
     2761 cagtcccggt ttccattctt cctcatagct ttttatatac tggtcgtatt cctttcccaa
     2821 aagttctttc atcccggcaa gaaagtcttt tgggagcatt ttcacatctg tcatcttttt
     2881 gacctttcaa tgtttttcca tattggttat attactgtac ttcttccata tactgataca
     2941 aaactcccac cctctaaggt tcttcatgaa acactccctc ggcggtttgg gggcagtctt
     3001 ctgcctcatc cttcttcttg cctgcccggc tctggccctc acaggagcca gaaatgggct
     3061 tcttctctgg gcggatgtgg ttcttcccac ccttctgccc tttatgatct gctccggcat
     3121 gatcgtggcc ctaaacggcg ttcccctgct cacaaagccc cttttccctt ttttatcccg
     3181 ttttatgggc ctgtcccagc cgggaagctt tgtctttatc acgggccttc tatgcggata
     3241 ccccatggga gcacggatgg acagccgttt cctggaagaa ggacggattg aacttaatga
     3301 agccagaacg cttcttgccg tcagcaacca ccccagcccc atgtttatcc tgggttatat
     3361 ctgcgttcag gccgcatccc ttttttcttc ttcaaatgcc tgtccgccct ggatctttct
     3421 caccgctttg tatctgccca tacttccggt ttcttttctg gcaaaaaaat gttatcatct
     3481 gaatcaggaa gcgtctcttt gcagacagga taactataaa tcggagcata cacgcgtttc
     3541 ctttgacgcc cacttaatgg actgtctgga aaccatggag aaaatcggcc tttatattat
     3601 gattttttcc attttggctt tgtatctgag tgcacttcca gattcccttc tccctcttac
     3661 gctaagatgc gccctcttag gcgcggtaga aatcactaca ggcatccggg cgatctcagg
     3721 ctctgttccc ggctttgccg gagcccttct tatcacagca gccggagcct ttggtgggat
     3781 ttccggaatc tttcagacaa aaagtgtact aaaaagtgcc ggactgtcta tccggcacta
     3841 tattctgtgg aagtttttcc acagcgtcct ttcctgcgtg gcctttgctc tgtgcttttt
     3901 cataaatcaa agcctgcaga tcggtattta atcagcctgt gtttcctgat tctccacagc
     3961 ggccgcagcc tctgcattct cctctttcac aatccctccg gacagctcat tgcggttggc
     4021 agaaacaata tcatagctgg actgcatgga actcataaat gcgtcaaaac gcccctttgc
     4081 gttgtccatg gtgtggttaa gaatggtctg caggctgcgg agcatatcat ccgtatactg
     4141 aatggagccc tgtcggatat tattggcgtc attcactgcg gcgtctacga tcgcctgagc
     4201 ctggatattg gcctgtgaaa taacctcctc cgcatggctg taggcccgct gcatgatctc
     4261 atgctcattg atcatctctg tcgtctgctc attagcctga gctaccatgg agtttgcctc
     4321 ctctgaagcc tggcttacca tggaatttgc ctgctctgtc gcctgggcga tcatggcgtc
     4381 tgcctgagcc tttgcctcag aaagcatggc gtcggactgg gatctggcgt catttaagat
     4441 ggtctgctgc tgactgatga tcttctgata ctttgtgatt tcatccggga tccgcatacg
     4501 aagctctacc aaaagctcct ccatctcctc tttgttcaca atgatcttgc ttttggacag
     4561 cgcctgagac ttgcagctgt caatatattc ttcaatctcg cttatcaact gttcaattct
     4621 gctcatatta cgatctcctt catccctgta ctttttttat ctcttccagc ttttctctta
     4681 ctcgtctggc tgtggccgga tggagaaagc cgctgatatc accgtcatac tccgcgattt
     4741 ccttcacaat gctggaactt aaataggagt atttcaggtt ggtcgtcaaa aatatggtat
     4801 cgatctcagg ggcaatgacc ctgtttgtct gggccatctg aagctcatat tcaaaatccg
     4861 tgatggcgcg aagacctctt acgatcacct tggcatcatt ttggcggaca aagtcgatca
     4921 gaaggccatg gaacgcctga acttccacat tgggaatatc tcctgtcact tcctctaaca
     4981 actctacccg ctctctggcc gtaaacaggg gcgattttgc ccgattattt aatactccga
     5041 taattacctt atcaaacatg gatgcggtac gcttaataat atccaaatga cccagagtga
     5101 ccggatcgaa gctgcctgga tatactgcaa ttttcattta ttctcccttg ccagcaaaga
     5161 tatgcttgtt ggtcttgtat attttttctt ttgtcatatg gtaaccagct tcctctaacc
     5221 agccaaaatc cgtttctttg gacgcttcta ttatgatctg tgtatcctct gttaccatcg
     5281 gtgaacccga aagatactcc agaacccgtt tttcaagttc cattccatag ggcggatcca
     5341 taaaaatcag gtcaaaacga taatttcttc cttccagccg cttaagtcct gcaattacgt
     5401 cagattccaa aacaagagcc ccctcttcca ggtgggtacg gctcagattt tcgcggatac
     5461 actccaccgc ttttggatta ttttcaataa agactgcctt tctggctcct ctgcttaagg
     5521 cttcaatccc gatggcaccg cttccggaaa aaagatccag aaaaaagcat ccgggaatct
     5581 ggggctggag catattaaaa agtgtctctt tgatccggtc tgtagttgga cgtgtgtcca
     5641 gaccttctat tgtttttaaa agcagccttc tggcactgcc tgcgataact ctcatggaaa
     5701 aactcctgta attaagcaat tttcattcca aacaagtata atatgaaatg agctgtaata
     5761 caaggttttt ttggaatttt ctaaaaagag cagacacagt ttcccatgtc cgctcgtttg
     5821 catctgtttc tttcctatcc tgccctcaaa atcagtaccc gtaaaagatc tgggcaatgg
     5881 gtgaagtctt atcaaggatc agcgtattat ttccgctcgt aagagacgcc tttgccgcat
     5941 ccagagagcg gacaaaattg taaaaatctg ccttgctggc gtcattatag gcattagata
     6001 aaatctgcat atactgggct tctccttctg cacggatgcg ttccgcctca gcggccgcct
     6061 tggatttcat cacagacacc tcttttgtgg tgtcattttt aatcttctgg gcagaggaat
     6121 ttccctgagc cgtataggaa gcggcaatgt tgttccgctc agagatcata cggtcgtaaa
     6181 cagactgctt attgtcatcc ggcatatcta aagacttggt ttctaccgcc attacctgaa
     6241 tgccatagcc gtccagagaa tctccaattc cgctgctgat atcaagagcc agctttccgt
     6301 ctctgttttc aatgatctcg gcctgatcca tgttggaaat gacagatttc atggtggaaa
     6361 aaacagaagc tgcaatacgg gactgggcct gagctacctg tccgtttaaa tgtctggtaa
     6421 agagcaccgg gtcagagatc ttccacagta caaaagcatc ggcgatcatg gtctttttat
     6481 cctttgtaat tacatccgac tgggggatgt catagatctg aagggacttt ggaatggaag
     6541 tccctgtctg aagaaaaggc acctttaagc tcactcccgg agtttccact acccggacga
     6601 tctcgccgaa ctggcggatc agcttatact cgttgggata ggtcaccact aaggacgctc
     6661 ccagtaaaac cgcagcagcc gccgcagcaa aacctgctat gataccgact tttttcattc
     6721 ctgcgcctcc tctatggtga tctctccgga tggctggccg gaggctgtct gctgttccat
     6781 ggcttctgca aaacgatcca ggggcagcag cttctgagtc ccgctttcat ctacaatata
     6841 aagcttcata tgaggcagaa cctcttccat agtctcataa aacatacgct gtcttgtgat
     6901 cagaggatat ttggtatatt ccgcatacat ctgttcaaat cgcgacacct ggccctgggc
     6961 ctcattgata cgggcttccc gttctgcctc tgcatccttt ataatctggt cgcactgagc
     7021 ttctgcctga ggaatttttt catttcggtc cttattggca ttattgatgg cagtttcctt
     7081 tccctgcttg gcattttcca cgtttttaaa ggcattcagt acctctgtgg taggaggaaa
     7141 ggcgtcctga atggtaatat ttaccactgc aagaccgata tcctcttcca tcatacggtt
     7201 tgtaagtttt tccttgattt ctgattggat ttcagatttt cctgtggtaa ttacatcgtc
     7261 tacattgtgg gttcccaccg tatcgcggat ataggactgg gccagcattt ttaaggtggc
     7321 aacaggctct gaggatgcgt agagatattt gaccgggtca tttaccatat attccaaata
     7381 gaaatccgtg tttacaaagt taaaatcctt ggtgatcatg atagattcag attcatcact
     7441 cgttccggtc tcagggtcat agccaatcgg cattccctta atgcccttgg atacccggcg
     7501 aacattctgg ataaatggga ttttaaaatg aagccctgcc tgagatactg cctgggcact
     7561 tcccagcgta ctgacaactg cataattttc ttcatccaga atatagtagc tgttcgtacc
     7621 cagaaggatc agtataacag ccgcagctgc cacctttccc cctctggcaa aacggcgtct
     7681 ttttttcttc ttttttaccg ggttgccgtt ggaatcaaag cgctccggct ccggcgtccc
     7741 gtccttctcc ccctgaccgt cttccggctt tttaccgctg tactgtccat tcagctgctg
     7801 aaaaagcttc cggaaatcag ggttttgtcc caatggatgt tttttcatgt aactcctcct
     7861 tatctgcccg acataggtca ctcatccccc gaaaaatata taagaggatt ataacagata
     7921 gaaggctggt tttcaaccaa aatgacagtc ttttacagat ccaaagccag atttcttagc
     7981 cattttttct gacggtaccg ccaatagccc aaaatcattt tatagatttc ctcagaaagt
     8041 accagagcgt ataccagata aatcggaaga tgccatacaa gcgctcccag aaaagccagc
     8101 ggaacaccga tgatccacac gccgctggtg tccaaaaaga ggcacatttt cgtgtcgccg
     8161 ccgcttctta aaacacctac cacatttaca tagttaaaca tttttgcagg catatagagg
     8221 gcaaatacca gcaggcaaag atttacatcc atggctacct gaggggtaat ggtataaatc
     8281 ccgatgatct gccaccgtcc tgccaccatc acaaagtcag ctgccatagt tatgataaac
     8341 tgcaggataa aaaactgggt ggcatatttt tctgctctct ttatctttcc cgcgccaagt
     8401 tcatttccaa gaatgacggc tgtagccgcg cttaagccct ggaacagcac tacaaaaata
     8461 tcctggatcg tggtagcaat ggtaatggcc gccactgcgt catctcccat acggccatat
     8521 gccagagagt acagtgtagt ccccagtccc cacataaatt catttgcaat aaccggagcc
     8581 gatgtatctg cgaaacggag gataaaatcc ctgctccatc caaacaggcc tttaaagccg
     8641 caggccagag gaagcttttt gccgtaaaca tataggagca caagactgac ctcaatgcac
     8701 cgggcgatca aagtcgcgca ggcagcgcct gccacgccca tctgcggagc accgaatttt
     8761 ccaaagatca gcacatagtt taaaacaaca ttgatagcaa ttgcaataca gctgcatacc
     8821 acaggatata caaccttatt tacagcccgc agcatggcga cgtacacatt tgtcatggca
     8881 ataaaagggt atgtgagcac ggcgatccga agatatttta tgcccagttc aatgctgctc
     8941 tcactggttg taaagatccg catcacctga tggggccaga tcaatcccgg gagcacaaat
     9001 acaagtgctc cggctgcggc caggagaatg gataagccca atactttccg tatgctcttt
     9061 acatctccat taccccagta ctgggccgct aaaataccgg ctccgctgac aatgccgaat
     9121 accagcagtg aaaatacaaa aaaatactta tttgcaagtc ccacggcggc aatggcgctg
     9181 gagccaaggc ctccgatcat cagagtatct accatattta ctactgtatt aagcataccc
     9241 tgcagtgcca cagggcatgc gatcatcagc gcttttttaa gaaatctccg gtcacggagc
     9301 atttctcccg tgcggatcat cagctgtctc atcgttcgtt tccattcctt cctcgttcat
     9361 tctgtttttc ctgtcttcca gcagtttttt tgcaagtacg ctttcaattt tcttatttcc
     9421 gatcgttaac acctgaaaat gccagccgga ctggtcgatg gagggctttt cgccctctgc
     9481 cggaatccgg tctaaccggg atgtaagaaa accgtttaat gtatcgtact cctcctcgtc
     9541 ctctcccagg ctgagtttta gcgcctctga aacctcatca aagggagcca gtccgctgat
     9601 aagccaggaa ccgtcctctc tttcggtcat atagtgctct tcctcgtcat attcatccat
     9661 gatgttcccc acgatttctt ctaaaatgtc ctccatggtc accaggcctg cggtttgtcc
     9721 atactcgtct accacgattt ccatatggat cttttccgac tgcatttctt taaaaagaga
     9781 atcaatcttt tttgtctcag ggataaaagc cgcctctctt aaaagtcccg gcactttacg
     9841 gatttcctgt tccctgaatt ctgctttgcg ggccagcatg gccgcatctc tcagatgaag
     9901 gatcccgata atatcatcca tatcctcgcc atatacggga taccgtgtat ttatgccctc
     9961 atccagcata aaatcaaggg cagcctccag cttcatggaa ccgtcaagag ccgtgatccc
    10021 ggttcggtga gtcataatat cttctgccgt tgtgtcacca agctggaaaa tattggatat
    10081 catttctgtt tcatctgctt ccagcacccc ctgctcatgg ccttcattga ccatggtcat
    10141 aatatcttct tccgttacat cctcataacc gtgtaacttg atcccaaagc gccttaaagc
    10201 tgcaaaaagc ttttttccag gcccccagct tccatcctcc atagtaaatt caaaaactcc
    10261 tttctctcgt tcccagctcc aggctgatcc gcatagcctc atctacccgt ctcatcattt
    10321 ccccgtcaat atgacagatt ttttctctca gccgctgttt atcaatggtg cgtacctgct
    10381 ccagtaaaat gacagaatcc ctgatcagac tccggtttct ggctttcagc tctacatgag
    10441 taggcagttt tgccttattc atacgggagg tgatcgccgc acagattacc gtaggactat
    10501 gtcggtttcc cacatcattc tgaatgatca gcaccggacg cacacctccc tgttcagagc
    10561 ccacaaccgg gcgaagatcc gcataaaaga tatctccccg tctgatgatc atagctttcc
    10621 cctcccatgt actgatacac tatcagcata tgtaggggga cagtccaggg agcgtgtctg
    10681 tacctcttat aaatatctta tggctcttca aacccttcgt agcggtcatc aaaataatcc
    10741 tttgttcccg caatcgcacc gtcttttata tagactcggg gcacccgctt tccaatatcg
    10801 cagatgatct catagtggaa accacctgag caggcagcca tttcttctac tgtgatctct
    10861 tcttccccat cccttccgat caaagtaacc tcatcatcct cttttgcctc agggatatcc
    10921 gtcacatcga ccataaactg atccatgcag atccggccaa ggatcctggc ccgctttccg
    10981 cggataagga caaaaccttt attggacaaa gcgcggttgt aaccgtccgc atatccggta
    11041 ggaatggttg ccacccgcat agtcttatct gatacaaagg tgctgccgta gcttactggc
    11101 gtttttggtc ctatttcctt aatataggtg ataaagctct taatttccat tataggcgtc
    11161 agtcgaatcc ggcttcgatc cacttcatcc gatggataaa gaccataaat acagattccg
    11221 gcacggacca tatccatatg gtttgaatcc agatcttcta cgatcccggc gctgttggaa
    11281 cagtggcgga tgggaatatt gaccccatat tcctccagat aacctaaaaa ggccagatat
    11341 ttgtcatact gctccctgta aaaggtcttg tctgtctcat ctgctcttgc aaaatgggta
    11401 aaaagcccct cgatttcaat gcccggaagg gacgccatcc cggctgcctc ctttgcagca
    11461 tccctgctgg gagccatgcc gatccggctc atgcctgtat caagggccag atggattttg
    11521 gccgtaaggc ctctttcctt tgccagacag gataattcct tggcttcttt taaacggaac
    11581 atagccgggg tgatcttgta ctgcaaaagc tcctcaaacc gctgtctgtg ggtcacacct
    11641 aaaatcagga tcggtttttg gatcccatgg cggcgcagat tgatcccttc gtctatatta
    11701 gataccgcat aaccttccac atacggatcc atggcccagg ccaccggaac tgctccatgg
    11761 ccgtagccgt ctgcctttac gacacccata atggctgtag aggccggaag ggcctctctc
    11821 atagctttca tattagaaac aacggcatcc agatttaccg ttgcatatac tctgctgtaa
    11881 ggtctcattt tgttttgatt tcctccttct gccgatccat aaaggccccg gctgcctcag
    11941 caatctctga tgcaagcagg ctgtgaagct tatttccggc tgcacgctct cctgccagcc
    12001 catgaatata aacgcccaaa gcggctgctt cctcttcctc cattcccagg gccatcagcc
    12061 cggccaggat gccggtaagc acatctccgg atccggcctt tgccatagcg ctgcttccgc
    12121 tggtattgat atataaagtc ccctcttttc ctgcggtcac ggctgccgca tctttaagaa
    12181 cgcaggtaat cccgtagtgg gatgcatagg aaaatgccgc agccagaaga tcggcctgaa
    12241 tttcactgat tttctggctt gtaagccgcg ccatttcccc caaatgggga gtgatcacaa
    12301 tattttccgt atagtaggag gtcagatagg ggtgagatgc aatggtattt aagccgtccg
    12361 catcaatgat cacaggcaca aaggcgcttg taaggatatc ctctaccaga tattccacat
    12421 aaggctcgct tccaagacca gggcctaaaa ccaccacatc tgcccagttc atctcagcct
    12481 cgatccgttc cttaaaatcc tctctcccgc ctaaaagctc ttccggacga taagaagaaa
    12541 taatggcttc cggaagaagg gtctgtaaaa ccgtcctgtt ttcttccacg gtcaggattt
    12601 ttacaagtcc cgctccggcc ctgtaggctc caagagcgct aagataagct gctccgctca
    12661 tgtttttaga gcctgctgcg atcagcacgc ggccaaaggt ccctttattt gaataggctt
    12721 tccgctccgg tacccggttt aaatcgcttt cctcgcaggc atacgccata gagatcggat
    12781 cctcctcttc cggctctgcc cttaaggcct cctcaggaaa accgatgtct gcaaccgtaa
    12841 catttcccgc atagtccctt cccggatata cggcagttcc aatcttctcc cagccaaagg
    12901 ctacggtttc atctgctttc agagcaatcc ccatcaccgc tcccgtaccg gaatggatcc
    12961 cggaaggaat atcgacagca atgcaccatg gctttgtttt tccattccct ttaagaatct
    13021 ctgctgcaag ctccatacag gctctgtatt ctccctggat ttcccggctt aatcccacac
    13081 cgaaaaggcc gtcgaaaaga atcccgcagt tttctgcttc ctctttcata agttcccatt
    13141 tttttacagg tatccccagc ttcctggcaa tggaaagctg gagctttaat tcttctgtcg
    13201 ccttttcctc tgaactggcc agaaaaataa ctgcaggaag tccttttaaa taaaccatcc
    13261 gggctgccgc cgctgcatcc gctccattat ttcccacacc gcacagcgcc cagaccggac
    13321 tgtgtgcggc aaatgctcgg gctgccttta atgcgcagtc tgcgaccgcc aaagctgccc
    13381 gctccattaa aaccagggaa ggaatcccga cctcatggat ggtgtaatga tccactgctt
    13441 tcatctttct acctgtcaga agggttctca tatcatgttc tcctctctgc ggacgtttcc
    13501 ttttcgccca ctgcaaatgc catggcaaaa tcctctgtat tggagataga cacatggatc
    13561 tgtgtcagcc ccatttcctc agatcgcttt ttagcgccgc cgtaaagatt gacatagggt
    13621 tttccaagct catcccgaag cacctcgata tctccgggca taaatccccg gaacccggtg
    13681 ccgaacatct ttgccacggc ctccttcaca gcaaaagagc cggccagctt agaggcagac
    13741 cccttcgctt gccggctttc cgtctccgta aatgtgcgag cgacaaaatg aggcctcctg
    13801 caggcctttt ccatgcgctt tatttcgatc agatcggttc caactcccag tatcatccta
    13861 cttgcttcct gtgcgttcgt ttttttcatc ccggtccagc atactgcata cccggtagga
    13921 cagccgcatc agactttcag acagatgcac attttccaca actacatcct ccggtacaac
    13981 aagatccgta tgggcgaaat tccagattgc cttgattcct gctgctacca gacgatccgc
    14041 aatctcggga gcctttgtct ttggtatggt aagagccgcg atctggatct cattctcttt
    14101 tataaagctt tccagatcgt ccacggaccg gatttcaatc ccgcggatca ccagaccgat
    14161 cagcttggga ttgatatcaa acatcccttt gatcacaaaa cctctccgtt caaaatttgt
    14221 ataattggcg attgcctgtc ccagatttcc ggctccgatg atgatcagat tgtgctgctg
    14281 gtcgattccc agaatctttg caatttccga gtacagatat tttacattat agccatagcc
    14341 ctgctgtccg aagccgccga aattattcag atcctggcgg atctgagaag ctgtaacctt
    14401 catgcggttg ctcagttcat tggaagagat acgctccact ccttcttcaa tcagctcccc
    14461 cagatagcgg taatagcggg gcagtctcga aataaccgct ttcgatattt ccttgcgttc
    14521 catcctgtgc caccctctcc ttatatgatt caatctaatg ctttctatta taatcgggcg
    14581 ttaaaaaaat gtcaaatcac tttcaggatg ttcttgaaaa aaatatgttc ctatattata
    14641 ctgattggtg catattatgc aaaaaacata gaatttatgc taaaaaagga gttttgaaat
    14701 gatcctgtca tgcagcaaca tcagccggtc ctttggagat aaccatatcc tcaaacgggt
    14761 ctcatttcat atagaggaac atgaaaaagc cgctgtcgta gggattaacg gcgcgggaaa
    14821 atccactctt ttaaagatca tcatcggtga tctggcacca gatgagggaa gtgtaacctg
    14881 ggccaagggc gcttccatcg gatatctggc ccagcatcag gatctggagg gcgctgagac
    14941 catttatgac gctcttttag aggtaaagcg gcctatcctt gaaatggagg agcgtctacg
    15001 aaaccttgaa cagtccatga aatctgcctc cggtgaagaa ctggaggcta tgctttccga
    15061 atacagccgc ttaaaccacg cgttcgagct ggaaaacggc tacgcctgcc gcagtgaaat
    15121 tacgggggta ttaaagggtc tgggattttc agaggatgaa ttttcaaagc ccatccaggc
    15181 gctctccggc ggccagaaaa cccgtgtatc cttaggcaag cttctcttaa ccaaacccga
    15241 tatcctgctt ctggacgagc ccaccaacca tctggatatg gagtctattg catggctgga
    15301 aacctattta aagggctatt caggaagcgt gatcatcgtg gcccacgacc ggtatttcct
    15361 ggaccgtgtg gttacaaagg tcatcgagct ggacaatggc accgccactg ttttttccgg
    15421 caattactcc gcctacagcg ataaaaaagc catgctcaga gacgcacaga tccgtgctta
    15481 cttaaatcag cagcaggaaa tccgccatca ggaagcggtc attgccaaat taaaatcatt
    15541 taaccgtgaa aaatccatcc gaagggcaga aagccgggaa aagatgctgg ataaaatcga
    15601 gcgtctggaa aagccagtgg aaatcaacga ttccatggat atccgtctgg aaccggacgt
    15661 agtcagcgga aatgatgtac tcaccgtcac agacttaagc aagtcctttg atactcagac
    15721 attatttaca catggcagct ttgaaatcaa acgtggggaa cggattgcgg ttatcggaaa
    15781 taacggtacc ggaaagacca cgcttcttaa gatcataaac ggcctgatcc ctgctgatgc
    15841 cggagaaatc cgcctgggcg ccaaggttca tatcggctac tacgatcagg agcatcaggt
    15901 cctccacatg gataaaaccc tgtttcagga aatccaggat acttatccca atatgaacaa
    15961 cactcagatc cgcaataccc ttgcttcttt cctctttacc ggtgatgatg tgtttaagct
    16021 gatcaaggat ttaagcggcg gcgaacgggg ccgcgtttca cttgcaaagc ttatgctttc
    16081 tgacgccaat ttcctgctcc ttgacgagcc caccaaccat ctggacatta cctcaaagga
    16141 gattctggaa agtgcattaa accgatatac aggaacggtg ctttacgtgt cccatgaccg
    16201 ctatttcatc aaccgcaccg ctacccggat tctggatctg accggacagt cctttataaa
    16261 ttatattggc aattatgatt attatctgga gaaaaaggag gatgtggagg ctgcattttt
    16321 tgcaggacga ggctctgagg ctccaaaatc agctttaggc cgtccggctg atgccggaac
    16381 gggagcttct tccggtaccg ctgcctcctc ttctgcttca gatacagggg ccaagcttga
    16441 ctggaaggct cagaaggaag agcaggccag gatccgcaaa cgtcagaatg aacttaaaaa
    16501 gaccgaagac gccatccacc agttagaaac aagagacagc gagatcaatg agctgctggc
    16561 gttggaagaa gtctataccg atgtgtccag actgatggag ctgaacaaag aaaaggattc
    16621 catcagtgaa aaactggaaa aactttacga attatgggag gcgctggcag aggaatgaaa
    16681 ttaaaacgta ttctggctct tcttggagtc cttctccttg cagggcttgt tctcctgttt
    16741 tttattctgg ctcttaccgg ggcttccaaa aatacactga tggctgtggc tttttcgatc
    16801 ctcttctttt ccgtcctgtt ttatgctatg gcgctgatgg caagggtatt aaagcatgga
    16861 gacgatccag gtcaggaaga ggatgaacga aaaaaaacat tgtaggttat tgataaagcg
    16921 ggaacctccc gggagtatgt cttcataggc tgcggtcatg aaaatgaccg cagtttcctt
    16981 ttttacgata ggatctgaat ttctgctgtc aacctctgca gttcattccg tgtaaatctt
    17041 actaattttt ttctctatga aatcccctta atggctgctt ccccatcgga tgaataccat
    17101 cttctgtttc acggacaggg tattgtcctg ccagctgctc taaatatttc tttcattttt
    17161 tcagaaggct accgtaatat tcttcctgtg tcaactatac atcccacaca tttactggat
    17221 ttctttttta ttttcattat cgggtttttc tcccattccc ggtttattgt ccatttctcc
    17281 gggtctttta ccattctccg ctggttccct tttcatgggc gctctcccgc ctgtgggaac
    17341 ttcgccgttc tcaggccccc taccgccttt aggagtctca ctgccaacgg gagcctttcc
    17401 gttttcagga atatcggtgc cgtaaacaag actgtccata gtaatcgttt tctcctccgt
    17461 accggctgtt aatgaatatc tttctccctg tctgatatcc gggctgctaa tgaggacaga
    17521 tgaatattct ttttctgctt tccaggataa aagctctgtg ccgtcactgt ttttcagggc
    17581 gatctgcgtt ccggcttcct gcggattcaa agtaaccatt attatcccct gggtagaaga
    17641 tggatcaaag ttttgtgcca taccggatgc gcctgatgaa gcaaaaattc ctcctgtgac
    17701 cactgctttt ccattataat ccaatgaact aatcccatct cctgacgcgt tgattctcag
    17761 actgccgcct gaaattgaaa tataggctcc ttccatagat acaaacggat cctgtccttt
    17821 ttccatactg cttccgtctc tgcctccggc agcattcaca ccgtcgtcag atgctgtaat
    17881 atcgatctgt cctcctgtaa tatcgatcgt caaaccttct attccttcgc agctttctga
    17941 aatcttaata ctgcctccgg aaatggcagc tccgtcatct gcatggattc catcatctct
    18001 ggaagacaga gtgtaatctc ctccggtgat aaaaacatat aaaatgttat tccccgtcgc
    18061 cagttatccc ttagcatagc cgggaaaatc cgtcaagggc atacgccgcc ggaggcggtg
    18121 ccatacggca cccttgacgg atttctcccg gttatgcttt tacacagtca agcgacgggg
    18181 atataaattc tccagagcca tcccccttcc cggggaaagg ggcggagggg aaaggcgaac
    18241 cttgctgata cccattctat ataagaagaa acggaggtta ttcaatgaaa cgacccttag
    18301 catatctgac tgccgcatgg agcggacagc cggaaatcga catggaactg gcggcgggct
    18361 actgccgcct tgcttatgag gcgggatttt ctcccatctg tccgctgatc taccttccgc
    18421 tgtttttaga cgacagcgta cccgaggaac acaaagccgg gattgacatg gcccgggata
    18481 tgctccgccg ttcccatacg ctgattgtct acggggaaac agtggatgaa gatgtgaaaa
    18541 acgatattgc agttgccgga aggcttggca ttgcggcaac gactttagag ggcgtcctgg
    18601 cagtaaaagg acatggcaga aatgcctgac attaagctgg gaagtctgtt tgacgggatc
    18661 ggggtctttc cccttgctgc ctcccggtgc ggaatccgct cggtgtgggc cagcgagatt
    18721 gagaaagccc cgatttccat taccaaacgg cacttgcctc ttttgcactt ctgttcggac
    18781 gatagccata gctacactct gcaaacagtg gttcatagat tggcactaac tgttgggtta
    18841 ttgcctgttg aagtgtacgg tctagcactg ttggtatgcc aagctttcgc acaccaccat
    18901 ctgatttggg aatctcaact cgtcttactg gagacggagt atactttcca cgataaatgc
    18961 ggtcggttat ctctcgttga tgttccttta aatatggaag agcctcctca atggtcatgc
    19021 cagcaattcc tggcgctccc ttgtttgcct taactctctt atacgctctg ttaaagttgt
    19081 ctttatacag tatcgcttcc aaaagtctcg gctgtgcact gtctctttct ttcca
//
LOCUS       DBMDUH010000035        19031 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_1745_length_19031_cov_6.146804, whole genome shotgun sequence.
ACCESSION   DBMDUH010000035 DBMDUH010000000
VERSION     DBMDUH010000035.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 19031)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 19031)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19031
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1745_length_19031_cov_6.146804"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..151)
                     /locus_tag="ACZPCG_04770"
     CDS             complement(<1..151)
                     /locus_tag="ACZPCG_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005350657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein"
                     /protein_id="HIR1284421.1"
                     /translation="MNDRIKLVANNKKAYHDYFIDDKYEAGIELFGTEVKSIRMGKCS
                     VKEAFV"
     gene            complement(232..2415)
                     /gene="rnr"
                     /locus_tag="ACZPCG_04775"
     CDS             complement(232..2415)
                     /gene="rnr"
                     /locus_tag="ACZPCG_04775"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HIR1284422.1"
                     /translation="MLTELMNDKAYVPMKAKELAMLLDIPKSRREELQEVLDKLVEDG
                     VIGVSKKGKYARSETFSVNGLFSGHSRGFGFVTVEGMERDVFIPEDKTGAALHGDRVQ
                     IVITSDGQKGRRPEGAVIRVLEHANREVVGYFQKSKNFGFVVPDNVKLSRDIFIPQGC
                     SQGAVTGHKVVVQLTDFGGPGRKPEGRITEILGHVNDPGTDILSLVRAYGLAESFPED
                     VMEELKAIPDELETEAVSEGKRFREDMPYYLDDLVSNDGWKEPYRGRLDLRGLQTVTI
                     DGEDAKDLDDAVTLGRTPEGNYILGVHIADVSHYVKENSALDREALRRGTSVYLVDRV
                     IPMLPHKLSNGICSLNEGTNRLALSCIMEIDGQGNVTDHRICETVICVDRRMSYTAVN
                     GILTGEMEGFQEEHAELVPLFKRMEELSGIVRERRRKRGAIDFDFPESKIFLDEKGRP
                     LEIRPYERNTATKIIEDFMLLANETVAEDFYWQSVPFLYRTHDTPNPEKMKQLSVFIN
                     NFGYFIRMQQGEIHPKELQKLLDKIEGTPEEPLLARLTLRSMKQAKYTTECSGHFGLA
                     ARYYTHFTSPIRRYPDLQIHRIIKEAIHGELKEKRQAHYEQLLPQVAVQTSALERRAE
                     EAERETEKLKKCEYMAKHIGETFEGVISGVSNWGFYVELPNTVEGMVHISELRDDYYI
                     FDESRYELTGELKKKTYKLGQPVRVVVSGTDRMLRTVDFVLDRDL"
     gene            complement(2528..2779)
                     /gene="secG"
                     /locus_tag="ACZPCG_04780"
     CDS             complement(2528..2779)
                     /gene="secG"
                     /locus_tag="ACZPCG_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HIR1284423.1"
                     /translation="MAVIQVILSIIYVLLGIAISIVILMQEGKSNGLGGAIGGMAESY
                     WSKNKGRSMEGALEHFTRYGAIVFMIITLILNIILKVQG"
     gene            complement(2900..3871)
                     /locus_tag="ACZPCG_04785"
     CDS             complement(2900..3871)
                     /locus_tag="ACZPCG_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284424.1"
                     /translation="MKAVNVHRVWKGIFTAAVLSCLLAVPASAKEERTPVGRITLNFS
                     ADIQAGDSEAYIDVSVEDGSCSVESADFVNEQEYWAGGVRPKVEIWLEADSDCYFKKS
                     GKSAFTFTGDDVKYVSSSTKYDREMMRLVVTLDKLDEEDEDLEVSELIWDEDNAIAHW
                     DQNGIAKKYKVRLCRGRGGSSTEDGVGSVHTVTENSFDFAEQIPKAGNYYFKVRAVDI
                     RDNLGDWEESPWFEVTEEDLSEWKGSWKQNERGWWYENRDGSWPKECWQMIDSDWYFF
                     DSEGYMKTGWIDWNGKSYFCSESGAMLVSCTTPDGYSVGEDGAKILP"
     gene            4309..5448
                     /locus_tag="ACZPCG_04790"
     CDS             4309..5448
                     /locus_tag="ACZPCG_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HIR1284425.1"
                     /translation="MKTVTGIYTSAIIYHTNNPKTSIEPYALAQIQMLCDNPAFEGSK
                     IRIMPDVHPGKAGTIGFTATVGKQILPQTVGIDIGCGITMARLSGKVRTDHFPKLDSL
                     IREQIPSGSQIRRTLHRYADHFHPEAFCCKNSINEVKAVLSLGSLGGGNHFIELDQDE
                     NGCFYVIIHSGSRCLGKNIFDHYMKKGQKYLKKQGLHVPYELTWLEGGLKEQYLNDLE
                     LTQQFAALNRKAILDELLRGMKWKADTVISCQHNYVDQTQNPPILRKGAISAQKDEPV
                     IIPIHMKDGVILGKGLGNPDWNCSAPHGAGRIMKRVDVGKHWTLSDYKKEMKGIYSPS
                     ISRRTLDEAPFAYRSLEEIRSAAADAVSIEKILRPVYNFKAGEEA"
     gene            5451..5627
                     /locus_tag="ACZPCG_04795"
     CDS             5451..5627
                     /locus_tag="ACZPCG_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015513788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284426.1"
                     /translation="MAKYREIPCKYYEAFGLCQKGRNACHKTYCQHCGKYVPRAKVRC
                     INKKKEFRFHSPIS"
     gene            complement(5609..6601)
                     /locus_tag="ACZPCG_04800"
     CDS             complement(5609..6601)
                     /locus_tag="ACZPCG_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR1284427.1"
                     /translation="MDQRRKDKKRKWFRPLKQGFLIAFSAGCLAACGQTASYQNAKGS
                     AAEYTPGQIMTVAATERNRYQNIYTGQLWSVAADGSGTTFESLLKGQMEQFLTELAAI
                     NLLAEEKGVELTNQEKTQVQSLTDTYFSSLTPEDLDYMKVSKDEIYDLYSKYYLADKT
                     VSQLTDEKALEVSDAEAKVIRILQIETDTEEKAETLWKKVNEPKADFASIAEKESGGS
                     QVEFQLEWSDTLNALEETAFSLEQDQISEIISWEGKFYIQKCISAYDEEATAARKLRL
                     LEEKKARAFDQIYTPFIQNHPIRLKNGIWEGVDFSQGEECTSDDFFQLYHEMGE"
     gene            complement(6628..7413)
                     /locus_tag="ACZPCG_04805"
     CDS             complement(6628..7413)
                     /locus_tag="ACZPCG_04805"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1284428.1"
                     /translation="MIKLIVSDVDGTLVEDGSADINPELFEVILKLREKGIQFAIASG
                     RPWSSVEHTFDPVKKKIFYISNNGSYIGCHGRSLYTYTIDKKLAERVIRSVRRHPELE
                     LVYACAGGDYVDSKNEDLVRWLVDGYKFKLNRVEDLLDLEEPPVKISIYKPDGIEEAT
                     RFVFDEFGTELKLACAGDMWMDCMALGVNKGNAVKTIQESLDIRPEETMAFGDQLNDL
                     EMLDQAYYSFAVANAREEVRRAARFQADSNVNDGVLKILKYLL"
     gene            complement(7406..8029)
                     /locus_tag="ACZPCG_04810"
     CDS             complement(7406..8029)
                     /locus_tag="ACZPCG_04810"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007709489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HIR1284429.1"
                     /translation="MKMTEYFKGHPVVLASGSPRRKELLEQIGIKARIRPSVQEEKSQ
                     AETPEELVMELAAVKAQDIAAGCESGTLVIGADTVVVLDEKILGKPQTPERAVEMIQK
                     LQGREHQVYTGVTVILCMENGELHGTSFAERTNVELWPMTEDEISTYAQSGEPLDKAG
                     AYGIQGSFAAYIKGIQGDYTNVVGLPLGRLVHEMNTLLTENGGTQDD"
     gene            complement(8170..8610)
                     /locus_tag="ACZPCG_04815"
     CDS             complement(8170..8610)
                     /locus_tag="ACZPCG_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HIR1284430.1"
                     /translation="MLGNQILVSAVVGWTVAQVLKTLIDCALNKSFNAERLVGSGGMP
                     SSHSATVCALTTAAALEYGAGSFEFAVSFVLAMVVMYDAIGVRRETGKQAKLLNSILM
                     ENPLKLNAEVLQEKLKEYVGHTPLQVLAGAILGIGLSLLLDSYY"
     gene            complement(8684..10126)
                     /gene="glgA"
                     /locus_tag="ACZPCG_04820"
     CDS             complement(8684..10126)
                     /gene="glgA"
                     /locus_tag="ACZPCG_04820"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007709485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - alpha-1,4-glucan
                     glucosyltransferase (UDP-glucose donor) activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HIR1284431.1"
                     /translation="MKKILFAASEAVPFIKTGGLADVVGSLPKCFDKDYFDVRVMIPK
                     YLCIKQEWRDRMQYVDHFYMDYLGQSRYVGILQYVHEGITFYFIDNESYFNGEKPYGD
                     WYWDLEKFCFFCRAALSALPVIGFQPDVVHCHDWQTGLIPVLLKDTFRGGDFFRNMKS
                     VITIHNLKFQGVWDVKTIKRFTELPDYYFAPDKLEAYKDGNLLKGGIVYADAITTVSN
                     TYAEEIKMPFYGEGLDGLMRARANSLRGIVNGIDYDDFDPETDPFIAQKYNAKNFRKE
                     KAKNKKALQQELGLPVDEKKFMVGIVSRLTDQKGLDLIQCVMDELCSDDMQLVVLGTG
                     DERYENMFRHYDWKYHDRVSAQIYYSEAMSHKIYAACDAFLMPSLFEPCGLSQLMALR
                     YGTVPIVRETGGLKDTVQPYNEYESTGTGFSFANYNAHEMLGSIRYAKYVYYSRRREW
                     NKIIDRAMAADFSWKTSAAKYQELYDWLIG"
     gene            complement(10227..11057)
                     /gene="spo0A"
                     /locus_tag="ACZPCG_04825"
     CDS             complement(10227..11057)
                     /gene="spo0A"
                     /locus_tag="ACZPCG_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA];
                     GO_process: GO:0051606 - detection of stimulus [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HIR1284432.1"
                     /translation="MEKQEQLNVVIVDDNPMVLSTLDEMINAEDGLTVIGKADNGEDA
                     IDMIKDTTPDIVLLDMVMPKVDGITIVEKFNHRDTFRKHPVFIMLSAAGSELVTEEAF
                     QAGVNYYLMKPFDRDILMRKIRHAGHLPRRITAGKMMEVPRPAVMEEPAVTKEEYMEE
                     HLEADITKMLHELGIPAHIKGYQYLRDAIIMVVKDQEMMGSVTKILYPAIAKKNQTTA
                     SRVERAIRHAIEVAWGRGKMETIDELFGYTISTGKGKPTNSEFIALISDKIFLEYKKI
                     "
     gene            complement(11272..12558)
                     /gene="spoIVB"
                     /locus_tag="ACZPCG_04830"
     CDS             complement(11272..12558)
                     /gene="spoIVB"
                     /locus_tag="ACZPCG_04830"
                     /EC_number="3.4.21.116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006779127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase"
                     /protein_id="HIR1284433.1"
                     /translation="MRRTGGFYRKLALGFWLSAIAGGAFVWHLAYRAVPDQISMLENQ
                     KEEFSFSLPWRATLSSESAEVSLGNESNIPGDEITINSSEPFHVFAGKKGSYQMNLKL
                     LGLIKLKEIQVDVVDTRYAIPCGSPIGIYLKSQGVMVVGTGRITREDGRETEPAFGKL
                     KSGDYIEAFNGTPLSTKEDLVREVGKWNGGEASLTIRRRGENMEVALSPVKGEDGSYK
                     LGAWVRDDTQGIGTITYVDPNGHFGALGHGISDSDTGELVEIETGALYTAKIMGVEKG
                     EAGKPGLLSGVIYYGPQSCRGEITANTEDGIFGMVDQQLKKQIQGEPMEVARCQEVKP
                     GPAVIRTAVSGEVKDYTVEIQKVDQSPSKRNKAMVLKVTDPELLSLTGGIVQGLSGTP
                     ILQNGKLAGAVTHVFVQDPSRGYGILADDMMKYGEE"
     gene            complement(12630..14306)
                     /gene="recN"
                     /locus_tag="ACZPCG_04835"
     CDS             complement(12630..14306)
                     /gene="recN"
                     /locus_tag="ACZPCG_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006771991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HIR1284434.1"
                     /translation="MLLELHVKNLALIERADVEFGEGLNILTGETGAGKSIIIGSVSM
                     ALGGKASRDSIRHGADYAYIELVFSVKGEDRLRALRELDTEPTEDGLVIISRKITPSR
                     SISRINDETVTAARLRQITGLLLDIHGQHEHQSLLYKSKHLEILDAYVKAQTQPAKKK
                     MEEEYKNYCILKKRLSSFEMDHDARLREADFLRFEIQEIEEGNIKEGEEEELTARYRR
                     FSHARRIAENLNEAYDSLQADAVTRALRSVEQVTEYDEALQSIRDQLYDAEAILSDAG
                     REISAYISSMEFDEESWRQTEERLDQIHSLQNKYGASVLAIQASLEEKRKRLEELENY
                     DAFREQTEKKLKISTDHLCRLCTDLSEIRKKASVELVKRIRSGLLDLNFLDVEFDMEF
                     ETLDHFTPDGWDEVQFLISTNPGQPMRPLKDVASGGELSRIMLAIKTVLADSDEIPTL
                     IFDEIDTGISGRTAQKVSEKLSYIAGSHQVICITHLPQIAAMADIHFEILKSASEGST
                     ATTIERLGREKSIRELARLLGGAQITEAVLKNAHEMKDLADQTKKPWMER"
     gene            complement(14312..14764)
                     /gene="argR"
                     /locus_tag="ACZPCG_04840"
     CDS             complement(14312..14764)
                     /gene="argR"
                     /locus_tag="ACZPCG_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HIR1284435.1"
                     /translation="MKVERHSKIVELIGKYEIETQEELAEKLNQSGFNVTQATVSRDI
                     RELKLTKIQGENGRQRYMVLESPRGTSAIKYIRILKDGFMSMDMAQNILVIKTVAGMA
                     MAVAAALDAIHFQEVVGCIAGDDTIMCAVRSVDDTIIVMEKIKKMVED"
     gene            complement(14819..15658)
                     /locus_tag="ACZPCG_04845"
     CDS             complement(14819..15658)
                     /locus_tag="ACZPCG_04845"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006741 - NADP+ biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD+ metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HIR1284436.1"
                     /translation="MKRFFLIANLAKEFVPETRAYIRECLEKRGAECRFLTLAQHNKY
                     GKHTPSELVPEDTECVITIGGDGTLIQAARDLAGRNIPLLGVNRGHLGFLNQISSQDE
                     IEEAIASLIENRFELERRMMIRGTVFHQGKPVFQDIALNEIAVTSRVPLRVLRYQVSV
                     NDEYLNHYSADGVLVATPTGSTAYNLSAGGPVMAPAGRMMALTPVCPHSLNARSIVLA
                     AEDRIQIGVLSSGRVSFDGDSWADLETGDCLIAERSALETVMIRRKKQSFMQNLSDHL
                     SGI"
     gene            complement(15679..16506)
                     /locus_tag="ACZPCG_04850"
     CDS             complement(15679..16506)
                     /locus_tag="ACZPCG_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015573509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HIR1284437.1"
                     /translation="MKERLDVLLVKRNLAESREKAKALIMSGIVYVNGQKEDKAGTTF
                     EETAPIEVRGSTLKYVSRGGLKLEKAMDRFGVRLDGKICMDVGASTGGFTDCMLQNGA
                     VKVYSVDVGHGQLAWKLRNDERVVCMEKTNIRYVAPEDIADPIGFASIDVSFISLTKV
                     LGPVKALLEDNGQIVCLIKPQFEAGREKVGKKGVVREPAVHLEVIDMVIDYALSIGFE
                     ALNLEFSPIKGPEGNIEYLLYLQKHPEDQEAEYAVRREAAADPKAVVAEAHQTLDRS"
     gene            complement(16525..18438)
                     /gene="dxs"
                     /locus_tag="ACZPCG_04855"
     CDS             complement(16525..18438)
                     /gene="dxs"
                     /locus_tag="ACZPCG_04855"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HIR1284438.1"
                     /translation="MILEQIKGPEELKQLSSRELAILAEEIRTFLIEKISRTGGHLAS
                     NLGVVELTIALFKTFDLPQDKIIWDVGHQSYTHKILSGRMAEFDELRQYGGISGFPKR
                     KESPYDSFDTGHSSTSISAGLGIAQGRDLLGEDYRVVSVIGDGALTGGMAYEALNNAA
                     RMKKNFIIILNDNEMSISENVGGMSRYLSGLRTGTGYNDLKKNIVEALERVPLVGNAV
                     IDKLKRTKDSIKQLFIPGMLFENMGITYLGPVDGHNIPVLCKVLKEAKKLDHAVLIHV
                     ITKKGKGYKPAEKNPSIFHGVGPFDIATGKENGKKKEPSYTEVFSRKLCQLGETHPKL
                     VAVTAAMPDGTGLAAFGKKFPDRFFDVGIAEAHAVTSAAGMAAAGLRPVVAIYSSFLQ
                     RGYDQVLHDVCIQNLPVLFAVDRAGLVGSDGETHQGIFDYSYLTSIPNMTVMAPKNLW
                     ELRAMMEFAMDYNAPLAIRYPRGTAYRGLREYRQPIVYGKGELLCEESDIALLAVGSM
                     VSTAEHIREKLKEEGYSCSLANGRFVKPFDKELTERLAKNHRLLVTLEENVLQGGYGL
                     AVTAYIHSHYPEVKVVNIALPDAYVEHGNVSLLRKGLGIDSDSIIRRLKEEGWLSGIH
                     REENGEERQRPSE"
     gene            complement(18468..>19031)
                     /locus_tag="ACZPCG_04860"
     CDS             complement(18468..>19031)
                     /locus_tag="ACZPCG_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR1284439.1"
                     /translation="LAGDALMMYAFETASKAFDAAEDADALLRAGKAMGILAAKTGVY
                     GMIGGQTVDVELAGGLVPKDKLDFIYRLKTGALLQAAMLIGAVLGGAADEDCKIVEDL
                     AGKVGLAFQIQDDILDVTGDASVTGKAAGSDEKNQKTTYVTLEGLSKARKEVEALSAA
                     AIGDLDKLPGNNEFLEQLIRLLVNRER"
BASE COUNT         4616 a         5347 c         3710 g         5358 t
ORIGIN      
        1 ttacaaaagc ttccttcaca ctgcactttc ccatgcggat cgatttgacc tctgtaccga
       61 aaagctcaat tccggcttca tatttatcat caataaaata atcatggtag gcttttttat
      121 tatttgctac cagcttaata cgatcgttca ccttttacct ccctctgtct ctcttttgct
      181 cagacagatg ttattatcat accacaggcc cacagtgaag taaagaaatt gttacagatc
      241 tcgatccagc acaaaatcta ccgttcgaag catccggtcc gtccctgata cgaccacgcg
      301 caccggctgt cccagcttgt atgttttctt tttcagttct cctgtaagct catatcggct
      361 ctcatcaaaa atgtagtaat catcccggag ctcgctgata tgaaccattc cctccaccgt
      421 gttgggaagc tccacataaa agccccagtt ggaaacgccg gagatcaccc cctcaaaggt
      481 ttctcctata tgctttgcca tatactcgca tttctttaat ttctccgttt cccgctctgc
      541 ttcctctgca cggcgctcta acgcagatgt ctggactgcc acctgaggaa gaagctgctc
      601 ataatgcgcc tgtcttttct cctttaactc tccatggatc gcttccttta taatgcggtg
      661 gatctgaaga tccggatacc tgcggatggg agaggtaaaa tgggtatagt agcgggccgc
      721 cagtccaaaa tggcccgaac attccgttgt gtattttgcc tgtttcatgg aacgcagcgt
      781 cagccttgct aaaagcggtt cctccggtgt tccttcaatt ttgtccaaaa gtttctgaag
      841 ctcctttgga tggatttctc cttgctgcat acggataaaa tagccgaaat tattgataaa
      901 aacactcagc tgcttcattt tttccggatt cggcgtatca tgcgttcgat aaagaaaggg
      961 tacggactgc cagtaaaaat cctctgccac cgtttcattt gccaacagca tgaagtcctc
     1021 tatgatcttt gtagctgtat tgcgctcata gggacgaatc tccagagggc gtcctttttc
     1081 atccagaaaa attttactct cagggaaatc aaagtcaata gcccctcttt tcctgcgccg
     1141 ttctctcaca attcccgaaa gctcttccat ccgtttaaaa agcggcacaa gctctgcatg
     1201 ttcctcctga aacccttcca tttctccagt caaaattccg ttcaccgcag tatagctcat
     1261 ccgcctgtct acgcagatta ctgtttcgca gattctgtgg tctgtcacat tcccctggcc
     1321 gtctatctcc atgatacagc ttaacgccag acggttcgtt ccctcattta aggagcagat
     1381 tccattagaa agcttgtgcg gcagcatagg gatcacgcga tccaccagat acacgctggt
     1441 tcctctccga agcgcctccc gatccagagc gctgttctcc tttacatagt ggcttacatc
     1501 cgcaatgtgg actcccaaaa tataattacc ctccggtgtc cgtccaagcg tcaccgcatc
     1561 gtccagatcc ttggcgtctt ccccgtcaat ggtcacagtc tgaagcccac gaagatccaa
     1621 ccgtcctctg taaggctcct tccacccgtc attagagacc agatcatcca aataatacgg
     1681 catatcctca cgaaagcgtt ttccttcaga cactgcttct gtctccagct catccggtat
     1741 cgcttttaat tcctccatca catcctcagg aaatgattct gcaagcccat aggcccgcac
     1801 cagggataaa atatccgtgc cgggatcatt gacatgtccc aggatctctg taatccgtcc
     1861 ctccggcttt cttcccggac cgccaaaatc tgtaagctgc accactacct tatggcccgt
     1921 tacagccccc tggctgcagc cctgagggat aaatatatcc ctggaaagct tcacattatc
     1981 aggcacaaca aagccaaaat ttttactttt ctgaaaatat cctacaacct cacggtttgc
     2041 gtgctccagc acccggatca ctgccccttc cggacggcgt cctttctgtc catcggatgt
     2101 gatcacgatc tgcacccgat ctccatgcag ggcagccccg gtcttgtcct ctggaataaa
     2161 tacatccctc tccattccct caactgtaac aaatccgaat cctctggaat ggccgctaaa
     2221 aaggccgttc acagaaaaag tctctgatct ggcatatttt cccttctttg agactccgat
     2281 cacaccatcc tccaccagct tatcaaggac ctcctgaagc tcctctcttc tggattttgg
     2341 aatatcaagc agcatggcaa gctcttttgc cttcataggc acatatgctt tgtcattcat
     2401 cagctctgtc agcattttct ttctctgttc caattgttct ttttccatgt atcctctttt
     2461 ctgtctattc atttaaaaaa acacccttca atgcgataca tgaaagggtg tttttgtgtc
     2521 cgtattatta tccctgaacc tttaaaataa tattcaggat cagtgtgatg atcataaata
     2581 caatagcgcc gtatcttgta aagtgctcta atgcaccctc catggaacgg cctttatttt
     2641 tgctccaata gctctcagcc atgccgccga tagcgccgcc cagtccattg gacttaccct
     2701 cctgcatcag aattacgatt gaaatagcga tgccaaggag tacatatatg atcgataaaa
     2761 taacctgaat cactgccatg acctgtacct ctttccgttt ccttatagtc aaacaaaatt
     2821 aatatatcat actttttctg taaactcaag gcttttcttg cggttttacc cgaaaagacc
     2881 gccttatatc ccgaaaaagc tatggaagga tcttagcgcc gtcttctcct accgaatatc
     2941 catccggcgt tgtacaagat accagcatag ctcctgattc gctgcagaaa taggattttc
     3001 cgttccagtc aatccatcct gttttcatat agccttcact gtcaaaaaag taccagtctg
     3061 aatcgatcat ctgccagcac tccttcggcc aggagccgtc cctgttttca taccaccaac
     3121 cgcgctcatt ctgcttccag cttcccttcc actcagataa atcctcctct gtcacctcaa
     3181 accatggaga ttcttcccaa tcgcccagat tgtccctgat atcaacggct cttactttaa
     3241 aataataatt tcctgccttg ggaatctgct ctgcaaaatc aaagctgttt tctgtcaccg
     3301 tatgaaccga gcccacgcca tcctccgtgg agcttcctcc ccggccccgg cacagacgca
     3361 ccttatattt ttttgcgatc ccattctgat cccagtgagc aatggcattg tcctcatccc
     3421 agataagttc tgatacctcc agatcctcat cctcttcatc cagcttgtca agcgttacca
     3481 ccagacgcat catttcccta tcatacttgg tagagcttga aacatacttc acatcatccc
     3541 ccgtaaaggt aaacgcactt tttcctgatt ttttaaaata acagtcgctg tctgcctcca
     3601 gccatatttc cactttgggg cgaacgcctc ctgcccagta ttcctgctca ttgacaaaat
     3661 ctgcgctttc cacggagcag cttccatcct ctaccgatac atcgatgtac gcctctgagt
     3721 ctcccgcctg aatatccgcg ctgaaattca gcgtaatgcg gcctactggc gtcctctcct
     3781 ctttcgccga tgccggaaca gcaagaagac aggataatac tgctgccgta aaaatccctt
     3841 tccacactct atgcacgttt actgctttca ttgtgttttc ccctttccat ttcttatgta
     3901 tttttgcttt tctaagtata tcataatcca ctcatttaag ctaccttaac ccatttcatc
     3961 aaaaaccgaa gagttctttc aatcataacg catttttgtt ttgccatttt ctcatattga
     4021 caaatcctat ctttcccgcg tataatgagg ccataaaaca ggagcacgac tgtttgcagc
     4081 tatcaacccg gacgaaagga ggatttacta tgaatgcagc tataaacaac gatacagcag
     4141 tcaaatttct cagaggttct aaactccata tctgattgga tatttctgta ttcacaggca
     4201 aagtccattc caaatatttc cagtttcccg cctctgaacc taaccgttta tgaggtgttt
     4261 ttttgcgccc tttttcaggc agaacaccct tacagagaaa ggaatacaat gaaaacagta
     4321 actggaatct atacatccgc tatcatttat cacacaaata accccaaaac ctccattgag
     4381 ccatatgctc tggcccagat ccagatgctc tgtgacaatc cagcctttga aggcagcaag
     4441 atccgcatca tgccggatgt ccatcccgga aaggcaggca ccatcggttt tactgccact
     4501 gtcggaaaac agatcttgcc acaaacggta ggaattgata tcggctgcgg aattaccatg
     4561 gccagactgt ccggcaaagt ccgaacggat cattttccca agctcgactc actcatccgg
     4621 gaacagattc cctctggctc acaaatccgc agaacactcc accgatacgc agatcacttc
     4681 catccggaag ccttctgctg caaaaacagc atcaatgagg taaaagccgt tttaagtctg
     4741 ggaagtctgg gcggaggaaa ccattttatt gaactggatc aggatgaaaa tggctgtttt
     4801 tacgttatca ttcattctgg aagccgctgt ctcggaaaga atattttcga tcattatatg
     4861 aaaaaaggcc agaaatactt aaaaaaacag ggccttcacg ttccatatga gctcacatgg
     4921 ctggaaggcg gtttaaaaga gcagtattta aatgatcttg aactgacaca gcaatttgcc
     4981 gcactgaacc ggaaagctat tctggatgaa cttctccgcg gaatgaagtg gaaagcggat
     5041 acggtcattt cctgccagca taactatgtg gatcagaccc aaaatccccc catcctgcgc
     5101 aaaggcgcca tttcggccca aaaagatgaa cctgttatta ttcccattca tatgaaagac
     5161 ggggttattc tcggaaaagg tctgggaaat cctgactgga actgttccgc ccctcacggc
     5221 gcagggcgga tcatgaaaag agtagatgtg ggaaaacact ggactctttc tgattacaaa
     5281 aaagaaatga aagggatcta ttcccccagc atctccagga ggacattaga tgaagccccc
     5341 tttgcctacc ggtctctgga ggaaatccgt tcggcggcgg ctgatgccgt atccatagaa
     5401 aaaatcctgc gcccggtata caacttcaaa gcaggagagg aggcgtaaaa atggctaagt
     5461 acagagaaat cccctgtaag tattatgaag cgtttggcct ctgccaaaaa gggcggaacg
     5521 cctgccacaa aacctactgc cagcactgcg gcaaatatgt tccccgggca aaggttcgct
     5581 gtatcaacaa aaagaaggaa tttcgctttc attcccccat ctcatgatac agctgaaaaa
     5641 aatcatcgga ggtgcactct tctccctgtg aaaagtccac gccctcccaa atcccgtttt
     5701 tcaggcggat cggatggttc tgaataaacg gcgtataaat ctggtcaaag gctctggctt
     5761 ttttttcttc caaaagccgg agctttctgg cagctgtagc ttcctcatca taggccgata
     5821 tgcatttttg aatataaaat ttcccttccc aggaaataat ttcgctgatc tgatcctgtt
     5881 ccagagaaaa agccgtctcc tccaaagcat ttaatgtatc gctccactcc agctgaaact
     5941 cgacctggct gccgcccgac tctttttcag cgatagaagc aaaatcagct tttggctcat
     6001 tgaccttttt ccacagcgtc tccgcctttt cttctgtatc tgtttcaatc tgcagaatcc
     6061 ggatcacttt tgcctctgcg tcacttacct ccagcgcttt ttcatctgtc agctgagata
     6121 cggttttatc cgccagataa tatttgctgt ataaatcgta gatttcatcc ttggatacct
     6181 tcatataatc cagatcctcc ggtgtaaggg atgaaaaata ggtatccgtc agactctgca
     6241 cctgcgtctt ttcctgattg gtaagctcaa cccctttttc ttctgccaaa agatttatgg
     6301 ctgccagttc tgtcaaaaac tgctccatct ggccttttaa aagggattca aaggtggttc
     6361 ccgatccatc agccgcaaca gaccagagct gtccggtgta gatgttctga tagcggttgc
     6421 gctccgtcgc cgcaacagtc atgatctgcc ccggcgtgta ctctgctgca gaaccctttg
     6481 cgttctgata ggatgccgtc tgtccgcagg ccgccagaca tcccgctgaa aacgcgatca
     6541 gaaatccctg ctttaaaggc cggaaccatt ttcttttttt atccttcctt ctctggtcca
     6601 atgaagtctc ctttccagtc gtacattcta cagaagatat ttaagaattt tcaaaactcc
     6661 gtcatttaca ttgctatccg cctgaaatct ggcagcccgg cgcacttcct ctctggcatt
     6721 tgccacagca aagctgtagt atgcctgatc aagcatctca agatcattga gctgatcgcc
     6781 aaacgccatt gtttcttccg ggcggatatc cagactctcc tgaatggttt ttacagcatt
     6841 tcccttattt acccccaaag ccatgcagtc catccacata tctccggcac aggcaagctt
     6901 aagctctgtt ccaaattcat caaacacaaa acgggtggcc tcctcgatgc cgtccggctt
     6961 gtaaatagat atttttacag ggggctcctc aagatctaat aaatcttcca cccggttcag
     7021 cttaaattta tacccatcaa caagccagcg cacaaggtct tcattttttg aatccacata
     7081 atctcctccg gcacaggcat aaacaagttc aagctccgga tgacggcgga cggagcggat
     7141 cacccgttct gccagtttct tatcaatcgt ataggtataa agactgcggc catggcagcc
     7201 aatataggaa ccgttattgg aaatataaaa aatctttttc tttaccgggt caaaggtatg
     7261 ctctacactg gaccagggcc gtccgctggc tatggcaaac tgaatcccct tttctctcag
     7321 tttcaggatc acctcaaaca gttccggatt gatatcggca gatccgtcct ctaccagtgt
     7381 tccatctaca tcggatacta taagtttaat catcctgtgt tcctccattt tctgtcaaaa
     7441 gtgtgttcat ctcatggacc agacgcccta aaggcagtcc taccacattg gtataatctc
     7501 cctgtatccc ctttatatag gccgcaaaac tgccctgtat cccatatgcg cctgccttat
     7561 caagcggctc cccgctctgt gcatatgtag aaatttcatc ctctgtcatg ggccagagtt
     7621 ctacatttgt gcgctccgca aaggatgttc cgtgcagttc tccattctcc atacacagga
     7681 ttaccgtaac accggtataa acctgatgtt cccggccctg aagcttctgt atcatttcga
     7741 cagccctctc cggggtctga ggctttccaa gaattttctc atccaggaca accactgtat
     7801 cggctccaat gactaaggtt ccggactcac agcctgcggc aatatcctgc gcctttacag
     7861 cggccagttc catcaccagc tcctccggcg tctcagcctg ggacttttcc tcctgcacgc
     7921 tgggcctgat ccgggctttg atcccaatct gttccaaaag ttcctttctt ctgggagaac
     7981 cggaagctaa aaccaccgga tgacccttaa aatattctgt cattttcatt tctattctcc
     8041 tgctcttatc tgtaaatatg ggaatatgcc tgtacgtggg aaaataccta cgacgcaaaa
     8101 ggggccatgg caaaagccac ggtccgcatc tgctggttca tatgaatgta aggggctgct
     8161 gatgatttct caataatagg aatccagaag cagagacagg ccaattccca aaatcgctcc
     8221 ggccagaacc tgaaggggcg tatggcccac atactcctta agtttctcct gaagaacctc
     8281 cgcattgagc ttcagaggat tttccatcaa aatggaattt aaaagctttg cctgttttcc
     8341 cgtctctctg cgcactccga tggcatcata catgacgacc atggccagaa caaaggatac
     8401 ggcaaattca aaggagcctg cgccgtattc cagcgccgca gccgtcgtca atgcacagac
     8461 cgtagctgaa tgggagcttg gcataccgcc cgatcccacc agacgttccg cattaaagct
     8521 tttgtttaaa gcgcagtcga tgagcgtctt taaaacctgg gctactgtcc agcctactac
     8581 ggcacttaca aggatctgat tgccaagcat atcgttccaa acattcatag tgtagtttct
     8641 ttcccctttc ttcgggtatt tatctgttta aatgcagtgt ctctcagccg atcagccagt
     8701 cataaagctc ctgatattta gctgctgagg tcttccatga aaagtctgcc gccatggcac
     8761 ggtcgattat cttgttccac tcccggcgtc tgctgtaata tacatacttg gcatagcgta
     8821 tgctgcctag catctcatgc gcattgtaat tggcaaagga aaatcccgtt ccagtactct
     8881 catattcatt atatggctga accgtatcct tcaaaccgcc ggtttcccgg acaatgggta
     8941 ccgttccgta gcgcagggcc ataagctggc ttaaaccgca gggttcaaac agggacggca
     9001 tcaggaatgc gtcacaggct gcataaatct tgtgagacat ggcctcagaa taatagatct
     9061 gggcggaaac cctgtcgtga tacttccagt catagtgacg gaacatattt tcatagcgtt
     9121 catcacccgt tccaagaacc accagctgca tgtcgtcact gcaaagctca tccatcacac
     9181 actggatcag atccaggccc ttctggtctg taagacggga tacaataccc accataaact
     9241 tcttctcatc cacaggaagt cccagctcct gctggagcgc ctttttgttt ttcgcctttt
     9301 ccttgcggaa attctttgca ttatatttct gggcgataaa aggatcggtc tccggatcaa
     9361 aatcatcata atcaattccg tttacgattc cccgaagact gttggccctg gcccgcatca
     9421 ggccatccag gccttctccg taaaacggca tcttgatctc ctctgcgtat gtattgctta
     9481 ccgtggtaat ggcatccgca tatacaatac cgccctttaa cagattgccg tccttatagg
     9541 cctccagctt atctggagca aaatagtaat ctggaagctc tgtaaaacgc ttgatggtct
     9601 ttacatccca caccccctgg aactttaagt tgtggatggt gataacagac ttcatattgc
     9661 ggaagaaatc acctccccgg aaagtatcct ttaatagtac aggaatcagg cctgtctgcc
     9721 agtcatggca gtggaccaca tccggctgga agccgatcac aggcagggca gaaagagcag
     9781 ccctgcagaa aaagcagaac ttttccagat cccagtacca atcgccatat ggcttttctc
     9841 cgttaaaata gctttcatta tcaataaaat aaaatgtgat cccctcatgg acatactgta
     9901 aaattcctac ataacggctc tgtcccagat aatccatgta aaaatgatcc acatactgca
     9961 tcctgtctct ccactcctgc ttgatgcata gatatttggg aatcatgact cttacgtcaa
    10021 aataatcctt atcaaaacat tttggcagag aacctactac atctgccagt cccccggttt
    10081 taataaatgg tactgcttca gaagctgcaa ataaaatctt cttcatccaa acctccctct
    10141 cctgacgtat ccaggcggcg ttttgcctta ccatatagta tagcgtactt tttcaaaatt
    10201 aggaagctgt tttcttagga tttgtcttaa atttttttgt attccaggaa tattttatca
    10261 gaaatcagag caatgaattc agaattggta ggctttcctt ttcccgtact gatggtgtat
    10321 ccaaacagct catcaatcgt ttccatttta cctcttcccc aggccacttc aatcgcatgg
    10381 cggatcgcgc gctctacgcg gctggctgta gtctgatttt tcttggcaat cgctggataa
    10441 agaatttttg taacagaacc catcatttcc tgatccttta caaccataat gatcgcatct
    10501 cttaaatact ggtagccctt gatgtgggcg ggaattccca gttcatgaag catctttgtg
    10561 atatctgctt ccagatgttc ttccatatac tcttcctttg taaccgcagg ctcttccatc
    10621 acagccggcc ttggcacttc catcatcttt cctgccgtga tccggcgcgg aagatggcct
    10681 gcgtgacgga tcttcctcat gaggatatcc cggtcaaagg gcttcatcag ataataattt
    10741 actccggcct gaaacgcttc ctccgtaacc agctcacttc cggcagcact gagcatgata
    10801 aaaacaggat gcttgcgaaa cgtgtctctg tgattgaatt tctcaacaat tgtaatcccg
    10861 tctacttttg gcataaccat atccagaagc acaatatctg gtgtagtgtc ctttatcata
    10921 tcaatggcat cctctccatt gtcagctttt ccaatgaccg tcagcccgtc ctcggcattg
    10981 atcatctcat ccaaagtact taataccatg ggattatcat ccacaattac cacgtttaat
    11041 tgttcctgct tttccatttt tcctccttat tacatcagga ccgggagtat tatccccccc
    11101 ttgtccaaac tagcacaaag gagccgccca tttacggccc ctttgtcttt aaaagtaaca
    11161 ttttatatta taccgttact tattgtcatt tgacaatata tggtaatcgc aaatttagac
    11221 acaaaatttc gaccattttt agtgtttttc gactatttgt aacccatctt ttcattcttc
    11281 cccatatttc atcatatcat ctgccagtat tccatagcct cttgatggat cctggacaaa
    11341 cacatgtgtt acagctccgg ccagttttcc attctgaaga atgggagtac cggaaagtcc
    11401 ctggacaata ccgcctgtaa gcgaaagaag ctctggatct gttaccttaa gaaccattgc
    11461 cttatttcgt ttggacgggc tttgatccac cttctgtatt tctactgtat agtcctttac
    11521 ttcacccgaa acagccgttc ggatgactgc cggccccggc tttacctcct gacatctggc
    11581 gacctccatc ggttctccct ggatctgctt tttaagctgc tgatcgacca ttccaaaaat
    11641 accgtcctcc gtattagcgg taatctctcc cctgcaggac tgcggaccat agtagatcac
    11701 ccccgaaaga agccccggct ttcccgcctc tcccttttcc actcccataa tcttagctgt
    11761 gtagagagcg cctgtctcta tttccaccag ttctcccgta tcactgtcgc ttatcccatg
    11821 gcccagggct ccgaaatgtc cgttgggatc cacataagtg atagtaccga tcccctgggt
    11881 atcatccctg acccaggccc ccagcttata gcttccgtcc tctcccttta ccggagacag
    11941 ggctacctcc atgttttctc ctctgcgccg tatggtaaga cttgcttctc ccccgttcca
    12001 cttccccact tccctgacca gatcctcctt ggttgacagc ggcgttccgt taaacgcttc
    12061 aatgtaatcc cctgatttta atttgccgaa agccggctcg gtctcccggc cgtcttccct
    12121 ggtaatccgt ccggttccta ccaccatgac tccctgagat ttcagataga ttcctatggg
    12181 acttccgcag ggtatggcat acctggtatc cactacatcc acctgtattt ctttcagctt
    12241 gatcagcccc agaagcttca gattcatctg ataacttccc ttttttcccg caaaaacatg
    12301 aaatggctcg ctgctgttta tagtgatctc atcccccgga atattgcttt catttcccag
    12361 ggatacctct gcgctttcgc tggaaagcgt agcccgccag ggcagagaaa atgaaaattc
    12421 ctctttctgg ttttcaagca tactgatctg atcgggtact gcccggtatg caagatgcca
    12481 cacaaaggcc cctccggcta tggctgacag ccagaacccc agggccagct tccggtaaaa
    12541 tccccctgtt ctcctcatca tatgccctgc ctccttttct aatgatcctg tcattagtat
    12601 gagtctgcat ggcatatttc aatctggttt tatctttcca tccatggctt ttttgtctgg
    12661 tctgccaaat ccttcatttc gtgtgcattt ttaagaactg cctctgtgat ctgcgcgccg
    12721 cctaacaatc tggcaagctc tcttatgctt ttctcccgtc caagacgttc aatggtggta
    12781 gcagtggaac cctcactggc cgatttaagt atttcaaaat ggatatccgc catagctgcg
    12841 atctgaggca ggtgggtgat acagatcacc tggtggctgc ctgcaatata agaaagcttt
    12901 tctgatactt tctgggctgt gcgtccgctg atgcctgtat caatttcatc aaaaataagc
    12961 gtggggatct catctgaatc cgccagcacc gtcttgatcg ccagcataat acgagagagc
    13021 tcgcctccgg aagccacatc ctttaaagga cgcataggct gtcccggatt ggtggaaata
    13081 agaaactgaa cctcatccca gccatccggc gtaaaatgat ccagcgtttc aaattccata
    13141 tcaaattcca catccaggaa atttaaatca agaagcccgc tccggatccg ttttacaagc
    13201 tccaccgatg cttttttacg tatttctgat aagtctgtac agagtctgca aagatgatcc
    13261 gtggaaattt tcagcttctt ttccgtctgt tccctaaatg catcataatt ttccagttct
    13321 tccaggcgtt tccttttttc ttccagactg gcctggattg ccaaaaccga agctccgtat
    13381 ttgttttgga ggctgtggat ctgatccaga cgctcctccg tctgtctcca gctttcctca
    13441 tcaaactcca tactgcttat ataagctgaa atttcccttc cggcatcact taatatggcc
    13501 tctgcatcat aaagctgatc tcttatgctc tgcaatgctt catcatactc tgtcacctgc
    13561 tccaccgacc gaagggctct tgtaactgca tctgcctgaa ggctgtcata ggcttcattc
    13621 agattttcag caatgcgtct tgcatgggag aaacggcggt aacgagcggt cagctcctcc
    13681 tcctcccctt cttttatatt gccctcttca atctcctgga tttcaaaacg caggaagtcc
    13741 gcttctctga gtctggcgtc atgatccatc tcaaaggagg atagccgttt ctttagaata
    13801 cagtaatttt tgtattcctc ttccatcttc ttttttgccg gttgggtttg agccttgaca
    13861 taggcatcca ggatttcaag atgttttgat ttatacagca gagactgatg ctcatgctga
    13921 ccgtgaatat ctaaaagcaa gcctgtgatc tgtctcagcc ttgctgccgt aactgtctca
    13981 tcattgatgc ggctgatact gcgtgacggg gtgattttcc ttgagatgat caccaggcca
    14041 tcctcggtag gttctgtatc cagttcccgc agcgcccgca gcctgtcttc tcccttgact
    14101 gaaaaaacga gctctatata ggcataatcg gctccgtggc gtatgctgtc tctggaagcc
    14161 tttcctccca gagccatact cacggagccg attataatgg atttaccggc gccggtctcg
    14221 ccggtaagaa tatttaggcc ctcgccaaac tccacatctg ctctttcaat aagggccaga
    14281 tttttaacgt gcagctctaa taacatatcg atcagtcctc caccatcttt ttgatctttt
    14341 ccatcacgat gatcgtgtca tccacactgc gtaccgcaca catgatggtg tcatcccctg
    14401 caatacagcc tactacctcc tgaaaatgga tggcgtccag ggcggccgcc actgccattg
    14461 ccataccggc tacggtcttg atcacaagaa tgttctgtgc catatccatg gacataaaac
    14521 catcctttaa aatacggata tatttgatcg cagaggttcc tcttggactt tcaagaacca
    14581 tataccgttg tcttccgttt tctccctgaa tctttgtaag ctttaattca cggatatctc
    14641 tggaaacagt cgcctgagtc acattaaaac cagactggtt aagcttttca gccagttcct
    14701 cctgcgtttc gatctcatat ttcccgatca gttccacaat tttactgtgg cgttctacct
    14761 tcatctatct ttcctccttg ttaagctctg caaagcgcag agcttttgtg gctgtttctc
    14821 agattccgct taaatgatcg cttaaattct gcataaacga ctgctttttc cttcggatca
    14881 tcactgtctc caaagcggaa cgctctgcga tcaggcagtc ccccgtctcc agatctgccc
    14941 aggaatctcc gtcaaaggaa actcttccgc tgcttaatac cccgatctgg atccgatcct
    15001 ctgctgcgag cacaatgctt ctcgcattta gggaatgagg acacaccgga gtcagggcca
    15061 tcatccggcc tgccggagcc atcactggcc caccggcgga caggttgtag gccgtggaac
    15121 cggtgggcgt tgccaccagg actccatctg cgctgtaatg gtttaagtat tcatcattca
    15181 ctgatacctg ataccggagt acccgcaggg gaacccggct ggtaacagcg atttcattta
    15241 gcgcaatatc ttgaaatacg ggttttccct gatgaaacac tgttccccgt atcatcattc
    15301 tccgctccag ctcaaaacgg ttctcgatca aagaagcaat ggcttcttca atctcatcct
    15361 gagagctgat ctggtttaaa aatcccaaat ggccccggtt tactcccaaa agagggatat
    15421 tcctgcctgc cagatctctg gccgcctgga tcagggtgcc gtctccgcca atggttatca
    15481 cacattctgt atcctccgga accaactcag aaggcgtatg ctttccgtat ttattatgct
    15541 gagccagggt gagaaaacgg cactcagccc ctcttttttc caggcattcc cggatatacg
    15601 ccctggtttc aggaacaaac tctttcgcca gattggcaat caaaaaaaat cgtttcatct
    15661 gccacttacc gccatttcct atgaacgatc cagagtttga tgtgcctcag ctaccactgc
    15721 cttcggatcg gcagccgctt cccggcgcac cgcatactct gcctcctgat cctctggatg
    15781 cttctgaaga tagagcaggt actcaatatt tccttccggc cccttgatcg gagaaaattc
    15841 cagattcagg gcctcaaatc cgatggaaag cgcataatcg atcaccatgt cgatcacttc
    15901 caaatgaacg gccggctctc gaacaacgcc ttttttccct actttttccc tgcctgcttc
    15961 aaactgcggt ttaatcaggc agacgatctg cccattgtcc tctaaaagag ccttcacagg
    16021 tcccaaaacc ttagtaagtg aaataaagga cacatcaata cttgcaaagc caataggatc
    16081 tgcaatatcc tccggagcca catagcggat attggttttc tccatgcaga ccacacgctc
    16141 atcattgcgc agcttccagg caagctgtcc atggcccaca tcgacagaat acaccttcac
    16201 cgctccattc tgcagcatac agtctgtaaa gccgccggtg gaagccccta catccatgca
    16261 gatctttcca tccaggcgga cgccaaaacg atccatggcc ttttccagct tcagccctcc
    16321 gcggcttaca tattttaaag tactgcctct gacctcaatg ggcgccgttt cctcaaaagt
    16381 cgttccggct ttatcctcct tctgcccatt tacatacaca ataccggaca tgatcaaggc
    16441 ctttgccttt tcccgtgact ctgccagatt acgctttaca agcagtacgt ctaatcgttc
    16501 tttcatctgt atttctcctg tctttcattc agacggtctc tgccgttctt ctccgttttc
    16561 ttccctgtgg ataccggaaa gccagccctc ttccttcagt cttcggataa tggaatcgct
    16621 gtcaattccc agtcctttcc gaagaagcga tacatttcca tgctccacat atgcatccgg
    16681 aagagcaata ttcacaacct taacctccgg ataatggctg tggatatagg cagtcactgc
    16741 aaggccatat ccgccctgaa gcacattttc ctccagggtc acaagcaggc ggtggttttt
    16801 tgccaggcgc tctgtcaatt ctttatcaaa cggttttaca aaacggccgt ttgccaggct
    16861 gcaggaatac ccctcttcct taagcttttc cctgatatgc tctgccgtgc ttaccatgct
    16921 tccaaccgcc agaagcgcaa tatcgctttc ctcacagagc agctctccct ttccgtagac
    16981 aatgggctgc ctgtattccc taagccccct gtaagcggtt cctctgggat aacggatcgc
    17041 aaggggtgca ttgtaatcca tggcaaattc catcatagcg cgcagctccc agagattttt
    17101 cggagccatt accgtcatat tggggatgga ggtcagatag gaataatcaa agattccctg
    17161 gtgggtctct ccgtcgcttc ccaccagtcc tgcccggtct acggcaaaaa ggaccggaag
    17221 attctggata catacgtcat gaagcacctg atcgtatcct ctctgcaaaa aggaggaata
    17281 gattgccacc acaggacgca ggcccgcagc cgccatgccc gcagcgcttg tcactgcatg
    17341 agcctccgca attcccacat caaaaaagcg gtccggaaac ttttttccaa aagctgcaag
    17401 tcccgtaccg tcaggcatag ccgctgtcac ggctaccagc tttggatggg tttcccccag
    17461 ctggcacagc tttctggaaa atacctctgt ataggagggt tccttctttt tcccattttc
    17521 ttttcccgtt gcaatatcaa aaggacccac accgtgaaat atggaaggat tcttctccgc
    17581 aggtttatat ccctttccct tttttgtgat cacatggatc agaactgcgt ggtctaattt
    17641 cttagcctct ttaagaacct tgcacaggac aggtatattg tggccgtcca caggacccag
    17701 gtaggtgatc cccatatttt caaaaagcat tccaggaata aacagctgct taatgctgtc
    17761 cttggtgcgc tttagcttgt cgatcaccgc atttcccacc aacggtaccc gctctaaagc
    17821 ctccacgata ttctttttta aatcattgta gccggttccg gttctgagtc cgcttaaata
    17881 cctggacata ccgcctacat tttctgatat agacatttca ttatcattta agataataat
    17941 gaaattcttt ttcatacggg cagcattatt taaagcctcg taagccatgc cgccggtgag
    18001 cgcgccgtct ccgatcacgg acaccacccg gtaatcctct cccaacagat cccggccctg
    18061 ggcaatacca aggccggcag aaatagaggt agagctgtgg cccgtgtcaa aggaatcata
    18121 ggggctttcc tttcgtttag gaaagccgct gatccctcca tactggcgca gctcgtcaaa
    18181 ctcagccatg cgtccgctta atatcttatg ggtataagac tgatggccca catcccagat
    18241 aatcttatcc tgaggcagat caaatgtttt aaacagagca attgtaagct ccaccacgcc
    18301 taaattagaa gccagatggc ctcctgtacg gctgatcttt tcgatcagaa aggtgcgtat
    18361 ctcttctgcc agaatggcca gttcacggga tgaaagctgc tttaattcct ccggcccctt
    18421 tatctgttcc aatatcatag gctcgcactt ccttcatttt tatttcatta tctctctctg
    18481 tttaccagaa ggcggatcag ctgctccaga aattcattat ttccgggaag cttatccaga
    18541 tccccaatag cggctgctga aagagcctct acctctttcc tggcctttga aagcccttct
    18601 aaagttacat aagtggtctt ctggttcttc tcgtcgcttc ctgccgcttt tccggtgaca
    18661 gaggcatctc cggtcacatc cagaatgtca tcctgaatct ggaaagcaag tcctaccttt
    18721 ccagccagat cctctacaat cttacagtcc tcatctgcgg ctcctccaag aacagctcct
    18781 atgagcatcg ctgcctgaag cagggctccc gtctttaagc ggtagataaa atccagctta
    18841 tccttgggaa cgagacctcc tgccagctct acatctaccg tctggcctcc gatcataccg
    18901 taaacgcccg tctttgctgc caggatcccc attgcctttc cggctctcaa aagcgcatct
    18961 gcatcctctg ccgcatcaaa ggccttggat gccgtctcaa aagcgtacat catcagagca
    19021 tctcccgcaa g
//
LOCUS       DBMDUH010000036        18929 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_1756_length_18929_cov_5.056458, whole genome shotgun sequence.
ACCESSION   DBMDUH010000036 DBMDUH010000000
VERSION     DBMDUH010000036.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 18929)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 18929)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18929
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1756_length_18929_cov_5.056458"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1130
                     /locus_tag="ACZPCG_04865"
     CDS             <1..1130
                     /locus_tag="ACZPCG_04865"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003523467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1284440.1"
                     /translation="FNRQESEKKTFEKENGSLVQMQVFVGKISALLNPVTYVIVNLAA
                     VAVIWVGAERTDAGIITQGQVIALVNYMSQILTELVKMANLIILISKAVACMKRVDGV
                     FELESSLRDGSDLKPAGAEKQSGPMVEFKRVSFSYGETQSEVLTDLSFCAMKGQTVGV
                     IGGTGSGKSTLVNLIPRFYDVGKGMVLVDGRDVKDYSMKELRSIIGVVPQKAVLFKGS
                     LKDNMRWGKADASDEEIWEALEIAQAKEVAEKKGQGLEFMIDQGGRNLSGGQKQRLTI
                     ARALVRRPRILILDDSASALDFATDARLRRAIREKTGDMTVFIVSQRVSAIQSADCIL
                     VLDDGHLAGMGTHKELLSSCEVYREICLSQLSNEEVERDEQ"
     gene            1120..2895
                     /locus_tag="ACZPCG_04870"
     CDS             1120..2895
                     /locus_tag="ACZPCG_04870"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1284441.1"
                     /translation="MSSKGKLDKTKSIQTLKRILLYIKDYRAAVLLSLFLAVTTVAST
                     LYIPILTGQAVDQIVGRGQVDFQQLIQILKRILIVMAVTAVSQWLMNHINNTITYRVS
                     MDIRTRAFDHLEILPLSYVDSHPAGDMISRIITDIDQFSEGLLLGFTQLFTGALTILG
                     TLLFMLSIQPFITAAVVVLTPVSFFVASFIAKKTFVMFKQQSEARGELTTLTDEMLGN
                     MKVVQAFGYEKEAEKRFQEINSRLAGCSLKATFFSSITNPATRFVNSMVYAAVGITGA
                     WGVIRGLLTIGQLTSFLSYANQYTKPFNEISGVVTELQNALASAARVFELMDEPAIPE
                     DGPEAVVLKDVDGNVALEHVSFSYQPERPLIEDLNLSVKPGQRVAIVGPTGCGKTTVI
                     NLLMRFYDVKSGSVRVEGRDIRDITRQSLRASYGMVLQETWLKAGTIRENIAYGRPEA
                     SEEEIIRAAKEAHAHSFIMRMPQGYDTVINEDGGNLSQGQKQLLCIARVMLCLPSMLI
                     LDEATSSIDTRTEIRVQKAFSRMMEGRTSFIVAHRLSTIREADVILVMRDGRIVEKGR
                     HKELLRKNGYYAEIYNSQFAPSTGE"
     gene            complement(2907..3593)
                     /locus_tag="ACZPCG_04875"
     CDS             complement(2907..3593)
                     /locus_tag="ACZPCG_04875"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HIR1284442.1"
                     /translation="MITHIFLDVDNTILDFNKAEAIALEKTLSALSVPHSPEVIRRYS
                     QLNLAQWKLLEQGKLTREQVKVRRYRLLFDELKTDASPEEAARIYEGLLGQGHYFMDG
                     AVEMLQALYGSFHLYLATNGTASVQKSRLKSAEIEPFFEQIFISEELGCNKPETSFFE
                     RCFATIPDFKKEEAVMVGDSLTSDILGGIRAGIRTIWFNKDLKESSGEIRPDHTIKAL
                     SELPDLLHSM"
     gene            3730..5100
                     /locus_tag="ACZPCG_04880"
     CDS             3730..5100
                     /locus_tag="ACZPCG_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HIR1284443.1"
                     /translation="MESDPQSASIITQLLILLVLTLVNAFFSGSEMAVVSVNKNKIHR
                     LAEQGNKNAALIERLMEDSTVFLSTIQVAITLAGFFSSASAATGIAQVLAVKMSQWNV
                     PYSQTAAGVLVTILLAYFNLVFGELVPKRIALQKAEGFSLFCVGPVYFISRIMNPFIH
                     LLTFSTSGILKLMGMHSETLETDVSEEEIKSLLETGSETGVFNEIEKEMITSIFSFDD
                     KKAREVMVPRQEMVAIDIEEPLESYLDEILQSMHSKIPVYEGDMDNIIGILSTKLLVI
                     QARERSFETLDIRSILLPPYFVPENLRTDALFEQMQARKEKMAILVDEYGGVSGMVTL
                     EDLIEEIVGDIHEKYEEEEPEIQELEPHRVYRIAGSISLFDLKEKLHLHMDSTCDTLS
                     GYLMELLGHIPEEGELPLTAQTEEAYFEIESVEDRVIDKVKLTLRDLPDAIEEEEILQ
                     KISRQM"
     gene            5208..6356
                     /locus_tag="ACZPCG_04885"
     CDS             5208..6356
                     /locus_tag="ACZPCG_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HIR1284444.1"
                     /translation="MSFLLEGILAVTWQQLVMYVVGIVLIWLAVKKEYEPSLLLPLGF
                     GAILVNLPYSGVIDQMVQGKVPAAGIIQWLFETGIEASEAMPILLFIGIGAMIDFGPL
                     LSQPVLFLFGAAAQFGIFAAILIACLLGFDLKDAASIGIIGAADGPTSILVSQVLGSN
                     YMGPIAVAAYSYMALVPIIQPFAIRLVTTHKERCIHMEYNPKSVNKTLRIAFPIAVTI
                     IVGFISPQSVALVGFLMFGNLLRECGVLNSLSQAAQNELANIITLLLGITISFSMKAE
                     QFVNPATLMIMALGLVAFVFDTIGGVLFAKLVNVFLKMAGKKPVNPMIGGCGISAFPM
                     SSRVVQRLAAEEEPGNIILMQAAGTNVSGQVASVIAGGLVISIVTQYL"
     gene            6369..6497
                     /locus_tag="ACZPCG_04890"
     CDS             6369..6497
                     /locus_tag="ACZPCG_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002573576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG-related small transporter subunit"
                     /protein_id="HIR1284445.1"
                     /translation="MNNMAIALEIMAKGMGGIFVAIFVIMLAVIVLGKITGGKKDE"
     gene            complement(6586..9504)
                     /locus_tag="ACZPCG_04895"
     CDS             complement(6586..9504)
                     /locus_tag="ACZPCG_04895"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF019735.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide lyase 8 family protein"
                     /protein_id="HIR1284446.1"
                     /translation="MYKKALIAALISCMTVSAAGCQMNSTKTDTIPDSTADINAAPTA
                     EPAEGESWAEPVVLVKGESTRLPLPANFKGEIRWTVTNGMKTVGGSQKKYPLSDGETD
                     GERTAGYASAEHLLKIEPDGTITALEKGNHLFAVAEDKNGNRRIFPMEVRIFSEDQLP
                     ETSKEDFQTLRTRYVERLTGGNIDISSPAAAAMIQATDQACDNAWNSYLYKGSGCAGV
                     PWAEDLAGGDEENYKNDAAKFRKSYQKAEAMAKAYASPSSSYYKNEALLTDIVNILDW
                     LTDNCYYPRSETDNWWNWEIGMPKNVLPAVLYIYDDIPKEQSRKFVKGVEFFMPDPFH
                     LAVLGTASTLPSGYAPAASANLMDCAYTSVGLGLVCEDSEYLALAKQAAATTLERFQK
                     PELKDGNYVYANGFYEDGSYIDHGTVPYTASYGIDFLKGSVRLSSLLADSPWALSPEN
                     AKMLESYVVKGYLPSIYQGAALDMLRGRAISRPDLTDRAAGQQIIEMIVQTIDIVSPE
                     TAAQIRGAVKTWVSQSGEEQFLAGVKDISVFSSLKEIMADDTIDTSSAEAAMHQVYPY
                     MDRTIHRTTDYLFGISMYSSRISNCEIMNDENLKGWFTGFGMTYLYNDDKQQYTDHFW
                     DTVDPLRLPGTTIVAEEVDNGEPDSSGFHQGGDFLSPENWVGGTSIGTTGVSGMKVNG
                     TAVSNGDQSNASSTVYADSFRAEKSYFMFDDEIVCLGAGIQNSGSDAPVITTVENRKL
                     NSGGSNQISSDAGEWIMPEEQIISPQWLHLKGNTETGSDIGYYFPEKQDVHIMGSTRT
                     GSYHQIKGDLKTEEETAERSYFTCWFDHGAAPENDTYSYVLLPDMTSEETKAYSQSPD
                     MEILENSEKLQAVNDHSTNTLAVNFWDEAGGQTDRISCDSQASVMVQENNGAFTIALS
                     DPTMLNTGTICLKLQELPISGKLIEADDGITIRTENGSVYLEFNVNGMCGKTMRAVIQ
                     "
     gene            complement(9846..10943)
                     /gene="ychF"
                     /locus_tag="ACZPCG_04900"
     CDS             complement(9846..10943)
                     /gene="ychF"
                     /locus_tag="ACZPCG_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HIR1284447.1"
                     /translation="MKLGIVGLPNVGKSTLFNSLTKAGAESANYPFCTIDPNVGVVPV
                     PDERLDKLTAMYNSEKTTHAVIEFVDIAGLVKGASKGEGLGNQFLSNIREVDAIVHVV
                     RCFDDPNVIHVDGSVDPVRDIETINLELIFSDIEILERRIAKQSKGAFNNKELAKEVA
                     LLKAIKAHLEDGKLARSFEPEDEDDRAFINTLDLLTWKPVIFAANVSEDDLADDGASN
                     RYVSEVRQFAAENDCEVFVICAEIEQEIAELEEDEKKMFLDDLGLTESGLEKLIAASY
                     RILGLMSYLTAGPQESRAWTIKVGTKAPQAAGKIHSDFERGFIRAEVVNYDDLMACGS
                     MNAAKEKGLVRSEGKEYVMKDGDVVLFRFNV"
     gene            11343..12854
                     /locus_tag="ACZPCG_04905"
     CDS             11343..12854
                     /locus_tag="ACZPCG_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HIR1284448.1"
                     /translation="MYIAIDLKSFYASVECVERGLDPLKTNLVVADISRTEKTICLAV
                     SPSLKAHGISGRARLFEVVQRTKEVNALRRLNAPGRKLTGESWKDDELKADPSLALSY
                     IIAPPRMAEYMKWSARIYNIYLKYIAPEDIHVYSIDEVFMDVTQYLEIYGMTPAQLAG
                     KIIRDVLAQTGITAAGGIAPNLYLCKAALDIVAKHVSPDEDGVRIAQLNEMSYRRLLW
                     DHRPLTDFWRVGKGYARKLEENGIYTMGDVARCSLGKQGEAHSEELLYRLFGINAELL
                     IDHAWGWEPCTIQDIKSYKPQTNSLCSGQVLRGPSSWDQARLVVKEMADQLALDLVEK
                     GLVTDQIVLTVGYDRENLTDPLRRGAYGGEITSDRYGRPVPKHAHGTRNLDQPTSSAR
                     KLREAAAALFDRIADPGLLVRRLYVTAGRAVPEENIKKEEVYEQLDLFTDYMALEQQR
                     KKENEALEREKKMQQAMVDIRKKFGKNAILKGMNLEEGATARERNRQIGGHKA"
     gene            12851..13375
                     /locus_tag="ACZPCG_04910"
     CDS             12851..13375
                     /locus_tag="ACZPCG_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YolD-like family protein"
                     /protein_id="HIR1284449.1"
                     /translation="MIGKNNRLYTGPDAEWERRVRESMEAGKEWAFKVPSHAYDDIIH
                     LPHHVSSVHPPMSLHDRAAQFSPFAALSGFEGEIRETERLTEGKIELDEEEAGRLDER
                     LRQIRQHLSVCPRVSLTWFLPDERKNGGSYERAAGRVKKLDPVQGLLVFTDGRKIPLE
                     QIVWIEEETGISDF"
     gene            complement(13372..14253)
                     /gene="sdaAA"
                     /locus_tag="ACZPCG_04915"
     CDS             complement(13372..14253)
                     /gene="sdaAA"
                     /locus_tag="ACZPCG_04915"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015527733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HIR1284450.1"
                     /translation="MNTDFKNGKELLEFCEKEKISISRAMLLRESDQTEKTESSIYTR
                     METAWNIMKEAALSPISSPGKSIGGLIGGEAKQLNQLFKAEKNICGTAVSRGITYAMA
                     VLEVNASMGLIVAAPTAGSSGIVPGVVLSLKEEHDFSDSQAVDALFNAGAIGYLAMRN
                     ATVAGAVGGCQAEVGVASAMAASAAVELMGGSPRQCLYAASTVLMNMLGLVCDPVGGL
                     VEYPCQMRNASGVANAMIAAELALAGIPQLIPFDEMLEAMYTVGKKLPGELRETAMGG
                     CAVTPTGCQYGCNGHCS"
     gene            complement(14299..14967)
                     /gene="sdaAB"
                     /locus_tag="ACZPCG_04920"
     CDS             complement(14299..14967)
                     /gene="sdaAB"
                     /locus_tag="ACZPCG_04920"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HIR1284451.1"
                     /translation="MAFISVFDVIGPNMIGPSSSHTAGADSLAFLAQKMINGPLKKVD
                     FTLYGSFAKTYRGHGTDRALLGGIMGFTTDDKRIPDSFSIAEEKGLEYHFHINETEEV
                     DHPNTVDITMVNEDGQTLTVRGESLGGGKIRISRINQVDVDFTGEYSTAIVIHQDTPG
                     VVAHITRCLAEENINIAFMKLFREEKGQTAYSIVESDDELPDSVSEKICQNPSVRNVM
                     LVHL"
     gene            15136..16017
                     /locus_tag="ACZPCG_04925"
     CDS             15136..16017
                     /locus_tag="ACZPCG_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1284452.1"
                     /translation="MEIRQIQSFVTITQTGSFSGAAQLLGYSQSAITVQIRLLEEELG
                     VRLFDRMGKKIGLTSQGKRFLLCADEILKQVQMAGELKNTGEELKDPLHIGTLTSICF
                     SKFPPILQYFREHHPRVPIRITTASPGELIDMMEHNQLDLVYLLDRSRYSDRWEKVME
                     VPEPVMFVASPRFHLAGQKEIYLEQLLKEPFLLTERNENYRRELDQFLESRGVTLTPF
                     LEISSTEFIIRMVCENRGITYLPYFAVEKNIEEKNLMVLNVPDFKLQMYRQIFYYKNK
                     WKTPEMEEFIRLARELR"
     gene            16310..16996
                     /locus_tag="ACZPCG_04930"
     CDS             16310..16996
                     /locus_tag="ACZPCG_04930"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HIR1284453.1"
                     /translation="MDQLVESLMEELNCNTVFTIPIFGGIQVFESVVVTWIIMAVLMG
                     ASLILTRHLKVRNISTRQAALESGFSFVYSFFSDLLGEEGKEYIPYLITVAVYIAIAN
                     MIGLLGFKSPTKDLNVTASLAVMSIILVEAAGIRSKGVKGWIRSFAEPIPIVAPINVL
                     ELFIRPLSLCMRLFGNVLGAIVVMALIKYLVPLVLPLPFSGYFDIFDGVIQAYVFVFL
                     TSLYIKEAIE"
     gene            17026..17250
                     /gene="atpE"
                     /locus_tag="ACZPCG_04935"
     CDS             17026..17250
                     /gene="atpE"
                     /locus_tag="ACZPCG_04935"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HIR1284454.1"
                     /translation="MSGIIAIGAAVAVITGIGAGIGIGMATSKAVDAIARQPEADGKI
                     TKALLLGCALAEATAIYGFVIGLLIILFLK"
     gene            17276..17767
                     /gene="atpF"
                     /locus_tag="ACZPCG_04940"
     CDS             17276..17767
                     /gene="atpF"
                     /locus_tag="ACZPCG_04940"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006778930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HIR1284455.1"
                     /translation="MLRISVWNIAFTIINILVLFFFMRHFLMKPVMEILEERKKLVEK
                     DLDEADRSKIQAAELKERYEASLAAAEKEADCIVAKARQQAQSEYERMMEQADQDARK
                     KLQASEKTMELEREKVLNELRTSVTGLAMTAAARLLTEKNSPEWNKRQYDVFLAGEGE
                     KND"
     gene            17760..18266
                     /locus_tag="ACZPCG_04945"
     CDS             17760..18266
                     /locus_tag="ACZPCG_04945"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HIR1284456.1"
                     /translation="MTEQTRMYGRVLYELHLPEKMVCEMVKVIRENPLLVQILSDPVL
                     PSFKKDRIIEKVWRASEFTEIVPSFLKKVCAAGCIGSMDSILSVWEQCLRDEKGIIQA
                     ELFYVTPPDEEELQKIRRFLCGKYGKKEVQFILASAPELIGGFLLRTENVEYDYSLKS
                     RLEELFGI"
     gene            18286..>18929
                     /locus_tag="ACZPCG_04950"
     CDS             18286..>18929
                     /locus_tag="ACZPCG_04950"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585226.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HIR1284457.1"
                     /translation="MSTISSQEVISIVKGEIEDYDAHAGRIRETGSVIWVGDGIAIVY
                     GIDHAMYGEIVIFENGVRGMVQDIRKNEIGCILFGKDTGIMEGTKVTRTRKRAGVPVG
                     SGFLGRVVNALGDTIDGLGPVKEEDYYPVEREAPGIVERKSVSVPMETGILAIDSMFP
                     IGRGQRELIIGDRQTGKTSIALDTIINQKGKDVICIYVAIGQKASTVAKLVNTL"
BASE COUNT         4964 a         3970 c         4890 g         5105 t
ORIGIN      
        1 cctttaaccg gcaggaaagt gaaaagaaaa cgtttgagaa ggaaaatggc agcctggtgc
       61 agatgcaggt atttgtggga aaaatttccg ctcttttaaa tcccgttacc tatgtgatcg
      121 tcaatctggc tgctgtggct gtgatctggg tgggggcaga acgcaccgat gcaggcatca
      181 ttactcaggg acaggtcatt gcgcttgtaa actatatgtc tcagatcctc acagaactgg
      241 ttaaaatggc caatcttatt atcctgatct caaaggcagt ggcctgcatg aagcgggtgg
      301 acggagtatt tgagctggaa agcagcctaa gggacggctc cgatttgaag cctgccgggg
      361 cagaaaaaca aagcggtcca atggtggaat ttaagcgcgt aagcttctcc tatggggaga
      421 ctcagtcgga agtccttaca gatctttcat tctgcgccat gaaggggcag acggtgggag
      481 tcataggagg caccggttcc ggcaaatcta cgcttgtaaa tctgatcccc cggttttatg
      541 atgtgggaaa gggaatggtt cttgtagacg gccgtgatgt gaaggattat tctatgaaag
      601 agctccgttc catcatcgga gtggtgcctc agaaggcggt gctttttaag gggagtctta
      661 aagacaatat gcgctgggga aaggcggatg cctcggatga ggaaatctgg gaggcattag
      721 aaattgccca ggcaaaggaa gtggcagaga aaaagggaca gggactggag tttatgatcg
      781 atcagggagg ccgaaatctg tccggaggcc agaagcagcg tctgaccatt gccagggctc
      841 tggtgcgcag gccgcggatc ctgatcctgg atgacagcgc atcagccctt gattttgcca
      901 cagatgccag gctgagaaga gcgatccggg aaaaaacggg ggatatgacg gtatttatcg
      961 tatcccagag ggtatcggct atccaaagtg cagactgtat tctggtactg gatgacgggc
     1021 atctggccgg tatgggaacc cataaagagc ttctttcttc ctgtgaggtt tatcgggaaa
     1081 tctgtctgtc gcagctttca aacgaggagg tggaacggga tgagcagtaa gggaaaatta
     1141 gataaaacaa aaagcataca gaccctaaaa aggatcctgc tctatataaa agattaccgt
     1201 gcggcagtgc ttttgtccct ttttctggca gtgacaacgg tggcttcaac tctctatatc
     1261 ccgatcctta cgggacaggc tgtggatcag atcgtgggaa gaggacaggt ggactttcag
     1321 cagctgatcc agatactgaa acggattttg atcgttatgg cagtgacagc agtaagccag
     1381 tggctgatga accacatcaa caataccatt acctaccgtg tttccatgga tatacgtacc
     1441 cgggcattcg atcatctgga gattcttcct ttaagttacg ttgattctca tccggcggga
     1501 gatatgatca gccgtattat tacagatatt gaccagtttt cagaaggcct gctgttaggc
     1561 tttacccagt tatttaccgg tgctttaacg attttgggaa ctcttttatt tatgctgtcg
     1621 atccagccat ttattactgc tgcggtggtg gtgttgacgc cagtatcctt ttttgtagcc
     1681 agttttattg caaaaaagac ttttgtgatg tttaagcagc agtctgaggc cagaggtgaa
     1741 ctcacaacac tcacagatga gatgctggga aatatgaagg tcgttcaggc ctttggatat
     1801 gagaaggagg ctgaaaaacg ttttcaggag attaacagcc ggttggccgg atgcagcctt
     1861 aaggccacct tcttttcttc cattaccaat ccggccaccc gttttgttaa cagcatggtc
     1921 tatgcggcgg ttgggattac cggagcctgg ggagtgatcc gtgggctttt aaccatcggg
     1981 cagctgacca gctttttaag ctatgccaat cagtatacaa agccatttaa tgaaatttcc
     2041 ggagtagtga cagagctgca gaatgcgttg gcctctgctg ccagagtttt tgaacttatg
     2101 gatgaaccag ccatcccgga ggatggaccg gaggctgttg ttttaaagga tgtggatgga
     2161 aatgtagctc tggagcatgt gagcttttcc tatcagccgg agcgtccttt gatcgaggat
     2221 ctgaacctga gcgtcaaacc ggggcagaga gtggcgatcg ttgggccgac gggctgcgga
     2281 aagactaccg tgatcaatct tcttatgcgt ttttatgacg taaaatcagg aagcgtacgg
     2341 gtagaaggga gagatatccg ggatataacg agacagagtt taagagccag ctatggcatg
     2401 gttcttcagg aaacctggct gaaggccgga accatccggg aaaatatcgc ttacggaagg
     2461 ccggaagctt cagaggaaga gatcatccgt gcagcaaaag aggcacacgc ccacagcttt
     2521 atcatgcgta tgcctcaggg atatgatacg gtcataaatg aagatggagg gaatctgtct
     2581 cagggacaga aacagctttt atgcattgcc agagtgatgc tctgcctacc gtctatgctg
     2641 atcctggatg aggccacctc atccattgat acccgcacag aaatccgggt acagaaggca
     2701 ttttcacgga tgatggaggg aagaaccagt tttattgtgg cccaccgtct ttctaccatc
     2761 cgggaagcgg atgtgatcct ggtcatgcgt gatggccgta ttgtggaaaa gggccgtcac
     2821 aaagagctgc tgcgtaaaaa cggctattat gcagaaatct ataatagcca gtttgcccct
     2881 tccacggggg aatgaccgtt tactctttac atggagtgaa gaaggtccgg tagctcgcta
     2941 agagctttga tcgtatgatc ggggcggatt tctccggaag attcttttaa gtccttatta
     3001 aaccatatgg tgcggatccc tgcacggatt ccgcctaaga tatcagaggt taggctgtca
     3061 ccgaccatga cagcctcctc ttttttaaaa tcagggattg ttgcaaagca gcgttcaaaa
     3121 aatgaggttt ccggcttgtt gcagcccagt tcctcggaga tgaagatctg ttcaaaaaac
     3181 ggttcgatct ctgcactttt gagtctgctt ttctgcacag atgctgtgcc gtttgttgcc
     3241 agatagagat gaaaggagcc ataaagcgcc tgaagcattt ccacagcccc gtccataaag
     3301 taatggccct gtcctaaaag gccttcataa atacgggcgg cttcctcagg ggacgcatcc
     3361 gttttaagct cgtcaaagag aagccggtag cggcgcacct ttacctgttc ccttgtaagc
     3421 tttccctgct ccaaaagctt ccactgggcc agattgagct ggctgtagcg gcggatcacc
     3481 tctggagaat gtgggacaga cagggcggaa agggtctttt ccaatgctat agcctcggct
     3541 ttattaaaat ccagaatggt attatctaca tcaagaaaaa tatgagtgat catttttacc
     3601 tccaagtatg tatcattgat gttactttaa cacagtttta acgcttcgta aataaaactt
     3661 ttagctgaca aattcctcct ttttcagtat aatgtacctt ataccatcaa aaaggaggaa
     3721 ttttttatta tggaatccga tccgcaatcg gccagtatca tcacacagct tctgatcctt
     3781 ctggtgctga ctcttgtcaa tgcattcttt tcaggctctg agatggctgt ggtctctgtc
     3841 aacaaaaaca aaatccaccg tctggcggag cagggcaata aaaacgctgc tctgatcgaa
     3901 cgtctgatgg aggactctac ggtctttctt tctacgatcc aggtcgcgat cactctggca
     3961 ggcttctttt ccagtgcgtc tgcggctacc ggtattgctc aggtactggc agtaaaaatg
     4021 agccagtgga atgtgcccta cagccagacg gcagcaggag tgcttgttac cattcttttg
     4081 gcctatttta atctggtttt tggtgagctt gtgccaaaga ggattgctct tcagaaggca
     4141 gagggcttta gcctgttctg cgtaggcccg gtatatttca tttccaggat catgaatccc
     4201 tttatccatc tgctcacctt ttccaccagc ggtattttaa agcttatggg gatgcacagt
     4261 gaaacactgg agacagacgt atcagaggaa gagatcaaat cccttctgga aacaggaagt
     4321 gagacagggg tatttaatga aattgaaaag gaaatgatca catccatttt ttcttttgat
     4381 gacaagaagg ccagagaggt catggtgccc agacaggaaa tggtggcgat tgatattgaa
     4441 gaaccgttgg aatcctatct ggatgaaatt ttacagtcta tgcattccaa gattccggtt
     4501 tatgagggag atatggataa tatcatcggc atcctgtcca ccaagctttt agtgattcag
     4561 gcgagagaac gctcctttga aacactggat atccgttcaa tcttgctgcc gccgtatttt
     4621 gtgccggaaa atctccgcac agacgccctg tttgaacaga tgcaggccag aaaggaaaag
     4681 atggctattc tggtagatga gtatggaggg gtttccggta tggtgaccct ggaagatctg
     4741 atcgaggaga ttgtgggaga tatccatgaa aaatatgagg aggaagagcc ggaaatacag
     4801 gagttagagc ctcaccgggt ttatcggata gcaggcagta tttctctttt tgacctgaag
     4861 gaaaaactcc atcttcatat ggacagcacc tgcgatacgc tttccggcta tctgatggag
     4921 ctgttaggcc atattcctga agagggagag cttccgctga ccgctcagac ggaggaggcg
     4981 tattttgaga tcgaatctgt ggaggacaga gtgatcgata aggtaaagct tactttaaga
     5041 gatctgccgg atgcgattga agaggaagaa atattgcaga aaattagcag acaaatgtaa
     5101 aataatcagt tataataaga gcgatgtgcg aaacggcaca aaaataccgc agatgtgtat
     5161 ggagaaaaaa gagggttttc tatacacgcg caaaaggagg agaaacgatg agttttcttt
     5221 tggagggaat cctggcagtc acctggcagc agctggtcat gtatgtggtg ggtattgtgc
     5281 tcatatggct ggcggtcaaa aaggaatatg agccttcgct gctgctgccg ttgggatttg
     5341 gtgcaatttt ggtgaatctt ccctattccg gtgtgatcga ccagatggtt cagggaaagg
     5401 ttccggcagc gggtatcatc cagtggcttt ttgagaccgg aattgaagca tcggaggcta
     5461 tgccgatcct gctgtttatc ggtatcggag ctatgattga ttttgggccc ctgctcagtc
     5521 agcctgtgct gtttctgttc ggtgcggccg cacagtttgg tatctttgca gcaatcctga
     5581 tcgcctgtct gctgggcttt gatcttaaag acgctgcatc catcggtatc atcggagctg
     5641 cggacggccc tacctcgatc cttgtatctc aggtgcttgg ttctaactat atgggaccga
     5701 ttgcagtggc agcctattcc tatatggctc tggttcctat tatccagcct tttgcgatca
     5761 ggctcgtaac tactcacaaa gaacgctgta tccatatgga gtacaatccc aagagtgtca
     5821 ataaaacact gcggatcgca tttccgattg cagttacgat tattgtaggc tttatttctc
     5881 ctcagtcggt cgctttggtc ggctttctga tgttcggcaa tctgctgcgt gaatgtggcg
     5941 tgcttaacag cttaagccag gcagcgcaga atgagctggc aaatatcatt acccttcttc
     6001 tgggaattac gatttccttc agcatgaagg cagagcagtt tgtaaatcct gcaacactga
     6061 tgatcatggc cttaggcctg gttgcttttg tgtttgatac cataggcgga gtgctttttg
     6121 caaagctggt taatgtattc cttaaaatgg caggcaaaaa gcctgtaaac ccaatgatcg
     6181 gcggctgcgg catttccgcg ttccccatgt cttcccgtgt cgttcaaaga ctggcggcag
     6241 aagaggagcc gggaaatatc attctcatgc aggcagccgg aaccaatgta tccggacagg
     6301 tggcctctgt gattgcaggc ggcctggtga tcagtattgt gacacagtat ctgtaaggag
     6361 gaaggtttat gaataacatg gcaattgccc tggaaatcat ggcaaaggga atgggtggaa
     6421 tttttgttgc tatttttgtg atcatgctgg cagtgatcgt tttgggaaag attactggtg
     6481 gcaagaagga cgaatagaaa ctgcttttag aaaaattagt gtaaaaaggc gccgctgtag
     6541 agaatgcggc gcctttttta gtttttagag cagattgccg tacgtttact ggatcactgc
     6601 ccgcatggtt tttccgcaca ttccgtttac attaaattcc agataaacag agccgttttc
     6661 agtacggatc gtgataccgt catctgcttc gatcagtttg ccggatatgg gaagctcctg
     6721 aagcttcagg cagatcgttc ctgtattgag catggtgggg tcggacaggg cgatggtaaa
     6781 ggctccgttg ttttcctgaa ccatcacaga tgcctggctg tcgcagctga tccggtctgt
     6841 ctgtccgcct gcttcatccc agaagtttac agccagagtg ttggtggaat gatcgtttac
     6901 ggcctgcagc ttctcagagt tttcaagtat ttccatatca ggagactggc tgtaggcttt
     6961 cgtttcttcg ctggtcatat ccggaaggag aacatagctg taggtatcat tttccggtgc
     7021 agcgccgtgg tcaaaccagc atgtgaaata ggagcgctca gcagtttcct cctctgtctt
     7081 tagatcgcct ttgatctggt gatagcttcc ggttctggta ctgcccatga tgtgaacatc
     7141 ctgtttttca gggaagtagt agccaatatc agagccggtt tcggtatttc cttttaaatg
     7201 aagccactgg ggagaaatga tctgctcctc aggcatgatc cattctcctg catcggagct
     7261 tatctgattg gaaccgccgc tgtttaattt ccggttttcc acagtggtga ttaccggagc
     7321 gtctgaaccg ctgttttgga ttcccgcacc cagacagaca atctcatcat caaacatgaa
     7381 gtaggacttt tctgcacgga agctgtctgc atagacagta gaagaggcat tgctttgatc
     7441 cccattggat actgcagtgc cgtttacctt cattccgctg actccggtcg ttccgatcga
     7501 ggtgccgccc acccagtttt caggagagag aaaatcgccg ccctgatgga atccgctgga
     7561 atcaggctcg ccgttgtcga cttcttctgc cacgatggtt gttcccggca gtctcagggg
     7621 atccacggta tcccagaagt gatccgtata ctgctgctta tcatcattat agagataggt
     7681 cattccgaac ccggtgaacc agcctttcag gttctcgtcg ttcatgatct cgcagttgct
     7741 gatgcggctg gaatacatac tgatgccgaa cagataatca gtggtccggt gtatggtgcg
     7801 gtccatgtaa ggatatacct ggtgcatcgc tgcttcggct gaggaggtat cgatggtatc
     7861 gtctgccatg atttctttca ggcttgaaaa tacggagata tcctttactc cggccaggaa
     7921 ctgctcttca ccggactggc ttacccatgt ttttacagcg ccgcggatct gtgcggcagt
     7981 ttccggagaa acgatatcaa tggtttggac gatcatttca atgatctgct gtccggcagc
     8041 ccggtcagtc agatcgggtc tggaaatggc tctgccacgg agcatatcca gcgcagcgcc
     8101 ctgatagata gaaggaagat agcccttgac tacgtaggat tccagcattt tggcgttttc
     8161 cggagaaaga gcccaggggc tgtctgccag cagagagctt aagcgcacgg agcctttcag
     8221 aaaatcaatg ccgtaggaag ccgtgtaggg tacggtaccg tggtcaatgt aggagccgtc
     8281 ttcataaaat ccatttgcat agacatagtt tccgtctttc agttccggtt tctgaaagcg
     8341 ttccagggta gtggcagctg cctgctttgc aagagccaga tattcgctgt cttcacacac
     8401 caggccaagg ccgacggatg tgtaggcgca atccatcagg tttgcgctgg cggcaggcgc
     8461 atagccggaa ggaagggtac tggcagtacc taaaacagcc agatgaaatg gatcgggcat
     8521 gaagaattcc acgcctttta caaatttgcg gctttgctct ttgggaatat catcataaat
     8581 atagaggaca gccggaagga cgttctttgg cattccgatt tcccagttcc accagttgtc
     8641 ggtttcggag cggggatagt agcagttatc ggtcagccag tccaaaatat tgacaatatc
     8701 cgttaagagg gcttcatttt tatagtagga actggaaggg gatgcgtagg ctttggccat
     8761 agcctctgcc ttctgataac ttttgcggaa ttttgcggcg tcatttttgt agttttcttc
     8821 atcgcctcct gcaagatcct ccgcccaggg aacgcctgcg cagccgctgc ctttataaag
     8881 gtagctgttc catgcattgt cacaggcctg gtctgtggcc tggatcatag cagcggcagc
     8941 gggagaggat atatctatat ttcctccggt cagacgctct acatagcggg tacggagtgt
     9001 ctgaaaatcc tctttggatg tttcgggaag ctgatcctct gaaaaaatcc gaacttccat
     9061 aggaaagatc cggcgattgc cgtttttgtc ctccgctact gcaaacagat gattgccttt
     9121 ttcaagagca gtaatggttc cgtctggttc gatttttaaa agatgttctg cagaggcata
     9181 accggctgtg cgttccccgt ccgtttctcc gtcgcttagc ggatactttt tctggctgcc
     9241 tcctacggtt ttcatgccgt ttgtgacagt ccagcggatt tcgcccttaa aattggccgg
     9301 gagcggcagc ctggtggatt ctccttttac aagaaccacg ggctctgccc aggattctcc
     9361 ttctgcgggt tctgcggtcg gagctgcgtt tatgtccgcg gtagaatccg gaatggtgtc
     9421 tgtttttgtg ctgttcatct ggcagccggc tgcgctgact gtcatgcagg aaatcagggc
     9481 agcgatcagt gcttttttgt acattgcatt tcctcctttt ttatgaacgg tattcatgaa
     9541 cgtttgatgc gagcagaaca aggtgttcgc ggtattgtga gtttacttca gtttaccaat
     9601 atatttacca aagtttacaa gacatatata ccattatttt accaataaat caagacaaac
     9661 tgaaaaaagt ttacaaaaaa atgggaaaac agacgaaata atgattgatc tgagcaaaaa
     9721 agaagcttat agctgtttcg gtggggatag aaagaaaaaa accgcctgct accagatggt
     9781 atatcaggcg gtaatgaagg agaatcataa aacaagatcg atctccctct ggcaaaatga
     9841 ttaaattata cattaaaacg gaacagaacc acatctccgt ccttcatgac gtattccttg
     9901 ccctcagaac gcacaagtcc cttttctttt gcggcattca tgcttccgca ggccataaga
     9961 tcgtcatagt ttacaacctc tgcccggata aagccgcgtt caaaatcgct gtggattttt
    10021 ccggcagcct gaggtgcctt ggtgcctact ttaatggtcc atgctctgga ctcctgagga
    10081 cctgcagtca gatagctcat aagtcccaga atacggtagc tggcagcgat gagcttctca
    10141 agtccggact ctgttaagcc cagatcatca aggaacattt tcttttcatc ttcctcaagc
    10201 tctgcaattt cctgctcgat ttctgcacag atcacaaata cctcacagtc attttctgct
    10261 gcaaactggc gtacctcaga tacataccgg ttggaagcgc cgtcatctgc cagatcatcc
    10321 tcggaaacat tggcagcaaa gataacaggc ttccaggtca gcaaatccag cgtgttgata
    10381 aatgcgcggt catcctcgtc ttcgggttca aagctgcggg ccagctttcc atcctccaga
    10441 tgtgctttga tcgcctttaa aagagcgacc tcctttgcca gctccttatt gttaaatgcg
    10501 cctttggact gctttgcgat ccggcgctcc aggatttcga tatcagagaa gatcagttcc
    10561 agattgatgg tttcaatgtc gcggaccgga tctacgcttc cgtctacatg aatcacatta
    10621 gggtcatcaa agcagcgcac cacatggacg atcgcgtcta cctcccggat attggaaaga
    10681 aactgatttc ccagaccttc acctttggaa gctcccttta ccagacctgc aatatctaca
    10741 aattcaatca ctgcatgggt ggttttttcg gagttataca tggcggtcag cttgtccagc
    10801 ctctcatcgg gaaccggtac aacgcctaca ttgggatcga tggtacagaa gggataattg
    10861 gcagactcgg cgcctgcttt tgtgagagaa ttaaaaagtg tactttttcc tacattcgga
    10921 agtccgacga tacctaattt catttttatc tttacctgtt cagaaaccct tgatttatgc
    10981 gggtttctgc ctttctctat atttctttta ttgtatgtta aaaaagtctg atgcagatat
    11041 aaagtatatc acagggattc cgttttttca atcactgaag tggaaatacg gggtgtttcg
    11101 cagcagttca gccctgcaaa aaagatgttt ctttttggtt tatttctggt aaactatgtt
    11161 tataggcaca cgggctgtat tttagccggt gtaaagcccg tggatccggt tgttaactgc
    11221 atgatcctgc catatgatgt acacgattta aggatacgtt ttgcagcttt gttgtttatt
    11281 atgttcgttg acagcccata aggaaaggag gttttccatt gtcccgtcca cagcaagacc
    11341 gcatgtatat agccattgat ttaaaatcct tttacgcctc cgtagagtgt gtggagcggg
    11401 ggctggatcc tttaaagacc aatctggtag ttgcggatat aagccgtacg gaaaaaacca
    11461 tctgtctggc agtgtccccc tcccttaagg cccatggcat ttccggccgc gcaaggcttt
    11521 ttgaggtggt tcagaggaca aaggaggtaa atgcccttcg cagattaaac gcaccgggaa
    11581 ggaagcttac aggggagtcc tggaaggatg atgaattaaa ggcagacccg tctctggctc
    11641 tttcttacat cattgctccg cccagaatgg cggaatatat gaaatggagc gccaggattt
    11701 acaatattta tctgaaatac attgcgccgg aggatattca tgtctattcc attgacgagg
    11761 tgtttatgga tgtaacccag tatctggaaa tttatggtat gactcccgca cagctggctg
    11821 gaaaaataat cagggatgtg ctggcacaga ccgggataac agcagcagga gggattgcac
    11881 cgaatcttta cctgtgtaaa gctgccttgg acattgttgc aaagcatgtg tccccggatg
    11941 aggatggggt gcggatcgcc cagctaaatg agatgtctta ccgcaggctc ttatgggatc
    12001 accggccttt gacggatttt tggagagttg gaaagggata cgcccgaaaa ctggaagaaa
    12061 atggaatcta taccatggga gatgtggcaa gatgttccct gggaaagcag ggagaggccc
    12121 acagcgagga actgttgtac cggctgtttg ggatcaatgc agagcttttg atcgatcacg
    12181 cctggggatg ggaaccctgc acgattcagg atatcaagtc ctataagcct cagacgaaca
    12241 gcctttgttc cggtcaggtg ctgcgcggtc cgtcctcctg ggatcaggcc agactggtgg
    12301 tgaaggaaat ggctgatcag cttgcgttag atttggtaga aaaagggctg gttacggatc
    12361 agatcgtgct tactgtgggc tatgacaggg aaaatctgac cgatccgttg cgcagaggcg
    12421 cctatggggg agagatcacc tccgaccggt atggccgtcc tgttccaaag catgcacacg
    12481 gtacccggaa tctggatcag cccacctcct cagccagaaa gctcagggaa gcggcggcag
    12541 ctctttttga ccggatcgcg gatccggggc ttctggttcg caggctttat gtgacagccg
    12601 gacgggccgt gccggaggaa aatataaaaa aagaagaggt ttatgaacag ctggatctgt
    12661 ttaccgatta tatggcgtta gagcagcaga gaaagaagga aaatgaggcg ctggaacggg
    12721 aaaagaaaat gcagcaggct atggtggata ttcggaaaaa gtttgggaaa aacgctattt
    12781 taaagggaat gaatctggag gaaggcgcta cggccagaga acgaaaccgt cagatcggag
    12841 gtcataaagc atgatcggga aaaataacag gctttataca ggcccggatg cagaatggga
    12901 gcgaagggta agagagagca tggaggcagg aaaagaatgg gcttttaaag ttccgtccca
    12961 tgcgtatgat gatatcattc atctgcctca ccatgtatcc tctgtccatc ctcccatgtc
    13021 tcttcacgac cgggcggctc agttttcgcc ctttgctgcc ctgagtggct ttgaagggga
    13081 aataagggaa acagagaggc ttacagaggg gaaaatagaa ctggatgagg aagaagcggg
    13141 ccggctggac gaaaggctca gacagatccg gcagcatctt tccgtctgcc cccgtgtttc
    13201 tcttacctgg tttttgccgg atgagagaaa gaacggaggc tcctatgaaa gggctgccgg
    13261 aagagtaaaa aagctggatc cggtccaggg gcttcttgtc ttcacggacg gaaggaagat
    13321 cccgctggag caaattgtct ggattgaaga agagacgggg atatctgatt tttaagaaca
    13381 gtgtccgttg cagccgtact gacatcccgt aggcgttacg gcacagccgc ccatggcagt
    13441 ttcccgaagt tcgccgggaa gttttttgcc tacggtatac atagcttcca gcatttcatc
    13501 aaagggaatc agctggggga ttccagccaa agccagttct gcagcgatca tggcatttgc
    13561 tacaccggaa gcatttctca tctggcaggg atattcaacc agaccgccta ccggatcgca
    13621 gaccagtccc agcatattca taagaactgt gcttgcagca tagagacact ggcgaggtga
    13681 gcctcccata agttctacgg cggcagaagc agccatggca gaagcaacgc ctacttcggc
    13741 ctgacagccg cctactgcgc ctgctacagt ggcattgcgc atggccagat atccgattgc
    13801 tccggcatta aacaaagcat caacagcctg actgtcagaa aaatcatgtt cctcttttaa
    13861 agaaagaact actccgggga caatacctga ggagcctgca gtgggggcag ctacgatcag
    13921 acccatggag gcattgacct ccagtacagc catggcatag gtaattcccc ggctgactgc
    13981 tgtaccgcag atgttttttt cagctttaaa aagctgattc agctgttttg cttctcctcc
    14041 gatgaggccg ccgatggatt ttccggggga ggaaataggg gagagggctg cctctttcat
    14101 aatattccat gcagtttcca ttcgtgtgta tatggaagat tctgtttttt ctgtctggtc
    14161 actttcgcgt aaaagcatag ctctggaaat gcttattttt tctttttcac aaaattccaa
    14221 caattctttt ccgttcttaa aatcagtatt catgatctgg attctccttt tgtatatgaa
    14281 acgtattcgg aaaatgagtt acagatgaac cagcattaca tttcgcacag aaggattctg
    14341 gcagattttt tcgctgacag aatccggaag ctcatcatca gattctacga tagagtaagc
    14401 agtctgtcct ttttcttcac ggaagagttt cataaaggca atattgatgt tttcttctgc
    14461 cagacagcgt gtaatgtggg ccacaacacc gggagtatcc tgatggatta caatagcagt
    14521 gctgtattca ccggtaaagt ctacgtctac ctgattgata cggctgatgc ggatttttcc
    14581 gccgcctaaa gattctcccc gcactgtaag tgtctgaccg tcttcattaa ccatggtaat
    14641 atctacagta ttgggatgat ctacctcttc tgtctcgttg atatgaaaat ggtattcaag
    14701 tcctttttct tcagcaatgg agaaagagtc agggatacgt ttgtcatcag tcgtaaatcc
    14761 cataataccg cccagaagag cccggtcagt tccgtgacca cggtaggttt tggcaaaaga
    14821 gccgtagagg gtaaaatcca ctttttttaa aggtccgttt atcattttct gagccaaaaa
    14881 ggccagggaa tcagcacctg cggtatggga gctggaaggc ccgatcatgt tggggcctat
    14941 tacatcaaaa acactgataa atgccataat gcagttcctt tctgttttct ggtattgcca
    15001 tagatctgga gtatagtttt atccttgacg ttactatata taaagtatat catgtagaat
    15061 aggaagaatc aaattcatgg attatataga ttgattgaaa aaaattcata acaggattga
    15121 aaaggagaga atgaggtgga gatacgtcag atacagtctt ttgtaaccat tactcagaca
    15181 ggaagctttt ccggagcagc ccagttactg ggatattccc agtcagcaat tacggttcag
    15241 attcgtcttc tagaggaaga actgggagtc aggctgtttg accgtatggg taaaaagata
    15301 ggtctaactt cccagggaaa gcgtttttta ctttgcgcag atgagatact aaagcaggta
    15361 caaatggcag gagagttgaa aaatacaggg gaagagctta aggatcctct tcatatcggt
    15421 accctgacat ccatctgctt ttcaaagttt cctccgattc tgcagtattt cagagagcac
    15481 catcccaggg ttcctattcg tattaccact gcatctccgg gagaactgat tgacatgatg
    15541 gagcataatc agctggatct ggtttacctg ttggatcgtt cacgctacag tgatcgatgg
    15601 gaaaaggtga tggaggtacc ggaaccggtg atgtttgtgg cttctccacg gtttcatttg
    15661 gccgggcaga aggagattta tctggagcag cttctgaaag agccttttct tctgacagag
    15721 agaaacgaaa actaccgcag ggagctggat cagtttctgg aatccagagg agtaacactg
    15781 actccgtttt tggaaatcag cagtacggaa tttattatac gtatggtgtg tgaaaacaga
    15841 ggaattacct atctgccgta ttttgccgtg gagaagaata tagaagaaaa aaatcttatg
    15901 gtacttaatg tgcctgattt taagcttcag atgtatcgtc agatctttta ttataaaaac
    15961 aaatggaaaa ctccggaaat ggaggaattt atccgtttag cccgtgaact gcggtagcga
    16021 aaatttcgct ccgcagtttt ttgtctataa aaataggttt aaaaacagga taacaattca
    16081 tatattttta aatcacaatg agataaaaat aagccttcca ttaaaaaatc acaaataatt
    16141 ttccaaaatc cccaaagcct attatgggaa aattatgcat attttatttt cataatgtga
    16201 aagttatcaa tttataacaa tttccatatt tgaaatggga atatcaggtg gctataatag
    16261 cgtcgaaatg aaaaagaaac tgttttggag gaaaggatgg tgagataagt tggatcagct
    16321 ggttgaatca ttgatggaag aattgaattg taacactgta tttacaattc ccatatttgg
    16381 aggaattcag gtctttgaat ctgtggtggt aacctggata atcatggcag ttctgatggg
    16441 ggcttccctg atcctgacca gacatttgaa ggtcaggaat ataagtacac gtcaggcagc
    16501 gctggaaagt ggcttttcat ttgtttacag cttcttttca gatctgctgg gagaagaggg
    16561 aaaagaatat attccctatc tgatcacagt agcggtttat attgcaattg caaacatgat
    16621 cggccttttg ggatttaaat ctcccacaaa ggatctgaat gtaactgcat cactggcggt
    16681 tatgagtatt attttagtag aggctgcagg aatccgcagt aaaggcgtaa agggctggat
    16741 cagaagtttt gcggagccaa tacccattgt tgcgccgatt aatgtgctgg agctttttat
    16801 ccgtccgctg tcactgtgca tgcgtttgtt tggaaatgta ttgggagcaa ttgtggtcat
    16861 ggctttaata aagtatcttg tgcctctggt gcttcccctt ccgttcagcg gatattttga
    16921 tatatttgat ggagtgatac aggcatacgt gtttgttttt cttacatcat tatatataaa
    16981 agaggcaatt gaataaacaa aaacaaataa aaggagattg aaaaaatgtc aggaattatc
    17041 gcaattggag cagcagtagc agttattaca ggaattggag caggaattgg aatcggtatg
    17101 gcaacttcta aagcagttga tgcaatcgca agacagcctg aggcagacgg taagatcaca
    17161 aaggctcttc tgctgggatg tgcccttgca gaggctactg cgatttatgg ttttgttatc
    17221 ggccttctga ttatcctgtt tttaaagtaa gttggaatag aaaggcattt tgaatatgtt
    17281 aagaataagc gtttggaata ttgcttttac gatcattaat attctggtgc tgtttttctt
    17341 catgaggcac ttcctgatga aacctgtcat ggaaattctg gaggaaagaa agaagctggt
    17401 ggaaaaggat ctggacgagg cagacagatc aaaaatccag gcagcagaac tcaaagagag
    17461 gtatgaggca tctcttgcag ctgctgaaaa agaggccgac tgtatcgtag caaaggccag
    17521 gcagcaggcg cagagtgaat atgaacgtat gatggaacag gcagatcagg atgccaggaa
    17581 aaaactgcag gcttctgaaa agaccatgga actggaacgg gaaaaggtat taaatgaatt
    17641 gagaacctct gtaacaggac ttgccatgac agctgccgca aggcttttga cagagaagaa
    17701 cagcccggaa tggaataaac ggcagtatga tgtatttctg gcaggtgaag gtgagaagaa
    17761 tgactgaaca gacacggatg tacggaaggg ttctttatga attgcatctt cctgaaaaaa
    17821 tggtatgtga gatggtaaag gttattcggg aaaatccact gttggttcag atcctgtcag
    17881 atccggtgct gccgtcattt aaaaaggaca gaataattga aaaagtgtgg cgggcctctg
    17941 agtttacgga aatagtacct tcctttctga aaaaggtatg tgcagcaggc tgtattggca
    18001 gtatggattc catactgtca gtatgggaac agtgcctgag ggatgaaaaa ggaattattc
    18061 aggcagaact tttttacgtt acaccgccag atgaggagga acttcagaaa atccgcagat
    18121 ttttgtgtgg aaaatatgga aaaaaagagg ttcagtttat actggcgtct gccccggaac
    18181 tgatcggagg ttttcttctt cgaacagaaa atgtggaata tgactatagt ctgaaaagcc
    18241 ggttagaaga actttttggt atataggaga ataggagtta tcattatgag tacaatcagt
    18301 tctcaggaag ttatctccat cgtaaagggc gagattgagg attacgatgc tcatgcaggc
    18361 agaattcgtg aaaccggatc tgtaatctgg gtgggagatg gaattgccat tgtatatgga
    18421 atcgatcacg ctatgtatgg agagattgtg atatttgaaa acggtgttcg cggtatggtt
    18481 caggacattc gtaaaaatga gattggctgt atcctttttg gaaaagatac aggaattatg
    18541 gaaggtacaa aggtaacaag gacaagaaaa cgggctggag ttcctgtggg aagcggattt
    18601 ttaggccgtg tcgtcaatgc gctcggtgat actatagacg gtcttggtcc tgtaaaagaa
    18661 gaggattatt atccggtaga gcgggaggct cccggaatag tggaaagaaa atctgtaagt
    18721 gttccaatgg aaacgggtat tcttgctatt gattccatgt tccctattgg ccgggggcag
    18781 cgagagctga ttatcggtga ccgtcagact gggaaaacat ccattgcact ggatactatt
    18841 atcaatcaga aaggaaagga tgtaatctgt atctatgttg ctataggaca gaaggcatct
    18901 acagttgcaa agctggttaa tactctgaa
//
LOCUS       DBMDUH010000037        18771 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_1769_length_18771_cov_5.458490, whole genome shotgun sequence.
ACCESSION   DBMDUH010000037 DBMDUH010000000
VERSION     DBMDUH010000037.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 18771)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 18771)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18771
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1769_length_18771_cov_5.458490"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="ACZPCG_04955"
     CDS             complement(<1..524)
                     /locus_tag="ACZPCG_04955"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007717859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HIR1284458.1"
                     /translation="MKSDIEIAQEAAMIPVKEVAARYGIKEDDLELYGKYKAKLTDEL
                     WNQIKDRPDGKLVLVTAINPTPAGEGKTTVTVGLGEAFGRMGKNSIIALREPSLGPCF
                     GIKGGAAGGGYAQVVPMEDLNLHFTGDFHAITSANNLLAALLDNHIQQGNSLGIDPRQ
                     ILWKRCEDMNDRALR"
     gene            complement(646..3789)
                     /locus_tag="ACZPCG_04960"
     CDS             complement(646..3789)
                     /locus_tag="ACZPCG_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HIR1284459.1"
                     /translation="MSDAVKKQQGSARKPGRPKGSTTKKNTGTTTAKRQSSTSRDKSR
                     PRRPAYEEEEDMGFMRAEVIIICSFAVAILLFLSNFRLCGVFGDVLRSVQLGLFGMIG
                     YLLPIMLFVGTCFHLSNQGNIHAAVKLAAVIGVFFTLCGLLQLMFGKAPEGASLMEYY
                     TVSSVNGAGGGLVGGALSGLLSFLLGRAGAYLVLVVLLIICMVCITGKSFVSFVQQGS
                     DRAYQYAREDMDRRRELHAQRAEERRLLKEEQRVRGVNLEATALGGRGDRTEEFYEEE
                     GLAEDAFEREFGADPVRKGEPAENEASPADEFRGRIQLPRNYDTAGTVGARQERTEGE
                     AGRLARELEDREKTSEARRECKNEPTPQSRTEQGDCGGAEEEEPIYETIRVQRDRRTL
                     DELDMGVGAPRPVSFEEDISGLQETVYRQEPVSAPAPAREPEKQERDEMPEQKAVHIA
                     PWDTEPEEETIHLDEGSEEPDCDGTSPWDPDPDSSPTSAPKPFEAVQPSMMAAPDTLY
                     QPEGTKRVVTASGKIIETETDLLQKKLEKKREAAEVQAAEVSVAQEIHKKIEEKKEYV
                     FPPTDLLKKPVHNAAFSENEFKATAIKLQQTLHNFGVGVTVTNISCGPSVTRYELLPE
                     QGVKVSKIVSLTDDIKLSLAAADIRIEAPIPGKSAVGIEVPNKENNIVYLRELFESEA
                     FRRHKSRLAFAVGKDIGGQVVVTDIAKMPHLLIAGATGSGKSVCINTLIMSIIYKADP
                     ADVKLIMVDPKVVELSVYNGIPHLLIPVVTDPKKASGALNWAVAEMTDRYKKFAECNV
                     RDLKGYNERVAKLDDIDDDKKPKKLPQIVIIIDELADLMMVAPGEVEDSICRLAQLAR
                     AAGIHLVIATQRPSVNVITGLIKANVPSRIAFSVSSGVDSRTIIDMNGAEKLLGKGDM
                     LFYPAGFPKPQRVQGAFVSDEEVSRVVEFLTEQGMVAEYSQEVVNKVASPSMDGGSSG
                     ADRDELFAQAGAFIIEKDKASIGMLQRAFKIGFNRAARIMDQLAEAGVVGEEEGTKPR
                     KILMSPEQFEEMMEQGY"
     gene            complement(3884..4822)
                     /locus_tag="ACZPCG_04965"
     CDS             complement(3884..4822)
                     /locus_tag="ACZPCG_04965"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002593496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HIR1284460.1"
                     /translation="MKYFDLHCDTIEELKKRGENFLNSTTQFTIQDQERFEATVQCMA
                     VWVPDSIRGRAAVEFTDSHLAYLETLVEMIPERAVLADSASELQKGVEEGKWVFIRTI
                     EGGAALGGDLEKIVHFAKKKIKMMTLTWNGENELGSGCAGTGGLTGFGKEAVKLMEEN
                     GIIVDCSHLNDAGFEDLLNITKRPFVVSHSNLRSCCEVPRNLREDQFVELVRRGGLCG
                     INLYSRFLSVPDQKGEGERELLFRHIYRMLELGGEDCIAWGTDLDGQITCDPSLGSPF
                     GAASFGTYLAERGIPEGAVKKFCFENAYRFFQSYMR"
     gene            complement(4880..5593)
                     /locus_tag="ACZPCG_04970"
     CDS             complement(4880..5593)
                     /locus_tag="ACZPCG_04970"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016287547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0051117 - ATPase binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP family protease"
                     /protein_id="HIR1284461.1"
                     /translation="MNRQEERQTEQEIREKEDRGLKEYGQMTLEENGKKRKIHLLSII
                     GEVEGHENSPGSSKSTRYDHVLPRLAQIEDDDSIDGMLVLLNTSGGDVDAGLAIAEMI
                     ASMSIPVVSLVLGGSHSIGVPLAVSSCYSFIVPSGTMMVHPVRMTGMVIGTERTYDYF
                     QMIQDRILTFVSEHSAIAYDKLHDLMHNTEMLTRDLGTVLVGKEAVDVGLIDAVGGIK
                     EALAKLYEMIDERKNPAKQ"
     gene            complement(5707..7371)
                     /locus_tag="ACZPCG_04975"
     CDS             complement(5707..7371)
                     /locus_tag="ACZPCG_04975"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002573336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HIR1284462.1"
                     /translation="MLEHKDYLSSLNKARRKRSQKPDPRLWILILAAVLAVGAIIFAV
                     KYMKKPESGEMAVRSVGEQLQTGEPQTDPAAAVAAESDEETQWEQQVEEAVGRFANLG
                     LIQVSGYVNIRETPGTDGTVLGTIADGGGCEVLGTEGEWTKIQSGGLEGYVSSQYLVT
                     GQEAKNLAEKYVKLRAVITMETEGEKLRVRETPDDSTSDNVIGAVSDGESFEVLSQED
                     GWIQIKDGYISDQFAQVSYALEEGRKLDEKTKAINQYENLVMSKVNGYLNVRSSPEDK
                     GNANIIGKMTSKAGGEILETLDGWYKVKSGPIVGYISSDPQYTATGREAKDIALQTAS
                     LKAVISTDVLNVRTEPSTDAKIWTQIVKDEKYPVIDDPETPDGWLKIDLDSVDEEDGG
                     VSDGAYISTRDNNVEVRYVINEAIKFKPAESGSGGSGSSGSGSRRSQIVNYAIQFVGN
                     PYVWGGTSLTNGVDCSGFTMKVMQKFGVSLPHYSGSQAKMGKKVTSGNMKPGDLIFYA
                     GSNGRINHVALYIGNGQVVHAASRRSGIKISTWNYRSPVAIRNMLD"
     gene            complement(7483..8280)
                     /locus_tag="ACZPCG_04980"
     CDS             complement(7483..8280)
                     /locus_tag="ACZPCG_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009662574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1284463.1"
                     /translation="MLEICHVNKTFNRGTVNEKQALKDLNLTLKDGDFVTIIGGNGAG
                     KSTTLNAIAGVWPVDEGKIMIDGRDVTKLPEYKRAAFLGRVFQDPMNGTVATMGIEEN
                     LALALRRGGRRTLKPGITGSERKLYRDLLLKLGLGLEERLTSKVGLLSGGQRQALTLL
                     MATLKKPKLLLLDEHTAALDPQTAKKVLELTSEIVEEQRLTALMVTHNMKDAIQIGNR
                     LIMMNDGHIIYDVRGEEKKNLTVDDLLKKFAEASGKEFANDRMMLAN"
     gene            complement(8280..9197)
                     /locus_tag="ACZPCG_04985"
     CDS             complement(8280..9197)
                     /locus_tag="ACZPCG_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1284464.1"
                     /translation="MSIMSLFNSLPGAVAQGLVWGIMAIGVYLTFRILDFADLTVDGS
                     LCTGGAVCIVMMLAGQNVWVALLCAALAGMAAGFCTGILHTFMGIPAILSGILTQLGL
                     YSINLKIMGKANQAINVDKYDLLVSLRFIRNVPLFKNTIFLVSVMILVLIGLLYWFFG
                     TELGCSIRATGGNDRMARAQGINTDFNRVLGLMVSNGLVALSGALLSQYQGFADINMG
                     RGAIVIGLAAVIIGEAIFGKVFHNFGFRLLGVALGSIVYYLVLQVVIWLGVDTDLLKL
                     LSAAVVAVFLAIPTWKGRYFKSAPKGGKS"
     gene            complement(9204..10220)
                     /locus_tag="ACZPCG_04990"
     CDS             complement(9204..10220)
                     /locus_tag="ACZPCG_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1284465.1"
                     /translation="MKKTAKIFSFVLAGAMAAAVTGCGSGTNETTAAAADTSKEAEGG
                     KEVYQVGICQLVQHVALDAATQGFKDALVETFGEDGVEFDEQNAQGDSNTCSTIINSF
                     VSEGVDLILANATASLQAAQAGTNEIPVLGTSVTEYGVALGISDFNGTVGGNISGTSD
                     LAPLDEQAAMIKELFPDKKNVGLLYCSAEPNSQYQVDTVKLALEELGYTCEYYAFSDS
                     NDLSTIATNAASASDVIYVPTDNTVASNTEIINNICQPAGVPVVAGEEGICAGCGVAT
                     LSISYYDLGTATGKMAAKILSGEANVSEMPIEYAPQFTKKYNKNICDALGVAVPDDYE
                     AIEE"
     gene            complement(10371..11771)
                     /locus_tag="ACZPCG_04995"
     CDS             complement(10371..11771)
                     /locus_tag="ACZPCG_04995"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1284466.1"
                     /translation="MRRAILKKFILVLLAALCINSVIFYLISSHMILKMAREDMTYTI
                     EMIDRLLDYEDESYLAVQIERMEEFTGADDTRVTLIHLDGTVVSDTDTSTEDLDNHLS
                     RKEVKEALLNGMGYAKRYSKTMGKHFLYVAFQSHRSDMILRISVPYSGFSEYLPMLLP
                     AALVSFLAALACAFVFTRRFVDSVTRPLQDIVSELQKVKGGNGELHFSPCPYQELNVI
                     AETTMEMSNNVKDYLNRIEKEKQIRQEFFSNASHELKTPITSIQGYAELMESGMIQDE
                     AMKLDFIRRIKKEASNMAGLIGDILMISRLEAKDAQVVLSDVRISPLLEEIAESLKPA
                     AAQSQVFIHIDCQPLCIKANVQQMKELLTNLVGNAVKYNHPGGQVWVTIREQDGQMVI
                     RVRDNGVGIPEESLDRIFERFYRVDKGRSRKQGGTGLGLSIVKHIVNFYHGSIHVASQ
                     LEKGTEFTVTIPLKWE"
     gene            complement(11768..12469)
                     /locus_tag="ACZPCG_05000"
     CDS             complement(11768..12469)
                     /locus_tag="ACZPCG_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009251484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1284467.1"
                     /translation="MEKIYIIEDDENIRNLLKIALEGFGFEAEGFETAEEGLERIRQE
                     CPSMAIFDWMLPGMDGVTAIRQIRQTEGISGLPIMLLTAKEKELDKVVGLDCGADDYM
                     VKPFGVLELSARIRSLLRRVRREEKKDILSYQGISINKKTREASSSGRVCELTLKEFE
                     LLCYLLENQARVVTRDELLNQIWGYEYDGETRTLDMHIRTLRQKLGEEGGSCIKTVRG
                     VGYRLVRSETKEEDR"
     gene            complement(12692..13870)
                     /locus_tag="ACZPCG_05005"
     CDS             complement(12692..13870)
                     /locus_tag="ACZPCG_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1284468.1"
                     /translation="MLQSFKLALKSIWGNKMRSFLTMLGIIIGVAAVIILVSLVNGYM
                     GTIVENFASMGVNQINVNVTNLTSRTVSPDEMYGFFEEHTDVFEALSPNVTISTTIKK
                     GNDSMTSTSVAGRSEQYLEIKDYELQQGRNITYSDILSRQKVCVIGAYVAQELFGSAE
                     KALGDTLKVGGYAYKVVGVAEIQDEDDMSDGGTDDFVWLPYTASISLARSANINSYTF
                     TVADTDDADEAKTLIENFLFEIFKDDDYYRVSAMSEMLDSLNEQIAMMSGMLGGIAGI
                     SLLVAGVGVMNIMLVSVTERTREIGIRKSLGADKGTIMQQFVIEAAVTSSLGGVIGIL
                     IGCGATAVVGAAIGISATPTFSAIVISFSVSVGIGLLFGYMPASRAANLNPIDALRSE
                     "
     gene            complement(13854..14588)
                     /locus_tag="ACZPCG_05010"
     CDS             complement(13854..14588)
                     /locus_tag="ACZPCG_05010"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1284469.1"
                     /translation="MNVNERLSEETHPLIELKHISKIYNMGGEEVRANDDVNISIQKG
                     EFVAIVGKSGSGKSTLMNIIGALDVPTTGEYYLGGEDVSKMGDKQLARIRNRMIGFIF
                     QQYNLLPKLNLLENVELPLLYAGTGAEERKKRAMASLEKVGLAEKWKNMPNQLSGGQQ
                     QRVSIARALAGNPSLILADEPTGALDSKTSRDVLNFLKQINEEGNTIVMITHDNSIAV
                     EAKRVIRIADGHKIFDGDVKDYAAIL"
     gene            complement(14609..16297)
                     /locus_tag="ACZPCG_05015"
     CDS             complement(14609..16297)
                     /locus_tag="ACZPCG_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR1284470.1"
                     /translation="MKKKKLWVRLLAALLAAAVLLTGLMIWKNKKTKAAAAARAQSLN
                     TAAVEKRTITSELSSSGTLAAKDTYSITSMVEGEVVAAAFEEGDQVQKDQVLYEIDSS
                     SMTTELTSAENTLTRSRNSYEDALEDYNQALSDYSGNTYKATESGYIKELYISAGDKV
                     SGNTKLADLYSDDVMEIRIPFLSGEAAQITPGSSAVLTLTDSGEQVEASVKAVANREE
                     ALSGGRLVQYVTFTVANPGGLTVSTRASARVGEWIGSEEGLFEASIDTSMEADLSSSV
                     EIEAMLVNEGDYVTKGTPVFRMTAKTADKLIQSYKDSLDKAQESVESAQSHLESTQDS
                     YDNYTITAPISGQVITKNYKVGDNITKNTSNTTVLAVIYDLSSLTFEMSIDELDIKEV
                     EVGQKVAVTADAYEGQTFSGTVTNVSLESTYSNGVSTYPVTVTMDEAGDLLPGMNVDG
                     IITLDQAEDVLSIPVDALMRGNQVYIKDDTVTEQQGPIPAGFKAVEVETGLISDSYVE
                     IKSGLSEGDTVYVAESSQSSDAVMMMPGGGMGGGPGGGMGGGPGGGMGGGPGGR"
     gene            complement(16310..17482)
                     /locus_tag="ACZPCG_05020"
     CDS             complement(16310..17482)
                     /locus_tag="ACZPCG_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007717739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HIR1284471.1"
                     /translation="MKGSKSYGRFICGSLAVSFAVSPFLSLTVLADNISSANFDLRRQ
                     VVKLTGIMEISSFRGQITRGDFAKMLVKASSYRENLPSSSVSVYADVPADHPNAVYIR
                     VAASQGWMSGYLGGNFKPEEPVLYKDAVKAVLTLLGYTDEDFTGEMASSRISKFNHLE
                     LNEDVSCQADEAITETDCMNIFYNLLKTKKKDSNEIYGTVLDCELNSDGEINPISILE
                     DERKGPILVHKNFSVAQSVPFDTEDANVFLNGAESTLAAVKASQQQSGFAVLYYNVKS
                     KTIWAYTTTGWDTKDDSGNNTYVLIKGEIKNIYYQSTDVMTPTSVRIEVDEANSDDDF
                     DTSEDVDSDGYLNISLDSSELQYLFSIYGNLEVGDDVVLVCSRSGSSYTAVDAIEY"
     gene            complement(17596..>18771)
                     /gene="cls"
                     /locus_tag="ACZPCG_05025"
     CDS             complement(17596..>18771)
                     /gene="cls"
                     /locus_tag="ACZPCG_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HIR1284472.1"
                     /translation="LDGRTAGLADYLERMAGAPVYKNTDIEYFPLGEDMFVRLIEELK
                     AAKHFIFMEFFIIDRGYMWDEILKVLEEKVKEGVEVRVMYDGMCCLMLLPFHYPRQLE
                     RKGIRCKMFSPIIPVLSTHQNYRDHRKIAVIDGRCAFTGGVNLADEYINRKVRFGHWK
                     DTALMVRGDAVKSFTMMFLQMWDVTEKKEEDYRRYLSAPAGEAAAGRMEKGYVIPYGD
                     SPFDKEQIGEQVYLDILSQAKEYVHIMTPYLILDDGMKDALCYAAKRGVEVIIIMPHI
                     PDKKYAYLLARTYYPELLKAGVRIFEYEPGFVHAKVFASDDEKATVGTINLDFRSLYL
                     HFECGTFLYRNPVIEKIEADYQETLKKCIPVTLETCYSYSWLGRKAGRLLRLIAPLM"
BASE COUNT         4436 a         5340 c         3855 g         5140 t
ORIGIN      
        1 cgcagcgcac ggtcattcat atcttcgcac cgcttccata aaatctgtct tggatcaatg
       61 ccgagggaat ttccctgctg gatatggtta tcaagaagag ctgccagaag gttattggca
      121 gaggtaatgg catggaaatc acctgtaaaa tgcagattta aatcctccat aggcacaacc
      181 tgggcatagc cgcctccagc agctcctccc ttgatgccaa agcaggggcc aagggacggc
      241 tctctcagcg ctataatgga attttttccc attcttccaa atgcttctcc caagcctact
      301 gtcacagttg tctttccctc tcctgccggt gttggattga tagccgtcac cagcaccagt
      361 ttaccatccg gacggtcctt gatctgattc catagctcat cggtcagctt ggccttatat
      421 tttccataca gttccagatc gtcctctttg attccatatc tggcggccac ctcttttacc
      481 ggtatcattg cagcttcctg tgcgatctca atatctgatt tcatttggct gatcctcctc
      541 tttttcggcg tcccgatcag gttatgtctt tctcctgccg gtgcctggta atttatattt
      601 tatgcccgga gtcattggga ttctcctttg gactcctgat gcattttaat acccctgttc
      661 catcatttcc tcaaactgtt ccggactcat aaggatcttt ctgggttttg tgccctcctc
      721 ttcgcccaca actcctgcct ctgccagctg atccatgatc cgggccgcgc ggttaaatcc
      781 gatcttaaag gcccgctgga gcataccgat ggaagccttg tccttttcaa taatgaaggc
      841 tccggcctgt gcaaacagtt catcccggtc tgcccctgag cttcctccat ccatggaagg
      901 agatgcaact ttatttacca cttcctggct gtactctgcc accataccct gctcggtaag
      961 aaattctacc acgcggctga cctcctcatc cgatacaaaa gcgccctgta cacgctgggg
     1021 ttttggaaaa cctgcgggat aaaagagcat atcgcccttt ccaaggagct tttctgcacc
     1081 gttcatgtcg ataatcgttc tggaatctac acctgaggac acggaaaatg caattctgga
     1141 aggcacgttt gccttgatca gtccggtgat aacattgacg gacggtctct gggttgcaat
     1201 taccagatgg atgcctgctg cacgggccag ctgggccaga cggcagatgg aatcctccac
     1261 ctctcccgga gccaccatca taagatcggc cagctcgtcg ataatgatca caatctgggg
     1321 cagcttcttt ggctttttgt catcatcgat atcatctaat ttcgcaaccc gttcattgta
     1381 gccctttaaa tcccggacat tgcactcggc aaacttctta taccggtctg tcatctcagc
     1441 caccgcccag tttaacgccc cggatgcttt cttcggatcg gtcacgaccg ggatcagcag
     1501 atgggggatt ccgttgtaga cgctcagctc taccaccttg ggatctacca tgatcagctt
     1561 cacatcggcc ggatccgcct tgtaaataat actcatgatc aatgtattga tacagacgga
     1621 cttaccggat ccagtagcgc ctgcgatcag aagatgaggc atctttgcga tatccgtaac
     1681 aaccacctga ccgccgatat cctttcccac agcaaaagcc agacgggact tgtggcggcg
     1741 gaaggcttcg gattcaaaga gctcacgcag ataaacaatg ttattttcct tattaggcac
     1801 ctcgatcccc actgcggatt tgccgggaat cggagcctcg atccggatat ctgccgctgc
     1861 aagactcagc ttgatatcat cagtcaaaga aacaatcttg ctgaccttta cgccctgttc
     1921 cggaaggagt tcataacggg taacagaagg accgcagctg atattcgtca cagtaacgcc
     1981 tacaccgaaa ttatgaaggg tctgctgaag cttgatcgct gtggccttaa attcattttc
     2041 agaaaaagcg gcattatgca caggcttttt cagcagatcc gtaggaggga atacatattc
     2101 ctttttttcc tctatctttt tatggatctc ctgagccacc gaaacctctg ctgcctgaac
     2161 ctctgccgcc tcccgtttct tttccagttt tttctgaaga agatccgttt ccgtttcgat
     2221 gatctttccg gaggcagtga caacacgttt tgtgccttcc ggctgataca gagtatcggg
     2281 agcggccatc atagacggct gtaccgcttc gaatggcttt ggcgcagaag tggggctgct
     2341 gtctggatcc ggatcccagg gagaggttcc gtcacagtcc ggctcctctg aaccttcatc
     2401 cagatggatg gtttcctctt caggttctgt atcccaggga gcaatatgta cagccttttg
     2461 ctccggcatt tcatctctct cctgcttctc cggctccctt gccggcgcgg gggcagatac
     2521 gggttcctgc cggtaaactg tttcctgtaa tccacttata tcctcttcaa agcttaccgg
     2581 tcttggagcg cctacaccca tatccagctc atccagagtt ctcctgtctc tctgaacccg
     2641 gatcgtttca tatatcggct cttcttcctc tgctcccccg cagtctccct gttctgtccg
     2701 gctctgaggc gtcggttcat ttttgcattc tcttctggct tcggatgttt tctcacggtc
     2761 ttccagctct ctggccagac gtcccgcttc tccttccgtc ctttcctgtc tagctcccac
     2821 agtgcctgct gtgtcatagt ttctgggaag ctggatcctt ccgcgaaatt catctgcagg
     2881 agacgcctca ttttctgcag gctcaccctt tcttacagga tccgctccaa attcccgttc
     2941 aaatgcatcc tctgcaaggc cttcttcctc ataaaattcc tctgtccgat cccctcttcc
     3001 ccccagagca gtcgcctcca gattcacacc gcgtacacgc tgctcctcct taagaagacg
     3061 tctttcttct gctctctgag catggagttc tctcctgcgg tccatatcct cccttgcata
     3121 ctgataggcc cggtcacttc cctgctgtac aaaggataca aaggattttc ccgtaataca
     3181 taccatacag atgataagga gcaccaccag taccagatat gctccggccc gtcccagaag
     3241 gaaactcaaa aggccggata atgctccgcc gaccagcccg cctcctgctc cattcacaga
     3301 ggataccgta taatactcca tcagagaagc gccctcaggc gcctttccaa acatcagctg
     3361 gagcaggccg cagagcgtga aaaacactcc tatgactgca gccagtttca ctgctgcgtg
     3421 gatattcccc tgattggaca ggtgaaaaca ggttcccaca aaaagcatga taggcagaag
     3481 atagccgatc atgccaaaaa gtcccagctg cacgctgcgc agcacatctc caaacactcc
     3541 gcataagcgg aaattgctta aaaacaaaag gattgccaca gcaaaagagc agatgatgat
     3601 cacttctgct ctcataaatc ccatatcctc ttcttcctca taagccgggc gtcttggccg
     3661 gcttttatct ctggatgtgc tgctctgcct ctttgcagta gttgtccccg tgttcttttt
     3721 tgtcgtactt cccttgggcc tccccggttt tctggcgctg ccctgctgtt tctttactgc
     3781 atcagacaca aatacaagcc tcccattcct cgttcctata ttaagtcttg acttacatta
     3841 cttatagtat acaaaaattt aatgggaatt gcaacgcagt tttttaccgc atataagatt
     3901 ggaaaaaacg gtacgcattt tcaaagcaga attttttcac agccccttcg ggaatccccc
     3961 gctccgcaag atatgttcca aaggaagccg ctccaaacgg cgagccaagg gaagggtcgc
     4021 aggtgatctg tccgtccaga tctgttcccc aggcgataca gtcctctcct cccagctcca
     4081 gcatcctgta tatatggcga aacagcagtt cgcgctcccc ctctcccttc tgatccggaa
     4141 cagaaaggaa acgggaatat aaattaatac cgcagaggcc gccccggcgt acaagctcca
     4201 caaactgatc ctctcttaaa tttcttggca cctcacagca ggaacgcagg ttggaatggg
     4261 agacaacaaa tggccttttc gttatgttca aaagatcctc aaagcctgca tcgtttaaat
     4321 gggagcaatc cacaatgatt ccattttctt ccataagctt cacggcctct tttccaaatc
     4381 ccgtaagccc tccggttccg gcacagccgc ttcccagctc attttcccca ttccaggtaa
     4441 gggtcatcat cttaatcttc tttttggcaa aatggacaat cttttccaga tctcctccca
     4501 gggctgctcc cccttctata gtccggataa atacccattt cccttcctct acccctttct
     4561 gcagctcaga agcggaatct gccagaactg cccgttccgg gatcatttcc acaagcgttt
     4621 ccagataggc cagatggctg tctgtaaatt ctactgccgc tcttcctcga atggaatccg
     4681 gtacccagac ggccatacac tggacagtcg cctcaaaccg ttcctgatcc tgtatggtaa
     4741 actgagtggt gctgtttaaa aaattctccc cgcgtttttt caattcttca atggtatcgc
     4801 agtgaaggtc aaaatacttc atgactgttc cccctgtctt atcttatgtg tatatcatac
     4861 gatacttctg ctctttcttc tactgttttg cgggattttt tctctcgtcg atcatctcat
     4921 acagtttcgc taatgcctcc tttatcccgc cgactgcatc gatcagcccc acgtctactg
     4981 cctcttttcc cacaagaacg gttcctagat ccctggtaag catctctgta ttgtgcatca
     5041 gatcatggag cttgtcatag gcaatggcgg aatgctccga tacaaaggtt aaaatacgat
     5101 cctgtatcat ctgaaaatag tcataggtcc gctcagtgcc gatcaccatg cccgtcatgc
     5161 gcaccggatg aaccatcatg gtccctgaag gtacaatgaa ggaatagcag gaggatacgg
     5221 ccaatggtac gccaatggaa tggctgccgc ccaaaaccag cgatactacc ggaatgctca
     5281 tagatgcgat catctcagcg atggccagtc ccgcatccac gtctcctccc gatgtattta
     5341 aaagaaccag cattccgtca atgctgtcgt catcttcgat ctgggccagt ctgggaagca
     5401 catgatcgta tcgggtgctt ttggaagaac cggggctgtt ttcgtgtcct tccacctctc
     5461 ctataataga gagcagatga atttttctct tttttccatt ttcttccaga gtcatctggc
     5521 catactcttt taaccccctg tcctcttttt cacggatttc ctgctctgtc tgccgttctt
     5581 cctgtctgtt catataaaaa agaccatcct ttccaaatcg ggccttacca caaagggata
     5641 ggggatacct tatataaggc acccggccca tattctttca ggatagtctt tccttttttc
     5701 tttcttttaa tccagcatgt tgcggattgc taccggagag cggtaattcc aggtagaaat
     5761 cttgatgccg ctgcgcctgc ttgccgcatg aaccacctgt ccgtttccaa tatacagagc
     5821 cacatggttg atacggccat tgctgcctgc atagaatatc agatcgcccg gcttcatatt
     5881 tccagaagtg accttctttc ccattttggc ctgcgagccg gaatagtgag gaagcgatac
     5941 tccgaatttc tgcattacct tcatggtaaa gccggagcag tctacgccgt ttgtcaggct
     6001 ggtaccgccc cagacataag gatttcccac aaactgaatc gcatagttca ctatctgaga
     6061 gcggcgtgaa ccggagcctg agcttcctga acctccggag ccgctttcag caggtttaaa
     6121 cttaatagcc tcattgatca cataacggac ttccacatta ttatctcttg tagaaatata
     6181 ggctccgtct gatacgcccc catcttcttc atccacggaa tcaagatcga tcttcagcca
     6241 gccgtccggc gtttcaggat cgtcgatcac aggatatttt tcatccttta cgatctgggt
     6301 ccagatcttg gcgtccgtac tgggttctgt gcggacgttt aacacatccg tactgatcac
     6361 cgctttcagg gatgcggtct gaagggcaat atcctttgcc tccctgcccg tagctgtgta
     6421 ctggggatca gaggaaatat aacctacgat agggccggat ttcaccttat accagccgtc
     6481 cagagtttcc aggatctctc ctcccgcctt gctggtcatc tttccgataa tattggcatt
     6541 tcctttatcc tccggtgaac tgcgcacatt cagataaccg ttcaccttgg acatcaccag
     6601 attttcatac tgattaatgg cctttgtttt ttcatccagt ttgcgtcctt cctccagagc
     6661 atagcttacc tgggcaaact gatcggaaat atatccgtcc ttaatctgga tccatccgtc
     6721 ctcttggcta agcacctcaa aactttcccc gtctgatacg gctcctatga cattgtcgct
     6781 ggtggaatcg tccggagttt cccgcacccg cagtttttcg ccttctgtct ccatagtaat
     6841 cacagctctg agctttacat atttttccgc cagatttttt gcctcctggc cggttacaag
     6901 atactggctg ctcacatagc cctctaaacc tcctgactgt atctttgtcc attcgccctc
     6961 tgttcccaga acctcgcatc cgcctccgtc tgcgatagta cctaaaaccg taccgtctgt
     7021 tcccggagtc tcacggatat ttacatagcc cgacacctgg atcagaccca gattggcaaa
     7081 acggcccaca gcttcctcca cctgctgttc ccactgtgtc tcttcatcgc tctctgcagc
     7141 cacagccgct gccgggtctg tctgcggctc tcccgtctgg agctgctcac ccaccgaccg
     7201 aaccgccatt tccccagatt ccggcttttt catatatttc accgcaaaaa taatagctcc
     7261 cacagccagg acagctgcca ggatcagaat ccacagcctg ggatccggct tctggctgcg
     7321 cttcctccta gccttattca ggctggataa ataatcctta tgctcaagca tattttaatc
     7381 gtctccatct caaatttgat tcctgctttg cagtgcaaaa cagcctgtcc tttttattat
     7441 accataaagg acaggctgga tgggtgaatt ttttatgaat ttttaatttg ccagcatcat
     7501 acggtcattt gcaaattctt tgccgctggc ctcggcaaat ttctttaaca ggtcatccac
     7561 cgtgaggttc tttttctcct cccccctcac atcatagata atatggccat cattcatcat
     7621 gatcaggcgg ttgccgatct ggatggcatc cttcatatta tgagttacca tcagggcagt
     7681 cagccgctgc tcctctacga tttcactggt aagctccaga accttcttcg ccgtctgagg
     7741 atccagggca gcagtgtgtt catccagcaa aagcagcttt ggctttttta aagttgccat
     7801 cagaagggtt aaggcctgtc tctgtccgcc ggacaaaagt cccaccttag aggtaaggcg
     7861 ttcctccagc cccagtccca gctttaagag cagatcccgg tacagctttc tttcactgcc
     7921 tgtaataccg ggctttaatg tgcggcgccc tcctcttctt aaagccagag ccagattttc
     7981 ctcaattccc atagtagcca cggttccatt catgggatcc tggaatacac ggcccaaaaa
     8041 ggcggctctt ttatattccg gaagctttgt cacatccctt ccatctatca tgatctttcc
     8101 ctcgtccaca ggccacacgc ctgcaatcgc attcagggtc gtagactttc cggcgccgtt
     8161 tccgccgatg atcgtaacaa aatctccatc ctttaaggtg aggttcaaat ctttcaaggc
     8221 ctgcttttca tttaccgtgc ccctgttaaa tgttttattc acatgacaga tttccagcat
     8281 cagctcttac ctccctttgg cgcactctta aaatatcttc ccttccaggt ggggatcgcc
     8341 aggaaaacag ctaccacagc agcggaaaga agcttcagta aatccgtatc aactcccagc
     8401 cagatcacaa cctggagcac cagataatat acaatagagc ccaaagccac acccagaagg
     8461 cggaagccaa agttgtggaa aacttttcca aaaatcgctt ctccgataat cacagcggcc
     8521 agtccgatga caatggctcc gcggcccata ttgatatctg caaagccctg atactgggat
     8581 aaaagcgcgc cggaaagtgc gaccagtcca ttggaaacca tcagccccag cacacggtta
     8641 aaatcggtgt tgatcccctg tgctcttgcc atgcggtcat tgccgccggt ggcacggatg
     8701 gaacagccaa gctccgtgcc gaaaaaccag taaagcagac cgatcagtac caggatcatc
     8761 acagacacca gaaaaatggt atttttaaac agtggtacat tgcggataaa acgcagggaa
     8821 accagcaggt catacttgtc tacattgatg gcctggttgg cttttcccat aatttttaag
     8881 ttaatggaat acaggcccag ctgggtcagg ataccggaaa gaatagccgg gattcccata
     8941 aaggtatgaa ggatacctgt gcagaatcct gctgccatcc ctgccagagc ggcacataaa
     9001 agagccaccc agacattctg tccggcaagc atcatcacga tgcagacagc gccccccgta
     9061 caaagggagc cgtctacggt cagatccgca aaatccagga tgcggaaggt aaggtatacg
     9121 ccaatcgcca ttatgcccca cactagtccc tgggccacag cgcctggaag tgaattaaat
     9181 aagctcataa tactcatgat cggttattcc tcgattgctt cataatcatc cggcacggca
     9241 actcccagtg catcgcagat gtttttgttg tacttctttg taaactgagg cgcatactcg
     9301 atgggcatct cagatacatt ggcttcgccg gaaaggatct ttgcagccat ctttccggtt
     9361 gccgtaccca gatcatagta gctgatggat aaggtggcaa cgccgcagcc cgcgcagatg
     9421 ccctcctcgc cagctaccac gggaacgcct gccggctggc agatattatt gatgatctct
     9481 gtgttggaag caacggtatt atccgtagga acatagatca catcgcttgc agaagctgca
     9541 ttagtagcaa tggtggataa atcattggaa tcggaaaatg cataatactc acatgtataa
     9601 cccagctctt ccagagccag ctttaccgta tctacttggt actgagagtt aggctctgca
     9661 gagcagtaga gcagacctac atttttctta tccgggaaca gctctttgat catggccgcc
     9721 tgctcgtcca gaggagccag atccgatgtt ccggaaatat ttcctcctac ggttccgtta
     9781 aaatcggaaa ttcccagggc cacaccgtat tctgttacag aagttcccag cacaggaatc
     9841 tcgttggtgc ctgcctgagc ggcctgaaga gatgcggttg catttgccag gatcagatcc
     9901 acaccctctg atacaaagct gttgatgatc gtggaacagg tgttggaatc accctgggca
     9961 ttctgctcat caaactccac accgtcttct ccaaaggtct ccaccagagc atccttaaag
    10021 ccctgagtcg ccgcatccag ggcaacgtgc tgtaccagct ggcagatacc tacctggtat
    10081 acctccttac ccccctctgc ttcctttgaa gtgtccgcag cagctgccgt tgtttcattt
    10141 gtgcctgatc cacatcctgt tacagccgca gccatcgctc cggccagtac gaaggaaaaa
    10201 atttttgccg tctttttcat aacatcgctc ctcgttttta tccaaaattt cgtccgtttc
    10261 tttcgaactt tttttatcat actacaaaaa aaagggcgcg tcaattttat tgctttaaat
    10321 ttttgtgttt ttaacgcatt ggcgctttta tgtatcaaag tatagcccaa ttattcccat
    10381 ttcaatggaa ttgtaaccgt aaattccgtt cccttttcca gctgagaagc cacatggata
    10441 gaaccgtgat aaaaatttac gatatgcttt acgattgaaa gtcccagtcc cgtacctccc
    10501 tgtttccggc tgcgtccctt atccacccgg taaaaacgtt caaaaatacg atccaggctt
    10561 tcctccggta tccccacgcc attatcccgg acacggatca ccatctgtcc gtcctgttct
    10621 ctgatcgtca cccaaacctg tcctcccgga tgattatact taaccgcatt tccgaccaga
    10681 tttgtaagaa gctccttcat ctgctgcaca ttggccttta tgcacagcgg ctggcaatct
    10741 atatggataa agacctggct ctgggcggct gcgggcttta agctttctgc gatctcctcc
    10801 aaaagagggc tgatccgcac atcggaaagc accacctggg catcctttgc ctccagccgg
    10861 gaaatcatca gaatatcccc gatcagccct gccatattgg aagcctcctt tttgatccgc
    10921 cggataaaat ccagcttcat ggcttcatcc tggatcatcc cgctctccat cagctctgcg
    10981 taaccctgga tcgaggtaat aggagtttta agctcatggg aggcattgct gaaaaactcc
    11041 tgacggatct gcttttcttt ctctatacgg ttcaaataat cttttacatt atttgacatt
    11101 tccatggtcg tctctgcgat cacattcagc tcctgatacg gacagggcga aaaatggagc
    11161 tccccgtttc ctcccttcac cttctgaagc tccgaaacaa tatcctgcaa gggtcttgta
    11221 acggaatcca caaaccgcct ggtaaataca aatgcacagg ctaaagcggc aagaaagctc
    11281 acaagggcag ccggcagcag catgggcaga tattcgctga atcctgagta gggaacggaa
    11341 atgcgaagga tcatatctga tctgtgtgac tggaaggcaa cgtaaagaaa atgctttccc
    11401 atggttttag aataccgctt tgcatatccc attccattca gcagagcctc ttttacctct
    11461 ttgcggctta aatgattatc cagatcttcc gtagaagtat ctgtgtcaga tacaaccgtg
    11521 ccatctaaat ggatcagtgt aacccgcgta tcatccgcgc ctgtaaactc ctccatacgc
    11581 tcgatctgga cagccagata cgattcatcc tcataatcga gcagacggtc aatcatttct
    11641 atagtatagg tcatatcctc tctggccatc tttaagatca tatgactgct gataagataa
    11701 aagatgacgc tgttaataca cagcgccgcc agaagtacca gtataaattt tttcagaata
    11761 gcccttctca tcgatcctcc tcctttgtct ccgagcgcac caggcggtag ccaacgccac
    11821 ggacggtctt gatgcagctt ccgccctctt ctcccagctt ctggcgcagg gtacggatgt
    11881 gcatatccag tgtccttgtc tctccgtcat actcatagcc ccagatctgg tttaaaagtt
    11941 catccctggt tactactctg gcctgattct ccaaaagata acataaaagc tcaaactcct
    12001 ttaaagtaag ctcgcagaca cggccgctgc tggaagcctc tcttgtcttt ttattgatac
    12061 tgatcccctg ataggacagt atatcctttt tttcttcccg ccttaccctg cgcaggagac
    12121 tgcggatccg ggcagaaagc tccagaacgc caaagggctt taccatataa tcatctgctc
    12181 cgcagtccag ccccactact ttatccagct ccttttcctt tgctgtcaaa agcatgatcg
    12241 gaaggccgct tatcccctcg gtctgacgga tctgccggat ggccgtcacc ccgtccattc
    12301 ccgggagcat ccagtcgaag atcgccatgg acgggcattc ctgccggata cgctccagcc
    12361 cttcttctgc tgtttcaaac ccctctgcct caaagccaaa cccctccagt gcgatcttta
    12421 acagatttct gatattttcg tcatcctcta tgatgtaaat tttttccata aatgctgatt
    12481 cctgcccttt ccctgttatt aaatttacat attatgcttt ttaaagcctc atgtaaacag
    12541 cataccaaac agaagtaaaa tccgaaccaa tgaaatgtaa aatctgcgta aattttcata
    12601 tatcatccta ttcagtttat actacgtcaa aaaagatgtc aatgaaaaat ccccatcgca
    12661 ccatatacgg cagatgggga atgtaaactc cttattcact tcgcagcgca tcaatcggat
    12721 ttaaattagc cgcacggctg gctggcatat aaccaaaaag caggccgatc cccacggata
    12781 cgctaaatga aattacaatg gcagaaaatg taggtgtagc cgaaatgcca atggctgccc
    12841 ctactactgc agtagccccg cagccaatga gaattccgat cactccgccc agagaactgg
    12901 taactgccgc ttcgatcaca aactgctgca taatggttcc cttgtccgcc ccaagagatt
    12961 ttcggattcc aatttccctt gtccgctcag tcacagacac cagcatgatg ttcataacgc
    13021 ccactccggc taccagaagg gaaatcccgg cgattcctcc cagcataccg ctcatcatgg
    13081 caatctgttc gttcagggaa tccagcattt cactcatggc agatacccta tagtaatcgt
    13141 catccttaaa aatctcaaaa aggaaattct caatgagcgt ctttgcctca tccgcatcat
    13201 ccgtatctgc cactgtaaag gtgtagctgt taatattagc gcttctcgcc agactaatgg
    13261 aagccgtata tggaagccat acaaaatcat ccgtgcctcc gtcgctcata tcgtcctcat
    13321 cctgaatttc tgctacgccc actaccttat aggcatagcc ccctaccttc agcgtatccc
    13381 caagagcctt ttcagcactg ccaaacagct cctgggccac atacgctccg atcacgcaga
    13441 ccttctgacg ggaaagaata tccgaatagg tgatatttct tccctgctgc agctcatagt
    13501 ccttaatctc cagatactgc tcgctgcgtc ctgccacact ggtagaagtc atggaatcat
    13561 ttcccttttt tatcgtcgtg gaaatggtca cgttggggct taaagcctca aatacgtctg
    13621 tgtgctcttc aaaaaatcca tacatttcat ctggacttac agttcgggat gtcagattgg
    13681 ttacgtttac attgatctgg ttcactccca tgctggcaaa attttctacg atcgtcccca
    13741 tgtatccatt taccaggctt acgagaatga tcaccgcagc cacaccgata ataatcccca
    13801 gcatggtaag aaaagaacgc atcttattgc cccatatgct tttaagagcc agtttaaagg
    13861 attgcagcat aatcttttac atctccgtca aaaattttat gcccgtctgc aatgcggatc
    13921 acccgctttg cctccacagc aatggaattg tcatgggtaa tcataacaat ggtatttccc
    13981 tcctcattga tctgctttaa aaaattaagc acatcacggc tggttttgga gtccagcgct
    14041 cctgtgggtt catcagccag gatcagggat ggatttcctg ccagcgccct ggcaatagat
    14101 accctctgct gctggcctcc tgaaagctga ttaggcatat ttttccattt ttccgcaagc
    14161 cccacctttt caagggaagc catggcccgt ttttttcgtt cctctgctcc tgttcccgca
    14221 taaagaagag gaagctctac gttttccaga agattaagtt tgggaaggag attatactgc
    14281 tggaagataa aaccgatcat acggttgcgg atcctggcca gctgcttatc tcccatttta
    14341 cttacatcct caccgcccag ataatactct cctgtggtcg gcacgtccag agcgccgata
    14401 atattcatca gagtggattt tccgcttcct gattttccta caatagccac aaattcaccc
    14461 ttttggatag aaatatttac atcgtcattc gctctgactt cttctcctcc catattgtaa
    14521 atctttgaaa tatgcttcaa ctcgatcagt ggatgtgttt cctctgacag ccgttcattt
    14581 acgttcataa gctttttcac cctttctgtc agcggccgcc gggaccgcct cccattccac
    14641 cccctgggcc gcctcccatt ccgcctcctg ggccgcctcc cattccgcct ccgggcatca
    14701 tcatcacagc atcgctgctc tggctggatt ccgccacata aaccgtatct ccttcagaaa
    14761 gcccgctttt gatttccaca tagctgtcgc ttataaggcc tgtttctacc tccacggctt
    14821 taaagcctgc ggggatcggt ccctgctgct cagtaactgt atcgtccttt atgtagacct
    14881 gatttcccct cataagcgca tctacgggaa ttgaaagcac atcctctgcc tgatccaggg
    14941 taataatacc gtccacattc atacccggaa gaagatctcc tgcttcatcc atggttaccg
    15001 ttaccggata ggtgctgact ccgtttgaat aggtactctc caggcttacg tttgtaaccg
    15061 tacctgaaaa ggtctgcccc tcataggcat cggccgtcac agccactttc tggcccactt
    15121 ctacttcttt gatatccagt tcatcaatgg acatttcaaa ggtaagactg gacaggtcat
    15181 agatcactgc aagaacggtg gtattactgg tgttctttgt aatattatcc cccaccttat
    15241 aattctttgt gatgacctgg ccggaaatgg gagctgtgat cgtataattg tcatagctgt
    15301 cctgagtgct ctccaaatgg ctctgggctg actccacgct ctcctgggcc ttatccagac
    15361 tgtccttgta gctctggatc agcttatccg ccgtttttgc cgtcatgcgg aaaacaggtg
    15421 ttccctttgt aacgtaatcg ccttcattta ccagcatcgc ctctatttct acgctggatg
    15481 aaaggtctgc ctccatagag gtatcaatgg aagcctcaaa aagcccttcc tcacttccga
    15541 tccactcgcc tacccgggca gacgctctgg tagaaacagt caacccacca ggatttgcca
    15601 ctgtaaaggt cacatactgt accagacgcc ctccggaaag agcttcctct cggttggcaa
    15661 ctgccttaac cgacgcttcc acctgttctc ctgaatcagt cagcgtaagg acagcggaac
    15721 ttcccggggt aatctgggct gcttcgccgg aaaggaaggg gatccgtatc tccatcacat
    15781 catcactgta cagatccgcc agctttgtat ttccgcttac cttatcgcct gcactgatat
    15841 aaagttcctt gatatatccc gattctgtgg ccttatatgt atttccgctg taatcgctta
    15901 aggcctgatt ataatcctcc agcgcatcct cataggaatt ccgggaccgg gtcagggtat
    15961 tttcagcgct ggtaagctcc gttgtcatgc tggaactgtc gatctcataa agcacctgat
    16021 ccttttgcac ctgatctccc tcttcaaaag cggcggcaac cacctcgccc tcaaccatgg
    16081 acgtgatgct gtatgtatcc tttgccgcca gagtaccgga ggaggatagt tcagaggtaa
    16141 tggttctctt ttccacggct gccgtattaa ggctttgggc ccgggctgca gccgcagcct
    16201 ttgttttttt atttttccaa atcataaggc cggtaagaag tactgccgct gccagaagag
    16261 ccgccagcag acgaacccat aattttttct ttttcatctc gtttctccct taatattcaa
    16321 tcgcatccac tgccgtatag ctgcttccgc ttctgctgca caccagcacc acatcgtccc
    16381 ccacttccag atttccatag atggaaaaca gatactgaag ttccgaagag tccagactga
    16441 tattcagata gccgtcggaa tccacatcct cgctggtatc aaaatcatcg tctgaatttg
    16501 cctcatccac ctcgatgcgc accgaggtcg gcgtcatcac atctgtggac tgatagtaga
    16561 tatttttgat ctctcccttg atgagcacat aggtgttgtt gcccgaatcg tcctttgtat
    16621 cccagccggt ggtcgtatac gcccagatgg tttttgactt cacattgtaa tacagtactg
    16681 caaaaccaga ctgctgctgg gaagccttta ccgccgccag ggtgctttcc gcgccgttta
    16741 aaaacacatt ggcatcctcc gtatcaaagg gtacagactg ggccacgctg aaattcttat
    16801 ggaccaggat ggggcctttg cgctcatcct ccagtatgga aatgggattg atctccccat
    16861 ctgaattcag ctcgcagtcc agcacggttc cgtatatctc gttgctgtcc ttcttttttg
    16921 ttttcagcag attataaaaa atattcatgc agtccgtttc cgtaatcgct tcatccgcct
    16981 gacagctcac atcttcattc agttccagat gattaaattt cgaaatcctg gagctggcca
    17041 tctctcctgt aaaatcctca tctgtatagc ctaacagcgt taaaacggcc ttcactgcgt
    17101 ccttatataa aacaggctcc tccggtttga aattgccgcc cagatatccg ctcatccagc
    17161 cctgagaagc tgccacacgg atatagaccg cgtttggatg gtctgccggc acatccgcat
    17221 ataccgacac gctggaagaa ggaagatttt cccggtagct ggacgccttt accagcatct
    17281 tcgcaaaatc tcctcttgtg atctgccccc ggaagctgga aatctccatg attcccgtaa
    17341 gctttactac ctgacggcgc aggtcaaaat tggcgctgga aatattgtct gcaagcactg
    17401 taagagacaa aaagggcgaa acggcaaacg aaacagccag agaaccacag ataaagcggc
    17461 cataggattt ggaacctttc atacataaac tcctctaagt aattatacat atcaatgatt
    17521 aactgtcact aatgtaccat ccgtttgtga cgattttgtg ttctggctgt gtattttttg
    17581 ataaacaaaa agcgcctaca taagaggcgc gatcagacgc agcagccttc ctgccttgcg
    17641 gcccagccag ctatagctat aacaggtttc aagggtgacc ggaatacatt tttttagtgt
    17701 ttcctgataa tccgcttcga ttttttcaat aaccggattg cggtataaaa aagtgccgca
    17761 ctcaaaatgc aggtacagac tgcgaaaatc caggttgatg gttcccaccg tggctttctc
    17821 atcatcactg gcaaaaacct tcgcgtggac aaagcccggt tcatattcaa aaatccggac
    17881 tcctgccttt aaaagctccg gataataggt tctggccagc agataggcat atttcttatc
    17941 cggaatatga ggcataatga taataacctc cactccccgt tttgccgcat agcacagggc
    18001 gtccttcatg ccgtcatcca ggatcagata cggcgtcata atgtggacat attcctttgc
    18061 ctggctgagt atgtccagat agacctgctc gccgatctgt tccttgtcaa aggggctgtc
    18121 cccatagggg atcacatagc ctttctccat ccgtccggct gcggcttctc ctgccggagc
    18181 agaaagatat ctcctgtaat cctcttcctt tttctctgtc acatcccaca tctgaagaaa
    18241 catcatggta aagctcttta ccgcatctcc ccttaccatc agagccgtat ccttccagtg
    18301 gccaaagcgc actttgcggt taatatattc atctgccaga tttactcctc cggtaaaggc
    18361 acagcggcca tcaatgactg caatctttcg atgatccctg taattctgat gggtcgaaag
    18421 caccgggata atgggagaaa acattttaca gcggatccct ttccgttcca gctgcctggg
    18481 atagtgaaac ggaagaagca tcagacagca cattccgtca tacatcaccc ggacttctac
    18541 tccttccttt accttttcct ccagaacctt taagatctca tcccacatat atcccctgtc
    18601 aatgataaaa aactccataa aaatgaaatg ttttgccgcc ttcagctctt ctatcaaccg
    18661 tacgaacata tcctctccca ggggaaaata ctcgatgtcc gtatttttat ataccggcgc
    18721 tccagccata cgttccaggt aatccgccag tccggccgta cggccgtcca g
//
LOCUS       DBMDUH010000038        17889 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_1855_length_17889_cov_7.620924, whole genome shotgun sequence.
ACCESSION   DBMDUH010000038 DBMDUH010000000
VERSION     DBMDUH010000038.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 17889)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 17889)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17889
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1855_length_17889_cov_7.620924"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /gene="recA"
                     /locus_tag="ACZPCG_05030"
     CDS             <1..855
                     /gene="recA"
                     /locus_tag="ACZPCG_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006782980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HIR1284473.1"
                     /translation="GIAGFIDAEHALDPVYARNIGVDIDNLYISQPDNGEQALEITET
                     MVRSGAVDIVIVDSVAALVPKAEIDGEMGDSHVGLQARLMSQALRKLTAIISKSNCVV
                     IFINQLREKVGVMFGNPEVTTGGRALKFYASVRMDVRRIETLKQGGEVVGNRVRVKVV
                     KNKIAPPFKEAEFDIMFGKGISKTGDILDLAVKENIVEKSGAWFAYGGSKIGQGRENA
                     KQYLAEHPAICAEIEAQVRVRYQLPGAEEAEAEARKAAQAENVRPASSQSEAKEDPAA
                     EPAADEEA"
     gene            852..1469
                     /locus_tag="ACZPCG_05035"
     CDS             852..1469
                     /locus_tag="ACZPCG_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HIR1284474.1"
                     /translation="MRVTAVTPVDKRKCKVFLEEEDFAFPLYRGEAERFHIQEGSELP
                     ETVYLRIVEEILVPRAKERALYLLQAQGRTGAQMETKLSGDGYPKEVINRVMEFLKEY
                     RLIDDRVYTENFIRMNQEKKSRRQIAFELQQKGVDRQDISRILEGSAQDDLAAARNLL
                     KKRLRTADLSDLKEKNRLGAYLGRRGFSYDVIRRVMQEVQSGQEE"
     gene            1608..3167
                     /gene="rny"
                     /locus_tag="ACZPCG_05040"
     CDS             1608..3167
                     /gene="rny"
                     /locus_tag="ACZPCG_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HIR1284475.1"
                     /translation="MLQIVVTLMIGAAIAAVITWFIAHSAGRSYERKQYELKVGSAEE
                     KSREIIDEALKTAETKKREALLEAKEESLKTKNELEKETKERRAELQRYEKRVLSKEE
                     NVEKKADAMEKKEADLIRRENILGKRTAEVEAQYEQGIQELERISGLTSEQAKDYLLK
                     SVEEDVKHDTAKLIKELENKAKEEADKKARNLVVTAIQRCAADHVAETTVSVVQLPND
                     EMKGRIIGREGRNIRTLETLTGVELIIDDTPEAVVLSGFDPIRREVARIALERLIVDG
                     RIHPARIEEMVEKARREVENNMREEGEAACLEVGIHGIHPELVKLLGRMKFRTSYGQN
                     ALKHSIEVAQLSGLLASELGVDVRLAKRAGLLHDIGKSVDHDMEGTHVQLGADLCRKY
                     KESAVVLNAVESHHGDVEPTSLISCIVQAADTISAARPGARRETLETYTNRLKQLEDI
                     TNSFKGVDKSFAIQAGREVRIMVVPEQISDDDMVLLAREVSKRIESELEYPGQIKVNV
                     IRESRVTDYAK"
     gene            3343..4338
                     /locus_tag="ACZPCG_05045"
     CDS             3343..4338
                     /locus_tag="ACZPCG_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284476.1"
                     /translation="MIHLKKRLAFGLAAGLLAACITVPVYGASRKKITSISLTIKADI
                     EADTDYGDETIEIESSSSRYNVDGYEVMNEGFGWTEDTVPEIRIMLTANDDYYFTSLG
                     TDKITLKGGAEFKKATRQDSSETLLLDVTLPSMQNSLKDMEGVTLSQEGIASWPVIGT
                     AGSYEVRVYRGDKIVGASLTTETNSINCRERMLKPNESYMVKVRAVNKFDPTIKGEWV
                     SSNTIYVNGGQAAAFRENPDGAGVEGGSGAAGEWKQTEDGRWWYDQGDGTYPAGGWLE
                     IGHKWYFFDAEGYMQTGWIPWNEKEYYCSENGDMLTNCITPDHYLVGEDGAKIAQ"
     gene            4457..6580
                     /locus_tag="ACZPCG_05050"
     CDS             4457..6580
                     /locus_tag="ACZPCG_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HIR1284477.1"
                     /translation="MDKWKNWEQKLIEAVDTEYSYRLAKRMEEPKTNPVLGYRTAGSK
                     AELETGDILFEEMKRLGLSDVHKDKICVDSWDFHHAVLRYKDREGKEHEFQLGAYQTG
                     FHTEGFQKFSMVYLGRGTAEQYENIDVTGKLVLIDINQREEWWINFPVYQAHLKGAAA
                     LIAVQDNGYGEIHNTALNAQDIAGPKEAAAFSISQADAAVLKADLALAAAEGHRAYGE
                     TTVLFDACSSVIRDQETFNIVGSIPGRETDSMILLSAHYDSYFTGFQDDNAAVAMMFG
                     IARAILKTGYKPNKTLVFCALAAEEWGVVNSKYDWSAGAYEQVFTARPEWQGKVFADL
                     NFELPAHAHGPKDGVRCTYEYASFMEEFVKRINNIPTAYPEGLEVVYPIQTWSDDFSM
                     AIAGIPSMVNEFSAGEFMETHYHSQFDNEQYYQEDVFRFHHELYGRLVLAIDRAAVVP
                     LDFGRLFQAVKASVDPDMCGRTGAGEEELLKKLNRAGKLAEDLYDRVKAVNHRYGELL
                     EADRENGQGEGTENDLRKLERCCREFNRRLLYIFRKEQDYFVRLNWHDEVLFPQEAAG
                     SNLEAIYKALDCLRQGDAQGSLDAVYGIDNNRYAFQFDREVFRYFTEYVLNQPADRLK
                     WGAGRIIRHENLFELVTDLKNAQETVGGHGADLKNEYERLLKVAGNQEQCYRDDIRYM
                     EASVEKLIGQMEECLKVFDREVQFS"
     gene            6582..7217
                     /locus_tag="ACZPCG_05055"
     CDS             6582..7217
                     /locus_tag="ACZPCG_05055"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1284478.1"
                     /translation="MESDKLYRVKKEDLPRLQELLVECFSGDPLYCQLIPDQETRNRL
                     LPELFECDLTEFFETCEIFAESPDMNALLVVSDESEPYNPLTFYLAEAWAGLRTDEYL
                     IKEDPSLKTLWNFVKGRDYLNSRWTDQLHQEERLHVIYLAVRPTMQHHGLAAVLMEEV
                     IAYAQSHHLMVSLETHNPANVPMYEHFGFKIFGIVQKNFDLKQYCMIREFR"
     gene            7396..8493
                     /gene="leuB"
                     /locus_tag="ACZPCG_05060"
     CDS             7396..8493
                     /gene="leuB"
                     /locus_tag="ACZPCG_05060"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HIR1284479.1"
                     /translation="MDYNVTLIPGDGIGPEIVREAKKVMDRVGEVYGHCFQYKEILMG
                     GCSIDAYGVPLTDEAMETARESDAVLLGAVGGDVGNSRWYDVAPDKRPEAGLLAIRKG
                     LGLFANIRPAYLYSQLAKACPLKEEIIGDGFDMVIVRELTGGLYFGARHTEEVDGVMT
                     AVDTLTYNEKEICRIAVKAFDIAMKRRKKVTSVDKANVLDSSRLWRKVVEEVAKDYPE
                     VELSHMLVDNCAMQLVMNPGQFDVILTENMFGDILSDEASMITGSIGMLSSASLNETR
                     FGLYEPSHGSAPDIAGKDLANPIATILSAAMMLRYSFDLDQEADAVEEAVRQVLEDGY
                     RTGDIMAEGCKKVGTREMGDQIAARICRTEA"
     gene            8529..10196
                     /gene="ilvD"
                     /locus_tag="ACZPCG_05065"
     CDS             8529..10196
                     /gene="ilvD"
                     /locus_tag="ACZPCG_05065"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HIR1284480.1"
                     /translation="MKSDAVKTGMQQAPHRSLFNALGMTEEEMKKPMVGIVSSYNEIV
                     PGHMNLDKIVEAVKLGVAMAGGTPVVFPAIAVCDGIAMGHIGMKYSLVTRDLIADSTE
                     CMALAHQFDALVMVPNCDKNVPGLLMAAARINVPTVFVSGGPMLAGHVKGHKTSLSSM
                     FEAVGAYTAGTITEEDVKEYERKTCPTCGSCSGMYTANSMNCLTEVLGMGLKGNGTIP
                     AVYSERIKLAKHAGMQVMEMYRRNIRPRDIMTKEAFMNAMTMDMALGCSTNSMLHLPA
                     IAHEAGVDLNVDIANEISARTPNLCHLAPAGPTYMEDLNEAGGIYAVMNEISKKGLLN
                     LDCMTVTGKTVGENIKNCPNLNPEVIRPVENPYSQTGGIAILKGNLAPDSAVVKRSAV
                     APEMLKHEGPARVFDCEEDAIAAIKGGKIVAGDVVVIRYEGPKGGPGMREMLNPTSAI
                     AGMGLGSTVALITDGRFSGASRGASIGHASPEAAVGGPIALVEEGDRISIDIDNHSLN
                     VLVSDEELEARRAKWQFREPKVTSGYLARYASLVTSADKGAILQVKQ"
     gene            10239..11921
                     /gene="ilvB"
                     /locus_tag="ACZPCG_05070"
     CDS             10239..11921
                     /gene="ilvB"
                     /locus_tag="ACZPCG_05070"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008719482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HIR1284481.1"
                     /translation="MSKLTGAEIVIECLKEQGVDTVFGYPGGAILNIYDALYQHQHEI
                     THILTSHEQGASHAADGYARATGKVGVCLATSGPGATNLVTGIATAYMDSVPVVAITC
                     NVANSLLGKDSFQEIDITGVTMPITKYNFIVKDINKLAKVIRRAFTIAQTGRPGPVLI
                     DITKDVTAAVCEYEPQAPEPIVRQSDSILEEDVEQAISMIRKAQKPFIFVGGGAVISN
                     ASEELRAFAKKIQAPVADSLMGKGAYDGTDELYTGMVGMHGTKTSNFGITEADLLIVV
                     GARFSDRVTGNASKFAKNAKILQIDIDRAEINKNIHVDARIIGDAKVILRKLNARLDP
                     INHDEWIAHIERMKDMYPLRYDKSVLTGPFIIQTINEMTKGDAVIVTEVGQHQMWAAQ
                     YYEYRYPRSLLTSGGLGTMGYGLGAAIGAKMGCKDKTVINIAGDGCFRMNMNEIATAT
                     RYNIPVIQVILNNHVLGMVRQWQTLFYGKRYSQTVLNDAVDFVKIAEAMGAKAYRVTQ
                     KEELEPVLREAISLNIPVVIDCQIGCDDKVFPMVSPGAPIADAFDDTDLKIG"
     gene            12189..13280
                     /gene="serC"
                     /locus_tag="ACZPCG_05075"
     CDS             12189..13280
                     /gene="serC"
                     /locus_tag="ACZPCG_05075"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HIR1284482.1"
                     /translation="MSRVYNFSAGPAVLPEAVLKEAAAEMLDYRGTGMSVMEMSHRSK
                     SYDDIIKAAESDLRDLLQIPDNYRVLFMQGGASQQFAMVPMNLMKNRVADYIITGQWA
                     KKAHKEAGMYGQANAVASSADKTFSYIPDCSDLPISENADYVYICENNTIYGTKFWIL
                     PNTKGKDLVADQSSCFLSEPVDVTKYGLIFAGAQKNVGPAGTVIAIIREDLIREDVLD
                     GTPTMLRYKTHADAGSLYNTPPTYGIYICGKVFKWLKEMGGLEEMKKRNEKKAKLLYD
                     FLDQSRLFKGTVEKKDRSLMNVPFITGNEEMDALFVKESKAAGLENLKGHRTVGGMRA
                     SIYNAMPMEGVEKLVAFMDEFEKKWGQGK"
     gene            13277..14440
                     /locus_tag="ACZPCG_05080"
     CDS             13277..14440
                     /locus_tag="ACZPCG_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HIR1284483.1"
                     /translation="MKKIQCLNPIAACGTELFPADYTFTDQMAEADAVLVRSASMHEL
                     ELPEGLLAVGRAGAGVNNIPLDECAKKGIVVFNTPGANANGVKELVLAGLFLASRDIA
                     GGLAWCKENAADPQIAKTTEKSKKAFAGCEIKGKKLGVIGLGAIGAEVANAATHLGMD
                     VYGYDPYISVNAAWRLSRNVKHITNVDTIFQECDYITVHVPLMESTKGMINKEKLDMM
                     KDGVVVLNFARDTLVNDDDMAEALASGKVARYVSDFPNPKAANMDRVILTPHLGASTK
                     ESEDNCAVMAVKELTDYLENGNIRNSVNFPACDMGVCQAESRIAVLHMNIPNMIGQIT
                     GILAAQGVNITDMTNKSRDKYAYTLLDLEHKPEAATIEKLKAIEGVLRVRKVK"
     gene            14610..15848
                     /locus_tag="ACZPCG_05085"
     CDS             14610..15848
                     /locus_tag="ACZPCG_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HIR1284484.1"
                     /translation="MAAVKPFICIRPRGEMAARTAALPYDVYNRQEACEAVKGNPYSF
                     LNIDRPETQFPAEVDTYDDRVYEKARELLDSQIKEGIYVTDSDPAYYLYELTMDGRSQ
                     TGIVACCAVDDYINGVIKKHENTREDKEQDRIRHVDATNAHTGPIFLAYRRQEELKRL
                     VEQAKKEKALYDFVSEDGIRHRVWKIGDPGMVKAVEEAFEKLPAAYIADGHHRAASAV
                     KAGLKRRKENPDYTGKEPFNYFLSVLFPDDELMILPYNRVVKDLNGLEKEGFLKKAEE
                     KFEIHLLGKEAFAPDQKGTFGMYLEGEWYGLKIRPEYESEDPVAGLDVSILQDQLLGP
                     VLGIGDPRTDCRIDFIGGIRGLKELERRADSDMKLAFSMYPTSITELLAVADAGQLMP
                     PKSTWFEPKLRSGLFIHRLS"
     gene            complement(15829..17046)
                     /locus_tag="ACZPCG_05090"
     CDS             complement(15829..17046)
                     /locus_tag="ACZPCG_05090"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR1284485.1"
                     /translation="MKLFPHFISHISSFFHKHSQAVIWAELAALVFLFSGLFPAFHQK
                     ALNVLSGRPLASSSDASKAPAKSSIKWVDFNVTAEALNQAFRYDVDTVQSPIHLNWVD
                     LLALLGSRYGGDFSSYRPKDMEAMAEAVLKGETTMDTLKEKSKNFDYYREAYGAVLDG
                     MVGVFKAEIPVSEAPPEYVSENQDQDSGTIWVTKYGLKAFSPIAKGFPYNDYDDFGAA
                     RSYGFKRQHLGHDMMGQVGTPIISVESGYVEAIGWNPYGGWRIGIRSLDQKRYYYYAH
                     LRKNYPYQSNLSQGSYVQAGDVIGYLGRTGYSTTENTNNIDTPHLHFGLELIFDEARR
                     QEGKEVWIDCYGLIQFLERSRCETVKVEGTKEWIRVYGMKEADPQRGGNPPEEGSSPE
                     EGSSLAPSMTGGG"
     gene            17227..>17889
                     /locus_tag="ACZPCG_05095"
     CDS             17227..>17889
                     /locus_tag="ACZPCG_05095"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIR1284486.1"
                     /translation="MENFEFYAPTRMIFGKDTHLKVGKLIREYGFKKVLIHFGGGSVK
                     RTGLLDTVCRSLEEEGIGYVLLGGVQANPVLSMARKGIELCRREQVDFILAVGGGSVI
                     DSSKCIADGVGNPDVDVWKFHMKEAVPKKALPVGVILTLAAAGSEMSASCVITNEENG
                     LKRGFNSVTHRPLFSICNPELTYTVNAFQTGCGTVDIMMHTLERYFSLGHDTSLTDRM
                     AEG"
BASE COUNT         4988 a         3671 c         5072 g         4158 t
ORIGIN      
        1 ggtattgcgg gctttattga cgcggagcat gcattggatc cggtttatgc ccggaatatt
       61 ggcgtagata ttgataatct ttatatttct cagccggata atggagagca ggctctggag
      121 atcacagaga ccatggttcg ttccggtgct gtggatatcg tgatcgtgga ctctgttgca
      181 gctcttgttc caaaggcaga gattgacgga gagatggggg attcccatgt gggacttcag
      241 gcccgtctga tgtctcaggc ccttcgaaag ctcactgcca ttatcagcaa gtctaactgt
      301 gtggttattt ttatcaatca gcttcgtgaa aaggtcggcg ttatgtttgg aaatcctgag
      361 gttactactg gtggacgcgc tctgaagttt tatgcgtctg tccgtatgga cgtccgcagg
      421 atagagacct taaagcaggg aggggaggtt gtaggaaacc gggtccgcgt aaaggttgtg
      481 aagaataaga ttgctcctcc ttttaaagag gcagagtttg atatcatgtt tggaaagggg
      541 atttccaaaa caggcgatat cctggatttg gccgtaaagg aaaatatcgt ggaaaagagc
      601 ggtgcatggt ttgcatatgg cggttccaaa atcggtcagg gacgtgaaaa tgccaagcag
      661 tatctggcag agcatcctgc gatctgtgca gagatcgagg cacaggtgag agtgcgctat
      721 cagcttccgg gagcagagga ggctgaggca gaggccagaa aggcagcgca ggcagagaat
      781 gtccggcctg catcctctca aagtgaagca aaagaagatc ctgcggcaga accggcagcg
      841 gatgaagaag catgagggtc acagcggtga cccctgtgga taagagaaag tgcaaagtct
      901 tcctggaaga ggaggacttt gcttttcctt tatacagagg ggaggcagag cggtttcata
      961 ttcaggaagg ctctgaactg ccggaaactg tatatctccg gatcgtagaa gagattttgg
     1021 ttcccagggc aaaagagcgg gctctttacc ttctgcaggc tcagggaagg actggagcac
     1081 agatggaaac gaagctttca ggtgacggat atccgaaaga ggttataaac agggtgatgg
     1141 aatttctgaa ggaataccgt ctgattgatg acagagtcta tacagagaat tttatccgta
     1201 tgaatcagga gaaaaaaagc cgtcgccaga tagcgtttga gctgcagcaa aagggagtgg
     1261 accgccagga tatttccagg attctggaag gaagcgctca ggatgacctt gcggctgcca
     1321 gaaatctttt gaaaaaacgc cttcgaactg cagatctttc tgatctcaaa gagaaaaatc
     1381 gcctgggggc gtatttggga agacggggct tttcctatga cgtgatccgc agggtgatgc
     1441 aagaggttca aagtgggcaa gaggagtaga agaagccgta atttcaggaa aatgttttac
     1501 aggatgcaca aaagccgggg aaatcaagac ttgacactct ttacaaaaaa gtataaaatt
     1561 aatatgttgt attttgtaca atgaaaacta aataaggagg tgctcctgtg ttacagatag
     1621 tggtcacttt gatgatcggt gcggcaatcg cggctgtgat cacctggttt attgcccatt
     1681 ccgccggtcg ttcctatgag cgcaagcagt atgaattaaa ggtaggaagc gcggaagaaa
     1741 agtccagaga gatcattgac gaagccttaa agaccgcaga gacaaagaag cgagaagctc
     1801 tcctggaagc caaggaagaa tctttaaaga caaagaatga actggaaaag gaaaccaagg
     1861 aaagaagagc ggaacttcag cgttatgaaa agcgtgtgct gagcaaggaa gaaaacgttg
     1921 aaaagaaggc agatgccatg gaaaagaagg aggcagatct gatcagaagg gagaatatac
     1981 tcggcaaaag aacggctgag gtagaagccc agtatgagca gggaatacag gaattggaac
     2041 ggatatccgg tctgacctcc gaacaggcaa aagattatct tttaaaatct gttgaagaag
     2101 acgtaaaaca tgacacagct aaactgatca aggaacttga aaacaaggca aaagaagaag
     2161 ctgataagaa agccaggaat ctggttgtca cagccatcca gcgctgtgcg gcagaccatg
     2221 tggctgaaac tacagtatct gtggtacagc ttcccaatga tgagatgaag ggccgcatta
     2281 tcggccgtga gggacggaat atccgtactc tggaaacact gacaggtgtg gaattgatca
     2341 tcgacgatac tcccgaagcg gttgttttat ccgggtttga ccccatccgc agggaagtag
     2401 caagaatcgc attggagcgt ttgattgtgg acggacggat ccatccggcc agaatcgagg
     2461 aaatggtaga aaaagcacgg agagaagtgg aaaacaatat gcgggaagag ggcgaagctg
     2521 cctgtctcga agttggtatc cacggaattc atccggaact ggtaaagctg cttggaagga
     2581 tgaaattcag gacaagctat ggacagaatg ccttaaagca ttccatcgaa gtagcgcagt
     2641 tatcggggct tttagcatca gaacttgggg tggatgtgcg tttggcaaaa cgtgctggat
     2701 tgttacatga cattggtaaa tccgttgacc atgatatgga aggcacccat gtacagctgg
     2761 gagcagacct gtgcagaaag tacaaggagt ctgcagttgt attgaatgcg gtagaatccc
     2821 atcatggcga tgttgaacct accagcctta tctcctgtat cgttcaggcg gcagatacga
     2881 tttctgctgc aagacccggt gcaagacgtg agacgctgga gacatataca aacagattga
     2941 aacagttaga agatatcaca aactccttta agggagtaga caaatctttt gctattcagg
     3001 caggccgtga ggttcgtatt atggttgttc ctgagcagat cagcgatgac gatatggtat
     3061 tgctggcaag ggaagtatcc aagcgcattg aaagcgaact ggaatatccg ggacagatca
     3121 aggtaaatgt gatccgtgaa tccagggtaa ccgattacgc gaaataggaa gaaagcttcg
     3181 tcaggagaac agctggcgga gctttttttg tgtgggaacg cgataaaaag gaatgacagg
     3241 caaatggaat tgtaaggaaa aattcattga cactccatgt gaaaatttat attctagtga
     3301 taggatcgca tctaatatat acaagaacag ggaggtttgc atatgataca tttaaaaaag
     3361 aggctggcct ttggactggc tgcaggactt ttggcagcct gtataacagt tcctgtttat
     3421 ggagcaagcc gtaaaaagat cacatctatt tcactgacga tcaaggcgga tattgaggca
     3481 gatacagatt atggcgatga gacgattgaa attgaaagca gcagcagccg ttacaatgta
     3541 gacggttatg aggtaatgaa cgaagggttt ggatggacgg aggatacggt tccggaaatc
     3601 cggattatgc ttactgccaa tgacgattat tattttacat ctcttggaac ggataagatt
     3661 accctgaagg gtggagctga gtttaaaaag gctaccagac aggattccag tgaaacactg
     3721 cttttggatg ttacccttcc ttccatgcag aattccttaa aggatatgga aggcgtaacg
     3781 ctttctcagg aagggattgc gtcatggcct gtcatcggca cagcgggaag ttacgaagtg
     3841 agagtataca ggggagataa aattgtagga gcttccctga caacggagac aaacagcatc
     3901 aactgccggg agcgcatgtt aaagccaaat gaatcttaca tggtaaaggt ccgtgctgtg
     3961 aataagtttg atccgaccat taagggcgag tgggtttcct ccaacactat ctatgtaaat
     4021 ggtgggcagg ccgctgcctt ccgggagaat ccggatggtg caggagtaga gggaggatca
     4081 ggagcagccg gtgagtggaa acagacagaa gacggaagat ggtggtatga tcagggagac
     4141 ggaacgtatc ctgctggagg ctggctggaa attggacata agtggtattt ctttgacgca
     4201 gagggctata tgcagacagg atggattccc tggaatgaaa aggagtatta ttgctctgaa
     4261 aatggagata tgctcacaaa ctgcattact ccggatcatt atctggtagg agaggacgga
     4321 gccaaaattg ctcagtagaa gagaggcggc tgtcaacaaa aatggaacat caggtgcagc
     4381 aggaggaagc tccggccgcg ccttttttcc ttaaagcgtt tacaaaacag gcaagaagca
     4441 agtgaggtaa cagaaaatgg ataaatggaa gaattgggaa cagaagttga tcgaagcagt
     4501 cgatacagaa tattcctacc gtctggcaaa gaggatggag gaaccaaaaa ccaatcctgt
     4561 actgggatac cggactgccg gatccaaagc agagcttgag accggggata ttctgtttga
     4621 ggaaatgaag cgtctggggc tttcggatgt gcataaggat aagatctgcg tggacagctg
     4681 ggatttccac catgcagtgc tgcgctataa agaccgtgaa ggaaaggagc atgaatttca
     4741 gctgggagca taccagacag ggtttcatac agaaggcttt cagaagtttt ccatggtata
     4801 tctgggccga ggaacggcag aacagtatga aaatatagat gtcacaggca aactggtcct
     4861 cattgatatc aatcagagag aagagtggtg gattaatttt ccggtctatc aggctcattt
     4921 aaagggagcg gcggctctga tcgcagttca ggataacgga tatggggaaa tacataatac
     4981 ggccttaaat gcgcaggata tcgcaggacc gaaggaggct gctgcttttt ctatttctca
     5041 ggcggacgca gctgtgctca aagcagatct ggcgttggcg gcagcggaag gacaccgggc
     5101 atatggggag acaacggtgc tgtttgacgc ctgttcttcg gtcatcagag atcaggaaac
     5161 ctttaatatt gtaggaagca tcccagggcg tgagacggat tccatgatcc tgctatcggc
     5221 ccattacgat tcctatttta cgggctttca ggatgataat gcggcagttg ccatgatgtt
     5281 tggcattgca agggctattt taaagacagg atataagccg aataagacgt tggtgttctg
     5341 cgcgctggct gcggaggagt ggggcgtagt gaattccaaa tatgactggt ctgccggagc
     5401 ctatgagcag gtcttcacag cccggccgga atggcaggga aaggtgtttg cagacttaaa
     5461 tttcgaactt ccggctcatg ctcacggccc gaaagacgga gtgaggtgta catatgaata
     5521 tgcttcgttt atggaagaat ttgtaaaacg cattaacaac ataccgactg cttatccgga
     5581 agggctggaa gtggtatatc ccatccagac ctggtcagat gatttttcca tggctattgc
     5641 gggaatcccg tctatggtaa atgaatttag tgcgggagag tttatggaga cccattacca
     5701 ttcccagttt gacaatgaac agtattatca ggaggacgta ttccgtttcc accatgagct
     5761 ctatggcaga ctggtacttg ccattgaccg ggcggcagtg gttccgctgg actttggacg
     5821 attgtttcag gcagtaaaag ccagtgtaga cccggatatg tgcggcagaa ccggagccgg
     5881 tgaggaagaa cttttaaaaa agctgaaccg ggcagggaaa ctggcagaag atttgtacga
     5941 cagagtaaag gctgtgaacc acagatatgg tgagcttttg gaagctgata gggagaatgg
     6001 acagggggaa ggaacagaga acgatctgcg gaaattagag cgctgctgcc gggagtttaa
     6061 ccgccgcctg ctttatatat tcagaaagga acaggattat tttgtgcgtt tgaactggca
     6121 tgacgaagtg ctgtttcctc aggaagcagc aggaagcaat ctggaagcta tttacaaggc
     6181 actggattgt ctgcgccagg gagatgctca gggcagtctg gatgctgtat atgggattga
     6241 caataatcgt tatgccttcc agtttgaccg tgaggtgttc aggtatttta cggaatatgt
     6301 gcttaatcag ccggcagacc ggctgaaatg gggagcagga cgtatcatac gccatgaaaa
     6361 tctgtttgaa cttgtgactg atctgaagaa cgctcaggaa acagttggag gccatggggc
     6421 agatctgaag aatgaatatg agagacttct taaggtagca ggaaaccagg aacagtgcta
     6481 ccgggatgat atccggtata tggaagcttc tgtggaaaag ctgatcggac agatggaaga
     6541 gtgcctgaag gtttttgaca gggaggtgca gttttcgtaa tatggaaagt gataaattat
     6601 acagggtaaa aaaagaggat ctgcccaggc ttcaggagct tttggtggaa tgtttttctg
     6661 gagatcctct gtactgccag ctgatcccgg atcaggaaac aagaaatagg ctgcttccgg
     6721 agctgtttga atgcgatctc acggaatttt ttgaaacctg tgaaattttt gcggaaagtc
     6781 ctgatatgaa tgctctgttg gtcgtatcag atgaatcgga accctataat cctctgacct
     6841 tttatctggc agaggcctgg gccggcctga gaacggacga gtatctgata aaagaagatc
     6901 ccagtttaaa gacactgtgg aattttgtaa aagggcggga ttatttaaat tcccgctgga
     6961 ccgatcagct tcatcaggag gaacgccttc atgtgatcta tctggcagta cgccccacta
     7021 tgcagcacca cgggctggcg gctgttctca tggaagaggt gattgcatac gctcagtctc
     7081 atcacctgat ggtgtctctt gaaacacata atccggccaa tgttcccatg tacgagcatt
     7141 tcggctttaa gatctttgga attgtacaga aaaactttga ccttaagcag tactgcatga
     7201 tccgggaatt caggtagaag ataaatgcgg tataactttt gattatacag tcaattccaa
     7261 aattgtatat ttgcaagtac aaaaattcag acagaacggt tgaaatacac ccctttatga
     7321 gatatgataa aaagaaattg tcagcgatag gaaagatata acctgagtga atagtaataa
     7381 agggggaatg ctggaatgga ttataatgta actctgattc ccggagatgg gattggtcct
     7441 gagattgtaa gagaagccaa aaaggtgatg gatcgtgtgg gagaagtata tggtcattgc
     7501 tttcaatata aagagatcct gatgggcggc tgttccattg acgcatatgg agtgcccctt
     7561 acagatgagg cgatggagac tgccagagag agtgacgcag tccttttagg agcagtggga
     7621 ggagatgtgg gaaattcccg ctggtatgat gtggcaccgg ataagcgtcc tgaggcaggg
     7681 ctgttagcca tccgcaaggg actgggactc tttgccaaca tccgtccggc ctatctctac
     7741 agccagcttg caaaggcctg tcccttaaag gaagaaatca tcggagacgg ctttgacatg
     7801 gtgatcgtga gggaactcac gggaggcctg tatttcggag cacgtcatac agaagaggtg
     7861 gacggagtta tgacagccgt ggataccctt acctacaatg agaaggaaat ctgccgtatt
     7921 gcggtaaaag cctttgatat cgccatgaag cggcgcaaaa aggttaccag cgtggataag
     7981 gccaacgtgc tggattcctc caggctctgg agaaaggtag tggaggaagt ggcaaaggac
     8041 tacccggagg tagagctgtc ccatatgctg gtggacaact gcgccatgca gctggtgatg
     8101 aatccggggc agtttgacgt gatccttacg gaaaatatgt ttggcgatat tctttccgac
     8161 gaagccagca tgatcacagg ctctatcggt atgctgtcct ccgccagctt aaacgaaacc
     8221 cgcttcggcc tctatgagcc aagccacggc tccgcgccgg atatcgcggg aaaggatctt
     8281 gcaaacccca ttgcgacgat actttctgcc gcaatgatgc tgcggtattc ctttgacctg
     8341 gatcaggagg ctgatgcagt ggaggaggca gtccgccagg tactggagga cggatatcgc
     8401 acaggcgata ttatggctga aggctgcaaa aaggtgggta ccagagaaat gggcgatcag
     8461 attgcagcgc gcatctgccg gacagaggct taaatacaga aataacccca taaaaggaga
     8521 tcggacatat gaaaagtgat gcagtaaaga ccggtatgca gcaggcgcct caccgctccc
     8581 tgtttaatgc ccttggaatg acggaagaag agatgaaaaa gcccatggtg ggtattgtaa
     8641 gctcctataa tgagatcgtg ccgggccata tgaatctgga taaaattgtg gaggctgtaa
     8701 aactgggcgt agccatggca ggaggtactc cggtggtgtt tccggctatt gcagtctgcg
     8761 acggcattgc catgggccat atcggcatga aatattctct ggtaacaaga gacctgattg
     8821 cagattctac agagtgcatg gcgctggccc accagtttga cgcccttgtt atggttccca
     8881 actgcgataa aaatgtgccg gggcttctga tggctgcagc caggatcaat gtgcctaccg
     8941 tatttgtcag cggcggcccc atgctggcag gccacgtaaa gggacacaag accagtcttt
     9001 ccagtatgtt tgaggcagtg ggtgcctata cagccggaac cattaccgag gaagacgtaa
     9061 aagaatacga gagaaaaacc tgccctacct gcggctcctg ctccggcatg tatacagcta
     9121 acagcatgaa ctgcttaaca gaggttctgg gaatggggct taagggcaac ggaaccatcc
     9181 cggctgttta ctccgaacgt attaagctgg caaagcacgc cggcatgcag gttatggaga
     9241 tgtaccgcag aaatatccga ccaagggata ttatgacaaa agaagccttt atgaatgcta
     9301 tgactatgga catggccctg ggctgctcca ccaacagtat gctccatctt ccggccattg
     9361 ctcatgaggc gggcgtggat ttaaacgtgg atattgccaa tgagatcagc gccagaaccc
     9421 cgaatctctg ccacctggct cctgcaggcc ctacctatat ggaggactta aacgaggccg
     9481 gaggcatcta tgcggtgatg aacgagatca gcaagaaggg acttttaaat ctggactgca
     9541 tgaccgtaac gggaaagacc gtgggagaaa atataaagaa ttgtccaaac ttaaatccag
     9601 aggtgatccg cccggtagaa aatccctaca gccagaccgg cggcatcgcc atcctaaagg
     9661 gcaatctggc cccggattcc gccgtggtaa agcgttctgc cgttgcgcct gagatgttaa
     9721 agcatgaagg tccggccaga gtctttgact gtgaggagga tgctattgca gccatcaagg
     9781 gaggaaagat cgttgcggga gatgtagttg tgatccgcta tgaaggcccc aagggcggcc
     9841 ccggtatgag agagatgctc aatcctacct ccgctattgc aggaatggga ctgggctcta
     9901 cggtagctct gatcacggac ggacggttct ccggcgcttc cagaggcgct tccatcggcc
     9961 atgcatcccc ggaggcagca gtgggcggcc ctattgcact ggtggaagaa ggagaccgga
    10021 tttccattga tattgacaat cattccctga atgtccttgt atccgatgag gagctggaag
    10081 caagaagggc aaaatggcag ttcagagaac cgaaggtgac ctcggggtat ctggcaagat
    10141 atgcctccct tgtgacctcc gcggacaagg gcgctattct tcaggtaaag cagtaagaag
    10201 ataaaatcaa aatccctttg agaaggagta acaggactat gagtaaattg acgggggcag
    10261 aaatcgttat tgaatgttta aaggaacagg gagtggacac ggtgtttggc tatccgggcg
    10321 gagccatcct gaatatatac gatgcgctgt atcagcatca gcatgagatt acccatattc
    10381 ttacctctca tgagcaggga gcttcccatg cggcagacgg atatgccaga gccacaggaa
    10441 aagtaggagt gtgcctggct acttccggcc ccggtgcgac caatctggta acaggaatcg
    10501 ccaccgccta tatggactcc gttcccgtag ttgccattac ctgcaacgta gctaacagcc
    10561 tgctgggaaa ggacagtttc caggagattg atattacagg cgttaccatg cccattacca
    10621 agtacaattt cattgtaaaa gatataaata agctggcaaa ggtgattcgc cgtgctttca
    10681 cgatagccca gaccggccgt cccggcccgg tgctgatcga tatcacaaaa gatgtgactg
    10741 cggctgtatg tgaatatgag cctcaggctc cggagcctat tgtgcgtcag agcgattcca
    10801 ttttagagga ggatgtggag caggctattt ccatgatccg caaagctcag aagcccttta
    10861 tttttgtagg aggcggagct gtgatctcca atgcatcgga ggaattgcgg gcatttgcca
    10921 aaaaaataca ggctccagtg gcggacagcc tgatgggaaa gggtgcttat gacggcaccg
    10981 atgagcttta tacaggcatg gtaggcatgc acggcacaaa aacctcaaat ttcggtatca
    11041 cagaggcaga tctgctgatc gtagtgggag cacgcttcag cgaccgcgtg acgggaaatg
    11101 cctccaaatt tgcaaaaaat gccaaaattc tccagattga tattgaccgt gcggaaatca
    11161 ataaaaatat ccatgtggat gcccggatca tcggagacgc caaggtcatc ttgagaaagc
    11221 tcaatgccag gctggatccc atcaaccatg acgaatggat cgcccatatt gagcggatga
    11281 aggatatgta tcctctgcgt tacgacaagt ctgtgcttac agggccgttt atcatacaga
    11341 ccatcaatga gatgacaaag ggagacgctg tgatcgtcac tgaggtggga cagcatcaga
    11401 tgtgggcggc ccagtattat gaataccgct atcccagatc ccttttaacc tccggcggtc
    11461 tgggaaccat gggctatggc ctgggagcag ccattggagc caagatgggc tgtaaggaca
    11521 agacggtgat caatattgcc ggagacggct gcttccgcat gaatatgaac gagatcgcta
    11581 ctgccacccg ttacaatatc cctgtgattc aggtgatatt aaataatcat gtgctgggaa
    11641 tggtgcgtca gtggcagacg ctcttttacg gaaagcgcta ttcccagacg gtgctcaatg
    11701 atgcagtgga ctttgtaaag attgcagagg ccatgggcgc aaaggcgtac cgtgtgaccc
    11761 agaaggagga gctggagccg gttttaagag aggccatcag cctgaatatt cccgttgtga
    11821 tcgactgtca gatcggctgt gatgacaagg tatttcccat ggtatctccg ggagcaccca
    11881 ttgcagatgc ctttgatgat acagatttga aaattggata gtaaatatac agccggcagg
    11941 aaggtattaa gtcgccttcc tgatctggct gtattttttt ggaaatacta agagaaaaga
    12001 aaaaatattc tgaaatttat gcttgaaaat ttcgtaaaaa aggagtatag tgttttcaac
    12061 gcattatcac tacatcatga taaagcgaat ataggcggac agtctgcata gtgaaaaact
    12121 gtctgcatat atataaatac aggtgcaggg aaaaaaatga aaggcaccca gaataaggag
    12181 gagacaggat gagcagagtt tataattttt ccgcaggtcc ggctgtgctg ccggaggctg
    12241 ttctgaagga agctgcagca gagatgctgg attacagagg cactggaatg tcagtgatgg
    12301 agatgagcca ccgttcaaaa tcatacgatg acattatcaa ggctgcggaa tcagatctgc
    12361 gggacctttt acagattcct gataattacc gggtgctttt catgcagggc ggcgcatccc
    12421 agcagttcgc catggttccc atgaacctga tgaagaaccg ggtagcagat tacattatca
    12481 ccggacagtg ggcgaaaaag gcccataagg aagccgggat gtacggacag gcaaatgctg
    12541 ttgcatccag tgcagacaag acattttcct atattcctga ctgttccgat cttcccattt
    12601 cagaaaatgc ggactatgtg tatatctgtg agaacaacac catttacgga accaagttct
    12661 ggatccttcc caatacaaag ggcaaggatc tggtggcaga ccagtcttcc tgcttcttat
    12721 ctgagccggt ggatgtgacg aaatacggcc ttatttttgc aggtgcccag aaaaatgtag
    12781 gacctgcagg aacggtcatt gccattatca gagaggatct gatccgagag gatgtactgg
    12841 atggaacacc caccatgctg cgctacaaga cccatgcgga tgcaggctcc ctgtacaaca
    12901 ccccccctac ctatggcatt tacatctgcg gcaaggtatt taagtggcta aaggaaatgg
    12961 gcggtctgga ggaaatgaaa aagcgcaacg agaagaaggc aaagcttctc tatgatttcc
    13021 tggatcagag ccgtctcttt aagggaacag tggaaaagaa ggaccgttcc cttatgaatg
    13081 ttccttttat cacgggaaat gaggaaatgg atgcgctttt tgtaaaggag tctaaggcag
    13141 cgggtcttga aaacttaaag ggacaccgta ccgtaggagg catgagagcc agcatttaca
    13201 atgcaatgcc catggagggc gtggagaagc tggtagcctt tatggatgag tttgagaaga
    13261 aatgggggca ggggaaatga aaaagattca gtgcttaaat ccaattgctg cctgcggaac
    13321 tgagctgttc ccggcagatt atacctttac cgatcagatg gcagaggcag atgcggttct
    13381 ggtgcgcagc gcctccatgc atgagctgga gcttccggaa ggacttctgg ccgtaggccg
    13441 tgccggagcc ggtgtgaata atatccctct ggatgaatgt gccaaaaagg gaatcgtggt
    13501 atttaatact ccgggagcca atgcaaacgg cgtaaaggag ctggttttgg cagggctctt
    13561 cctggcttcc agagatattg caggaggact tgcatggtgt aaggaaaatg ccgcagatcc
    13621 ccagattgcg aagacaacgg aaaagagcaa aaaggccttt gcaggctgtg aaataaaggg
    13681 aaagaaattg ggtgttatcg gtcttggagc catcggcgca gaagtggcaa acgcagctac
    13741 ccatctggga atggatgtat atggctatga tccctatatc tctgttaatg ccgcctggag
    13801 gctttccaga aatgtaaagc atattaccaa tgtggacacc attttccagg agtgtgatta
    13861 cataaccgtt catgtgccgc ttatggagtc tacaaagggc atgattaata aagaaaagct
    13921 ggacatgatg aaggatggcg tggtagtatt aaactttgcc agagacacac tggtaaatga
    13981 tgacgatatg gcagaggcgc tggcatccgg taaggtggcc cgctatgtaa gcgacttccc
    14041 caatcccaaa gcagccaata tggatcgtgt gatcctgacg cctcatctgg gagcatccac
    14101 aaaggagtct gaggataact gtgcagtgat ggctgttaaa gagctgacgg attatctgga
    14161 aaatggaaac atccgcaatt ccgtgaattt cccggcctgc gatatgggtg tatgccaggc
    14221 agagagccgg atcgcggtgc ttcatatgaa tatcccaaat atgatcggac agattacggg
    14281 aattctggca gcccagggcg taaatattac ggacatgaca aataagagcc gtgataagta
    14341 tgcttatacg cttctggatc tggagcataa gccggaagcg gctaccattg agaaattaaa
    14401 agccatcgag ggtgtgctca gggtgcgcaa ggtaaaataa gtgataatga agcctgtgat
    14461 gttaaggcat cggcagtgtg aattgtaaga gccttcatgg ccggccggct gtggaggctt
    14521 tttggtctct gcgatcagcc ggatggctgt gagggctttt tgagtctctg gcctctgcgg
    14581 acagggagaa tagaaagagg agaaaaatta tggcagcagt aaaaccattt atctgtatca
    14641 gacccagggg ggagatggcg gcccgtacag cggccctccc ctatgatgta tataatcggc
    14701 aggaggcctg tgaggccgtc aaggggaacc catattcatt tttaaatatt gaccgtccgg
    14761 agacacagtt tccggcagag gtagacacat atgatgaccg ggtatatgaa aaggccaggg
    14821 agcttcttga cagccagatc aaagagggca tatatgtaac agattccgat cctgcctact
    14881 atctgtatga gcttaccatg gatggacgca gccagaccgg gatcgtggcc tgctgcgcag
    14941 tggatgacta tataaacggg gtcatcaaaa agcatgagaa taccagagaa gacaaggagc
    15001 aggaccgcat ccgccatgtg gatgccacca acgcccatac aggccctatt tttctggctt
    15061 accgcaggca ggaggaatta aaacgcctgg tggagcaggc aaaaaaggag aaggctctgt
    15121 atgattttgt atcagaggac ggtatccgcc acagggtatg gaaaataggt gatcccggga
    15181 tggtaaaggc agtagaggag gcctttgaga agcttcctgc ggcctatatt gcagacggcc
    15241 accacagagc ggcttcagcc gtcaaggcgg gacttaaaag acggaaggaa aacccggact
    15301 acacgggaaa agagcccttt aactattttc tgtctgttct gttccccgat gatgagctga
    15361 tgatcctgcc ctataaccgg gtggtaaagg acttgaacgg tctggaaaaa gagggatttt
    15421 taaagaaagc agaagaaaaa tttgagattc atcttttggg aaaggaggcc tttgccccgg
    15481 atcagaaggg aacctttgga atgtatctgg agggagaatg gtacggtctg aagataaggc
    15541 cggaatatga gagtgaagat ccggtcgcag ggctggatgt atccattctt caggatcagc
    15601 ttttaggacc tgttcttgga atcggtgatc cgagaacaga ctgcagaatt gattttatcg
    15661 gaggaatccg gggattaaag gagttggaac gcagagcgga cagcgatatg aagcttgcat
    15721 tttccatgta tcctacttcc attacagagc ttttggcggt ggcggatgca ggacagctga
    15781 tgccgcctaa atccacctgg tttgagccaa aactcagaag cgggctgttt atccaccgcc
    15841 tgtcatagaa ggagccagag aactgccttc ttccggggaa ctgccttctt cggggggatt
    15901 gccgcccctt tgtggatccg cttccttcat gccatagacg cggatccatt ccttggtgcc
    15961 ctctaccttg actgtttcac agcggctgcg ttccagaaac tggatcagcc catagcagtc
    16021 gatccacacc tcctttccct cctggcggcg ggcttcgtca aagatcagct ccagtccgaa
    16081 atggaggtgt ggtgtgtcga tgttgttggt attttccgta gtgctgtagc cggtgcggcc
    16141 cagatagccg atcacgtctc cggcctggac ataggaaccc tgagagagat tggactggta
    16201 ggggtagttt ttgcgcagat gggcgtagta atagtaacgt ttctgatcca ggctgcggat
    16261 cccaatcctc cagccgccat aggggttcca gccaatggct tccacatagc cggactctac
    16321 ggagataatg ggtgttccta cctgccccat catgtcatgg cccaggtgct ggcgcttaaa
    16381 gccatagctt cttgctgccc cgaaatcatc ataatcattg tagggaaagc ccttggcgat
    16441 gggagaaaag gcttttaggc cgtattttgt gacccagatg gtgccagagt cctgatcctg
    16501 attttcagag acatattccg gcggggcttc gcttacaggg atttccgctt taaatactcc
    16561 taccattccg tccaggacgg ctccgtatgc ttctctgtag tagtcaaagt tcttcgattt
    16621 ttctttcagt gtgtccattg tcgtttcacc ctttaatacc gcttctgcca tggcttccat
    16681 atcctttggc cggtaggagg aaaaatctcc gccgtaccgg ctgccaagga gagcaagaag
    16741 atcgacccag tttagatgaa tcgggctttg aacggtatct acatcatagc ggaaggcctg
    16801 atttaaggct tcggctgtta cattaaaatc cacccacttt atactgcttt ttgccggggc
    16861 ttttgaggca tcggaggaag atgccaaagg gcggccggaa agaacgttta aggctttttg
    16921 gtgaaaggcc ggaaaaaggc cggagaacag aaatacaagt gctgcaagct ctgcccaaat
    16981 gaccgcctgg gagtgcttgt ggaaaaaact ggaaatatgg gaaataaaat gagggaaaag
    17041 cttcatcaaa agtacctctt tagaataagg tcctgagaac agtctatggg gtgggatggg
    17101 aaaatatgat ggtggggaag agatggccaa attaaattga gaaaatgatg cgtatgtggt
    17161 aaaataagga cagaatgcga gccatgtggg gttgcatgag gcggcatagg acaaggagga
    17221 gtaaacgtgg aaaattttga attttatgca ccgacgcgga tgatttttgg aaaggatacc
    17281 catcttaagg tgggaaagct gatccgggag tatggcttta agaaggttct cattcatttt
    17341 ggcggaggaa gcgtgaagcg gacgggtctt cttgatacgg tttgcaggtc attggaggaa
    17401 gaagggatcg gatatgtgct gctaggaggc gttcaggcca atccggttct ttccatggca
    17461 agaaagggta ttgagctgtg ccggagggaa caggtagatt ttatcctggc agtaggcgga
    17521 ggaagcgtga tcgattcctc caagtgcata gcagacggcg tagggaatcc tgatgtggat
    17581 gtatggaaat tccatatgaa ggaagctgtt ccgaagaagg ccctccctgt tggtgttatt
    17641 cttacattgg ctgccgcagg cagtgagatg agcgcctcct gcgtgatcac taacgaggaa
    17701 aacggcctaa agagaggttt taacagtgta actcacaggc ctttattttc catctgtaac
    17761 ccggagctga cctataccgt aaatgccttc cagacaggct gcggcacagt ggatattatg
    17821 atgcatactc tggagcggta tttcagtctg ggacatgata caagccttac agaccgtatg
    17881 gcggaggga
//
LOCUS       DBMDUH010000039        17762 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_1868_length_17762_cov_7.854821, whole genome shotgun sequence.
ACCESSION   DBMDUH010000039 DBMDUH010000000
VERSION     DBMDUH010000039.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 17762)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 17762)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17762
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1868_length_17762_cov_7.854821"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            268..684
                     /locus_tag="ACZPCG_05100"
     CDS             268..684
                     /locus_tag="ACZPCG_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HIR1284487.1"
                     /translation="MEAYMDAKASTCSCGCGCRSRVVPGVSDRPAAQPEYSGPSPQMW
                     PGWDMNQSLPYGEAGYPQTGYPQIGTNMNQGSTVWPIVNCPDTTAGYLEQHHPVAMAY
                     VPWQQWQTAYAPERGLMQGTIFPDLDLPFNYGRCGR"
     gene            681..953
                     /locus_tag="ACZPCG_05105"
     CDS             681..953
                     /locus_tag="ACZPCG_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HIR1284488.1"
                     /translation="MTEHLQNPSRGAMLQAVNQTSFAVVEANLYLDTHPCDMEALSYL
                     NQMMEAYKNAKAAYEARFGPLNAGNVTENAYWTWVSDPWPWEGGNL"
     gene            953..1570
                     /locus_tag="ACZPCG_05110"
     CDS             953..1570
                     /locus_tag="ACZPCG_05110"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HIR1284489.1"
                     /translation="MWIYEKRLQFPVNIKEPNAKLAQFIMSQYGGPDGEMGASMRYLS
                     QRYAMPYKEGKAVLTDIGTEELAHLEMIAAIVHQLTRNLTAEQLVEQGFGPYYIDHAT
                     GIWPQAAGGIPFNACEFQSKGDVITDLHEDMAAEQKARSTYDNILRVVTDYDVCEPIK
                     FLREREIVHYQRFGETLRITQEKLDSRNFYAFNPAFDPDQAPQPR"
     gene            complement(1578..2165)
                     /locus_tag="ACZPCG_05115"
     CDS             complement(1578..2165)
                     /locus_tag="ACZPCG_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1284490.1"
                     /translation="MKLCIEHLSKKYGELQVLRDLSLTLEQDACYCLMSPSGSGKTTF
                     LRILMGLETADSGRIFTQGGPGWPLRISAVFQEDRLCPSFSPMENVQMALKEKWKEEK
                     LAQAMTCLLPKECLTRPVSTLSGGMKRRTAILRAVPAPSHMLVMDEPFTGLDEALKLE
                     VIRYILENRRNRFLLITTHQEEDVKLLGGVRIQLD"
     gene            complement(2178..2915)
                     /locus_tag="ACZPCG_05120"
     CDS             complement(2178..2915)
                     /locus_tag="ACZPCG_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1284491.1"
                     /translation="MKISSRLKTYMIWLFWAVLWQAAHLAIQNDVIFVGPFDMLGALW
                     QLLPEVSFWQTLLHSFFKISAGFLMAFVLGILLGWASYACSFVKDLLEPPMLLIRSVP
                     VASFVILALIWIGSDGLSVFISFLVVIPMIYTGTLTGLEHTDRELLEMSRVFSFSFFK
                     RLRYIYIPAVHPYLLSSCRTALGMSWKSGVAAEVIGIPQASIGEQLYYSKLYLDTAGL
                     FAWTFAIIVISAVFERFFLSLLMRVRH"
     gene            complement(2924..4039)
                     /locus_tag="ACZPCG_05125"
     CDS             complement(2924..4039)
                     /locus_tag="ACZPCG_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1284492.1"
                     /translation="MKKQISVLTALLTAAAFAVTGCGAAGDNAAPSASSKAASQEVPE
                     ETEHLEAESTEAENTGSEAVSEEEKEGSGASAVRVGSLKGPTSMGLAYLMETAKNGET
                     TNTYEFTMAGKADELVGNIANGSLDIALVPANVASVLYNKTQGQVKVIDINTLGVLYV
                     VASDDSISTMEDLKGKTVYMTGKGTTPEFAMNYLLAENGLGSGDVTMEFKSEAAEVAS
                     ILKEDPSAIGVLPQPFASAACMQNPELKTVMDLTEQWDLLNKEGNSRMVTGVTIVRTG
                     FLEEHEAAVERFLKDHAESAEYTSAEPEKAAQLVADIGIVEKAAMAQKAMPLCNIVCI
                     TGEEMKQALSGYLEVLSGQDAKSIGGTLPGDDFYYLP"
     gene            complement(4240..5967)
                     /locus_tag="ACZPCG_05130"
     CDS             complement(4240..5967)
                     /locus_tag="ACZPCG_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HIR1284493.1"
                     /translation="MNTLVIVMIAAVCLLAGYTLYGRWLAEKWGIDPKAKTPACALED
                     GQDYVPTDGWTVFAHQFSSIAGAGPVTGAIQAAAFGWLPVLLWVILGGIFFGAVTDFG
                     ALYASVKNEGKSMGLLIERYIGKTGRKLFLLFCWLFTLIVIAAFADMVAGTFNAYTVA
                     DGAAVLSPAAQTNGAAGSISLLFIVFAMIFGVIQKKLQLKGWKKTAAGLACTAAALGI
                     GMAFPLAAGKDTWSYLTFAYIFMAAVLPMWLLMQPRDYMTTFMFAGMIIGAAAGLAVA
                     HPSMNLPAYTGFKNASMGDMFPILFVTVACGAVSGFHSLVSSGTSSKTISNEKDMLKV
                     GYGAMVLESLLAVLALCVAGAAAGADGTPAVGTPFQIFSAGVAGFLEMFGIPMYVAQC
                     FMTMCVSAPALTSLDSVARIGRMSFQELFSTDDMEQAEHWRQVVCNKYFATMITLACG
                     FILTKIGYSNIWPLFGSANQLLSALVLITLCVFLKVTGRENRTLFPPLIIMLCVTFTA
                     LIERSIALVHAFGAGSAVFMVEGLQLIIAALLMVLGVIIVYTSGRELFRKKEVREKSV
                     GKTGIQAGA"
     gene            complement(6072..6515)
                     /locus_tag="ACZPCG_05135"
     CDS             complement(6072..6515)
                     /locus_tag="ACZPCG_05135"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012198937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA];
                     GO_process: GO:0016075 - rRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HIR1284494.1"
                     /translation="MERNSDWNGNRQTYRPVIRGDLFYADLSPVRGSEQGGIRPVLVI
                     QNDTGNRHSPTVIVAAITSRTTKAAIPTHVSLDKTLCGLKQDSTVLTEQIRTIDRSRL
                     KEYIGHLDRRQMGGVDRAMAASLGLEWYFRSGRNMSGSYEIMAQT"
     gene            complement(6666..8147)
                     /locus_tag="ACZPCG_05140"
     CDS             complement(6666..8147)
                     /locus_tag="ACZPCG_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR1284495.1"
                     /translation="MEANTLQEIISRAADSYGSQTAFRYKVKRELEEKTYEDLHRDTM
                     AFGRMLEEKGMRGKHIAVIGPTSYGWITSYFGAANSKSVAVPIDVQLPAEAVCELLNR
                     ADVEMLVFDEVRTDVAEAAASFCPKIREFLSIQSLPELLEAYRGEFHCEVKPEDLCTI
                     LFTSGTTGKSKGVMLTHRNLADNALCLDMKIPQGTVSLTVLPINHVYCLTMDIIKGIS
                     IGMIICINDSIMRFQRNLKLFQPEIVLLVPMIIESLAGKLKEIDPSVPKEQAAAAIFG
                     GKLKTICSGGAYLDPDCIRLFKEFGITILQGYGMTECSPVISTNLEWENKIGSVGKLL
                     PNCEAKTVDYELWVRGSSVMQGYYKMPEETAAALEDGWLKTGDLGYVDEDRFVFLTGR
                     RKNLIILANGENVSPEELENELGRNDLVKEILVREHEKVIEAEIFPDPEYMEKHTITE
                     PEPLLQELIDRLNGSMPVYKRIARLIVREVPFERTAAGKIKRF"
     gene            complement(8172..8405)
                     /locus_tag="ACZPCG_05145"
     CDS             complement(8172..8405)
                     /locus_tag="ACZPCG_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR1284496.1"
                     /translation="MFEELKEMICEYVDVEPGEIREDSRFVEDLGFNSYDFMSMVGEI
                     EEHWDVEVDEREVVNVKTVKDAMEYIQSLQQAV"
     gene            complement(8425..9840)
                     /locus_tag="ACZPCG_05150"
     CDS             complement(8425..9840)
                     /locus_tag="ACZPCG_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HIR1284497.1"
                     /translation="MKTRRGCKVYPLTEAQKLHFYCLKYCPQKQVLNIGSSLTIEIDL
                     DWEVLRESIKEAISRCEGMRIRFAHDKKEGKEYQYVVKEDHSEIGHFDFCGWKMEDAE
                     AKMREWTELPFERYDSPMHRIVMIRLPNGFQGTYVCVDHMTMDAQSLILFYRDIIEIY
                     SHKMFGDEVIAYPKAMTSYIKQLEKDLAYEAGSTSSQRDREFFEKLIGTSEPIFNSIY
                     GPGLLEQQRKASGNPDQRAARTIPEDVSANITTFRLEPEPSERLLTFCEEHRISMTCL
                     LLMGLRTYLQKQNGNEDVSLTTTIARRATLTEKRCGGTRIHCFPFRTIISRDKTFLEG
                     MEIIRDTQNQYFRHASYSPTEYFNYRKKFYNLEDGLTYEPLALTYQPLAMKYEGPGLD
                     KMGGIRYDTARYSNGVAGQPLYLTVSHRALDDGLDFCFEYQTGIVTPQKLEEVYYYLC
                     RIIFRGVEDCSRTVGEIIDMV"
     gene            complement(9837..10805)
                     /locus_tag="ACZPCG_05155"
     CDS             complement(9837..10805)
                     /locus_tag="ACZPCG_05155"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR1284498.1"
                     /translation="MKIKWMLGMGAGAAALLAAGEYALARYFLRRTLMRGKADNKRTQ
                     KMAGTDWSVYLPFMEENRQWFLSQPHEDVYIQSEDGLKLHGTYFPGKDPKRAVICFHG
                     YTSRGLNDYPSLARFYMGCGLGVLTVDERAHGDSEGIYIGFGCLDRLDAKKWMEYMAG
                     RLGEDCELLLQGISMGAATVDMAVGLELPPQVKGAVSDCAFTSAKEVFTSVLKTTYHM
                     PAFPLMDLSDRMAKKEAGYGLDECNARDEVKKAKIPMLFIHGSKDTFVPCSMVHELYE
                     ACAAPKDLLIIEGASHAEAYYKDRPAYEAAVRKLMERAFTKGVCEE"
     gene            complement(10802..11431)
                     /locus_tag="ACZPCG_05160"
     CDS             complement(10802..11431)
                     /locus_tag="ACZPCG_05160"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase family
                     protein"
                     /protein_id="HIR1284499.1"
                     /translation="MIYAAAYRPEGRKCDRSREHILGRRLLERGLLQEYGRTWEVIAP
                     ENGKPFLKDSPGVFFSISHTEGMAVCAVCSHPVGIDIQVRKPCSKGLVRRICSPEEYR
                     WICEKKEKEAEKTALRLWTLKESYAKADGRGLAFPFRQISFANRLEREPECIFYGTAS
                     FELPLDLGDRHGFTADIPGWRFFEAQLGDAYHIGVCMRDTDPDKKGAAG"
     gene            11623..12240
                     /locus_tag="ACZPCG_05165"
     CDS             11623..12240
                     /locus_tag="ACZPCG_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR1284500.1"
                     /translation="MQYLNKREKILDAMQQLMTTADTRSITVSEIAQKAGIGKGSIYY
                     YFPSKNDILDGVIERAYSRVLEKGKALAAASDIDAFCKMEIIYHACLEASKELRRQES
                     FNAAPDGQERAFIHLKFAKAIITHLRPILADILRQAVAENGAVCEYPEETAYIILLVL
                     TATLDNRLVPMKESDVSRILTAFTQMQGQGLNIPPEVLHFLIREV"
     gene            12245..15052
                     /locus_tag="ACZPCG_05170"
     CDS             12245..15052
                     /locus_tag="ACZPCG_05170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR1284501.1"
                     /translation="MNINKKQKLQTPGSRRRLVTALGITGWLFKGVLSVFTAAFLIMA
                     AAGLILYAAVKPELDKCREIAYDKLAQMDRSDFSMLSDTVIYDKDGKQIGLINAGHYQ
                     YMDINHISMNLQNGYIAQEDRRFKNHNGVDWIATFRAGLALIKHGGEVTQGGSTITQQ
                     VIKNTYLTQERTFTRKIVEILLAPELEKKYSKADIIEFYCNTNFYGHRCYGVEAASLY
                     YFGKHAEDLAPEEAAVLIGISNSPSAYDPVSHPNASKNKRDDVLKSMNEVGYLSNEDY
                     EKAVSSPLKIVQKETEGTDENYQSSYAIHCAALELMKMDGFEFQYTFDNKEDYTLYSE
                     RYTTAYSEQSDRIRAGGFQIYTSLDSGLQAVLQTQIDASLSGFTELQENGKFALQGAG
                     VIVDNKINYVTAIVGGRGADDPFNRAYLSARQPGSTIKPLIDYGPAFDTGEYYPTRMV
                     NDHKWEKGPSNSGRRYFGNVTVREALNRSLNTVAWQILEDIGIDFGLSYLGEMEFQKL
                     SYVDNQVPSLSIGGFTNGVRVVDMAKGYSTLANGGVYNDRTCIVKILHEKEGELTKDM
                     TPFAKQVYRSDSAFMLTDVLKGTFTSPYGTGRGLGLENDMPAAGKTGTTNSSKDTWFC
                     GYTPYYTAAVWVGYDIPRNMPGIYGATYAGKIWKNVMDEIHQGLPPLDWEQPDTVERR
                     ADETNGIEDYFSTTAQFRAQQSLHEKEQAALLEELTQEAEAFCAREIRSVEDTYTVKD
                     EYASITSRLPLLDDGEQRALLLEQTEQRYDYFVSIISQMGDEIRRYEEQKAAEEKKAR
                     EEAAKEAEKQRLEEDKVTKKNSFLLALETVEELEYQREDAERLVNDAIDKLSLIAGDP
                     EANALSDRLQTAISRISTLPTAEEWEKLQAQEAEQEAKKEQEAQLEIQSSQARLRSSL
                     SRERFKWNNMEYYGPGGRGDE"
     gene            15045..15359
                     /locus_tag="ACZPCG_05175"
     CDS             15045..15359
                     /locus_tag="ACZPCG_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284502.1"
                     /translation="MNKHNTASMQNGKKRPSFSILPEKPDGAEDWFYTFMCMNIPFYG
                     WRYLKQLVYQEHNDSPKSQFARAYLYYKRVFLRISLAIAALLAVIGFAGLHLLIRYME
                     ML"
     gene            complement(15442..15978)
                     /locus_tag="ACZPCG_05180"
                     /pseudo
     CDS             complement(15442..15978)
                     /locus_tag="ACZPCG_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577539.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
     gene            complement(15999..16580)
                     /locus_tag="ACZPCG_05185"
     CDS             complement(15999..16580)
                     /locus_tag="ACZPCG_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIR1284503.1"
                     /translation="MSKEYWKPGNMLYPVPAVMVSCAREGETPNIITVAWAGTVCSDP
                     AMVSISVRKERFSHRIIKDTGEFVINLVNRPLVRATDYCGVKSGRDVDKFKEMRLTPQ
                     PSRYIKAPGIAESPVNIECKVTEVKELGSHDLFLAQVVGVTIDNQYMDAKGKFNLNSS
                     GLVSYSHGEYFELGKKLGSFGYSVKKPVKKKKR"
     gene            complement(16600..>17762)
                     /locus_tag="ACZPCG_05190"
     CDS             complement(16600..>17762)
                     /locus_tag="ACZPCG_05190"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HIR1284504.1"
                     /translation="IAAANPDLMEFYIEPHDLVILGNRYESQLCAIEMGADCIIVCEG
                     AGVSMTIKKIAQERGCTIIATTYDTYTAARLINQSMPISYFMTREHLITFESDDYIDE
                     IRDVMANKRHRDFPILDKDGRYLGMISRRNLLGAKGKRVVLVDHNEKNQAVAGIENAE
                     IMEIIDHHRLGTIQTMSPVFFRNQPLGCTATIIYQMYQEAGVKVEPKIAGLLCSAIVS
                     DTLLFRSPTCTPMDEMAARSLAEIAGIEIEKYAMEMFGAGSNLKGKSDEEIFYQDFKR
                     FTSGKIAIGVGQITSLNGDELESIRPRVIHYMENARQENSLDLVFFMLTNILTESTDL
                     ICEGNGALQLAEKAFHTEPDESGEQTEPLVRLPGVVSRKKQLIPALMLAEQE"
BASE COUNT         4518 a         4925 c         3972 g         4347 t
ORIGIN      
        1 ctgcatccgc agccgcatct gttttggcat ccgcagccac atctgttttg acatccacag
       61 ccagagccat ttccattctg ttctgcattt ctgcatccat tattacaaca gcataaaaga
      121 caacacataa tctctactct ccttttttaa ttggttgtac catatcatat gggacagtga
      181 gacaaagtgt tcctgcgcaa aaaccgtcac ctgttatgta tctcttcata aaataataaa
      241 gaaagaataa aaaggagatt tcaaacaatg gaagcatata tggatgcaaa agcttcgacc
      301 tgcagctgcg gatgcggatg cagaagccgt gtcgttcccg gcgtatcaga cagaccggcc
      361 gcacagccgg aatattccgg accatccccc cagatgtggc ccggatggga tatgaatcag
      421 tcccttcctt atggagaagc cggatatccc cagacaggat atcctcagat aggaacaaat
      481 atgaatcagg gaagtaccgt atggcccatc gtgaactgcc cggataccac agccggttat
      541 ctggaacagc atcaccccgt agctatggcc tatgtaccgt ggcagcagtg gcagactgcc
      601 tatgcgcctg aacgcggcct catgcaggga accatcttcc ctgacctgga tcttccattt
      661 aattacggga ggtgcggacg atgacagaac atcttcagaa cccatcaaga ggagccatgc
      721 tccaggcagt aaaccagacc agctttgccg tagtggaggc caatctctat ctggacactc
      781 atccctgtga tatggaagct ctctcctatc tcaatcagat gatggaagcc tacaaaaacg
      841 caaaagccgc ctatgaggcc cggtttggcc ccttaaatgc gggcaatgtg actgaaaatg
      901 cctactggac ctgggtcagt gatccctggc catgggaagg agggaattta taatgtggat
      961 ttatgaaaaa cgtctccagt ttccggtaaa tattaaggag ccgaatgcca aactggcgca
     1021 gtttattatg agtcaatacg gcggccccga cggcgagatg ggcgcatcca tgcgctatct
     1081 gtcccagcgc tacgccatgc cttataaaga aggcaaggct gttttgaccg atatcggcac
     1141 agaggaacta gcacatctgg aaatgatcgc cgccattgtc catcagctga cccggaacct
     1201 cacagccgag cagctggtag aacagggctt cggaccctat tatattgacc atgccaccgg
     1261 aatctggcct caggccgcag gcggcattcc ctttaatgcc tgcgaattcc agtccaaggg
     1321 cgatgtgatc acagaccttc atgaagatat ggcagcagaa caaaaagccc ggagtaccta
     1381 tgacaacatc ctccgggtag taacagacta tgacgtatgc gaacctatca agttcctgag
     1441 agaacgtgag atcgtccatt accagcggtt cggtgaaacc ctccgaatca cccaggaaaa
     1501 actggacagc cggaatttct atgcctttaa tccggccttt gatccggatc aggcgcccca
     1561 gccccgataa acagccctta atccagctgt atccggactc cgccaaggag ctttacgtct
     1621 tcctcctgat gggttgtgat caggaggaag cggttccggc ggttttccag aatatagcgg
     1681 attacctcca gcttaagggc ctcatcaagc ccggtaaagg gttcatccat tacaagcata
     1741 tgggaaggcg caggaacggc ccgcagaatg gcggtgcgcc gcttcatccc tccgctcaag
     1801 gtggacacgg ggcgggtaag gcattcttta ggaagtaagc aggtcatggc ctgagccagt
     1861 ttttcttcct tccatttttc ttttaatgcc atctgcacat tttccatagg ggaaaaggaa
     1921 ggacagagac gatcctcctg aaaaactgcc gaaatcctca aaggccaccc agggcccccc
     1981 tgagtaaaaa tccttccgct gtcagccgtt tcaagcccca tgaggatccg aagaaaggtt
     2041 gtttttcctg agcctgaggg cgacatcagg caataacagg catcctgctc cagcgtcagg
     2101 ctcagatccc gaaggacctg cagttctccg tattttttag aaagatgctc gatgcaaagc
     2161 ttcatacaac ctcctcatca gtggcgtacc cgcataagca ggctcagaaa aaagcgctca
     2221 aagacagcgc tgatcacaat aatggcaaag gtccaggcaa acaggcccgc tgtatccaga
     2281 tagagcttgg aatagtagag ctgctctccg atcgacgcct gaggtatgcc gatcacctct
     2341 gccgcaactc ctgatttcca gctcatccca agagccgtac ggcagctgct taaaagatag
     2401 gggtgaacgg ccggaatata tatatatcga aggcgtttga aaaaggaaaa cgaaaaaact
     2461 cggctcattt ccaaaagctc cctgtccgta tgttccagac cggtcagcgt tcccgtatag
     2521 atcataggga tcacaaccag aaatgaaata aacacggaca ggccgtcaga gccgatccag
     2581 atcagtgcca ggatcacaaa ggaggccacg ggaacggaac ggatcagcag catgggaggt
     2641 tccagaagat cctttacaaa ggaacaggcg taactggccc accccaggag aatccccaga
     2701 acaaaagcca tgagaaagcc ggcgctgatc ttaaaaaatg aatgaagcag cgtctgccag
     2761 aaggaaactt ccggtaaaag ctgccaaagg gccccaagca tatcgaaggg ccccacaaaa
     2821 atcacatcat tctggatggc taagtgggcg gcctgccaga gaaccgccca gaaaagccat
     2881 atcatatatg tttttaaacg cgaagatatt ttcatgtttt tcattatggc agatagtaga
     2941 agtcatctcc gggaagcgtt ccgccgatgg acttggcatc ctgtccggaa agcacctcca
     3001 gatagccgga aagggcctgt ttcatttctt caccggtaat gcatacaata ttgcagagag
     3061 gcatcgcttt ctgggccata gcagcctttt ctacgattcc aatatcagct accagctgtg
     3121 cggccttttc aggctcagca gatgtatact ccgcagattc tgcatgatcc tttaaaaacc
     3181 gctccacagc agcctcatgc tcctctaaaa atccggtgcg gacaatggta actcccgtaa
     3241 ccatccggct gtttccttcc ttatttaaaa gatcccactg ctcggtcaga tccatcactg
     3301 ttttcagctc cggattctgc atacaggccg ctgaagcaaa gggctgaggg agcacgccga
     3361 tggcagacgg atcttccttt aatatagaag caacctctgc agcctcggat ttaaactcca
     3421 tggttacatc cccggagcca aggccgtttt cagccagaag ataattcatg gcaaattccg
     3481 gcgtggttcc ttttcctgtc atatatacgg ttttaccctt caaatcttcc attgtggaaa
     3541 tggaatcatc cgaagccact acatacaaaa ctcccagtgt attgatatca atgaccttta
     3601 cctgcccctg cgtcttattg taaagcacgc tggctacatt ggcaggcacc agcgcaatat
     3661 ccaggcttcc gtttgcaata tttcctacca gctcatctgc cttccctgcc atggtaaatt
     3721 catatgtatt ggtagtttct ccgtttttgg cagtttccat cagataggcc agtcccatag
     3781 aggtaggtcc ctttaaggag cccacacgga cagcggatgc gccggaacct tccttctcct
     3841 cttctgaaac cgcctcagaa ccggtatttt ctgcctcagt ggattctgcc tctaaatgtt
     3901 ccgtttcctc tggaacctcc tgagaagctg ccttggaaga agcggaagga gccgcgttat
     3961 cgccagctgc gccgcagccg gttacagcga aggctgctgc agttaagagt gcagtcaata
     4021 cagaaatttg ttttttcaca gtacattctc ctctctggca ccctctggat gccatccctt
     4081 actatagcac attttttcct gtttttccat cctcaccgcc ggaaaaacag aatcgcctcc
     4141 agcgttcgaa cagctcaaat ccataaaaac ggacggccct caacgggatt ccgtccgttt
     4201 ttcttatttt cttaaatact catttttccc ctggatcatt tatgcgcctg cctgaatacc
     4261 ggtctttccc acgctctttt cccgaacctc ctttttccga aacagctcac gaccggaggt
     4321 atacacgatg atcacaccga gaaccattaa aagagcggca atgatcagct gcagtccctc
     4381 taccataaat acggcgcttc ctgcgccaaa agcatgaaca agtgcaatgg aacgctcaat
     4441 taaagcggta aaggtaacgc agagcatgat aataagcgga gggaacagcg ttctgttttc
     4501 tctgcccgta acctttaaaa agacgcacag cgtaatgaga accagagcac tcaaaagctg
     4561 attggcgctt ccaaaaagag gccagatatt ggaatatccg atttttgtca ggataaagcc
     4621 gcaggctaat gtgatcatag ttgcaaaata tttattgcat accacctgcc tccagtgctc
     4681 cgcctgttcc atatcatctg tactgaacag ctcctgaaag gacatacggc cgattcttgc
     4741 aacggaatca aggcttgtaa gagccggagc tgatacacac atggtcataa agcactgggc
     4801 tacatacata gggattccaa acatttccag aaatcctgcc acaccggctg aaaagatctg
     4861 gaagggagta cccacagcag gcgttccgtc tgctcctgca gcagcgcctg ccacacacag
     4921 ggccaaaact gccagaagac tttccagaac cattgctcca tatcccactt tcagcatatc
     4981 cttttcattg gaaatggtct tggatgaagt tcctgatgat accaggctgt gaaatccgga
     5041 tacagctccg caggctaccg taacaaacag aataggaaac atatctccca tggatgcatt
     5101 tttaaagcct gtgtatgcag ggaggttcat ggacggatgc gccactgcca gacccgccgc
     5161 agcaccaatg atcatgccgg caaacataaa ggttgtcata taatccctgg gctgcatcag
     5221 aagccacatg gggagcacgg ctgccatgaa gatatatgca aaggtcagat aggaccaggt
     5281 atcctttcct gctgccagcg gaaatgccat tccaatccca agagcagccg ccgtacaggc
     5341 cagtcccgct gccgttttct tccagccctt taactgtagt tttttctgga tcactccaaa
     5401 tatcatcgca aatacaataa acagaaggga aatacttcct gccgctccat tggtctgtgc
     5461 ggccggagaa agaactgccg ctccgtcagc cacggtatat gcgttaaacg tacctgccac
     5521 catatctgca aaagctgcga ttacgatcag ggtaaaaagc cagcaaaata aaagaaacag
     5581 ttttctccct gtctttccaa tataccgctc gatcagaagc cccatggact ttccttcatt
     5641 ctttacactg gcatacagtg ctccgaaatc cgtaactgct ccaaagaaaa tgcctcccag
     5701 gatcacccac aaaagcacgg gcagccatcc aaaagcagcg gcctggatcg ctccggtcac
     5761 agggccggcc cccgcaatgg atgaaaactg gtgggcgaat accgtccatc cgtctgtagg
     5821 cacataatcc tgtccgtctt ccagtgcaca ggcaggcgtt ttggccttgg gatctatgcc
     5881 ccatttttct gccagccaac ggccatacag ggtatatccc gccaaaagac ataccgctgc
     5941 gatcattaca attacaagtg tgttcatact cttatctcct tccttctgga ataactgtca
     6001 ttataatccc ttttattcgg aagtcaatca gatagaacat accgtatttt catatttttt
     6061 attctttcag gttatgtttg tgccataatc tcataactgc cagacatatt tctgccagac
     6121 cggaaatacc attccagccc caggctagct gccatggcgc ggtccacacc gcccatctgc
     6181 cgccggtcca ggtgccctat gtattccttt aagcggctgc ggtcaatggt gcggatctgc
     6241 tctgtaagca ccgtagaatc ctgttttagt ccgcacaatg tcttatccag gcttacatgg
     6301 gtaggaatcg ccgccttggt ggttcggctg gtaatggcag ctacgatcac cgtcgggctg
     6361 tggcggtttc ccgtgtcatt ctggatgact aaaactggcc ggatccctcc ctgctccgat
     6421 cccctgaccg gtgaaaggtc tgcataaaag aggtctcccc ggatcaccgg gcggtacgtc
     6481 tgtctatttc cattccagtc tgaatttcgt tccatggctt gtctccttat tttaagtttc
     6541 atcacttcat tcatacactc ataggattcc caattccgga gatttcaaac atttcctgaa
     6601 aaaaacatga aaaagcccca tcctcagagg acaaggccat tcattttcct ttattctgtt
     6661 ttgctttaaa accgcttgat ttttcctgcg gctgttcgtt caaaaggtac ttctctcacg
     6721 atcagcctgg caatgcgctt atagacaggc atagacccat tcaggcggtc aatcagctcc
     6781 tgaagaagcg gctctggctc tgtaatcgta tgcttttcca tatattccgg atccgggaag
     6841 atctctgcct cgatgacctt ctcatgctcg cgcaccagaa tctcttttac cagatcattg
     6901 cgtcccagct cattttccag ctcctcagga gatacatttt ccccgtttgc aaggatgatc
     6961 aggttctttc tgcggccggt aagaaataca aagcgatcct cgtccacata gcctaagtct
     7021 cctgtcttta accagccgtc ctccagagcg gcagccgttt cctcaggcat cttgtaatat
     7081 ccctgcatca cgctgcttcc ccgtacccag agctcgtaat ccacggtctt tgcctcgcag
     7141 tttggcagaa gttttcctac ggatccgatc ttattttccc actccagatt ggtgctgatc
     7201 acaggagaac actccgtcat gccgtatccc tgaagaatgg tgatgccaaa ctccttaaaa
     7261 agacggatac agtccggatc gaggtaagcg cctccgctgc agatggtctt aagctttccg
     7321 ccgaatatgg ccgctgcagc ctgttccttt ggaacagacg gatcgatctc ctttaatttt
     7381 cctgccagcg attcgatgat cataggcacc aaaagcacga tttccggctg gaacagcttt
     7441 aaatttctct ggaaccgcat gatcgaatca ttgatgcaga tgatcattcc aatggaaatg
     7501 ccctttataa tgtccatggt cagacagtac acatggttaa tgggaagaac ggtcagggat
     7561 acggttccct gagggatctt catatccaga cacagggcat tgtccgccag attccggtgg
     7621 gtcagcataa cgcccttgct ctttcctgtg gttcctgagg taaaaagaat ggtacacaga
     7681 tcctccggtt ttacctcaca gtgaaattct ccccggtatg cctctaaaag ctcgggcaga
     7741 gactggatgg aaagaaactc acggatcttt ggacagaaag aagcggccgc ctctgccaca
     7801 tccgtcctca cctcatcaaa caccagcatt tccacatctg cccggtttag gagttcacag
     7861 actgcctctg caggaagctg tacatcaatg ggaactgcca cacttttact gtttgccgcg
     7921 ccaaaatatg aagtgatcca tccatagctg gtagggccga tgacagcaat atgctttcct
     7981 ctcatcccct tttcttccag catacgtcca aaggccatgg tatccctgtg cagatcttca
     8041 taggtctttt cctcaagctc ccgctttacc ttatagcgga acgcggtctg gctgccgtaa
     8101 gaatcagccg cccggcttat gatctcctgt aatgtatttg cttccattcc ttctccttcc
     8161 tcgttcccgc ctcagacagc ctgctgcagg gactggatat actccatggc gtcctttact
     8221 gtctttacat tgaccacttc ccgctcgtcc acttcaacgt cccagtgctc ctcgatctct
     8281 ccgaccatac tcataaaatc ataggaatta aagcccaagt cctccacaaa acgggaatcc
     8341 tcccggattt ctcccggttc tacatctaca tactcacaga tcatctcttt taactcttca
     8401 aacatatcct ttttctccct ctttttatac catgtcaata atctctccta ccgtcctgct
     8461 gcagtcctcc acacctctga aaatgatcct gcacagatag tagtatacct cttccagttt
     8521 ctggggcgtc acaataccgg tctggtactc aaagcagaaa tccagaccat cgtccagagc
     8581 gcggtggctc acagtcagat acagcggctg tcctgccaca ccattggagt aacgggctgt
     8641 atcatagcgg atcccgccca tcttatcaag gcccggcccc tcatatttca tagccagagg
     8701 ctgataggtc agagccaggg gctcataggt caggccgtct tccaggttat aaaatttctt
     8761 tctgtaatta aaatactccg tagggctgta gcttgcgtgc cggaaatact gattctgtgt
     8821 atcccggatg atctccattc cctctaagaa ggtcttgtcc cgtgaaataa tggtcctaaa
     8881 ggggaagcag tggatcctgg taccgccgca tcgcttctcg gtcagagtgg cccttcttgc
     8941 aatagtggtc gtaagagaaa catcctcatt tccgttctgc ttctgaagat aggtcctaag
     9001 gcccatgagc agaaggcagg tcatggagat cctgtgttcc tcacagaacg ttaaaagccg
     9061 ttccgacggc tccggttcca gccggaaagt ggtaatattg gctgatacat cctccggaat
     9121 ggtgcgggcc gccctctgat ccgggttgcc ggaagccttt ctctgctgtt caagaagccc
     9181 tggaccgtag atgctgttaa agatcggctc cgatgtgccg atcagctttt caaaaaattc
     9241 ccggtctctc tggctggatg tacttcccgc ctcataagcc agatcctttt ccagctgctt
     9301 tatgtaagag gtcatggcct ttggataagc aatcacctcg tctccaaaca tcttatggct
     9361 gtagatttca atgatatccc tgtaaaacag gatcagggac tgggcgtcca tggtcatgtg
     9421 gtctacgcac acataggttc cctgaaagcc gtttggaagg cggatcatca caatgcggtg
     9481 catgggagaa tcgtaacgct caaagggaag ctcggtccac tccctcatct tcgcctctgc
     9541 atcctccatt ttccagccgc aaaagtcaaa gtgtccgatc tcagagtgat cctcctttac
     9601 cacatactgg tactcttttc cctctttttt gtcatgggca aaacggatcc gcattccctc
     9661 gcagcgggaa atcgcttcct taatgctctc ccgcagcacc tcccagtcca gatcgatctc
     9721 aatagtcagg ctggagccga tatttaatac ctgcttttgg ggacagtatt tcagacagta
     9781 aaaatgcagc ttctgcgcct cggtcagcgg atatacctta cagccccttc tcgttttcat
     9841 tcctcacata cccctttcgt aaatgcacgt tccatcagtt tccttacagc cgcctcataa
     9901 gccggacgat ccttataata tgcctctgca tgggaagctc cctctataat cagaagatcc
     9961 tttggagcag cgcaggcttc gtagagctca tgcaccatag agcagggcac aaaggtatcc
    10021 tttgatccgt ggataaacag catggggatc ttagcctttt ttacctcatc tctggcattg
    10081 cactcatcca ggccatatcc ggcttccttt tttgccatcc ggtcggacag atccattaag
    10141 ggaaatgccg gcatatgata ggttgtctta agaacagagg taaatacctc tttggcggag
    10201 gtaaaggcgc agtcggaaac agcccccttt acctgcggcg gaagctcaag tcctactgcc
    10261 atatccaccg tagcggctcc catggaaatg ccctgaagca gaagctcaca gtcctctccc
    10321 agacgccctg ccatgtattc catccatttt tttgcatcca gacggtccag acagccaaaa
    10381 ccgatataga taccctcgct gtctccatgg gctctctcat ccaccgtcag gacgcccagc
    10441 ccgcatccca tataaaaccg ggccagggac gggtaatcat tcagtccccg gctggtatag
    10501 ccatgaaagc agatcacggc ccgtttggga tcctttcccg gaaagtatgt tccgtgaagc
    10561 tttaagccat cctcagactg tatgtacaca tcctcatggg gctggcttaa aaaccactgg
    10621 cggttttcct ccataaaggg cagatagacg ctccagtccg tacctgccat tttctgggtc
    10681 cgcttattat ccgcctttcc ccgcatcagc gtccggcgca gaaaatagcg cgccagcgca
    10741 tactctcccg cagcaagcaa agctgccgct ccggctccca ttcccaacat ccactttatt
    10801 ttcatcctgc cgctcctttt ttatccggat ccgtatccct catacagacc cctatatgat
    10861 acgcatctcc cagctgggcc tcaaaaaagc gccagccggg gatatctgct gtaaaaccgt
    10921 ggcgatctcc cagatccaga ggaagttcaa aggaagctgt cccataaaaa atacactccg
    10981 gctctctttc cagcctgttt gcaaatgaaa tctgccgaaa gggaaaggca agtcccctgc
    11041 cgtctgcctt tgcatagctt tcctttaacg tccacaaacg gagcgcagtt ttctctgcct
    11101 ctttctcctt cttttcacaa atccatctat attcttccgg agaacatatc ctcctgacca
    11161 gccctttgct gcagggcttt cggacctgga tatcaatccc tactggatgg ctgcagaccg
    11221 cacagacggc cattccctct gtatggctga tactaaaaaa aactcctgga ctgtctttta
    11281 gaaagggttt tccattttcc ggagctatca cctcccaggt tctcccatat tcctgcaaaa
    11341 gcccccgctc aagaagcctt cttcccaaga tatgttctct ggaccggtcg catttcctgc
    11401 cctccggccg gtatgcggct gcgtagatca tactccccct cctgctgcgt tcccatggtt
    11461 ttccctggtt tttccgtgtt ttcgaccgct agtcgaatcg tttctgcctt acaatatcat
    11521 attttcaacc agtggtcaag tgtggatttt ttatatcaca tatgctatac tcgaaccatt
    11581 ctgaaaacca tccatatgac actaaaagaa agcaggaaca tcatgcagta tctgaataaa
    11641 cgcgaaaaaa tcctggatgc catgcagcag ctgatgacaa ccgctgatac ccgttccatt
    11701 acggtcagtg agatcgctca gaaagccggc attggaaaag gaagcatcta ttattatttt
    11761 ccctctaaaa atgatattct ggacggagtc attgagcggg cctattcccg cgttctggaa
    11821 aagggaaagg ctctggccgc tgcctcagac atagatgcct tctgtaaaat ggagatcatc
    11881 tatcacgcct gtctggaagc ctctaaggag ctgcgccgtc aggagtcctt taacgccgcc
    11941 cccgacggcc aggagcgggc cttcatccat ctgaaatttg ccaaagccat tatcacccat
    12001 ttaagaccta ttcttgcaga tatcctccgg caggctgtgg cagaaaacgg tgcggtctgc
    12061 gaatatccgg aggaaacagc ctatatcatc cttctggtat taaccgccac tctggacaac
    12121 cgtctggtac ccatgaagga aagcgacgta agccggattc tcactgcatt tacccagatg
    12181 cagggccagg ggctcaatat tccgccggag gttctgcatt ttctgatccg ggaggtatag
    12241 cgccatgaat attaataaga agcaaaaact ccagactccc ggctcacgcc gccgtctggt
    12301 tacggctcta ggaatcaccg gctggctttt taaaggagtt ctttctgtct ttaccgctgc
    12361 ctttcttatc atggcggcag caggactgat cctctacgca gcagtcaaac cggagcttga
    12421 caaatgccgg gagattgcct atgacaagct tgctcagatg gaccgttccg atttttccat
    12481 gctgtccgat acagtaatct acgataagga cggaaaacag atcggtctga tcaacgcggg
    12541 tcattatcag tatatggata ttaaccacat cagcatgaac ctgcagaacg gttatatcgc
    12601 ccaggaggac cggagattta aaaaccataa cggcgtagac tggatcgcca ccttccgggc
    12661 cggtcttgcc ctgatcaagc atggcgggga agtgactcag ggcggcagta ccattaccca
    12721 gcaggtgatc aaaaatacat atctgaccca ggagcgcacc tttacccgca agattgtgga
    12781 aatcctgctg gctcccgaac tggaaaagaa atactccaaa gccgatatca tagagtttta
    12841 ctgcaacacg aatttctacg ggcaccgctg ctacggtgtt gaagctgcca gtctgtatta
    12901 ctttggaaaa cacgccgaag atctggcccc tgaggaagcg gctgtgctga tcggcatcag
    12961 caacagcccc tctgcctatg acccggtttc ccatccgaac gcttccaaaa acaaacggga
    13021 tgatgtatta aaaagcatga acgaggtagg gtatctgtca aatgaagact atgaaaaagc
    13081 cgtctcttct cctttaaaaa tcgtacagaa ggaaacagag ggcacggatg aaaattatca
    13141 gagcagctat gccattcact gcgctgccct ggagctgatg aaaatggacg gttttgaatt
    13201 tcagtatacc tttgataaca aagaagatta caccctttac tccgaacgct atactacagc
    13261 ctactccgag cagtcagacc ggatacgggc aggcggattt cagatctaca cctccttaga
    13321 cagcggactg caggctgtgc tccagacgca gatcgacgcc agtctttcgg gctttacaga
    13381 gcttcaggaa aacggaaaat ttgcccttca gggagccggt gtgatcgtag acaacaagat
    13441 caactatgta acggccattg taggaggacg cggggcggat gatcccttta accgggccta
    13501 tttaagcgcc agacagcccg gaagcaccat taagcccctc attgactacg gacccgcctt
    13561 tgacaccggg gaatactatc ccacgcgcat ggtcaatgac cacaaatggg agaagggtcc
    13621 ttccaacagc ggcaggcgct actttggcaa tgttaccgta agagaagcct taaaccgcag
    13681 cttaaacact gtggcctggc agatcttaga ggatatcggc attgatttcg gtctgagcta
    13741 tctgggagaa atggaatttc agaagctgag ctatgtggac aatcaggtgc cctccttaag
    13801 catcggaggc tttaccaacg gcgtccgggt agttgatatg gcaaagggct acagcacact
    13861 ggcaaacggc ggagtataca atgaccgcac ctgcatcgtg aaaatccttc atgaaaaaga
    13921 gggcgagctc acaaaggata tgacgccttt tgcaaagcag gtttaccgca gcgattctgc
    13981 ctttatgctc acagacgtct taaaaggcac atttacctcc ccctacggca ccggacgggg
    14041 acttggcctg gaaaatgata tgcccgctgc cggaaaaacc ggaaccacaa acagcagcaa
    14101 ggatacctgg ttctgcggct acacccctta ctacacagcc gccgtctggg tgggttacga
    14161 tatcccccgt aatatgccgg gaatctacgg agccacctac gcaggaaaaa tctggaaaaa
    14221 tgtaatggac gaaatccacc agggtctccc ccctcttgac tgggaacagc ccgataccgt
    14281 agagcggcgt gcagatgaaa ccaatgggat cgaagattat ttcagcacca cggctcagtt
    14341 ccgggctcag caaagccttc atgaaaagga acaggctgcg cttttagaag agctgaccca
    14401 ggaagcagag gcgttctgtg ccagagaaat ccgttctgtg gaagatacct atacggtaaa
    14461 ggatgaatat gcttctatca cttcccgtct ccccctgttg gacgacggag agcagcgggc
    14521 actgctctta gagcagacgg aacagcggta tgattacttt gtctccatca tcagccagat
    14581 gggtgatgaa atccggcgct atgaagagca gaaagctgcc gaagagaaaa aagccagaga
    14641 agaagctgcc aaagaggcag agaagcagcg tctggaagaa gacaaggtca ccaaaaagaa
    14701 cagcttcctt ctggcccttg aaacggtaga ggagctggaa taccagagag aggacgcgga
    14761 acgcctggta aacgatgcca ttgacaagct ttccctcatt gcaggagatc cggaagcaaa
    14821 tgctctctcc gaccggcttc agacagccat cagccgcatt tccacgcttc ccaccgcaga
    14881 agaatgggaa aagcttcagg cacaggaagc cgaacaggag gcgaaaaagg aacaggaggc
    14941 ccagctggaa atccagtcca gccaggcccg tctccgttcc tccttaagcc gggaacgctt
    15001 taagtggaac aatatggaat actatggacc aggaggacgg ggcgatgaat aaacataaca
    15061 cagcttccat gcaaaacggc aaaaaaaggc cctcattttc cattttaccg gaaaaaccgg
    15121 acggggctga agactggttc tataccttta tgtgcatgaa catccccttc tacggatggc
    15181 ggtacctaaa gcagcttgtt tatcaggagc acaatgacag ccccaaaagc cagtttgcca
    15241 gagcgtatct gtactataaa cgggtatttc tcaggatttc tctggcaatc gcagccctac
    15301 ttgctgtaat aggctttgcc gggctgcatc tgctgatccg atatatggaa atgctgtagc
    15361 acaaaaacag gcggcagact gccggagccc tcttctaaaa ggcccggcgt ctgccgcctg
    15421 tatgtatgta ctataaaatc atttctccaa agcagcctgc agcttatcct gatccatcat
    15481 tccctcaatg acccggcgaa gttcatccac tgtatctccc ggcgtctttc ccgcctcgtc
    15541 gataacaata tccgcatatt tttcatacaa aggcacccgc tcattataga gatctctgag
    15601 cgtaaatccc ggctttaagg caacgccccg atccactaca tttccgatgc gccgttccat
    15661 ttcctcatag ctcatcttta agtagaccac ctcgctgatc tccttcaaat gcttcatggc
    15721 ttcttccccg tagatcacgc tgcctccagg agcgatcacg gaaagccgta catccagccc
    15781 cgcattgatc cggttttcca cctctaaaaa gccatccatt ccttcatctg cgatgatttc
    15841 ctttaaaagg cgtccttcct tttcctggat cacgatatct acatccacaa aggaaaagcc
    15901 cagacgtttg gcaagcagta cgcctatggt acttttaccc gaagacggca taccgatcat
    15961 agtaatattg ggtttcatcc tgcacatcct cctgttcttc agcgtttctt ctttttcacc
    16021 ggtttcttta cagaatatcc gaagcttccc agcttctttc ccagttcaaa atattcaccg
    16081 tgggaatagg ataccagacc ggaggaattt aagttaaatt tcccctttgc atccatgtac
    16141 tgattgtcaa tggttactcc cactacctga gccagaaaca ggtcatggct tcccagttcc
    16201 tttacctcag ttaccttaca ctcaatattt accggacttt ctgcgatccc cggagccttg
    16261 atatatcggg agggctgagg cgtcagacgc atttccttaa acttatccac atcccggccg
    16321 gactttacgc cgcaataatc cgtcgcccgc accagagggc ggtttaccag gttgatcaca
    16381 aactcccctg tatccttaat gatcctatga gaaaaacgtt ccttgcgcac agaaatcgat
    16441 accatggcag gatcagaaca gaccgtgcct gcccaggcta cggtgataat attgggagtt
    16501 tctccctccc tggcgcagct caccatcact gcgggcaccg gataaagcat attgcccggc
    16561 ttccagtatt ctttgctcat aaaaataccg tcctttcctt tactcctgct ctgccagcat
    16621 cagcgccggg atcagctgct tctttctgga taccactccc ggcagacgta ccagaggctc
    16681 tgtttgttct ccggactcat ccggctctgt atgaaaggcc ttttccgcca gctgcagcgc
    16741 tccgtttcct tcgcagatca ggtctgtcga ttcggttaaa atatttgtca gcatgaaaaa
    16801 tacaagatcc agactgtttt cctgtctggc attttccata taatggataa cccttgggcg
    16861 gatgctttcc agctcatctc catttaaaga ggtgatctgt ccaacgccga tagcaatctt
    16921 tcctgaagta aagcgcttaa agtcctgata aaagatctcc tcgtcgctct ttcccttcag
    16981 attgcttccc gcaccaaaca tttccatggc gtatttttct atctcaattc ccgcaatctc
    17041 tgccagcgat ctggcagcca tctcatccat aggcgtacag gtaggagaac ggaacagcag
    17101 ggtatcggac acaatggcgc tgcagagaag tcctgcgatc tttggctcca cctttactcc
    17161 ggcttcctga tacatctgat aaataatggt agccgtacag cctaaaggct gattgcggaa
    17221 aaatacagga gacatggtct gaatggttcc cagcctgtgg tggtcaatga tctccataat
    17281 ctctgcattt tcaattccgg ccactgcctg gttcttttca ttgtgatcca ccagcacaac
    17341 ccgctttccc ttggctccca gcaggtttct tcgggaaatc atacccagat aacggccatc
    17401 cttatccagg atcgggaaat cccggtgacg cttattcgcc atgacgtcac ggatctcatc
    17461 aatataatca tcgctctcaa aggtaatcag gtgctctcgc gtcataaagt agctgatggg
    17521 catactctgg ttaatgagac gggctgccgt gtaggtatca taggttgtgg caataattgt
    17581 acagccccgc tcctgagcaa tctttttgat cgtcatagac actcccgcgc cttcgcagac
    17641 aatgatgcag tccgcgccca tttcaatggc gcaaagctgg gattcatacc ggtttcccag
    17701 aatcaccaga tcatggggct cgatgtaaaa ctccatcaga tcgggatttg ccgccgcgat
    17761 ca
//
LOCUS       DBMDUH010000040        17668 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_1877_length_17668_cov_4.063244, whole genome shotgun sequence.
ACCESSION   DBMDUH010000040 DBMDUH010000000
VERSION     DBMDUH010000040.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 17668)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 17668)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17668
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1877_length_17668_cov_4.063244"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..95
                     /locus_tag="ACZPCG_05195"
     CDS             <1..95
                     /locus_tag="ACZPCG_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HIR1284505.1"
                     /translation="GKKLYDKRADIAKKDQRRELEKEFKVRNLY"
     gene            complement(389..460)
                     /locus_tag="ACZPCG_05200"
     tRNA            complement(389..460)
                     /locus_tag="ACZPCG_05200"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(425..427),aa:Glu,seq:ctc)
     gene            complement(512..583)
                     /locus_tag="ACZPCG_05205"
     tRNA            complement(512..583)
                     /locus_tag="ACZPCG_05205"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(548..550),aa:Glu,seq:ctc)
     gene            complement(1082..2116)
                     /locus_tag="ACZPCG_05210"
     CDS             complement(1082..2116)
                     /locus_tag="ACZPCG_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009249501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HIR1284506.1"
                     /translation="MTNAKKKNTLPSGNCRIQVYDYTDLDGKKHYKSFTAPTKKLAKM
                     AAAEWKAAKEQNRITPDNLTVYDAAGRYIAAKKGTLSPSTIVGYEKIRRNYLKGQIGM
                     TRLPEIDSATVQIWISELSAYLSPKSVRNAYGLLSAVLDMFSPDTQLHIKLPAAKKPA
                     LYCPNDTDIKKLLASIQGTDLELAVLLAAFGPLRRGEISALDSDHIRGNTVTVEFSMV
                     LDENKSWVIKQPKTSGGYRTIEMPSFVIERLRQKKGQVVNLTPNQITQQFGRRLKQLD
                     VPHFRFHDLRHYAASIMHVIGVPDQYILQRGGWASDNVMKAVYRNVIDLETARQNRKI
                     NEHFKQFKIM"
     gene            complement(3174..3722)
                     /locus_tag="ACZPCG_05215"
     CDS             complement(3174..3722)
                     /locus_tag="ACZPCG_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006771061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HIR1284507.1"
                     /translation="MPLPKEQYTSKDYWALPEGSRAELIDGQLYNMAPPSRIHQEITF
                     QIARKLADYIDSKKGNCKVYPAPFAVNLDTDDKNWVEPDISVICDPDKLTDRGCSGAP
                     DFIVEVVSPSSRRMDYLNKTGLYLNSGVREYWIIDPLKKYTTVYLNEQDPAEFAPIIF
                     PFESSIKVGIFSDLSINISRLL"
     gene            complement(3785..3937)
                     /locus_tag="ACZPCG_05220"
     CDS             complement(3785..3937)
                     /locus_tag="ACZPCG_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284508.1"
                     /translation="MFKISNFSLANVDEVEPDPDEEEIMLAYRSGAPGYQAAISQEDL
                     IKELGL"
     gene            complement(3940..4701)
                     /locus_tag="ACZPCG_05225"
     CDS             complement(3940..4701)
                     /locus_tag="ACZPCG_05225"
                     /inference="COORDINATES: protein motif:HMM:NF012920.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HIR1284509.1"
                     /translation="MNTGEKIKAERIKAGYTQKKLADLSGVAEITIRQYESGKREPRN
                     EQLIKIAQTLNVNFYDLIGYAPDDLKQHVKKIKSDIIMDRRKSALLTLFDELNYKGAT
                     KAIEHVEILTRIPEYQKEPASAPESKIIVLPYFRAGVSAGSGIFILGNEAEDEIELPD
                     LPEYEAADFAIDVNGDSMEPDFSHEDIALVQQDAELFPGDIGVFVVNSNAFIKELGEK
                     ELISRNKDYKNIPIHEGDNVVCMGKVIGKVKTDEE"
     gene            4841..5032
                     /locus_tag="ACZPCG_05230"
     CDS             4841..5032
                     /locus_tag="ACZPCG_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016280292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1284510.1"
                     /translation="MKVDRSKLELAMARACMNPADLAIKAGLPRPTLNNAIVGRDVRP
                     VTAGRIAKALGVDVTEIIE"
     gene            5196..5402
                     /locus_tag="ACZPCG_05235"
     CDS             5196..5402
                     /locus_tag="ACZPCG_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284511.1"
                     /translation="MKMPVWAIEFKVDLYALKEFRGWTDEELGKRLGVTARTVGNMRR
                     NPSSVNGALILKVQSMLKEAKEKY"
     gene            5402..5755
                     /locus_tag="ACZPCG_05240"
     CDS             5402..5755
                     /locus_tag="ACZPCG_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284512.1"
                     /translation="MASREELLQSIGPDMRLDKWFFLRIYGYEITWPGFAEVALQRLE
                     IMGCSKARNYYSCIVAEYEHNREEELKEVASWYAEECRKEWDRKVGEQQRAEKLDNMS
                     TEELLTLLRRSVERA"
     gene            5776..7296
                     /locus_tag="ACZPCG_05245"
     CDS             5776..7296
                     /locus_tag="ACZPCG_05245"
                     /inference="COORDINATES: protein motif:HMM:NF024547.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HIR1284513.1"
                     /translation="MGFVDFVNFVKPDLNKISRFPIEALPPVLGDYTRAISESLQVSE
                     DMAAVAVLGVVSLSAMGNFYIEPKTDWVEPLNLYILITARPSERKTPVLKEVSAPVYE
                     YVKEENQRRRPLYDRYLMQKKILENKVSNLISTASKVSKEKKPAASMFDVFDAQQELS
                     NLEEVTLLKLIVDDITPEAMVKVMKENDEKIAMVTAEGGVFGMLAGRYSTQPNMDIFL
                     KAYAGEPYCSDRMGRTGEALDHPLLSLLLMVQPKVLQDALENRDFRERGLMARFLYSL
                     PPSRVGSRVYDSKPIPKELRTAYNQLIKDLLSIGENREWQQENTLIHLSDEAYQISKE
                     FFNEIEQKMAEDYEEIEDWAGKYHGQTMRIAGLIHVVKYRLGAANVVLEGQTMKEAIQ
                     IGRYFLECAMAAFRLSGIAEPQEEKDAKYLMKKIDSFYDGQNGQNRQNLKIRDLHRIC
                     AGHFKTKEDMQPGLDMLVERGFIRIIKKQNKKAGRPPEFIEVNPEYWKSKEMIDFS"
     gene            7638..7859
                     /locus_tag="ACZPCG_05250"
     CDS             7638..7859
                     /locus_tag="ACZPCG_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284514.1"
                     /translation="MNKDSYMVKRHKHRCGRLGLIANTLFASAGAGTVAFLVTAMVFQ
                     TDGLLAFLMAYVAACMAAVINPLWLSGRG"
     gene            7914..8105
                     /locus_tag="ACZPCG_05255"
     CDS             7914..8105
                     /locus_tag="ACZPCG_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284515.1"
                     /translation="MKLKDAVFCGFTKKELEKVHKAKGLGEREISLTDSQDIADIVEA
                     DVMTMITPGGQITFIKAGE"
     gene            8108..8287
                     /locus_tag="ACZPCG_05260"
     CDS             8108..8287
                     /locus_tag="ACZPCG_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284516.1"
                     /translation="MAYGYMRPDPVEQFRGAVGHYGDLILAAVQMGYTESEAIELIKV
                     YALFDIANKLDGISF"
     gene            8408..8635
                     /locus_tag="ACZPCG_05265"
     CDS             8408..8635
                     /locus_tag="ACZPCG_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284517.1"
                     /translation="MDIQKEIKISEETPVVFLPDTEMRELYRAMVKRWKLHAIDAFGA
                     SLFYSLGRVHGIRQERARRKAAQLKTAPGKK"
     gene            8802..8957
                     /locus_tag="ACZPCG_05270"
     CDS             8802..8957
                     /locus_tag="ACZPCG_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284518.1"
                     /translation="MIKLKVSYEHPEELRRLLKKLGPDVKSWKASQNREGKFLKAYVV
                     MKDTREN"
     gene            8970..9185
                     /locus_tag="ACZPCG_05275"
     CDS             8970..9185
                     /locus_tag="ACZPCG_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284519.1"
                     /translation="MRNSTAIRGMRTREQYKVMEFINRNFEPGALEIELVDGETIRGI
                     DRNGRSMIFRWDQSTREVEALDDLVTR"
     gene            complement(9524..10339)
                     /locus_tag="ACZPCG_05280"
     CDS             complement(9524..10339)
                     /locus_tag="ACZPCG_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016285111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR1284520.1"
                     /translation="MIKEIQSFWAESEKKRIKTVAEQFHLSLPCAKKYIYMSSKELEE
                     LDTPKNYKKRESAMNEWLNVIFKMMLDGHTNETIYFYIKEHPDFNESEKKLQKYIYLI
                     GKNNFPDRIPFNANYLMEKVLPPDVICFKRTEILKYLLTCNPKKQKDLELGKYIDVIK
                     EAYPVAAYVETVFKDFHRIIMGNSPDEIDVFIEQYEESVISTFCKGLKKDIAPVKNAI
                     SHRESSGFVEGNNNKFKLLKRIVYGRSGLVNLSKKCKLAFMLKDDAFTLSSLI"
     gene            complement(10336..11316)
                     /locus_tag="ACZPCG_05285"
     CDS             complement(10336..11316)
                     /locus_tag="ACZPCG_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016288635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HIR1284521.1"
                     /translation="MKYQEITDYSFLLGENHYIYKHTEDDDTLHLFVKSRPHEAKCPY
                     CGMVSRSLHATYERVLQDTPIHCKQTYLHANVYKYDCENPDCDHTVFMEDLPFAKASQ
                     VRTDALNSLILGVSMFLSNEGASKVLSLLGVKVSNDTIQRLYNRIDFIDNPDVEEIGI
                     DDVAIRKGQTYATAVYDLKDHHLIALLKGRDAETLKEWLKNHRKVRLVARDRASAYAA
                     AINAVLPECVQVADRFHLLQNLLEHLKDIFKADMPATIYIKNEKILEQEPEKILKEKA
                     PDYTYLSTLNYDCTPPKNPDGSERSFDNKKHDLASSKYKELQIQRKKNKN"
     gene            complement(11543..12436)
                     /locus_tag="ACZPCG_05290"
     CDS             complement(11543..12436)
                     /locus_tag="ACZPCG_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1284522.1"
                     /translation="MICPEKIKYIFKKRYKKIKILKIAYHHNICYNELLKENPIMNSK
                     SVKPLYQQIEASIRSDIQTHIYKTGDKLPTETELSAKYNVSKITIRKAMEKLSHNGLI
                     QKVQGKGTFVCPQKDKIALSETRGFNDSLSSHGHSSRHKVLEIKKIQSDDRISEALSL
                     REGSPVIYVERLIWADNKPIGLDTIYVSETTFPDFIQKAASEKPLYHILREDYGLEVA
                     EATLEINGILADSSKASLLKCLVGDPLFHVEKTAYNSDGIPIHFSSTYVRCDSVTYVV
                     RTNQYLHMEEKRLASCFHKRK"
     gene            12494..13471
                     /locus_tag="ACZPCG_05295"
     CDS             12494..13471
                     /locus_tag="ACZPCG_05295"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIR1284523.1"
                     /translation="MRQEHLGQIVQAVSAVEQMGTVNAVYFVACGGSQAVLMAGQYIF
                     DKETTVPSHVYTANEFMYDTPKSLNEQSLVISCSHSGNTPETVAATKLAREKGAMTIA
                     LSNAEGSPLWNAAQCPVHYDWGKEVSDSDKNKGILYGLLFHLLKVLAPSEKWDVCLKE
                     LDKLSELSGKAKELYQEKAERWAKENKREKLIYTIGSGINYGEVYSTAMCWFMEMQWI
                     HSGCIHSGEYFHGPFEVTDYDVPFLLVKSIGNTRFLDQRVEDFSKKYSDKMLILDQED
                     LPLAGVADEAKPYIAAILSGVVIRQFVEAIAFERGHSLDVRRYMWHMEY"
     gene            13502..14173
                     /locus_tag="ACZPCG_05300"
     CDS             13502..14173
                     /locus_tag="ACZPCG_05300"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1284524.1"
                     /translation="MLPQLVIFDVDGLLLDTEQVWKQAWRKTADSFGLLNVRDEEFHR
                     LIGKTGQASMDEAREILNGACRAEDFMERMRAFGSKMLEEDLRLKPGARELLEKLQEK
                     EISCAVATATSRELTEKRLGRMGILSFFSYVCCGDEVRRGKPFPDIYLNVLEQMKCPA
                     DRAVVLEDSPVGVEAAFRAGIPCVMVPDMILPGEAEKERAKWILPSLFEAGRRIFQED
                     SAGTT"
     gene            14198..14629
                     /locus_tag="ACZPCG_05305"
     CDS             14198..14629
                     /locus_tag="ACZPCG_05305"
                     /inference="COORDINATES: protein motif:HMM:NF015567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIR1284525.1"
                     /translation="MEKEDFGILMVTHGGLAKGYLSALKLVLDIDETDLDTVCFEEGE
                     STEEFEQCLRTVIEEQYRDRNLVILLDIPGGTPANTALRFLSDSRRLIAGANLTVAVE
                     VMMEKKNHTPWDKLNLDSIAEDGKNSIVYYNRLLKEEQDYD"
     gene            14622..15086
                     /locus_tag="ACZPCG_05310"
     CDS             14622..15086
                     /locus_tag="ACZPCG_05310"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015769.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HIR1284526.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTRQTAANHIIVADDLVPNDPIGKM
                     ALSLAKPSGCSLDIIPFSELKQTVESNADKNIMIIVRGPKEALRLVHELPQVREINYG
                     GVAKKQDSKPYGKAVYLNKEELKDTQEILSEGISIYIQQVPTSQIEHADFSK"
     gene            15114..15857
                     /locus_tag="ACZPCG_05315"
     CDS             15114..15857
                     /locus_tag="ACZPCG_05315"
                     /inference="COORDINATES: protein motif:HMM:NF015566.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HIR1284527.1"
                     /translation="MLISSILLGLVGIIAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     RAGVTLGASLELISMGFVAIGAAGPPNMQLGSIIATSFAILSGASTEAALTIAIPVAV
                     IGEFMSVIMRIIIAQFSHVADRAIEEENYKKAQNIHIWWSFGLNCVVYFVPIFLTIYF
                     GTDLVDTMVNAIPEVITNGLTVAGNLLSALGFSMLLSTMLSKKLFPYFLFGFLVVAYS
                     GLDLIGLTLFACILAFIMDKVVYGRRESA"
     gene            15860..16696
                     /locus_tag="ACZPCG_05320"
     CDS             15860..16696
                     /locus_tag="ACZPCG_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HIR1284528.1"
                     /translation="MEEMNQTVAEGAVTKKDLRRLFLRSWTIMCSWNYERQMHMGFMF
                     GMAPILDKLYKNKPEKCREAYQRHMEFFNCTPQVTGFIMGLAASMEEQNAKCEDGSFQ
                     PESISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAQQGNVLGPILAVLLFAIPS
                     IIMGYYGTVLGYQSGNKYLTKLYQEGIMERVMQCASIVGLAVVGGMVASMVSITTPLA
                     FHAGGTELVLQDMLDTMFPKLLPLLATLGIYRLIQKKVNTNLLLLGIVICGIVLGALG
                     IL"
     gene            16990..>17668
                     /locus_tag="ACZPCG_05325"
     CDS             16990..>17668
                     /locus_tag="ACZPCG_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004842833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR1284529.1"
                     /translation="MSIVNTLSLESNRQIKINFDGGDLSSDAGLLLIKEFVSKPGIDN
                     LLGKVFKTNDSALFRYHTDQKNLLQMIYMIIAGYFEDDTSDELTNDPVFKSVLEKDTL
                     ASQPTVSRFFNRMDEDTLNQLLMIGRILRKRVYRIQMPQAVILDLDSTLLDAYGKQEG
                     RAFNFHYQSNGYHPLVCYNGMTGDLVKIQLRDETEYSSTGVVDFLQPILDEYLNDYPA
                     IRILLRGD"
BASE COUNT         5037 a         3321 c         4293 g         5017 t
ORIGIN      
        1 gaggaaagaa gctttacgat aagagagcgg atattgccaa aaaggatcag cgcagagaat
       61 tggaaaaaga attcaaggtg aggaatctgt attaatctga aaagagttta tgcaaatgaa
      121 aacgtacagc tgcaggaagc ttgtgctccg aaaggctgta cgtttttgtt tacagtttat
      181 taaaatgttt tctgtagacc tggaaggaca ggattactgt aaatatatca gctgtaaact
      241 gagaccaaac aaggccatac atgccaaaga cggcatttaa cagaaacagc atgggaatat
      301 tgaaggcgct gacctttttc acgtcctctt atctgtagat ggtaaaaaca caaaaaaaca
      361 agcagaaaat tacactgctt gtttcaaatg acctgaccgg gaatcgaacc cgggtttacg
      421 ccgtgagagg gcgtcgtctt gaccgcttga ccatcaggcc ttattaaatt gacacataaa
      481 tacgaaagta taattataaa acactttcaa atgacctgac cgggaatcga acccgggttt
      541 acgccgtgag agggcgtcgt cttgaccgct tgaccatcag gccttattaa tactgtatcc
      601 tcatcgctga gaactcgtat agtctaccat gagtttttcg atttgtccag tgttttttgt
      661 aaaaaaaata aagatgttta attgataaaa ataaagagaa tattcagaaa attcattttg
      721 ttaaaaaagg aggaggccgg ctgaattctt ttcagccggc cgagtattat gaaaaaaagt
      781 ttaatagcat taactcttta caatgttcag tatagaaagc aaacatgaag agagtttgat
      841 caatctgtaa cagaattatg aacagagtgt aagaagtttg tgaatggaaa ttgtgagagg
      901 tctgaaatgc aggataaaac cttggcagat ctggaaaacg ggctacgaaa aaaccgcata
      961 aacactggct ttcccagcat ttacgcggtt tttaaaaagt ggagctgagg ggaatcgaac
     1021 ccctaaaaaa ccctagtatt tatcagcatt tccgggccgc gtgtgatatt tcgtgtgata
     1081 cttacatgat tttgaactgt ttaaaatgct cgttgatctt cctgttttgc cgggctgtct
     1141 ccagatcaat cacattccgg tatacggctt tcattacgtt atcagaagcc cagccgcccc
     1201 gctgtagtat gtactgatcc ggtacgccga tcacgtgcat gatcgaggcg gcataatgtc
     1261 tgaggtcgtg aaagcggaag tgaggtacat caagctgttt aagcctgcgc ccaaactgct
     1321 gcgtgatctg gttcggggta aggtttacca cctggccttt tttctgcctc aggcgttcaa
     1381 tgacaaagga cggcatttca atggtacggt atccgccgga ggttttgggc tgcttgataa
     1441 cccagctttt gttttcgtcc agaaccatag aaaactctac tgtcacggta ttcccccgga
     1501 tgtgatctga atcgagagca gagatctcac cccgtctaag cggcccaaag gctgccagca
     1561 gtacagcaag ctccagatcg gtcccttgga tactggcaag aagctttttt atatccgtat
     1621 catttggaca gtacagggcc ggctttttag cggctggcag tttgatgtgc agctgggtat
     1681 ctggggaaaa catatccaga acagcagaaa gaaggccgta ggcgttcctt acgctcttgg
     1741 ggcttaaata ggcagataat tcacttatcc atatttgcac ggttgcgctg tctatctccg
     1801 gaagccgtgt catgcctatc tggcctttta aatagttccg gcgtatcttt tcatacccga
     1861 ctatggttga aggggaaagc gtgccctttt tggctgcgat atagcgccct gcagcgtcat
     1921 ataccgttag attgtccggt gtgatgcggt tctgctcctt ggctgccttc cattcagcag
     1981 cggccatttt cgccagcttc ttagtgggag cggtaaagct cttgtaatgt ttcttcccgt
     2041 ccagatctgt ataatcgtat acctgtatcc ggcagtttcc tgagggaaga gtgttctttt
     2101 tctttgcgtt tgtcatgtgc gttgctcctt tctgatcggg agagccgcac aggacggcaa
     2161 agacagcgta atagttctac aaaagggcag aggccccctg tatacgttgt aatgtgtttc
     2221 aatgcggttc ctcctgatcc tctgccgggc tggggcgcat ggatggaagg aagagcgtat
     2281 ttgacaaata ggtgtgattg gaatataata tacttaacaa gacagccagt aagggaggtc
     2341 aaggctcccc gccctggtga ttataagaag ctaagaagta gccgcctatt ctttaccaga
     2401 gagcagggcg gctatttctt atgtgtgtat gtcaggatcg caacgatcag taatgctacg
     2461 gatacaatta actgcaattc ttcatatgta ctcataagta tcccctccct tccaagactc
     2521 agggcgggaa ccgcagccgc tctactggct gcccgggtaa gtatattata ttgttaatgt
     2581 gtcgggtgtc cttatctcgg acacccccag atgttaccat tagtcacacc tgaaagtcta
     2641 aggtctggaa taccgacttt aaagtatgcg gtaaaatacc gtataccctt tgggcggttg
     2701 gttaaactga ctttaaaggc taagaggtgc atcgtatgca cttctggata cggcgtttta
     2761 ccgtagctgt ggttttactc atttttaagt aaaacagagc tttcccggta agttgggaaa
     2821 agctggtttt gtcccgtccc tgatttaatg gctgcatgtt aaggtttcgc acttggacgc
     2881 aaaattgcgc tcgtggctga aaattcagct gccacattta ggcctctgcc cctcagaatc
     2941 aacccgctca aaaatgagca ggttcgtttt ctaaagggtg tcccacaaat gtcctaccct
     3001 tgaaggagag cagggctata acgtttcatt ctggtagcct caagtaccaa aacggtaggg
     3061 gcacatgaca acatcagtaa aaaagggtgt cgcgtttcac gacaactcaa taacattttg
     3121 ttattcagtg gggtaacgtt ttgttactcc ttggtgttgc gacgtcgcaa cgatcacagc
     3181 agccgactga tattgataga caggtcagag aagattccaa ccttgatact gctttcaaac
     3241 gggaagataa tgggggcaaa ctctgccgga tcctgctcat tcaggtacac agtggtatat
     3301 ttttttaaag ggtctataat ccagtattcc cgaacgcctg agttgagata aagtccggtt
     3361 ttattcaaat aatccatgcg gcggctgctg ggagatacca cctcaacaat aaaatcagga
     3421 gcgccggagc atccccggtc tgtcagcttg tcaggatcac agattacaga aatatccggt
     3481 tccacccaat ttttatcatc agtatccaga ttaactgcaa atggagcagg atatactttg
     3541 cagttacctt tttttgaatc aatataatcc gccagcttac gggctatctg aaatgtgatt
     3601 tcctgatgga tccggctggg cggggccatg ttgtaaagct gtccatcaat cagctcagcc
     3661 cgtgaacctt ccggcaaggc ccagtaatct tttgatgtgt actgttcttt gggtaatggc
     3721 atgaagtacc gtcctttctt tgttgggggg ggcaaataat agcttctagg gggcagaggc
     3781 aggcttacaa ccctaactct tttattagat cttcttgaga aatagcagcc tgataaccag
     3841 gggcaccgga tctataagca agcattattt cttcctcgtc tggatcgggt tcgacttcat
     3901 caacgtttgc taaagaaaaa tttgagattt taaacattgc tattcctcat ccgtctttac
     3961 tttcccaatt acttttccca tacagacaac attatcccct tcgtgtatgg gaatattttt
     4021 ataatcttta tttctggaaa taagttcttt ttctcccaat tctttgataa aagcgttgct
     4081 gttcactaca aaaaccccga tatctccggg gaacaactcc gcatcctgtt gcaccagtgc
     4141 aatatcttca tgtgaaaaat caggctccat gctgtccccg tttacatcaa tggcaaagtc
     4201 ggcagcctcg tactctggca gatccggcag ctctatttca tcctctgcct cattgccaag
     4261 gataaagata cctgatccgg cagacactcc ggcccggaag taggggagaa caatgatttt
     4321 gctttcaggg gcagaggcag gctctttttg gtattcgggg attctggtta gtatttccac
     4381 atgttctata gctttagtgg ctcctttgta atttagttca tcaaataatg taaggagggc
     4441 actttttctg cgatccataa taatatctga tttgattttt tttacatgct gtttcaaatc
     4501 atcgggggca tatcctatta aatcataaaa attgacattt aatgtttgag caatttttat
     4561 taactgctca tttctcggtt cgcgttttcc tgattcatat tgccttattg taatttctgc
     4621 tacaccactt aaatcagcga gttttttttg ggtgtatccg gccttaattc tttctgcttt
     4681 tattttttca cctgtattca aattatcacc tctgaaacca taataccata gttttaaata
     4741 aaaaaacaga ttcaaacgaa taaaaattat tattgacaga ttcaaatgaa tgtgatattt
     4801 ttgaaaaaga ttcaaatgaa tctattaagg aggtgagaaa atgaaagtag acagaagcaa
     4861 gttagagctt gcaatggcaa gagcctgcat gaatcctgcg gatttagcaa taaaagcagg
     4921 tctcccacgg cctacgctta ataatgcgat agttggccgt gatgtacgac ccgtaacagc
     4981 cggccgcatt gccaaggctc ttggcgtgga tgtaactgaa attattgaat aagctccggg
     5041 ctggggcgca tggacgaggg gaagaacctc tcagttgtac cggcgcaact ctgcgctggt
     5101 tcccgatcgg gtaagggctg gccgttctgg ctgggctgat agatgaagaa tttaaaatat
     5161 aactcaggca gaacacgccg gaaaggagtg attacatgaa aatgccagta tgggcgatag
     5221 agttcaaggt ggatctgtac gctttgaaag agttcagagg ttggacagat gaagaattgg
     5281 ggaagcgttt gggggttacg gcccggacgg tcggcaatat gcgccggaat cccagcagcg
     5341 taaacggtgc tttaattctt aaagtacagt caatgctcaa agaagcaaag gaaaaatact
     5401 gatggcaagc cgtgaagaac tgctgcagag cattgggccg gatatgaggc ttgataaatg
     5461 gtttttcctg cggatttatg gctatgaaat tacatggccg ggatttgcag aggtagcgtt
     5521 gcagcggttg gaaatcatgg ggtgcagtaa ggccaggaac tactacagct gcatagtggc
     5581 tgagtacgaa cacaaccgag aagaagaatt aaaagaggtt gcatcctggt atgcagaaga
     5641 atgccggaaa gaatgggaca gaaaggtagg tgagcagcaa cgagcggaga aattggacaa
     5701 catgagcacg gaggaactgc tgacattatt gcggcgctca gtcgaaaggg cgtagaggtt
     5761 actttttcgg attctgtggg ttttgtggat tttgtcaatt ttgtcaaacc agatttaaat
     5821 aaaatttcac gtttcccaat agaggccctt ccaccagtgc tgggagatta tacccgtgct
     5881 atatcggaaa gcctgcaagt atcggaagat atggccgctg tagcagtttt gggagtggta
     5941 tctctatctg ctatggggaa tttctacata gaacctaaaa cagattgggt ggagccatta
     6001 aacctctata tattgattac agccagacca tcagagagaa agaccccagt gctgaaagag
     6061 gtatctgctc cggtttatga atatgtcaaa gaggagaatc agagaaggcg tccattatat
     6121 gatagatacc tgatgcagaa aaaaattctt gaaaataaag ttagtaatct tatttcaaca
     6181 gcttctaagg tatcaaagga gaaaaagcct gctgcttcta tgtttgacgt atttgatgca
     6241 cagcaggagc tttcaaatct ggaagaagtt acactcttga aactgatagt agacgatatt
     6301 acgccggaag ctatggtaaa ggttatgaaa gagaatgatg agaaaatagc catggttacg
     6361 gctgagggag gtgtttttgg tatgttggca ggaagataca gcacccagcc caatatggat
     6421 atatttctaa aggcctatgc gggagagcct tattgctctg atagaatggg aaggactggg
     6481 gaagcgttgg atcatcctct tttatcactg cttttaatgg ttcagcccaa agtattgcag
     6541 gatgctttag agaataggga ctttagagaa cggggactta tggcaaggtt tttatattct
     6601 cttcctccgt caagggtagg gagcagggta tatgattcaa agcccatccc aaaggagtta
     6661 agaaccgctt acaatcagct gataaaggac ttgctttcca ttggtgaaaa tagggaatgg
     6721 cagcaggaaa acacactgat ccacctcagt gatgaagcct atcaaatcag caaagagttt
     6781 ttcaatgaga ttgagcagaa aatggcagag gattatgagg agatagagga ctgggccgga
     6841 aaatatcacg ggcagactat gagaatagct ggacttatcc atgtggtaaa gtacaggctt
     6901 ggagctgcca acgtggtact tgaaggccag accatgaaag aagcaataca gatcgggcgg
     6961 tatttcttag agtgtgctat ggcagcgttc cgattgtcag gtattgcaga gccgcaggaa
     7021 gagaaggacg caaagtattt gatgaagaaa attgattctt tctatgatgg acaaaatgga
     7081 caaaatcgac aaaaccttaa aataagggac ttgcatcgga tttgtgccgg tcattttaaa
     7141 acaaaagagg atatgcagcc ggggttggat atgctggttg aaagaggttt tatccggatc
     7201 ataaagaagc agaacaaaaa agccgggagg cctccagagt tcatagaggt aaacccggaa
     7261 tactggaaga gtaaagagat gatagacttt tcatagagag taaaggagaa aagaggacaa
     7321 gtttccattc cgttcagtgt gggtacttcc tggacatata gaggacgaac cggaagcaga
     7381 acagcaggag cttgatttat agcatggtta aaccctatag gcaaacttct aaacttggcc
     7441 caacgggaga aaatccctta aaatcaatgt ttttatcgtt ggttcaattt ggccttacga
     7501 ggtcagcggc gcgcgttagt ccgttggttc aatttggcct tacgggaaaa actcagtaaa
     7561 attaagagtt ttacgccgtt gtgtcatata agtatggtta cctatagagc gcagagggaa
     7621 aggagggaaa aaggactatg aataaagatt cgtacatggt aaagcggcat aaacaccgct
     7681 gcggaaggct gggactgatt gcaaatactc tgtttgcctc tgccggggcc ggaacagtgg
     7741 ctttcttggt aacagcaatg gtatttcaga ctgatggcct gctagcgttc ctgatggctt
     7801 acgttgccgc ctgtatggct gcggttatca atccgctgtg gctgtcgggc agaggataaa
     7861 acagggcttg aagcccgaac gaaccctgag aaaacaaaag gaggacaagc acaatgaaat
     7921 tgaaagatgc agtattttgt ggatttacca agaaggaatt ggagaaggtt cacaaggcca
     7981 agggattagg agagagggaa atttccctca ccgattccca ggacatagcg gacattgtgg
     8041 aagctgatgt aatgacaatg attacaccag gcggacagat tacatttata aaggcaggtg
     8101 aataattatg gcatatggat acatgaggcc ggatccggta gagcagttta gaggcgcagt
     8161 aggacattat ggcgatctga ttcttgcggc ggtacagatg ggatacacag agtcagaggc
     8221 aatcgagctg attaaggtgt atgcactctt tgacattgct aataagctgg acggaatcag
     8281 cttttaagaa caaagaaaaa gccctcaggg agcgccaact ccctgggggc agacatagcc
     8341 ggagccatgc acatatacaa cgcatacata gtgtagcatg gcccggcgga aagggaaaga
     8401 gaaaattatg gatattcaaa aagaaatcaa aataagcgag gaaacgccag tggtattcct
     8461 tcctgacaca gaaatgaggg agctttatcg tgctatggtg aagcgttgga aactgcacgc
     8521 aatagacgct tttggcgcct ctttgtttta cagtctgggc cgtgtgcatg gaatccggca
     8581 ggagcgcgcc agaagaaagg cggcccagct caaaactgcg cccggtaaga aatagaacgg
     8641 cttttacata gattttcaaa ggcaatatgg tttgaaccgc tggccgttgc gagagtgcgg
     8701 cagaaattaa aagggggcgc tggatcagtt gccccttttt gggtgaaagg tgtttctgat
     8761 ctggtaacac ctcatttacc ggatcagaga aaggagaaac catgataaaa ttgaaagtat
     8821 cgtatgagca cccggaagag ctcagacggc tcctaaagaa gctggggccg gacgtgaaga
     8881 gctggaaggc ctcacagaac cgggaaggga agttcctgaa agcttatgtt gtgatgaaag
     8941 ataccaggga aaactagagg ggtgatggaa tgaggaacag tacagccatt agaggcatga
     9001 gaaccaggga gcagtacaaa gtgatggagt ttatcaaccg gaactttgag ccgggagcat
     9061 tggagattga actggtggac ggtgaaacca tccgggggat cgatcggaat gggaggagca
     9121 tgatatttcg ctgggatcag agcaccaggg aagtagaggc gttggatgat ttggtaacac
     9181 gataatgcgc gggaggcagc caagaattta atgacacgat aacgcacggg agctgctgcc
     9241 tgttctgtgc gacgtcgcaa cactatagcg agcgttttca aaaccgataa aaacgacgaa
     9301 aacaaacacg aatacaaaca cgaatacaaa caaatacccc taaaaaagtg cttaaaacct
     9361 agtaaaatca atgattccaa gattgcaacg ttccgaacgt ttattagaag gttgcaataa
     9421 aaaaacaaac acaaatagag acaggaagcg ggtcgggttc tttctgaact ttgggtactg
     9481 tgaagtatta tttatgctgg catcgaaaag atgccagcat tttctatatc aatgacgaga
     9541 gtgtgaacgc atcgtctttg agcataaatg ccagtttgca cttctttgag agattgacta
     9601 atccacttct tccgtataca atacgtttga ggagtttgaa tttgttgttg tttccttcaa
     9661 caaatccaga actttcacga tgggatatcg cgttcttgac cggggcgata tcctttttta
     9721 atcctttaca gaaagttgat atgacacttt cttcatactg ttcgataaaa acatctatct
     9781 cgtctggaga attccccatg ataattctat gaaaatcttt gaacacagtt tcaacataag
     9841 cagcaaccgg ataagcttcc tttattacat caatatattt ccctaattcc agatctttct
     9901 gtttcttagg attacatgtc agaagatatt tgagaatttc tgttcgtttg aaacagataa
     9961 catctggcgg cagaactttt tccataagat agttcgcatt aaatggtatc cggtccggaa
    10021 agttattttt tcctatcaga tatatgtatt tttgcagttt cttttcagat tcattaaaat
    10081 cgggatgctc ttttatgtaa aaataaatcg tttcatttgt atgtccatcc agcatcatct
    10141 taaatatcac attcagccat tcattcatgg cagattctct ttttttatag tttttagggg
    10201 tatccagttc ctccaattct tttgatgaca tatagatata ctttttggcg cagggcagtg
    10261 ataaatgaaa ctgttctgca accgttttta tccttttctt ttccgattcc gcccaaaaac
    10321 tctgtatttc tttgatcaat tcttgttttt tttacgctgt atctgcagtt ctttatattt
    10381 agatgatgca agatcatgtt ttttgttatc aaaactccgt tcggaaccat ctggattttt
    10441 gggcggtgta caatcataat ttaatgtaga aagatatgtg taatcaggag ccttttcctt
    10501 caatattttt tcaggctctt gttccagtat tttttcattt tttatataaa tagtagccgg
    10561 catatctgct ttaaagatat ctttcagatg ttccagaaga ttttggagca gatgaaaacg
    10621 gtcggctact tgaacgcatt ctgggagaac agcattgata gcggcggcat aagcactggc
    10681 acggtcacgt gctaccaatc gaactttccg gtgatttttc agccattcct ttaatgtctc
    10741 tgcatctctt cctttaagaa gcgctatcag atgatgatct ttcaaatcgt aaacagcggt
    10801 tgcataagtc tgtcctttac gaatggccac atcatcaatc cctatttctt ctacatctgg
    10861 attgtcaata aaatcgattc tgttatacaa acgctggatg gtatcgttgc tgaccttaac
    10921 tcctaaaagt gacaatacct tgcttgctcc ttcattactg aggaacatag atacaccaag
    10981 aatcaacgaa ttcaatgcat ctgtacgaac ctgtgaagcc ttagcaaatg gaaggtcttc
    11041 cataaaaacc gtgtgatcac aatcgggatt ttcacagtcg tatttataaa catttgcatg
    11101 aagatatgtt tgtttacaat ggattggtgt atcctgcaag actctttcat aagttgcgtg
    11161 cagggaacgg cttaccatgc cacagtaagg gcatttggct tcgtgtggtc tggatttgac
    11221 aaagagatgt aatgtgtcat catcttctgt atgtttatag atataatggt tttctcccaa
    11281 taagaaagag taatctgtaa tttcttgata tttcatctga ttctatacct cttgatgttt
    11341 atattcatca ctattatacc agaaataccc aaaatagaga agttattgca tgaagaaaaa
    11401 gcaggtattt ttcaatacct gctttaaaca taatacccaa agttcagaaa gaacctattt
    11461 taagtcacag tccttctttt tgttgtattt tctgcagtct tatatcaggc agaataaggg
    11521 ggattttgat aaaaactttt tattattttc ttttatgaaa acaggaagcc agcctttttt
    11581 cttccatatg cagatactgg tttgtacgga caacataggt aactgagtca cagcgtacat
    11641 atgtggagga aaaatggata ggtatcccat cggaattata ggcagttttt tctacatgga
    11701 acagagggtc gcctaccaga cattttagga gggatgcctt agaactgtcg gctaaaattc
    11761 cattaatttc cagagtggct tccgctacct ccagaccgta atcttctctt aagatatgat
    11821 aaagaggttt ttcagaggcg gctttctgta taaagtcagg aaaggtggtt tctgaaacat
    11881 agatggtatc aaggccaatg ggtttattgt ctgcccagat gagccgttct acataaataa
    11941 caggactgcc ttcacgcagg ctgagagctt ctgaaatacg gtcatcggat tggatttttt
    12001 tgatttccag tactttatga cgggaggagt ggccatgaga ggaaagggaa tcgttaaatc
    12061 ctcttgtctc ggagagggca attttgtcct tttgtgggca cacaaaggta ccttttccct
    12121 gaaccttctg tattaatcca ttatgggaaa gtttttccat agctttacgt atggtgattt
    12181 tgcttacatt atattttgcg cttagttcgg tttctgtggg aagcttgtca ccggttttat
    12241 aaatgtgtgt ttggatatcg ctgcgaatgg atgcttcaat ctgttgatac aatggtttta
    12301 cactttttga attcataata ggattctcct tcaacaattc attatagcat atattatgat
    12361 gatatgcaat ctttaaaatt tttatctttt tgtatctttt tttgaaaata tacttgattt
    12421 tttcgggaca aatcactata ataagatata taaagataca taatgatata taattaatta
    12481 ggaggagacg gatatgagac aagaacatct tggacagatt gtacaggcgg ttagtgctgt
    12541 ggagcagatg ggaacagtga atgctgttta ttttgtggcc tgtggaggct cccaggcagt
    12601 tttgatggca gggcagtata tttttgataa ggaaacaacg gttcccagcc atgtgtatac
    12661 agcgaatgaa tttatgtatg atacacccaa gtctttaaat gaacagtctc tggtaatcag
    12721 ctgttcccat tccggtaata ctccggaaac ggttgccgct acaaagcttg ccagagaaaa
    12781 aggagctatg acgattgctt tatcaaatgc agaaggatcg cctttatgga acgccgctca
    12841 gtgtcctgtt cactatgatt ggggcaagga agtgagcgac agcgacaaga ataaaggtat
    12901 cctttatggt cttcttttcc atctgctgaa ggttttggct cccagtgaaa aatgggatgt
    12961 atgcctgaag gagcttgata agctgtctga gttgtcagga aaggcaaagg agctttatca
    13021 ggaaaaggcg gagcgctggg caaaggaaaa caagcgggag aagcttatct ataccattgg
    13081 aagcggaatt aactatggag aagtgtattc tactgcgatg tgctggttta tggaaatgca
    13141 gtggatccac tccggctgca tccattccgg tgagtatttc catggaccat ttgaggtgac
    13201 agattatgat gttccgtttt tattagtaaa aagtattggc aacacacgat tcctggatca
    13261 gagagtagag gatttcagta agaaatattc agacaagatg cttattctgg atcaggagga
    13321 tcttccgctg gcaggagtgg cagatgaggc taagccttat attgcagcca ttctgtccgg
    13381 agtagttatt cgtcagtttg tagaagcaat tgcttttgag cgtggacatt ctttggatgt
    13441 gcgtcggtat atgtggcaca tggagtatta atcaaccgga gaaagaagga ggggattaaa
    13501 catgctgccg caattggtta tttttgatgt agacgggctt cttttggaca cggagcaggt
    13561 gtggaaacag gcatggcgca aaacagccga ttcgtttggg cttctgaatg tacgtgacga
    13621 ggaatttcat cggttaattg gaaaaaccgg acaggcttca atggatgagg cacgtgaaat
    13681 tttgaacggg gcttgccgcg cagaagattt tatggagcgt atgagggcct ttggctccaa
    13741 aatgcttgag gaagatctgc ggttaaagcc gggggcacgg gagctgcttg agaagctgca
    13801 ggaaaaagaa atttcctgtg cggttgctac ggcaacctct cgtgaactga ctgaaaaacg
    13861 tctggggcga atgggaatcc tttccttctt ttcttatgtt tgctgtgggg atgaggtgag
    13921 aaggggaaaa ccattccctg atatatacct gaacgttttg gaacagatga aatgtcctgc
    13981 cgacagagca gtagttctgg aggattctcc cgtaggagta gaggctgctt ttcgggcagg
    14041 gattccctgt gtaatggttc cagatatgat tttgccggga gaagctgaaa aggagcgggc
    14101 aaagtggatt cttccgtcct tatttgaggc gggccgccgg atttttcagg aagacagtgc
    14161 aggtacgaca taaacaaagg cttaagaggt gaaacatatg gaaaaagaag atttcgggat
    14221 tttgatggtt acccacggcg gactggcaaa agggtatttg agcgctttaa agctggtttt
    14281 ggatattgat gagacagatt tagacacagt atgttttgaa gaaggagaaa gcacagagga
    14341 gtttgaacag tgtttaagga ctgtgattga agaacagtat agagacagaa atctggtaat
    14401 cctcttggat atacccggcg gaacgccggc aaatacggct ttgcggtttc tgtcggattc
    14461 aagaaggctg attgcaggag ctaatcttac tgtggcagtg gaagtaatga tggaaaagaa
    14521 aaatcatact ccctgggata aactgaatct ggattccatt gcagaggatg gaaaaaacag
    14581 cattgtttat tataacaggc ttttgaagga ggaacaggat tatgattaaa ttagtgcggg
    14641 tggatcacag gctgctgcac ggacaggtta ttttttcctg gacccggcag acggcagcga
    14701 atcatattat tgtggcggac gatctggttc ctaatgaccc catcggaaag atggcattgt
    14761 ctcttgcaaa gccgtcaggc tgttcattag atattatccc tttcagtgaa ttgaaacaga
    14821 cagtggagtc caacgcggac aagaatatta tgattattgt aagaggccct aaagaagcgt
    14881 tgaggcttgt ccatgagctg cctcaggtca gagagattaa ttacggcggc gttgcaaaaa
    14941 aacaggattc taaaccatat ggaaaagcag tgtacctaaa taaggaagaa cttaaggata
    15001 cacaggagat actgtcagag gggatcagca tttatataca gcaggttccc acctctcaga
    15061 ttgaacatgc tgatttttca aaatagcaga ttgagcagag agggggtaat attatgttga
    15121 tttcatcaat tttgctggga ctggtgggaa ttatcgcagt tattgactca cggttaattg
    15181 gccgtcagaa tattggcagg ccgttgattt taagtacgct ggcaggtctg gttctgggag
    15241 atttaagagc tggagttacg ctgggagcca gtctggagct gatttccatg ggctttgtag
    15301 ccattggtgc ggctggtccg ccaaatatgc agttgggaag tattatcgcg acgtcatttg
    15361 cgattttatc cggtgcgtct acagaggccg cgctgaccat tgctatccct gtggcagtaa
    15421 ttggtgagtt tatgagcgtg atcatgcgca ttatcattgc tcagttttct catgtggcag
    15481 atcgggcgat agaagaagaa aattacaaaa aggctcaaaa tatccatatt tggtggtcat
    15541 ttggattaaa ctgtgttgtt tattttgtgc cgatttttct gacgatctac tttggaacag
    15601 atttagtgga tacaatggtg aacgcgattc ccgaggtaat tacaaatggc ctgacagtag
    15661 ctggaaatct gctttctgca ttgggctttt ccatgctgct cagtacgatg ctttctaaaa
    15721 agctgtttcc gtattttctg ttcggttttc tggtggtggc atattccggg ctcgatttga
    15781 tcggtctgac gctgttcgcc tgtattttag catttattat ggataaagtg gtttatggaa
    15841 gaagggagag tgcgtagata tggaagaaat gaatcagacc gtcgcagagg gcgctgtgac
    15901 aaaaaaggat ctgcgcagat tattcctgcg ttcctggact attatgtgtt cctggaatta
    15961 tgagaggcag atgcatatgg ggtttatgtt tggaatggct cccattctgg acaagctata
    16021 taagaataag cctgagaagt gtcgggaagc atatcagcgt catatggagt tttttaactg
    16081 tacaccgcag gtgacaggtt ttattatggg attggctgcc tccatggagg agcagaatgc
    16141 taaatgtgag gatggttctt tccaaccaga atcgatttct atgattaaaa ccagtctaat
    16201 gggacctttt gcgggaattg gcgacagctt tttccagggt actctgcgta tcataacatt
    16261 tggaattggc ctgtcttttg cacagcaggg caatgtatta ggaccgattc tggcagttct
    16321 tctcttcgca atcccctcaa ttatcatggg gtattatgga acggttctgg gctatcagtc
    16381 tggtaataaa tacctgacca agctgtatca ggaaggaatc atggagcggg ttatgcagtg
    16441 tgcatccatt gtgggattag ctgtagtagg aggaatggtg gcaagtatgg tcagcataac
    16501 aacaccattg gcttttcatg cggggggaac ggagctggtg cttcaggata tgctggatac
    16561 gatgttccct aagcttctgc ctcttttggc aacgctgggg atttacaggc ttattcagaa
    16621 gaaagtcaac acaaatctgc ttttgctggg aattgtgata tgtggaattg tgttgggggc
    16681 attgggaatt ttgtagtgca gggaatgtgt tagtaatcag aataatatta ttatgtaaga
    16741 actgtcgttt taacggcagt tcttaattta tgtgcgcttg ggcagatggc ctttattgcg
    16801 ttttttagaa aaatagtata ttatacctga attattcacg actttgccac gaatgtggct
    16861 gaaagccaca tagttactgt attttaactg aatattgctt ttcacctatc aagtgaatga
    16921 aaaaagagca tccatttgtg gtaaaattaa ggtgtctaat caaaataaat accaacaaag
    16981 gagcccttta tgagtattgt aaacacctta tcactagaaa gcaatagaca aattaaaata
    17041 aattttgatg gaggagattt atcctccgat gcaggattgc ttctcatcaa agaattcgta
    17101 agcaaacctg gtattgataa tcttttaggc aaagttttca agaccaacga ttctgcatta
    17161 ttcagatatc atacagatca gaaaaacctg ctccagatga tatatatgat cattgcaggc
    17221 tacttcgaag atgatacttc cgatgaactt acaaatgatc ctgtattcaa gtctgtactt
    17281 gaaaaagaca cccttgcatc acagcctacg gtatcaagat tctttaaccg tatggatgaa
    17341 gataccttaa atcagttact tatgattggc agaatactcc gaaagagagt ttatcgtatt
    17401 cagatgccac aggctgttat tctggatttg gattccactc ttcttgatgc atacggcaaa
    17461 caggaaggca gagcctttaa ctttcattat caaagcaatg gataccatcc acttgtctgt
    17521 tataatggaa tgaccggaga tctggtaaaa atccagcttc gtgatgaaac cgagtattcc
    17581 tctaccggag tggttgactt cctgcagccg atacttgatg aatacctgaa tgattatccg
    17641 gcgatccgta ttctgcttcg tggtgaca
//
LOCUS       DBMDUH010000041        17391 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_1901_length_17391_cov_4.389994, whole genome shotgun sequence.
ACCESSION   DBMDUH010000041 DBMDUH010000000
VERSION     DBMDUH010000041.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 17391)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 17391)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17391
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1901_length_17391_cov_4.389994"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="ACZPCG_05330"
     CDS             complement(<1..477)
                     /locus_tag="ACZPCG_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HIR1284530.1"
                     /translation="MLAYTYVEKGKFKLLDKPVPKLSDARDAIVRVTMSSICSSDLHI
                     KHGSVPRAVPGITVGHEMVGIVEETGKDVTKVKPGDRVTVNVETFCGTCFFCQNGYVN
                     NCTDPNGGWALGCRIDGGQAEYVCVPYADQGLNKIPDEVSDEQALFVGDVLATGFWA"
     gene            complement(590..1711)
                     /locus_tag="ACZPCG_05335"
     CDS             complement(590..1711)
                     /locus_tag="ACZPCG_05335"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004841517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88/105 protein"
                     /protein_id="HIR1284531.1"
                     /translation="MKGIDQKELEQKLNLVIEKLMHLGGPEDEADLEAGGEKIGFFRR
                     DFGIHEWDWPQGVGLYGLLKIMKIKKNKEYTDFLYHWFEDNIRQGLPSKNINTTTPLL
                     TLAEFNEECKNPEFEALCLKWADWLMNCLPRTKEGGFQHVTSANGDRQGVRLNENEMW
                     IDTLFMTVLFLNRMGQKYGRKDWIDESIHQVLMHIKYLCDRKTGLFYHGWTFNERNNF
                     GGIFWCRGNSWFTLGILDYIDMFEGTMNAGLKTFIIDTYKAQVEKLKELQDPKTGLWH
                     TVLTAPDSYVETSGTAAITAGILKGIRCGILDDSYLPCAVKAIRGILDRIDSDGTVLE
                     VSGGTGMGMDAEHYKNILIAPMAYGQSLTILALAEALNV"
     gene            1816..2682
                     /locus_tag="ACZPCG_05340"
     CDS             1816..2682
                     /locus_tag="ACZPCG_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002575114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1284532.1"
                     /translation="MEKVTYYGETDGISLEQMVRSGTFNMRVKHFHPQYEIFYIVEGE
                     RIFFFNNREYQAGAGDLILVNSNLIHMTRSANSFDQGHNRIILYISREKMKSYEALFP
                     SLHLTRFFDDYYGIYHLDEEQKRLILSLFQSIRHSLLDRSHDYRTGIDLDILSWLFKI
                     IKFVQKKHQELPLQSDDPKYLAVYRIADYLSENCERPVSLDELCTEFYLSKSYICRIF
                     KEVTGYTVSEYTNIHRIRKAKRYLEETDLSISELSHLLGFESVTYFERMFKRYTSLSP
                     LKYRKNPDSPAP"
     gene            complement(2905..4053)
                     /locus_tag="ACZPCG_05345"
     CDS             complement(2905..4053)
                     /locus_tag="ACZPCG_05345"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012516.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HIR1284533.1"
                     /translation="MVRTPKRKRRIDITQAPYLAKGDGKALNTAAIQQAIEDCKEDEM
                     VYVPAGTFLTGALTLHDHMELYLEEGAVLQGSSNPEDYLPRIPSRFEGTERECYQSLL
                     NLGVMDHRAGCTSEDVLIRGKGTISGGGRLLAERMMAAERERLKEYIHSLGDKVLECE
                     NANTIPGRARGRPIQICNAKNVRITGLTLQNGPGWNVHMIYSEQIVTDHCIFRSEDIW
                     NGDGWDPDSSSGCTIFACTFYTGDDSVAIKSGKNPEGNEIGRPCSHIRIFDCICAYGH
                     GITMGSEMSGGIEDVVIWDCDLGNSLYGLEIKGTKKRGALCLRYLKNASLRIRIFWED
                     IWVRTGSTSHAGALSWRVLTKRGMRSGGLYFAGFVLAARSFKLSRKFL"
     gene            complement(4071..4265)
                     /locus_tag="ACZPCG_05350"
     CDS             complement(4071..4265)
                     /locus_tag="ACZPCG_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284534.1"
                     /translation="MENKRLHIEATEEAITVYWEKMAGEQGLRYRIYLNGTQAAETEK
                     THGTLEGLKGQKRTGNPKKN"
     gene            complement(4271..6313)
                     /locus_tag="ACZPCG_05355"
     CDS             complement(4271..6313)
                     /locus_tag="ACZPCG_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 115 family protein"
                     /protein_id="HIR1284535.1"
                     /translation="MEKITLDSRIYFDQGNLAVCLAVENLKRDMSRVLCPGEDQMMRG
                     GVNGTPIRLVNAELPPEMWELELKKGEFLLKAGSPLGFVYGIYEISRSFLGIQPFWFW
                     NDQAFETKPFAEIPDGFYKRSEPFRVKLRGWFVNDEVLLHTWKVNRDPAEPWRMVFEA
                     LLRLGGNMVIPGTDRNAHRYRKLASDMGLAITHHHAEPLGAEMFARAYPALNASFAEH
                     SDKFYTLWEKAVKEQKDMNVVWNLGFRGQGDCPFWESDPRYDTDEARGELMSRLIRIQ
                     YEMVKKENPQALCCTNLYGETMELYKKGYLQLPEDVIHIWADNGFGKMVSRRQGNHNP
                     RILALPEKNSEKKHGIYYHASFYDLQAAAQLTMLPNSPEFVEKELKQVLEQGADDYWL
                     INCSNVKPHVFILDEIAQLWKTGTLSEDWAKGYAARYFGEKGAAEIAARYLEFSGCAV
                     KYGPNEDDRAGEQFANHVPRILMTQFIQDKNSCAQAFLWAFRADTLKEQVRWYEALCR
                     QAVRSYGRYLEACKKTALGLSGRARRLFEDSLLLQADILHGCYEGALEICKSLLCGFG
                     GDNLGGFYYAGKAAESYRNTDGRMRAREHGKWHGFYENECQADIKQSSWVAQTLMGYF
                     RVLGDGPHFYQWQREFSDTEENKRVVLILNMENHLTHEELYERMKERWEENETDGK"
     gene            complement(6390..7718)
                     /locus_tag="ACZPCG_05360"
     CDS             complement(6390..7718)
                     /locus_tag="ACZPCG_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002605270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1284536.1"
                     /translation="MGLGFVAAAATLTACGQNEETTAAASFGQEAQASENGDSSGEIT
                     FCWWGGDSRHEATEKAVEAFMKKYPEIKVTTEYGAWSGWEQKQSLALSSGTAPDLMQI
                     NWNWIDNYGKNGTTFLDLNQFSDIIDLTQFPEEVLEQCTVDGKLMAIPVSNTGCLFFW
                     NKATFDTIGCEIPTDKESLMEAGRKFKEYGDEYYPIVFDGGYSRMLLTVYYLESVYGR
                     PWVEDGELQYSEKEVAEALTFITELEEGHVIPTLAVTNGDMVDSTDKNPKWIDGKYAG
                     VYSWDNTIIKMEDAIQESVNVPNQELVVGDFLKFGDYQGGFTKVSMAFAIPATCENPE
                     AAATLINYLLNDPEGVELCGLERGVPLSEAGANHVMEKGLGDPRVLEANKAVLNYSKF
                     PLDPKFDSSNLMASPDGVYEKVFGKLSAGECDAAQAAKELVDGVKETLSE"
     gene            7885..8724
                     /locus_tag="ACZPCG_05365"
     CDS             7885..8724
                     /locus_tag="ACZPCG_05365"
                     /inference="COORDINATES: protein motif:HMM:NF014376.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1284537.1"
                     /translation="MKASKYKMDSEFRMEIDRQIRHAMPEMHYHDFYEIYIQDLGTHE
                     HIVSSSFFKLSPHDVMLLKPHILHQSISLDLHTRTVIYFTDKFLHDFFSEQAVKELMR
                     PFQYEVLSLSPEHYYRAAALVNELSHADFYHLAPSTYLNVAELLVIFAKSIEEHPGLC
                     SKKQNPDPLQHSPAIQSPLITYVHENFLTLTNLEEIADTFFITPSHMCRTFKKLTGFT
                     IFQYINMLKIQKACELLHNTQKSITDIAMECGFNSAMYFGRTFKATLGLTPTEYRGMN
                     DCG"
     gene            complement(8738..9016)
                     /locus_tag="ACZPCG_05370"
     CDS             complement(8738..9016)
                     /locus_tag="ACZPCG_05370"
                     /inference="COORDINATES: protein motif:HMM:NF025120.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1284538.1"
                     /translation="MGNSMDYIYTSGEQKVIIEAEQKNTRRSVIEQYVQCRKMQGMTQ
                     AELAKRTGIPRSNITRFESGSYNPSLELLVRIAAALGMNLQVQLTAKE"
     gene            complement(9006..9743)
                     /locus_tag="ACZPCG_05375"
     CDS             complement(9006..9743)
                     /locus_tag="ACZPCG_05375"
                     /inference="COORDINATES: protein motif:HMM:NF040212.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5839 family protein"
                     /protein_id="HIR1284539.1"
                     /translation="MNESLYVYLNLDTEKEQESEELIRRMDQLLLTAGMKYSGISNMY
                     IPVDIQKRDRAVFRAEELLRSTDWLKGILAYSAVGTLTNACPIEEIQTDSMQDPMPGK
                     LRYYEQYYQKTNHLPHGIVVDENRQLRDGYISYLLAKKYHARADVCEMVSGQPLRKLV
                     TGVHVVFRDGKWIKKTNKGYNWIYTKKAPVIPGDILLVDTKRGTSFICVSRITYIAGQ
                     EFCSNYKKVRKHMKIHMEEAKDTNYGK"
     gene            complement(9853..11631)
                     /locus_tag="ACZPCG_05380"
     CDS             complement(9853..11631)
                     /locus_tag="ACZPCG_05380"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HIR1284540.1"
                     /translation="MDQKRENLLNLALSASEEERLKSENLEIGYDPEDKTWELILRYS
                     GNLNRLRELSDQGVTVRELFNGYAIARVPQGLLDQVSGIREIQYIEKPKRLFFAMDMA
                     KAASCLLGIQPGRREEGRSSGTEPGGHTGGLEHGLTGKGVILGIIDSGIDYLHPDFRN
                     EDGSTRILYLWDQDQGQVYDREDINRALAAYGEQNDRRAALNIVPSVDVSGHGTAAAA
                     IAAGNGRGSDGVYRGVAYESELIAVKLGTSPKDSFPRTTQLMEGLDFILKRAILLERP
                     VAVNVSFGNTYGSHDGTSLLETFMNEASNYSRNVIVTGTGNEGASAGHTAGQLVMGER
                     ERIELSVAPFETSFSVQIWKSYADQFSILLTAPDGRSLGPIEERLGPQRLENGNTRIL
                     IYYGKPSPYSRAQEVYFDFIPLKDYTDSGIWTFQLTPIELVTGHYDFWLPSGRILNPS
                     TRFLRPVPETTLTIPSTASGVISVGAYNDSNLAYADFSGRGFTRQTRQVKPDLAAPGV
                     DIITAKAGGGYEAVTGTSFAAPFVTGSAALLMQWGIVDGNDPFLYGEKVKAYLIRGAR
                     HLPGYTEWPNERVGYGALCVRESLPV"
     gene            complement(11730..12860)
                     /locus_tag="ACZPCG_05385"
     CDS             complement(11730..12860)
                     /locus_tag="ACZPCG_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002600519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HIR1284541.1"
                     /translation="MPYGIDNETEEWFKGLEKKLYAKLKTQCLRLGSIMPYVPEHGCY
                     YDMGARELTAWTNGFYAGMLWQMYHAFKEEEFRRAAEGIEERLDAALEEFVKLDHDVG
                     FLWLHTAVADWRLTGNETSRKRGLHAAGILAGRYNPAGHYIRAWNGVRQGVAIVDCLM
                     NLPLLYWAAQENESPAWKQIAIEHTEMALRYILRPDGSSNHMVDFDPETGEYKDNPGG
                     QGYESGSSWSRGQSWAIYGMALAYRYTKNQAYLDAAKRSAHYFMANVSLNDYIPLLDF
                     RAPKEPVYIDTTAGACAACGLLELSEHVGEYEKPLYVQSALRCLKAVQAFCEWDPDKD
                     SILDGGSARYDRASDRQVPIIYGDYFLTEAILRFREKTFLIW"
     gene            complement(12844..13590)
                     /locus_tag="ACZPCG_05390"
     CDS             complement(12844..13590)
                     /locus_tag="ACZPCG_05390"
                     /inference="COORDINATES: protein motif:HMM:NF045254.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 C-terminal domain-containing protein"
                     /protein_id="HIR1284542.1"
                     /translation="MALPDDHPFWQAEEESLPSLEPVQCIRECDMLICHRPHEAVALT
                     AGQYPTMEHPMAAEKYAKFAYSTVFGYCVSRSFHCLEQAGTDSMLAFYVHGMYYVRRR
                     CLEHRVTETEVYARWSPIEGIEVETWLILAEGGHLRRHRIVSRLSCEAYDCGFSYPDC
                     MEQTSQTEEEHMAAVWDENGESRLSASMGQGTVIRPFSNMNLIFPNVRIPAVHLAIEP
                     GVTEFESMVTASFTQKDIVIGGGNCYALRD"
     gene            complement(13603..14507)
                     /locus_tag="ACZPCG_05395"
                     /pseudo
     CDS             complement(13603..14507)
                     /locus_tag="ACZPCG_05395"
                     /inference="COORDINATES: protein motif:HMM:NF021534.6"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
     gene            complement(14455..14577)
                     /locus_tag="ACZPCG_05400"
     CDS             complement(14455..14577)
                     /locus_tag="ACZPCG_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284543.1"
                     /translation="MKKKADYQKMLLKMIRPLTRHYTDDGSGLYLGAFSAGYGN"
     gene            complement(14690..15004)
                     /gene="rhaM"
                     /locus_tag="ACZPCG_05405"
     CDS             complement(14690..15004)
                     /gene="rhaM"
                     /locus_tag="ACZPCG_05405"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019318 - hexose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HIR1284544.1"
                     /translation="MIRKAFKMFLYPGMEEEYEKRHNLLWPEMKEMIHAHGGHNYSIY
                     LDRETHVLYGYIEIEDEALWAASADTPINRKWWDFMADIMETNEDNSPVSVDLAPVFH
                     LD"
     gene            complement(15018..15842)
                     /gene="rhaD"
                     /locus_tag="ACZPCG_05410"
     CDS             complement(15018..15842)
                     /gene="rhaD"
                     /locus_tag="ACZPCG_05410"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002575115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HIR1284545.1"
                     /translation="MRFLDAEFVQGFIRMANDGWEQGWHERNGGNISYRVKDEEVESV
                     KEELEPKEWRPIGTTVPALGGEYFLVTGSGKYLRNVIIKPEDSICMIELDQAGENYRI
                     VWGLVNGGKPTSELPSHLMNHEVKKIATNGAYRVIYHAHTVNVIALTFVLPLKDEVFT
                     RELWEMATECPVVFPAGIGVVPWMVPGGRDIAVATSELMKQYDVAIWAHHGTFAAGED
                     FDITFGLMHTVEKSAEILVKMLSMRPDKLQTITPQNFRDLAKDFHVTLPEKFLYEK"
     gene            complement(15857..17116)
                     /locus_tag="ACZPCG_05415"
     CDS             complement(15857..17116)
                     /locus_tag="ACZPCG_05415"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006861372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019318 - hexose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HIR1284546.1"
                     /translation="MNQTERYESAKEMYKSLGIDTDQVIQTLKNIPVSLHCWQGDDVK
                     GFDQDGPLTGGIQTTGNYPGRARTPQELMDDMEMAMSLMPGKKKLNLHASYAIFEEGE
                     NADRDQLEPKYFAKWVEFAKKHNMGIDFNPTFFSHEKVKDGQTLSSPDKETREFWIRH
                     GKACIRISQYFAQETGVPCVMNIWTGDGFKDVPADRMGPRMRYKESIEEILSEPYDPE
                     LVKPCVESKVFGIGVESYTAGSAEFALSFAATHKGVLPLMDNGHYHPTEMVSDKIPAL
                     LCFFPEIALHVTRPVRWDSDHVVLFDDETKEIAKEIVRNNALDRVYIALDYFDASINR
                     ISAWAVGFRSFQKALLSALLTPAALLKELQDENRMTELMMMQEELKLYPLGDVWNEYC
                     RQCGVESDQSWFEKIKIYEKDVLSQRK"
     gene            complement(17147..>17391)
                     /locus_tag="ACZPCG_05420"
     CDS             complement(17147..>17391)
                     /locus_tag="ACZPCG_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HIR1284547.1"
                     /translation="EGLTGSHYNTICIVGGGSNAEYLNRLTAKKSGRRVLAGPGEATA
                     IGNLMAQMIQKGEFDTLEQARRCVGTSFSIHEYMPE"
BASE COUNT         4247 a         4844 c         3404 g         4896 t
ORIGIN      
        1 tgcccagaag ccggtcgcca gcacatcccc cacaaaaaga gcctgctcat cactgacctc
       61 atccggaatt ttatttaatc cctgatccgc atagggaaca cacacatatt ctgcctgccc
      121 gccgtcgatc cggcatccca gggcccagcc gccgtttgga tcggtacagt tgtttacata
      181 tccgttttga cagaaaaaac aggttccgca aaacgtttcc acatttaccg ttaccctgtc
      241 tcccggcttt accttggtta catcttttcc tgtttcttcc acgattccca ccatctcatg
      301 gcctactgta attcccggaa ctgcccgggg tacagaaccg tgcttgatat gaagatcact
      361 gctgcagatg ctggacatgg taacacgcac aatcgcatct cttgcatctg aaagttttgg
      421 aaccggtttg tcaagaagtt taaattttcc cttttccaca taggtatacg caagcatgaa
      481 acgacctcct gatattttga aataactgcc ttcattttac catactgcct gtgctttcgc
      541 aatcaggaag cccttgggtt cctgtattca tctgcctgta aactccggtt taaacgttca
      601 atgcttctgc cagggccagt atggtaagag actgtccata tgccatagga gcaatcagga
      661 tatttttgta atgttctgca tccatcccca ttcctgtgcc tccggacact tcaagaaccg
      721 ttccgtctga atcaatacgg tctaaaattc cccgaatggc ctttacagcg catggaagat
      781 aggaatcatc cagtatgccg cagcggatcc ccttgagaat ccccgctgta atcgctgcag
      841 tacctgaggt ttccacatag ctgtccgggg ctgtgagaac cgtatgccac agtccggttt
      901 tgggatcctg aagctcctta agcttctcca cctgagcctt ataggtatct atgataaatg
      961 tttttaagcc tgcattcata gtaccctcaa acatatcaat gtaatccagg attcccaggg
     1021 taaaccagct gtttccccgg caccagaata tgcctccgaa attattcctt tcgttaaaag
     1081 tccagccatg ataaaaaagg cccgttttcc gatcacacag atatttgata tgcatcagca
     1141 cctggtggat ggattcatca atccaatcct tacggccata cttctgtccc atacggttca
     1201 aaaagagaac cgtcataaac agcgtatcga tccacatctc attttcattt aatcttacac
     1261 cctgacgatc tccatttgca ctggtcacat gctgaaatcc gccttctttc gtccttggca
     1321 gacaattcat cagccagtct gcccacttta agcaaagggc ctcaaattcg ggatttttac
     1381 actcctcatt aaactctgcc agagtcagaa gcggagtggt tgtattgata tttttagagg
     1441 gaagcccctg acggatattg tcttcaaacc agtgatataa aaagtccgta tactctttat
     1501 ttttctttat tttcatgatt ttcaacaggc cgtaaagccc gactccctga ggccagtccc
     1561 actcatggat gccgaaatcc ctacggaaaa atccaatctt ttcaccgcct gcctccaaat
     1621 ccgcttcatc ctccggtcct cccagatgca tcagcttttc aatgaccaaa tttaacttct
     1681 gttctaattc tttctgatcg atccccttca tttttcctcc atccaatccg gtatgccttt
     1741 tcaaaaataa gaacaccact ggattttttc attctcatat tatataattt taaccgttga
     1801 aaggatattg atcgcatgga aaaagtgact tattacggag aaacagacgg tatttccctg
     1861 gaacaaatgg ttcggtctgg aacctttaat atgcgtgtaa agcatttcca tccccagtat
     1921 gagatttttt atatcgtcga gggggaacgg atcttctttt ttaataacag agagtaccag
     1981 gcaggagccg gggatctgat tcttgtcaat tccaatctga tccatatgac ccgctctgca
     2041 aacagctttg accaggggca taaccggatc atcctctata tttcccggga gaaaatgaag
     2101 tcctatgaag ctttgtttcc ctctctgcac ctgacccgtt tttttgatga ttactacggc
     2161 atctaccacc tggatgagga acagaaacgg ctgattttat ctctctttca aagcatccgc
     2221 cacagtcttt tagaccgctc ccatgattac cgcacaggca ttgatctgga tattttatcc
     2281 tggcttttta agatcataaa attcgtccaa aagaagcatc aggagcttcc cctccagtct
     2341 gacgatccca aatatctggc tgtctaccgc atcgcagatt atctctctga aaactgcgaa
     2401 cggcccgttt ctctggatga actctgtacg gaattttatc tcagtaaatc ctatatctgc
     2461 aggatattta aagaggtcac cgggtatacc gtcagcgaat ataccaacat tcaccgcatc
     2521 cgcaaagcca agcgttatct ggaagaaacg gatctgagca tatccgagct ctcccacctg
     2581 ctgggctttg aaagcgtgac ctattttgaa cggatgttca aacgctatac ctctctgtca
     2641 cccttaaaat accgaaaaaa tcccgacagt ccggctccct gacagaaagc ctcccatccc
     2701 cagcgcatca tttcaccatt ccatgctctc agcagacagg ctagaaatgc ctgtctgccg
     2761 gtaacagcta caatgtatca cagacatagg acagattctt cttccggatt gacatatttt
     2821 tctctttttc aaactatatt gtacccgcag agtcggatgg agaaatcgtg cttacatcct
     2881 ccaggctgat ttctttgcaa tatttcataa aaatttccgg ctgagtttga aactcctggc
     2941 agccaaaacg aatcctgcga aatacaatcc cccggatctc atacccctct tcgtcaaaac
     3001 cctccagctc aatgcccctg catggctggt actccctgtc cttacccata tatcttccca
     3061 aaatatccgt atcctcaaag aggcattttt caaataccgg aggcacaggg cgcctctttt
     3121 tttcgttccc tttatctcaa gcccatagag cgaatttcca agatcgcagt cccatataac
     3181 cacatcctca attcctcccg acatctcgct tcccatggta attccatggc cataggcaca
     3241 gatacagtca aaaatcctga tatgggagca ggggcgtccg atctcatttc cttccgggtt
     3301 ttttcccgat ttaatcgcca ctgaatcatc tcccgtataa aaggtgcagg caaatatggt
     3361 acatcctgat gaggaatccg gatcccagcc atccccgttc cagatatctt ccgagcggaa
     3421 tatgcagtgg tcggtaacaa tctgttccga atatatcata tgtacgttcc atccgggtcc
     3481 attttggaga gtaagccctg taatccttac attttttgca ttgcatatct ggatcgggcg
     3541 tcctctggcg cgccccggta tggtatttgc attttcacat tccagcacct tatctcccag
     3601 agaatggata tattccttca gccgttcccg ttccgccgcc atcatgcgct ctgccagaag
     3661 ccggccgccg ccggatatgg ttccttttcc gcggatcaaa acatcctcac tggtacatcc
     3721 cgcccgatgg tccatcacgc ccagatttaa aagactctga taacactccc tctctgtgcc
     3781 ttcaaaacgg cttggaatcc tgggaaggta atcctcagga ttactgcttc cctgaagaac
     3841 agctccctcc tccagataaa gctccatatg atcatggagc gtcagggccc ctgtcaaaaa
     3901 ggttcctgcg gggacataaa ccatctcatc ctctttgcag tcctctatgg cctgttgtat
     3961 ggctgctgta tttaacgcct ttccatcgcc ctttgccaga tagggagcct gggtaatgtc
     4021 aatccggcgt tttcttttcg gggtacgtac catacagctg ctttcccaga tcagttcttc
     4081 tttgggttcc ccgttctttt ctgtcctttt aatccctcta atgttccatg tgttttctct
     4141 gtttctgccg cctgtgttcc attcagataa atccgatacc gcagtccctg ttctcctgcc
     4201 attttttccc agtatacggt aatggcttcc tccgttgctt ctatatgcag acgtttattt
     4261 tccatcggtt ttattttcca tcggtttcat tttcctccca tctttccttc atccgctcat
     4321 acagttcctc atgcgtcaga tggttttcca tatttaaaat caggactact ctcttatttt
     4381 cttctgtatc cgaaaattcc ctctgccact gataaaaatg aggcccgtct cccaaaactc
     4441 tgaaatatcc catcagggtc tgcgcgaccc agctgctctg cttgatatcg gcctggcact
     4501 cattttcata aaagccatgc cattttccat gctctctggc ccgcatcctc ccatctgtat
     4561 tccggtatga ttccgccgcc tttcctgcat agtaaaaacc tcccaggtta tctccgccaa
     4621 aaccgcacag caggcttttg catatctcca gagcaccctc atagcagcca tgtaaaatat
     4681 ccgcctgcaa aagcagagaa tcttcaaaca gcctgcgggc ccgtccggag agcccaaggg
     4741 cggttttttt gcacgcttcc agatagcgtc catacgaacg gactgcctgt ctgcacagag
     4801 cctcgtacca cctcacctgc tcttttaagg tatccgctct gaaagcccac aaaaatgcct
     4861 gcgcacagct gtttttatcc tgtataaact gtgtcataag gatcctcggt acatgattgg
     4921 caaactgttc tcctgcccgg tcatcctcat ttggcccgta tttgacagca cagccggaaa
     4981 actccagata acgggccgca atttcagcag ctcccttttc accaaaatag cgggccgcat
     5041 acccctttgc ccagtcctct gacagtgttc ctgttttcca aagctgtgcg atctcatcca
     5101 gtataaatac atggggcttt acattggagc agttgatcag ccagtagtcg tcagccccct
     5161 gctccagcac ctgctttaac tccttttcca caaactccgg cgaattgggc agcatcgtca
     5221 gctgggcggc agcctgaagg tcataaaaag atgcatggta atatataccg tgtttttttt
     5281 cgctgttttt ttccggaagg gccaaaatcc tgggattgtg gtttccctgc ctgcgggaaa
     5341 ccatttttcc aaatccattg tccgcccata tatggatcac atcctccggg agctgcagat
     5401 atcccttctt atacagctcc atcgtttcac cgtagagatt cgtacagcac agcgcctgag
     5461 gattttcctt ttttaccatt tcatactgta tccggatcag gcggctcatc agctctcccc
     5521 tggcttcatc cgtatcatac cggggatccg attcccagaa ggggcagtcc ccctggcccc
     5581 ggaatcccag attccatacc acattcatat ccttctgttc tttcacagcc ttctcccaca
     5641 gcgtataaaa tttgtcgctg tgctccgcaa aggaggcatt cagagccgga taggcacggg
     5701 caaacatctc tgcccctaaa ggctccgcat gatgatgcgt aatcgccagt cccatgtcag
     5761 aggccagttt tctgtagcgg tgtgcattcc tgtctgtgcc cgggatcacc atatttcccc
     5821 ccagacgcag cagggcttca aacaccatgc gccagggctc tgccggatcc cggtttacct
     5881 tccatgtatg taaaagaacc tcatcattta caaaccagcc tctcagtttt acccgaaagg
     5941 gctctgatct tttatagaac ccatccggga tctctgcaaa gggcttcgtt tcaaatgcct
     6001 ggtcattcca gaaccagaag ggctgtatgc ccaaaaagct gcggcttatt tcataaatac
     6061 cgtatacaaa gccaagagga gatccggcct tcaaaagaaa ctcccccttc tttaattcca
     6121 gctcccacat ctcaggaggg agttctgcat ttacaaggcg gatgggggta ccgtttacgc
     6181 ctcccctcat catctgatcc tctcccggac acagaacacg gctcatatcc cgctttaaat
     6241 tttcaaccgc aagacacact gccagattgc cctgatcgaa atatatcctt gaatccagtg
     6301 ttattttttc catctttctt tccttcttct ttctatctga caatccgggc ctgccttcac
     6361 aaacggaaag caggccctgc cttttacttt tattctgata atgtttcctt tacgccgtca
     6421 accagttctt ttgccgcctg tgcagcgtca cattctcctg ctgacagctt tccgaatact
     6481 ttttcataaa cgccatccgg agatgccatc aaattgctgg aatcaaactt gggatccagc
     6541 gggaatttgg agtaattcag cacagcctta tttgcttcca gcaccctggg atcgcccagt
     6601 cccttctcca tcacatggtt tgcccctgct tcagataagg gcactccccg ctccagaccg
     6661 caaagctcaa cgccttcagg atcatttaaa agataattga tcagcgtagc tgctgcttcg
     6721 gggttttcac aggtagcagg aatcgcaaat gccatagaaa cctttgtaaa tccgccctga
     6781 taatctccga atttgaggaa atctcctaca accagctcct ggtttgggac atttacactc
     6841 tcttgaattg catcctccat cttaatgatt gtattatccc aggaatatac gcccgcgtac
     6901 tttccgtcaa tccatttagg atttttatca gtagaatcca ccatatcgcc atttgtcact
     6961 gcaagtgtcg ggatcacatg accctcttcc agttctgtga taaacgtaag ggcttctgct
     7021 acctctttct cagaatactg aagctctcca tcttcaaccc agggtcttcc gtatacagat
     7081 tccagatagt ataccgtaag aagcatacgg ctgtaaccgc cgtcaaatac aatgggataa
     7141 tattcatccc catattcctt aaactttctt cccgcttcca tcaaagattc cttatcggtt
     7201 ggaatctcgc atccgatggt atcaaaggtt gccttattcc agaaaaacag acagcccgta
     7261 ttggaaaccg ggatcgccat cagctttccg tctaccgtac actgctccag cacctcctcc
     7321 ggaaactgcg tcagatcaat gatatcgcta aactggttta aatccagaaa tgttgtgccg
     7381 ttctttccat aattgtcaat ccagttccag ttaatctgca tcagatcagg cgctgtgccg
     7441 cttgataatg ccagagactg cttctgctcc cagccgctcc atgctccata ctccgttgta
     7501 accttaatct ccggatattt cttcataaag gcctctactg ccttttctgt agcctcatgc
     7561 cgggaatcac cgccccacca gcaaaatgtg atttccccgg aagaatctcc attctcagac
     7621 gcctgagcct cctgcccaaa agaggctgct gcagttgtct cctcattttg tccgcaggca
     7681 gtcaaagttg ctgcagctgc cacaaatccc aaacccatta tccatcgttt tgtcactgtt
     7741 ttttacctcc tgattccctt attgcaatta tattgctgaa tatgatagaa tcattataga
     7801 gcaatcatat gcagaatgta tgattgattt tgctcttttt gcacttatta atacaatatt
     7861 gatatcaatt taaaaggagt taccatgaag gcaagcaaat ataaaatgga ttccgaattc
     7921 aggatggaaa ttgaccggca gatccggcac gctatgccgg aaatgcacta tcacgatttt
     7981 tatgaaattt atattcagga tctgggtacc catgagcaca ttgtaagcag cagctttttt
     8041 aaactgtctc cccatgatgt tatgctgctg aaaccccata tccttcacca gtctatcagc
     8101 ctggatttac atacacggac tgttatttat tttacagata aatttctcca tgactttttc
     8161 tcagagcagg ctgtaaagga actgatgcgc ccctttcagt atgaggtttt atctctgtcc
     8221 ccagagcatt attatcgtgc agctgccctt gtaaatgaac tcagccatgc agatttttac
     8281 catctggctc catccaccta tctgaatgtg gccgaactgc tggttatctt tgcgaaatcc
     8341 atcgaggagc atcccggact gtgctcaaaa aagcaaaatc ccgatccttt acagcattcc
     8401 cctgctatcc agtctcctct gattacctat gtccatgaaa attttctcac gctgacgaat
     8461 ctggaggaaa ttgcagatac attttttatc actcccagcc atatgtgccg aacctttaaa
     8521 aagcttacgg gatttactat ttttcagtat atcaatatgc tgaaaataca gaaagcctgt
     8581 gagctgcttc ataataccca aaaaagcata actgatatcg ccatggaatg cggctttaac
     8641 tctgccatgt attttggccg gaccttcaaa gctactctgg ggcttacgcc tacagagtac
     8701 cgggggatga atgactgcgg ataatcctat ctgccgttca ttccttcgct gtcagctgca
     8761 cctgtaagtt catccccagt gctgccgcaa tccgtaccaa cagctccaga gacgggttgt
     8821 aactgccgct ttcaaatctt gtaatattgc ttctcgggat tcctgttctt ttcgcaagtt
     8881 ccgcctgtgt cattccctgc attttccggc attgcacata ctgttcaata acagaacgtc
     8941 tggtattttt ctgctccgcc tctattatga ctttctgttc gcctgatgta taaatataat
     9001 ccatactatt tcccataatt tgtatctttc gcttcttcca tgtgtatttt catatgtttt
     9061 ctgaccttct tatagtttga acaaaactcc tgtccggcaa tataggtaat tctgcttacg
     9121 catataaaag acgttcctct ctttgtatcc accaacagaa tatctcccgg aataaccggc
     9181 gccttttttg tgtaaatcca attatatcct ttattcgtct tctttatcca tttcccgtca
     9241 cgaaatacca catgaacccc cgtaaccagc tttcttaacg gctgtcccga taccatttcg
     9301 cagacgtctg cgcgtgcatg atacttttta gccagaagat atgatatata accatctctg
     9361 agctgcctgt tctcgtctac tacaattccg tgcggcaaat gattcgtttt ctgataatac
     9421 tgctcataat accttagctt tcccggcata ggatcctgca tactgtctgt ttgaatttct
     9481 tcgatcggac aggcattggt cagcgtcccc acagcactgt atgccagaat tcccttcagc
     9541 caatccgtgc ttctcaatag ctcctctgcg cgaaaaaccg cccggtcacg tttctgtatg
     9601 tccaccggaa tgtacatatt tgaaataccg gagtacttca tgcctgcggt caaaagcagc
     9661 tgatccatcc gccggatcag ttcttcactt tcctgttcct tttccgtatc cagatttaaa
     9721 tatacataca atgactcatt cattaaaaat cctccttttt cacaggaacc atatatgata
     9781 cattaaatgt atcgtatatg attctttttt tcaagaagaa cgcttgattt ttagtatata
     9841 agaaacgtat ttttaaaccg gcagactctc tcttacgcac agcgccccat accccaccct
     9901 ctcattcggc cattctgtat aaccaggaag atggcgggcg ccgcggatga ggtaggcctt
     9961 taccttttcc ccataaagaa aggggtcgtt tccgtctaca atgccccact gcatcaaaag
    10021 cgccgcgctc cccgtgacaa atggggctgc aaaggaggtt cctgtcaccg cttcatatcc
    10081 ccctcctgct tttgcggtga tgatatcgac tccgggagca gccagatccg gctttacctg
    10141 cctggtctgc ctggtaaagc cacggccgga aaaatccgca tatgccagat ttgaatcatt
    10201 atatgctcct acagaaataa cccctgatgc tgtggaagga atcgtaagtg ttgtctccgg
    10261 caccggacga agaaaacggg tggagggatt taaaatccgg cctgaaggaa gccagaaatc
    10321 ataatggcct gtgacaagct ctattggtgt cagctgaaag gtccagatcc cactgtcggt
    10381 gtaatccttc aaaggaataa aatcaaagta aacctcctga gcccggctgt agggactggg
    10441 ctttccataa tagatcagaa tacgtgtatt tccattttcc agtctttgag gccccagccg
    10501 ttcttcaatc ggccccaggc tccgtccatc aggcgctgta aggagaatgg aaaactgatc
    10561 tgcataggac ttccagatct gtacactgaa actggtctca aagggagcta cagacagctc
    10621 aatcctttcc ctttctccca taacaagctg tcccgccgtg tggcctgctg acgctccttc
    10681 gtttcccgta cctgtgacaa ttacattccg gctgtaattg gaggcttcat tcataaaggt
    10741 ttccaggagg ctggttccgt catgggaacc ataggtattt ccaaagctga cattaacagc
    10801 caccgggcgc tcaagcagaa tggctctctt taatataaaa tccaggcctt ccataagctg
    10861 agtggttctg ggaaaactgt ccttgggaga agttcccagc tttactgcaa taagttcgct
    10921 ttcataggcc actccccggt aaactccatc gcttccccgt ccgtttcccg cagctatggc
    10981 tgcagctgcg gtgccgtgtc cgctgacatc cacagaaggt actatgttaa gagccgctct
    11041 cctgtcattt tgttctccgt atgctgccag cgcccggttt atatcctctc tgtcatagac
    11101 ctgtccctga tcctgatccc acagatacaa aatccgggta ctgccgtctt catttctgaa
    11161 atccggatga agataatcga ttccagaatc aatgattccc aggatcaccc cttttcccgt
    11221 aagcccatgc tccaggccac ctgtatgtcc gcccggctct gttccagatg acctgccttc
    11281 ctctcttctc cccggctgaa tccccaaaag acaagaggct gcctttgcca tatccatggc
    11341 aaagaacagc ctctttggtt tttcgatata ctgaatctca cgaataccgc tgacctggtc
    11401 taagagcccc tggggaactc tggcaatggc atatccgtta aaaagttctc tcaccgtgac
    11461 tccctgatcc gaaagctctc tcaggcggtt cagatttccc gaataacgca ggatcagctc
    11521 ccatgtttta tcttccggat cgtatcctat ttccagattt tctgatttaa gacgttcttc
    11581 ctctgaagca cttaaagcca gatttaaaag attttcccgc ttctgatcca ctacatccaa
    11641 agacatattc ctcccggaag aatcatcccc tcagcagggc ttttttccct ctgagtctat
    11701 gtcccgggca ttcgtattat acctattttt taccagatca aaaatgtttt ttctctaaaa
    11761 cgcagaatag cctctgtaag gaaataatcg ccgtaaataa tcgggacctg gcggtctgag
    11821 gccctgtcat accgtgcaga acctccgtcc aggatcgaat ccttatcagg atcccactca
    11881 caaaatgcct gaaccgcctt caggcatctt aaagcggatt gtacataaag cggcttttca
    11941 tattctccca catgctctga gagttctaaa aggccgcagg ccgcacaggc tccggcagta
    12001 gtgtcaatat aaaccggctc cttcggcgcc ctgaaatcaa gaagggggat atagtcattt
    12061 aaagaaacat ttgccataaa ataatgagcc gaccgcttgg ctgcatccag ataggcctga
    12121 tttttcgtat aacggtatgc cagagccatc ccgtaaatgg cccaggattg gcctctgctc
    12181 caggaggagc cgctttcata tccctgtcct ccgggattat ctttatattc ccctgtttcg
    12241 ggatcaaaat ccaccatatg attactggaa ccgtcaggcc gcagaatata gcgcagggcc
    12301 atttccgtat gctctatggc aatctgcttc caggcaggag actcattttc ctgtgctgcc
    12361 caatataaaa gcggcagatt cataagacag tccacaatgg caactccctg ccgtactcca
    12421 ttccaggccc ggatataatg tccggccgga ttgtaccttc cggccagaat cccggctgca
    12481 tggaggcctc tctttctgga ggtttcattg cctgtaagcc tccagtcggc taccgccgtg
    12541 tgaagccaca gaaaacccac atcatgatcc agttttacaa attcctcaag ggcagcgtcc
    12601 aggcgctcct ctatcccctc tgcggccctg cggaactctt cctctttaaa tgcgtgatac
    12661 atctgccaca gcataccggc ataaaagccg ttcgtccagg cagtcagttc tctggccccc
    12721 atatcatagt aacagccatg ctccggcaca tagggcatga tgctcccaag cctcaggcac
    12781 tgggtcttaa gctttgcata caactttttc tccaggcctt taaaccattc ctctgtttca
    12841 ttatcaatcc cgtaaggcat agcagtttcc tcctccgatc acaatatcct tctgtgtaaa
    12901 agaagctgta accatacttt caaattccgt aacgcccggc tcaatagcaa gatgtaccgc
    12961 aggaatacgc acatttggaa aaatcaggtt catattggaa aaaggacgga tcaccgttcc
    13021 ctgacccatg gaagcactca gacggctttc accattttca tcccacactg ccgccatatg
    13081 ctcctcttct gtctggctgg tctgctccat gcagtccgga taggaaaatc cgcagtcata
    13141 ggcctcacaa gacagccggc ttacaatcct gtgcctgcgc agatggcctc cctctgccag
    13201 aatcagccag gtttccactt caattccctc tatgggactc catcttgcat aaacttccgt
    13261 ttctgttacc ctatgctcca gacacctgcg gcgcacatag tacattccat ggacataaaa
    13321 tgccagcata ctgtctgtcc cggcctgctc cagacaatgg aaggaacggg atacgcagta
    13381 tccaaacacg gtagaataag caaacttggc atatttttcc gccgccatgg gatgctccat
    13441 ggtaggatac tgtccggctg taagagccac tgcctcatga ggcctgtggc agatcagcat
    13501 atcgcattcc cggatacatt gaaccggctc aagggaagga agagactcct cctctgcctg
    13561 ccagaaggga tgatcatccg gcagagccaa aaatacaaag gcctttaagg cccagtaggg
    13621 agaaccggga gcattatacg tctcagacat aagaaggttt ggataggtat aacctaccgt
    13681 caggattcct ccattatcaa aaatcggctg cttcatccag taatccagat gacgggccac
    13741 aatccccttc atcacgcccc aggaaataca ctccactcct gccagggcac aggcggaaaa
    13801 aaaggctgcc tgtgcaaacc ggtaagtcag agaacggcca aagggcaggg cctttccctc
    13861 atcatcaaac cagtagataa agctctctga aaatgtcttt gcgcgttcct taaaaagagc
    13921 cgtatgctcc ggatcttcct tttccataaa ccgtgcatac agcagacagt aaaaatgaat
    13981 gccaaaggac acataataat ccttctgagg ccgtttccgt ctgaatacca gccgtttccc
    14041 agataaaagg actcatagcg gtccatggct tttttcactt cttcaggatt ccatttcttt
    14101 cccacgctct ttagcgccag attgacaatc accttaaagt aatgccagtt attatccgcc
    14161 tgagaataaa gattgatctg atacagccaa tctgccagat gatcctttga tgcctcactt
    14221 aacggctccc acagcttttc cggcaccagt aaaatgccat agctgatagc cgccatttcc
    14281 accatccgct gatccttatt gtgaagatct ccccagtagg aagaagcctc aggatccgtt
    14341 cctgccgcca gtcctttctg gtaaactgaa agaaggcttt cgtcttctcc tccccccggc
    14401 ccagtaggag gccagctccc ataaaaccct ggaaaatcct tccatttctg caattcagtt
    14461 tccatatcct gctgaaaatg ctcccagata aagtccggac ccgtcatcag tataatgacg
    14521 ggtcagaggg cgtatcattt taaggagcat cttctgataa tccgctttct tcttcatatc
    14581 attctccttt tgtatctctt attctgcttt caattcgtct aaaatctcct aatactgctc
    14641 cattaaggac tttgcagctt cctcagtttt ctgttcctgc cggttccccc taatccaaat
    14701 gaaacaccgg cgcaagatca acgcttacag gactattatc ctcgtttgtt tccataatat
    14761 ccgccataaa gtcccaccat tttctgttaa tgggcgtatc ggcggaggcg gcccagagcg
    14821 cttcatcctc gatttcaata tagccgtaaa gcacatgggt ttcccggtct aaatagatgg
    14881 aataattatg gcctccgtga gcgtggatca tttccttcat ttccggccaa agcaggttgt
    14941 gacgcttttc atactcctct tccatgcccg gatataaaaa catcttaaag gcttttcgga
    15001 tcatgcagat gcctccttta tttctcgtat aaaaacttct ccggcagagt tacatgaaaa
    15061 tcctttgcca gatccctgaa attctgagga gtgatggtct gaagcttatc cgggcgcatg
    15121 gacagcatct taaccagaat ctccgcagat ttttctacgg tatgcatcag tccaaaggtg
    15181 atgtcaaagt cctcgcctgc ggcaaaggtt ccatgatgcg cccaaatagc cacatcatac
    15241 tgtttcataa gctcactggt agccaccgcg atatcccggc cgccgggaac catccagggc
    15301 acaacgccga taccggcagg gaatacgacc ggacattccg ttgccatttc ccagagctct
    15361 cttgtaaata cctcatcctt cagaggcagt acaaaggtga gggcaatcac attcaccgta
    15421 tgggcatgat agattacccg gtatgcaccg tttgttgcga ttttctttac ttcatggttc
    15481 atcaggtggg aagggagctc gctggttggc tttccgccgt ttactaaccc ccatacgatg
    15541 cggtaattct ctcctgcctg atctaactcg atcatgcaga tggaatcctc aggcttaata
    15601 attacatttc tgagatactt tccgctgcct gttacaagga aatactctcc gcccagagct
    15661 ggaactgtag ttccgatggg acgccattcc tttggctcta attcttcctt tacgctctct
    15721 acttcctcat cctttacacg gtaggatata tttcctccat ttctctcatg ccatccctgc
    15781 tcccagccgt cgtttgccat gcggataaaa ccctgaacaa attctgcatc taaaaatctc
    15841 attttcaatt tcctccttat tttctctgtg acagtacgtc tttctcatat atttttattt
    15901 tttcaaacca gctctggtca ctttccacac cgcactgacg gcagtattca ttccatacat
    15961 cgcccaatgg atacagcttt aactcctcct gcatcatcat aagctctgtc atgcggttct
    16021 catcctgaag ctcctttaac agagctgccg gcgtaagaag tgcggacagc agtgctttct
    16081 ggaagcttct aaagcccaca gcccatgcag aaatacggtt gatgctggcg tcaaagtaat
    16141 ccagcgcaat ataaacacgg tctaaggcgt tatttcttac gatctccttt gcgatttcct
    16201 ttgtctcatc atcaaaaagc accacatggt cgctgtccca gcgcaccgga cgggtcacgt
    16261 gaagggcaat ctccgggaag aagcaaagaa gggcgggaat cttgtcagag accatctctg
    16321 tagggtgata atgtccgttg tccattaagg gcagtactcc tttatgggtt gctgcaaagg
    16381 aaagggcaaa ttcggcggag cctgccgtat aagattcaac gccgataccg aataccttgg
    16441 actctacaca gggcttaaca agctctggat cgtacggctc tgataaaatc tcctcaatcg
    16501 attccttata gcgcattctg ggacccatcc ggtctgcagg cacatcctta aagccgtcgc
    16561 ctgtccagat attcattacg catggaactc ctgtctcctg agcgaaatac tgggaaatac
    16621 ggatgcaggc ctttccatgg cggatccaga actccctcgt ttccttatca ggactggata
    16681 aggtctgtcc gtcctttacc ttttcatggg agaagaaggt agggttaaaa tcaatgccca
    16741 tattgtgctt tttggcaaat tccacccact tcgcaaaata ctttggctct agctgatccc
    16801 tgtctgcatt ctccccctcc tcaaaaatgg catagcttgc atggaggttt aatttctttt
    16861 tgcccggcat caggctcata gccatctcca tgtcatccat cagctcctga ggcgttctgg
    16921 ctcttcccgg atagtttcct gtggtctgga taccgccggt tagggggccg tcctgatcaa
    16981 agccctttac gtcatctccc tgccagcagt gaagggaaac aggaatgttt tttaaggtct
    17041 ggatcacctg gtctgtgtcg attcccaggc ttttatacat ctcttttgct gattcatagc
    17101 gttctgtctg attcataaca attctccttt cgattgattc tggttatcat tccggcatat
    17161 attcatggat ggaaaatgac gttcccacac acctgcgggc ctgttctaag gtgtcaaatt
    17221 ctcctttttg aatcatctga gccatcagat ttccgatggc cgtggcttct cccggccctg
    17281 caagcacccg gcggccagat ttcttagcag tcaggcggtt taaatactcc gcattggaac
    17341 caccgcccac gatacagatg gtattataat gactgcccgt aaggccttcg a
//
LOCUS       DBMDUH010000042        17175 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_1933_length_17175_cov_4.854106, whole genome shotgun sequence.
ACCESSION   DBMDUH010000042 DBMDUH010000000
VERSION     DBMDUH010000042.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 17175)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 17175)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17175
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1933_length_17175_cov_4.854106"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            162..1637
                     /locus_tag="ACZPCG_05425"
     CDS             162..1637
                     /locus_tag="ACZPCG_05425"
                     /EC_number="3.1.-.-"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HIR1284548.1"
                     /translation="MKHTKKITILHSNDLHGDFLAEQVDEKLVGGVSMLSGYIESVRK
                     EEPNTLYVIAGDMFRGSVIDSEYKGLSTIEIMNALAPDVVTIGNHEVDYGIAHLLFIE
                     KCARFPIINANLYIKNTATRLFTPYKIFRVDGMNILFIGVITQDVINQTKSESLVGAF
                     VDTAAAAAEIGKICNAHNSIDIDFTVILTHIGFEEDKHLARQLDPAWGVDLIIGGHSH
                     TFLEHAVEENGVVIAQAGTGTDQIGRFDIIVDTDGNCIDSYTWRSIPIIADTCPRSPM
                     IEQVLERFTQQIDEKYNFIIGRFRRELTHPERTQETELGNLFADIFTESLGVDIMLIG
                     SGSIRSEKLGPIVTYADLIEGFPYDDGVYMFKVTAGQLRKMLLYMVRDEAFLGHTEFY
                     QIPSKFRFCYNRERGEFDSFTYCGKELGREISDDTVFTVGMQNFHFQNVEKFLSISYE
                     TISQLQKPRCIASSCQDILMEYFREHEWLDSAVDGRMTIEG"
     gene            1843..3294
                     /locus_tag="ACZPCG_05430"
     CDS             1843..3294
                     /locus_tag="ACZPCG_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HIR1284549.1"
                     /translation="MGLIRAGIGAMGGTLADQWKEFFYCDSMGKDVLVTKGQKRTSGR
                     SSNTKGNDNIISNGSGIAVADGQCMLIVEQGKIVEFCAESGEFTYDTSTEPSIFSGNL
                     GDSVKKTFQVIGKRFAYGGDTGKDQRVYYINTKEMGEILYGTATPVPFRVIVNEELGY
                     KLSVDIRCNGNFTYRICDPLLFYTNVCGNVEQDYESSEIAGRLKGELMTALQPALSTL
                     SAKKVQYYEIPAHTQDLSDALNEVLSKTWKEKRGIEVFSFNINSLSIPEDQRKKINEW
                     EENAMTTNPTTAAARLVGGQIDAMKAAAGNSAGAMTGFMGMGMAMNAGGMGGMNAQNL
                     FAMGQQQPQAAPQPQAAPQPAAPEQPQGDTWTCSCGTTVRGKFCPECGSKKPEPKIEP
                     AGGWKCKCGATVTGKFCPECGSPKPADSEGWTCSCGAVNKGKFCQNCGAKKPQGAPLY
                     RCDKCGWEPEDPKNPPKFCPECGDPFDDGDIKN"
     gene            3442..4584
                     /locus_tag="ACZPCG_05435"
     CDS             3442..4584
                     /locus_tag="ACZPCG_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4428 domain-containing protein"
                     /protein_id="HIR1284550.1"
                     /translation="MGFFDKKYCDVCGEKIGLLGNRKLENGNLCKDCAAKLSPFFSER
                     KSSTVEEIKEQLAYREENRKELERFQATRIIGERMKVMVDEAGKRFVVTSSSDLLKAN
                     PDIINLSDVISCNLDLDVEDTEIMRKDSQGKEVSYNPPRFCYSYNFNIEIGVRNPWFT
                     QIRFRLNPSDVEIVDEQSGGGFGGVGRLTSNRRQSMVHPEYNADYRKYQRMSDEIIAV
                     LLNQPMPASVLADQMMDGPGMVNKILQTGAGMLAGGMMQGGNMMQNRNMMQGGNVMQN
                     GNVMQNGNMMQNGNMMQGGNVMQNGNMMQNGNMMQNGNMAQSGNWTCSCGMTNTGRFC
                     QNCGAQKPEAIRYRCDKCGWEPADPAHPPKFCPQCGDPFDGGDRTN"
     gene            4642..5808
                     /locus_tag="ACZPCG_05440"
     CDS             4642..5808
                     /locus_tag="ACZPCG_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284551.1"
                     /translation="MKKSRFMTAALAAMLILSLTACGGEKKPAETKGSVQASQETTAV
                     AAKETEAAAKETTEKETEPLETEAKIPENAEEITMTNQKLQIQAKFYLPKQEGEWEVE
                     NRDGRDPVTPVQKNVYKAKINEDQSVYVTIQLTATTLESLEKQMDGKEKITIRDYPGT
                     FETGTTSWNYLIDFGKYADGLETYMLVKFWAGDDSYAQVAELKETRDLFLDTVTVATD
                     YEGKEDRSGRKYMGTPTCSLPESFEYNGVTAEVTQPADSYCVHQLRSEIHETDEIPVR
                     LVGLMTSTINQATYEVTEGYSDYTIAGYPAHIKQTKFVGYLSTDVRFQVGDNYYRAFA
                     YTYVDDSNMDVKATAEAVKVLNENETVYFQKSLDFLDAMIKAAEFTEPDEEWFK"
     gene            5832..6317
                     /locus_tag="ACZPCG_05445"
     CDS             5832..6317
                     /locus_tag="ACZPCG_05445"
                     /inference="COORDINATES: protein motif:HMM:NF028039.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5067 domain-containing protein"
                     /protein_id="HIR1284552.1"
                     /translation="MKRKQHRWLLLLLVGVMSLGFAACKKASEVPLDPDHNVLDDYEI
                     WYKGFDVTPDADGQSALVLTLDFTNNGKEATSYLWAVSEKVFLDGIGLNTATVLQDGN
                     SKGTVAETQLTEIMPGKTIEVKSAYSLPDGDKKTLEAEFSQLLGKKKGILTIDLEDTL
                     P"
     gene            6372..7466
                     /locus_tag="ACZPCG_05450"
     CDS             6372..7466
                     /locus_tag="ACZPCG_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HIR1284553.1"
                     /translation="MGNIQEYKCPCCGGAIHFDSAIQKMKCPYCDTEFEMEALQGYDE
                     ALNQEKSDEMVWDTSAGTEWGEGETNGLHTYQCQSCGGEIVGDESMAASACPYCGNPI
                     VVTGQFSGALKPDYVIPFKLDKKAAKAGLMRHLRGKRLLPKVFKDQNHIDEIKGIYVP
                     FWLFDTDADADIRYRATKVRSWSDKNYNYTETRFFMVARSGNIGFTRVPVDGSTKMED
                     DLMESIEPFDFKEAVPFQTAYLAGYMADKYDVGAEESIARANERVKRSTEDNFKSTVT
                     GYTSVTTEQSSVRLHDGSAKYALYPVWILNTTWNGKQYRFAMNGQSGKFVGDLPVDKK
                     AAKTWTIGLSAVFAIAAYGVSWLLWLARII"
     gene            7484..8317
                     /locus_tag="ACZPCG_05455"
     CDS             7484..8317
                     /locus_tag="ACZPCG_05455"
                     /inference="COORDINATES: protein motif:HMM:NF016424.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HIR1284554.1"
                     /translation="MKRTGRKLIGMAAAVLLMASAFCTAVYAADGFSSEYERLLDTAY
                     ILSDAEADEITGLLDELSERQKMDVTIATVNNLDGYNSIEACADDLYDYCNFGYGADR
                     DGLMFLVSMEEREWHISTCGYGITAFTDAGIQYVGGQMLDSLADGDYAEAFRIYAACC
                     DDLIDQARAGHPFDEDDVPRKPMSPIVPLVCIVIGAALGIAIVQGMKAQLKSVRKEKS
                     AANYVRPGSMNLTGKQDLFLYSNVNRVKRETKSSSSGGGGSSTHRSSSGTTHGGGGGK
                     F"
     gene            8346..9233
                     /locus_tag="ACZPCG_05460"
     CDS             8346..9233
                     /locus_tag="ACZPCG_05460"
                     /inference="COORDINATES: protein motif:HMM:NF039231.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284555.1"
                     /translation="MKGFDKKRWIAAGIAVIMAAAMPVTAFAENGWTRVSPRSAWDGT
                     YYRSDGQRVTVTDTDKDYVYISFVMNGEPSVDALKFTDGTRTKAETLEVHEIPAIWSL
                     NGDMLYVDASASYSKFFNGTYQKKTDASCWVRQEDGSYIYLDENGNILKNHMTPDGFY
                     VGPDGKWQAQLGMCLITPGRYKSMFQNPDEILYENWSFSMYTSGDTDRPLTGPEDRIE
                     VGCVDYEAYDQKADFLYTKTDLRLFNTMDGYVIEDAGGEVFAWMYPTIHGNDLMVQMY
                     GTEQYHSIRMVEDYSEYGG"
     gene            9301..10782
                     /locus_tag="ACZPCG_05465"
     CDS             9301..10782
                     /locus_tag="ACZPCG_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284556.1"
                     /translation="MTKSLETKRSFLSVSRIVLAVLMCLLFTGCALLDIKDKGGMVNP
                     DAVSEEETGVAENETENDMSRFMKKKTETESDTEITADGDQPVRKPVRETMDGEIQKT
                     VSSGTWMTETGKSALVEDREYVNQLSAAAGVYFIDYTADFTSGGRQEQVKILFPYLEN
                     LPADHRIECDGDEWYVIVPASENWTIAVNRNTWNENTFEDVKGEEIARISDGLPFLLK
                     CNISDLHSNVMLTAQNGNRSASWCPEYNLYAGEVFGNEDFVTLNVMNEFVNTGYLYNM
                     DGSWYCDGNVNDQGQPFFYNVTLQTDLAGNPTSILYYGGLLVDGSEEIRFYWDGTYVA
                     RGPAEYGSDEKTGPWEYEYWLNTPDGERNGTIRMQTENDTLYIQDLSGDSFFPYTGND
                     SATFYYLPQSAMDEAAGEGAGDEGAADDMDYVAHVPEIHEKILQGMSIYEEESEEIYG
                     EPGRVYVLISDNGSQYVREGYYALTTKGVYRMDYLTGEWELIE"
     gene            10798..11295
                     /locus_tag="ACZPCG_05470"
     CDS             10798..11295
                     /locus_tag="ACZPCG_05470"
                     /inference="COORDINATES: protein motif:HMM:NF046575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6935 domain-containing protein"
                     /protein_id="HIR1284557.1"
                     /translation="MKEITLERLPATVEELAAMPQAALTVPEEVAALTVAALARFPEN
                     QEDAVRMLDFLRGPRPLNGIDKQFLKDRFRGKEYLMRSFFAGSSPENNYTPSMPYRVV
                     VSENPYSRSQLAEGYLTLYVNCSGADSPRPLKLRKKPSTGQWFLWEQQLLAGIRIPAA
                     ADPWS"
     gene            11414..12232
                     /locus_tag="ACZPCG_05475"
     CDS             11414..12232
                     /locus_tag="ACZPCG_05475"
                     /inference="COORDINATES: protein motif:HMM:NF019404.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM43 family protein"
                     /protein_id="HIR1284558.1"
                     /translation="MGTIKKIGAIILFVFGGFFAIATIKMIFIDMPKGRKSQNEAVYV
                     GRGAYDPANDGKTVIVCGEMKIVEPAYDDEIGISFDTMRVMRKAQTLKLTDKETKIEA
                     RGYYNKYLEWSSGLSEMKDYTAKVDVGGFHLDQEYMDRIMLDGRAQYDEEKLNQQGYS
                     VVKDRNYSQTFFIQPMDQTKRYPQEGDFRYEYSSSKYGNGSTVTVIGIQDGDTLRFTG
                     SAADCMREGELTKEEFREENRKGGVGSVIMGIILPLAFILGGIFLLISSGVKPE"
     gene            12496..13452
                     /locus_tag="ACZPCG_05480"
     CDS             12496..13452
                     /locus_tag="ACZPCG_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007713621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284559.1"
                     /translation="MLSVPNTTALAATGTKINSVPLSIESEIEAGDTDSDVTVSTSSS
                     KFDIDDVVITNEPSDEWDDGDKPKLKITLYADDDYYFASGFSKSSVSLSGSDGTVTSV
                     SRSSSDTLIVYVTLDALDDDDSDHDLDVSGLEWDESDGTASWEESDDAKKYEVRLYRG
                     SSAVTSVLTTTSTSYDFSSYITKNGYYTFKVRGVYNTSNKGSWEESDSWYVSSSEAQE
                     ISSSGSSNGNTPSNTTSSGTGAWLKDNTGWWYCNADRSYTTNGWQYIDNLWYCFDENG
                     YMKTGWILYKDAWYYCGESGAMLVNTTTPDGYYVNGDGVWVQ"
     gene            13506..15449
                     /locus_tag="ACZPCG_05485"
     CDS             13506..15449
                     /locus_tag="ACZPCG_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-rich domain-containing protein"
                     /protein_id="HIR1284560.1"
                     /translation="MSRYRDRMGNDRKTKREIGRRLRRGSVMMCMMITSAMLLSACGG
                     NSSQSTTAAQATEAAAESGAQDSQNEVMGRVKSVSDTEITVEQGPGGVPSGAPDGQPG
                     QNNDGQQPGGAPDGKGQPPQGSTSDNKNQPSQGSTSDNQNQPPKGGAPEGNGQPPQGE
                     TTTYKITTSTTFTDADGASIQVSDIKEGDFVKITTDSNGTAVQISLADMPGGPDGPQG
                     GPGNGAPGAGGPGGGAQSAPTSYSSVKEFTSDTEESGQSYTSEGTDESAVLVSNGANV
                     TLKDFTVNRTSEDSKGGDSSSFYGVGASVLATDGTVNLSGGTITSDADGAAGAFAYDK
                     GTVNISDTAITTTGNTAGGIHAAGGGTVNAENLTVHTSGESSAAIRSDRGGGTMRVKG
                     GSYTSSGTGSPAVYCTADIEVNDAKLTAENSEAVCIEGLNSLSLTGCDLFGHIQENEQ
                     NDCDWTVILYQSMSGDSEVGNAVFNMTGGSLTSENGGLFYTTNTESTFYLNNVNITPS
                     SNNEFFLKCTGNANKRGWGQSGANGADCSFTAENQKMEGDVVWDSISNLKFKMTEGSI
                     LTGGFIQDESCVGNGGSGTADLSIDAESTWIVTKDSQLSSLTNKGTIKDAEGKTVTIK
                     GSDGTVYVQGDSAYTVTVESYTA"
     gene            complement(15551..16372)
                     /locus_tag="ACZPCG_05490"
     CDS             complement(15551..16372)
                     /locus_tag="ACZPCG_05490"
                     /inference="COORDINATES: protein motif:HMM:NF018604.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HIR1284561.1"
                     /translation="MKQKNGLQMSSSLPVMILLNLSGGTQDACSYFLRDHVFANAQTG
                     NIVLMGCYLISEDLRNSIRYFLPVAFFALGVLMACLIHSRFRHTGKIHWRHLVLTLEI
                     ILLFIVGFIPGQLNWLANGLTSFACAMQVQSFRTVRGSAYASTMCIGNLRSGMDHFYK
                     AITEKRKDELKTAAIYLTAIGSFTIGAACGGHLTNHFSYRTIWISCGLLILCFLLMLI
                     EEEDDLEAVEKKERKELKMEQKELKEEQKELQKEKAQLKEEQHIIQELDEKIQHH"
     gene            complement(16549..16956)
                     /locus_tag="ACZPCG_05495"
     CDS             complement(16549..16956)
                     /locus_tag="ACZPCG_05495"
                     /inference="COORDINATES: protein motif:HMM:NF015567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIR1284562.1"
                     /translation="MRRYIIASHHLLAHGLKDTLNFLTSMDGIIDISAYMDDTDLPTQ
                     IKELFDSFDPEDEVVMMTDMLGGSVNQQFCPYVNDHRHLICGINLPCALSLVLQPQDM
                     PLTAETIRTIVEESRSHLIYVNEYNDGANEDDE"
BASE COUNT         5082 a         3294 c         4616 g         4183 t
ORIGIN      
        1 catcaccaaa tatatcagat atattgaaaa atagagaggt tgatttttta gctgagacag
       61 acagcctgag tatggaacgg attgtatttg aaaattgtct gatggatgat ggggaaaccg
      121 taaatacagt tgatgaacat taaaaaaagg gggatgcgat catgaaacac acaaaaaaaa
      181 ttacgatcct gcattcaaat gacctgcatg gggattttct tgcagagcag gtagatgaaa
      241 agctggttgg tggagtttcg atgctgtccg gttatattga gagtgtccgt aaagaagaac
      301 cgaatacgtt gtatgtgatc gcaggagata tgttccgcgg ttcagtcatc gattcggaat
      361 acaaagggct ttctacgatt gaaatcatga atgctctggc tcctgatgta gtcactatcg
      421 gaaatcatga ggttgattac gggatcgccc accttctgtt tatcgagaaa tgtgcaagat
      481 ttccgatcat caatgcgaat ctttacatta agaatacggc gaccagatta tttaccccgt
      541 ataagatctt ccgggtggac ggtatgaaca tcctgttcat cggtgtcatt acccaggatg
      601 tgatcaatca gacgaaatcg gaatcattag ttggagcgtt tgtggataca gctgctgcgg
      661 ctgctgaaat tggaaagatc tgtaacgcac acaacagtat cgatatcgat tttacggtta
      721 ttctgacgca tattggtttt gaggaggata agcatcttgc aagacagctg gatccggcct
      781 ggggtgtaga tcttattatt ggaggacatt cccacacatt tctggagcat gctgtggaag
      841 aaaacggcgt tgtgatcgca caggctggta ccggtacgga ccagatcggc cgctttgata
      901 tcattgttga caccgacgga aactgtattg attcatatac ctggcggagc ataccgatca
      961 tagcagatac ctgtccgcga agtccgatga tagagcaggt gctggagcgt ttcacacagc
     1021 agatcgatga gaaatataat ttcattattg gccgtttccg ccgtgaactg acacatccgg
     1081 agcggacgca ggaaacagag cttggaaacc tgtttgcaga tatttttacg gaaagtctcg
     1141 gcgtggatat catgctgatc ggttccggca gtatccggag tgagaagctt ggtccgattg
     1201 ttacatatgc agacctgatc gaaggattcc cttatgatga tggtgtatat atgttcaagg
     1261 tgacggcagg ccagctgcga aagatgctgc tctacatggt ccgggatgag gcgtttttag
     1321 gacatacaga gttttaccag atcccatcaa agttccgttt ctgttataac cgtgagcggg
     1381 gcgagtttga ttcctttacc tattgcggca aggaacttgg cagagaaatc agtgatgata
     1441 cggtttttac agttggaatg cagaatttcc atttccaaaa tgtggagaag ttcctgagta
     1501 tatcttatga aactatatcc cagcttcaga aaccacgctg cattgcttcc tcctgtcagg
     1561 atatcctgat ggagtatttc agggaacatg agtggctgga ttcagctgtg gatggccgga
     1621 tgaccataga aggataaact atatatatga aataatttaa aacagacact atatcttgta
     1681 tactcacggt gagtattttg ccaaaattgc cctaaaaggg ataaatatac taacggtgag
     1741 tatatatttt cgtgaaaaat ctgatatatt caaaccagat caaagatttg gatcatgaaa
     1801 aggatcatga aaaatccggt gatatggaaa ggagaaatga gtatgggact tattagagca
     1861 ggaattggag caatgggcgg aacattggca gatcagtgga aagagttttt ctactgtgat
     1921 tccatgggaa aagatgtact ggttacaaag ggtcagaagc gaacatccgg tcgttcctct
     1981 aacacaaaag gtaatgacaa catcatttcc aacggcagtg gaattgctgt tgcggatgga
     2041 cagtgtatgt tgatcgtgga acagggaaag atcgttgagt tctgtgcaga gtctggagaa
     2101 tttacttatg atacttccac agagcccagt attttcagcg gtaatctggg agacagcgtt
     2161 aagaagacct tccaggttat tggaaagcgt ttcgcatatg gtggcgatac aggaaaggat
     2221 cagagagttt attacattaa cacaaaagaa atgggagaga tcctttacgg tacagcaaca
     2281 ccggttccat tccgcgtgat cgtaaatgaa gagcttggat ataagctttc tgtagatatc
     2341 cgttgcaacg gaaactttac ataccggatc tgtgatccgc tgcttttcta tacaaacgta
     2401 tgtggaaacg tggaacagga ttatgagagc tcagagatcg caggacgttt aaagggcgag
     2461 cttatgacag cactgcagcc ggcactttcc acactgtctg caaagaaagt ccagtattat
     2521 gagatcccgg ctcatacaca ggatctgtct gatgcattaa atgaagtcct ttcaaagaca
     2581 tggaaggaaa aacgaggcat tgaggtattt tcctttaata tcaattcctt aagtattccg
     2641 gaagatcagc gtaagaagat caatgagtgg gaagaaaatg caatgaccac caatccgaca
     2701 acagcggctg cccgtcttgt tggaggacag attgatgcga tgaaagcagc tgcaggaaac
     2761 agcgccggcg caatgacagg gtttatggga atgggaatgg caatgaatgc cggcggaatg
     2821 ggcggcatga atgcccagaa cctgtttgca atgggacagc agcagccgca ggcagcaccg
     2881 caaccgcagg cagctccaca gccggcagcg ccggaacagc cacaaggtga tacatggacc
     2941 tgttcctgcg gaacaacggt gcgaggaaaa ttctgtccgg agtgtggttc aaagaagccg
     3001 gagccgaaga tagagccagc aggaggctgg aagtgtaaat gtggtgcaac agtaaccgga
     3061 aagttctgtc cggaatgcgg ttctccgaag ccggcggaca gcgaaggctg gacctgttcc
     3121 tgcggagccg taaataaagg caagttctgt cagaattgtg gagcgaagaa accgcaagga
     3181 gcaccgttat accgctgtga taagtgcgga tgggagccgg aagacccgaa aaatccaccg
     3241 aagttctgtc cggagtgcgg tgaccccttt gatgatggag atatcaaaaa ctaaaaatta
     3301 agaacagtaa catactggcc gcggttgttt cctgaaaggg aatctgaccg cggcacatga
     3361 aaattaaggg aactgtatgt tctcacaaga tcaggtgcag gcaggacttc aagagtacat
     3421 ctgttagaaa gggaggttta tatgggattt tttgataaaa aatactgtga cgtatgtgga
     3481 gaaaagatag gtcttcttgg caaccggaaa ttagaaaatg ggaatctctg taaagattgt
     3541 gcggcgaagc tttccccatt cttttccgaa cggaaatcgt ctaccgtaga ggagatcaag
     3601 gaacagctgg cttaccgtga agaaaacaga aaagagctgg aacgcttcca ggcaacgcgt
     3661 atcattggcg agaggatgaa agttatggtt gacgaagcgg gaaaacgctt tgtagtgacc
     3721 tcttcttcag accttttaaa ggcaaatccg gatattatca acctttctga cgtgatctcc
     3781 tgtaatctgg atcttgatgt agaagataca gaaatcatga ggaaggattc acagggcaag
     3841 gaggtcagtt ataatccgcc gagattctgt tattcctata actttaacat tgagatcgga
     3901 gtaagaaatc catggtttac ccagatccgt ttccgcttaa atcccagtga tgtggagatt
     3961 gttgatgagc agtccggtgg tggttttggt ggagtcggaa gactgacaag caacagacgc
     4021 cagagcatgg tacatccgga gtataatgcg gattaccgga aataccagcg tatgtccgat
     4081 gagatcattg ctgttttatt aaatcagccg atgccggcca gtgtccttgc agatcagatg
     4141 atggacggac cgggaatggt aaataagatt ctgcagacag gtgcaggaat gctggctggc
     4201 ggaatgatgc agggcggaaa catgatgcag aatagaaaca tgatgcaggg cggaaatgtg
     4261 atgcagaatg gaaatgtgat gcagaatgga aatatgatgc agaacggaaa tatgatgcag
     4321 ggcggaaatg tgatgcagaa tggaaatatg atgcagaatg gaaatatgat gcagaacgga
     4381 aatatggcac agagtggaaa ttggacctgc tcctgcggta tgacaaatac cggaagattc
     4441 tgccagaact gcggcgcgca gaagccggag gcgatccggt accgttgtga caaatgcggc
     4501 tgggagccgg cagatccggc acatccaccg aaattctgtc cgcagtgcgg agatcctttt
     4561 gacggtggag acagaactaa ttaagtgatg atgttgttag aattgcgaga gtgcatcccg
     4621 gtggaaaagg aggaacggat aatgaaaaaa agtagattta tgacggctgc tctggcagca
     4681 atgctgatac ttagcctgac tgcatgtggc ggtgaaaaga agccggcgga aaccaaagga
     4741 tccgttcagg catcacagga gactacggct gtggcagcga aagaaacaga agctgcggca
     4801 aaagaaacta cagaaaaaga aacagagcct ttggagacag aggcaaagat accggaaaat
     4861 gctgaagaga tcaccatgac gaaccagaag cttcagatcc aggcaaagtt ctatcttcca
     4921 aaacaggaag gagaatggga agtagaaaac cgggatggaa gagatccggt tacaccggtg
     4981 cagaaaaatg tttataaagc gaagatcaat gaggatcaat cagtgtatgt gacgatccag
     5041 cttacagcaa cgacactgga aagtcttgag aagcagatgg atggcaagga aaaaatcacg
     5101 atccgggatt atccgggaac atttgaaaca ggaactacat cctggaatta tctgatcgat
     5161 ttcggtaaat atgcggatgg attagaaact tatatgctgg tgaaattctg ggccggagat
     5221 gacagctatg cccaggtggc agagctgaag gagacaagag atctgttcct tgatacggtt
     5281 acggtggcaa cagactatga aggcaaggag gaccgcagcg gacgcaaata tatgggaact
     5341 cctacatgtt ctcttccaga aagctttgaa tacaatggag taactgctga agtgacacag
     5401 cctgcggatt cttactgtgt gcatcagctc cgttcagaga tccacgagac agatgagatt
     5461 ccggtcagac ttgttggcct tatgacctct acgatcaatc aagcaaccta tgaagtgaca
     5521 gaaggatatt ccgactacac catagccggt tatcctgcac atatcaaaca gacaaagttt
     5581 gtaggttatc ttagcaccga tgtccgtttt caggtcggag ataactatta tcgtgcattc
     5641 gcatatacct atgtagatga ttctaatatg gatgtgaagg caacggcaga ggctgttaag
     5701 gttctgaacg agaatgaaac tgtatacttc cagaaatcac tggatttcct ggatgccatg
     5761 atcaaagctg cagaatttac ggagccggat gaagaatggt ttaaataaat acaggaagca
     5821 gggggtggaa tatgaagcga aaacagcacc gctggctgct cctgctcctg gttggagtga
     5881 tgtctcttgg atttgcagct tgtaagaagg catcagaggt tcctttggat cccgatcaca
     5941 atgttctgga tgattatgag atctggtata agggttttga cgttacgccg gatgcggatg
     6001 gtcagtcagc cctggtcctg actctggatt ttaccaataa tggcaaagag gcaacctctt
     6061 atctctgggc agtgtcagaa aaggtgtttc ttgatgggat cggccttaac acggcaactg
     6121 tgctgcagga tgggaactcc aagggaacgg tcgctgaaac acagctgact gagatcatgc
     6181 cggggaaaac aattgaagtg aagtctgcat atagtctgcc ggatggtgat aaaaagacgc
     6241 tggaagcaga gttttcacaa ttgttaggaa agaaaaaagg gatcctaacc attgacctgg
     6301 aggatacgct gccgtagaaa ggcagcgccc tttagggatc tggagaaaag aaagggaagg
     6361 agaacaggag tatgggaaat atacaggaat ataagtgtcc gtgctgtggt ggtgccatcc
     6421 actttgacag tgcgatccag aagatgaaat gtccgtactg cgatacggag tttgagatgg
     6481 aggcacttca gggatatgac gaggcactga atcaggaaaa gtcggatgaa atggtatggg
     6541 ataccagtgc aggaacagaa tggggtgagg gagagacaaa tggccttcac acctatcagt
     6601 gccagtcctg cggcggcgag attgttggag atgagagcat ggcagcatct gcgtgtccat
     6661 attgtggaaa ccccatcgtc gtgaccggac agttttcggg agcattaaag ccggattatg
     6721 tgattccatt caagcttgat aaaaaggcgg caaaggccgg tctgatgcgt catctgagag
     6781 gaaaacggct ccttccgaag gtctttaagg atcagaacca tattgatgag atcaagggca
     6841 tttacgttcc tttctggctg tttgatacag atgcagatgc ggatattcgc tatcgtgcga
     6901 caaaggtcag aagctggagc gataaaaact ataattatac ggaaacaagg tttttcatgg
     6961 tagcacgcag tggaaacatt ggctttacac gggttccggt agacggatcg acgaagatgg
     7021 aggacgatct tatggaatcc atcgaacctt ttgattttaa agaggcagtc ccgttccaga
     7081 cagcatatct tgcaggttat atggcagata aatatgatgt tggagcagag gagagtattg
     7141 cccgtgcgaa tgagcgcgta aaacgctcca cagaggataa tttcaaatcg acagtaaccg
     7201 gctatacgtc agtgacaaca gaacagagca gtgtaaggct tcatgacgga agtgcgaaat
     7261 acgcacttta cccggtctgg atcttaaata caacatggaa tggaaaacag taccgtttcg
     7321 ccatgaacgg acaaagtgga aaattcgtag gtgatctgcc agtagataaa aaggcagcaa
     7381 aaacctggac aatagggctt tccgcagtgt ttgcaatagc agcttacggt gtcagctggc
     7441 ttttatggct ggcacggatc atatagaagg gggagtggag aagatgaaaa gaacaggacg
     7501 taaactgatc ggaatggcag ctgccgtgct tctcatggct tccgctttct gtactgctgt
     7561 ttatgcagcg gacggttttt caagtgaata tgaacggctt ttagatactg catatattct
     7621 ttctgatgcg gaagcagatg agatcaccgg gctgctggat gaactcagtg aacgccagaa
     7681 gatggatgta acgatcgcta ccgtaaataa tctggatggt tacaacagca ttgaagcctg
     7741 tgcggatgat ctttatgatt attgtaattt cggctacggc gcagaccggg atggtctgat
     7801 gttcctggtc agcatggagg aacgggaatg gcatatctcc acctgtggat acggaatcac
     7861 ggcatttacg gatgccggta tccagtatgt gggaggccag atgctggatt cccttgcaga
     7921 tggtgattat gcagaggcat ttcgcatcta tgcagcgtgt tgtgatgatc tgatcgatca
     7981 ggcgcgtgca ggccatcctt ttgacgagga tgatgttccg agaaaaccta tgtctccgat
     8041 agtacccctt gtctgtatcg taattggtgc tgctcttgga atcgcaattg tccagggcat
     8101 gaaggcacag ctgaagagtg tacgaaaaga gaaatcagca gccaattatg tacggccggg
     8161 aagcatgaat ctcacaggaa aacaggatct gttcctgtac agcaatgtta accgggtgaa
     8221 aagggagaca aagagcagca gcagcggtgg tggcggaagc tcaacccata ggtcttcttc
     8281 tggaaccaca catggcggtg gcggcggaaa attttaaaag gatcaatacg gagaggagga
     8341 tggaaatgaa aggttttgat aaaaagagat ggattgcagc aggaattgct gttattatgg
     8401 cagctgcgat gccggtcacg gcgtttgcgg agaacggatg gacacgcgta tcgccacggt
     8461 cggcatggga cggaacctac tatcgttctg acggacagcg tgttacagtg acagacacgg
     8521 ataaggacta tgtatatatt tcctttgtca tgaatggtga gccatctgtg gatgccctga
     8581 aatttacgga cgggacacgg acaaaggcgg aaacactgga ggttcatgag attccggcaa
     8641 tctggtcatt aaatggggat atgctttatg tggatgcatc tgcatcctat agtaaattct
     8701 ttaacggtac ttaccagaaa aagacagatg catcctgctg ggtaagacag gaggacggaa
     8761 gctacatcta tctggatgag aatggaaata tcttaaaaaa tcacatgacg ccggatggtt
     8821 tttatgtagg accggatgga aaatggcagg cacagctggg aatgtgcctg atcacaccgg
     8881 ggcgttataa gtcaatgttc cagaatccag atgagatact ctatgaaaac tggagcttca
     8941 gcatgtatac ctctggtgat acggatcgtc ctctgacggg accggaagac cggatcgagg
     9001 tagggtgtgt agattatgag gcatatgatc agaaagcaga tttcctgtat acaaagacgg
     9061 atctccggct gtttaatacg atggatggct atgtgatcga ggatgcagga ggagaagtat
     9121 ttgcatggat gtatccgacg atccacggaa atgacctgat ggttcagatg tatggaacgg
     9181 agcagtatca ctccatccgc atggttgagg attattcaga gtatggcggc tgatgcgtga
     9241 tggatcatat ggggataacc tggaagaaag tgcgaagtgc atagaaggag ggagaggagc
     9301 atgacaaaat cgttagaaac gaaaagatca tttttgagtg tcagccggat cgttctggcg
     9361 gtcctgatgt gcttgctgtt taccggctgt gcgctcctgg atataaagga taagggcgga
     9421 atggtgaatc cagatgcagt atccgaggaa gaaacaggcg tggcagaaaa cgaaacagag
     9481 aacgatatga gccggtttat gaaaaagaag acagagacgg agtctgacac agagatcact
     9541 gcagatggcg atcagccggt ccgtaagccg gtccgtgaga caatggatgg ggaaatccaa
     9601 aaaacggttt ccagtggcac ttggatgacg gagacaggga agagtgcact tgttgaggac
     9661 cgggaatatg tgaatcagtt gtctgctgcg gcgggtgtgt attttatcga ctatacggca
     9721 gattttacaa gtggaggaag acaggaacag gttaagatcc ttttcccata tctggagaat
     9781 cttccggcgg atcaccggat tgaatgcgac ggagatgagt ggtatgttat tgttccggca
     9841 agtgagaact ggacgatcgc ggtcaacagg aatacatgga atgagaatac atttgaggat
     9901 gtaaagggtg aggaaattgc ccgtatatcg gatggacttc ccttcctttt aaagtgtaat
     9961 ataagtgatc tgcattcgaa tgtgatgctt acagctcaga atgggaacag atcggcgagc
    10021 tggtgtccgg agtacaatct gtatgcagga gaagttttcg gaaacgagga ttttgttacg
    10081 ctgaatgtta tgaatgagtt tgtgaatacc ggatatcttt acaacatgga cggaagctgg
    10141 tattgtgatg ggaatgtaaa tgatcaggga cagccatttt tctataatgt tacactccag
    10201 acggatctgg ccggaaatcc aacttctatc ttgtattatg gaggcctttt ggtagatggc
    10261 agcgaggaaa tacgtttcta ctgggacgga acctatgtag cacgcggacc ggctgagtat
    10321 ggatccgatg agaagaccgg tccgtgggag tatgagtact ggctcaatac gccggatgga
    10381 gagcggaatg gtacgatccg gatgcagacg gagaatgata ctttgtacat ccaggatctg
    10441 agcggtgatt ctttcttccc gtataccggc aatgattctg ccaccttcta ttacttacca
    10501 cagagcgcaa tggatgaagc agcaggtgaa ggcgccggtg atgaaggagc tgcagatgac
    10561 atggattacg ttgcgcatgt gccggagatt catgagaaga tcttacaggg tatgagtatt
    10621 tatgaggagg aatcagagga gatttacgga gagcctggaa gagtctatgt gctgatcagc
    10681 gacaatggaa gccagtatgt ccgggaaggc tactatgcat taaccactaa aggggtatac
    10741 cgtatggatt atctgactgg agagtgggaa ttaattgaat gaagaggggg aaatcttatg
    10801 aaggagatca cattagaacg cttaccagca acagtagagg aacttgcggc aatgcctcag
    10861 gcggcgctta ctgtcccgga agaggttgcg gcacttacgg ttgcagcact ggcgagattt
    10921 ccggaaaatc aggaggatgc agtccggatg ctggatttct taaggggacc aagaccgtta
    10981 aatgggatcg ataaacagtt tctaaaagat cggttccggg gaaaagagta tctgatgcgg
    11041 tcattttttg caggatcttc cccagagaat aactatacgc cgtcaatgcc atatcgtgta
    11101 gttgtatcag agaatccata cagcagaagc cagcttgcgg aaggatatct gacactttat
    11161 gtaaactgca gcggcgcaga cagtccgaga ccattaaaac tccgtaaaaa accatctacg
    11221 ggacagtggt ttttgtggga acagcagctt cttgccggga tacggatccc tgcagcagca
    11281 gatccgtggt cataaaattc caggcgtata gcacgatgtc ctcccggagt ctcttatgca
    11341 cctgcttttg tggctgagct tcaggtacaa aaagattttg ggggaatttc actggtaaaa
    11401 ggagggagag tctatgggta ccataaaaaa gatcggtgcc ataatcctgt ttgtgtttgg
    11461 cggctttttt gcgatcgcta cgatcaaaat gatctttatc gatatgccga agggaagaaa
    11521 atcccagaat gaggctgtat atgtagggcg cggggcgtat gatccggcaa atgacggaaa
    11581 aacagtcatt gtctgcggtg agatgaaaat cgtggaaccg gcatatgatg atgagatcgg
    11641 gatttctttt gatactatgc gtgttatgag aaaggcgcag accttgaagc taacggataa
    11701 agagacaaaa atagaggcga gggggtatta taataaatat ctggagtgga gctcaggact
    11761 ctctgaaatg aaggattata ctgcaaaggt agatgtgggt ggattccatt tggatcagga
    11821 gtacatggac cggatcatgc ttgatgggcg tgcacagtat gatgaggaga agctgaacca
    11881 gcagggatat agtgtggtaa aggaccgaaa ctattcacag accttcttta tccagccgat
    11941 ggatcagaca aagcgttatc cgcaggaggg cgatttccgt tacgaatact caagctccaa
    12001 atatgggaat ggatccacag ttactgtgat cgggatccag gatggagata ccttaagatt
    12061 cacaggcagt gcagccgatt gtatgaggga aggagagctt acaaaagaag aattcaggga
    12121 agaaaaccga aaaggcggtg tgggaagtgt tattatgggg atcatactcc cgctggcctt
    12181 tatccttgga ggaatcttcc ttttgatatc atcgggcgta aaaccggaat aacaacagcc
    12241 attaaaaaag tgctgggcat ggattttaat ccatagccca gcactttttt tctcacattt
    12301 ttttcacaca atcaccaaaa tctggtcaaa aaatcccgtt atagtttaag tcaatgaaag
    12361 acaagtaaga gaaaagataa gagaaaaata aaaaagctaa atgcccgtat atatttgata
    12421 cgggttgaaa ggagcaattc tatgaaactt aagggtaaaa taaaagaact tactgcactt
    12481 acttctgcga tctgcatgct gtctgtaccg aatacaacgg ctctggctgc tactggaaca
    12541 aaaataaact ctgtccctct ttctattgaa tcagaaatcg aggctgggga tacagatagc
    12601 gatgtaaccg tttccacatc ctcttctaaa tttgatatag atgatgtggt gatcaccaat
    12661 gagccaagcg acgagtggga tgatggcgac aaaccgaaat taaagatcac tttatatgca
    12721 gatgatgatt attattttgc ttccggattt tcaaaaagca gtgtcagctt aagcggttct
    12781 gacggtacag ttacatcagt cagcagaagc tccagtgata ctttgatcgt atatgtaaca
    12841 ctggatgccc tggatgatga tgacagtgat catgatctgg atgtaagcgg gctggaatgg
    12901 gatgagtcag acgggacggc ttcctgggaa gaaagtgatg atgcaaaaaa gtatgaagta
    12961 cggctttacc ggggaagcag tgcagtgacc agcgtgctga cgactaccag caccagttat
    13021 gatttttcaa gctacataac taagaacggt tattatacat ttaaagtaag aggcgtgtac
    13081 aacacttcta ataagggaag ctgggaagag tctgactcat ggtatgtttc ttcaagcgaa
    13141 gcacaggaga tcagttcttc aggttcttca aatggaaata caccttccaa tacgacatct
    13201 tccggtactg gtgcgtggct gaaagacaat acaggctggt ggtactgcaa cgcagaccgc
    13261 agctatacca caaacggctg gcagtatatt gacaatctct ggtactgttt tgatgaaaac
    13321 ggttacatga aaactggctg gatcttatat aaagatgcat ggtattactg tggtgaaagc
    13381 ggtgctatgt tagtgaatac aactacacca gatggttatt atgtaaatgg tgacggtgtc
    13441 tgggtacagt aagagaaaat agatagaaaa tgattttaaa agatgaaagt tgaggcaatg
    13501 cgattatgag cagatataga gatagaatgg gaaatgacag aaagaccaaa agagagattg
    13561 gaagacggct gcgcaggggc agtgtgatga tgtgcatgat gatcaccagt gctatgttgc
    13621 tgtcagcctg tggaggaaat agcagccaga gtactacggc agcacaggct acagaggcag
    13681 cagcagaaag cggcgcccag gacagccaga atgaggtaat gggacgtgtg aagtcggttt
    13741 ctgatacaga gatcacagtt gagcagggac ctggcggagt accgagtggg gcgccagatg
    13801 gtcagccagg tcagaacaat gatggtcagc agccaggagg tgccccagac ggaaaaggcc
    13861 agccacccca gggcagtacc tcagacaaca aaaaccagcc atcacagggc agtacctcag
    13921 ataaccagaa ccagccacca aaaggcggtg ccccagaggg gaacggccag ccgccacagg
    13981 gcgaaaccac aacctataag atcacaacat ccacaacctt caccgatgca gacggtgcat
    14041 ccatccaggt ttctgatatc aaggaaggtg actttgttaa gattactaca gacagcaatg
    14101 gaaccgcagt acagatatct ttagctgata tgccaggcgg tccggatggt cctcagggtg
    14161 gccctggaaa tggggcaccg ggagctggcg gtcctggagg cggggcacag tccgcaccca
    14221 catcttattc ctctgtaaag gaatttacca gtgatacaga agagtccgga cagagctata
    14281 cctcagaagg cacagatgaa agcgcagttc ttgtatctaa cggagctaat gttactctaa
    14341 aagattttac cgtaaacaga acatccgaag acagcaaagg cggtgacagc tccagctttt
    14401 acggagtagg cgcatcggtt cttgccacag acggaactgt taatctaagc ggcggaacca
    14461 ttacttcgga tgcagatggc gctgcaggtg cgtttgccta tgacaaaggc actgtaaata
    14521 tctctgatac tgccattacc acaactggaa atacagcagg cggtatccac gcagcaggcg
    14581 gcggtacagt caatgcagaa aacctgacgg tacatacttc tggtgagtct tccgcggcga
    14641 tccgaagcga tagaggcggc ggcaccatgc gtgtaaaggg cggcagttat acttcttcag
    14701 gaactggctc accggcagtt tactgtacag cagatattga ggtgaacgac gcaaagctga
    14761 cagcagaaaa ttctgaagca gtgtgcattg aaggcttaaa ttccctgtca ctgacaggct
    14821 gtgacctgtt cggtcatatc caggaaaatg agcaaaatga ctgtgactgg acagtgatct
    14881 tgtaccagag catgtccggt gattctgaag taggaaatgc tgtatttaat atgaccggcg
    14941 gcagcctgac ttctgaaaat ggcggtctgt tttacactac aaatacagaa agcacttttt
    15001 atttaaacaa tgtaaatatt accccgtctt ctaataatga atttttctta aaatgtacgg
    15061 gaaatgctaa taagagaggc tggggacaga gcggtgcaaa cggagcggat tgttccttta
    15121 cagcagaaaa ccagaaaatg gaaggagatg tggtttggga cagtatcagc aacctgaaat
    15181 ttaagatgac agagggaagc atccttacag gcggcttcat ccaggatgag tcctgcgtcg
    15241 gaaatggagg cagcggaacg gcagatctgt ccattgatgc cgaatccaca tggatcgtca
    15301 caaaagacag tcagttatct tcactgacca ataagggaac cattaaagac gcagaaggca
    15361 aaactgtgac tattaaggga agcgacggca ctgtttatgt acagggagac agtgcttata
    15421 cagtaacggt agagtcttat actgcatagt aaatggatgc gtttacaatg gtagtactat
    15481 gaattgaata ggaaataaaa aacaggcact gcgaggaaaa taagatcttc gcagtgcctg
    15541 ttctatgtac ttagtgatgc tgtatttttt catccagttc ctgaatgata tgctgttctt
    15601 ctttcagttg agctttttct ttctgaagct ctttctgctc ttcttttaat tctttctgtt
    15661 ccattttcag ctctttgcgt tccttttttt ccacggcttc cagatcgtcc tcttcttcga
    15721 tcagcatcag taaaaagcag aggatcagaa gtccgcagga gatccagatg gtgcggtagg
    15781 agaaatgatt ggtaagatga ccaccgcagg cggcaccaat ggtaaaggaa ccgatcgcag
    15841 taagataaat ggcagctgtt ttcagctcat cttttcgttt ctcggtaata gctttataaa
    15901 aatggtccat gccacttcgc agatttccga tacacatggt gctggcataa gcactgccgc
    15961 gtacagtacg gaaggactga acctgcatag cgcaggcaaa ggaagtaagt ccatttgcaa
    16021 gccagtttaa ctgtcccggg ataaaaccga cgataaacag taggataatc tccagagtca
    16081 ggaccagatg gcgccagtgg atctttcctg tgtgacggaa ccgggagtgg atcaggcagg
    16141 ccattaatac acccagtgcg aaaaaagcaa cgggaaggaa ataacggatg gaatttcgta
    16201 gatcctcgga gatcaggtag catcccatta aaacaatatt tcctgtctgt gcattggcaa
    16261 agacgtgatc acgtaaaaaa taggaacagg catcctgtgt tccgccggat aagttaagaa
    16321 gtatcataac aggcagagaa ctggacattt gcagtccgtt tttttgtttc atgtatatcc
    16381 cttctttcat tccctgtttt atcatttttt tcaaataaaa tcaatttgcg tttttcacac
    16441 ttctgacttt atatattcct gctttttcta cgaaaaaagc agctgttgga aaaaaatggt
    16501 cagctgcttc ttttatgtct gttcaatgaa gtggattttc acgatttttt actcgtcatc
    16561 ctcattagcc ccatcattat actcatttac atagatcaga tggcttctgg actcttctac
    16621 aatagtccgg atggtctctg cagtaagagg catatcctga ggctgtaata ccaggcttaa
    16681 ggcacatgga agattaatac cgcagatcag gtgacgatga tcgtttacat atgggcagaa
    16741 ctgctggttt acactgccac ctaacatatc agtcatcatg actacttcat cctccgggtc
    16801 aaaggaatca aataattctt tgatctgggt tggaagatcg gtgtcatcca tataggcact
    16861 gatatcaatg atgccatcca tggaagtaag aaaatttaat gtgtctttca ggccgtgggc
    16921 cagtaaatga tgggaggcga taatatatct tctcataacg cttctcctta tagttttata
    16981 gttatagtta tatttataat agtgtggcgg ggatcagatg atgttggggt caaaaggtat
    17041 tttgccccct ctgataccga attgctacgt gctttgcact ctcgcaattc taaaaacatt
    17101 cgtaatccgc tcatttttct tcaaaaaatg gcttcttact catgtttttg gcgggtccca
    17161 agttcaaaaa tcctc
//
LOCUS       DBMDUH010000043        16967 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_1954_length_16967_cov_6.931934, whole genome shotgun sequence.
ACCESSION   DBMDUH010000043 DBMDUH010000000
VERSION     DBMDUH010000043.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 16967)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 16967)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16967
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1954_length_16967_cov_6.931934"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>1558
                     /gene="leuA"
                     /locus_tag="ACZPCG_05500"
     CDS             <1..>1558
                     /gene="leuA"
                     /locus_tag="ACZPCG_05500"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HIR1284563.1"
                     /translation="LRDGNQALVEPMVVEEKVEMFNLLVKLGFKEIEVGFPAASQIEY
                     DFLRTLVERRLIPDDVEVQVLVQCREHLIRRTFEALDGVKKAIVHIYNSTSTLQRNVV
                     FHKDMEEIKDIAIVGTKLVQRYAADCDTKIRLEYSPESFTGTELEFALDICTAVQETW
                     GATKENPIIINLPSTVEMTTPNVYADQIEWMNTHFKNRESIILSVHPHNDRGEGVAST
                     ELAMLAGADRVEGTLFGNGERTGNVDILTVAYNMFSQGINPELNIENIREIAEICERC
                     TKMDIPPRHPYAGKLVFTAFSGSHQDAINKGMQALRERNSKYWEVPYLPIDPSDIGRE
                     YEPIVRINSQSGKGGVAFVMDTFYGFKLPKGMHKEFADVIQKISEKQGEVAPEQIMDE
                     FKKEYLERKEPIHFRKCRITDTETADGEFATLAKVVYTDHGMEKHFDGIGNGPIDAVQ
                     RGLEDALGIHIKVLDYTEHAMASGSGAQAASYIHLVDQTTGKATYGVGISSNITRASI
                     RGIFSAVNRLF"
     assembly_gap    1559..1658
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1780..2370
                     /locus_tag="ACZPCG_05505"
     CDS             1780..2370
                     /locus_tag="ACZPCG_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HIR1284564.1"
                     /translation="MQEIHLEGRQRIKSQEMAFTGLFAALIAAGAYMRIDIPVQPFPM
                     HFTLQFFFVLLAGYVLGARRGAACVCVYLAVGLAGIPVFAAGGGPAYLIRPTFGFLIG
                     FVAAAWITGKLCWKRPGGGVAWRLFSAFWGMMAYYLCGMVYFYVISNYVIHMPVSWKL
                     VVINCFLITAAEDFILCVMAAVVARRLMPFALGQYR"
     gene            2384..3196
                     /locus_tag="ACZPCG_05510"
     CDS             2384..3196
                     /locus_tag="ACZPCG_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HIR1284565.1"
                     /translation="MKNKITHVTWTERVWPERGLNGAALKWIAMCSMFIDHIGAVVLE
                     YGLYYQGGAERMDFLLEQSWGVFLYPLSLLLRILGRPAFLIYCFLLVEGFVHTRDRKK
                     YGRNLLLFGLISEIPFNLAVANRVFDPMYQNVFFELFIGFLILCWIERAEKRTYGPKE
                     MDLVCLYTFIGCGAAVLAGADYNAVGILIIVFLYRFRNSRRKQTVAAGVLGFIETLFP
                     TLGAGALAAVPIWFYNGKRGKQQAKFLFYWFYPLHLFVLFLIRWYFLGIPLA"
     gene            3283..4380
                     /gene="glf"
                     /locus_tag="ACZPCG_05515"
     CDS             3283..4380
                     /gene="glf"
                     /locus_tag="ACZPCG_05515"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009273 - peptidoglycan-based cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HIR1284566.1"
                     /translation="MKKYDYILVGSGLYAGVFAWYAKKQGKTCLVLEKRDHIGGNIYC
                     EDVEDIHVHKYGAHIFHTSNKKVWEFVNSLAEFNRYTNSPVANYKGEMYNMPFNMNTF
                     SKMWNISTPAQAKAIIEEQKKEIQGEPKNLEEQAISLVGREIYEKLVKGYTEKQWGRD
                     CKELPSFIIRRLPVRYTYDNNYFNDLYQGIPIGGYNVMIHKLFEGCEIETGVDYLENR
                     EYYNSLGGTVVYTGTIDGFYDYCFGRLEYRSLRFETEVLEEENHQGVAVVNYTDRETP
                     YTRIIEHKHFEFGTQPKTVITREYPVSWQEGMEPYYPVNDEKNQALYQKYAKLAEKEE
                     NVIFGGRLGEYKYYDMDKVIESAMKKAEEIF"
     gene            4398..>4937
                     /locus_tag="ACZPCG_05520"
     CDS             4398..>4937
                     /locus_tag="ACZPCG_05520"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR1284567.1"
                     /translation="MDELRQLVEAYVPYNEQEERDRELILEQIDSGRDILTRNNKNAH
                     ITVSGWVVSPDRKKVLMAYHNIYRSWAWLGGHADGDEDVRRVIKKEIQEESGLTEVKF
                     LSDELFSLEILTVDGHEKRGEYVSSHLHLNVTFLLEGDTEAPLAVKPDENSRVGWIPA
                     DEIESRCSEPWFAERIYSKL"
     assembly_gap    4938..5037
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5038..6838)
                     /locus_tag="ACZPCG_05525"
     CDS             complement(<5038..6838)
                     /locus_tag="ACZPCG_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013279939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR1284568.1"
                     /translation="MQAMILAAGMGKRLKQLTQNNTKCMVKVNGVPMINRTLSQLERC
                     SLSRIVIVVGYEGQKLMEHIRTLDIKTPVVFVDNPIYNKTNNIYSLFLAKDYLLEEDT
                     LLLESDVIFEDSVLDCLVNDSRDSLALVAKYESWMDGTCVRLSEQDDIVDMISKKQFR
                     FSEIEDYYKTVNLYKFSREFSRTHYVPFLEAYTKALGNNEYYEQVLKVLVALDDPGIK
                     AKRLSPDQKWYEIDDIQDLDIAESMFAPEKERTALISRRFGGYWRYPGMLDFCYLVNS
                     YYPPKKMLDEIRSNFDVLIRQYPSGMYVNSLLAARNFSLRQDQILVGNGAAELIKALI
                     GHTTGKLGVIRPTFEEYANRYESDKVVPYYPDNDNFSYTADDLINFFEDKDISCLTVV
                     NPDNPSGNYIPREDMLRLIRWTGERNIHMIADESFVDFADEENATLLQEELLDEYPHL
                     TVIKSISKSYGIPGLRLGIAASGNKDRICLLKKEVAIWNINSLGEFYMQIEEKYRNDY
                     AAALVKVKAERERFAKALGEIPGIRVIPSQADYLMIELTGGHSAGQVTERLLNQYHCL
                     VKDLSSKLSADNRQFLRLAVRNQEEDDVLVDALR"
     gene            complement(6857..7867)
                     /locus_tag="ACZPCG_05530"
     CDS             complement(6857..7867)
                     /locus_tag="ACZPCG_05530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR1284569.1"
                     /translation="MTYNQNHNRIGRSWKEEPLKFGVIIVTYNRLALLKECVSCVLSQ
                     TEPFSNICIVDNHSSDGTADWLKNLPASFQAETDSCTGPFFDICRLKENLGGAGGFAY
                     GMKRLSATDCDWILIIDDDAMIAPDYIAELKKAILSHEYLAYSGTVTTKGAIDTSHRR
                     FLKCPTFMFYEPVPEASYGTPSFEYDISTFCGLLVKASLVREIGLPKTEYFIWFDDTE
                     YCMRFHSRSRILNVNTAVLNHRTAPPGDAPTVSWKNYYGFRNAIDIGKTYSRHPFLYM
                     AYIILNHLAHIVLDSLLALLGNQPAMRRYRAGIYKAVLKGLRQTPNGICKDYLPGSGK
                     RI"
     gene            8025..9509
                     /locus_tag="ACZPCG_05535"
     CDS             8025..9509
                     /locus_tag="ACZPCG_05535"
                     /inference="COORDINATES: protein motif:HMM:NF014051.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HIR1284570.1"
                     /translation="MNKNKSGRTHRMPSIRKNYTYNLIYQVMTLLTPFITTPYLSRVL
                     GPDGTGIQSYTNSVVQYFAIAAALGTASYGQREIAMHRDDREALNRLFWEIELLCAGM
                     TAVCLVFWLGVIGCSKEYAPYYAALSMTLIAVAFDISWFFGGLEEYGLIVLRNMAVKC
                     VGIVLLFLFIRTKNDLLLYVALTAATGLLGNISMWGYLKPFVRKPDIRQLKPLRHLKE
                     TLVYFIPTIATSVYTILDKTMLGWFTDGDKSQNGYYEYATGFVNMSKILIMSFNAVMS
                     ARMSYLFGAGKLDEVRERLRGSLDFVLLLAIPITLGLSGIAAGFVPWFLGDAYLPVIP
                     LMYVCAPLVLVVGLSDCLGSQILTPAGLRAQSGRAIVGGAVVNFILNLILIPKFQSYG
                     AAAASVAAECFITLLYLRLGKGFVTLPLLWKYSWRRLIAGVCMFISVAVLGRTLVKLW
                     GYSPAVTFLQIGAGAVVYGMILLVLRDPFIWKQVERIIKKQEHV"
     gene            9511..10407
                     /locus_tag="ACZPCG_05540"
     CDS             9511..10407
                     /locus_tag="ACZPCG_05540"
                     /inference="COORDINATES: protein motif:HMM:NF016852.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HIR1284571.1"
                     /translation="METAEFYKEEIRCGWRVDEKTKRIWAVQLEMLDEVERICKKYGL
                     KYFADSGTLIGAVRHQGYIPWDDDIDLAMLREDYETFLKVAPGELKEHLVLQTPYREK
                     NYLRAHAQLRNSRTTGCSREDRKAGYNCGIFIDIFPLDAMPEGRVLSRIWAFSIQTLW
                     TMLYTWYRFDYYENATVFGRILNRLGAFLSIPMERAVSFYEAVCRRFNGTNATRVCDT
                     VFIRHLEKNTWKRQWFDEMVYLPFENRRIPAPAGYDGRLKQEYGDYMKPAKAPTMHGS
                     LFLDPDVSYEEYQKNNWRGAEE"
     gene            complement(10489..12000)
                     /locus_tag="ACZPCG_05545"
     CDS             complement(10489..12000)
                     /locus_tag="ACZPCG_05545"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HIR1284572.1"
                     /translation="MRRSLRYMALGVLTASLLSGNALTALADQDVNHGPGVSQAPEVS
                     QEAPGVSGDTTQNGPGIIVEPGSGTGGQESSQSETASPETETPAPEAGTEGQASGETA
                     GQAQEVPPEESTQVPSNQAFLQVQLLRPDMTWTDPIRDDTTIYPGEGGFISMSIYANN
                     LPGDVLYRTYSAPRGWSNWAMNGGHTSWAADCPIEAIQIRLNGVFGNSFDVYYCATLS
                     DGTVCDWSKNGGTNGAMACGRYITGLRFSLWGKGTEGAVYKMDAPLVSAAPDGIQFVN
                     GTPVFSNGTGDLFTGWVWNDRDRYYVVDNAIVTGWQYIDGYKYYFEADGKLVQDLEPY
                     LNAQGPFMLKINKQMNCTTVYIQDGTNGFIIPYKTFLCSTGDDTPLGDHKTPEKYRWR
                     LMNTDEYCQYCTRLDQGIPILLHSVIYERPDPYTLKPVTYNYLGATPSHGCIRYTTED
                     AKWIYDHCALGTTVNVYNSDVPGPFDRPAIQKMIPETQTYDPTDVNVPENGLR"
     gene            12146..13156
                     /locus_tag="ACZPCG_05550"
     CDS             12146..13156
                     /locus_tag="ACZPCG_05550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006778494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HIR1284573.1"
                     /translation="MAETDSRINIIYASNDGYAGHLAASLCSLLDHNRRAEHIDIYVL
                     SVGMCTEYQNRLKSLAASFGRSLSVVELGNLKERFPYKIDTRGFDISAMGRLFAPKVL
                     PESVERALYLDCDTIVCGSIEPLYETELGECLAGMVMEPTVYQEMKETIGMKPEDAYF
                     NSGVILMDLARWRAEDVLKKLLDFYGAHAGSLFACDQDTINGALRGRICSLPPKYNFF
                     TNYRYFRYQTLKKLCAAYEEVGEQAFYEAKRAPVVIHYLGDERPWIRGNHNHYKKYYK
                     KYLTRTPWKDMPLTEGKFLYMQLWWCFNQMTRLCPALRLAISRRLGMRVIDARKTGKE
                     RK"
     gene            13164..14123
                     /locus_tag="ACZPCG_05555"
     CDS             13164..14123
                     /locus_tag="ACZPCG_05555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR1284574.1"
                     /translation="MDSDTDRDKICCIILNYNDAETAAGLAEELKESRLIDDILLVDN
                     CSTDDSWEKFKKLELGWKKEEKEDKEDILPSLWLIRTDGNGGYGSGNQAGIDYAMEYL
                     EPDYIIIANPDVHFFDDCVKRLKEVLEAREDGAIASAMVTDPEGRELFSYWDLQPMWK
                     DLLDTAPLARRMFAPVLKTPLLELPEGGDGNTRIVGALPGSFFMIKTGCFTPGDLEEL
                     FDRNVFLYYEEKILGQKLKNLGLKALLAVDARYVHAHSVTIDKCYKKAADRQRLLHES
                     KLYYYKHYLHKGPTSLTAAGMFLGLVLAEVSVLTGLLRIKAGR"
     gene            14151..14861
                     /locus_tag="ACZPCG_05560"
     CDS             14151..14861
                     /locus_tag="ACZPCG_05560"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007887010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1284575.1"
                     /translation="MDKVLLVIPAYNEEKNIERVVDQLTESFPELDYIVVNDGSKDRT
                     AEICRKRGYRLLDLPVNLGLAGCFQAGMKYAWEKGYRYAIQFDGDGQHRPEFIKTMRE
                     KMDEGYDIVIGSRFVTEKKDWSFRMLGSRIIGAAIRLTTGAHIKDPTSGMRLFNRKMI
                     GEFALNLNYGPEPDTISFLIHQGARVAEVQVTIDDRTAGESYLKPVTAVKYMVRMLLS
                     ILVIQNFRKRQQDAKTKG"
     gene            14868..16163
                     /locus_tag="ACZPCG_05565"
     CDS             14868..16163
                     /locus_tag="ACZPCG_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140327 - flippase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HIR1284576.1"
                     /translation="MKRMTQALLYKGDHINWANVVWNIAGSFVYAFASMVLSFLVIRL
                     AGEEQGGIFSFGFSTLGQQLFLVAYFGIRPFHITDGAGEYTFGDYREHRNITCLIALA
                     AGAVFMMIRHGAYLRGVGDYNGEKCTILLLLVVYKVIDGYADVYESEFQRQGSLYLTG
                     KSNFFRTILSVSVFTVILAAFENLLLACLAAVAAQIGGVILFNLDVIHALSAVKWEAK
                     KGQCRKLFLSTGFLFVSVFLDFYIFSSAKYAIDARMNNAASGYFNLIFMPTSVIYMVA
                     NFVIRPYLTVLTEFWGKDDRRAFFNTLKRISAVIAGLTVLAVGGTLCLGKWVLSVMEQ
                     IMGGDPGVLTAHFWAFTGIVLGGGFYAFANLMYYALVIMRRQKTVFFVYGAGALAAVV
                     LSGALVGAFGIGGGALCYLLLMAGLCAGFGICTAIGLKK"
     gene            16184..>16967
                     /locus_tag="ACZPCG_05570"
     CDS             16184..>16967
                     /locus_tag="ACZPCG_05570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR1284577.1"
                     /translation="MEDRKKVDVVIPTYKPGKKFSRLLKMLQRQTWPVGKIIVMNTEK
                     SFWNEHGFEGIKNLEVHHLTKEEFDHGETRNRGMRFSRADIVVFMTDDAVPADEHLIE
                     ALVKAFEQRGPEGEAVIMAYARQLPDKDCPLAERYTRSFNYPEESCVKTRADLEQMGI
                     KTFFASNVCCAYDREKFWFQGGFIRRTIFNEDMIFAGKALLQDDYAVAYAAGARVIHS
                     HNYNCRQQFKRNFDLAVSQADHPELFGCVRSESEGIRLVKSTA"
BASE COUNT         4213 a         3576 c         4653 g         4325 t
ORIGIN      
        1 ctgcgggacg ggaaccaggc tctggtggag cccatggtag tagaagaaaa ggtggagatg
       61 ttcaaccttc tggtgaagct gggctttaag gagattgagg tggggtttcc tgcggcgtct
      121 cagatcgaat acgattttct gcgtactctg gtagagcgca ggctgattcc cgatgatgtg
      181 gaagttcagg ttctggtaca gtgcagagag cacctgatcc gccggacctt tgaggcattg
      241 gatggcgtta aaaaggccat tgtgcacatt tataattcca cctccactct gcagaggaat
      301 gtggtgttcc acaaggatat ggaggagatc aaggatattg ccattgtagg cactaaactg
      361 gtacagcgct atgcggcaga ctgtgatacg aaaatccgtc tggagtattc gccggagagc
      421 tttacgggca cggaactgga gtttgccctt gatatctgta ctgccgttca ggaaacctgg
      481 ggcgctacaa aggaaaatcc cattatcatt aatctgcctt ctacggtgga gatgacaact
      541 cccaatgtat atgcggatca gattgaatgg atgaatactc atttcaaaaa cagagaaagc
      601 attatcttaa gcgtacatcc tcacaatgac cgaggcgaag gcgtggcttc tacagagctt
      661 gccatgctgg cgggggcaga ccgtgtagag ggaactctgt ttggaaacgg cgagcgtacc
      721 ggaaatgtgg atattctgac cgttgcttac aatatgttct ctcagggtat taacccggag
      781 ttaaacattg aaaatatccg tgagattgcc gagatctgtg agcggtgcac caagatggac
      841 attcctccca gacatcctta tgcaggaaag ctggtattta ccgctttttc aggctcccat
      901 caggatgcca tcaacaaggg aatgcaggct ctcagagaga gaaacagcaa gtattgggag
      961 gttccctacc ttcccattga tccttccgat atcgggcggg aatacgagcc gatcgtccgc
     1021 atcaacagcc agtccggaaa gggcggcgta gcctttgtaa tggatacctt ctatggcttc
     1081 aagctgccaa agggcatgca taaggagttt gcggatgtga tccagaagat ttcagagaag
     1141 caaggtgagg tagctcccga gcagatcatg gatgaattta agaaggaata cttagagcgc
     1201 aaggagccga tccatttccg caagtgccgg atcacggata cggagacggc agacggcgag
     1261 tttgctactc ttgcaaaggt cgtttatacg gatcatggca tggaaaagca tttcgacggc
     1321 attggaaacg gccccatcga tgcggttcag cgggggctgg aggatgctct cggtatccat
     1381 atcaaggttc tggattatac cgagcatgcc atggcctccg gttccggagc gcaggcagcc
     1441 tcctatatcc atctggtaga ccagaccaca ggaaaggcaa cctacggcgt aggaatcagc
     1501 tccaatatta cccgggcgtc gatccgcggt attttcagtg cggtgaaccg gctgttttnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc tgttttctat ggaaaaaaat
     1681 cctctttcca caggagctga ttcctgatag aataggaggg tgactgaaaa tcagacttaa
     1741 taaaaggggc ttttccggcc cggacttgga ggaaatataa tgcaggaaat acatttagag
     1801 ggacgccaga ggataaaaag ccaggaaatg gcatttaccg gcctgtttgc ggctttgatc
     1861 gcagcaggag cctatatgag gatcgatatt ccggttcagc catttcccat gcattttacg
     1921 ctgcagttct tttttgtgct tctggcaggc tatgtgctgg gggccagacg gggagctgcc
     1981 tgtgtgtgcg tttacctggc agtgggattg gcggggattc ccgtatttgc agcaggcgga
     2041 gggccggcgt atctgatccg tcctaccttt ggttttttga tcggctttgt ggctgcggcc
     2101 tggattacag gaaagctgtg ctggaagcgt ccggggggag gggtggcctg gcggcttttt
     2161 tctgcctttt ggggcatgat ggcgtactat ctgtgcggaa tggtatattt ttatgtgatc
     2221 agtaattatg tgatccatat gccggtaagc tggaaactgg tggtaattaa ctgttttctg
     2281 attacggcgg cagaggactt tatcctgtgt gtgatggcgg ctgtggttgc cagacggctg
     2341 atgccttttg cactagggca gtatcgatag aaaatggata tttatgaaaa ataaaatcac
     2401 gcatgttacc tggacggaac gagtatggcc ggagagaggg cttaacggag cggcccttaa
     2461 atggattgcc atgtgctcca tgttcattga ccatataggg gctgtggtcc tggagtatgg
     2521 actgtattat cagggcggag cagaacggat ggatttcctt ctggaacagt catggggagt
     2581 atttttatat ccgctgtcgc tgcttctgag gattctcggc cgcccggcct ttttgattta
     2641 ctgttttctt cttgtggaag gctttgtaca tacaagggac aggaagaaat acggcagaaa
     2701 ccttcttctg tttggtctga tatcggagat cccgtttaat ctggcagtgg caaacagggt
     2761 gttcgatccc atgtatcaaa atgtgttttt tgagcttttt atcggctttt taatcctttg
     2821 ctggatagaa cgggcggaga agaggacata tggaccaaag gaaatggatc tggtctgtct
     2881 gtacaccttt atcggctgcg gagctgcggt tcttgcagga gcggattata atgccgtcgg
     2941 catactgatt attgtatttt tataccgttt caggaacagc cggaggaagc agactgtggc
     3001 agcaggagtg ctgggtttta tagagacgct gttccctact ctgggagcag gggcgctggc
     3061 agcggtgccc atatggtttt ataacggaaa acggggaaag cagcaggcaa agtttttgtt
     3121 ttactggttt tatccccttc acctttttgt actttttttg atcagatggt attttctggg
     3181 gattcctctg gcgtaggagc gtgacagcca ggaagataac tgatataatg cgggtatagg
     3241 atcaatggtt cgatcacgga tgggaaaagg agcggcaatc atatgaaaaa atacgattat
     3301 attctggtag gaagcggtct ttatgcaggc gtatttgcct ggtatgcaaa aaaacagggg
     3361 aaaacctgtc tggttctgga aaaacgggat cacatagggg gaaatattta ctgcgaggat
     3421 gtggaggata tccatgtaca taagtatgga gcccacattt tccatacaag caataaaaag
     3481 gtgtgggagt ttgtcaattc cctggcagag tttaaccggt ataccaacag tccggtggca
     3541 aattataagg gcgagatgta taatatgccc tttaatatga acaccttcag caagatgtgg
     3601 aacatttcca ctcctgccca ggcgaaggcg atcattgagg aacagaaaaa agaaatccag
     3661 ggagagccga agaacctgga ggagcaggct atcagtctgg taggccggga aatttatgaa
     3721 aaactggtaa agggctatac ggagaagcag tggggcagag actgcaagga gcttccttcc
     3781 tttatcatcc gccgtctgcc ggtgcgttat acttatgaca ataattattt caacgatctc
     3841 tatcagggaa tccccatcgg cggatacaat gtgatgatcc ataagctgtt cgagggatgc
     3901 gagattgaaa ccggagtgga ctatctggaa aacagggagt attacaacag cctgggcggc
     3961 acggtggtct atacgggaac cattgacggc ttttatgatt actgcttcgg acggctggaa
     4021 tacaggagcc tgcggtttga aacagaggtg ctggaggaag aaaatcatca gggagtggct
     4081 gtggtaaatt acacagacag ggaaactcct tataccagga tcattgagca caagcacttt
     4141 gagttcggca ctcagccaaa gactgtgatt accagagagt accccgtatc ctggcaggag
     4201 ggaatggaac cctactatcc ggtgaatgat gagaaaaacc aggcattata ccagaagtat
     4261 gcaaagctgg cagagaaaga ggagaatgta atctttggag gccgtctggg cgagtataag
     4321 tattacgata tggataaggt gatcgaatct gccatgaaga aggcggaaga gatattttaa
     4381 aagagggggc agacaggatg gatgagttaa ggcagctggt agaagcgtat gttccgtata
     4441 atgagcagga agaaagagat cgggagctga tattagagca gattgattcc ggacgggata
     4501 tattgacgag aaataataaa aatgcccaca taaccgtttc cggctgggtg gtaagcccgg
     4561 atcgtaaaaa ggtcctgatg gcctaccaca atatttaccg ctcatgggcc tggctgggag
     4621 gccatgcgga cggagatgag gatgtaagac gggttatcaa aaaggaaata caggaggaaa
     4681 gtgggcttac agaggtgaaa ttcctttcgg atgagctgtt ttcgctggaa atcctcaccg
     4741 tggatggcca cgaaaaaaga ggtgagtatg tatcctctca tctgcatctg aatgtgacct
     4801 tcctcctgga gggggataca gaggcgcctc tggctgtaaa acctgatgaa aacagccgtg
     4861 tgggctggat tccggcagat gagatagaaa gccggtgcag cgaaccgtgg tttgcggagc
     4921 ggatttattc taagttgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnctc
     5041 tcaacgcatc caccagaaca tcatcctctt cctggtttct cactgccagc cgcaggaact
     5101 ggcggttatc agcagagagt ttggaggaca gatcctttac cagacaatgg tactgattta
     5161 acagccgttc tgtgacctgg ccggcgctgt ggccgcctgt aagctcgatc atcagataat
     5221 ctgcctggga ggggattaca cggattccgg ggatttcgcc cagggctttc gcgaagcgtt
     5281 cccgttctgc ctttaccttt accagagccg cggcataatc gtttctgtac ttttcttcaa
     5341 tctgcatata gaactcccct aaggagttga tattccagat cgctacctct tttttcagca
     5401 ggcagatgcg gtctttattg ccgctggcag caatccctaa gcgcagtccg gggataccgt
     5461 aggatttgga aatgctcttt ataaccgtaa gatggggata ctcatctaaa agctcttcct
     5521 gtaaaagggt ggcattttcc tcatctgcaa agtcaacaaa agactcatct gcaatcatat
     5581 gaatgtttcg ctcgcccgtc catcggatca gccgcagcat atcctctctg ggaatgtagt
     5641 ttccggaggg attgtcagga ttgaccacgg tcagacagct gatatcctta tcctcaaaaa
     5701 agtttatcag atcgtcagcc gtataggaaa agttatcgtt atccggataa tatggaacaa
     5761 ctttgtctga ctcataccgg ttggcatatt cttcaaaggt cggacggatc acgccaagtt
     5821 ttccagttgt atgaccgatg agagccttga tcagctcggc ggctccattt cccaccagaa
     5881 tctgatcctg gcggagagag aagtttctgg cagccagaag ggaatttaca tacattccgg
     5941 aaggatactg gcggatcagt acatcaaaat tggatcggat ttcatccagc atctttttgg
     6001 gcggatagta gctgtttacc agataacaga aatccagcat gccgggatag cgccagtagc
     6061 ctccgaaccg gcggctgatt aaggcggtgc gctctttttc aggggcaaac atggattctg
     6121 caatatccag atcctgaata tcgtcaattt cgtaccattt ctggtcaggg ctcagacgtt
     6181 tggccttgat gccgggatca tccagagcca ccagcacctt tagtacctgc tcatagtatt
     6241 cgttgtttcc cagagccttt gtgtatgctt ccaggaaagg aacgtagtgg gtacgggaaa
     6301 attcacggct gaatttataa agatttaccg ttttataata atcctcgatc tctgagaaac
     6361 ggaactgctt tttggagatc atatctacga tgtcatcctg ctcagacagg cgcacgcagg
     6421 ttccgtccat ccaggattcg tattttgcca ccagagccag actgtctctg gaatcattta
     6481 ccagacagtc caggacagaa tcctcaaaga tcacatccga ttccagaaga agggtatcct
     6541 cctccagaag atagtccttt gccagaaaca gggaatagat attgttggtc ttattataaa
     6601 tggggttgtc tacaaagacc acaggagttt tgatatcaag agtgcggata tgctccatga
     6661 gtttctggcc ttcatatccc accacgatca cgatccggga aagggagcag cgctccagct
     6721 ggcttaaggt gcggttgatc atgggaaccc cgtttacctt taccatgcat tttgtgttat
     6781 tttgtgtcag ctgcttcagg cgttttccca tacctgcagc aagaatcatt gcctgcatag
     6841 tgtttgttct ccttcgttat attcgtttgc cgcttccggg cagatagtct ttacagatcc
     6901 cattgggcgt ttggcgcagg ccctttaata ccgctttata gattccggcc cggtaccgcc
     6961 gcatggcagg ctgatttccc aaaagggcca aaagggagtc cagcacaata tgagccagat
     7021 gatttaaaat aatataggcc atatagagga agggatgccg ggaataggtt ttcccgatgt
     7081 caatggcgtt gcggaagccg tagtaatttt tccagctgac cgtgggagca tccccaggtg
     7141 gagcggtacg gtggtttaaa accgcggtat ttacattcag gatccgggac cggctgtgaa
     7201 agcgcatgca gtactccgta tcgtcaaacc agataaaata ttccgttttt ggaaggccga
     7261 tttcccgaac cagagaagcc tttacaagga ggccgcagaa ggtactgata tcgtattcaa
     7321 aggaaggtgt tccataggat gcttcaggaa ccggttcata gaacataaac gtggggcatt
     7381 ttaaaaagcg ccggtggctg gtatcaatgg ctccctttgt ggtgacggtg ccggagtagg
     7441 ccaagtattc gtgactcagg atggcctttt taagctcggc tatatagtca ggtgcgatca
     7501 tggcgtcatc gtcaataatt aaaatccagt cgcagtccgt ggcagacaga cgtttcattc
     7561 cataagcaaa gcctcctgcg cctcccagat tttccttaag acggcagata tcaaaaaaag
     7621 gcccggtaca ggagtcggtt tcagcctgaa atgaggccgg caggtttttc agccagtctg
     7681 ctgtcccgtc actgctgtgg ttgtctacaa tacatatatt ggaaaatggt tcggtctggg
     7741 acagaacgca gcttacgcat tcctttaaga gtgcaaggcg gttataggtt acaatgataa
     7801 ctccgaattt caagggctcc tccttccatg atctgccgat ccgattatgg ttctgattat
     7861 aagtcattta agggggtatg tcaaacggga ttgcaggcgg cagcttccgc aaggggtaca
     7921 gcacattgca aaagcggatg agctccaaat cggattgcac cctgcctgcg aatatgctac
     7981 aatttcagaa ggcaaataaa tggagagatt ggagcaggat ccatatgaat aaaaacaagt
     8041 ccggcaggac acacaggatg cccagtatcc gtaaaaatta tacctataat ctgatttatc
     8101 aggttatgac gcttttgacc ccctttatta ccactccata cctttcaagg gttttagggc
     8161 cggacggaac gggaatccag agctatacca attccgtggt gcagtatttt gccattgcgg
     8221 cggctcttgg aacagcctcc tacgggcaga gggagattgc catgcaccgg gatgataggg
     8281 aagcgctgaa ccgtctgttc tgggagattg agcttctctg cgcagggatg acggcagtct
     8341 gtctggtgtt ctggctgggc gtgatcggat gttcaaagga gtatgcgccc tattacgctg
     8401 cattgtccat gacgctgatc gcagtggcct ttgatatttc atggtttttc ggaggtctgg
     8461 aggaatacgg gctgatcgtt cttcggaata tggctgtaaa atgtgtgggc attgtccttc
     8521 tgttcctttt tatccgcact aaaaatgatc ttctcttata tgtggcttta acggcagcaa
     8581 ccggactttt gggaaatatt tccatgtggg gctatttaaa accctttgtg agaaaaccgg
     8641 atatccgcca gcttaagcct ctgcgccatc taaaggaaac gctggtgtat tttatcccca
     8701 ccatagccac ctctgtctat acgatcctgg ataaaaccat gctgggatgg ttcacagatg
     8761 gggacaagtc ccagaatggc tactacgaat acgccacagg gtttgtgaat atgtcaaaga
     8821 ttttgatcat gtcctttaat gcggttatgt ctgcacggat gtcctatctg tttggcgcag
     8881 ggaaactgga tgaggtgaga gagcgtcttc gcggttctct ggattttgtg cttcttttag
     8941 ccattccgat cacactggga ctttcaggga ttgccgcagg ctttgtaccc tggtttttag
     9001 gtgatgctta ccttccggtg atccctctga tgtatgtctg tgcgcctctg gttttagtgg
     9061 tgggtctaag cgactgtctg ggaagccaga ttttgacacc ggcagggtta agggctcaga
     9121 gcggccgtgc gatcgtagga ggggctgtgg tgaattttat cctgaattta atccttattc
     9181 ctaaatttca gtcctatgga gccgctgcgg cttcagtggc agcggagtgc tttatcacgc
     9241 ttttatatct ccggcttggc aaaggctttg taacactgcc gctcttatgg aaatacagct
     9301 ggagacgcct gattgcgggc gtttgcatgt ttataagcgt agctgtgctg ggccgtacac
     9361 ttgtaaagct ttggggctat agtccggcag tgaccttctt gcagattgga gccggcgccg
     9421 tggtgtatgg catgattctc ctggtgctta gagatccctt tatatggaag caggtggagc
     9481 gcattataaa aaaacaggag catgtttaaa atggaaacag cagaatttta taaagaagaa
     9541 atccgctgcg gctggagagt ggatgaaaag acaaagagga tctgggctgt ccagctggaa
     9601 atgctggacg aggtggagcg gatctgcaaa aaatacggtt tgaaatattt tgcagacagc
     9661 ggtacgctta tcggcgcggt gcgccatcag ggatatattc cctgggatga tgatattgat
     9721 ctggcaatgc tccgggagga ctatgaaaca tttcttaagg tggctccggg agaattaaag
     9781 gagcatctgg tgctacagac gccttacagg gagaaaaatt atctgcgggc ccatgcccag
     9841 ctgcgaaaca gccgtaccac aggctgcagc agggaggaca ggaaagcagg atataactgt
     9901 gggattttca ttgatatttt ccccctggac gccatgcctg aaggccgtgt tctctccagg
     9961 atctgggctt tttccatcca gacattgtgg acgatgcttt acacctggta tcggtttgac
    10021 tattatgaaa atgccacggt atttgggagg attttaaaca ggcttggagc cttcctttcg
    10081 attcctatgg aacgggctgt ttccttttat gaagcagtct gcaggaggtt taatggtacg
    10141 aatgccacaa gggtctgcga tacggtattt atccgccatc tggaaaaaaa tacctggaag
    10201 cgccagtggt ttgatgaaat ggtgtatctt ccctttgaaa accgccggat tcctgccccg
    10261 gcaggctatg atggacggct gaaacaggaa tatggggatt atatgaagcc tgcgaaagcc
    10321 ccaaccatgc atggaagcct gtttctggat ccggatgttt cctatgagga gtatcaaaaa
    10381 aacaactgga gaggtgcaga agagtgaagc tgtcgtctca ttgaaaatgg gcataaaaat
    10441 accggagatc tgtcctgcat cggcgcggac agcctccggt attttgtgtt atctcagccc
    10501 attttccgga acatttacat cggtaggatc ataggtctgt gtttcaggga tcatcttctg
    10561 gatggccgga cggtcaaagg gccccggtac atctgaattg tatacattta cagttgtccc
    10621 cagagcgcag tggtcataaa tccacttggc atcctctgtg gtatagcgga tacagccatg
    10681 agagggagtc gcgcccaggt agttgtaggt tacaggcttt aaggtatagg gatccggtct
    10741 ctcatagata acagagtgga gcaggatcgg aattccctga tccagacggg tgcagtactg
    10801 gcagtattca tccgtgttca tcagtctcca gcggtatttc tcaggcgtct tgtgatcgcc
    10861 cagaggcgtg tcgtcgccgg ttgaacaaag gaatgtcttg taggggatga taaagccgtt
    10921 tgtaccgtcc tgtatgtaaa ccgtggtaca gttcatctgc ttgttgatct taagcataaa
    10981 aggcccctga gcgttcagat aaggctccag atcctgtacc agctttccgt ctgcttcaaa
    11041 ataatacttg tatccgtcaa tgtactgcca gccggtaacg atcgcgttgt ctaccacata
    11101 gtagcggtct ctgtcattcc atacccagcc ggtgaacaga tctccggtac cgttgctgaa
    11161 tacgggggtg ccgtttacaa actggatgcc gtccggtgcg gcagatacaa ggggagcgtc
    11221 cattttatag acagcgcctt ccgtgccttt gccccagagg gaaaaacgca ggccggtaat
    11281 gtagcgtccg caggccatgg ctccgttcgt tccgccgttt tttgaccagt cgcagaccgt
    11341 tccatccgac agggttgcgc agtagtatac gtcaaagctg tttccgaata cgccgtttaa
    11401 acggatctgg atcgcttcaa tggggcagtc tgcagcccag gatgtgtggc cgccgttcat
    11461 ggcccagtta ctccagccgc ggggagcgct gtaggtgcgg taaagtacgt cgccgggaag
    11521 attattggcg taaatggaca tactaataaa gccgccttca ccgggataaa tggtggtatc
    11581 gtcacggatg ggatcggtcc aggtcatatc cggacggaga agctgcacct gtaaaaaggc
    11641 ctgattggaa ggaacctggg tgctttcctc cggggggact tcctgagcct gtccggctgt
    11701 ttcgcctgag gcctgtccct ctgttcctgc ttctggagct ggggtttccg tttccggaga
    11761 ggcagtttca gactgggatg attcctgccc gccggttccg cttcccggct ctacgatgat
    11821 gccggggccg ttctgggtgg tgtcgccgga aacgccggga gcctcctgcg aaacctcggg
    11881 agcctgtgaa acgccggggc catggttgac gtcctgatcc gccagagctg taagggcatt
    11941 tcctgacagc agggaagcgg taagaactcc caaagccata tatcgaaggg atcttcgcat
    12001 gataaaaaac ctcctgaatg gatgatgtac tgtaacctaa tttaacatat ttaaaggaaa
    12061 aatgccatag atgatttact tttttaagct cttattgtaa aatcgtaaat aaaatgaccg
    12121 gagaatgtaa gaaacaggtg atcgtatggc agaaacagac tcacgcatca atatcatata
    12181 tgcttccaat gacggatacg caggccatct ggcggcttct ctctgctctc tgttagacca
    12241 taaccgcagg gcagagcaca tcgacattta tgttttgtct gtagggatgt gcacagagta
    12301 tcagaaccgc cttaaaagcc tggcagcttc ctttgggcgg agcctgagcg tagtggagct
    12361 gggaaattta aaggagcgtt ttccttataa aatagataca agaggctttg atatcagcgc
    12421 catgggtcgt ctgtttgcac ccaaggttct gcctgaaagc gttgagcggg cgttgtatct
    12481 ggactgtgat accattgtgt gcggttcgat tgagcctctc tatgagacag agctggggga
    12541 atgcctggca ggaatggtga tggagccgac ggtatatcag gaaatgaaag agaccatagg
    12601 gatgaagccg gaggatgcat actttaattc cggggtgatc ctgatggatc tggccagatg
    12661 gagagctgag gatgttttaa aaaagctcct tgatttttac ggcgcccatg cagggagcct
    12721 ctttgcctgc gatcaggata ccataaacgg cgcgttaagg gggagaatct gttccctgcc
    12781 gcccaaatac aattttttca ccaattaccg ctattttcgc tatcagactc ttaaaaagct
    12841 ctgcgccgct tatgaagagg tgggagagca ggcgttttat gaggcgaagc gtgctccggt
    12901 ggtgatccat tatctggggg atgagaggcc ctggatcagg ggaaaccata atcattataa
    12961 aaaatattat aaaaagtatc ttaccaggac gccctggaag gatatgcctc tcacagaggg
    13021 aaaattcctc tatatgcagc tgtggtggtg ctttaaccag atgacgcggc tgtgccccgc
    13081 ccttcgtctt gccataagca gacggcttgg aatgcgggtg atcgatgcaa gaaagacagg
    13141 aaaggaaaga aagtaagttt acaatggata gtgacacgga cagggataag atctgctgta
    13201 ttattttaaa ctataatgat gcagaaactg cagcaggact ggcagaggag ttaaaggaga
    13261 gccgtctgat tgacgatatc cttctggtgg ataactgctc caccgatgat tcatgggaga
    13321 agtttaagaa gctggagctt ggatggaaaa aagaggaaaa ggaagataaa gaggatattc
    13381 ttcccagcct gtggttgata cggacggatg gaaacggcgg ctatggcagc ggaaaccagg
    13441 caggaattga ttatgccatg gagtatctgg aaccggatta tattataatt gccaatccgg
    13501 acgttcattt tttcgatgac tgtgtaaaac ggctgaaaga ggttcttgag gccagagagg
    13561 atggagccat agcatcagcc atggtgacag atcctgaggg ccgggaattg ttttcctact
    13621 gggacctgca gcctatgtgg aaggatcttc tggatacggc tccgttagcc agacggatgt
    13681 ttgcgcctgt attaaaaacg cctcttttgg aactgcctga aggcggggac ggcaatacgc
    13741 ggatcgtggg cgcactgccc gggtcttttt ttatgataaa gacaggctgc tttacaccgg
    13801 gtgatttaga ggagctgttt gacagaaatg tgttccttta ctatgaggaa aagattttag
    13861 ggcagaagct gaaaaacctg ggtttaaagg cacttctggc agtggatgcc agatatgtcc
    13921 atgcccattc agtaaccatt gataaatgct ataaaaaggc ggctgaccgt caaaggctgc
    13981 tccatgagag caagctgtat tattataaac attatcttca taaaggcccg acttccctga
    14041 cagccgccgg gatgtttctg ggacttgtac tggcagaggt aagtgttttg accggccttc
    14101 taaggattaa ggccggaaga tagggatttg agaaaaagag gatgacacag atggataagg
    14161 tgctgcttgt aataccggca tataatgagg aaaagaatat agagcgggtg gtagatcagc
    14221 ttacagaaag ttttccggag cttgactata tcgtagtcaa tgacggctct aaggaccgga
    14281 ctgcagagat ctgcagaaaa cggggctacc gtcttttgga tcttccggtc aatctgggac
    14341 tggccggttg ttttcaggcc gggatgaaat acgcctggga aaagggctac cgctatgcga
    14401 tccagtttga cggagacggg cagcaccgtc cggaatttat taaaaccatg agggagaaga
    14461 tggacgaggg ctacgatatt gtgatcggtt cccggtttgt gacagagaaa aaagactggt
    14521 cttttcgcat gctgggcagc cgtatcatcg gcgcggcaat ccggctgact acgggggcac
    14581 acataaagga tccgacctcc ggcatgcgtt tgtttaaccg gaaaatgatc ggggaatttg
    14641 ccctcaatct gaactacggg cctgagccgg ataccatctc cttcctgatc caccaggggg
    14701 ccagagtggc tgaggttcag gtgacgattg atgaccggac tgccggggaa agctatctta
    14761 agccggttac ggctgtaaaa tatatggttc gtatgctctt atccattctg gtgatccaga
    14821 atttcaggaa acggcagcag gatgcaaaaa cgaaaggata aatgagaatg aaacggatga
    14881 cgcaggctct gttatataag ggggatcata taaactgggc aaatgtggta tggaatattg
    14941 caggaagctt tgtctatgca tttgcaagca tggttttatc ctttcttgta atccggctgg
    15001 caggagaaga acagggagga attttttcct tcggcttttc gactctgggc cagcagctgt
    15061 ttttagtagc ctattttggc atccgcccct tccacattac agacggagcc ggcgaatata
    15121 cctttggaga ttacagggag cacagaaata taacctgcct gatcgctctg gctgccggag
    15181 ctgtgtttat gatgatccgt cacggggcgt atcttcgggg agtcggtgat tataatggag
    15241 aaaaatgcac cattctgctc cttttggtgg tctataaggt gattgacgga tacgcggacg
    15301 tgtatgaatc ggagtttcag cgccagggaa gcctgtatct gacgggaaaa tccaattttt
    15361 tccgaaccat tctttccgta tctgtcttta cggtcatact ggctgccttt gaaaacctgc
    15421 ttttggcttg tctggcagca gtagcggccc agatcggagg agtcatcctt tttaatctgg
    15481 atgtgatcca tgctctttcc gctgtgaaat gggaggcaaa aaagggacag tgcagaaaac
    15541 tgtttttaag cacaggcttt ctctttgtgt ctgtatttct tgatttttat atcttttcct
    15601 ctgccaagta cgccattgac gccaggatga acaatgcggc ttccgggtat tttaacttga
    15661 tttttatgcc tacctctgtt atctatatgg tagcgaattt tgtgatccgc ccctatctga
    15721 ccgtcctgac agaattttgg gggaaggatg acaggagggc tttttttaat acccttaaaa
    15781 ggatcagtgc ggtgattgcc ggtcttacgg tgctggcagt gggaggaacc ttatgtctgg
    15841 gaaaatgggt tctctccgtg atggagcaga tcatgggagg agatccgggc gtgctgacgg
    15901 cacatttctg ggcatttaca ggaatcgtgc tgggaggcgg tttttatgcc tttgccaatc
    15961 tgatgtacta tgctctggtg attatgcgca ggcagaaaac cgtatttttc gtatacggcg
    16021 caggcgctct ggcagcagtg gtcttatcag gcgctctggt aggagccttt gggatcggcg
    16081 gaggcgcact gtgctatctg cttcttatgg cgggcctttg cgcaggcttt ggcatctgta
    16141 cggcaatcgg gctgaaaaag tgagtgttta ataaggaggg cctatggaag atagaaaaaa
    16201 ggtggatgtg gtgatcccca cttataaacc gggaaagaaa ttttcaagac ttttgaaaat
    16261 gctgcagcgc cagacctggc ctgtggggaa gatcatcgtg atgaatacgg agaaatcatt
    16321 ttggaatgaa cacgggtttg agggaattaa aaacctggag gttcaccatc tgacaaaaga
    16381 ggaatttgac cacggagaga ccagaaacag aggaatgcgc ttcagccggg cagacattgt
    16441 ggtatttatg acagatgatg cggtccctgc agatgaacac ctgatcgagg cgctggtgaa
    16501 ggcctttgaa cagagaggac ctgagggaga ggctgtgatc atggcttatg cgagacagct
    16561 cccggataag gactgccctt tggcagagcg gtatacaagg agctttaatt acccggagga
    16621 aagctgtgta aagacaaggg cagatctgga gcaaatggga atcaagacct tttttgcctc
    16681 taatgtgtgc tgcgcttatg accgggagaa attctggttt cagggcggct ttatccgcag
    16741 gacgattttt aatgaggata tgatttttgc gggaaaggcc cttttgcagg atgactacgc
    16801 agtggcttat gctgccggcg cccgtgtgat ccattcccac aactataact gcaggcagca
    16861 gtttaaacgc aatttcgatc tggcggtgtc tcaggcagac catccggagc tctttggctg
    16921 cgtgcggtcg gaaagcgagg gaatccgtct ggtgaaatcc acagccc
//
LOCUS       DBMDUH010000044        16752 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_1976_length_16752_cov_5.336977, whole genome shotgun sequence.
ACCESSION   DBMDUH010000044 DBMDUH010000000
VERSION     DBMDUH010000044.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 16752)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 16752)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16752
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1976_length_16752_cov_5.336977"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="ACZPCG_05575"
     CDS             complement(<1..270)
                     /locus_tag="ACZPCG_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HIR1284578.1"
                     /translation="MLTEPMTLYKLMNLYMLKQVNFPLSNAQLTSFFTEHEYTTYFTL
                     QQALNELEEAGLIHKEASHSSTRYEITKEGEETSMYYFTSRKSEKA"
     gene            438..4016
                     /locus_tag="ACZPCG_05580"
     CDS             438..4016
                     /locus_tag="ACZPCG_05580"
                     /inference="COORDINATES: protein motif:HMM:NF040159.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5717 family protein"
                     /protein_id="HIR1284579.1"
                     /translation="MRERINRLARGIVDGGVPGLMIKPERLEGSFGAGQTVKGEFLVQ
                     STNDLYIKGLVYSSNERVRAVNEAFGGLRSHIIYEVDTRCMEDGDEIKGSFYLVTNGG
                     EREIPYSLRIQTGTGGEELASLKTARDFANLARKNIELALRLFEYQDFIQAPFMQEPE
                     ARSIYEGLRGRNGRKNLLEEFLVALHVKEPVRISTDTSPRCYRDLEEEKEDFIELALS
                     GWGYKEAVLQAEGAFISLETSSVTDRDFTENRCQIPYRILPDALHGGKNMGRIVIRTP
                     RREFVIPIEAERRKRRDPYRETAEDKKGFLEYIQKRLLYELAASTDREWEEELLQETE
                     KLRILYPKDGRVRLLRAEHLMLSGRRDTAQAALEEVREEVLKEQEKMPWLYCYYEYLC
                     LLLNPSESRKEELIRYIRKHLWEKGSPSPWLLLLLIRTDETCLQNPLDLYRSMEMLFA
                     NGCRSPFLYGRACRLLTEHPVLFLKLGAFELQVLQLGIREGLLDRETVLKAADFISAQ
                     KYYRKPVWRLAKQLYKAYPERKLLEAVCGLLIRGEQKTGEAFLWYEKAQEAHINITRL
                     YEYFLYSLPDDYNRLLPKEVLLYFSYDKTLDREHRCILYRNILLYMNPSSELYQSYIR
                     EMEQFAMEQLFALRIDSRLAVIYEHMIYQDMIDERAAKVLPAILCSRRIICEEPGMKY
                     VVVRCEELQDEETYALEKGEAYVPARSGHMILMFQDAYGNRYLDVKHRKYPVMDKKEL
                     LDRCAKLAPDHPMLLLRACCQVMGEGIHTLQEAHMLDEAAHLLRLNPVFETGLREQVV
                     EFYCRMEEDDREDKNSANCACLVQMDKEDLSEKARKRICETLIGQDFLREAYDMICRF
                     KITDIDVQLLSRLCSRMILQQLFDQDDWLLSFSYRVFEAGKADSVVLDYLCEHFNGTV
                     SQMYGILIRGVQEHIETYDLEERLLAQMLFTGCCDRIDSVFDLYMSRKKTRESIVKAY
                     FTEKCAQYFLEGKEMDPKVFDYLKKAARMSLNREKLPTLYLLSLTKYFAGIPEISPED
                     KELLLFMTRLLLEQRLIFPYTRDLSRHIPVPEDILDKTMVEYHGDRDRAPWMEVRLLP
                     EETSFHREEMRRVYQGIYVKEMVLFEGETLEYQIYERGSRGRVLTAEGRLTCDHKLDG
                     RENSRFACLNRMGAALKEKNEEKLAGAMEDYLKRSAVLGSLFPVKE"
     gene            4033..5256
                     /locus_tag="ACZPCG_05585"
     CDS             4033..5256
                     /locus_tag="ACZPCG_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5716 family protein"
                     /protein_id="HIR1284580.1"
                     /translation="MDGLVIGLDLNDEYTQISCYGKEISWTIPTVICRKKEEEIWLTG
                     EEAYAATLLGEGVIVDKLLKMVQKEGTSTIGGVCYTGALLLRIFLQQVLEYPLKEEGK
                     KTVSQLVVTMQEMDARLTDTLMYCADAMGISRDRVHVISHTESFVYYVLSQKRELWSN
                     QVGLFELAADRLCYYEMKVQRGMRRNMVQAEAQNQEEAFDLDILGTPSGQKLADQILT
                     SCGEKLLSKKLFSTVFLTGKGFERQDWAGGFMKLVCSRRRCFVEKELFSRGAAFKGQD
                     YLNSATEYPYIFVCEGRLKAEVDLKVMQKGKEILLAVASYGDNWYESKSSLELILEDE
                     KEIEFIISHLDSRKKKVVKVPLSGFPDRPPKTTRIEMNIGFKDENTMVTVIKDRGFGD
                     LFPASGAVVRQEVSL"
     gene            5253..6065
                     /locus_tag="ACZPCG_05590"
     CDS             5253..6065
                     /locus_tag="ACZPCG_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284581.1"
                     /translation="MSLLLCRREPVKHPLYIEVLGIHIYSSQELCYVICNHPLLVMDG
                     FVGKALYSFVKNELGMGFVAGRMEKLSETGSRPEEALYLFLTECDYCTEKESQRFRQT
                     AAAYRSLPAPRYEKAVADYLFGKKQYGRASVRYEKLLAETEEKKEDPAFMGALYQSLG
                     AAYAQMFQFHRAYRAYDKAYGLDHDREVLKRIYFLSVLAPELEAADTYGPLFKPEFKG
                     AWEEELRQARTDGEQAEEVRGLRTLMKRDMPYRMEGAARIVGRWKSEYRKMV"
     gene            6433..9075
                     /locus_tag="ACZPCG_05595"
     CDS             6433..9075
                     /locus_tag="ACZPCG_05595"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003504275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HIR1284582.1"
                     /translation="MKELEKTYDPAAIEDRLYEKWLNGKYFHAEVNRSKKPFTIVMPP
                     PNITGQLHMGHALDNTMQDILIRYKRMQGYEALWQPGTDHAAIATEVKVIDKLKKEGV
                     SKEDLGRDGFLKECWKWREEYGTRIVKQLHKLGSSADWDRERFTMDEGCSDAVLEVFV
                     KLYEKGYIYKGSRIINWCPVCQTSISDAEVEHVEQDGFFWHINYPVVGEPGRFVEIAT
                     TRPETLLGDTAVAVNPEDERYKDIVGKMLKLPLTDREIPVIADEYVDKEFGTGCVKIT
                     PAHDPNDFEVGKRHGLEEIVILNDDATVNVPGPYFGMDRYEARKAMVEDLKNQGLLVK
                     VVPHSHNVGTHDRCKTTVEPMVKQQWFVRMDEMAKPAIEALKSGRLKFVPESFGKTYL
                     HWLENIRDWCISRQLWWGHRIPAYYCQDCGETVVAKEAPHTCSKCGGSHFKQDEDTLD
                     TWFSSALWPFSTLGWPKNSEDLDYFYPTDVLVTGYDIIFFWVIRMVFSGLEQTGKEPF
                     HTVLIHGLVRDSQGRKMSKSLGNGIDPLEVIDKYGADALRMTLITGNAPGNDMRFYWE
                     RVENSRNFANKVWNASRFIMMNIEKASDLSGVTEDQLTLADKWILSKVNTLSKDVTEN
                     LDKYELGIALQKVYDFIWEEFCDWYIEMVKPRLYNEADDTKAAALWTLKHVLIQALKL
                     LHPFMPFITEEIFCNLQEEEETIMLSSWPLYEEKWNFASEEQAVETIKAAVRAIRNVR
                     TSMNVPPSKKATVYVVSEDEDILGIFEHSRSFFATLGYAGEVILQKDKAGIGEDAVSA
                     VIHKAVIYMPFAELVDIEKEIERLKAEEKRLNGELARSNGMLNNEKFVSRAPEAKIAE
                     ERAKLEKYTQMMEQVKARLAQLVK"
     gene            9160..9456
                     /locus_tag="ACZPCG_05600"
     CDS             9160..9456
                     /locus_tag="ACZPCG_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284583.1"
                     /translation="MDILIVMCMGILVGRLFRSGRIKKVNELLSLACTFVLIFAMGGT
                     LGQRENFVREFLSLGGASFLFFAFPTFFSIVFVYILTRNFMGRKKYETRGKKEK"
     gene            9453..10049
                     /locus_tag="ACZPCG_05605"
     CDS             9453..10049
                     /locus_tag="ACZPCG_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015661 - L-lysine efflux transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903401 - L-lysine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysO family transporter"
                     /protein_id="HIR1284584.1"
                     /translation="MIILFTFISLLGGLAYGIFGFHIPAVSLILKNTDGVLYALMFFV
                     GISIGMHRGILSKIREYHIKIFIIPAGIIAGSIVGGIICSLLLKIPVGQGTAIASGMG
                     WYSLAGAAISELGGAKLGSIAFMSNLMREIFSFFLIPLIALKLNDLTCIAPAGATSED
                     TTLPVMLKYTNEETVVLSVLNGMLCSFAVPVLISVFAG"
     gene            10151..11488
                     /locus_tag="ACZPCG_05610"
     CDS             10151..11488
                     /locus_tag="ACZPCG_05610"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HIR1284585.1"
                     /translation="MMQAEEYLEKIPMWTREKHSLTDIRAFLHEMGDPDRKLSIIHVA
                     GTNGKGSVCAFLTSLYRNAGFRTGTFISPHLVTVRERFLLNNEMVSPGKLQAAFETVL
                     ETVNIMKEKGYSHPSYFEFLFYMAMALFADETPELVILETGLGGRLDATNVVENPLAC
                     VITSISLDHIMYLGDTIEAVAGEKAGIIKRQVPVIYDDTVPEASEVIRERAVQMASKA
                     YPVGKGDFSILETKDQGLSIRAEGEAAQAFAAAGPLVLEIPFEAPYQAENAMLAVKTA
                     VVLGQRERGFRLTEAQITEGIRTARWAGRMERAGENLYLDGAHNPGGIKAFIQAAASM
                     AARQKKKAYLLFGAVSDKDYRAMARLLCEGISWAGIGVVHIDSSRSMDTEVLAEAFSQ
                     AYKGPVRAFETAGEALREMKKQAGDELLFCAGSLYLIGELKVQLKGMERREDT"
     gene            11485..11631
                     /locus_tag="ACZPCG_05615"
     CDS             11485..11631
                     /locus_tag="ACZPCG_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284586.1"
                     /translation="MIDFEEEIQRFKPSLDVEAVEDAIVKSDLTDMTDIMMEMIRERD
                     GKRR"
     gene            11631..12935
                     /locus_tag="ACZPCG_05620"
     CDS             11631..12935
                     /locus_tag="ACZPCG_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR1284587.1"
                     /translation="MEIEAKNRQIANSYYNLGLEKARIRDLSGAAQCLKKSLHFCKYQ
                     IDARNLLGLIYYENGEVADALVQWVISMSLQPENNLADHYLERIQRKPGQLEAESQAV
                     KTFNQALWHAQNGSDDLAALQLARVVSAKPHFVKAHLLLALLYMRREDYNKAGRSLYK
                     ILQIDKSNSKALYYMSIVKQNTGRAEAEKRRMVKAFSHRQMEDDDVIIPNTYKESTGL
                     STVFHIILGLIFGLVAFYVLVLPAVENRLNRQRDDELMVYMQKLNSANQENDILAQEN
                     EDIEAKMAEVQEQLDTLTTGNTSIIAQYQSLIWVLQSYRTGDMVSAAKTFAESSFDLI
                     EDANIQGIVDNIRQEMTGSIYQTLVDKGLQLWNGGNKTQAQEYFLASLKIRPDNPEAM
                     FYAGRLYQDAGDTENANALFDKVVGEHGDTEYAERARNARGY"
     gene            13041..13388
                     /locus_tag="ACZPCG_05625"
     CDS             13041..13388
                     /locus_tag="ACZPCG_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR1284588.1"
                     /translation="MDAGTITYEAYEDTLIIHMPKELDHHNCRKLKTDTDLMLEENCV
                     RRIIFDFEKTEFMDSSGIGVLLNRYKEMKASRGSVCYYRASRRVRRLMELSGICRLME
                     GYESREQAVGALK"
     gene            13405..13839
                     /gene="spoIIAB"
                     /locus_tag="ACZPCG_05630"
     CDS             13405..13839
                     /gene="spoIIAB"
                     /locus_tag="ACZPCG_05630"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA];
                     GO_process: GO:0042174 - negative regulation of
                     sporulation resulting in formation of a cellular spore
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HIR1284589.1"
                     /translation="MEGDREHIRLEMESRSSNEEFARVVAAVFMSRLDPTLEEMDDVK
                     TAVSEAVTNAVIHGYREKCGTVVMELTADRTERSLTVEVTDQGTGIEDIKKAMEPMYT
                     TDRSGERSGMGFAFMEAFMDEITVESEAGCGTKVTMKKYIGR"
     gene            13845..14573
                     /locus_tag="ACZPCG_05635"
     CDS             13845..14573
                     /locus_tag="ACZPCG_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HIR1284590.1"
                     /translation="MEETIRQIQNAHQGDKAARDRLVTENTGLVWSIVRRFAGRGYEL
                     EDLFQIGSIGLMKAIDKFDTNYEVKFSTYAVPMIAGEIRRFMRDDGMIKVSRSVKELG
                     FKARNAAEELTKILGREPTIEEIAEKTGASREEIAASLEAAAEVESLYKPLGNSGDKE
                     LLLMDRLEEEHDSHEELLNHLVLKELMEGLTDQHREIILRRYFYSQTQTQVAKALNIS
                     QVQVSRLEKRILKEMRKRYEEGSS"
     gene            14688..14843
                     /locus_tag="ACZPCG_05640"
     CDS             14688..14843
                     /locus_tag="ACZPCG_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284591.1"
                     /translation="MKSGKYKWIIGILAAAAILLGVFAWYAVWGIRDKGYENEGVMVY
                     IQKCQGV"
     gene            14846..15472
                     /locus_tag="ACZPCG_05645"
     CDS             14846..15472
                     /locus_tag="ACZPCG_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AA"
                     /protein_id="HIR1284592.1"
                     /translation="MTHTVYVKLDEITELTHKDILLKDVAQVYCRDQNIQNKCSVCRI
                     MTVRSDEAHRYVMSALDVTKKLEALDPGIEVEHMGKTDFIIAYKPAAPPAYGRQWCKT
                     ACICLISFFGAAFAIMTFNNDVSVTSVFHEIYRLVMGYEAAGFTVLEASYCVGLSLGI
                     MVFFNHISAGKVNTDPTPLEVEMRLYEENISKTLIDNAQRKEKKIDIS"
     gene            15459..15896
                     /locus_tag="ACZPCG_05650"
     CDS             15459..15896
                     /locus_tag="ACZPCG_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003504317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AB"
                     /protein_id="HIR1284593.1"
                     /translation="MTFLKYAFLIFFGICAGGVIAAGVYAFLAMIGVFVRLMGRTNTG
                     KKARLYEKWIIAGGILGNAADIYEIPMILGKGPGTLFLTAAGLSIGVFVGCLAMSLAE
                     TLKALPAMSRRIGLAAGLQYLILGIAAGKFAGAWIYFWKGLAV"
     gene            15914..16369
                     /locus_tag="ACZPCG_05655"
     CDS             15914..16369
                     /locus_tag="ACZPCG_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVA/SpoVAEb family sporulation membrane
                     protein"
                     /protein_id="HIR1284594.1"
                     /translation="MEIDKKKYEEYVKRITPVHAFWPTLGKAFLTGGLICTLGQFINN
                     WLMNSRGLEQEAAGAWTSLTLILLSVLLTGFNIYPKLVTFGGAGALVPITGFANSVAA
                     PAIEFHAEGEVFGVGCKIFTIAGPVILCGVLASWALGAVYWIGGMMGIF"
     gene            16403..>16752
                     /gene="eno"
                     /locus_tag="ACZPCG_05660"
     CDS             16403..>16752
                     /gene="eno"
                     /locus_tag="ACZPCG_05660"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591041.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HIR1284595.1"
                     /translation="MKRLEIASVTGREILDSRGNPTVEAEVHLTDGTMGRGTAPSGAS
                     TGEFEALELRDHDLDRFGGKGVLKAAEHIRTDIHAILRGMDPCNIYEVDHAMIKADGT
                     ADRSKLGANAILAVS"
BASE COUNT         4536 a         3412 c         4803 g         4001 t
ORIGIN      
        1 cgctttctcc gactttcggc tggtgaagta atacatcgag gtttcttccc cctcctttgt
       61 aatctcatag cgtgtgctgc tgtggctggc ttccttatgg atcagtcctg cttcctccag
      121 ttcatttagg gcctgctgaa gggtaaaata ggtagtatat tcgtgctccg taaaaaagct
      181 ggtcagctgg gcgttgctta aggggaagtt cacctgcttt aacatataca gattcatcag
      241 tttatatagg gtcataggct cagtcagcat atcgcgcact ccattgaata aaattgttgc
      301 aattcttacc gtctatcata ccaagtttaa gggggtaaat caagctgtag tgcagatttc
      361 tcttttaaat tatatgaaaa tgtgttatca tgaaacgtaa gcaaaaagca taagaggaag
      421 acacaggtgg taggtgtatg agagaacgga tcaaccgttt ggccagaggc atcgttgacg
      481 gcggtgttcc ggggctgatg ataaagcctg agcgtctgga aggctccttt ggggcaggcc
      541 agacggtaaa gggcgaattt cttgtgcaga gtacgaatga tctgtacata aagggactgg
      601 tctattccag caatgagaga gtacgggctg taaatgaggc ttttggaggc cttcgcagcc
      661 atattatata tgaggtcgac acccgctgta tggaagatgg agacgaaatc aagggatctt
      721 tttacctggt caccaatgga ggtgagaggg agattcctta ttctttgcgc attcagacgg
      781 gaaccggagg ggaggaactg gcatctttaa agacggccag agactttgca aaccttgcgc
      841 ggaaaaatat agagctggct ttgcgtctgt ttgagtatca ggattttata caggcgccct
      901 ttatgcagga accggaagcc agaagcattt acgaaggcct gcggggaaga aacggaagga
      961 aaaatctgct ggaagaattt ctggtggctc ttcatgtaaa ggagccggtg aggatcagca
     1021 cagatacatc gcccagatgc tatagggatt tggaagagga gaaggaagac tttattgagc
     1081 tggcgctcag cggatgggga tataaagagg ccgtgcttca ggcagaggga gcgtttattt
     1141 ctctggaaac gtcttctgtg acagaccggg attttacgga aaaccgctgc cagatccctt
     1201 atcgaatttt accagatgct cttcacggag gaaagaacat ggggcggatc gtgatccgta
     1261 ccccgcgccg ggagtttgtg atccctattg aggccgagag aaggaaacgc cgggatccat
     1321 acagggaaac ggcagaggat aaaaaaggat ttctggaata tatacagaag cggctcctct
     1381 atgaacttgc tgcttctacg gacagagaat gggaggagga gctgctccag gaaacagaaa
     1441 agcttcgcat tctttatcca aaggatggca gggtgcgtct tctcagggca gagcatctga
     1501 tgctttccgg acgcagggat acagcgcagg cggctcttga agaggtgcgg gaggaagtcc
     1561 tgaaggaaca ggaaaagatg ccgtggctgt actgctatta tgaatatttg tgtcttttat
     1621 taaatccctc agaaagcaga aaggaagaac tgatacgtta tataaggaag catctctggg
     1681 aaaagggcag tccatcccca tggctgctgc tccttttgat ccgtaccgat gagacctgtc
     1741 ttcagaatcc tctggatctg taccgttcta tggaaatgct ctttgccaac gggtgcagaa
     1801 gcccgttcct ttacggaaga gcctgccgtc tgcttacaga acatccggta ctgtttttaa
     1861 agctgggtgc atttgaactt caggtgcttc agcttgggat ccgggaaggg cttttagaca
     1921 gggagacagt tttaaaggca gcagatttta tttccgcaca gaaatattac agaaagcccg
     1981 tgtggagact ggcaaaacag ctctataagg catatccgga gcggaaactt ttagaggcgg
     2041 tctgcggttt gctgatccgt ggggaacaaa agaccggaga ggcgttttta tggtatgaaa
     2101 aggcccagga ggctcatatc aacattacaa ggctgtatga gtatttcctt tattccctgc
     2161 cagacgacta taaccgtctg ctgccgaaag aggtgctttt atatttctcc tatgataaga
     2221 ctctggaccg ggagcatcgg tgcattcttt acaggaatat cctgctgtac atgaatccct
     2281 cgtctgagct ttatcagtca tacattaggg aaatggagca gtttgccatg gagcagctct
     2341 ttgccctgcg gattgacagc cggctggcgg tgatctatga gcatatgatc taccaggata
     2401 tgattgatga gcgggcggca aaggtcctgc ctgccatcct ttgctcccgg cgcattattt
     2461 gtgaggagcc cggaatgaag tatgtggtcg tccgctgtga agagctgcag gatgaggaaa
     2521 catatgctct ggaaaaggga gaggcatacg ttccggcccg ttccggccat atgatcttaa
     2581 tgttccagga tgcctatgga aaccggtatc tggatgtgaa gcacagaaaa tatcctgtaa
     2641 tggataaaaa ggaactgctg gaccggtgcg ctaaactggc tccggatcat cccatgctgc
     2701 ttttaagggc ctgctgtcag gtgatgggag aagggatcca tactcttcag gaagctcata
     2761 tgttagatga ggcagcgcat cttctccggt taaatcctgt gtttgagacc ggactgaggg
     2821 aacaggtagt ggaattttac tgccgtatgg aagaggatga cagggaggac aaaaacagtg
     2881 caaactgtgc ctgtctggtt cagatggata aggaggattt aagcgaaaag gcaagaaagc
     2941 ggatctgtga gaccctgatc ggacaggatt ttctcaggga agcttatgat atgatctgca
     3001 ggtttaagat cacagatatc gatgtacagc ttctttccag actgtgcagc cggatgatct
     3061 tacagcagct ttttgatcag gacgactggc ttttatcttt ttcctatcgt gtatttgaag
     3121 caggaaaggc agatagtgtg gttttggatt acctttgcga gcattttaac ggaacggtgt
     3181 ctcagatgta cgggatactg atccggggcg tgcaggagca tatcgaaacc tatgatctgg
     3241 aggaacggct gctggctcag atgttgttta ccggctgctg cgaccgcata gattctgtat
     3301 ttgatctgta tatgagccgc aaaaagacca gagaaagtat tgtgaaggct tattttacag
     3361 aaaaatgcgc ccagtatttt ctggaaggaa aggaaatgga tcccaaggta tttgactacc
     3421 tgaaaaaagc cgccaggatg agccttaaca gagaaaagct tcctacgctg tatctgcttt
     3481 ccctgaccaa atattttgca gggatccctg aaatcagtcc ggaagataaa gagctgcttt
     3541 tatttatgac acgtctcctt ttggagcaga gactcatatt tccgtatacc agagacctgt
     3601 cccgccatat tcctgttccg gaggatatcc tggataagac catggtggag taccatggtg
     3661 acagggatcg ggcgccctgg atggaagtcc gtctgctgcc ggaagaaacc tcttttcacc
     3721 gggaggaaat gcggagggtt taccagggga tttatgtaaa ggaaatggtg cttttcgagg
     3781 gagagacgtt agagtaccag atttatgaaa gagggagccg gggaagggtt ctgacagccg
     3841 agggaaggct tacctgcgac cataagctgg atgggaggga aaacagccgt tttgcctgct
     3901 taaaccgcat gggagcagca ttaaaagaga aaaatgagga aaagcttgct ggtgccatgg
     3961 aggactatct gaaacggtcc gccgtgctgg gaagcctgtt tccggtgaaa gaatagatgg
     4021 aggagacagc ggatggacgg cctagtgatc ggtctggatc tgaatgatga gtatactcag
     4081 atcagctgct atggaaaaga aatatcctgg acgatcccca cggtgatctg caggaaaaaa
     4141 gaggaggaaa tctggcttac aggggaggag gcctatgcgg ccacgcttct gggcgaaggg
     4201 gtgatcgtag ataagctttt aaagatggtg caaaaagaag gcacctctac cattggaggg
     4261 gtgtgctata caggcgccct tctgctgcgt atttttctgc agcaggtttt agaatatccc
     4321 ttaaaggaag aggggaaaaa gaccgtttcc cagctggtag tgaccatgca ggagatggat
     4381 gcaaggctta cggacacact gatgtactgc gcggatgcca tgggcatttc cagagacagg
     4441 gtccatgtga tcagtcacac agaaagcttt gtatattatg tgctgagcca gaaacgggag
     4501 ctctggtcca atcaggtagg gctgtttgag ctggcagcag accgtctgtg ctattatgaa
     4561 atgaaggttc agagaggaat gcgccgaaac atggttcagg cagaagctca gaaccaggag
     4621 gaagcctttg atctggatat cctgggtacg ccttccggac agaagcttgc agatcagatc
     4681 cttacttcct gcggggaaaa acttttgagc aaaaaactct tctccaccgt atttctcaca
     4741 ggaaaagggt tcgagcgcca ggactgggcc ggcgggttta tgaagctggt atgcagcagg
     4801 agaagatgct ttgtggaaaa ggagcttttt tccaggggag cggcgtttaa gggacaggat
     4861 tatcttaatt ccgctaccga atacccctat atttttgtct gcgagggacg tttaaaagca
     4921 gaggttgatt taaaggttat gcagaaaggg aaggagatcc tcctggctgt ggcctcttat
     4981 ggagataact ggtatgagtc aaagagttct ctggagctga tcctggagga tgaaaaggag
     5041 attgaattta tcatttccca tctggattcc agaaagaaaa aggttgtaaa ggtgcctctt
     5101 tccggttttc cggaccgtcc tccaaaaacg acccggattg agatgaatat tggttttaaa
     5161 gatgaaaata ccatggtcac ggtgatcaag gacagaggct ttggggatct gtttcctgct
     5221 tccggggctg tggtaaggca agaggtaagc ttatgagtct tttgttgtgc agaagggagc
     5281 cggtaaagca tccgctttat attgaggtac tggggatcca tatctactct tctcaggagc
     5341 tgtgctatgt gatctgcaac catcctctgc ttgtaatgga cggctttgtg ggaaaagctc
     5401 tttacagctt tgtaaaaaat gagcttggca tgggctttgt ggcaggcagg atggaaaagc
     5461 tttcggaaac cggaagccgc ccggaagagg cgctgtacct gtttttgaca gaatgcgatt
     5521 actgtacgga aaaagagagc cagaggtttc ggcagacagc ggcagcttac cgcagtctcc
     5581 cggccccacg gtatgaaaag gcagtggccg attatctgtt tgggaaaaaa cagtatggcc
     5641 gtgcttctgt ccgctatgag aagcttttag cggaaacaga ggaaaaaaag gaggatccgg
     5701 cctttatggg agccctttat cagagccttg gggcagctta tgcccagatg ttccagttcc
     5761 accgggcata tcgggcatac gataaggcct acggtctgga tcatgacaga gaggtgttaa
     5821 aacgcattta ttttctgagc gttctggctc cggagctgga ggcggcagat acctatgggc
     5881 cgctttttaa gccggaattt aaaggcgcct gggaggaaga actgcgccag gccagaaccg
     5941 atggggaaca ggcggaggag gtgcgcgggc tgcggactct gatgaagcgg gatatgcctt
     6001 accggatgga gggcgcggcc cggatcgttg ggcggtggaa gagcgagtac cgcaagatgg
     6061 tgtgaaaatc tgcttgacaa aacagtgaaa tctatttatg cttaaatgca gacaacagat
     6121 gaaaagaaaa tggctgtgaa aaagaggagt acgtacaggg aaccggcaga gagggcagtc
     6181 cgaaggctga aaggcggccc cggaggaagg tgcggaaggt agcttttgag ccggaagggc
     6241 caacggggga atcagcttcc ctttagtagt ttttcccgta tttcccgcgt taaggggaca
     6301 gagattgccg ggcaggctta ctgcccatat ggcagaatca aaggtggtac cgcgagatga
     6361 tcgccctttg cttttcctta gaggaagagg cagagggcgt tttttatttc ataggaaaaa
     6421 aggaggatat atatgaagga actggaaaag acatatgatc cggcggccat tgaggaccgt
     6481 ctttatgaaa aatggctgaa tggaaaatat ttccatgcag aggtaaaccg cagcaaaaag
     6541 ccctttacca ttgtgatgcc gccgccaaat atcacaggac agcttcacat gggccatgct
     6601 ctggacaata ccatgcagga tattttaatc cgctataaga gaatgcaggg ctatgaggct
     6661 ttatggcagc cgggaacaga ccatgcagcc attgctaccg aggtaaaggt cattgacaag
     6721 ctgaaaaaag aaggcgtttc caaggaagac ctgggaagag acggcttctt aaaggagtgc
     6781 tggaagtgga gagaggagta cggcacccgg atcgtaaagc agcttcacaa gctgggttct
     6841 tctgccgact gggacagaga gcgctttacc atggacgaag gctgctccga cgcagttttg
     6901 gaagtatttg taaaactcta cgagaaagga tacatctaca agggttcccg catcatcaac
     6961 tggtgtcctg tatgccagac ctctatctcc gacgcggagg tagagcacgt agaacaggac
     7021 ggcttcttct ggcacatcaa ctaccccgta gtgggagagc cgggccgctt tgttgagatt
     7081 gcaaccaccc gtccggagac gctccttggc gatacggctg tggctgtcaa tccggaggac
     7141 gagcggtata aggatattgt aggcaagatg ttaaagctgc ctttaaccga ccgtgaaatt
     7201 cctgttattg cagatgaata cgtagataag gagtttggaa ccggctgcgt aaagattact
     7261 ccggcccatg accccaatga cttcgaggta ggaaagcgcc atggtttaga ggaaatcgtt
     7321 attttaaatg acgatgccac agtaaatgta ccgggcccat actttggcat ggaccgctac
     7381 gaggccagaa aagccatggt agaggactta aagaatcagg gacttctggt aaaggtggtt
     7441 ccccattccc acaatgtagg tacccacgac cgctgcaaga ctaccgtaga gcctatggta
     7501 aaacagcagt ggtttgtgcg catggatgag atggcaaagc cggccattga agctttaaaa
     7561 tccggacggt taaagttcgt gccggaaagc tttggaaaga cctatcttca ctggcttgaa
     7621 aatatccgtg actggtgtat ttccagacag ctctggtggg ggcaccggat tccggcctat
     7681 tactgccagg actgcggaga aacggttgta gcaaaggaag cgccccatac ctgctccaag
     7741 tgcggcggca gccattttaa acaggatgag gatacgctgg atacctggtt ttcttctgct
     7801 ttatggccct tctccactct tggatggcca aaaaactcag aagatctgga ttacttctat
     7861 cccacagatg tgctggtaac gggctatgat attatcttct tctgggtcat ccgtatggta
     7921 ttctcaggcc ttgagcagac cggaaaagag cccttccaca ctgttttgat ccatggactg
     7981 gtaagagact cccagggccg caagatgagc aagtcccttg gaaacggcat tgatcctctg
     8041 gaggtaattg acaaatacgg cgcagacgcc ctgcgtatga cattgattac gggaaatgca
     8101 cccggaaacg atatgcgttt ctactgggag cgtgtggaga acagccgaaa ctttgcaaat
     8161 aaggtctgga atgcttcccg ctttatcatg atgaacattg aaaaagcttc cgacctgtcc
     8221 ggcgttacag aggatcagct tacgctggca gataagtgga tcctttcaaa ggtaaatacc
     8281 ttatcaaagg atgtaacgga aaatctggac aagtatgagc tgggaattgc tctccagaag
     8341 gtatatgact ttatctggga agagttctgc gactggtaca ttgagatggt aaagccaaga
     8401 ctgtacaatg aagcagacga tacaaaggct gccgctctct ggaccttgaa gcacgttttg
     8461 atccaggcat taaagcttct tcatccattt atgcccttca tcacagagga gatcttctgc
     8521 aacctgcagg aggaagagga gaccattatg ctgtcttcct ggccgctgta tgaggaaaaa
     8581 tggaacttcg cctctgagga gcaggccgta gagaccatca aggctgccgt aagagccatc
     8641 cgaaatgtgc gcacttccat gaacgtgccg ccaagcaaga aagcgaccgt ttatgttgta
     8701 tctgaggatg aggatatttt aggtatcttt gagcatagcc gcagcttctt tgccacctta
     8761 ggctatgcag gagaggtaat ccttcagaag gataaggcag gcatcggcga ggacgccgta
     8821 tccgcagtga tccacaaggc agtgatctat atgccgtttg cagaactggt agacatcgaa
     8881 aaggagatcg agcgtttaaa ggctgaagag aagcgcttaa acggcgagct ggcccgttcc
     8941 aacggcatgt tgaataatga gaaattcgta agccgtgcgc ctgaggcaaa gattgccgag
     9001 gagcgtgcaa agctggagaa atatacccag atgatggagc aggtaaaggc tcgtctggca
     9061 cagcttgtaa aataacagat cccgtcataa ccggggctgc cgcgcagttt gcggcggccc
     9121 ccttttgcag atatcagcaa aacagaaagg agtcatccgg tggatatttt aattgtcatg
     9181 tgtatgggaa tcctggtggg acggctgttt cggtccggca ggataaagaa ggtaaatgag
     9241 cttttatccc ttgcctgtac ctttgttctg atcttcgcaa tgggaggaac gctggggcag
     9301 agagaaaatt ttgtccggga atttctttcc ctgggaggcg ccagcttcct cttttttgcc
     9361 tttcccacct ttttttccat tgtctttgta tacattttga cccggaattt tatgggaaga
     9421 aagaagtatg aaaccagggg gaaaaaagaa aaatgattat tctgtttaca ttcatatccc
     9481 ttttaggagg acttgcctac gggatttttg gctttcatat tccggctgtt tccctgatct
     9541 taaagaacac ggacggagtt ttgtatgccc tgatgttttt tgtcggcatc agcattggga
     9601 tgcaccgggg gattttaagc aagatcaggg aataccatat taaaatattt atcattcccg
     9661 caggaattat agccggttcc attgtggggg ggatcatctg ttccctgctt cttaaaattc
     9721 cggtggggca gggaactgcc attgccagcg gaatgggctg gtacagtctg gccggggcgg
     9781 cgatcagcga gctgggaggg gcaaagctgg gaagcattgc ctttatgagc aatctcatgc
     9841 gggaaatttt ttcttttttc ctgattcccc tgatcgccct taagctcaat gaccttacct
     9901 gtattgcgcc tgccggggcg accagcgaag ataccaccct tcctgtgatg ctaaaatata
     9961 ccaatgaaga aacggttgtt ttgtcggttt taaacggaat gctctgctct tttgcagttc
    10021 cggtactgat ctctgtcttt gcaggttagg aaagaaggct gtcgcttcct ggcgaagcag
    10081 ggcgagaaca tgatgttgaa actttgggga aaatcattta aaatagaggg aacaggcagg
    10141 aggatgagta atgatgcagg cagaagaata cttagagaag attcccatgt ggaccaggga
    10201 aaaacattct cttacagata taagagcgtt tctgcatgaa atgggagacc cggacaggaa
    10261 gctttccatc attcatgtgg cgggaactaa cggaaaaggc tctgtgtgcg cctttcttac
    10321 ctctttgtac agaaacgccg gttttcgcac cggaaccttt atctctcctc atctggtaac
    10381 cgtccgggaa cggtttttgt taaataatga aatggtttcg cccgggaagc ttcaggctgc
    10441 ctttgagacg gttcttgaga ctgtaaatat catgaaagaa aaggggtatt cccaccccag
    10501 ctattttgag tttctttttt atatggcaat ggcgctgttt gcagatgaaa cgccggagct
    10561 tgtgattttg gaaaccgggc tgggaggccg cctggacgct accaacgtgg ttgagaatcc
    10621 cctggcctgt gtgattacct ccatcagtct ggatcacatc atgtacctgg gagataccat
    10681 agaagcggtt gcaggagaga aggcagggat cataaaaagg caggttcctg tgatctacga
    10741 tgataccgtg cctgaggctt ctgaggtgat ccgtgagaga gcggtacaga tggcgtcaaa
    10801 ggcatatccg gtgggaaagg gagatttttc cattttggaa accaaagatc aaggactttc
    10861 tataagagct gagggagaag ctgctcaggc gtttgccgcg gcaggccctc tggtgcttga
    10921 aattcccttt gaagcacctt atcaggcaga aaatgccatg ctggctgtga aaacagcggt
    10981 tgttttaggg cagagagaaa ggggtttccg tctgacagag gctcagatta cagaggggat
    11041 ccggactgcc cgctgggccg ggcgtatgga gcgggctgga gaaaatctgt atctggacgg
    11101 agcccacaat ccgggcggca taaaagcctt tattcaggca gccgcatcta tggctgcccg
    11161 gcagaaaaaa aaggcgtatc tgctatttgg cgccgtatcg gacaaggatt acagggctat
    11221 ggctcggctg ctctgtgagg ggatttcctg ggcggggatc ggtgtggtac atatagacag
    11281 cagccggagc atggacacag aggttctggc agaagcattt tcgcaggcat ataaagggcc
    11341 ggtgagagcc tttgaaaccg caggggaagc tttgcgggag atgaagaagc aggcaggaga
    11401 tgagctctta ttctgcgcag gctccctgta tttgatcggg gagctgaagg tacagcttaa
    11461 aggcatggaa aggagagaag atacatgatc gattttgaag aggagatcca gcgctttaaa
    11521 cccagtctgg atgtagaggc ggtagaggac gccattgtga aaagcgatct tacggatatg
    11581 acggatatta tgatggagat gatccgggaa agagacggga aaagacggtg atggagattg
    11641 aggcaaaaaa cagacagatt gccaacagct attataacct gggtctggag aaagcaagga
    11701 tacgggatct ttccggagcg gcccagtgcc tgaaaaagag ccttcatttc tgcaaatacc
    11761 agatcgacgc cagaaatctg ctgggtttga tctactacga gaacggcgag gtggcggatg
    11821 ctctggtgca gtgggttatc agtatgagcc tgcagccgga aaataatctg gcagatcatt
    11881 atctggaacg gatccagaga aagccgggac agttagaagc ggaaagccag gcagtaaaga
    11941 cctttaacca ggccttgtgg catgcccaga acggcagcga tgatctggcg gccctgcagc
    12001 ttgccagagt ggtatcagcc aagccccatt ttgtcaaagc ccatcttctt ctggctcttt
    12061 tatatatgag gcgggaggat tataataagg ccggccgctc tctgtataag atcctccaga
    12121 ttgataagag caattccaag gcgctttact atatgtccat tgtaaagcag aatacgggcc
    12181 gggccgaggc ggaaaagcgc cggatggtaa aggcattttc ccacagacag atggaggatg
    12241 atgatgtgat catccccaac acctataaag agtctacggg gctttctacg gtgtttcaca
    12301 ttatcctggg cctgatcttc ggcctggtgg ccttttatgt gctggtgctc ccggcagtgg
    12361 aaaaccgcct gaaccgccag cgtgatgatg agctgatggt ctatatgcag aaattaaaca
    12421 gcgccaatca ggaaaatgat attctggcgc aggaaaatga ggatatcgaa gccaagatgg
    12481 cagaggtaca ggagcagctg gatacgctca caaccggcaa caccagtatt attgcccagt
    12541 atcagagcct catctgggtg ctccagtcct accgcaccgg ggatatggta tcggcggcaa
    12601 agacctttgc tgagtccagc tttgatctga tcgaggatgc gaatattcag gggattgtgg
    12661 ataatatacg tcaggagatg acaggaagca tctaccagac gctggttgat aagggactgc
    12721 agctctggaa tggcggaaat aagacccagg ctcaggagta tttccttgca agcttaaaaa
    12781 taaggcctga caaccctgag gctatgtttt atgcagggcg tctctatcag gatgcgggag
    12841 atacggaaaa tgcaaacgca ctctttgaca aggtggtagg cgagcatggc gatacagagt
    12901 atgcagagcg ggccagaaat gcaagaggct attaaataaa atacactttt tacggcagaa
    12961 gaaatggatc acaggatcgt accttctgcc ttttttgtta tagaaaatat ctgcccaaaa
    13021 gagaagaagg aggaaatggg atggacgcag gaacaattac atatgaggca tatgaggata
    13081 cgctgatcat ccatatgccc aaggagctgg atcaccacaa ctgcaggaaa ctgaaaacgg
    13141 acacggatct gatgctggag gaaaactgtg tcaggaggat tatctttgat tttgaaaaaa
    13201 cggaatttat ggattcttca ggaatcggag tgctgttaaa ccgatataag gagatgaaag
    13261 ccagccgggg aagcgtatgc tactacagag catccagacg ggtgcgaaga ctcatggaac
    13321 tgagcgggat ctgcaggctg atggaaggat atgaaagcag ggagcaggca gtgggagcat
    13381 tgaaataaaa aggaggtaca ggatatggaa ggggacagag agcatataag gctggaaatg
    13441 gaaagccgtt ccagcaatga ggagtttgca agagtggtgg cggctgtgtt tatgagccgt
    13501 ttagacccta cactggaaga gatggatgat gtgaaaacag cggtatcgga ggctgttacc
    13561 aatgcagtga tccacggcta tagagagaag tgtggaacgg tagttatgga gctgacggca
    13621 gaccgcacag aacggagcct gaccgtagaa gtgactgatc agggaaccgg aatagaagat
    13681 ataaaaaagg ccatggaacc catgtatacc acagaccgat caggagagag gtctggaatg
    13741 ggctttgctt ttatggaagc ctttatggat gagatcacag tggaatcaga ggccggatgc
    13801 ggaacaaagg tcaccatgaa aaaatatatc ggcaggtgac gcccatggaa gagaccatca
    13861 ggcagatcca gaacgcccac caaggggata aagcggccag agaccggctg gtaacggaaa
    13921 atacaggact ggtgtggagc attgtccgca ggttcgcagg tagaggatac gagctggaag
    13981 atttatttca gattggaagt attggtctta tgaaagccat tgataagttt gataccaact
    14041 atgaagtgaa attttccacc tatgcggttc ctatgatcgc aggagaaatc cggcgtttta
    14101 tgagagatga cggaatgata aaggtaagcc gttccgttaa ggagctgggc tttaaggcca
    14161 gaaatgccgc agaagagctg actaaaatac tgggacggga gcctaccatt gaagagattg
    14221 cagagaaaac aggggccagc cgtgaggaga tagcagcgtc cctggaggcg gcggcagagg
    14281 tggaatccct ctataagcca ttgggaaaca gcggtgacaa ggaactgctt ttgatggatc
    14341 ggctggagga ggagcatgac agccacgaag aactgttaaa ccatcttgtt ttaaaggaac
    14401 tgatggaagg actcacagac cagcacagag aaatcatatt gagaaggtat ttttacagcc
    14461 agactcagac tcaggtagca aaggcgctta acatttctca ggtacaggtt tctcgtctgg
    14521 aaaaacggat cttaaaggag atgcggaaac gctatgagga gggaagcagc tgaaaaatag
    14581 ttgaatagaa acgataaaaa aacccatact accatcagcg gataaaggca gacgaaatca
    14641 tccttgtccg ctgatgatgc atcaaaaccg gaaaggatgt gagacgcatg aaatcaggaa
    14701 aatataagtg gatcatcggc attctggcag cagcagcgat tcttttgggc gtttttgcgt
    14761 ggtatgcagt ctgggggatc agagacaaag gatacgagaa cgagggagtg atggtttata
    14821 ttcaaaaatg tcagggggta tgaatatgac ccatacggtt tatgtaaagc tggatgagat
    14881 tacggaactg acccacaaag atattctttt aaaggatgtt gcccaggtct actgccggga
    14941 tcagaatata cagaataaat gcagcgtatg caggatcatg accgtgcgct cagatgaagc
    15001 ccatcggtat gtgatgagcg ctctggatgt gaccaaaaag ctggaggctc tggatccggg
    15061 gattgaagtg gagcatatgg gaaagacaga ctttatcatt gcctacaagc cggccgctcc
    15121 tcctgcctat ggacggcagt ggtgtaagac agcctgtatc tgcctgatca gtttttttgg
    15181 agcggccttt gctattatga catttaataa tgatgtgagc gtcaccagcg tatttcatga
    15241 gatctatcgt ctggtgatgg ggtatgaggc agcgggcttt actgtgctgg aggcaagcta
    15301 ttgtgtgggg ctgtctcttg ggatcatggt gttctttaac catatttctg ccggcaaggt
    15361 gaatacagat ccgactcctc tggaggtaga aatgcggctt tacgaggaaa acatcagtaa
    15421 aaccctcatt gataatgcac agaggaagga gaaaaagatt gacatttctt aaatatgcgt
    15481 tccttatctt ttttgggatc tgtgcaggag gcgtgatcgc tgcaggtgta tatgcctttt
    15541 tagctatgat cggtgtgttt gtccgtctta tggggcggac aaacaccggg aaaaaggcgc
    15601 gtctgtatga aaaatggatt atagcaggag ggattttggg aaatgcagca gacatttatg
    15661 aaatccccat gatactgggc aaaggccccg gaacgctttt tttaacggca gcaggccttt
    15721 ccatcggcgt ttttgtcggc tgtctggcta tgtcattagc agagactttg aaggctcttc
    15781 ctgccatgag ccgcaggatc ggtctggcgg cagggctgca gtatctgata ctgggcattg
    15841 ctgcagggaa gtttgccggg gcctggattt atttctggaa gggactggcc gtttagctga
    15901 ggaggaagtc attatggaaa ttgataaaaa gaaatatgag gaatatgtaa agcgcatcac
    15961 cccggttcat gcgttctggc cgactctggg aaaagccttt cttacgggtg gcctgatctg
    16021 caccttgggc cagtttataa ataactggct gatgaacagc cggggattgg agcaggaagc
    16081 ggcaggagcc tggacctctc tgacactgat ccttctcagt gttctcctca caggctttaa
    16141 tatttatccc aaactggtaa cctttggagg agccggcgct cttgttccca tcaccggatt
    16201 tgccaattct gtggcggccc cggctattga gtttcacgca gagggagaag tatttggcgt
    16261 gggatgcaag atttttacca ttgcaggccc ggtgatcctc tgcggggttc ttgcaagctg
    16321 ggcgttagga gccgtgtact ggatcggcgg gatgatggga attttttaaa tggtaaattg
    16381 actgtgggaa aggaaaagga atatgaaacg tttggaaatc gcatctgtta caggaagaga
    16441 aattctggat tccaggggaa atcctacggt agaagcagag gtccatctga cagacgggac
    16501 tatgggccgg ggaacagctc ccagcggcgc ttccaccgga gaatttgaag cattggagct
    16561 gcgggatcac gatctggacc gatttggcgg aaaaggtgtt ttaaaggcag cagaacatat
    16621 ccgtacagat atccatgcaa tattgcgggg gatggatccc tgtaatattt atgaggtgga
    16681 tcatgccatg atcaaggcag acggcacagc agaccggtca aagctgggag ccaatgccat
    16741 tttggcggta tc
//
LOCUS       DBMDUH010000045        16639 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_1987_length_16639_cov_6.215625, whole genome shotgun sequence.
ACCESSION   DBMDUH010000045 DBMDUH010000000
VERSION     DBMDUH010000045.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 16639)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 16639)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16639
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1987_length_16639_cov_6.215625"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(161..721)
                     /locus_tag="ACZPCG_05665"
     CDS             complement(161..721)
                     /locus_tag="ACZPCG_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HIR1284596.1"
                     /translation="MENKNERTGLIHIYCGDGKGKTTAAIGAAIRAAGRGFRVLVLRL
                     LKTDDSGEVNALQNIPEIHVLPCVRSFGFSWNMTPEQKEKAAVYYSEQMEKAWKMALE
                     TDENGIPAFDLLVMDEAIGACRQGFICEERLLELLRTRPASLEVILTGRGPSEALLNA
                     ADYVTEMTLRRHPYERGIGAREGIEY"
     gene            complement(691..2607)
                     /locus_tag="ACZPCG_05670"
     CDS             complement(691..2607)
                     /locus_tag="ACZPCG_05670"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HIR1284597.1"
                     /translation="MSFSEAQKRAIRHKDGPALVLAGPGSGKTTVITNRVRYLTEKYG
                     VQAGSILVITFTRAAAREMKERYEKMTGQGSRVAFGTFHSVFFLILKLAYGYQASNIV
                     REEQRIRFIREQAERMQLDLEDPAEFTASVLSEISMVKGEMMPLAHYYPKSCSEEVFK
                     ALYEGYEDQMRREKLLDFDDMLSMCYELFKARPDILTAWQKKYQYILIDEFQDINRVQ
                     YEIVKMLALPQNNLFIVGDDDQSIYRFRGARPELMLGFEKDYPEAERILLDINYRSVS
                     TITESAGKVISNNKTRFPKEICALRGTGRPVDICLWPDPVNETLAIVSQLRDYAQMGI
                     PYEEIAVLYRTNLGPRLLIEKLMEYNIPFHMRDTVPNLYEHWIAKNLFSYIKAARGDL
                     SRANVMTFINRPARYISRDALEGSRISWEQVKSFYQDKNWMLDRIEQLEYDLSMLADM
                     APAGAVNYIRKAIGYDDYIREYAQERRLKPEEFFEVLDALQESAGGFKTWDTWSAHME
                     EYKEELLRQAADREARGEGVSLMTMHSSKGLEFKVVYILDANEGVTPHHKAVLDADLE
                     EERRMFYVAMTRAKDRLHIFYVNERFHKKQTVSRFVEETGLLDTHSETRREAEKERNQ
                     KRGFEWKIKMREPV"
     gene            complement(2609..4057)
                     /gene="gltX"
                     /locus_tag="ACZPCG_05675"
     CDS             complement(2609..4057)
                     /gene="gltX"
                     /locus_tag="ACZPCG_05675"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HIR1284598.1"
                     /translation="MKVRTRYAPSPTGRMHVGNLRTALYAYLIAKHEGGDFLLRIEDT
                     DQERYVEGAVDIIYRTMAETGLIHDEGPDKDGGCGPYVQSERQASGIYMEYAKKLVEK
                     GEAYYCFCTPERLESLRTNVNGEEIMSYDKHCLHLSKEEIEANLAAGKPFVIRQNNPT
                     TGTTTFHDEIYGDITVDNSELDDMVLIKSDGYPTYNFANVVDDHLMGITHVVRGNEYL
                     SSSPKYNRLYDAFGWEVPVYVHCPLITDENHHKLSKRSGHSSFEDLIDQGFVSEAVVN
                     YVALLGWSPEGNQEIFSLEDMIREFDYRRMSKSPAVFDMTKLRWMNGEYIKSMDFDRF
                     YEMAEPYIRRVIKKDLDLKKIAAMVKTRIEVFPDIEGHIDFFETLPEYDTAMYTHKKM
                     KTNSETSLQVLKDVLPLLEAQEDYSNDGLYELLSKYVSDAGVKTGFVMWPIRTAVSGK
                     QMTPAGATEIMEVLGKEESLARIRRGIELLEA"
     gene            complement(4149..6242)
                     /locus_tag="ACZPCG_05680"
     CDS             complement(4149..6242)
                     /locus_tag="ACZPCG_05680"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HIR1284599.1"
                     /translation="MFKSFSMDLAGRKLTVEIGRVAAQANGAAFMHYGDTVVLSTATA
                     SSKPREGIDFFPLSVEYEEKLYAVGKIPGGFNKREGKASENAILTSRVIDRPMRPLFP
                     KDYRNDVTLDNVVMSVDPECSPELTAMLGSAIATSISDIPFDGPCATTQVGLIDGEFI
                     INPTADQKRVSDLALTVASTREKVIMIEAGANEVPEQTVIDAIFKAHEVNQEIIRFID
                     TIVAECGKEKHTYESCAVPEELFAAIRELVPPQEMEEAVFTDDKQTRDKNMDAITARL
                     EEAFAENEEWLPLVGDAIYQYEKKTVRKMILKDHKRPDGRAIDQIRPLAAEIDLLPRV
                     HGSGMFTRGQTQILNACTLAPLSEAQKIDGMDVNETSKRYMHHYNFPSFSVGETKPSR
                     GPGRREIGHGALAEKALVPVLPSEEEFPYAIRTVSETMESNGSTSQASICASTLSLMA
                     AGVPIKEMVAGISCGLVTGETDDDYIVLTDIQGLEDFFGDMDFKVGGTKNGITAIQMD
                     IKIHGLTRPIIEEAIARTRQARMYILEEVMKPVISEPRKHLSPYAPKIKQITIDPQKI
                     GDVVGKQGKVINKIIEETGVKIDISDEGSVNVCGTDEAMIDRAIAIITSIVTDIEAGM
                     VFTGKVVRIMNFGAFVQLAPNKDGMVHISKLSDKRVGKVEDVVNIGDEVTVKVTEVDK
                     MGRINLTMRPSDLAE"
     gene            complement(6436..6702)
                     /gene="rpsO"
                     /locus_tag="ACZPCG_05685"
     CDS             complement(6436..6702)
                     /gene="rpsO"
                     /locus_tag="ACZPCG_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HIR1284600.1"
                     /translation="MISKEKKAQIIAEYGRKAGDTGSPEVQIAILTERITELTAHLQQ
                     NPKDHHSRRGLLMMVGQRRGLLDYLKKTDLEGYRALIEKLGIRK"
     gene            complement(6843..>7529)
                     /gene="ribF"
                     /locus_tag="ACZPCG_05690"
     CDS             complement(6843..>7529)
                     /gene="ribF"
                     /locus_tag="ACZPCG_05690"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HIR1284601.1"
                     /translation="YLVEYPFDDQVRRMAPEDFVVQILSGRMKAAAIVVGPDCSFGYQ
                     GAGNAELLRKLAASCGYSLYVIEKEQDEDRHRDISSTYIREELACGNVAKANELLGEP
                     YAIHGKVVHGNHIGGAVLGFPTANLIPPAIKRLPRYGVYVSRVLVDGVYYQGVTNIGK
                     KPTVEGDNPPGAETYIMGLDRDIYGSTIEVQLLDFIRGERKFDSLEELKEQISRDKEA
                     AAGFFEKKFK"
     assembly_gap    7530..7629
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7630..8325)
                     /gene="truB"
                     /locus_tag="ACZPCG_05695"
     CDS             complement(<7630..8325)
                     /gene="truB"
                     /locus_tag="ACZPCG_05695"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HIR1284602.1"
                     /translation="MYHGIINVYKEPGYTSHDVVARLRGILKQKKIGHTGTLDPAAEG
                     VLPVCLGMGTRLCDMLADRTKTYEAVLLLGCSTDTQDTTGTILTENREKALALDPETA
                     EKVILGFKGDYDQIPPMYSALKVDGKKLCDLARAGKEVERKARRVQILEISILNMELP
                     RVSIRVTCSKGTYIRTLCDDIGRELGTGGCMEHLLRTRVDRFLVEDSLKLDEIEQLRD
                     MDGLEEKIYTVEEA"
     gene            complement(8326..9294)
                     /locus_tag="ACZPCG_05700"
     CDS             complement(8326..9294)
                     /locus_tag="ACZPCG_05700"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HIR1284603.1"
                     /translation="MTVLEQMLEQTGSVVILGHVRPDGDCLGSTLGLYHYIQTNYPAI
                     RAAVYLEESSPKFGYLAGYDAILHETDEERYELCICLDCGDEERLGSFGVFLANASKS
                     LCLDHHITNTRFCEVNLVSENASSTCEVLFEQLDEEKIDKAAAECLYTGLIHDTGVFK
                     YSCTSARTMEIAGKLMAKGIDFGSIIDNSFYKKTYVQNQILGRALLESITFLDGKCIF
                     SALRQSEMEFYGVNGKDMDGIIDQLRLTEGVEVAIFMYQTGPQEFKVSLRSQNAVDVS
                     RIASYFGGGGHVRAAGCTMSGRIHDVVNNLSLHIAKQLDAAGPQEE"
     gene            complement(9295..9675)
                     /gene="rbfA"
                     /locus_tag="ACZPCG_05705"
     CDS             complement(9295..9675)
                     /gene="rbfA"
                     /locus_tag="ACZPCG_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HIR1284604.1"
                     /translation="MRKNSIKNTRINMEVQRELSEIIRSGIKDPRIHPMTSVVAVEVT
                     PDLKYCKAYISVLGDEEAAKATVEGLRSASSYVRRELARRVNLRNTPEIKFILDQSIE
                     YGVNMSKLIDEVTKDLHDEDEEGL"
     gene            complement(9715..12435)
                     /gene="infB"
                     /locus_tag="ACZPCG_05710"
     CDS             complement(9715..12435)
                     /gene="infB"
                     /locus_tag="ACZPCG_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HIR1284605.1"
                     /translation="MVRAAVSQKKEAPKAEPAKKKLTAVFRPQNAQQQIKRPVPKQAP
                     KKEAPVSAASEAKAPAAARPEAQPEERTGTRENRSRDTRGEMRSQNRDGRTGAGRGEG
                     RVRDTRDGRQGGFGGNREGSRDGRSGGFGNREGNRDGRSGSFGNREGNRDGRNGSFGN
                     REGNRDGRSGSFGNREGNRDGRSGGFGNREGNRDGRNGGFGNREGNRDGRNGGFGGNR
                     DGRQGGFGNRDGRSGGFGQDKDRDSRSTGNRDSRRGNGRDAGRSSSFDSPIQTKPSST
                     RQTNKNAHKNDRFDKRDREEDGKKAPKTGKGAFIMPQPKKEEAKVEDIKTITLPERLT
                     IKELADKMKLQPSVIVKKLFLKGQMVTLNQEIDYEQAEEIAMEFDVLCEQEEKVDVIA
                     ELLKEDEEKAEDMVPRPPVVCVMGHVDHGKTSLLDAIRKTNVTAREAGGITQHIGASV
                     IEINDRKITFLDTPGHEAFTAMRMRGAQSTDIAILVVAADDGVMPQTVEAINHAKAAG
                     VEIIVAINKIDKPSANIDKVKQELSEYELIPEDWGGSTIFVPVSAHTREGVTDLLEMV
                     LLTADVLELKANPNRKGRGLVIEAELDKGKGPVATVLVQKGTLRVGETIAAGSCYGKI
                     RAMMDDRGRRVKEAGPSTPVEILGLNDVPQAGEVFVATENEKEARSFAETFISEGKNK
                     LIEDTKAKLSLDDLFSQIKAGNVKELPLIIKADVQGSVEAVKQSLTRLSNEEVVVKVI
                     HGGVGAINESDVTLASASNAIIIGFNVRPDATAKSIAEREKVDLRLYRVIYQAIEDVE
                     AAMKGMLDPVFEEKVIGHAVIRQLFKASGVGTIAGSYVLDGKFQRGCSCRITREGEQI
                     YEGPLASLKRFKDDVKEVAAGYECGLVFEKFNDLKEDDVIEAYTMVEVPR"
     gene            complement(12548..12883)
                     /locus_tag="ACZPCG_05715"
     CDS             complement(12548..12883)
                     /locus_tag="ACZPCG_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007713851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L7Ae/L30e/S12e/Gadd45 family ribosomal protein"
                     /protein_id="HIR1284606.1"
                     /translation="MVNSKKLLSLIGLATRARKVVSGEFSTEKSVKSGRSRMVIVSEE
                     ASDNTKKMFTNMCAHYKVPIYLFGTKDELGHAMGQEFRASLSVEDAGFAKSMAALMNV
                     NGGSLNESK"
     gene            complement(12870..13145)
                     /gene="rnpM"
                     /locus_tag="ACZPCG_05720"
                     /gene_synonym="ylxR"
     CDS             complement(12870..13145)
                     /gene="rnpM"
                     /locus_tag="ACZPCG_05720"
                     /gene_synonym="ylxR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003508382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase P modulator RnpM"
                     /protein_id="HIR1284607.1"
                     /translation="MSGKKIPLRQCIGCGEMKSKKEMIRVIKTAEDQILLDATGRKNG
                     RGAYLCPSMECLKKAVKSRGLERSFKMAIPKEVYEALEKEMEELGKQ"
     gene            complement(13163..14347)
                     /gene="nusA"
                     /locus_tag="ACZPCG_05725"
     CDS             complement(13163..14347)
                     /gene="nusA"
                     /locus_tag="ACZPCG_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HIR1284608.1"
                     /translation="MNKELLEAMEILEKEKNISKDTMIEAIENSLITACKNHFGKADN
                     VRVAMNRTTGDFAVYAEKEVVEEVEDSIMEISLADARMISPKYVIGDTVQVPLDSKKF
                     GRIATQNAKNVILQKIREEERKALYNDYYSMEKDVATGIVQRYLGRNISINLGKVDAI
                     LNESEQVKGETFRPTERIKVYILEVKDTTKGPRISVSRTHPDLVKRLFEEEVSEVHDG
                     TVEIKAIAREAGSRTKIAVKSNEAGVDPVGACVGVNGARVNAIVNELRGEKIDIINWD
                     ENPAYLIENALSPAKVICVVADEEAREAQVIVPDYQLSLAIGKEGQNARLAARLTGFK
                     IDIKSETQAKEMGLFEQMGLQYGDGPAPVYEEEGCGNEEEYEASGEDAAQDDYREESS
                     EQ"
     gene            complement(14369..14836)
                     /locus_tag="ACZPCG_05730"
     CDS             complement(14369..14836)
                     /locus_tag="ACZPCG_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002571156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HIR1284609.1"
                     /translation="MAKKESYESRVEAFLLPLMEENHFELVDVEYVKEAGTWYLRAYI
                     DKEGGIAVDDCEVISRQLGDWLDKEDFISESYILEVSSPGLGRPLKKEKDYVRSMGKE
                     VEVRLYKAIDRQKEFTGILSAYDDKTVTLSMEDGSTQTFEKAGIALIRLAFDF"
     gene            complement(15000..16139)
                     /gene="nagA"
                     /locus_tag="ACZPCG_05735"
     CDS             complement(15000..16139)
                     /gene="nagA"
                     /locus_tag="ACZPCG_05735"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HIR1284610.1"
                     /translation="MRIQGKKMWIAGQFMAAQLLIEEGVIRQVLPYGAKEADEDYGDN
                     RVLPGFIDIHTHGAYGYDTNDARPEGLREWMRRIPEEGVTSILPTTVTQMPDVLTAAV
                     ASVAKVVEEGYEGAQILGIHFEGPYLDMDYKGAQPPEAIAVPSVEQFQMYQEAAKGLI
                     RYITLAPEHDPDHRLTRYCASNGVAVSMGHSSASYEKALLGIANGATSMTHVYNGMTP
                     YHHRNPGLVGTALRVRDIYGEVICDGCHSHLAALNNFFTAKGRDYAIMVSDSLRAKHC
                     PPGGNYQLGGHDIEIGEDGLARLKGTSTIAGSTLSMNKGLKILIEDAMVPVDAAINSC
                     TLNPARCLGADRTKGRIAAGCDADLAVLDGDYNVIQTWCRGKKML"
     gene            complement(16301..>16639)
                     /locus_tag="ACZPCG_05740"
     CDS             complement(16301..>16639)
                     /locus_tag="ACZPCG_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004853738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIR1284611.1"
                     /translation="VFIGNINRFGPFRELAVRVISGHELAASVIMSQVISNVPAALLL
                     SNFTQQWELLIVGTNLGGLGTLIASMASLISYKQIAARYPEKKTAYLRTFTFWNVIFL
                     AALWIMASVL"
BASE COUNT         3619 a         4684 c         3580 g         4656 t
ORIGIN      
        1 actgtcattt cctcttcttc atttaaattc ttctcgtctg ccatattggg tttcctttct
       61 cagatatgat ctgtttacca cttacaatgt atcagagtct gtaaaaaatt gcaaggggtt
      121 cttaagcgga gagcgcgcat tttacagcgc tctacggttc ctaatattct atcccctccc
      181 gcgccccgat tcctctttca tagggatggc ggcggagggt catttctgtt acatagtcgg
      241 ctgcatttaa aagtgcttct gaggggccgc gtcctgtgag gatgacctcc aaagaggctg
      301 gacgggttct caaaagctcc aagaggcgtt cttcgcagat aaagccctgg cggcaggcgc
      361 cgatggcttc gtccattacc agaaggtcaa aggcaggtat tccgttttca tccgtttcca
      421 gggccatttt ccaggctttt tccatctgtt cggaataata gacagccgct ttctctttct
      481 gctccggcgt catgttccat gaaaaaccga aggagcgcac acagggaagc acatggattt
      541 cgggaatgtt ctgaagggca tttacctccc cggagtcatc tgtctttaac aggcgcagca
      601 ccagtacgcg aaagcctctg cccgcagccc ggatggcggc tcctatggcg gctgtggtct
      661 ttccttttcc atcgccgcag taaatatgga tcaaaccggt tctctcattt ttattttcca
      721 ttcaaatcct cgtttctggt ttctttcctt ctccgcttcc cgtctcgtct cagaatgtgt
      781 atccagaagc cctgtttcct ccacaaaccg cgatacagtc tgctttttat gaaacctctc
      841 gtttacataa aagatatgaa gccggtcctt tgcacgggtc atagctacat agaacattct
      901 ccgttcttct tccagatccg catccagcac tgccttgtga tggggcgtaa cgccttcatt
      961 tgcgtccaga atatacacaa ccttaaattc cagtccctta gagctgtgca tagtcattag
     1021 gctgactccc tcgcccctgg cttctcggtc tgctgcctga cggagcagct cttctttata
     1081 ttcttccatg tgagcagacc aggtgtccca tgtcttaaag cctccggcgc tttcctgaag
     1141 ggcgtccagc acttcaaaga attcctctgg ttttaaccgc cgctcctgag catattcacg
     1201 gatataatca tcgtatccaa tggccttgcg tatatagttt accgcaccgg ccggcgccat
     1261 atccgcaagc atggaaagat cgtattccag ctgttcaatc cggtccagca tccagttttt
     1321 atcctgataa aaagatttta cctgctccca gctgatcctg ctgccttcca gcgcatccct
     1381 gctgatatac cgggccgggc ggttgataaa ggtcatcacg ttggcacggc tcaaatcgcc
     1441 cctggctgct tttatgtagg aaaacaggtt ctttgcaatc cagtgttcgt agagattggg
     1501 gactgtatcc cgcatatgaa aagggatatt gtactccatg agcttttcga tcagaagtct
     1561 tggccccagg ttggtgcggt acaaaacggc aatctcttcg tatggaatcc ccatttgggc
     1621 atagtcccgc agctgggaca cgattgccag cgtttcattt accggatccg gccacagaca
     1681 gatgtccacc ggccgccctg ttcctctgag cgcgcagatt tcctttggaa aacgggtctt
     1741 attattgctg ataacctttc cggcgctttc cgtaatcgtg ctgactgagc ggtaattgat
     1801 atctaaaagg atccgctccg cttcgggata atccttctca aagcccagca tcagctccgg
     1861 ccttgcaccc ctgaagcggt agatcgactg atcgtcatct cctacaataa agaggttatt
     1921 ctgaggaaga gccagcattt ttacgatttc gtactgaacg cggttgatgt cctgaaactc
     1981 atcaatgaga atatactgat acttcttctg ccaggcagta aggatatccg gccgggcctt
     2041 aaaaagctca tagcacatgg acagcatatc gtcaaaatcc aggagctttt cccgccgcat
     2101 ctgatcctca tagccctcat acagtgcctt aaaaacctct tcggaacagc ttttgggata
     2161 ataatgcgca agaggcatca tttccccctt taccatgctg atctcgctta aaacggatgc
     2221 cgtaaattct gccggatcct ccagatccag ctgcatacgc tctgcctgct ctctaataaa
     2281 acggatcctc tgctcttccc ttacgatatt tgaagcctga tacccgtaag ccagctttaa
     2341 aatcaggaaa aacacggaat gaaaggtgcc aaaggccact ctggaaccct ggcccgtcat
     2401 tttctcatac cgttccttca tttctctggc ggctgccctg gtaaaggtaa tcaccagaat
     2461 gctcccagcc tggacaccgt atttttccgt taaataacgg acccggttgg tgatgaccgt
     2521 agtcttcccc gaaccgggtc ctgcaagtac aagagccggt ccgtctttgt ggcggatggc
     2581 ccttttctgt gcttcgctga atgacatttt atgcctccag cagttcaatg cccctgcgga
     2641 tgcgggctaa tgactcctct ttgccaagaa cctccataat ctcggtggct cctgcaggcg
     2701 tcatctgctt tccggataca gcagtgcgga taggccacat aacaaaacct gtctttacgc
     2761 ctgcatcgct gacatatttg gacaaaagct cgtaaagccc gtcattggag taatcctcct
     2821 gtgcctctaa aaggggaaga acatccttta atacctgaag ggatgtttcg ctgtttgtct
     2881 tcatcttctt atgagtatac atagctgtgt catactcagg aagagtttca aagaaatcaa
     2941 tatggccttc gatatcgggg aatacctcga ttcttgtctt taccatggca gcgatctttt
     3001 tcagatccag atcctttttg atcactctgc ggatataggg ctctgccatc tcatagaaac
     3061 ggtcaaagtc catggactta atgtattcgc cgttcatcca gcgcagcttt gtcatatcaa
     3121 atacagccgg tgacttgctc atacggcggt aatcgaactc ccggatcata tcctccaggg
     3181 agaaaatctc ctgatttccc tcaggggacc agccaagcag agctacatag tttaccacag
     3241 cctctgatac aaagccctga tcgatgagat cctcaaagga ggaatggccg ctgcgcttgc
     3301 tgagtttatg atggttctca tccgtgatca acggacagtg aacgtatacc ggaacctccc
     3361 agccaaaagc gtcatagaga cggttgtact ttggtgagga ggaaaggtac tcatttccgc
     3421 ggactacatg ggtaattccc atcagatgat cgtctactac attggcaaaa ttgtaggtgg
     3481 gatagccgtc ggacttgatc aaaaccatat catccagctc tgagttatct acagtaatat
     3541 ctccgtaaat ctcatcatgg aaggtggttg ttcctgtggt aggattgttc tggcggatta
     3601 caaatggctt tccggctgcc agattggcct cgatctcctc tttggacagg tgaaggcaat
     3661 gcttgtcata agacatgatc tcctcgccgt ttacattcgt tctcagagat tccagacgct
     3721 ccggagtaca gaagcagtaa taagcctctc ccttttcaac cagcttcttt gcatactcca
     3781 tatagatgcc ggaggcctgg cgctcactct gtacataagg gccgcagccg ccgtccttgt
     3841 cgggaccttc atcgtggatc aggccggttt ctgccatggt gcggtagata atgtctacag
     3901 ctccttctac atagcgttcc tggtctgtat cttcaattct taaaagaaaa tctccgccct
     3961 catgctttgc gatcagatag gcgtaaagcg ctgttctcaa atttcctaca tgcatacggc
     4021 ctgtagggct gggtgcatac cttgttctta ctttcatggt ctgttcctcc cgttatttta
     4081 ttagcaatca ttcatttttc tgcaaaaggc aatgcctccg ggaagcactg cctttttacg
     4141 gtccatcttt attctgccag atcgcttggt ctcatggtca ggttaatacg gcccatttta
     4201 tctacttctg ttacctttac agtaacctcg tctccgatat ttaccacatc ttcaaccttg
     4261 cccactctct tatcggacag cttggaaatg tgcaccatac cgtccttatt gggagccagc
     4321 tgtacaaagg cgccgaagtt catgatacgt acaaccttgc ctgtaaatac cataccggcc
     4381 tcaatgtccg ttacaatgct ggtgatgata gcaatggcac ggtcgatcat agcctcatcc
     4441 gtaccgcata catttacaga accctcatcg gaaatgtcaa tcttaacgcc ggtttcctcg
     4501 atgatcttgt tgattacctt gccctgcttt cctaccacat caccgatctt ctgaggatca
     4561 atggtgatct gcttgatctt tggagcatat gggcttaaat gctttctcgg ctcagaaatc
     4621 acaggcttca tcacttcctc cagaatgtac attctggcct gtcttgttct ggcaatggcc
     4681 tcctcgataa tggggcgtgt aaggccgtgg atcttaatat ccatctggat ggctgtgata
     4741 ccgttctttg taccgcctac cttaaagtcc atatcgccga agaagtcctc cagaccctgg
     4801 atatcggtga gaacgatata atcatcatcg gtctcgcctg ttaccagacc gcaggaaata
     4861 cccgctacca tctccttaat cggtacgcct gctgccatta aggacagagt agacgcacag
     4921 atacttgcct gagaggtgga accgttggac tccatggtct cagatacggt acggatcgca
     4981 tatgggaact cttcctcgct tggaagcaca ggaaccagtg ccttctcagc cagagcgccg
     5041 tgtccgattt ctcttctgcc cggtcctctg cttggctttg tctcacctac agaaaaggag
     5101 gggaagttat aatgatgcat ataacgcttg cttgtctcat ttacatccat gccgtcaatc
     5161 ttctgagcct cagataaagg cgccagggtg caggcattta agatttgagt ctgaccgcgg
     5221 gtgaacatac cggaaccgtg tactctgggc agaagatcga tctcagctgc cagcggacgg
     5281 atctgatcga tggcacggcc gtcgggacgc ttgtgatcct ttaagatcat cttgcggacg
     5341 gtctttttct catactggta aatggcgtct cctactaaag gcagccactc ttcattttct
     5401 gcaaatgcct cttccagacg ggctgtgatg gcatccatgt tcttgtcacg ggtctgcttg
     5461 tcatccgtaa atacagcctc ttccatctcc tgaggcggaa ccagctcgcg gatggcagca
     5521 aaaagctcct ccggtacggc acagctctcg tatgtgtgct tttcctttcc gcactctgcc
     5581 acaatggtat cgataaagcg gataatctcc tgatttacct catgagcctt gaaaatagcg
     5641 tcgatcactg tctgctcggg aacctcattg gcgccggcct cgatcatgat taccttttct
     5701 ctggtagatg ctacggtcag agccaggtcg gatacgcgct tctggtcagc agttgggtta
     5761 atgataaatt cgccgtcgat caagcctacc tgagtcgttg cgcaggggcc gtcaaaggga
     5821 atatcggaaa tgctggttgc aatggcagaa cccagcatgg cagtcagctc agggctgcac
     5881 tccggatcta cagacataac tacgttgtcc agagttacgt cattgcggta gtccttcgga
     5941 aacagaggac gcatggggcg gtcgatcaca cgggaggtca gaatggcatt ttcagaagcc
     6001 tttccctctc tcttattaaa tccacccgga attttgccta ctgcgtaaag cttctcctca
     6061 tattctacgc ttaaagggaa gaaatcaatg ccctctctgg gctttgagga tgccgttgcg
     6121 gtagaaagaa caacggtatc gccgtaatgc ataaatgcag cgccgtttgc ctgagctgct
     6181 actctgccga tctctacggt cagcttacgg ccggccaggt ccatactgaa acttttaaac
     6241 atggtttgtc tccttttctt ttttaatgcc aacgccaggt cttatcacag gtgcagaaag
     6301 acgccgaaac tactgatctg ggccaaagaa tcctttggaa cacatcagca atcccggagt
     6361 gaaccttctg cgtccaatcc tgttttctgg tgtaaaacag ggtggaactt gcgctccacc
     6421 ctgatgtttc ataaattact ttctgatgcc cagcttctcg atcagtgcac gatagccttc
     6481 cagatctgtc ttctttaagt agtccagaag acctcttctc tgaccaacca tcatcagcag
     6541 accgcgtctg gaatgatgat cctttggatt ctgctgcaga tgtgctgtca gctctgtgat
     6601 tctctctgtg aggatagcga tctgaacctc tggtgaacct gtgtctccag ccttacggcc
     6661 gtactctgcg ataatctgtg ccttcttttc cttagaaatc atggtaattt cctcctgtta
     6721 aaataatttt actgaatcta accgtaaacc aggctgccgg ctggagtcac cggacaacgg
     6781 ggcatcggcg tctgttcata ccgatttagt atagcatagg gtgcggggat tgtaaactct
     6841 ttttatttga attttttctc aaaaaatccc gccgcagcct ccttatcccg gctgatctgc
     6901 tcctttagtt cttccagaga atcaaatttt cgttctcctc tgataaaatc caaaagctgc
     6961 acttctatgg tgcttccgta aatatcccgg tccagaccca tgatatatgt ctcggctccg
     7021 gggggattgt cgccctctac cgtgggcttt ttcccaatat ttgtaactcc ctgataatag
     7081 acaccatcaa ccagaactct cgaaacgtaa acgccgtacc tgggaaggcg cttaatggcg
     7141 ggagggatca gattggcggt tggaaatccc aggaccgcgc ctccgatatg attcccgtga
     7201 accaccttcc catggatggc atagggttct cccaaaagct cattggcctt ggctacgttt
     7261 ccgcaggcca gctcctcccg gatgtaggtg ctgcttatat ccctgtgccg gtcttcatcc
     7321 tgctcttttt caatgacata aagactgtag ccgcaggagg ctgccagctt tcttaaaagc
     7381 tctgcattcc ctgctccctg atagccgaag ctgcagtccg gacctactac aatggcagcc
     7441 gccttcatcc ggccggacag gatctgaaca acaaaatcct ccggggccat tcgccgcacc
     7501 tgatcgtcaa atggatattc caccagatan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnc gcctcctcca ccgtgtaaat tttctcctca aggccatcca tgtctctcag
     7681 ctgttcgatc tcatccagct tcagactgtc ctccactaaa aaacgatcca ctcttgtcct
     7741 gagaagatgc tccatacatc cccctgtgcc cagttctctt ccgatatcgt cgcacaaggt
     7801 ccgtatgtag gtgcctttgg agcaggtaac ccggatggat actctgggaa gctccatatt
     7861 taagatggaa atctccagaa tctgtactct tctggccttc cgctccactt cctttcctgc
     7921 acgggccagg tcgcagagct ttttcccgtc cacctttaag gcggaataca tgggggggat
     7981 ctggtcataa tctcccttaa atcccagaat cactttctct gccgtctccg gatccagagc
     8041 cagggctttt tcccggttct ctgtaaggat cgttcctgta gtatcctgcg tatctgtgga
     8101 gcagcctaaa agaagcactg cctcgtaggt ctttgtccgg tctgccagca tatcgcacag
     8161 tcttgtgccc attcctaaac ataccggaag cacaccttct gccgccggat ccagcgttcc
     8221 ggtgtggccg atttttttct gctttaaaat ccctctgagc cgggccacca catcgtggga
     8281 tgtgtagccc ggctccttat atacattgat aatcccgtgg tacatttatt cctcctgagg
     8341 gccggctgca tctaactgct tggcgatgtg gagcgacaga ttattgacta cgtcatggat
     8401 tcttccagac atggtacagc ccgctgcacg cacatggccg ccgccgccga agtaggatgc
     8461 gatcctgctt acatctaccg cattctggga acggaggctt accttaaatt cctgagggcc
     8521 ggtctggtac atgaaaatgg ctacctccac gccttcggta agacgcagct ggtcaataat
     8581 gccgtccata tcctttccat ttaccccata aaactccatt tccgactgac gcagagcact
     8641 gaaaatgcat tttccgtcca aaaaggtaat gctttccaga agagcacggc ccagaatctg
     8701 gttctggaca taggtctttt tataaaagct gttatcgata atagagccga agtcaatccc
     8761 ttttgccatc agctttcccg cgatttccat ggtacgggcg ctggtacagg agtatttaaa
     8821 tactcctgta tcatggatca ggcctgtata caggcactcg gccgcagctt tatcaatctt
     8881 ttcctcatcc agctgttcaa acagaacctc gcaggtagag cttgcatttt cagataccag
     8941 atttacctcg cagaagcggg tattggtaat atggtgatcc agacacagac tctttgacgc
     9001 attggcaaga aaaacgccaa aggatcccag ccgttcttca tctccacagt ccagacagat
     9061 gcaaagctca tagcgttcct catctgtttc atggaggatc gcatcatagc ctgcgagata
     9121 tccaaacttc ggtgagcttt cttccagata taccgctgcc cggatggccg gataattggt
     9181 ctgaatgtag tgataaagtc ccagagtcga acccagacag tcgccgtccg gccttacatg
     9241 gcctaaaata acgaccgatc cggtttgctc cagcatctgt tccagaaccg tcatttacag
     9301 tccctcctca tcctcgtcat ggagatcctt tgtcacttca tcaatcagtt ttgacatatt
     9361 tacgccatat tcaatggact ggtccagaat aaatttgatt tccggcgtat tgcgcaggtt
     9421 cactcttctt gcaagctctc tgcgcacata ggaagaggcg ctgcgaaggc cctcaacggt
     9481 cgccttcgct gcttcctcgt cgcccagaac actgatatat gccttgcagt attttaaatc
     9541 aggggtcact tccacggcaa ccacactggt catgggatgg atgcgcggat ccttaattcc
     9601 ggagcggatg atctcgctta attccctctg aacctccatg ttaatccttg tgttcttgat
     9661 gctgttttta cgcatttata tttcctttcc ggtatgcatt acccgaaacg gctcttacct
     9721 tggcacctct accatggtgt aagcctcgat tacgtcatct tccttaaggt cgttgaattt
     9781 ctcaaataca agaccgcact catagcctgc cgctacttcc ttaacgtcat ccttgaaacg
     9841 ctttaaggat gctaacggtc cttcgtaaat ctgctctccc tcgcgggtaa tacggcagct
     9901 gcagcctctc tggaactttc cgtccagcac ataggagccc gcgatggttc ccacgccgga
     9961 agccttgaac agctggcgga ttaccgcatg gccgatgacc ttttcttcaa atacagggtc
    10021 taacataccc ttcatagctg cctctacgtc ctcaatggcc tggtagataa ccctgtagag
    10081 acgaagatct accttttcac gctctgcaat ggatttagct gtggcatcag gccgcacatt
    10141 gaagccaatg atgatcgcat tggaagcaga tgccagagtt acgtcggact cgttgatcgc
    10201 acctacgccg ccatggatta cctttaccac tacctcttcg tttgagagtc ttgtaaggct
    10261 ctgctttaca gcttctacgg aaccctgtac gtctgccttg atgatcagag gcagttcctt
    10321 tacgtttcct gccttgatct ggctgaacag atcgtccaga gacagcttcg ccttcgtatc
    10381 ctcgatgagc ttgttcttgc cctcggaaat gaaggtctct gcaaagcttc ttgcctcttt
    10441 ttcattttca gttgcaacaa atacttctcc ggcctgagga acgtcattta aaccgaggat
    10501 ctcaaccggt gtggaaggcc ctgcttcctt tacacggcgt cctctgtcgt ccatcatggc
    10561 acggatcttt ccgtaacagg aaccggcggc aatggtctcg cctacacgaa gggttccctt
    10621 ctgtaccagc accgtagcaa caggaccctt gcccttatcc agctccgcct cgatcacaag
    10681 gcctcttccc ttacggtttg gatttgcctt taactccaga acgtctgctg tcagaagcac
    10741 catttccaga agatccgtta cgccctctct ggtatgtgcg gaaaccggca cgaaaatcgt
    10801 gctgccgccc cagtcctcag ggatcagctc atattcagac agctcctgct ttactttatc
    10861 aatattggcg cttggcttat cgatcttgtt gatggcaaca atgatctcaa ctcctgccgc
    10921 ttttgcatgg ttaatggcct ctacggtctg tggcatcacg ccgtcgtctg ccgctacaac
    10981 caggatagcg atatccgtag actgagcgcc tctcatacgc atggcggtaa acgcctcatg
    11041 tcccggtgta tcaaggaagg tgatctttct gtcattgatc tcgatcacgg aagcaccgat
    11101 atgctgggtg atgccgcctg cctctcttgc agttacattt gtcttgcgga ttgcatccag
    11161 aagagaggtt ttaccatggt caacatggcc cataacgcag actaccggag gtctgggaac
    11221 catatcttcc gccttctcct cgtcttcctt taagagttct gcgatcacat ctaccttttc
    11281 ttcctgctca cagagtacat caaactccat tgcaatctct tcagcctgct catagtcaat
    11341 ttcctggttt aaggtaacca tctggccctt taagaacagc ttctttacga taacagacgg
    11401 ctgaagcttc atcttatctg ccagctcctt gatagttaag cgctcaggca gagtgatcgt
    11461 cttaatatcc tccaccttgg cctcttcctt cttaggctgc ggcataatga aagctccctt
    11521 tcctgtcttt ggtgccttct tgccgtcctc ttcccggtct ctcttgtcga aacggtcatt
    11581 cttatgagcg ttcttattgg tctgacgggt actgcttggc tttgtctgga tcgggctgtc
    11641 aaaggaagaa gaacgtcctg cgtctcttcc gtttcctctg cggctgtcac ggtttcccgt
    11701 acttctggaa tctctgtcct tatcctgacc aaaaccgccg cttcttccgt cacggtttcc
    11761 aaaaccgccc tgacggccgt ctctgtttcc tccgaaaccg ccgtttcttc cgtcacggtt
    11821 tccttctctg tttccgaaac cgccgtttct tccgtcacgg tttccttctc tgtttccgaa
    11881 accgccgctt cttccatcgc ggttcccttc tctgtttccg aagctgccgc ttcttccatc
    11941 acggtttcct tctctgtttc cgaagctgcc gtttcttccg tcacggtttc cttctctgtt
    12001 tccgaagctg ccgcttcttc cgtcacggtt tccttctctg tttccgaagc cgccgcttct
    12061 tccatcacgg cttccttctc tgtttcctcc aaaaccgccc tgacggccgt ctcttgtatc
    12121 gcggactctt ccttcgcctc tgccggctcc agtgcgtccg tcacggttct ggctgcgcat
    12181 ctcgcctctt gtatcacggc tgcggttctc tcttgtcccg gttctctcct caggctgagc
    12241 ctctggtctc gctgctgcag gagcctttgc ttcggaagct gctgagacag gagcttcctt
    12301 tttcggagcc tgctttggaa caggacgctt tatctgctgc tgtgcgttct gtggacggaa
    12361 tacagcggtc agcttcttct ttgcaggttc tgcctttgga gcttcctttt tctggctcac
    12421 tgccgcccgc accatggctt cctggtcgcc ggataagctg gatgcagcaa acagagaggc
    12481 agactgatcc ttctgcttca ctatatctaa tacctcttta ctggttttgc ccagttcatt
    12541 tgccagttca cttactttca ttcaaactac ctccatttac attcatcagt gctgccattg
    12601 actttgcgaa ccctgcatcc tccacagaga gagaagcgcg aaactcctgc cccatggcat
    12661 gaccgagttc atcctttgta ccaaacagat agatcgggac tttatagtga gcacacatat
    12721 ttgtgaacat tttctttgtg ttgtccgaag cttcctctga tacgatcacc atgcgggatc
    12781 tgccgctttt aacggacttt tccgttgaaa actctccgct gaccaccttt ctggccctgg
    12841 ttgcaaggcc gatcaggctc agcagctttt tactgtttac caagttcttc catctccttt
    12901 tccagagctt catatacctc tttggggatt gccattttaa aggagcgttc cagcccgcgg
    12961 ctctttacgg cctttttcag acattccatg gatggacaca ggtaagcgcc ccggccgttt
    13021 ttgcggcctg tggcatccaa caggatctgg tcctcagcgg tttttatcac tcttatcatt
    13081 tctttcttgc tcttcatctc accgcagccg atacactgcc tcagagggat cttctttcca
    13141 ctcactctct atcactccaa tcctactgtt cgctgctctc ttcccggtag tcatcctgag
    13201 cggcgtcttc cccggaagct tcgtactcct cttcgtttcc gcagccttct tcctcataaa
    13261 caggagccgg accgtcgccg tactgtaagc ccatctgctc gaagagtccc atttcctttg
    13321 cctgagtctc gctcttgata tcaatcttaa agcctgtcag tctggctgcc agtctggcgt
    13381 tctgtccctc tttaccgatt gctaaggaca gctgatagtc aggcacgatc acctgagcct
    13441 ctcttgcctc ttcatctgcc actacgcaga taacctttgc agggcttaag gcattttcga
    13501 tcagataggc cggattttcg tcccagttga taatgtcgat cttttcgccc ctcagctcat
    13561 ttacaatggc attgactctg gctccgttta cacctacgca tgcgcctaca ggatcgaccc
    13621 cggcctcgtt ggacttaacg gcgatttttg tacgggagcc tgcctcgcgg gcgattgcct
    13681 tgatctctac agtaccgtca tgaacctcgg atacttcctc ctcaaacaga cgctttacca
    13741 gatccggatg ggttctggat acagagatcc tggggccctt ggtggtgtcc ttcacctcaa
    13801 ggatatatac cttgatacgc tctgtgggac ggaaggtttc tccctttacc tgctcgctct
    13861 catttaaaat cgcatctact ttgcccagat tgatgctgat gtttctgccc aggtaacgct
    13921 gtacaatacc cgtagcaaca tccttttcca tgctgtaata gtcattgtaa agagccttgc
    13981 gctcctcctc gcggatcttc tgtaagatta cattctttgc attctgtgtg gcaatacggc
    14041 caaacttctt ggaatcaagc ggtacctgta ccgtatcccc gatcacatac ttggggctga
    14101 tcatacgggc atctgccaga ctgatctcca taatggaatc ctctacctct tccacaactt
    14161 ccttctccgc atatacggca aagtcccctg tggtccggtt catcgccact cttacgttgt
    14221 cagcttttcc gaaatgattc ttacaggcgg taataaggga attttcgatc gcctcgatca
    14281 tggtatcctt agagatattt ttctctttct ccaatatctc catcgcctct aacagttcct
    14341 tgttcatgat tatcctccta tctatgcctt agaaatcaaa cgccagccgg atcagcgcga
    14401 tccccgcctt ttcaaatgtc tgggtacttc cgtcctccat ggacagagtc accgtcttat
    14461 catcatatgc gcttaaaata ccggtaaatt ccttctgccg gtcgatggcc ttgtaaagac
    14521 gaacctctac ttccttgccc atactgcgca catagtcctt ttcctttttt aaaggacgtc
    14581 ccagtccggg agaacttact tccagaatgt agctttcgga aataaaatcc tctttgtcca
    14641 gccagtcccc cagctgtctg ctgatgacct cacagtcatc tacggcaatg ccgccttcct
    14701 tgtcaatgta cgcccgcaga taccaggttc ccgcttcctt tacatattcc acgtctacca
    14761 gctcaaaatg attctcttcc atcagcggaa ggaggaatgc ttccactctg ctctcatagc
    14821 tttccttctt tgccatcatt ccaccacctt tcttttccct tacaatacga aagagtggac
    14881 cgtcgcccac tcttattatc agtaaaacta tctgttatca tttacactat accatcagac
    14941 cagaggaaat gcaagccctt ttttgcgaaa acagccgtaa cttccggtat tttctcagcc
    15001 tacagcatct tctttccccg gcaccaggtc tggatcacat tgtaatcccc atcaagtacc
    15061 gccaggtctg catcacagcc tgctgcgatc cgtcccttgg tgcggtcagc gcccagacag
    15121 cgggccggat ttaaggtgca ggaattgatg gctgcatcca ccggaaccat ggcatcttcg
    15181 atcaggatct taagcccctt attcatgctc agggtgcttc ccgcaatagt agaagtaccc
    15241 tttaaccggg ccagtccgtc ctcaccgatt tcaatgtcat ggcctcctaa ctggtaattg
    15301 cctcccggag gacaatgctt ggcccggaga gagtcgctga ccataatggc ataatcgcgc
    15361 cctttagccg taaagaaatt gtttaacgca gccagatggg aatgacagcc gtcacagatc
    15421 acctcgccgt aaatatcacg aacccgaagc gccgttccca ccagtcccgg attacggtga
    15481 tggtagggcg tcataccgtt gtatacatga gtcatggagg tggctccgtt ggcaattccc
    15541 aaaagggcct tctcatagga agcagaggaa tggcccatac ttactgccac gccgtttgaa
    15601 gcacagtacc gggtcagtct gtgatccgga tcatgctccg gcgcaagggt aatatagcgt
    15661 atcaggccct tagccgcctc ctgatacatc tgaaactgct ctacagaagg cacggcaatg
    15721 gcttccggag gctgggcgcc cttgtaatcc atgtccagat aggggccttc aaaatggatc
    15781 cccaaaatct gcgcaccctc gtatccctct tccactacct ttgccacact ggctaccgca
    15841 gccgtaagaa catccggcat ctgggtcact gtcgttggga gaatcgaggt tacgccctcc
    15901 tccgggatcc tcctcatcca ctctctaagc ccctccggtc tggcgtcgtt tgtatcatat
    15961 ccgtaagcac cgtgggtgtg aatatcaata aagccaggaa gcacgcggtt atccccgtaa
    16021 tcctcatccg cctctttcgc cccgtaagga agaacctggc ggatcactcc ttcctcaatc
    16081 aaaagctgtg ctgccataaa ctgacctgca atccacatct tttttccctg aatacgcatc
    16141 gttatctcct tttccgattg tctgcctcaa aataaggcgt aaatctttcc tgttatatca
    16201 accgttaaat gataccatca gtataccaca tgagaattat ttttcaagtt tgtgatataa
    16261 gcgaaatatt tttcttttgc cgtttttatc cataatgcag tcacaaaacg gaagccatga
    16321 tccacaaagc agccagaaag atcacattcc agaatgtaaa cgtcctcaga tacgccgttt
    16381 tcttttccgg atatcgggca gcaatctgct tataggagat cagacttgcc atagaagcga
    16441 tcagcgttcc caagccgccc agattcgtcc ccacaattaa aagctcccat tgctgcgtaa
    16501 aattggacaa aagcagagct gccggcacat tgctgattac ctggctcatt atgacagagg
    16561 ctgccagctc atgacctgag atcactctta ccgccagttc gcgaaaaggg ccaaaccggt
    16621 ttatatttcc aataaacac
//
LOCUS       DBMDUH010000046        16062 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_2055_length_16062_cov_4.644007, whole genome shotgun sequence.
ACCESSION   DBMDUH010000046 DBMDUH010000000
VERSION     DBMDUH010000046.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 16062)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 16062)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16062
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2055_length_16062_cov_4.644007"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..2061
                     /locus_tag="ACZPCG_05745"
     CDS             <1..2061
                     /locus_tag="ACZPCG_05745"
                     /inference="COORDINATES: protein motif:HMM:NF013126.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HIR1284612.1"
                     /translation="NAGGEGVGRYTNAETLYPADGTKCGNLGENFAYDQVSYVDYAEG
                     IYVGYRWYETADAQHFWDGVENRYGRGYDGVVQYPFGYGLSYTEFQWEFLEAPKEQTV
                     PDPFGEISVMVRVTNTGKRAGQDVIQLYVSPPYTPGGIEKSSVALAAYAKTSLLEPGA
                     CETLTLTVPVSSFASYDEKDANKNGFQGYELEAGIYELSLRSDAHHPAAMDQNTIRLY
                     VPAGTLYDTDRITGAPVFNKFTGEQAQDGVSIDGSDAGGAIHYLSRADFNKTFPKANV
                     PVRRLSHKGAALNLYTKELADTWHSDETWTASDEALRDKKSVKKLTLMVERMGSLTDL
                     GYGLGKNWEDPRWEEVLDQLTFSDMENLFLHGYVKTEPLSVLGKPLAREADGPAQIGS
                     FHQIPAGTGFPNAGILAQTWNNALAREMGRAIAKEAGQFGYSGWYAPAVNLHRSPFDG
                     RNYEYYSEDPLISGHMGGLVVRGSLDMGVYCYVKHLICNDQESGIYRDGIYTWMTEQT
                     LRELYLKPFQMLVQDYGATGFMTAYNRIGAVWAGGSQALLTGILKEEWGFHGAIVTDY
                     ADHHKYMNGDQALRAGGSLWMDGILNDGKFRFLTEENEAFYYHALRRTSKEVLYMYLN
                     ARAENREYSQRMGDPGLLKPVIYPAFPLWGAALGAFDLMAASAFLFCLRRTKKPEKKK
                     KRRE"
     gene            2066..3046
                     /locus_tag="ACZPCG_05750"
     CDS             2066..3046
                     /locus_tag="ACZPCG_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009251151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR1284613.1"
                     /translation="MEETKNHKDTTLSRLPVVICLAVVCTFLWGSATPAIKIGYEQFH
                     IEGMDSWSIIVFAGLRFLLAGLMVIGFYCIQKKRLILPPAGAGSCILKLCLCQTVLQY
                     YFFYMGLVYVTGVHGAIITGTNVFLSILIACLLFGYEQLTGRKLLGCLFGLAGIVLVN
                     LSGQTSEGLFDVSFRGEGFVFIAQIFYALSGALVKRYSKQYDVVVLSGYQFTMGGIIL
                     ILIGLGCGGRVDLMAGAEAVVLLLYLGLISAVAYTLWSILLKYNPVSRVTVFGFMIPV
                     FGVLLSAVFLGESSQALSWNVLAALILVSLGIFVVNTSEKPEKKDGTEGK"
     gene            3049..3495
                     /locus_tag="ACZPCG_05755"
     CDS             3049..3495
                     /locus_tag="ACZPCG_05755"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1284614.1"
                     /translation="MEEIINITENKKKYLELLLLGDEKEEMIDRYLERGDMYVLKTPL
                     GMALCVVTDEGDGVLELKNIAVEPVCQKKGYGRRLIRFICQKYKNQFHTLQAGTGDSP
                     LTVPFYEHCGFIRGSVIPHFFMDHYDHPIYEGGKQLDDMIYFQLKL"
     gene            3520..4143
                     /locus_tag="ACZPCG_05760"
     CDS             3520..4143
                     /locus_tag="ACZPCG_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016298246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4317 family protein"
                     /protein_id="HIR1284615.1"
                     /translation="MIDRNDMLELTRRLTPERSSVGRIAGAYFDAEGYVDGTFNINFL
                     KLSVPERKKNLSIAKTVLFSETNSLLKEYRLTKEARRPGGLWQLLSAIKECGMKNDAY
                     TDLFYEILGEKYRPGGPWSCFLFHGRYDVPVKGLDKAWMEGSEEVYEYLICAISPLTG
                     EYEPGEPEFGFLYPAFKERGAALNFVNLFEKDPNRPHRELTEWFLKG"
     gene            4293..4883
                     /locus_tag="ACZPCG_05765"
     CDS             4293..4883
                     /locus_tag="ACZPCG_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284616.1"
                     /translation="MAKNSQKHHVESISYRSRLAYEEMLNIILNCTERKTFDLSGING
                     GHTSGTRKLGLDYAELIRDYCAITEYGNYIVKLSVFDDQGNLVQTGSSFGSTDDAQGI
                     IESGWLDRELEKTMDRYQLDLESSPFSQYEGNILPIARKLEHSGSGGWAVLCLSTDLF
                     KDMLLDTANGQETVIVTAAGDRIASIEEPGGAQRGK"
     gene            4840..5931
                     /locus_tag="ACZPCG_05770"
     CDS             4840..5931
                     /locus_tag="ACZPCG_05770"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1284617.1"
                     /translation="MLLLKNRGEHREENDRIIRSLQERKKDKGLLEMKVHGKDSLVTY
                     EIYDKSGIMVYETMPLDELSNDRMMIVQTVVIMFLACLIFGLLLSARITKQIKKPIDR
                     LTAHIETIAAGNFIRDDSIEGEDEIGRVGCVVNDMALRIKELMDQQLKAEKEKGDLEL
                     KMLQAQINPHFLYNTLDSIRWIAVIQKNSGIVKMVTALSGLLKNMTKGFNEKVTLKKE
                     LEFLNDYVTIEKVKYVELFDLEIQVDDPGLYEAKVIKLTLQPLVENAIFNGIEPKGRH
                     GIIKIRASRQEDMLVLTVRDNGVGIPKERLKTLLENTEKVKGNSMSGIGLPNVDRRIK
                     LNYGESYSLMVDSTEGKFTEITVTMPLEF"
     gene            5959..7563
                     /locus_tag="ACZPCG_05775"
                     /pseudo
     CDS             5959..7563
                     /locus_tag="ACZPCG_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007713664.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            7774..9075
                     /locus_tag="ACZPCG_05780"
     CDS             7774..9075
                     /locus_tag="ACZPCG_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1284618.1"
                     /translation="MTASMAAGCGSSSSTADSKAADTTAAGESTEEKKEEGASDGKKT
                     LSISVWDNDATPQFTAVAEAFMAANPDVTIEFIDAPSSEYNNKVTVMLAGGDSEPDVI
                     FVKDTENQVSMKEKGQILNLDEYIKKDAIDLGMYQGVAEQLQMDGSNYTLPFRKDWYM
                     LFYNKDLFDKAGVEYPSADMTWDEYEELAKQMTSGEGSSKVYGTHNHTWQALVSNWAV
                     QDGKNTLMSEDYSFMKPAYEQAVRMQDEGIMQSYANLKTGSIHYISVFEQQQCAMLPM
                     GSWFIGTLIQDKEAGKFDFNWGVTTIPHPEGVEAGATVGSTTPVAINAKSDDPDLAWE
                     FVKFATGEEGAMALADNGIFPAASTDAINEKLAAIPGFPEDGTDALNITSWVLDRPID
                     AKMAAVRKVLEEEHDLIMIGEEDIDTGIANMNQRAKEARED"
     gene            9188..9950
                     /locus_tag="ACZPCG_05785"
                     /pseudo
     CDS             9188..9950
                     /locus_tag="ACZPCG_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574010.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            9963..10790
                     /locus_tag="ACZPCG_05790"
     CDS             9963..10790
                     /locus_tag="ACZPCG_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006781481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1284619.1"
                     /translation="METRKNVNRIFIYGLLTVLAILVVIPFGWMLLASVKTSNEVFSI
                     PMRLLPKEFQWVNYQTIWEKIPLLTFFKNTFKIAICTTAVQVLTSCFAAYGFSKVQFK
                     GRDTLFLISVTTFAVPWQAYMVTQFALISRMHLTDSHLGLILMQAFSGFGVFLIRQFM
                     IGVPNELLEAARIDGLNEYGIFARVALPLVKPGIATLVIFTFVNIWNDFMGPLIYLNS
                     TELKTIQLGIRMFISQYGADYALIMAASVCSLIPVLIVFMVCQKWFVEGITAGGVKG"
     gene            10795..12795
                     /locus_tag="ACZPCG_05795"
     CDS             10795..12795
                     /locus_tag="ACZPCG_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HIR1284620.1"
                     /translation="MTGEVMKEQAGYCKSRWADQCSHSIQTAEDVLNRKFLFDLPWDM
                     EQTTEPVIFTDQIDWQYMPGGDPEFIYQFNRHRYWICMGQAYALTGNEAYAACFKEQL
                     LSWISENPITESTKKTTWRTIEAGIRGENWCKAIEYFESSPHMGDEEKQAFIKGLLLH
                     GEYLFNCRVPFSDKSNWGVLENHGLFCIGAFLSKKEWAIEESSRKKGALYIEEALKRL
                     KRELAIQVMDDGVHWEQSPMYHNEVLRCILEVLRVAELYEIPVAEDIEKAARAMAMAD
                     LLWSKPDHTQPANGDSDRTDLRDVLTPAAWLLKDPLLRFGGYDCLDFESAWEMGMEAV
                     IGYEQMTKETPQNELVSLEASGQAVLRSGWDQKADYLHFMCGSLGGGHGHFDKLHLDL
                     SYEGEDVLIDTGRYTYVDGTLRRTLKSAKAHNVPMADDREYTECTGSWNVRNAAAYAG
                     MRVVQKGQYTLFEGAHLGYMDAGVYVCRKVLSIGTKIQIIADSFYGFGEHVCSQHFHL
                     NPAGQTTLTKEDQASGLGFSYQGIKTGAKAFVLMPGAEISMEQGPVSFHYNQLTECPW
                     ICIRKKMALPGTLITVIFNDNTADASLVPVTVPVTGEVLKDQDAQALRIRMGDHSYLA
                     VFNHRETGADMEYIGADGHYGLGRVMICEENQPEPEMTVLSW"
     gene            12932..13744
                     /locus_tag="ACZPCG_05800"
                     /pseudo
     CDS             12932..13744
                     /locus_tag="ACZPCG_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002571225.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
     gene            complement(13741..15216)
                     /locus_tag="ACZPCG_05805"
     CDS             complement(13741..15216)
                     /locus_tag="ACZPCG_05805"
                     /inference="COORDINATES: protein motif:HMM:NF039231.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HIR1284621.1"
                     /translation="MKKYKSFIPTLGAALLLSLGSAFCAQAADIGWVTEDGTWRYKDA
                     SGNYVTNTWKTSGDSSFYLGSDGKMTVNQWIDDEYYVNDSGAMVKNSWIHITEEGGSK
                     PAGWYYTDSKGKLERDGWETIGTYKYAFDSDGRMRTGWFFDGDDIYYLGGENQGYAKT
                     GWQCLDYDEEDKPEDGDISEARSSASDSSKWFYFQSNGKAKRADDRTYAVETINDRKY
                     YFNEDGVMMTGWIAAEEEAEAGDTTGISRFVYLGDENDGTMARDTWLELTEHPASCDD
                     KDELAEGDTDEMPEDGDSNWYYFESDGTPAYLNAKASSMSRATTKVNGDSYFFNPYGV
                     RQTGMIRMVNQSVEEMVGYFGDSNSDGKMVTGKKTNLNVDGDSGTYYFADSGSDKGAG
                     LNGTKDGYLYYQGRLVEAEDGSDFEVFEVKNRLYLVNESGKVQTDSKNYKVDGSYMYK
                     ISGGTIYYIDDDKNVEGKVEASDASTLPEVIYDKEYVLNGN"
     gene            15410..>16062
                     /locus_tag="ACZPCG_05810"
     CDS             15410..>16062
                     /locus_tag="ACZPCG_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR1284622.1"
                     /translation="MERGPIRAAIISDTHGVLRPEVERILETCDVIVHAGDFDTQMLY
                     HKLEVNQPLFAVRGNNDRGWSGGLPGIKRFEIEGVKFIMAHERTQIPGSLGDAQVVVF
                     GHSHMYQQQEINGRLWLNPGSCGYKRGTLPLSMAVMTIENGAYTLETIWLEKGYGTPE
                     DAIAKREKPKKNRYERQQKRHMQEKKDKEMLFLIAKILRLYQADASRAWVVKNTGNDP
                     "
BASE COUNT         4376 a         3358 c         4209 g         4119 t
ORIGIN      
        1 aatgcaggag gagagggtgt gggacgctat accaacgcag agacgctcta tcctgcagat
       61 ggcacgaaat gcggcaatct gggagaaaat tttgcctatg atcaggtttc ctatgtagac
      121 tacgcagagg gaatctatgt gggctatcga tggtatgaga cagcagatgc acagcatttc
      181 tgggatgggg tagaaaacag atatgggcgg ggctatgatg gagtggttca gtatccgttt
      241 ggatacggcc tttcctatac ggaatttcaa tgggaatttt tggaggctcc aaaggaacag
      301 acggttccgg atccattcgg agaaatttct gttatggtcc gtgtcacgaa tacgggaaaa
      361 cgggccggtc aggacgtgat ccagctgtat gtgtcgcctc cgtatactcc ggggggaatt
      421 gagaaatcat ccgttgccct ggcagcctat gcaaaaacat cgctccttga gccgggagcc
      481 tgtgaaactc ttacgcttac cgttcctgtt tcttcctttg cttcctatga tgaaaaggat
      541 gcaaataaaa acggatttca gggttatgaa ctggaggcag gcatatatga gctgtctctg
      601 cgctcggatg cccaccatcc ggcagccatg gatcaaaata ccatccgtct gtatgtaccg
      661 gctggaacgc tttatgatac agaccggatc acaggtgctc ctgttttcaa taaatttacg
      721 ggggaacagg ctcaggacgg tgtttccata gacggttctg atgcaggagg agcgatccat
      781 tatctgagcc gggctgattt taataaaacc tttccaaaag ccaatgtgcc ggtacgccgt
      841 ctttctcata aaggggcggc cctgaatctg tataccaaag aactggcgga tacctggcat
      901 tcagatgaga catggactgc ttcagatgaa gcgttacggg acaaaaagtc tgtaaaaaag
      961 cttacgctta tggtagaacg aatgggaagc cttacagatc tgggatatgg gcttggaaaa
     1021 aactgggaag atcccagatg ggaagaggta ctggatcagc ttaccttttc tgatatggaa
     1081 aatctctttc tgcatggcta tgtaaagact gagccgttat ctgtactggg aaaaccctta
     1141 gccagagagg cagacggacc agcccagatc ggaagctttc accagattcc ggcaggaact
     1201 ggcttcccca atgccggcat actggctcag acctggaata acgcattggc ccgggaaatg
     1261 ggaagagcaa ttgcaaaaga agcaggccag tttggttaca gcggctggta tgctcctgcg
     1321 gtgaaccttc accgcagccc ctttgacgga agaaattatg aatattattc ggaggatccc
     1381 ttgatctcag gccatatggg tggtcttgta gtgaggggaa gcctggatat gggcgtatat
     1441 tgctatgtaa agcatttgat ctgcaatgat caggaatcgg gaatttaccg ggacggcatt
     1501 tatacctgga tgacagagca gacgctcagg gaactgtatc taaagccctt ccagatgctg
     1561 gttcaggatt atggagcaac tggctttatg acagcctata accggatcgg agcggtgtgg
     1621 gccgggggga gccaggccct tttaaccgga atccttaaag aggaatgggg ctttcatgga
     1681 gccattgtca cagattatgc ggatcaccat aaatatatga acggggatca ggctctgcgg
     1741 gccggaggaa gcctgtggat ggatgggatt ttaaatgatg gaaaattccg cttcctgacg
     1801 gaggaaaatg aggctttcta ttatcatgct cttcgcagaa cctccaaaga ggtgctctat
     1861 atgtatttga atgccagagc ggaaaacaga gagtacagcc agaggatggg agaccccggt
     1921 cttttaaagc cggtgattta tcctgccttt cctctgtggg gagccgcatt aggcgctttt
     1981 gatcttatgg cggcatctgc ctttcttttc tgtctcagac ggacaaagaa accagaaaaa
     2041 aagaagaaaa gaagagagtg aaaaaatgga agagacaaaa aatcataaag atacaaccct
     2101 gtcccggctt ccggtggtga tctgcctggc tgtcgtctgt acctttttgt ggggaagcgc
     2161 cactccagcc ataaagatcg gctatgaaca attccacatc gaaggtatgg attcatggag
     2221 tattatcgtg tttgccggac ttcgcttttt gcttgccgga ttgatggtga tcggctttta
     2281 ctgtatacaa aaaaagaggc tgatcctgcc tccggcggga gcaggaagct gcattttaaa
     2341 gctctgccta tgtcagacgg tgctccagta ttacttcttt tatatggggc tggtttatgt
     2401 aacaggggtt catggggcga tcataacagg taccaatgta tttctttcaa tcctgatcgc
     2461 ctgccttctg tttggatatg aacagcttac aggaagaaag ctgcttggct gcttgtttgg
     2521 actggcaggc attgtattgg tcaacctttc cggacagaca tccgaagggc tgtttgatgt
     2581 atccttccgg ggagaaggct ttgtatttat tgcacagatt ttttatgctt tatccggcgc
     2641 attggtaaaa cgctactcca aacagtatga cgtagttgtt ttaagcggat atcagtttac
     2701 catgggaggg atcattttga tcttgatcgg acttggctgc ggaggaagag ttgatcttat
     2761 ggcaggagct gaagctgtgg ttcttcttct gtatttgggg ctgatctctg ctgtggccta
     2821 tacgctttgg agtattcttt taaaatacaa tccggtcagc cgtgtaactg tttttggctt
     2881 tatgatcccg gtctttggag ttttgctgtc agccgttttt ttaggggaaa gcagccaggc
     2941 tttatcatgg aatgtactgg cagcgctgat tttggtaagt cttggaattt ttgttgtaaa
     3001 tacatcggaa aagccggaaa aaaaagacgg aacggaagga aaataaattt ggaagagata
     3061 ataaatatta cagaaaataa aaagaagtat ttagagcttt tgcttctggg ggatgagaaa
     3121 gaagaaatga ttgaccggta tctggaacgc ggagatatgt atgttttaaa aacgccctta
     3181 gggatggctc tctgtgtagt gacagatgag ggagatgggg tattagagct taaaaacatc
     3241 gcggtggagc cggtgtgcca gaaaaaggga tatgggcgca ggctgatccg ttttatctgt
     3301 cagaaatata aaaatcagtt ccataccctt caggcaggaa cgggagacag ccctcttaca
     3361 gtgccgtttt acgagcactg cggctttata cggggatctg tgattccgca ttttttcatg
     3421 gatcactatg accatccgat ttatgaggga ggaaaacagc tggacgacat gatttacttc
     3481 cagcttaagc tttaaaaaca aaggggagga agaaacagga tgattgaccg aaatgatatg
     3541 ctggagctga cccgcaggct aacgccggag cgttcttctg tgggccggat tgccggagcg
     3601 tactttgatg cagagggcta tgtggacggt acctttaata tcaatttctt aaagctttcc
     3661 gtaccggaga ggaaaaagaa cctttccatc gccaagactg tgcttttttc tgagacaaat
     3721 tctctgctaa aggaatatag gcttaccaaa gaagccagac ggccgggagg attgtggcag
     3781 cttttaagcg ccattaagga atgcggcatg aaaaatgacg cctatacgga tctgttttat
     3841 gagattttag gagagaaata ccgtccgggc ggtccatggt cctgcttcct ttttcatggc
     3901 cgatacgatg tgccggtaaa agggctggat aaagcgtgga tggaaggttc tgaagaggtg
     3961 tacgaatacc tgatctgcgc tatcagccct ttaaccggag aatatgagcc gggagagccg
     4021 gagtttggct ttttataccc ggcttttaaa gagcggggcg ccgccctgaa ttttgtaaat
     4081 ctctttgaaa aggatccgaa ccggccccac agggagctta cagagtggtt tttaaaggga
     4141 tgaaaatgaa aaaggaaaca aaaccatgaa aaatatatcc gaaccaaaac actgcgcgtt
     4201 cagttgatgc gctatcattt ctgatcatat gcctgattgc aacctttgta agtatctata
     4261 cctacataac tgcgaaccag aaaactctgg aggtggcaaa aaattctcag aaacaccatg
     4321 tagaaagtat ttcctaccga agccggctgg catatgagga aatgctcaat attatcttaa
     4381 actgtacaga gcggaaaacc tttgatttgt ccggaataaa cggaggacat acctcaggaa
     4441 ccagaaaact cggactggat tatgcggagc ttataagaga ttactgtgcg attacagagt
     4501 acggaaacta tattgtaaag ctgtctgtct ttgatgatca gggaaatctg gttcagacag
     4561 gctcttcctt tggaagcaca gatgacgcgc aggggatcat agagagcggc tggctggatc
     4621 gggagcttga aaaaaccatg gaccgttatc agctggatct ggaatcatct ccattttccc
     4681 agtatgaggg aaatatcctt ccaattgcca ggaagctgga gcattctggc tccggcggct
     4741 gggcggtgct gtgtctttct acggatctct ttaaggatat gcttttggac acggcaaacg
     4801 gacaggaaac ggtgatcgta actgcggccg gagaccggat tgcttctatt gaagaaccgg
     4861 ggggagcaca gagaggaaaa tgaccggatt atccgcagcc ttcaggaacg gaaaaaggat
     4921 aaaggactac tggaaatgaa ggttcatggg aaagacagtc tggtaacata tgagatttat
     4981 gataaaagcg ggatcatggt atatgaaacc atgcccttag atgagctttc caatgaccgg
     5041 atgatgattg tacagactgt agtgatcatg tttttggcct gtttgatttt tggacttctg
     5101 ttatccgcgc ggattacaaa gcagataaaa aagcctattg accgtctgac tgctcacatt
     5161 gaaaccattg cggcgggaaa ttttatcagg gacgattcga tagaaggcga ggatgaaatc
     5221 ggccgtgtag gctgtgtggt aaatgatatg gctctgcgga tcaaagaact gatggatcag
     5281 cagctgaagg cagaaaaaga aaagggagat ttagaactta agatgctcca ggcccagatc
     5341 aatcctcatt ttctttacaa cacgctggat tccatccgct ggatcgcagt gatccagaaa
     5401 aacagcggca ttgtaaaaat ggtgaccgcc ttatcaggcc ttcttaaaaa catgacaaaa
     5461 ggctttaatg aaaaagtgac tctgaaaaaa gaactggaat ttttaaatga ctatgtaacc
     5521 atcgaaaagg taaagtatgt ggagctgttt gatctggaaa tacaggtgga tgatcccggg
     5581 ctttatgaag caaaggtgat taagctgaca ctgcagcctc tggtggaaaa tgctattttc
     5641 aacgggattg aaccaaaggg ccgtcatgga attatcaaaa tccgggcatc cagacaagag
     5701 gatatgctgg ttcttaccgt gcgggataac ggtgtgggga ttccaaagga acggttaaaa
     5761 acacttcttg agaatacaga aaaagtaaag ggaaattcca tgagcgggat cgggcttccc
     5821 aacgtagacc gccggatcaa gttaaattat ggggagtcct atagcctgat ggttgacagc
     5881 acagagggaa aatttacgga aattaccgtt accatgccgc tggaatttta aaatataaat
     5941 gcaacagggg gaagaggaat gtatcagatt atgattgtgg atgatgagcc gctgattctt
     6001 gcaggaatcg cctctctttt agactgggaa aagcaggagt gtgtgatcgc cggaaaggca
     6061 gggaacggac aacaggcctt aaagctgatg gaggaacaga gaccggatat tgtgatcaca
     6121 gatattaaga tgccgggaat ggatggaatc tcctttatga aggctgtaaa ggaacggggc
     6181 tgggatgata ttgtttttat cattctcacc aatctggagg aatttaacct ggctcgtcag
     6241 gcgctgtctt taggagccgt agaatatctg gtaaaaatgg aactgacaga ggagaaactt
     6301 tcagaggcct taaagcttgc aaaagagcgc tgcgaattga cacggcttgc aaatgaaaaa
     6361 gcgtccccgg gctttccctc caaagcagac cggatccgcc gtttgatcga gtagcttctg
     6421 gtcggagacg agcccgcaga agggtttaag gaggcagagg agacgcttaa ggagccagtg
     6481 cttgtgatta tcagcttcaa ttacgggttt gagggttttt cggagggatt tacccgagaa
     6541 gaccagaaaa aagtgatttc ctttgcagaa aatattatcg gtcagatgac aggaggttat
     6601 ttcgacagca gctgtctggt gtgccgggag ctggacagcc tggtgcttgt actgtctgct
     6661 gcaggagtgg aaaattatca gacggtcatc cgaagtctgg gcgaaaaaat ccggtctgtg
     6721 gtaaaggatt actttgaggt gccggtatcc gtggcagtca gcaccagaaa ggaaagcctg
     6781 cgtgaatttc cggccctgct ctatgaggcc atgagcgcta ccagctatta tttcggccat
     6841 tccggagatc cggtagtctt ttattccaga cagtgtgaaa cagatgcggg tcacccggga
     6901 agctttcata tcggcctcct gaaaaaagat ctgtcccagg cgatcgcctt aaatgacagc
     6961 agccgcttaa ggcaggtaat cggtcagttg acgcagcttt tggaggaata tcacccttca
     7021 agggaacagg ctgtgaatgc ctgtgccaat ctgtattttt ttatagcatc tttttttgag
     7081 gatgatgaag aaggcggttt tccctacgag gtcaacatta tggaaaagct ggggcgtttt
     7141 ggcacactgg aacagatttt gaaatggatc cgctggtttg aggcttctat atctgctatt
     7201 ttggatcgga gaagagacag ccgcacggat aagattgcgg aaatggtccg ggattatgta
     7261 atgacccatt ataagaaacg gatcactctg ggacagacag ctgaagccct gggtatcagc
     7321 caggggtatt taagcacggc ttttaaaaag cagacagggg aatcctttac aggattcgtg
     7381 tcagccgtaa agattgaaaa ggccaaggaa ctgatcgccg gccatcagta tatgatgtat
     7441 gagatttccg atatgctggg ctttgatacg cctttttatt tcagtaaggt atttaaaaag
     7501 atcgccggtc tttctccgaa ggaatatgaa gcagaatgtt tcaaggccgg ccatgaggtg
     7561 taaaagggaa aaagtgtaaa aaagtgcaat aatataaaaa aagtataaaa agaaataggc
     7621 ctgtcctgaa atttttataa aaagtcttta tttgttgaat gggctttgga agctgttttt
     7681 gttatagtat acacagaaag caaagaggta ttattttaag gagggcttta caatgagatt
     7741 aaaaaggcag cagcgcttac actggtaacc gctatgacag cttctatggc agcaggatgc
     7801 ggcagcagct catccacagc tgattccaag gcggcagata ccacagcggc aggagagagc
     7861 acggaggaga aaaaagaaga gggagcttcc gatgggaaga agaccttaag catttccgta
     7921 tgggataatg atgcaactcc tcagtttacc gcagtggcag aggcatttat ggcagccaat
     7981 ccggatgtga ccatcgagtt tatcgatgct ccgtcaagtg agtataacaa caaggtaacg
     8041 gttatgctgg caggcggcga ttccgagccg gatgttatct ttgtaaagga tacagagaat
     8101 caggtatcca tgaaggaaaa gggacagatc ttaaatctgg atgagtacat taagaaagat
     8161 gccattgatc ttggaatgta ccagggcgta gcagagcagc ttcagatgga cggaagcaat
     8221 tacacccttc cattccgcaa agactggtat atgctgttct acaacaagga tctttttgat
     8281 aaggcaggcg ttgagtatcc atccgctgat atgacctggg atgagtatga agaactggca
     8341 aagcagatga caagcggcga gggcagcagc aaggtatacg gaactcataa ccatacctgg
     8401 caggcacttg tttctaactg ggccgtacag gatggaaaga acaccctgat gagcgaggat
     8461 tacagcttta tgaagccggc ttatgagcag gccgtacgga tgcaggatga aggcattatg
     8521 cagtcttatg cgaacttaaa gacaggaagc attcattata tcagcgtatt tgagcagcag
     8581 cagtgcgcaa tgcttcctat gggttcctgg tttatcggta ctctgatcca ggataaagag
     8641 gcaggaaagt tcgactttaa ctggggcgta accaccattc ctcatccgga aggcgtagag
     8701 gcaggagcta ctgtaggatc tactacaccc gttgctatca acgcaaagtc agatgatccg
     8761 gatcttgcat gggagtttgt aaaatttgca accggtgagg agggcgcaat ggctctggct
     8821 gacaacggta tcttcccggc tgcatctacc gatgcgatca atgagaagct ggcagcaatt
     8881 cccggtttcc ctgaggatgg aaccgatgct ttaaatatca caagctgggt acttgaccgt
     8941 ccaatcgatg ccaagatggc agcagtaaga aaggttcttg aggaagagca cgacctgatc
     9001 atgatcggtg aagaggatat tgatacaggt attgcaaaca tgaaccagag agcaaaggaa
     9061 gcaagagagg actaatcctc attttggatc caaagccata atacaaaacg taagagcgtc
     9121 tgatttcagt cttcagcgaa agttggacgc tctttttgta cccttatata aggacaggtg
     9181 agaaaaaatg acacaaaaac agaaaaaaat attaaagagg aacctgatcg gatattcttt
     9241 tatcctcccc aattttatcg gatattttct ctttattttc cttccggtga tctttacatt
     9301 cgttctctgt gtgatgaaat gggacggttc ttctcattgc aattctgtta aattccaaga
     9361 tcaagggaat tgtgatttac cgtaccgcct tctttttccc ttatgtggca tctttggtgg
     9421 ctgtaggtgc ggtctggaat atgctgttcc agccggagtt tggaccgatc aatgaatttc
     9481 taaaatggat cgggatcgtc aacccgccga aatggtgcgc ttctaccgac tgggccatgt
     9541 ccgttattat tatcgtcagc gtgtggaaat acatgggcta ttacatggtc gtttatctgg
     9601 ccgcacttca gggcatttcc ggagatcttt atgaggcagc cagcattgac ggagcaaccg
     9661 gctttaagaa gttccgctat attacccttc ccatgttaaa gccaacgact ttctttgtta
     9721 tcattatgat gacgatccag tgctttaggg tatttgacct gatctatgtt atgacaggcg
     9781 gaggccctgg acgtgcaacc aatgtgcttg taaaccatat ttacaatgca gcctttgttg
     9841 actttaagtt cggttatgcc agcgccagcg ctcttgtgct gtttgctgtt gttctggtgg
     9901 ttacgctggt acagttccag ggtgaaaaga aattcaccga ttatgtataa ggaggaaaga
     9961 tcatggaaac aagaaaaaac gttaaccgca tatttatcta tggtcttctg actgtacttg
    10021 ccattttagt ggtaataccg ttcggatgga tgctccttgc atctgtaaag accagcaacg
    10081 aggtgttcag catacccatg cgtctgctcc ccaaggaatt tcagtgggtc aactatcaga
    10141 ccatctggga gaagatcccg cttcttacct tctttaaaaa tacatttaaa attgccatct
    10201 gcaccacagc cgttcaggtt ctcaccagct gttttgcagc ctacggcttt tcaaaggttc
    10261 agtttaaggg aagagatacc ctgtttttaa tttctgttac cacctttgca gtaccctggc
    10321 aggcatacat ggtaacgcag tttgcactga tttcccgtat gcatctgacc gactcccatc
    10381 tgggattgat cctgatgcag gcattctccg gttttggcgt attcctgatc cgtcagttta
    10441 tgatcggtgt accaaatgaa cttttggagg cagccagaat tgatggctta aacgaatacg
    10501 gaatttttgc aagagtggcg cttcctctgg taaagcctgg aattgcaacc ctggttattt
    10561 ttacatttgt aaatatctgg aatgacttta tggggccctt gatctattta aacagtacag
    10621 agttaaagac catccagtta ggtatccgta tgtttatttc ccagtacggc gcagattatg
    10681 cactgatcat ggctgcttca gtctgctctc tgatccctgt gctcatcgta tttatggtat
    10741 gccagaagtg gttcgtggaa ggcatcacag ccggcggcgt aaaggggtga tcctatgaca
    10801 ggagaagtga tgaaggagca ggccggatac tgtaagagcc gctgggctga tcagtgcagc
    10861 cacagtatac agactgcaga ggatgtttta aatcggaaat tcctctttga tctgccctgg
    10921 gatatggagc agaccacgga gcctgtgatt tttacagatc agattgactg gcagtatatg
    10981 cccggaggcg atcctgagtt tatctatcag tttaaccgtc accgctattg gatctgtatg
    11041 ggtcaggcct atgcgcttac aggaaatgaa gcctatgcgg cctgctttaa agagcagctt
    11101 ttatcctgga tttcggaaaa tccgattaca gagtctacaa aaaagactac ctggagaacc
    11161 attgaagccg gtatccgcgg agaaaactgg tgcaaagcca ttgagtattt tgaatccagt
    11221 ccccatatgg gagatgagga gaaacaggcc tttataaagg gcctgctcct tcatggggaa
    11281 tatcttttta actgccgggt tccgttcagc gataagagca actggggcgt tttggaaaat
    11341 cacggacttt tctgtatcgg agccttcctt tcaaaaaagg aatgggcgat tgaggaaagc
    11401 agccggaaaa agggcgctct ttatatcgaa gaggctttaa aaaggcttaa gcgggagctg
    11461 gcaatccagg taatggacga cggagtccac tgggaacagt ctcccatgta ccataatgag
    11521 gttctacgtt gtattctgga ggttttaagg gtcgcagagc tgtatgagat tccggtggcg
    11581 gaggatattg aaaaggcggc cagagcgatg gccatggcag atcttttgtg gagcaagccg
    11641 gatcataccc agcctgcaaa cggggacagc gaccggacag atcttcggga tgtgcttaca
    11701 ccggccgcat ggctgcttaa ggatccgctt cttcgctttg gaggttatga ctgtctggat
    11761 tttgaaagtg cctgggaaat gggaatggag gctgtgatcg gctatgaaca gatgacgaag
    11821 gaaactcccc aaaatgagct ggtttctctg gaggccagcg gacaggccgt tctgcgttcc
    11881 ggatgggatc agaaggcaga ttaccttcat ttcatgtgcg gaagcctggg aggcggccac
    11941 ggccactttg acaagctcca tctggatctg tcttatgagg gagaggacgt gttgatcgat
    12001 acaggacgtt atacctatgt ggatggaact ctgcgcagga cattaaagtc tgcaaaggct
    12061 cacaatgtcc ctatggcaga tgacagagaa tatacagagt gtactggctc ctggaatgta
    12121 agaaatgccg cagcttatgc cggaatgaga gtggtgcaga aagggcagta tacactgttt
    12181 gagggagccc atctgggata tatggatgcg ggagtctatg tatgccgaaa agttctgtcg
    12241 atcggtacaa agattcagat aatcgcagac agtttttacg gttttgggga gcatgtgtgc
    12301 agtcagcact tccacttaaa tccagccgga cagacaacgc ttacaaagga agaccaggca
    12361 tctggtttgg gattttccta tcagggaatc aaaacaggag caaaggcgtt tgttctgatg
    12421 ccgggagcag aaatctctat ggagcagggg ccggtatcct tccactataa ccagcttacg
    12481 gaatgtccct ggatctgcat ccgaaaaaag atggctcttc caggcactct gattacagtc
    12541 atttttaatg acaatacagc agacgccagc ctggttccgg tgaccgttcc agtaacagga
    12601 gaagtgttaa aggatcagga tgctcaggcc ctgcgtatca gaatgggaga ccattcctat
    12661 ctggccgtat tcaatcatcg ggagacaggc gccgatatgg aatatatcgg agcagacgga
    12721 cattacggcc tgggccgggt gatgatctgt gaggaaaacc agccggaacc tgaaatgacg
    12781 gttctttcct ggtaaaactg cccgattggg agcagaatca tataacaaat ggagaaaaat
    12841 tatgcagaca gcggaacgct tagaacaata tgaaagaatt acaggccggg aagcagaaga
    12901 ttttctggcc agggcagtga aactggtaca ggaaaatgca aaaaaagccg cccttatggc
    12961 agcggataat ctgatggaac ggtttcagga aaagggaagg ttctttcagg cctggggtga
    13021 actgggagca aaggacaatt accgtcttat cattgactgt cttttaaata tgcctttgct
    13081 gttttgggcc agtgaggtga caggagatcc aaagtacaga gaaaaggcta cggctcacat
    13141 tctcacagcc atgaactgtg tgatccgccc ggatcattcc acctaccata cctatttctt
    13201 tgatcctgag acgggagagc ctgtaaaggg tgtgactcat cagggcaacc gcgacggttc
    13261 cgcatggagc agaggacagg cctggggaat ttacggcagt gctttaagct atcatttctt
    13321 aaaggatcct gcatatattg atattttcag aaaagttaca gattattttg taacgcatct
    13381 gccttccgat ctgatcccct actgggattt tgattttgat gacggcagta cagaacccag
    13441 agattcctcc tccgcagcaa tcgcagcctg cggtatgctg gagatggcaa aatatctgga
    13501 gcctgaggag gcagaaaaat atacagccct ggcagaaaaa ctgataaagg ccctttgcga
    13561 tcactgcgcc gtaaaggatc acagtgagtc aaacggcctt cttcttcacg gaacctatgc
    13621 cagagccagc aagggaaatc cctgtaccaa ccggggggtg gatgagtgca atacctgggg
    13681 agattatttc ttcatggaag ccctcacccg tctgaccgcc gactggaagg tatactggta
    13741 ttagtttccg tttaaaacgt attccttatc ataaatgact tctggaaggg tagaggcatc
    13801 agatgcttct acctttcctt ctacattctt atcatcatca atatagtaga tagtgccgcc
    13861 ggaaatttta tacatatagg aaccgtctac cttataattt ttgctgtccg tctgaacctt
    13921 tcccgattca tttacaaggt acaggcggtt ttttacctca aagacttcaa aatcagagcc
    13981 gtcttcggcc tctaccagcc gtccctgata gtaaagatag ccgtccttgg tgccatttag
    14041 accggctccc ttatcagaac cggagtctgc aaagtaatag gtgccgctgt ctccgtctac
    14101 attcagattg gtcttctttc cggtcaccat ttttccgtcg gagttactgt ctccgaaata
    14161 gcctaccatt tcttccacgg actggtttac catgcggatc ataccggtct ggcgcacgcc
    14221 gtaagggtta aagaaatagg aatccccatt cacctttgta gtagcccggc tcatgctgga
    14281 ggcttttgca tttaaatagg ccggcgttcc gtcgctttca aaatagtacc agttggaatc
    14341 tccgtcttct ggcatttcgt ccgtatctcc ttctgcaagc tcatccttgt catcacagct
    14401 tgcaggatgc tccgtcagct ccagccaggt atctcttgcc atggtaccgt cattttcatc
    14461 gcccagatag acaaagcggc tgatccctgt cgtgtctcct gcctcggcct cctcctcagc
    14521 agcgatccag cctgtcatca taacgccgtc ctcattaaaa taatatttcc ggtcattgat
    14581 ggtttcgact gcgtaggtac ggtcatctgc cctttttgcc ttgccgttgg actgaaagta
    14641 aaaccatttg gaggaatcgg aggcagagct gcgtgcttcg gaaatgtcgc catcttcggg
    14701 cttgtcctct tcatcgtaat ccagacactg ccagccggtt tttgcatagc cctggttctc
    14761 gccgcccaga tagtagatat catccccgtc aaaaaaccag cccgtacgca tccggccgtc
    14821 gctgtcaaag gcatatttat atgttccaat ggtttcccag ccgtcacgct caagctttcc
    14881 cttagaatcc gtatagtacc agcccgcagg ctttgagccg ccctcctcgg tgatgtggat
    14941 ccaggaattc ttgaccatgg cgccggaatc atttacatag tattcatcgt cgatccactg
    15001 gttcaccgtc atctttccgt cgctccccag ataaaaggaa gagtcgccgg aggttttcca
    15061 ggtatttgtc acatagtttc cggaagcatc tttataacgc caggtgccgt cctccgtgac
    15121 ccagccgata tccgctgcct gagcgcagaa tgcagaaccc aaagagagaa gcagggctgc
    15181 tccgagggta ggaatgaatg acttgtattt tttcatgatc aaaggttctc cttctggtaa
    15241 ttttttgaaa tttacaggtc tattgtatca tatagaaaag ttccacacaa taaagttcac
    15301 aaaattgtca gaaaatgtgc tgttttttgt tatcctttca ttgaaaaaat ggaagctttc
    15361 ctttataatc ataaatcgtg ggacgacaca gccctaaagg aggagacaaa tggaacgggg
    15421 acccatacgg gcggctatta tatcagatac ccacggcgtt ttaaggccgg aggtggaacg
    15481 gattttagag acctgcgatg tgatcgtaca tgcaggagat tttgatacgc agatgcttta
    15541 tcacaagctg gaggtaaacc agcccctttt tgcggtgagg ggaaacaatg accgcggctg
    15601 gtccggaggc cttccgggga ttaagcgttt tgagatcgaa ggcgtaaaat tcatcatggc
    15661 tcacgaaagg acacagattc cgggcagcct gggagacgct caggtggttg tttttggcca
    15721 ttcccatatg taccagcagc aggagataaa tggacggctc tggctcaatc caggaagctg
    15781 cggctataaa cgtggtaccc tgcccctttc catggcggtg atgaccatag aaaacggagc
    15841 ctatacgctg gaaaccatat ggctggaaaa gggctacgga acgcccgaag atgccatcgc
    15901 aaaacgggaa aaaccgaaga aaaacaggta tgagcgtcag cagaagcgtc atatgcagga
    15961 gaaaaaggat aaggaaatgc tgtttttgat cgcaaagatc ctgcgtcttt atcaggcaga
    16021 tgcttccaga gcctgggttg ttaaaaatac aggaaatgac cc
//
LOCUS       DBMDUH010000047        15831 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_2079_length_15831_cov_7.402734, whole genome shotgun sequence.
ACCESSION   DBMDUH010000047 DBMDUH010000000
VERSION     DBMDUH010000047.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 15831)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 15831)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15831
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2079_length_15831_cov_7.402734"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="ACZPCG_05815"
     CDS             <1..897
                     /locus_tag="ACZPCG_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003524358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HIR1284623.1"
                     /translation="ESELLDAFFVFIKRFKTLIHFNGDRFDIPYLEKCCRRLGPSHSF
                     EGMESMDIYKKIRPYRKLLGLESMKQKAIEQFLGISREDKYTGGQLIEVYRDYLHTHE
                     KSLYDLLILHNEDDLKGMPAILPILSYADFFSSPLFPQAPELWEGDCTEPTDTPLLHL
                     MGTGSVSVPVPLEYSSGPVSLELYENKAVCTIRLCRGCLKYFYPDYKDYYYLPFEDTA
                     VHKSVGEYVDRSARKKATARTCYTKKEGLFLPQFGPFKEPSFREDYKSPLTYVLYDDS
                     LFSQPQAADTYFSQLFCWLLEQ"
     gene            complement(909..1520)
                     /locus_tag="ACZPCG_05820"
     CDS             complement(909..1520)
                     /locus_tag="ACZPCG_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284624.1"
                     /translation="MKIRGKKMALAARTVTAFRQRRFSYEDWLIFCFGAGIIGGTAVV
                     LLFGEGALGRGLLAGEGREDLWGKGMISCLTETLKDGGLEQAMKEAGVVSLLAKRLLQ
                     MTAGWVAGLTICSPFFFGCITCWGGMCLSGALAFLTLEKGVWGLPVFLWLLFPQGVIY
                     GITWAVLAGWAGGREHRLHLAAFFLLLLFTAAGALLEGIFHHP"
     gene            complement(1756..2337)
                     /locus_tag="ACZPCG_05825"
     CDS             complement(1756..2337)
                     /locus_tag="ACZPCG_05825"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR1284625.1"
                     /translation="MAQKPENEGMPVIRLDRQLKYQGNILKIYEDTVLANGHEAHWDY
                     IHHDGAAAVLPVTDEGKILMVRQYRNALDRFTLEIPAGKLDDPKEPKIECAYRELEEE
                     TGFKTEKLEYLISVNTTVAFCDEAIDIFVAHNLVPSHQHLDEDEVIEVEAWELKDLEE
                     LIFTGKITDGKTIAAIMAYGRKYRSVDKKDEFV"
     gene            complement(2351..3148)
                     /gene="proC"
                     /locus_tag="ACZPCG_05830"
     CDS             complement(2351..3148)
                     /gene="proC"
                     /locus_tag="ACZPCG_05830"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002593241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HIR1284626.1"
                     /translation="MAEIGFIGMGNMGLAILHGLLKTCEPGRFIFTAAHAEKMAAVTE
                     ATGVPHAESNRVCAQSCKYLILAVKPQYFDVVFDEIREVLEEDQVVISLAPGQTISSL
                     KERLGDEIRVVRAMPNTPAMLGAGMTGIAFGTDPFTEEEKEQICAVFKSCGGVELIEE
                     SQMEAVGCVSGCSPAFVYMFIEALADSGVKYGLPRKTACEMAAQTVLGSAKMVLETGK
                     HPGQLKDEVCSPGGTTIAGVSALEEFGFRNAVIKAADACYEKSQKMK"
     gene            complement(3161..4429)
                     /locus_tag="ACZPCG_05835"
     CDS             complement(3161..4429)
                     /locus_tag="ACZPCG_05835"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002593240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HIR1284627.1"
                     /translation="MEKKPFVTKEQLEEITKTYPTPFHLYDEKGIRENARQLKEAFSW
                     NKGYKEYFAVKATPNPFILNILKEYGCGTDCSSMTELMMSKACGFSGNDIMFSSNDTP
                     LEEFAYAAKLGAIINLDDITHIECVEQTLGYIPETISCRFNPGGVFKISNDIMDNPGD
                     SKYGMTTEQIFEAFKILKAKGAKNFGIHAFLASNTVTNEYYPMLAKILFELAVRLHEE
                     TGAHISFINLSGGIGIPYRPDQEPNDIRAIGEGVRQVYEEVLVPAGMGDVALCTELGR
                     FMLGPYGCLVTKAIHEKHTHKEYVGVDACAVNLMRPAMYGAYHHITVMGKENAPCDHM
                     YDIVGSLCENNDKFAIDRMLPEIEKGDLLVIHDTGAHGFAMGYNYNGKLRSAELLLKE
                     DGSAEMIRRAETPKDYFATFDFCNILKDAE"
     gene            complement(4545..5981)
                     /gene="pyk"
                     /locus_tag="ACZPCG_05840"
     CDS             complement(4545..5981)
                     /gene="pyk"
                     /locus_tag="ACZPCG_05840"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HIR1284628.1"
                     /translation="MKKTKIICTMGPNTNDKNVMLGLAKNGMDVARFNFSHGDYAEHL
                     GRLEILKEVRKELDIPVAALLDTKGPEIRTGILKDGKKVTLKEGQIYTLTTREIVGDD
                     TIGHINYSGLNEDVSAGNTILIDDGLIELKVNEVNDTDIVCTVVNGGELGERKGVNVP
                     NVSIKLPALTEKDKEDIRFGIKQGFDFIAASFVRTADCIREIKAMLDEAGSSIKVIAK
                     IENAEGIENLDEIIAEADGIMVARGDMGVEIPAQKVPNIQKMIIRKCNVACKTVITAT
                     QMLDSMIRNPRPTRAEVTDVANAVYDGTDAVMLSGETAMGKYPVEALKMMSSIVEETE
                     SYLDYTAYRQRKVSAENMKNVSNAVCCASVSTAHDIGADYIIVPSITGFTSMMLSKWK
                     PAARILGMSPSMSTVRQMQLQWGVTPVWSRRADSTDELIENSIEELKEKNFVESGKLA
                     VLTAGVVSYARRHEEAAQTNIMRVVEIN"
     gene            complement(6088..6942)
                     /locus_tag="ACZPCG_05845"
     CDS             complement(6088..6942)
                     /locus_tag="ACZPCG_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284629.1"
                     /translation="MNHFVNNAKRSLRRMMGGSIIDIVCGLQILGVVTYLLSPEFFGY
                     LILNPALVVRGQIWRIVTFLAYPPAVTGGDAISFVIFNAIGIYCVRLFGLLVEQVWGR
                     FKFNCYIISGVLLHAAVSVLIYLITGMPLPLFPNYLVYSFFFVFALMFPDMQVLFMML
                     IPLKSSWIAVFEGVMYIYSFVRGSLIDRIEILLSIALMGIFFGWLTFGKKGPVYQKKR
                     SREFQKKTEKIRVVKNTHRCAVCGRTSEDSPGMEFRYCSKCEGSYEYCMDHLYTHKHV
                     KKGEKPEE"
     gene            complement(6959..7900)
                     /locus_tag="ACZPCG_05850"
     CDS             complement(6959..7900)
                     /locus_tag="ACZPCG_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008719300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HIR1284630.1"
                     /translation="MIRIFKTEDGTIHEKQEMQPGSWIALTNPTASEIIDVADACRID
                     PDHLKAPLDEEERSRIEAEDEYTLILVDIPSIEERNGKDWFVTIPMAIITTRDVLITV
                     CLEETPILSAFMDGRVRDFHTFMKTRFILQILYKNATQYLQYLRIIDKKSEVIENKLH
                     QAQKNEELIELLELQKSLVYFTTSLRSNEVVLERLLKLDKIKKYPEDTDLLEDVIIEN
                     KQAMEMTSIYNGILSGTMDTFASVISNNLNIVMKFLATVTIVLSIPTMVASFYGMNVL
                     PKGMPFADSPYGFWIVLGFAVALSLIVAYIFNKKDLF"
     gene            8244..12518
                     /locus_tag="ACZPCG_05855"
     CDS             8244..12518
                     /locus_tag="ACZPCG_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008753632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HIR1284631.1"
                     /translation="MNQMSTTYRLGVDIGSTTVKVAVLDQENRILFSDYERHYANIQE
                     TLAKLLKKAVSQTGDILVIPVITGSGGLTLSSHLHIPFVQEVVAVASSLQDYAPQTDV
                     AIELGGEDAKIIYFTGGIDQRMNGICAGGTGSFIDQMASLLQTDASGLNEYAKNYQAI
                     YPIAARCGVFAKSDIQPLINDGATHEDLAASIFQAVVNQTISGLACGKPIRGNVAFLG
                     GPLHFLPELRKAFVRTLHLTPEQTIAPEHSHLFAAVGAAMNPKEDQTPLSIADLIKTL
                     SSGVQMDFEVKRMEPLFKNQEEYDAFLADHAHNHVRSSDLSSYEGLCYLGIDAGSTTT
                     KVALVGEDGSLLYRFYENNNGSPLAAAIQAAREINEQMTDKAKIAWSCSTGYGEALLK
                     AAFLLDEGEVETISHYYAAAFFDPEVDCILDIGGQDMKCIRIKDGTVDSVQLNEACSS
                     GCGSFIETFAKSLNYSVADFAKEALFAKNPIDLGTRCTVFMNSNVKQAQKEGATVADI
                     SAGLAYSVIKNALFKVIKITNASDLGSHVVVQGGTFYNDAVLRSFERISGCRAVRPDI
                     AGIMGAFGAALIARERYHLGGEKETSTMALDKIISLKYTTSMARCKGCNNHCVLTVNQ
                     FGSSRRYISGNRCERGLGMEKAKKEIPNLFDYKYRRMFDYEPLPMDEADRGCVGIPRV
                     LNMYENFPFWAVFFRKLRYHVILSPQSTKQIYELGIESIPSESECYPAKLVHGHVSWL
                     IRQGVKFIFYPCIPYERNESPEAGNHYNCPMVTSYAENIKNNVEALEEEQVRFMNPFM
                     AFTNEKILTDALVKEFSSSFQIPAAEIRMAAHAAWQELTASREDIKRKGEETLKWMKE
                     HGRRGIVLAGRPYHVDPEIHHGIPDVITSYGFAVLTEDSVSHLGKVERPLVVTDQWMY
                     HSRLYAAASFVKTQDNLDLIQLNSFGCGLDAVTTDQVSDILTRSGKLYTVLKIDEVNN
                     LGSARIRIRSLIAAIRVRDQRHYERKIISSAYNRVVFTKEMKKDYTLLCPQMSPIHFD
                     LVEPAIRAFGYNIEILQNHNKNAVDVGLRYVNNDACYPSLIVIGQIMDALLSGKYDLN
                     RTAIFMTQTGGGCRASNYIGFIRRALEKAGMSQIPVVSINANGMETNPGFSITLPLLT
                     KAMQAVVYGDIFMRVLYATRPYEAVPGSADALHEKWKKRCIASLSKRSAAMMEFGRNI
                     KGIIRDFDRLERRDIKKPKVGIVGEILVKFSPLANNHIVELLEAEGAEAVMPDLMDFL
                     LYCFYNNNFKAEHLGKKKSTARLCNAGIALLEYFRRTARKELAASRHFAPPAAINELA
                     DMAKGFVSLGNQTGEGWFLTGEMLELIHSGVKNIVCTQPFGCLPNHIVGKGVIKELRR
                     NYPDSNIIAVDYDPGASEVNQLNRIKLMLATAHKNLKKEMEPKVQV"
     gene            complement(12592..13842)
                     /gene="glyA"
                     /locus_tag="ACZPCG_05860"
     CDS             complement(12592..13842)
                     /gene="glyA"
                     /locus_tag="ACZPCG_05860"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009429759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HIR1284632.1"
                     /translation="MVNTVMDFITGYDKEVGEAIQAECRRQRQNLELIASENIVSEPV
                     MMAMGTVLTNKYAEGYSGKRYYGGCQCVDVVEDLAIERAKKLFGCDYANVQPHSGAQA
                     NMAVFVAMLKPGDTVMGMNLDHGGHLTHGSPVNFSGLYFNIVPYGVDDQGFIDYDEVE
                     RKARESKPKLIVAGASAYARTIDFKRFREIADEVGAYLMVDMAHIAGLVATGEHPSPI
                     PYADVVTTTTHKTLRGPRGGMILANKEAAEKFNFNKAIFPGTQGGPLEHVIAAKAVCL
                     GEALKPEFKEYAHQVVLNAKALAEALKKQGFKLLTDGTDNHLMLVDLRGLDISGKELQ
                     NRCDEVCITLNKNTVPNDPRSPFVTSGVRIGTPAVTTRGLKEEDMEKIAECIWLTATD
                     FENKKEYIKAEVAKLCDKYPLYEG"
     gene            complement(13952..15676)
                     /gene="recJ"
                     /locus_tag="ACZPCG_05865"
     CDS             complement(13952..15676)
                     /gene="recJ"
                     /locus_tag="ACZPCG_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009532223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HIR1284633.1"
                     /translation="MTGSIWMLHAKKADFNALSGRFHISPVTARIIRNRDIESEAEFD
                     RYLNGSLEDLYSPGLLPDMEEAVQRICRAVQEKRRLRIVGDYDIDGVCSTAILYKGFK
                     RIGACADYVIPERIRDGYGINEHIIEAAAADGIQTIVTCDNGIAAISQIALAKELGLE
                     VIITDHHDIQTNEETGEDLMPPADVIVNPKRRDSRYPWPEICGAMVVFKLVGALYEAY
                     GISRKEWLELLELAAIATVGDVMKLKDENRIVVKEGLKRLAVTGNPGLKSLIYKNALD
                     PANISAYHIGFVLGPCLNASGRLQTARLAMELLLCEDTARADSIAEELKELNDRRKAM
                     TQENVDAAALQVETELKDDKVLVVFLPDCHESLAGIVAGRIRERYNKPVFVLTRAEGC
                     AKGSGRSIEAYHMFHALVEVKDLLLKFGGHPMAAGFSLEEADVGEFRRRLNENAADKL
                     TPDDFIPRVWIDVAMPFEYISEELICDLERLEPFGQGNEKPQFAQKAMGIRSARVMGK
                     NRNVVRLSLVNERGFSMDGIVFGDGDAFMEEMGSNRQIDIIYYPTVNEYNGNRSLQVV
                     IKAWKFKN"
BASE COUNT         3948 a         4345 c         3399 g         4139 t
ORIGIN      
        1 gaatcagaac ttttagatgc attctttgtc tttataaaac gttttaaaac cctgatccac
       61 tttaacggcg accggtttga cattccctat ctggaaaaat gctgccgcag gctggggcct
      121 tcccacagct ttgagggcat ggaaagtatg gatatctaca aaaaaatccg tccttaccgg
      181 aagcttctgg gattagaaag catgaagcag aaggccattg agcagttttt aggcatttcc
      241 cgtgaggata agtacactgg aggacagtta attgaggtct accgcgatta tctgcacacc
      301 catgaaaaat cgctctatga tctgctgatc cttcacaatg aggatgattt aaagggaatg
      361 cccgctatcc ttcccatttt aagctatgca gatttctttt catcccctct tttccctcag
      421 gctcctgaat tatgggaggg tgactgtaca gagcccacgg acacgcccct tctgcatctt
      481 atgggtaccg gctctgtttc tgttcccgtt ccgctggaat attcttccgg tccggtgagt
      541 ctggagctat atgaaaataa agccgtctgc accatccgcc tctgccgcgg ctgccttaaa
      601 tatttttatc ccgattataa ggattactac tatctcccct ttgaagacac agccgtccat
      661 aaaagcgtag gggagtatgt agatcggtct gcccggaaaa aggccactgc ccgaacctgc
      721 tacacaaaaa aagagggcct gttcctccct cagtttggcc cctttaagga accttccttc
      781 cgggaggatt acaagtcacc tctcacctat gtgctttatg atgactcgct gttttcacag
      841 ccccaggccg cagataccta cttcagtcag ctcttttgct ggcttttgga acagtagggc
      901 tgaaaacatt acgggtgatg aaaaatcccc tctaacagag ctccggctgc ggtaaaaaga
      961 agcagaagaa aaaaagcggc cagatgaaga cggtgttccc gtcctccggc ccagcctgca
     1021 aggactgccc aggtaatccc ataaatcacc ccctgtggaa acaaaagcca gagaaacact
     1081 ggaagtcccc agacgccctt ttccaatgtg agaaaagcca aggccccgga aaggcacatt
     1141 ccgccccagc aggtaatgca gccaaaaaag aaggggctgc agatcgttag acctgccacc
     1201 catcctgcag tcatctgaag cagccgcttt gccagcagac tcacaacgcc ggcttccttc
     1261 attgcctgtt ccagcccccc atctttcaat gtctctgtca ggcaggaaat catccccttc
     1321 ccccagagat cctccctgcc ttctcctgca agcaagcccc ttcccagtgc tccttctcca
     1381 aataaaagca caacggctgt tccccctatg atcccggctc caaagcagaa tataagccag
     1441 tcctcatagg aaaaacgccg ctgtctgaaa gctgtcaccg ttctggctgc cagcgccatt
     1501 tttttccctc tgatcttcat tcccgtttct ccctgtttcc ccttaaaaca ggtgtatgcg
     1561 gccggaggga tccgtatgta tcagggagtc tcccagtaca ggattccgtt tcggttctct
     1621 cttaaggcaa agtcctcatc atagaggtat tccagacagg aaaatacgaa tttcatccac
     1681 accggctccc gtgctgtaca gtttcacttc ataaattccc ggcgcacgtt cttccatcct
     1741 gctcaccctt ctgttttata caaactcgtc cttcttatcc acactccggt acttacgtcc
     1801 gtaagccata atggcggcaa tggtctttcc gtctgtgatt tttcccgtaa agatcagctc
     1861 ctctaaatcc ttaagctccc aggcctctac ttctatgacc tcatcctcat ccagatgctg
     1921 atggcttggc accagattgt gggcaacaaa aatatcaatg gcctcatcgc agaaggctac
     1981 cgtagtatta acactgatca gatattccag tttttctgtc ttaaagcccg tttcctcctc
     2041 cagttcccgg tacgcacact caattttggg ttctttggga tcatccagct ttcctgccgg
     2101 gatctccaga gtaaaacgat ccagtgcgtt tctgtactgg cgcaccatga ggatttttcc
     2161 ttcatccgtt accggcagca ctgccgcagc tccgtcatgg tggatatagt cccaatgggc
     2221 ctcatggccg ttggcaagaa ccgtatcctc gtaaatcttt aaaatatttc cctgatattt
     2281 tagctgccga tccagacgga tcaccggcat tccttcattt tctggtttct gtgccatgtt
     2341 ttttcctccc ctatttcatt ttctggcttt tttcatagca ggcgtctgcc gctttgatca
     2401 ctgcattgcg aaatccaaat tcttccagag cagatactcc cgcaatggtg gttccgcccg
     2461 gagagcagac ctcatccttt aactgccccg gatgctttcc cgtttccagt accattttag
     2521 cgcttccaag aacggtctgg gctgccattt cacaggctgt tttacgggga agcccatatt
     2581 tcacgccgct gtctgccagt gcctcgataa acatgtatac aaaggccgga gagcagccgc
     2641 tgacgcagcc cacagcctcc atctggctct cctctatcag ctccacgccg ccacagcttt
     2701 taaagaccgc gcagatctgc tccttttcct cctctgtaaa tggatcagtg ccaaaggcga
     2761 tccccgtcat tcccgctccc agcatagccg gagtatttgg catggcccgc accacacgga
     2821 tctcatctcc cagccgctcc tttaaactgg aaatggtctg tcccggagcc agggagatca
     2881 ccacctgatc ctcctctaat acctcgcgga tctcatcaaa tactacatca aaatactggg
     2941 gcttaaccgc aaggatcaga tatttgcagc tctgcgcgca gacccggttg gactctgcat
     3001 gaggcacgcc ggtagcttct gtaaccgcag ccatcttttc tgcatgagct gccgtaaaaa
     3061 taaatcttcc cggctcacag gtctttaaaa gaccgtgcag gatcgccagg cccatatttc
     3121 ccattccgat aaaaccgatc tcagccattt taagacctcc ttattctgca tcctttagta
     3181 tgttacaaaa atcaaaggta gcgaagtaat cctttggcgt ctctgccctc cggatcattt
     3241 ctgccgagcc gtcttctttt aaaagaagct ctgctgatct taattttcca ttgtaattat
     3301 atcccatggc aaaaccgtgg gctccagtat catggatcac cagcagatca cccttttcaa
     3361 tctcaggcag catccggtca atggcaaatt tatcattatt ttcgcacagg gaacccacta
     3421 tatcatacat atggtcacag ggtgcatttt cctttcccat caccgtaata tgatggtagg
     3481 cgccgtacat ggcgggacgc atcaggttta ccgcacaggc gtctacaccc acatactctt
     3541 tatgggtatg cttctcatgg atggcctttg taaccagaca gccatagggg cccagcataa
     3601 aacggcccag ctctgtgcac agcgctacat cacccatacc cgcaggcacc aaaacctcct
     3661 catagacctg ccgcacccct tcgccgatgg cgcggatatc attaggctcc tgatccggac
     3721 ggtaagggat gccgatacct ccggaaagat tgataaagga aatatgagcc cccgtttcct
     3781 catggaggcg gactgccagc tcaaacagga tctttgccag catggggtaa tactcatttg
     3841 tcaccgtgtt gcttgccaaa aaggcatgaa tgccgaaatt ctttgctccc ttggctttta
     3901 agatcttaaa tgcctcaaat atctgttccg ttgtcatacc gtacttggaa tcgccgggat
     3961 tatccataat atcattgctg atcttaaaaa caccgccggg attaaagcgg cagctgatgg
     4021 tctcaggaat ataccccagc gtctgctcca cacactcaat atgggtaata tcatccagat
     4081 tgatgatcgc ccccagcttt gcagcatagg caaattcctc cagaggcgta tcattggaag
     4141 aaaacataat gtcgtttccg gaaaaaccgc aggcctttga catcataagc tctgtcatgg
     4201 aggagcagtc cgtaccgcag ccatactcct ttaaaatatt taaaataaag ggattaggcg
     4261 tagcctttac cgcaaagtat tccttatatc ccttattcca tgaaaatgcc tcctttaact
     4321 gtctggcatt ttccctgatc cctttttcat catacaggtg aaacggcgtt ggatacgtct
     4381 ttgtaatctc ctccaactgc tcttttgtaa caaatggttt cttctccaca gcaaatttcc
     4441 tccttcgtcc gctttgctcc aaaactgtaa aaagtctgtc tgaaagcaca atgtccttgc
     4501 caaacattgt actcccgggc agacttcttt gtccagttca atttttagtt aatttctacc
     4561 acacgcatga tgttggtctg ggcagcctcc tcatggcgtc tcgcatagct taccacaccg
     4621 gcagtcagta cagccagctt tccgctttcc acgaaattct tttccttaag ctcctcaatg
     4681 gaattttcga tcagctcatc ggtagaatct gcgcggcggg accatacggg agttacgccc
     4741 cactgaagct gcatctggcg taccgtagac atacttgggg acattcccag gatacgggca
     4801 gccggcttcc acttggagag catcatagag gtaaagcctg taatacttgg aacaatgata
     4861 taatcagcgc cgatatcatg ggcagtggaa acagaagcgc agcatacagc gttagaaacg
     4921 ttcttcatgt tctctgcgct taccttacgc tggcggtatg cagtgtaatc cagatagctt
     4981 tctgtctcct caacaatgga actcatcatc tttaacgcct caaccggata cttacccatg
     5041 gcagtctcgc cggaaagcat cacggcgtct gtaccgtcat acactgcgtt tgcaacgtct
     5101 gttacctctg cacgggtcgg tcttgggtta cggatcatgg aatccagcat ctgggttgca
     5161 gtaataacag tcttgcaggc cacattacac ttgcggatga tcatcttctg aatatttgga
     5221 accttctgag ccgggatctc aacacccatg tcgccgcggg caaccatgat gccgtcagcc
     5281 tcagcgataa tctcatccag attctcaatt ccctctgcat tctcaatctt tgcaattacc
     5341 tttatggaag agcctgcctc atccagcata gccttgatct cccggatgca gtctgccgtg
     5401 cggacaaagg aagctgcaat aaaatcaaag ccctgcttga tgccgaaacg gatatcttcc
     5461 ttatccttct cagtcagagc cggaagcttg atgcttacgt taggaacatt tacgcccttt
     5521 ctctcgccaa gctcgccgcc gtttacaacg gtacatacga tatctgtatc atttacctca
     5581 tttaccttta actcgatcag gccgtcatcg atcaaaatgg tgtttcctgc agaaacgtcc
     5641 tcatttaagc cgctgtaatt gatatggccg atggtatcat cgccaacgat ttctctggta
     5701 gtcagcgtat agatctgtcc ctcttttaaa gtgaccttct ttccgtcctt taaaatgccg
     5761 gtacggatct ccggtccctt tgtatccaga agggctgcaa ccggaatatc cagttccttg
     5821 cgcacttcct ttaagatttc cagacgtccc agatgctctg cataatcacc atgagaaaag
     5881 ttaaagcggg caacgtccat gccattcttt gccagcccca gcataacatt cttgtcattg
     5941 gtattgggac ccatggtaca gatgatcttt gtctttttca ttttaatcct ccattattaa
     6001 acgtaagttg tgtaaagcga tccggcgcag gctttaatat gtggggaagc tgcttccggg
     6061 aatttatgga aagaagcaaa acccggacta ttcctcaggt ttttctcctt ttttgacatg
     6121 tttgtgcgtg tataaatgat ccatgcagta ctcataactg ccttcgcatt tggaacagta
     6181 gcggaattcc atcccagggc tgtcctctga ggtgcgtccg cagactgcac agcggtgggt
     6241 atttttcacc acccggatct tctccgtctt tttctggaac tctctggaac gctttttctg
     6301 ataaaccggg cctttttttc caaaggtaag ccatccaaaa aagataccca taagagcgat
     6361 gcttaacaga atctctatcc ggtcgatcag gctgcctctc acaaaagaat agatatacat
     6421 taccccttca aatacggcaa tccagctgga ctttaaggga ataagcatca taaaaagcac
     6481 ctgcatatcc ggaaacatca gtgcgaacac aaagaagaat gagtacacca gataatttgg
     6541 gaacagcggc agaggcatac ctgtgatcag gtagatcagc acggaaacag ccgcatgaag
     6601 cagtacgccg gatataatat aacagttaaa cttaaaccgt ccccaaacct gctctaccag
     6661 caatccaaaa agccggacac agtaaatgcc aatggcatta aaaataacaa agctgatcgc
     6721 atctccaccc gtaactgccg gcggatatgc aagaaacgtt acaattctcc agatctgccc
     6781 ccggacaacc agcgcaggat tcaggatcag atagccaaaa aactccggag aaagcagata
     6841 ggtcacaacg cccaggatct gcaggccgca tacgatgtca atgatactgc ctcccatcat
     6901 acgcctcaga ctgcgctttg cattatttac aaaatgattc atttaaaatc ccctcatttc
     6961 aaaacagatc cttcttatta aaaatatacg ccacaattaa ggacagggcc actgcaaatc
     7021 ccaggacgat ccagaatcca taggggctgt ctgcaaaggg catccccttt ggcagcacat
     7081 tcatgccgta aaagctcgct accatagtcg ggatggacag gacaatggtc actgtggcca
     7141 aaaacttcat aacaatattc aggttgttgg aaataacgga agcaaaggta tccatagtac
     7201 cgcttaaaat tccattataa atactcgtca tctccatggc ctgcttgttc tcaatgataa
     7261 cgtcctccaa aagatctgta tcctccggat atttcttgat cttgtcaagc tttaacagac
     7321 gctccagcac cacttcattg gaacggagag acgtggtaaa gtataccagt gatttctgca
     7381 gctccaaaag ctcgatcagc tcttcatttt tctgagcctg atgaagtttg ttttcgatca
     7441 cttcgctctt cttgtcaatg atcctcaaat actgcagata ctgagtggca tttttataaa
     7501 gaatctgcag gataaatctg gttttcataa atgtatggaa atctctcacc ctgccgtcca
     7561 taaaagcgct taaaatgggc gtttcctcca ggcagaccgt gatcagcaca tccctggtag
     7621 taatgatcgc catagggatc gtaacaaacc agtctttccc gtttcgctcc tctatagatg
     7681 gtatatccac caggatcaga gtgtattcat cctctgcctc aatacgtgaa cgctcttctt
     7741 catccagagg cgcttttaaa tgatccggat caatgcggca ggcatccgct acatctatga
     7801 tttcagaagc ggtagggttc gtaagagcga tccagcttcc gggctgcatc tcttgctttt
     7861 catgaatcgt accatcttcg gttttaaaaa tccgtatcat gcgaactctc ccccattctg
     7921 aaattttacc ccctaattat agtgactgcc tacgattttg tcaaatggat tagaactcca
     7981 ttttacataa aacgcaattt ttacaaacat cctacaattt attaacgttt ttttaacgtt
     8041 cttccctgat tgtatttgcc ttggatttgt gctacacttt agcgtgaaca tttttgtctg
     8101 ttgcttttgt cacagttttt atacgaattt tattataaaa tatacgccaa accagtgcag
     8161 cccatgttat ttggggctgc ctctttcata cagaagagtc ctggatcaga ctacaaagga
     8221 gggcagggtc aaaacccgta tctatgaatc agatgagtac aacttaccgt ttaggtgtcg
     8281 atatcggctc caccaccgta aaggtggctg tgctggatca ggaaaaccgt atcctttttt
     8341 ccgactatga acgccattat gccaatattc aggaaactct ggcaaagctt ttaaaaaaag
     8401 cggtatcaca gaccggagat attcttgtta ttcctgtgat caccggctcc ggcggcctga
     8461 cactctcctc ccatcttcat atcccctttg tacaggaggt cgtagccgtt gcctcctctc
     8521 ttcaggatta tgcgccccag actgacgtag ccattgagct gggcggtgag gacgcaaaga
     8581 tcatttattt taccggcgga atcgatcagc gcatgaatgg catctgtgcg ggaggcaccg
     8641 gctccttcat cgaccagatg gcctccctgc ttcagacgga cgcctccggc ttaaatgaat
     8701 acgccaaaaa ttatcaggcg atctacccca ttgcagcccg ctgcggtgta tttgccaaat
     8761 cggatatcca gcccctgatc aatgacggcg ctacccatga ggatctggcg gcttctattt
     8821 tccaggcagt ggtaaaccag accatcagcg gcctggcctg tggcaagcct atccggggaa
     8881 acgtggcatt tttaggcggt cccctccact tccttccgga gctgagaaag gcatttgtcc
     8941 gtacccttca cctgacgccg gagcagacca ttgccccgga gcactcccac ctgtttgctg
     9001 cggtgggcgc cgctatgaac cctaaagaag atcagacgcc cctttccata gcggatctga
     9061 tcaaaaccct ttcctccggg gtgcagatgg attttgaggt aaagcgcatg gaacctcttt
     9121 ttaaaaacca ggaagagtat gatgcttttc ttgcagacca tgctcacaat catgtaagat
     9181 cctcggatct ttcttcctat gaagggcttt gttatctggg tattgatgcc ggctctacca
     9241 ctacaaaagt ggcccttgta ggcgaggacg gctccctttt ataccgtttt tatgaaaata
     9301 acaacggaag ccctcttgca gcagccatcc aggcggccag agagataaac gagcagatga
     9361 cagacaaggc aaaaatcgca tggtcctgct ccacgggata tggagaagcc ctgttaaaag
     9421 ccgctttttt gctggacgag ggagaagtgg aaaccatttc ccattactat gcggccgcct
     9481 tctttgatcc tgaggtagac tgcatcctgg atatcggagg ccaggatatg aagtgtatcc
     9541 gcatcaagga cggcaccgta gacagcgtac agctaaacga ggcctgctcc tccggctgcg
     9601 gttccttcat cgaaaccttt gccaaaagct taaattacag tgtagcggat tttgcaaagg
     9661 aagcactttt tgccaaaaat cccatcgatc ttggcacacg ctgcaccgta tttatgaatt
     9721 ccaatgtaaa gcaggcccag aaggagggag cgaccgtagc agatatctct gccggactgg
     9781 cctactctgt gattaaaaac gctttattta aggtaatcaa aatcacaaat gcctctgatc
     9841 ttggaagcca tgtagtggtg cagggcggaa ccttctacaa tgacgccgtg ctccgcagct
     9901 ttgagcggat ctccggctgc cgggctgtcc gtcctgatat cgcaggcatc atgggcgcct
     9961 tcggagcagc cctgatcgcc agagagcgct accacctggg cggtgaaaaa gaaacctcta
    10021 ctatggcttt agacaagatc atttccttaa aatataccac ctccatggcc cggtgcaagg
    10081 gatgcaataa ccactgtgtc ctgactgtga accagtttgg aagcagccgc cggtatattt
    10141 ccggaaaccg ctgtgaacgt ggccttggaa tggaaaaagc caaaaaggaa attccgaacc
    10201 tttttgacta taaataccgc cggatgtttg actatgagcc ccttcccatg gatgaggcag
    10261 accggggctg cgtagggatc cccagagttt taaatatgta tgaaaatttc cccttctggg
    10321 ctgtattttt ccgtaagctg cgctaccatg tgatcctctc tccccagtcc acaaagcaga
    10381 tttatgagct gggtattgaa tccattccca gcgaatcgga gtgttatcct gccaagctgg
    10441 tccacggcca tgtatcatgg ctcatccgcc agggtgtgaa gtttatcttc tatccctgca
    10501 tcccctatga acgcaacgaa agccctgagg ccggaaacca ttataactgc cccatggtca
    10561 cctcctatgc ggaaaacatt aaaaataacg tagaagccct ggaagaggaa caggttcgct
    10621 ttatgaaccc ctttatggca tttacaaacg aaaaaatcct tacagatgcg ctggtaaagg
    10681 agttttccag ttctttccag atccctgcgg ctgagatccg tatggcagcc catgctgcct
    10741 ggcaggaatt aaccgcctcc agagaggata tcaagagaaa gggcgaagaa accctgaaat
    10801 ggatgaagga acacggcagg cgcggcattg ttctggcagg acgtccctac catgtggatc
    10861 cggaaatcca ccacggaatc ccggatgtga tcacctccta cggctttgcg gtgctcacgg
    10921 aagattctgt ttcccatctg ggaaaggtag aacggccttt agtggtaaca gaccagtgga
    10981 tgtaccattc ccgcctctat gcggcagcca gctttgtaaa aacccaggac aatctggatc
    11041 tgatccagct caattccttt ggctgcggtc tggacgccgt taccacagac caggtttccg
    11101 acatcctcac acggtccgga aagctttaca ctgtgcttaa aatcgacgag gtgaataacc
    11161 ttggctctgc ccgcatccgg atccgctccc tcattgcggc catccgtgta agagaccagc
    11221 gccattatga gcgcaagatc atttccagcg cctataaccg cgtggtattt acaaaggaaa
    11281 tgaagaagga ctacaccctt ctctgccccc agatgtcgcc catccacttt gatctggtag
    11341 agcccgcgat ccgggccttt ggctacaata tcgaaatcct tcagaaccac aataaaaatg
    11401 cagtagatgt ggggcttcgc tatgtgaata acgacgcctg ctatccctct ctcatcgtca
    11461 ttgggcagat catggatgca cttctgtctg gaaaatacga tttaaaccgt acagccatct
    11521 ttatgaccca gaccggaggc ggatgccgcg cctccaacta cataggcttt atccgccgtg
    11581 cccttgaaaa agccggcatg agccagattc ctgtggtttc catcaatgca aacggcatgg
    11641 aaaccaatcc gggcttttcc atcacccttc ctctgcttac aaaggctatg caggcggtgg
    11701 tatacggcga tatctttatg cgggttctct acgcgacccg accttatgaa gcagttccgg
    11761 gaagcgcaga tgcgctccat gaaaaatgga agaaacgctg tatcgcctcc ctgtccaagc
    11821 gctctgcagc tatgatggag tttggccgga acatcaaggg aatcatccgc gattttgacc
    11881 gtctggagcg aagggatatc aaaaagccaa aggtaggaat cgtaggagaa atccttgtca
    11941 aattctctcc cctggcaaat aaccatatcg tagagcttct ggaagccgaa ggagcggaag
    12001 ccgtgatgcc ggatctgatg gacttcctgc tttactgctt ctacaacaac aatttcaagg
    12061 cagagcatct gggcaagaag aagtccactg cccggctgtg caatgcggga attgcccttt
    12121 tggaatactt ccgccggacg gccagaaaag agctggctgc aagcagacat tttgcacctc
    12181 ccgcagccat caatgagctg gctgatatgg caaagggctt tgtatccctg ggcaatcaga
    12241 ccggagaagg atggttcctt acaggagaaa tgctggagct aatccacagc ggcgtgaaaa
    12301 acatcgtctg cacccagccc tttggctgcc tgccaaacca tatcgtagga aagggcgtga
    12361 tcaaagagct tcgcaggaat tatcccgact ccaatattat tgccgtagac tacgatcccg
    12421 gtgccagcga ggtcaaccag ttaaaccgca tcaagcttat gctggcaacg gcgcataaaa
    12481 atctgaaaaa agagatggaa ccgaaggttc aggtgtaaaa ttaaagagga tctgccgcac
    12541 ccgatggata tggcaggatc ctctttttct tttgtatgtt aatcttttat tttacccttc
    12601 atacagcgga tacttatcgc aaagttttgc aacctccgcc ttaatatact ctttcttatt
    12661 ttcaaaatcc gttgcagtca gccagataca ttctgcgatc ttttccatgt cctcttcctt
    12721 taaacctctg gtggtgacag ccggtgtacc gatgcggacg ccggaggtaa caaaggggct
    12781 tctggggtca ttgggaacgg tatttttatt caatgtaatg catacctcat cacagcggtt
    12841 ctgaagctct tttccggaaa tatccagacc gcgcaggtca accagcatca gatgattgtc
    12901 cgtaccgtcg gttaaaagtt tgaatccctg ctttttaaga gcctctgcaa gggcctttgc
    12961 atttaaaaca acctgatggg catattcctt aaattcaggc tttaaggctt cgcccagaca
    13021 cactgccttg gctgcgatca catgctctaa gggaccgccc tgggttcccg ggaaaatggc
    13081 cttattgaag ttaaacttct ctgccgcctc cttatttgcc aggatcatgc cgcctctggg
    13141 acctctgagc gtcttatggg tggtagtagt cactacgtct gcgtatggaa tggggcttgg
    13201 atgctctccc gttgccacta aacctgcaat atgggccata tccaccatca gataggcgcc
    13261 tacctcgtcc gcaatctcac ggaagcgctt aaagtcaatg gttcttgcat aggcgctggc
    13321 tcccgccacg atcagctttg gcttcgattc cctggccttg cgctctacct cgtcatagtc
    13381 aatgaatccc tgatcgtcta ctccgtaggg tacaatatta aaataaaggc cggaaaagtt
    13441 tacaggactt ccgtgggtca gatggccgcc gtgatccaaa ttcataccca taaccgtatc
    13501 tcccggcttt aacatggcta caaataccgc catattggcc tgtgcgccgg aatggggctg
    13561 tacgttggcg taatcacagc caaacagctt ctttgcacgt tcgattgcca gatcctccac
    13621 tacatccaca cactggcagc cgccgtaata gcgctttcct gagtatcctt ccgcatattt
    13681 atttgtaaga actgttccca ttgccatcat taccggctcc gaaacaatat tttcagaagc
    13741 gatcagctcc agattctgtc tctggcgtct gcactccgcc tgaatcgcct cgcccacttc
    13801 cttgtcatag cctgtaataa aatccataac tgtatttacc attgcagcct accgtccttt
    13861 ccgctctttt ctgcgattat aacctatctg attataaaat gcaatactcc ctgatacgga
    13921 cacattttcg gttttgtctg caaaataact gtcaattctt aaatttccat gcttttatca
    13981 ctacctgaag gctccggttt ccattatatt catttacagt gggataataa ataatgtcaa
    14041 tctgacggtt tgaacccatt tcctccataa aggcatctcc atccccgaac acaatcccat
    14101 ccatggaaaa gccccgttca tttacaagag aaagacgcac tacattgcgg ttttttccca
    14161 ttacacgggc gcttcgtatc cccatggcct tctgagcaaa ctggggcttt tcattgccct
    14221 gtccaaaagg ctcaagccgt tccagatcgc agatcagctc ctctgaaata tattcaaagg
    14281 gcattgccac atcaatccat actctgggga taaaatcatc aggcgtaagc ttatctgccg
    14341 cattttcatt gagccgtctg cggaattctc ctacatctgc ttcttccagt gaaaaaccag
    14401 ccgccatggg atggcctccg aatttcagca gcagatcctt tacctctacc agggcatgaa
    14461 acatatggta ggcctcgata gaacggccgg atcccttggc gcatccttct gcccttgtaa
    14521 ggacaaaaac aggcttattg taccgctctc tgatccgtcc ggctacaatt ccagccaggg
    14581 actcatggca gtcgggcaga aagaccacta aaaccttgtc atccttaagc tctgtttcca
    14641 cctgaagcgc cgccgcatcc acattctcct gcgtcatagc ctttctccgg tcgtttaatt
    14701 cctttaattc ctctgcaata gaatccgccc ttgcagtatc ttcgcacagc aaaagctcca
    14761 tggccagtct ggccgtctga agccgtccgc tggcatttaa acagggcccc aggacaaaac
    14821 caatatggta cgcactgata tttgccggat ccagcgcatt tttataaatc aggcttttaa
    14881 ggcccggatt tccggtcaca gccagacgct ttaagccctc ctttaccaca atcctgtttt
    14941 catccttcag cttcatcaca tctcccaccg tagcaatggc agcaagctcc agaagttcaa
    15001 gccattcctt acggctgatg ccgtatgctt catagagagc gccaacaagc ttaaagacca
    15061 ccatggctcc gcagatctcc ggccacggat accggctgtc cctgcgcttg ggatttacaa
    15121 ttacatctgc gggaggcatc aggtcttccc ctgtttcctc attggtctgg atatcgtggt
    15181 gatctgtaat gatcacctcc agccccagct cctttgccag ggcaatctgg ctgatggcgg
    15241 caatgccgtt gtcacaggtt acaatggtct gtatcccatc tgccgccgca gcctcaatga
    15301 tatgctcgtt gatgccgtaa ccgtcccgga tccgctctgg gatcacatag tccgcacagg
    15361 ctccgatccg tttgaatccc ttatataaaa tagcggtgga acatacgccg tcgatatcat
    15421 aatcccccac aatccgcagg cgccgttttt cctgtactgc ccggcagatc cgctgaaccg
    15481 cctcctccat atcgggcaaa agcccggggg aataaagatc ctccaggctt ccattcagat
    15541 agcggtcaaa ttccgcttcg ctttcaatat ccctgtttcg tataattctg gctgtcaccg
    15601 gactgatgtg aaaccgtcct gacagagcgt taaaatcagc tttttttgca tgaagcatcc
    15661 agattgatcc tgtcatcctg ctgtcctttc tcttatgtca tttctgggga tttttcttac
    15721 ccctgcccat cccccgtttc atttcctccg ggaatcatcc ttccaagcag cccgccaatc
    15781 acagccggca gcagccatcc aagaccggga gcatagcctg gaagatgcct c
//
LOCUS       DBMDUH010000048        15649 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_2100_length_15649_cov_5.885130, whole genome shotgun sequence.
ACCESSION   DBMDUH010000048 DBMDUH010000000
VERSION     DBMDUH010000048.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 15649)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 15649)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15649
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2100_length_15649_cov_5.885130"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="ACZPCG_05870"
     CDS             complement(<1..714)
                     /locus_tag="ACZPCG_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003526066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR1284634.1"
                     /translation="MKLNTGMRCHDLCEKMEMEKVFQLAKENGVHQIQLAFAKSISNY
                     DFTTGHYNPGFARYIKEQLDKNEIHVAVLGCYINPTNPVEELRKKEVARFIEHLKYAR
                     IIGADMVGTETGRLSSDFRVTPETYSEDGYQLLLKSMREIVSAAEKLGVIVGVEGVFD
                     HTLYSPARMKRFLDDIDSPNVEVILDSVNLIHPDEVAPEAQERVIRQAFEYYGDRISA
                     LHVKDFVFDGQNQLFRHVGE"
     gene            complement(729..1298)
                     /locus_tag="ACZPCG_05875"
     CDS             complement(729..1298)
                     /locus_tag="ACZPCG_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HIR1284635.1"
                     /translation="MTRLATLFTCSFRRLKEVRTVTVCAMLGAIAIVLGSLSIDLGPS
                     VRIGFSGIPNELVHYLFGPAAGSLFAGALDILKYMIKPVGGFFPGFTLVAMLAGVIYG
                     SFYYRKPLSLGRVFAAHLVVSLVCNVLLNTLCLSLLYGKAFMVLLPPRVIKNLIMWPI
                     DSLIFFHIAKLLEAAGIFRFLGGRDQNRL"
     regulatory      complement(1371..1487)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            complement(1620..1850)
                     /locus_tag="ACZPCG_05880"
     CDS             complement(1620..1850)
                     /locus_tag="ACZPCG_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HIR1284636.1"
                     /translation="MKRVAVKFNQPDQIIDFVRIMNRYEYDVDVKCGSRIVDAKSIVG
                     VLALAKSRTVEVIVHTDESCEDLMEEISRYAA"
     gene            2132..3679
                     /locus_tag="ACZPCG_05885"
     CDS             2132..3679
                     /locus_tag="ACZPCG_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1284637.1"
                     /translation="MKLLIADDEKLTREGICSLLDLKQYGITEVILADDGVNALTEAR
                     KNRPDILLTDVRMPRMTGVEMASLYLEEAPDTAVIFMSAYSDREYLKAAIKLKAVSYI
                     DKPAEKKELEDAIKEAADRFRTLRAAKAGAMLEEKDLRGHLALALTNSSKLEEALELA
                     ARVSPKVETDFWFTCVITSCLTPLSELPSEDLIQENQLFSSYLKKMGIVRILVPKADR
                     YIVSFLCSSNRQEPETVRAFTSFLKERITPYCRFFIASGPSVQGMDQAAFSYKSAMSL
                     LSESFYHDYNSILTQTPPAGSLQPAEDPFADFTAALAAQRELEALKAAGNFYNALKNS
                     PPLPEAQVRDRYFRYLNKLDEISLANHISLWKREGIESESIWEDVISCATLGELHDFL
                     CHRIRHYFQSLIFNQSEHPVVFQIKEYIRQNFAVPTLSVLDISQYANRSSSYICTLFK
                     NETGQTINQYLTDYRIKMSKQFLSDPRYRITDISAKVGYSDGNYYSKAFRKIVGLSPS
                     EYREKIL"
     gene            3679..5523
                     /locus_tag="ACZPCG_05890"
     CDS             3679..5523
                     /locus_tag="ACZPCG_05890"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1284638.1"
                     /translation="MKRPIKQFFHYYIYDMKLKKKLALSFAILFMLPTLTVTFFLFTR
                     IFRIVLNDTLRSEEAFHSQSALAAENLMIHVAHASDTLTYSLPIQRLFSITKKQAPGA
                     ALSRTHLNNLYQLADMAAEPSLIRTIRIYYDDSSYPQLDILNNGRQRLFIPLSAIDSG
                     WLDRFESSPEDRLLCAAPDLSENEKKYCGNLAYLVRLNYLSKDERAEKEAAAYVAVYF
                     SRSSLQQLITHTHLIQGEINFFMNASGEPIDMKDERLSSSIPQIPHSLFEQLKQKEQF
                     IPADLDGSLVYAASFSINGSDWYLMSVIPRQSLKTAGVSMILQFIAFYGVISLLALFL
                     AYRLSKSFADRIIHVALQMETIRSGRPRPLLLKNAGNDEIGILSGAYNFMTAEINELL
                     NQKELAAKELQKAEFRALQAQINPHFLYNTLDMINWLAKSGQISQVSQAVQALTRFYR
                     LTLGQKELFGTIDDEITHVTLYMQLQNMRYNHCADFIADIPPELGSLTIPKLTFQPIV
                     ENALLHGILMKEEKKGCILLTGWREGEDAVLVISDDGAGIAPEKLDTLLDSLSADNGL
                     TSLRHIGVSNTNLRLKSLYGDAYGLSFTSTPGQGTEVTVRIPARPVSL"
     gene            complement(5551..5676)
                     /locus_tag="ACZPCG_05895"
     CDS             complement(5551..5676)
                     /locus_tag="ACZPCG_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284639.1"
                     /translation="MKKIVKKCEAFLCMAAVIIGVTALRFQLFAPEVLEQLTMQL"
     gene            complement(5777..6652)
                     /locus_tag="ACZPCG_05900"
     CDS             complement(5777..6652)
                     /locus_tag="ACZPCG_05900"
                     /inference="COORDINATES: protein motif:HMM:NF024803.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR1284640.1"
                     /translation="MSRTIKEAEELTKVTSQNIRYYEKQGLLAPKRDKENSYRLYSEE
                     EISRLKLIRLFRKLGMPIGEIRRMFEGDISLEEAIGDQISRLQSQKEELTAALEFCGR
                     IKENQLADVDPDLYLEEMEQEEKRGSVFMDFVNDYARVVRSEAIREFSFMPDDRCDEP
                     DTFERELLKYGKENGKTIIITKKSRSPHFMMDGKEYRAYRTSSRFGIVVHCELVHPED
                     YVPEGMSRRKYRLLRVISIAVLPLILFAASNLWIFKQMDFGSPLTWLALFVLAVVLLA
                     DLCFLYYSCGKNFRG"
     gene            complement(6798..6881)
                     /locus_tag="ACZPCG_05905"
     tRNA            complement(6798..6881)
                     /locus_tag="ACZPCG_05905"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6845..6847),aa:Leu,seq:cag)
     gene            complement(6885..6968)
                     /locus_tag="ACZPCG_05910"
     tRNA            complement(6885..6968)
                     /locus_tag="ACZPCG_05910"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6932..6934),aa:Leu,seq:aag)
     gene            complement(7069..8202)
                     /locus_tag="ACZPCG_05915"
     CDS             complement(7069..8202)
                     /locus_tag="ACZPCG_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284641.1"
                     /translation="MKRAVIWFLLSCKRCLRKGIFPLLLLLMPLAAFVAGRLSDKEEG
                     EIPVAVSVSGEEDVSERSLAWRLAERLADRKDGMFDFYICSDEAQVRDEVASRRAECG
                     YVIDSGLEEKLDQKQWKRVIRLYMAPSTVAGSISTETVFSELAALYNRNLLMDYVEEG
                     EAFAGMGPVNSSSRAKAAEEAGERYDRWNQEEGLFHFQYIYETNGGGGAEDGVETQSP
                     KSDAAGSIFPVRGLGAVMVFILSLYGGVMAGEDERRGLFMPLGIGLRLPCRLAAAAAP
                     GALACLSVFCALFLGGMAEGGSFGAVGRELGLLLLYGCGCTVFAFGLKLIVRSPELLC
                     CMIPFFIIGSLLFCPVFVDPGRWIPGMEQLGRLFLPYYYLRCF"
     gene            complement(8199..9386)
                     /locus_tag="ACZPCG_05920"
     CDS             complement(8199..9386)
                     /locus_tag="ACZPCG_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1284642.1"
                     /translation="MRGYLCYAGMELKRALKRLPHILAGAAVLVVLMGAAALLAGKLL
                     YGGQTVERIPVGVVLPEEDALAGQAMRMVSSLESVKSLCDFTYIEEEATGEKLKKGEL
                     FAVLKMPDGFIQDIMSGVNTPVTVILPENAGAESRIFKELTDAGAEILGSAQAGIYSG
                     DELLSEYGLTDKMAVFERDMNQIYLTYSLPRMDYFRKVMVNAAKDVSVPVFYGISAFV
                     LVLLLSVISVSDLLKREPAVMRQKLSLMGIGPFTTAGSRILGVSVLLSVISVLAAAAA
                     AWTGMISIGLNTILTGFLVCLGAASFSVCLFQTAGSRIGGIFLQFFLVTAFHFLSGGF
                     LPEVFLPESIQRAAPFLPTHILMEGVKMAVTGNESLVTAVELAALGVGGWLIAAAAEV
                     KEA"
     gene            complement(9400..11037)
                     /locus_tag="ACZPCG_05925"
     CDS             complement(9400..11037)
                     /locus_tag="ACZPCG_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR1284643.1"
                     /translation="MEMTKKMKMAAVGAAAVAVVGGGVFAYTRLAGGDPKETVIQAFE
                     NVYTEGQTDPMEELFGLSEFAKARVSASQNSGLMLKLDSCSEPAVNTYAGSGLRIDAK
                     NDVENNKVSANMGIIYNGMDLVNLDLYYGDNTVMAAVPELSPKVFTLDFGEGLEERLK
                     NSPMLGSALEQSGVDASVMAEYMELLAEQARQTQEGQATSFDLKALMKRYREGCKAED
                     DFKAALTVEKGEKASFTIDGKEQACRGYEVTVSKEAMINFLRTSSDFFLQDEVLKKDF
                     LKRLELSVKLSQLAGAQMEGQDFPTAQEMQEQTYEEARTEIDGMIAFLDSSLNDVSMT
                     VYVDKEGCLASVKGTTSFNSTGSEAEPVQLQFGCELKGGAYPTQNMSAQAVLENGAAS
                     VQIEAVKEGAYDGKELTSGFELSIENQGEIAEKWDITLDSSYNSEGGGFDVQAAAAQD
                     GMELLGFSAQGVVDELEKGKNIHLTLDSLDVSAMGDTGNAVLSGEYYIRPLTEEVVPL
                     EGDTMDVLAAGEEEWNSVLMEVLFSFISLSGQMDTGN"
     gene            complement(11119..11790)
                     /locus_tag="ACZPCG_05930"
     CDS             complement(11119..11790)
                     /locus_tag="ACZPCG_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1284644.1"
                     /translation="MLRASGICKSYGKRRIIEQAEFFAGRGSCVGVVGANGCGKTTFL
                     SILAGTRKADRGSVKIDGEEALGHPSVFARNVAYVPQENPLMEELTVRDNLLLWHRGN
                     KKQMNEDLKTGSAALLGIPAMLGMRAGKLSGGMKKRLSIACALSGNAPVLIMDEPGAA
                     LDLECKESIRQYLKGYVNEGKTVILTSHEMEELSLCDRLYVMKDRCLKEIEAGLSSRE
                     LIHYF"
     gene            12059..12398
                     /gene="ssrA"
                     /locus_tag="ACZPCG_05935"
     tmRNA           12059..12398
                     /gene="ssrA"
                     /locus_tag="ACZPCG_05935"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(12693..12962)
                     /locus_tag="ACZPCG_05940"
     CDS             complement(12693..12962)
                     /locus_tag="ACZPCG_05940"
                     /inference="COORDINATES: protein motif:HMM:NF044580.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6809 family protein"
                     /protein_id="HIR1284645.1"
                     /translation="MNIIRQIYNGDYRPADQEPEETPEHQAARERAYREYQALRDKLP
                     MEQRPELDQLIDAHLEASLYGYEAAYTQGARAGAQILLDLLDGCR"
     gene            complement(13527..14402)
                     /locus_tag="ACZPCG_05945"
     CDS             complement(13527..14402)
                     /locus_tag="ACZPCG_05945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR1284646.1"
                     /translation="MTNEQLAARIRAGEDVGENMAQLYDQVKDFIRSVAWKYKGLGEL
                     EDLEQEGFLALYDAIDHYEADQGVKFLSYAEYWIRQRMQRYLQVNGSCLRLPVNRQEA
                     ISRYKRFCLSFQLEHGRGPSEAEIGRELWLSVDQVRRLQLDACMSHVCSLDAPVRGRE
                     GEEDITLGDLAPSACDLEEEATERIDHESLCSLLWECVDSLPGKQPDVIRSRYQGGLT
                     MGRVGEVCGITEAEARKQHMKALRALRNPENSNRLRRFVPEDERVYSSALWGNGAEHF
                     NRTWTSSTERVALGL"
     gene            complement(14420..14554)
                     /locus_tag="ACZPCG_05950"
     CDS             complement(14420..14554)
                     /locus_tag="ACZPCG_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284647.1"
                     /translation="MESGSVIRFRVCSEVYQMGIITEFITIVAGVLIGEWIARRIDRK
                     "
     gene            complement(14536..15273)
                     /locus_tag="ACZPCG_05955"
     CDS             complement(14536..15273)
                     /locus_tag="ACZPCG_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284648.1"
                     /translation="MEKITEKILNEFHEQDNKYVRVVREFGTVRNIPGNRELALRYIQ
                     QIKEETYNRIKNIVDEEINALETGSKGQSTDDNSTAALLKKSNMIALMQAEINGSEDH
                     ELIDLVDEYRSTEYFDLFKTMIGPRLREMRLGDGMQKRAANEIMRRFESAQLPEIKKL
                     KEQLNHIHAIQFFNSGTITLSPCPTEEGIFLLGNGQRRNIAFDLDAATADFNPLEETQ
                     EPLVISESVGKYTVKSIKHLFPWNQEA"
BASE COUNT         4075 a         4312 c         3041 g         4221 t
ORIGIN      
        1 ctcgccaacg tgcctaaaca gctgattctg gccatcaaac acaaagtcct tcacatgaag
       61 ggcgctgatc cggtcgccgt aatactcaaa agcctggcga atgactcttt cctgagcctc
      121 aggcgccacc tcatccggat ggatcaggtt cacactgtct aaaatcacct ctacgttcgg
      181 ggaatcaatg tcatccaaaa accgcttcat ccgcgccgga ctgtaaaggg tatggtcgaa
      241 tacgccctcc actcctacga tcactcccag cttctcagca gcagagacaa tctctctcat
      301 gctctttaac agaagctgat aaccgtcctc tgaatacgtt tcaggagtca cacgaaaatc
      361 cgaggacaga cggccggttt ctgttcctac catatctgct ccgatgatgc gggcatattt
      421 taaatgttca ataaaccggg ctacctcttt tttgcgcagc tcttctacag ggttagtagg
      481 gttaatatag cagccgagaa cagccacatg gatctcgttt ttatccagct gttctttaat
      541 gtagcgggca aatccagggt tgtaatgacc ggtggtaaag tcataattgc tgatggattt
      601 ggcaaaagca agctggatct ggtgcactcc attttccttt gccagctgga acaccttttc
      661 catctccatt ttttcacaca ggtcatggca gcgcataccg gtatttaatt tcatggcaac
      721 tcctcctttt ataaacggtt ctgatccctt ccgcccagga acctgaatat tcctgctgct
      781 tccaggagct ttgcaatatg aaagaagatc agggaatcaa tgggccacat gataagattt
      841 ttaatcactc ttggcggcaa aagcaccata aatgcctttc cgtaaagcag agacaggcac
      901 agcgtattaa gcagtacatt acaaaccagg ctcacaacaa ggtgagcggc aaatactcgc
      961 cccagactta agggctttct gtaatagaaa gaaccgtaga tcacacctgc cagcatcgcc
     1021 acaagggtaa aaccggggaa aaatccgccc acaggcttga tcatatattt taaaatatca
     1081 agcgcccccg caaacaggct tcctgccgca ggaccaaaca gatagtggac cagttcattt
     1141 ggaatacctg aaaagccaat acggacagac ggtcccagat caatgcttaa tgaccccagc
     1201 acgatagcga tagccccgag catggcacaa acggtaacgg tccttacctc ttttaaccgg
     1261 cggaaggaac aggtaaacaa agttgctaat cttgtcataa aaaaattacc tcctttgcag
     1321 atctctcaaa ctacaatcgg agataatcaa atccctatga tcttctctga tgcagcggga
     1381 tgcggcccat gcaccgcaga aggatcggca caaagccgct ccttactttc gtctgactgg
     1441 caactccctg tccagccggc acttaacgcg cataagccta ctctgcttat ttattttttc
     1501 atacataact atacacgttt ttggaagata tgcaagtttt ttttaagaag cataacacgc
     1561 actcaaacaa aatacgccca ttctgcttat atttaaacag aacgggcgca agcctcctat
     1621 tatgccgcat atctggaaat ttcttccatc aaatcttcac aggattcatc tgtgtgaaca
     1681 atcacctcca ctgtcctgga tttggccaga gccagcacac ctacgattga ctttgcatct
     1741 acgatcctgc ttccacattt tacatctaca tcatattcat accgattcat aattcgcaca
     1801 aaatcaatga tctgatccgg ctgattgaat ttaactgcaa cgcgcttcat attcattacc
     1861 tccatcatct gcgcaatact ctcatcacag agagcagaca tttataaaat tcattgccgg
     1921 atacacgggg atgacgctta cggtaagcaa tatccggcaa tgcatgataa catcttatat
     1981 ttcatatgat aacagctgaa gttgaaaaaa accatggtaa aatcctttca ttcgctgtaa
     2041 ttttttttga tttccattgc ataagtgaag gctttcaaat aaaataaagg cagagcgatt
     2101 gttgaatcaa aaaatggaga acctatatcc tatgaaatta ctgattgctg atgatgaaaa
     2161 acttacccgt gaaggtatat gcagcctgct ggatctgaaa cagtacggca ttacagaggt
     2221 aatcctggca gacgacgggg tcaacgccct tacagaggct agaaagaacc gcccagatat
     2281 cctgcttacc gatgtgcgga tgccccgcat gactggggtt gagatggctt ctctgtacct
     2341 ggaagaggct ccggatacag ccgtcatctt catgagcgcc tactcagacc gcgaatatct
     2401 gaaagcggca attaaactga aagctgtaag ctacattgac aaaccggcag aaaaaaagga
     2461 acttgaggat gccataaagg aagcagccga ccgtttccgt accctgagag ccgcaaaagc
     2521 cggcgctatg ctggaggaaa aggatctgcg cggtcacctt gccttggccc tgaccaattc
     2581 ttccaaactg gaggaagccc tggaactggc agcccgcgtt tctccgaaag tcgaaacaga
     2641 tttctggttt acctgtgtga tcacaagctg tctcaccccc ctttccgagc ttccctcaga
     2701 agatctgatc caggaaaatc agcttttttc ttcctatttg aaaaaaatgg ggatcgtgcg
     2761 gattctggtg cccaaggcag accgctatat cgtaagcttt ctgtgcagtt ccaaccgcca
     2821 ggaaccagaa acagtccgcg cttttaccag ctttctcaaa gagcggatca caccctactg
     2881 ccgttttttt attgcatcag gcccctctgt acagggcatg gatcaggctg cattttcgta
     2941 taaaagcgcc atgagcctat taagcgaaag cttttatcat gattacaatt ccatcttaac
     3001 acaaacacct cctgccggct ctctgcagcc tgccgaagat ccctttgcag actttacagc
     3061 agcccttgca gcccaaagag agctggaagc actaaaagcc gccggaaact tttataatgc
     3121 tctaaaaaac agccctcctc ttccggaagc tcaggtaaga gaccgatatt tccgatatct
     3181 gaacaagctg gatgagatca gtctggctaa tcacatttct ctgtggaaaa gagaaggtat
     3241 cgaatcagaa tccatctggg aggatgtaat aagctgcgcc accttaggag aactccatga
     3301 ttttctctgc cacaggatca ggcactattt tcaatccctg atctttaatc agtctgagca
     3361 tccggtcgtc tttcagatca aggaatatat acgccagaat tttgccgttc ctaccctgtc
     3421 tgtgctggat atcagccagt atgcaaaccg ctcctccagc tatatctgca ctctttttaa
     3481 aaatgaaacc ggccagacga tcaaccagta tctgaccgat taccggatta aaatgtccaa
     3541 gcagtttttg agcgatccca gataccggat cacggacatc tccgcaaaag tcggctacag
     3601 cgacggcaat tactacagca aggctttccg caagatcgta ggcctttcac catcggaata
     3661 cagggagaaa atactctgat gaaacgtccc ataaaacagt tttttcatta ttacatttat
     3721 gatatgaagc tgaaaaagaa actggcattg tcctttgcca tcctctttat gcttcccacg
     3781 ctgactgtga ccttcttttt atttacccgg attttccgca ttgtgttaaa tgacacgctg
     3841 cgctccgaag aggcctttca ttcccagtcc gctcttgcgg cagaaaatct tatgatccat
     3901 gtagctcatg cctctgatac actcacctat tcccttccga tccagcggct cttttccatc
     3961 acaaaaaaac aggcgcccgg agccgccctg tcacgcacgc atctgaacaa tctgtatcag
     4021 ctggcagata tggctgcgga accctcttta atccgcacca tacggatcta ttacgatgac
     4081 agctcctatc cacagcttga cattttaaat aacggccgcc agcgcctttt tatccctctc
     4141 tctgcaatag acagcggatg gctcgaccgt tttgagtcct ctccggagga cagactgttg
     4201 tgtgcagctc cggatctgtc ggaaaatgaa aaaaaatact gcgggaatct ggcctatctg
     4261 gttcggctta attatctttc caaagatgaa agggcagaaa aagaagcggc tgcctatgtg
     4321 gctgtttatt tttcccgcag ttccctgcag cagctgatca cacacacgca tttgatccag
     4381 ggagaaatca atttttttat gaacgcctcc ggtgaaccga ttgatatgaa agacgagcgg
     4441 ctctccagtt ccattcccca aatcccccac tcattatttg aacagttaaa acaaaaagaa
     4501 caattcattc cggctgatct ggacggttct cttgtatatg cggcatcatt ttccatcaat
     4561 ggatccgact ggtatcttat gtctgtcatc cccaggcaaa gcctaaagac agcaggcgtc
     4621 tctatgatcc tccaatttat tgcattctat ggcgttatct ccctgttggc cctgtttctg
     4681 gcttaccgcc tttcaaagtc ctttgcagac cgcattattc atgttgccct gcagatggaa
     4741 accatccgct ctggacgtcc cagacctctt cttctgaaaa atgccgggaa tgacgaaatc
     4801 ggcattcttt ccggtgccta caattttatg actgcagaga tcaatgaact gctaaaccag
     4861 aaggagcttg ctgccaaaga actgcaaaag gctgaatttc gcgccttgca ggcccagatc
     4921 aatcctcatt ttctctataa tactctggat atgatcaact ggctggcaaa atctggccag
     4981 atcagccagg taagccaggc agttcaggct ctcacccgct tttaccgtct gacgcttgga
     5041 caaaaagagc ttttcggtac cattgatgat gaaattaccc atgtaacgct ttatatgcag
     5101 cttcaaaata tgcgttacaa tcactgtgcc gactttatag cagacattcc tccagagctt
     5161 ggcagcctga cgatccccaa actgaccttc cagccaattg tggaaaacgc ccttctgcac
     5221 ggcatcttaa tgaaggaaga aaaaaagggc tgtattcttc ttaccggctg gcgcgaggga
     5281 gaagatgcag tccttgtgat cagcgatgac ggggccggga ttgcgccgga aaagctggat
     5341 acgcttttag acagcctctc agcagataat ggcttaactt ctctcaggca catcggcgtg
     5401 tccaacacca atctgcgttt aaaaagcctt tacggagatg cctacggtct ttcctttacc
     5461 agtactcccg gtcagggtac tgaggttact gtacgcattc ctgcccggcc ggtatccctt
     5521 tagcagcgtc ctcagcatca tcattcacac tcaaagctgc attgtgagct gttccaatac
     5581 ctccggtgcg aaaagctgaa accgaagcgc tgttactcca attatcactg ctgccataca
     5641 caaaaatgcc tcacactttt ttactatctt tttcattgtc aattcctcct catgttttaa
     5701 cgttgtccgt atcatagcac caaaggaatt aagatagcac tcaggtgtga ggcattcgta
     5761 ctaaaattgt attaaattat cctctgaaat tttttccgca gctgtagtac aaaaagcaca
     5821 gatctgccaa taacaccact gccagaacaa acagtgccag ccaggtgagc ggagagccaa
     5881 aatccatctg tttgaaaatc cacagattag aagcagcaaa cagaatgagc ggaaggactg
     5941 caatagaaat cacgcgaagc aggcggtatt tccttctact cattccttcg ggcacatagt
     6001 cctccggatg aaccagttca cagtgcacca cgatcccaaa tcttgaagaa gttctgtacg
     6061 ctctgtactc ctttccatcc atcataaaat gcggcgacct gcttttcttt gttataatga
     6121 tcgtttttcc attctctttt ccgtacttta acagctctct ctcaaaggta tctggctcat
     6181 cacaccggtc atcaggcata aaggaaaact cccggattgc ttctgatctt acaactctag
     6241 cataatcatt gacgaaatcc ataaataccg atccccgctt ttcctcctgt tccatttctt
     6301 ccagataaag atcggggtct acatctgcaa gctgattttc ctttatcctt ccgcaaaatt
     6361 ccagggctgc cgtcaattcc tccttctggg actgcaggcg gctgatctga tccccaatgg
     6421 cttcttccag agaaatgtcc ccttcaaaca tccggcggat ttctcctatg ggcatcccca
     6481 gcttgcgaaa caaacgtata agctttaagc ggctgatctc ctcctctgaa tacaaacgat
     6541 atgaattttc tttatctctt ttgggagcca gcagcccctg cttttcataa taccggatat
     6601 tctgactggt cacctttgtc agttcctctg cttctttgat ggttcgtgac atatacactc
     6661 ctccttaccc ctatggctta gggaaatatt ctttatttac acagtaagca taaggcttat
     6721 acctggtata atgtcaagat ttttccaaca aaaaaatccg aaactcaagt ttccttaagt
     6781 ttcggatcat catccagtgc agaagaaggg agtcgaaccc tcaaggtatt actaccacac
     6841 ggacctgaac cgtgcgcgtc tgccaattcc gccacttctg catatgcggg agatgggact
     6901 tgaacccaca cgagcataca cccacaagat ccttagtctt gcctgtctgc caattccagc
     6961 actcccgcag cacgtgttat aatatagcac agacttttcg atttggcaat accttttttt
     7021 caattttttc aatttttttc aaaaataatt tcaaagggta ttttctccct aaaaacacct
     7081 gagataatag tacggcaaaa agagtcttcc aagctgctcc atccccggaa tccatctccc
     7141 tggatctaca aacaccggac aaaataaaag acttcctatg ataaaaaacg gaatcataca
     7201 gcagagaagc tccggtgaac gcacaatcag ctttagccca aaggcaaata ccgtacagcc
     7261 gcatccatat aaaagcaaaa gccccagctc tcttcccaca gccccaaagc ttcctccctc
     7321 tgccatgccg cccagaaaaa gcgcacaaaa aacagataaa caggccaagg ctccgggcgc
     7381 tgctgcagct gccagacgac agggcagacg cagtccgatc cccaaaggca taaacaatcc
     7441 ccttctctcg tcttctcctg ccatcacgcc gccgtaaaga gacagaataa aaaccatgac
     7501 ggcccccaga ccgcgaaccg gaaaaatact gcctgctgca tcagattttg gactctgtgt
     7561 ctccaccccg tcctctgctc ctccccctcc gttcgtttca tatatatatt ggaaatgaaa
     7621 caggccctct tcctgattcc aacggtcata cctttctccc gcttcttctg ctgctttcgc
     7681 tctggagctg ctgttaacag gccccatccc tgcaaaagcc tccccttctt ccacataatc
     7741 cataagcaga tttctgttat aaagagctgc cagctctgaa aataccgttt ctgtagaaat
     7801 agagccggct accgtagaag gagccatata caaacgaatt acccgtttcc actgcttttg
     7861 atccagcttc tcttccagcc ctgaatcaat cacatatccg cactcagctc ttctggacgc
     7921 cacctcatcc cgtacctggg cttcatccga acagatataa aaatcaaaca tcccatcctt
     7981 cctgtctgcc aggcgttctg caagtctcca ggccagcgat cgttcagata catcctcctc
     8041 tcctgaaacg ctgacagcaa ctggaatctc cccctcttct ttatctgaaa gccttccggc
     8101 cacaaaggcc gccaacggca taagaagcaa aagaagagga aaaatcccct ttctcaggca
     8161 gcgtttgcag gaaagaagaa accagataac tgctcttttc atgcttcttt cacctccgct
     8221 gcagctgcaa tcagccagcc gcccacccca agagctgcaa gttccactgc tgtaaccagg
     8281 ctctcatttc ctgtcactgc catcttcaca ccttccatca ggatatgggt aggaagaaat
     8341 ggagcagctc tttgtatgct ttccggcaaa aacacttccg gcagaaagcc tcccgaaaga
     8401 aaatgaaatg ccgtcaccag aaaaaactgc agaaaaatgc cgccgatcct acttcctgcg
     8461 gtctgaaaaa gacacacact aaaggatgcc gcccccagac aaacaagaaa tcctgtaaga
     8521 atcgtattta atccaatgga aatcattcct gtccaagcag ctgccgcagc agccagaacg
     8581 gaaatcacgc tcaaaagcac cgatactccc aaaatgcggc ttcccgccgt cgtaaaaggt
     8641 ccgatcccca tcagagacag cttctgcctc ataacagcag gctctctctt taaaaggtca
     8701 gataccgaaa tcacggacaa aagcagcacc agaacaaacg cacttattcc ataaaatact
     8761 ggaacagata catcctttgc cgcatttacc attacttttc tgaaataatc cattcttgga
     8821 agactataag ttaaataaat ctgattcata tcccgctcaa ataccgccat tttatctgta
     8881 agaccgtact cggataaaag ttcatcacca ctgtagattc cggcctgcgc agaacccagt
     8941 atttctgctc ccgcatccgt cagctcctta aaaatgcggc tttctgctcc tgcattttcc
     9001 ggcaggatga cggttaccgg ggtgtttaca ccactcataa tatcctgtat aaacccatcc
     9061 ggcattttaa gaactgcaaa cagctcgccc tttttcagct tttcaccggt cgcttcttcc
     9121 tcaatatagg taaaatcgca gaggcttttt acactttcca gagagctgac catccgcatg
     9181 gcctgccctg ccagcgcatc ttcctcaggc agaacgactc ccaccggaat ccgttccaca
     9241 gtctgtccgc cgtagagcag tttgcctgcc aaaagggcgg ctgcacccat gagtaccaca
     9301 agcaccgccg ctcctgccag aatatgagga agccgtttta atgcccgctt taattccatt
     9361 cctgcatagc acagatatcc acgcatatta tcctgtcctt taatttcctg tatccatctg
     9421 gccgctgaga cttataaagc tgaacagcac ctccataagc acgctgttcc actcttcctc
     9481 tcctgctgcc aatacatcca tggtgtcacc ctctaaagga acaacctcct ccgtcagcgg
     9541 tcttatataa tattcgccgc ttaaaacagc atttcccgtg tctcccattg cagaaacatc
     9601 caaagaatca agagtcaaat gaatattctt tcccttttcc agttcatcca caacaccctg
     9661 agctgaaaaa cctaaaagtt ccattccatc ctgggccgct gctgcctgga catcaaaacc
     9721 gcctccctcg ctgttatagg agctgtcaag ggtaatatcc catttttcag ctatctctcc
     9781 ctgattttct atggaaagct cgaaaccgga tgtgagttct ttcccatcat aggcgccctc
     9841 tttcactgcc tcaatctgta cagatgcagc cccattttcc agcactgcct gagcgctcat
     9901 attctgagtc ggataagcgc ctccctttaa ttcacagcca aactgcagct gaaccggttc
     9961 agcctccgac ccggtactgt taaaggaagt cgttcccttt acagatgcca gacatccttc
    10021 tttatccacg taaaccgtca tgctcacatc atttaacgag ctgtctaaaa atgcgatcat
    10081 cccgtcaatt tccgttctgg cctcctcata ggtctgctcc tgcatctcct gagcggtagg
    10141 gaaatcctgt ccttccatct gcgcacctgc cagctggctt aatttcacgc tcagttccag
    10201 acgctttaag aaatccttct ttaaaacctc atcttgaaga aagaaatcag aggaggttct
    10261 cagaaagttg atcatcgctt ccttgctgac tgtaacctca tatcctctgc aggcctgctc
    10321 ctttccgtct atggtaaaag aagccttctc tcccttttcc acagtgagtg ctgccttaaa
    10381 gtcatcttcc gccttgcagc cttcccggta gcgtttcata agagccttca gatcaaagga
    10441 agtcgcctgc ccctcctgcg tctgtctggc ctgctctgcc aacagctcca tatactctgc
    10501 catcaccgac gcatctactc cactctgctc aagagccgat ccgagcatag gagaattttt
    10561 cagccgttct tccagtcctt ctccaaaatc cagagtaaaa acttttggac tcagttccgg
    10621 cacagcagcc ataactgtat tatccccata ataaagatcc agatttacca gatccattcc
    10681 attgtaaatg attcccatat tggcactgac tttattgttt tccacatcgt ttttcgcatc
    10741 gatccgcagt ccgctccctg cataggtatt taccgcaggc tcagagcagc tgtccagctt
    10801 taacatcagc cccgaattct ggctggcaga aacccttgcc ttcgcaaact ccgacaggcc
    10861 aaacagctcc tccatagggt cggtctgtcc ctctgtataa acattttcaa aggcctggat
    10921 caccgtttcc tttggatcgc cgccggccag ccgggtataa gcaaatactc ctccgccaac
    10981 cactgccact gctgccgctc cgactgctgc cattttcatc ttttttgtca tttccataca
    11041 cgtttcctcc attattaaca gcacacgcca acgtgcgcct ttttgttctt cgccaatcct
    11101 tttatctgtt tgctgccatt aaaaatagtg gatcagttcc ctggagctta atcctgcttc
    11161 aatctctttc agacatctgt ccttcattac atacagacgg tcacaaagcg atagctcttc
    11221 catttcatgg gaagtaagga tcaccgtttt accttcattt acataacctt tcaaatattg
    11281 ccggatggac tccttacact ccagatccaa cgctgctcca ggctcatcca taataaggac
    11341 aggcgcattt cccgacagag cacaggcaat actgaggcgt tttttcattc ctccggacaa
    11401 cttgcccgct ctcatcccca gcatagcagg gattcccaaa agagccgcgg aacccgtttt
    11461 aagatcctca ttcatctgct ttttatttcc cctgtgccac aggaggagat tatctcttac
    11521 cgtaagctct tccataagag gattttcctg cggaacatac gccacattcc gggcaaatac
    11581 cgatggatgg ccaagagcct cttccccgtc aatctttaca cttcccctat cggctttcct
    11641 ggttccggcc agaatagata aaaaggtcgt ttttccgcag ccatttgctc ccacgacccc
    11701 tacgcagctt ccccttccgg caaaaaactc tgcctgctca ataatcctgc gttttccata
    11761 tgatttacag attccggacg cccgcagcat cttcttcccc ccttcgccat cgcacgatgt
    11821 aatttttgtg catttttaac atttaatctg ccagtatctt gaaataagtg taccatattc
    11881 ccccattaca agcaaaatat ttatatttac aaatttctta atgtttttca ccccttgtgt
    11941 ctgcacctgc atttacgctt aaattacgtt tacgaccttg acagtccggt tttgacctgc
    12001 taagataata aacagcgaat tgcttcgttg gcacaccaga atccaatctg aatattcagg
    12061 ggttgtactg gtttcgacgg ggatcatgca gctggtgaag ctatccctat gcgatgggtt
    12121 aaatggcaaa attaaaaata aacgctaacg acgaattagc atacgctgcc taatggcagc
    12181 tgtcagactt aaggcaccca cactttaaga ccctggcatc gactatgtgg gaaacgacgt
    12241 atacaaagct ttgagtatat gggcgtatta tgaagctact aaaaccgtca gagtgtcttt
    12301 tcccgggcgg cggagggaat gttaaataag agactatgat agtagaagaa cagggaattg
    12361 gttttcggac aggggttcga ttcccctcag ctccactttt taaaaaccgc gtaaatgctg
    12421 ggaaagccag tgtttatgcg gttttttcgt ggttttgagg tgtaaggaca ggaagccaga
    12481 aagtaataaa aaacccctgt tttccgggca agtatcacac gaaatatcac acgaaaaaag
    12541 agccggaaaa gcctgattct gtaagggttt cagagccatt gcagagggca aaatatcaca
    12601 cggacagcca aaagacagcg gatagtaata taaaggccgg gaaagcatga gcctccctgg
    12661 cctcttttta tcattctcaa aaataaacgg ctttaccggc agccgtccag aaggtcaagc
    12721 agtatctgcg ccccggctct ggccccttgg gtatatgccg cttcataccc atatagtgac
    12781 gcctccaggt gtgcatctat cagttgatcc agctccggcc tctgctccat ggggagctta
    12841 tctctcaacg cctggtattc cctgtacgct ctttccctgg ctgcctgatg ctctggggtt
    12901 tcttccggct cctgatcggc tggcctgtaa tctccgttgt aaatttgcct gatgatattc
    12961 acgctgtcca tacctcccta tctgtcctct tttgcaaaat gaagttttat gaagtgattt
    13021 ctcctgtgct ctggtttgac tgaggaagat tccccgctca tctctgcaat gcctctctgg
    13081 ctcacggaaa ggctctttcc tgcccttctg tggctttaca tgatattttt attgtgttag
    13141 cctattttgt gtgctatact gtccacacgg aaagtcagag cacataaagg cggcctaccc
    13201 tcccactttt cggaggggct aaccctccag acgaaagaaa ggagggcttg ccaatgtatg
    13261 ttacatattc ggatctgatc cagataggta tcttcattgt atcccttgta ggactgtgtt
    13321 acacaatctt taagggtaga aaatagccgc cactactgcc aatagtgacg gcctaagtta
    13381 aattaagctg ttaattatgg gtaggccgtg tgtttctggc tttccctttt acgtctttac
    13441 tataccatat ctttctgaac cattcaaggg caaaccaatt tttaactttc ctgcgcgtta
    13501 tcgtgtcaat aaattccccg gtaacatcac agccccagcg ccacccgctc tgtgctgctt
    13561 gtccatgtcc ggttgaaatg ctccgcaccg ttgccccaga gggcagagga gtatacccgc
    13621 tcatcctcag gtacgaaccg acgcaacctg ttggagttct ccgggtttct gagcgccctc
    13681 agggccttca tatgctgttt tctggcctct gcctctgtaa tcccacatac ttcccccacg
    13741 cggcccatgg taagccctcc ctggtatcgg ctgcggatca catccggctg tttccccggc
    13801 aggctgtcca cgcactccca caggagagaa cagaggcttt catggtctat tctctctgtg
    13861 gcttcctctt ccagatcaca agcagaggga gccagatctc ccagcgtgat atcttcttcc
    13921 ccttccctgc cccgtacagg cgcgtccagg ctgcacacat ggctcatgca ggcatcaagc
    13981 tgtaatctcc ttacctgatc gacggaaagc cataattcac ggcctatttc agcctcagac
    14041 ggccctctgc cgtgttctaa ctggaaggac aaacaaaacc gcttatatct gctgatagcc
    14101 tcctgacggt ttacaggaag gcgcaggcag ctgccattca cctgcaggta tctctgcatc
    14161 ctctgccgga tccagtactc tgcatagctt aagaatttca ctccctggtc tgcctcatag
    14221 tggtctatgg catcatagag cgcaagaaat ccctcctgct ccagatcctc cagctctccc
    14281 aggcctttat acttccaggc cacagagcgg atgaagtcct ttacctgatc gtaaagctgg
    14341 gccatgttct cgcccacatc ttccccggcc ctgatccggg cggcaagctg ttcgttcgtc
    14401 atagtataaa tcactcctgt tattttctgt ctatgcgccg tgcaatccat tcaccgatca
    14461 gcacgcccgc aacaatggtt atgaactctg taataattcc catctgatac acctcactgc
    14521 atacacgaaa ccggattacg cttcctgatt ccatggaaat aaatgcttta tgcttttaac
    14581 cgtatatttg ccgacagatt ctgaaataac aaggggttcc tgtgtttctt ccaaggggtt
    14641 aaaatctgct gtcgccgcgt ctaaatcaaa cgcgatattt ctcctttgcc catttcccag
    14701 aagaaaaatt ccttcttctg tagggcaagg ggaaagcgta atagtgccgg aattaaaaaa
    14761 ttggatcgca tgaatatgat tcaactgctc ttttaatttc tttatttctg gcaactgtgc
    14821 cgattcaaac cgcctcataa tttcattggc tgcacgtttt tgcattccat cccctaaacg
    14881 catttcacgc aggcgcggcc cgatcattgt tttaaacagg tcaaaatatt ctgtagagcg
    14941 gtattcatca accagatcaa taagctcatg atcttctgaa ccgttgattt ctgcctgcat
    15001 taacgcaatc atattggatt tttttaataa ggctgctgta ctattatcat ctgtgctctg
    15061 gcccttgctg ccagtctcta aagcgttaat ttcctcatcc acaatgtttt taatgcgatt
    15121 gtatgtttct tctttgatct gctgtatata cctcagtgca agctccctgt ttccgggaat
    15181 attacggacg gtcccaaatt cccgtactac ccgcacatac ttgttatcct gttcatgaaa
    15241 ttcatttaat attttttctg tgatcttttc cattttaccg ctcctttacg ttttgatttt
    15301 tcctataccc tttaaataca aaaagacgca ggaacaggag aaaccatttc tggcctatcc
    15361 cattcccgcg tctgtgtttc aagacttccg gcagaggtgc cggggtacta aactatattc
    15421 atctgtgctt attataccaa attgcaaaag gaaaggcaag ggcagcagtt tctttacttc
    15481 ggccaccagg accttctcaa tgtccttttc tctcatgctt tgactacctt cctcacgtac
    15541 acgtgtatgt gtgtgtttta tttatgtgtg tgtatgtatg cgtatgtcct atatttttta
    15601 actctatata ggagtttgta gtcaatgtag tcatatagta aaaatccct
//
LOCUS       DBMDUH010000049        15260 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_2151_length_15260_cov_8.054819, whole genome shotgun sequence.
ACCESSION   DBMDUH010000049 DBMDUH010000000
VERSION     DBMDUH010000049.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 15260)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 15260)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15260
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2151_length_15260_cov_8.054819"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1154)
                     /locus_tag="ACZPCG_05960"
     CDS             complement(<1..1154)
                     /locus_tag="ACZPCG_05960"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HIR1284649.1"
                     /translation="MFVMYEKDAMRLPVKVWLEDKKDLEETCREQAYHLAQLPFLHKW
                     VNLMPDTHAGMGMPIGGVIAAEGVIIPNAVGVDIGCGMAYTETNIPLEAIREIQTGNG
                     TLIQAVIGDIMRNVPVGFAHHKLGMPSYTMDRALEELNLYEEDTELLGQLEAGYFQIG
                     TLGGGNHFIELQEDDQGMLAVMIHSGSRHFGKSVCDYFHYKARQLNQKWYSQVPDEYR
                     LAFLPVDSPEGRQYLRWMQLSMDFAKENREKMMLAVKAILEKYIGKYTELDLKFSGDI
                     NCHHNYASLETHYGKEVWVHRKGAVRAENGELAVIPGAMGSYSYVVMGKGNQESFCSC
                     SHGAGRKYSRKGAMEAFSCEDVILDLRKQGVVLGKKGKADVAEESRFAYK"
     gene            1277..2542
                     /locus_tag="ACZPCG_05965"
     CDS             1277..2542
                     /locus_tag="ACZPCG_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HIR1284650.1"
                     /translation="MAEFQELIKNFDRIRDYMRQFYIYGFKVRNDFDSHCARTYDNER
                     RRIESWLGDYTCSSYTTKGKQVYINIDSKTVPENPLYAAWKSKSFTDNDILLHFFILD
                     QLTDHPQGMNASALSDEISLTYGVAFDPQTVRLKLKEYESLGLLCSHKEGKNLIYQML
                     PPQFPHDSPLNDTLLTAVSFFQGTDPFGFIGSTILDWHDRKNTFFQFKHHFIVHTLED
                     QVLLQSLTAIREHRLIDYENKSFRSGSISSQSGVPLKIFVSTRTGRRYLCLYLPSLRR
                     FHNARLDSILSITIKEPWEDYERTYEALLANIKRCWGVSFGSGRSRLETICLRLSIDE
                     EKEPYVLKRLYREGKGGEILKIREHEYLYTGAFFDTNELLSWVKSFTGRILDIQGTCE
                     PAILKVTRDWERMYQMYCSEEKEETNGTV"
     gene            2529..3536
                     /locus_tag="ACZPCG_05970"
     CDS             2529..3536
                     /locus_tag="ACZPCG_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HIR1284651.1"
                     /translation="MELFNEIYSCYYHTVRHILAEAAGHSVNMKEMEQICKNQAFQET
                     ALAVLPKLTKREWPLLFPSKEHPGCYESALHFSPPELPLTALQMSWLAALIHDPRFRL
                     FFTDEELKTLSESLKGFEPLYQQENFYYYDQFCDGDSYASPSYREHFQAILKALREHH
                     ILFIGYEGKKGICHSFEVTPYQLQYSSKDDKFRLCCLQYRRGRCGLNTILNLERIRDC
                     RVSPDLLSDSDIQTLSCYRFLPVRKAKEPVLLKITGERNSLERCMLHFASYEKHTEYH
                     EEGKYWLCSIYYDTADETELLIDILSFGPVIEVLGPGHFRSMVKNRVKRQHELLYGTV
                     E"
     gene            complement(3627..6239)
                     /gene="adhE"
                     /locus_tag="ACZPCG_05975"
                     /gene_synonym="adhC"
     CDS             complement(3627..6239)
                     /gene="adhE"
                     /locus_tag="ACZPCG_05975"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HIR1284652.1"
                     /translation="MAKKTEQVVPEIIDSVERLNAKMAAMREAQKVFATYTQEQVDKI
                     FFAAASAANKMRIPLAKMAVEETGMGVVEDKVIKNNYAAEYIYNAYKNTKTVGVVEED
                     KEYGIKKIAEPIGLIAAVIPTTNPTSTAIFKTLISLKTRNAIIISPHPRAKKSTIEAA
                     KVVLEAAVKAGAPEGIIGWIDVPSLELTNEVMRDADIILATGGPGMVKAAYSSGKPAL
                     GVGAGNTPVIIDDTADIRMAVNSIIHSKTFDNGMICASEQSVTVLEPVYEAVKKEFEY
                     RGCYFLKQGEIEKVRKTILINGALNAKIVGQSAYTIAKLAGVDVPVDTKIIIGEVESV
                     DISEEFAHEKLSPVLAMYKAKTFDEALDKAERLVADGGYGHTSSLYINVNEKEKMMKH
                     AERMKTCRILVNTPSSHGGIGDLYNFKLAPSLTLGCGSWGGNSVSENVGVKHLLNIKT
                     VAERRENMLWFRTPEKVYFKKGCLPVALNELKDVMGKKRAFIVTDSFLYKNGYTNVIT
                     DRLNEMGITYTVFSDVQPDPTLANAQAGAKLMKEFEPDVIIAMGGGSAMDAGKIMWVL
                     YEHPEADFMDMAMRFIDIRKRVYTFPKMGEKAYFIAVPTSSGTGSEVTPFAVITDQET
                     GIKYPLADYQLLPNMAIVDTDNMMSQPRGLTSASGVDVLTHALEAYASVMATDYTDGL
                     ALKAMKNVFDYLPIAYNEPNNVEARQKMADASCMAGMAFANAFLGVCHSMAHKLGAFH
                     HLPHGIANALMISLVVEFNAAENPRKMGTFSQYQYPHTMARYAECARFCGINAGSDEE
                     AVKKLIEKIEELKKAVGVKSCIKDYGIDEKDFLDRLDDMTEQAFDDQCTGANPRYPLM
                     SEIKDMYLRAYYGK"
     gene            6558..7034
                     /locus_tag="ACZPCG_05980"
     CDS             6558..7034
                     /locus_tag="ACZPCG_05980"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR1284653.1"
                     /translation="MFRPMRRCGQQLKLNECIEILKAEPRGVLSLLGDNGYPYGIPMD
                     HWYCEQDGKLYFHGAKNGHKIDSLQSCDKVSYCVYDKGFRKEGEWALNIRSVIVFGRM
                     HPVADTRQAETICRCLCRKFTDDSDYIDHEIEHFLSNVLCLELTPEHITGKLVNES"
     gene            complement(7098..8285)
                     /gene="metK"
                     /locus_tag="ACZPCG_05985"
     CDS             complement(7098..8285)
                     /gene="metK"
                     /locus_tag="ACZPCG_05985"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018595377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HIR1284654.1"
                     /translation="MERRLFTSESVTEGHPDKMCDAISDAILDALMEQDPMSRVACET
                     ATTTGLVLVMGEITTKAYVDIQKIVRETVREIGYDRAKYGFDCDTCGVITAIDEQSSD
                     IAMGVDKALEAKENRMSEEELDAIGAGDQGMMFGYASNETEEYMPYPISMAHKLARRL
                     TEVRKNGTLKYLRPDGKTQVTVEYDENGKPVRLDAVVLSTQHDENVTQEQIHADIKKY
                     VFDEIIPADMVDENTKFFINPTGRFVIGGPHGDSGLTGRKIIVDSYGGTARHGGGAFS
                     GKDCTKVDRSAAYAARYVAKNIVAAGLADKCEIQLSYAIGVAHPTSIMVETFGTGKLS
                     EEKLVEIIRENFDLRPAGIIRMLDLRRPIYKQTAAYGHFGRNDLDLPWEKLDKVDTLK
                     KYL"
     regulatory      complement(8471..8609)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(8754..10610)
                     /locus_tag="ACZPCG_05990"
     CDS             complement(8754..10610)
                     /locus_tag="ACZPCG_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009295767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HIR1284655.1"
                     /translation="MTGRTGKRRFLGLLWSLILTCSLITGMSMSAWAQEAGTVKGADQ
                     DIVVLYTNDVHCAIDENIGYGGLALYKKQMMNQTPYVTLVDAGDAIQGAPVGTLSEGG
                     YIIDIMNQTGYDVAVPGNHEFDYGMPSFLELAGKLNCGYYSCNFISTASGQSVFAPYK
                     MISYGDTQVAYVGVSTPESFTKSTPAYFQDANGNYIYSFCEDADGTAFYNQVQSAVDS
                     ARAEGAEFVILVGHLGENGITERWSAGSLIGHIRGVDACIDGHSHETTLSMIVKDADG
                     KDVIVTQTGTKLANIGKMTIHTDGTISTELISEVVPSEVNRTYTVQKNDSLSRIAKRE
                     LGSYDRWREIYDSNRDKISDPGTIYPGMVLTIRQGSVVNENGRAVDPDTHSLIEQIKG
                     QFNETLKTPLGSTAYKLTIKDEATGERRIRNGETNLGDLAADAYRVQLGADIGLCNGG
                     GVRTDIESGTITYEDTLAVFPFGNMGCVAEVTGQQIKDALEMGAKNYPEESGGFMHVS
                     GLTYTIDSSVKSSIKVDDKGNFVSVDGPYRVTDILVGGQPIDLNKTYTVASHNYMLKS
                     GGDGMTMFNGCNIIKDEVMVDVDVLSTYIQGLGGSVSSEYENSEGQGRIVIR"
     gene            complement(10706..11998)
                     /locus_tag="ACZPCG_05995"
     CDS             complement(10706..11998)
                     /locus_tag="ACZPCG_05995"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIR1284656.1"
                     /translation="MEHYIIKGGNPLVGDVTIGGAKNAALGILAASIMTDEDVLIDNL
                     PDVRDINVMLEAIQEIGASVHRIDRHTVKINAGNIREVSVDDEYIRRIRASYYFIGAL
                     LGKYKSAQVPLPGGCNIGSRPIDQHIKGFRALGADVQIEGGAVIAHAIDLVASHIYLD
                     VVSVGATINIMMAAAMAEGQTILENAAKEPHIVDVANFLNSMGANIKGAGTDTIRIRG
                     VERLHGTEYSIIPDQIEAGTFMCAAAATRGDVMVKNVIPKHLEAISAKLTEIGCEVIE
                     FDDAVRVVGKPNQRHTDIKTLPYPGFPTDMQPQMAVTLALTKGTSMVTESIFENRFKY
                     VDELARMGSSIKVEGNVAVINGVEGFTGAQVNAPDLRAGAALVIAGLAAEGYTIVDEI
                     GYIQRGYESFEEKLQGLGAMIELVDSEREMQKFKLRVG"
     gene            complement(12165..14645)
                     /locus_tag="ACZPCG_06000"
     CDS             complement(12165..14645)
                     /locus_tag="ACZPCG_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284657.1"
                     /translation="MKKIIQRAGILFVVFLAALVVYFIGAHRTSEQEGAVYSSMDESR
                     LPVVYTEFEGKEINGLHGYVQDMGNRAAGEAISVLSSDRRLNLRIHEFGNTITEISYE
                     IRNLSMDRLIERTELSDWSSKEGITTVSLPIQNLISKDKAYLLILNVKTAEQQIQYYT
                     RIMWTDAPRALDMLQLAEEFTRKSLDYEQAKELVSYLETSPEEDNSSLGHVTIRASFD
                     HLTWDGLHAEMEGEPRITLQEFDGIMGQVQVQYYVRLTDSQGKESLAEAEDNFAMKWN
                     EQRIYLMNYERNANHIFTGSEGAFSGKRIVLGISDEAGIGTVKSQDSRYILFKVNGNL
                     WRYDQSDHRIMCVFTFSNGETDDVRSSYGDHDVQILRAGDDGSADFLVYGYMNRGPYE
                     GQTGVIFYRYDAGSNTVQEKFFIPVVQEFEKLKKDIEQLAYVSPGEMVYLMLNGNVFG
                     IDLSSNESISVAQGLTEGTFAVSHDGSRMAWQEKREDTRDERIHVMDFNTSQKQEIPV
                     SEGDYARVLGFVGSDLIYGFARQEDVWSVNGRVQEIPMYVMYIADSEMNIESEYRKDG
                     IYISDVVTEDGRIHLKRLVKLGDNQYSYQDEDTIVCNEKVETDPLEGIGWYASSEMGR
                     MYFVQMDSDQKASSVKSERPKAFSYENTGTLELAGKAASGEKGEVIFYAYGGGHFLGA
                     SRQFAEAVNLAYDKMGFVTDERGHLVWDRINRQPIRNLKDPSGAAGAVMSHLDGFTRS
                     TLYEDGIMVIDATGCTLNQVLYFIDKGIPAAAYREDGSYVLLSGYDTYNVTIYDPAVQ
                     ENVKMGLGDAAAHFGSQHNRFLCGLKIE"
BASE COUNT         3824 a         4112 c         3064 g         4260 t
ORIGIN      
        1 tctttatagg caaaccggct ttcctctgcc acatctgcct ttcccttctt tcccagaacc
       61 acgccctgtt ttctcagatc caggattaca tcctcacagg aaaaggcttc catagcgcct
      121 tttctggaat atttccgccc tgctccgtga gaacaggaac aaaaactctc ctgattcccc
      181 tttcccatca ccacatagct gtaagaaccc atggcgccgg ggataaccgc cagttcgccg
      241 ttttctgccc ttaccgcgcc ttttctgtgc acccatacct cttttccata gtgtgtttcg
      301 agggatgcat aattatgatg acagttaata tcgccggaaa acttcaaatc cagctccgta
      361 tattttccaa tatacttttc caggatcgcc tttactgcca gcatcatctt ttcccggttt
      421 tcctttgcaa aatccatgga aagctgcatc cagcgcagat actggcggcc ctccgggctg
      481 tccacgggca gaaaagccag cctgtattca tccggcacct ggctgtacca tttctggttc
      541 agctgacggg ccttataatg aaaatagtcg cagacggatt ttccaaaatg acggctgccg
      601 gagtgaatca tcactgccag catcccctga tcatcctcct gaagctcaat aaaatgattt
      661 cctccgccca gagttccgat ctgaaaatat ccggcctcca gctgtcccaa aagctctgta
      721 tcctcctcat acaggttcag ctcttccagt gcccggtcca tggtataaga gggcatcccc
      781 agcttatgat gggcaaagcc cacgggaaca tttctcataa tatcaccgat caccgcctgg
      841 atcagcgttc cgttcccggt ctggatctcc cgaatggcct ctaacggaat attggtttcc
      901 gtatatgcca tcccgcagcc aatatccaca cccactgcat tagggatgat cactccctct
      961 gcagcgatca caccgccgat gggcattccc attcctgcat gggtatccgg catcagattg
     1021 acccatttat ggagaaatgg aagctgtgcc aggtggtatg cctgttccct gcaggtctct
     1081 tccaaatcct ttttatcctc aagccatacc tttaccggca atctcatggc atccttttca
     1141 tacattacga acatatcctg cacatccttt catttcttat catggcttta tcttagcctg
     1201 attttccatt tttatcattt aaaaaaaggt gcgaacccgc tataatggag ctatatcacc
     1261 ctatggaggg atctatatgg cagaatttca ggagcttatc aaaaactttg accggatccg
     1321 ggattatatg cgccagtttt atatttatgg atttaaggtg agaaatgatt ttgactccca
     1381 ctgcgcccgg acctatgaca acgaacggag gcggatcgag agctggctgg gcgattacac
     1441 ctgctcttcc tatacgacca agggaaaaca ggtctacatc aatatcgaca gtaagacagt
     1501 gcccgaaaat cccctctatg cggcctggaa atcgaaaagc tttacggata atgatatcct
     1561 tctgcacttt ttcattctgg atcagctcac cgatcatcct cagggaatga atgcctctgc
     1621 cctgagtgat gaaatttccc ttacctacgg cgttgccttt gacccccaga ccgtgcggct
     1681 gaagctgaag gaatacgaat ctctgggact gctgtgctct cataaagaag gaaaaaacct
     1741 tatctatcag atgctcccgc cccagtttcc ccatgactct cccttaaatg atactctttt
     1801 aaccgctgtt tccttttttc agggaaccga ccctttcggg tttatcggga gcaccattct
     1861 ggactggcac gaccggaaaa ataccttttt tcagttcaag catcatttta tcgtacatac
     1921 cctggaagat caggtgctcc tgcaaagcct tacagccatc cgggagcacc gtttgatcga
     1981 ctatgaaaat aagagtttcc gttccgggag catttcctcc cagtccggcg ttcccttaaa
     2041 aatctttgta agcacccgta ccggaaggcg ctacctctgt ctgtaccttc catctctgcg
     2101 gaggttccac aatgcccgtc tggattccat tctctccatt accattaagg agccctggga
     2161 agactatgaa cgaacctatg aggcgctgct ggcaaatata aagcgctgct ggggcgtttc
     2221 cttcggaagc ggaagaagcc gcctggaaac catctgcctg cgcctttcta ttgatgaaga
     2281 aaaagaaccc tatgttttaa aacgccttta ccgggagggg aaaggcggag aaatcctaaa
     2341 aatacgggag catgaatatc tgtatacggg agcatttttc gataccaatg aacttctgtc
     2401 ctgggtaaag tcctttaccg ggcgcattct ggatattcag ggaacctgtg aacctgccat
     2461 ccttaaggtc acaagagact gggaacggat gtatcagatg tactgcagcg aagaaaagga
     2521 agaaacaaat ggaactgttt aatgagatat acagctgtta ctaccacacg gtccgccata
     2581 ttttggcaga agctgccggc cattcagtga acatgaaaga aatggagcag atctgcaaaa
     2641 atcaggcctt tcaggaaaca gccctggctg tactcccgaa acttacaaag agagagtggc
     2701 cccttctttt cccctcaaag gagcatcctg gctgctatga aagtgccctg catttttctc
     2761 ctcccgaact gccgctgact gcacttcaga tgtcctggct ggcagctctg atccatgacc
     2821 cgcgcttccg gctctttttt acggatgaag aattaaaaac tctttcagaa tccctaaagg
     2881 gatttgaacc gctgtatcag caagaaaatt tttactatta cgaccagttt tgcgacggtg
     2941 attcctatgc ttcaccttcc taccgggagc attttcaggc aattttaaaa gccctgaggg
     3001 agcatcatat cctttttatt ggatatgaag gcaaaaaagg aatctgccat tcctttgaag
     3061 tcacccccta tcagctccag tactcctcca aggatgacaa attccgtctc tgctgtctgc
     3121 agtaccgacg gggacgctgc ggcctaaata ccattctcaa cctggaacgg atccgtgact
     3181 gccgcgtttc ccctgacctc ctttcggaca gcgatataca gactctctcc tgctaccgct
     3241 ttctcccggt ccgcaaagca aaagagcccg ttctcttaaa aataaccgga gaacggaact
     3301 ctcttgaacg ctgtatgctt cactttgcca gctacgaaaa gcatacggaa taccatgaag
     3361 aaggcaaata ctggctgtgc tccatttact atgataccgc agatgaaacg gagcttctca
     3421 tcgatatcct ttccttcggc cccgtaatag aggttctggg gccaggccat ttccgcagta
     3481 tggtgaaaaa ccgggtgaaa cggcagcatg agcttttgta tgggacagtg gagtaaatat
     3541 ttctacgcga acaggccgcc tgccctaacc ggacagacgg cctgttccaa acacaaagga
     3601 taaaggttca ccctcaatgg gttcgattat tttccgtaat aagctcttaa atacatatcc
     3661 ttgatctcgc tcatgagagg atatcttggg ttggcaccgg tacactggtc gtcgaaggcc
     3721 tgctccgtca tatcatccag tctgtctaag aagtctttct cgtcaatgcc gtaatccttg
     3781 atgcaggact tcacgccaac agccttcttt aactcctcga tcttctcgat cagcttcttc
     3841 acagcctctt catcgctgcc tgcattgatt ccgcagaatc ttgcacactc agcgtatctt
     3901 gccatggtat gcggatactg gtactgggag aaagttccca tcttgcgtgg attctcagca
     3961 gcgttgaact caactaccag agagatcatc agcgcatttg caataccgtg gggcagatgg
     4021 tggaatgctc ccagcttatg tgccatagag tggcatacgc ccaggaatgc gttcgcaaat
     4081 gccatacctg ccatacagga tgcgtctgcc atcttctggc gtgcctcaac attgtttggc
     4141 tcattgtaag cgatcggcag gtagtcaaat acgttcttca ttgcctttaa tgcaagtccg
     4201 tctgtataat ccgttgccat aacagaagca tatgcctcta atgcatgggt taaaacatca
     4261 acgccggaag cgctggttaa gcctcttggc tggctcatca tgttgtcagt atcaacaatc
     4321 gccatatttg gaagcagctg gtaatcagcc agaggatact tgatgccggt ttcctgatca
     4381 gtgataacgg caaatggagt aacctcagaa cctgtaccgg aagaggtagg aacagctatg
     4441 aaataagcct tctcacccat cttcgggaag gtataaactc tcttgcggat atcgataaag
     4501 cgcattgcca tatccatgaa atctgcttcc ggatgctcgt acagtaccca catgatcttt
     4561 cctgcatcca ttgcggagcc gccgcccatt gcgataatta catccggctc aaattctttc
     4621 atcagtttgg cgcctgcctg tgcatttgcc agagtaggat caggctgaac gtcagagaat
     4681 acggtatatg tgattcccat ttcatttaaa cggtctgtaa ttacatttgt gtagccgttc
     4741 ttgtacagga aggagtctgt tacgataaag gctctcttct tgcccattac atcctttaat
     4801 tcgtttaatg ctacaggcag acagcccttc ttaaagtaaa ccttctcagg tgtacggaac
     4861 cacagcatat tttctctcct ctcagcaaca gtcttaatgt tcagcagatg cttaacgcca
     4921 acgttctcag atacggagtt tccgccccag gaaccgcagc ccagtgtaag agacggagcc
     4981 agcttaaagt tgtacagatc gccgataccg ccgtgggagg aaggtgtatt tacaagaata
     5041 cggcaggtct tcattctttc tgcatgcttc atcatctttt ctttttcatt tacattgatg
     5101 tacagagaag aggtatggcc gtagccgccg tctgctacca gacgctctgc cttatccaga
     5161 gcctcgtcaa aggtcttagc cttgtacata gccagtacag gagacagctt ttcgtgagca
     5221 aactcctcgc tgatatctac agactcaacc tcgccgatga taatctttgt atcaaccgga
     5281 acatccacac ctgccagctt tgcgatggtg tatgcagact gtcctacgat ctttgcattt
     5341 aatgccccgt tgatcaggat ggtctttctt accttttcaa tctcaccctg ctttaagaaa
     5401 tagcagcctc tgtattcaaa ttccttctta acagcctcat atacaggctc cagaacagtt
     5461 acagactgct cagaagcaca gatcatacca ttatcaaagg tcttggaatg gataatggag
     5521 ttaactgcca tacggatatc agccgtatcg tcaatgataa ccggcgtatt tccagcgcca
     5581 acgcccagcg caggcttacc ggaagaatat gcagccttaa ccatgcccgg gccaccagtt
     5641 gccaggatga tatcagcgtc tctcattacc tcattggtca gctccaggga aggaacgtcg
     5701 atccatccaa tgatcccctc aggagcgcct gccttaacag cagcctccag cacaaccttg
     5761 gcagcctcaa tggtactctt ctttgctctt ggatgtgggg aaatgatgat ggcattgcgt
     5821 gtctttaagg aaatcagtgt cttaaagatt gcagtggagg ttggatttgt agtagggata
     5881 acagctgcga tcaggccgat tggctctgca atcttcttta taccgtactc cttatcctcc
     5941 tcaaccacac caacggtctt tgtattcttg tatgcattgt agatatactc agctgcgtag
     6001 ttgttcttga ttactttatc ctcaaccacg cccattccgg tctcttctac cgccatcttt
     6061 gccagcggaa tacgcatctt attcgctgcg cttgctgccg cgaaaaagat cttatctacc
     6121 tgctcctgtg tgtaggttgc aaataccttc tgagcctcgc gcattgctgc catcttggcg
     6181 ttcagtctct ctacgctgtc aatgatctcc ggaactacct gctcggtctt ctttgccatt
     6241 tccatttcct ccattttcct tgtgtttgtg tttgtattcg gaatgtttgt caatttatta
     6301 acaatttaac tatccgcatt atataacatc gttaaaaaaa tgtcaaccat attttttctt
     6361 ttttcaggtt ttgcgcaaat ttttagtctc agagcatact ctatcgattc accaatggca
     6421 aaaagattgt taaatatata acacaacttt ggatgttttg tataatttta aaacctatcc
     6481 actgctattt tataaattat ggtaaaatat acctggcaac tataaaaata aaactgtaac
     6541 ataaggagtg attttctatg tttcgaccaa tgcgacgatg cggacagcag ctgaagctga
     6601 acgaatgtat tgaaattctg aaagcagagc ccagaggagt gctctctctt ctgggagata
     6661 acggatatcc gtacggtatc cccatggatc actggtactg cgaacaagac ggcaaactct
     6721 attttcacgg tgcaaaaaat ggccataaaa ttgattcctt acagagctgt gataaggttt
     6781 cctattgtgt atatgacaaa ggcttccgta aagaaggaga atgggctctg aatatccgaa
     6841 gcgttattgt attcgggcga atgcatccgg tcgcagacac ccggcaggca gaaaccatct
     6901 gccgctgcct ctgccggaaa ttcactgatg attcagacta tatcgaccat gagatcgaac
     6961 attttctttc caatgtactc tgtctggagc taactccgga gcacataacc ggaaagcttg
     7021 taaatgaatc ttaactgtta aaaaaggcat ctgcacgccg atacatacca ttgtgcaaat
     7081 gcctttttgt attcttatta cagatatttt ttaagggtat ccaccttatc taacttctcc
     7141 cagggaaggt caaggtcatt gcggccaaaa tgtccgtaag cagcggtctg cttgtagatc
     7201 ggacgacgca gatccagcat acggatgatt ccagccggac gaaggtcaaa gttctcgcgg
     7261 atgatctcta ccagcttctc ctcactgagc tttcctgttc cgaaggtctc taccataata
     7321 gaggttgggt gagccacgcc gattgcatag gacagctgga tctcgcactt atcagccaga
     7381 ccggcagcta cgatattctt agccacatag cgggccgcat aagctgcaga acggtctacc
     7441 ttggtgcagt ccttaccgga aaatgcgccg ccgccgtgac gtgccgttcc gccgtaggaa
     7501 tctacaataa tcttgcgtcc ggtcagaccg ctgtctccgt gaggtccgcc gattacaaag
     7561 cgtcctgtag gattgataaa gaactttgta ttctcatcta ccatatctgc aggaataatc
     7621 tcatcaaata catacttctt gatatctgca tggatctgct cctgagttac attctcatca
     7681 tgctgagtgg acagaaccac tgcatccaga cgaaccggct ttccattctc gtcatactct
     7741 actgtaacct gagtctttcc gtccggacga agatacttta aggtaccgtt ctttcttacc
     7801 tcagtcagtc ttctagccag cttgtgagcc atggaaatag gatatggcat atactcctct
     7861 gtctcgttgc ttgcatagcc gaacatcata ccctggtcgc ctgcaccaat ggcgtccaac
     7921 tcttcctcgg acatacggtt ttcctttgcc tcaagagcct tatcaacacc catagcaatg
     7981 tcggaggact gctcgtcaat ggcggtaatc acgccgcagg tatcacagtc aaaaccatat
     8041 tttgcacggt catagccaat ctcgcgaacc gtctctctta cgatcttctg gatatctaca
     8101 tatgcctttg tcgtgatctc gcccataacc agcaccagtc ctgtggtggt cgctgtctcg
     8161 caggcaacac ggctcatggg atcctgctcc attaaagcgt ctaaaatcgc atcagaaatc
     8221 gcgtcgcaca ttttgtccgg atggccttca gttacagatt cagatgtaaa taatcttctc
     8281 tccatcaatc attctccttt tctttgctgt aaaataaata catactgtac tgtgtttcgt
     8341 gtcatttaag caggtgtaat cctctgccct ctggcaaaaa gaaaagtccg cagcaacggc
     8401 ggacttgaaa atatcagatc ttcaaatcct cttattgctc gatcaagcac atcaaaaagc
     8461 tctttttaaa actttataag ctgcactcct cgctcaggct ggcaccttcc ggccctttcg
     8521 cacaccgcat aagcactgct tacgggatac tcaaccgggg ttgccgtgac ttcacaggtc
     8581 ctttatacct ccatcactct gaataaggga tatttacgaa cgttttctat tgtgaaatga
     8641 ctactagcat atattaaatc attttttctt tcctgtcaat atacattttt ccgcgcaaaa
     8701 aatcccggca aagttccatc tcctctgccg gaattttcat taaaacaatc tttttaccgg
     8761 atcacaatcc gtccctgtcc ttcggaattc tcatattcag aggatacaga accgcccagg
     8821 ccctgaatat aggtggataa tacatctaca tccaccataa cctcatcctt tataatattg
     8881 cagccattaa acatggtcat gccgtcccca ccggacttta acatataatt atgggaagcc
     8941 actgtgtaag tcttattcag atcaatgggc tgtccgccaa ccagaatatc cgtaactctg
     9001 tacggcccgt ctacactgac aaaattaccc ttatcatcca cctttatact ggattttaca
     9061 gagctgtcaa tggtataggt cagaccggat acatgcatga agcctccgga ctcctcagga
     9121 taattcttag cgcccatttc cagggcatcc ttgatctgct gtcctgtcac ctctgccaca
     9181 catcccatgt ttccaaatgg gaatacagcc agtgtatcct catatgtaat ggttccggac
     9241 tcaatatctg tgcggacgcc tccgccattg caaagtccga tgtctgctcc cagctgaact
     9301 ctgtaagcat ctgccgccag atcaccaaga ttagtttcgc catttcttat ccggcgttct
     9361 ccggtggcct catccttgat cgtcagcttg tacgctgtac ttcccaatgg ggtctttaag
     9421 gtttcgttaa actgcccctt gatctgctcg atcaggctgt gggtatccgg atccactgca
     9481 cggccatttt catttacaac actgccctgg cggatggtaa gaaccattcc gggataaatg
     9541 gtacccggat cagaaatctt atcccgattg ctgtcataaa tctctctcca gcggtcatag
     9601 cttcccagct ctctcttggc gatcctgctt aaggaatcat tcttctgaac cgtataggta
     9661 cgatttactt ctgaaggcac aacctctgag atcagctctg tggaaatcgt tccatctgta
     9721 tgaatggtca tctttccaat atttgccagc ttagtgcctg tctgggtaac gataacatcc
     9781 tttccgtctg catcctttac tatcatggat agtgtagttt catgggaatg tccgtcaatg
     9841 caggcatcca cgccccggat atgaccgatc aggcttccgg cactccagcg ctctgtgatt
     9901 ccattttctc ccagatgtcc aactaaaatc acaaattccg ctccttcagc cctggcactg
     9961 tccactgccg actgcacctg attgtaaaat gcagttccgt ctgcatcctc acagaaggaa
    10021 tagatataat ttccattcgc atcctggaaa taagcaggcg tagattttgt aaagctttcc
    10081 ggtgtggaaa cgcccacata agccacctgt gtatcaccgt aagaaatcat cttatagggt
    10141 gcaaatacag actgtccgga agcagtgctg ataaagttgc aggaataata tccgcagttt
    10201 aattttcctg ccaattccag aaagctgggc ataccgtagt caaactcatg atttcccgga
    10261 actgccacat catatcctgt ctgattcatg atatcaatga tataaccgcc ctcggacaga
    10321 gttcctaccg gagcaccctg gatggcatca cctgcatcca ccagagtaac atatggtgtc
    10381 tggttcatca tttgcttttt gtacagcgcc aatccgccgt aaccgatgtt ttcatcaatt
    10441 gcgcagtgaa catcattggt atatagaacc acaatgtcct gatccgctcc tttgactgtt
    10501 ccagcctcct gtgcccatgc ggacatggac atacctgtga taaggctgca ggtaagaatc
    10561 agggaccaaa gcagacccag gaaccttctt tttcctgttc tcccggtcat ttcataaccc
    10621 tccttatgag ataattttgc ctctgcccgg attactcctg acagaggcta ttcttatatg
    10681 aaaccgtacg atggatatac ggtcatcatc ctaccctaag tttaaatttc tgcatctccc
    10741 tttcagaatc caccagctcg atcatggctc ccagcccctg gagcttttcc tcaaagcttt
    10801 cgtatcccct ctgaatatat ccgatctcat ctacaatggt atatccttct gccgccagcc
    10861 ctgcgattac cagagctgca cctgcccgaa gatccggcgc atttacctga gcgccagtaa
    10921 agccttctac gccgttaatc acagctacat ttccctctac cttgatagag cttcccatgc
    10981 gtgccaactc gtccacgtat ttaaaacgat tctcaaaaat gctttctgta accatactgg
    11041 ttcctttggt aagagccagt gtaacagcca tctgaggctg catatcggta ggaaagccgg
    11101 gatatggcag tgtcttaata tctgtatgac gctggttcgg cttacccaca acacggactg
    11161 cgtcatcaaa ctcgatcacc tcacagccaa tctctgtaag cttggcggaa atcgcctcca
    11221 gatgctttgg aattacattt tttaccatca catcgcctct ggttgctgct gctgcacaca
    11281 taaaggtacc agcctcgatc tgatccggaa taatagaata ttctgtgccg tgaagccgct
    11341 ctacgcccct gatacggatt gtgtctgttc ctgcaccctt aatattggct cccatactgt
    11401 taaggaagtt tgccacatct acgatatggg gctccttggc agcattctca agaatcgtct
    11461 gtccctctgc catagcagca gccatcatga tattgatggt cgctcccaca gaaaccacat
    11521 ccagataaat atgacttgcc accaaatcaa tggcatgggc gatcactgct ccgccctcaa
    11581 tctgtacatc tgctcccagc gcccggaagc ccttaatatg ctggtcaatg gggcggcttc
    11641 cgatattgca gcctccggga agaggaacct gagcgctctt gtattttcct aaaagagcac
    11701 cgataaaata ataggacgca cggatccggc ggatatattc gtcatccacg gatacttctc
    11761 tgatatttcc tgcgttgatc tttactgtat ggcggtcaat gcggtgtacg ctggcaccga
    11821 tctcctggat tgcttccagc ataacattga tatcccgcac atccggcaga ttgtcgataa
    11881 gaacatcctc atcggtcata atggatgctg ccagaatacc aagtgcggca tttttggctc
    11941 cgcctatggt cacatcgccc accagcggat tgccgccttt tataatataa tgttccattt
    12001 tatacctcaa ttactgatcg aacagacctt aatttttctt tctgtctttg ttcacatttt
    12061 atttacatct cttagattat accataagaa caggatttgt aaagcaaact cgcctgccgg
    12121 gcgaaaaaaa cgtttcaccg gacaggcgtg tttcaaaatt atttttattc aatttttaac
    12181 ccgcacagga aacggttatg ctggcttcca aagtgcgcgg ctgcgtcccc cagccccatt
    12241 tttacgtttt cctggacagc aggatcataa atggttacat tataggtatc ataaccggac
    12301 agaagaacat aacttccatc ctctctgtaa gcggctgccg gaatcccttt atcaataaaa
    12361 tacaatacct gattcagtgt acagcccgta gcgtcaatca ccatgatccc gtcctcatac
    12421 agcgtacttc tggtaaatcc atccagatgg ctcatcactg caccggctgc accggatgga
    12481 tctttcagat ttcggatcgg ctgacggtta atgcgatccc agacaaggtg tcctctttca
    12541 tctgtcacaa atcccatttt atcataggca aggttcacag cttctgcaaa ctgtctggat
    12601 gctcccagaa aatggccgcc tccgtaagcg taaaaaatca cttcaccttt ttctcccgaa
    12661 gccgcttttc ctgccagctc cagagtacct gtattttcat acgaaaatgc ctttggccgt
    12721 tcggatttta cagacgaagc cttctggtct gaatccatct gtacaaaata cattcgtccc
    12781 atctcggaag aagcatacca gccaattccc tccagtggat ctgtttccac cttttcattg
    12841 cagacaatgg tatcctcatc ctgataggaa tactgattgt cccccagctt taccagcctc
    12901 tttaaatgaa tccgtccatc ctccgtcacc acatctgaaa tgtagattcc gtcctttctg
    12961 tattcgcttt caatgttcat ctcactgtct gcgatataca tcacatacat ggggatttcc
    13021 tgcacccttc cgtttacact ccagacatcc tcctgccggg caaaaccata gatcagatca
    13081 cttcctacaa agcccagaac cctggcataa tcaccttctg aaacaggaat ttcctgtttt
    13141 tgggatgtat taaaatccat cacatgaatc ctctcatccc gggtgtcctc tctcttttcc
    13201 tgccatgcca tgcggcttcc gtcatggctt accgcaaagg ttccctccgt aagtccctga
    13261 gctacagaaa tggattcgtt actgcttaaa tcaattccaa atacatttcc attcagcatc
    13321 agatatacca tttctccggg acttacatag gccagctgct ctatatcctt ttttaatttt
    13381 tcaaattcct gaacaaccgg aatgaagaat ttctcctgga ctgtattgct tcccgcatca
    13441 tagcgataaa atatcactcc ggtttgaccc tcatacggtc cacggttcat atagccgtaa
    13501 accagaaaat ctgccgagcc gtcatctcct gctctcaaaa tctgtacatc atgatctcca
    13561 tagctgctgc gcacatcgtc tgtttctcca tttgaaaatg taaacacaca catgatcctg
    13621 tgatctgatt gatcataacg ccacagattg ccatttactt taaaaagaat ataccgggaa
    13681 tcctggctct ttaccgtacc aataccggct tcatcggaaa ttcccaaaac aatcctcttt
    13741 ccggaaaatg ccccttcact tcctgtaaaa atatggttgg cattgcgctc ataattcatt
    13801 aagtagatcc gctgctcatt ccatttcatg gcaaaattat cttctgcctc tgccagactc
    13861 tcctttccct gcgaatctgt aagccggaca taatattgaa cctgaacctg tcccataatc
    13921 ccgtcaaatt cctgaagggt aatcctgggt tctccttcca tctccgcatg gagtccatcc
    13981 caggtcaaat gatcaaaact ggcccggatc gtcacatggc ccagagaact gttatcctcc
    14041 tccggactgg tttccagata cgataccaat tcctttgcct gctcataatc caaactcttt
    14101 cttgtaaatt cctctgccag ctgcagcata tccaaagccc ttggagcatc cgtccacatg
    14161 atacgggtat aatactggat ctgctgttct gctgtcttta catttaaaat taagagatac
    14221 gctttatcct tagaaatcag attctggata ggaaggctca ctgtggttat gccctccttg
    14281 ctggaccagt ccgaaagctc tgtccgttca atcaggcgat ccatggaaag attccttatc
    14341 tcataactga tttctgtaat tgtatttcca aactcatgga tgcgaagatt cagtcttctg
    14401 tctgaagaca aaacagaaat cgcttctccg gccgcacgat ttcccatatc ctgaacatat
    14461 ccgtgcagac cgttaatctc ctttccttca aactccgtat ataccaccgg caatcttgat
    14521 tcatccatgg atgaatatac ggcaccctcc tgctctgatg tcctgtgtgc accgataaag
    14581 tataccacca atgcagccag aaaaaccaca aacaggattc ctgctctctg tattattttt
    14641 ttcatctgtt cattcctttt cagaatttta tctgctcaat tttacctttc catctccggt
    14701 gtgagaactg gaagaaaaat ccgcttacaa gcaagcttga cacatatttt tctgccagtt
    14761 ctccctccgt cctgaatcgt tacctctatt ttatataata accgttaaaa ctacaactta
    14821 atcttttttg aatatttcct gtgataactt ttatgctgta attctatcca atacacattc
    14881 ctctgcggca ccgccggttc tgcatctcat ctaaaatcag ggtctttaca atatcgcgaa
    14941 accaggctgg ataaatacca ggtctgggtg gacgtaccgg accgggaccc tgcggtggat
    15001 acggtggacg cactgggccg ggaccctgcg gcggatacgg tggacgcacc gggccgggac
    15061 cctgcggcgg atacggtgga cgcaccgggc cgggaccctg cggcggatac ggtggacgca
    15121 ctgggccggg accctgcggc cgggctgatc ccggaatatg gggcggacgt ctgagttcct
    15181 gtcccagtag caccgggcca ttttcagttt ccacaggaac aaccggggca tacatggatc
    15241 ccatccccca agccattccc
//
LOCUS       DBMDUH010000050        15219 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_2156_length_15219_cov_4.035917, whole genome shotgun sequence.
ACCESSION   DBMDUH010000050 DBMDUH010000000
VERSION     DBMDUH010000050.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 15219)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 15219)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15219
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2156_length_15219_cov_4.035917"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="ACZPCG_06005"
     CDS             <1..518
                     /locus_tag="ACZPCG_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HIR1284658.1"
                     /translation="QSVKAGEFRQELLDDDKGNGNHGFTLNVDWPSFSDGSYIIEATV
                     DGRTFSNTRTYTYGNSDTAKEEADSKASSSVRSLGIFKTTGYCPCKSCSGGWGRHTST
                     GAVATAGHTIAVDPSVVPYGSKIMINGVVYTAEDKGGGVRGHHIDIFYDTHSESKHHG
                     VQNTEVFLVAA"
     gene            complement(601..2886)
                     /locus_tag="ACZPCG_06010"
     CDS             complement(601..2886)
                     /locus_tag="ACZPCG_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HIR1284659.1"
                     /translation="MNGKNKMIAWIAGIIGVIILLIVVIVRIEPEKEGITRAEVSKAL
                     ALALADRKECEAEAKQREKSWFSEQEQDNWFVKYMDLLYERGYLNPELTPADLESAQK
                     EFTYQEAAYTAAKYSGRLKRQVGLKDKNRQKPFPADQWWELYREILKEADPDENVKEI
                     TAILYGTPSNIQGAPSWTAYTTEGEYGFQGLALDAWLDHEIRFMVRGNEIAAVTAVKN
                     GSVVYRNIWLSEVKDGVFKAYMGAASREFKAEGKLAEEAKKTEGRLAPNLADLYVEHG
                     RLSRVTVKKERIHGKILSVTEDTVEIEGYGTIPLDENFYVYKLFGEFSVLGAADILVG
                     YDLQEFVAADGKLCAALLERPFDARTIRVLLMDTGFKNLFHKKAELELNCDARLVCKN
                     DDGDSKESTLKKGEVLTLTAEDERLKHGRMTIIPEKEEGITICSLERAQGQPVYSGSL
                     EIKKEEEGLVLINELYLEDYLKKVVPSEMPASYEKEALKTQAVCARTYAYRQIQGNTY
                     GAYGAHVDDSTSFQVYNNTQTNERTDQAVDETYGQLLTFDGEPIDAFYYSTSCGHGAD
                     GSVWGNTGENLPYLRSMQIKNGARELTMEDNDTFDAYIRSRNITSYDAEYPMFRWQTE
                     ITAEQIAAKAPEAGTIQDLTVSARGLGGIASELTVKGDQDTVRIKGQSAIRTFLGNEE
                     NAIRRQDGSKMNAASLPSAFISVDKITEEDGSIFFRIYGGGYGHGVGMSQNGAQSMAK
                     SGKTYKDILDFFYHGAELMEN"
     gene            complement(2946..3122)
                     /gene="ssrS"
                     /locus_tag="ACZPCG_06015"
     ncRNA           complement(2946..3122)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACZPCG_06015"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(3197..3646)
                     /gene="ybaK"
                     /locus_tag="ACZPCG_06020"
     CDS             complement(3197..3646)
                     /gene="ybaK"
                     /locus_tag="ACZPCG_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009537776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HIR1284660.1"
                     /translation="MRLLEAAGISFETGTYEVDENDLSGSHAADLMGVDHDTMYKTLV
                     LKGEKKGYLVCCLPVDEELDLKKAAKAAGEKKVEMIHVKDMLSVTGYIRGGCSPIGMK
                     KKFPTYIEETAQLYDKITVSAGQRGVQVTLSPETLRGYTDAVFAPLI"
     gene            complement(3717..5414)
                     /locus_tag="ACZPCG_06025"
     CDS             complement(3717..5414)
                     /locus_tag="ACZPCG_06025"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR1284661.1"
                     /translation="MDIVKFEELQLDERIIRAITEMGFEEASPIQAQAIPVVLEGRDM
                     IGQAQTGTGKTAAFGLPLLQKVDPKVKKLQAIVLLPTRELAIQVAEELRRFAKFMHGV
                     KVLPIYGGQDIVRQIRALKDGTQIVVGTPGRVMDHMRRKTVKTDHVHTVVLDEADEML
                     NMGFLEDMETILSQLPEERQTLMFSATMPQAIAEIARKFQTDPVTVRVIKKELTVPKV
                     TQYYYEVKPKNKVEVMCRLLDLYDPKLSVVFCNTKRQVDELVQALQGRGYFAEGLHGD
                     LKQVQRDRVMESFRKGRTDILVATDVAARGIDVGNVEAVFNYDIPQDDEYYVHRIGRT
                     GRAGREGKAFSLVVGREVYKLRDIQRYCKTKIIPQAIPSLNDITEIKAEKILDQVQET
                     LNDSDLAKVVNILEKKLIEEDYTSLDVAAALLKMAMGDDNEDIIDNYLPARSLDDLDS
                     FGRGRGRERSRGGRGNRRGATDRAAVDYVINGAGEHMARLFINIGKAQRVTPGDILGA
                     VAGESGIPGRLVGSIDMYDGYTFVDVPAEYAENVLKAMSHAKIRGKNIHVEKANSRR"
     gene            complement(5556..6659)
                     /gene="ulaG"
                     /locus_tag="ACZPCG_06030"
     CDS             complement(5556..6659)
                     /gene="ulaG"
                     /locus_tag="ACZPCG_06030"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HIR1284662.1"
                     /translation="MSKVNEITRESWIMSTFPEWGTWLVEDIENEVVEPGNVAMWWLG
                     CTGIWFKTPGGANITIDLWCGNGKRTHGDGKMKPGHQMANMCGGRAMQPNLRNVPFVI
                     DPFAFKQVDAVLATHYHQDHMSAEWAAHVIQSGMTTTDENGKEIPVPFIGPQKSVDTW
                     IGWGVPAERCQVVHPGDVIKIKDIEIVCLDSFDRTCIVTTDKTGPDREELTGVCPTDM
                     DEKAVNYLVKTPGGNIYHSGDSHFSIYFAKHGKDHDIDVAFGSFGENPVGMQDKMTST
                     DILRMAENLKCKVVVPIHWDVWTNFQADCEEIRLLYDFKKDRNEYKFHPFFWQVGGKY
                     VYPQDKDKMYYHHRRGFEDCFEAPQNIPFRSCL"
     gene            complement(6700..8166)
                     /locus_tag="ACZPCG_06035"
     CDS             complement(6700..8166)
                     /locus_tag="ACZPCG_06035"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HIR1284663.1"
                     /translation="MNILVIDVGTSSMRGILFTQDGETLTQRQQLYQVTYGEKGWVWQ
                     DASDWENALYGIIREITAETEEKGWRVDAIAITSQRSSVIPVDREFKPLCPAVMWQDK
                     RTGDICDSLRDKNDKVFSLCGSRVNPVFSASKMTWIRRELPEVYSKTRTFMVIPDYLI
                     FCMTGEVCTDYTYGSRSLLMNIRKRQWDPELLELFEVDEEKLCRLIEPGSICGYTSGT
                     FRERTGCPEGVPVITAGGDQQCGAIGQGVVKEGILSVTAGTGGFLVAALNEVPSNLEQ
                     DVICNVSSVKGQYILESSVLTCCSAFDWFKREFYGNCSYDELNRDIASVPPGSHGCLC
                     LPYFQGRSTPDWNSQARGVFSNITLGTTRADMLRGLLEGICYEIGNGIEAMKKYVELS
                     EICVNGGLTNSEPFNQIQSDVYGTPIVRRGKSDATARGAFMVAAAEMGVYRSVEEAFR
                     MLKKNSEERIYVPSEAYTGLYREAREQMNQMYRQIYSR"
     gene            complement(8186..9043)
                     /locus_tag="ACZPCG_06040"
     CDS             complement(8186..9043)
                     /locus_tag="ACZPCG_06040"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009267365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1284664.1"
                     /translation="MSNIIEKYVKKKEYLVCVDSDGCAMDTMDVKHFRCFGPCMIREW
                     KLEQWKEETEKRWNDVNLYTMTRGINRFKALEAVLRWVDGHVCKIEGLEDLAKWTDTA
                     KELSPANLMAAIEAGGGDILKKAFDWSAAVNREIDALPESEKLPFEGVKETLAMIHEK
                     ADIVVVSSANAQAVAEEWQTHGLAGHTDLMLSQDAGSKQFCINELLKKGYQTDHVLMV
                     GDAPGDRSAARNNGVLYYPILVKKETYSWKRLQEEGMKRFLTGTFKGEYERQLEQEFE
                     DNLTPKTEQ"
     gene            complement(9081..10826)
                     /gene="uidA"
                     /locus_tag="ACZPCG_06045"
     CDS             complement(9081..10826)
                     /gene="uidA"
                     /locus_tag="ACZPCG_06045"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF007538.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HIR1284665.1"
                     /translation="MLYPKMTESRMVFDLNGVWDFCLMNGEAKGEIMPMPVPSSYNDI
                     YTGRDFADHVGNLYYRRTFTVSSRMLEKRLFLRFGSVTHKAEVWLNGTCLGKHSGGFL
                     PFSFEITEAARAGENELEVIVNNIVDETTLPAGRMVHQKFPGLPEEIHNLPNFDFYNY
                     SGIMRPVCLYTAPESYIEDISIYGKMDGSFYWDVKANGEGTVSVRLLDAAGNEVAAGE
                     GFKGTGRIDQVQLWEPGHPVLYSLEVTLTGSDGEKDTYTEVFGFREVSIRDCRIHLNG
                     KPVYLKGFGKHEDSPVHGRGFDMAYNVKDIGLLKWIGANSFRTSHYPYCEEMLQLCDR
                     EGILVIDEAPAVGLNAGFTATGLLGGNPNGTWKLFKTAEHHRQVLRDMVQRDKNHPCV
                     IIWSVANEPASQEDGAKEYFEPLLNLVRELDEQKRPATLVTYEGSNPVSCKVAEICDL
                     LIINRYRGWYDTEGNLRGAAALLKDELEGFHKRCPDKPIMLGEYGADTIAGFHDINAR
                     IFSEEYQVDFLRAYGEVFDSLPYITGEHVWNFADFATAENIKRVGGNKKGVFTRDRSP
                     KMAAHFLKERWEGIS"
     gene            complement(10901..12280)
                     /locus_tag="ACZPCG_06050"
     CDS             complement(10901..12280)
                     /locus_tag="ACZPCG_06050"
                     /inference="COORDINATES: protein motif:HMM:NF013697.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1284666.1"
                     /translation="MKKSKRFFAAMMAAAMTAGMLTGCGGGAKKESAAPAESAQGGST
                     EAAKEGEQVNLRFVSWQTANKELDKKVAEAYTKEHPNVTITYDYYGDMTAAEYYKKVD
                     MMVMGGEEMDILMASAFPEHAQRAASGAYLPLEEFFEKEGVKPEDAYAIVQKVDGKMY
                     GIPGDLKSWFVLINKNYLDEAGLPVPELDWTWADYRDYANKMTQGEGASKRYGSYFHS
                     WDHYNYMGMWSNYPDNPMFNADMTAVNFDHPMFKEWLQFRYDMENEDKCQVPYADVKS
                     MNMNYRDKFFNGQIAMLPIGSFMIPELDDQDKYPHEFVTTFAPIPAWEDAEPGTTYTE
                     SHFYSISKTSKHPQEAYDYIRFYTTEGMRIRGISVSAEKGIDKMEFVNMQIEDPKYVD
                     VEALKNVINNPAWKDNLYANVPSYNKELAAMMTDESAKFLLGTESLDNVIDSMMKNGT
                     QMMKDKAGK"
     gene            complement(12320..13150)
                     /locus_tag="ACZPCG_06055"
     CDS             complement(12320..13150)
                     /locus_tag="ACZPCG_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012198559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1284667.1"
                     /translation="MKKLNITKVILTAIFWAVGISMVLPLIWMMSTACKVEADVFNFP
                     IQWIPPRWNLVENMKEVWGGTYNFGLYYLNTIKVTLGATFLQVFVSALGAYGFSKVKF
                     KGRDKIFLAYLATMMIPPQVTIVSQFIIMREIGLYNSHAGLILMLAFSVYGVFLLRQA
                     MLAIPESLSESAMIDGAGHVTIFFRIIMPMIKPSLATLATLKFVWTWNDYQAPLVFLN
                     NRALYTIQLGMKQFASESGSYYSLIMAAAVSAILPLIIVFLFCQRFVVDGIATGAVKG
                     "
     gene            complement(13165..14064)
                     /locus_tag="ACZPCG_06060"
     CDS             complement(13165..14064)
                     /locus_tag="ACZPCG_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1284668.1"
                     /translation="MNTKGSKKARKDFFTGMGFILPSLLGFLIFTFIPVVISLCLSFT
                     SWNFMEGIEGIKFNGLANYIRLFSDEWFLNSYKNNIIFTAVTVPVLIALGLVMATIIN
                     KYIYGGGVVRTMIFIPYIASVVAVCTVWMVLLQPSYGPVNEFFRSIGIANPPGWLADF
                     KWSLPSIMIIYVWQQVGYYSIVFLAGLKGLPEDVYEAAKVDGASSIRQFFSLTVPLIS
                     PTTFFLTIMGIIGSFKVFDQISVLTQGGPGSSSSVMAYYVYRTAFDYFEMGYANTLAW
                     ALFVLVFIVTLVQWKMQAKFSNE"
     gene            complement(14086..>15219)
                     /locus_tag="ACZPCG_06065"
     CDS             complement(14086..>15219)
                     /locus_tag="ACZPCG_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HIR1284669.1"
                     /translation="MGVNTMECDEWDSTFGHVYKEMIDSGIQSIMAGHIALPEYSRKL
                     RPGIKDEDIMPATLAPELITDLLRGKLGFNGLVLTDASHMAGMTAAMPRSQQVPGAIA
                     AGCDMFLFFNDIEEDFGYMMDGYRNGIITEERLTDALRRILGLKASLKLHEKKKKGTL
                     IPEKEGLSVVGCAEHLAMAAEAAKKSITLVKDTQHNLPVTPKTHKRAYAFVISSPPIS
                     RKNQHDPVKQLVKEEMERFGYETTMHESYYDLVLDDGITKETELRSVIIGKVEEFKKK
                     YDVVFVFINMKGYAQANNVRLEWSIGHSTEIPWYVKELPTIFISLNYTNHLLDVPMAK
                     TFINAYAPTREVIRQTIEKAAGEAAFEGTCNDNVFCGRWDTRL"
BASE COUNT         3880 a         4076 c         2900 g         4363 t
ORIGIN      
        1 atcaatccgt aaaggccgga gaattccgcc aggagctgct cgatgacgat aaaggcaacg
       61 gaaatcatgg ctttactctg aatgtggact ggccctcctt cagcgacggc tcctatatca
      121 tcgaggctac ggtagacgga cgcacttttt ccaacacaag aacctacacc tatggaaatt
      181 cggatacggc aaaggaagaa gccgattcta aagcctcctc ttctgtccgc tctctgggaa
      241 tctttaagac cactggctac tgcccctgca aaagctgttc cggcggctgg ggccgtcata
      301 ccagtacagg cgccgtggct actgccgggc ataccattgc agtcgaccct tctgtggtac
      361 cctacggctc caaaatcatg atcaacggcg tcgtatatac cgcagaagac aaaggtggcg
      421 gagtccgggg ccaccacatt gatattttct acgatacaca ttccgaatcc aagcatcacg
      481 gcgttcagaa tacagaagtt tttctggtag cagcataacc catatcagac cactcactca
      541 caaaaacagc aggcttatct gagaaatact cccagatcag cctgctgttt tttctgttat
      601 ttaattttcc ataagctctg ctccgtgata aaagaagtcc agaatatcct tataagtttt
      661 tcctgatttt gccatagact gagcgccgtt ctggctcatc cccactccat gtccgtagcc
      721 tcctccatag atgcggaaaa atatgctccc gtcctcctct gttattttat caacagagat
      781 aaatgcactt ggcagagacg ctgcgttcat cttagaacca tcctgtctgc ggatggcatt
      841 ttcttcattt ccaagaaagg tacgaatggc actctgtccc ttgatccgaa ccgtatcctg
      901 atctcccttt acagtgagct cagaagcaat gcctcctaat cctcttgcgg acactgtcaa
      961 atcctggatc gttcctgcct caggcgcttt ggctgcgatc tgttctgccg tgatctccgt
     1021 ctgccagcgg aacattggat actctgcatc ataggatgta atatttctgg aacggatata
     1081 cgcatcaaag gtatcgttat cctccatcgt aagctccctg gctccatttt taatctgcat
     1141 ggatctgaga taaggaagat tctctcctgt attcccccat acgcttccat ccgccccatg
     1201 accgcaggag gtggaatagt aaaaggcgtc aatgggttct ccatcaaagg taagcagctg
     1261 tccatatgtt tcatccaccg cctgatcggt acgttcattg gtctgggtat tattgtaaac
     1321 ctgaaaactg gtgctgtcat ctacgtgagc accgtacgct ccgtatgtat ttccctgaat
     1381 ctgacggtaa gcataggttc tggcacagac tgcctgggtc tttaaggctt ccttttcata
     1441 ggaagcaggc atttcgctgg gaactacctt tttaagataa tcctccagat acagctcatt
     1501 gatcaaaacc agaccttcct cttctttttt aatttccagc gaaccgctgt atacgggctg
     1561 tccctgtgcc cgctccaggg agcagatcgt aattccctct tccttttccg ggataatggt
     1621 catacggccg tgtttcagcc gttcatcctc cgccgtgagg gtaagcacct cccccttttt
     1681 cagggtactc tccttgctgt ctccgtcatc atttttgcag accagtctgg cgtcacagtt
     1741 taactcaagc tctgcctttt tatggaaaag atttttaaag cccgtatcca tcaaaaggac
     1801 tcgaatggtc ctggcgtcaa aaggcctctc taaaagggct gcgcaaagct ttccatctgc
     1861 tgccacaaac tcctgaagat cataacctac cagaatgtct gcggctccca gaacactaaa
     1921 ttccccgaac agcttatata cgtaaaaatt ttcatccaga ggaatggtcc cgtatccttc
     1981 aatctctacc gtatcttctg taaccgaaag gatttttccg tgaatccgtt cctttttcac
     2041 cgttacccgg ctgagacggc catgctccac atagagatct gccagattgg gagccagacg
     2101 gccttctgtc tttttcgctt cctccgccag ctttccttct gccttaaact ctctggacgc
     2161 cgctcccata taagccttaa acacgccgtc ctttacctca gaaagccata tattgcggta
     2221 gaccacactg ccgtttttta ccgccgtcac agcggcaatc tcatttcccc gaaccataaa
     2281 ccggatctca tggtccagcc aggcatccag ggccagtccc tgaaagccat actccccctc
     2341 tgtggtgtat gcagtccagc ttggcgctcc ctgtatatta gatggagttc catataaaat
     2401 ggcagtgatt tcttttacat tttcatccgg gtctgcctct tttaaaatct ctctgtaaag
     2461 ctcccaccac tgatctgccg gaaatggctt ctgtctgttt ttatccttta aacccacctg
     2521 ccgctttaaa cggcctgaat attttgccgc tgtataagct gcctcctgat aggtaaactc
     2581 cttttgagcg ctttccagat ctgccggggt cagctccggg tttaaatatc ctctctcata
     2641 gagcagatcc atatatttca caaaccagtt atcctgttcc tgctccgaaa accaggattt
     2701 ttcccgctgt tttgcttcgg cctcacactc tttccggtct gcaagcgcca aagccagggc
     2761 tttggaaacc tctgcccgtg tgatgccttc cttttccggt tctatccgta cgatcaccac
     2821 aatcagcagg ataatgacgc caatgatacc ggcaatccag gcaatcatct tatttttacc
     2881 attcatccca ttattctcct gatataaaat aaagcagcga gaataccccc gctgctttta
     2941 tcatctgtga ccccaaggtg ccggctctta catattgacg cctagcaatg ctaggtgggc
     3001 aacctcactt aagcttctgc cttccactcg tacggaggcg tgacatccgc ttaaaaatat
     3061 tggaatccct tttgtcaaat gtaggttcaa aattggtaag aaatcgaaca ccccaggaat
     3121 cacaaatccg tctcatttat catggcatta ttatacccca aagcgctgtg tttttcaagc
     3181 tgatttttca aagttttcag atcaggggcg caaaaacagc gtccgtatat ccccttaaag
     3241 tttccggaga taatgtaacc tgaacacctc tctgaccggc gctgactgta attttatcgt
     3301 aaagctgggc tgtttcctct atataagtag gaaatttctt tttcatgcca atgggtgaac
     3361 agccgccccg gatatagccg gtcacggaaa gcatatcctt tacatggatc atttccacct
     3421 tcttctctcc ggctgccttt gccgcttttt taagatccag ctcctcatct acaggcaggc
     3481 agcacaccag atatcctttt ttctcgcctt ttaacaccag tgttttatac atcgtatcat
     3541 ggtccacacc catgagatcg gctgcatggc tgccggaaag atcattttca tctacctcat
     3601 aggttcccgt ttcaaatgaa atgcctgctg cctctaaaag acgcatcaca tttgtcttta
     3661 ctgtctcttc tgcctttttt gccttcacgg ctgatccctt ctctctttac gttctgttac
     3721 cgtctggaat ttgccttttc cacatggata ttttttcccc ggatctttgc atggctcatg
     3781 gcctttaaaa cattctccgc atattctgcc ggaacatcca caaaggtgta gccgtcatac
     3841 atatcaatgc ttcccaccag acggccggga atgccggatt cgcctgccac ggctcccagg
     3901 atatctcccg gagttaccct ctgggccttt ccaatattga taaacagacg ggccatatgc
     3961 tccccggcgc cattgatcac ataatctact gcagcgcggt cggtagcgcc tctgcggttt
     4021 cctctgccac cccggctgcg ttctcttcca cgccctctgc cgaagctgtc cagatcatct
     4081 aaggatctgg caggaagata attgtcaatg atatcctcat tatcatcgcc catggccatt
     4141 ttaagaaggg ctgctgccac atccagggac gtatagtcct cctcaatgag ctttttctcc
     4201 aggatattta ccacttttgc cagatcgctg tcatttaaag tctcctgaac ctgatctaaa
     4261 atcttctccg ccttgatttc cgtaatatca tttaaagacg ggatcgcctg agggatgatc
     4321 tttgtcttgc agtagcgctg gatatctctg agcttgtaaa cctctctgcc cacaaccagg
     4381 ctgaaggcct ttccttctct tcccgctctt cctgtacggc cgatgcggtg tacgtaatac
     4441 tcgtcatcct gagggatatc gtaattaaat actgcctcta catttcctac gtcaatacct
     4501 ctggccgcca catcggttgc caccagaata tcggtgcgtc ccttgcggaa gctctccatg
     4561 acacggtctc tctgcacctg ttttaaatct ccatgaaggc cctctgcaaa gtatcctctt
     4621 ccctgaaggg cctgaaccag ctcatccacc tggcgcttgg tattacagaa taccacagaa
     4681 agcttcggat cgtaaagatc cagaagacgg cacataacct ctaccttatt tttcggcttt
     4741 acttcataat aatactgggt caccttggga acggtcagtt cctttttgat cacacgcacg
     4801 gtaacaggat cagtctggaa ttttctggca atttctgcaa tcgcctgggg catggttgct
     4861 gaaaacatca gcgtctgacg ctcttcggga agctggctta agattgtctc catatcctct
     4921 aaaaagccca tatttaacat ctcgtccgcc tcatccagta caacggtatg cacatggtct
     4981 gttttcacgg ttttgcggcg catatgatcc atcacgcggc ccggtgttcc cactacgatc
     5041 tgggtaccgt cttttaaagc ccggatctgg cgcacaatgt cctgtcctcc ataaataggc
     5101 agaaccttaa cgccgtgcat aaacttggca aaacggcgca gctcctctgc cacctggatt
     5161 gcaagctctc tggtaggcaa aagcacaatt gcctgaagtt tcttcacctt tggatcaacc
     5221 ttctgaagaa gggggaggcc aaaagctgcc gtctttcccg tacccgtctg agcctggccg
     5281 atcatatctc tgccctctaa cacaaccggg atcgcctgag cctggatggg agaagcctcc
     5341 tcaaagccca tctccgtaat agcgcggatg atccgctcgt ctaactgtaa ctcttcaaat
     5401 tttacaatat ccataaactg ctgttttcct ctttcattta tcagtgtata tcgcaacaaa
     5461 acgagaggtt gccagccacg ctcggctttt catcctctcg cttcgtacag tgaattacta
     5521 tatcagatta tgtttgctgt gtcaatgaag aagtcttaca gacaagagcg gaacgggata
     5581 ttctgaggtg cttcaaagca atcctcaaag ccacggcgat ggtgataata cattttatcc
     5641 ttatcctgcg gatatacata ttttccgcct acctgccaga agaacgggtg gaatttatac
     5701 tcatttctgt ctttcttaaa atcgtaaaga agacggatct cctcacagtc agcctggaag
     5761 tttgtccaga catcccagtg aataggaact actaccttac acttaagatt ctctgccata
     5821 cgcagaatat ctgttgaagt catcttatcc tgcatgccca ctggattttc accaaaggat
     5881 ccaaaagcca catcaatatc atgatccttt ccatgctttg caaaataaat ggagaaatgt
     5941 gaatcacctg aatgatatat atttcctccg ggagttttta ccagatagtt aactgccttc
     6001 tcatccatat cagtaggaca tactcctgtc agttcttctc tgtccggtcc agtcttatca
     6061 gttgttacaa tacaggtacg atcaaaggaa tccagacata caatttcaat atccttaatc
     6121 ttgattacat ctcccggatg tactacctga caccgttctg caggtacgcc ccagccaatc
     6181 caggtatcaa ctgatttctg agggccaata aacggaaccg gaatttcttt tccgttttca
     6241 tctgtcgttg tcataccgct ctgaatcaca tgagcagccc attctgccga catatgatcc
     6301 tgatgatagt gtgttgctaa aactgcatca acctgcttga atgcaaatgg atcaattaca
     6361 aaaggaacgt ttcttaaatt tggctgcata gcacggccgc cgcacatatt ggccatctga
     6421 tgtcccggct tcatcttacc gtctccatgg gttctcttac cgtttccgca ccataaatca
     6481 attgtgatat tagctcctcc cggtgtttta aaccagattc cggtacagcc cagccaccac
     6541 atagctacat ttccgggctc gacaacctca ttttcaatat cctctaccag ccaggttccc
     6601 cactcaggga aagtgctcat aatccagctt tctcttgtga tttcattcac tttactcatt
     6661 gcgcatcgtc tcctttttat attgggtgtg tcatccgttt tatctgctgt aaatctgtct
     6721 gtacatctga ttcatctgct ctctggcttc cctgtacagc ccggtatatg cttctgacgg
     6781 aacatatatc cgttcttcac tgtttttctt aagcatcctg aatgcctctt ccacagatct
     6841 gtatactccc atttctgctg ccgctaccat aaatgcacct cttgccgttg catctgattt
     6901 tccacggcgc acgattggtg ttccatatac atcgctctgg atctgattaa atggttcgct
     6961 gtttgtaagt cctccgttga cacagatttc cgacagttcc acatattttt tcatggcttc
     7021 aattccattg ccgatttcat agcagattcc ttccagaagg cctcgaagca tatcagcacg
     7081 tgtagttccc agggttatgt ttgaaaatac acctctggcc tgactgttcc agtccggagt
     7141 agaacgtccc tggaaatatg gaagacacag acagccatgg gatcccggcg gaacagatgc
     7201 tatatcccgg ttcagctcgt catagctgca gtttccgtaa aattctcttt taaaccagtc
     7261 aaatgcagaa cagcatgtca aaacactgga ttccagaata tactgaccct ttacagatga
     7321 tacgttgcag attacatcct gctcaagatt ggatggtacc tcatttaatg ctgcaacaag
     7381 aaatcctcct gttcctgctg taactgataa aattccttct tttactactc cctgtccgat
     7441 agccccgcac tgctggtctc cacctgcagt gatcacagga accccttctg ggcatccggt
     7501 gcgttcccgg aatgttccgg atgtataacc acagatactt cccggctcta tgaggcggca
     7561 cagcttttcc tcgtctactt caaacagttc cagaagttcc ggatcccatt gacgcttccg
     7621 tatattcata agcagagacc ggcttccata cgtataatct gtacatactt cacctgtcat
     7681 acagaatatg agataatccg gaattaccat aaatgtcctt gttttgctgt atacctctgg
     7741 cagttcccgg cggatccagg tcatcttgct ggcagaaaat accggattca ccctggatcc
     7801 gcaaagcgaa aataccttgt cgtttttatc tctgagactg tcgcagatat caccggttct
     7861 cttatcctgc cacataacag ccggacacag aggcttaaat tcccggtcta ccggaataac
     7921 agatgaacgc tgggatgtaa ttgcaatggc atccaccctc catccctttt cctctgtctc
     7981 cgcagtgatt tccctgataa taccatataa ggcattttcc cagtcagaag catcctgcca
     8041 gacccagccc ttttccccgt aagtaacctg atagagctgc tgcctttgtg ttaatgtttc
     8101 tccatcctgt gtaaacagga ttcccctcat actggaggtt ccaacatcta ttacaagaat
     8161 attcatcctg ttacggcttc ctttcttact gctcggtttt cggtgtcagg ttatcctcaa
     8221 attcctgctc cagctgccgt tcatattctc ccttaaaagt acctgtcagg aaccgtttca
     8281 ttccttcttc ctgaaggcgc ttccatgaat acgtctcttt tttaaccagg atcgggtaat
     8341 agaggactcc gttatttctg gctgccgaac ggtctcccgg cgcatcccct accatcagaa
     8401 cgtgatctgt ctgatagccc ttttttaaca gttcattgat acaaaactgt ttgcttcctg
     8461 catcctgaga aagcatcagg tcagtatgtc cagccagccc atgggtctgc cactcctctg
     8521 ccacagcctg tgcattagcg gaagagacaa caacaatatc tgctttttca tgaatcatag
     8581 ccagcgtttc ttttactccc tcaaagggca gtttttcact ttccggcagg gcatcaatct
     8641 cccggtttac agcagccgac cagtcaaagg ccttttttaa gatatctccg cctcctgcct
     8701 caatggctgc catcagatta gccggtgaaa gttcctttgc agtgtctgtc cactttgcca
     8761 gatcctccag cccctcaatt ttacatacat gcccgtccac ccagcgcagt accgcctcca
     8821 gcgccttaaa gcggttaatt cctctggtca tcgtatacag attaacgtca ttccaacgct
     8881 tctctgtttc ctctttccac tgctcaagct tccactcccg gatcatacat ggaccaaagc
     8941 agcgaaaatg tttcacatcc attgtatcca tagcacagcc gtcagaatcc acacaaacca
     9001 gatattcttt ttttttcaca tatttttcta ttatgtttga catactcacc tctatttgac
     9061 agttcttatc ccaactgtcc ttatgatatt ccttcccatc tttccttcag gaaatgggct
     9121 gccattttag gactacggtc gcgcgtaaac actccctttt tattaccccc aacgcgtttg
     9181 atattctctg ctgttgcaaa atctgcaaag ttccatacat gttcacctgt tatatatgga
     9241 agactgtcaa aaacctcacc atatgcccgc aggaaatcaa cctgatactc ttcgctgaat
     9301 atacgggcat ttatatcatg gaaaccggca atggtatctg ctccgtattc tcccagcata
     9361 ataggtttat cgggacaccg cttatgaaag ccctcaagct catcctttaa aagagctgct
     9421 gctcctctga gatttccctc cgtatcatac cagccgcgat agcggttgat gatcaaaaga
     9481 tcacagattt cagccacctt gcagctgaca gggttagaac cttcatatgt aaccagtgta
     9541 gccggacgtt tctgttcatc caattcccga acaagattta acaatggttc gaaatattcc
     9601 tttgctccgt cctcctggct ggcaggctca tttgcaacac tccagataat aacacaggga
     9661 tgatttttat ctctctgaac catatcacgc agaacctgac ggtgatgctc agccgtctta
     9721 aaaagcttcc atgttccgtt gggattgcct cccagaagtc ctgtagccgt gaagccggcg
     9781 ttcagtccta ccgcaggagc ctcatcaatg actaaaattc cttctctgtc acaaagctga
     9841 agcatctctt cacaatatgg ataatgactg gtacggaaag agttggcacc gatccatttt
     9901 aacaaaccaa tatcctttac attgtatgcc atatcaaagc cgcgtccgtg aacaggtgaa
     9961 tcttcatgtt ttccaaatcc ctttaaatat accggcttac cgtttaaatg aatccggcag
    10021 tcacgaatcg atacctccct gaatccgaac acttcagtgt atgtatcctt ttctccgtca
    10081 cttcctgtaa gagtaacctc cagactgtat aaaaccggat gtccgggttc ccacagctgt
    10141 acctgatcaa tccgtcctgt tcctttaaag ccctcgcccg ctgcaacttc atttcccgca
    10201 gcatccaaaa gacgtacaga tactgttccc tctccatttg cctttacatc ccagtaaaag
    10261 cttccgtcca tttttccgta aatactgata tcttcaatgt atgattcagg agcagtataa
    10321 aggcagacag gacgcataat tccggaataa ttgtaaaaat caaaattagg aaggttatga
    10381 atctcttccg gcagccccgg aaacttctga tgcaccatac ggcctgccgg cagcgttgtt
    10441 tcatctacta tattatttac aattacttcc agttcgtttt cccctgctct ggccgcctcc
    10501 gtaatttcaa aggaaaatgg aagaaaaccg ccagagtgtt ttccaagaca agttccattc
    10561 agccatacct ctgccttatg agttacactt ccaaaccgaa gaaacagacg tttttccagc
    10621 atccgggaac ttactgtgaa cgttcttctg taatacagat ttcctacatg atctgcaaaa
    10681 tcgcggccag tgtagatatc attataagaa gaaggtaccg gcataggcat aatttccccc
    10741 tttgcctccc cattcataag acaaaaatcc catactccat ttaaatcaaa aaccattctg
    10801 ctttctgtca ttttcggata cagcataaat attcctctct ttcattagaa tagcaaaggg
    10861 gaaggggcat accacccctc ccccgattca ttctgcttaa ttacttgcct gccttatcct
    10921 tcatcatctg agtaccattt ttcatcatgg aatcaattac gttatcaagg ctttctgttc
    10981 ctaataagaa tttagcactt tcatctgtca tcatagctgc taactcctta ttgtaagaag
    11041 gaacatttgc atacagatta tccttccaag ccggattgtt gattacgttc tttaatgctt
    11101 ctacatctac atacttagga tcttcgatct gcatattaac gaattccatc ttatcgattc
    11161 ccttttctgc agaaacactg atgccacgga tacgcatacc ctctgtggta tagaaacgga
    11221 tgtagtcata agcttcctga ggatgcttgg atgtcttgct gatgctgtag aaatgggact
    11281 ctgtataggt agttcctggc tctgcatctt cccatgcagg aattggtgca aaggtagtta
    11341 caaactcgtg aggatactta tcctggtcat ccagctcagg aatcatgaag cttccgattg
    11401 gcagcattgc gatctgtccg ttgaagaact tatcacggta gttcatgttc atagacttaa
    11461 cgtctgcata aggaacctgg cacttatctt cattttccat atcgtaacgg aactgcagcc
    11521 actctttaaa cattggatga tcaaagttta cagccgtcat atcagcattg aacattgggt
    11581 tgtccggata gttggaccac attcccatgt agttataatg atcccaggaa tggaaatagg
    11641 aaccatagcg cttacttgcg ccttcaccct gtgtcatctt gttggcatag tcacgataat
    11701 ctgcccatgt ccagtcaagc tccggaacag gaagaccagc ctcatccaga tagttcttat
    11761 taatcagtac aaaccatgac ttcagatctc caggaattcc atacatcttt ccgtcaacct
    11821 tctgtacgat agcatatgcg tcctcaggtt ttacaccttc cttctcaaag aactcttcca
    11881 gaggcagata tgcgccggat gctgctctct gagcatgctc cgggaatgca gatgccatca
    11941 ggatatccat ttcctcgccg cccattacca tcatatctac ctttttgtag tactctgctg
    12001 cagtcatatc accatagtag tcatatgtaa tggttacatt aggatgctct tttgtgtaag
    12061 cctctgctac cttcttatcc agttccttgt tagctgtctg ccaggaaaca aagcgcagat
    12121 taacctgctc accttccttg gcagcctctg tgctgccgcc ctgagcactc tctgcaggag
    12181 ctgctgattc ctttttagct ccgccgccgc atcctgtcag cattcctgct gtcatggctg
    12241 ctgccatcat ggctgcaaaa aaccgtttgc tttttttcat tttaaaaacc ctccttaaat
    12301 tatatatata atacatttgt taacccttta ctgctcctgt agcaattccg tctactacaa
    12361 accgctgaca gaacaggaac acaatgatca atggcaaaat tgcggaaact gccgcagcca
    12421 tgatcagaga ataataactt cctgattcag aagcaaactg cttcatacca agctggatcg
    12481 tataaagtgc acggttgttt aagaatacca agggagcctg gtaatcattc catgtccata
    12541 cgaatttcag ggttgccagt gtagccagag atggcttgat cataggcata ataatcctga
    12601 aaaagatagt tacatgacct gcaccatcaa tcatggctga ctcagacagg gattcaggaa
    12661 tagcaagcat tgcctgacga agcaggaaca cgccgtatac actgaacgcc agcataagga
    12721 tcagacctgc atgagaattg taaagtccga tctcacgcat gataatgaac tgtgaaacga
    12781 tggtaacctg aggcggaatc atcatggttg caaggtaagc caggaagatt ttatcacgtc
    12841 ccttaaattt caccttggaa aatccataag cgcccagagc agaaacaaat acctgaagaa
    12901 aggttgctcc cagtgttacc tttatggtat ttaagtaata cagtccaaag ttataggtac
    12961 ctccccaaac ttctttcata ttttccacca gattccaacg gggaggaatc cactgaatag
    13021 ggaagttaaa tacatccgcc tctaccttac aggcagtaga catcatccag atcagaggca
    13081 gcaccatgga aatacccact gcccagaaaa tagctgtcag gattactttt gtaatattga
    13141 gttttttcac ctgtatgccc ctccttactc atttgaaaac tttgcctgca tcttccactg
    13201 aaccagtgta acaatgaata ccagtacgaa aagcgcccat gccagggtat tggcataacc
    13261 catttcaaag taatcgaaag cagtgcggta tacatagtaa gccataacgg agctggagct
    13321 tccaggaccg ccctgggtca gtacactgat ctgatcgaat accttgaagg aaccgatgat
    13381 acccataatt gtaaggaaga aggttgtagg ggaaatcagc ggcactgtca atgaaaagaa
    13441 ctgacggatc gaagacgcac catctacctt tgcagcttca tacacatcct ctggaagtcc
    13501 ctttaaacct gccaggaata cgatagaata gtatccaacc tgctgccata catatatgat
    13561 catgatcgat ggaagagacc acttaaagtc agccagccag cctggaggat ttgcgattcc
    13621 gatggaacgg aaaaattcat tcactggtcc gtaactgggc tgtaaaagaa ccatccacac
    13681 cgtacataca gcaacaacac tggcgatata aggaatgaag atcatggtac gcactacacc
    13741 gccgccataa atgtacttgt tgataatagt agccattaca agtcccagtg cgatcagaac
    13801 aggcactgtt actgcagtaa atataatgtt gttcttgtag gaattaagga accactcatc
    13861 tgagaagaga cggatatagt ttgcaagacc attaaatttg attccctcta taccttccat
    13921 aaagttccag cttgtaaagc tcaggcacag ggagattact acaggaataa aggtaaaaat
    13981 caaaaatccc aaaagactgg gaagaataaa tcccatacct gtaaaaaagt ccttgcgcgc
    14041 tttctttgat ccttttgtat tcataggtta attccccctt tctctctaca gcctagtatc
    14101 ccagcggccg cagaatacgt tatcattgca ggttccttca aacgccgcct caccagctgc
    14161 cttttcaatg gtctgacgaa ttacttctct ggtaggggca taagcattaa taaaggtttt
    14221 agccataggc acatccaaca aatggttggt ataatttaaa gaaataaata tggtaggaag
    14281 ttcttttaca taccacggaa tctccgtaga atgaccgatg gaccactcca gacgcacatt
    14341 attggcctga gcatatccct tcatattgat aaatacaaat accacatcgt atttcttttt
    14401 aaactcttct acctttccga ttatcacgga acgcagctca gtttcttttg taatgccatc
    14461 atccaatact agatcataat agctttcatg catggtggtt tcataaccga accgctccat
    14521 ctcctccttt accagctgct ttacaggatc atgctggttt ttacgtgaaa taggaggact
    14581 gctgattaca aaagcatatg ctctcttatg agtctttggt gtaacaggaa gattgtgctg
    14641 agtatctttt acaagagtga tactcttttt agctgcttct gctgccatag caagatgttc
    14701 tgcacatccc acaacagaaa gcccctcctt ttcaggtatc agcgtaccct tcttcttctt
    14761 ttcgtgaagc tttaaagaag cctttaatcc aagaatacgg cgcagcgcat ctgtaaggcg
    14821 ttcttctgtg atgattccat ttctataacc atccatcatg tagccaaaat cttcttcgat
    14881 atcattaaag aagaggaaca tatcacagcc tgctgcaatt gctccgggca cctgctgact
    14941 tcttggcata gcagctgtca ttccagccat atgggaggca tccgtaagaa caagtccatt
    15001 aaatccaagc ttacctctca gaagatccgt gatcagttcc ggcgccagtg ttgccggcat
    15061 aatatcctca tcctttatac ccggacgaag ctttctggag tactccggaa gtgcaatgtg
    15121 gcccgccata atgctctgaa ttccgctgtc gatcatttcc ttataaacat gaccgaaagt
    15181 agaatcccac tcatcacact ccattgtatt tacacccat
//
LOCUS       DBMDUH010000051        15044 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_2181_length_15044_cov_8.237569, whole genome shotgun sequence.
ACCESSION   DBMDUH010000051 DBMDUH010000000
VERSION     DBMDUH010000051.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 15044)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 15044)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15044
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2181_length_15044_cov_8.237569"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="ACZPCG_06070"
     CDS             complement(<1..228)
                     /locus_tag="ACZPCG_06070"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HIR1284670.1"
                     /translation="MKHWTWQTVMGLLMLGAVALLSWKAGTFVASGTLAVSSGKGGAG
                     SLRPAVVIDAGHGGRDPGKVGIEGQLEKDINL"
     gene            complement(325..1818)
                     /locus_tag="ACZPCG_06075"
     CDS             complement(325..1818)
                     /locus_tag="ACZPCG_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR1284671.1"
                     /translation="MKGKRWIAVALVCLAGVGIGGCSIFTGAPGDVREESETMGEDGL
                     VVPSASSQETEESSQTKPTKTEPESIPPFPDAERETKAEPMEDGERIVLMTDVHYLAR
                     SLTDRGSSFQNEIEHGDGKLTHYVWEIVDAAFDEIELLTPDVLIVSGDLTLNGEVKSH
                     EEFAALLDEVERRGISVLVIPGNHDINSRWASSYIGKNREPVQTIDAGGFASIYAQFG
                     YNEASERDPYSLSYTYDLSPSMRLLMLDTCQYSPVSKIGGMIGTETYEWIDRQLEKAR
                     EDGVILLPVGHHNLLDQSRVYTEDCTIEHSEELIERLEAENIPLFLSGHLHVQHFMRH
                     EDGRGIYEIVTSSLTTPPCQYGVLEYRDDESFSYYTRQVDMEKWARRHQSTDENLINF
                     NTYSKTALKNIFYNQAYDAMKNSRQEEKGDLFVKLTEEQKKAMAEVYSRMSTAWYAGK
                     GYEAVKGAVEDPGFKMWQEFCYPAVLYEYLEYMTADSVRDYNKLQSE"
     gene            complement(1861..3546)
                     /locus_tag="ACZPCG_06080"
     CDS             complement(1861..3546)
                     /locus_tag="ACZPCG_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HIR1284672.1"
                     /translation="MRSRRRKRRSFGPLLAVVFLLLLIAGAAAGFVIGRRYMPGKEMA
                     DKAELFHIKGSQVAILLNNELQEEKGIYEDGQVYLPISWVNEYVNERFYWDETEKLLV
                     YALPEEIVYADESDMGEQGPLLKVKEGEAYLSLGLIMNYSDIRQQSFDTSQIKRVFID
                     TVWGTVKTAQVRKKSILRVKGGIKSDIITELSEKSTVQVLESMDKWSKVRTEDGYIGY
                     VQNRRLEKEQEITPQGRFEAPVYTSISMDEKVRLGFHQVTRKEANSTLKEYAQTAEGM
                     NVIVPTWFNVIGNDGTYTSLASRDYVEQAHDMGLKVWAMVENVSTKESVKELDTKKLM
                     SVTSNRRKLIENLMKEADTYGFDGFNLDFESLKAEAGPHYVQFIREMSVACRKKGLVL
                     SVDNYVPSAYTAFYNRREQGIVADYVIVMGYDEHYAGGEAGPVASLPYVEKGIADTLK
                     EVPKEKVINSVPFYTRIWTEKDGKTTSKAYGIRDAKNWIKENNIELEWLDDLGVYYGE
                     SSNENGVQSVWMEEEKSLGLKVDLINEYDLAGAACWKLGFEDRSIWEIVSQVK"
     gene            complement(3770..4027)
                     /locus_tag="ACZPCG_06085"
     CDS             complement(3770..4027)
                     /locus_tag="ACZPCG_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284673.1"
                     /translation="MYGKESGEVRTQQMNYLDGLRRFRDKGVSVLVDGMECLEEEWSR
                     IFETGEDGGFYMGDFICSEQGSLKEIRFDRVYLTDSGQTRR"
     gene            complement(4161..4757)
                     /locus_tag="ACZPCG_06090"
     CDS             complement(4161..4757)
                     /locus_tag="ACZPCG_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284674.1"
                     /translation="MLGKKSPAVNTRSPRITGICGAGRSVGTTHLAVWSANYLASCCQ
                     KKTAVVEWSSHSDFVHLCLDDKNGQTARAFRLLEVDYYPQGEKEALASCIRSGYEEIL
                     IDFGPVRQEIRAEWFRCQIRILTASLSEWKLEAFLEFLAEEEELGKGWNCMAAFGSKA
                     IRHEIEKQFRIPIEKIPFSEDVFQVDKTAMAWFGKRFP"
     gene            complement(4730..6163)
                     /locus_tag="ACZPCG_06095"
     CDS             complement(4730..6163)
                     /locus_tag="ACZPCG_06095"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HIR1284675.1"
                     /translation="MIQKAGSGRTGTVWKARHLGLNEYRAVKCVPKYKVDHAAFCAEA
                     MVLKNLDHPGIPLVYDLEEDADYFYLIEEYLEGCSLYALIKDQGTLQEDLAVRYGLQI
                     CSLVEYLHHSCNQPILHLDLQPNNLIIWNDTVRLIDFDHAADRISANAARVRYGTEGC
                     AAPEQYTSDHPLDERTDIYAIGAVLRFMVKGTLKPDAENGLMLREPLEAVIRKCMEPD
                     MELRYQTAAETGKALGQLLAAEQAKGCLKRDQRKAEEKSISSHRIILTGNRPGAGVTH
                     LALGLCIYLKSHGVRVLYEERNRTGAVRNLAKALGGVRSGRDGILNMKGLDIMPWYGP
                     AACFEEPKRDFDIILKDFGTDWRSAGEDLKREGGVCLGIVCQSLWERGLTDRMAEELI
                     GLEPVLQKPYPLILIKRGTKRRGEKPGSLYRRAAVFASPYYEDPFHPDLVAAGFFEAV
                     WKEISLFLPAGKMEGGRRKCWGKSRRQ"
     gene            6377..7120
                     /locus_tag="ACZPCG_06100"
     CDS             6377..7120
                     /locus_tag="ACZPCG_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HIR1284676.1"
                     /translation="MKIERINENQIRCTLTSFDLSARNINIVELAYGTEKAKKLFREM
                     IQRASNEVGFEAEDIPLMVEAIPLSSESIMLVITKIEDPEELDTRFARFSPAAEETQE
                     EILPDPAGEFLEGAGDLGRLLSQHMADMTDSPQVPADSETASTKQIGKPLRIFLFEDL
                     DRAAEAAAAVADICTVANALYKKPGTRQYYLVLDRRDSDEHGFSRICNILAEYGKKLA
                     GDSAGEAYFKEHYETVIREDALKKLQLIR"
     gene            7446..8804
                     /locus_tag="ACZPCG_06105"
     CDS             7446..8804
                     /locus_tag="ACZPCG_06105"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HIR1284677.1"
                     /translation="MKTLLIKNVKHLVTCDTDDRVLNGVNVLVQDGIIVRIGQEDMTA
                     DDVIDASHMVMYPGLINTHHHLYQTFSRNLPQVQNMELFPWLKTLYEIWKNVNENVAY
                     YSSLTGMGELLKNGCTTCLDHHYVFPKGHNKGILDAQFAAADALGIRFHATRGSMDLS
                     VKDGGLPPDSVVQTVDEILSDCEEAVKKFHDPDPYAMHQVALAPCSPFSVSGELLRES
                     AKLARRLKVRLHTHLAETKDEENFTLSHFHMRPLEYMESLGWTGDDVWYAHGIHFTDE
                     ELKYLTRTKTGVAHCPISNMKLSSGIAKIPQMLELGVPVGLAVDGSASNDGSNLLEEM
                     RVAYLLHRLNSSNQAPGGYDILKMATAGSASLLGREKLGRIAPGMAADFFLVDLNRLE
                     LVGAQFDVTSMLCTVGLKSSVDYTVVNGNVVVKEGKLTGIDEEKVTEEANALVTDYLN
                     QP"
     gene            8835..10142
                     /locus_tag="ACZPCG_06110"
                     /pseudo
     CDS             8835..10142
                     /locus_tag="ACZPCG_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602686.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015851 - nucleobase transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease family protein"
     gene            complement(10139..11446)
                     /locus_tag="ACZPCG_06115"
     CDS             complement(10139..11446)
                     /locus_tag="ACZPCG_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016289508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1284678.1"
                     /translation="MGRFKPFAACVFPAVFAFALSGIYAIVDGFFVGRSIGDTGLSAI
                     NIAYPVTALIQAAGTGTGMGGAVMYSVRLAEKRDSEAENFMKGVFFYLALTGILLTVI
                     LFPLTDPLLSLMGADGELMKPGREYLSVIVLGSVFQVFGTGVVPLIRNQGKSVQAMYC
                     MIGGFVTNIFLDYLFVWVLRLGMAGAAWASVAGQAVTMAGGLLCMRGQKIPVGIPRQA
                     LSVFFSIWRIGLAPFGITMCPMISLLLMNRASLQYGGSEGVACYACIAYVITIMYLIL
                     QGVGDGSQPLMSRFYGEDNKKEVRITRTMAYAAAAVMGVALAVLLYWCRNGIGVLFGA
                     SETVSLAVGTDLYIFLAAVPFLAFLRVTTAGFYATEQTRFSYMIVYGEPIFLALLLPV
                     LPAWMGLKGVWWSQTGAQILTALMGWGLKLWSDRVHGYTADAV"
     gene            complement(11545..11745)
                     /locus_tag="ACZPCG_06120"
     CDS             complement(11545..11745)
                     /locus_tag="ACZPCG_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006782726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HIR1284679.1"
                     /translation="MKIEKTMLIGELIQMHELIPQMLMGAGMHCLGCPASQAESLEEA
                     CMVHGIDCDTLVSQMNEILADK"
     gene            12054..14162
                     /gene="pflB"
                     /locus_tag="ACZPCG_06125"
     CDS             12054..14162
                     /gene="pflB"
                     /locus_tag="ACZPCG_06125"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HIR1284680.1"
                     /translation="MRTEWNTFTPGVWQHEINVRDFIQKNYTPYDGDDAFLEGPTKNT
                     EALWNQVMELSAKEREAGGVLDMDTKIISTITSHGPGYLDKDKETIVGFQTDKPFKRA
                     LQPYGGIRMAVKACQDNGYEVDPEIVEFFTKHRKTHNAGVFDAYTPEMRACRSSHIIT
                     GLPDAYGRGRIIGDYRRVALYGVDALIEEKKEEKDSTRTIMYSDVIREREELSEQIRA
                     LEELKELGRIYGFDISKPAKDVKEAIQWLYFGYLAAVKEQNGAAMSLGRTSTFLDIYA
                     ERDLKAGTYTEQEIQEFVDHFIMKLRLVKFARTPEYNELFSGDPTWVTESIGGIGLDG
                     RPLVTKMSFRYLHTLKNLGTAPEPNLTVLWSTRLPEAFKRFCAKTSIESSSIQYENDD
                     LMRVTHGDDYAIACCVSSMRVGKEMQFFGARANLAKCLLYAINGGVDEISGKQIGPKY
                     RPVTSEYLDYDEVMDKYKDMMKWLAGVYVNALNIIHYNHDRYCYERLQMALHDKKVTR
                     WFATGIAGLSVVADSLSAIKYAKVKTVRNEKGIVTDYIVEGDFPKYGNNDDRVDQIAS
                     DLVHTFMSYIKGNHTYRGGIPTTSILTITSNVVYGKNTGSTPDGRKAGQPFAPGANPM
                     HHRDSHGAVASLASVAKLPFRDAQDGISNTFSIIPGALGKDDQVFMGDLEVELNGCAG
                     CEVGGNLFEGLDTEADSEEA"
     gene            14209..14454
                     /gene="grcA3"
                     /locus_tag="ACZPCG_06130"
     CDS             14209..14454
                     /gene="grcA3"
                     /locus_tag="ACZPCG_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA3"
                     /protein_id="HIR1284681.1"
                     /translation="MVKASEAQIDNLVSLLDGYAAEGGHHLNVNVFSKETLLDAQAHP
                     EKYPQLTVRVSGYAVNFNKLTKAQQDEVISRTFHDAI"
     gene            14527..>15044
                     /gene="pflA"
                     /locus_tag="ACZPCG_06135"
     CDS             14527..>15044
                     /gene="pflA"
                     /locus_tag="ACZPCG_06135"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HIR1284682.1"
                     /translation="MSGRVHSIESCGTVDGPGIRMVVFLSGCPMRCLYCHNPDTWDPK
                     GGTLTSVEELLAQYDKMKHFYTKGGITVSGGEPLMQIRFVTELFTEAKKRGIHTCLDT
                     SGITFHPENPAVLEQFDRLMAVTDLVLLDIKHIDPTEHEKLCSQPQQPVMAFESYLEE
                     KKIPVWIRHVVVP"
BASE COUNT         3823 a         4052 c         3237 g         3932 t
ORIGIN      
        1 taaattgata tctttttcca gctgaccctc tatccctact tttccgggat cccgtcctcc
       61 gtggccggcg tcaatcacga ctgccggccg taggcttcct gcccctcctt tgccgcttga
      121 aacggccaat gttccagaag ctacgaaggt tccggccttc caggaaagga gagccactgc
      181 acccagcatc aaaagcccca tcacggtctg ccatgtccaa tgtttcatac catttgctcc
      241 aaactgcccg ggcagttatt tttatgcctt tgatccagta tattcatcca gagaaaaacc
      301 tattcgcaga tgcgggatac tgctttactc tgactgcagc ttattataat ccctgacaga
      361 atccgctgtc atatattcca gatattcgta cagtacagca ggatagcaaa attcctgcca
      421 cattttaaat ccaggatctt ctactgcccc ctttaccgcc tcatagcctt ttccggcata
      481 ccaggctgtg ctcatcctgg aatatacctc tgccatggcc ttcttctgct cctctgtaag
      541 ctttacaaac agatctccct tttcctcctg tctggagttt ttcatcgcat cataggcctg
      601 attataaaag atatttttaa gagccgtttt actgtatgta ttgaaattga tcagattttc
      661 atctgtactc tgatgtctgc gggcccattt ttccatatct acctgtctgg tataatagga
      721 aaatgattcg tcatccctgt attccagcac cccatactgg cagggcggcg ttgtcaggga
      781 gctggtaacg atctcataaa ttccccttcc gtcctcatga cgcataaaat gctgcacatg
      841 aaggtgaccg cttaaaaaaa gagggatgtt ttccgcctca agccgttcaa tcagctcttc
      901 gctgtgctca atggtacaat cttctgtata aacgcggctt tgatccagaa gattgtgatg
      961 ccctacagga agcaggatca caccgtcctc tctggctttt tccagctgcc tgtcaatcca
     1021 ttcatacgtt tctgtcccaa tcattccgcc aatcttgctt accggcgaat actggcaggt
     1081 atccagcatc agaaggcgca tggaggggct gagatcatag gtgtagctta aggaataagg
     1141 atcgcgctct gaagcctcat tatagccaaa ctgtgcatag atactggcaa atccacctgc
     1201 gtctatggtc tggaccggct ctctgttttt tccaatatag gaggatgccc atcggctgtt
     1261 gatatcatga tttcccggta tcacaagtac gctgatgcct ctgcgctcta cctcatccaa
     1321 aagagcagca aattcctcat ggctctttac ttctccattt aatgtcagat ccccgctcac
     1381 gatcagcaca tccggcgtaa gcagctcgat ctcatcaaaa gccgcatcta cgatttccca
     1441 tacataatga gtcagctttc catccccatg ttcaatttca ttttgaaagg agcttccccg
     1501 gtctgtaaga gatcttgcca gataatggac atctgtcatc aggacaattc tctctccatc
     1561 ctccatcggt tctgctttcg tttcccgttc tgcatccggg aaaggcggaa tggattccgg
     1621 ctccgttttt gtgggctttg tctgactgct ttcctccgtt tcctgtgagg aagcagacgg
     1681 gaccactaat ccatcctctc ccatagtttc actttcctcc ctgacatctc ccggcgcccc
     1741 tgtaaatata ctgcatcccc cgattcccac accagccaga cacacaagag ccacggcgat
     1801 ccatctcttt cccttcataa catttccccc tcgtcataca gccttaacta ttgactgttt
     1861 ctattttacc tgagacacga tttcccagat ggagcgatcc tcaaagccaa gcttccagca
     1921 ggctgctccc gccagatcat attcattgat cagatcgacc ttaagcccca gcgacttctc
     1981 ctcctccatc cagacggact gtactccgtt ttcattggag ctctccccat aatatactcc
     2041 caggtcatcc agccattcca gttctatatt gttttcttta atccagttct ttgcatcccg
     2101 gatcccatag gccttggatg tggtttttcc atccttttcc gtccatatcc ttgtataaaa
     2161 aggaacactg ttgatcacct tttccttcgg aacttctttc agcgtatctg caatgccctt
     2221 ttctacatag ggaagggatg ccacgggacc tgcctcgccg ccggcataat gctcatcata
     2281 ccccataacg atcacataat ctgctacgat cccctgctct ctcctgttat aaaaggcggt
     2341 atatgctgat ggcacatagt tatccaccga aagcaccagc cctttttttc tgcatgccac
     2401 agacatttcc cggataaact gaacataatg gggaccggct tctgccttca ggctttcaaa
     2461 gtccagattg aaaccgtcaa acccataggt atctgcctct ttcatcagat tttcaatgag
     2521 ttttctgcgg ttagaggtca cagacataag cttctttgta tccagctctt tcacgctctc
     2581 tttagtgctc acattttcca ccatagccca taccttaagc cccatatcat gggcctgttc
     2641 cacataatcc ctgctggcaa gagaagtgta agtgccgtca tttccaatca cattaaacca
     2701 ggtgggaaca atcacattca tcccctctgc cgtctgtgca tattccttaa gagtgctgtt
     2761 tgcttccttt ctggtaacct gatggaatcc cagacgcacc ttttcatcca tggaaatgct
     2821 tgtataaact ggcgcttcaa accgaccttg aggcgtgatc tcctgttcct tttccagacg
     2881 ccggttctgc acatatccga tataaccgtc ttccgttctc actttagacc atttatccat
     2941 agattccagc acttgaaccg tacttttttc agaaagctct gtaatgatgt cgctcttgat
     3001 tccgcccttt accctgagga tacttttttt gcggacctga gcggttttta cagtccccca
     3061 taccgtgtct ataaatacac gtttgatctg gctggtatca aaggattgtt gacggatatc
     3121 tgaataattc atgatcagtc ccaacgacag gtatgcttcc ccttctttta ccttcaaaag
     3181 aggcccctgt tctcccatat cggactcatc cgcgtataca atttcctccg gcagcgcata
     3241 taccagcagc ttttccgttt catcccagta aaaccgctca ttcacatatt cgtttaccca
     3301 gcttatgggc agatatacct gtccatcttc ataaattcct ttttcttcct gaagctcatt
     3361 atttaaaagg atcgccacct gactgccctt tatatgaaat aactctgcct tatccgccat
     3421 ctcttttccc ggcatatacc gtcttccgat cacaaagcct gccgctgctc ccgcaatcag
     3481 aagaaggaga aaaaccaccg caagaagcgg gccgaaactg cgtctttttc ttcttctgct
     3541 cctcattttt tcctccgatc ctgtctgtgc gccccaaaag gccccgtccg tatatctggt
     3601 ttctatttta tcatagaatc cccggaaaga ggagcaaaaa atatgtgcac gcaaaaatac
     3661 cgccggtaag ctgctgcctg cttgcaggcc gggacggtat cttcttgtcc catacggcag
     3721 cagcttccgt cggtatctga atatgtacag gctattgcag cctcttttgt tatcttctgg
     3781 tttgtccgga atctgtcaag tatactcggt caaaacggat ttcctttaag ctgccctgtt
     3841 ctgagcagat aaaatccccc atgtagaaac ctccgtcctc tcccgtttca aaaatgcggc
     3901 tccattcttc ttccagacat tccatcccat ctaccagaac agaaactccc ttatctctga
     3961 atcgcctgag accatccaga tagttcattt gctgtgtcct gacttctcca gattctttac
     4021 catacattat ataacctccc tgcagatgtt cctttcttac agtctggagg caaaacgtga
     4081 catatactcc ctttaagaaa aaaacaactg ctgatattct cttttgcaac ggaaaccggt
     4141 tttcttctgt acaggccgaa tcatggaaac cgttttccaa accacgccat ggctgtttta
     4201 tctacctgaa atacatcctc tgaaaacgga attttctcta tcggaatccg aaactgcttt
     4261 tctatctcat gtcttatggc cttactgcca aaggcagcca tacaattcca tccttttccc
     4321 agttcttctt cctctgcaag aaattccaaa aatgcttcaa gcttccattc tgacagggat
     4381 gctgtcagaa tccgtatctg gcaccggaac cattctgcgc gtatttcctg tctcacgggg
     4441 ccaaaatcga tcagtatctc ttcatatccg gatcggatac aggatgccag agcttccttt
     4501 tctccctgtg ggtaataatc tacctccaac agccgaaaag ccctggcagt ctgtccattt
     4561 ttatcatcca gacacaggtg gacaaaatca ctatgggaac tccactccac caccgctgtc
     4621 tttttctgac agcagcttgc cagataatta gcgctccaaa ctgccagatg agtggttccc
     4681 acagaccttc ccgccccgca gataccggtt atccttggac tgcgcgtatt tactgccggc
     4741 gactttttcc ccagcatttt ctccttcccc cttccatttt cccggcaggc aggaaaagag
     4801 aaatttcctt ccagacagcc tcaaaaaaac ctgccgccac caaatcagga tgaaacggat
     4861 cttcataata tggactggca aatacagctg cccttctata caggctccca ggcttttctc
     4921 ccctcctttt tgtcccgcgt tttatcagga tcaagggata tggcttctgc agcaccggtt
     4981 ccagtccgat cagctcctct gccatcctgt ctgtcaatcc cctctcccac aggctctgac
     5041 agacaattcc aagacagacc ccgccttctc tttttaaatc ctctccagcg cttctccagt
     5101 ctgttccaaa gtcctttagt ataatatcaa agtcccgttt cggctcctca aaacaggccg
     5161 ccggaccata ccagggcatg atatcaagcc ccttcatatt caggatcccg tcccttccgc
     5221 ttcgaactcc ccccagagcc tttgcaagat tccttacagc ccctgtacga ttcctttcct
     5281 catacagcac gcggactcca tggcttttta gataaatgca aagccccagg gccagatggg
     5341 tcacacctgc tccgggccgg ttccccgtca agatgatcct gtgagatgat atcgactttt
     5401 cttctgcttt cctctgatcc cttttcagac acccctttgc ctgctctgca gccaaaagct
     5461 gtcccagagc ttttcctgtc tccgccgcag tctgataacg cagctccata tccggttcca
     5521 tgcattttct gatcaccgcc tccaaaggtt cccgcagcat gagtccgttt tcagcgtccg
     5581 gcttcagggt tccctttacc ataaatcgaa ggaccgcacc aatggcatag atatctgtcc
     5641 gttcatctaa cggatggtct gaggtatatt gctcaggcgc tgcacagcct tctgtgccgt
     5701 atcgtactct ggcggcattg gcactgatcc gatccgccgc atgatcaaag tcaatgaggc
     5761 gcaccgtatc attccagatg atcagattat tcggttgtag atctaaatgt aaaatgggtt
     5821 gattgcaaga atgatgtaaa tattcaacga gagaacagat ctgcagtcca taccgaactg
     5881 ccaaatcttc ctgaagtgta ccctgatctt ttatcagggc atatagagaa cagccttcca
     5941 gatattcttc aatgagataa aagtaatctg catcctcctc caagtcataa accagcggaa
     6001 tccccggatg atcgagattc ttcagcacca tggcctccgc gcagaaagca gcgtgatcca
     6061 ccttgtactt gggcacacat ttaaccgccc ggtactcatt cagccccaga tggcgggcct
     6121 tccagactgt acctgtacgt cctgagcctg ctttttggat caattcatac ttaccaaaca
     6181 gtatctcctt ctctgtctcc ccctcctttc gttacaatat catctcatgt ggaaatgtta
     6241 ctctattttt tgtaacttgt ctatggtcga gaggataacc accgaaaaac agttgatatt
     6301 tccccctgtt atgtagtata tttatgttgt agaagtttac ccatttttaa atatatacag
     6361 aacaggaagt gatccgatga agatagaacg aatcaacgaa aatcaaatac ggtgtacgct
     6421 caccagcttt gacttgagcg cccgtaatat caatattgtc gaactggctt acggtacgga
     6481 aaaagcaaaa aagctgttcc gcgagatgat tcagcgcgct tccaatgaag taggttttga
     6541 agcagaggat attcccctga tggtagaggc cattcctctt tcttctgaaa gcattatgct
     6601 ggtgatcact aagatagagg atcccgaaga gcttgacaca cggtttgccc ggttctctcc
     6661 tgctgcagaa gaaacacagg aagagatcct gcctgacccg gccggagaat ttttagaggg
     6721 agccggtgat ctgggacgcc ttctttccca gcatatggct gatatgacgg acagccctca
     6781 ggttcctgct gatagtgaaa ccgcttccac aaaacagatc ggaaaaccgc tgaggatctt
     6841 tctatttgag gatctggatc gtgcagcaga agccgcagcc gctgtcgctg atatctgcac
     6901 agtcgccaat gcactttata aaaagcccgg aacacgccag tattatctgg tattggatcg
     6961 cagggattca gatgaacacg gtttctcaag aatatgcaac atactggcag aatatggcaa
     7021 aaaactggcc ggagattctg caggtgaagc ctactttaag gaacattatg aaaccgtcat
     7081 ccgggaggat gcgcttaaaa agctgcagct catccgataa cagcctatga tccgaaggcc
     7141 cttgcaggcc aaacatacgt taagagcagt tcctcacagg gagctgcttt tttctgacca
     7201 atttttgtta gatattacca atttttttga ggtttattct gtacttattg acattgtatc
     7261 tcccattttt gattgttata attataacac acacggcaga ctgtcgtgga agcatcgttc
     7321 tttgacaaga tcatatctat atagctgtct aaaacatagc tgggtttatg gtttcccttt
     7381 taccgccagc agtataccag tcatatagtc aatgatgcac agatgatttt aaggaggtca
     7441 gtgatatgaa aacactgctt atcaaaaatg taaaacatct tgtaacatgt gacacagatg
     7501 accgcgtact gaatggcgta aatgtgttgg tacaggatgg aatcattgtc cggatcgggc
     7561 aggaggacat gacagcggat gatgtgatcg acgcatccca tatggtgatg tatcccggtt
     7621 taatcaatac acaccatcat ttatatcaga cattcagtcg gaatttacct caggtacaga
     7681 acatggagct gtttccatgg ctgaaaactt tatacgaaat atggaaaaat gtaaacgaga
     7741 acgtagcata ctacagttct cttaccggta tgggcgaact gcttaaaaat ggctgtacta
     7801 cctgtctgga tcaccactat gtattcccaa agggccataa taagggaatt ctggacgccc
     7861 agtttgcagc cgccgacgcc ctgggcatcc gtttccatgc caccagagga agcatggatt
     7921 taagcgtaaa ggatggaggg cttcctcctg acagcgtggt tcagaccgtg gatgaaatcc
     7981 tctccgactg cgaagaggcc gtaaagaagt tccatgaccc ggacccttat gccatgcatc
     8041 aggtagctct ggctccctgt tctcccttca gcgtatccgg agagctgctg cgcgaatctg
     8101 caaagctggc ccgcaggctg aaggtacgcc tccacaccca tctggcagag acgaaggatg
     8161 aggaaaattt caccttaagc cattttcata tgcgccctct ggaatatatg gaaagcctgg
     8221 gctggacagg ggatgacgta tggtatgctc acggaattca ttttactgat gaagagctta
     8281 aatatctgac ccgaaccaaa accggagttg cccactgtcc gatttcaaac atgaagctgt
     8341 cctcaggcat tgcgaagatt cctcagatgc ttgaactggg tgttcccgta ggccttgcgg
     8401 tagacggctc cgccagcaat gacggctcca acctgctgga ggaaatgcgc gtggcctacc
     8461 ttctccaccg cttaaacagc agcaatcagg cccccggcgg atatgatatc ttaaaaatgg
     8521 caactgcagg cagcgccagc cttttaggcc gtgaaaagtt gggacggatt gctcccggta
     8581 tggctgccga cttcttcctt gttgacttaa accggctgga gctggtgggc gctcagtttg
     8641 acgtaacctc catgctctgc accgttggtt taaagagcag cgtggattat acggttgtaa
     8701 atggaaatgt tgtggtgaaa gaaggaaagc tcaccggaat tgatgaagag aaagttacag
     8761 aggaggcaaa tgctcttgtg actgactatc tcaatcagcc ataaggccac agtccacaga
     8821 aaggaaaagg taaaatgagt cagaagcaat acgctaaagt aacagaagta aacggcatcc
     8881 cgcggttttc agaggccctt cctctggccg cccagcatgt agtcgccatg attgtgggct
     8941 gcgttacacc ggccattatt ctttccggag tcaccggatt aagcgcatcc gatcaggtcc
     9001 gcttaattca ggcctctctg ctgatctccg ggcttgccac actcatatag ctctttccac
     9061 ccttccggct ctgcggctca gggcttcccg ttatcatggg cgccagcttt gcccatgtgc
     9121 ccctgctgat tgccctggga gcacagttcg gaatgcccac catattggga gcacagctgg
     9181 caggcggctt tgtggcaatc cttgtaggat tattttataa taagctaagt aaaattttca
     9241 ctcctctggt aacgggaaca gtggtattta ccatcagatt ttccctgtac cctaccgcca
     9301 ttacctatat ggcggtaggc tccggagcgc cggatttcgg aagcgtaaaa aactgggctc
     9361 tggcaatctt tacactggta attgtaacct tttttaacta ttttacaaaa ggaatctgca
     9421 agatggcctc tatcctcatc ggcattgtct gcggctatct ggcagcactt gcgctgggaa
     9481 tgatttcctt tgaaagcata ggcgaggcca gctggttcca gttcacccca cccttatact
     9541 tcggaatgaa atttgacatt acagccatca tttccatggc gatcatgttt gtggtgggct
     9601 ctctggaaat catgggcgac tttacttcaa ccgcaggcgg cggattaaac cgtccggttc
     9661 aatctgaaga aatgtccgga ggcatcatcg gaaacggtat catcagtatc atcgactccc
     9721 tcttcggcgg gcttcctact gcaaccttca gccagaacgt aggtattgtc atcatgacaa
     9781 aggtcgtaaa ccgcgtggtt ctgggactgg ctgcagtgat aatcgtgctg gcaggactga
     9841 ttcccaagtt ttctgccctg ctgactactg ttccctcctg cgtactgggc ggcgctaccg
     9901 tatccgtatt tgccatgatc gccatgaccg gcatcaagct tgtggtaaaa gaaggactta
     9961 ataccagaaa tacagccgtt gtaggcttat ccgttgccct gggcatcggc gtaatccagg
    10021 tagcagactg cttcgcacag ttcccggaat gggcgaaaac cattttcgga acctctcccg
    10081 tagtaatcgc tacccttact gcagtgacac tgaatctgtt gctgccaaag gatctggatt
    10141 aaaccgcatc tgcagtgtat ccatgcaccc ggtctgacca gagcttaagc ccccagccca
    10201 taagggccgt aaggatctgg gctccggtct ggctccacca gacgcccttt aatcccatcc
    10261 aggccgggag cacaggaagc agaagggcta aaaatatggg ttctccataa acgatcatat
    10321 aggaaaatcg ggtctgttct gtggcgtaaa agccagcagt tgtaactcgc agaaaggcca
    10381 gaaacggcac cgccgccaga aatatataca gatccgttcc aactgccaga gatacggttt
    10441 cggatgctcc aaacagaact ccgatcccgt ttctgcacca atacaaaagc actgccagcg
    10501 ccactcccat gacggcggcg gctgcatatg ccatggtccg cgtaatgcgg acctcttttt
    10561 tattatcctc gccgtaaaag cggctcatca gaggctgact cccatccccc accccctgaa
    10621 ggatcagata cataattgtg atcacatagg caatacaggc atagcaggcc acaccttcag
    10681 aacctccgta ctgaagggac gcccggttca taaggagcag cgaaatcatc gggcacatgg
    10741 taattccaaa gggagccagc ccgatccgcc aaatgctgaa aaaaacggaa agcgcctggc
    10801 gcgggatccc caccgggatt ttctgccctc tcatacacaa aaggccgcct gccatggtga
    10861 cggcctgtcc tgccacagag gcccaggccg ctcccgccat tccaagccgt aaaacccaga
    10921 caaagagata atctaaaaaa atattagtca caaaaccgcc gatcatacag tacatggcct
    10981 gtaccgattt tccctgattg cggatcagcg gtacaacgcc ggttccaaag acctgaaaaa
    11041 cagaacccag aacaataacg gacagatatt cccgtccggg cttcatcagc tctccatctg
    11101 ctcccataag ggacagcaga gggtctgtta agggaaataa aatgactgta agcagaatgc
    11161 ctgtaagggc aagataaaaa aagacgccct tcataaaatt ctccgcctcg ctgtccctct
    11221 tttctgccag acgaacggaa tacataaccg ctcctcccat accggtccct gtacccgctg
    11281 cctggatcag ggcagtgacc ggatatgcaa tattgatggc cgaaagtccc gtatctccaa
    11341 tgctgcgtcc tacgaaaaat ccgtctacga tcgcatagat cccggagagc gcaaaggcaa
    11401 atacggccgg aaacacacag gctgcaaagg gtttaaaacg ccccatttct ccaaatacct
    11461 cctatcgtaa aaataagact gccttaaatg ccccaaaagc atcttaaagc agccctatcc
    11521 tgtctccgca tatgcagacc tgtcttactt atcagcaaga atttcattca tctgggatac
    11581 cagggtatcg cagtcaatcc catgtaccat gcaggcctct tccagagact cagcctgaga
    11641 agccgggcag cctaagcagt gcataccagc gcccatcagc atctgaggga tcagctcatg
    11701 catctggatc agctcgccga tgagcattgt cttttcaatc ttcatctgta ttttcctcct
    11761 tattatctgt aatactttca tattaaaagt attctgcgca tttttattcg cttttatcag
    11821 cataatacat tttgtttttt ttggcaagcc ctgtattttc ctgtatcatt tttcaatttt
    11881 tctggtatag tattgtttgt ctaaagttat gtattttttt ggatacagta tacaatttta
    11941 ttgatttgtt gcatttgcaa cagatttttt catgaaatta tagtagacta actttattca
    12001 aagagatacc acaaaaagta tccccaaatc acattttaca ggaggaaacg tccatgagaa
    12061 cagaatggaa cacatttaca ccaggtgtat ggcagcatga aatcaatgtc cgcgacttca
    12121 tccagaaaaa ctacaccccc tacgacggcg atgacgcctt ccttgaaggg cctacaaaaa
    12181 atacagaggc tctctggaat caggtaatgg agctctctgc caaagagcgt gaggccggcg
    12241 gcgtactgga tatggatacc aagatcattt ccaccattac gtctcatggc cccggatacc
    12301 tggataaaga taaagaaacc attgtgggct tccagacaga caaacccttt aagcgggcgc
    12361 tgcagcctta cggcggtatc cgcatggcag taaaggcctg ccaggacaac ggctatgagg
    12421 tagaccctga aatcgtagag tttttcacaa agcacagaaa aacccacaat gcgggcgtat
    12481 ttgacgccta caccccggag atgagagcct gccgttccag ccacatcatc accggtcttc
    12541 cggatgccta cggacgtgga cgcattatcg gcgattaccg ccgtgtagcg ctttacggtg
    12601 tagatgctct gatcgaagag aagaaagagg aaaaggattc taccagaacc attatgtact
    12661 ctgatgtaat ccgtgagcgg gaggagcttt ccgagcagat ccgtgcactt gaggaattaa
    12721 aggagctggg acgcatctac ggctttgata tttcaaagcc tgcaaaggat gtaaaggaag
    12781 ccatccagtg gctgtacttc ggctatctgg cagccgtaaa ggagcagaac ggcgcagcta
    12841 tgtccctggg acgcacctca accttcttag atatctatgc cgagcgcgac ttaaaggcag
    12901 gaacgtacac cgagcaggaa atccaggagt ttgtagacca ctttatcatg aagctgcgtc
    12961 tggtgaaatt cgcccgtact cctgagtaca atgaactgtt ctccggcgat cctacctggg
    13021 taactgagtc catcggcggc atcggcctgg acggacgtcc tctggtaaca aagatgtcct
    13081 tccgctacct gcacacctta aagaacctgg gtactgcgcc tgagccaaat ctgaccgtac
    13141 tgtggtccac aaggctgcct gaggcattta agcgcttctg cgcaaaaacc tccattgagt
    13201 cttcctccat tcagtatgaa aatgacgacc tcatgcgtgt aacacatggt gatgattacg
    13261 caatcgcctg ctgtgtttcc agtatgcgcg tgggcaaaga aatgcagttc ttcggcgcaa
    13321 gagctaacct ggcaaagtgt ctgctctatg ccatcaacgg cggcgtagac gagatctccg
    13381 gcaagcagat cggacctaag taccgccccg ttaccagcga atacctggat tatgacgagg
    13441 taatggacaa gtataaggat atgatgaaat ggctggcagg cgtttatgta aatgccttaa
    13501 acatcatcca ctacaaccat gaccgctact gctatgagcg cctgcagatg gctcttcatg
    13561 acaagaaggt aacacgctgg tttgcaaccg gtattgccgg cctgtccgtt gtggctgact
    13621 ccttatctgc catcaagtat gcaaaggtaa agaccgtccg caacgaaaag ggcattgtaa
    13681 ccgattacat tgtagaggga gatttcccga aatatggaaa taatgatgac cgggtggatc
    13741 agattgcttc tgaccttgta cacaccttta tgagctatat caagggaaat cacacctaca
    13801 gaggcggtat ccctaccact tccattttaa ccattacctc caatgtagtt tatggaaaga
    13861 ataccggctc tactcctgac ggaagaaagg ccggacagcc ctttgcacca ggcgccaacc
    13921 ctatgcacca cagagacagc cacggcgcag ttgcttccct ggcatctgta gcgaagcttc
    13981 cattccgtga tgcgcaggac ggtatttcca acacattctc cattattcca ggagcacttg
    14041 gaaaggacga tcaggtcttc atgggcgacc tggaagtaga attaaacggc tgcgccggct
    14101 gtgaagtggg cggaaacctt tttgagggtc tggacacgga agcagacagc gaggaagctt
    14161 aagcagcatt taaccaactt tatttttaag aaagcgagga caatcactat ggtaaaagca
    14221 agcgaggcac agatcgacaa cctggtatcc ctgttagacg gatatgccgc tgagggagga
    14281 catcacttaa atgtaaatgt attttccaaa gagactcttc tggacgctca ggctcatcct
    14341 gaaaaatatc ctcagctcac tgtgcgcgtt tccggctatg ccgtaaactt caacaagctc
    14401 acaaaggcac agcaggacga ggttatttcc agaaccttcc acgacgctat ttaatcatat
    14461 gatgaccgaa caggcagatg cattccggca tctgcctgcg gcttttaaga aacggaggtt
    14521 ttcattatgt caggacgggt acattccatt gaaagctgcg gtacggtaga cgggccggga
    14581 atccgcatgg tagtattttt aagcggatgt cccatgcgct gcctttactg ccataatccc
    14641 gatacctggg atccaaaggg cggcactctc acctcagtgg aggagctttt agcccagtat
    14701 gataaaatga agcatttcta taccaaaggc ggaatcacag tcagcggcgg ggaacccctg
    14761 atgcagatcc gctttgtaac agagcttttt acagaggcaa aaaaaagggg catccacacc
    14821 tgtctggaca cctccggcat caccttccat ccggaaaatc cggctgtcct ggagcagttt
    14881 gaccgcctga tggctgtaac cgatctggtg cttttagata tcaagcacat tgacccgacg
    14941 gaacatgaaa agctctgcag ccagccccag cagccggtga tggcctttga atcttatctt
    15001 gaggaaaaga aaatccctgt atggatccgc catgtagtgg ttcc
//
LOCUS       DBMDUH010000052        14802 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_2221_length_14802_cov_4.301408, whole genome shotgun sequence.
ACCESSION   DBMDUH010000052 DBMDUH010000000
VERSION     DBMDUH010000052.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 14802)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14802)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14802
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2221_length_14802_cov_4.301408"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(74..1627)
                     /locus_tag="ACZPCG_06140"
     CDS             complement(74..1627)
                     /locus_tag="ACZPCG_06140"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purple acid phosphatase family protein"
                     /protein_id="HIR1284683.1"
                     /translation="MKKKRVMAGILVGALAVGGHQVWAATVHYNDSSVTGGSAEWQAW
                     TRQWEQLANDYTQVSLTPGAAASQLNFAWYSEGENATPVVYFGIDADDLKAWEGQASG
                     VDTNLTGGKVYSYNYVTVDGLKENSTYYYSVEKNGVRTPAEVYKTGSFSNVNILYVGD
                     PQVGASKGQPQGEGKLSADPGAANTAARNDGFGWDRTLDIALAENPDLNFVISAGDQV
                     NKTGKPKEEEYAAYLSASALKSLPVATTIGNHDSLNKDYSYHFNNPNPTNLGMTEAGG
                     DYYYTYGPGLFIVLNTNNYNVAEHEQAIKQAVENFPDTKWRIVTIHQDIYGSGLDHSD
                     TDGMILRTQLTPVFDKYDVDVVLQGHDHTYSRSKLLYGDGKTHGAYEFRLDETGSDYD
                     WDHAYNVNTDESIALYPEDEPSAALKEAFAQDNHCYTIEGSGQSVVTNPKGTLYMTAN
                     SASGSKYYELTNTQQDYIEVRSQNWLPSYSVINLTYDDFKITSYQITDNGKVETIDNT
                     FTIHKTGSR"
     gene            complement(1742..2599)
                     /locus_tag="ACZPCG_06145"
     CDS             complement(1742..2599)
                     /locus_tag="ACZPCG_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015514337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016852 - sirohydrochlorin
                     cobaltochelatase activity [Evidence IEA];
                     GO_process: GO:0019251 - anaerobic cobalamin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin cobaltochelatase"
                     /protein_id="HIR1284684.1"
                     /translation="MNLKTVSEIKGTNQNKTGERELLAVSFGTSYKNTRERTIEAIER
                     DMESAMPEYILHRAFTSQIIINRVKQKENLVIDNVRDALKHAVENGVKTLIIQPTHMM
                     KGFEYQDLAEEASEYRALFEYMAVGEPLLTSEEDFETVIRAITEDTKEYDDGETAVCF
                     MGHGTEAEANQVYKKLQSILLQKGFTNYYIGTVEAEPSIEDVLREVKKGSYKKVVLQP
                     LMIVAGDHANNDMAGDEEGSWKREFERAGYEVSCVLKGLGEIELIRHLFVSHAKAAGE
                     LFLPETGKE"
     gene            complement(2672..4012)
                     /locus_tag="ACZPCG_06150"
     CDS             complement(2672..4012)
                     /locus_tag="ACZPCG_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007705688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR1284685.1"
                     /translation="MKTIITNSIIITMNSDMEVFPDGYVKIEGTKILETGPSCDLKET
                     TEELRAAGWQIKDGKRGILMPGMVNTHCHLGMIPFRGLGDDCKDRLRVFLLPMEQKAM
                     SRRMAALSSRYAICELLLSGVTSVMDMYYFEAAVAQVMDQTGIRGIAGQTLMDEGGCD
                     ASDADQSLSMAKELIETYRMHPRISGCAAPHATNTCSPQILKKAWELDTANQVPFTLH
                     AAEMDYEMTWLAQTRGCTPVGYLDQLGVLSDQTAAAHCIHLTDRDIEILRQRGSGVCH
                     CIGSNAKAAKGVAPVLKLLKEGVSVGLGTDGPASGNTLDLFTQMRLCAGFQKNDSHDR
                     SAMPAKDIVSMATRQGARVLGLEEKTGSIEPGKEADLVLVETDSPNMFPVYDPYSALV
                     YSANASNVRDVYVAGTCLVQDKKLVYEDFESIRRELSDCMGRSAFGEMQELHRL"
     gene            complement(4015..5733)
                     /locus_tag="ACZPCG_06155"
     CDS             complement(4015..5733)
                     /locus_tag="ACZPCG_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007705686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase C-terminal domain-containing
                     protein"
                     /protein_id="HIR1284686.1"
                     /translation="MSPDYIIRNVNIYQTFRQCFELRDAAVKDGRFYAVLPVNSGVYP
                     EGVPVYDAQGRYMIPGLIDIHMHIESSMTCPSRFGPLALSHGTTTVVADAHEIANVFG
                     MKGIAEFQSQKTEMDIFWAIPSSVPATDTALETSGATITPEDAAALLEDRNILCLGEV
                     MNFKQLAPEDDRDTPIRQMIRLCRKKRGRDIRLEGHCPRISGADLNRFIYEGIDADHT
                     QQTPESVLEKTDLGMFLELQRKSLTPEVVETLNHYNLYENTALVTDDVMPDQLLKGHL
                     NQIVRTAVLAGMPVEKAIYCATYTPARRMHLDDRGMIGPGKLADFVFLKDLDTMEPEA
                     VVKEGRLAWIEGDSRCGMEEETNFPEHFYHSVHCRPAVLEDFILKAPVKFGHVTANVM
                     QINTFGTATRHIKRQLPVKDGIVCWQEAGLSLAVLYERHGKNGNIGYGLVEGAFAKKG
                     AAATTWCHDSHNLLVLGTSPEDMVLAQKRILELEGGYCIFSDGCCLGQVQLNVAGIIN
                     EGSLEKLSCELGQIRKSMASLGYKNNNEIMSMSTLALPVSPALKLTDRGLLDVKTQKQ
                     VELIEV"
     gene            complement(5747..6802)
                     /locus_tag="ACZPCG_06160"
     CDS             complement(5747..6802)
                     /locus_tag="ACZPCG_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006771337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1284687.1"
                     /translation="MKKRVLAAVCMAAMAVTACSGGSEGEGAQAGGKQKLVLSTYGLS
                     EDISAEEVYAPFEEQFNCQIVTETGGTNDRYTKLKADSETTIDVIELSQAMAAKGAEE
                     GLFETIDLSKIEHASDLIAAAKTLADNGQGVPYTINSIGIMYNPEAVGFEITGFDDLW
                     KPELAGMVSIPEITTTFGPAMVYMASDHAGVDIKTDNGEAAFKALEELKPNLVKTYAK
                     SSDLINMFTSGEVAAAVVGDFGVPTIKAANPDLVYVTPEGTYANFNTISITKNCQNKE
                     LAYEYLNYRLSAELQSKTGKALNEAPTNKTVTFTEEESQNMTYGPAAEKAKVLDYGFV
                     NPLLNQWIDQWNRIINN"
     gene            complement(6842..7882)
                     /locus_tag="ACZPCG_06165"
     CDS             complement(6842..7882)
                     /locus_tag="ACZPCG_06165"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007705681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1284688.1"
                     /translation="MAFMTLEKIGVSYDKKKQILKGLDLSVEKGELVSLLGPSGCGKT
                     TTLRVVAGFIEPGEGTFTLDGEDMTKVPVHKRNFGIVFQSYALFPHLTVYDNVAFGLR
                     TRKMDKAQIDRKVKEILEVCGLTELSGRFPREMSGGQRQRVALARALVIEPKLLLLDE
                     PLSNLDAKLRISMRVEIKRLQKKLGITTLFVTHDQEECFSISDKVAVMNGGIIEQYDT
                     PEQIYRQPATEFVARFIGFENFLNLERCGDGSYLAGGQSRFCSFMECPEGEFTGTIRP
                     DDIRIAAPDQQENVITGRIGVRTFLGKSYQYEVETAAGVLKVNMNTECIYAEGDQIRL
                     YLPEDKLILVRR"
     gene            complement(7895..8677)
                     /locus_tag="ACZPCG_06170"
     CDS             complement(7895..8677)
                     /locus_tag="ACZPCG_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015552827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1284689.1"
                     /translation="MKKNRWLTVYAVLVFLFLMIPLVIITVTAFGGGSAITFPIDSFS
                     LKWFGNVFALKSFRRSFLTSLEVAFLATFIALLVGIPAAYALARSGIRGKQLLRSVFL
                     SPTIVPGIVVGFILYQFLILTFRLPVMASLLAGHFLVTLPYVIRVVGSAMEQFDFSIE
                     EAAWSLGCPKGAAFFKVVLPNVTSGISSAFMLAFINSFNNIPVSMFLTGPGVSTFPAT
                     LMNYIEYNYDPTVSAVSVLLMAATVLIMVIVDRTLGIASLAK"
     gene            complement(8677..9504)
                     /locus_tag="ACZPCG_06175"
     CDS             complement(8677..9504)
                     /locus_tag="ACZPCG_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002600806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1284690.1"
                     /translation="MKKSSTWMMLAPGLIILLLCLGLPLISVLAPTFTAGQYPLSAYV
                     EFFQDEYYRKILFRTLKIAVITTGICMAGGIPTAYFISRCDKKWRGLLLAASIFPMMT
                     NSVIRSFAWINILGSNGIINRILLALNVADKPMKLLYTDFAIIIGSVYLFLPLMIVTV
                     TGVMENIDDDMMEAAQSLGAGRFEAFMKVIFPMSLPGIIVGGILVFTGTLTAYTTPQL
                     LGGNSNMVLATLIYQRAMSVGDWNGASAVALIMIVITLIVIRGFNALAARLDKRGGK"
     gene            9657..10583
                     /locus_tag="ACZPCG_06180"
     CDS             9657..10583
                     /locus_tag="ACZPCG_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007705672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1284691.1"
                     /translation="MDLKEARYILAIAHYKSIGKAAKALFISQPSLSKYLKNLEQQLG
                     SPLFSRLEQGYIPTYMGERYLYYAEQIAAFGQEWDQEYEDITRRAHGRLNIAIPIMLG
                     STLLQPTLMEFHRRYPHVTINIMEEVSFVAEQTLKNTSIDLTIYNVHTFPKLLDYQVI
                     RTEEIVLILPEGHPLEKEAEKKEGFSHPWLDLKKLSQENFILLYPDQNTGGIARDLFA
                     RHEINPPVLLHTRNSQMSIKLAMDGMGAAFAPESYFNYMASLRSDRPGCYCVGEKPVL
                     TTTIAACRPGRYMPKHVKDYIDLLKIYGNSSS"
     gene            complement(10580..11419)
                     /locus_tag="ACZPCG_06185"
     CDS             complement(10580..11419)
                     /locus_tag="ACZPCG_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7402 domain-containing protein"
                     /protein_id="HIR1284692.1"
                     /translation="MTLICSWIPAEAKETKEDQISIRVSDWKGHTKAFAQDPEESILA
                     FKDVYEPGDIIQFHFPETEGYYMVRVDDTMDEALVYMTKKDLTFEVPFDEKKFSYNPK
                     SFTGVRHYLTCRKAASWETGIYRNLAKNVLDQHGDRGCYPHASANVETRGESVFAARN
                     AIDGVTATLSHGKWPYESWGINRRDDAELLLEFGRPVDIDQVRLWTRADFPHDNWWVS
                     AVLTFSDGTREVVNMEKSEKAHTFAITKKGITWVRLSDLIKADDPSPFPALTQIEVYG
                     KEA"
     gene            complement(11402..12334)
                     /locus_tag="ACZPCG_06190"
                     /pseudo
     CDS             complement(11402..12334)
                     /locus_tag="ACZPCG_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569632.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphomannose isomerase catalytic
                     subunit"
     gene            12551..13441
                     /locus_tag="ACZPCG_06195"
     CDS             12551..13441
                     /locus_tag="ACZPCG_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1284693.1"
                     /translation="MELLQLRYFLTAAEYQHITKAAEHLQIAQPALSQAIHRLEAELG
                     VPLFERKSRSIELNDAGRFLQKRLIPIISSLDQLAAELQMGREQAIHTIRLRLLSASA
                     LITNRIIAFRSLRPDVNFQLYQTSDQSDDYDLCVSARRADIPSPTGEQPIVMLEEDLY
                     LAVPTHSPYGHRTSIRLTDTKDAGYICLAGSRPIRQITNSYFMEAGFTPRIVFESDTT
                     ESVRNLIAAGMGIGFWPQYSWGPLPSEWNSQSSSPVRLLPIEGQVCRRQITLTRSPKS
                     VDKPVLNDFCSFLVRNSRWI"
     gene            complement(13470..13898)
                     /gene="atpC"
                     /locus_tag="ACZPCG_06200"
     CDS             complement(13470..13898)
                     /gene="atpC"
                     /locus_tag="ACZPCG_06200"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016280314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HIR1284694.1"
                     /translation="MSTFWLKVIASDHTFYNGNCEMLVVPAHDGEFGILPHMEAMILA
                     IREGVLKFRIPGSKEIEEAVVGTGIVQVANNRVTVVTDTAERPEDIDRIRAEQALERA
                     REQLRQKQSIAEYKMSQAALARALNRLQVSQKKGTYYNGL"
     gene            complement(13900..>14802)
                     /gene="atpD"
                     /locus_tag="ACZPCG_06205"
     CDS             complement(13900..>14802)
                     /gene="atpD"
                     /locus_tag="ACZPCG_06205"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HIR1284695.1"
                     /translation="IAMEHGGYSIFTGVGERSREGNDLWTEMGESGVLKKTALVFGQM
                     NEPPGARMRVAETGLTMAEYFRDVQHQDVLLFIDNIFRFVQAGSEVSALLGRMPSAVG
                     YQPTLATDVGALQERIASTKNGSVTSVQAVYVPADDLTDPAPATTFAHLDATTVLSRK
                     IVEQGIYPAVDPLESTSRILEEDVVGKEHYEVANKVQQMLQKYKELQDIIAILGMEEL
                     SEEDKLTVGRARKIQRFLSQPFHVAENFTGVPGKYVPVKETVRGFKMIIDGEMDSYPE
                     AAFFNVGTIDEVIEKARSMEEKER"
BASE COUNT         3897 a         3868 c         2820 g         4217 t
ORIGIN      
        1 gctaagaagc acaacggccg ccaggatcag taaatttttt acagctaagt tttctatatc
       61 tttcatttcc ctgttatctg ctgcctgtct tgtgaatggt aaaggtattg tctattgtct
      121 ctacctttcc attatccgta atctgataac tggtaatctt gaaatcatca taagtaagat
      181 tgatcacaga atagctgggg agccagttct ggcttcttac ctcaatataa tcctgctggg
      241 tatttgtaag ctcataatat ttggatccgg atgcagagtt agccgtcata tacagcgttc
      301 ccttaggatt tgttacaacg gactgaccgg aaccttcgat cgtataacaa tggttatcct
      361 gggcaaaggc ttcctttaag gctgccgacg gctcatcctc cggatacagc gcaatagact
      421 catctgtatt tacattatag gcgtgatccc agtcatagtc actgcctgtt tcatccagac
      481 ggaattcata cgcgccatgc gtttttccat ccccataaag cagcttagaa cggctgtatg
      541 tatggtcatg tccctgcaaa accacatcta catcatattt gtcaaataca ggcgtcagct
      601 gagtacggag gatcataccg tctgtatcgg aatgatccag accggatccg taaatatcct
      661 gatggatcgt aacaatacgc cactttgtat cagggaaatt ctctaccgcc tgtttaatgg
      721 cctgctcatg ttctgccaca ttgtaattat tcgtatttaa aacaataaac aaacccgggc
      781 catatgtata gtaataatcc ccgccggcct cagtcatgcc taaattggta ggattgggat
      841 tattaaaatg atagctgtag tctttattta aagagtcatg gtttccgatg gtagtggcta
      901 ccggaaggct ctttaacgcg cttgcggata ggtatgcagc atactcttct tccttaggct
      961 ttccggtttt attcacctga tcaccagcag aaatcacaaa attaagatca gggttttctg
     1021 ccagggcaat atccagcgta cgatcccagc caaatccatc attacgggcc gcagtgttcg
     1081 ctgcgcctgg atccgctgac agcttaccct ctccctgggg ctgtcccttt gaagcgccca
     1141 cctgcggatc gccgacgtaa agaatattta cattgctgaa gctgcccgtt ttataaacct
     1201 ctgccggagt cctgacgccg tttttttcaa cagaatagta atatgtactg ttctctttca
     1261 gtccgtctac agtcacataa ttgtaagaat aaacttttcc acctgtaagg tttgtatcca
     1321 caccagatgc ctgaccttcc caggccttta agtcatctgc gtctattcca aaatatacca
     1381 ccggagttgc attttcgccc tcgctatacc atgcaaaatt cagctgagac gctgccgctc
     1441 ccggagtaag ggatacctga gtatagtcat ttgccagctg ctcccactgt ctggtccacg
     1501 cctgccattc tgcactccct ccggttacgg atgagtcatt gtaatggaca gtcgcagccc
     1561 acacctgatg accgcctaca gccaacgctc cgaccagaat tccggccata actctcttct
     1621 ttttcataac atttcccttc ttcttttagt tttcaggcct gctattccct gataaatata
     1681 agtatagaaa ggaaatgtaa aatttactat caggtttatg tatgaaatac atcaattttt
     1741 gttactcttt tcctgtttcc ggcaaaaaca gctctccggc tgcttttgca tgactgacaa
     1801 acagatgccg tatcagttca atctctccca gtccttttaa aacacagctg acttcatatc
     1861 cggccctttc aaattccctt ttccaggatc cttcttcgtc tcctgccata tcattgtttg
     1921 catggtctcc tgccacgatc ataagcggct gaagtacaac cttcttatag gatccttttt
     1981 tcacttccct gagtacatct tcaatagacg gctccgcttc tacagtccct atataataat
     2041 tcgtaaaccc cttttgcagg agaatactct gaagcttttt atatacctga ttagcttctg
     2101 cctctgtacc gtgtcccata aagcaaacag ctgtctcacc atcatcatat tcctttgtat
     2161 cttcggtaat agcgcggatc actgtttcaa aatcttcctc tgatgtcaaa agcggctctc
     2221 ccacagccat gtattcaaat aatgccctgt actccgatgc ttcttcagcc agatcctggt
     2281 attcaaatcc cttcatcata tgtgtaggct gaatgatcag tgtttttaca ccattctcta
     2341 ccgcatgctt taaagcatct cttacattat caataaccag attttctttt tgttttaccc
     2401 ggttaataat aatctgactt gtaaaagccc gatgcaggat atattccggc atagcgctct
     2461 ccatatctct ttctatggct tcaatggttc tttctcttgt gtttttgtag ctggttccaa
     2521 aactgactgc cagcagttcc cgttctcctg ttttattctg atttgtaccc ttgatttccg
     2581 atactgtttt caaattcatt cgttcattct cctgttgtgt ttttaatgag tatttacagt
     2641 ttaatggaaa tcattttttc tgtcaatttt tttacagccg atgcagctcc tgcatctcgc
     2701 caaaggcact tcttcccata cagtctgaaa gttctcttct gatcgactca aaatcctcat
     2761 aaaccaattt tttatcctga accaggcagg tacctgccac atatacatct ctcacattgg
     2821 atgcattggc actgtaaacc agggcagaat agggatcgta aacaggaaac atattgggag
     2881 aatctgtttc caccagtacc aggtctgctt ctttgccagg ttcaatagaa cctgtttttt
     2941 cttccagtcc cagaaccctg gctccctgac gggtcgccat ggaaacaata tcttttgcag
     3001 gcatggcact tctgtcatgg ctgtcgtttt tctgaaatcc ggcgcacaaa cgcatctgcg
     3061 taaagagatc cagagtattg ccgctggccg gtccatctgt tccaaggcct accgaaacac
     3121 cttcctttaa cagcttaagc accggtgcta ctcccttcgc cgccttggca ttggagccga
     3181 tacagtgaca cactccgctt cctctctgcc ttaaaatttc tatgtcacga tccgtcagat
     3241 ggatgcagtg agctgcagcc gtttgatctg aaaggacccc cagctgatcc aggtatccaa
     3301 ccggcgtaca gccccgtgtt tgtgccagcc aggtcatctc gtaatccatt tctgctgcat
     3361 gaagagtaaa tggtacctga ttggcagtat caagttccca ggcttttttc agaatctgcg
     3421 gactgcaggt attagtagca tgaggtgcag cacatccact gatacgtgga tgcattctgt
     3481 atgtttctat cagttctttt gccatactca gagactgatc tgcatcagaa gcgtcacatc
     3541 cgccttcatc cattaatgtc tgtccggcaa ttcccctgat tcctgtctga tccataacct
     3601 gagctacagc agcctcaaaa taatacatat ccattacgga agtaactccg gacagcagca
     3661 gctcgcaaat tgcatagcgg ctggaaagtg ctgccattct ccggctcata gccttctgct
     3721 ccattggaag cagaaacaca cgaagccggt ctttacagtc atcgcccagt ccccgaaatg
     3781 gaatcattcc cagatggcag tgtgtattaa ccataccagg cataagaatt cctctttttc
     3841 cgtcttttat ctgccagcca gcagctctta attcttctgt tgtctccttc agatcacagg
     3901 aaggccctgt ctccagaatc ttcgtacctt ctattttcac atagccgtca ggaaacacct
     3961 ccatatcaga attcattgta atgataatag agttagtgat tatggttttc atctttatac
     4021 ctcgataagc tctacttgtt tctgggtttt tacatccagc aatcctctgt ctgtaagctt
     4081 taatgccggg gaaacaggaa gcgccagagt agacatggac atgatttcat tattattttt
     4141 atatcccaga gaagccatgc tctttcttat ctgtcccagt tcacaggaaa gtttttcaag
     4201 agaaccttca tttataattc ctgccacatt cagctgtacc tgccccagac aacagccgtc
     4261 tgaaaaaata cagtatcctc cctcaagctc taaaatacgt ttctgagcca aaaccatatc
     4321 ctcaggagaa gttcctaaaa ccagcagatt atgactgtca tgacaccagg ttgtagctgc
     4381 agctcccttc tttgcaaatg ctccttccac aagtccgtaa cctatatttc catttttccc
     4441 gtggcgctca tagagaactg ccagactcag ccctgcttcc tgccagcaga caattccatc
     4501 ctttaccgga agctgacgct ttatgtgtcg ggttgctgtt ccgaatgtat taatctgcat
     4561 cacattggct gtaacatgcc cgaatttcac cggtgctttc aggatgaaat cctccagtac
     4621 agccggcctg cagtggacgg aatgataaaa atgctctgga aagttcgttt cttcttccat
     4681 accgcagcgg gaatctccct caatccaggc aaggcgtcct tctttcacca ctgcttccgg
     4741 ttccatggta tccagatctt taaggaacac aaaatcagcc agttttcccg gaccaatcat
     4801 tcctctgtca tccagatgca ttcttctggc cggtgtataa gtggcacagt aaattgcctt
     4861 ttccaccggc attcctgcca gaacagctgt ccgcacaatc tgattcagat gccccttcaa
     4921 aagctgatcc ggcataacat catccgttac aagtgcagta ttttcgtaca gattataatg
     4981 gttcagcgtt tctaccacct cgggcgtcag ggattttctc tgcagttcca gaaacattcc
     5041 aagatctgtc ttttccagaa cagactcggg agtctgctga gtatgatccg catcaattcc
     5101 ttcataaata aaccggttta agtcagcacc ggaaattctg gggcaatgtc cctccaaacg
     5161 gatatccctt cctcttttct tccgacacag acgaatcatc tgccgaatcg gcgtatctct
     5221 gtcatcttca ggagccagct gcttaaaatt catcacttct cccagacaaa ggatatttct
     5281 gtcctccaaa agagctgctg catcctcagg tgtaatagtg gctccagatg tttccaaagc
     5341 tgtatctgta gccggaacgc tggaaggaat agcccagaaa atatccattt ctgttttctg
     5401 agattgaaat tccgcaatcc ctttcatgcc gaacacattg gcaatctcat gtgcatctgc
     5461 cactaccgtt gtagtcccat gagacagagc cagcggtccg aaacgggacg gacaggtcat
     5521 ggagctttct atatgcatat gaatatcaat cagccccggg atcatataac gcccctgcgc
     5581 atcatagacg ggaacgcctt cgggataaac acccgaatta acaggaagga cggcataaaa
     5641 acggccgtcc tttacagctg catcccggag ttcaaaacac tgacggaaag tctggtagat
     5701 attgacattt cttataatat aatccgggga catagttcct gctcctttag ttattgataa
     5761 ttctgttcca ctgatcgatc cactgattta aaagaggatt aacaaagcca tagtccagaa
     5821 cttttgcttt ttctgctgcc ggaccgtatg tcatattttg ggactcctcc tctgtaaacg
     5881 taactgtttt attcgttgga gcctcattta aggcttttcc tgtcttagac tgaagctctg
     5941 cacttaaacg gtaatttaaa tactcataag ccagttcttt attctggcaa ttcttcgtaa
     6001 tactgatggt gttaaaattc gcataggttc cttccggagt tacgtataca agatcaggat
     6061 ttgctgcctt aatcgtcgga acaccaaagt ctcccactac agcagctgct acttcaccgg
     6121 acgtaaacat gttgatcagg tctgaggatt tggcatatgt ttttaccaga tttggcttta
     6181 attcctccag tgccttaaaa gcagcctctc cgttatcggt tttaatatca actcctgcat
     6241 gatcactggc catatatacc atagccggtc cgaaggttgt agtaatctcg ggaatagata
     6301 ccatacctgc cagctccggc ttccacagat catcaaagcc tgtaatctca aaccctactg
     6361 cttccggatt atacataatc ccaatactgt taatagtata aggaacaccc tgaccattgt
     6421 cagccagcgt ctttgctgcc gcgatcagat cagaggcatg ttcaatttta cttaaatcaa
     6481 tggtctcaaa caaaccttcc tctgcaccct tggcagccat agcctgagac agttcaatta
     6541 catcaatcgt agtttcactg tctgccttca gcttagtata tctgtcattg gttcctccgg
     6601 tttctgtcac aatctggcag ttaaactgct cttcaaaagg agcataaacc tcctctgctg
     6661 aaatatcctc actcaaacca tacgtagaca gaaccagttt ctgctttcca cctgcctgtg
     6721 caccttctcc ttctgatcca ccggaacagg ctgttactgc catagctgcc atgcagactg
     6781 ctgctaatac tctctttttc atcttttttc tccttttata ataatttcaa ttttctgctt
     6841 tttaccgccg taccaggatc agcttatcct caggaagata cagacgaatc tgatccccct
     6901 cagcatagat acattccgta ttcatgttaa ccttcagaac accagcagct gtttccactt
     6961 catactgata gctctttcca agaaaggtgc gaacgccgat tcttccggta ataacatttt
     7021 cctgctgatc cggtgctgca atacggatat catccggtct gatcgttcct gtaaattctc
     7081 cttccggaca ttccataaaa gaacagaaac ggctctgacc gcctgcaaga taacttccat
     7141 ccccacatct ttccagattc aggaaatttt caaatccaat aaaccgggct acaaactctg
     7201 tggcaggctg tctgtaaatc tgttccggtg tgtcatactg ttcgatgatc cctccattca
     7261 taacagccac tttatcagaa atagagaaac attcttcctg atcgtgagta acaaacaaag
     7321 tagtaattcc cagttttttc tgaaggcgct taatttcgac tctcatactg atgcgaagtt
     7381 ttgcatcaag attactcagg ggctcatcta aaagaagaag cttaggttcg attaccagtg
     7441 ctctcgcaag agcaactctc tgtctttgtc caccggacat ttctctcgga aaacggccag
     7501 acagctctgt caggccgcaa acctccagaa tctcttttac ctttctgtca atctgtgcct
     7561 tatccatctt gcgcgtacgc agtccaaaag ccacattatc atataccgtc agatgcggaa
     7621 aaagggcata gctttgaaat actattccaa aatttcgctt gtgtaccgga acctttgtca
     7681 tatcttctcc gtccagtgta aaggttcctt cacctggttc aataaagccg gccactaccc
     7741 gaagagtcgt agtctttccg catccgctgg gaccaaggag tgataccagc tcgccttttt
     7801 ccacagacag atccaggccc tttaagatct gttttttctt atcatagctg acgccgattt
     7861 tctctaatgt cataaatgcc attttcttat cctcctattt tgccagtgaa gcaattccca
     7921 gggttctgtc cacgattacc ataataagta cagtagctgc cattaaaagc acagaaacag
     7981 cggacaccgt agggtcataa ttgtattcaa tataattcat cagtgttgcc gggaaggtgc
     8041 tgacaccggg acccgttaaa aacatggata caggaatatt attaaatgag ttgataaaag
     8101 ccagcataaa agcagatgaa ataccggagg ttacattggg gagaaccact ttaaaaaatg
     8161 ctgctccctt aggacatccc agactccagg cagcttcttc aatagaaaaa tcaaactgct
     8221 ccatagcaga accaactaca cggattacat aaggcagagt tacaagaaaa tggcctgcca
     8281 gaagacttgc cataaccgga aggcggaaag tcagaatcag gaactgatac aggataaaac
     8341 ctactacaat cccaggaaca atggttggag acaaaaatac ggatcgcaga agctgttttc
     8401 cgcggattcc tgaccgtgcc aatgcgtatg ctgcaggaat tcccaccaaa agagcaataa
     8461 aggttgcaag gaatgccact tccagactgg tcagaaagga acgccggaaa gacttcaaag
     8521 caaaaacatt tccaaaccat tttagggaaa aagaatcaat tggaaatgta atcgcacttc
     8581 ctcctccaaa agccgtaacg gtaataatca ccagagggat catcaaaaac aaaaatacca
     8641 ggacagcgta aactgtcagc caacggttct ttttcattat tttcctcctc ttttatccaa
     8701 tcttgctgcc agagcattaa agcccctgat tactataaga gtaattacga tcataatcag
     8761 cgctactgca gaagccccgt tccaatcccc tacagacatg gctctctgat aaatcagagt
     8821 tgccagaacc atattgctgt ttcctccaag cagctgaggt gttgtatatg cagtgagggt
     8881 tcctgtgaaa acaaggatac ctcctactat aattcccgga agactcatag ggaaaatcac
     8941 tttcataaaa gcctcaaatc gtcctgctcc cagactctga gccgcctcca tcatatcatc
     9001 atctatattc tccattacac ctgtaaccgt aacgatcata agaggaagaa acagatacac
     9061 agaaccgata ataatagcaa aatctgtata aagaagcttc atgggcttat cagctacatt
     9121 gagagccagc agaatgcggt tgattatacc gttgcttcct aaaatattga tccaggcaaa
     9181 acttcggatc acagaatttg tcatcattgg aaaaatggat gctgccagaa gaagacctct
     9241 ccatttttta tcacaccggc ttataaaata tgcggtggga atccctcctg ccatgcagat
     9301 ccctgtggta ataactgcaa ttttcagggt gcgaaacaga atttttctgt agtactcatc
     9361 ctgaaaaaac tccacatacg cactgagagg atattgtcct gctgtaaatg taggggccag
     9421 cacacttata aggggcagac ccagacacaa cagcaaaatg atcagcccgg gagccagcat
     9481 catccatgta ctgcttttct tcatgttctt cctccattct gaatatattc attaattata
     9541 ctaattttta attctgaatt tattataaaa agtaatatac attccgtcca atacataatt
     9601 tctatatttt ttatgcagta aagctatgat atactgatta aaaaggaggt taatccatgg
     9661 atttaaaaga agcacgctac attctggcta ttgcacacta taaaagcatc ggaaaagcag
     9721 caaaagccct gtttatctcc cagccttctc tcagcaaata cctcaagaat ctggaacagc
     9781 agcttggctc ccccctcttt tcccgtctgg aacagggata catccctact tatatgggag
     9841 aacgctatct atactatgcc gaacagatcg cagcctttgg ccaggaatgg gatcaggaat
     9901 atgaagatat tacccgaaga gcccatggac ggctcaatat tgccattccc atcatgctcg
     9961 gaagcacttt actgcagcct actctgatgg aattccaccg gcgctatcct catgtgacaa
    10021 ttaacattat ggaggaagtc agctttgtcg ctgaacagac gctgaaaaat acctctattg
    10081 accttacaat ttataatgta catacttttc caaaattatt agattatcag gttatccgca
    10141 cagaggaaat cgtactgata ctgccggaag gccatcctct tgaaaaagag gccgaaaaaa
    10201 aagagggctt ttctcatcca tggctggatt taaaaaagct ctctcaggaa aattttattc
    10261 ttttatatcc ggatcagaac accggcggca tcgccaggga tctcttcgcc cggcatgaaa
    10321 ttaatcctcc ggtacttctg catacacgca acagccagat gtccatcaaa cttgctatgg
    10381 acggaatggg agctgccttt gcaccagaaa gttatttcaa ctatatggca tctcttcgtt
    10441 cagatcggcc aggctgctat tgtgtaggag aaaaacctgt ccttaccaca accattgcag
    10501 cctgccggcc cggaaggtat atgccaaaac atgttaaaga ttatatagat ctgttaaaaa
    10561 tatatggaaa cagctcttcc taagcttctt ttccataaac ctcaatctgg gttaaagccg
    10621 ggaatggaga tggatcgtct gccttgatca gatcagacag gcgtacccag gtaatcccct
    10681 tctttgtaat ggcaaaggta tgagccttct cgctcttttc catatttacc acctctctgg
    10741 tgccgtctga aaaggtaaga actgcgctga cccaccagtt atcatgcgga aaatcagctc
    10801 tggtccacaa acgcacctga tcgatgtcca ccggacggcc aaactccagc aaaagttccg
    10861 catcatctct gcggttgatg ccccaggact catatggcca ttttccgtga ctcagagtag
    10921 cggttacgcc atcaattgca ttacgggctg caaaaacaga ctcccctctt gtttccacat
    10981 ttgcagaagc atgaggatag cagcctctgt ccccgtgctg atcaagcaca tttttagcca
    11041 gattacgata aataccggtt tcccatgaag cagccttacg gcaggtcaga taatgacgca
    11101 caccagtaaa actcttggga ttgtaggaga atttcttttc atcaaacggt acctcaaacg
    11161 taagatcctt ctttgtcata tataccagag cttcatccat ggtatcatct acacgtacca
    11221 tataataccc ttctgtttca ggaaaatgaa actgaatgat atctcccggc tcatatacat
    11281 ccttaaatgc cagtatgctt tcctccggat cctgtgcaaa tgccttggta tgacccttcc
    11341 agtctgaaac acggatagaa atctgatcct cttttgtctc ctttgcctct gccgggatcc
    11401 aggagcagat aagagtcata tttcccttaa attcacagac attatgacct gccgggacaa
    11461 taaagtgcgt tcccttcttt accggaattc catctaccgt tccttctcct tccagaacgc
    11521 tcacattggc aaaacgatct gaaaacttat gctgccagca gccatgaacc tcgaatttct
    11581 ctacggtata aaatgggcag gtttccagat gctcctgatc cgccgcctct gtatggtacg
    11641 attttctctg atcctttgca ggtacaaagg gggtcttgat cacgtccagg ctttccttta
    11701 catggagcat tctgggcgct ccgttccact ttctgtcata atcgtacaca cgatacgtta
    11761 catcactgga ctgctgcgtc tccagaatca cagtaccgcc tttgatagca tgaacacagc
    11821 ccggatcgat ctggaagaaa tctcccttat gaaccgggat ctgacggatc agctgagaat
    11881 aatttccctg ctccaccatc tgcttcagct cttccttatc ttttgcatgg tgccctataa
    11941 caatagaagt atccggctca caatccagaa tgtaccagca ttctgtcttt cccagagaac
    12001 cattttcatt ggctgcggca tatgcatcat ccggatgaac ctggatgctt aaatcatcct
    12061 ttgcatcaat aatctttata agaagaggga agcgatctcc cagacaccca tcctcattgc
    12121 caaataattc cggatgctct gcccataaga cagacaaacg ctgtccctta tattctccct
    12181 ctgccacttc acagtctcca ttttcatgag cactgatagc ccagcactca ccggtatggt
    12241 cgcttgggat ttcatagcca aactgatcct tcagccggct tccgccccat acggcctctt
    12301 tgaaaatcgg tttcaaaaat agcagttcgt acattttaca cacacctttc tatctttact
    12361 gtatctgatc ctgtttcaaa acagagacag cactcatgtt catcttagcc gtacctggct
    12421 tatccgtcaa gcgttttcct tttaaaaggt gagctaaact gtaaaacaca tacaaaaaga
    12481 agcagtccgt ccattgacat atctgcttct tcatgctata tttcataaat atttttttga
    12541 ggtgatacat atggaacttc ttcaattgcg atattttctc acagcagccg aataccagca
    12601 tataacaaag gctgctgaac atcttcagat tgcccagccc gctctttctc aggcaatcca
    12661 ccgtctggaa gcggaacttg gcgttcctct gtttgaacgc aaaagccgct ctattgaatt
    12721 aaatgacgcc gggcgcttcc tgcaaaaacg gctgattcct attataagca gcctggacca
    12781 gctggcagca gagctgcaga tgggaagaga acaggctatt cataccatac ggcttcgtct
    12841 tttatctgcg tctgctttaa tcaccaacag gattattgcc tttcgcagtc tccgtccgga
    12901 tgtgaatttc cagctgtatc agacttctga tcagtctgat gactatgatc tctgtgtttc
    12961 cgcccgacgg gcagatattc cttcgcccac aggcgaacag cccattgtaa tgctggaaga
    13021 agacctttac cttgcagttc ccacccattc tccatatggt caccgcactt ccatacggct
    13081 gacagataca aaagatgccg gatatatctg tctggcagga tcaagaccca tccgccagat
    13141 taccaacagt tattttatgg aggctggctt tacacccaga attgtttttg aaagtgacac
    13201 aactgagtct gtacgcaatc tgattgccgc cggaatggga atcggcttct ggccccagta
    13261 ttcatgggga cctctcccct ccgaatggaa ttcccagagc agttcccccg tcaggcttct
    13321 ccctatagaa ggacaggtgt gccgccgtca gatcacgctt acccgatccc ctaaaagtgt
    13381 ggataaacct gtattaaacg atttctgcag ctttctggtc cgcaattccc gctggatctg
    13441 agcgttgtgc agaccgctta taaatcttcc tacaggccgt tataataagt tccctttttc
    13501 tggctaacct gcagcctgtt cagagcccgc gccagggccg cctgagacat tttgtattcc
    13561 gcaatgctct gtttctggcg cagctgctcc ctggctcttt ccaaagcctg ctcagccctg
    13621 atccggtcaa tatcctccgg ccgctctgct gtgtcagtaa caacagtgac tctgttgttc
    13681 gctacctgaa caatccctgt tcccactaca gcctcttcta tctccttgga tccaggaatg
    13741 cggaatttca atacgccttc ccggatcgcc aggatcattg cctccatatg tggaagaatc
    13801 ccaaattccc catcatgggc aggaacgacc agcatttcac agtttccatt ataaaacgta
    13861 tggtcacttg cgatgacctt aagccagaat gttgacatat taccgctcct tttcctccat
    13921 ggacctggct ttttcgatca cttcatcaat ggttcccaca ttaaagaagg ctgcttccgg
    13981 ataagaatcc atttctccgt caatgatcat cttaaagccc cgaaccgttt ccttaacagg
    14041 tacatatttt cccggaacac cggtaaaatt ctcagctaca tggaagggct gagacaaaaa
    14101 tctctgtatt tttctggcgc gccctaccgt aagcttatcc tcctcagaca actcctccat
    14161 accgaggatg gcgataatgt cctgcagctc cttgtatttc tgaagcatct gctgcacctt
    14221 atttgccacc tcataatgct ctttacctac tacatcctct tccagaatac gcgatgtgga
    14281 ttccagagga tccactgccg gataaattcc ctgttctaca atttttctgg aaaggactgt
    14341 ggttgcatcc aggtgggcaa aggttgtggc gggcgccgga tctgtcaaat catctgcagg
    14401 cacataaact gcctgtaccg aggtaacaga tccgtttttt gtagatgcga tccgctcctg
    14461 aagtgcgcct acatcggttg ccagcgtcgg ctgatatcct acagcagagg gcatacgtcc
    14521 caaaagagcc gagacctcag agcctgcctg aacaaaacgg aaaatattat caataaacag
    14581 cagcacatcc tgatgctgta catcgcggaa gtattctgcc atggtaagcc ctgtttccgc
    14641 tactctcata cgggcccccg gcggctcgtt catctgtcca aataccagcg ctgttttttt
    14701 tagcactccg gattctccca tttctgtcca aagatcattt ccctctctgg aacgttcacc
    14761 gactccggta aaaatggagt atccgccatg ttccattgct at
//
LOCUS       DBMDUH010000053        14732 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_2230_length_14732_cov_4.762354, whole genome shotgun sequence.
ACCESSION   DBMDUH010000053 DBMDUH010000000
VERSION     DBMDUH010000053.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 14732)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14732)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14732
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2230_length_14732_cov_4.762354"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(436..1950)
                     /locus_tag="ACZPCG_06210"
     CDS             complement(436..1950)
                     /locus_tag="ACZPCG_06210"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003523497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HIR1284696.1"
                     /translation="MQQYQNPILYADYSDPDVIRVGEDYYMVSSSFTYLPGVPLLHSK
                     DLVHWELINHCVSSLPFEKYDFPSHGSGTWASSIRYHEGTFFVFIPLVDEGILVARSR
                     DPYGEFELNLLCKSKGWIDPCPFWDEDGKAYMVFAYAKSRCGMKHRLSMVEIDPNCRG
                     LLSEPKLIFDGEQIAPTTEGPKMYKKNGYYYILMPSGGVEDGWQSCLRSRSVYGPYEY
                     KIVMHQGNSAVNGPHQGGWVTSSDGRDWFIHFQDVIELGRIVHLQPMCFADGWPFIGQ
                     DQNGDGIGEPVESWYMPEEDMPSYAIAQSDDFEEKHLGLQWQWQANPNSAYYSLTDRP
                     GYLRLFCWKNPERENYLWYAPNVLTQIPQKKELTMTVKMELTGKEAGDFGGIGVEGHI
                     YGYAGLYSTGEKMEIRCYRGIVTEKMFHGEAEERLVFSSKLEGNVLWLSMSLSDDKTY
                     KFSYSTDGVHFTQIQEKFPLSRATWTGAKLCLWSCSKENKNSEGYCDYEYVTIV"
     gene            complement(1966..2805)
                     /locus_tag="ACZPCG_06215"
     CDS             complement(1966..2805)
                     /locus_tag="ACZPCG_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1284697.1"
                     /translation="MREKRIKNGFTYTFLAIGGVAMLFPVIWMFFACFKTNNEIFGSL
                     RLLPESWVIEAFVKGWKTTGTYTYAQYFINTFALVVPTTFLTLVSCSIVAYGFARFDF
                     PGNKALFMVLIATLMLPNAVIIIPRYALFNKLGWLDSYMTFYAPAAVACYPFFVFMMV
                     QFLRGLPRDLDESAYIDGCGPFQCFMKILLPLLKPALFSAGLFQFLWTWNDFFNTNIY
                     INTVSKFPLSLALRVSIDVTSNIQWNQVMAMALVSVIPLIILFFAAQKYFVEGIATSG
                     LKG"
     gene            complement(2815..3726)
                     /locus_tag="ACZPCG_06220"
     CDS             complement(2815..3726)
                     /locus_tag="ACZPCG_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1284698.1"
                     /translation="MAKKGAKSGSMHGGWTKRNKIGFMYVSIWIFGFLLFQLYPFICS
                     LVYSFTDYDIFSKPEFVGFANYIKLFTKDKEFWNSLIVTLKYTFITVPGKVVLALIIA
                     VILNKNLKGINFIRTVYYIPSLLSGSVAVAILWKVLFMNDGFVNSLLGCFNIEPVKWL
                     GTPDMALVTICMLEIWQFGSSMVLFLSALKQVPQSLYEAARIDGASKPRMFFKITLPM
                     ITSIAFFNIIMQLITALQNFTSAFVVTNGGPNKGTYVLGMKLYTDAFKYFKMGYACAT
                     SWILFIVILIMTAVLFATSKKWVYYDN"
     gene            complement(3820..5178)
                     /locus_tag="ACZPCG_06225"
     CDS             complement(3820..5178)
                     /locus_tag="ACZPCG_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002605201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1284699.1"
                     /translation="MKHGKKLTGLVMAGIMTAGVLTGCTNEPSANTASDTKTEDQSAK
                     SETASQEQITLRFMWWGGDERNEATLAVIDQYQKLHPEIKIEAEMNSDQGYIDKVSTM
                     LANGTAPDIMQQNVDSLPDFVSRGDFFVDFYEYKDIFDTSGFDKNFIDQFGTFDGKLL
                     ALPTGMSCLATVANTEAADVCGVDLSKQITWESMLEDGKKLHAENPEYYYMNVDTHIL
                     CEYVLRPYLRQKTGNSFIIDSEKKLGFTRDELVEALTYIKDCYDWGVFEPAEDSATFK
                     GQIHTNPKWMNGKFVFGYGASSNINLLMDAISEAECTVVQMPMADDRINDGFFADTPQ
                     YMTVNKNSPHAKEAVEFLNYFYNNETAQETLKDVRSIPPTTTGRSLCAGKGLLDETVV
                     KAVDLSAGLNGKSDKGYTTSAEVYAIQEEIVESIAYGQSTPEEAADNAIELLNDYLSA
                     LD"
     gene            5503..6258
                     /locus_tag="ACZPCG_06230"
     CDS             5503..6258
                     /locus_tag="ACZPCG_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003523493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1284700.1"
                     /translation="MYQVIIADDEAKIRSGLANLFPWNQLGFEIAAVCSNGKEALDFA
                     MENPVDLVLSDIRMPIMDGLELSRLLLPKKPVKMVFFSGYQDFNYARQAMKNGVSDYL
                     LKPVKYEDLSDCLTRIRETLDMEKGLAQPEEENLSYYEKIINTVTSYLDQEYQTASLE
                     QAARLVSLSPNYLSRLMKDHSALSFSDYLLKSRMEHAANMLKDIHFKQYEIAYRVGYD
                     NPKNFSRAFHQYFKMTPTQYRNCSGVTEKRNEK"
     gene            6274..8010
                     /locus_tag="ACZPCG_06235"
     CDS             6274..8010
                     /locus_tag="ACZPCG_06235"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1284701.1"
                     /translation="MRKRNFINCFFYYFAIITLPLLMVFLFFLFFVIQEKKQEIQSNA
                     KRSAQAAKDNYSLVMDTAATQYDILARNPRLSISLRNFLSHEKANYLDVVLTNSILSN
                     FTSLTNNAPYIASVYYYLDDYDSILTSDTGGIVKLSRFSDLSWKDSYDSSDKDQWLER
                     RQMHQYSYAEPIPVLTYFKKLNMFRGVVMVNIYEEQLKQLITTSSQLDSFFILDQNGN
                     FLLEAGKTKQFTHEDRGLLANKLSSVSLSPFEGWFPFSNGSCYTRMIPADCDTYIAAC
                     ISYSNYYRDFYPLLLQLLMVLAAMVFVSLWIAFTITRKNFQQIDYFVNLFSDAERGVF
                     RQEKPDFLRDEYDLVLNNIVQLFLNQTFLKNQLALKDQKQKLAEMKALQLQINPHFLF
                     NTLQTLDFKALEYTGQPTAMNQMIQALSDILKYSLQNPHSMVTLKDEIDYLKKYDSIQ
                     RCRYEDKYILYYEYEETLENLPIMRLILQPLIENSLYHGIKPLDDSGFIKLRIVRRKN
                     HLIVSVIDTGVGMDRQAITALYKRIDNPDSDNIGLTNVNSRLVMKYGPEAHLKINSKK
                     GMGTCISFRIPI"
     gene            complement(8130..9599)
                     /gene="xylB"
                     /locus_tag="ACZPCG_06240"
     CDS             complement(8130..9599)
                     /gene="xylB"
                     /locus_tag="ACZPCG_06240"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004078188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HIR1284702.1"
                     /translation="MLYIGIDLGTSAVKLLLMDQGGTIHKIVSKEYPLYFPHPGWSEQ
                     NPQDWFEKTMEGMKELTAECDRTQIAGIGFGGQMHGLVVLDEMDQVIRPAILWNDGRT
                     AKETEYLNEVIGKEALSKYTANIAFAGFTAPKLLWMKANEPENFARISKIMLPKDYLA
                     YMLTGIHCTDYSDASGMLLLDVEHKCWSREMMDICGVKEEQLPQLFESYEVVGLLKKE
                     IADRLGLTEAVRVVAGAGDNAAAAVGTGTVGEGKCNISLGTSGTIFISSKKFGVDEHN
                     ALHSFAHADGSFHLMGCMLSAASCNKWWMDEILKTTEYEREQDSITKLGENHVYYLPY
                     LMGERSPHNDPKARAAFIGMTMDTAREDMTQAVLEGVAFGLRDSLEVARSLGIQIERT
                     KICGGGAKSLLWKKIIANVMNLKVDVIESEEGPGYGGAMLAAVGCGEFETVEEAAEKL
                     VKIVDTVEPETGLVEKYEKRYQVFKQIYPAMKGLFQLGM"
     gene            complement(9612..11096)
                     /locus_tag="ACZPCG_06245"
     CDS             complement(9612..11096)
                     /locus_tag="ACZPCG_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009266987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose/L-arabinose isomerase family protein"
                     /protein_id="HIR1284703.1"
                     /translation="MKNMPELKIGVVAVSRDCFPESLSVSRREALIKAYREKYGDRDI
                     YECPICIVESEIHMVQALEDIKAAGCNALVVYLGNFGPEISETLLAKHFDGPKMFVAA
                     AEERGDNLIQGRGDAYCGMLNASYNLKLRNIKAYIPEYPIGTAEECADMIAEFRPVAR
                     AVIALNDLKIISFGPRPLNFLACNAPIQQLYNLGVEIEENSELDLFEAFNKHAGDERI
                     PAVVKEMEEELGAGNKKPEILEKLAQYELTLKDWIEEHRGYRKYVAIAGKCWPAFQTQ
                     FGFVPCYVNSRLTAQGIPVSCEVDIYGALSELIGTVVSEDAVTLLDINNNVPADMYKE
                     EIEGKFPYTHQDTFMGFHCGNTPSEKLSFCEMKYQLIMARSLPEEVTQGTLEGDIAPG
                     NITFFRLQSTADCKLRAYIAQGEVLPVATRSFGSIGVFAIPEMGRFYRHVLIEKNFPH
                     HGAVAFGNHGKALFEVFKYIGVPVEEIGYNQPAGVRYPTENPWK"
     gene            complement(11122..11739)
                     /locus_tag="ACZPCG_06250"
     CDS             complement(11122..11739)
                     /locus_tag="ACZPCG_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009266988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4867 family protein"
                     /protein_id="HIR1284704.1"
                     /translation="MQIQNINEESFKKYGKILTGYHVDGILAEMEHTPLTREVVYVPS
                     VEEMEALPDAEEMKNRGFGGLPIQIGYCNGDNRKLNGLEYHRSSEINIAVNNLVLLLG
                     REQDIEADGTYDTSRVEAFFVPAGTVIEVYATTLHYAPCTAEGENGFRCVVVLPKGTN
                     TELDFAAGAEGEDRLLAAKNKWLISHEEAGIVGAFCGLKGENITV"
     gene            complement(11848..12696)
                     /locus_tag="ACZPCG_06255"
     CDS             complement(11848..12696)
                     /locus_tag="ACZPCG_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIR1284705.1"
                     /translation="MQSNHEIVVPNEDLPFKMFLFEGEKGNYFRDKHWHRSIEIFMVF
                     EGHLKFYLNDLEQELNPGEFVLVNSNEIHAIDSPEPNKTVVIQIPLKTFSDYFTGEQY
                     IRFTHEAKKQDVQVVDLIREMFAAYGKKEAGYDMRVKSGYYMLLYMLVSVYRELDVKD
                     ELLKQNRKLNKLTPITNYMKEHYQEELSLEALAEVFGYTPAYISRMFQKYAGINYKDY
                     LQGVRVEYAFTELSRTEHTVSEVAFHHGFPSSRAFAKAFQKKYGMLPSEYLRRKTESS
                     HSSEQI"
     gene            complement(12860..14053)
                     /locus_tag="ACZPCG_06260"
     CDS             complement(12860..14053)
                     /locus_tag="ACZPCG_06260"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - metabolic compound salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HIR1284706.1"
                     /translation="MRKFKRIFLIVMDSFGAGNAHDAKAYGDEGADTMGHIAEYVGQW
                     NIPNLQKLGLANLHPLKGVEPAVKPLGYYALLNEKSRGKDTMTGHWEMMGIQTAKPFV
                     TFSETGFPEELVKELEQRTGHKVIGNKNSSGTEILDELAEEEIATGHMIVYTSADSVL
                     QICGNEETFDLEELYRCCEIAREITLKDEWKVGRVIARPYVGKKRGEFKRTSNRKDYA
                     LKPSGKTALDALKENGLSVIAVGKIHDIFDGEGITQTYHSDSSVHGMQQTADIMEHSE
                     FEGLCFVNLVDFDAVWGHRRNPEGYAKEVEQFDEKLGELLELMGDEDLLMITADHGND
                     PTYQGTDHTREQVPLLLYAKDLKGAGTLNEADSFAAIGATIAENFNVRMPDGTIGESM
                     LDQIQ"
     gene            complement(14079..>14732)
                     /locus_tag="ACZPCG_06265"
     CDS             complement(14079..>14732)
                     /locus_tag="ACZPCG_06265"
                     /inference="COORDINATES: protein motif:HMM:NF013355.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster-binding protein"
                     /protein_id="HIR1284707.1"
                     /translation="MALYAAEAGADAVTGINSIHGIGPLNPERMVHIPDIMGQSAPMG
                     LSGAFIKPMAQHCIYKMALAAKEKPFGISAVGGITDGKDALEYLCLGADTLQSATAVM
                     YKGYGIIRQMISYLEKFMMEKGYQSLDEFRGYSLNHMASAARNLSLKQQLAALVDPDK
                     CIGCGKCAVSCSDGGKQAIQLETSPRKALVCTEKCTGCGLCQIVCPTGAIQMVPTER"
BASE COUNT         4114 a         3720 c         2555 g         4343 t
ORIGIN      
        1 cgagaggtcg caggttcaaa tcctgttact ccgatttttc ctttaaaatc aagggttttc
       61 gatgattttt gtcatcgaaa gctcttattt ttttgattac cctttcgcta attttcattt
      121 gcctgcatct attttaaata tatcttacca ttccctataa aagattgcaa tatttcaata
      181 ttctggtttt tcatattggt ttaaatccct gttttcataa atacaacgct taccatttct
      241 cagccagact ctgcatcaaa gaaaatgctg caaaaaaaca tgagtactac aaaatactaa
      301 aaaactgcag cttcggttat aacgttgttc ctgataaggt aacattataa ccgaagctgc
      361 tcaaataaaa ccaatcaatg aaaaccgccg caaaacacag caaactttca gtttccagtt
      421 tttgaaattc atatatcaga caatcgtcac atactcataa tcacaatatc cctctgagtt
      481 cttattttct ttggaacagg accacaggca aagctttgcc cccgtccagg tagcccggct
      541 cagcggaaat ttctcttgaa tctgtgtaaa atgcacacca tccgtggaat acgaaaattt
      601 gtaggtttta tcgtctgata aactcatgga aagccataat acatttcctt ccagtttact
      661 ggagaacaca agcctctctt ctgcctctcc atggaacatt ttttctgtaa caattcccct
      721 ataacagcga atctccatct tctctcctgt gctgtagagt ccggcatatc catagatatg
      781 tccttccact cctatcccac cgaagtctcc cgcttctttc cctgtaagct ccatcttaac
      841 tgtcatggtc agctcttttt tctgtgggat ctgagtcaac acattgggcg cgtaccacaa
      901 ataattctcc cgctccggat ttttccagca aaaaagcctt aaataacctg gtcggtccgt
      961 cagtgaatag taagcactgt ttggattagc ctgccactgc cattgaagtc ccagatgttt
     1021 ttcctcaaaa tcatccgact gggcaatggc atatgacggc atatcctctt ctggcatata
     1081 ccagctttcc actggttccc caattccatc cccattttga tcctgaccaa tgaacggcca
     1141 tccatctgca aaacacatag gctgaagatg aacgatgcgt cccagttcga tcacatcctg
     1201 aaaatgaata aaccagtctc gtccatctga ggaagtaacc catccgccct gatgagggcc
     1261 attaactgct gaatttccct gatgcattac aattttgtac tcatacggtc cataaacgga
     1321 acgagaacga aggcaagact gccatccatc ttccactccg ccggaaggca taagaatata
     1381 ataatatcca ttttttttat acatcttcgg cccctctgtg gtaggagcta tctgttctcc
     1441 gtcgaaaatc agttttggtt cagaaagcaa accacggcag ttgggatcta tttccaccat
     1501 agagaggcgg tgtttcattc cacaacggct tttagcatag gcaaaaacca tatatgcctt
     1561 cccatcttca tcccagaacg gacatggatc gatccatccc ttagacttac acagaagatt
     1621 cagttcaaat tccccatagg gatctctgga acgggctacc agaatgccct catctactaa
     1681 aggaatgaat acgaagaaag ttccctcatg gtaccggatc gaagatgccc atgtacctga
     1741 tccatgagaa ggaaaatcat atttttcaaa aggaagtgag gaaacacaat ggttaatcag
     1801 ctcccaatgt accagatcct ttgaatgcag caaaggcact cctggaaggt aggtaaatga
     1861 agatgacacc atataatagt cttctccgac ccgaattaca tccggatcag aataatcagc
     1921 atataaaatt ggattctgat attgctgcat attcaatttc ctcctttacc ctttcagacc
     1981 agaagtagca attccctcta caaaatactt ctgggctgca aaaaacagaa tgatcaacgg
     2041 aattacagat accagcgcca tggccataac ctggttccac tgaatattgg atgttacgtc
     2101 aatgctgact ctaagagcca gagacagtgg aaacttggat acagtattaa tataaatatt
     2161 ggtattgaag aaatcattcc atgtccaaag gaactggaac agtcctgctg aaaacagggc
     2221 cggctttaac aatggaagaa gaatcttcat aaaacactgg aaaggaccac agccgtcaat
     2281 ataagcagat tcatccagat ctctgggaag tcctcttaaa aactgcacca tcataaacac
     2341 aaaaaacgga tagcatgcta ctgctgcggg agcataaaag gtcatatagg aatccagcca
     2401 gcccagcttg ttaaacaagg catagcgcgg aatgataata acagcatttg gcagcatcag
     2461 agttgctatc agcaccataa acagcgcctt gtttcctgga aaatcgaaac gtgcaaaacc
     2521 ataagccaca atagaacagg ataccaaagt caaaaaggtt gtaggaacca ccaaagcaaa
     2581 cgtattgata aaatactgag cataggtata agttcccgtt gtcttccatc ctttcacaaa
     2641 tgcttcaatc acccagctct ctggcagaag gcgcagactt ccaaaaattt cattgtttgt
     2701 cttaaaacag gcaaaaaaca tccatataac cggaaagagc atagctacac cgccgatagc
     2761 cagaaaggtg taggtaaacc cattttttat tcttttctct ctcatggctt cctcctaatt
     2821 atcataataa acccacttct ttgaagtagc aaacagcact gccgtcatga tcaggatcac
     2881 aatgaacaga atccaggaag tggcacatgc atatcccatc ttgaaatatt taaaagcatc
     2941 tgtatacaac ttcataccca gaacgtaggt acctttgttt ggtcctccgt tcgtaactac
     3001 aaaagcagag gtgaaattct gcagggcagt aatcagctgc ataataatat tgaaaaatgc
     3061 gatggaggta atcataggga gggtgatctt gaaaaacatt ctcggtttgg atgccccatc
     3121 aattctggca gcctcgtaaa gtgactgcgg aacctgtttt aaggctgaaa gaaacaatac
     3181 catggaggaa ccgaactgcc agatctccag catacatatg gtaactaacg ccatatctgg
     3241 tgtccccagc catttcactg gttcaatatt aaaacagccc agaagactat ttacaaaacc
     3301 atcgttcata aacagcacct tccacaaaat tgccacagct acggaaccgc tgagcaggga
     3361 cggaatataa tatactgtac gaataaaatt aatccccttt aaatttttat ttaagattac
     3421 tgcaatgatc agagcaagta caacctttcc aggtactgta atgaacgtat atttcaaagt
     3481 aacaattagt gaattccaaa attccttatc tttcgtaaaa agctttatat aattagcaaa
     3541 tcccacaaat tccggcttgc tgaaaatatc ataatctgta aatgaataca ccagggagca
     3601 aataaatgga tacagctgga ataacagaaa tccgaatatc cagatactca catacatgaa
     3661 tccaatctta ttccgtttgg tccagcctcc gtgcatggat cctgactttg cgcctttttt
     3721 tgccatcttg tcctccttat ccctgaaacg gcccgggaat gatacccgag ccgcctgcag
     3781 atccttttta tctcatattt ccgttgctgc tttttactgt taatccaaag cactcagata
     3841 atcatttaag agttcaatgg cattatcagc tgcctcttca ggagtgctct gtccgtaagc
     3901 aatgctttct actatttcct cctgaatcgc gtaaacctcg gcacttgtgg tatacccctt
     3961 gtcactcttt ccatttaatc ctgcagacaa atcaacagcc tttaccacag tctcatctaa
     4021 cagcccctta cctgcacata agcttctgcc agttgtagtt ggcggaatac ttcttacatc
     4081 ctttaatgtc tcctgggcag tttcattatt atagaagtag tttaagaatt ccacggcctc
     4141 ttttgcatgg ggcgaattct tatttacggt catatactgt ggcgtatctg caaagaaacc
     4201 atcattaatg cggtcatctg ccattggcat ctgcacaacc gtacactccg cttcagaaat
     4261 agcatccatc agcaaattaa tgttagagga tgcaccataa ccgaatacaa actttccatt
     4321 catccatttt ggattcgtat gaatctgtcc tttaaatgta gcactgtcct cagcaggctc
     4381 aaatacaccc caatcatagc aatccttaat gtatgtaaga gcctctacga gttcatccct
     4441 tgtaaatcca agcttctttt cactgtcgat aataaaggaa tttccagttt tctgacgaag
     4501 ataaggccgc agtacatact cacataaaat atgggtatct acgttcatat agtagtactc
     4561 tggattttct gcatgaagct tcttgccgtc ctctaacata ctctcccatg tgatctgttt
     4621 tgacagatcc acaccacaga catccgcagc ctctgtgtta gccacagttg ccagacaaga
     4681 cataccggtt ggaagtgcta acagctttcc atcaaatgta ccaaactgat caataaagtt
     4741 tttatcaaaa ccggatgtat caaaaatatc cttgtactca taaaaatcca caaagaaatc
     4801 acctcttgac acaaaatccg gcaaagaatc cacattctgc tgcataatat ccggtgcagt
     4861 accgtttgcc agcatggtag aaaccttatc aatgtaaccc tgatctgagt tcatctcagc
     4921 ctcaattttg atctcgggat gaagcttctg gtactggtca attactgcca gcgttgcctc
     4981 atttctctca tcgccgcccc accacataaa acgcagtgta atctgctctt gagaagcagt
     5041 ctcactcttc gcactctgat cttccgtttt ggtatcagaa gcagtgtttg ccgacggttc
     5101 attggtacaa cctgtaagaa caccagccgt cattatcccc gccatcacaa gacctgtaag
     5161 cttttttcca tgtttcatgt tgccatacct cccatatttg ctttcacgct ttgtttacat
     5221 tttgtctctt cctcatttga ctgcctttat tgtacataaa aaaaacactt ctgttaagaa
     5281 actaagctta acaaaaggta tctctgttta accaaacatc ctaatcggta tcattttcta
     5341 tgatttttct tcctgaacca cgatctatgg ttcaaaaaat gttttacaga tgatttttct
     5401 gtgaagattt tatataatat aattacacaa tgcctcggct atacagctcc ttcaaaagct
     5461 tgttttcaaa acacacattt tgtttaagaa agggcacgat ctatgtacca agtaatcatt
     5521 gcagacgacg aagcgaaaat acgaagcgga ctggccaatc tcttcccatg gaatcagctg
     5581 ggatttgaaa tcgctgcagt ttgttctaat ggaaaagagg ccttggattt cgccatggaa
     5641 aatccagtag atctggttct ctccgatata cggatgccca ttatggatgg tctggagctg
     5701 tcccggttgc ttctgccaaa aaagcccgta aaaatggtat ttttcagcgg ttatcaggat
     5761 tttaactatg cccgtcaagc catgaaaaac ggggtttccg attatctttt aaagcctgtg
     5821 aaatatgagg atctctctga ctgcctcacc agaatccggg aaactctgga catggaaaaa
     5881 ggactggcac aacctgaaga ggaaaattta agttactacg aaaaaattat caacactgtt
     5941 accagctatc tggatcagga ataccaaact gccagcttag agcaggcggc ccgtctggta
     6001 tctctaagcc ccaattatct ttctcggctg atgaaagatc actcggctct tagcttctcc
     6061 gattatcttc tcaaaagccg tatggaacat gctgccaata tgttaaagga catccatttc
     6121 aagcagtatg aaattgctta cagagtagga tatgataatc caaagaattt ctccagagcc
     6181 ttccaccagt atttcaaaat gactcctaca caatatcgta actgcagcgg agttacagaa
     6241 aaacgcaacg aaaaataaag gacattgatt cccatgagaa aacgcaattt tattaactgt
     6301 tttttttact attttgccat tattacgctc cctcttttaa tggtttttct tttcttttta
     6361 ttttttgtca ttcaggaaaa gaaacaggag attcaatcca atgcaaagcg ctccgctcag
     6421 gcggccaagg acaattattc tctggttatg gatactgccg ctacccagta tgatattctg
     6481 gcgcgcaatc caaggctctc tatttccctg agaaatttcc tttctcatga gaaagcgaat
     6541 tatctggatg tagttcttac caacagcatc ctttccaatt ttacctcgct caccaacaat
     6601 gccccttata ttgcctccgt atattattat ctggacgatt atgattctat tcttacctca
     6661 gacacaggag gaattgtaaa attatcccgg ttttccgact taagctggaa ggattcctat
     6721 gactcttcgg ataaagacca atggctggaa cgcagacaga tgcaccaata ctcctatgca
     6781 gaaccgattc ctgtacttac ctacttcaag aaattaaata tgtttcgcgg cgtagtaatg
     6841 gtcaatattt atgaggaaca gctcaaacaa ctgataacca ccagttccca gttagattct
     6901 ttctttatcc tggatcagaa tggaaatttt ctcctagaag cagggaaaac aaaacagttt
     6961 acccatgaag atcgcggcct tcttgcaaat aagctctcct ctgtttctct gtcccccttt
     7021 gaaggctggt ttcctttcag caatggttcc tgctatactc gaatgatacc agcagactgt
     7081 gatacctata ttgccgcctg tatcagctac tccaattatt atcgcgattt ttatccgctc
     7141 ctgctgcagt tgctcatggt gctggctgca atggtttttg tatccctatg gattgccttt
     7201 accatcacac gaaaaaattt tcagcaaatc gactactttg taaatctatt ctctgatgca
     7261 gagcgcggtg tattccgcca ggaaaaacct gactttctca gagatgagta tgatctggta
     7321 ctcaacaaca ttgtccagct gttcttaaac cagaccttcc taaaaaatca gctggcccta
     7381 aaggatcaga aacagaaatt ggcggagatg aaggcacttc agctgcagat caatccccat
     7441 tttctcttta acaccctgca aactctggat ttcaaggcgt tagaatatac cgggcaaccc
     7501 acagccatga atcagatgat ccaagccctg tcagatattc tcaaatattc cctccagaat
     7561 ccacattcta tggtgacttt aaaagatgag attgactatc tgaaaaaata tgacagtatt
     7621 caacgctgcc gctatgaaga caaatatatt ttgtactatg aatacgaaga aacccttgaa
     7681 aatcttccca taatgcggct gattttacag cccctgattg aaaacagcct gtaccacggc
     7741 atcaagccct tagatgattc aggttttatc aagcttcgta ttgtaagacg aaaaaatcat
     7801 ctgatcgttt ccgtcattga cacaggtgtg ggaatggacc gccaggcaat cacagcactc
     7861 tataaaagaa tagacaatcc cgacagcgac aacattggtc ttactaatgt aaacagccgt
     7921 ctggttatga aatacggccc ggaagcacat ttaaaaatca acagtaaaaa aggaatgggg
     7981 acttgcatat ctttccgaat accaatttaa aactgcagtt tttctaacct gaattcatat
     8041 agcttcttag tataaaaagg agtgagcatc cccccttcct atgctcactc cctgatattc
     8101 cacgccctct ttacggaacg cgggcctttt tacatcccca actgaaacaa tcccttcatt
     8161 gcaggataaa tctgcttaaa tacctgatac cgcttttcgt atttttccac caacccagtc
     8221 tccggctcaa cggtatcaac aatcttcacc agcttctctg ccgcttcttc caccgtttca
     8281 aattccccgc agccgactgc tgccagcata gcgcccccat atccggggcc ttcttcgctt
     8341 tcaatcacat cgaccttcag attcattaca ttcgcgataa ttttcttcca cagcagactc
     8401 tttgccccgc cgccgcagat tttggtacgc tcaatctgaa tccccagact tctggccact
     8461 tccagagaat ctctgagtcc aaatgccaca ccttccaaaa cagcctgcgt catgtcctct
     8521 cttgctgtat ccatggtcat tccgataaaa gcggctcttg ctttgggatc attgtgaggc
     8581 gaacgttctc ccatcaaata gggcagatag taaacatgat tttcacccag ttttgtaata
     8641 ctgtcctgct ctctttcata ctctgttgtc tttaaaattt catccatcca ccacttattg
     8701 caggaagcag cgctgagcat acatcccatc agatggaagc ttccatctgc atgagcaaag
     8761 gaatgcagtg cattgtgttc atccactccg aattttttac tggaaatgaa aatagttccg
     8821 gaagttccaa gtgaaatatt gcattttccc tctccgacgg ttccagtccc cacagcagcc
     8881 gctgcattgt ctccggctcc tgctacgacc ctcaccgctt ccgtaagccc cagcctgtct
     8941 gcaatttctt tcttcaataa accaaccact tcatagcttt caaacaattg cggaagctgt
     9001 tcttctttca ctccgcaaat atccatcatt tctctggacc agcatttatg ctccacatcc
     9061 aaaagcagca tccctgaagc gtcagaataa tccgtacaat ggattcccgt aagcatatat
     9121 gccagataat ccttgggcag cataattttg ctgatacggg caaaattttc cggctcattt
     9181 gctttcatcc agagaagctt cggtgcggta aagcctgcaa atgcgatatt tgctgtatat
     9241 ttagataatg cttcttttcc gattacttca ttgagatact ctgtttcctt cgctgttctt
     9301 ccgtcattcc agagaatcgc agggcggatc acctgatcca tctcgtccag aaccaccagt
     9361 ccgtgcatct ggccgccaaa tccgattcct gcaatctgtg ttctgtcaca ctctgctgtc
     9421 agctctttca ttccctccat agttttttca aaccagtcct gaggattctg ctcggaccag
     9481 ccgggatgag gaaaatacag gggatattcc tttgatacaa ttttatgaat ggttccgccc
     9541 tgatccatca gcagcagttt cactgctgat gtaccaaggt caatgcctat atataacatg
     9601 tttctctccc tttatttcca cggattctct gtaggatatc ttactcccgc aggctggtta
     9661 taaccgattt cctcaacagg aaccccaata tacttaaata cctcaaacaa agcctttcca
     9721 tggtttccaa atgcaactgc accatgatgt gggaaattct tttcaatcag cacatggcgg
     9781 taaaatcttc ccatttcagg aattgcaaat acaccaatgg aaccgaagga acgggttgca
     9841 acaggaagaa cctctccctg tgcaatatag gctctgagct tacagtcagc cgtgctctgc
     9901 agacggaaga atgtaatatt tcccggtgcg atatctccct ctaaggttcc ctgtgtcacc
     9961 tcttccggaa gagaacgtgc catgatcagc tgatatttca tttcacagaa ggacagtttt
    10021 tcagacggcg tatttccaca gtggaatccc ataaaggtgt cttgatgagt atatgggaat
    10081 ttgccttcta tttcttcttt atacatatct gccggcacat tattattgat atcaaggagg
    10141 gttaccgcat cttcgctgac aacggttccg atcaattcac tcaaagcacc gtagatatct
    10201 acctcacatg ataccgggat tccctgagcg gtcagacggc tgtttacata gcagggtaca
    10261 aatccaaact gtgtctggaa cgccggccag cactttcctg caattgccac atatttgcgg
    10321 taccctctgt gctcctcaat ccagtccttt aaggtaagct catactgtgc cagcttttca
    10381 aggatctccg gcttcttatt tccggcgcca agctcctctt ccatttcctt cacaaccgcc
    10441 gggatccgct catctccagc atgcttgtta aatgcctcaa acagatccag ctctgaattt
    10501 tcttcaattt caactcctaa attgtaaagc tgctgaatgg gcgcattgca tgcaaggaaa
    10561 tttaacgggc gcggcccgaa actgataatt ttcaaatcat tcaatgcgat cactgctctt
    10621 gcaaccggac ggaactctgc gatcatatcc gcacattctt cggcggttcc aatgggatac
    10681 tccggaatat aggctttgat atttctcagc ttcaggttat agcttgcatt gagcattccg
    10741 cagtaagcat cgccgcggcc ctgaatcaag ttgtccccac gctcctctgc cgcagccaca
    10801 aacatcttcg gtccgtcgaa atgctttgca agcagtgttt cagaaatttc aggtccaaaa
    10861 tttccaagat atacaaccag cgcattgcag ccggcagcct tgatatcttc cagcgcctgc
    10921 accatatgaa tctcactttc cacaatacaa atgggacatt cataaatatc cctatcccca
    10981 tatttttccc tgtatgcttt tatcaaagcc tctcttctgc tgacagacag actctccgga
    11041 aaacaatctc tgcttactgc tactactccg attttcaatt ctggcatatt tttcattgta
    11101 tgtacccacc ttcttattat tttaaactgt tatattttca ccctttaagc cgcagaatgc
    11161 acctacaatc ccagcttcct catgtgagat cagccatttg tttttggcag caagcagtct
    11221 gtcctctccc tctgcccctg ctgcaaaatc cagctccgta ttcgttcctt tcggcagcac
    11281 aaccacacat cggaaaccat tttcaccctc tgccgtacat ggcgcatagt gaagtgttgt
    11341 tgcatagacc tcaatcaccg tacctgcagg tacaaaaaaa gcctccacgc gagaagtgtc
    11401 atatgtaccg tctgcttcaa tatcctgctc ccgtccaagg agcagaacaa ggttgtttac
    11461 tgcaatatta atttcagaag aacgatgata ttccagcccg ttcagctttc tgttatcacc
    11521 attgcaatat ccgatttgaa ttggaaggcc gccaaatccg cggtttttca tctcctctgc
    11581 atcaggcaga gcctccattt cttccacaga cggaacatat acgacttctc ttgttaaagg
    11641 cgtatgctcc atttctgcca ggattccgtc tacatgatat cccgtcagaa ttttaccgta
    11701 ttttttaaat gattcctcat ttatattctg aatctgcatc attgtctcct ttcttattag
    11761 aatgttttct gttaagacca tgataacagc ttccttttat cttatcgacc ttttatttat
    11821 ccatacaact gcactttttt gtctttttta tatctgttcc gatgaatggg acgattctgt
    11881 ttttctccgc agatactcgc ttggaagcat cccgtatttc ttttgaaatg cctttgcaaa
    11941 agccctgctg cttggaaatc catggtgaaa ggccacttca ctgaccgtat gctccgtccg
    12001 gctcagttcc gtaaacgcat attctacacg tactccctgc agataatcct tataattgat
    12061 gcctgcatat ttttgaaaca ttcttgaaat atatgcagga gtatatccaa acacctcagc
    12121 cagcgcttcc aatgacagct cctcctgata atgttctttc atataattcg taatgggagt
    12181 aagtttattt aattttctgt tttgtttcag caattcatct tttacgtcca attcacggta
    12241 aacagaaaca agcatatata acagcatata atatccgctt tttacccgca tatcatatcc
    12301 cgcctctttt ttcccgtatg cagcaaacat ttcccggatc agatccacga cctggacatc
    12361 ctgctttttt gcttcatgtg taaatcgaat gtactgctcc ccggtaaagt aatcagaaaa
    12421 tgtttttaat ggaatctgta tcactactgt tttgtttggc tccggtgaat caatggcatg
    12481 tatctcattt gaattcacca gaacaaactc acctggattc agctcctgtt ccaaatcatt
    12541 caaatagaat tttaaatgac cctcaaacac cataaaaatt tcaatggagc gatgccagtg
    12601 tttatcccgg aaataatttc ccttctcccc ttcaaataaa aacatcttaa agggtaaatc
    12661 ttcatttggc actacaattt cgtggtttga ctgcatcttt caaaactcct gatcgtttca
    12721 ttctttttac tgtctttgtc ttaaatgtat tgtatcagta cactgatgtg acggctagtc
    12781 ccaaatgtat gaaaaatcat aacaaaaagg agtgagcata tccatttata tactcactcc
    12841 taaacctttc aaaatagatc tattgaatct gatccagcat tgattctcca atggttccat
    12901 ccggcatcct tacattgaaa ttttctgcaa tcgtagcccc tattgccgca aaagaatctg
    12961 cctcatttaa tgttcctgca cccttcagat cttttgcata aagaagcagc ggtacctgtt
    13021 ctctggtatg atcagttccc tgataagtcg ggtcatttcc atgatctgcc gtaatcatca
    13081 gcagatcctc atctcccatc agttccagca attctcccag cttttcatca aactgctcca
    13141 cttcttttgc atatccttcc gggtttctgc gatgccccca tactgcatca aaatctacca
    13201 gattcacaaa acataatcct tcaaattcac tatgttccat aatatccgct gtctgctgca
    13261 ttccatgaac agagctgtct gaatgatacg tttgtgtgat tccttctcca tcaaaaatat
    13321 catggatctt tcccacagca atcactgaca gaccattctc cttcaatgcg tccaaagctg
    13381 tttttccgga aggctttaaa gcataatcct ttcgattact ggtacgctta aattctccgc
    13441 gtttctttcc cacataaggc ctggctatca cacggcccac cttccactca tctttaagtg
    13501 taatttctct ggcaatttca cagcagcggt aaagttcttc cagatcaaat gtttcttcat
    13561 ttccgcaaat ctgaagcaca gaatcagctg aagtatatac gatcatatga ccggtagcaa
    13621 tctcttcttc tgccagttca tccagaatct ctgttccact ggaattctta tttccaatca
    13681 ctttatgacc ggtacgctgt tctaattctt taaccagttc ctctggaaac cctgtctctg
    13741 aaaatgtgac aaacggtttt gcagtctgaa ttcccatcat ctcccaatgt ccggtcatgg
    13801 tgtcctttcc acggcttttt tcattcagca aggcgtagta gcctaacggt tttacagccg
    13861 gctctactcc tttcagcgga tggagattag caagtcccag cttctgaagg tttggaatgt
    13921 tccactggcc cacatattct gcaatatgtc ccatcgtatc ggcgccttca tctccatatg
    13981 cttttgcgtc atgagcattt cccgcaccaa aagaatccat aacgatcaag aatattcgct
    14041 tgaatttcct cattccattt ttctcctttt tccttcactt atcgctctgt gggtaccatc
    14101 tgaatcgcac ctgtaggaca cacgatttgg caaagtccgc atcccgtaca tttctccgtg
    14161 cagaccaaag cttttcttgg actggtctcc agctgaattg cctgctttcc gccgtcactg
    14221 caggagacag cacatttccc gcacccaata catttatctg gatccactaa agctgccagc
    14281 tgctgcttta aagacagatt tcttgccgca gaagccatgt gattcaaaga atatccacgg
    14341 aactcatcca gagactgata tcctttttcc atcataaatt tttccagata agaaatcatc
    14401 tgcctgatta tgccatagcc tttatacata actgctgttg cgctctgcag tgtgtcagcg
    14461 cccagacaca gatactctaa tgcatccttt ccatctgtga ttcctccaac tgctgaaata
    14521 ccaaacggct tttcttttgc agcaagagcc attttataaa tacaatgttg agccataggt
    14581 ttaatgaacg ctccagacaa tcccatgggc gcactttgtc ccattatatc tggaatatgt
    14641 accatacgtt ccggattcag aggaccgatt ccatgaatag aattgattcc ggtcactgca
    14701 tctgctccgg cttcagccgc atacagagcc at
//
LOCUS       DBMDUH010000054        14618 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_2250_length_14618_cov_5.512434, whole genome shotgun sequence.
ACCESSION   DBMDUH010000054 DBMDUH010000000
VERSION     DBMDUH010000054.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 14618)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14618)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14618
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2250_length_14618_cov_5.512434"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="ACZPCG_06270"
     CDS             complement(<1..371)
                     /locus_tag="ACZPCG_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003523467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HIR1284708.1"
                     /translation="MKRLLSYMKAYRTESILGPLFKMLEASFELFVPLVVASMIDVGI
                     GGRDTGYVSLMGGLLVLLGMIGLACSLTAQYFAAKAATGAATALRNDLFTHISGLSYK
                     EIDQVGASALITRMTSDINQV"
     gene            complement(368..1234)
                     /locus_tag="ACZPCG_06275"
     CDS             complement(368..1234)
                     /locus_tag="ACZPCG_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002603784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HIR1284709.1"
                     /translation="MVKWIAAGREIYRKYKEDEMTVYAAQVSFFVLLSFVPFIMLLLT
                     AVQMIPAISQAEFMELVVAFTPEDYKSLAFRMVNDLSLKSPATMVSLTAVTALWSAGR
                     GMFSMARGLDRVWGKEEKYWYIISRLICCGYTIVFVAVCILSLILLVFGQLLRGFLNT
                     HFPFVGAVIGRLIDFRSLWFFLVLFFFFWGIYTFVPEKRLKAAKQIPGALFSALAWMV
                     FSLGFSIYFNRIGGRGYSYMYGSLAAIVILLLWLYFCMCILFMGAELNWFFVRLCSGE
                     WKKFDRNKEKRQ"
     gene            complement(1265..1543)
                     /locus_tag="ACZPCG_06280"
     CDS             complement(1265..1543)
                     /locus_tag="ACZPCG_06280"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HIR1284710.1"
                     /translation="MRKQIRRHIYFSGRVQEVGFRFTACLLARRLGLTGWVKNLWDGR
                     VEMEVQGTEDEIRRLIQGLQEGPYIRIEGMEAENLPLKEKEREFRETW"
     gene            complement(1558..2499)
                     /locus_tag="ACZPCG_06285"
     CDS             complement(1558..2499)
                     /locus_tag="ACZPCG_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015573460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HIR1284711.1"
                     /translation="MKLVIIEPLGVEKERLLSMAEEALKGRAEIIYYDTRATDTQELI
                     RRGQDADVIVVSNLPLNGEVIRGCRNLKLLSVAFTGVDHIDMEACKKQGVTVCNCAGY
                     STCAVADLVFGLLIGLYRNLISCDSVCRREGTKDGLVGFELEGKIFGVIGTGAIGLRV
                     AEIAKAFGCRVLAYSRTVKELPGITYVDMDRLLEESDIISLHVPLNDKTRGMIGAKEL
                     GKMKKTAVLINTARGPIVDSKALAEALKEGRLAGAGVDVFEQEPPVPSDHPLFSAPNT
                     IVTPHAAFATKEAMVKRAVTVFENVDFYLKGTPRNVM"
     gene            complement(2524..2892)
                     /locus_tag="ACZPCG_06290"
     CDS             complement(2524..2892)
                     /locus_tag="ACZPCG_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005347131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_function: GO:0043424 - protein histidine kinase binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HIR1284712.1"
                     /translation="MTIKECYEQIGADYEDVRSRLRSDLLIRKFFLKFPDDKSFGQLK
                     EALKSGDREGAFRGVHTLKGVAQNLGFVPLYEAADRLTEDLRPEGKGAALEEVNDAFA
                     VVNEAYEKIIKTAEQFIKEE"
     gene            complement(2917..4434)
                     /locus_tag="ACZPCG_06295"
     CDS             complement(2917..4434)
                     /locus_tag="ACZPCG_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004070144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HIR1284713.1"
                     /translation="MKSTEIRTKQRILIVDDSEMNRAILADMLGEGYEILEAENGLEA
                     VAYLKKLTTNIDLLLLDIMMPLMDGFEVLAVMNKYHMISDIPVIMISAESASSYVERA
                     YEMGVTDYISRPFNALVVQKRVENTLMLYAKQKRLVHMVADQIYEKEKNNDMMINILS
                     HMVEFRNGESGLHILHIHTMTEILLRQLCSKTDLYGLTREDIDRISTASALHDVGKIS
                     VPDSILNKPGRLTDEEFQIMKSHSVIGAQMLSQLPVYKDEPFIQTAYEICRWHHERYD
                     GKGYPDGLKGDEIPISAQIVSLADVYDALTSERCYKKAFSHQKAMEMILNGECGAINP
                     LLLECLEEVQELIREELQVYSPGTRHNRQIYETVERKLYNKELRMVGYNVGQIEIQEE
                     KKQFFLSKIPIVSFEYSAVTGITEMSGYAAEVFGMEPNVLDIRDESWINSAGTEAGKI
                     VKMVRETTPEDSEREDEIELLFNNVLHKCRVTVRTLWSKDQKPQYLGAVGVAVKI"
     gene            complement(4385..7246)
                     /locus_tag="ACZPCG_06300"
     CDS             complement(4385..7246)
                     /locus_tag="ACZPCG_06300"
                     /inference="COORDINATES: protein motif:HMM:NF012707.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1284714.1"
                     /translation="METEKKQTVHTNVKWKILAATAVFLTLILAFPGYCQEKKRIIRV
                     GVFSMEGYHMTDADGVRSGYGYDFLQKLSRYGNVVYEYKTANAENGGVLKMLKNGEVD
                     LVTSAVKRGQWEEDFVFSNQPVGTCGTMLTIKAGNEQIIPQDYSTYDGMRVGMIRQNI
                     RNENFKKFAEMKGFSYESVYYPDAAALYDGLQSGEVDAAVTTSLRAVKNEWMLDIFSR
                     EPFYVMVRKEDTKLLQWVDQAIAAMDQDEPGWRTLLSSQYYEDQLHGSLHLSQNEKKY
                     LDQLKKNGSVFTVAVNPDRFPYSYYEDGQMKGILPELFADIAERVGIRYQILISRTRD
                     EYKTLLDEKRPDLCIDCHDSFSRAEDLGYKLTDSYVSAGMSWLEFKDSKGEKEKIAVI
                     GNSVFPESLPETLGKDCLVYCDSFAECLKAMETGRADASYAYTYQVEKTVFDDIKDKY
                     KTSFTSHYQYFNIGISEDLDSILVRILNKGIKSMDSDFVDQVISRNTDYGTPPFSIAR
                     VAYQYPGLMILLCVMIAAIIAGLVWVYVQYKYHSRLMTALEEAKSASRAKSEFLSNMS
                     HDIRTPINGIMGMLEIARRSIGNPERMEDCLNKIQGAAGHLMALINDVLDMAKLESGK
                     AESSMESFELQEILDDCISITEGQLTGRRIEFIKETDYARGERAVGNPLHLRRILLNL
                     LSNAVKYTEDGGRIRFCVHKEAEDENSMLFVFTVEDTGIGMSPEYMDHLFEAFTQEHR
                     QARTTYRGTGLGMAITKKLVDQLGGVIEAESHQGEGSCFTVRLSFEKGEASDVHEERK
                     MTSCRAVLNGLKILVADDNELNREIAVCLLEEEGAHVTAAENGKQAAEVFENHPPGCF
                     DAILMDIMMPEMDGLEAAKRIRSCKGRPDGETVPIIAMTANVFAEDIAKTHGAGMDAH
                     LIKPIDLDQVCRALHKLVRKEKKDEEYGNKNKTENSDRR"
     gene            complement(7393..8916)
                     /locus_tag="ACZPCG_06305"
     CDS             complement(7393..8916)
                     /locus_tag="ACZPCG_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007490213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR1284715.1"
                     /translation="MRGVETRIQEIRHRIFREVARMAYHTEWPVDKRIEELPYKIIPG
                     EVGNFRNDVFLERAIVGERLRLAMGLPSRSAAEHAPLSDNIEAADKAETYYTPPLINV
                     IKFACNACKEKRVMVTDGCQGCLAHPCVEVCPKDAIHLDRTNGRSHIDEDKCIKCGKC
                     ATVCSYKAIIIQERPCAAACGMDAIRSDANGKADIDYEKCVSCGQCLVNCPFGAIADK
                     SQIFQVIRSIQSGEKVYAAVAPAFVGQFGPKVTPGKVRAAMKALGFTDVFEVAIGADL
                     CATQEAIDFLEEVPEKLPFMATSCCPAWSVMAKKLFPEYANCISMALTPMVLTARLIK
                     REHPDAKIVFIGPCAAKKLEAMRKSVRSEVDFVLTFEEMDGIFDAKHVDIENIEEDPE
                     GVNDASTDGRNFAVAGGVAKSVADVIHAKYPDKEVKIANAEGLKECRQLLKMATLGKY
                     NGYLLEGMACPGGCVAGAGTMQPVKKSQAAVNLYASKAKHKNSNETEHVKELDKLVY"
     gene            complement(9038..10996)
                     /locus_tag="ACZPCG_06310"
     CDS             complement(9038..10996)
                     /locus_tag="ACZPCG_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005330395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1284716.1"
                     /translation="MTQGEHILVCLSSSPANGTIIRAAARMAEAFKGEFTALYVETSQ
                     SQKMDEEDRSRLRGHIRLAKKLGAKIETTCGDDIAFQISEFARLSGVTKIVIGRSMAE
                     RRHLFGKPPLTEKLITNAPNMDIYIIPCQMAENRKFSRKNWQSALVLSVGDMIKSVLG
                     LAAASFIGILFEHLGFDEANIITVYVLAVLIISVVIRNQIYSLIASIVSVLVFNFLFT
                     DPKYSLMAYDRGYPVTFFVMFIAAFLTGTLASRMKNGSRQLAHVAFRTRVLFETNQLL
                     QQKKTRTEIVSATAHQLMKLLNRDIIFYLAKDGKLGEAQVFRIEGTDIPEDYVSEEEK
                     KAAQWAFDNNRHAGATTRTLSHASCLYLAVRINDTVYGVVGIVIKKDPLAMFENSILL
                     SILGECALALENEKNAWEKQEAAVLAKNEQLRSQLLRAISHDLRTPLTSISGNASNLL
                     SNGPSFDEATRQSLYQDIYDDAMWLINLVENLLSVTRLEEGRLNLNMSAELVDEVIKE
                     ALNHVDRKCTEHILTERCEDELLLAKMDAKLIVQVVINLVNNAIKYTEPGSHISVEAK
                     QEGKWAVISVADDGPGVPDENKEKLFEMFYSGANRIVDSRRSLGLGLSLCKSIVNAHG
                     GTISIRDNEPHGAVFTFTLPVEEVQLTE"
     gene            complement(10993..13053)
                     /locus_tag="ACZPCG_06315"
     CDS             complement(10993..13053)
                     /locus_tag="ACZPCG_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005952300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HIR1284717.1"
                     /translation="MEENRPNPELLLKKLRKEEEKREKEQRGKLKIFLGYAAGVGKTY
                     AMLEAAQTLRDRGEEVVAGYIEPHDRPATLAMAEGLETLPPFMVEYKGIRLREFDLDG
                     ALKRKPKLILVDELAHTNAPGLRHEKRYQDVKELLQAGISVYTTLNIQHLESLNDQIG
                     SITGIRVRERIPDGIFDQADQVELIDIEPSDLLQRLKEGKIYHAVQAERALDHFFAEE
                     KLIALREIALRRTADRVNRLAVMERENRGGADYYTGEHVLTCISPSPTCAKVIRTASR
                     LAYAFRGEFTALYVETPSLQHADPKVRQMVEENIHLAKALGARIATVFGEDIAGQIAE
                     YAKLSNVSKVVLGRTNHKILFGQTKGTLADHVAQYAPKLDIYIIPDIENGGTKKKRGF
                     YAGYGRGKRKKPETFMEGIGGDCVKCFLALAVSTGFGMMFQHFQMSESNIIMLYLLGV
                     LLAAMTVSRRGAVVVSSLLSVLLFNFFFVEPLYSFQMDGKEYPVTIAVMLAASLITSS
                     LMNRVRSHSREMAKKAYRTEILLDNSRRLRRIRDKKEAVKEVSGQIKKLLGLPVLFYL
                     YSGGGLEGPFFFPADGEGHMQEEEFLNQKERAVAMWSAARGKRAGACTHTLPDAKAIY
                     LPVKSGDRVYGVAGIALEERREIPPFEYSLLTAMLNEAGLVFERMEMMAASDEMKERK
                     GEII"
     gene            complement(13064..13702)
                     /gene="kdpC"
                     /locus_tag="ACZPCG_06320"
     CDS             complement(13064..13702)
                     /gene="kdpC"
                     /locus_tag="ACZPCG_06320"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HIR1284718.1"
                     /translation="MKQFLKTLKGAVVLTVLMAVLSSVIYPAVTTVLAQTVFKEKADG
                     SLIYADGKAVGSEVVGQQWSNPRFFKGRPSSVNYNVYTDEEKADGTYTGVASGTYNYG
                     PSNEALKERVETAVTEFKAEYKKATGEELTEEIPTDLLTASGSGLDPHISPASAKIQV
                     PIVAANTGFEEEELYQMVEAHTEHKALGIFGEERVNVLKLNLDLARKLGVTA"
     gene            complement(13746..>14618)
                     /locus_tag="ACZPCG_06325"
     CDS             complement(13746..>14618)
                     /locus_tag="ACZPCG_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016438368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HIR1284719.1"
                     /translation="GASEAIKARVRELNGTIPKDLEEIVTGISKLGGTPLVVCAGNVI
                     YGVIYLKDTVKPGLTERFERLRQIGIKTIMCTGDNPLTAATIAKEAGVDGFIAECKPE
                     DKIDAIKKEQAEGKIVAMTGDGTNDAPALAQADVGLAMNSGTAAAKEAANMVDLDSDP
                     TKILEVVEIGKQLLITRGSLTTFSIANDVAKYFAIIPAMFMMVIPQMGRLNIMGLSSP
                     YSAILSALIFNALIIPGLIPIAMRGVKYKPMSSERMLLKNMGIYGAGGIVVPFIGIKI
                     IDLIIAPLVGLMGL"
BASE COUNT         3580 a         4118 c         2942 g         3978 t
ORIGIN      
        1 tgtacctgat tgatatcgct ggtcatgcgg gtaatcaggg cagatgctcc cacctggtcg
       61 atttctttat aagacaggcc tgaaatatgc gtaaacagat cgttgcgcag agccgtagct
      121 gctccggtgg ctgccttggc tgcaaaatac tgcgcggtga gggagcaggc caggccgatc
      181 attccaagga gcaccagaag gcctcccata agagacacat agcccgtatc cctcccccct
      241 attccaacgt cgatcatact tgccaccacc agaggcacaa acagctcaaa gctggcctca
      301 agcatcttaa aaagaggccc caggatgctt tctgtcctgt acgccttcat ataactgagc
      361 aatcgtttca ttgccgtttc tccttgtttc tatcgaattt tttccattct ccactgcaca
      421 gccgcacaaa aaaccagttc agctctgccc ccataaaaag aatgcacata cagaaataca
      481 gccacaaaag gaggatcaca atggctgcaa ggctgccgta catatacgaa tatccccgtc
      541 ctccgatccg gttaaagtaa atggaaaagc ccagagaaaa aaccatccat gccaaagcgg
      601 aaaagagcgc tcccggaatc tgctttgccg cctttaagcg cttttccggc acaaaggtat
      661 aaatccccca gaaaaagaaa aacagaacca gaaaaaacca cagggatctg aaatcgatca
      721 gccggccgat cacggctccc acaaagggaa aatgtgtgtt caaaaacccc cgaagaagct
      781 ggccaaacac cagaaggatc agagaaagga tgcacacagc cacaaaaaca atggtgtagc
      841 cgcagcagat cagccggctg ataatgtacc agtatttctc ctcctttccc catacgcgat
      901 ccagccctct tgccatgctg aacatccccc ttccggccga ccataacgcc gtcaccgctg
      961 taagggaaac catcgtagcc ggagacttta aggaaagatc atttaccatg cgaaacgcca
     1021 gagatttata atcctccggc gtaaacgcta cgaccagttc cataaattcc gcctgcgaaa
     1081 tggccggtat catctggacc gcagtaagaa gcagcataat aaagggtacg aaggacagga
     1141 gcacaaaaaa agagacctga gccgcataga cggtcatctc atcctccttg tacttccggt
     1201 aaatttctct tccggcagca atccatttta ccatccttct ctcccttcat acaatatcca
     1261 ttttttacca ggtctcacga aattcccttt ctttctcctt taaaggaaga ttctctgctt
     1321 ccatcccctc aattcgtata taaggtcctt cctgcagccc ctggatcaga cggcggatct
     1381 catcctctgt tccctgaacc tccatttcaa cccggccgtc ccacaggttt ttcacccagc
     1441 cggtaaggcc caggcggcgg gccagaaggc aggcggtaaa gcggaatccc acctcctgaa
     1501 cccgtccgga aaaataaata tgtctgcgga tctgtttcct cattatatgg ccctttctca
     1561 catcacattc cgtggcgtac cctttaaata gaaatccaca ttttcaaata cagtcacagc
     1621 ccgctttacc atggcttcct tcgtagcaaa ggcagcatga ggcgtcacaa tcgtattggg
     1681 cgccgaaaac agcggatgat ccgacggcac cggaggttcc tgttcaaata catccacacc
     1741 ggctccggca agacgcccct cctttaatgc ctctgcgaga gctttcgaat ctacgatcgg
     1801 ccctctggcc gtattgatca gtacggctgt ttttttcatc tttccaagct ctttggcccc
     1861 gatcatgcct cgtgttttgt catttaatgg cacatggagg gaaatgatat cactttcctc
     1921 caggagtctg tccatatcca cataggttat acctggaagt tcctttacgg tacggctgta
     1981 agccagcacc cggcagccaa atgcctttgc gatctctgcc acccgcaggc cgatcgctcc
     2041 tgttccgata acaccaaaaa tctttccctc cagttcaaag cccaccaggc cgtccttcgt
     2101 cccttccctg cggcagacgc tgtcgcagga gatcagattg cggtacaggc cgataagcag
     2161 gccaaagacg agatccgcca ccgcacaggt agaatagccg gcgcaattgc acaccgttac
     2221 cccctgtttt ttacaggcct ccatgtcaat gtggtcgacc cctgtaaatg ccacggataa
     2281 aagcttcaga tttctgcagc cccggatcac ctctccattt aaaggcagat tcgacaccac
     2341 gatcacatcc gcatcctgcc cccttcggat cagctcctgc gtatccgttg ctcttgtatc
     2401 ataataaata atctctgccc ttccttttag ggcctcctct gccatggaaa gcagccgttc
     2461 cttttctaca cccaaaggtt ctatgatgac cagtttcatt tttccgtcct ccttttaatt
     2521 cttctattct tcttttataa actgctctgc cgtctttata attttctcat acgcctcatt
     2581 taccaccgca aaagcatcgt ttacttcttc caaagccgct ccttttccct caggacgcag
     2641 atcctctgta agacggtccg ccgcctcata aagcggaaca aatcccagat tctgcgccac
     2701 cccctttaag gtgtgaaccc ctcgaaaggc tccctcacga tccccggatt ttaacgcctc
     2761 ctttaactgt ccaaagctct tatcatcggg aaattttaaa aagaacttac ggatcagcag
     2821 atcgctgcgc agtctggaac gcacatcctc ataatcggct cctatttgtt catagcattc
     2881 ttttattgtc ataaatacca cctctattac gccttttcat atttttacag ccactcctac
     2941 cgcacccaga tactgaggtt tctgatcttt cgaccacaga gtgcgtaccg tcacacggca
     3001 cttatggagt acattattaa acaggagttc tatttcatcc tcccgctcag aatcctccgg
     3061 cgttgtttcg cgaaccattt ttacaatttt cccagcctct gtacctgccg aatttatcca
     3121 ggattcatct cttatatcca gcacattcgg ttccattcca aaaacctctg ccgcataacc
     3181 ggacatctct gtaatacctg tcactgcgct gtactcaaag ctgactatgg ggatttttga
     3241 aagaaaaaac tgttttttct cctcctggat ctctatctgg cctacattgt atcccaccat
     3301 ccgaagttct ttattataca gtttgcgctc cactgtctca tagatctgcc ggttatgcct
     3361 ggtgcctgga gaataaacct gcagttcttc gcggatcagc tcctgcactt cctcaagaca
     3421 ctccagaagc aagggattta tggcgccgca ctctccgttt aaaatcattt ccatggcttt
     3481 ttgatgggaa aatgcctttt tatagcagcg ctcgctggta agggcgtcat acacatccgc
     3541 caaagatacg atctgagctg aaatcgggat ctcatctccc tttaaaccgt ccggataccc
     3601 ttttccgtca tagcgctcat gatgccatcg gcagatttca taggcagtct ggataaaggg
     3661 ctcatcctta taaacaggaa gctgggaaag catctgggct cctatcacag aatggctttt
     3721 catgatctga aattcttcat ctgtaagacg gcccggctta tttaaaattg aatcaggcac
     3781 actgattttt cccacgtcat gcagcgctga ggctgtgctg atacggtcaa tatcttctct
     3841 tgtcaggccg tagaggtctg ttttggagca taactgacgc agcaggattt ctgtcattgt
     3901 atggatatga agaatatgaa gtccgctttc tccatttctg aattccacca tatggctcag
     3961 aatattgatc atcatatcgt tattcttttc cttctcataa atctggtctg ccaccatgtg
     4021 gaccagacgc ttctgtttag cataaagcat gagcgtattt tccaccctct tttgaaccac
     4081 aagcgcatta aaggggcggc tgatatagtc tgtaactccc atttcatacg cacgctccac
     4141 ataagaggaa gcgctttctg cagaaatcat aatcacagga atgtcggaaa tcatatgata
     4201 tttattcatc acagccagaa cctcaaatcc gtccatcaga ggcatcatga tatccaacag
     4261 gagcaggtca atattggtag taagcttttt taaatacgct accgcctcaa gaccattttc
     4321 cgcttctaaa atttcgtatc cctctcccag catatccgca aggatcgccc ggttcatttc
     4381 cgaatcatct acgatcagaa ttctctgttt tgttcttatt tccgtactct tcatccttct
     4441 tctcttttct aaccaacttg tgcagtgccc tgcatacctg atccaagtcg atgggtttta
     4501 taagatgggc atccattcct gcaccatgcg tttttgcgat atcctctgcg aacacattgg
     4561 ctgtcatggc gataatggga accgtctctc catccggccg tcctttacag gagcggatcc
     4621 tttttgcagc ctccagccca tccatttccg gcatcataat gtccataaga atggcgtcaa
     4681 agcagcctgg cggatgattt tcaaacacct ctgcagcctg ttttccattc tctgcagccg
     4741 tcacatgcgc accttcttct tccaaaaggc atacggcaat ttcccggttc aattcattat
     4801 cgtcagccac caggatctta agaccattca gaacagcccg gcaagatgtc attttccttt
     4861 cctcatgcac atctgaagct tctccctttt caaaggaaag cctgacggta aagcagcttc
     4921 cttctccctg atggctttct gcctcaatta ctcctcccag ctgatcgacc agttttttgg
     4981 taattgccat tcccaggccg gtgccccgat aggtagttct ggcctgtcga tgctcctggg
     5041 taaacgcctc aaataaatga tccatatatt ccggactcat gccgatcccg gtgtcttcca
     5101 ctgtaaatac aaaaagcatg ctgttttcat cttctgcctc tttatgaacg caaaagcgaa
     5161 tccttccgcc gtcttctgta tacttaactg cattgcttaa aagatttaaa aggatccggc
     5221 gcagatgaag aggatttccc actgcccgct cccctctggc gtaatccgtc tcttttataa
     5281 attctatcct cctgcctgta agctgtccct ctgtaatgga aatacagtca tccaggatct
     5341 cctgcagctc aaaagattcc atgctgcttt ctgcttttcc gctttccagc tttgccatat
     5401 caagcacatc gttgatcaga gccatcagat gacccgcagc tccctggatt ttattcagac
     5461 aatcctccat ccgctccgga ttccctatac tgcgccgggc gatttccagc attcccataa
     5521 tcccgttaat cggcgtacgg atatcgtggg acatattaga gaggaattcc gacttggccc
     5581 tactggcgct tttagcctcc tccagggctg tcatcaaacg gctgtgatac ttatactgta
     5641 catagaccca tacaagtccc gcaatgatcg ctgcgatcat aacgcataaa aggatcataa
     5701 gtccgggata ctggtaggcc actctggcaa tggaaaaagg cggggtaccg taatcggtgt
     5761 tcctgctgat aacctgatct acaaaatcag aatccatgct ctttataccc ttattcagga
     5821 tccggaccag aatactgtca agatcctcgg aaataccaat attaaaatac tgataatgcg
     5881 atgtaaacga tgtcttatat ttatccttta tatcgtcaaa cacagttttt tccacctgat
     5941 aggtatacgc ataggaagca tctgctcttc ccgtctccat cgcctttaaa cattctgcaa
     6001 aggaatcaca gtataccagg cagtcctttc ccagagtttc aggcaggctt tcaggaaaaa
     6061 cagaatttcc gatcacagcg atcttctctt tttctccctt ggaatcctta aattcaagcc
     6121 aggacatacc tgcacttaca taggaatccg tcagcttgta gcccagatcc tctgcccgtg
     6181 aaaagctatc atgacagtca atgcacaaat ccggcctttt ttcatctaaa agagttttat
     6241 attcatctct ggtcctggag atcaaaatct gataccgtat ccccacccgc tctgcaatat
     6301 ccgcaaaaag ttccggaagg attcccttca tctgtccgtc ttcatagtag gaatagggaa
     6361 agcggtccgg atttaccgcc accgtaaaaa cacttccatt ttttttcagc tgatccagat
     6421 atttcttttc attctggctc agatgaagcg atccatggag ctgatcttca taatattgac
     6481 tggaaagcag cgttctccat cccggttcgt cctgatccat agccgcgatg gcctgatcca
     6541 cccactgcaa aagctttgta tcttctttgc gtaccataac ataaaatggt tcccgggaaa
     6601 aaatatccag catccattca ttcttaactg cccgcaaact ggtagtaact gctgcatcta
     6661 cctcaccgct ttgaagtccg tcataaagag cggctgcatc tggataataa acactttcat
     6721 aggaaaagcc cttcatttcg gcaaattttt taaaattttc atttctgatg ttttgacgga
     6781 tcattcctac cctcatcccg tcataggtag aatagtcctg aggaatgatc tgttcatttc
     6841 ccgcctttat cgtcagcatg gtgccgcagg tacctacagg ctgatttgaa aacacaaaat
     6901 cctcttccca ttgcccgcgt tttaccgccg atgtcacaag gtcaacttct ccgtttttca
     6961 gcattttcag gacgccgcca ttctcagcgt ttgcagtttt atattcataa actacatttc
     7021 cataacggct cagcttctga agaaagtcat agccgtaacc gctgcgcact ccatctgcat
     7081 ccgtcatatg atagccttcc atggagaata ctcccacccg gatgatccgt tttttctcct
     7141 gacaatagcc aggaaacgcc aggatcaaag taaggaaaac agcggtggca gctaaaattt
     7201 tccatttaac gttcgtgtgc acggtctgtt tcttctccgt ttccaaaaca gcatcctctc
     7261 catttgcaga ttttttcttt tatctgtcca ttatatcgaa attttttctt ttaagcaacg
     7321 gcctgggggc aaaaacagaa aaatccccgg acaaaaggcc gtctccagcc gctgtccggg
     7381 gattttatct ttttaatata caagcttatc cagctctttt acatgctctg tttcatttga
     7441 gtttttatgc tttgccttgg atgcgtagag atttacggct gcctgggatt ttttcacagg
     7501 ctgcatggtt ccggctccgg ctacacagcc gccgggacag gccataccct ctaacagata
     7561 accgttgtac tttccaagag ttgccatctt aagaagctga cggcattcct ttaagccttc
     7621 tgcattggcg atctttactt ccttatccgg atattttgca tggatcacat cggctacgga
     7681 cttggccacg ccgcctgcca cagcaaagtt tcttccgtct gtggaagcgt catttacgcc
     7741 ctctggatcc tcttcgatat tctcaatatc cacatgcttt gcatcaaaga ttccatccat
     7801 ttcttcaaag gtcaggacaa aatccacctc gctgcggaca gattttctca tcgcttccag
     7861 cttctttgcc gcgcaggggc cgataaatac gatcttggca tccgggtgct ctctcttgat
     7921 cagtctggcg gtcagaacca taggggtcaa tgccatggaa atgcagttag cgtactccgg
     7981 gaacagcttc tttgccatca cagaccaggc cggacagcag gaagttgcca taaacggaag
     8041 cttttctggt acctcctcca aaaagtcaat ggcctcctgg gtcgcacaca gatccgctcc
     8101 gatcgctacc tcaaatacat ccgtaaagcc cagggccttc atcgccgcac gaacctttcc
     8161 cggtgttacc ttcggaccaa actggcctac aaaggccgga gcaacggcag catacacctt
     8221 ttctccggac tgaatggagc ggatgacctg aaagatctgg gacttatctg caatggctcc
     8281 aaatgggcag tttacaaggc actgtccgca tgacacgcac ttttcgtaat caatgtccgc
     8341 cttgccgttt gcgtcgctgc ggatggcgtc cattccgcag gcagcggcgc aggggcgctc
     8401 ctgaatgatg atcgccttat aggaacatac ggttgcacac ttgccgcact taatgcactt
     8461 gtcctcatca atatgggaac ggccgttggt acggtctaaa tgaatggcat ccttgggaca
     8521 gacctccacg cagggatggg ccagacagcc ctggcagccg tctgttacca taacacgttt
     8581 ttccttgcag gcattgcagg caaacttgat cacattgatc agcggcggcg tataataggt
     8641 ctccgcctta tcggctgctt ctatgttatc ggaaagaggc gcatgctctg ctgcactgcg
     8701 gctgggaaga cccatggcaa gacgaagacg ttctcctaca atggcgcgct ctaaaaatac
     8761 atcgttgcgg aaattcccaa cttcgccggg aatgatcttg tagggaagct cctcgatcct
     8821 cttgtccacc ggccattcgg tgtgataagc catgcgggca acctcacgga aaattctgtg
     8881 acggatctcc tggatcctgg tctctactcc tctcatattc tgtatcctca cttgtctatt
     8941 ttttatcaaa catattatag gcatggataa aggcgattgc aagccttttt aagcatacaa
     9001 tcgccttgta tcatcaaaaa tacacatttt ttccggttta ttctgtcagc tgtacctctt
     9061 ctacgggaag cgtaaaagta aacacggccc catgaggctc attatcgcgt atagaaatgg
     9121 tgcctccatg ggcatttaca atggatttgc acagggaaag ccccagcccc aggcttctgc
     9181 ggctgtccac gatccggttg gccccgctgt aaaacatttc aaaaagcttc tctttgtttt
     9241 catccggaac gccgggcccg tcatcggcca cggaaatcac cgcccatttt ccctcctgtt
     9301 ttgcttcaac tgaaatatgg gaaccgggct ctgtatactt gatggcgtta tttaccaggt
     9361 taatcaccac ctgaacgatc agcttggcat ccatttttgc caaaaggagc tcatcctcac
     9421 agcgttccgt aagaatatgc tccgtgcatt tccggtccac atgattaagc gcctctttta
     9481 tcacctcgtc caccagttcc gcagacatat ttaagttcag cctcccttcc tccagccggg
     9541 tgacggaaag aaggttttcc accagattga tcagccacat ggcgtcgtca taaatgtcct
     9601 gatacaggct ctgtctggtg gcctcgtcaa aggaggggcc gttggataaa agattgctcg
     9661 cattaccgga aatcgaggtc agcggggtac gcagatcatg ggaaatagcc cttaaaagct
     9721 gggagcgaag ctgctcgttt tttgccagga cggcagcctc ctgcttctcc catgcatttt
     9781 tttcattttc aagcgcaagg gcacattcac ctaaaattga aaggagaata ctgttttcaa
     9841 acatggcaag aggatccttc tttatgacaa ttcccaccac tccgtatacc gtgtcgttaa
     9901 tccgcacagc cagatacaga cagcttgcat gggacagggt tctggtggta gctcctgcat
     9961 ggcggttatt gtcaaacgcc cactgggcgg cctttttttc ctcttccgat acataatcct
    10021 ccggtatgtc tgttccttct atccggaata cctgagcttc tcccagcttc ccgtcctttg
    10081 ccagataaaa aatgatatcc cggtttaaaa gcttcatcag ctggtgggcc gtggcagata
    10141 caatctcagt ccgggtcttt ttctgctgta aaagctggtt cgtctcaaac agcacccggg
    10201 tccgaaaagc cacatgggcc agctgtctgg aaccgttttt catccgggaa gctaacgtcc
    10261 ccgtaaggaa agccgcaata aacatcacaa aaaaagtcac tggatagcct ctgtcataag
    10321 ccatcagcga atatttggga tctgtaaaca gaaaattaaa tactaaaaca ctgacaatcg
    10381 acgctatcag gctgtagatc tgattgcgga tcaccacaga tataatcaat actgcaagca
    10441 cataaacagt aataatatta gcctcatcaa agcccaaatg ttcaaacagg ataccgatga
    10501 aacttgccgc tgccagtccc aaaacactct ttatcatatc tccaacagat aatacaagag
    10561 cgctctgcca gtttttgcgg ctgaacttgc ggttttccgc catctgacag ggaataatat
    10621 agatatccat attgggagca ttggtaatga gcttttccgt cagaggcggc tttccaaaca
    10681 gatgcctgcg ctccgccatg cttcttccga tcacgatctt tgtcacgccg gaaagtctgg
    10741 caaactcaga tatctgaaac gcaatgtcgt ccccgcaggt ggtttcgatc ttggctccca
    10801 gcttctttgc cagacggata tgcccccgaa gccggctcct gtcctcctcg tccattttct
    10861 gggattggga agtttccaca tacagcgctg taaattcccc cttaaaggcc tccgccatcc
    10921 gggcagcagc ccggataatg gttccattgg ccggagaaga ggacagacaa actaaaatat
    10981 gctctccctg agtcatataa tttccccctt cctttctttc atttcatctg aagccgccat
    11041 catttccata cgttcaaata ccagtccggc ttcatttaac atggcggtta aaaggctgta
    11101 ttcaaacggg gggatctccc ttcgctcctc cagagcaatt cccgctacgc catatactct
    11161 atcaccgctc ttaacaggaa ggtaaatcgc tttggcatcc ggcagcgtat gggtacatgc
    11221 tccggccctc tttccccgtg cggcgctcca catcgccact gcccgttctt tctgatttaa
    11281 aaattcctcc tcctgcatat ggccctctcc gtcagccggg aagaaaaacg gcccttccag
    11341 tccgcctccg gaatacagat aaaaaagcac gggcagcccc agaagctttt tgatctgtcc
    11401 gctcacctcc tttaccgctt cctttttatc ccggattctc cgcagccttc tgctgttatc
    11461 taaaaggatc tccgtccggt atgccttttt tgccatttct ctggaatggc tgcggacacg
    11521 gttcataaga gaggaagtaa taagggaagc cgccagcatt acggcgatcg taaccggata
    11581 ttcttttcca tccatctgaa aactgtaaag aggctctaca aaaaagaaat taaacaaaag
    11641 cacgctgagc agagaagata cgactactgc cccccgtctg gaaacagtca tagccgctaa
    11701 aagaaccccc agaagataga gcatgataat attggattca gacatttgga aatgctgaaa
    11761 catcattcca aaccctgtag atactgccaa agccagaaaa catttcacgc agtctccccc
    11821 gatcccttcc ataaaagtct ccggcttctt tctttttcct ctgccgtatc cggcataaaa
    11881 acctcttttc tttttcgtcc ctccgttttc tatatccgga ataatgtaaa tatccagttt
    11941 gggcgcatac tgagccacat gatctgccag tgttcccttt gtctgtccaa ataaaatctt
    12001 gtgattcgtt cttccaagaa ctacttttga tacattgcta agcttggcat actctgcgat
    12061 ctgccctgca atatcctctc caaaaaccgt agcaattctg gctcccagag ctttcgccag
    12121 atggatattt tcctctacca tctgcctgac tttcggatct gcatgctgta acgaaggcgt
    12181 ttccacataa agggctgtaa attctccccg aaatgcatag gccaaacggg acgccgtacg
    12241 gattaccttt gcgcaggtcg gagacggcga aatacaggtc agcacatgct cacctgtata
    12301 gtaatcggcc ccgcctctgt tttcccgctc catcactgcc agacggttta ccctgtccgc
    12361 cgtacgtctt aatgcaatct cacgcagggc aatcagcttt tcctctgcaa agaaatgatc
    12421 cagcgcccgt tctgcctgaa ctgcatgata gatttttccc tccttcagtc tctgaagcag
    12481 atccgaaggc tcaatgtcga tcagttctac ctgatctgcc tgatcaaaaa tcccgtcggg
    12541 tatcctttcc ctgacccgga ttcccgtaat gcttccgatc tggtcattga ggctttccag
    12601 atgctggata tttaaggtgg tatatacgct gatcccggcc tgtaaaagct cctttacatc
    12661 ctgataccgc ttttcgtgcc gcagtccggg agcatttgta tgtgccagct catccactaa
    12721 gatcagcttt ggttttctct ttaaggctcc gtccagatca aattccctta aacggatgcc
    12781 tttatattca accataaagg gaggaagcgt ttccagcccc tctgccatag ccagagtagc
    12841 cgggcggtca tggggctcaa tatatccggc taccacctct tcccctctgt cgcgaagggt
    12901 ctgggctgct tccagcatgg cataggtctt tcccacgccg gccgcatatc ccagaaagat
    12961 cttaagcttt cccctctgct ccttctcccg cttttcttcc tctttacgga gcttttttaa
    13021 aagaagctcc gggttcggtc tgttctcttc catctaagcc tccttatgct gtcactccca
    13081 gcttccttgc cagatccaga ttcagtttca gcacattgac ccgttcctct ccaaaaatcc
    13141 caagcgcctt atgctctgtg tgggcttcta ccatctggta aagctcctcc tcctcaaagc
    13201 ctgtattggc tgccacaatg ggaacctgga tcttggctga tgccgggctg atatggggat
    13261 ccagtccgga accggaagca gttaaaagat ctgtcgggat ttcctctgtc agttcttctc
    13321 ctgtggcttt tttatattct gccttaaact ctgttacagc tgtttccacc ctttccttta
    13381 atgcctcatt ggatggacca tagttatagg ttcccgatgc cacgcctgta taggttccgt
    13441 ctgccttttc ctcatctgta tacacattat aattgaccga agaaggtctt cctttaaaaa
    13501 agcggggatt gctccactgc tgtcccacca cctcagagcc taccgccttt ccgtctgcgt
    13561 aaatcaagct tccatctgcc ttttccttaa acaccgtctg agccagtaca gtcgtcacag
    13621 ccggatatat aacagagctt aaaacagcca taagcacggt caggacaacc gctcccttta
    13681 atgtctttaa aaattgcttc atattcctct cctcctcata aaccgcccgg aaaccggggg
    13741 ctgtattaca gccccatcag tccaaccagg ggagcaatga tcaggtcaat gatcttgatt
    13801 ccgataaaag gtactacaat tccgcctgct ccgtagatcc ccatattttt aagcagcata
    13861 cgctctgagc tcataggctt atatttaacg ccgcgcattg caatggggat cagtccggga
    13921 ataatcagcg cattaaaaat cagtgcggat aaaatggcgc tgtaggggct tgaaagcccc
    13981 atgatattaa gcctgcccat ctggggaatc accatcataa acatggccgg gataatggcg
    14041 aaatactttg ccacgtcatt tgcaatacta aaagtagtca gagaacctct ggtaatcaaa
    14101 agctgctttc cgatctctac tacctcaagg atctttgtag ggtctgaatc cagatcaacc
    14161 atattagcag cctcttttgc cgcggccgta ccggaattca ttgccagccc cacatccgcc
    14221 tgagccagag ccggagcatc attcgttccg tcgccggtca ttgccacgat ctttccctct
    14281 gcctgctctt ttttaatggc gtcgatctta tcctccggct tacactctgc gataaagccg
    14341 tctacgcctg cctccttcgc aatggtagct gctgtcagag gattatcacc ggtacacatg
    14401 atggtcttga ttccgatctg gcgcaggcgc tcaaagcgtt ccgtcagtcc gggctttacg
    14461 gtatccttta agtaaatcac accataaatc acatttccgg cgcaaaccac taagggagta
    14521 ccgcccagct tggaaattcc tgttacaatt tcttccagat ctttagggat ggttccattt
    14581 aattccctta ctctggcctt gatggcctcg gaggcgcc
//
LOCUS       DBMDUH010000055        14520 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_2265_length_14520_cov_7.032943, whole genome shotgun sequence.
ACCESSION   DBMDUH010000055 DBMDUH010000000
VERSION     DBMDUH010000055.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 14520)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14520)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14520
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2265_length_14520_cov_7.032943"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            557..3127
                     /gene="secA"
                     /locus_tag="ACZPCG_06330"
     CDS             557..3127
                     /gene="secA"
                     /locus_tag="ACZPCG_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HIR1284720.1"
                     /translation="MNVIEKLFGTHSERELKMIRPIVAKIEGLRSEMMAKSDEELRDQ
                     TRIFKERLAKGETLDDLLPEAFATVREAARRTLNMEHFPVQLIGGIVLHQGRIAEMRT
                     GEGKTLVSTCPAYLNALAGKGVQIVTVNDYLAKRDAEWMGQVHRFLGLSVGVVLNDMN
                     SAQRKEAYACDITYVTNNELGFDYLRDNMAIYKEQLVLRDLDYCIIDEVDSVLIDEAR
                     TPLIISGQSGKSTKLYEVCDVLARQLERGTVSKEFSKIDAIMGEEIEETGDFVVDEKD
                     KVVNLTEQGVKKVEEYFHIDNLADPENLEIQHNIILALRANNLMFRDKDYVVKDDEVL
                     IVDEFTGRIMPGRRYSDGLHQAIEAKEHVNVRRESRTLATITFQNFFNKFNKKAGMTG
                     TALTEEKEFRNTYGMDAIAIPTNRPVQRKDLDDAVYKTKKEKYKAVVDEVVKAHEKGQ
                     PVLVGTITIETSELLSKMLTKRGIPHKVLNAKFHELEAEIVADAGIHGSVTIATNMAG
                     RGTDIKLDDESKALGGLKIIGTERHESRRIDNQLRGRSGRQGDPGESRFYLSLEDDLL
                     RLFGSDRLMHMFEAMGVPEGEQIEHKMLSNAIEKAQMKIEGNNYGIREQLLKFDEVNN
                     EQREVIYQERRKVLDGDNMRDLILKMITEIVENAVDMSIADDQTPDKWDLKELNSLLL
                     PVIPLKPVMLTDEQKKSMKKNELKHSIKEAAIKLYETKEAEYPDAEQIREIERVVLLK
                     VIDNKWMAHLDDMDALREGIGLQAYGQRDPVVEYKMQGYEMYEAMMASIQEETVRILF
                     HIRVEQKVEREPAAKVTGTNKDAVPVAPQKRAEQKIYPNDPCPCGSGKKYKQCCGRIK
                     "
     gene            3223..4360
                     /gene="prfB"
                     /locus_tag="ACZPCG_06335"
     CDS             join(3223..3297,3299..4360)
                     /gene="prfB"
                     /locus_tag="ACZPCG_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007156559.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HIR1284721.1"
                     /translation="MVELDNFKYTLSTYDKPLVEVRDSLDLDNKVRRINELDRSMEEP
                     GFWDDPDRSTRLVREAKNLKDEVGTFRELEQEYEDIQVMIQMGYEENDPSVIPDIQEM
                     LDHFTETLEKMRMKLLLSGEYDSYDAILRLNAGAGGTESCDWCSMLYRMYCRWAEKKG
                     FKLEVLDYLDGDEAGIKSVTIQISGDNAYGYLRSEHGVHRLVRISPFNAAGKRQTSFV
                     SCDVMPDIKEDIDIEINPDDIRIDTYRSSGAGGQHINKTSSAIRITHYPTGIVVTCQN
                     ERSQFQNKDKAMQMLKAKLYMLRQEEQAAKAAGIRGDVKDNGWGSQIRSYVLQPYTMV
                     KDLRTGEETGNVDAVLDGGLDPFISAYLRWMSLGCPDRNSRTED"
     gene            4531..4974
                     /locus_tag="ACZPCG_06340"
     CDS             4531..4974
                     /locus_tag="ACZPCG_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HIR1284722.1"
                     /translation="MEDKSKYFVVKQKAVPEVLLKVVEAKKLLESERAITVQEATDKV
                     GISRSSFYKYKDDIFPFYDNTKGKTITLVVQMDDEQGLLSDLLHVVAVYRANILTIHQ
                     SIPVNGVATLTLSVEIRENTGNVSGMIEELENLNGIHYVKILARE"
     gene            5022..6263
                     /locus_tag="ACZPCG_06345"
     CDS             5022..6263
                     /locus_tag="ACZPCG_06345"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HIR1284723.1"
                     /translation="MIKTAVMGYGTIGSGVTEILAENRDVIAAQAGQEIELKYVLDLR
                     EFPDSPIADKVIHEFKLIEEDPEIQIVVETMGDLNPAYPFVKACLMAGKHVVTSNKAL
                     VAAHGTELLAIARKKKVNFFFEASVGGGIPIIRPLYHCLMGERIEEITGILNGTTNYI
                     LTKMDKEGDTFENALREAQNLGYAERNPEADVEGYDTCRKIAILTAMAAGKEVDYQDI
                     YTEGITKITDVDFKYAEKMGVSIKLFGTGRIRDGKVSAYVAPVMINSSHPLYGVRDVF
                     NGIMVRGNMLGVSMFYGSGAGKLPTASAVIADIIEAAQNLKKNVPMGWGNEKQTIEPM
                     ENMRFRYFVRLAGSFRNKEQDIRRIFGHTEVIELYGMDEFAVLTPEMEEGAFRQAVAA
                     YDALAEAHSDFGIKQTIRAMM"
     gene            complement(6335..6754)
                     /locus_tag="ACZPCG_06350"
     CDS             complement(6335..6754)
                     /locus_tag="ACZPCG_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HIR1284724.1"
                     /translation="MNNIYKKQKNAPAPSEKFAVCSLICGIIGVVLSLFYLPVSLLIG
                     GNYPAGLICGAGGIVLALMARNADPSPRKTLPPKAIAGILLSGFAIMLTFFFFSQLIQ
                     FYDLLRDPVKGPQINQFLYQFQQRLMEQLQQAQPPVH"
     gene            6924..9080
                     /locus_tag="ACZPCG_06355"
     CDS             6924..9080
                     /locus_tag="ACZPCG_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HIR1284725.1"
                     /translation="MDIIKTLAEELQIGRHQAEAAVKLIDEGNTIPFIARYRKEATGS
                     LNDEILRALDERLRYLRGLEDRKNQVIATITDQGKMTPGLEQQIRSAATLVAVEDLYR
                     PYKPKRRTRAMIAREKGLEPLADLIFLQEAKEPLEETAASYVSEEKGVESPEAAIQGA
                     MDILAERISDHPGYRTWIRNITMNKGLLQSSAKDEKVKSVYETYYNYEEPVKKAASHR
                     ILALNRGESEKILTVKVIPPVDQIMGYLEKQVIVKNNPYTTPVLKAVVKDSYDRLTGP
                     SIEREIRSEITEKAEDGAIRVFGKNLEQLLMQPPITGRVVLGWDPAFRTGCKLAVVDS
                     TGKVLDTIVIYPTAPQNKVEESKKILKEMIHKYNISLISVGNGTASRESEQVIVELLK
                     EIKEPVKYVIVNEAGASVYSASKLATEEFPAFDVGQRSAVSIARRLQDPLAELVKIDP
                     KSIGVGQYQHDMNQKKLGAALEGVVEDCVNRVGVDINTASASLLEYVSGINKTLAKNI
                     VAYREENGAFKSRKQLLKVAKLGPKAFEQCAGFMRITGGAEPLDGTSVHPESYGAAKA
                     ILQSLGYREEELARGGIPGISRKIRDYKKMAEEVGVGEITLRDIAGELEKPARDPRDE
                     MPRPVLRSDILEMKDLKAGMVLKGTVRNVIDFGAFVDIGVHQDGLVHISQMTDRYIRH
                     PMDAVSVGDVVDVKILAVDLEKKRISLTMKGVKDGN"
     gene            9070..9498
                     /gene="tsaE"
                     /locus_tag="ACZPCG_06360"
     CDS             9070..9498
                     /gene="tsaE"
                     /locus_tag="ACZPCG_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006774415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HIR1284726.1"
                     /translation="MVIETFDPKETYDLGVKMGEQARAGQVICLNGDLGVGKTVFTQG
                     FAAGLGIAGPVNSPTFTIVQQYEDGRLPLYHFDVYRIGDISEMDEIGYEDCFYGQGVT
                     LIEWSELIREILPEHVTEVTIEKDMEKGFDYRRIRVEEKE"
     gene            9495..10256
                     /gene="tsaB"
                     /locus_tag="ACZPCG_06365"
     CDS             9495..10256
                     /gene="tsaB"
                     /locus_tag="ACZPCG_06365"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HIR1284727.1"
                     /translation="MRVLGIESSSLVASVALVTDDILTGEYTANFKKTHSQTLLPMLD
                     ELAKLLELDLNTVDAIAVAGGPGSFTGLRIGAATAKGLGLALKKPLIHVPTVDALAYN
                     MWGASGLICPIMDARRRQVYTGLYQMEQGIHVQMEQQPMDMGDLAEELNRRGERVIFL
                     GDGVPAYREMIQEMVKVPFEFAPAQMNRQRAASVAALGSLALKEEGDGCGYHSAFLVS
                     AGEFNPDYLRKSQAERQREAELSPEGAAGKLPGDY"
     gene            10288..10737
                     /gene="rimI"
                     /locus_tag="ACZPCG_06370"
     CDS             10288..10737
                     /gene="rimI"
                     /locus_tag="ACZPCG_06370"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007707489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 - protein-N-terminal-alanine
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HIR1284728.1"
                     /translation="MNQNLRVRRMEAGDLLRAAALEQSSFSQPWSEKLLAEGLNSPLD
                     TYFVCTCENEIAGYCVLRVLADEGELQRIAVFSEYRRQGVGRKLMEAMLAAAREEGAS
                     SVSLEVRESNVGAMRLYEEYGFCQEAVRKKYYQEPEEDAVIMWNRSL"
     gene            10900..11142
                     /locus_tag="ACZPCG_06375"
     CDS             10900..11142
                     /locus_tag="ACZPCG_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284729.1"
                     /translation="MKKYNKNGQLETLICNGCGKKLAVSQGIIREGAISIDHVWDYFS
                     EKDGQVHHFDLCEECYDQMINRFRIPVSIEEQTEYL"
     gene            11283..12194
                     /locus_tag="ACZPCG_06380"
     CDS             11283..12194
                     /locus_tag="ACZPCG_06380"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity
                     producing 5'-phosphomonoesters, hydrolytic mechanism
                     [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HIR1284730.1"
                     /translation="MLDICLLGTGGMMPLPYRWLTSMMARCGGSNLLIDCGEGTQVAL
                     KEKGWSPKPIDTICFTHYHADHISGLPGLLLTMGNAERTEPLTLIGPRGLERVVNALR
                     MIAPELPFELRMIELTENRQQLTAGPYIIDAYKVNHNVLCYGYTIRIPRKGRFDVERA
                     KEQQIPQKFWNRLQKGEEIECEGRLLTPDMVLGPDRRGLKVTYCTDTRPIPVIAEYAA
                     GSDLFICEGMYGEKEKASKAREYKHMTFYEAAALAKEAKPGQLWLTHYSPSLTRPDDY
                     MDEVRKIFPRAKAARDGWTMELEFDEE"
     gene            12198..13232
                     /gene="tsaD"
                     /locus_tag="ACZPCG_06385"
     CDS             12198..13232
                     /gene="tsaD"
                     /locus_tag="ACZPCG_06385"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HIR1284731.1"
                     /translation="MNVNHTEDVLILAIESSCDETAASVVKNGRTVLSNVISSQIATH
                     TVYGGVVPEIASREHIKAINYVIERALAEAETTLDDITAIGVTYGPGLVGALLVGVAE
                     AKAIAYAARKPLIGVHHIEGHVSANFIENKDLEPPFICLIVSGGHTHLVVVKDYGEFE
                     IIGHTRDDAAGEAFDKVARAVGLGYPGGPKIDKAAKEGNPHAIEFPRGKVADSPYDFS
                     FSGLKSAVLNHINHAKMTGEEICVPDLVASFQNSVVDSLVSRAIHAAKEYGYSKLAIA
                     GGVASNTALRAAMKKACEENGIAFYHPSPVFCTDNAAMIGAAAYYEYIRGNRSGWDLN
                     AVPNLKLGER"
     gene            13233..13970
                     /gene="ispD"
                     /locus_tag="ACZPCG_06390"
     CDS             13233..13970
                     /gene="ispD"
                     /locus_tag="ACZPCG_06390"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004840929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HIR1284732.1"
                     /translation="MKKFCTAVVLAAGRGSRMGTAVAKQYLTLGDKPVVVYSLEAFQA
                     SPVIDEIILITDKDHIEYCWEKLVHPYGITKVTTVAPGGKERYESVWKALCLLCGKNS
                     RNEAGASAGDGYVFIHDGARPFITEDIIERVYKDTEKWDACVVGMPVKDTIKQTDENG
                     VILDTPDRSRIWQAQTPQVFSVPLIVEAFQRQLSQDCRRVTDDSMIVENQMGVKVHMT
                     EGGYTNIKITTPEDLAVAEAILTQYKK"
     gene            14137..14222
                     /locus_tag="ACZPCG_06395"
     tRNA            14137..14222
                     /locus_tag="ACZPCG_06395"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14172..14174,aa:Ser,seq:tga)
     gene            14280..14368
                     /locus_tag="ACZPCG_06400"
     tRNA            14280..14368
                     /locus_tag="ACZPCG_06400"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14314..14316,aa:Ser,seq:gga)
BASE COUNT         4229 a         2804 c         4051 g         3436 t
ORIGIN      
        1 ggcttataaa tgtcaagagg aaaagcagaa aaaatgtaaa aaacatgtaa atttatcaga
       61 tgtgtacaaa aaaaacagga tctgaaatgg aaagaaaatt ggtaaaagcg gggacttata
      121 aacttgtgga taatgtggat aagtcggtgc ataagtaaaa aacaacgaaa ataaaaggaa
      181 aatgaggtgg ggataacttt gggggaaatg tttgtggata actgtggata atgtggatag
      241 aagaaggaac gaacgtgtgg tttgtgcata ttgttaaaag ggcaggaatg tacaggaacg
      301 gagggaaaaa caggatgctt cgcagtaccg gggaggctga ttgccgggaa atgtaaattt
      361 tttatgaatg agttgaataa atcagcgtct ggtgatacaa tatattggat tatatcatag
      421 catactgcgt gcatttatca gggaatagag agtattttca gacagattat aaacagacag
      481 cagctttccc ggcacgacag ccccagtttg ggagagtata gacttttata taaggaagaa
      541 gttaggagag tagaccatga acgtaattga gaaactgttc gggactcata gtgaaagaga
      601 acttaagatg atccggccga tcgttgccaa gattgaggga ctgcgttctg agatgatggc
      661 aaagtcagac gaagagctgc gcgaccagac ccggattttt aaagaaagac tggcaaaggg
      721 cgaaacactg gatgatcttc tgccggaggc atttgctact gtgcgggagg ctgcaagaag
      781 aacgctgaat atggagcatt tccctgtaca gctcatcggt ggtattgtac ttcatcaggg
      841 ccgtattgcc gagatgcgta caggagaggg aaagacgctg gtatccacct gtcctgctta
      901 tttaaatgcg ctggccggaa aaggtgtaca gatcgtaacc gttaatgact atctggcaaa
      961 gcgagatgca gagtggatgg gccaggtaca tcgtttcctg ggactgagtg taggcgtggt
     1021 tttaaatgac atgaacagcg cccagcgcaa ggaggcatac gcctgcgaca ttacctatgt
     1081 gacaaataac gagctgggat ttgactacct tcgtgacaat atggccattt acaaggagca
     1141 gctggtgctc cgcgatctgg actactgtat tatcgatgag gtggactctg tgctgatcga
     1201 cgaggcgaga acccctctga tcatttccgg acagagcgga aaatccacaa agctttatga
     1261 ggtctgcgat gtactggccc gtcagctgga gcgcggaacg gtttctaagg aattttccaa
     1321 gattgatgcc attatgggtg aggagattga ggagaccggc gatttcgtag ttgatgagaa
     1381 ggataaggta gtaaacctta cggagcaggg tgtgaaaaag gtagaggaat atttccacat
     1441 tgataacctt gcagatccgg aaaatctgga gatccagcac aatatcattc tggctctgcg
     1501 cgccaataac ctgatgttca gagataagga ttacgtggta aaggacgatg aggtactgat
     1561 cgtagatgag tttacaggac gtatcatgcc gggccgccgc tattccgacg gacttcatca
     1621 ggcaatcgag gcaaaggagc atgtaaatgt gcgccgtgaa agccgtacac tggctaccat
     1681 caccttccag aatttcttca acaagttcaa taagaaggca ggtatgaccg gtactgctct
     1741 tacggaggag aaagaattcc gcaataccta tggcatggat gccattgcta ttccaactaa
     1801 ccgtcccgtt cagcgtaagg atctggatga tgcggtatac aagacaaaga aggaaaaata
     1861 caaagcagtt gtagatgagg tggtaaaagc ccatgaaaag ggacagcctg tattggttgg
     1921 taccattacc attgaaactt ctgagctttt aagcaagatg ctcacaaaga gaggcattcc
     1981 tcataaagtc cttaacgcta aattccatga gcttgaggcc gagatcgtgg cagatgcagg
     2041 tatccacggc tccgttacca ttgctacgaa tatggctggc cgtggtacgg atattaagct
     2101 ggatgacgag tcaaaggctc tgggcggctt aaagatcatc ggtaccgagc gccatgagtc
     2161 ccgccgtatt gataatcagc tccgcggccg ttccggccgt cagggcgatc cgggtgagtc
     2221 cagattctat ctgtctctgg aggatgacct gctgcgtctc ttcggttcag accgtctgat
     2281 gcatatgttt gaggccatgg gcgtgccgga gggcgagcag attgagcaca agatgctgtc
     2341 aaatgccatt gagaaggcac agatgaagat cgagggcaat aactacggta tccgtgagca
     2401 gctcttaaag ttcgatgagg tgaacaatga gcagcgagag gttatttatc aggagcgccg
     2461 taaggtgctg gacggcgaca atatgcgcga tctgattctt aagatgatca cagagatcgt
     2521 agaaaatgcc gtggatatgt ccattgcaga tgatcagact ccggataagt gggatttaaa
     2581 ggaattaaac agccttcttc tccctgtgat tcccttaaag ccggtgatgc ttacggatga
     2641 gcagaagaag tccatgaaga aaaatgaatt aaagcacagc atcaaggaag ctgccattaa
     2701 gctgtatgaa acaaaggagg cagagtatcc ggatgccgag cagatccgtg agatcgagcg
     2761 cgtagtcctt ttaaaggtaa ttgacaataa gtggatggct catctggatg acatggacgc
     2821 tctccgcgag ggtatcggtc ttcaggcata cggtcagaga gatccggtgg ttgaatataa
     2881 gatgcagggc tatgaaatgt atgaagctat gatggcttcc attcaggagg agaccgttcg
     2941 catcctgttc catatccgcg tagagcagaa ggtagagcgc gagcctgctg ccaaggtgac
     3001 aggtaccaat aaggatgcgg ttcctgtagc gccccagaag agagctgagc agaagatcta
     3061 tccgaatgat ccctgtccct gcggctccgg caagaagtat aagcagtgct gcggacgcat
     3121 taaatagtgc gcagtcattt ataaatgatc aaaagtaact attcctccga tgggagaact
     3181 gaatagttgc cattaaaata taaaaagaaa gcgggtgaag ctgtggttga gttagataat
     3241 ttcaaatata cgttatcaac gtatgacaaa ccattagtgg aagtgaggga ttcactttga
     3301 cctggataac aaggtcagac gaatcaacga attagaccgg tcgatggagg agccggggtt
     3361 ctgggatgat cccgaccgtt ctaccagact ggtgagagaa gcaaagaatt taaaggatga
     3421 ggtgggaacc ttccgcgagc tggagcagga gtatgaggat attcaggtta tgattcagat
     3481 ggggtatgag gaaaatgacc cttctgtgat cccggatatc caggagatgc tggatcattt
     3541 tacagaaact ctggaaaaaa tgcggatgaa gctccttctt tctggcgagt atgacagcta
     3601 tgacgcgatc ctgcgtctga atgcgggagc cggaggcaca gagtcttgtg actggtgcag
     3661 tatgctgtac cgtatgtact gccgctgggc agagaaaaag ggatttaagt tagaagttct
     3721 ggattatctg gacggcgatg aggcgggcat taagtctgtt accatccaga tcagcggaga
     3781 caatgcctac ggttatctgc gttccgagca cggagtccac cgcctagtgc gcatttctcc
     3841 cttcaatgcg gcgggaaaac gccagacatc ctttgtatcc tgtgatgtga tgccggatat
     3901 caaagaagat attgatattg agatcaatcc cgatgatatc cgtatcgata cctaccgttc
     3961 cagcggtgcc ggcggacagc atatcaataa aacatcttca gctatccgta ttactcatta
     4021 tcccaccgga attgtggtaa cctgtcagaa tgagcgttcc cagttccaga ataaggataa
     4081 ggctatgcag atgttaaagg caaagctgta tatgctgcgc caggaggagc aggcagctaa
     4141 ggctgcaggt atccgcggag acgtaaagga caacggttgg ggaagccaga tccgttccta
     4201 tgtgctccag ccttatacca tggtaaagga tctgcgcacc ggagaggaaa cgggcaacgt
     4261 agacgccgtg ctggacggtg gactggatcc ctttatcagt gcttacctgc gctggatgag
     4321 tctgggatgt ccagatcgga attcacgcac agaagattaa aaacagagaa ttatactgga
     4381 aaagccgggc aatagtccgg cttttttctg acttcattta aattccggag aaaaacagtt
     4441 gcatttctat ggaaaatatg ataccatctt ccacgtgggt acatatgtac atctgatgcg
     4501 gacacaaaaa aggaaggaag acaagagtac atggaagaca aaagcaagta ttttgtggtg
     4561 aagcagaaag ctgttcctga ggtactttta aaggtggtgg aggcgaagaa gctccttgaa
     4621 agcgaacgcg cgattacagt tcaggaggcc acagacaagg tggggatcag ccgcagctcc
     4681 ttctacaagt acaaggatga tatttttcct ttttatgata atacgaaggg aaagaccatc
     4741 actctggtgg tacagatgga tgatgaacag gggcttttaa gcgaccttct tcatgtagtt
     4801 gctgtttacc gggccaatat tctgacaatc catcagagta ttcctgtaaa tggagtggct
     4861 acgctgacgc ttagtgtaga gatccgcgag aatacgggaa atgtttccgg tatgatcgag
     4921 gagcttgaaa atctaaatgg aatccattat gtaaaaatat tggcaagaga ataggcctgt
     4981 attttcaggg agaagtataa aaaggcaggg agcagaggat tatgattaag acagcagtaa
     5041 tgggatatgg aactattggc tccggtgtga cggagattct ggcagaaaat cgggatgtga
     5101 tcgctgcaca ggcagggcag gagatagagc tgaagtatgt gctggatctg cgggagtttc
     5161 ccgacagccc catcgcagat aaggtgatcc atgagtttaa gctcattgaa gaggatccgg
     5221 agattcagat cgtggtggaa accatgggag atctaaatcc ggcgtatccg tttgtaaagg
     5281 cctgcctgat ggcaggaaag catgttgtta cttccaataa ggctctggta gctgctcacg
     5341 gcacagaact tcttgccatt gcgcgaaaaa agaaggtaaa tttctttttt gaggccagcg
     5401 tgggaggcgg aatccctatt atccgtcctc tgtatcattg tctgatggga gaacgcattg
     5461 aggagattac gggcatttta aacggtacga ccaactatat tctcacaaag atggataaag
     5521 agggagatac ttttgaaaat gctttaagag aggctcaaaa tctgggatat gcggagcgca
     5581 atccggaggc ggacgtagaa ggctatgaca cctgccgcaa gattgccatt cttacagcca
     5641 tggctgcagg caaagaggtg gattatcagg atatctacac agaaggaatt acaaagatca
     5701 cagatgttga ttttaaatat gcggagaaaa tgggcgtttc cataaagctg ttcggcaccg
     5761 gccgcatcag agacggaaag gtaagtgcct atgtggctcc ggtaatgata aacagcagtc
     5821 atcctctata tggagtaaga gacgtattta acggtattat ggtccgcgga aatatgcttg
     5881 gcgtgtctat gttttacggc agcggagcgg gaaagctccc tacagccagc gcagtgattg
     5941 cagatatcat tgaggctgct cagaacctga agaaaaatgt tcctatggga tggggaaatg
     6001 aaaagcagac catagagcca atggagaata tgcgttttcg ttactttgtc cgtttggcag
     6061 gttccttccg caataaggaa caggatatca ggcgtatttt cggccatacg gaggtcatcg
     6121 agctttacgg aatggatgaa tttgccgttc tcaccccgga gatggaggag ggcgcgttcc
     6181 gacaggctgt agcggcctat gatgcgcttg cagaggcaca ctcggatttt ggaatcaaac
     6241 agaccatccg ggctatgatg tagagttgaa tacagataat aaaaacttct gccaatgtga
     6301 aaacggcaga agttttttta aacattttta aaaatcagtg taccggaggc tgtgcctgtt
     6361 ggagctgttc cataaggcgc tgctggaact ggtagaggaa ctgattgatc tgtggaccct
     6421 tcaccggatc gcgcagcaga tcataaaact ggatcagctg agagaaaaag aaaaaggtca
     6481 gcataatagc aaaaccgctt aaaaggattc cggcaatggc cttgggcgga agggttttgc
     6541 gggggctggg atctgcattc cgggccataa gggccaggac aatgccgcct gcgccgcaga
     6601 tcaggcctgc aggataatta ccgccgatta aaagagaaac aggcagataa aacaggctta
     6661 agacaacgcc gatgataccg cagatcagag agcatacggc aaatttctcc gagggagcag
     6721 gtgcattctt ttgtttttta tagatattgt tcatgatatt aaatgcaata gcctttcttt
     6781 gagatagtga tagtcaacat ggcttattct aacatgaaaa aatcatcctg tccagtgtca
     6841 catcttggtg agtcttgcat aagaaggcag gatgaattat aataaaacag ttactgagag
     6901 aataaagaca gcgaggaaaa ttaatggata ttataaaaac actggccgaa gaattacaga
     6961 tcggccgcca tcaggcagag gctgccgtaa agctgatcga tgaaggaaat accattccgt
     7021 ttattgccag ataccgcaag gaggccacag gttccttaaa tgatgagatc ctgcgtgctc
     7081 tggatgaacg tttaagatat ctgcggggac tggaagaccg caaaaaccag gtgatcgcca
     7141 cgattacaga tcagggaaag atgacgcctg gactggagca gcagatccgc agtgcagcta
     7201 ccctggtagc agtggaggat ttataccgcc cctataagcc aaagcgtcgc acaagagcga
     7261 tgattgcccg tgagaagggg ctggagccgt tggcagacct gattttttta caggaggcga
     7321 aggaacctct tgaagagaca gcggcttcct atgtttcaga ggaaaaggga gtggaatcgc
     7381 cggaagctgc cattcagggt gccatggata ttctggcgga gcggatttcc gatcatccgg
     7441 ggtaccgcac ctggatccgt aatataacga tgaataaggg acttcttcag tccagtgcaa
     7501 aggatgagaa ggtaaagtcc gtttatgaga cctattataa ttatgaggag cctgtaaaaa
     7561 aggctgcgag ccatagaatt ctggcgttaa accggggaga aagcgaaaaa atccttacag
     7621 taaaggtgat tcctcctgtg gatcagatca tgggatatct ggaaaagcag gtgatcgtaa
     7681 agaataatcc atataccact ccggtgttaa aggcggtggt gaaggacagc tatgaccgtc
     7741 tgacagggcc ttccatagag cgtgaaatcc gcagtgagat cacagaaaag gcagaggacg
     7801 gagcaatccg tgtttttgga aagaatttag agcagcttct gatgcagcct cccattacag
     7861 gccgtgtggt tttaggctgg gatccggcct tccgtacagg ctgcaagctg gcagttgtag
     7921 acagcacggg aaaggtgctg gataccattg taatctatcc cacagcgcct cagaataagg
     7981 tagaggaatc aaagaagatt ttaaaggaaa tgatccacaa atacaacatt tccctgatat
     8041 cggtaggaaa tggtacagct tccagagaat cggagcaggt aatagtggaa ctgttaaagg
     8101 aaatcaagga gccggtgaag tatgtgatcg taaatgaggc cggagcctcc gtatattctg
     8161 cgagcaaact tgccacagag gaatttccgg catttgatgt gggccagaga agtgcagtgt
     8221 ccattgcccg ccgcctgcag gatcctctgg cggaactggt aaagatcgat cccaaatcca
     8281 ttggcgtagg ccagtatcag catgatatga atcagaaaaa gctgggagct gccttagagg
     8341 gtgtggtgga agactgtgta aacagggtag gtgtggacat aaataccgca tctgcctccc
     8401 ttttggaata cgtttcaggc atcaataaga cgctggcaaa aaacattgtg gcgtaccgtg
     8461 aggaaaacgg cgcatttaag agcagaaagc agcttttaaa ggtagccaag ctggggccta
     8521 aggcttttga acagtgcgca ggctttatgc gtattacagg aggagcggaa cccttagacg
     8581 gtaccagtgt ccatccggaa agctatggcg cagctaaggc cattttacag agccttggat
     8641 acagggaaga ggaactggcg cgtggaggaa ttcccggtat tagccgaaaa atccgtgact
     8701 ataagaaaat ggcagaggaa gtgggagttg gagaaatcac gctcagagac attgcgggcg
     8761 agctggaaaa accggccaga gatcccagag atgagatgcc aaggccggtt ctgcgcagcg
     8821 atattctgga aatgaaggat ttaaaagccg gcatggtatt aaagggaaca gtacgcaatg
     8881 taattgattt tggagccttt gtggatatcg gtgtccatca ggacgggctg gtgcacattt
     8941 cacagatgac agaccgctac atccgtcacc ctatggacgc agtgagcgta ggagatgtgg
     9001 ttgatgtaaa gattctggca gtggatctgg agaaaaaacg gatttctctc accatgaaag
     9061 gggtaaagga tggtaattga aacatttgat ccaaaggaaa cctatgatct tggcgtgaaa
     9121 atgggagagc aggcccgggc cggacaggta atctgcttaa acggtgacct gggtgtggga
     9181 aaaactgtgt ttactcaggg gtttgcagcc ggtcttggca tcgcagggcc tgtaaacagc
     9241 cctacattta ccattgtgca gcagtatgag gacgggcgtc tgccgctgta tcattttgac
     9301 gtatatcgga ttggtgatat cagcgaaatg gatgagatcg gctatgaaga ctgcttttac
     9361 ggtcagggag tgacactgat cgagtggtcg gagctgatcc gggaaatcct gccggaacat
     9421 gtgacggaag tgaccataga aaaggatatg gaaaaaggat tcgactacag acgaatccgt
     9481 gtggaggaga aagaatgaga gtgttaggaa tcgaaagttc atctctggta gccagcgtgg
     9541 ctcttgtgac cgatgatatt cttacaggag aatatacggc caactttaaa aaaacccatt
     9601 ctcagactct gcttcccatg ctggatgagc tggcaaagct tctggagctg gatcttaata
     9661 ccgttgacgc cattgctgtg gcaggaggac cgggttcatt tacaggctta agaattggtg
     9721 cggccactgc caagggcctt ggactggcat taaaaaagcc tctgatccat gttcctaccg
     9781 tcgatgctct ggcatataat atgtggggag cctctggcct gatctgcccg attatggatg
     9841 ccagacgccg tcaggtctat acgggtctgt atcagatgga acaggggatt catgttcaga
     9901 tggaacagca gcctatggat atgggagatc tggcggaaga attaaaccgc aggggagaac
     9961 gtgtgatttt tctgggagac ggagttccgg cctataggga aatgatacag gaaatggtaa
    10021 aggttccctt tgaatttgcc ccggcccaga tgaaccgcca gagggcagcc agtgtggcag
    10081 cgcttggaag ccttgctctg aaggaagagg gcgacggctg cggataccat agtgcgtttt
    10141 tagtgtctgc cggagaattc aatcccgatt atctgagaaa atcacaggca gaacgccaga
    10201 gggaggctga actgtcgcca gagggcgctg ccggaaagct tccgggagat tattaaaaat
    10261 atcatttctg ctagcaaagg agtcgttatg aatcagaacc tgagggtacg ccggatggaa
    10321 gccggagacc ttttacgggc agcggcactg gagcagagca gtttttctca gccctggtct
    10381 gaaaagctgc ttgcagaggg cttaaacagt cctctggata catattttgt atgtacctgt
    10441 gaaaatgaaa tcgcgggcta ctgcgttctg cgtgtcctgg cagatgaggg agaactgcag
    10501 aggattgcgg tgttttcaga ataccgcagg cagggagtcg gcaggaaact gatggaagcc
    10561 atgttagcag ccgcaaggga agagggcgca tcctctgttt ccctggaggt cagagaaagt
    10621 aatgtaggag ccatgcgtct ttacgaagaa tacggtttct gtcaggaggc agtccgcaaa
    10681 aagtattacc aggagccgga ggaagatgca gtgatcatgt ggaaccgcag tttgtgactg
    10741 gtcttttgga attgcggagg aaaatataca gcaattccca cttaaaatta cagaatcctg
    10801 tgtatataat ggagaggaat cgaacattcc tctccgtttt gcgtattatg gccggaagaa
    10861 ggaagcgagg atcatgaaag agaaaggcga ggtatattga tgaaaaagta caataagaat
    10921 ggacagttag agacactgat ctgtaacggc tgcgggaaga aactggcagt gagccaaggg
    10981 attatacgag agggagccat atccatagac catgtgtggg attatttttc cgaaaaggac
    11041 gggcaggttc atcattttga tctgtgtgag gaatgttatg accagatgat aaataggttt
    11101 cggattcctg tttccataga ggaacagaca gaatatctgt aaaaggaagc cggacgagcg
    11161 tttttctcag ggctcttgat tattgggctg catctgtgtt atgatttgaa aaagccggtt
    11221 ttgcggaacg gtgaaaatgt tccgaaacgg tgcgacttga catagaaaat gaggaaaata
    11281 atatgcttga tatatgtttg ctgggaacgg gggggatgat gcccctgccc tatcgatggc
    11341 ttacatccat gatggccaga tgcggcggca gtaatcttct tattgactgc ggagagggaa
    11401 cacaggttgc gttaaaagaa aagggctgga gccctaaacc cattgatact atctgcttta
    11461 cacattacca tgcggatcat atcagtggtc ttccgggtct gcttttaact atgggtaacg
    11521 cagagcgtac agagcctctg accctgattg gccccagagg actggaaaga gtagtaaatg
    11581 cactgcgtat gatagctcca gagcttccct ttgagcttcg gatgattgaa ctgacagaaa
    11641 acaggcagca gcttacagcc ggcccttata tcattgatgc atataaggtt aaccacaatg
    11701 ttctctgcta tgggtatacg attcgcattc ccagaaaagg ccgttttgat gtagaacgtg
    11761 caaaggagca gcagatccca cagaaattct ggaaccgtct tcagaagggc gaagagattg
    11821 agtgcgaagg ccgcctgctt acaccggata tggttctggg gcccgaccgc aggggactca
    11881 aggtaacgta ctgtacggat acgcgcccta ttcctgtgat tgcagagtac gccgccggtt
    11941 cagatctctt tatctgtgag ggcatgtacg gagaaaagga aaaggcttcc aaagccagag
    12001 aatataagca catgaccttt tatgaggcag ctgctctggc gaaagaggcg aaaccgggac
    12061 agctctggct gacccattac agcccatctc ttacccgtcc ggatgattat atggacgagg
    12121 tccgcaagat ttttccgcgg gctaaggctg caagagacgg gtggaccatg gaactggaat
    12181 ttgatgagga gtgaaaaatg aacgtgaatc atacagaaga tgtactgata cttgcaattg
    12241 agagttcctg cgatgaaacg gcagcttccg tggtaaaaaa cgggcgtaca gtgctgtcca
    12301 atgtgatttc ctctcagata gcaacccata cggtatatgg gggtgtggtg ccggagattg
    12361 cctccagaga acatattaag gcaattaatt atgtgataga gcgggcattg gcagaggcag
    12421 aaacaacact ggatgatatt acagcaattg gagtgacata tggtccaggt ctggtggggg
    12481 ctcttttggt aggagtggca gaggctaaag ctattgctta tgcagcccga aagccactta
    12541 taggtgttca ccatattgaa ggccatgtat cggccaattt tattgaaaat aaagatctgg
    12601 aaccaccatt tatctgtctg attgtgtcag gaggacatac ccatcttgta gttgtaaagg
    12661 attatgggga atttgaaatt attggccata ccagagatga tgctgctggg gaagcttttg
    12721 ataaagtggc ccgtgcagtg ggactggggt atcctggtgg cccaaagatt gataaggcag
    12781 caaaagaagg aaatccccat gctattgaat tcccaagggg taaagtggca gattcaccct
    12841 atgattttag cttcagtggt ttaaagtcgg ctgttttgaa tcacatcaat catgcaaaaa
    12901 tgacgggtga agagatttgt gtaccggatc ttgtggcatc tttccagaat tcggtagtgg
    12961 attccctggt aagccgtgct attcatgcgg caaaggaata cggatacagc aagcttgcta
    13021 ttgcaggagg agtggcatca aatacagcac ttcgcgcagc aatgaaaaag gcctgtgaag
    13081 agaatggaat tgcattttat catccatctc ctgttttttg tacagataat gcggctatga
    13141 tcggtgcggc tgcttattat gaatatattc gaggaaacag atctggctgg gatcttaatg
    13201 ccgtacccaa tttgaaactg ggggagagat agatgaaaaa attctgtaca gcagttgtac
    13261 tggcggcagg aagaggcagc cgaatgggaa ctgcagtggc gaagcagtat ctgacattgg
    13321 gagataagcc tgttgtggtc tattcgttag aagcctttca ggcatctcct gttatagatg
    13381 agattattct tattactgat aaagaccata ttgaatattg ctgggaaaaa ttggttcatc
    13441 catatgggat taccaaggta acaacagttg ctccgggagg aaaggaacgc tatgaaagtg
    13501 tatggaaggc tctctgtctt ctttgcggga aaaattcgcg gaatgaagct ggggcttcgg
    13561 ccggagacgg ttatgttttc attcatgacg gggccaggcc ttttattacg gaggatataa
    13621 ttgagagagt atataaggat acagaaaaat gggatgcctg tgtagtggga atgccggtta
    13681 aggataccat taagcagacg gatgaaaatg gtgtgattct ggatactccg gatcgcagca
    13741 gaatctggca ggcgcagaca cctcaggttt tttcggttcc ccttattgtg gaggcatttc
    13801 agcggcagct gtcccaggac tgcagacgtg ttacggatga cagtatgata gtggaaaacc
    13861 agatgggtgt taaggtgcat atgactgaag gcggctatac caatattaag attacaaccc
    13921 cggaagacct ggctgttgca gaggctattt tgactcaata taaaaaatga aaaataattg
    13981 taaaaaaagt gttgacatct gtcgaatggc gtggtattat aactgagctg tcgctgatac
    14041 ggcagatgca acacacaaga gaaaataaac ttttgaaaaa atcaaaaaaa ctcttgacaa
    14101 aatgcttttc atcttgtaaa atagaaaagc acgtttggag agatatcgaa gtggtcataa
    14161 cgaggcggtc ttgaaaaccg tttgtccgaa agggcgcgtg ggttcgaatc ccactctctc
    14221 cgcttgggac aaacattgag atttgtcttg aacagaataa agattaaaag atcaaccaag
    14281 cagaagtacc caagtggttg aaggggctcc cctggaaagg gagtaggtcg ttagtagcgg
    14341 cgcgagggtt caaatccctc cttctgcgtt ggcaacgcaa gttgcaagta ccttgagaat
    14401 taaagactga ataatacaca aaccctgaaa attctaacaa ttcaaaaggt ttggtttaga
    14461 ccttaggatt tgagaaaatt cagaacaaaa accaagtaaa acggtttaag ataacattag
//
LOCUS       DBMDUH010000056        14185 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_2322_length_14185_cov_6.179565, whole genome shotgun sequence.
ACCESSION   DBMDUH010000056 DBMDUH010000000
VERSION     DBMDUH010000056.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 14185)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14185)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14185
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2322_length_14185_cov_6.179565"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="ACZPCG_06405"
     CDS             complement(<1..286)
                     /locus_tag="ACZPCG_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HIR1284733.1"
                     /translation="MAEILMKAAAFVAIIFMGYLLRKKGFFKEEDFHVLSKIVLKITL
                     PAAIVANFSGVDLQASLLILSVLGLGGGLMLITSAYLAAGKKREEKAFFML"
     gene            complement(403..1710)
                     /locus_tag="ACZPCG_06410"
     CDS             complement(403..1710)
                     /locus_tag="ACZPCG_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR1284734.1"
                     /translation="MTIAVACGLIILVLLVIMLLAGVPIAVALAVSSIGAILPVLDTG
                     ASVLTGAQRIFSGISVFSLLAIPFFILAGNLMNKGGIAIRLINFAKLFTGSIPGALAH
                     TNAVANMLFGAISGSGTAAASAMGSIIGPIEEEEGYDRDFSAAANIATAPTGLLIPPS
                     NVMITYSLVSGGTSVAALFMAGYIPGILWGLACMIVIYIFAKKKGYRSTKKFTNSEKL
                     KVFLQAIPCLFMIVVVIGGIISGIFTATEGSVVAVIYSMVLSVFFYKSIKPSELPKIF
                     LDSAEMTGIIIFLIGVSSIMSWVMAFTGIPTAVSEAMLGISDNRYVILVIINILLLIV
                     GTFMDMTPACLIFTPIFLPICQTLGMNTIHFGIMMIFNLCIGTITPPVGTTLFVGVKV
                     GQTKIEQVIKPLLAYFAAIFIVLMLVSYIPALSMWLPGLLGYV"
     gene            complement(1707..2210)
                     /locus_tag="ACZPCG_06415"
     CDS             complement(1707..2210)
                     /locus_tag="ACZPCG_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIR1284735.1"
                     /translation="METMHKVKAGIMQVLGFVIIALFAFMTLIGTYQIVTRYFFNRPS
                     TVSEELLTYAFTWMALLASAYVFGKRDHMRMGFLADKLIGAPRKYLEITIDLLTFLLA
                     AVVMVYGGISITKLTMIQKTASLQVPMAYVYVIVPVTGVLIMIFSLMNAIDVAHMEFK
                     EKGEVRL"
     gene            complement(2212..3261)
                     /locus_tag="ACZPCG_06420"
     CDS             complement(2212..3261)
                     /locus_tag="ACZPCG_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR1284736.1"
                     /translation="MCIKKGSSKVRKMMKKLTALAFGIAVAVSLTGCGSLTGGKRIIR
                     VSHAQSETHPEHLGLLAFKEYVEEKLGDKYEVQIFPNELLGSAQKAIELTQTGAIDFV
                     VAGTANLETFADVYEIFSMPYLFDSEEVYKSVMQDTEYMENVYASTDEAGFRVVTWYN
                     AGTRNFYGKSPIRTPEDLKGKKIRVQQSPASVEMVKAFGAAAAPMGFGEVYTAIQQGV
                     IDGAENNELALTNNKHGEVAKYYSYNKHQMVPDMLVANLKFLNSLSPEDYQVFKEAAA
                     LSTEVEMEEWDKSIEEAKKIATDEMGVEFIDVDVEAFKEKVLPLHESMLQENEKIRDL
                     YDHIQAANEKAKGGQ"
     gene            complement(3336..4106)
                     /locus_tag="ACZPCG_06425"
     CDS             complement(3336..4106)
                     /locus_tag="ACZPCG_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006783012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1284737.1"
                     /translation="MALAKRLHKHFGDSLVIWQAADGNEAVRLCFKERCQIVIMDISM
                     PELNGVEAAEQIRRLDEDCEIIFLTAYDEFSYAKRAIAIHALDYLLKPCEEEELTAVM
                     EEAVGRIQKREQFEKLHMEQEMPEETGAEMPEPDLEEEPDEPDRLGQAAEKIRRYIRK
                     NYMKDISMQDAARMMNYSDAYFCKLFKQCFDQNFTAYLARFRINEARRLLQDKNASVR
                     EVSMLVGYYDANYFAKVFKRIAGMNPSEYRDSFHTDSV"
     gene            complement(4172..5611)
                     /locus_tag="ACZPCG_06430"
     CDS             complement(4172..5611)
                     /locus_tag="ACZPCG_06430"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1284738.1"
                     /translation="MEKSGVSLEKKVKRIAVCAGAVVFFSIGANLAVSWFGLGSFGSI
                     LEQNAGSLAFWSAMEEERLAFARYVGTALPEERERFEQSRMETRRRLEELPVDYRETG
                     SERYARTWSIRNMYENYETELEVLEETDREDPEYVNRLYRIYRVQNYLKDKAGQLEQM
                     TMEDGSERYEKHRFLFSVLPAASILWGITALWMVKRLNGSVRRNIVEPVVMLAGQADR
                     IRKNDFSGPELTAEGEDEISRLIRSFNKMKQATRGYIEALKEKNETERQLEAVRLQLL
                     KNQINPHFLFNTLNMIAGTAQIEDAAATEKMIQALSRLFRYNLKSTASVMPLERELKV
                     VQDYMYLQQMRFGKRVQYETGCDPETMEILVPSFMLQPLVENAVKHGISARSKGGRIV
                     VRTWKQGQRLWISVADTGEGIDEDKLEKIKRALKEGDGSGVGIGLGNIARRLKAMYED
                     GEVRIESRKGCATVVQLAFTPENCKEDEP"
     gene            complement(5741..5959)
                     /locus_tag="ACZPCG_06435"
     CDS             complement(5741..5959)
                     /locus_tag="ACZPCG_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1284739.1"
                     /translation="MIDYSPFWETLEASEESWYTLTKKHKISDSTLHRLKHNKDVTMK
                     TINDLCRILNCRIEDIARYVPSEEDQIL"
     gene            6135..6335
                     /locus_tag="ACZPCG_06440"
     CDS             6135..6335
                     /locus_tag="ACZPCG_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284740.1"
                     /translation="MGIYTQKEKELLADDPEVRKRLLSFASMSGKKFFREVQSRLSPE
                     ELEEYLEENPEERIYLMTPPHK"
     gene            complement(6355..7062)
                     /locus_tag="ACZPCG_06445"
     CDS             complement(6355..7062)
                     /locus_tag="ACZPCG_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008718242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1284741.1"
                     /translation="MGEVNFKNRGELVFETLKREILDLKLKPGCLISESDICERFGVS
                     RTPVRDALRLLQEQGFAETIPYRGTYVTLLSLDNIKQMIYMRVAVETMVLRDFMEIQT
                     PMVMEEIRHAIAVQKALIQTPGFEPEQFYRMDAQMHSLWFTAVKRQKLWDMLQAQQLH
                     YTRFRMLDFITETDFPRIIGEHEQLFELICKKDLSGLEQVLKDHLYYSMKRMRHSIEV
                     DYKDYFEEEPEENRFVI"
     gene            complement(7162..7968)
                     /locus_tag="ACZPCG_06450"
     CDS             complement(7162..7968)
                     /locus_tag="ACZPCG_06450"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002596465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HIR1284742.1"
                     /translation="MADYLNNFSLEGKVALVTGASYGIGFAIAKAMAGAGATIVFNDI
                     KQELVDKGIAAYKEEGIEAHGYVCDVTNEDAVNAMVAQIEAEVGVVDILVNNAGIIKR
                     IPMCEMSAAEFRQVIDVDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGEYNIQCNGIGPGYIATPQTAPLREIQPDGSRHPFDQFI
                     VSKTPAGRWGEAEDLGGPAVFLASDASNFVNGLVLYVDGGILAYIGKQPK"
     gene            complement(8013..8855)
                     /gene="kduI"
                     /locus_tag="ACZPCG_06455"
     CDS             complement(8013..8855)
                     /gene="kduI"
                     /locus_tag="ACZPCG_06455"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HIR1284743.1"
                     /translation="MDIRYSANQRDVKRYTTEELRNEFLITDLYKANEVVAVYSHVDR
                     MVTLGCMPTTEKVSIDKGIDCWKNFGTHYFLERREIGIFNIGGEGTITADGQTFKMGY
                     KDCLYITKGTKEVYFESTDAEKPAKFYMVSAPAHTSYTTTFIPIEKAAKRPCGEAETS
                     NKRTINQFIHPDVLKTCQLSMGMTVLEPGSVWNTMPSHTHERRMEIYMYFEVPGDNVV
                     FHMMGEPTETRHIVMKNEEAVISPSWSIHSGAGTSNYTFIWAMGGENMEFDDMDTMMP
                     NEMM"
     gene            9108..10346
                     /locus_tag="ACZPCG_06460"
     CDS             9108..10346
                     /locus_tag="ACZPCG_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003503423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR1284744.1"
                     /translation="MKKNILVGQSGGPTAVINASLYGVIQEGLSHPDEIGAVYGMING
                     IEGFLSGHTINLSAELSKEDLELLRLTPAAYLGSCRYKLPENMDASVYPLLFEKFKAM
                     NIGAVFYIGGNDSMDTADKLSRYAAMAKEDIVFIGVPKTIDNDLPVTDHTPGYGSAAR
                     YVASTVREIVLDASVYQQPAVTIIELMGRHAGWVTAASALARKFPGDNPLLIYLPESD
                     FDMEAFLNDIEGALKKSTAVVVCVSEGIHDASGRLVCEYSTEAQVDTFGHKMLTGCGK
                     LLESYVRGRFGVKVRSVELNVNQRCSSLISSLTDINEASEAGRFAVRAALEGVTAKMI
                     TFEREEGADYSIRFGLTDVSNVCNKEKTFPPHWITGNHNDIGPEFTAYALPLIQGQPQ
                     HILEDGLPRYLYRKEQGLLP"
     gene            10343..11287
                     /locus_tag="ACZPCG_06465"
     CDS             10343..11287
                     /locus_tag="ACZPCG_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002600875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR1284745.1"
                     /translation="MIRFPTYESCTLCPRMCRIDRSRKTGYCGCTDQLKAARAALHKW
                     EEPCLSGPEWARGGSGTVFFSGCTLKCCFCQNYPISSEQFGKELTSNELADIFLRLED
                     QGAYNINLVTPTQFLPSILPALDQVRSRLSIPIVYNCGGYERPEIVEYLKDYVDIWLP
                     DLKYHDSALSARLSNCGNYFEAASRAIYQMILQTGAPVFEEHAPEEPSQPSFLLMKKG
                     VILRHMVLPGHRDDSIALMEWVSRELPKGQYLLSLLSQYTPFYHSCDHPDINRRITSY
                     EYQKVVDAALSLGLTEGFIQEKSSAKEEYTPPFDLKGL"
     gene            complement(12102..13454)
                     /locus_tag="ACZPCG_06470"
     CDS             complement(12102..13454)
                     /locus_tag="ACZPCG_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1284746.1"
                     /translation="MKKSYEIDMCNGPLLGKILLFSIPLMLSGILQLLFNAADIIVVG
                     RFSGSTALAAVGSTSSLINLLINIFVGLSVGVNVLVARYYGGQKERDVQETIHTAVLT
                     AILSGLFLVAVGELAAGPMLRIMGTPEDVLDQSVLYMRIYFLGMPVLMLYNFGAAILR
                     AVGDTRRPLYFLFAAGAVNVVLNLFFVIGLGMGVDGVAWATVISEHISAFLVFKSLMA
                     EEGMMKLHLKKLTIHMDKLKRIVKIGLPAGMQGAIFSVSNVLIQSSVNSFGSVAMAGN
                     TASANIEGFVYTAMNAVYQTSLSFTSQNLGGGKYSRINKVKNICLAVVTAVGLVLGLA
                     AVGAGTHLLGIYSSDSEVLKYGMLRLEIICGTYFICGLMDCMVGSLRGLGYSVIPMIV
                     SLTGACGFRILWIFTVFAVYRSLAVLYLSYPVSWLITAAAHMVTFKLIRRKFPKEDIK
                     "
     gene            13590..14153
                     /locus_tag="ACZPCG_06475"
     CDS             13590..14153
                     /locus_tag="ACZPCG_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004611407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071421 - manganese ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HIR1284747.1"
                     /translation="MSLTELFIIAVGLSMDAFAVAVCKGLAMPEMSWKNAGIVGLYFG
                     GFQGLMPFLGYLLGSKFSGTIESYDHWIAFVLLALIGGNMIKEAFENGEEAADPSLDI
                     KTMTLMAVATSIDALAVGVTFAFLQVRILPAVLFIGSITFLLSAAGVKIGNVFGCRYQ
                     KKAEFAGGLILILMGLKILLEHLGFLG"
BASE COUNT         3638 a         3693 c         2939 g         3915 t
ORIGIN      
        1 tcaacatgaa aaatgctttt tcctcacgtt tttttcccgc tgccagatag gctgaggtta
       61 taagcatcag gcctcccccc agaccaagaa ccgacaggat caaaagagac gcctgaagat
      121 caacccctga aaaattggct acgatcgcag ccggaagagt tatcttcaaa acgatctttg
      181 aaagcacatg aaaatcctct tctttaaaaa atcctttttt tctcagcaga tatcccataa
      241 aaatgatcgc aacgaaggca gccgccttca tcagtatttc agccatgatg attctcctta
      301 cactttttca ttccattgtg tactatagca gaaaccagcg ctgtcttcaa taccgtttcc
      361 gaaaaaatac gccccgccag cccggcggag cgcacatttc atttatacat atcccaaaag
      421 tcccggcagc cacatggaca aggcgggaat ataggatacc agcatcagca caatgaagat
      481 tgccgcaaag taagctaaaa gcggctttat cacctgctca atcttagtct gtcccacttt
      541 tacacccaca aacagagtgg ttcccacagg cggggtgatg gttccgatac acaggttaaa
      601 gatcatcata ataccaaagt gaatggtatt catacccaga gtctggcaga tgggcaggaa
      661 aataggagtg aagatcaggc aggccggcgt catatccata aaggttccca cgatcagaag
      721 gaggatattg atgataacga ggatcacata ccggttgtcg ctgattccca gcattgcctc
      781 ggatacagca gtgggaatgc ctgtaaatgc cataacccag ctcatgatgc ttgacacgcc
      841 gataagaaaa atgataatac cagtcatctc cgcgctgtca aggaagattt tcggcagctc
      901 agagggtttg atggacttat agaagaatac agacaacacc atgctgtata taactgccac
      961 cacgcttcct tctgtagcgg taaagatacc ggaaatgatc ccgccgatta ccacaacgat
     1021 cataaacaga caagggatgg cctgtaaaaa taccttaagc ttctcgctgt ttgtaaactt
     1081 ctttgtactg cggtagccct tctttttagc aaaaatatag attacgatca tacatgccag
     1141 tccccagagg atcccgggaa tatagcccgc catgaaaaga gccgctacgg aggttcctcc
     1201 gctgacaagt gaataggtga tcataacgtt gctcggaggg attaaaagac cggtaggagc
     1261 ggtagcgata ttagccgctg ctgagaaatc cctgtcatag ccttcttcct cttcgatagg
     1321 gccgatgatc gaacccatgg cggaagctgc cgccgtaccg gaacctgaga tggcaccgaa
     1381 aagcatattg gcaacggcgt tggtgtgtgc cagagctccc ggaatacttc ctgtaaataa
     1441 ttttgcaaaa ttgatcagac ggatagcaat accgccctta ttcatcaagt ttcctgccag
     1501 gataaaaaac ggaatggcaa gaaggctgaa tacggaaata ccggaaaaaa tcctctgtgc
     1561 tcccgtaagc acggatgcgc cggtatcaag caccggaagg atcgctccga tggaagaaac
     1621 agccagagct actgcgattg gtactccagc taagagcata atcacaagaa gcactaaaat
     1681 aatcagcccg caggcaactg caatcgtcat aaccgaacct ctcccttctc cttaaattcc
     1741 atatgagcca cgtctatggc attcataagg ctgaaaatca tgatcagcac tcccgtaaca
     1801 ggcacgatca cataaacata ggccataggc acctgcaaag aagcagtttt ttggatcatg
     1861 gtcagctttg taatggaaat accgccgtat accatcacaa ccgctgctaa caaaaaagta
     1921 agaaggtcaa tggtaatctc cagatatttt ctgggcgctc cgatgagctt gtctgccaga
     1981 aagcccatac gcatatggtc tctctttccg aacacatagg cggaagccag aagggccatc
     2041 caggtaaaag cgtaggttaa aagctcctct gataccgtac ttggacggtt aaagaaataa
     2101 cgggttacga tctgatacgt tccgatcagg gtcataaagg cgaacagagc gatgattaca
     2161 aatcccagaa cctgcatgat ccctgctttt actttgtgca tggtctccat cctactgtcc
     2221 tccttttgct ttttcattgg cagcctgaat atggtcatag agatcgcgga tcttctcatt
     2281 ttcctgaagc atagactcat ggagaggaag aaccttttct ttaaacgctt ccacatccac
     2341 gtcaataaac tctacgccca tttcatctgt ggcgattttt ttagcctctt ctatactctt
     2401 gtcccactct tccatctcta cttccgtgga aagagccgcc gcctctttaa atacctgata
     2461 atcctcaggg cttaagctgt ttaagaactt cagattggct accagcatat ccggcaccat
     2521 ctgatgcttg ttataagaat aatattttgc aacttcgccg tgcttgttgt tggtaagagc
     2581 cagttcgtta ttctctgcac cgtcgatgac accctgctgg atagccgtat atacctcgcc
     2641 aaatcccatt ggcgccgctg ccgctccaaa tgcctttacc atctctacgc tggcaggact
     2701 ctgctgtacg cggatctttt tgccctttaa atcctccggc gtccgtatcg gggattttcc
     2761 gtagaaatta cgggtacctg cattgtacca ggttactacg cggaaaccgg cttcatccgt
     2821 cgatgcatag acattttcca tgtattccgt atcctgcatc acggatttgt atacctcctc
     2881 tgagtcaaac agatacggca tactgaaaat ctcatatacg tcagcaaagg tctccagatt
     2941 cgctgtgccg gctactacaa agtcaatggc tcccgtctgt gtcagctcaa tggccttctg
     3001 ggctgagcct aaaagttcat ttggaaatat ctgaacctca tatttgtctc ccagcttctc
     3061 ttccacatac tctttaaatg ccagaagccc cagatgctcc ggatgcgtct ctgactgtgc
     3121 atgggaaacg cggataatac gcttgccgcc ggttaaagaa ccgcagcctg tcagactgac
     3181 tgccactgct atcccaaaag caagcgctgt cagctttttc atcatcttcc tcactttact
     3241 gcttcctttc tttatacaca ttttattaaa taattataaa aaaaggattt tctgttttgt
     3301 accgtaaatc tttgacattg agggagaaaa ttttgtcata cgctgtctgt gtgaaagctg
     3361 tctctgtatt cagaagggtt catacctgca atccttttaa atacctttgc aaagtaattg
     3421 gcgtcataat accctaccag catgctgacc tctcttacgc tggcattttt atcctgtaaa
     3481 agccgtctgg cctcattgat cctgaagcgg gccagatagg cggtaaaatt ctggtcaaag
     3541 cactgtttaa acagcttgca gaaataggcg tcagaataat tcatcatccg tgccgcatcc
     3601 tgcatggaaa tatccttcat ataatttttc cgtatatacc ggcgtatctt ttcagccgcc
     3661 tgtccaagcc ggtcaggctc gtccggctcc tcctctaagt ccggctccgg catctctgcc
     3721 ccggtctctt ccggcatctc ctgttccata tgcagctttt caaactgctc cctcttttgg
     3781 atccgcccca cggcttcctc catcaccgca gtaagctcct cttcctcaca gggctttaaa
     3841 agatagtcca gcgcatgaat ggcaatggct ctctttgcat aggaaaattc gtcatatgcg
     3901 gtaaggaaaa tgatctcgca gtcctcatcc agccttcgga tctgttctgc cgcctccaca
     3961 ccgtttaatt ccggcatgga aatatccatg atcacgatct ggcaccgttc tttaaaacac
     4021 aggcggaccg cctcatttcc gtctgctgcc tgccagatga ccaggctgtc tccaaaatgc
     4081 ttatgcagcc gtttggcaag agccatacgt tcaatctgct catcatctgc caaaagcacc
     4141 ttgtacgtct cgttcttatc ctcattctgt atcacggttc atcctcctta cagttttccg
     4201 gtgtaaacgc cagctgtacc accgtcgcgc agccctttcg gctctcgatc ctcacttctc
     4261 cgtcctcata cattgccttc agccggcggg caatgtttcc cagaccaatc ccaacgccgg
     4321 atccatctcc ttcctttaag gctcttttta tcttttccag tttgtcctca tcaatccctt
     4381 ctcccgtatc cgccaccgag atccacagcc tctgcccctg tttccaggtc cgcaccacaa
     4441 tccttccgcc tttgcttctt gcagaaatcc catgctttac tgcgttttcc accaaaggct
     4501 ggagcataaa ggaaggaacc agtatctcca ttgtttccgg gtcacagcct gtctcatact
     4561 gcactctctt tccaaaccgc atctgctgca gatacatata atcctgaaca accttaagct
     4621 cacgttccag aggcatcaca gatgcagttg attttaaatt gtagcggaac aggcggctta
     4681 atgcctgtat catcttttcc gtggcagccg catcttcgat ctgagccgtt ccggcgatca
     4741 tatttaaggt attaaaaaga aaatggggat tgatctgatt ttttaaaagc tgcagccgga
     4801 ccgcctccag ctgccgttct gtttcgtttt tttcttttaa agcctcaata tatcctcttg
     4861 tcgcctgctt cattttatta aaggaacgga taagacgact gatctcatct tctccctcag
     4921 ctgtaagttc aggccctgaa aaatcatttt tccggatacg gtctgcctgc ccggccagca
     4981 ttaccactgg ctctacgatg ttacggcgta cggagccatt cagccgctta accatccaca
     5041 gtgcagtgat cccccacaga atactggcag ccggaaggac tgaaaaaagg aaacgatgct
     5101 tctcgtagcg ttcgcttccg tcctccattg tcatctgttc cagctgtccg gccttatcct
     5161 tgagataatt ttgaacccgg tagatccgat acagccggtt cacatattcc ggatcctccc
     5221 ggtctgtttc ttcaaggact tccagctctg tctcgtaatt ttcatacata ttccggatgg
     5281 accaggtacg ggcataccgc tcagagcccg tttctctgta atcaacggga agctcttcca
     5341 gccgccggcg cgtttccatt ctggactgct caaaccgctc ccgctcttcc ggaagggcag
     5401 ttcccacata acgcgcaaag gccaggcgct cctcctccat cgcagaccag aaggccagac
     5461 tgccggcgtt ctgttccagg atgcttccga agctcccaag gccgaaccag cttactgcca
     5521 gattggctcc aatcgaaaaa aagaccactg ccccggcaca gacagcgatc cgttttactt
     5581 ttttctccag cgaaactcca cttttttcca tatatttcct ccggatccgt tccttttaag
     5641 aagattgtcc acaaaatctc ctataagccc acatagtata tcacagctta tatccttttg
     5701 aacagacccg gaccgttcct tttatgtctt tttaacattc ctaaagaatc tgatcctcct
     5761 ctgagggcac atacctggcg atatcctcaa tgcggcagtt taaaatcctg cagagatcat
     5821 tgatcgtttt catggtcaca tctttattat gcttaagccg gtgcagagta ctgtcagaaa
     5881 ttttatgctt cttcgtcagt gtgtaccagc tttcctccga cgcctccaga gtttcccaga
     5941 aaggcgaata gtcgatcata tttttattgc tcctatgctt attttacctg aaataagcat
     6001 aagttagaat ttgcatattg aatatcttcg gaatagcgaa tatactatag gtaaacagat
     6061 gcatattctg aaattatcag actattctaa ccgttttgag tcaaaataag agacgggaaa
     6121 ggaagggatt ggatatggga atttatacac agaaggaaaa ggaattgctg gcagatgatc
     6181 cggaagtaag aaagcggctc ctaagctttg cctccatgag cggaaaaaaa tttttcagag
     6241 aggttcagtc ccgcctgtca ccggaggaac tggaagaata tctggaagaa aatccggaag
     6301 agcggatcta tcttatgact cccccccaca aataaaacaa aaaacggcct gccattaaat
     6361 gacaaaccga ttctcttctg gttcttcttc aaaatagtcc ttataatcca cctctataga
     6421 atgacgcatc cgcttcatac tgtagtagag gtgatctttt aatacctgtt ccaggccgga
     6481 aagatctttt ttgcagatca gctcaaacag ctgctcatgc tctccgataa tccttggaaa
     6541 atctgtttct gtaataaaat ccagcatacg aaaccgggta taatgaagct gctgggcctg
     6601 gagcatatcc cagagcttct gtcgctttac agctgtgaac cagagagagt gcatctgggc
     6661 gtccatgcgg taaaactgtt ccggttcaaa ccccggcgtc tggatcaagg ccttctgaac
     6721 ggcaatggca tgacggattt cctccatcac catgggcgtc tggatttcca taaaatccct
     6781 taaaaccatg gtttccaccg ctaccctcat atagatcatc tgcttgatat tatccaggct
     6841 caaaagagtc acatacgttc cccgataggg aatcgtctcc gcaaatccct gttcctgcaa
     6901 aagacgcagg gcgtcccgca ccggagtccg cgacacgcca aaccgctcac agatatcact
     6961 ttcgctgatc aggcagcctg gtttcagctt cagatccagt atctcccgct ttaaggtttc
     7021 aaaaaccagt tcccctctgt ttttaaaatt cacttctccc atgcagcacc cctgttcctt
     7081 ttctcagatg aatcaggacc cgccatgcgg tcatggcatg acgggccctg attggtttta
     7141 ttggtttaat tgcgtaatcg attacttagg ctgcttgccg atgtaagcca ggataccgcc
     7201 gtctacatac agtaccagac catttacaaa attggaagca tcagatgcca ggaatactgc
     7261 aggtccgccc agatcctcag cctcacccca gcgtcctgca ggagtcttgg aaacgatgaa
     7321 ctggtcaaat ggatgtctgg agccgtctgg ctggatctca cgcagaggag ctgtctgggg
     7381 agtagcgata tagccaggtc cgataccgtt acactggata ttgtactcac cgtactcaga
     7441 agcaatgttc tttgtcagca tctttaagcc gcccttagct gctgcataag cagatacggt
     7501 ctcacggccc agctcactca tcatggagca gatgttgatg atctttccgt ggcccttctt
     7561 gatcatgctt gggataacag ccttggaaac aataaacgga gcgtttaagt caacatcgat
     7621 tacctggcgg aactcagctg cactcatctc gcacattggg attctcttga tgattcctgc
     7681 gttatttacc aggatatcaa ctacgcctac ctctgcctcg atctgagcaa ccatcgcgtt
     7741 tactgcgtcc tcattggtta cgtcgcatac atagccgtga gcctcgatgc cctcttcctt
     7801 gtacgctgcg attcccttat ctaccagttc ctgcttgata tcgttgaaaa cgatggtagc
     7861 gcctgcgcct gccattgcct ttgcgattgc aaagccgatg ccgtaggaag cgcctgttac
     7921 cagggctacc ttgccttcaa gtgaaaagtt gtttaaataa tcagccattt ctttatcctc
     7981 catatatttt taacagaatc gttcggatga acttacatca tctcgtttgg catcatggta
     8041 tccatatcgt cgaactccat gttctctccg cccatagccc agataaaggt atagttggaa
     8101 gtgccggcgc cgctgtggat gctccaggac gggctgatca cagcctcctc attcttcatg
     8161 acaatatgtc ttgtctcagt gggttctccc atcatatgga atactacgtt gtcgcccgga
     8221 acctcaaagt acatataaat ctccatacgg cgctcatgtg tatgagacgg catggtgttc
     8281 catacgctgc ctggctctaa tacggtcatg cccatggaca gctggcaggt cttaagtaca
     8341 tccggatgga tgaactgatt gatggtacgc ttattggagg tctctgcctc gccgcagggt
     8401 ctctttgcag ccttctcaat cggaataaag gtagttgtgt aggaagtatg agccggtgcg
     8461 ctgaccatgt agaactttgc aggcttctca gcgtcggtgc tctcaaaata aacttccttt
     8521 gtgccctttg taatgtacag gcagtccttg tatcccatct taaaggtctg gccgtctgct
     8581 gtgatggttc cctcgccgcc gatattaaaa ataccgatct cacggcgctc taaaaagtaa
     8641 tgggtgccga agttcttcca gcagtcgatt cccttatcaa tagaaacctt ctctgtagta
     8701 ggcatacatc ccagagtaac catacggtct acatgagagt atacggcaac tacctcatta
     8761 gccttgtaca gatctgtgat gaggaattcg tttcttaatt cctctgttgt gtagcgcttt
     8821 acatccctct ggttggcaga atagcgaata tccattctcc gattcctcct ttaaaatatt
     8881 tgtatttaac tttttgagtt tgtatacaac ttttttatct gtatacagac tatcattttt
     8941 ttacctaaaa tgcaatagga aatatgttac aaaaaaatac gggttttata tgcaatatca
     9001 caaacggatt gcatttccca tttttatcag atatacttac aaaagaatct ggacttcttc
     9061 tgcagctgtc cagacgatca ctgtatacat gagaggagga ttttcccttg aagaaaaata
     9121 tacttgtagg ccagtccggc ggtcccacag ctgtgatcaa cgccagctta tacggcgtga
     9181 tccaggaagg actttcccat ccggatgaga ttggagctgt ctatggcatg ataaacggca
     9241 tcgaaggctt tctgtccggc cataccataa atctctccgc agagctttcc aaagaggatt
     9301 tggaacttct gcgcctgacg cccgccgcat atctgggctc ctgccgctat aagcttcctg
     9361 aaaatatgga tgcttccgta taccccttgt tatttgaaaa atttaaagcc atgaatatcg
     9421 gagctgtatt ctacatcggc ggaaatgatt ccatggacac cgcagacaaa ttgtccagat
     9481 atgctgccat ggcaaaagag gatatcgttt ttatcggcgt tccaaaaacc attgacaacg
     9541 atcttcctgt aaccgaccac acccccggct acggcagcgc tgcccgatat gtagcgtcta
     9601 ctgtccgtga aatcgttctg gatgcttctg tctatcagca gccggctgtc accattattg
     9661 aactcatggg ccgtcatgca ggctgggtca ctgctgccag cgccctggca agaaagtttc
     9721 ccggcgacaa tcccctgctc atctatctgc cggaatctga ttttgatatg gaagcgtttt
     9781 taaatgatat cgaaggcgct ttaaagaaaa gcaccgctgt ggttgtctgt gtttctgaag
     9841 gcatccacga tgcttccggc cgtctggtat gtgaatattc tactgaagct caggtcgata
     9901 cctttggcca caagatgctt acaggctgcg gaaagctgct cgaaagctat gtcagaggcc
     9961 gtttcggagt caaggttcgc tctgtagaat taaatgtaaa ccagcgctgc agcagcctta
    10021 tctcctctct tacggacatt aacgaagcca gcgaggccgg acgctttgca gtccgtgccg
    10081 cacttgaggg cgtgaccgca aagatgatca cctttgaacg cgaagaagga gcggattatt
    10141 ccatccgttt cggccttaca gatgtttcca atgtctgcaa taaggaaaag accttcccgc
    10201 ctcactggat caccggcaat cacaacgata tcggcccgga atttaccgcc tatgctcttc
    10261 cgctcatcca ggggcagcca cagcatattc tggaggacgg acttccaaga tatctgtacc
    10321 gcaaagagca ggggcttttg ccatgatccg ttttcccact tatgaatcct gtaccctctg
    10381 ccccagaatg tgccgcattg accgcagccg caaaaccggc tactgcggat gtaccgatca
    10441 gttaaaggct gccagggcag cactccataa atgggaagaa ccctgtctga gcgggccgga
    10501 gtgggccaga ggcggaagcg gaaccgtctt tttttccgga tgtaccctta aatgctgctt
    10561 ctgccagaat tatcccatca gcagcgaaca attcggaaag gagctgactt ctaatgagtt
    10621 ggctgatatc tttctccgtc tggaagatca gggggcctat aatatcaatc ttgtcacccc
    10681 cactcagttt ctcccctcaa tccttcctgc tctggatcag gttcgctccc gcctttcgat
    10741 cccgatcgta tataactgcg gcggctatga acgtcctgag atcgtagaat atttaaagga
    10801 ttatgtagat atctggcttc ctgatttaaa gtaccatgac agcgctcttt ctgcccgtct
    10861 gtcaaactgc gggaactact ttgaagccgc ttcccgtgct atctatcaga tgatccttca
    10921 gacaggcgct ccggtatttg aggaacacgc ccctgaggag ccctcccagc cttccttcct
    10981 acttatgaaa aaaggcgtaa tcctgcgcca tatggttctc cccggacaca gggatgattc
    11041 catcgccctg atggaatggg tcagcagaga gcttccaaaa ggccagtacc tgttaagcct
    11101 tttaagccag tacaccccct tctaccatag ctgcgaccat ccggatatca atcgtcggat
    11161 cacctcctat gaataccaga aggtagtgga tgcagcgctc tctctgggac ttactgaggg
    11221 atttatacag gaaaaaagca gcgcaaaaga agaatatacg ccgccctttg atctaaaagg
    11281 tctgtgatcc cctatattaa atacaacaaa agacggatat cgaatctttg actaaccacg
    11341 ttctcgatta cgatatccgt cttttaacta tctcgtccat tggaccaagc ctctctttcg
    11401 aaacaattgt ttcttttgtg actctatatt taattgtatc cactctggtt ttcttctgct
    11461 ctcttttgta tattcacttg cttcggatct attttccacc ttgcttttct ttctttgatc
    11521 cggtatctct ctccgtcctg atgatacttt ctcgtcatat caccagaccg ggcatttatt
    11581 gcctttgata cgcttatgga tcactttgaa aatacgatca tctgcttgtt cctatctttt
    11641 attacagaca ttctctttcg tttctttaag actgcaattc ttttaccaat cgattggact
    11701 ttcttttgtt tcttaaatca atttccgttt catctgtctg tactatgtat gagcagttac
    11761 tctcacagag tgctttccct gtttcatcta taactaaatc actttagctt ataaatgaaa
    11821 cgaacattct ctttctggtg gtccgtacct cctttgctta gattttatta cattgagttg
    11881 actgtctaca actccttatt ctcttttgta tctcgtgttc tcttctcttt tgttgacttt
    11941 attatacaaa aagagcccct atttgtcaag actctttttg taatttttta attatttttt
    12001 gtctttttat aaattgtgta tatttattgt aattgtttat atttttatat aatatttacc
    12061 tgttatattt gggtatttac ttataatttt cagaatattt attattttat atcttctttg
    12121 gggaatttcc tgcggatcag tttaaaggta accatgtgag ccgctgccgt aatgagccaa
    12181 gataccggat atgacagata aagaactgcc agtgaacggt aaacggcaaa tactgtaaaa
    12241 atccagagaa tacgaaaacc acatgctccg gtaagagaga cgatcatggg aatcaccgag
    12301 tatcccaatc ctctcaagct tcctaccata cagtccatca gcccgcagat aaaataggtc
    12361 ccacagataa tctccagacg gagcatcccg tatttaagca cctcagagtc tgaagaataa
    12421 attcccagaa gatgagtgcc cgcgcctact gctgccaggc caagaaccag ccccactgcc
    12481 gtaacaacag ccagacagat attttttacc ttattaatac ggctgtactt tcctccgcca
    12541 agattctggc tggtaaagct taagctggtc tgataaaccg cattcatagc cgtatagaca
    12601 aagccctcga tatttgcaga agccgtattg cctgccatgg ccacagaacc aaaggaattg
    12661 acagaggact ggatcagcac attggataca gagaagatcg cgccctgcat accggccgga
    12721 agaccgatct tcacaatccg cttcagctta tccatatgaa tggtaagttt ctttagatgg
    12781 agcttcatca ttccctcttc cgccatcagg ctcttaaaga caagaaatgc ggaaatgtgc
    12841 tcagaaatca cagtagccca ggctactccg tccactccca ttccaaggcc tattacaaag
    12901 aacagattca aaaccacatt gaccgctccg gcggcaaaga ggaaataaag aggccgcctg
    12961 gtatctccta ctgctctgag gatcgcagcg ccgaagttat aaagcattaa aacaggcatt
    13021 ccaaggaaat aaatccgcat atagagcaca gattgatcca gcacatcctc cggggttccc
    13081 atgatccgaa gcatggggcc tgcggcaagc tctcccaccg caaccagaaa caggccgctt
    13141 aaaatggccg tcaaaaccgc cgtatgtatg gtttcctgaa catctctttc cttctgtccg
    13201 ccataatatc tggctaccag cacatttaca cctacagaca agcctacaaa aatgttgatc
    13261 agaagattga tcagggatga ggtagagccc acagccgcca gcgccgtact tccggaaaac
    13321 cttcctacta caataatatc agccgcatta aacaaaagct gtaaaatacc ggaaagcatc
    13381 agcggaatgg aaaacagcag gatcttgcct aaaagcgggc cattgcacat atcaatttca
    13441 tatgattttt tcataatcct atttacctcc gtttcagcta tactttagca caggcaaatt
    13501 cgttttacaa gctcctatat ttttcttgac atttttcggt tcctctccta cactgtttca
    13561 taaactgtgc catttgggag gaaatgatca tgtcattaac ggaattgttt attattgcag
    13621 ttgggctgtc tatggacgcc tttgccgtag cggtctgtaa ggggcttgcc atgccggaaa
    13681 tgagctggaa aaacgccgga atcgtaggac tttattttgg cggctttcag ggccttatgc
    13741 cctttctggg atatctgctg ggttccaagt tcagcggaac catcgagtcc tatgaccact
    13801 ggatcgcctt tgtgcttctg gctcttatcg gaggaaatat gataaaggaa gcctttgaaa
    13861 acggcgaaga agcagcagat ccctctctgg atataaaaac catgaccctg atggctgtgg
    13921 caaccagcat tgatgccctg gccgtaggcg taacctttgc cttccttcag gtccggatcc
    13981 tgcctgccgt actctttatc ggaagcatta ccttccttct gtctgccgca ggtgttaaga
    14041 ttggaaatgt atttggctgc cgttatcaga aaaaagccga atttgccggc ggcctgatcc
    14101 tgatcctcat ggggcttaaa atccttttgg agcatctggg ctttctggga taaaacagag
    14161 atttaaaaaa gcatccggac attcc
//
LOCUS       DBMDUH010000057        14110 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_2332_length_14110_cov_4.880577, whole genome shotgun sequence.
ACCESSION   DBMDUH010000057 DBMDUH010000000
VERSION     DBMDUH010000057.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 14110)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14110)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14110
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2332_length_14110_cov_4.880577"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="ACZPCG_06480"
     CDS             <1..184
                     /locus_tag="ACZPCG_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284748.1"
                     /translation="QGDPELCYAHFALQRLHIRPGVLAGICGANDPVGPRERAFIYAS
                     IDLRVKEEKRQAAQLS"
     gene            198..2180
                     /locus_tag="ACZPCG_06485"
     CDS             198..2180
                     /locus_tag="ACZPCG_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002603336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HIR1284749.1"
                     /translation="MPTLNAMFRLMDGYSSQIKKIVENTDKAAKAILGASKKTDGFND
                     SLKRTGAAASVANSGLSKLVKTAMSLAAVKKGMDLTDTYTNTNARLGMITGSLEEQRA
                     LQKDVFAAAGRARGNYTEMANATAKLKMLAGDTFGSNQEAVGFTELLTKSLKISGAST
                     AEQNSAFLQLTQAMTAGKLQGDEFRSIMENAPMVADAIAQYMGKSKAELKELSSHGAI
                     TADIIKGAMFSAADEINGKFADMPPTFADTWQKIKNAGMQALGGVFEKANTVLNSAGV
                     QTALNNFIGLIYLAGEATEGFIDFCIAAWPMVSPFIWAAAAALGAYAAAQIVSNGLSL
                     ISATGFGAQAIGAGIYALALWATTGATWAETTAMLGLNSSLYACPLVWIVGLVLVLTA
                     AFYAGVAAVNHFAGTSISATGLIGGAFFALFAFVYNHFVYPIIAVFSMLANFIGNTCF
                     NDATAAVKVLFLDMANACIGYVLNMARSIENIVNKIPGVTVDITSGLEGLQTGLETKI
                     TAIKDESGWKEYVKQPELLDYSEMASKGYNAGKNLADKASNLFSGFNPSLDGAGGSGI
                     DFSSFATAGNPATVKGTGKGGAVKVETEKEDIEWMRKLAERDYVARIAQNTLAPNIRV
                     EFSGPITKEADTDGLVGHVVDRLKEVIATAPEGVPE"
     gene            2180..2842
                     /locus_tag="ACZPCG_06490"
     CDS             2180..2842
                     /locus_tag="ACZPCG_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002603337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR1284750.1"
                     /translation="MSYSVYFKYGGKKYKLPVNPEEIKRSRELNIENYQVLGTGQVSV
                     PSYYSLEEFSFEAEFPSQDYHYMNSGSRADADYYEKMFRKAQKNMKPVRFIASNDITD
                     DISVMVLVKSVEAVEKAGEEGDKYLSIKLQEYKAPGKRYVAVQTVAATVKQEDTAAVA
                     ETNPAVTENKTHTVQSGDTLWGIAKKYYGNGSQYTKIASANPDIKNPNLIYPGQVLSI
                     PV"
     gene            2854..3822
                     /locus_tag="ACZPCG_06495"
     CDS             2854..3822
                     /locus_tag="ACZPCG_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdQ/YqbQ family protein"
                     /protein_id="HIR1284751.1"
                     /translation="MEVLVESQGYIYDISEMCKEISWTEALNEGASCMDISYISDGLI
                     LQNGDAIRLTDNDQTDGIFFGAAYKVSGDSGDGGTGQIVTIKAYDQLRRAKNKDIIVL
                     ENGTLKNLVENMCTFQTMTPGTVEDPGFIIPTIADYEKSWLDHIVQAVSDTLIGTQEH
                     YCLRDEYGKVCLWNMRNLQLPLVLGDNSLCTGYSWEKSIDDDYYNQIKVVWKNEDTGQ
                     IDVGAAHDQEAVNRYGLLQYLESSPSGVDNAAKAQERANNLLKLYNHEKETLKLECLG
                     DLRVRAGNSVYGSIADIALNRRLIVKKVTHDFLPVHTMTMEVMAGE"
     gene            3815..4096
                     /locus_tag="ACZPCG_06500"
     CDS             3815..4096
                     /locus_tag="ACZPCG_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
                     /protein_id="HIR1284752.1"
                     /translation="MNDRGSVQELFNLIKTVVDNYMKSRKPAAVLVGTYTGNGVMIEK
                     LPIPMSMITGNAKSQLVSGDKVRLLRNDRGHEYYILEIIGKPYQTAGGT"
     gene            4096..4482
                     /locus_tag="ACZPCG_06505"
     CDS             4096..4482
                     /locus_tag="ACZPCG_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HIR1284753.1"
                     /translation="MAELTTDLIVQEQTYKARTYGLSETKIEGFVDELAALKQAIYKI
                     LSTEQYEYPIYSFKYGIAWKELIGEERAYVRAEMRRMIEEALLQDDRIREVDGFRFEF
                     SGDSCHCSFQVSSIYGEIQIEKEVAV"
     gene            4479..5507
                     /locus_tag="ACZPCG_06510"
     CDS             4479..5507
                     /locus_tag="ACZPCG_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HIR1284754.1"
                     /translation="MKMTYEELLQAMLDRVPSDVDKREGSVIYDALAPCAYFLAQQDF
                     QLDNFIDLVFPDTALGEYLDRAAEAYNVSRKPATKAMRMMTTSGRVEIGTRWGINNLV
                     YRVKEEESETEYRVECETPGVIGNQYSGAMQPISNITGITAELGDIITPGADEETDEA
                     LRERLYTKIRLPATSGNVYHYQQWALEVSGTGAAKVFSLADGPGTVTVLVVDSDKKIS
                     SSLPETVAAYIETMRPIGASVTVKSPEAVTINVKAKIMRDGSKTLEDIQAAYKLAVDS
                     FLRETVFATYRISHAKLGSLLLDIPGVEDFEGFLLNGGTGNVTVGEKQIPVTGTIELT
                     EVSRIGTD"
     gene            5494..6048
                     /locus_tag="ACZPCG_06515"
     CDS             5494..6048
                     /locus_tag="ACZPCG_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HIR1284755.1"
                     /translation="MELIKLLPDYYEKNVTMQTLQGLLSEVTDDLEQGLSSTISECFA
                     STASELLARYEQLLGLEVDVSKPDDFRQERIRAKISGVGTTTKEMVKNVASSYSNGEV
                     EVIEDAAHYRFVIRFVGILGIPGNMADLKLTIEEIKPAHLVVEYEYIYNVWSDVEMLT
                     WEQAAARTWEEIRSNRINGINRQI"
     gene            6026..6433
                     /locus_tag="ACZPCG_06520"
     CDS             6026..6433
                     /locus_tag="ACZPCG_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284756.1"
                     /translation="MELTDKYKLKKPGASDYINVEDFNENAEIVDREMEKHENLLDRL
                     YAAPIRITLTASGWAGSSVPYTQTVAVPSIKTEDNPVLVSQLADGATIEVQKAYSKAF
                     GIVASGTGQTGDGTVTFKVYKKPAIDIVVGLKL"
     gene            6449..7732
                     /locus_tag="ACZPCG_06525"
     CDS             6449..7732
                     /locus_tag="ACZPCG_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-rich domain-containing protein"
                     /protein_id="HIR1284757.1"
                     /translation="MGNILMTGSGGGGAGSDDCTATAAELLKGYTGILKGSDDEPVQG
                     ILELTGNAQAAHVLNGETFYSNDAKTKHTGNMTVNSLLSFSVAAYSGRRVIATWTNPN
                     QAAGRPYSGVIIRYDTGGYPGASGGVQIYKGAGNNHSAGAASQAFLDLPNLNTRYFLS
                     CTPYVTTSIGELFGPTLNAEITTGGSTSRTFTASGSFVVEGFTKMDVFLVGGGGGAGY
                     AGGGSGYTATQYGIPITTGNNVSVVVGGGGGPASNGGTSSISVNGAVKASAAGGNGAP
                     DSYGVAAGNGGSGGGGSSTGRVRGGNGGSDGGNGAFPKFPNGTGQGRTTRAWGNSVGA
                     LYAGGGAGGNNHYNNRNNEGDRVEYFNLGGTGGAGGGGNGGGWEGTNYGKAVYPTNGT
                     PNTGGGGGGRYSSSDGWGSCNPAYGGSGIVIVNLY"
     gene            7748..8074
                     /locus_tag="ACZPCG_06530"
     CDS             7748..8074
                     /locus_tag="ACZPCG_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284758.1"
                     /translation="MKREFAKAYILVDTMKEGTVQNIAMFNDYEEANRAAMAAYGDEA
                     FAEEYKWLVQAGDRYRNGIFYTVAGDVEEPAEYIPTDSEQIMRLSQENAELTMALADM
                     IGGVSA"
     gene            8071..8217
                     /locus_tag="ACZPCG_06535"
     CDS             8071..8217
                     /locus_tag="ACZPCG_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284759.1"
                     /translation="MIGNIQKSIIIRAMRIRKEAGEDPEKILKGYKNLTEEERKEILR
                     VVEQ"
     gene            8229..8519
                     /locus_tag="ACZPCG_06540"
     CDS             8229..8519
                     /locus_tag="ACZPCG_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006566107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HIR1284760.1"
                     /translation="MEYIQTILAICGGISIIGGAGAVIVKVIKPAFHLTARVQKLEEH
                     SDKDYKRLVALENMQKQQSKSLAALLNHQITGNGIDTMKQIRDELLESIIDQ"
     gene            8537..8767
                     /locus_tag="ACZPCG_06545"
     CDS             8537..8767
                     /locus_tag="ACZPCG_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HIR1284761.1"
                     /translation="MLKNCVFRADVDTIQWVKAAGIRAIKTMAQTFVATIGSAAVMGE
                     VNWPMVASASALAGILSVATSIAGLPELPAKT"
     gene            8857..9024
                     /locus_tag="ACZPCG_06550"
     CDS             8857..9024
                     /locus_tag="ACZPCG_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284762.1"
                     /translation="MANATGKRADKRTAEQRKNDAAQKRRPKGAQDTTFVTTGPATGK
                     EDERAVGTEDK"
     gene            9253..11475
                     /locus_tag="ACZPCG_06555"
     CDS             9253..11475
                     /locus_tag="ACZPCG_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284763.1"
                     /translation="MGVTKDGVTTVTIPDSMLEGAGAVRDYQVYAWVYLSDRTFGETT
                     KQIMMKVKARSKPKAFDAPGDGEIFHEAIEAVNDAAKRAEEAGDKAVSAADEAKAAAT
                     QTAEHLQATEGLAEQVETNADTVAQDKQTVTGMLSQTQQAASDAALSAQAAKLSEIAA
                     AQAQTGAEAAEDGARQYAEETGADRQAVANDKQVVSQMREAVAADRKAVEQTALQFGQ
                     TSQDALTAIGQAQGTAVGAVKAEGNKQTTAVQEAGTQAVSEVTEAKTTAVQAVTTEGD
                     TQTKRVEDAAAGIVEDREQISQNKTDIAGLVEGMTDLAPAIHSTASGSVITANDATEG
                     RPFRGLRVFGKSTQDGTPTPEAPIPIVSAGEDGSIEVKVTGKNLLTGRLYYIDYSMGA
                     GFIKNENEVSLPYAPKSETSGIGYVIPCVPEKHYAFSVTNPNENAVVAISEYKTLEDA
                     KNKENAIGFVVPSSASPYKSVYTSKGNGVLVCWIAGKWTDGKTTIHECTESELLQLEI
                     GSEATTYEPPRIPQSLTLATPNGLPGIPVTKDGNYTDADGQQWVCDEIDLERGKYVQR
                     VVIENISGGWSLKETSDRPGRFIRTGLSNKYANGGIIALCSIASWKSWGSSDRDNRYV
                     FALSMTTFYISPPSDISMTADELNAKLNALKDPVVVLGQLATPIERDLTPEEIAAYKA
                     LRTYGPTTVVSNDAGVHMEVTYVADTKNYIRNLEQRLSAKLVNIQSALISQKTSGGGI
                     "
     gene            11517..12491
                     /locus_tag="ACZPCG_06560"
     CDS             11517..12491
                     /locus_tag="ACZPCG_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284764.1"
                     /translation="MTGKTEQMKTTGKNLLKPNDYNVYHELPLKAGTVVTLMTNGQLS
                     EGGNIKFIGMENENVWFGINKGQTRVCHSIGNKDVKGFYNLLTKKEGLEYMLAIGDVK
                     IFEPYSGSFPSPSPDWEQPIEITDQPITITIKGGTEQQSITLTPPRPFTKWDRLEKID
                     GVWCWVYQSKVLSGAEIKKNFVGMHNSGSMMIKISTLGAMDEQNDAVSDKFIYSTQSV
                     STLKNGEFRIIYGNAYLKIDGITTDEEGRQWLESNDIIIIAQASAPEYIPLAASEQAQ
                     LNALTMYAGTTEITNNGGCTMDLTYTADTKTYIDNKLAAISAAMIGGT"
     gene            12491..12940
                     /locus_tag="ACZPCG_06565"
     CDS             12491..12940
                     /locus_tag="ACZPCG_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284765.1"
                     /translation="MYDIIKNVITSGSYELTDILKKIDTIWLQGALTEEQRTELIELA
                     RNSADPENSYAPLQSQIDTLYTNMTEMGKTILSLADKITKMEGGSVMPPETEEYPAWV
                     QPTGAHDAYNTGDKMTYTDGKRYICQMDNCVWGPDAYPAGWKLAEEE"
     gene            12940..13674
                     /locus_tag="ACZPCG_06570"
     CDS             12940..13674
                     /locus_tag="ACZPCG_06570"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0016019 - peptidoglycan immune receptor
                     activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045087 - innate immune response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein family
                     protein"
                     /protein_id="HIR1284766.1"
                     /translation="MEIRKEIKQINCYAGQNRPAWIVIHETDNYSKGAGALKHSEAHR
                     NGNLSTSVHWYVDDKVAVQTLDYRDGAYAVGRQYGTPLVPGVTNTNSINIEICVNPDS
                     NYDTARANCVELVRQIMAETGISADHVIRHYDAKRKHCPRKMLDQPQLWTDFKNVLNQ
                     PTYATGWHHDNNGWWYADTQHNYYRSCWQTINHHRYYFNEDGYALTDWHQIDGKWYYF
                     EPTAGHPLECALYVTDADGAQRPGEF"
BASE COUNT         4104 a         2813 c         3919 g         3274 t
ORIGIN      
        1 gcagggcgat cctgagctat gttacgcaca cttcgccctg caaagacttc atatcaggcc
       61 tggggtactg gccggtatct gcggggctaa tgatccggtg ggaccaagag aacgcgcttt
      121 tatttatgcg agcattgacc tcagggtaaa agaagaaaag aggcaggcgg ctcaactgtc
      181 ttaggaagga ggaagaaatg ccaaccttaa atgcaatgtt caggctgatg gacggataca
      241 gcagccagat caagaagatc gttgagaata ccgataaagc ggcaaaggcc atcttagggg
      301 ccagtaagaa aacagatggc ttcaacgatt cgctgaaacg cacaggggcg gctgccagtg
      361 tggcaaactc cggtttgtca aagctggtaa aaacagccat gagccttgcc gcagtaaaaa
      421 agggcatgga tttgacggat acatacacca acaccaatgc tcgactgggt atgatcactg
      481 gcagtctcga agaacagagg gcgcttcaga aggatgtatt tgcggcagca ggccgtgccc
      541 gcggtaatta cacagagatg gccaacgcta cggcaaagct taagatgctt gccggagata
      601 ctttcgggag caatcaggag gcagtaggat ttacagaact gcttacaaag tccctcaaga
      661 tatccggagc cagtacagcg gaacagaact cggcttttct ccagttgacg caggccatga
      721 cagcaggaaa gctgcagggg gatgaatttc gttccattat ggaaaatgcc cccatggtgg
      781 cggatgccat tgctcagtat atgggtaaga gtaaggcaga gctgaaggaa ctttcctctc
      841 atggtgcaat tacagcggac atcatcaaag gcgccatgtt tagtgcagca gatgagatca
      901 atgggaaatt tgcagatatg ccgcctacct ttgcagatac ctggcagaag attaaaaatg
      961 caggtatgca ggcgcttggg ggagtgtttg aaaaggcgaa tactgtttta aactcggcag
     1021 gtgtacaaac ggctctgaat aattttattg gattgattta cttggcagga gaggcaacag
     1081 aaggatttat agacttttgc attgctgcct ggcctatggt ttcaccattt atctgggcgg
     1141 cagcggcagc tctgggggct tatgcagcgg cacaaatcgt gtctaatggg ctgtcattga
     1201 tttcggctac cggatttggt gcacaagcaa taggagctgg aatttatgca ttggctttgt
     1261 gggccacaac aggggctact tgggcagaaa caacggcgat gctggggctc aactcttcat
     1321 tgtatgcctg tcctcttgtt tggattgttg gccttgtttt agttttaact gctgcttttt
     1381 atgctggagt ggctgcggtc aatcattttg cggggacttc gatcagtgct accggcttga
     1441 ttgggggagc attctttgcc ttgtttgcgt tcgtttataa ccattttgtt tatccgataa
     1501 tagcagtttt ttctatgttg gcaaacttca ttggaaatac ttgttttaac gatgcgacag
     1561 cagcggttaa agtgttattt ctggatatgg cgaatgcctg tatcggttat gtgcttaata
     1621 tggcacggtc gattgaaaat attgtcaata aaatacctgg ggttacggta gatattactt
     1681 caggactgga aggattgcag acaggacttg aaactaagat cactgcaatt aaggatgaaa
     1741 gtggctggaa agagtatgtg aaacaaccag aactgctgga ttactcagaa atggcgtcga
     1801 aggggtacaa cgcaggaaaa aatctggcag acaaagcctc taacctgttt tctggtttta
     1861 atcccagtct ggacggagca ggcggatcag gaattgattt ctcttcgttt gccactgccg
     1921 gaaaccctgc cactgtaaag ggtacaggga aaggcggagc ggtaaaggta gaaacagaaa
     1981 aagaggatat tgagtggatg cgtaagcttg cggagcggga ttatgtggcc cggatcgccc
     2041 agaataccct tgcgcccaat atccgtgtgg agttttctgg cccgatcacc aaggaagcag
     2101 atacagacgg tcttgtaggc catgtagtgg atcgcctcaa ggaagtaatt gccactgcgc
     2161 cagagggggt gcctgaataa tgtcatattc tgtctatttt aaatatgggg gtaagaaata
     2221 caagctacca gtcaatcccg aggagatcaa gcggagcagg gagttaaata tagagaatta
     2281 tcaggtactt gggacagggc aggtttctgt cccttcttac tacagtctgg aggagttcag
     2341 ctttgaagcg gaattcccca gccaggacta ccactacatg aattctggtt ccagagcgga
     2401 cgcagattat tacgaaaaga tgttccggaa ggctcagaag aacatgaagc cggtccgctt
     2461 tattgcctcc aatgacatta cagatgatat cagtgtaatg gtattggtta agagcgtgga
     2521 ggcagtagaa aaggccggtg aggaggggga taagtacctg agtattaagc tgcaggaata
     2581 caaggcccca ggcaaacggt atgttgcagt acagacggtt gcagctactg ttaagcaaga
     2641 ggatacagca gcggtagcag aaaccaatcc ggcggtgacg gagaataaga cccatacggt
     2701 ccagtctggg gacactcttt gggggatcgc aaagaagtat tacgggaacg gcagccaata
     2761 tacaaagatt gcatcagcca accccgatat taagaatcct aacctgattt atccaggaca
     2821 ggttctttcc attccggtgt aggaggtgac agcgtggagg tactggtaga aagccaggga
     2881 tatatttatg atatttcaga aatgtgcaag gaaatttcat ggacagaagc cttgaatgaa
     2941 ggagccagct gtatggatat ctcttatatc agtgacggcc tgatactcca gaatggggat
     3001 gcgatccgct tgacagacaa tgatcagacg gatggcatct tttttggcgc tgcatataaa
     3061 gtgtcaggag acagcggaga cggaggaacc ggccagatag taaccatcaa agcatatgac
     3121 cagctccgga gagcaaagaa taaggatatc attgtgctgg aaaatgggac gcttaaaaat
     3181 ctggtagaaa atatgtgcac atttcagacc atgacgccgg gaactgttga ggatcccggc
     3241 tttatcattc caaccattgc tgattatgaa aagtcctggc tggatcacat tgtgcaggct
     3301 gtttcggata ctctgattgg tacccaggag cattattgcc tgcgtgatga gtacgggaaa
     3361 gtgtgcctgt ggaatatgcg gaacctgcag cttcctttgg tcctggggga caacagtctg
     3421 tgtacgggct acagttggga gaagtctatt gatgatgatt attacaatca gatcaaggtt
     3481 gtatggaaaa atgaggatac aggtcagatc gatgtggggg ccgcccatga ccaggaggcc
     3541 gtgaaccggt atggcctttt gcagtacctg gaatcctctc cttcaggcgt ggataatgca
     3601 gccaaggcac aggagagagc caataacctt ctgaagctgt ataaccatga gaaggaaacc
     3661 ttaaagctgg agtgcctggg agatcttcgg gtacgggctg ggaatagtgt gtacggcagc
     3721 attgccgata ttgcattaaa tcggaggctg attgtaaaga aggtaacgca cgattttctg
     3781 cccgtgcaca caatgaccat ggaggtgatg gcaggtgaat gaccgtggaa gtgttcagga
     3841 gctattcaat ctgataaaaa cggtggttga taactacatg aaaagccgta agccagccgc
     3901 tgtgcttgta ggaacctata caggaaatgg cgttatgatc gagaaacttc cgatacccat
     3961 gagtatgatt acaggaaatg caaagagcca gctggtttcg ggagataaag tacggcttct
     4021 tcgtaatgat cgaggccatg aatattacat tctggagatt atcggaaagc catatcagac
     4081 agcaggaggt acataatggc agaacttaca acggatctga ttgttcagga acagacctat
     4141 aaggcccgta cctacggatt atcggaaact aagatagagg gtttcgtgga tgaactggcg
     4201 gcgttaaaac aggccattta taaaatcctg tctacggagc agtatgaata tcctatttat
     4261 agttttaaat atggcatagc ctggaaagag ctgatcgggg aggaacgggc ttatgtgaga
     4321 gcggagatgc gtcgcatgat cgaggaagcc cttttgcagg atgaccggat ccgggaggtg
     4381 gacgggttcc ggtttgagtt ttctggagac agctgccatt gttcctttca agtatccagc
     4441 atttacggtg aaatacagat agagaaagag gtggctgtat gaaaatgacc tatgaggaac
     4501 tgctgcaggc aatgcttgac agggtgccaa gtgatgtaga taaacgggaa ggcagtgtta
     4561 tttatgatgc attagctccc tgtgcttatt ttctggccca gcaggatttt cagctggaca
     4621 acttcataga tctggtcttt ccagatacag cccttggtga gtatctggac cgggcggcag
     4681 aggcctacaa tgtttccaga aagccagcca caaaagcaat gcgtatgatg actacttccg
     4741 gacgggtgga gattggcact cgttggggaa tcaataatct tgtctatcgt gtgaaggaag
     4801 aagaatcaga aacagaatac agggtggagt gtgaaacacc tggtgtgatc ggtaatcagt
     4861 acagtggagc aatgcagcct atttccaata taacggggat tactgcagag ctgggggaca
     4921 ttattactcc aggcgcagat gaagagacag atgaagcctt gcgtgagcgc ctgtatacca
     4981 aaatcagact tccggccact tcaggaaatg tttaccatta ccaacagtgg gctttggagg
     5041 tttctggaac cggcgcagca aaagttttct cattggcaga cggtcctggt acggttactg
     5101 tgcttgtggt agacagcgat aaaaagatat catcttccct gccggagacc gtggcagcat
     5161 acattgaaac gatgcgcccg atcggtgctt cggtaacagt taagagtcct gaggctgtta
     5221 cgatcaatgt gaaagcaaag atcatgaggg acggaagcaa gactctggag gatattcagg
     5281 cagcgtataa attggcagtg gattcatttc tgagggaaac cgtatttgct acatatagga
     5341 tcagtcatgc aaagcttgga agcctgctgc tggatattcc gggagtagag gattttgagg
     5401 gatttctttt aaatggcggg acaggaaacg tgacagtcgg ggaaaaacag attcctgtga
     5461 ctggaaccat tgagctgacg gaggtgagcc ggattggaac tgattaaact gcttccggat
     5521 tattatgaaa agaatgtgac catgcagaca ttacagggac tgctttccga ggtaacggat
     5581 gatctggagc aggggctgag cagtacaata tcagaatgct ttgcatctac agcgtcagag
     5641 ctgttggcaa ggtatgagca gcttttaggg ctggaggtgg acgtatcaaa accggatgat
     5701 tttcgtcagg agcggatccg cgccaagata tccggtgttg gaactaccac aaaagagatg
     5761 gtaaagaatg tagcaagtag ttactccaat ggcgaagtgg aagtaattga agatgcggcg
     5821 cactatcgtt ttgtgatccg ctttgtaggt atactgggga ttccgggaaa tatggcagat
     5881 ctgaagctta ctattgagga aattaagcct gcacatctgg tggtggagta cgaatacatc
     5941 tataacgtct ggagcgatgt ggagatgttg acctgggagc aggccgcagc acgtacttgg
     6001 gaagagataa ggagtaatag gataaatgga attaacagac aaatataaat taaaaaaacc
     6061 aggggccagc gattatataa atgtagaaga ttttaatgag aatgcagaaa tagtggatcg
     6121 ggagatggag aagcatgaaa atttgcttga ccgcctgtat gccgccccta ttcgtattac
     6181 ccttaccgct tctggatggg ctggatcgtc agtaccctat acacagacgg tagcagttcc
     6241 gagcattaag acagaggata atcctgtttt ggtcagtcag ttggcggacg gtgcaacgat
     6301 agaagttcag aaggcataca gtaaggcatt tggtattgtg gcttccggta caggacagac
     6361 cggagatgga acagtgacat tcaaagtcta caaaaagcca gctatagata tcgtagttgg
     6421 gcttaaatta taaggaggag ctgaacatat gggaaatata ctgatgacag gttctggcgg
     6481 aggcggagca gggagtgatg actgcaccgc aacggccgca gaactgctga aaggctacac
     6541 gggcatattg aaaggatcgg atgatgaacc ggttcaggga atattggaat tgacagggaa
     6601 cgcccaggca gcccatgtgc tgaatggaga gacgttttat agcaatgatg ccaaaaccaa
     6661 acatacaggt aatatgaccg ttaatagttt actgtctttt agtgttgccg cctattccgg
     6721 aagaagggtc atagctacct ggacaaatcc gaatcaggcg gctggccgac catatagcgg
     6781 agttatcatt cgatatgaca cgggagggta tcctggcgca tctggcggag ttcagattta
     6841 taaaggagcg gggaacaacc attctgccgg agctgcttca caggcgtttc ttgatttgcc
     6901 gaatctgaat acaagatatt tcttatcatg tactccgtat gttactacca gtattggcga
     6961 actgtttggc ccaactctta atgcagagat tacaacagga ggctctacca gcagaacgtt
     7021 tacagcttcc ggttcttttg ttgtagaagg atttactaag atggatgtat ttcttgttgg
     7081 aggaggcgga ggggctgggt atgctggtgg aggaagcgga tatactgcta ctcaatatgg
     7141 gattccaatc actacgggaa ataatgtttc ggttgttgtt ggaggaggtg gcggaccagc
     7201 atctaatggc ggtacctcat ctattagcgt aaatggtgcg gtgaaagctt cagcagctgg
     7261 aggaaatggt gctcctgatt cgtatggagt agctgccgga aatgggggat ccggaggcgg
     7321 aggaagctct acaggtagag taagaggagg aaatggtggt tctgatggcg gaaatggagc
     7381 atttcctaaa ttcccaaatg gtacaggaca gggaagaacc acgagagcat ggggtaattc
     7441 tgtgggtgct ttatatgcag gcggcggagc tggcggcaat aaccactata acaatagaaa
     7501 taatgagggg gaccgcgtag aatattttaa tttaggtgga actggaggtg ccggaggcgg
     7561 aggaaatggc ggtggatggg aaggaaccaa ttacggaaaa gctgtctatc caactaatgg
     7621 tactccaaat actggtggtg gcggaggtgg cagatactca tcatctgatg gctggggatc
     7681 atgcaatcct gcctatggtg gttctggcat tgtaattgta aatttgtatt aggaaagcga
     7741 gggcaaaatg aaacgagagt ttgcgaaagc atatattctg gttgatacga tgaaggaagg
     7801 cacagtacag aatattgcga tgtttaatga ttatgaagaa gcaaacagag ctgccatggc
     7861 tgcgtatggt gatgaggcat ttgcagaaga gtataaatgg cttgttcagg caggggacag
     7921 gtatcgtaat ggcatttttt atacggttgc aggagatgtg gaagagccgg ctgaatatat
     7981 tccaactgat tcagagcaga tcatgcggtt gagtcaggaa aatgcagagt taacaatggc
     8041 attggcagac atgataggag gtgtatcagc atgattggta atatccagaa gtctatcatt
     8101 atccgggcaa tgcgcatacg gaaggaagct ggagaagatc cagaaaagat cctgaaagga
     8161 tacaaaaacc tgacggaaga ggaacggaag gagattttga gggtagtaga gcagtaagga
     8221 gatatgccat ggagtacata cagacaatcc tcgccatttg cggagggata agtatcatag
     8281 gtggagccgg tgcggttatc gttaaggtaa tcaagccggc ttttcatctg acagcacgag
     8341 tccagaaact ggaagagcat tcggacaagg attacaagcg tctggttgct ctggaaaata
     8401 tgcagaaaca gcagtccaag agtcttgcag ctctgctcaa tcatcagatc accggtaatg
     8461 ggatagatac gatgaagcag atccgggacg agctgttgga atctattata gaccagtaga
     8521 agggaggtga ggaactatgc ttaagaactg tgtatttagg gctgatgtgg ataccatcca
     8581 gtgggtcaag gccgcaggta tccgtgccat taagaccatg gcgcagacat ttgtcgccac
     8641 catcggctcg gcagcggtca tgggtgaggt caactggcct atggtagcca gtgcatccgc
     8701 gctggcaggt atcctgtcag tggcaacatc cattgcaggc cttccggaac tgccagccaa
     8761 gacctgagag gaggtgatcc ataggtctcc cgtccggcag ggtcagagcc ggaaagaaac
     8821 tactattaca tcattttgaa agtgaggaaa aagattatgg caaacgcaac aggaaagaga
     8881 gcagacaaga gaaccgcaga acagagaaag aatgacgcag cccagaagag aagacccaag
     8941 ggcgcacagg atactacttt tgtaaccacc ggccctgcaa ccggcaagga ggacgagaga
     9001 gcggtaggca cggaagataa gtaagctgtg cggcgtcgca acacagacag gccccgggat
     9061 ttcctggggc cgtttttggt tggagggaat atgattacag cagtatttac agataacgat
     9121 gattacgccc atgcctacgg cctatggcag tgggattatg gtcagcagct caggatagag
     9181 ggactgcatc ttccgacggc ggtagaaatc cactttgcgc tacaggagac cggtggtgag
     9241 gccataaccc gtgtgggagt gaccaaagac ggcgtaacaa ccgtcacgat tccagacagt
     9301 atgttagagg gcgcgggtgc cgtaagggat tatcaagtct atgcatgggt gtacctgtca
     9361 gacaggacat tcggcgagac cactaaacag attatgatga aagtcaaggc aagatctaag
     9421 ccaaaagcct ttgatgcacc cggagatggt gagatattcc atgaagccat tgaggccgtc
     9481 aatgacgctg ccaaacgggc agaagaggcc ggtgacaagg ctgtatccgc tgcggatgag
     9541 gccaaggcag cagccaccca gacagcggag catctgcaag ctacagaggg tcttgcagaa
     9601 caggtagaga ccaatgccga caccgtggca caggataagc agactgtaac agggatgctc
     9661 tctcagaccc agcaggcggc ctcagatgcg gcattatcgg cacaggcggc taagttatca
     9721 gagatagccg cagcacaggc acagacgggc gctgaggcgg ctgaggatgg agcaagacag
     9781 tacgctgagg agacaggggc agaccggcag gcagttgcca atgataagca ggtagttagc
     9841 cagatgaggg aagccgtggc ggcagaccgg aaggcggtag agcagaccgc tttgcagttt
     9901 ggacagacct ctcaggatgc gcttacagcc atagggcagg ctcagggcac ggctgtggga
     9961 gcagttaagg ccgagggcaa taaacagacc acagcggtac aggaggcagg tacacaggcg
    10021 gtcagtgagg ttactgaggc caagactaca gcagtgcagg cagtcaccac ggagggcgat
    10081 acgcagacca aaagggttga ggatgcggct gccgggattg tggaggatag ggagcagatc
    10141 agccagaata agactgatat agccggtctg gtggaaggga tgacagattt ggcaccggca
    10201 atccatagca cggcttctgg ctccgtcatt accgccaatg atgccacaga aggccgcccg
    10261 ttccgggggc tgagggtgtt tgggaagagt acgcaggatg ggacaccgac accagaagca
    10321 ccgataccga tagtgagtgc tggggaggac gggagtattg aagttaaggt gacagggaag
    10381 aacctgttaa caggtaggct gtactatatt gattactcaa tgggggctgg ttttataaaa
    10441 aatgaaaatg aagtatcatt accatatgca ccaaaatcgg aaacatctgg tattggatat
    10501 gtaatcccgt gtgtaccaga aaaacactac gcatttagtg ttactaatcc taatgagaat
    10561 gccgttgttg ccatatcaga gtacaaaacg cttgaggatg caaaaaataa agaaaatgcc
    10621 attggttttg ttgttccaag ttcagcttct ccttataagt cagtctatac atcaaaggga
    10681 aatggagtac ttgtgtgttg gatagcagga aaatggacgg acggaaaaac aactatccat
    10741 gaatgcacag aatccgagtt attgcaactt gaaatcggtt ctgaagcaac cacctacgag
    10801 ccaccacgca ttccccaatc cctcaccctc gccactccta acggtttacc cggaattcca
    10861 gtgacgaaag atggcaacta cacggacgca gacggtcagc agtgggtgtg tgacgagatc
    10921 gacttggagc gtgggaagta tgtgcagagg gtagtgatcg aaaacataag cggtggctgg
    10981 agtcttaagg agacgtcaga ccgccctggg cgattcatcc gaaccggact atccaataaa
    11041 tatgcaaatg ggggaatcat cgcattatgc agcattgcaa gctggaaaag ttggggatca
    11101 agtgacagag ataatcgata tgtatttgcg ctttccatga ctacattcta tatttctccg
    11161 ccctcggata tatccatgac agcggatgaa cttaatgcaa agcttaatgc attaaaagat
    11221 cctgtggttg tactggggca gcttgccacc cctatcgagc gggatctcac ccccgaagag
    11281 attgctgctt acaaggctct gcggacatat ggcccgacta ccgttgtgag caatgacgca
    11341 ggggtacata tggaagtaac gtatgtagca gatacgaaaa actacatacg caatctggaa
    11401 cagcgtttaa gtgccaagtt ggttaatatc cagtcggcat tgattagtca aaaaaccagt
    11461 ggggggggga tttaacaata gctgatagct caccactgcc ggtagaggag ttttccatga
    11521 ccggtaagac agaacagatg aagacgacag ggaagaatct gttaaaacct aacgattata
    11581 atgtatatca tgagttacca ttgaaagctg gcactgttgt tacattaatg acaaatgggc
    11641 aattgtcaga gggcggtaat attaaattca tcggaatgga aaatgaaaac gtttggtttg
    11701 gtattaataa aggacaaacc agagtttgtc attctatagg gaataaggat gttaaagggt
    11761 tttataacct acttactaaa aaggaagggt tagagtacat gcttgccatt ggtgatgtaa
    11821 agatttttga accctattcc ggcagcttcc cttccccctc acctgactgg gaacagccca
    11881 tcgagataac tgaccagccc atcaccatta ccatcaaagg cggcacagag cagcagtcca
    11941 tcactttaac accgcccaga ccatttacca agtgggatag attggaaaag atcgacggtg
    12001 tgtggtgctg ggtgtatcag agcaaggtct tgtcaggagc ggagataaag aaaaacttcg
    12061 tggggatgca taattcaggt tctatgatga taaaaatttc aacacttggg gctatggatg
    12121 aacaaaacga tgccgtaagc gataaattta tttattcaac tcaaagtgtg tccacgctta
    12181 aaaacggaga atttcgaatc atatacggaa acgcttatct caaaatagat gggattacaa
    12241 cggatgagga aggaaggcag tggcttgaat ccaatgacat tataatcatt gcacaggctt
    12301 ccgcacccga gtacatcccc cttgctgcat ccgaacaggc ccagctcaac gccctcacca
    12361 tgtatgcggg cactaccgaa atcaccaaca acggcggctg taccatggat ttaacctaca
    12421 ccgcagatac caaaacctat atagataaca agttagcggc tatcagtgcc gctatgatag
    12481 gaggtacata atgtacgata tcattaaaaa cgtaatcacg tccggaagct atgagctgac
    12541 ggacatcctt aaaaagattg ataccatctg gctccagggc gctctcaccg aggagcaacg
    12601 cacggagctg atcgagctgg cccgtaatag tgctgaccca gaaaacagct atgccccgct
    12661 ccaatcacag attgacaccc tctataccaa catgaccgag atgggtaaga ccatcctcag
    12721 cctggctgac aaaattacta agatggaggg cggtagcgtg atgccaccag agacagagga
    12781 gtatcctgca tgggtacagc ctacaggtgc acatgatgcc tataacacag gcgataagat
    12841 gacatacacc gacggcaagc ggtatatctg ccagatggat aactgcgtat gggggccgga
    12901 tgcgtatccg gctggctgga aattagcgga ggaagagtaa tggagatacg aaaagagatt
    12961 aagcagataa attgttacgc aggtcaaaac cgtcctgcat ggattgtaat ccatgagacg
    13021 gataattaca gtaagggtgc aggtgccctt aaacactccg aagcccatcg aaacggcaac
    13081 ctgtccactt cagtccactg gtatgtagat gataaagtag cagtgcagac cttagattac
    13141 agagacgggg cctatgcagt aggtaggcag tacggtacgc ctctggtgcc gggagtaacc
    13201 aacaccaata gcattaacat tgagatctgc gtcaacccgg actctaatta tgataccgcc
    13261 cgtgctaact gcgtggaact tgtacgccag atcatggcag agacaggtat cagtgccgat
    13321 catgtaatcc ggcactatga tgcaaagcgc aagcactgcc cccgtaagat gctggatcag
    13381 ccgcagttat ggacagactt caaaaatgtt ctaaatcagc ccacatatgc aacagggtgg
    13441 caccacgata ataatggctg gtggtacgca gatacacagc acaattatta cagatcatgt
    13501 tggcagacga ttaaccacca taggtattat tttaatgaag atggttacgc tttgacagat
    13561 tggcatcaga ttgatggcaa gtggtattat tttgaaccta cggcaggcca tcctcttgaa
    13621 tgcgcgttgt atgtaacaga cgcagatgga gcgcagagac caggggagtt ctaaatcaaa
    13681 aaaaggagaa gcgactgcta tctaatagac aatcgcttct cctttaactt tttacattcc
    13741 catatggggc aaagtcaaaa attaaaacac ttaaatccac gcctcaagct agctgtggcg
    13801 accatatata tgttaatagt atatcatatt atttttttga ttttcaagag atttctaaat
    13861 ttcccgtgtt gcattttcgt gttgcatagt agttaaaata ctgctacaaa ttgtattatt
    13921 aaccacaaaa attgaatata ttataataag gaaacaacgc atttataagg aatcttggat
    13981 ttaagcggtt tttggatgca taattttagg ggttcgaatc ccgtctcgcg ctttttttat
    14041 ggctggaatt gcttgatttt tcgagggttc cggccttttg cgttctctaa aaatcggtag
    14101 acaatataag
//
LOCUS       DBMDUH010000058        14083 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_2336_length_14083_cov_7.996352, whole genome shotgun sequence.
ACCESSION   DBMDUH010000058 DBMDUH010000000
VERSION     DBMDUH010000058.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 14083)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14083)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14083
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2336_length_14083_cov_7.996352"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..104
                     /locus_tag="ACZPCG_06575"
     CDS             <1..104
                     /locus_tag="ACZPCG_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR1284767.1"
                     /translation="SDLLGHESIETTRIYLRRTSTEQARIFNQVVNW"
     gene            120..995
                     /locus_tag="ACZPCG_06580"
     CDS             120..995
                     /locus_tag="ACZPCG_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003501358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR1284768.1"
                     /translation="MGQRKKPKEPEEEFILYLKEHGASEHTVDSCLAALRLFFRLYGE
                     ISAENLDAFRRYLMEHYKISTVNSRIYGINRFLAYCSECKNSYETSGKWTLPEKNFLL
                     GVIRSQNPPFLDNIISEKDYEKLIDNLKKDRDMKWYFAVRFLACTGARVSELIQIKRE
                     HIQLGYMDLYTKGGKVRRIYFPDYLCSEASGWMDSQNMGSGFLFRNRKGGAVSPRWIN
                     SQLKLRARQYDIPEETVYPHSFRHRFAKNFLARCNDIALLADLMGHESIETTRIYLTK
                     SSQEQREIIDSIVTW"
     gene            1137..1901
                     /locus_tag="ACZPCG_06585"
     CDS             1137..1901
                     /locus_tag="ACZPCG_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR1284769.1"
                     /translation="MSDNTICVYRYAVEQFYRLYPQLTSRNLQLYKVYLLEHYKPQTV
                     NLRLRAINCFIEDKKLRSSPAPMIRIQQKTWLDKIISQADYEYLKQRLLEDEEMVYYF
                     LVRLMAATGVRVSELVTFQVGDVHCGFKDLYSKGDKMRRIYIPSNLRKAFFKWDDFTA
                     RTDGALFLNRFGAPITPSGIRSRLKTFAARYGLDPEVMYPHSFRHRFAKNFIEACGDI
                     SLLADLLGHESIETTRIYLRRSSAEQYRIVNKVVDW"
     gene            1917..2770
                     /locus_tag="ACZPCG_06590"
                     /pseudo
     CDS             1917..2770
                     /locus_tag="ACZPCG_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007719818.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            2802..3395
                     /locus_tag="ACZPCG_06595"
                     /pseudo
     CDS             2802..3395
                     /locus_tag="ACZPCG_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007719878.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
     gene            complement(3734..5470)
                     /locus_tag="ACZPCG_06600"
     CDS             complement(3734..5470)
                     /locus_tag="ACZPCG_06600"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003525019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HIR1284770.1"
                     /translation="MKDYMKIYQEWLSNPYFDEATKAELKAIEGDENEIKERFYMDLE
                     FGTAGLRGIIGAGINRMNIYVVRRATQGLANYIIKQGAADKGVAIAYDSRNMSPEFAM
                     EAAMTLAANGIKAYKFESLRPTPELSFAVRELGCVAGINITASHNPPEYNGYKVYWED
                     GAQFTPPHDKGVTEEVLAIEDLSSVKTTDEASAIAAGKYEVIGKEIDDKYIAQVKAQV
                     VNQKAIDEMQDQIRIVYTPLHGTGNIPARRVMKEIGFTHVYVVPEQELPDGNFPTVSY
                     PNPEAKEAFELGLKLAKEKDADLVLATDPDADRLGVYVKDAKSGEYIPLTGNMSGSLL
                     CEYVLSQKQAEGKIPADGQVVKSIVSTNLIDAVAKAYGAELIEVLTGFKWIGKQILKN
                     ETTGTGTYLFGMEESYGCLIGTYARDKDAISATAALCEAAAYYKQKGMTLWDAMVAMY
                     EKYGYYKDSVKSIGLSGIEGLAKIQSIMETLRNNTPAEVGGYKVVSARDYKLDTIRDM
                     ETGAVKPTGLPASNVLYYDLEDGAWICVRPSGTEPKIKFYFGVKGTSLEDADQKEADL
                     GAAVMAMVDKMM"
     gene            complement(5580..7274)
                     /gene="rho"
                     /locus_tag="ACZPCG_06605"
     CDS             complement(5580..7274)
                     /gene="rho"
                     /locus_tag="ACZPCG_06605"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HIR1284771.1"
                     /translation="MREKLQTLPLAELKELAKSQGLKGISTLRKAEIIELLCRENEAA
                     AQSEAVLQPDSGSSEGREAQSSREPQSSKENGEPRDSEKSRDSGKPRDGRPQRDSSRQ
                     TRFVRQPRTSYGQQNSREASRDSAESREPKENRESRDNRESRESRESRETGDIRDTRE
                     TRADVRESRPEPRSDASGIAGQDMAELDSGIEANGILEVMPDGFGFIRCENFLPGEND
                     VYVAPSQIRRFNLKTGDIIVGNRRIKAVTEKFAALLYIKTVNGYPLSATERRPNFEDL
                     TPIFPNERLHMETRGERNSVAMRVLDLLSPIGKGQRGMIVSPPKAGKTTLLKQVARAI
                     TVNHPDMHLMILLIDERPEEVTDIREAIVGPNVEVIYSTFDEQPDRHKRVSEMVIERA
                     KRLVEHGRDVIILLDSITRLARAYNLTVAPSGRTLSGGLDPAALYMPKRFFGAARNMR
                     EGGSLTILATALVDTGSRMDDVVYEEFKGTGNMELVLDRRLSEKRIFPAIDILKSGTR
                     RDDLLLNRQEAEAVDIVRKATNSLKPDEAVERILDLFSKTRNNYEFCEMVKKIRLL"
     gene            complement(7484..8800)
                     /locus_tag="ACZPCG_06610"
     CDS             complement(7484..8800)
                     /locus_tag="ACZPCG_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HIR1284772.1"
                     /translation="MNLFDYMRENTMEKEAPLASRLRPRTLDEVVGQQHIIGKDKLLY
                     RAIKADKLGSLIFYGPPGTGKTTLAKVIANTTSAEFKQINATVAGKKDMEEIVAAAKD
                     SRGMYGKRTILFVDEIHRFNKGQQDYLLPFVEDGTLILIGATTENPYFEVNSALISRS
                     RIFELKPLETQDILELIQRAITDVERGMGTYDAVIEPDAARFLADAANGDARAALNAV
                     ELGVLTTDRSPDGKIHIDLAAAQECIQKRAVRYDKDGDSHYDTISAFIKSMRGSDPDA
                     AVYYLARMIYAGEDIKFISRRIMICAAEDVGNADPQALTVAVSAAQAAERIGLPEAQI
                     ILSQAVTYVACAPKSNAACCAIQDAMEAVKTERAMPVPVHLQDRHYKGAGALGHGDGY
                     LYAHDYPKHYVRQQYLPDGMEGRIFYRPSDNGYETQMKAHMEWLKN"
     gene            complement(8814..9557)
                     /locus_tag="ACZPCG_06615"
     CDS             complement(8814..9557)
                     /locus_tag="ACZPCG_06615"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006773105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR1284773.1"
                     /translation="MELHIINQSTNLPGGLNVKWKHRVHLLSAVLSADFILLAYLICL
                     LAAPVTVREASAPEGTFEPEKYLALTFDDGPHPVHTPVLLDGLKERGVPATFFLMGSC
                     IPGNEALVKRMQEEGHLIGNHSYNHVRLTKAGPAAVCEAVDRTSGMIEDITGSRPQYM
                     RPPYGDWNEELECQSGMTTVLWSVDSLDWKLRNQKRIVKKVLKEAEDGDIILMHDIFD
                     TSVSAALELIDILQREGYTFVTADELMID"
     gene            complement(9642..11504)
                     /locus_tag="ACZPCG_06620"
     CDS             complement(9642..11504)
                     /locus_tag="ACZPCG_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HIR1284774.1"
                     /translation="MKKRQEMRKRCQALVLAGILGAGTLGSAYTAYAAGPGEMPASVG
                     ASAAGGPGETGGSGGTGNVPSASSTSENAKGADQTNPNAWKKVNGVYQMPDGSAIQNV
                     LARGIDVSRWQGEINWSQVAADDVSFVMLGTRSKGAVDPYFHRNIQQASAAGVKVGVY
                     IYSLATTVEMAEAEADFVLDLIHDYPVSYPVAFDMEDSTQGNLSKEELAAIANAFCKK
                     ISDSGYYPIVYANENWLKNKLDMSLMDYPVWVARYSARPSYQNPVMWQATSTGSVKGI
                     NGNVDIDFQFKDFSGQIPANTWRTINGKRYYYSNYAKQKNAWAQDSSDWYYMDSEGLA
                     STGWITVSGSRYYLDETTGKMQTGWRQDQGKWYYLGSSGAVKKGWIEDNGAWYYTDSN
                     GIMQTGWLDADGKRYYMESSGKMAVGWTNQNGKWYYLDASGALAKGWIQDNGSWYCSD
                     TNGVMQTGWLSEGGKRYYLKGSGVMATGWREMDGAWYYFDGSGAMTTGMIEVNGLHYY
                     MDPSTGRMAAGQTLEIGGAVYEAAADGHLIPQGQEESGSTNQESASDSAGTASGSSGT
                     SSNPIIIPSNGSGNSSSSQNVPEPSAPVTGINGGPGVSSAPSQGQVAAGEKPGV"
     gene            complement(11624..12667)
                     /locus_tag="ACZPCG_06625"
     CDS             complement(11624..12667)
                     /locus_tag="ACZPCG_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002593969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HIR1284775.1"
                     /translation="MKRKKCLAALTAAGVISAMALGMAVTSYAADGWTKTNDHWTYIN
                     QGKTHTGWLQTSEGYYYMDLSTGYMTTGFKQIDGKWYFFRPGGLMAKGWINPEYGKWY
                     YMLEDGTMVTGWLRIGDDYYFMRGDGSMGTGWREMDGAWYYFQENGKCVVNAWAQIQD
                     NWYLFGTDGKMMTGWAKVDQDYFYLNTDGRMLIGWLSDSEGSKYYMDMTNGKMSVGWK
                     QIDGKWYYFNSNGHLMTGWILIDSKYYYLNPADGGVMTAGTTLNINGTSYNFDASGVC
                     QNANGVSAQTPAAGNQSSGGGVTGENGGPGVSKTSGSSSSSGPGSSSSGTTSSSGPSS
                     SGSLEAGRTDGPG"
     gene            complement(12800..>14083)
                     /locus_tag="ACZPCG_06630"
     CDS             complement(12800..>14083)
                     /locus_tag="ACZPCG_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002593968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR1284776.1"
                     /translation="SFQKGDTKEWCFTFTKKGRITDVNVGGRDQWVMYGPVYLSREFS
                     ARFLPVLEAYYETPGTEQFYWEQPFVDMLKGEAKRRLEAEGGTLLARAEEASGWKASQ
                     WDQIEMDVNRQPENQVYEFENLEELRLFDERYQNHSDNAAMDLVSRVFKVPESHITDI
                     RCLKAGMTNKSFLFRVDGAHCICRIPGPGTELLINRREEKEVYDTVASLGITEEVLYM
                     NPDTGYKIARYYENARNAAADNWKDMEICMGIVRKLHQSGLTVDHSFDIRERIDFYER
                     LCLQHGGIPFEDYEKVRGWMNELMDRLDQMDRPQCLCHIDANVDNFLMFEDGSAKLLD
                     WEYAGMCDPIMDISMSAIYSYYDAEQTEKLLEIYLERKPSEEEYYSVFANAALGGFLW
                     CLWAVYKAALGEEFGEYTIIMYRYAKGYYKKLKAL"
BASE COUNT         3325 a         3735 c         3231 g         3792 t
ORIGIN      
        1 tatccgatct tcttggacat gaaagcattg aaactacccg aatttatctg cggcggacca
       61 gcactgagca ggcgcggatt ttcaatcagg tcgtaaactg gtaggaggcg ggatgtatca
      121 tgggacaacg aaaaaagccg aaggaaccgg aggaggaatt tatcctgtat ctgaaggagc
      181 atggggcatc agaacacacc gttgacagct gtctggctgc tctccggctc tttttcaggc
      241 tctatggaga aatcagcgca gagaatctgg atgcattcag acggtatctg atggagcact
      301 ataaaatttc taccgtaaac agccggatct atggtataaa ccgctttctg gcttactgtt
      361 ctgagtgtaa aaactcttat gaaacaagtg ggaaatggac acttccggaa aaaaatttcc
      421 tccttggcgt aatccgcagc cagaatcctc catttctgga taatattatt tcagaaaagg
      481 attatgaaaa gcttatagac aatctgaaaa aagaccggga tatgaaatgg tattttgcag
      541 tgcggttcct ggcctgtact ggagccagag tcagtgagct gatccagatc aaacgggagc
      601 atatacagct tggatatatg gatctgtata caaaaggcgg aaaggtgcgc aggatttatt
      661 tccccgacta tctctgttca gaagcttccg ggtggatgga ctcgcagaac atgggatccg
      721 gttttctgtt ccgcaaccga aaaggcggag ccgtttctcc ccgctggatc aacagccagc
      781 ttaagctccg tgccagacag tatgatattc cggaggaaac agtatatcca cactccttcc
      841 gccaccggtt cgctaaaaat ttcctggccc gctgcaatga tattgctctg cttgcggatc
      901 tgatgggaca tgaaagtatt gaaaccacgc ggatctatct gacaaaatct tcccaggaac
      961 agcgtgagat cattgattct atcgtaacct ggtagctatc cagcccccac acttacattt
     1021 tctaaaaaat cggtctagga tggatgaaaa aaggaggatt cattatgaat gcgattacaa
     1081 aatcatcatc tgtccgggga ttggaggttt tcctggagca ccttcaggag aaaaatatgt
     1141 ccgataacac catctgcgtt taccgctatg ctgtggagca gttttacagg ctctatcccc
     1201 agcttacttc cagaaatctg cagctttaca aggtttatct gcttgagcat tataagcccc
     1261 agactgtaaa cttaagactc agggcaataa actgttttat agaagataaa aagctccggt
     1321 cttctcctgc ccccatgata cggatccagc agaaaacctg gctggataag attatcagtc
     1381 aggccgatta tgaatatctg aaacagaggc ttttagagga tgaggaaatg gtctattatt
     1441 tcctcgttcg tcttatggca gctaccggag tcagggtcag cgagcttgta acctttcagg
     1501 taggggatgt acactgcggt tttaaggatc tgtattccaa aggggataag atgaggagga
     1561 tttatattcc ttcaaatctt cgaaaagcct tttttaaatg ggatgacttt acagcccgta
     1621 cagatggagc cctgttttta aaccgctttg gagctcctat tactccctcg ggcatacgct
     1681 cgcggttgaa aacctttgcc gcacggtatg gattggatcc ggaggtaatg tacccccact
     1741 ctttccgcca ccggttcgcc aaaaacttta ttgaagcctg cggggatatt tctctactgg
     1801 cagatctttt gggacatgag agcattgaaa ctacgcggat ttatctgcgc agaagcagcg
     1861 cggagcaata caggatcgta aataaggtag tagactggtg aaaggagaca tatgggatga
     1921 atgtgaacaa tggaaactcc ctgctcccgg aggatcggat cgaagaattt aaaagcatgc
     1981 tttcagaaaa tggagcgtcg gaaaatacgg taagggtata tgcggcgtct gtgcgggaat
     2041 tttataaaaa tcatcaggag ctgaccgtag acgaattaca ggactataaa agagagcttt
     2101 tgaaaaaata ccggcctgca acagtaaata cacggatcta tgggatcaac caatatgtga
     2161 agtaccaaaa aaacagtgga attccggaag atttccggct tcctcctgtg cggtgccagc
     2221 agaaaccatt tctcaacaat ataatctcaa aacgggatta tgaaaaactg aaaaagggac
     2281 ttttgaagga tcaggatcta ttctggtact ttgtagtgcg gttcctgggc gctacaggag
     2341 ccagagtaag cgaattggtt cagatcaagg cagaacacat tgccgtaggc tgtatggatc
     2401 tgtacacgaa agggggaaaa tgcgccgtat ttattttccc agacagcttt gcagggaaat
     2461 ggctgtgtgg ctaaaacaga gagaactgtc atccggatat atatttacca gccgctgcgg
     2521 aaagcccatt actcccagag ggatcggctt acagcttaaa gtgcttgcaa aacgataccg
     2581 gatccggcct gaaacggtat atccccattc cttccgccat cggtatgcaa aaaattttct
     2641 tgaaaaattc aatgatattt ccctgctggc agaccttatg ggacatgaca gcatcgaaac
     2701 cacgcgcatc tatctgaccc gttcctcaca ggaacagcaa aaggtgattg accgggtagt
     2761 aacctggtaa tataatacaa ccattaatgg aggataatta tatgtatcaa tacacctggt
     2821 atcaatggct gtttattttc tatctatact gttttttagg gtggatcttt gaatcatcct
     2881 atgtttctct aaaagaaagg cgtcctgtaa accggggatt tctgcggctg cctctgctcc
     2941 ctctgtatgg tacgggagct gtgatgatgc tgtgggtatc cctccctctg agagaacatc
     3001 tccttttggt ctttctggcc ggcatgaccg tttctactgc tcttgaatat ataaccggct
     3061 ggtgcatgga acagctgttc cgggtacgct actgggacta cagcagccag gcttttaatt
     3121 tacacggata tatctgcctt tcttcttcta ttgcctgggg ggttcttacc ctgcttctga
     3181 cagaggtaat ccataagccg gtggaattct ggatcgtaaa cacctcacaa gctctgcttt
     3241 tgctctgggt tctgtttata tccgtatttt tcatccttga taccgtacaa tcagtacagg
     3301 ctgccctcaa tctggcaaag gtactggatt ccatgaccca tatgctggca gaactggaag
     3361 atatacaggt gcagttagcc ctactcaaag cagaaacaaa ccagaaactt gccaatatcc
     3421 aggaagaggc ttcccataag ctggctgccg caaaagccga ggctgcactt tacaggactt
     3481 ccaaagaaga gcagatccgc tctcttacca ggaggatgtc tgagctggcg catcagagac
     3541 agcatatggc cctccacata gatttatacc agagagggat cctgaagcgt aatccgggag
     3601 cccgttccac acggttcgaa accgcattta aagaactgcg ggagattgca gaaaaacagc
     3661 ttcacaaata aacatcgatc agccgccgga cagtacacac tgcccggcgg ctgatcttta
     3721 tactgctttt atatcacatc atcttatcta ccatagccat aacagctgct ccaaggtctg
     3781 cttccttctg gtctgcatcc tctaaggagg ttccctttac gccaaagtag aacttgatct
     3841 tcggctctgt accggaaggt cttacgcaga tccatgcgcc gtcctccagg tcatagtaca
     3901 gtacattgga ggccggaagt ccggttggct ttaccgctcc tgtctccatg tccctgatcg
     3961 tatccagctt gtagtctctt gctgatacta ccttgtaacc tcctacctct gcaggggtat
     4021 tatttctcag agtttccatg atggactgga tctttgccag accttcgatg ccggataaac
     4081 cgatagactt gacagaatcc ttgtaatagc cgtatttctc atacatggct accatggcat
     4141 cccacagggt cattcccttc tgcttatagt aggcagctgc ctcgcagaga gcggccgtag
     4201 cggaaatagc gtccttatca cgggcatagg tgccgatcag gcagccatag ctctcttcca
     4261 tgccaaagag ataggttcct gttcctgtgg tttcattttt aaggatctgc tttccgatcc
     4321 acttaaagcc tgtaagcacc tcgatcagct ctgcaccgta ggctttggct accgcgtcga
     4381 tcagattggt ggatacgatg gactttacaa cctgtccgtc tgccgggatt ttgccctcag
     4441 cctgcttctg gcttaataca tattcgcaca gcagggaacc ggacatattt ccggtcagag
     4501 gaatgtattc gccggacttt gcatccttta catatacgcc caggcggtcg gcatccgggt
     4561 ctgtggcaag tacaaggtcg gcgtcctttt ccttagccag ctttaagccc agttcaaagg
     4621 cttctttggc ctcagggttt gggtagctta cagtagggaa atttccgtcc ggaagctcct
     4681 gctcgggaac tacatatacg tgagtaaagc cgatctcctt catcactctt ctggcaggaa
     4741 tatttccagt gccgtgaaga ggggtatata caatgcggat ctgatcctgc atctcatcaa
     4801 tggccttctg gtttacaacc tgagccttta cctgagcgat gtacttgtca tcgatctctt
     4861 ttccgataac ctcatacttg cccgctgcga tggcagaagc ctcgtctgtg gtctttacgc
     4921 tggacagatc ctcgatggca agaacctcct ctgtcacgcc cttgtcgtga ggcggtgtaa
     4981 actgtgcgcc gtcctcccag tataccttgt agccgttgta ctccggcgga ttatggctgg
     5041 cagtgatgtt gatgcctgcc acgcagccca gctcgcggac agcaaaggac agctccggtg
     5101 tggggcgcag ggattcaaac ttgtatgcct tgattccgtt ggctgccaga gtcatagccg
     5161 cctccatggc aaattcagga gacatattgc gggagtcata ggcaatggct acgcccttgt
     5221 ctgcagcgcc ctgcttgata atgtagtttg ccagtccctg tgtggcccgt cttactacat
     5281 aaatattcat acggttgatt cctgctccga tgatccccct aaggcctgcg gttccgaact
     5341 ccagatccat gtaaaaacgc tctttgatct cgttctcatc gccttcaatg gcctttaact
     5401 cggctttggt ggcttcatca aaatacggat tggacagcca ttcctgatag atcttcatgt
     5461 aatctttcat ttctatactc cctcctgatt tcccatgcat cgtaatacct gcatttttat
     5521 gcttttgatt atactacacc aggaaaaaaa agggaagtag atgccaagta aaacttccct
     5581 tacagcaggc gtatcttctt taccatttcg cagaattcat agttatttct tgtcttggag
     5641 aaaagatcca ggatacgctc tacggcttca tcgggcttca gggaattggt ggctttccgc
     5701 actatgtcta cggcctctgc ttcctgccgg ttaagaagaa ggtcatccct tcgggtaccg
     5761 gattttaaaa tgtcaatggc cgggaaaatc cgcttttctg acaggcggcg gtctaaaacc
     5821 agctccatgt ttccggttcc cttaaattcc tcatatacta catcatccat acggcttcct
     5881 gtatctacca gagccgtagc cagaatggta aggctgccgc cctcccgcat atttctggcg
     5941 gctccgaaga aacgcttcgg catataaaga gcagccgggt caaggcctcc tgataaggta
     6001 cggccgctgg gagctacggt aaggttataa gcccttgcaa gacgggtaat actgtctaaa
     6061 aggataatca catctctccc gtgttctacc aggcgctttg ccctttcgat caccatttct
     6121 gatacacgct tatgacggtc cggctgctcg tcaaaggtgg aatagataac ctctacgttt
     6181 ggccctacga tcgcctctcg gatgtccgtt acctcctctg gccgttcatc aatcaaaagg
     6241 atcatcaggt gcatatccgg atgatttaca gtaattgctc tggcgacctg ctttaaaagg
     6301 gtggtttttc ctgccttggg cggagaaacg atcatgcctc tctggccctt tccaatgggc
     6361 gataaaagat ccagcacacg cattgctaca gagttacgct ctccccgtgt ttccatatga
     6421 agacgctcat ttgggaaaat gggagtaaga tcttcaaaat tcggacggcg ttctgtggcg
     6481 cttaagggat atccgttcac tgttttgata taaagaagag cagcgaattt ctccgttact
     6541 gctttgatcc ttctgtttcc tacaatgata tcacctgttt ttaaattgaa ccggcggatc
     6601 tgtgaaggag ccacataaac gtcattttct cccggaagga aattttcgca gcggataaag
     6661 ccaaagccat ccggcataac ttccagaata ccgtttgcct caattccgct gtcaagctct
     6721 gccatatcct gaccggcaat gccggaagcg tcactgcgag gctctggacg ggattcgcgg
     6781 acgtctgccc gtgtctctct ggtgtcacga atgtctcctg tttccctgga ttcccggctc
     6841 tctcttgact ccctgttgtc tctggattcc cggttctcct ttggctccct gctttctgct
     6901 gaatccctgg aagcttctct ggaattttgc tgcccgtatg aagtccgggg ctgtctgaca
     6961 aaacgggttt gacgtgagga gtcacgctgt gggcgtccat cgcgcggctt tccgctgtcc
     7021 cgtgactttt cgctgtcccg cggctcaccg ttttctttgc tgctttgggg ttcacggctg
     7081 ctttgtgcct cacggccttc actggaaccg gaatccggct gcaaaacagc ttcagactgt
     7141 gcggccgcct cattttcccg gcacaaaagt tctatgatct cagcttttct gagggtgctg
     7201 atgcctttaa gcccctgaga tttcgccaat tccttgagct ccgctaaagg aagggtctgt
     7261 aatttttctc gcatatgatt ctccttaatt ttacgaacag ttgtattgat cctgaattat
     7321 ctaaatgtaa tcaaaagtgg gaatggtaca aaaccggaac ggttctgacc gtgaatgagt
     7381 ccgggcgctg atatctcaga cagaaagccc ctggaatatg cggggaaagc gccctgattg
     7441 cttatacaga cattataaac gctctcccct gtgattgtaa agtttaattt ttaagccatt
     7501 ccatatgggc tttcatctgt gtttcatatc cattgtccga cggcctgtaa aagatacggc
     7561 cctccatgcc atcaggaaga tactgctggc gtacataatg cttgggataa tcatgggcgt
     7621 agagatagcc gtctccatgt cctaaggctc ctgcgccctt ataatgcctg tcctgaagat
     7681 gcacgggaac aggcatggca cgctccgtct ttaccgcctc catggcatcc tgaatagcgc
     7741 agcaggccgc attgctcttg ggtgcacagg ccacataggt cactgcctgc gaaaggatga
     7801 tctgagcctc cggcagaccg atgcgctctg cggcctgagc ggcgcttacc gctactgtca
     7861 atgcctgggg atccgcattt cccacatctt ctgcagcgca gatcataatc cggcgggaaa
     7921 taaacttaat atcttcacct gcataaatca tccgcgccag atagtacaca gctgcatccg
     7981 gatcagagcc cctcatactt tttataaaag cggaaatggt atcataatgg ctgtctccgt
     8041 ccttatcgta gcgcacagcc cgcttttgga tgcattcctg agccgctgcc agatcaatgt
     8101 ggatcttccc gtccgggctt cggtctgtag taagtacacc cagctcgact gcatttaagg
     8161 cagctctggc atctccattg gccgcatctg ccaggaaccg ggctgcatcc ggctcgatca
     8221 ctgcatcata tgtacccatc ccccgttcca catccgtaat ggccctttgg atcagttcca
     8281 gaatatcctg cgtttccaga ggcttcagct caaaaatgcg ggatctagag ataagagcgc
     8341 tgttgacctc gaaataagga ttttccgtcg tagcgccgat aaggatcaag gtgccgtcct
     8401 ctacaaaggg aagaagataa tcctgctgtc ccttgttaaa gcggtggatc tcgtctacaa
     8461 acaaaatggt tcttttgccg tacattcccc tgctgtcctt tgcggcggcc acaatctcct
     8521 ccatatcctt ttttccggcc actgtggcgt tgatctgctt aaattcagcg ctggtggtgt
     8581 tggcaataac ctttgccagc gtggtttttc cggttcctgg cgggccgtag aaaataagag
     8641 atccaagttt gtccgctttg atggcgcggt aaaggagctt gtcctttcca atgatatgct
     8701 gctgccccac cacctcatcc aacgtccttg ggcggagacg ggaagcgaga ggcgcttcct
     8761 tttccattgt attttctctc atataatcaa acagattcat ctttatctct ctttcagtca
     8821 atcatcagtt catcggctgt aacaaaggta tatccttctc tctggaggat atcgatcagc
     8881 tccagagccg ctgacacaga tgtatcaaat atatcatgca tcaatataat atccccatcc
     8941 tcagcttctt tcaagacctt ctttacgata cgcttctggt ttctgagctt ccagtccagg
     9001 gaatccacag accagaggac tgtcgtcatg ccggactgac actccagctc ctcgttccag
     9061 tctccgtagg gcggacgcat atattgaggt cgggagcccg taatgtcctc gatcatcccg
     9121 ctggtgcggt ctaccgcctc acagacagcc gcaggacccg cttttgtaag gcgcacatga
     9181 ttatagctgt gatttccgat caggtggcct tcttcctgca tccgttttac cagagcctcg
     9241 tttcccggta tacagcttcc catgagaaaa aaggtagcag gtactccccg ttcctttaaa
     9301 ccatctaaaa gaaccggcgt atggacggga tgggggccgt catcaaaagt aagagcgaga
     9361 tatttttccg gctcaaacgt accctcaggt gctgatgcct ccctgaccgt aaccggagca
     9421 gccaaaaggc agatcagata tgccagaagg ataaaatcgg cggacagaac agccgacaac
     9481 agatggaccc tgtgtttcca cttcacattc aggcctccgg gcagattggt agattgattt
     9541 attatatgca gttccataca aaaatagaag caggccggaa gtcatgctcc cggcctgtgt
     9601 actgccttat tctttcctgt gctcctttta taacctgcct tctacactcc cggcttctcc
     9661 cccgccgcta cctgcccctg ggagggagca gagctgacac cggggccgcc gttgattcct
     9721 gtaacaggag ctgagggctc tggaacgttt tgggaggaag agctgtttcc gcttccgttt
     9781 gacgggataa taatggggtt agaggatgtg ccggagctgc cggaagccgt tcctgcactg
     9841 tcggacgcgc tctcctggtt tgtggaaccg ctttcctcct gcccctgagg aatcagatgg
     9901 ccgtctgctg ctgcctcata aacagcgccg ccgatctcca gcgtctgtcc tgcagccatg
     9961 cgtcctgtgg agggatccat ataataatgg agaccgttta cctcgatcat gcccgtggtc
    10021 atcgctccgc ttccgtcaaa ataataccag gctccatcca tctcacgcca gccggtcgcc
    10081 atcacgccgg agcccttcag ataataccgc tttccgcctt cactcagcca gccggtctgc
    10141 atcacgccgt ttgtatctga gcaataccag gagccattat cctggatcca gccctttgcc
    10201 agggcacctg atgcgtccag atagtaccat ttgccgtttt ggtttgtcca tccaaccgcc
    10261 atttttccgg aagactccat ataataccgc tttccgtctg catccagcca gccggtctgc
    10321 atgataccgt tggagtctgt ataataccag gctccattgt cctctatcca gcctttttta
    10381 acagctcctg agcttcccag atagtaccat tttccctgat cctgacgcca gccagtctgc
    10441 atctttcccg tggtctcgtc cagataataa cggctgccgg ataccgtgat ccagcccgtg
    10501 gacgcaaggc cttcgctgtc catataatac cagtctgaac tatcctgagc ccaggcgttt
    10561 ttctgtttgg catagttgct gtaataatac cgtttcccgt taatggtacg ccaggtattg
    10621 gccggaatct ggccggagaa atccttaaac tggaaatcaa tatccacatt tccattgatt
    10681 ccctttacag aaccggtgct ggtcgcctgc cacataacag ggttctggta gctgggacgg
    10741 gcagaataac gggctaccca tacgggataa tccatcaggc tcatatccag cttatttttc
    10801 agccagtttt catttgcata gacaatcgga taatatccgg aatcggatat ctttttgcag
    10861 aacgcatttg cgatcgctgc cagctcctct tttgaaaggt ttccctgagt agaatcctcc
    10921 atatcgaaag ctacaggata agaaacagga taatcatgga tcagatccag cacaaaatcg
    10981 gcctctgcct ctgccatttc caccgttgta gccagtgagt aaatatacac gcccaccttc
    11041 acgccggcag cgctggcctg ctggatattc cggtgaaaat agggatctac cgctcccttg
    11101 gaacgggttc ccagcatcac gaaggacacg tcatctgctg cgacctggct ccagttgatc
    11161 tccccctgcc atctggatac gtcaattcct ctggcaagca cattttgaat ggcacttccg
    11221 tccggcatct gatagacgcc gtttaccttt ttccatgcat ttggattggt ctggtctgct
    11281 ccttttgcat tctcagaagt gctggaggca gaaggcacat tgccggttcc tccggatcct
    11341 cctgtttctc ccgggcctcc tgcagcagac gctccaacag acgccggcat ttctcccggc
    11401 cctgcagcgt aggccgtata cgcagaccca agggttcctg cgcccagaat tccagccagg
    11461 acgagtgcct ggcagcgctt tctcatctcc tgtctctttt tcattttcct ctccttatat
    11521 cgtactgata gcaaaaacgg aggatgctgt cagccagtcc cgccgcagca tcccccctta
    11581 tcaacttgtc cttgtcacat gtttccatat cggatttctc ttatcagcct ggtccatctg
    11641 tgcgtccggc ttccagagag ccgctgctgg aaggaccgct ggagctggtg gttccggagc
    11701 tgctggaacc tggaccagat gagctggaac tgccagaagt ttttgatacg ccggggccgc
    11761 cgttttcacc ggttacgccg cctccggaag actggtttcc tgcagccggc gtctgtgcag
    11821 atacgccgtt tgcattctgg catacgccgg atgcgtcaaa attgtaggag gtaccgttga
    11881 tatttaaggt agttcctgct gtcatcacgc cgccgtctgc cggatttaag taataatact
    11941 tgctgtcgat caggatccag cctgtcatca ggtgaccgtt gctgttgaag tagtaccact
    12001 tgccgtcgat ctgcttccag cctacagaca tctttccgtt tgtcatatcc atataatact
    12061 tgctgccctc gctgtcgctg agccagccga tgagcatacg tccgtctgta ttcagataga
    12121 agtaatcctg gtctaccttt gcccagccgg tcatcatctt tccgtctgtg ccgaacagat
    12181 accagttgtc ctgaatctga gcccatgcgt tcacaacaca cttgccgttt tcctggaaat
    12241 aataccaggc tccgtccatc tcacgccagc cggttcccat gctgccgtcg ccgcgcataa
    12301 aatagtaatc atcgccaata cgcagccagc ccgtaaccat ggtgccgtcc tccagcatat
    12361 agtaccattt gccgtattcc ggattgatcc agccctttgc catcaggccg cccggacgga
    12421 agaaatacca ttttccatca atctgcttaa aaccagtggt catatagccg gtagaaagat
    12481 ccatatagta ataaccctcg ctggtctgaa gccagcctgt gtgggtcttt ccctggttaa
    12541 tataggtcca gtgatcgttt gtcttggtcc agccgtctgc cgcataggat gtaaccgcca
    12601 ttcccagggc catggcgctg atcacgccgg ctgcggtcaa agccgccaga catttcttac
    12661 gtttcatgcc ttttcctcct cattcttctg tatataagcc cctttgtctg tttcgggttt
    12721 tcatcctttt aattatagca cagattatct gtaaggaagg taacaatttt attacaattt
    12781 ccgtaaattt tcatacctgc tacagagcct ttaatttttt ataatagccc tttgcatacc
    12841 ggtacatgat gatagtatat tctccaaatt cctctcctaa agctgccttg tatacagccc
    12901 acaaacacca gaggaaaccg cccagggccg catttgcaaa tacggaataa tattcttctt
    12961 ccgaaggctt tctctctaaa taaatctcca gaagcttttc tgtctgttct gcgtcatagt
    13021 acgaatagat cgccgacatg gaaatgtcca taatgggatc gcacattccc gcatattccc
    13081 aatccagcag ttttgcactg ccgtcttcaa acatcaggaa gttatccaca tttgcgtcaa
    13141 tatggcagag acattgtgga cgatccatct gatccaaacg atccatcagc tcattcatcc
    13201 agcctctgac cttctcataa tcttcaaaag ggattcctcc gtgctgcagg cagagccttt
    13261 cataaaaatc tatccgttcc cgaatatcaa aggaatgatc gactgtaaga ccagactggt
    13321 gaagctttct tacaataccc atacagattt ccatatcttt ccagttatct gccgctgcat
    13381 tccgggcgtt ttcatagtac cgtgcaatct tatagccggt atccgggttc atgtacagca
    13441 cttcttccgt aatccccaga gaggctacgg tatcataaac ctctttttcc tccctgcggt
    13501 tgattaaaag ctctgtcccc ggccccggaa tcctgcagat gcagtgagcg ccatccaccc
    13561 ggaacaagaa ggatttattt gtcattccgg ctttcaggca gcggatatct gtaatatggg
    13621 actcaggcac cttaaatacc ctggatacca gatccattgc cgcattatcc gaatggttct
    13681 gatagcgttc atcaaacagg cgcagctcct ccagattttc aaattcatac acctggtttt
    13741 caggctgacg gtttacatcc atctcgatct gatcccactg agacgccttc cagccggaag
    13801 cctcttctgc gcgggcaaga agcgttcccc cttctgcctc cagacggcgc tttgcctctc
    13861 ccttgagcat atctacaaag ggctgctccc agtaaaactg ttccgttccc ggagtttcat
    13921 agtaggcttc taataccgga aggaaccggg ctgaaaattc tctggacaga tagaccgggc
    13981 cgtacatcac ccactgatcc cttccgccta catttacatc tgtaatcctt ccctttttag
    14041 taaaggtaaa gcaccactcc ttggtatccc ccttctggaa gga
//
LOCUS       DBMDUH010000059        14013 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_2352_length_14013_cov_8.357605, whole genome shotgun sequence.
ACCESSION   DBMDUH010000059 DBMDUH010000000
VERSION     DBMDUH010000059.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 14013)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14013)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14013
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2352_length_14013_cov_8.357605"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /gene="trmB"
                     /locus_tag="ACZPCG_06635"
     CDS             complement(<1..474)
                     /gene="trmB"
                     /locus_tag="ACZPCG_06635"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HIR1284777.1"
                     /translation="MRLRHIPGAEETIASSSYVIQEPALHKGSWNQVFGNDHPIEIEV
                     GMGKGRFIMELAQKNPHINYIGIERYSSVLLRGLQKRAGLELDNIYFLCIDAKAMADY
                     FAPGEVNRIYLNFSDPWPKDRHARRRLTSPDFMAVYDQILARDGVIEFKTDNRGLF"
     gene            784..1806
                     /locus_tag="ACZPCG_06640"
     CDS             784..1806
                     /locus_tag="ACZPCG_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR1284778.1"
                     /translation="MSNLEHTTSWQQIQQGVKEAERLIVNKNYNLVMVKARQTLECMV
                     RCMAEKACLVEGDLSDTIDQLYEGGWINKTTKDNYHTIRILGNKAVHENDNTAYNANQ
                     AYQLLTQEVHAFSHDGGASGGGRTQRISSGGGRERTGQRGSQRPASAGNRAGGSRPVQ
                     GQRTQTGHSGYSSGQRNPNVRRRKRRGVSPLFYLWRLLIPLLVVILLIVVIQMIRPGK
                     DEVKDTAAPTESSQTVESMPETEPETEPETQPEAERYVVTGNSVNVRTEPSTSSRILV
                     QLSQGTEVDYVKRYNNDWTVINYDGQEAYVSSQYIEKKASESEPSSEEDAESASGTDA
                     AEVQAQ"
     gene            1990..2301
                     /locus_tag="ACZPCG_06645"
     CDS             1990..2301
                     /locus_tag="ACZPCG_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HIR1284779.1"
                     /translation="MDKIIDRLSDIEKAAGSLMDDAGVRKKALAKEMEEKTAAFDREL
                     EQKTAARISCIQKQMEQEMQEELDKQAADARGMIARLEETYEKQHKLYAESLFRSMIK
                     E"
     gene            2306..3328
                     /locus_tag="ACZPCG_06650"
     CDS             2306..3328
                     /locus_tag="ACZPCG_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006778168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000220 - vacuolar proton-transporting
                     V-type ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V0D/AC39 family V-type ATPase subunit"
                     /protein_id="HIR1284780.1"
                     /translation="MGSLIHYSGISTKIRAMERWRIKPEQFEQMAALESVPEAVRFLR
                     SLPPYEKIFSGVEDKELHRGRIEQLLNLAQYEDFASLYQFANMKQRRFLDLYFMHYEI
                     GIVKTCLRNGAGRREAAQDLSGFKEFFDRHSSVDLAQLSQCRSIDEVISGLKGTIYYS
                     PLESLRQKGQAVLPACETAVDMLYFKTVWKIKEKYLSGQEKKDLTQCFGTRMDMLNLQ
                     WICRAKKYYHLPEGEIYAMIIPISLHLKKTEIRAMAQAEDVEQVYALIRNSWYGRLDL
                     GNLEKGQSLEEPCREVIDRIYELTSRKEPYSASVLNSYLYFKEREIEKIITTIERIRY
                     GVAAGI"
     gene            3344..5278
                     /locus_tag="ACZPCG_06655"
     CDS             3344..5278
                     /locus_tag="ACZPCG_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006778167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HIR1284781.1"
                     /translation="MIEKMKFLSITGPREDIDRVADTYLSRYEIHLENALSELKTVKN
                     LRPYIEVNPYRDQLAMARSLMERFGGQLPEVKRSGEETLPDIQEASAFVERLSEELKE
                     LTDRREELLIQIEALKKSKDRVAPFTELNYSLREILGFKFIKFRFGRVTREYYDKINN
                     YVYDTIDTVMFKCLEDSEYVWFVYFVPDKLSEKIDAIYASMHFERVFLPDEYEGTPLE
                     AEHVLEEKIQALEAEKLEAEQAIKTILEDKKEELTKAWMRLERFGINFDVRKLAACTM
                     HNSNNFYILCGWMSESDARALRKEIEGDENTFCIVEDNRGNITSTPPTKMKNPGLFKP
                     FEMYVEMYGLPSYNEIDPTILIGITYSILFGFMFGDAGQGLCLLIGGFLLYRFKKVRL
                     AGIISCCGFFSTIFGFLFGSIFGFEDIIDAIWLRPQEAMTDLPFIGRLNTVFVVAIAI
                     GMGIILLCMILNIINSARVHDTEKLWFDTNGAAGLVFYAALASVIVLYMSGKALPATI
                     VLVIMFVIPLAVIFFKEPLTAMVEKKSEKISGGVGMFITQGIFELFEVLLSYFSNTLS
                     FVRVGAFAVSHAAMMQVVLMLAGAEAGDPNWGIVIGGNLFVCGMEGLIVGIQVLRLEY
                     YELFSRFYRGSGRAFEPYGR"
     gene            5317..5757
                     /locus_tag="ACZPCG_06660"
     CDS             5317..5757
                     /locus_tag="ACZPCG_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002571093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HIR1284782.1"
                     /translation="MSLLVKITLSAALILSIGVPFGAFAAGEKTKGRYKTALGANLFL
                     FFGTMVIAAVLMFNGNAFAAEEAASAVGSASSAAGMGYLSAALATGLSCLGGGIAVSA
                     AASAALGAISEDSSILGKSLIFVGLAEGVCLYGLIISFMILGKL"
     gene            5757..6065
                     /locus_tag="ACZPCG_06665"
     CDS             5757..6065
                     /locus_tag="ACZPCG_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HIR1284783.1"
                     /translation="MKMYLISDNIDTWTGMRLAGVEGAVVHEREELKQELDKVLADKD
                     IGIILLTEKFGREFPEIIDNVKLERRLPLIVEIPDRHGTGRKPDFITSYVNEAIGLKL
                     "
     gene            6113..6694
                     /locus_tag="ACZPCG_06670"
     CDS             6113..6694
                     /locus_tag="ACZPCG_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HIR1284784.1"
                     /translation="MTTEEKLKHFQEICMTDAREKSARMLDDCVRALDAAFEEHKAEA
                     ERRAAMQEEAQKEKLEREKNKKLSIGQLDLKREISHRQEELKDKLFVEVKDMLANFME
                     TREYQELLERQIRSAKEFAGDEALIVYMDPSDEDKARRLALHNNVTVKISEYSFSGGI
                     RAVVPSKHVLIDHSFDTKLKEARHDFTFHLGGK"
     gene            6695..8461
                     /locus_tag="ACZPCG_06675"
     CDS             6695..8461
                     /locus_tag="ACZPCG_06675"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008720832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HIR1284785.1"
                     /translation="MTKTGVIYGINGPVVSLEGDPGFQMNEMVYVGKENLVGEVIGLT
                     SQKTTIQVYEETSGLKPGEVVTGTGAPVSVTLAPGILSNIFDGIERPLSKIAEHSGYS
                     ISRGISVDSLDTSKKWDTHITVKKGERLMPGTIIAEVPETRAIVHKVMVPPDMEGYVI
                     DVVPDGSYTINDMLVKVQLMDGSEKELTMTQKWPIRVPRPAAKRFAATRPLITGQRIL
                     DTMFPLAKGGTAAIPGGFGTGKTMTQHQIAKWSDADVIIYIGCGERGNEMTQVLEEFS
                     ELIDPKSGNPLMDRTTLIANTSNMPVAAREASLYAGLTLAEYYRDMGYHVAIMADSTS
                     RWAEALRELSGRLEEMPAEEGFPAYLASRLSAFYERAGMMRNLNGTEGSVTIIGAVSP
                     QGGDFSEPVTQNTKRFVRCFWGLDKSLSYARHFPAIHWLTSYSEYVNDLSSWYMDNVD
                     QKFVDYRNRLMALLTQESSLMEIVKLIGSDVLPDDQKLVLEIARVIRLGFLQQNAFHK
                     EDTSVPLKKQFKMMEIILYLYKKSKTLISMGMPMSVLKEDPIFDRIISIKYDVPNDRL
                     DLFDEYKTQVDRFYENVVERNA"
     gene            8473..9849
                     /locus_tag="ACZPCG_06680"
     CDS             8473..9849
                     /locus_tag="ACZPCG_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008976939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HIR1284786.1"
                     /translation="MAVEYLGLSEINGPLVVLEGVQNAAYDEIVEMTVAGNTKKIGRI
                     IEIYGDKAIIQVFEGTDGMALRNTHTRLTGHPMEIAVSEEMLGRTFNGIGEPIDGLGD
                     IISDVRLDINGQPLNPVSREYPRNYIRTGISAIDGLTTLIRGQKLPIFSGNGLPHDQL
                     AAQIVQQASLGEGSDESFAIVFGAMGVKHDVADFFRRTFEESGVSEHVAMFINLANDP
                     VVERLITPKIALTLAEYLAYEKGMHILVILTDMTSYAEALREVSSSKGEIPSRKGFPG
                     YLYSDLASLYERAGIVAGRHGSVTQIPILTMPNDDITHPIPDLTGYITEGQIVLDRQL
                     NSQSVYPPISVLPSLSRLMKDGIGEGYTRADHQDVANQLFSCYAKVGDARALASVIGE
                     DELSPLDKKYLEFGREFESRFIGQTHYENRFMTETLDIGWELLRMLPREELDRIDTKV
                     LDQFYEKR"
     gene            9861..10484
                     /locus_tag="ACZPCG_06685"
     CDS             9861..10484
                     /locus_tag="ACZPCG_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005539076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HIR1284787.1"
                     /translation="MDPNTFPTKGNLIAAKNSLKLATMGYGLMDKKRNILIRELMGLI
                     DEAKGIQSEIDAVFTAAYQALQEANIELGINYVQEIGMSMPVDDGVGIKARSIMGTEI
                     PLVRHEQGPMKVAYGFNNTSESLDIARQHFERVKELTIKLSMVENSAYRLANSIKKTQ
                     KRANALKNITIPHYEALSRTISNALEEKEREEFTRLKVIKRMKAEKP"
     gene            10628..10903
                     /locus_tag="ACZPCG_06690"
     CDS             10628..10903
                     /locus_tag="ACZPCG_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008720828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIR1284788.1"
                     /translation="MNKTEFIAAVAEKAEISKKDAEKAVKAFTDVVTEELVNGGKVQL
                     VGFGNFEVAERPAREGRNPKTGEAMTIAASKTPKFKAGKALKDEVNK"
     gene            10907..11146
                     /locus_tag="ACZPCG_06695"
     CDS             10907..11146
                     /locus_tag="ACZPCG_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002605178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HIR1284789.1"
                     /translation="MRLDKFLKVSRLIKRRTVANEACDAGRVLVNDKPAKASLNVKTG
                     DIIEIQFGAKAVRAEVLDVQETVKKDEAKELFRYL"
     gene            11260..11547
                     /gene="yabP"
                     /locus_tag="ACZPCG_06700"
     CDS             11260..11547
                     /gene="yabP"
                     /locus_tag="ACZPCG_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003504842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HIR1284790.1"
                     /translation="MEETLGSIRPHRITIQDRKTAGITGVSDCVSFDENEVVLDTDMG
                     LLTIRGKELHVNRLTLEKGEVDLDGTVESFAYSSNEALRRSGESLFARLFK"
     gene            11564..11908
                     /gene="yabQ"
                     /locus_tag="ACZPCG_06705"
     CDS             11564..11908
                     /gene="yabQ"
                     /locus_tag="ACZPCG_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007719734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0034301 - endospore formation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex biosynthesis protein YabQ"
                     /protein_id="HIR1284791.1"
                     /translation="MSERIRYELLLTGLSLVMGGWLMIVYDSLRLLRLLIHHNSLFMG
                     MEDFFYWIYAGGAVFMLLYEQNDGSFRFYAVAGVFLGMAAYDRFVSRIFFRCLKKFSR
                     WFKITRKGNSGS"
     gene            11933..12250
                     /locus_tag="ACZPCG_06710"
     CDS             11933..12250
                     /locus_tag="ACZPCG_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator"
                     /protein_id="HIR1284792.1"
                     /translation="MAYYGRSKRLRRGKWENRMAIIGITMVVISLAVAVNAKGASLKQ
                     ADLDYRQKEADLARQVQDEENRTRELEEYKIYVQTKQYAEEVAKEKLGLVNPDEILLK
                     PRQ"
     gene            12392..13807
                     /locus_tag="ACZPCG_06715"
     CDS             12392..13807
                     /locus_tag="ACZPCG_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003504845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HIR1284793.1"
                     /translation="MLSGRREVRSRSDVNYYTARKLEDMARSMERLAKAFDEGMHKTG
                     SLTRDDGLAAMQTSASMVCQDCSQCGIYAESEREDSYYLYYLLRAFEQKGQIEKEDMP
                     RPFLAGCRKKEDYLAQLNRSLARAAMNLSWKNRFLESRDAVVSQFRELSLILGEFSHQ
                     IDQAADITEEYGYIMKKLFRRCHVAVENMLILEYESGRREAYVTARTTNGRCMTAKDA
                     SELMSEVIPGTRWNPAKDSRSIITRQSGTVRFEEDGEYQLLYGAARVPKQGERCSGDN
                     YTFCESPGSQAMISLCDGMGCGEPACEESGQVVELTENLLEAGFGSRAAFKLVNTVLL
                     LAGTEQHPAALDMSCVDLYTGVLDVMKLGAAPTFVLGQEGAEILEAGQVPAGILSEAE
                     PVMLSRKLWDGDHIIMVTDGVLDALPGEDKEQAMCQFLESLDFMPPQEMAERILEFAL
                     SFVPGARDDMTVLTAGIWKKE"
     gene            13820..>14013
                     /locus_tag="ACZPCG_06720"
     CDS             13820..>14013
                     /locus_tag="ACZPCG_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP-loop family protein"
                     /protein_id="HIR1284794.1"
                     /translation="MDKLTERVRKTIKEHHMVKPGDRVMAALSGGADSVCLLRILVQL
                     RDILGIRLRAVHVHHGLRGE"
BASE COUNT         3753 a         2884 c         3996 g         3380 t
ORIGIN      
        1 gaaaagtccc cggttgtctg ttttaaactc gatcacgccg tcgcgggcaa gaatctggtc
       61 atagacagcc atgaaatcgg gagaggtaag gcggcgtctg gcgtgacgat ccttaggcca
      121 cggatctgag aaatttagat agatgcggtt tacttcgccg ggagcgaaat agtcagccat
      181 ggcctttgcg tcaatgcaga ggaaataaat gttatccagt tccagtccgg ctcttttctg
      241 caggcctctc agaagtacgc tggaataccg ttcgattcca atgtagttga tatggggatt
      301 tttctgagcc agctccatga taaagcgccc ttttcccatt cctacctcta tttcgatggg
      361 atggtcattt ccgaatacct ggttccagct tcctttgtgg agggccggtt cctggatcac
      421 ataggaactt gaggcgatgg tttcctctgc cccgggaata tgtcttaaac gcatagatgt
      481 atgtcctcgt ctttctttgc ggtcaaaccg cagggaagtg tagttgtttt atccgcagac
      541 tgcggttttt gtaaggatga tcggacttat tttaacattc aaatggggga aatgcaactg
      601 tgaaacgcag gcggaagcct gtcaaaaacg agaaaaacgg tttcaaggta tatttttagg
      661 atatgatcat atagttcctt cataaaatcc ttccaattcc tagtgcaatt tcaatataaa
      721 taaggtataa tatgaacaca gagagtttct gtagaaatct gctgatacaa aggaggaagg
      781 gatatgtcaa acctggagca taccaccagc tggcagcaga tccagcaggg ggtcaaagag
      841 gcagaacgtt tgattgtgaa taaaaattat aatctggtga tggtaaaagc aagacagaca
      901 ttagagtgca tggttcgctg tatggcggag aaggcctgtc tggtggaggg cgatctgtca
      961 gataccattg accagctgta tgaaggcggc tggatcaata agacgacaaa agataattac
     1021 cataccatcc gcattcttgg gaataaggcg gtgcatgaaa atgacaatac agcgtataat
     1081 gccaatcagg cataccagct tttaacccag gaggttcatg ctttttctca tgatggcggc
     1141 gcttccggag gggggcggac ccagagaata tcttccggag gaggccggga acgtactgga
     1201 cagaggggaa gccagaggcc tgcatctgct ggaaaccgtg cgggaggcag ccggcctgtc
     1261 caggggcaga gaacccagac cggacattcc ggttacagct ccggacagcg aaatccaaat
     1321 gtcagacgga gaaaacgcag gggagtatca ccgctgtttt atctgtggcg tcttctgatt
     1381 ccgctcctgg tggtgatcct tctgatcgta gtgatccaga tgatccgtcc cggaaaggat
     1441 gaggttaaag atacggcagc tcccactgaa agctcacaga cggttgagag tatgccggaa
     1501 acagagccgg agacagaacc ggagacgcag ccggaggctg aacgttatgt agtgacaggc
     1561 aacagcgtaa atgttcgtac agagccgtcc accagcagcc gtattctggt acagctttct
     1621 cagggcacag aggtggatta tgtaaagcgt tataataatg actggactgt gattaactat
     1681 gacggacagg aggcttatgt ttccagccag tacattgaga aaaaggcttc ggagagtgag
     1741 ccttcttccg aagaggatgc agaaagcgcc agtggaacag acgcagcaga agttcaggct
     1801 cagtagcaga aaatttccag atatttgaaa ttaaaggaga tatgtcattc atggaaaagg
     1861 gagggcatat cttttttatt gtattgttct gaaaaaaaat ttgttaaaaa tttatttatt
     1921 ctacaaaatg ccactggtaa tttcagaaaa aaagagtatt ataagggaat ataaaaagga
     1981 ggcgtaaaaa tggacaaaat aattgaccgg ttatctgaca ttgagaaagc cgccggatct
     2041 ttaatggacg atgcaggagt ccgaaagaag gctcttgcaa aggaaatgga agagaaaacg
     2101 gcggcttttg acagagaact ggagcaaaaa acagctgcgc gcatttcatg catccagaag
     2161 cagatggagc aggagatgca ggaggaactg gataagcagg ccgctgatgc caggggcatg
     2221 atcgcccgtt tggaggaaac ttatgagaaa cagcataaat tgtatgcaga gagcctcttt
     2281 cggagcatga taaaggagtg agcctatggg aagcttgatt cattacagcg gcatttctac
     2341 caagataagg gctatggaac ggtggcgtat taagccggaa cagtttgaac agatggccgc
     2401 tttggaaagc gttcctgagg cagtccgttt tcttcgctcc ctgccgccgt atgagaagat
     2461 tttttccggt gttgaggata aagaactcca cagggggagg attgaacagc ttttgaacct
     2521 ggcccagtac gaggactttg caagtctgta tcagtttgcc aatatgaaac agaggcggtt
     2581 tttagacctg tatttcatgc attatgaaat agggatcgta aagacctgcc tgagaaacgg
     2641 agccggacgc cgtgaggccg ctcaggatct gtcgggtttt aaggagtttt ttgaccgcca
     2701 ttcttctgtg gatctggccc agctttctca gtgccgttct attgatgagg tgatttcggg
     2761 tctgaagggt acgatttact acagtccgct ggaaagcctg cggcagaagg gacaggcagt
     2821 tcttccggcg tgtgagacag cggtggatat gctgtatttt aaaacggtgt ggaaaataaa
     2881 ggaaaaatat ctttctggac aggaaaaaaa ggatctgacc cagtgcttcg gtacccggat
     2941 ggatatgctg aacctgcagt ggatctgccg ggcaaagaag tattaccatc tgcctgaggg
     3001 tgaaatttat gcaatgatca ttccgatcag ccttcatctg aaaaaaacag agatccgggc
     3061 tatggcccag gcagaagatg tggagcaggt ttacgcgctg atccgaaaca gctggtacgg
     3121 acggctggat ctgggaaacc tggaaaaagg acagagcctg gaagaaccct gcagggaagt
     3181 gatagaccgt atttatgagc ttaccagcag gaaggagcct tattctgcgt ctgtactcaa
     3241 ttcatacctg tattttaaag agagggagat tgagaaaatc attactacca ttgagcggat
     3301 ccgctacgga gtggcggccg gcatataaag ggggttttaa aacgtgatcg aaaagatgaa
     3361 gttcttaagt attaccggcc ccagagagga tattgaccgg gtggcggata cctatctgtc
     3421 ccgatatgag atccacctgg aaaatgctct ttctgagctg aaaacggtaa aaaacttaag
     3481 accgtatatt gaagttaatc cgtacaggga tcagctggcc atggccagaa gcctgatgga
     3541 gcggtttgga ggacagctgc cggaggtaaa acggtccgga gaggaaactc tgccagacat
     3601 tcaggaggcg tccgcttttg tggagcgctt aagtgaagag ttaaaggaac tgacagaccg
     3661 cagagaggag cttttgatcc agatcgaggc attgaaaaag tccaaggatc gggtggctcc
     3721 ctttacggag ctgaactaca gcctgcggga gatcctgggc tttaagttta tcaagttccg
     3781 cttcggacgc gttaccagag agtattatga taagatcaat aattatgtat atgatacaat
     3841 agacaccgtt atgtttaagt gtctggagga ttcagaatat gtctggtttg tatattttgt
     3901 accggacaag ctgtcggaaa aaatcgacgc gatctatgct tctatgcatt ttgagcgtgt
     3961 gttcctgccg gatgaatatg aaggcacgcc gctggaggca gagcatgttc tggaagaaaa
     4021 gatccaggca ttggaggccg agaagttaga ggcagaacag gcgataaaga cgattctgga
     4081 ggataaaaaa gaagagctta caaaagcatg gatgcggctg gagcgctttg ggatcaattt
     4141 tgatgtgcgc aagctggcag cctgcaccat gcacaattcc aataacttct atattctctg
     4201 cggctggatg tccgaaagcg acgccagagc cctcagaaag gaaattgagg gggatgagaa
     4261 taccttctgt attgtagagg acaaccgggg aaatattacc agcacccctc cgaccaagat
     4321 gaagaatccg ggactgttta agccctttga aatgtatgtg gagatgtatg gacttccttc
     4381 ctacaatgag atcgacccga cgatcctcat tgggattact tattccattc tgtttggatt
     4441 tatgtttgga gatgcgggac agggactctg tcttctcata ggaggctttc ttttgtatcg
     4501 atttaagaag gtgcgtctgg cgggaattat ttcctgctgt ggcttcttct caacgatttt
     4561 cggcttcctt ttcggcagca tcttcggttt tgaggatatc atagacgcca tatggctgcg
     4621 tcctcaggag gcaatgacag acctgccctt tatcggccgt ctgaacaccg tgtttgtggt
     4681 tgctattgcg atcggtatgg gtattattct tttgtgcatg atcctcaata tcataaacag
     4741 cgccagagtc catgatacgg aaaaactctg gtttgatacg aacggcgctg cgggccttgt
     4801 cttttatgcg gctctggcaa gcgtgatcgt gctgtatatg agcggaaaag cccttcctgc
     4861 tacgattgtt ctggttatta tgtttgtgat ccctctggct gtgatattct ttaaggagcc
     4921 tctgacagct atggtggaaa agaaatccga gaagatatcc ggcggtgtgg gaatgtttat
     4981 cactcagggt attttcgagc tctttgaagt gcttttaagc tatttttcca acacactttc
     5041 ttttgttcgt gtgggcgcct ttgcagtgag ccatgcagcc atgatgcagg tggtgctgat
     5101 gcttgctggc gctgaggcgg gagatcccaa ctggggcatc gtcatcggcg gcaacctctt
     5161 tgtctgcggt atggagggac tgatcgtggg gatccaggtg cttcgtctgg agtattatga
     5221 gctgttcagc cgtttctacc ggggcagcgg acgggcattt gagccttatg gaagatagga
     5281 aaatgtaaaa aattacaata taaggagaac aaaattatga gtttattagt taagatcaca
     5341 ttgagcgcag cacttatttt aagtatcgga gttccctttg gagcatttgc ggcaggagag
     5401 aagacaaagg gacgctataa gacagctctg ggagcaaatc tgtttctttt cttcggcacc
     5461 atggtgatcg cggctgtact gatgtttaac ggaaatgcat ttgcggcaga ggaagcggct
     5521 tccgcagtcg gttcagcgtc atctgcagcc ggtatgggtt acttatctgc agctcttgca
     5581 accggtcttt cctgtctggg cggcggtatt gcagtatctg ctgcagccag cgcagcgtta
     5641 ggagctatca gtgaggacag ctcgattctt ggtaaatcct taatcttcgt aggtcttgcc
     5701 gaaggtgtgt gtctgtacgg tctgatcatt tcctttatga tccttggtaa gctgtaatga
     5761 aaatgtatct gatcagcgac aacatcgaca cctggaccgg catgaggctg gcaggtgttg
     5821 aaggggctgt ggtacatgag agggaggaac tgaaacagga actggataag gtcctggccg
     5881 ataaggatat aggcattatc ctgctgacag agaagttcgg cagggaattt ccggagatca
     5941 tcgataatgt aaagctggaa cgcaggcttc cattgatcgt ggagatcccg gatcgccatg
     6001 gtaccggccg caagccggac tttatcacct cttatgtaaa cgaagccatt ggactgaagc
     6061 tgtagggcgc agccgcccag gcacattttt tcagagaaag caggtgaatc gcatgacaac
     6121 agaagaaaaa ctgaagcatt ttcaggaaat ctgcatgaca gatgccagag aaaaaagcgc
     6181 ccgtatgctg gatgactgcg tccgggctct ggacgcggcc tttgaggagc ataaggcaga
     6241 ggcagagcgc agggctgcca tgcaggagga ggcccagaag gaaaagctgg agcgtgaaaa
     6301 aaataaaaag ctttccatcg gacagctgga tttaaagcgt gaaataagcc acagacagga
     6361 ggagcttaag gataagctgt tcgtggaagt aaaggatatg ctggcgaatt ttatggaaac
     6421 aagagagtat caggagcttc tggaacggca gatccggagc gctaaggaat tcgcaggaga
     6481 tgaggcgctg atcgtttata tggatccctc tgatgaggat aaggcccgca gactggctct
     6541 ccacaataac gtgacggtaa agatcagcga gtattccttc agcgggggga tccgggctgt
     6601 ggtcccgtcc aagcatgttc tgatcgatca ttcctttgac accaagttaa aggaagccag
     6661 acatgatttt acatttcatt tgggaggtaa gtaaatgacg aagacaggtg tgatctacgg
     6721 catcaacggc cctgtggttt ctctggaggg agatcccggt ttccagatga acgaaatggt
     6781 gtacgttgga aaggaaaatc tggtaggcga agtcatcgga cttacctcac aaaagacaac
     6841 gatccaggtc tatgaggaga ccagcggctt aaagccggga gaggttgtca ccgggacggg
     6901 agcgcctgtg tccgttaccc ttgctccggg tattctaagt aatatttttg atggaattga
     6961 gcgaccctta agcaagatcg cagagcacag cggttactcc atcagccggg gcatcagtgt
     7021 ggactcgctg gatacttcca aaaaatggga tacacatatt acagtgaaaa agggcgaacg
     7081 gctgatgccg ggaaccatca ttgcagaggt gccggagaca agagcgatcg tacacaaggt
     7141 catggttccg ccggacatgg agggctatgt cattgacgtg gttccagacg gatcgtatac
     7201 gatcaatgat atgcttgtaa aagttcagct gatggacggt tctgagaagg agctgaccat
     7261 gacccagaag tggcccatcc gtgtgccaag gcccgctgcc aagcgttttg cggctacccg
     7321 ccctctgatt acgggacagc gtatcctgga taccatgttc cctctggcaa agggaggtac
     7381 ggctgccatt cccggcggtt tcggaaccgg aaagacaatg acacagcatc agatcgccaa
     7441 gtggtccgat gcagatgtga tcatctatat cggctgcggc gagcgcggaa atgagatgac
     7501 tcaggtattg gaggagtttt cagaactgat cgatccaaag agcggaaatc ctctgatgga
     7561 ccgcaccaca ctgatcgcca atacttccaa tatgcctgtg gcggctcgtg aggcaagcct
     7621 gtacgccgga ttgaccctgg ctgagtatta cagggatatg ggatatcatg tggctattat
     7681 ggccgattct acctcccggt gggctgaggc gctcagagag ctgtccggcc gtctggagga
     7741 gatgcctgca gaggagggat tcccggctta cctggcatcc cgtctgtctg ctttttatga
     7801 gagagcaggc atgatgcgca atttaaacgg aacagaaggc tctgtaacga ttatcggagc
     7861 tgtgtcaccc cagggtggcg atttctccga gcctgtgacc cagaacacca agcgttttgt
     7921 gcgctgcttc tggggactgg ataagagtct ttcctatgca aggcattttc cggcgatcca
     7981 ttggcttacc agctattccg agtatgtaaa tgatttgtcc tcctggtata tggacaacgt
     8041 agaccagaag tttgtagatt accgcaaccg tctcatggct cttctgacac aggagagcag
     8101 cctgatggag attgtaaagc tgattggttc tgatgttctg ccggatgatc agaagctggt
     8161 tctggagatt gccagagtga tccgtctggg ctttctgcag cagaatgcct tccataaaga
     8221 ggatacctct gtacccctta agaagcagtt taagatgatg gagattattt tatatttgta
     8281 caagaagtca aagaccctga tttccatggg tatgccaatg tccgtcctaa aggaggatcc
     8341 tatttttgac cgtatcattt ccatcaagta cgatgtacct aatgaccgtc tggatctgtt
     8401 tgacgagtat aagacccagg tagaccggtt ctatgaaaat gtagtggagc gcaacgcata
     8461 aggaggattc agatggcagt tgaatattta ggtttaagtg agatcaatgg ccctctggtg
     8521 gttctggagg gcgttcagaa tgcggcatat gatgagatcg tggaaatgac cgtagccgga
     8581 aacacgaaaa agatcggccg tattatcgaa atatatggag ataaggcaat tattcaggtt
     8641 tttgagggga cagacggtat ggctctgcgg aatacccata caaggcttac gggacatcct
     8701 atggagattg ccgtgtctga ggagatgctg ggacggacct ttaacggaat cggagaaccc
     8761 attgacgggt tgggagacat tatttccgat gtgcgtctgg atatcaacgg acagccctta
     8821 aatcccgttt ccagagaata tcccagaaac tacatccgca caggcatctc cgccattgac
     8881 gggcttacga ccctaatccg gggacagaag ctgccgatct tttcaggaaa cggccttccc
     8941 catgaccagc tggcagccca gatcgtacag caggcgtctc tgggagaagg ttcggatgag
     9001 tcctttgcca ttgttttcgg agctatgggc gtaaagcatg acgtggcgga tttcttccgc
     9061 agaacctttg aggagagcgg cgtttccgag cacgttgcca tgtttatcaa tctggcaaac
     9121 gatccggtgg tagagaggct gatcactccc aagatcgccc tgactctggc agaatacctg
     9181 gcctatgaga aggggatgca tattctggtt atcctgacgg atatgacctc ctatgcggag
     9241 gcgctgcgtg aggtttcctc atccaagggt gagatccctt ccagaaaggg attcccaggc
     9301 tacctctaca gtgatctggc ttctctttac gagagagcgg ggattgtggc aggccgtcac
     9361 ggctctgtga ctcagatccc tattttgact atgcccaatg atgatattac ccatcccatc
     9421 cctgacctga cgggatatat cacagaaggg cagatcgttt tggaccggca gttaaacagc
     9481 cagtctgtat atccgcccat cagcgttctt ccgtccctgt cccgtctgat gaaggacggc
     9541 atcggagagg gctataccag ggcagatcat caggatgtgg caaaccagct tttctcctgc
     9601 tatgccaagg tgggagacgc aagagcgctg gcttctgtaa tcggtgagga cgagctgtcg
     9661 cctctcgata aaaagtatct ggaattcgga cgggagtttg agagccgttt tatcggccag
     9721 acccactatg agaaccgttt catgacagaa accctggata tcggctggga gcttcttcgt
     9781 atgcttccga gagaggaact ggaccgtatt gatacaaagg ttctggatca gttttacgag
     9841 aaaaggtgag gtgacgcagc atggatccga atacatttcc caccaaagga aatctgatcg
     9901 cggctaaaaa ttccctgaag cttgccacta tgggctatgg tcttatggat aaaaagagaa
     9961 atatcctgat ccgggagctg atggggttaa ttgatgaggc aaagggaatc cagtcggaga
    10021 tcgacgctgt gtttacggct gcttaccagg ctctccagga agcgaatatt gagcttggga
    10081 tcaactatgt ccaggagatc ggcatgtcca tgcctgtaga tgacggtgta gggatcaagg
    10141 cacgaagtat tatgggaacg gaaatccctc tggtgcgtca tgaacagggg cctatgaagg
    10201 tggcttacgg ttttaataac acttcagagt ccctggacat tgccagacag cactttgaac
    10261 gggtgaagga gcttacgatc aagctttcca tggtggaaaa ttctgcctat cgcctggcta
    10321 attccatcaa gaaaacccag aagcgcgcca atgccttaaa aaacatcaca attccccatt
    10381 atgaagcgct ttccagaacc atttccaatg cccttgagga gaaggaaaga gaggaattta
    10441 caagacttaa agtaataaag cggatgaagg ctgaaaaacc ttgacaaatc tgaacaaaac
    10501 agatataaat gataaggtaa aaactgacat tatcgaaaga agcggattcc tgaaggactg
    10561 tttaaaacgg gtctgcgccg ggcggcgctt tcatttcagg actcccgtca tctaggagga
    10621 aaaattgatg aataagacag agtttatcgc agcagttgct gagaaggcag agatttccaa
    10681 gaaggatgct gagaaggcag ttaaagcgtt cacagatgtt gtaactgagg agttagtaaa
    10741 cggcggcaag gtacagttag ttggatttgg taactttgag gttgctgaga gacctgcaag
    10801 agagggaaga aaccccaaga caggcgaggc tatgaccatc gcagcttcca agacaccaaa
    10861 gtttaaggcc ggcaaggcat taaaggatga ggttaataaa taattcatga gacttgacaa
    10921 gtttcttaag gtttcccgcc tgatcaagcg caggactgta gccaatgaag cctgcgacgc
    10981 aggacgcgtc ctggtcaatg ataaaccggc taaggcatcc ttaaatgtaa aaacaggcga
    11041 tattatcgag atccagttcg gagcaaaggc tgtaagagct gaggttctgg atgtacagga
    11101 aactgtaaaa aaggatgaag caaaggaact gttccgttat ctgtaggtca aaggtctgat
    11161 ggccggggtt cggaacaggc tgtggcatac aatggcccgc ctccccatat attaggttta
    11221 gggactgccc ttaaactgaa tatatgggga ggtttttgta tggaagaaac gcttggcagc
    11281 atccggcccc accggatcac cattcaggat cgcaagactg ccggaattac cggagtatca
    11341 gactgtgtgt cctttgatga aaatgaggtg gtgctggata ccgatatggg acttttgacg
    11401 attcggggaa aggagctcca tgtgaaccgt ctgactctgg aaaagggaga ggtagatctg
    11461 gacggaacgg tggaaagctt tgcctacagt tccaatgaag cattgcggcg gtcgggagaa
    11521 tccctgtttg cccgcctctt taagtgatag gaggtggggg cagatgagcg agcgtatccg
    11581 ctatgagctt cttcttacag gcttgagcct ggttatggga ggatggctta tgatcgtcta
    11641 tgacagctta aggctgctgc gccttttgat ccatcataat tcccttttta tgggaatgga
    11701 agattttttt tactggatct atgctggagg agccgtattt atgctgctgt atgaacagaa
    11761 tgacggaagc tttcggtttt atgccgtagc aggtgtattt ttaggtatgg cggcatatga
    11821 tcgttttgtg agccgtatct tcttccggtg cttgaaaaag tttagcagat ggtttaaaat
    11881 aacaagaaag ggtaattccg gatcgtagag agcgggagtc aggagaacag ggatggcata
    11941 ttatgggagg tcaaagcgtt taagacgcgg caaatgggaa aatcggatgg caatcatagg
    12001 tataactatg gtagtcattt ctcttgctgt ggctgtaaat gcaaaagggg cttctttaaa
    12061 gcaggcagac ctggattaca gacagaagga agcggatctg gcccgtcagg ttcaggatga
    12121 ggaaaacagg accagagagc ttgaggaata caaaatttat gtacagacca agcagtatgc
    12181 agaagaagta gcaaaggaaa agctgggtct ggtaaatccg gatgagatcc ttttaaagcc
    12241 cagacagtaa gtcgtgtgtc gaatggggtc gaaggatttt tgcacccttt aactgaatat
    12301 gacagatatt tcctctccgg tctgcgtata ctggataaga ggcgggcttt ttggtattgg
    12361 aagtccgtca gcgcagattg gagggatttt tttgttgtcg ggaagacggg aagtaagaag
    12421 cagatcagat gtaaattatt atacagccag gaaattggag gatatggcaa gatccatgga
    12481 gcggctggca aaagcttttg acgaggggat gcacaagaca ggaagcctga caagagatga
    12541 cgggctggct gctatgcaga cttccgcatc catggtgtgc caggactgca gccaatgcgg
    12601 tatttatgca gagagtgagc gggaggacag ttattatctg tattatcttc tccgggcatt
    12661 tgaacagaaa ggacagatcg aaaaggagga catgccccgc ccttttctgg ccggctgccg
    12721 caaaaaagag gattatctgg cccagttaaa ccgcagtctg gcccgtgctg ccatgaatct
    12781 gtcctggaag aaccgttttc tggaaagccg tgacgcagtg gtatcccagt ttcgcgaact
    12841 gtccctgatc ctgggagaat tttctcatca gatcgatcag gcggcagata taacggagga
    12901 atatggatac ataatgaaaa agctgttccg caggtgccat gtggcggtgg aaaatatgct
    12961 catcctggag tatgaaagcg gaagaagaga ggcttatgtt acagcccgca ccaccaacgg
    13021 acggtgcatg actgcaaagg atgcctcaga gcttatgagc gaggtgatcc ccgggacgag
    13081 gtggaaccct gcaaaggaca gccgcagcat tatcacaaga cagagcggaa ccgtgcgttt
    13141 tgaggaggat ggggagtatc agcttcttta cggggcagcc agagtgccga agcaggggga
    13201 acggtgttcc ggggataatt ataccttctg cgaaagtccg ggaagtcagg ccatgatcag
    13261 tctctgcgac ggcatgggct gcggtgagcc ggcctgtgag gaaagcggac aagtggtaga
    13321 actgacggaa aatcttttgg aagcaggttt tggatccaga gcagccttta agctggtaaa
    13381 taccgtgctt ttactggcag gaacggagca gcatccggcg gctctggaca tgagctgcgt
    13441 ggatctgtat acaggagtgc tggatgttat gaagctgggg gcagcgccta cctttgtgct
    13501 gggacaggag ggggcagaga ttttggaagc agggcaggtt ccggcaggga ttttaagtga
    13561 agcggagccg gtgatgctgt ccagaaagct gtgggatggg gatcacatta tcatggtgac
    13621 agacggggtg ttagatgccc ttccgggaga ggataaggag caggctatgt gccagtttct
    13681 ggaaagtctg gactttatgc cgcctcagga gatggcagaa cggattttgg aatttgccct
    13741 ttccttcgtg ccgggagcca gagatgatat gaccgtgctt acagcaggaa tctggaaaaa
    13801 ggagtagaaa gagaagacaa tggataagct gacagaacgg gtcagaaaga cgataaaaga
    13861 acatcatatg gtgaagccgg gagaccgggt gatggcggcg ctgtccggcg gcgcagattc
    13921 ggtatgcctg ctgcggatcc tggttcagtt gagagatatt ctgggaatcc ggctgagggc
    13981 cgttcatgtc catcatgggc tgcggggaga gga
//
LOCUS       DBMDUH010000060        13671 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_2402_length_13671_cov_6.465733, whole genome shotgun sequence.
ACCESSION   DBMDUH010000060 DBMDUH010000000
VERSION     DBMDUH010000060.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 13671)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13671)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13671
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2402_length_13671_cov_6.465733"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="ACZPCG_06725"
     CDS             <1..183
                     /locus_tag="ACZPCG_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284795.1"
                     /translation="KQADLLRELVTDLEHMNQVSEEVKNYYREKLDSIDQEMNVAEYG
                     CRELPYMDEAHREIIR"
     gene            235..507
                     /locus_tag="ACZPCG_06730"
     CDS             235..507
                     /locus_tag="ACZPCG_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284796.1"
                     /translation="MQKEKWKTMDVILVILAIFLLVFVIVMIAIYIRTGGIPDTLCTC
                     VFSVCGGECGVMGWIKTTKDRHLSRQYELEDRETDKKESMEETENE"
     gene            500..850
                     /locus_tag="ACZPCG_06735"
     CDS             500..850
                     /locus_tag="ACZPCG_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016294260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HIR1284797.1"
                     /translation="MSDICFEGLKILVMVAVLVLTRYVLPWVKSKADSEKLNLVAQWA
                     YKAVLMAQQTMKATDGKEKKAIVTQFLKELLQEKNIALSDTQIEILIEAAVKQMKIQE
                     NAGIVIEATDDVEV"
     gene            856..1629
                     /locus_tag="ACZPCG_06740"
     CDS             856..1629
                     /locus_tag="ACZPCG_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009297018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR1284798.1"
                     /translation="MGVRVGSARSNENGGVNGGKAGDQTGREVSTQLWYLHSKGWIVI
                     RAKAPAVREAIAKNMEAACQNDNIGYCQGHRGTATAAAKPYDYDLSKVNTAVETDCSE
                     LVRCCVLYAGIHVNSFSTANEVAALRQTGQFDILEKDEYCKASDYLLRGDILVTKTKG
                     HTVVVLDNGSKSSQNKKVEAAQKKDVSISGTYKTTADLNLRAGAGTTKDILVTIPKGA
                     AVSCYGYYSLYNGKPWYYVKTTVKGVAYTGFCSSAYLKR"
     gene            1709..2467
                     /locus_tag="ACZPCG_06745"
     CDS             1709..2467
                     /locus_tag="ACZPCG_06745"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - obsolete DNA methylation on
                     adenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HIR1284799.1"
                     /translation="MNSFIAWIGGKKLLRKEIVKRFPEEGFTRYVEVFGGAGWVLFEK
                     EQGKELEVFNDRDSNLINLYRCIKYHCGELQRELEWLTISREQFFDSKSQLDSQGLTD
                     IQRAARFFHIIKVSFGSDRRTFGTNKKNLANAIEYLPLIQKRLQGVVIENKDFENLIR
                     VYDRPGALFYLDPPYHGTEKYYEGSFTEADHERLKAVLSSIKGRFILSYNDDTYVREL
                     YKGYNIEEISRNNSLAGKTKTSQFKELIIRNYEK"
     gene            2539..2820
                     /locus_tag="ACZPCG_06750"
     CDS             2539..2820
                     /locus_tag="ACZPCG_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016287183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1284800.1"
                     /translation="MDIMIKIHLSRLLGEKRWTQKDLAEATGIRPSTINEWYHEFVTR
                     INLDHVDKICEVLECDISDLIEYVPNQHKITGKDLIVEEHGNRKPKKKQ"
     gene            complement(3076..4440)
                     /locus_tag="ACZPCG_06755"
     CDS             complement(3076..4440)
                     /locus_tag="ACZPCG_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005330778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1284801.1"
                     /translation="MNDNKDFLAKEPVGKLLLKLALPTVTAQIINMLYNIIDRIYIGH
                     IPENGALALTGVGVCMPLIMIVSAFAALVGNGGAPRASIFMGQSDKQAAEKTLGNCFT
                     VQLIISAVLTAVLLMWNREFLLAFGASENTVEYGVAYMNIYALGTIFVQLTLGMNAFI
                     TAQGFAKTGMLSVMIGAAANIILDPIFIFVFGMGVRGAALATILSQALSCTWVLAFLF
                     GKKTFLKIKASNLKLTRSIIMPSLALGLSTFIMQSSESVISVCFNSSLLKYGGDIAVG
                     AMTILTSVMQFAMLPLQGLGQGAQPIISYNYGAKLTGRVRSAYKLLLALSLSYASLLW
                     LGVMLAPQAFAAMFTSDPALIDFTKHALRIYMACMFLFGIQMACQMTFTAIGNAKASI
                     LVAVMRKFILLLPLIYLVPKFMPSDPTTAVYLAEPIADFFAVSFTAVLFFFQFKKALR
                     ALEE"
     gene            complement(4433..4885)
                     /locus_tag="ACZPCG_06760"
     CDS             complement(4433..4885)
                     /locus_tag="ACZPCG_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009265998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR1284802.1"
                     /translation="MTSNTDFLLGIRAAMKFQDSLLKQLSETYGLTLIEANVITFLHN
                     NPGKDTAADITELRMLSKGNVSQAVESLIQKGLLKRIPDLHDRRRIHLALLPASHSIT
                     ADVDTLMDEYRGYLFQGFSKEEFACYQILSKRLMENAKNAMKGRSTHE"
     gene            5042..6019
                     /gene="holA"
                     /locus_tag="ACZPCG_06765"
     CDS             5042..6019
                     /gene="holA"
                     /locus_tag="ACZPCG_06765"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008722703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HIR1284803.1"
                     /translation="MQILNQDIKDGSFKKAYLLFGEEAFLKMSYKNRLKDAVTAGDTM
                     NFNHYEGKGMDINEIIGIADTMPFFAERRLVLIEDSGWFKGGAGGEEMSAYMEHIPDT
                     TCLVFVETEVDKRSKLFKAVKKYGYTAELGRQDASQLGRWAAGILSKNGKKITGRTME
                     FFLSKTGDDMENISSELEKLISYTLGRDVITEEDVEAICTTQVSNKIFDMITAIANRQ
                     TRKAMDLYEDLLTLKEPPMRILFLIARQFNQILQVKELMGQGMDKSTIASRMKLQPFV
                     AGKIMLQAKTFSREQILSYVNLCVDAEESVKTGRLTDRLAVELLIAKTY"
     gene            6034..6597
                     /locus_tag="ACZPCG_06770"
     CDS             6034..6597
                     /locus_tag="ACZPCG_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016279973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HIR1284804.1"
                     /translation="MEELQLDAIRKQTKDVLRELMEKADLKQGQILVVGCSSSEIGSF
                     KIGSHSSGEIGQAVYEALYEELKPAGIYLAAQCCEHLNRALILEEEAALKYGYEPVNV
                     VPQLKAGGSFATAAYHTLEHPAAVEHIRAHAGIDIGDTLIGMHLKDVAVPVRILNREI
                     GGAHVVCARTRPKFIGGERARYDERKL"
     gene            complement(6654..6917)
                     /gene="rpsT"
                     /locus_tag="ACZPCG_06775"
     CDS             complement(6654..6917)
                     /gene="rpsT"
                     /locus_tag="ACZPCG_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008722714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HIR1284805.1"
                     /translation="MANIKSAKKRILVNETKAARNKAIRSKVKTAIKKVEAAVAAGDK
                     AAAQTALQAAITEIDKACTKGVYHKNNAARKVSRVTKAVNTLA"
     gene            7217..8209
                     /gene="gpr"
                     /locus_tag="ACZPCG_06780"
     CDS             7217..8209
                     /gene="gpr"
                     /locus_tag="ACZPCG_06780"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HIR1284806.1"
                     /translation="MGSSENKQAAATFSFQIRTDLALEDKESFEGDGGEIPGVVLKER
                     TRKEGAVRMTEVKILNAQGARAMGKPEGTYLTLEADELQTPDEDCHSRVVDELAMQLN
                     ELMKRAGWEKKENPSLLVVGLGNQSVTPDSLGPRTVGNLRVTGHLLADRTEKKQPYPC
                     LCAIAPGVMAQTGMETAQILHGIIGETSPDLVIAIDALAARSVRRLCTTIQLTDTGIH
                     PGSGVGNHRHSLTKESLGVPVIAIGVPTVVGAAAIVQDTISALLAVLEENECAKGTGS
                     WIRQMDTEGQYELIRELLEPEFGPMYVTPPDIDQSVQQLSFTISEGIHRAVFEL"
     gene            8305..9603
                     /locus_tag="ACZPCG_06785"
     CDS             8305..9603
                     /locus_tag="ACZPCG_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HIR1284807.1"
                     /translation="MGMKRNGRRGPVRICLMGLAAAGTVWGAVYMIKNSRAVLTAVPW
                     EQQVRMAGEELAALLWHQAAPYEMTGKNSRNITEQDGSNTDQAVKIAWEDADPVFRNH
                     MQARLFYREHQYLDRYGDETTGEKQHEERKKASQGSMAHGLEESPAESFGEALEETAA
                     SVSGSALSLAGSLPLPVTGTLYQLQQLEDFDFLMKHFYSVHTSTTAGRDLFNGPALLS
                     HDVTLDKEGEGPQILIYHTHSQEAFKDSGPQGRTIVEVGDYLAELLTQKGYRVYHDTS
                     VYDLQSGVLDRSKAYNYALEGVNRILQQNPSIQVVLDLHRDGVSENLHMVSDINGKPT
                     AQIMFFNGLSRTPEGKIAYLPNPYLAENLAFSLKMQLGAQAYFPGFARKIYLKGLRYN
                     LHLRPKSALIEAGAQTNTFEEVKNAMEPLAQLLDMVLQGE"
     gene            9658..11472
                     /gene="lepA"
                     /locus_tag="ACZPCG_06790"
     CDS             9658..11472
                     /gene="lepA"
                     /locus_tag="ACZPCG_06790"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HIR1284808.1"
                     /translation="MATIDQSKIRNFCIIAHIDHGKSTLADRIIEKTGLLTSREMQEQ
                     ILDNMDLERERGITIKSQAVRTVYKAKDGEEYIFNLIDTPGHVDFNYEVSRSLAACDG
                     AILVVDASQGIEAQTLANVYLALDHDLDVFPVINKIDLPSADPDHVEAEIEDVIGLEA
                     HDAPRISAKTGLNIEDVLEAIVHKIPSPKGDPDAPLQALIFDSIYDSYKGVIIFCRVM
                     EGTVKKGTRVRMMATGAEEEVVEVGYFGAGQFITCDELSAGMVGYITASIKNVRDTRV
                     GDTVTDAVRPCESPLPGYKKVTPMVYCGLYPADGAKYNDLRDALEKLQLNDASLFYEP
                     ETSVALGFGFRCGFLGLLHLEIIQERLEREYNLDLVTTAPGVVYRVYKTNGEMIELTN
                     PSNLPDPSEIEYMEEPIVSAEIMVTTEYVGSIMTLCQERRGVYLGMEYIETTRALLKY
                     ELPLNEIIYDFFDALKSRSRGYASFDYELKGYERSELVKLDILVNREEVDALSFIVHS
                     ASAYEKGRRMCEKLKEEIPRHLFEIPIQAAIGGKIIARETVKAVRKDVLAKCYGGDIS
                     RKRKLLEKQKEGKKRMRQIGNVEIPQKAFMSVLKLDEE"
     gene            11497..12693
                     /gene="hemW"
                     /locus_tag="ACZPCG_06795"
     CDS             11497..12693
                     /gene="hemW"
                     /locus_tag="ACZPCG_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HIR1284809.1"
                     /translation="MINANKNTIELYIHIPFCVKKCFYCDFLSFRALAADHEAYVDQL
                     VREIRARGAFCSELTVSTVFIGGGTPTVMEPELIRRIMEAVKENFTLEPDAEITIEAN
                     PGTLLYNKLPVYHDCGINRVSIGLQSADNQELKNLGRVHSFEEFLKSYQSARMSGFTN
                     ISVDLMSGIPGQTLESWKNTLKKVTMLKPEHISAYSLIIEQGTPFWEQYGPGMGGCRR
                     ENVLPLPDEDTENRIYHLTRDFLGEMGYQRYEISNYAKPGKECRHNIGYWTEVSYLGL
                     GLGASSYMDGCRFSNEKDLRRYLALDLEHDCGEGLRELAGPVEQLSREARMEEFMFLG
                     LRMTRGVSEMDFVRLFGVKMETVYGAVIERLMANGLLKKEGVWLSLTEWGMDVSNFVL
                     SEFLLS"
     gene            12710..>13671
                     /locus_tag="ACZPCG_06800"
     CDS             12710..>13671
                     /locus_tag="ACZPCG_06800"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HIR1284810.1"
                     /translation="MGKYIMALDAGTTSSRCILFDQNGEVCSLAQKEFTQYFPKPGWV
                     EHDANEIWSTQLGVAVEAMSKIGASAGDIAAIGITNQRETAIVWDKATGEPIGPAIVW
                     QCRRTAAYCDELKAKGLTELIRQKTGLVIDAYFSATKLKWILDHVPGARERAGRGELL
                     FGTVETWLIWKLTKGAVHVTDYSNASRTMLFNINTLTWDKEILELFDIPEQMLPKPKP
                     SSCIYGMTDRQFFGDPIPIGGAAGDQQAALFGQTCFEPGDAKNTYGTGCFLLMNTGDR
                     PVFSKSGLVTTIAWGLEGNVTYALEGSIFAAGAAIQWLRDELRL"
BASE COUNT         3875 a         2758 c         3762 g         3276 t
ORIGIN      
        1 aagcaggcag atctcctccg ggagctggtg acagatctgg aacatatgaa ccaggtttcc
       61 gaagaagtaa agaattatta tcgggagaag ctggattcta tcgatcagga aatgaacgta
      121 gctgaatacg gctgccgaga acttccctat atggatgaag ctcacagaga aataataagg
      181 taaaaacagg tgcatcggat atttccgatg tgccttttta attggaggaa gatcatgcaa
      241 aaagagaagt ggaaaaccat ggatgtgatc ctcgtaatcc tggcgatatt tttacttgtt
      301 tttgtgattg tgatgattgc tatttacata agaacaggag gaattccgga taccttatgt
      361 acctgcgtgt ttagtgtatg tggcggagaa tgcggagtaa tgggctggat caaaacaacg
      421 aaagacagac acctttccag acagtatgaa ctggaggata gggagacaga caaaaaagaa
      481 agcatggagg aaacggagaa tgagtgatat ttgctttgaa ggtttaaaga ttttggtgat
      541 ggtggcggtg ctggtattaa ccagatatgt acttccctgg gtaaaaagca aggctgattc
      601 cgagaagttg aacctggtgg ctcagtgggc gtataaagcg gttttaatgg cccagcagac
      661 catgaaagcc actgatggaa aagagaaaaa agccatcgtt acgcagtttt taaaagagct
      721 tcttcaggag aagaacatcg cactttcgga cacgcagatt gagatcctga ttgaagccgc
      781 agtaaaacag atgaagattc aggaaaatgc aggaattgtg attgaagcaa cggatgatgt
      841 ggaggtgtag tcaacatggg agtaagagtt ggaagcgcaa gaagcaatga aaatggtgga
      901 gtaaatggtg gcaaggcggg cgatcagacc ggcagagagg tgtcaacgca gctctggtat
      961 ctgcattcaa agggctggat cgttattagg gcgaaggcac ctgcagtccg ggaggcaatc
     1021 gctaaaaaca tggaagctgc ctgccagaat gacaatatcg gttattgcca gggccacaga
     1081 ggaacggcaa cggctgcagc gaagccatat gattacgatc tttccaaggt aaatacagcc
     1141 gtggaaacag actgctctga attggtacgt tgctgtgtgc tttatgctgg aattcatgtg
     1201 aatagtttca gcacagcgaa tgaggtggca gcactgaggc agactggcca gtttgatatc
     1261 ctggaaaaag atgagtattg caaagcttca gactatttgt tacgaggaga tatcctggtc
     1321 acaaagacga aaggccacac tgtggttgtt ctggacaatg gctccaagag cagccagaat
     1381 aaaaaagttg aagcagctca gaaaaaggat gtatctatca gtggaaccta taaaaccacg
     1441 gcagatctga atttgagagc gggagccggt acaacaaaag atattctggt tacaattccg
     1501 aagggagcag cggtctcctg ttatggatat tacagcctgt acaacggaaa gccatggtat
     1561 tatgtaaaga ctactgtgaa gggagtagct tacacgggct tctgtagcag tgcgtattta
     1621 aaacgttaga taaatgaagg ctctgggagt acgagctccc ggagcatcct gatttcatgc
     1681 cccttaaaat tgaaatcagg aggacataat gaatagtttt attgcatgga tcggtggaaa
     1741 gaaattactg agaaaagaga ttgtaaaaag gtttccagaa gaaggcttta cacggtatgt
     1801 ggaagttttt ggtggagctg gatgggttct ttttgaaaaa gagcagggaa aagaactgga
     1861 ggtattcaac gaccgcgaca gcaacttgat aaacttatat cggtgcatca agtatcattg
     1921 cggagaactg cagagagagc tggaatggct gactatatcg cgggaacagt tctttgacag
     1981 caagagccag cttgacagtc agggacttac ggatatccag agagcggcgc ggttctttca
     2041 tattataaag gtgagctttg gttcagatcg cagaaccttc ggaacgaata aaaagaacct
     2101 tgctaatgct atcgagtacc ttcccctcat tcaaaagagg cttcagggtg ttgtaatcga
     2161 gaacaaggac tttgaaaacc tgattcgggt ttatgataga ccaggggcac tcttttatct
     2221 tgatccgcct tatcatggga cggaaaaata ttatgaggga agcttcaccg aagcggatca
     2281 tgagcgttta aaagccgttt taagcagtat caaaggccgt tttatcttat cttataatga
     2341 tgatacttac gtcagagaac tgtataaggg ttacaatatc gaagaaataa gccggaataa
     2401 cagcctggcg ggtaagacga aaacttcgca attcaaggaa ttgataatca ggaattacga
     2461 aaaataacgt atttcgttat aatataacaa aatgactttt aaagagtgcc ctcgtgctag
     2521 aataaccagc aaaggggcat ggatatcatg attaaaattc atttatcacg gcttcttgga
     2581 gaaaaaaggt ggacgcagaa agatcttgca gaagccactg gaatccggcc aagtacaatc
     2641 aacgaatggt atcacgaatt cgtaacccgt ataaacctgg atcacgttga caagatttgt
     2701 gaagtattag agtgcgatat ttcagatcta attgaatatg taccgaatca gcacaaaatc
     2761 acgggaaaag atctgattgt tgaagagcat ggaaaccgaa agccgaagaa aaagcagtag
     2821 aaaaaggacg ttttggaggg tattttacag ccctttaaaa cgtccttttt ctgtttttaa
     2881 aataggtttt tgcaaatgac gcgaaaacat tttgcatttt gcgcgaaaag ctacaggcag
     2941 catactgcaa aatgcggaaa cccaggagaa attgaaagac gaagagagca ctgcaaaatc
     3001 atcagattca ggtgattgag cagtgctctc ttttataaaa ttcagataga gaaaatacca
     3061 aacattccgc agtgattact cttccaatgc tctgagcgcc ttcttaaact ggaagaagaa
     3121 cagcacagca gtgaagctga ctgcaaagaa atcagcgatc ggctctgcca gatatacggc
     3181 agtagtcgga tcggaaggca taaacttagg aaccagatag atcagaggca gcagcaggat
     3241 gaacttacgc ataacagcga ccaggatgga agcctttgca ttgccgatag cagtgaaggt
     3301 catctggcag gccatctgga tgccgaagag gaacatacag gccatataga tccgcagtgc
     3361 atgcttggta aagtcgatca gagccgggtc tgaggtgaac atggcggcaa atgcctgagg
     3421 agcaagcatg acgcccagcc acagcaggga tgcataggat aagctcaagg cgagaagcag
     3481 cttataggct gagcgtacgc gcccagtgag ctttgcgccg tagttgtagc tgatgatggg
     3541 ctgtgcgccc tggcccaggc cctgtaaggg cagcatggca aactgcatga cgctggttaa
     3601 gatggtcata gcgccgactg cgatgtcgcc gccgtacttt aacagggagg agttaaagca
     3661 tacggagatt acgctttcac tggactgcat gataaaggtg gacagtccca gcgcaaggct
     3721 tggcatgata atggaacggg taagctttaa attggaagct ttgatcttta agaaggtctt
     3781 ttttccgaag aggaaggcca gcacccaggt acaggataat gcctgtgata agatagttgc
     3841 cagagccgcg ccgcgtactc ccattccaaa tacaaaaatg aaaatcggat ccaggataat
     3901 atttgctgcc gcaccgatca taacggagag catgcctgtt tttgcaaagc cctgggctgt
     3961 gataaatgca ttcataccca gtgtgagctg tacgaaaata gttcccagag cgtagatatt
     4021 catataggct acgccgtatt ctacggtatt ttcactggcg ccgaaagcca gaagaaattc
     4081 acggttccac attaaaagaa cagcggtaag aacagcggag ataatcagct gaacggtaaa
     4141 gcagttgccc agtgtttttt cagccgcctg tttatcgctc tgtcccataa agatcgaggc
     4201 tctgggagcg ccgccgtttc caaccagagc agcgaatgcg gacacgatca taataagggg
     4261 catgcagaca cctactcctg taagggccag agcgccgttt tcaggaatgt gtcctatgta
     4321 aatgcggtct atgatgttgt aaagcatgtt gatgatctga gcagtaacag taggaagagc
     4381 cagctttaaa agaagctttc ccacaggctc ttttgccaga aaatctttgt tgtcattcat
     4441 gtgtactcct gcctttcata gcattttttg cgttttccat gagccgtttt gaaagaatct
     4501 ggtagcaggc aaactcttct ttggaaaatc cctgaaacag atatcctctg tattcatcca
     4561 taagcgtatc tacatcagca gtaatggagt gtgaggcggg cagcagagcc aggtggatcc
     4621 ggcgtctgtc atggagatcc gggattctct ttaagagccc tttctgtatc aggctttcca
     4681 cagcctggga gacatttcct tttgagagca tccgaagctc cgtgatgtcg gccgcagtat
     4741 ctttccccgg attattgtgg agaaatgtga tgacatttgc ctcgatcaga gtcagtccat
     4801 aggtttcaga gagctgtttt agcaggctgt cctgaaactt catggcagcc cggataccta
     4861 aaagaaaatc agtgtttgag gtcattggat cctcctttta cataataaaa ggttctaaat
     4921 aaaaaggttt taaaaagaac tgttcagaaa aataattata cgccctacag tctatttgtc
     4981 aagaggggga agacatggta taatagagca aaacgccagg gcagggagtg acagacagcg
     5041 tatgcagata ttaaaccagg atataaagga cggttcattt aaaaaggcct accttctgtt
     5101 tggtgaagag gcatttttga aaatgagcta taagaaccgt ttaaaggatg cagtgacagc
     5161 tggagatacc atgaacttta accattacga gggaaaggga atggatataa atgagattat
     5221 cggcattgca gataccatgc ctttttttgc agagcgcagg ctggtgctaa tagaggacag
     5281 cggctggttt aagggcggag caggaggaga ggaaatgagc gcttatatgg agcatattcc
     5341 cgataccacc tgtcttgtgt ttgtggaaac agaggtggat aagcgcagca agctttttaa
     5401 agccgtgaaa aaatacggtt atacagcgga gcttggccgt caggatgcgt cgcagcttgg
     5461 ccgttgggct gccggcatcc tgtcaaaaaa tgggaaaaag atcaccgggc gcaccatgga
     5521 gtttttttta agcaaaacag gggatgatat ggaaaatatc agctcagagc tggaaaagct
     5581 gatcagctat accctgggcc gtgatgtgat cactgaggag gatgtggagg ctatctgtac
     5641 cactcaggtg agcaacaaga tttttgacat gattacggct attgccaacc gtcagacgcg
     5701 aaaggctatg gatctttatg aggatctgct taccttaaag gagccgccta tgcggatttt
     5761 atttctgatc gcaaggcagt ttaaccagat cctgcaggtg aaggagctga tggggcaggg
     5821 catggataag agtaccattg cttccaggat gaagcttcag ccctttgtgg caggaaaaat
     5881 catgctgcag gcaaagacat tttcaaggga acagatcctt tcctatgtaa atctttgtgt
     5941 ggatgcagag gaaagcgtaa agacaggccg cctgacagac cgtctggcag tagaactttt
     6001 gattgcaaaa acgtattaaa ggaggaaaat ggaatggaag aacttcagtt agatgcgatt
     6061 agaaaacaga caaaagatgt tttgagggag cttatggaaa aggcagatct gaagcagggg
     6121 cagattctgg ttgtgggctg ttcctccagt gaaatcggga gctttaagat tggctctcat
     6181 tccagcggag aaatcggaca ggcggtttat gaagctcttt atgaggagct aaaacctgcg
     6241 ggcatttatc tggccgccca gtgctgtgag catttaaacc gggcgcttat attagaggag
     6301 gaggccgcgt taaaatacgg atatgagcct gtcaatgtgg ttcctcagct aaaagcagga
     6361 ggctcctttg ctacggcggc ttatcatacc ctggagcatc cggcggcagt ggagcatatc
     6421 cgagctcatg cgggaatcga tatcggagat accctgatcg ggatgcattt aaaggatgtg
     6481 gcagttccag tgcggatttt aaacagagaa attggaggcg cccatgtagt ttgtgccaga
     6541 acacgcccta agtttatcgg cggagaaaga gcacgttatg atgaaaggaa gctgtaagca
     6601 aaaaaatccc tccggtgggt taatccggag ggattttaaa tatctctata aaattaagcc
     6661 agagtgttaa cagccttggt cacgcgggac accttgcggg cagcattgtt cttgtgatat
     6721 acgcccttag tgcaggcctt gtcgatctct gtgatagcag cctgtaatgc agtctgagca
     6781 gcagccttat ctccagcagc aacagcagcc tctaccttct tgatcgcagt cttaaccttg
     6841 gatctgattg ccttgtttct tgcagcctta gtctcgttta ctaagattct cttctttgca
     6901 gatttaatgt tagccaatga aatacacctc cgttcggttt tctcttctta atcgttgtgt
     6961 tttccatcaa agcctggaca cggacagttc aatgtaaaca tactattaca ttttagcgaa
     7021 tgattttggg aatgtcaagc ggaatttggt gtttttttga agttttaggg aatatttttt
     7081 tgaaatgagt ggaaaataag atggatcgca gtttctcttt ttgcgccgta cccgcagctg
     7141 agggtggatg gcctttggaa atactgcaaa atgtggaaat tcaaatggat cgaagaaaaa
     7201 gacaggaggc acaggaatgg gaagttccga aaataagcag gctgcagcca cattttcctt
     7261 tcagatacgc acagatctgg ctctggaaga taaagaaagt tttgaaggtg atgggggtga
     7321 gatcccaggg gttgtattaa aggaaagaac cagaaaagaa ggtgcggtca gaatgacaga
     7381 agtgaaaata ctgaatgctc agggagccag agcgatggga aagccggaag gaacgtatct
     7441 aacgctggaa gcagatgagc tgcagacgcc ggatgaggat tgtcacagca gagtggtgga
     7501 tgaactggcc atgcagttga atgagctgat gaaacgggca ggatgggaga aaaaggaaaa
     7561 tccgtccctg ctggtggtcg gccttggaaa ccaatccgta accccggatt ctttaggacc
     7621 ccgaaccgtg ggaaatctca gagtgaccgg ccatcttctt gcggacagga cagagaagaa
     7681 acagccctat ccatgccttt gcgccattgc tccaggcgtt atggcccaga cggggatgga
     7741 aacggctcag attctgcatg ggatcatagg agagacatca cctgatctgg tgattgccat
     7801 tgacgcattg gcagcacgca gcgtccgccg cctgtgcaca accatacagc ttacagatac
     7861 gggaatccat ccgggctccg gagtaggcaa tcatcgtcac agtctcacaa aagagagcct
     7921 gggagtaccg gtgatcgcta ttggagtgcc gacggtggtg ggggcggctg ccattgtgca
     7981 ggatacgatt tcagcccttc ttgcggtttt ggaggaaaat gaatgcgcaa aaggcacagg
     8041 atcgtggata cgccagatgg atacagaggg gcagtatgaa ctgatccggg agcttctgga
     8101 gccggaattt gggcctatgt atgtgacgcc gccggatatt gaccagtctg ttcagcagct
     8161 aagttttacc atttcggaag gtattcaccg ggctgtgttt gagctgtgat tccggcggca
     8221 gatgctgtca tttttttctt tgttttggca taccatagga tacagcactt tggcaaggtg
     8281 taaaaaggac aggcgggacc gggtatgggg atgaaaagga acggaaggag agggccggtg
     8341 aggatatgtc tcatgggcct tgcggctgcg ggaaccgtat ggggagccgt ctatatgata
     8401 aaaaacagca gagccgtcct tacggcggtt ccctgggagc agcaggttcg gatggccggg
     8461 gaagaactgg cagcgcttct gtggcatcag gctgcgcctt atgagatgac ggggaaaaat
     8521 tccaggaaca taacggagca ggacggcagt aatacggatc aggctgtaaa gatcgcctgg
     8581 gaagatgcag accctgtttt caggaatcat atgcaggcac gtttatttta cagggagcac
     8641 cagtatcttg accggtacgg agatgaaaca acaggggaga agcagcatga agaaagaaaa
     8701 aaggcctcgc aggggagcat ggctcacggc ttagaggaaa gccccgcaga aagcttcggg
     8761 gaagcgttag aggaaaccgc agccagtgtt tcaggctctg ccctttccct ggcaggaagc
     8821 cttcctctgc cggtaacagg aacactttat cagcttcagc agctggagga ttttgacttt
     8881 ctcatgaagc atttttacag cgtacatacc tctaccacag caggccgcga tctgtttaac
     8941 ggtccggcac ttttaagcca tgatgtgaca ttagataaag aaggggaagg gccgcagatc
     9001 cttatttacc atacccattc tcaggaggcc tttaaggatt ccggccctca ggggcggacc
     9061 attgtggaag tgggagatta tctggcagaa cttttgacgc agaagggcta ccgggtttac
     9121 catgacacgt cggtttatga tctgcaaagt ggcgttttgg atcggagtaa ggcctataat
     9181 tatgcactgg aaggcgtaaa ccgtatcctg cagcagaatc cttccattca ggtagttctg
     9241 gatcttcaca gggatggcgt ttcggaaaat ctccacatgg tttccgacat caatggaaag
     9301 cccacggcgc agatcatgtt ttttaacggc ctcagccgta cgccggaggg aaaaattgcc
     9361 tatcttccca atccttatct ggcagaaaac cttgcattca gcctgaaaat gcagctggga
     9421 gcacaggcgt attttccagg atttgccaga aagatctatt taaagggact gcgctataat
     9481 ctccacctgc ggcctaagtc tgctcttatt gaagcggggg ctcagacgaa tacatttgaa
     9541 gaggtaaaaa acgccatgga gcccctggca caacttttgg atatggtgtt gcaaggggag
     9601 tgaaaaaaag gtataatggc agatatattt taatttgaaa gaagacgagg ataaagaatg
     9661 gcaacgattg accagagtaa aatacgaaat ttttgtatca tcgcccatat tgatcacggc
     9721 aagtccaccc tggctgaccg tattattgaa aaaacagggc ttttaacaag cagagagatg
     9781 caggagcaga tcctggacaa catggacctg gagcgggaga gaggcattac catcaaatct
     9841 caggcagtgc gtaccgtata taaggcaaag gacggggagg aatatatttt taacctgatc
     9901 gacactcccg gacatgtaga ctttaattat gaggtttccc gcagtctggc agcctgcgac
     9961 ggagccatcc tggtagtgga tgcgtcccag ggcattgagg ctcagacgct ggcaaatgtc
    10021 tatctggctc tggatcatga tctggacgta tttccggtta tcaataaaat cgatcttcca
    10081 agcgccgacc cggatcatgt ggaggcggag atagaggatg tgattggtct ggaggctcac
    10141 gatgcgcccc gtatttccgc gaaaacagga ctgaatatcg aagatgttct ggaggccatt
    10201 gtgcacaaga tcccgtctcc caagggagat cccgatgctc cccttcaggc gctgatcttt
    10261 gattctattt atgattctta caaaggtgta attattttct gccgtgtgat ggaaggcacg
    10321 gtaaagaagg gaacccgtgt gcggatgatg gccacggggg cagaggaaga ggtagtagag
    10381 gtcggatact tcggcgcagg ccagtttatt acctgcgacg agctcagcgc gggaatggta
    10441 ggctatatta ccgccagtat caaaaatgtc cgcgataccc gtgtgggcga tacggttaca
    10501 gatgccgtcc gtccctgtga aagccccctt ccgggctata aaaaggtaac gcctatggtt
    10561 tactgcggac tttatcctgc agacggtgcc aaatacaatg acttaagaga cgcccttgaa
    10621 aagcttcagc taaatgatgc ttcgcttttc tatgaaccgg agacctctgt ggctcttggc
    10681 tttggcttcc gctgcggttt tttaggcctg cttcatctgg aaatcattca ggagcgctta
    10741 gagagggaat ataacctgga tcttgtgaca acggctccgg gcgtagtata ccgggtatat
    10801 aagacgaatg gtgagatgat cgagcttacc aatccatcca atctgccgga tcccagtgaa
    10861 attgaataca tggaggagcc tattgtaagc gcggagatca tggtaacgac cgaatatgtg
    10921 ggctccatta tgactttgtg ccaggagcgc cggggcgtat atctgggaat ggaatacatt
    10981 gaaaccaccc gtgccctttt aaaatacgag cttcccttaa atgagatcat ctatgacttc
    11041 ttcgatgcct taaagtcgcg gtccagagga tatgcctcct ttgattatga gttaaagggc
    11101 tatgaacggt ctgagcttgt gaagctggat attctggtaa acagagagga agtggacgct
    11161 ctttccttta ttgtacattc tgcctcggcc tatgaaaagg gacgcaggat gtgcgaaaaa
    11221 ttaaaggagg agattccgcg ccacttgttt gaaatcccca ttcaggcagc catcggtgga
    11281 aagataattg ccagagagac ggtaaaggca gtccgtaagg acgtgcttgc aaagtgctac
    11341 ggcggcgata tttcacgaaa gagaaagctt cttgaaaagc agaaggaagg caaaaagcgg
    11401 atgcgccaga tcggaaatgt agagattccg cagaaggcgt ttatgagcgt tctgaagctg
    11461 gatgaagagt aagagaaaaa gcgagacagt caccctatga taaatgcaaa taaaaatacc
    11521 attgagctgt atatccatat cccgttttgc gtgaaaaagt gtttttactg cgattttctc
    11581 tcctttcggg ctctggctgc agaccatgag gcttatgtgg atcagctggt aagagaaatc
    11641 cgggccagag gagccttctg cagtgagctt acggtatcca cggtttttat cggcggcggt
    11701 acccctacgg ttatggaacc ggaactgatc cggcggatca tggaggccgt aaaggaaaac
    11761 tttacattgg agccggatgc ggagattacg atcgaggcca atccgggtac tcttttatac
    11821 aacaagcttc ctgtgtacca tgactgtggc atcaaccgcg taagcatcgg ccttcagtcg
    11881 gcagataatc aggagcttaa aaacctgggc cgggttcatt cttttgagga gtttttaaag
    11941 agctatcaga gcgcaagaat gtctggcttt accaatataa gcgtggatct tatgagcggg
    12001 atccctggcc agacgcttga atcgtggaaa aataccttaa aaaaggtaac gatgctcaaa
    12061 ccggagcata tttccgcata cagcctgatc atagagcagg gaacgccctt ctgggaacag
    12121 tatgggccgg gaatgggcgg ctgccggaga gaaaatgtcc ttcctcttcc ggatgaagat
    12181 acggaaaaca ggatttacca tcttacaagg gattttctgg gagagatggg ctatcagcgc
    12241 tatgagattt caaactatgc aaagcctgga aaggaatgcc gccacaatat aggctactgg
    12301 acagaggtgt cctatctggg actgggcctg ggggcatctt cttatatgga cggctgccgc
    12361 ttttccaatg aaaaggatct gaggcgttat ctggccctgg atctggagca tgactgtggg
    12421 gaaggtctga gagaactggc tgggccggtg gagcagcttt caagagaggc ccgtatggaa
    12481 gaatttatgt ttctgggcct tcggatgacg agaggggtat cggaaatgga tttcgtaaga
    12541 ctgttcggag tgaaaatgga aacggtctat ggagcggtga tcgaacggct tatggccaat
    12601 ggtctgctga aaaaagaagg agtatggctg tcacttacag aatggggcat ggatgtgagt
    12661 aatttcgtac tcagcgaatt tttgttgtct tagagaaggg agcatatgta tggggaaata
    12721 tatcatggct ctggatgcgg ggactaccag cagccgctgt attctgtttg atcagaacgg
    12781 ggaggtctgc agccttgctc agaaggaatt tacccagtat tttccaaaac ccggctgggt
    12841 ggagcatgac gccaatgaaa tctggtcgac tcagctggga gtggcagtgg aagctatgtc
    12901 aaagattggc gcctcggctg gcgatattgc tgctattggc atcaccaacc agagggagac
    12961 agccattgta tgggataagg ctaccggaga gcccattggt ccggcaatcg tgtggcagtg
    13021 ccgtcggact gcggcttatt gcgacgaatt aaaggcaaag ggactgacag aactgatacg
    13081 ccaaaagacc ggtctggtca ttgatgccta tttctctgct accaagctga aatggatcct
    13141 ggatcatgtg cccggagcca gagagagggc cgggcgggga gaactgcttt ttggaaccgt
    13201 ggaaacctgg cttatctgga agcttacaaa gggagccgtt catgtgaccg attactccaa
    13261 cgcttccagg accatgctgt ttaatatcaa tacgcttacc tgggataaag aaatcctgga
    13321 gctttttgac ataccggaac agatgctccc aaagccaaag ccctccagct gtatttacgg
    13381 catgacagac cggcagtttt ttggagatcc gattccgata ggaggcgcgg cgggagatca
    13441 gcaggccgct cttttcggcc agacctgttt tgagccgggc gatgcgaaaa atacatacgg
    13501 cacaggctgc tttctactta tgaatacagg agaccggccg gtattttcaa agagcggcct
    13561 tgtgacaacg atcgcgtggg ggctggaggg aaatgtaacc tatgcgttgg aggggtctat
    13621 ttttgctgct ggagcagcga tccagtggct gagggatgag ctgaggctga t
//
LOCUS       DBMDUH010000061        13512 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_2424_length_13512_cov_6.314891, whole genome shotgun sequence.
ACCESSION   DBMDUH010000061 DBMDUH010000000
VERSION     DBMDUH010000061.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 13512)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13512)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13512
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2424_length_13512_cov_6.314891"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="ACZPCG_06805"
     CDS             <1..639
                     /locus_tag="ACZPCG_06805"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HIR1284811.1"
                     /translation="IAWGLEGNVTYALEGSIFAAGAAIQWLRDELRLIGSAADSEYMA
                     EKVKDTNGCYVVPAFTGLGAPHWDAYARGAILGLTRGVNRYHIIRATLDSLCYQTNDV
                     LEAMKMDLGQEVKRLKADGGASANNYLLQTQADLTGVQVDRPACVETTAMGAAYLAGL
                     AVGFWKNKEEIARGWKADRSFFPKIEEEVRCRMLKGWQKAVRCSYGWAKDGI"
     gene            complement(645..950)
                     /locus_tag="ACZPCG_06810"
     CDS             complement(645..950)
                     /locus_tag="ACZPCG_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HIR1284812.1"
                     /translation="MNRNIYVYIAVMAAVTYLIRLLPLTLIRKEIKNTFLRSFLYYVP
                     YVTLSVMTFPAILYATRSAAGGAAALAVAVLLAWKGKSLFHVSLAACTAVFLLEFFI"
     gene            complement(951..1658)
                     /locus_tag="ACZPCG_06815"
     CDS             complement(951..1658)
                     /locus_tag="ACZPCG_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002600659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HIR1284813.1"
                     /translation="MNQQKRPFYSGLIHGIPIGLGYLAVSFTFGIMAVSSGLSVFQSV
                     LISLTNLTSAGQFAGLGIITAGSSYLEMAAVQLVINLRYCLMSCSLSQKLDHKMPLFH
                     RFLMAYGVTDEIFGVSVCHPGTLSPFYSYGLMSAAVPGWTLGTLLGALSGSLLPERLL
                     NALGVALYGMFLAIIVPPARENRILRAVVLLSMTASFVFTLIPVLNTISSGFKIILLT
                     FVIAGGAALICPVKEEA"
     gene            complement(1676..2683)
                     /locus_tag="ACZPCG_06820"
     CDS             complement(1676..2683)
                     /locus_tag="ACZPCG_06820"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007713965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HIR1284814.1"
                     /translation="MKRILLLGTGGTIACKRTSHGLMPLLSSEEILSFVPDSRTFCSI
                     DSIQVLNIDSTNIQPSHWLTIEKAIEEHYKDYDGFVITHGTDTMAYTAAALSYLIRNS
                     AKPIVITGAQKPIDMENTDARTNLADSLRFASHPNAHGVNIVFDGKVIAGTRGKKERT
                     KSYNAFSSINFPYIATIQEEHILFYLDDKELLKEPVQFFCHLNPNVGLLKLIPSMDAS
                     LLDYMAEHYDAVIIESFGVGGLPSYDGGDYHRAVGRWTSLGKTVIMTTQVTNEGSNMS
                     VYEVGKNIKQEFGLLEAYDMTLEAAVTKTMWILGQTKDPKEIRRLFYQTVNRDILWKS
                     V"
     gene            2932..3363
                     /locus_tag="ACZPCG_06825"
     CDS             2932..3363
                     /locus_tag="ACZPCG_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HIR1284815.1"
                     /translation="MTLFWIAAFIVLTAAEALTVSLTCIWFAGGALAGLLVQVLGGSV
                     KLQLALFAAVSFALLLLVRPLAAGILKPKETRTNTDGLIGRKVVVKETIDNVRGTGSV
                     SLAGETWLARSLHEDRILTPGTAAVIREIRGAKLFVEQENE"
     gene            3375..4325
                     /locus_tag="ACZPCG_06830"
     CDS             3375..4325
                     /locus_tag="ACZPCG_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008722981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HIR1284816.1"
                     /translation="MLGMIGGFMAFIIVAVVILVILSSCIRIVPQAQALVVERLGGYL
                     GTYGVGIHFLIPFIDRVAKKVNLKEQVEDFPPQPVITKDNVTMQIDTVVFFYITDPKM
                     YAYGVERPILAIENLTATTLRNIIGDLELDETLTSRETINAKMQESLDLATDPWGIKV
                     TRVELKNIIPPAAIQEAMEKQMKAERERREAILKAEGEKKSMILVAEGNKESAVLNAE
                     AEKEAAILRAEAEKEKKIKEAQGQAEAIRTVQKATAEGIRYIREAGADQTVLQLKSLE
                     AFAAAANGRATKIIIPSEIQGIAGLVKSIAEVAGDGAAEK"
     gene            4369..5766
                     /locus_tag="ACZPCG_06835"
     CDS             4369..5766
                     /locus_tag="ACZPCG_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003510738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1284817.1"
                     /translation="MSAEQKVQKTKANGITEGSIFGQLLLFFFPILFGTFFQQLYNTA
                     DAVVVGRFVGKQALAAVGGTTSTLINLMVGFFVGLSSGATVVISQYYGAKKADKVHWA
                     VHTSVAFSVIGGVLFMAVGLVGARWALTAMHTPEDVMDHAVTYIRIYFLGMVPNLLYN
                     MGAGILRAVGDSRRPLYFLIGSCFVNIILDVVLVAVLRMGVAGAALATISSQLFSVIL
                     VILCLTRTQDMYKVEWRKIRIDSRMLQRIIRIGIPAGMQSVMYNISNIIIQAGVNNLG
                     TDNVTAWATYGKVDGLYWMMINALGISVTTFVGQNYGARRMDRVRKGAGACMVIGVVL
                     TAIVSTALYFWGYLFIELFTSDPQVQLISQSLIRFMVPTFITYITIEILSGTLRGVGD
                     AWMPLIITGVGVCLVRVIWIIFALPHFNTILAAAFCYPMTWALTSAAFAVYYYFFSSL
                     KRVNLKKIFLRHQYK"
     gene            5824..6006
                     /locus_tag="ACZPCG_06840"
     CDS             5824..6006
                     /locus_tag="ACZPCG_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HIR1284818.1"
                     /translation="MTSGEVIFNNVEEIVHFVNQAEKIRWDVDVCLGSCIVDGKSLLG
                     VMSFGTGKKLKIVIHN"
     gene            6029..6862
                     /gene="nudC"
                     /locus_tag="ACZPCG_06845"
     CDS             6029..6862
                     /gene="nudC"
                     /locus_tag="ACZPCG_06845"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HIR1284819.1"
                     /translation="MIQEIGEGRFNPAYMRKNPQEDDILLHYEYNKVMLIKEGDRYRL
                     PAFRDLKGEEDAVESVSYLFSIDSKGYYLADTFQMPEFGGFVMEGLQIFRSFEPMEDG
                     FAGITGSQIYRWRESRRFCGCCGGKMAPGEKERSMVCSACGHTEYPKISPAVIVAIRN
                     GDRLLMSRYARGAYRHYALIAGYLEIGETFEDCVKREVMEEVGLKVKNIRYYKSQPWA
                     FSDTVMIGFVADLDGDDTIRLQEEELSEAGWFTREEIIEYPPYISVGHEMMKAFKDGK
                     I"
     gene            6862..7545
                     /locus_tag="ACZPCG_06850"
     CDS             6862..7545
                     /locus_tag="ACZPCG_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HIR1284820.1"
                     /translation="MKEKKYILFDLDGTLTDPKEGITKAVQYSLAHYGIEETNLDMLC
                     PFIGPPLRDSYINFYGFSQEKATEAISVYREYFCREGWAQNKVYPGIPELLGALKNAG
                     KKLYVATSKPEPFAVQILEHFGLAGFFDGIGGADLEETRVRKGDVISYVRERFGLLRD
                     ETGRDLAPDIVMVGDREHDIIGAGEQGLESIGVLYGYGSRRELEESEAARIAESVEAL
                     RAILLGEML"
     gene            7700..8203
                     /gene="ilvN"
                     /locus_tag="ACZPCG_06855"
     CDS             7700..8203
                     /gene="ilvN"
                     /locus_tag="ACZPCG_06855"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HIR1284821.1"
                     /translation="MDDRIVLSLLVDNTAGVLARVAGLFSRRGYNIESLTVGVTADPR
                     YSRMTVVSLGDQTVLEQIKNQLDKLEDVRDIKELKPDRSVYRELMMVKVRASAQDRQA
                     ISAISNIFRATIVDVGRDSLTVMLTGDQSKLDALINLLEDYEILELARTGLTGLERGS
                     EDIRYLP"
     gene            8232..9251
                     /gene="ilvC"
                     /locus_tag="ACZPCG_06860"
     CDS             8232..9251
                     /gene="ilvC"
                     /locus_tag="ACZPCG_06860"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HIR1284822.1"
                     /translation="MSKIYYQEDCNLSMLDGQTIAIIGYGSQGHAHALNLKESGCNVI
                     VGLYEGSRSWKRAQEQGFEVYTAAEAAKRADVIMILINDELQADMYKKDIEPNLKDGA
                     MLMFAHGFNIHFGCIKPPKNVDVTMIAPKAPGHTVRSEYQAGKGTPCLVAVEQDYTGK
                     ALDRALAYGLAIGGARAGLLETTFRTETETDLFGEQAVLCGGVCALMQAGFETLVEAG
                     YDPKNAYFECIHEMKLIVDLIYQSGFAGMRYSISNTAEYGDYITGPKIITEDTKKAMK
                     KILTDIQDGTFAKDFLLDMSPAGRQVHFKAMRKLASEHPSEKVGEEIRKLYSWNNEED
                     KLINN"
     gene            9396..9623
                     /locus_tag="ACZPCG_06865"
     CDS             9396..9623
                     /locus_tag="ACZPCG_06865"
                     /inference="COORDINATES: protein motif:HMM:NF023166.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HIR1284823.1"
                     /translation="MGAEYERIVNEAVIVQIPQKEQETGVKFWNAEEGGFDGITEETD
                     FYLNEAGAPVVVFAPYEIAPGAAGTVEFVIK"
     gene            complement(9674..10555)
                     /locus_tag="ACZPCG_06870"
     CDS             complement(9674..10555)
                     /locus_tag="ACZPCG_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1284824.1"
                     /translation="MEQNLSQYKIFYEVAKAGNISKAAKELYISQPAISKAISKLEDS
                     IGIPLFTRSSRGVQLTAEGQVLFEYAKEAFEALTRGEQELKRIQEFDIGHLRIGVSNT
                     LCKYVLLPYLKTFIDQYPHMRISIEGQSTAQTIAGLEQHKLDLGLIAEPASHRDLTFI
                     PVMDIQDTFVTTPSYLENLYLREGKDADIFETGNIMLLDQSNMTRIHVDKYLEEYSII
                     PHQVLEVTTMDLLIEFAKIGLGVACVIRELVQKELDNGTLVEVPLKNAIHRRTIGFAY
                     HSNNQAMALKTFLEFLY"
     gene            10701..11963
                     /gene="leuC"
                     /locus_tag="ACZPCG_06875"
     CDS             10701..11963
                     /gene="leuC"
                     /locus_tag="ACZPCG_06875"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HIR1284825.1"
                     /translation="MGMTMTQKILAAHAGLAEVKAGQLIEAELDLVLGNDITSPVAIN
                     EMKKMNRQTVFDKDKIALVMDHFIPNKDIKSAENCKCCREFACRHEISNYFDVGDMGI
                     EHALLPEKGLVVAGDAVIGADSHTCTYGALGAFSTGVGSTDMAAGMVTGKAWFKVPSA
                     IRFELVGKPSKWVSGKDVILHIIGMIGVDGALYRSMEFTGEGIKNLSMDDRFTICNMA
                     IEAGGKNGIFPVDETALQYMKEHSKREFTVYEADEDAEYDETYVIDLSRLKPTVSFPH
                     LPSNTRTIDQVGEVKIDQAVIGSCTNGRIDDIRAAAEILKGKKVAKGVRCIVIPATQA
                     IYLQAMREGLLEIFIEAGAVVSTPTCGPCLGGYMGILAAGERCISTTNRNFVGRMGHV
                     DSEVYLASPAVAAASAVAGKITAPEAVM"
     gene            12015..12500
                     /gene="leuD"
                     /locus_tag="ACZPCG_06880"
     CDS             12015..12500
                     /gene="leuD"
                     /locus_tag="ACZPCG_06880"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003526189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HIR1284826.1"
                     /translation="MKVCGHVFKYGDNVDTDVIIPARYLNATKGEELAKHCMEDIDRE
                     FVNKVQPGDIIVAEKNFGCGSSREHAPLAIKCAGVSCVIAETFARIFYRNSINIGLPI
                     IECPQAAKAISAGDKVEVDFDSGVITNITRGEVYKGQSFPPFMQDIIQAGGLVAYINA
                     K"
     gene            complement(12487..13380)
                     /locus_tag="ACZPCG_06885"
     CDS             complement(12487..13380)
                     /locus_tag="ACZPCG_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1284827.1"
                     /translation="MEFREIVTFLQAAKLQSFSKAARQLGYSQAAVTIQIKQLEQELG
                     VHLFDRIGKQTTLTHQGQVFYEYASAIMRDLAQAKDAVANPTELTGQLCLGTIESICS
                     SIFPDLLTEYHRLHPKVNVNIITDSPDRLLEQMNANVIDIVYFLDKRIYDPKWEKVME
                     QPEQILFAASADHPLSAKKGELSLDEILSYPFVLTEKNASYRFILDQYLASEYKRLHP
                     FLETGNTDFILKFLQQNQGISFLPEFTIRQAVKEGRLCILTARDFHIQIWRQIVYHRD
                     KWVTREMAEFLRLAKEMATLR"
BASE COUNT         3565 a         2846 c         3769 g         3332 t
ORIGIN      
        1 atcgcgtggg ggctggaggg aaatgtaacc tatgcgttgg aggggtctat ttttgctgct
       61 ggagcagcga tccagtggct gagggatgag ctgaggctga ttggatcagc ggcagattca
      121 gaatatatgg ccgaaaaggt aaaggatacc aatggctgtt atgtggtgcc ggcttttacg
      181 ggtctgggag caccgcattg ggatgcctat gccagaggcg ccattctggg ccttacccgc
      241 ggggtgaatc ggtatcatat tatccgcgcg actctggatt ccctctgtta ccagaccaat
      301 gatgtgctgg aagccatgaa gatggatctg ggacaggagg tgaaacggct gaaggcagac
      361 ggaggggcca gcgccaacaa ctatctgctg cagactcagg cagatttaac cggcgtccag
      421 gtagaccgcc cggcctgtgt ggaaacaacc gccatgggag ctgcctatct ggcaggtctt
      481 gcagtgggat tctggaaaaa taaggaagag atcgccagag gatggaaagc ggaccgcagc
      541 ttcttcccaa agattgaaga ggaagtccgg tgcaggatgc ttaagggctg gcagaaggcg
      601 gtacgctgtt cctatggctg ggcaaaagac gggatttaag aagttcagat aaaaaattcc
      661 agtaaaaata cagctgtgca ggctgcaagg gatacatgaa aaagactttt tcccttccag
      721 gccagcagga cggccacggc cagagctgcg gctccgcctg ccgcagagcg ggtggcatag
      781 aggatggccg gaaaggtcat gacggagagc gtcacatagg gaacataata taaaaaagaa
      841 cgaagaaagg tatttttgat ttcttttcgt atcagtgtga gcggcagcag tcggatcaga
      901 taggtcactg ctgccattac tgctatatag acataaatat tccgattcat ttaagcttcc
      961 tcctttacag ggcagatcag ggcggctcct ccggcgataa caaaggtcaa aaggatgatc
     1021 ttaaatccgg atgaaatggt atttaatacc gggatcaggg taaatacaaa gctggctgtc
     1081 atggagagca ggaccacagc ccgaaggatc ctgttttctc ttgccggagg tacgatgatc
     1141 gccagaaaca ttccatagag ggccacgccc agggcattta aaagccgttc cggaagcaga
     1201 ctgccggaga gcgcgcctaa aagcgttcct aaagtccagc ccggcacagc ggcgctcatc
     1261 agcccgtagc tgtaaaaggg gcttaaggtt ccaggatgac agactgacac gccgaaaatt
     1321 tcatccgtca ccccataggc catgagaaaa cgatggaata aaggcatctt atgatccagc
     1381 ttctgggaaa gggagcagga cataaggcag tagcgcaggt tgatcacaag ctgcaccgcg
     1441 gccatttcca gataagagga gccggcggtt atgatgccaa ggccggcaaa ctggccggcg
     1501 cttgtaagat tggtaaggga gataagcacg gactggaaaa cagagaggcc ggagcttacc
     1561 gccatgatgc cgaaggtaaa ggatacagcc agatagccga ggccgatggg gatgccgtgg
     1621 atcaatccgg aataaaacgg acgtttctgc tgattcatac tcgtgtttcc ctcccttata
     1681 ccgatttcca gaggatatcc cggttgacgg tctgataaaa caggcgccgg atttccttcg
     1741 gatcctttgt ttgtcccaga atccacatgg tctttgtcac tgctgcctcc agagtcatgt
     1801 cataggcttc cagcaggccg aattcctgct tgatattttt gcctacctca tatacggaca
     1861 tattgctgcc ctcatttgtg acctgtgtgg tcatgattac cgtttttccc aggctggtcc
     1921 agcgtcccac ggcccgatgg taatctcctc cgtcatagga gggaaggccg cctacgccga
     1981 aggattcaat gataacagcg tcatagtgtt ctgccatata gtcaagaagg gaggcgtcca
     2041 tggaaggaat cagctttaaa agccccacat tgggatttaa atggcaaaaa aactgcaccg
     2101 gctcttttaa aagctccttg tcatccagat aaaaaaggat atgctcctcc tggatcgtgg
     2161 caatataggg aaaattgata ctggaaaagg cgttgtagct cttggtgcgc tccttttttc
     2221 cgcgggtgcc tgcgatcacc ttgccgtcga atacgatatt gaccccatgt gcgtttgggt
     2281 gagaggcgaa gcgaaggctg tccgcaagat tggttctggc gtctgtgttt tccatatcga
     2341 taggcttttg ggctccggtg atcacaatgg gctttgcaga attgcggatc agataggaaa
     2401 gggcagcggc cgtgtaagcc atggtgtctg taccgtgggt gatcacaaag ccgtcataat
     2461 ctttgtaatg ttcctcaatg gccttttcaa tggtaagcca gtgggagggc tgtatgttag
     2521 tgctgtcaat atttaatacc tggatcgaat caatgctgca gaacgtccgg ctgtccggga
     2581 caaaggaaag gatttcttct gaggaaagaa ggggcattaa cccatgggac gtacgtttgc
     2641 aggcaatggt gcctccagtg ccaagaagca ggatgcgttt cataaaatat aaatctcctt
     2701 atcaataacc ggccggcctt taaagcccaa accggagagt ctgtctgtat tctaacgatt
     2761 ctcttacgat ttgtcaattt tttcattccc ttatttgaat atgacagtaa atatgataca
     2821 ataaagatat ctttcctcaa aagcgggtac agccagctat aatggggaat catatccgtg
     2881 cggagtgtac agacagcttt ccggcacggc tgcagaaagg aggaaaagca gatgacctta
     2941 ttttggattg cggcatttat cgtgcttaca gcggcagagg cgcttaccgt ttcccttacc
     3001 tgtatctggt ttgcaggggg agcgcttgcg gggcttttgg ttcaggttct gggaggcagc
     3061 gtgaagctgc agctggcttt gtttgcagcc gtttcctttg cgctgctcct tctggtgcgg
     3121 cctctggcgg caggaatcct aaagccgaag gagacacgga ccaacacaga cgggctcatt
     3181 ggcagaaaag tggtggttaa ggagaccatc gacaacgtcc ggggaacagg aagcgtatcc
     3241 ttagccggag aaacctggct ggcacgttcg ctccatgagg accggatcct tacgcccggt
     3301 acagctgctg tgatccggga gatccggggc gcgaaactat ttgtagaaca ggaaaatgaa
     3361 taaggaggga ttttatgtta ggtatgatag gaggttttat ggcgtttatt attgttgcag
     3421 ttgtaattct ggtgattctt tcatcctgta tccgcattgt tcctcaggca caggccctgg
     3481 tagtggaacg tctgggcggt tatctgggaa cctacggagt gggcatccat tttctgatcc
     3541 cctttattga ccgggtggca aagaaggtaa acttgaaaga gcaggtggag gacttcccgc
     3601 cgcagccggt gatcacaaag gacaatgtta ccatgcagat cgatacagtg gtattcttct
     3661 acattaccga tccgaaaatg tatgcatatg gtgtggagcg tccgatcctt gctattgaga
     3721 acctgacagc tactactttg agaaatatta tcggcgatct ggagttagat gaaacactga
     3781 cttccagaga aaccatcaat gccaagatgc aggaatccct ggatctggcg acggatccct
     3841 ggggtatcaa ggttacccgt gtggagctta agaacatcat tcccccggca gctattcagg
     3901 aggccatgga gaagcagatg aaggcagaac gtgagcgccg cgaggctatc ttaaaggcag
     3961 agggcgagaa gaagtccatg atcctggtag cagagggaaa taaggagtct gccgttttaa
     4021 atgcggaggc tgaaaaagag gcggccatcc ttcgggcaga agcagagaag gaaaagaaga
     4081 ttaaggaggc tcagggacag gcggaggcta tccgtaccgt acagaaggct acggcagaag
     4141 gaatccgcta catccgggaa gcgggagccg atcagaccgt gctgcagctt aagagtctgg
     4201 aggcctttgc ggcggctgcc aacggcagag ccaccaagat catcattcct tctgaaatcc
     4261 agggaattgc aggtcttgta aagtcaattg cagaggtagc aggagacgga gctgctgaga
     4321 agtaagcaga taaagagata aagtacccgt caacgaggag ataaacaaat gagtgcggag
     4381 caaaaagtcc aaaagacaaa agcaaatgga attacagagg gaagtatttt cggacagctg
     4441 ctgctgtttt tcttccctat tttattcgga accttttttc agcagcttta taatacggca
     4501 gatgctgtgg tagtaggccg ctttgtggga aaacaggctc tggcggcagt aggaggaacc
     4561 accagtacgc tgatcaatct gatggtgggc ttctttgtgg ggctgtccag cggcgctact
     4621 gtggtcatct cgcagtatta cggtgcaaaa aaggccgaca aggtccactg ggccgtacat
     4681 acctcagttg ctttttcggt cataggaggc gttcttttta tggcagtagg acttgtgggg
     4741 gccagatggg ctctcacagc catgcataca cctgaggacg taatggatca tgcagtaacc
     4801 tatatcagga tctattttct ggggatggtt cctaatcttc tgtacaatat gggagccggc
     4861 attctccgtg cagtgggaga ttccagacgg ccgctgtatt tcctcatagg gagctgtttt
     4921 gtaaatatta tcctggatgt ggttttggtg gcagtgctcc ggatgggagt ggccggtgct
     4981 gctctggcaa ccatttcttc ccagcttttt tcagtgatcc tggtaattct ctgcctgacc
     5041 cgcacacagg atatgtataa ggtggagtgg agaaaaatcc gtattgacag ccggatgctg
     5101 cagaggatca tacgcatcgg tattccggcg ggaatgcagt ctgtaatgta taacatttcc
     5161 aacattatta tccaggccgg tgtaaataat ctgggtacag acaatgtgac tgcctgggca
     5221 acctacggaa aggtggacgg cctgtattgg atgatgatca atgctctggg aatatccgtt
     5281 actacctttg ttggacagaa ctacggagca aggcggatgg accgggtgag aaagggagca
     5341 ggcgcctgta tggtgatcgg cgtggtgctt acggccattg tctcgacagc gctgtatttc
     5401 tggggatacc tgtttattga gctttttacc tcggatcctc aggtacagct catcagccag
     5461 agcctgatcc gttttatggt tcccaccttt attacctata tcaccattga gatcctttcc
     5521 ggtaccttaa gaggcgtggg tgatgcctgg atgcctctca ttattaccgg tgtaggcgtg
     5581 tgtctggtgc gtgtgatctg gattattttt gcactgccgc atttcaatac cattctggca
     5641 gctgcctttt gttatcctat gacctgggct ttaacctcag cagcctttgc ggtgtattat
     5701 tactttttca gcagcctaaa gagggtaaac ttaaagaaaa tttttttaag gcatcagtat
     5761 aaataggaaa atggcggaaa aacttcctgt atcagtaaaa aagcaaaagc aggaggttgg
     5821 cttatgacgt caggagaggt tattttcaat aatgtggagg aaattgtgca ctttgtgaat
     5881 caggcggaaa agatccgctg ggatgtggat gtctgtctgg gaagctgtat tgtggatgga
     5941 aaatcccttt tgggggttat gagcttcggt acaggcaaaa aacttaagat agtgatccac
     6001 aactaaagaa gaaagggaag cagtgccaat gatacaggaa atcggtgaag gcaggtttaa
     6061 tcctgcctat atgagaaaaa atccgcagga ggatgatatc ctgcttcatt atgaatacaa
     6121 taaggtaatg cttatcaagg agggggacag ataccgtctc cctgctttta gggatttaaa
     6181 gggagaggaa gatgctgtgg aaagcgtttc atacctcttt tctattgatt ccaaagggta
     6241 ttatctggca gatacgtttc aaatgccgga atttggcggc tttgttatgg agggactgca
     6301 gatcttccgc tcctttgaac ccatggagga cggctttgcg ggaattacgg ggagccagat
     6361 ttaccgttgg agagagagcc gcaggttttg cggctgctgc gggggaaaaa tggctccggg
     6421 agagaaagag cgttccatgg tatgctctgc gtgcggccat acagaatatc ccaagatcag
     6481 tccggcagtg atcgtggcga tccggaatgg ggatcggctg cttatgtcca ggtatgccag
     6541 gggagcttac cgtcattatg ctctgatcgc aggctatctg gaaatcggag agacctttga
     6601 ggactgtgtg aagcgggaag taatggaaga ggtgggactg aaagtcaaaa atatccgtta
     6661 ctataagagt cagccatggg ctttttccga tacggtgatg atcggctttg tggcagatct
     6721 ggacggagac gataccatac ggctccagga agaggaactg tcggaggccg gctggtttac
     6781 aagggaggag atcatagagt atccgcccta tatcagcgta ggccatgaga tgatgaaagc
     6841 atttaaggat ggaaaaatct aatgaaagaa aaaaagtaca tattatttga tctggatggc
     6901 actctgaccg atccgaagga aggaattaca aaggctgtcc agtattctct ggcacattat
     6961 ggcatagaag aaacaaatct ggatatgctc tgtcccttta taggtccgcc tttaagggat
     7021 agttacatca atttttacgg tttttcccag gaaaaggcca cagaggctat ttccgtgtat
     7081 cgtgagtatt tctgccggga gggctgggcg cagaataagg tgtatccggg aataccagag
     7141 cttttgggag cattgaaaaa tgcgggaaag aagctgtatg tggcgacctc caagccggag
     7201 ccctttgcag ttcagatctt agagcatttt gggctggctg gattttttga cggcatcggc
     7261 ggtgctgatc tggaggaaac ccgcgtgaga aaaggtgatg tgatttctta tgtaagagag
     7321 cgctttggtc ttttaaggga tgaaacaggc cgtgatctgg cgccagatat cgtgatggtg
     7381 ggagacaggg agcatgacat cataggagct ggggagcagg ggctggagag cattggcgtg
     7441 ctctatggtt acggaagccg cagggaactg gaagagagtg aggcggcacg gattgcagag
     7501 tcggtagaag cgctgagggc aattctcctt ggtgaaatgt tataacttct gttcataatt
     7561 gccattccta ttatacattt gacaaataaa ataatggttg ctatgataaa aactaatata
     7621 tggcacaggg aattttcaga caaatcctgc ttctggcagg gatgggaaat gaccggctga
     7681 tatggaggaa taagatatga tggatgacag aatcgtgttg tcgcttctgg tagacaatac
     7741 ggccggcgtt ctggcccgtg tagcaggcct gttcagccgc cggggctaca acattgagag
     7801 cctgaccgta ggcgtcactg cagatccgag atattccagg atgaccgttg tttctctggg
     7861 agaccagacg gttcttgagc agatcaagaa ccagctggac aagctggagg acgtaagaga
     7921 cattaaggaa ttaaagccgg atcgttccgt atacagagaa ctgatgatgg ttaaggtaag
     7981 ggcaagcgct caggatcgcc aggccatcag tgcgatttcc aatattttcc gtgcaaccat
     8041 cgtagatgtg ggaagggatt ccctgacggt catgctgaca ggagaccagt caaagctgga
     8101 tgcccttatc aatctgttag aggattacga gattctggag ctggcccgta ccggactgac
     8161 cggactggag cggggaagcg aagatatccg atacctgcca taaaatacaa cgtctcaagg
     8221 aggatattta tatgtcaaag atctattatc aggaagattg taatttatcc atgctggatg
     8281 gacagaccat tgccatcatc ggttacggca gccagggtca tgcccatgcg ctgaacttaa
     8341 aggaatccgg gtgcaatgtg atcgtaggcc tgtatgaggg aagccgttcc tggaagagag
     8401 cccaggagca gggctttgag gtttatacag cggcagaggc tgcaaaaagg gcagatgtga
     8461 tcatgatcct gatcaatgat gagcttcagg cggacatgta taagaaggac attgagccaa
     8521 acttaaagga cggcgctatg ctgatgtttg cccacggctt taatatccac tttggctgca
     8581 ttaagccgcc taagaatgta gacgttacca tgatcgctcc caaggctccg ggtcataccg
     8641 tgcgcagtga gtatcaggca ggaaagggaa ctccctgtct ggtggcagta gagcaggact
     8701 acacaggaaa agctttagac cgcgctcttg catacggcct tgccatcggc ggtgccagag
     8761 caggactttt agagactacc ttccgcacag agacagagac agaccttttc ggagaacagg
     8821 cagtcctctg cggcggcgta tgcgctctga tgcaggcagg ctttgaaact ctggtagagg
     8881 caggatatga tcccaagaac gcatattttg agtgtatcca tgagatgaag ctgatcgtag
     8941 atctgatcta tcagtccggc tttgcaggaa tgcgctattc catttccaat acggcagagt
     9001 atggcgacta catcactgga ccgaagatca ttacagaaga tacaaagaag gctatgaaga
     9061 agatcctcac agatatccag gacggaacct tcgcaaagga cttcctgctt gatatgtctc
     9121 ctgcaggccg tcaggtacac tttaaggcaa tgagaaaact ggcttccgag catccgtctg
     9181 agaaggtagg cgaagagatc agaaagcttt acagctggaa caatgaagaa gataagctga
     9241 tcaacaacta ataacgcatc aattagaaaa cggagcagat atcccaaaag agggaaccgt
     9301 caaacagacg gcctcttttg ggatattttg gaatttaaag tgttcacccg cctttgaagc
     9361 ctttctggca accggaggaa cagaggaaga atgtgttggg agcagagtat gagaggattg
     9421 taaatgaggc agtcattgtt cagatcccgc agaaagagca ggaaacagga gtgaagttct
     9481 ggaatgcaga ggaaggcggt tttgacggaa ttacagagga aactgatttt tatcttaatg
     9541 aagcgggagc ccccgtagtg gtgtttgcac cctatgagat tgcaccggga gcagcaggaa
     9601 ccgtggaatt tgtgatcaaa tagcccaaaa tacagctgtc ggaaaacgta catttctccg
     9661 gcagctgtta tgcttaataa agaaattcca gaaatgtttt taaagccatg gcctgattat
     9721 tggaatggta ggcaaagccg atggttcttc gatggatggc atttttcaga ggaacttcca
     9781 ctaaagttcc gttgtcaagc tccttttgta ccagttcccg tatgacacag gctacaccca
     9841 gaccgatctt tgcaaattcg atcagcagat ccatagttgt aacctccaga acctgatggg
     9901 gaataatgga atattcttcc agatatttat ccacatggat ccgggtcatg ttgctctgat
     9961 ccaaaagcat gatatttccc gtttcaaaga tatccgcatc ctttccttcc cgcagataga
    10021 gattttccag gtaagaaggc gtagtgacaa aggtatcctg aatatccata acgggaataa
    10081 aggtcagatc cctgtgagaa gccggttctg cgatcaggcc cagatccagc ttgtgctgtt
    10141 ccagaccggc gatggtctga gcagtagact ggccttcaat ggaaatcctc atgtgtgggt
    10201 actggtcaat aaaagtcttc agataaggaa ggagcacata tttacaaagg gtattgctga
    10261 cgccgatgcg gagatggccg atgtcaaact cctggatccg ctttaattcc tgttctcctc
    10321 tggtcagggc ctcaaaggct tcctttgcat attcaaaaag gacctgccct tctgctgtca
    10381 gctgcacgcc tctggagctg cgggtgaaaa gcgggatacc gatgctgtct tccagcttgc
    10441 tgatggcttt gctgatggca ggctggctga tatagagttc cttggcagct tttgaaatgt
    10501 ttcctgcttt ggctacctcg taaaagattt tatactgtga aaggttctgt tccatggtaa
    10561 acactcctgt ataacatttt ttcatataca ccattcatat tatgtatttt tattatagca
    10621 acagatatga tagaatgtaa agcacagatt aaaaacagat ggggctaaat gaaaagcccc
    10681 ctggtgagga ggatgaagca atgggaatga ccatgaccca gaagatatta gccgctcatg
    10741 cgggacttgc agaggtgaaa gcgggacagc tgatcgaagc ggagctggat ttggtgcttg
    10801 gaaatgacat cacctctccg gttgccatca atgagatgaa aaagatgaac cgtcagacgg
    10861 tttttgataa agataaaatt gcccttgtaa tggatcactt tattccgaac aaggatatta
    10921 aatcagcaga aaactgcaaa tgctgcagag agtttgcctg ccgccatgag atcagcaact
    10981 attttgatgt gggtgatatg ggaatcgaac acgcactttt gccggaaaaa ggtctggttg
    11041 tggctggtga tgcggtgatt ggagcggact cccatacctg cacctacgga gcgttgggag
    11101 ctttttctac gggagtgggg agcacggata tggcggcagg aatggtaacg ggaaaggcct
    11161 ggtttaaggt gccttctgcc atccgctttg agctggttgg aaagccctcc aaatgggtat
    11221 caggcaagga tgtgatctta catatcatcg gcatgatcgg tgtggacggt gccctgtacc
    11281 gctccatgga atttacggga gagggcatta agaatctgtc catggatgac cgctttacca
    11341 tctgcaatat ggccattgag gccggtggaa agaacggtat tttccctgtg gatgagactg
    11401 ctctccagta tatgaaggag cattccaaac gggaatttac ggtatatgag gcggatgagg
    11461 atgcggagta tgatgagacc tatgtgatcg atctatcccg gttaaagcca acggtatcat
    11521 ttccccacct tccctccaat accagaacca tcgatcaggt aggcgaggtg aagatcgatc
    11581 aggcagtaat cggctcctgt accaatggac ggattgatga tatccgggcg gcagctgaga
    11641 tcttaaaggg aaagaaggtt gcaaagggtg tgcgctgtat tgtgatccct gcgacgcagg
    11701 ccatctatct gcaggccatg agagaagggc tgcttgaaat atttattgag gctggggcag
    11761 tggtaagcac tcccacctgc ggcccctgcc tgggcggtta tatgggcatt ctggcggccg
    11821 gtgaaagatg tatttccact acaaaccgca atttcgttgg ccgtatgggc catgtggatt
    11881 cagaagtata tctggcaagc ccggcggtag ccgcagccag cgccgtggca ggaaagatta
    11941 cggctcccga agcagtgatg taaggctgcc gggacgatct ggttttacag tcctaaaaaa
    12001 gaagggagat taccatgaaa gtatgcggac atgtttttaa atacggcgat aatgtagata
    12061 cggatgtaat tattccggct cgttacttaa atgccacaaa gggcgaggag ctggcgaagc
    12121 actgtatgga ggatattgac cgtgaatttg taaataaggt gcagccgggg gatattattg
    12181 tggcagagaa gaactttggc tgcggatcat ccagggagca cgcacccctg gccatcaaat
    12241 gtgccggggt cagctgtgtg atcgcagaga cctttgccag gattttttac cgcaattcca
    12301 tcaatatcgg cctgcccatt atcgagtgtc ctcaggcggc aaaggccatc agtgcaggag
    12361 ataaggtaga ggtggatttt gacagtggcg tgatcaccaa tatcaccaga ggcgaggtct
    12421 ataaaggcca gtctttcccg ccctttatgc aggatatcat acaggcaggc ggactggttg
    12481 cgtatattaa cgcaaagtag ccatttcttt tgcaagcctt aaaaattccg ccatttcccg
    12541 ggtgacccat ttatcccggt gatagacgat ctggcgccag atctggatgt gaaagtccct
    12601 ggcagtaagg atacagaggc ggccttcctt taccgcctgc cggatggtaa attccgggag
    12661 aaaggagatt ccttgattct gctgtaaaaa tttaaggata aagtctgtgt ttcctgtttc
    12721 cagaaaggga tgcagccgtt tgtattcaga tgccagatac tggtccagta tgaaccggta
    12781 gctggcattt ttttctgtaa ggacaaaggg gtaggagaga atttcatcta aggaaagctc
    12841 tccttttttt gcacttaagg ggtggtctgc agaggcggca aaaaggatct gctccggctg
    12901 ctccatgacc ttttcccatt tgggatcgta gatccgttta tccagaaaat agacaatgtc
    12961 aattacattg gcattcatct gctctaacag gcggtccggg gagtctgtga taatatttac
    13021 gtttaccttc gggtgcagtc tgtggtattc ggtcagaaga tccgggaaaa tggagctgca
    13081 gatggattca atggttccaa ggcacagctg cccggtgagc tccgtgggat ttgccacagc
    13141 gtctttggcc tgagccagat cccgcatgat ggcagaggcg tattcataga aaacctgtcc
    13201 ctgatgggtc agggtggtct gctttccaat gcggtcaaag agatggactc ccagctcctg
    13261 ttccagctgc ttgatctgaa tagtgacagc tgcctgagag tatcccagct gcctggcggc
    13321 ttttgaaaaa ctctgaagtt tggctgcctg taaaaatgtg acgatttccc gaaattccat
    13381 accgattcct tctttaatta aaaatatttt aatcatatat ttaaaatgtt aaaatttaca
    13441 aaaatatatg gatatgatac agtaaaatta aagagaatgc aagaggttgg atttcccatt
    13501 tggcagtatg gc
//
LOCUS       DBMDUH010000062        13502 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_2425_length_13502_cov_6.206253, whole genome shotgun sequence.
ACCESSION   DBMDUH010000062 DBMDUH010000000
VERSION     DBMDUH010000062.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 13502)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13502)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13502
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2425_length_13502_cov_6.206253"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="ACZPCG_06890"
     CDS             complement(<1..464)
                     /locus_tag="ACZPCG_06890"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF015384.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HIR1284828.1"
                     /translation="MAMDLMVLMAVNQVLCYRAGAAQIVRGAALGAAWACVTAIFPQM
                     PLTFKAAGTYGAAGAAMAVAAFKVKGIKEISRAAAGIYLSAVILGGAMMVFEEQLRPG
                     GIFFPLACGFKVLGIPDLSGALMLLGGTASVFGAVCWLRGLTASLLQRKALC"
     gene            complement(686..2038)
                     /gene="ftsZ"
                     /locus_tag="ACZPCG_06895"
     CDS             complement(686..2038)
                     /gene="ftsZ"
                     /locus_tag="ACZPCG_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003503628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HIR1284829.1"
                     /translation="MLEIKINESENAARIIVVGVGGAGNNAVNRMIDENIAGVEFIGI
                     NTDKQALQFCKAPTAMQIGEKLTKGLGAGARPDVGEKAAEESSEEISQALKGADMVFV
                     TCGMGGGTGTGAAPVVAKIAKDMGILTVGVVTKPFRFEAKARMTNALNGIECLKQAVD
                     TLIVIPNDRLLEIVDRRTTMPDALKKADEVLQQAVQGITDLINVPGLINLDFADIQTV
                     MIDKGIAHIGIGKAKGDDKAIEAVKQAVSSPLLETTIEGASHVIINISGDISLIEAND
                     AASYVQELAGDDANIIFGAMYDENAQDEASITVIATGLDEHNSTSSVTKAMAGFTNFK
                     PKAPAAASAAQTERAAAPEAAATASAGFTRPAQPSFNRSFGQQSAGNTAPSFTAPRFG
                     QGTGSFSSSAAATAPASTAASSNGSNTAAPAQNQAPSFRPTANKEMQINIPDFLKNKR
                     "
     gene            complement(2204..2935)
                     /locus_tag="ACZPCG_06900"
     CDS             complement(2204..2935)
                     /locus_tag="ACZPCG_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HIR1284830.1"
                     /translation="MRKTNEKQHMILGIAVAVFLLTAILVLSVRIQTITVSGSSRYSA
                     EQAKELLFSGRMGKNSVIAYLRDRLNPHKQIPFVEDYKIVFHGPFHAEVIIYDKSIVG
                     YVSYMSSYMYFDREGIVVESSGRKLEGVPWITGMDLGRVVLYQPLPVADSRIFEEILN
                     LTQQLSLYEILVDQIRFDAYNQPILTIGQMEVILGNMGNIDSKLSVLNDILTDQPQLR
                     EIRGTLKLGDYSETNDRAGITFKKK"
     gene            complement(2944..4251)
                     /gene="murA"
                     /locus_tag="ACZPCG_06905"
     CDS             complement(2944..4251)
                     /gene="murA"
                     /locus_tag="ACZPCG_06905"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIR1284831.1"
                     /translation="MSAIRVSGCIPLKGEISIQGSKNAALPMMAAALLHKGLTVLTNV
                     PRIQDTACMMKILEALGCSCIRRGCSVFIDAGEIKKTQVPQEYVRSMRSSIILLGALL
                     GRMGQGNCCYPGGCLIGARPIDLHLMALNKMGAVIREEDGIIEACAGKDGRLKGTEIV
                     FSYPSVGATEQALLAAVLAEGVTIIRGAAMEPEISQLCGFLNNMGAVICGAGTKNLMI
                     QGVRSLRDSAFRVEGDRIVAGTYGAAVMAAGGDVILKGVRPSALKLPLALFSRSGGKV
                     TVSESSGRLQATGIGCEKEGRIRISSKGPAGPLALKTGPYPEFPTDLQSPFMAFLAAG
                     RGVSRIEERVFEGRFGIAGELEKMGALIRLEGKTAVIYGAGSLKGSAVKAADLRGGAA
                     LAVAALAAKGETVIEDCRHIERGYEDICRDLSALGARIQWIGE"
     gene            complement(4339..5562)
                     /locus_tag="ACZPCG_06910"
     CDS             complement(4339..5562)
                     /locus_tag="ACZPCG_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007713363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIR1284832.1"
                     /translation="MQQGSRTQAARAAYEYGNQKQAYEAGAVPKKKRERSFYDLSLVV
                     IIIVLTVFGLVMIYSASSYTAQLNDDLNNNSAYFMQRQGGIALGGFIFMLIISKMDYH
                     WLYRLAVPSYILSHILMVIVTLVGREVNGKKRWLDIGPIGFQPSEFAKIALIIMLAAV
                     ITKLGGKINQWAVMGKVFLLAVAIGAIVAANNLSTGIILVGIGFVMLFVACKVKWPFF
                     LAGGGALGILMTAGPLGKFLLNIHVLKPYQYNRIEAWLNPELDPEGKSFQILQGLYAI
                     GSGGLMGQGLGESIQKLGFLPESQNDTIFAIICEELGLFGAIAVIMLFLLMIYRFVMI
                     ANDAPDLFGALLVVGVMAHIAIQVILNIAVVTNTIPNTGITLPFISYGGTSVLFLMVE
                     MGIVLSVSNQIRAAV"
     gene            complement(5855..7213)
                     /gene="murD"
                     /locus_tag="ACZPCG_06915"
     CDS             complement(5855..7213)
                     /gene="murD"
                     /locus_tag="ACZPCG_06915"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HIR1284833.1"
                     /translation="MDQSQRVLVAGTGKSGIAAAKLILNRGGEVVLYDGNTALDEKEI
                     KARFEEGAKVNVVLGEIKRSDLLGVELCIISPGIPLTSPFVAVVDEARIPILGEVELA
                     YQCSAGKLAAITGTNGKTTTTALTGAMVRKKYEETFVVGNIGEPFTAKAAEMTEDSVA
                     VAEISSFQLETIMDFKPNVSVILNITPDHLDRHGTMETYTQIKECITMNQTEEDTVVL
                     NFDDPILKEFGKKEGLKPKVIWFSSKESLKDGFFLKGEEIIYAKAGKETVLLNVKELQ
                     LLGQHNYENVMAAAAAAMTLGVSLEAIGEAAREFKPVEHRIEFVRERTGVRYYNDSKG
                     TNPEAAIRALKAMPGPVLLIAGGYDKHSDYSDWAKEFSGRVKYLVLIGQTRDRIAACA
                     REHGFTDVMYAEDLQEAVQVCAVYADSGDCVLLSPACASWGMFKDFEERGRVFKECVM
                     AL"
     gene            complement(7255..8223)
                     /gene="mraY"
                     /locus_tag="ACZPCG_06920"
     CDS             complement(7255..8223)
                     /gene="mraY"
                     /locus_tag="ACZPCG_06920"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008724821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HIR1284834.1"
                     /translation="MIHETILAIIIAFAISALLCPIIIPFLHKLKFGQQVRDDGPQSH
                     LKKQGTPTMGGLIILSSIIITSLFYIPSYPKIIPVLFMTAGFGIIGFLDDYIKIILKR
                     SEGLTPLQKLAGQIVITGIFAWYVLNSGEVGTDMLIPFTGGFEDGKFLSLGILFVPAL
                     FFVTLGTDNGVNFTDGLDGLCTSVTILVATFLTIVAIGEDMGISPITGAVVGSLLGFL
                     LFNVYPAKVFMGDTGSLALGGFVASAAYMMRLPLFIPIIGLIYLVEVLSVIIQVTYFK
                     KTGGKRIFKMAPIHHHFELCGWSETRVVAVFSIVTAILCMVAYLGL"
     gene            complement(8302..10065)
                     /locus_tag="ACZPCG_06925"
     CDS             complement(8302..10065)
                     /locus_tag="ACZPCG_06925"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007713376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HIR1284835.1"
                     /translation="MNPNLTKHRKNIAVLGLLIAVAMTGLLGRLGFLMLVRADHYGEM
                     ARDLHERERTIKAARGRILDAGGNVIADNRTVCTISVIYNQVTDREEVIRSLCSILEL
                     SEEPVRKKVEKRSSREIIKTNVDKETGDKIRALKLDGVKVDEDYKRYYPMGSLASKVL
                     GFTGGDNQGIIGLEVMYEKYLKGKNGVILTLTDARGVELDKAAEDRIEPVAGNDLQIS
                     LDVNIQLYAQQLAYQVMEQKNAKKVSVIVMDPRNGELLAMVNAPEFDLNDPFTPDQNL
                     KSQSLKEEAAARSQTMGLQDRLNAMWRNTCINDTYEPGSTFKIITAAAGLEAGAVTLE
                     DTFSCPGFRVVEDRRIRCHKAGGHGTETFLQGAMNSCNPVFIDVGQRLGVDRYYKYFT
                     QFGLKGKTGIDLPGEAATIMHKKEKMGAVELATVSFGQSFQITPIQLITTAASIVNGG
                     RRVTPHFGVRVIRSDGGAVKELSYPQGKQILSSETSASMRYVLEQVVAEGSGKRAALE
                     GFRIGGKTATSEKLPRSLKKYISSFLGFAPADDPRVMALITIDEPQGIYYGGTIAAPV
                     IGELFKNILPYMGIRPEPKAG"
     gene            complement(10129..12462)
                     /locus_tag="ACZPCG_06930"
     CDS             complement(10129..12462)
                     /locus_tag="ACZPCG_06930"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002603393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HIR1284836.1"
                     /translation="MKTSRNTRPSPYGDRSEGRKRGSKSGHSKRVFTEHMRGKLAITA
                     IVITLALFALIWVLYGIIKENNDQYSKIVLSQRQQEFSSRILSYRRGDIVDRNGTLLA
                     TSEKVYNLILDPQQIMKKPEYYLEPTIQALVDCFGFDSGEIRSLIQEKSDRAYVPYKK
                     GKQLSYDQKVAFETMRKERNAAYYASEDESQSRLRIMGVWFEDQYRRVYPYGSAACSV
                     IGFSSADGTVGTGGIEQFYNEDLMGVNGREYGYLDEENNLEGVLKEPTNGKTVVSTID
                     LNIQNILQKYIDEWQTTVGSHVTAAIAMNPKNGEILGMATSNKFDLNDPRKIEGYSDD
                     QLYELGKKDAVSVYKREHPDTTLTQEQMIADTKREDVISYGRQVVWNQIWRNFCVSDT
                     YEPGSPSKILTVASGLEEGVLKGNEYFDCEGLLNVGGWPIKCTAYVKGGHGSLSLQES
                     IMQSCNVAMMRIGAMMGKDIFTKYQAIFGMGQKTGVDLPGEADGAGLIYSAENMGPTE
                     LATNAFGQNYNCTMIQMAAALCSVINGGSYYEPHVMRQIMNEQGSVVETKDPVLVRET
                     VSSSTSEFLKEAMFQTVENGTGGAAKVAGYHVGGKTGTAEKQPRSAKNYLLSFAGFAP
                     AEDPQLFAYVVVDVPNYPPGPQQAHSSFASGIFAKIMAEALPYMNVFPEAGAEEEQAG
                     DTAADEGINEPSGSEGEETEPETETRETEKVYETDETIGDGYESGLPDGVPADPNAPS
                     LSLEETSDGWKEKKESQSEEKEDLEGTAGETKASSEA"
     gene            complement(12440..12961)
                     /locus_tag="ACZPCG_06935"
     CDS             complement(12440..12961)
                     /locus_tag="ACZPCG_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HIR1284837.1"
                     /translation="MAQRMGRPTGRSGAYNRRNTRDMAYIGGTAAPKLEPEYRPERKA
                     PVRRKKTGLHQNQQLRRNRERAMAMDLPYVFLLAAACICTLYICVSYLHLQSGITARM
                     HNIEHLEAQLEKMKTENDALETSINSSIDLNKIYEIATKELGMVYARRDQVLLYDKTE
                     SEYVRQYEDIPEH"
     gene            complement(13008..>13502)
                     /gene="rsmH"
                     /locus_tag="ACZPCG_06940"
     CDS             complement(13008..>13502)
                     /gene="rsmH"
                     /locus_tag="ACZPCG_06940"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HIR1284838.1"
                     /translation="FRIIRDYGEDRFAKNIAKHIVKARQEKPVATTGELCEIIKAAIP
                     AKVRATGGHPAKRTFQAIRIELNHELDVLEESIDGMIRLLNSGGRLSIITFHSLEDRI
                     VKSRFRINEDPCICPPDFPVCVCGRKSMGRVITRKPILPSEEELEENSRSKSAKLRVF
                     ERGE"
BASE COUNT         3176 a         3882 c         2865 g         3579 t
ORIGIN      
        1 ctgcacaggg cctttctttg cagcagggac gctgtaagtc ctctaagcca gcagaccgcc
       61 ccaaaaacgg atgctgtgcc tccaaggagc ataagcgctc cggacagatc cggtatcccc
      121 agtaccttaa aaccgcaggc caatggaaaa aagatccctc cgggccttag ctgctcttca
      181 aaaaccatca tggctcctcc aagaatgaca gcgctcagat agattcccgc tgccgcccgg
      241 ctgatttctt ttatcccctt tactttgaat gcggctactg ccatggcagc gcctgctgcc
      301 ccatacgttc ctgccgcctt aaacgtcagc ggcatctgcg gaaatatagc tgttacacag
      361 gcccaggccg cccccaatgc tgcacccctc acgatctgcg cggctcctgc ccggtaacaa
      421 agcacttgat tcactgccat aagcaccatc aaatccatag ccatatttac agcaaatact
      481 tcatctatgt agactgtata aaccacctcg ctttggcctc acctccccta tcaggctgtc
      541 ctatggacat tataacgttt ctcatatgca aatactgtcg aagggagggg gaaaacagga
      601 aaaatttttc gacaaaaaaa ccggtgaaac ggataatgat atacatttat ccatttcacc
      661 ggtcaaatcg gttcattaaa attgtttatc tcttattttt cagaaaatcc ggaatattga
      721 tctgcatttc tttatttgca gtcggccgga aggatggcgc ctgattctgg gccggagctg
      781 ctgtattgct tccgttgctg ctggctgcag ttgatgccgg agcggtagct gcagcggaag
      841 agctgaagct tcctgttccc tgtccaaatc ggggagcagt aaaggaagga gcggtgtttc
      901 cagcactctg ctgtccaaag ctgcggttaa aggagggctg agccggtctt gtaaagcctg
      961 cggaagcggt agcggcagct tccggagctg cggcacgttc tgtctgggct gcagaagctg
     1021 cagcaggtgc cttgggctta aaatttgtaa agcctgccat tgcctttgtc acagaagaag
     1081 tggaattgtg ctcatccaga ccggtagcaa ttaccgtaat gcttgcctca tcctgagcat
     1141 tttcatcata catggcacca aagatgatat ttgcgtcatc tcctgccagc tcctgcacat
     1201 agcttgcggc gtcatttgcc tcgatcaggc tgatgtcacc ggaaatattg atgatcacat
     1261 ggcttgcacc ctcaatagtc gtctcaagca gcgggctgga aacggcctgc tttacagcct
     1321 caatggcctt gtcgtcgccc tttgccttgc cgataccgat gtgtgcgatg cccttgtcga
     1381 tcataacggt ctgaatatct gcaaagtcca gattgataag gcccggaaca ttgatcaggt
     1441 ctgtaattcc ctgaactgcc tgctgaagca cctcatctgc cttcttaaga gcatccggca
     1501 tggtggtccg gcggtcaacg atctccaaaa gacggtcatt ggggataacg atcagagtat
     1561 ccaccgcctg ctttaaacat tcgatcccgt ttaaagcgtt tgtcatacgg gcctttgcct
     1621 caaaacggaa aggctttgtc acaacgccta cggtcaggat acccatatcc ttggcaatct
     1681 tcgcaacaac aggagctgct ccggttccgg ttccgccgcc catgccgcag gtaacgaata
     1741 ccatatccgc accttttaat gcctgtgaga tctcctcaga actttcctct gcggccttct
     1801 cacctacatc cggtcttgcg cctgcaccca gtccctttgt cagcttttca ccgatctgca
     1861 tggctgtggg agccttgcag aactgaaggg cctgcttatc cgtattgatg ccgatgaact
     1921 ccacacctgc gatgttctcg tcgatcatgc ggtttactgc gttgttgcct gcgccgccca
     1981 cacctactac aatgatcctt gcggcatttt ctgactcatt tatcttaatc tctaacaaga
     2041 tacttatcct ccttatccct ttcatgcatc ccggctctca gagtctgttt tcagaatgcg
     2101 tctctgttct cttttcttca gatattcttt aatatgtcat gcttttagcc taactaatac
     2161 tatatttgat tttattaaaa aaatcaaccc tgaattttgt attttatttc tttttaaatg
     2221 tgatccctgc ccggtcattt gtctcgctgt aatcccccag tttcagtgtt ccacggattt
     2281 ccctcagctg gggctgatct gtaagaatat catttaaaac agaaagcttg ctatcaatat
     2341 ttcccatatt tcccaagatc acttccatct gtccgatggt caggatcggc tggttatacg
     2401 catcaaagcg gatctgatct accaggatct cataaaggga aagctgctgg gtcaggttca
     2461 agatttcctc aaagatccgg ctgtctgcaa ccggaagagg ctgatagagc accacccgcc
     2521 ccagatccat gcctgtgatc cagggtaccc cctctaactt tctgccggaa ctttccacta
     2581 caatgccttc ccggtcaaag tacatatagc tgctcatgta ggaaacatag cctacaatgc
     2641 ttttatcata aataatgacc tctgcatgaa aagggccgtg aaacacgatt ttataatcct
     2701 ctacaaatgg aatctgctta tggggattaa gtctgtcccg cagataggca atgactgaat
     2761 tttttcccat ccggccggaa aagagcagtt cttttgcctg ctctgcactg tagcggctgc
     2821 ttcctgatac cgtgatcgtc tggatacgga cagacagcac aaggatagcc gtgagcagaa
     2881 aaaccgccac ggcaatcccc aggatcatat gctgtttttc gtttgttttt ctcatgcggt
     2941 atatcactct cctatccatt ggatcctggc tccgagagcc gataaatccc gacagatgtc
     3001 ctcataccca cgttcaatat gacggcagtc ctcgatgacc gtctctccct ttgcagccag
     3061 cgccgctact gccagcgctg cccctccccg aagatctgct gcctttacgg cagagccctt
     3121 cagggaacct gctccgtaaa taactgccgt tttcccctcc agccgaatta aggcccccat
     3181 tttttcaagc tctccagcta tgccgaaccg cccttcaaac acacgctctt caatccggct
     3241 cacgccccgg cctgcagcca gaaaagccat gaacggagac tgcagatccg tagggaattc
     3301 cggatacggc cccgttttaa gagcgagagg cccggcagga cctttgctgc tgatccggat
     3361 ccttccctct ttttcacagc cgatccctgt cgcctgaagc cgtccgctgc tttcagaaac
     3421 tgtaaccttt cctccggatc gggaaaaaag ggccagagga agcttgagcg ccgacgggcg
     3481 gacgcctttt aagattacgt ctcctccagc tgccatcaca gcagccccat aggttcccgc
     3541 cacgatccgg tcgccttcta cccgaaaggc gctgtccctt aacgatcgca ccccctggat
     3601 catcaggttt tttgtgcctg caccgcagat cacagctccc atattattaa ggaagccgca
     3661 cagctggctg atttccggtt ccatggcagc accgcggatg atcgttactc cctctgcgag
     3721 cacagcagcc aaaagagcct gctccgtagc ccccacgctg ggataggaaa aaacaatctc
     3781 cgtcccctta agacgcccgt cctttcctgc gcaggcctct atgatcccgt cttcttcgcg
     3841 gatcacggcc cccattttat ttaacgccat aagatggaga tcgatgggcc ttgctccgat
     3901 cagacagcct cctggataac agcagttccc ctgccccata cggcccagaa gagctcctaa
     3961 tagaataatg gatgaacgca tggaccggac atattcctga ggcacctgcg ttttttttat
     4021 ctctccggca tcaataaaaa cactgcatcc ccggcggata cagctgcatc ccagagcttc
     4081 caggatcttc atcatacagg ctgtatcctg tatcctggga acattggtaa gaaccgtaag
     4141 acctttatgc aggagagcgg ccgccatcat gggaagggcc gcatttttcg atccctgtat
     4201 ggaaatttcc ccctttaagg gtatgcatcc actgacacgg attgcggaca aaagaccacc
     4261 tccgcagcga ttatactata caaaagtata tgccctttat ccgctgtttg tgcatttgtg
     4321 ttttgtttgc tgcatcactc atactgctgc acgtatctgg ttggatacac tgagcacgat
     4381 ccccatctct accataagaa acagaacgga tgtgcctcca taactgataa agggaagggt
     4441 aattcccgta ttggggatcg tattggtcac aacagcaata tttaaaataa cctgaatggc
     4501 aatatgggcc atcactccca caaccaaaag agcgccgaac agatccggtg catcattggc
     4561 gatcatcaca aaccggtaaa tcataagaag aaacagcatg ataaccgcaa tagccccaaa
     4621 aagacccaat tcctcacaaa tgatggcaaa aatcgtatca ttctgggatt caggaaggaa
     4681 tcccagcttc tggatgcttt cgcccagtcc ctgccccatc aggcctccgg agccaatagc
     4741 ataaagtccc tgaaggatct ggaagctctt tccctccgga tccagctccg gattcagcca
     4801 tgcctcgatc cggttatact gatacggttt aagaacatgg atatttaaaa ggaatttacc
     4861 cagaggcccg gcggtcataa ggatacccag cgcccctccg ccggccagga aaaagggcca
     4921 cttcacctta caggctacaa acagcataac aaagccgatt cccaccagga taatacctgt
     4981 actcaggtta tttgccgcta cgatcgcgcc aatggcaacc gccagcaaaa atacctttcc
     5041 cataacggcc cactggttaa tttttcctcc cagcttcgta atcactgctg ccagcatgat
     5101 gatcaacgca atttttgcaa actcagaagg ctgaaatccg atggggccta tatccagcca
     5161 tctttttttt ccgtttacct cccgtcccac caaggttaca ataaccataa gaatatgaga
     5221 caatatatag gacggcacgg ccagccggta gagccagtga tagtccatct tggaaatgat
     5281 cagcataaag ataaagcctc ccagggcgat tccaccctgg cgctgcataa aataggcact
     5341 gttattattt aggtcatcat tcagctgggc cgtataggaa ctggcgctgt agatcattac
     5401 cagtccgaaa acggtgagga caataatgat caccaccagg ctcagatcgt aaaagctgcg
     5461 ttcccttttc tttttcggaa ccgctcctgc ctcataagcc tgcttctgat tcccatactc
     5521 atatgctgca cgggccgcct gcgtccggct gccctgctgc attctctgac cctgtccctg
     5581 gcggggctct ctctgcaccc tctgggcctg aggggaacgt ccgctcggac gccttctttc
     5641 atcctgctca taccggctgt tccgggaagt cctctgcacc gtacggctgc cggtccctgt
     5701 ctttctcttt ttactgcctc cgggtctttg cgcagaccgg acgtctctca ccgaggctgc
     5761 atcataccgc gccggcactc tggccgccgc tgctcccgtt attccttccg gcctcgctgc
     5821 ccggcgctta tttccagcca ttcctttcta cctcctacag agccatcacg cattccttaa
     5881 agacgcgccc ccgttcctca aaatccttaa acatccccca gctggcacag gccgggctta
     5941 aaagcacaca gtctccggaa tcagcataaa cagcgcagac ctgcaccgcc tcctgaagat
     6001 cctccgcata cataacatct gtaaagccgt gctccctggc acaggccgca atccgatccc
     6061 tggtctgtcc gatcagcacc agatacttta ccctgccgga aaattccttg gcccagtcac
     6121 tgtaatcact gtgtttgtca taccctcctg cgatcaggag caccggcccc ggcatagcct
     6181 taagagcccg gatggcagcc tccggattcg ttcccttgga atcattataa tagcggactc
     6241 cggtgcgttc ccgcacaaac tcgatccgat gctcgaccgg cttaaactcc ctggccgctt
     6301 ctccaatcgc ctcaagggat acgccaaggg tcatggcagc agcagcggca gccatcacat
     6361 tttcatagtt atgctggcct aaaagctgaa gctccttcac atttaaaaga accgtttctt
     6421 ttccggcctt tgcatatatg atttcttctc ctttcaggaa aaatccgtcc tttaagctct
     6481 cttttgacga aaaccagatt acctttggct tcagcccctc ttttttaccg aattccttta
     6541 aaatggggtc atcaaaattc aggacaaccg tatcctcctc cgtctggttc atggtgatgc
     6601 actctttgat ctgagtatac gtttccatgg ttccgtgacg atccagatga tcaggcgtaa
     6661 tatttaaaat gacgcttaca ttgggcttaa aatccataat ggtttccagc tgaaagctgc
     6721 tgatctcagc tacggcaacc gaatcctctg tcatttctgc tgcttttgca gtaaaaggtt
     6781 cgccgatatt tcccaccaca aaagtttcct catatttttt ccttaccatc gctcccgtca
     6841 aagctgtggt ggtcgtcttt ccgtttgttc ccgtaatggc agccagcttt cctgcgctgc
     6901 actgataggc tagttcgact tctccaagga tcgggatcct ggcctcatct actactgcca
     6961 caaaaggact tgtaagagga attcccggac tgataatgca cagctccacc ccaagaaggt
     7021 cggagcgctt gatctcccct aaaacaacgt ttaccttagc gccctcttca aaccgggcct
     7081 tgatctcttt ttcatccaga gctgtattcc catcatagag aactacctct cctccccggt
     7141 taagaatcag ctttgccgca gcaatgccgc ttttgccggt tcccgcaact aatactctct
     7201 gactttgatc cattcgtggt taaacctcct tctttgccta agctgccggc gctcttacag
     7261 tcccagataa gcgaccatgc ataaaatggc cgtaacaatg gaaaatacgg ctacaaccct
     7321 tgtttcagac catccgcaca gttcaaagtg atgatggatg ggtgccattt taaagattct
     7381 ctttccccct gtctttttaa aatacgttac ctgaataata acagacagaa cctctaccag
     7441 atagatcaga ccgattatgg gaataaacag aggcagacgc atcatatatg ctgcagaagc
     7501 cacaaagccc cccagcgcca gagacccagt gtctcccatg aacacctttg ccggatatac
     7561 attaaaaagc agaaaaccca gaaggcttcc caccacagct cctgtaatgg ggctgatccc
     7621 catatcctcc ccaatggcca caatggttaa aaaagtcgct acaagtatgg ttacgctggt
     7681 gcacagaccg tccagtccat cggtaaaatt cacaccgtta tccgttccca gcgttacaaa
     7741 aaacagagcc ggaacaaaca ggatacccag agaaaggaat ttcccatcct caaacccgcc
     7801 tgtaaaggga atgagcatat cggttcccac ctcgccgctg ttaagcacat accaggcaaa
     7861 aatgcctgtg atcacgatct gtcctgccag cttctggagc ggagtcaatc cttccgaacg
     7921 cttcaggatg atcttgatat aatcgtccag aaagccgatg atcccaaaac cggccgtcat
     7981 aaaaagcacc ggaatgatct tcggataaga aggaatataa aacagggatg tgataataat
     8041 gctggataaa atgatcagtc ctcccatggt aggagttccc tgctttttta aatggctctg
     8101 aggcccgtca tcccggacct gctggccgaa tttcagcttg tgaagaaagg gaatgataat
     8161 cggacacagc agggcgctga tggcaaatgc aataataatc gctaatatcg tctcatgaat
     8221 catagggcac ctcaatctca tatgtattct gccggcaaac gccggacagc aataatagta
     8281 tacgtctttt tcggagaata atcaaccagc tttcggctcc ggccggattc ccatatatgg
     8341 aagaatattt ttaaataatt ctccgatcac gggagccgca atggttcctc cgtaatagat
     8401 tccctggggt tcgtcaatgg tgatcagggc cattaccctg ggatcgtccg ccggggcaaa
     8461 gccaagaaaa gaggaaatat attttttgag gcttcttgga agtttctctg aggtagctgt
     8521 ctttcctcca atgcggaacc cctctaacgc cgcccgtttg ccgcttccct ctgccacaac
     8581 ctgttctaat acataccgca tggaagcgct ggtctcagaa gacaggatct gctttccctg
     8641 aggataagaa agttccttta cagcacctcc atccgagcgg atcaccctta cgccaaaatg
     8701 aggcgtaacc cggcgtccgc cgtttacaat ggaagccgct gtcgtaataa gctgaatggg
     8761 tgtgatctga aaggactgtc cgaaggatac ggtagctaat tccacagcac ccattttctc
     8821 ctttttatgc atgatcgtgg ctgcctctcc cggaagatcg atccctgttt ttcccttaag
     8881 accaaactgg gtaaaatact tatagtaccg gtccactccc agccgctgtc ccacatcaat
     8941 aaacacagga ttgcaggaat tcattgctcc ctgaagaaaa gtttctgttc catggcctcc
     9001 tgccttatgg caccggatcc tccggtcttc cactacgcga aagcccggac agctgaaggt
     9061 atcctcaagc gttactgccc ccgcctccag accggctgcc gctgtgatga tcttaaaggt
     9121 agagcccggc tcatatgtat cattgatgca ggtatttctc cacatggcat tcagccggtc
     9181 ctggagcccc atggtctgcg accgggccgc cgcctcttcc ttaaggctct ggctttttaa
     9241 attctgatcc ggcgtaaaag gatcatttaa gtcaaattcc ggcgcattga ccattgcaag
     9301 aagctcgccg ttccggggat ccatcacgat cacagaaact tttttcgcat ttttttgttc
     9361 cataacctga taggcaagct gctgggcgta cagctggata tttacgtcca ggctgatctg
     9421 aagatcattt cccgccaccg gttcgatcct gtcttctgct gctttgtcaa gctccactcc
     9481 ccttgcatct gtcagcgtaa gaatcactcc gttttttccc tttaaatact tttcatacat
     9541 aacctccagg ccgataatcc cctgattgtc tcctccagta aagcccaaaa ccttggaagc
     9601 cagactgccc atgggataat accgtttata gtcctcatcc acttttactc catccagctt
     9661 taatgcccgt atcttatctc cggtttcctt atccacattt gtctttatga tctccctgga
     9721 actgcgtttt tctacctttt ttcggaccgg ctcctctgaa agctccaata tgctgcaaag
     9781 ggagcggatc acctcttccc gatccgtgac ctgattatag atcacggaaa tagtacaaac
     9841 cgtcctgtta tctgcgatca catttccccc tgcatccaga atccgtcctc tggccgcctt
     9901 gatggtacgc tctctttcat gaagatcccg ggccatctca ccatagtgat ccgcccggac
     9961 cagcataaga aaaccaagcc gccctaaaag tccggtcatc gctacagcaa tgagcaggcc
    10021 caggacggct atgttttttc tatgctttgt caaatttgga ttcatttgat aacttcccca
    10081 tccgccattg atattaaata tacggattcc cgggggattt tattcccatc aggcttcaga
    10141 ggaagctttt gtttcaccag cagtgccttc caggtcttcc ttctcttctg actggctctc
    10201 tttcttttcc ttccatccat ccgatgtttc ctctaacgac agggaaggtg cgtttggatc
    10261 tgccggtacc ccatcaggaa ggccgctttc atagccgtct cctatggttt cgtctgtttc
    10321 atataccttt tctgtttccc gtgtttcagt ctccggctct gtttcttcgc cttccgatcc
    10381 ggaaggctca ttgataccct cgtccgccgc cgtatcgcca gcctgttcct cctcagcgcc
    10441 cgcttcagga aatacattca tatatggaag ggcctctgcc ataattttgg cgaaaatgcc
    10501 gctggcaaag gagctgtgag cctgctgggg acccggagga taattgggta catccaccac
    10561 aacataggca aaaagctgcg gatcctctgc aggagcaaag cctgcgaaag acaaaagata
    10621 atttttagcc gaacgtggct gcttttctgc ggttcccgtc tttcctccaa cgtgataacc
    10681 cgctactttt gcagctcctc ccgtaccatt ttccacggtc tgaaacatgg cctctttcag
    10741 aaattccgaa gtggaagagg atacggtctc acgaaccagg accggatcct ttgtctctac
    10801 cacggaaccc tgctcattca tgatctggcg cataacatgg ggctcataat aggagcctcc
    10861 attgatgaca gaacagagtg cggcagccat ctggatcatg gtacagttgt aattctgtcc
    10921 gaaggcattg gtggcaagct ccgtcggccc catattttcc gcgctgtaga tcaggcctgc
    10981 gccatccgcc tcaccgggaa gatctactcc tgttttctgc cccattccaa aaatggcctg
    11041 gtactttgtg aaaatatcct ttcccatcat ggcgccgatc cgcatcatag ctacattgca
    11101 ggactgcatg atggactcct gaaggcttaa ggagccatgg ccgcctttta cataagccgt
    11161 acacttaatg ggccagccgc ccacatttaa aagaccttca cagtcaaaat attcatttcc
    11221 ttttaaaacg ccttcctcca gtcctgacgc tacagtaagg atcttggacg gagaaccggg
    11281 ttcataggta tcactgacac agaaatttct ccagatctgg ttccagacca cctgacgtcc
    11341 ataggagatc acatcctccc gtttcgtatc cgcgatcatc tgctcctggg tcagggttgt
    11401 atccggatgc tcccttttgt atacagaaac cgcatccttt ttcccaagct cataaagctg
    11461 gtcatcacta tagccctcga tctttctggg gtcattcaga tcaaatttat tgctggttgc
    11521 cattcccagg atctctccgt ttttgggatt catggcaatg gcagccgtca catggcttcc
    11581 cacagtagtc tgccattcat caatatactt ctggaggatg ttctggatat tcagatcaat
    11641 ggtggaaaca acggtttttc cgtttgtcgg ctcttttaac acgccttcca gattattttc
    11701 ctcatccaga tagccgtatt cacggccgtt tactcccatc agatcttcat tgtaaaactg
    11761 ctcgatcccg cccgttccca ccgtgccgtc cgcactggaa aaaccgatca cactgcaggc
    11821 ggctgaaccg tagggatata ctctccggta ctgatcctca aaccacacac ccatgatcct
    11881 taagcgtgac tggctctcat cctcacttgc ataataggcg gcattgcgct ctttgcgcat
    11941 ggtttcaaag gctaccttct gatcatagct taactgtttg ccctttttat aaggaacata
    12001 tgcccggtcg ctcttttcct ggatcaggct gcggatttcc ccggaatcaa aaccgaagca
    12061 gtccaccaga gcctgaatgg tcggctctaa atagtattcc ggctttttca tgatctgctg
    12121 gggatccagg atcaggttat acaccttttc actggtagca agcagcgttc cattgcggtc
    12181 tacaatatcc ccccgtctgt acgaaaggat ccggctggaa aattcctgct gcctctggct
    12241 taaaacgatc ttgctgtact gatcgttatt ttcctttata attccataca atacccatat
    12301 aagtgcaaat aaagccagcg tgatcacgat tgccgtgatt gccagcttcc ccctcatatg
    12361 ctctgtaaat actcttttag aatggccgct tttagatccc cttttacggc cctcagaccg
    12421 atccccataa ggggacggcc tagtgttccg ggatgtcttc atactgtctt acatactcgc
    12481 tttctgtctt atcatataag agcacctgat ctctcctggc ataaaccatg cccagctcct
    12541 tggttgcaat ctcatagatt ttattcagat caatgctgct gttgatgctg gtttccagcg
    12601 cgtcattttc cgttttcatt ttctcaagct gcgcttccag atgttcaata ttgtgcattc
    12661 tggctgtaat tccggactgc aggtgcagat agctgacaca aatatataat gtacagatac
    12721 aggcagctgc caaaagaaat acatagggca gatccatagc catggctctc tcccggttac
    12781 ggcgcagctg ctgattttga tgcagccctg tctttttccg tctgaccgga gcttttcttt
    12841 ccggtctgta ctccggttcc agctttggtg cggctgtacc cccgatataa gccatatcac
    12901 gggtatttct tctgttgtac gccccacttc tgcctgtggg tcttcccatt ctctgggcca
    12961 tggctgattc ctcccttcat aatcttctgt cctgcctgat tttcttctca ctctccccgt
    13021 tcaaacactc tcagcttcgc actctttgag cggctgtttt cctccaattc ctcctctgaa
    13081 ggaaggatgg gctttcgcgt aatcaccctg cccatgcttt tccttccgca gacacagacc
    13141 ggaaaatcgg gagggcagat acaaggatcc tcattgatcc tgaaccggct cttcacgata
    13201 cggtcttcca gagaatggaa tgtaataata ctgagtcttc ctcctgaatt taacagtctt
    13261 atcataccgt cgatggactc ctccagcaca tccagctcat gattcagctc aatgcggata
    13321 gcctgaaaag ttctcttggc gggatgaccg cctgtggctc tcaccttggc aggaatggct
    13381 gcctttatga tctcgcacag ttctcctgta gtggcaaccg gcttttcctg ccgtgccttt
    13441 acgatatgct ttgcaatgtt tttggcaaag cggtcttctc cgtaatcacg gataatgcgg
    13501 aa
//
LOCUS       DBMDUH010000063        13437 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_2432_length_13437_cov_4.464232, whole genome shotgun sequence.
ACCESSION   DBMDUH010000063 DBMDUH010000000
VERSION     DBMDUH010000063.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 13437)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13437)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13437
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2432_length_13437_cov_4.464232"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(201..>917)
                     /locus_tag="ACZPCG_06945"
     CDS             complement(201..>917)
                     /locus_tag="ACZPCG_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HIR1284839.1"
                     /translation="FLKRDLRPIVQKSLLGFASGVMVAASVWSLLIPALDMAEKTMGK
                     MSIIPASTGFLLGIGFLLLLDRLIPHLHLGEDKPEGLSKKLKKTTMLILAVTLHNIPE
                     GMAVGAVFAGIVSKNAEITLMGAFALSIGIAIQNFPEGAIISIPLRSETNMNKGKAFT
                     LGALSGIVEPIAAVCMFFLADMLESILPYILSFAAGAMIYVVIEELIPETTEGEHSNS
                     GTVGFALGFVLMMIMDVTLG"
     assembly_gap    918..927
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1112..1357)
                     /locus_tag="ACZPCG_06950"
     CDS             complement(1112..1357)
                     /locus_tag="ACZPCG_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HIR1284840.1"
                     /translation="MDKEGLLFNIDKVHTTEMGIGRIKKNLKLDTDDVVEWCKNRVLD
                     EGCNIYKQGKNWYCEIDNIKITINSYSYTIITAHIIK"
     gene            complement(1417..2163)
                     /locus_tag="ACZPCG_06955"
     CDS             complement(1417..2163)
                     /locus_tag="ACZPCG_06955"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009757281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1284841.1"
                     /translation="MEYIKVTSENIEKEHICCAISNNNDIQVSSKKAWLSERFEDGLV
                     FLKSTERGKCFIEYIPAENAWNPISADGYMYINCLWVAGSFKGHGYSADLLNACIEDS
                     KNKGKKGLCILAAAKKKPFLADPKFLKHKGFQVCDEADNGIQLWYLAFVENATLPQFK
                     ECAKHPHTDEMGYVLYYTSQCPFNAKYVPVIEGIAKEKAIQFTTIHLQSKEEAQKAPT
                     PITTYALFLDGNYITNEQMNDKRFLKLLEK"
     gene            complement(2196..2516)
                     /locus_tag="ACZPCG_06960"
     CDS             complement(2196..2516)
                     /locus_tag="ACZPCG_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HIR1284842.1"
                     /translation="MASSKEYLEFILGQLSELEEITYRAMMGEFIIYYHGKIVGGIYD
                     DRLLVKPVKSAISYMPTAPYELPYEGAKGMLLVDEVDNKNFLAGLFNAMYEELPTPKP
                     KKKK"
     gene            complement(2636..2875)
                     /locus_tag="ACZPCG_06965"
     CDS             complement(2636..2875)
                     /locus_tag="ACZPCG_06965"
                     /inference="COORDINATES: protein motif:HMM:NF018508.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HIR1284843.1"
                     /translation="MLFPLWIFIPDFIPVLGYLDDVILFPALVSLTVKFIPEDVLKKN
                     RRNAEGIWSGWNAWKWYYMIPVVAVWWIIIGVLVL"
     gene            complement(2844..2993)
                     /locus_tag="ACZPCG_06970"
     CDS             complement(2844..2993)
                     /locus_tag="ACZPCG_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HIR1284844.1"
                     /translation="MDLKKRAEKLKTDIPAIFLALKDKDTPLPAKVFAGLTIAYALSP
                     VDFHT"
     gene            complement(3164..4573)
                     /locus_tag="ACZPCG_06975"
     CDS             complement(3164..4573)
                     /locus_tag="ACZPCG_06975"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HIR1284845.1"
                     /translation="MGGFFGVVSKQSCTLDLFFGVDYHSHLGTRRGGMAVYEKGRGFA
                     RSIHNIENSPFRTKFDSDLDELTGTMGIGCISDSDPQPLLIQSHLGSYAITTVGKINN
                     EEELIRSAYENGHIHFMEMSGGRINATELVAALINQRPTITEGLLYAQEQIKGSMSIL
                     LLTEDGLYASRDRLGRTPIVIGRKEGAFCASFESFAYINLGYTDYRELGPGEIVKIHA
                     DGVETLSAPREEMKICSFLWVYYGYPTSTYEGVNVEKMRYECGKRLARRDEAQPDIVA
                     GVPDSGIAHAIGYANESGVPFARPFIKYTPTWPRSFMPQNQSQRNLIARMKLIPVDAL
                     IRGNKLLLIDDSIVRGTQLGETTEFLYQSGAKEVHIRPACPPLLFGCPYLNFSRSTSE
                     LDLITRRIIRDREGDNVSQEILDSYADPDSQNYAEMIEEIRKRLNFTTLRYHRLDDLV
                     ESVGISPCKLCTYCWNGKH"
     gene            4846..5835
                     /locus_tag="ACZPCG_06980"
     CDS             4846..5835
                     /locus_tag="ACZPCG_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HIR1284846.1"
                     /translation="MTEKKPFTLVVAKDGTGDYSTVTEALVALGEEGEPALIYIKKGV
                     YKERLTIRRPFVTFEGEDMNETVITYNLGAKMEMEDGSRRGTFRSYSVLIDTHDFTAR
                     NLTFENSAGLGALVGQALALYVDGDRILFDRCRMLASQDTIFTGPLPPKEIEPNGFIG
                     PKQYAPRINGRHYYKDCFIRGDIDFIFGSATAYFENCELYSQDIGREINGYVTAASTP
                     EGQEYGYVFEGCHFTGNCPPRSVYLGRPWRNFAKTVLLHCRMEEHIRREGWHDWGKRE
                     AHDTLYFAEAGSTGPGAVPEERPEWVRILSEDELEPYSRERVLGGNDGWMSGR"
     gene            5862..7499
                     /locus_tag="ACZPCG_06985"
     CDS             5862..7499
                     /locus_tag="ACZPCG_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002573453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR1284847.1"
                     /translation="MELYRIILVDDEEEVRKSIIRKIDWEEAGFFVAGDAENGEDALE
                     KIEALEPDVVLTDIRMPYMDGLTLAERIRQKYPSMKIVIFSGFDDFEYAKQAIKLNVT
                     EYILKPVNVEELTAILKRIKVNLDQEIEQKRNVNLLRENYIRSLPILRENFLNELVSH
                     SMDPAIVESRMREYDLDLLGAKKWIAAAIDIEEPEKERMGQLAIHQEKDLIPISVMQI
                     LEEKLGAYCRFALFNAAGVQESGLSVIAAIDGENSQTGLIDVLGDICKETRKILAVPV
                     TIGIGNNSAQLCRVSDSYKEAVNSLGYKAIVGSGSTIYINDMEPVSRGKLEFDGKTEA
                     ELGVAIKFGTKERIEASVAQIMERLNDAKVHFRQQQAYMLSVANSMIQLLQLYDLDME
                     AVFNGELKENGSLEMLPGLQKKEDFSIWLLNTALYIHQAMNRERDNTMKQVIREARQY
                     ILEHYQEPELSVEQICRHLHMSPAYFSTMFRKETGKTYVAYLTEVRLDKAVELLNKTD
                     DKTYVIAAKVGYQEQNYFSYVFKKRFGVSPTKFRGAK"
     gene            7500..9302
                     /locus_tag="ACZPCG_06990"
     CDS             7500..9302
                     /locus_tag="ACZPCG_06990"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1284848.1"
                     /translation="MKTFRQRQLPFGNGGIRSIIFLYFTITAAAASIFIGLSLYTRMS
                     GQVSETVMEENQILIDQVNRSVENYLKTVMKLSDSLYYSIIKNADLSDPSVGERFNLL
                     YENNMDQTDSIALFSADGELLESVPALRVRNNLDVTREAWFSYTLDRTENQHFFLPQV
                     QQIFESSSDQYRWVIPMTRVVEITKGTDTVQGILLIHLNYTGLKLLLDGVTLGNEGYI
                     YLIDGNGEIIYHPRAQLIDSGLEHENNRAVSEYRDGIYQETFHGEERVITVKSVGYTG
                     WKLIGVAPRQTVSLNSLKTQLLVVFVAAFILFLMSLVNSYISSRITTPIRKLELSVNE
                     IEKGNLNAKVDAEGSYEIRHLGQSVQNMAKQIQVLMADIVSEHEKKRKQEFDTLQSQI
                     NPHFLYNTLDIIVWMIENEKPDQAVKAVTALARFFRISLSRGKSIITVKDELEHVRNY
                     LMIQHMRFKNRFSYTIEAEDEVLELASLKLMLQPLVENAIYHGMEFMDGDGEIFISAW
                     KEGEDLYLKVSDNGLGMTEEQVARLFSDTPHTGSSRGSGIGVKNVNERIRLYFGSEYG
                     LSIESEPDEGTVVTIHLPAVAYSEIRQKEEHDKT"
     gene            9289..10260
                     /locus_tag="ACZPCG_06995"
     CDS             9289..10260
                     /locus_tag="ACZPCG_06995"
                     /inference="COORDINATES: protein motif:HMM:NF024799.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR1284849.1"
                     /translation="MTKREKLIWCIFGAILGVLFLLSFTDLIIKEKKAEIYPISVVLD
                     STTDECYANFKKGVEQAARDYNADVHFVTLYQEGDALQQEELIEREIKGGAQAVIVAP
                     VRELNDPERLESLAYSCPIILLGNHPSKELPVTSVIRDYEKEGELLGRAVAEQIDSVS
                     PVYVFAKEKSAGNNRPIYESFVKMLKTEGFEAVLCSRESGSDFKTVFETILYPEAPKA
                     TIAALDAESMTETAQILNSDDRYTGRLAGVFGVGSTTYLLNQMDKGLIRGLVVHNSYN
                     EGYLSVQKAAEGIKKGMKQERICLDSYYIERSDIRSRKFEKMLYPMD"
     gene            10278..11336
                     /locus_tag="ACZPCG_07000"
     CDS             10278..11336
                     /locus_tag="ACZPCG_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR1284850.1"
                     /translation="MKRYRYGLFFLTVILGAFALYRCFADRRPDHKEPESVRIGITLY
                     RDDDTFINSICRILETEAKKYEKTEGIRMILDVMDAKEDQNTQNSQVDRFLALGCDVI
                     CVNMVDRSAASVIIDKAMDREVPVIFFNREPVSEDLNRWEKLFYVGANPKEEGVCQGN
                     MIADAYDACPEILDLNGDGKVSYVILEGERGHQDSLMRTEWAVQTLKNRGVDLEKLTG
                     GIANWQRSQADALMGQWLEKFPEQIELVLSNNDDMALGAIDTLRRLGIIRPVCVAGID
                     GTPAGLDAVESGFMLGTVVIDRETYGKTMMRMALSLAETGRTPEDISLERGAYFRCPS
                     YPEIRKRKDEAPETGKIE"
     gene            11503..12705
                     /locus_tag="ACZPCG_07005"
     CDS             11503..12705
                     /locus_tag="ACZPCG_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016280059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR1284851.1"
                     /translation="MRKFRKTAAVALAAMMAFSVTGCGGGAKETTAAAAADTTAAGSE
                     AEKVSEAVTEAAKEEGKKIEDMTVGVFYYTYSDTYISSVRTALDESLKAAGIDFQDYD
                     ANSNQTTQNEQIDTAISTGANLLIVNIVTSGSVDASQAIVDKAQAAGIPVIFFNRAVE
                     SDEEEGKVLGSYDKCAFVGTDAPEAGHMQGDMIGKYLVENFDAVDLNGDGSISYAMFM
                     GQLGNVEAIYRTQYAVEDANKVLTEAGKAELQYFDSSNKDCYQVDQDGNWSATAANNY
                     MNTNLSQYNKENNNMIELIICNNDGMAEGAISALNDKGYNLGDGTSTTIPVFGVDATD
                     AAKQLIADGKMTGTIKQDAEGMAEAIAFLTQNVAEGKAVMDGTDSYNISENVSNKIYI
                     PYAAYTGE"
     gene            12803..>13437
                     /locus_tag="ACZPCG_07010"
     CDS             12803..>13437
                     /locus_tag="ACZPCG_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012200261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1284852.1"
                     /translation="MDKEVLLQMTDICKEFPGVKALDGVSLTVKRGTVHALMGENGAG
                     KSTLMKCLFGIYNKDSGHIYLEGNEINFKNSKEALEHGVAMVHQELNQALKRSVMDNL
                     WLGRYPRKGVMIDEHKMYEDTKKLFNDLGIDVDPRRIMSTMPVSQRQMLEIAKAVSYD
                     AKVIVFDEPTSSLTEQEVEHLFDIINMLRDKGCGIIYISHKMAEILRISDEV"
BASE COUNT         3901 a         2766 c         3223 g         3537 t
ORIGIN      
        1 aaaacagtat ttgtgaattt ttctttttta gtcgcttttt gtgtcttatc taacacgaga
       61 gaaataacag ggacaacgaa tacagcaaat ataatagttc ttatcataaa cagcactcct
      121 tacaatttct aattttgtct atttttttca gtataccata cgaaatatcc ttttgtttgg
      181 gaaactcccc tctttttcat ttaacctaga gtcacgtcca taatcatcat aagcacaaat
      241 ccaagtgcaa atccaactgt tccgctgttt gaatgctctc cttccgtcgt ttccggaatc
      301 aattcctcta ttacaacata aatcattgcc cctgctgcaa aagataaaat atacggtaaa
      361 atcgattcca gcatatccgc aagaaaaaac atacatacag ccgcaatcgg ttcaacaatt
      421 ccggaaagcg ctccaagtgt gaatgctttt cccttattca tatttgtttc acttcttaac
      481 ggtatggata taattgctcc ttccggaaaa ttttgaatag caatcccaat cgacaatgca
      541 aacgcaccca tcagtgtaat ctctgcattt ttgctaacga ttccggcaaa aacagctcct
      601 actgccattc cctccggaat attatgcaga gtgacagcaa gtatgagcat agttgttttc
      661 tttaatttct tcgacagccc ttccggctta tcttctccta agtgcagatg tggaatcaac
      721 cgatccaata acagtaaaaa tcctattccc aataaaaatc ctgttgatgc cggaataatt
      781 gacatctttc ccattgtttt ttctgccatg tcaagcgccg gaatcagtaa tgaccacaca
      841 gacgcagcta ccataacacc tgaagcgaat cctaataaag atttctgcac tatcggtctc
      901 aaatctcttt ttaagaannn nnnnnnnttc ctttcaatca aaatattcat cacaaatttc
      961 tttcacaact aaaacagggt atctttctac tgaaaagcag tcaatcctaa gcctaaccac
     1021 ttgtatagat gcaaaactcc ctgtcgatag ttagttgtga ctaactatat tatacttctt
     1081 atactgaaaa aagtaaatat gaaactggaa attatttgat tatgtgtgct gtaataattg
     1141 tgtaactgta agagttaatt gttattttaa tattatcaat ttcacaatac caattctttc
     1201 cctgcttgta gatattgcac ccttcatcta gcaccctatt cttacaccac tcaaccacgt
     1261 catcggtatc taatttgaga tttttcttta ttctaccaat tcccatttcc gtagtatgaa
     1321 ctttgtctat attgaataac aaaccttctt tatccatatg ctatccccca taaatctcgt
     1381 tttattgcta cgttcatttt tcgataaacc ggaagtttac ttctctaata atttcaaaaa
     1441 tcgcttgtcg ttcatctgtt cattcgtgat gtagttgcca tctaaaaaca atgcatatgt
     1501 tgtgattggt gtcggagctt tttgtgcttc ctctttactc tgtaaatgaa ttgtagtgaa
     1561 ttgtattgct ttttcttttg caatcccctc aatcacagga acatacttcg cattaaatgg
     1621 acattgactt gtataataca aaacataacc catttcatca gtatgggggt gtttagcaca
     1681 ttctttgaat tgtggcaatg tggcattctc tacgaatgct aaataccata actgaatacc
     1741 gttatcggct tcatcacata cttgaaatcc tttatgtttc agaaatttag gatcagctaa
     1801 aaatggcttc ttctttgccg ctgctaaaat acacaagcct tttttacctt tatttttact
     1861 gtcctcaata caggcattta ataaatcggc tgaataacca tgccccttaa atgaacctgc
     1921 aacccataag caattaatat acatataacc atctgctgaa atcggattcc atgcattttc
     1981 agcagggata tattcaataa agcactttcc tctttctgtg cttttcagaa aaaccaaacc
     2041 atcttcaaat cgttctgaca gccatgcttt ttttgaagat acttgaatgt cattgttatt
     2101 agaaatcgca cagcatatat gttctttttc tatattttcg ctcgtcacct taatatattc
     2161 cattatataa aacctccaca aaaattcaaa attgtttatt tcttcttttt cggctttgga
     2221 gttggtaact cctcatacat agcattaaac aagcctgcta aaaaattctt attatcaact
     2281 tcatctacca acaacatccc ttttgctccc tcatagggta attcatacgg agctgtcggc
     2341 atataactaa ttgctgattt tactggttta acaagtaatc tatcatcata gataccgcct
     2401 acaatcttgc catgataata aataataaat tctcccatca tagctcgata agtaatttct
     2461 tctaactcag atagctgccc taaaataaac tctaagtatt ccttgcttga tgccatattt
     2521 ccacctcaaa ttctaattgt cctatttttc ctttgcactt ctaaaaacaa agaaaaatcc
     2581 tacgcaacca atcaataatc ctaaaagaga aaaaggagta tcgctacact tcattttaca
     2641 acaccaatac cccaattata atccaccaca ctgccacaac cggaatcata taataccatt
     2701 tccaggcgtt ccagccgctc cagattccct cagcatttct cctatttttt ttcagaacat
     2761 cctcaggaat gaatttgacc gtcaatgata ccagtgcagg aaacagtatc acatcgtcca
     2821 gatatcccag aaccgggata aaatcaggta tgaaaatcca caggggaaag agcataggct
     2881 atggtaaggc cggcaaatac ctttgcggga agcggcgtat ctttgtcttt cagcgccaaa
     2941 aatatagcag gaatatctgt ttttaatttc tctgcccgtt ttttaagatc cattctcttt
     3001 cctcctgctg tgttctgtga atttggtata ttcaggccga ttttatcaca gttttaaagc
     3061 aaaatccata cagatataaa aataaaagcg ctcccgtata tcatgatctg tgtacgggag
     3121 cgttttaaat cattttaaat catattgcga tacggcggta aaatcaatgc ttcccgttcc
     3181 agcaataggt acagagctta cagggagaga ttcccacaga ttccaccaga tcatccagac
     3241 ggtggtagcg gagtgtggta aaattcagcc gtttgcggat ttcttcgatc atttccgcat
     3301 aattttggct gtcagggtct gcatagctgt caaggatctc ctgagataca ttgtctccct
     3361 cacggtcgcg gatgatccgt cgggtgatca gatccagctc agaggtagaa cgggaaaagt
     3421 tcaaatacgg gcagccaaag agcagtggtg ggcaggcagg acggatatgt acctcttttg
     3481 ctccgctctg gtagagaaat tctgtggttt ctcctaattg tgtaccccgg acaatggaat
     3541 cgtcaatcag cagaagcttg tttccccgga tcagagcatc tacggggatc agcttcattc
     3601 tggcgatcag gtttcgctgg ctctgattct gaggcataaa ggatctgggc caggtaggtg
     3661 tatatttgat aaagggacgg gcaaagggaa cgccggattc attggcatag ccaatggcgt
     3721 gagcgatacc ggaatcaggc acacctgcca caatgtccgg ctgggcttca tcccggcggg
     3781 ctaaacgttt tccgcattca tagcgcattt tttctacatt cacaccttca taggtagagg
     3841 tgggataacc gtagtaaacc cagaggaagg agcagatctt catttcttct ctaggagcgc
     3901 ttaaggtttc tacgccgtct gcatggattt ttacgatctc tcccggaccc agctcccggt
     3961 aatcagtata gcctaagttg atgtaggcaa agctttcaaa ggatgcacag aaggcgcctt
     4021 cttttcgacc gatcacaatg ggggtacgtc caaggcggtc tctggatgcg taaaggccgt
     4081 cttctgtaag aaggagaata ctcatggagc ccttgatctg ctcctgagca tagagaagcc
     4141 cctctgtgat ggtaggtctc tgattgatca aagcagccac cagctccgta gcattgatgc
     4201 gcccgccgct catttccata aaatggatgt gaccgttttc ataagcggat cggatcagtt
     4261 cctcttcatt gttgatcttt cctactgtgg taatggcata actgcccaga tgggactgga
     4321 tcaacagggg ctggggatcg ctgtcagaga tacagccgat ccccatcgtg cctgtaagct
     4381 catccagatc gctgtcgaac tttgtgcgga agggggaatt ttcaatattg tgaatggatc
     4441 tggcaaagcc ccttcctttt tcatatacag ccatgccccc tcgtctggtt ccaagatgtg
     4501 aatgataatc tacgccaaaa aacagatcca gcgtacagct ctgttttgaa acaactccaa
     4561 aaaaaccgcc catagagatt acttcctttc tgtaaagaat ccatgtatgt atcataacat
     4621 gatgtgttct tgtttgactc tgatatcata acatatatta aaataaaata acaatagggt
     4681 atatttatta tttatactta tcaaacatat aagaaaggga aaaagaaaca ggggaaggga
     4741 aaatcaaaaa cggccggaat tgcaaaaaat gacaattcat tgtataataa ttccaaatac
     4801 aaaggataaa aataggacaa ttttatcaga caggagagag aagatatgac agaaaaaaag
     4861 ccctttaccc ttgtggtagc caaagacgga accggagatt attccactgt tacagaggca
     4921 ttggtagctc tgggagaaga aggggagccg gcgcttatct acataaaaaa aggtgtatat
     4981 aaggaacgtc tgactatccg ccggcccttt gtaacgtttg agggcgagga tatgaatgaa
     5041 acagtgatca cctataatct gggagccaag atggagatgg aagatggaag ccgcagagga
     5101 accttccgca gctattctgt cttaattgac acgcatgatt ttacggccag aaatctgacc
     5161 tttgaaaaca gcgccggact gggggcatta gtgggacagg ctctggccct ttatgtggat
     5221 ggagacagga tcctgtttga ccgctgccgg atgctggcca gccaggacac catttttaca
     5281 ggtccccttc cgccaaagga aatcgaacca aacggtttta tcggccccaa gcagtatgcc
     5341 cccaggatca atggccgtca ttattacaag gactgcttta tcaggggaga tattgatttt
     5401 atttttggaa gcgccacggc atattttgaa aactgtgagc tgtattccca ggatattggc
     5461 cgggagatta acggatatgt gaccgcggca tctactcctg agggacagga atatggctat
     5521 gtatttgagg gctgccattt tacaggcaac tgtcctccaa ggagcgttta tctgggccgt
     5581 ccatggagaa attttgcaaa aaccgtgctg ctgcactgcc gcatggagga gcatatccgc
     5641 agagaaggat ggcatgactg gggcaaacgg gaggcgcatg acaccctgta tttcgccgag
     5701 gcaggaagca cgggccctgg agctgtgcct gaggagcgtc cggaatgggt aaggattctt
     5761 tctgaagatg agctggaacc ttacagccgg gagcgcgtcc ttggaggaaa tgacggctgg
     5821 atgagcgggc gataagacag actgggagag gggaactgca tatggaatta taccgtatca
     5881 tcctggtgga tgatgaagaa gaagtaagaa agagcattat ccgaaagatt gactgggaag
     5941 aggccggttt ttttgtggca ggagatgcgg aaaacggaga ggatgcgctg gaaaagatcg
     6001 aggcattgga gccggatgta gtgctgaccg atatccgtat gccctatatg gacggcctta
     6061 cccttgcaga gcggatccgg cagaaatacc cctccatgaa aatcgttatt ttttcggggt
     6121 ttgatgattt tgagtacgcc aagcaggcca ttaagctcaa cgtgacagag tacattttaa
     6181 agcctgtgaa tgtggaggaa ctgacagcaa tcttaaaacg gatcaaggtg aatctggatc
     6241 aggaaatcga gcagaagcgg aatgtaaacc ttttgagaga gaactatatc agaagccttc
     6301 ctattttaag ggaaaacttt ttaaatgaac ttgtaagcca ttccatggat ccggcaatcg
     6361 tggaaagccg gatgcgggaa tacgatctgg atcttctggg agccaagaag tggatcgctg
     6421 ctgcgattga catcgaggag ccggaaaagg agcgcatggg ccagctggcc attcatcagg
     6481 aaaaggatct gatccccatt tccgtgatgc agattttgga ggaaaagctg ggtgcttact
     6541 gccgttttgc tctttttaac gcagccggtg ttcaggaatc cggtctgtcg gtcattgccg
     6601 ccattgacgg ggaaaacagc cagacaggcc ttattgatgt actgggagat atctgtaagg
     6661 aaaccagaaa gatcctggct gttcctgtta ccatcggcat tggaaataat tccgcccagc
     6721 tttgcagggt cagcgattcc tataaggagg cggttaattc tctgggctac aaggccattg
     6781 tggggagcgg gagcaccatc tatatcaacg atatggagcc ggtgagcaga ggtaagctgg
     6841 agtttgacgg caagacagag gcagaacttg gcgttgcgat caaatttggc actaaagaga
     6901 ggattgaggc gtcggtggcg cagatcatgg aacggttgaa tgatgcaaag gttcatttcc
     6961 gccagcagca ggcgtatatg ctgtcggtgg caaattctat gatccagctg ctgcagctgt
     7021 atgatttgga tatggaggct gtgtttaatg gagagttaaa ggaaaacgga agccttgaga
     7081 tgctccctgg cctgcagaaa aaggaggatt tttccatctg gcttttaaat acagcgctct
     7141 atatccacca ggccatgaac cgggaaagag acaataccat gaagcaggtg atccgggaag
     7201 cgagacagta tattctggag cactatcagg agccggagct gtccgtggaa cagatctgcc
     7261 gtcatctgca tatgagcccg gcatattttt ccactatgtt ccggaaggaa acgggaaaaa
     7321 catatgttgc ctacctgaca gaggtgcgtc ttgacaaggc ggtggaactt ttaaataaga
     7381 ctgatgacaa gacctatgtg atcgcggcaa aggtaggata ccaggaacag aactatttca
     7441 gctatgtgtt taaaaaacgt ttcggggtat ctcccacgaa attcagggga gcaaagtaaa
     7501 tgaaaacatt cagacagcgg cagctgccct ttgggaatgg cggtatacgt tctattattt
     7561 tcctgtattt tacaattaca gcagcagctg ccagtatttt tattggcctc tctctgtata
     7621 cgagaatgtc tgggcaggtt tctgaaacag taatggagga aaatcagatc ctgatcgatc
     7681 aggtaaaccg ttctgtggaa aattatctga aaacggttat gaagctgtct gattccctgt
     7741 attacagtat tattaaaaat gcagacctgt cagatccgtc cgtcggtgaa cggtttaacc
     7801 tgctttatga aaataatatg gatcagacag atagtatcgc tcttttttcg gctgatgggg
     7861 aactgctgga atcggtaccg gctcttcgtg tgcgaaacaa tctggatgta acaagggagg
     7921 catggttttc ctatacgctg gatcggacgg agaatcagca tttttttctt ccgcaggttc
     7981 agcagatttt tgagagcagc agtgatcagt accgctgggt gatccccatg acgcgggtgg
     8041 tggagattac gaagggtacg gatacggttc aggggatttt gctgatccat ctgaattata
     8101 caggacttaa gctgcttttg gacggcgtta ctctgggaaa tgaagggtat atttatctca
     8161 tagacggaaa cggagagatc atttatcatc caagagccca gctgatcgat tcgggacttg
     8221 aacatgaaaa taatcgggct gtatcggaat acagggatgg gatctatcag gaaacctttc
     8281 atggagaaga aagggtaatt accgtaaaat cagtcggcta cacaggctgg aagctgatcg
     8341 gcgtagcccc caggcagacc gtttccctca acagccttaa gacccagctt ctggtggttt
     8401 ttgtggctgc ctttatcctc tttcttatgt ctcttgttaa ttcctatatt tcatccagaa
     8461 tcactacacc catacgaaag ctggagcttt ctgtcaatga aattgaaaaa ggcaatttga
     8521 atgcaaaggt ggatgcggaa ggatcttatg agatccggca tctgggccag tcagttcaga
     8581 atatggcaaa gcagatccag gtgctgatgg cagatatagt atcggagcat gaaaagaaac
     8641 gaaagcagga atttgatacg ctgcagtctc agattaatcc ccatttcctc tataataccc
     8701 tcgatattat tgtgtggatg atcgaaaatg aaaaaccaga tcaggcagtg aaagcggtta
     8761 ctgctctggc gcgttttttt cggatcagct taagccgcgg aaagagcatt attacagtga
     8821 aagatgaact ggaacatgtg agaaactatc ttatgatcca gcatatgcgg tttaaaaacc
     8881 ggttcagtta taccatagag gcggaggatg aggtactgga acttgccagc ctgaaactga
     8941 tgcttcagcc gttggtggaa aatgccatct accatggcat ggagtttatg gatggcgacg
     9001 gggaaatctt tataagcgcc tggaaagagg gagaggatct gtacctgaag gtgtctgata
     9061 atggcctggg catgacagag gaacaggtgg cccggctctt ttctgatacg ccccataccg
     9121 gctcgtccag aggttccggg attggagtga aaaatgtaaa tgagcggatc cggctgtatt
     9181 ttggcagtga atatggactt tccattgaat cagaaccgga tgagggaact gtggtaacga
     9241 tccatcttcc ggcagtggca tacagcgaga tccgtcaaaa ggaggagcat gacaaaacgt
     9301 gaaaaactga tatggtgtat cttcggtgct attttgggag ttttattcct tctttccttc
     9361 acagatctga tcataaagga aaaaaaggcg gagatttatc ctatatcggt tgttctggat
     9421 tctaccacag atgagtgcta tgccaatttc aaaaaggggg tagagcaggc agccagagac
     9481 tataacgccg atgtgcattt tgtaacgctt tatcaggagg gggatgccct gcagcaggag
     9541 gagcttattg aacgggagat caaaggagga gcgcaggcgg tgatcgtagc tcctgtaaga
     9601 gagctaaatg atccggaacg tctggaatca ctggcatata gctgccctat cattcttttg
     9661 gggaatcatc cttcaaaaga gcttcctgtt acctccgtga tcagagatta tgaaaaggag
     9721 ggggaacttc tgggacgtgc ggtagcggaa cagattgaca gcgtatcgcc ggtttatgtg
     9781 tttgcgaaag aaaagtccgc tggaaataat cgtcctattt acgagtcttt tgtaaaaatg
     9841 ctgaaaacag aaggctttga agccgtgctt tgcagcaggg aaagcggctc tgattttaaa
     9901 acagtttttg aaaccatcct ttatccggaa gctccgaaag caacgatagc ggccctggat
     9961 gcagaaagca tgacagagac agcacagata ttaaatagtg atgaccggta tacgggccgt
    10021 ctggcaggtg ttttcggggt gggaagcacc acgtatctgt tgaatcagat ggataaggga
    10081 ctgattcggg ggctggtagt tcataacagt tataatgagg gatatctgtc cgttcagaaa
    10141 gcggctgagg ggataaaaaa gggcatgaaa caggaacgta tctgtcttga ttcctactat
    10201 atagaacgtt cggacatccg cagccgtaaa tttgaaaaaa tgctttatcc catggattag
    10261 ccgcaaggag ggggaaaatg aaacgataca gatatggact gtttttttta acggtgattc
    10321 ttggggcttt tgcgttatac cgctgttttg cagaccggcg cccggatcac aaagaaccgg
    10381 aatcggtgcg catcggcatt actctttaca gagacgacga tacgtttatc aattcaatct
    10441 gccgtattct ggaaacagaa gcaaaaaagt atgagaaaac ggaaggaata cggatgatcc
    10501 tggatgtaat ggatgccaag gaggatcaga atacccagaa tagccaggtg gaccggttcc
    10561 tggctttggg ctgtgatgtg atctgtgtca atatggtaga ccggtctgct gcttctgtga
    10621 tcattgataa ggctatggac cgggaggtcc cggtgatttt ttttaaccgg gaacctgtga
    10681 gtgaggattt aaaccgctgg gaaaaacttt tttatgttgg ggccaatccg aaggaagagg
    10741 gggtctgcca ggggaatatg attgccgacg cctatgacgc ctgtcctgaa attctggatc
    10801 tgaacgggga tggaaaggta agctatgtga ttttggaagg agagagaggg catcaggatt
    10861 ctcttatgag gacagaatgg gctgtgcaga cattaaagaa ccgcggggtg gatctggaaa
    10921 aactcaccgg ggggatcgcc aactggcaaa gaagccaggc agatgccctt atggggcagt
    10981 ggctggagaa atttccggag cagattgagc tggtgctcag caacaatgac gatatggctt
    11041 tgggggctat tgatacgctg cgccgcctgg gcattatccg gccggtgtgt gtggcaggga
    11101 ttgacggcac tccggctgga ctggatgcag tggagtctgg ttttatgctt ggaacagtgg
    11161 tgatcgacag agaaacttat ggaaaaacca tgatgcgcat ggcgttgtct ctggcagaga
    11221 caggcaggac gccggaggac atcagcctgg aaagaggagc atatttcagg tgtccttcct
    11281 atcctgagat acgaaagagg aaagatgaag cgccggagac aggaaaaata gaataaaatc
    11341 acaaaaaaat gaagaaaaat atcgaaaaaa gaagaaatca aataaattcg ataatgtgga
    11401 aaataaaaat gtattgaaaa atgcataaaa acattctaaa atatatcttg tcaaacggac
    11461 atggtgtcca ataaaaacaa acaaatggga ggagttacaa cgatgagaaa attcagaaaa
    11521 acagcagctg ttgctctggc agccatgatg gcattttccg taacaggatg cggaggcgga
    11581 gcaaaggaaa caaccgcggc tgcagcagct gacacaaccg cagcaggatc tgaagctgag
    11641 aaggtaagcg aggcagttac agaggccgca aaggaagagg gcaagaagat tgaggatatg
    11701 accgttggtg tattctatta tacatacagc gacacatata tttcaagcgt ccgtaccgcc
    11761 ctggacgagt ccttaaaggc agctgggatt gatttccagg attatgatgc aaacagcaat
    11821 cagactacac agaatgagca gatcgatact gcgatcagca ccggtgccaa tcttctgatc
    11881 gtaaatatcg ttacctccgg ttctgttgac gcttctcagg ctattgtaga caaggcacag
    11941 gcggcaggca ttcctgttat cttctttaac cgtgccgttg agtctgatga ggaagagggc
    12001 aaagtacttg gaagctatga caagtgcgca tttgtaggaa ccgatgctcc cgaggcaggc
    12061 catatgcagg gcgatatgat cggtaagtat ctggtagaga actttgatgc cgtagattta
    12121 aatggtgacg gaagcatcag ctatgcaatg ttcatgggcc agttaggaaa cgtagaggca
    12181 atttaccgta cacagtatgc tgtagaggat gcaaacaagg ttctgacaga agctggaaaa
    12241 gctgagcttc agtactttga ttcctccaac aaggactgct atcaggttga ccaggacggc
    12301 aactggagcg caactgcagc aaacaactat atgaacacca acctgtccca gtataataaa
    12361 gagaacaaca acatgatcga gctgatcatc tgcaataacg atggtatggc agagggcgct
    12421 atctccgcat taaatgataa gggttacaac cttggcgatg gaaccagcac cacaattcct
    12481 gtattcggcg tagatgctac agatgctgca aagcagctga tcgcagacgg caagatgaca
    12541 ggtaccatca agcaggatgc tgaaggtatg gcagaggcaa ttgcattcct gacacagaac
    12601 gtggcagaag gcaaggctgt tatggatgga acagacagct ataacatttc tgagaatgta
    12661 agcaacaaga tctatatccc atacgctgct tatacaggcg aatgatgaaa gtctgatttt
    12721 gaaagcggcc tctgccccaa gcggcagggc cgctttttaa aaggaataca cgcagtaaaa
    12781 ggaaagcggg gagggaagct gtatggataa agaagtgctg ctgcaaatga cagatatctg
    12841 taaggagttt ccgggtgtaa aggctctgga cggcgtttct ctgacagtaa aaagagggac
    12901 tgtccatgct cttatgggtg aaaacggcgc tggtaaatct acgttgatga agtgcctgtt
    12961 cggcatctat aataaggaca gcggacatat ttatctggaa ggaaatgaaa ttaactttaa
    13021 aaattcaaaa gaggctctgg aacatggtgt agccatggtg catcaggagc tgaatcaggc
    13081 gttaaagcgc agtgtaatgg ataacctgtg gctggggcgc tatccccgca agggagttat
    13141 gattgacgaa cacaaaatgt atgaggatac aaaaaagctg tttaacgatc tgggaatcga
    13201 tgtagatccc agacggatta tgagcaccat gcctgtatcc cagcgtcaga tgctggagat
    13261 cgcaaaggcg gtatcctatg acgcaaaggt gatcgtattt gacgaaccca cctcatccct
    13321 tacggagcag gaggtagagc acctctttga tatcatcaat atgcttcgtg acaagggctg
    13381 cggcattatt tacatttccc ataagatggc agaaatcctg cgtatttctg atgaagt
//
LOCUS       DBMDUH010000064        13408 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_2438_length_13408_cov_6.401593, whole genome shotgun sequence.
ACCESSION   DBMDUH010000064 DBMDUH010000000
VERSION     DBMDUH010000064.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 13408)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13408)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13408
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2438_length_13408_cov_6.401593"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="ACZPCG_07015"
     CDS             complement(<1..542)
                     /locus_tag="ACZPCG_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HIR1284853.1"
                     /translation="MSDIMTPIPFGKLMNWILEEHKKGSIFGIKKPFAADPKKMSTIF
                     GRGLETPFGPAAGPHTQLTQNIVASYVAGSRFFELKTVQKLDGEDLPVSKPCIKADDE
                     CYNCEWSTELYVPQAYDEYIKAWFACKVLAREYGLGSMDGFQFNMSVGYDLEGIKTEK
                     IDRFIEGMKDASEAPIFAECR"
     gene            complement(696..2027)
                     /gene="ssnA"
                     /locus_tag="ACZPCG_07020"
     CDS             complement(696..2027)
                     /gene="ssnA"
                     /locus_tag="ACZPCG_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HIR1284854.1"
                     /translation="MLMIGNGRLVTRDAEQPFFENGAVVIDGTTVKKVGDLETLKKEF
                     PDAEFVDAKGGVIMPAFINTHEHIYSAMARGLSINGYDPKGFLDILDGMWWTIDRHLT
                     NEQTRQSARVTYMDCIKNGVTTVFDHHASFGEIKDSLFAIEDAAKEFGLRTCLCYEIS
                     DRDGMDKARESVMENARWIRHALADDTDMIAGMMGMHAQFTISDETMDLAAANKPEEV
                     GYHIHVAEGIEDLHDCLKKYGKRIVDRLMDKNILGSKTLLGHCIYVNPHEMELIKETD
                     TMVVHNPESNMGNACGCPPTMEIVHRGILTGLGTDGYTQDILESYKVANVLHKHHLCD
                     ANAAWGEVPQMLFEGNAKIANRYFKKQLGVLKEGAAADVIVTDYIPRTPMNASNVNSH
                     ILFGMTGRSVVTTVCNGKILMKDRELLGIDEEKVLYEVRNAAADLAKSING"
     gene            2262..2915
                     /locus_tag="ACZPCG_07025"
     CDS             2262..2915
                     /locus_tag="ACZPCG_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR1284855.1"
                     /translation="MAYTLNLLKQIADGLAKQFGPDCEIVIHDLKRHDLEHSVVHINN
                     GHVTNRREGDGPSKIVLETLHKDPALLKDHVGYLTRTSNGKILKSSTFFIRNEEDTAI
                     DYIFAINYDITGLMTVDRSIRSLIDTEQGSSPKQQPEQIVHNVNDLLDTLIEQSVALV
                     GKPAALMNKEEKVTAIQFLNDAGAFLITKSGDKVSKYFGISKFTLYSYIDVNSRKSS"
     gene            2944..4152
                     /gene="dpaL"
                     /locus_tag="ACZPCG_07030"
     CDS             2944..4152
                     /gene="dpaL"
                     /locus_tag="ACZPCG_07030"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HIR1284856.1"
                     /translation="MDQKILWAVNHMPKTEDKNLPIMGLEEIKKARAFHESFPQYSQT
                     PLTKLPNMASYLGVKEVYVKDESYRFGLNAFKVLGGSFAMARYIAKETGKDVSELPYS
                     VLTSEKLREEFGQATFFTATDGNHGRGVAWAANRLGQKAVVHMPKGTTQTRLENIAKE
                     GAQVDIQEMNYDDCVRLAAKEADETPRGVIVQDTAWDGYEEIPAWIMQGYGTMAMEAG
                     EQLKAQGCERPTHIFVQAGVGSLAGAVVGYFANLYADNPPTFVVVEAEAAACLYKGAA
                     AGDGDIRIVDGDMQTIMAGLACGEPNTISWDILKNHVKVFIAAPDWVAANGMRMLAAP
                     IKGDASITSGESGAAPFGALAAMTTLDEYEQLKKDIGLDENSRVLLFSTEGDTDPDRY
                     KNIVWKGLDK"
     gene            4184..5500
                     /locus_tag="ACZPCG_07035"
     CDS             4184..5500
                     /locus_tag="ACZPCG_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HIR1284857.1"
                     /translation="MNLDYAKIKEAAQNYQKDMTKFLREIVKNPGESCDEKAHIERIA
                     EEMRNLGFDKVEIDPMGNVLGFMGTGETLIGFDAHIDTVGIGNRDNWTFDPYEGYETE
                     TEIGGRGVSDQCGGIVSAVYGAKIMKDLGLLSDKYTVLVTGTVQEEDCDGLCWQYIIN
                     EDKYRPAFVVSTEPTDGGIYRGQRGRMEIRVDVKGVSCHGSAPERGDNAIYKMADILQ
                     DVRALNENDAADDKEVKGLVKMLDQKYNPEWEEANFLGRGTVTVSEIFFTSPSRCAVA
                     DSCSVSLDRRMTAGETWESCLDEIRALPSVKKYGDDVTVSMYEYSRPSYKGLVYPIEC
                     YFPTWVIPKDHEVTKALEEAYTNLFGENRIGAEETLEMRTARPLTDKWTFSTNGVSIM
                     GRNSIPCIGFGPGAEAQAHAPNEKTWKQDLVTCAAVYAALPTVYCK"
     gene            5592..6785
                     /gene="ygeW"
                     /locus_tag="ACZPCG_07040"
     CDS             5592..6785
                     /gene="ygeW"
                     /locus_tag="ACZPCG_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006776489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HIR1284858.1"
                     /translation="MKTLQDYIDKLNSLNFKEMYEGDFFLTWEKTDDELEAVWTLADA
                     LRFMRENNISTKVFESGLGISLFRDNSTRTRFSFASACNLLGLEVQDLDEGKSQVAHG
                     ETVRETANMISFMADVIGIRDDMYIGKGNAYMHEVVDAVTQGYKDGILEQKPTLVNLQ
                     CDIDHPTQCMADMLHIIHEFGGVENLKGKKLAMTWAYSPSYGKPLSVPQGVIGLMTRM
                     GMEVVLAHPEGYEVMPEVEEVARKNAEKSGGSFRVSHDMADAFKDADIVYPKSWAPFA
                     AMEKRTNLYAEGDSDGIKALEKELLAQNAEHKDWCCTEELMKTTKDGKALYLHCLPAD
                     INDVSCKDGEVEASVFDRYRDPLYKEASYKPYVIAAMIMLAKFADPADILKKLEDRGT
                     PRVFK"
     gene            6954..7916
                     /gene="arcC"
                     /locus_tag="ACZPCG_07045"
     CDS             6954..7916
                     /gene="arcC"
                     /locus_tag="ACZPCG_07045"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002575487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HIR1284859.1"
                     /translation="MENKKKKRIVIALGGNALGNTLPEQMKAVKITAKAIVDLIEEGC
                     EVIVAHGNGPQVGMINNAMAALSREDANQPNTPLSVCVAMSQAYIGYDLQNALREELY
                     NRDIYDIPVATMITQVRVDADDPAFDTPSKPIGHFMTEEQAKVAEEKYGYIMKEDAGR
                     GYRRVVASPKPAEIVEIGAIRSLVDSGQLVIACGGGGIPVTRQGNHLKGASAVIDKDF
                     ASELLAENLDADFLIILTAVEKVAINFGKPEEKWLNELDTEEARKYMKEGHFAPGSML
                     PKVQAAVKFAESKPGRTALITLLEKAKDGIQGKTGTHIHLGQNN"
     gene            8036..9367
                     /locus_tag="ACZPCG_07050"
     CDS             8036..9367
                     /locus_tag="ACZPCG_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015851 - nucleobase transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease family protein"
                     /protein_id="HIR1284860.1"
                     /translation="MNTAKKHASLFELDGIPKMSQAIPLALQHVVAMIVGCVTPAIII
                     SGAAGIDTADRVLLIQASLVVSALATLLQLFPIGSKNGFHLGSALPVILGVSFAYVPS
                     MQAIAETGGIPAILGAQIVGGICAILVGLSIKKIRRFFPPLIAGTVVFTIGLSLYPTA
                     INYMAGGTGQPGYGSWQNWTVAIFTLIVVTVLNHFAKGFLKLASILIGMIAGYIFSSF
                     FGMVSFSNVAQAGVFQLPEVMHFGIEFEPSACVALGLLFVINSVQAIGDFSATTAGGL
                     DREPTTGELHGAILGYGVSNILGAFLGCLPTATYSQNVGIVATTKVVNRVTLGLSALI
                     ILTAGLFPKFSALLTTIPYAVLGGATVSVFASIAMTGMKLVMTENMNYRNTSIVGLAA
                     ALGMGVSQASASLASFPDWVTTIFGKSPVVIATLVAVFLNVTLPKDKAAEK"
     gene            complement(9486..10220)
                     /locus_tag="ACZPCG_07055"
     CDS             complement(9486..10220)
                     /locus_tag="ACZPCG_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HIR1284861.1"
                     /translation="MAAAVLFAAVMAGGCATPAQFPDTIKVQDVNDSEGITVTGKEEV
                     KVVPDMTEIRYGVYSQAQTASQCQEDNAKQLDQTIETLKGLGVKETSIQTSAYGLSPI
                     YDWNSGNRTITGYEMNTEITVSDILIDQAGEILSQSVTAGVNQIESVTYFSSQYDESY
                     QEALKGAVAMARSKAEAMAEAFGKKLSSVVDIQEYGYNPQARYSSYNAPAAKMAVEET
                     AAASDMAVMPGEVSVEAQVTVTYGLE"
     gene            complement(10380..11246)
                     /locus_tag="ACZPCG_07060"
     CDS             complement(10380..11246)
                     /locus_tag="ACZPCG_07060"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIR1284862.1"
                     /translation="MADIRMITDIRDPALDVYSRTPEVQLLRLFEPNPGIFIAESPKV
                     VQRALDGGYEPISILAEKGKMEGEADEVIKRCGDIPVYMAEPDVLTGLTGFKLTRGLL
                     CAMKRKSLPSVREICAGARRIAVLENVVNPTNVGAVFRSAAALNMDAVLLTPGCSDPL
                     YRRAARVSMGTVFQVPWTKFDEKKEPWPEGGMELLKELGFKTAAMALKQDSIFLDDPS
                     LRAEEKLAVILGTEGDGLASDTIADCDYTVMIPMSHGVDSLNISCASAVAFWELGKRD
                     ADRGSRKNRKTI"
     gene            complement(11376..13403)
                     /locus_tag="ACZPCG_07065"
     CDS             complement(11376..13403)
                     /locus_tag="ACZPCG_07065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HIR1284863.1"
                     /translation="MELKESELQSGERKETFRRILLYMDYGNVPDWENELAAGKTVEI
                     SGRPEVVKGPLNPGEFDFRNYYRSKGIAVKIYGKTYFGSEGSAYPCPAMLERIKEHCA
                     QVLKKCCTPKDAAVFMAMLLGDSGEMEKEQRELYRDSGIAHLLAVSGQHLSVIGGGVY
                     LILRKLGFGFTGAGAVGAFLVLNYGLLTGGSGSAMRAVIMILCLWLAKKEGRTYDTLS
                     ALGMAAVLLLWQQPYLVFQSGFQLSFLAVWAIAGPGMGLCRLLGLEKGWQKGAAVSLC
                     IQMILTPVMLWHYFKYPFYGMALNVLILPFVPMLMVSGLLVIGLGGFSTALGRGAGGA
                     GHYILGYYDWLCSQSRKFPGYSLVLGRPGWERIAVYVLFMIVLFAGLRVWNGKKGKTG
                     RSVKLALFFCAFYFSLVIFIPMPVRGIGVLCMDVGQGDGFLLQAGTHSVLIDGGSSSE
                     KKLGEMTLEPCLNSMGIKRLDVSVVSHGDNDHISGLMYLLEQKTVCPGLLLLPAGGKG
                     QEIYERLERLQKEAGGKTQYMETGDVLRVGELKLTCVYSGEGSDQTDRNAHSLVICAD
                     SGDFHMLFTGDMGKREEEQLLIQADRREGAWIREHLAHINLLKTAHHGSSGSSCLAFL
                     EAVTNVRLAVISYGKGNSYGHPDPGVVKRMEHLRIKVLTTGGKGSIRIWVP"
BASE COUNT         3388 a         3564 c         3043 g         3413 t
ORIGIN      
        1 cggcattctg caaaaatcgg agcctcagaa gcatccttca tgccttcgat aaaacggtcg
       61 attttctctg tcttaatgcc ctctaagtca tatcccacag acatattaaa ctggaacccg
      121 tccatagagc caaggccgta ctcccttgcc agcaccttgc aggcgaacca cgccttgata
      181 tactcatcat atgcctgagg cacatagagc tctgtagacc actcacagtt atagcactca
      241 tcatctgcct tgatacaggg tttggatacg ggaagatcct cgccgtctaa tttctgcacg
      301 gtctttaact caaagaagcg gctgccggcc acataggatg ccacaatatt ctgtgtaagc
      361 tgcgtatggg gacctgctgc aggtccgaag ggagtttcaa gacctcttcc gaaaatagtg
      421 ctcatcttct ttggatcagc ggcaaagggc tttttaattc cgaaaatgga gcccttctta
      481 tgctcttcaa gaatccagtt catcagtttt ccaaaaggaa tgggtgtcat aatgtcgctc
      541 atatttttcc tccttcacac gaccaggtcc cagcaccgga aacatccatt ttccttctct
      601 tgtccggctc agccgcccgg ccataaaaga ccggggtgct gctttcccgc cgccctgtaa
      661 tcggcacttg ttttattttc tttgatggat tataatcagc cgttgatgga ttttgccaga
      721 tctgcggctg cgttccttac ttcatagaga accttctctt catcaatgcc aagcagctcc
      781 ctgtccttca tcaggatctt tccgttgcag accgtagtca caacgctgcg tccggtcatg
      841 ccgaataaga tatggctgtt tacattggat gcgttcattg gggttcttgg tatgtaatct
      901 gttacgatca catctgcggc tgcgccctcc tttaatacgc ccagctgctt tttaaaatag
      961 cggttggcaa tctttgcatt gccctcgaag agcatctgcg gaacctcgcc ccaggccgcg
     1021 ttagcgtcac agagatgatg cttgtgaaga acatttgcca ccttgtaaga ctctaatata
     1081 tcctgagtat agccgtctgt tccaagtcct gtcaggatgc ctctgtgaac aatctccatg
     1141 gttggaggac agccacaggc attgcccata ttggactcag gattgtgcac taccatcgta
     1201 tccgtttcct taattaattc catttcatga ggatttacat agatgcagtg gcccagaagg
     1261 gttttgcttc ccagaatatt tttgtccatc agacggtcta caatgcgctt tccgtatttc
     1321 tttaaacagt catggagatc ctcaattccc tcagctacat ggatgtgata gcctacctcc
     1381 tcaggcttgt tggcagctgc cagatccatc gtttcatcag agatggtaaa ctgtgcatgc
     1441 atgcccatca taccggcaat catatctgta tcatctgcaa gggcatggcg gatccatctg
     1501 gcattttcca tcacagactc tcttgccttg tccatgccgt cacggtctga aatctcatag
     1561 cacaggcagg tacgcagacc aaactccttc gcagcgtcct cgatggcaaa caggctgtcc
     1621 ttgatctcgc caaagcttgc gtgatggtca aatacggtag tcacgccatt tttgatgcag
     1681 tccatatagg taactctggc actctggcgt gtctgctcat tggtcagatg gcggtcgatg
     1741 gtccaccaca ttccgtccag gatatctaaa aatccttttg gatcatatcc gttaatgcta
     1801 aggcctcttg ccatggcgct gtagatatgc tcatgggtat taatgaaagc cggcatgatg
     1861 acgcccccct ttgcgtcaac aaactcggcg tcagggaact cttttttcag ggtttccaga
     1921 tcgcccacct tcttaactgt ggttccgtcg atcactactg ccccgttctc aaaaaatggc
     1981 tgttcggcgt ctcttgtaac aagacgtcca tttccgatca tcagcatatg gtactctctc
     2041 cttttttgtt ttgttaccct tatagtaaca ctataaaagt ccatttgcaa gggtaaaata
     2101 caattttttt tagttataca aaattttttt gtaaaaacct cttgcaatta catttttttg
     2161 tgctatgatg gaatcataaa caatgcggtt gcattttgca aagtatcatt gtacaaagta
     2221 accatataca catcatctag aatatgacgg gaggtaaggc tatggcatat actctgaatc
     2281 tgctgaagca gatcgccgac ggccttgcaa agcagttcgg acctgactgc gagatcgtga
     2341 tccacgactt aaaacggcac gatctggaac attcggtcgt acatatcaac aacggccatg
     2401 tgacaaaccg cagggaaggc gacggccctt caaagatcgt cctggagacg ctccacaagg
     2461 accctgcttt gttaaaggat catgtaggct atctgacgcg cacatcaaat ggaaagattt
     2521 taaaatccag cacattcttc ataagaaatg aagaggatac cgctatagac tacattttcg
     2581 ccatcaatta tgatatcacc ggccttatga ccgttgaccg ctccatccgc tctcttattg
     2641 ataccgagca gggaagcagt ccaaagcagc agccggaaca gatcgtacat aatgtaaatg
     2701 atctgctcga tacactgatt gaacagtccg tagcactggt gggcaaaccc gccgctctga
     2761 tgaataagga agaaaaggta acagcgattc aatttctcaa cgatgcaggc gcttttctta
     2821 ttacaaaatc aggcgacaag gtttcaaagt attttgggat ctccaagttt accctgtaca
     2881 gctacatcga tgtaaacagc cgcaaatcat cttaaaccac acaaataagg aggacaaagt
     2941 atcatggatc agaaaattct gtgggcggtc aaccacatgc cgaagacaga agacaaaaat
     3001 cttcccatca tgggattaga ggagattaaa aaggcaagag cgttccacga aagcttcccg
     3061 cagtacagcc agactcctct tacaaaactt ccaaacatgg cttcctatct gggagtaaag
     3121 gaagtttatg taaaagatga gtcttaccgt ttcggcctta atgcatttaa ggtacttggc
     3181 ggctccttcg caatggcgcg ctacattgca aaggaaacag gaaaggatgt ttccgaactt
     3241 ccctactccg ttcttacctc tgaaaaatta agagaggaat ttggtcaggc taccttcttt
     3301 acagctaccg acggaaatca cggacgcggc gttgcatggg ctgcaaaccg tctgggtcag
     3361 aaggctgtcg tacatatgcc aaagggcacc acacagaccc gtcttgagaa catcgcaaaa
     3421 gagggcgctc aggtagacat tcaggaaatg aactatgatg actgtgttcg tctggctgcc
     3481 aaggaagcag atgagactcc aagaggcgtg atcgtacagg ataccgcatg ggacggctat
     3541 gaagagattc ccgcatggat catgcagggc tacggcacca tggcaatgga ggccggcgag
     3601 cagttaaagg ctcagggctg cgagcgtcct acccacatct tcgtacaggc cggcgttggt
     3661 tccttagcag gcgctgtcgt aggctacttt gcaaaccttt atgcagataa ccctccaacc
     3721 ttcgtagtag tggaagcaga agctgctgcc tgcctctaca agggcgctgc tgccggagac
     3781 ggcgacatcc gcatcgttga cggcgatatg cagaccatca tggcaggtct tgcctgcggc
     3841 gagccaaata ccatttcctg ggatatctta aagaaccatg taaaggtatt tattgctgct
     3901 cctgactggg tagctgcaaa cggtatgcgt atgctggccg ctccgatcaa gggcgacgct
     3961 tccatcacct ccggtgagtc cggcgcagct ccattcggcg ctctggcagc tatgactaca
     4021 ctggatgaat acgagcagtt aaagaaggat atcggactgg atgagaactc cagggttctt
     4081 ctcttctcca cagagggcga taccgatccc gaccgctaca aaaacatcgt atggaagggc
     4141 ttagacaaat aattttaaaa tcaattatgg aggatgatat aaaatgaatc tggattacgc
     4201 aaaaatcaaa gaagctgcac agaactatca gaaagatatg accaagttct tacgcgagat
     4261 cgtaaagaac ccaggcgaga gctgtgatga gaaggctcac atcgagcgca tcgcagaaga
     4321 gatgagaaac cttggctttg acaaggtaga gattgatccc atgggcaacg tactgggctt
     4381 tatgggtaca ggcgaaaccc ttatcggctt tgacgcacac attgataccg taggcatcgg
     4441 caaccgtgac aactggacct tcgatccata cgaaggctat gagaccgaaa ctgagattgg
     4501 cggccgtggc gtttccgacc agtgcggcgg tatcgtatcc gcagtatacg gcgctaagat
     4561 catgaaggat ctgggacttc tctctgacaa gtacaccgtt cttgtaaccg gtaccgttca
     4621 ggaagaggac tgcgacggtc tttgctggca gtatatcatc aatgaggaca aataccgtcc
     4681 tgcattcgtt gtttccaccg agcctaccga cggcggcatc taccgcggac agagaggccg
     4741 tatggagatc cgtgtagatg ttaagggtgt ttcctgccat ggttccgctc ctgagcgcgg
     4801 tgacaacgca atctacaaga tggcagatat ccttcaggat gtccgcgcat taaatgaaaa
     4861 tgacgcagca gatgacaagg aagttaaagg acttgtaaag atgctggatc agaagtataa
     4921 tcctgagtgg gaagaggcta actttctcgg ccgcggaacc gtaaccgttt ccgagatttt
     4981 cttcacctcc ccaagccgct gtgcagttgc agactcctgc tctgtatcct tagaccgccg
     5041 catgaccgca ggcgagacct gggaaagctg cttagacgag atccgtgctc ttccatccgt
     5101 taagaagtac ggtgatgacg taactgtttc catgtatgag tattcccgtc cttcctacaa
     5161 gggtctggtt taccccatcg agtgctactt cccaacctgg gtaatcccga aggatcatga
     5221 agtaacaaag gcgttagagg aagcttacac caacctgttc ggcgagaacc gtatcggtgc
     5281 tgaggagact ctggagatga gaaccgcacg tcctctgact gacaagtgga ccttctctac
     5341 caacggcgta tccatcatgg gccgtaacag catcccatgc atcggctttg gaccaggcgc
     5401 tgaggctcag gcacacgcac caaacgagaa gacctggaag caggatctgg taacatgtgc
     5461 tgccgtatac gctgctcttc caaccgttta ctgcaagtaa tttcatatca tctgccgtcc
     5521 gcggtctgaa acgatcggac tgcgggggca tttatctcat actcattcta tcaaaaagga
     5581 gaacaaaaca catgaaaact ttacaggatt acattgataa actgaattcc ttaaacttca
     5641 aggaaatgta tgagggcgat ttcttcctta cctgggagaa gacagatgac gagttagagg
     5701 ctgtatggac tctggctgac gctcttcgtt tcatgagaga aaacaacatt tccacaaagg
     5761 tatttgaaag cggtctggga atctccttat tccgtgacaa ctccacccgt acacgttttt
     5821 ccttcgcttc tgcctgcaac ctgttaggtc ttgaggttca ggatctggac gagggcaaga
     5881 gccaggtagc tcacggcgag actgtccgcg agactgcaaa catgatctct ttcatggctg
     5941 atgtaatcgg tatccgcgat gatatgtaca tcggcaaggg aaatgcatat atgcatgagg
     6001 ttgttgacgc tgttacccag ggctacaagg acggcatcct ggagcagaag ccaactctgg
     6061 ttaacttaca gtgcgatatc gaccatccta cccagtgtat ggctgatatg ctgcacatca
     6121 tccatgagtt cggtggcgta gagaacttaa agggcaagaa gctggctatg acatgggctt
     6181 actctccttc ctacggaaag ccattatccg ttcctcaggg tgtgatcggc ctgatgacac
     6241 gtatgggtat ggaagttgtt ctggctcatc cggaaggcta cgaggttatg cctgaagtag
     6301 aggaagttgc aagaaagaac gctgagaagt ccggcggttc cttccgcgta tcccacgaca
     6361 tggctgacgc attcaaggat gctgatatcg tttatccaaa gagctgggct ccatttgcgg
     6421 ctatggaaaa gagaactaac ctgtacgctg agggcgattc cgatggcatc aaggctctgg
     6481 agaaggagct gctggctcag aacgcagagc acaaagactg gtgctgtaca gaagagctga
     6541 tgaagaccac caaggacgga aaagctctgt acctgcactg tctgcctgct gacatcaacg
     6601 atgtaagctg caaggacggc gaggttgagg catctgtatt cgaccgctac cgtgatcctc
     6661 tgtacaaaga agcaagctat aagccatacg taatcgctgc tatgattatg ttagctaagt
     6721 tcgctgatcc tgccgacatc ttaaagaaac tggaagacag aggtactcca agagtattca
     6781 agtaattttc tgaactaata ttgccaggct gccggaccgg agcgcgcctt tacattgctt
     6841 gtgtcagacg caatttcagg cgtacgccgg gaccggcagc tctttttaac ccatatttcg
     6901 ttttttgatc agcaaagata ctaatttttg gtaatacgag gggaatttta actatggaaa
     6961 ataagaagaa aaaacgcatt gtcatcgccc tgggaggaaa tgcactggga aatacccttc
     7021 cggagcagat gaaggccgta aagatcactg caaaagcgat cgtagacctc attgaggaag
     7081 gctgcgaagt aatcgttgcc cacggaaacg gtcctcaggt cggcatgatc aacaacgcca
     7141 tggctgcctt aagccgtgaa gacgccaatc agcccaacac acctctctct gtctgcgtag
     7201 ccatgagcca ggcttacatc ggctatgacc tgcagaacgc cttaagagaa gaactttaca
     7261 accgcgatat ctatgatatt ccggtggcta ccatgatcac tcaggtccgt gttgatgcag
     7321 atgatcctgc ttttgatact ccctccaagc caatcgggca ctttatgacc gaggagcagg
     7381 ctaaggttgc agaagaaaaa tacggctaca ttatgaagga agacgcagga cggggctacc
     7441 gccgtgtagt agcttcccca aagcctgctg aaattgttga aatcggagcc atccgctctc
     7501 tggtagactc cggtcagctt gtaatcgcct gtggcggagg aggtatcccc gtaacccgtc
     7561 agggcaatca cttaaagggt gccagcgctg ttatcgataa agattttgca agtgagctgt
     7621 tagcagaaaa tctggatgca gatttcctca tcatcctcac tgctgtagaa aaagttgcga
     7681 tcaacttcgg aaagccggaa gaaaaatggc tgaatgagct tgatacggaa gaagcaagaa
     7741 agtatatgaa agagggtcat tttgctcccg gttccatgct tcctaaggtt caggctgctg
     7801 ttaaattcgc tgaatcaaaa ccaggacgca ctgccctcat taccctgctt gaaaaggcaa
     7861 aggacggtat ccagggcaaa accggtaccc acattcactt agggcagaat aactaaataa
     7921 ttttgcatac agataacctg ttcatccggc tgctgggatg gacgggtatt tgtggggggt
     7981 caaaagtctg ctatggcagc ctcctgtata ctaaaaagta aaggaaaaca acattatgaa
     8041 tacagcaaaa aaacatgcgt ctctttttga actggacgga ataccaaaga tgagccaggc
     8101 cattcctctg gctctgcagc acgttgttgc tatgatcgtg ggctgtgtaa cgcctgccat
     8161 catcatttcc ggcgcagccg gtattgacac tgccgaccgc gtgcttttaa tccaggcttc
     8221 ccttgtggta tccgctctgg caacgctttt gcagctcttc cccatcggaa gcaaaaacgg
     8281 ctttcatctc ggctctgccc ttcccgttat cctgggagta agctttgcct atgtacccag
     8341 tatgcaggcg attgctgaaa caggcggtat tcccgccatt ctgggcgctc agatcgtagg
     8401 cggtatctgc gcgatcctgg taggtctgtc catcaaaaag atccgcagat tcttcccgcc
     8461 tctgatcgca ggaacagttg tatttaccat cggcctctca ctctatccta ctgccatcaa
     8521 ttacatggcc ggcggaaccg gccagcccgg ctacggctcc tggcagaact ggacggttgc
     8581 catcttcact ctgatcgttg taaccgttct caaccatttt gcaaagggat ttttaaaact
     8641 ggcttctatt ttaatcggaa tgatcgcagg ctacatcttt tccagctttt tcggaatggt
     8701 aagcttttcc aacgtggcac aagccggtgt attccagctt cccgaagtca tgcactttgg
     8761 catcgaattt gagccctctg cctgcgtagc cttaggtctg ctctttgtca tcaattccgt
     8821 acaggccatt ggagacttct ctgctactac ggccggcggt ttagaccgtg aaccaaccac
     8881 cggcgagctt cacggagcca tcctcggata cggtgtttcc aatatactcg gtgcattttt
     8941 aggctgcctt cccaccgcta cctacagcca gaatgtaggt atcgtagcta ccacaaaggt
     9001 cgtaaaccgc gtgactctgg ggctttctgc actgatcatc ctgaccgcag gacttttccc
     9061 aaaattttcc gcccttttaa ctacgattcc ctatgcggtt ttaggcggcg ctaccgtatc
     9121 tgtgttcgca tccatcgcca tgaccggcat gaagcttgtt atgacggaaa atatgaacta
     9181 ccgcaatacc tccatcgtag gtcttgcagc cgctctggga atgggtgttt cacaggcctc
     9241 tgcctctttg gcttccttcc cggactgggt aactaccatt ttcggtaaat cgccggtagt
     9301 tatcgctact ctggttgctg ttttcttaaa cgtaacgctt ccaaaagaca aggcggcaga
     9361 gaaataaatt gaatctgtat acagcaacgt gcgccgcaag gcgcactaaa aaaggctccg
     9421 tccatccgct ttgaatggcc ggagcctttt tttgctcagt aatcctcttg tctctttcgt
     9481 tatccttact ccagcccata ggtcactgta acctgggcct ctacactgac ctctccgggc
     9541 attaccgcca tgtctgaagc tgcagccgtt tcttccactg ccatcttggc tgctggggca
     9601 ttgtaggagg aatatcgggc ctgcggattg tagccgtatt cctgaatatc taccacagaa
     9661 gaaagcttct ttccaaatgc ctccgccatc gcttctgcct ttgatcttgc catagcgaca
     9721 gcgcccttta acgcttcctg atagctctca tcatattggc ttgaaaaata ggtgacagac
     9781 tcgatctgat tcacacctgc tgtcacagac tggctgagga tctctcctgc ctggtcaatg
     9841 agaatatccg ataccgtgat ctctgtattc atctcatagc ctgtgatcgt ccggtttcct
     9901 gagttccagt cataaatggg actcaagcca taggcagatg tctgaataga agtttccttc
     9961 acccccagtc cttttaaggt ttcaatggtc tgatccagct gttttgcatt gtcctcctgg
    10021 cactgggaag cagtctgggc ctgactgtag actccatagc ggatctcggt catatccgga
    10081 accaccttta cctcttcctt ccctgtaact gtgattccct ctgaatcatt tacatcctga
    10141 accttaatcg tgtccgggaa ctgagccgga gttgcgcagc cacctgccat tacagccgca
    10201 aacagtacag ccgccgccat tcctgcccgg ttccggattt gttttccgct caaacctctc
    10261 ttttttcgct ctgcaatgta tctatctcgt ttcatctaag tcttcctccc gtcattggca
    10321 cgcctcttct ttctgccttt attctaccag attcagggaa agatggtatt tcttttcgct
    10381 taaattgtct tacgattttt cctggaacct ctgtcagcat ccctttttcc cagttcccag
    10441 aatgccactg cactggcgca gctgatattc agcgaatcca ccccatgact catggggatc
    10501 atcaccgtgt aatcacagtc tgcaatggta tcagacgcca gtccgtctcc ctccgttcca
    10561 aggatcaccg ccagcttttc ttctgctctg agagacggat catccagaaa aatagaatcc
    10621 tgctttaagg ccatagccgc agttttaaat cccagttcct ttaaaagttc cattcctcct
    10681 tctggccatg gctctttttt ctcatcaaat tttgtccagg gaacctgaaa taccgttccc
    10741 atgctgactc tggccgcacg gcggtacaaa gggtcgctgc agccgggagt caaaagcacc
    10801 gcatccatat ttaacgctgc agcagaacgg aatacagcgc ctacattggt aggatttacc
    10861 acattttcaa gaactgcaat ccttctggct ccggcacaga tctcacggac agacggaagg
    10921 gatttccgct tcatagcaca taaaagtcct cttgtaagct taaagcccgt aagtcccgta
    10981 agtacatccg gttctgccat ataaaccgga atatccccgc accgcttaat gacctcatca
    11041 gcctcacctt ccattttccc tttttctgcc aaaatagata tgggctcata gcctccgtcc
    11101 aaagcgcgct ggactacctt tggactttct gcgataaaaa ttcccggatt tggctcaaac
    11161 agacgcagaa gctgaacctc cggcgtcctg gagtacacat ccagagcggg atcccttata
    11221 tccgttatca ttctaatatc tgccatgtta aattctcctg tgttctaagg taaaatagcg
    11281 attctttgac caggatattt tctgctcccg gttcaatcat acgcaccgtt ttattatgga
    11341 aaatggaacc ttttttgtca actgccattt cggttttatg gaacccagat ccggatgctc
    11401 ccctttccgc cggtcgtaag taccttaatt ctcagatgct ccatcctctt tacgactccc
    11461 ggatccggat ggccgtagga atttcctttc ccgtaggaaa taaccgccag ccggacgttc
    11521 gtaacagctt ccaaaaaggc cagacaggat gagccggaag aaccgtgatg agccgtcttt
    11581 aatagattga tatgggccag atgctccctg atccatgctc cctctctgcg gtctgcctgg
    11641 atcagaagct gctcctcttc cctttttccc atatcccccg taaacagcat atgaaaatct
    11701 ccactgtctg cgcatatcac cagcgagtgg gcgttacggt ctgtttgatc gctgccctct
    11761 ccactgtaaa cacaggtaag cttaagctca cctacacgga gcacgtctcc cgtttccata
    11821 tactgggttt tccctcctgc ttctttctga agccgttcca gacgttcata gatctcctgt
    11881 cctttccctc cggcaggaag aaggagaagc cccggacaaa ccgttttctg ctccaaaaga
    11941 tacataaggc cgcttatatg gtcattatcc ccatggctca ctacagaaac atccaatcgt
    12001 ttgattccca tactgtttaa acagggttcc agcgtcatct ctcccagttt cttttcactg
    12061 ctgcttcctc cgtctataag cacgctgtgg gttcctgcct gcaaaaggaa tccgtctccc
    12121 tgtcccacat ccatacacag aacgccaatt ccccttacag gcatagggat aaagattaca
    12181 aggctgaaat aaaaggcgca aaaaaacagc gcaagcttca ctgacctgcc agtcttcccc
    12241 ttcttcccgt tccaaacccg cagtcccgca aacagaacga tcataaagag cacatacacc
    12301 gctatccttt cccatccggg tcttccaagt accagactgt atcccggaaa cttccgggac
    12361 tggctgcaca gccagtcata ataccccagt atataatgac ctgcccctcc agcccctctt
    12421 cccaaagctg tgctgaagcc tccaagcccg atgaccagaa gacccgaaac catcagcatc
    12481 ggcacaaagg gaagaatcag tacatttaac gccataccat aaaaaggata tttaaaatag
    12541 tgccacagca tcacaggcgt aaggatcatc tgaatgcaca ggcttacagc ggcgcccttc
    12601 tgccagcctt tttccagccc cagaagcctg caaagcccca ttcccggccc ggcaatggcc
    12661 catacggcaa gaaaggaaag ctgaaatccg ctctgaaata ccagataggg ctgctgccac
    12721 aaaagcagca ctgccgccat ccccagtgca gagagggtgt cataggtcct tccctctttt
    12781 ttagccagcc acagacagag gatcatgatc acagcacgca tggcagagcc ggagcctccg
    12841 gtgagaaggc catagttcag gaccaaaaag gccccaacag ctccggctcc cgtaaagcca
    12901 aatccaagct tccttaagat cagataaacc ccgccgccga tcacggacag atgctgtccg
    12961 cttacagcca gaagatgagc gatcccgctg tcccgataca gctccctctg ctccttttcc
    13021 atttcgcctg aatcgcctaa cagcatggcc ataaacacag ccgcatcttt cggagtacag
    13081 cacttcttca agacctgagc acagtgctcc tttatccgtt caagcatggc cggacaggga
    13141 tacgccgaac cctcagagcc aaaatatgtt ttcccatata tctttacggc aattcctttt
    13201 gaacggtaat agttacgaaa atcgaactct cccggattga gaggcccttt taccacctca
    13261 ggccgtcctg aaatctccac cgttttccct gctgccagtt cattttccca gtccggcaca
    13321 ttgccataat ccatatacaa cagtatacgg cgaaacgttt ccttcctctc tccgctttgg
    13381 agctctgatt ctttaagctc cagtgtca
//
LOCUS       DBMDUH010000065        13258 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_2465_length_13258_cov_9.137417, whole genome shotgun sequence.
ACCESSION   DBMDUH010000065 DBMDUH010000000
VERSION     DBMDUH010000065.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 13258)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13258)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13258
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2465_length_13258_cov_9.137417"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="ACZPCG_07070"
     CDS             <1..330
                     /locus_tag="ACZPCG_07070"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043364 - glycyl-radical enzyme activating
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HIR1284864.1"
                     /translation="HEKLCSQPQQPVMAFESYLEEKKIPVWIRHVVVPGITDQEEYLL
                     RLGQYLGMRSNIKALDVLPYHDMGKVKYKNLGIPYPLEHTAPLSKEAAAKARQIILKG
                     MRESRGL"
     gene            455..622
                     /locus_tag="ACZPCG_07075"
     CDS             455..622
                     /locus_tag="ACZPCG_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005540980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HIR1284865.1"
                     /translation="MAHVITDACVSCGTCAGECPVNAISEGDGKYVIDADACIDCGTC
                     AAACPTGAIEA"
     gene            699..1424
                     /locus_tag="ACZPCG_07080"
     CDS             699..1424
                     /locus_tag="ACZPCG_07080"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006771173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HIR1284866.1"
                     /translation="MTPVKPIIGITPSHNTENDDTSLRPTYPRAIRAAGGLTILLPLE
                     GTDEDWRQLAAMCDGFLFSGGPDLHPFLFGDETHSACGNVSPVRDSMEIRLLKAAMEL
                     KKPILGICRGIQLLNVALGGTIYQDIPSQMERTFPLAHKQPFYYTVPSHHVDIKKGSL
                     LSKASGGLERISVNSQHHQAIKDAAPGLIPVGWAPDGVIEAVEKPDYPFFLGVQWHPE
                     YLWQNDPAAIGLFRLFVEACKAS"
     gene            complement(1426..2226)
                     /locus_tag="ACZPCG_07085"
     CDS             complement(1426..2226)
                     /locus_tag="ACZPCG_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284867.1"
                     /translation="MKQLTGREDEKMDSFLRNALKEQAETLAIGPEQTERMCRGVYQR
                     IEEEGSMRKTWNMRKTAILVAAVCLVGAMTAVAAGQVKTAISSSSHAEDFTEYSQLAE
                     KEEQLGLKTKAPEAFSNGYRFAYGTPVYSRGEDEQGNVVKEQQDLSLMYSKENMADLS
                     LNIEHGNLFDESDAPDQTAVHEGITLKYSQDHYRMVPPDYEPSAEEKAQEAAGELVIS
                     YGTDRVIDQTAQTLTWEDNGQFYTLLGFDVEMTPEEFYQMAGEIIDAE"
     gene            complement(2223..2789)
                     /locus_tag="ACZPCG_07090"
     CDS             complement(2223..2789)
                     /locus_tag="ACZPCG_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007705031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR1284868.1"
                     /translation="MDEQWAKSLACRVAAGDREAFDELMRHFYPGLLRAAYLIAGNRA
                     DSEDIVQETFTACWINRMRIRDPEHIGKWLYRTMTREAWRVGRRNRREQPVEEVLSED
                     TSGNGSVLDDVVNASGRQELFAAIAALPVKQRTVLVLYYYNGMSTREIAAVAGCLEGT
                     VKSRLFTARKNLKTALTKERAMGREALL"
     gene            complement(2996..3652)
                     /gene="thiE"
                     /locus_tag="ACZPCG_07095"
     CDS             complement(2996..3652)
                     /gene="thiE"
                     /locus_tag="ACZPCG_07095"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HIR1284869.1"
                     /translation="MKFDRRQLMVYAVTDRAWTGKMSLCEQVEAALKGGATMVQMREK
                     GLTNDTVQDYLEEARSLRALTERYKVPLIIDDNIKLALLCGADGVHVGQNDMDAAQAR
                     ALLGPDKILGVTAKTVEQALKAQEQGADYLGSGAVFGTSTKADALPMTMERLGEICRS
                     VSIPVVAIGGICLENIGKLKGSGAAGAAVVSGIFGAEDIEGTVRQMREMVGEWGRHCS
                     "
     gene            complement(3680..4987)
                     /gene="thiC"
                     /locus_tag="ACZPCG_07100"
     CDS             complement(3680..4987)
                     /gene="thiC"
                     /locus_tag="ACZPCG_07100"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003501053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HIR1284870.1"
                     /translation="MEYTTQMDAARKGIVTEELRKVAEKEHFEVNELMALVAEGKAAI
                     PANKNHRCLDPNGIGSMLRTKINVNLGTSRDCKDLDMELEKVNYAVGMGAESIMDLSS
                     WGDTQKFRRKLTAECPAIIGTVPIYDAVVYYHKPLKDITTEEWLKIVEMHAEDGVDFM
                     TIHVGINRRTAQRFKENKRLMNIVSRGGSIIFAWMEMTGKENPFYEHYDRILEICREH
                     DVTISLGDACRPGCIDDATDISQIEELVTLGELTRRAWEKDVQVIVEGPGHMPLNQIE
                     ANMKIQQTICKGAPFYVLGPLVTDVAPGYDHITAAIGGAIAASSGASFLCYVTPAEHL
                     RLPDLNDVKEGIIASRIAAHAADIAKGIKGAADWDRQMSLARKKLDWEKTFELSIDPE
                     KARNYRASAKPEKEDTCSMCGNFCAVKNMNRILDGEIVSIFDE"
     regulatory      complement(5169..5278)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(5340..6037)
                     /locus_tag="ACZPCG_07105"
                     /pseudo
     CDS             complement(5340..6037)
                     /locus_tag="ACZPCG_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516951.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(6231..6629)
                     /locus_tag="ACZPCG_07110"
     CDS             complement(6231..6629)
                     /locus_tag="ACZPCG_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR1284871.1"
                     /translation="MIFVGGVSQGQKILSEGLVRLCGSCGSHGRYQVIMTYMYFSFFF
                     IPLFKWNRRYYVKMDCCEAVYELDPVVGKAVLRGENPDIAEADLRLVQAGRYAKTWQE
                     GSKKPHKKCMRCGFETDEDYNYCPVCGGRI"
     gene            complement(6952..8676)
                     /gene="ilvD"
                     /locus_tag="ACZPCG_07115"
     CDS             complement(6952..8676)
                     /gene="ilvD"
                     /locus_tag="ACZPCG_07115"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009254938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - L-valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HIR1284872.1"
                     /translation="MKELNSQRVRALAPENDPLKIGMGWTVEDLDKPQILVESTFGDS
                     HPGSAHLDQFVEEAMRGITDAGARGARYYTTDICDGIAQGHDGINYSLAHRDMIANMI
                     EIHGNSTGFDGGVFIASCDKSVPACLMGLARLDMPSIVITGGVMDAGPDLLTLEQIGM
                     YSAMCQRGEITDEKLTYYKHHACPSCGACSFMGTASTMQIMAEALGLMLPGSALMPAT
                     CEDLKEMAYKAGKQVMELVKKGIKVSDIVTEKAFENAIMVHAAISGSTNSLLHIPAMA
                     HELGIEIDADTFDRMHRGAHYLLDIRPAGKWPAQFFYYAGGVPAVMEEIKSMLHLDVM
                     TVTGKTLGENLEDLKKNGFYDKCESYLKKWGLKRTDVIRTFDEPIGTDGTVAILRGNL
                     APEGSVVKHSAVPKEMFKAVLKARPFDCEEDAIAAVLSHKIRPGDAVFIRYEGPKGSG
                     MPEMFYTTEAISSDPELGKTIALITDGRFSGASKGPAIGHVSPEAADGGPIALVEEGD
                     LIRIDIPERVLEIVGIHGQEMTKEEVDKVLAERRAAWKPKAPKYTKGVLKIYSEHAVS
                     PMKGGYMV"
     gene            complement(8917..9636)
                     /locus_tag="ACZPCG_07120"
     CDS             complement(8917..9636)
                     /locus_tag="ACZPCG_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HIR1284873.1"
                     /translation="MGGFSNIKSKLLAEQVQEQIYHYILETPLEPGSKLPNEFELGEK
                     FDVGRSTIREAVKLLVSQGILEVRRGSGTYVVNTTPSDLDPLGLSGAEDKMALALDLV
                     NVRLILEPGIAEMAALNATEEAILELRCCCERVEERIASGDMYIQDDIAFHTSVARCS
                     GNKVVEQLIPIIDTAVMMFVNVTHQRLTKETVMTHRAIVDAISDRDPIGAKTAMMMHM
                     TYNRNMILKLIREEKEKNHKE"
     gene            complement(9668..11428)
                     /locus_tag="ACZPCG_07125"
     CDS             complement(9668..11428)
                     /locus_tag="ACZPCG_07125"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HIR1284874.1"
                     /translation="MNRRTARILGAAAAGTAALVTLRELKKVRHRKEEERQAAIDAAL
                     VRNRDYGSRRAYLIGGGLASMAAAAYLIRDCNFPGSHITIYEGTHILGGGNDGAGTPE
                     KGYVCRGGHMLNEKTYENFWELFDTIPSLKKTGRSVAEEIQEFAHSHSGRTHGRLADR
                     EGTFLNVKSMGFDQGDRMALFRLLLTDEKKLDSLTIEDWFKETPHIFETSFWYMWQTA
                     FALKKGSSLSEFRRRVKRMLLEFGRIDTREGMIGLPLNPYDSLIRPLEVYLRSRGVEF
                     IEDCQVTDMEFASGSGITVKTLYLKRRLQDEDESRESFAFEKAELKQGDFCIMTTGCM
                     TDSFSLGDMDTPAPAPSKKSMSSELWSRIACVKPGMGAPEPFFACPEKNSWMSFTVTA
                     RGDALLKAVEEFSGNAPGSGALMTFKDSGWLISSTVAAQPYFAGQPEDVTVFWGYGLY
                     PEAEGDYVKKPMKDCTGREILKEYLSHLHVNEKRMEELMDTVINVIPCRMPYADAALA
                     PRKYTDRPKVIPAGSGNFAMIGQFVEIPEDTAFTEEYSVRAARMAVYSLMDVRRKVIP
                     VTPYWKHPRVLLKTVLKAFR"
     gene            complement(11497..12372)
                     /gene="rsmA"
                     /locus_tag="ACZPCG_07130"
     CDS             complement(11497..12372)
                     /gene="rsmA"
                     /locus_tag="ACZPCG_07130"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HIR1284875.1"
                     /translation="MDNKLVNPQKTIETIQKYQFAFQKRFGQNFLIDSHVLDKIVNAA
                     GITQDDCVLEIGPGIGTMTQYLAEHAGRVVAVEIDTNLLPILAETLKDYSNVTVINED
                     ILKVDMNQLVKEYNQGRPIKVVANLPYYITTPIIMGLFESNVPIENITVMVQKEVADR
                     MQVGPGSKDYGALSLAVQYYASPYIVANVPPNCFIPRPNVGSAVIRLTRYQEPPVHVK
                     DTALMFRLIRASFNQRRKTLQNGLNNSPELSFSKEEIAAVIEGLGLPAAVRGEALTLE
                     QFAALANAFSDPKGK"
     gene            complement(12377..13150)
                     /locus_tag="ACZPCG_07135"
     CDS             complement(12377..13150)
                     /locus_tag="ACZPCG_07135"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HIR1284876.1"
                     /translation="MIFDTHAHYDDEAFDDDREALLGSLASQGIQAVTNVAASLKSCE
                     TTLALTEQYPFIYGAIGVHPSETAELDACGMDRMKAWCASEKIRAVGEIGLDYYWEEP
                     EHEIQKKWFAAQLDLAKELKLPVIIHSRDAAKDTLDIMKAEHAEELGGVIHCFSYTKE
                     MAREYLNMGFYLGIGGVLTFKNAKKLVEVAEYAPLSSIVLETDCPYLAPVPFRGTRNS
                     SLNLGIVAERLAQIKGTDRETVERITWENAERLYRLKEN"
BASE COUNT         3058 a         3876 c         2746 g         3578 t
ORIGIN      
        1 catgaaaagc tctgcagcca gccccagcag ccggtgatgg cctttgaatc ttatcttgag
       61 gaaaagaaaa tccctgtatg gatccgccat gtagtggttc cgggaatcac tgaccaggag
      121 gaatatcttc taaggcttgg ccaatatctg ggaatgcgtt ccaatattaa ggctctggac
      181 gtactgccct atcacgatat gggaaaggtg aaatacaaaa atctgggaat cccctatccc
      241 cttgaacata ctgctcctct ttcaaaagag gcagctgcaa aagcccgcca gatcatcctg
      301 aaaggaatga gggaaagccg gggactatag gggattgctt tcaaatttgt acaatcccct
      361 gcctttccgg cagcattttc ctcttgaata attaagagtt ttagtataga atataagaac
      421 aatgtataca ttaaataaag gaggtacttt cattatggca cacgtaatta ctgacgcttg
      481 cgtaagctgc ggaacctgtg ctggcgaatg tccagtaaat gcaatctctg agggagacgg
      541 caagtacgtt atcgacgctg acgcttgcat cgactgcgga acttgtgcag ctgcttgccc
      601 aacaggcgct atcgaggctt aattcagcta cataaaagac cactgcatat gccaaaaggc
      661 agacgcgcag tggtcttttt ttgttaagga ggattcttat gacacctgta aaacccatca
      721 tcggtatcac accttcacac aatacagaaa atgacgacac cagcctccga cccacctatc
      781 ccagagcaat ccgggcagcc ggcggtctga cgatccttct tcctctggag ggcaccgacg
      841 aggactggcg ccagctggcc gccatgtgtg acggctttct cttttccggc ggcccggatc
      901 ttcatccctt tttatttggg gatgaaacac attctgcctg cgggaatgta tctcctgtca
      961 gagattccat ggaaatccgc ctccttaaag ctgccatgga gctgaaaaag cccattttag
     1021 gtatctgccg gggcatacag cttttaaatg tggctcttgg cggaaccatt taccaggata
     1081 tcccaagcca gatggagcga acctttcctc tggcccacaa gcagcccttt tactataccg
     1141 taccgtctca tcatgtggat ataaaaaagg gaagcctttt atcaaaggct tccggcggcc
     1201 tggagcgtat ctctgtcaac agccagcatc atcaggccat taaggatgct gctccgggcc
     1261 ttatacctgt aggctgggct ccggatggag tcatcgaggc tgtagaaaag ccggattacc
     1321 ccttttttct tggggtacag tggcatccgg aatatctctg gcagaatgac ccggcggcca
     1381 tagggctgtt ccggcttttt gtagaggcct gcaaagcttc atagatcatt ctgcgtctat
     1441 gatctctcct gccatctggt aaaattcctc cggcgtcatt tccacatcaa agcccagaag
     1501 agtataaaac tgtccgttgt cctcccaggt aagcgtctgg gctgtctggt cgatcacccg
     1561 gtctgtaccg tatgaaataa ccagctctcc ggctgcctcc tgggccttct cttctgcgct
     1621 tggctcataa tccggtggaa ccatacggta atgatcctgg ctgtacttta aggtaatccc
     1681 ctcatgcacg gcggtctggt cgggagcatc ggattcatca aataaattgc cgtgttcgat
     1741 attcaggctc agatccgcca tgttttcctt tgaatacatc agagacagat cctgctgctc
     1801 ctttaccaca tttccctgct catcctctcc cctgctgtaa acaggagtac catatgcaaa
     1861 gcggtatccg tttgaaaatg cctctggcgc ctttgtttta agccccagct gctcttcctt
     1921 ttctgccagc tggctgtatt ccgtgaaatc ctccgcatgg ctggaggagg atatggccgt
     1981 ttttacctgc cctgccgcca ctgcggtcat tgctccaacc aaacatactg ctgctaccaa
     2041 aatagctgtt ttcctcatat tccatgtttt tctcattgaa ccttcctcct ctattctctg
     2101 ataaacgcct ctgcacatcc gttctgtctg ctctggcccg atcgccagcg tctctgcctg
     2161 ttccttcaat gcattccgga gaaacgaatc cattttttca tcttctctgc ctgtcagctg
     2221 cttcataaaa gtgcctccct tcccatggcg cgctcctttg taagcgctgt cttcagattc
     2281 tttctggctg taaacagtcg ggattttaca gttccttcca aacagcccgc cactgctgca
     2341 atctcccgtg tggacattcc attataataa tacaacacta aaacagtacg ctgtttgacc
     2401 ggaagagctg ctatagctgc aaacagttcc tgccttccgg aagcatttac tacatcatcc
     2461 agcacagatc catttccgga ggtatcctca gaaagcacct cctctacagg ctgttctctc
     2521 ctattccttc ttcccacccg ccatgcttct ctggtcatgg ttcgataaag ccatttccct
     2581 atatgctccg gatcccggat ccgcatccgg ttgatccagc aggcagtaaa tgtctcctga
     2641 acaatgtcct cactgtctgc cctgtttcct gcgatcagat acgctgcccg cagaaggccc
     2701 ggataaaaat gtctcatcaa ctcgtcaaat gcctcccggt ctcccgccgc aaccctgcat
     2761 gccaggcttt tggcccactg ctcatccatg gccgttcctc cttgtttctt gttgaatcat
     2821 tttgttgtct ttttgggtgc acctgttata tagaggggaa tgaggtggta taggttcatt
     2881 tttattttca ggtaaaaaat acttaaaagc catatccagt cactttatga tcaagatata
     2941 aagaaagcac ccactccaaa gtggatgctc caaagcaacg gttatatatt cattcttacg
     3001 agcaatgcct cccccactcc ccaaccatct ctctcatctg tctgaccgtt ccttcaatat
     3061 cctctgcccc aaagatcccg gacactacag ccgctccggc tgccccgctg cctttaagct
     3121 ttccaatatt ttccaggcag atccccccga ttgccacaac cgggatggaa acggaccggc
     3181 aaatctctcc caaccgctcc atagtcatag gaagggcgtc ggctttggtg gaggtgccga
     3241 atacggctcc acttcccaga taatctgctc cctgctcctg agctttcagg gcctgctcca
     3301 ctgttttcgc tgtaacgccc agaatcttat ccggcccaag gagcgctctg gcctgtgcgg
     3361 catccatgtc attctgtccc acatggacgc cgtcagcacc gcacagaaga gccagcttta
     3421 tattatcatc aatgatcaga gggaccttgt accgctccgt gagagcccgc aggcttcgag
     3481 cctcctccag ataatcctgc accgtatcat tcgtcaggcc cttttccctc atctgcacca
     3541 tggtagctcc gccctttaac gcagcctcta cctgctcgca caagctcatt ttcccggtcc
     3601 aggcccggtc tgtcactgcg taaaccataa gctgccttct gtcaaatttc atatctgatt
     3661 tccctcattc tctcttaaat cactcatcaa aaatgcttac aatttctccg tctaaaatcc
     3721 ggttcatatt tttcactgca cagaaattgc cacacatgga acaggtatcc tctttttccg
     3781 gctttgcaga cgcccggtaa tttctggcct tttccggatc aatggaaagc tcaaaggtct
     3841 tttcccagtc cagctttttt ctggcaaggc tcatctggcg gtcccagtcc gcagcgcctt
     3901 tgattccctt tgcaatatcc gccgcatggg ctgcgattct ggaagcgatg attccctcct
     3961 ttacatcatt cagatccgga agccgcaggt gctccgccgg cgtcacatag cacaaaaagg
     4021 aagctccgct tgaggctgca atcgccccgc cgatggccgc agtaatatgg tcatatccgg
     4081 gagccacatc cgttaccaga ggacccagca catagaaggg agcgcccttg cagatggtct
     4141 gctggatctt catattggcc tcaatctggt ttaagggcat atggcccggc ccctcaacga
     4201 ttacctgtac atccttttcc caggcccggc gggtcagttc tccaagcgtt accagttctt
     4261 caatctgtga aatgtctgtg gcatcgtcaa tgcatcccgg gcggcaggca tctcccaggc
     4321 tgatggttac atcatgctcc cggcagattt ccagaatccg gtcataatgc tcataaaacg
     4381 gattttcctt tcccgtcatc tccatccagg caaaaataat ggaaccgccc ctggatacaa
     4441 tattcatcag gcgcttattt tccttaaagc gctgtgcggt cctgcggttg attcctacat
     4501 ggatggtcat aaaatccact ccgtcctcag cgtgcatttc cacaatcttc agccattcct
     4561 ctgtggtaat atcctttaat ggcttatgat aataaaccac tgcgtcataa atcggcactg
     4621 ttcctatgat cgccgggcac tctgctgtaa gctttctcct gaacttctgc gtatcgcccc
     4681 aggagcttaa atccataata gattcagcgc ccattccaac tgcatagttt accttttcaa
     4741 gctccatatc cagatccttg cagtctctgg aggttcccaa gttcacattg atcttagtcc
     4801 ttagcataga accgattcca ttaggatcca gacagcgatg attcttattg gcaggaattg
     4861 ccgcctttcc ctctgctacc agagccatga gctcatttac ctcaaaatgc tccttctctg
     4921 ccacctttct cagttcctct gtcacaatgc cctttctggc ggcatccatc tgtgtggtgt
     4981 attccatctt tcttcctcct tgtattctgt gcctgaagca cgttttcgct tcccaaagag
     5041 gctgccggac gggtctgtat gccaaaatct aaaaaacctg cttcccgcag tttctccgga
     5101 tccctgcaat acaaaaggcc cgccccataa ggaacggacc gtgtatacac tcccatgaac
     5161 caaggttcat atccagccga aatatttccc tacgttggca ttacccaaat caggtacgat
     5221 aggtcgaagt tcgattactt cctctcagcc tgctccacaa gctccctgtt gtatctcagc
     5281 atatcactgg aagacgggat atgtcaagcg atttccattt cttttaaaac tccccctgct
     5341 tattccaata ttctcccatc tgtggaaatg gttactaccc gccagggaat aaatctgccg
     5401 cttatctgca aagccttttt tacagcctgc tcaattcctc cgcagcaagg cacctccata
     5461 cggacaaccg tcacgctttt aatgttatta tttgccagaa tctccgtcag cttttcgctg
     5521 taatcccccg catccagctt gggacagccg atcagcgtaa catggtttcg gataaactca
     5581 ttatgaaaat ttccataagc ataagccgta cagtccgctg ccaccagaag gttcgcattc
     5641 tcaaaataag gagcattcac cggagccagc ttaatctgta ccggccctgt gacagctggc
     5701 tgtgaaccga aacaactggc tcactgcatg cattacgttc cctctgaata ctctttgact
     5761 gcattcccgg acagccacag ggaagcggct caaaggatgt ctttttctta gccgccaaaa
     5821 cagccgcctc atcatatgcc ggagcctctc tctcctcaaa cccaatggca tctgccggac
     5881 acgcaggcag gcagtctccc agtccgtcac agtaatcctc acgggtcagc ttcgccttgc
     5941 cgtctatcat ctctatcgca ccttcatggc aggcagccgc acaggctccg cagccattac
     6001 acttctcttc gtcaatctta ataatctttc taatcataat ccgctctcct tttttaattc
     6061 ttccatatgc cctcacagca aacctccctg tctgctaatg ataccgccat tgtaatacat
     6121 ctctaagaat aattccgttg tttctgcaac aaaacacaag ttttcttccc acagcaccaa
     6181 aaaatgcaga acccctgtca catccacagg cattctgcat cctacatttc tcagatccgt
     6241 cctccgcaga ccggacagta attataatcc tcatctgttt caaacccgca tctcatacac
     6301 tttttatgag gctttttaga gccctcctgc caggttttgg cgtaccgtcc cgcctgcacc
     6361 agacgcaggt ctgcctctgc aatatcaggg ttttcccctc tcaaaacagc cttgcccaca
     6421 accggatcca gttcataaac cgcctcacag cagtccatct tcacataata acgccggttc
     6481 catttaaaca gcggaataaa gaaaaagcta aaatacatat acgtcatgat cacctgatac
     6541 cgcccatggc tcccacagct cccgcaaagc cgcacaagcc cctcactcaa tatcttctgt
     6601 ccctggctca cgcctccaac aaatatcacc aattccacct cccacatctc tgtgtcaact
     6661 ctaccatcat acccccatcc ctcactcccg tcaatcccca tacatttcta tcccccaaat
     6721 ctcactcccg ccatcccttc cgccgcctcc cattcaggct atcagggcga ctttggaggc
     6781 cattagaaaa tattagagga tacaattttt cgttaaaagc catatcaaac ctgtctgagc
     6841 gcagcgagtt tttgatatgg cttttgcatt tagaaaaatt gcatcctctt agattttctt
     6901 atggccggaa accggagccc tgagagcctg aacgggaggc ccacacgact cttaaaccat
     6961 ataccccccc ttcataggag acaccgcgtg ttcggaataa atctttaaca cgccctttgt
     7021 atacttcgga gccttgggct tccatgccgc acggcgttct gccagaacct tatctacctc
     7081 ttccttcgtc atctcctgac cgtggattcc cacgatttcc agaacacgct ctggaatatc
     7141 aatgcggatc agatcccctt cctctaccag ggcaatgggg cctccatctg cggcctcagg
     7201 agacacatgg ccgatagccg gtcccttgga agcgccggag aagcgtccgt ctgtaatcag
     7261 agcaatggtc tttccaagct ccggatcaga agaaatggcc tctgtggtat agaacatctc
     7321 aggcataccg gagccctttg gtccctcata gcggataaat accgcatcac ccggccggat
     7381 cttgtgggac agcactgctg caatggcatc ttcctcacag tcgaatggtc ttgcctttaa
     7441 tacagcctta aacatttcct tcggtaccgc agaatgcttt acaacagagc cctccggagc
     7501 caggtttcct cttaaaattg ccaccgttcc gtcggtgccg atgggctcgt caaaggtacg
     7561 gatcacatcg gtgcgcttta agccccattt ctttaaatag ctctcgcact tgtcatagaa
     7621 accgtttttc tttaagtcct ccagattttc gcccagagtc tttcctgtaa cggtcatcac
     7681 atccaggtgg agcatggact tgatctcctc catcactgca ggaacgccgc ctgcatagta
     7741 gaagaactgt gccggccact tgcctgcggg gcggatatcc agaaggtaat gagccccccg
     7801 atgcatccgg tcaaaggtat ctgcatcaat ctcgataccc agctcatggg ccatggccgg
     7861 aatatggagc agggaattgg tggagccgga gatggcagca tgtaccataa tggcattttc
     7921 aaaggccttt tctgttacaa tgtcgctgac cttgattccc ttctttacaa gctccattac
     7981 ctgttttcct gccttgtagg ccatttcctt taagtcctcg caggtagcgg gcatcagggc
     8041 agaacctggg agcatcagac ccagagcctc agccatgatc tgcatggtgg aagcggttcc
     8101 cataaaggag caggctccgc aggaagggca ggcgtggtgc ttataatagg taagcttttc
     8161 atctgtaatc tctcctctct ggcacatggc agaatacata ccgatctgct ccagtgttaa
     8221 gagatcaggg cctgcatcca tcacgcctcc ggtgatcacg atagaaggca tatccaatct
     8281 tgccagtccc atcaggcatg caggtacgga cttgtcgcag ctggcaataa atacgccgcc
     8341 gtcaaagccg gtggaatttc catggatctc gatcatattt gcgatcatat cgcggtgagc
     8401 cagagaatag ttgatgccgt catgtccctg ggcaatgccg tcacagatat ccgttgtata
     8461 atatctggcg cctcttgctc ctgcgtcggt gattcctctc atggcttcct ctacaaactg
     8521 atccagatgg gcgcttcccg gatggctgtc cccgaaagtg ctctctacca ggatctgggg
     8581 cttatccaga tcctccaccg tccatcccat accaatcttt aagggatcat tttctggcgc
     8641 aagtgctctt actctctgac tgttcagttc cttcatgttg gttcctcctt ctttctataa
     8701 caatttcgtt tcataatcat cagatgtttt ctcttttaat catatcattt cataaatata
     8761 attacaagtc atcagactac cattctactt tttgccaaaa actcggatgt ttttttaggt
     8821 atgatacaca aaaagcccag atatttcgct ttatccaggc tcctgtcaaa catataatgt
     8881 aatttttacg gtaaaatcat ctgatatctt accctgttac tccttgtgat ttttttcttt
     8941 ttcctcccgg atcagcttca ggatcatatt ccggttgtag gtcatatgca tcatcatggc
     9001 ggtttttgct ccaatgggat cccgatcgga aatggcgtcc acaatggctc tgtgcgtcat
     9061 aacggtttcc tttgtaagcc gttggtgggt cacatttaca aacatcatta ctgccgtatc
     9121 aataatgggg atcagctgct ccactacctt attaccggag caccgggcca cgctggtatg
     9181 aaaggcaata tcatcctgaa tgtacatatc tcctgaagcg atccgttcct ctacccgttc
     9241 gcagcagcag cgcagctcca aaattgcttc ctctgtagca tttaatgccg ccatttctgc
     9301 aataccaggc tccaggatca gccgcacatt gaccagatcc agcgccagcg ccatcttatc
     9361 ctctgctccg cttaaaccca gaggatccag atcggaaggc gttgtattta ccacataagt
     9421 gccggatccc cggcgcacct ctaaaatccc ctgggaaacc aaaagcttta ccgcttcccg
     9481 gatggtgctg cgccccacgt caaatttttc tcccagctca aactcatttg gaagtttgga
     9541 gcccggttca agaggcgtct ccaaaatata gtgataaatc tgctcctgaa cctgctctgc
     9601 cagaagctta cttttaatat tggaaaatcc tcccattttc atctcctctg ttccctgata
     9661 tccgcagtta ccgaaacgcc ttaagcactg tcttaagcag cactctggga tgcttccagt
     9721 acggtgtcac aggaatcacc ttccggcgca catccatcag gctgtataca gccattctgg
     9781 cggcccgtac cgaatattcc tctgtaaatg ccgtatcctc cggtatttcc acaaactgtc
     9841 cgatcatggc aaaatttccg gaacctgccg gaatgacttt cgggcggtct gtatatttcc
     9901 ttggagcaag agccgcgtct gcataaggca tccggcaggg gatcacattg atcaccgtat
     9961 ccatcagctc ctccatccgc ttttcattta catgaagatg gcttaaatat tccttcagga
    10021 tctcccgtcc cgtgcagtcc ttcatgggct ttttcacgta atccccctct gcctccggat
    10081 acaatccata gccccagaat acagtcacat cttcaggctg acctgcaaaa taaggctgag
    10141 ccgccacggt actgcttata agccatccgg aatccttaaa ggtcataagc gccccgcttc
    10201 cgggagcatt tcctgaaaat tcctcaaccg cctttaaaag cgcatctccc ctggctgtca
    10261 cagtaaagct catccagctg tttttctccg gacaggcaaa aaacggctcc ggtgctccca
    10321 ttcccggctt cacacaggca atccttgacc atagctcgct gctcatggac ttttttgacg
    10381 gagcaggagc cggagtgtcc atatctccca aagaaaagct gtccgtcata cagcccgttg
    10441 tcataatgca gaaatctccc tgtttcagtt ctgctttctc aaaagcaaag gactcccttg
    10501 actcatcctc atcctgaagc ctgcgtttta agtacagcgt tttaaccgta atacctgaac
    10561 cggaagcaaa ctccatatct gtcacctgac agtcctctat aaactccaca cctctggaac
    10621 gcagataaac ttccagagga cggatcaggc tgtcataggg attgagaggc aggccgatca
    10681 tgccctccct ggtgtcaata cggccaaact ccagcagcat cctcttcaca cggcgtctga
    10741 attcagacag gctgctcccc ttttttaaag caaaagcagt ctgccacata taccagaagc
    10801 ttgtctcaaa tatatgaggc gtttccttaa accagtcctc tatggtcagg ctgtccagct
    10861 tcttttcatc tgtcagcaga agccggaaaa gagccatcct gtccccctga tcaaagccca
    10921 tggattttac atttaaaaac gttccctccc ggtctgccag acgtccatgg gtacgtccgg
    10981 aatgagaatg ggcaaactcc tgaatttcct ctgcaacgct gcgccctgtt ttttttaagg
    11041 atggaatggt atcaaacagc tcccagaaat tctcatatgt cttttcattg agcatatggc
    11101 cgccgcggca tacataaccc ttttccggtg ttcccgctcc gtcatttcct ccccccagga
    11161 tatgggttcc ctcatagatg gtaatgtggg agccggggaa attacagtcc cggatcagat
    11221 aagcggctgc agccatggac gccaggccgc cgccgatcag gtacgccctg cggctgccgt
    11281 agtcgcggtt tcgcaccagt gcggcatcaa ttgctgcctg ccgctcctct tcctttctgt
    11341 gccttacctt tttcagttct cttaacgtaa cgagcgccgc tgtacctgca gctgccgccc
    11401 caaggatgcg ggccgtccgt ctgttcatag ccatatcgat cccttccttt cttttacctg
    11461 ctcatccgat cacagaactc cccattttac cctgttttat tttcctttcg ggtctgaaaa
    11521 tgcatttgcc agggctgcaa actgttccag cgtcagagcc tctccccgca ctgctgcagg
    11581 aagccccagt ccttcgatca ctgccgcgat ctcctccttg gaaaatgaca gctccggaga
    11641 gttgtttaat ccgttctgca gtgtctttct tctttgatta aaagacgcac ggatcagacg
    11701 gaacataagc gccgtatcct ttacatgaac cggcggttcc tgatagcggg tcagacgaat
    11761 tactgcagaa cccacatttg gccgggggat aaagcaattt ggaggcacat ttgccacgat
    11821 ataggggctg gcgtagtact gcaccgccag ggacagagcg ccgtaatcct tggagcccgg
    11881 ccctacctgc atccggtctg ctacttcctt ctggaccatt accgtgatat tttcgatcgg
    11941 aacattactt tcaaaaagtc ccatgataat tggcgtagta atatagtagg gaagatttgc
    12001 cactaccttt attggccttc cctgattata ctcctttacc agctgattca tgtctacctt
    12061 aaggatatcc tcattgatca cagtcacatt ggaatagtcc tttaaggtct ctgccaggat
    12121 cggaagcagg ttggtatcaa tttccaccgc taccactcgg cccgcatgct ctgccagata
    12181 ctgggtcatg gttccgattc cgggtccgat ttccagaacg cagtcgtcct gtgtaatacc
    12241 agcggcattt acgatcttat ccagtacatg gctgtcgatc aggaagttct gtccgaaccg
    12301 cttctgaaat gcaaactggt atttctgaat ggtttctatg gtcttttgcg ggtttactaa
    12361 tttattatcc attaattcaa ttctccttca gccggtataa ccgttctgca ttttcccagg
    12421 tgatccgttc cacggtttcc cggtcggttc ccttgatctg tgccagacgc tccgctacaa
    12481 ttcccagatt cagggaagag ttgcgcgttc cacgaaatgg caccggagcc agataggggc
    12541 agtccgtttc cagtacaatg gagcttaaag gcgcatattc tgccacctcc accagctttt
    12601 tagcattttt aaaggtgagg acgccgccga ttcccagata aaagcccata ttcaggtatt
    12661 ctctggccat ttcctttgtg taggaaaagc agtggatcac gcctccaagc tcctctgcat
    12721 gttcggcctt cataatatcc agagtgtctt ttgccgcatc ccggctgtgg atgatcacag
    12781 gaagcttcag ctcctttgcc agatcaagct gggctgcaaa ccatttcttc tgaatctcat
    12841 gctccggttc ctcccagtag taatccagac cgatttctcc tactgcccgg atcttctctg
    12901 aagcgcacca ggccttcatc cggtccattc cgcaggcatc cagctctgct gtttcgctgg
    12961 gatggactcc tatggctcca tagataaagg gatactgctc cgtgagagcc agagtcgttt
    13021 cgcagctttt taagctggca gccacattgg tcactgcctg tatgccctga gatgccagac
    13081 tgcctaaaag tgcctctctg tcatcatcaa atgcctcatc atcataatgg gcgtgtgtat
    13141 caaaaatcat cttgccgtct cctctgttca gttaaaaact gccgcagaac acaaacctcc
    13201 tgacggcggc ctgcatcctg cggcagcagg gacttaatcc ggttctgatc tcacagac
//
LOCUS       DBMDUH010000066        13094 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_2500_length_13094_cov_4.194874, whole genome shotgun sequence.
ACCESSION   DBMDUH010000066 DBMDUH010000000
VERSION     DBMDUH010000066.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 13094)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13094)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13094
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2500_length_13094_cov_4.194874"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            126..1415
                     /locus_tag="ACZPCG_07140"
     CDS             126..1415
                     /locus_tag="ACZPCG_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012739966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family RNA-guided transposase"
                     /protein_id="HIR1284877.1"
                     /translation="MNSTQNKKIDQVKETTMIVGIDVGSEKHYFRAFNWRGIELTRKP
                     VAFSNSMEGFNSFYNTIVELMQKRELEEALVGIEPTGHYWFDLGQFMGEKNIKFVMVN
                     PHHVHKTKELDDNSPSKNDRKDPRVIAGLVRDGRYFYSYMPTGVYAELRNASNRRFVL
                     VEEQTRAKNRLQKWIAVYFPEYKGIYTHIDAKGGLLVLKQAPTPEEIVRLGVEGIIKI
                     WKDAKLRGNGHKKAMLILNAAMKSIGLKSGLTEARMEIQDLIEDYELQTKRLERVNDL
                     IKELCQQVRYADKLLEIKGIGITTVAGFIAEVGDITRFDSTKELQKLAGLELVSDSSG
                     KHNGKTKISKRGRKRLRYLLFEAAISVVGKNEEFKEIHSYYTTREKNPLKKMQSLIAV
                     ACKLIRVFHVILTKGISYDASKMLKDIRHQGEQLKAA"
     gene            1922..2539
                     /locus_tag="ACZPCG_07145"
     CDS             1922..2539
                     /locus_tag="ACZPCG_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284878.1"
                     /translation="MDGTVREYERILTELQDMLFETNEDYKALRKEFETVRSDFDSIE
                     TRERSKQLLLLQKKIKAYSWRIRHVESQLAYIAKVSGPDAARQNLIQTYVVSWSAPPD
                     LAAILTKEELYQEAAKHLKEDIKKLLHNLAECDVKAVPCSKAELFNMLHTHYHPISGN
                     RFHFDDLLESNWDSRIMPYREDTDLEESFTDELIDEMLYGGLQNG"
     gene            2532..4826
                     /locus_tag="ACZPCG_07150"
     CDS             2532..4826
                     /locus_tag="ACZPCG_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284879.1"
                     /translation="MVDFLMMFSLCICVLSGVTLLIVHIWSKKKQKKEEAEDKTGGTV
                     FHSGTRNVPKPIKERAGILQVLAPDGANTMPVSYMTLDDNGIELYYAGLYIDKLPVEG
                     RFAHSFAGIFNMDYVESTVIIDPLLEESQKKINRRIRSLDMERSGERDRNRLRILDGK
                     IADAERWARQIDSDQSTLFETYFLFLIRAESLKLLTERISEFISRGKGIGFRSCYAMH
                     KEAFFTSLPLNEIFEAKISVGKNLSVSKKPIKGHILSQAYLSLIFNHTTSEYFHEKGC
                     VFGRSIYSGMPITFDPFDKTHFSYGAVIAGQSGYGKSATVKQLFSRQVDFGVHIANID
                     YEPLPGDGKRGEYSIVAEAVGGVNYLISNYSDSQLNFFEISDEYEYNRATGEEIPTLY
                     LEEKIVDMTNILMVLATSFTTNGMVGEFEPTEYSRIKSIISKNVRKIYADCGLRDKDA
                     ASLYETVPASGGSFGSGRRKKRLPQMHDFYRAILLDARENTDSFKENAFSLLLDIFED
                     RVREMYYCPHCMKEFTREELSTLKRTEGGVHICNNHEEGKIYYLREIHGSQAYLDCQS
                     TLSIDMSLPFHNFDLSQITDETERINMIMVVQSYIEENFIKKNSTNPNKAKKLIVSTD
                     EAHRILKFEGARMFENALYRVARKRHTAPWLILQSVKDFAKYQDTEEILKSTETFMLF
                     RHNYLDGQYIKDTTNLNQSQVDTVLNLGGTSEAKKYGELCLVDIPTKRAVFIQADYLK
                     DSEFDVVETDVEKIAEHARMKQGA"
     gene            4823..7702
                     /locus_tag="ACZPCG_07155"
     CDS             4823..7702
                     /locus_tag="ACZPCG_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284880.1"
                     /translation="MKQMKHVQIINRKTIRLLLLIFVLTFCMAPVSSYADGPWTDRAD
                     QSIYDSTGDAPDSSTPDMLDGGIFAELLGEFCIGIGDGLNFLLTSFGCDLNAIIFGRV
                     GGASARTGDVALFSFDMGTGNIYGLVGMAMYGYLRNLVFTLMVPVFLYYLVGFLYSNG
                     NPRVKERLLGMSKLFVASSIGFILFPQLLEIALYLRDAMLHAIMTGSQSLILKVADSV
                     GSGVNMGDNSYAFFFGSGGDYNLISQFRAMAKPNGKVVFINSMLYVGAVVYQAYLAVC
                     YISAAVCMVVLVLLFPFAALFEMVERGTISNWMRQVLGILLNPVIDSCLLLIPMFFMI
                     IGTAQESKGYALIALIICGCIIPARGVIRGFLHIGNGMGMELAGFGTIMGATSLMRSI
                     GSGIGAAAGALAHASADAKSDKINADMTDDLADVQKKQDLEDINKGEALQSEMEDIIG
                     ENPDLDMDDAETSFAFGYQPTPKEEMHEKSPHAQAAATAQNIASGIDALGRKKDSLNE
                     HIRSNDSKIASIGEEIAMDNEQIASMKAEKQAIQAEQNEQKKRGDTPSADGYARIREL
                     DQGIGEAEHSRSVRMEEQSRLRGENARTKEEIGRIDALSARARSAISPSRSSFGGGVS
                     GIGNGISEEEQEILDQYATIDNFESPQFKNISLERKAALYRERAAKTRVRGATTAAGT
                     MAGMTFGATAGMAMTAFSSPAAKMQMTAIGGAMGANAGSILGEVMAEPVYEGGVELVR
                     MGGPLLQKGATILKDKAEAYRNNPEGLNVYEEEPQKMLEVNVHSSSKPTVTTVSQSQD
                     NNPSSVETVSGPGSKEQPSYQSDVEAVGKDAGVQSGPQVTYTETVSDEDAAIMQQFYE
                     AEIQKPEFRRTMHHAYTDSAVSARQVYSGLKNSMVNATKEERAVANEQIIETAFMQYT
                     NTILDSLDAPERVDQIAYQQAVEDRLREDERGRMEKYLRSKHLLF"
     gene            7739..8044
                     /locus_tag="ACZPCG_07160"
     CDS             7739..8044
                     /locus_tag="ACZPCG_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284881.1"
                     /translation="MRKQKRLKRKSLETERLKAQHGLQDSTDIIIVEHSNTLTVMIQA
                     AGQIVRFLAATIFVGFCLLGLASVCFPGPRKEMVEIGIALITQLREYLPFLNVIFWR"
     gene            8048..9616
                     /locus_tag="ACZPCG_07165"
     CDS             8048..9616
                     /locus_tag="ACZPCG_07165"
                     /inference="COORDINATES: protein motif:HMM:NF025001.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR1284882.1"
                     /translation="MARPVRTSNLLKEAAADHENLDRTAEQERLPVNRVATQPPVQEN
                     ESVIEKAEEKEPSVQEKLDAILPEDFFGEFEDLFNSADVHTKEQIKQDMPPDSLPEAP
                     ATVTDDGAAQAVLEAAEEKADEEEIAPAINENTAEKMEEEIPAATVEKTEEALSPEPA
                     TAKPEEMPDPLPDTQTQDPKPTVAACRKASVEPAPETREPQNVIRRMPAEEDLTPTHT
                     INRAVRRTYKQIGLSQPVTNVSLQQMISVYSPKGGVGKSTIVRELAHVLSNMKRNGKR
                     LKILVLDADWEFGDVDSAFGICPSPNVMDWVKRICNDRERLGYIPAYSPADIVSRYVI
                     EYDEQIHILAGSDNAMEAARLDCEIAEAIMDNLRRCDYDLILVDNCNTLKDTTVIPLE
                     KSDLILLILSLDTSTVQDARNFLDVLSEFRIDKSNIRMVINQVPLDDKKCELGISHVA
                     RILTMEPTCIIHTNQDARSFANVGEAASSDKGSLFSKEIRTLARAAVAVDEELEKPEK
                     CSGGFLRRLFGRKR"
     gene            9619..11583
                     /locus_tag="ACZPCG_07170"
     CDS             9619..11583
                     /locus_tag="ACZPCG_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284883.1"
                     /translation="MEKELDLLIATEMSGDGDSVAEELRSVFAKRGVTVHRIEFRSGK
                     DSVIRSVRANPQIHAVVLSQYQDQEKLSPRDIDQICSTAEGDLQVFVVVSEMRGSDYM
                     KEIESLGIYTAVYQEDASFEKIAEWYCNGRTKKEARAYYGVAGGDHVVQYRNMDVNAS
                     IQYLLEYDGSYTDLIQRMSVLVNGIPASQVASIVCQLPDPICEMLKREPRFALICQMA
                     EEQYAYLYKPSGQPEKEEAGEKADTGGEEQPKNKAIRRFFKRKQEEPTERKPAKRQAC
                     EIGVMATNIGVGCTTISILLANTLAHEGKRAAIVELDDADGCFEQLCRQEREEEQVDG
                     ITKFAIGTLDYYYHVPLQKFQLDYKPLYDFIVYDFGCLDQETIFHVYARMQNRFIVTS
                     KAEWKQHELKELLKDIKGMPGEDECRVLISSHCGGSDLGDVKELMPENTILAAIPYES
                     NPFYPGKACRQIFLKLLDGTYKEEKYKKSDSVEQRFSERPRDFKKFGIQIVMGALLVA
                     CMGFMVSACSANNRYNRMVTKADTFITGLEEENRQAEDELQKKEKELKALEHEVYYAQ
                     TDIPAGQLISGDMVQKEIVQTEFDDSFFINASDLGSVAASIPIKAGTPLMKAFTGQVF
                     QDPQDGDNDLPDLADLEDIGMEGVESTDGD"
     gene            11654..11971
                     /locus_tag="ACZPCG_07175"
     CDS             11654..11971
                     /locus_tag="ACZPCG_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284884.1"
                     /translation="MNEKLWEKKRKLEDAYDRVMSELEDYSGPTYGGVTETVFALFEH
                     FYRKVRMLALPVSGFSLVIGFLLLLLVRRDKLLFRRIFLTFIVGIPGFFLLFTWGIGI
                     LLN"
     gene            11989..12891
                     /locus_tag="ACZPCG_07180"
     CDS             11989..12891
                     /locus_tag="ACZPCG_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284885.1"
                     /translation="MSENGIGLELLGFLGGTAKSKEAEAQKKLDQYEYLMEKGELLTD
                     IRFTQEIKDKGLQAYDGDVQLIMPTEESTLYKGIFGSTYLLERCYNVKITKLDRETMT
                     VYLSYRAAQAEYRPAALEKIKKSIEAGEELEVKAIVVLCRELQNYIVVDLLGLSIPGV
                     LPFSEWIHGYAANIKEQAVSGKIIDVKIKGYTNKSTEEEPRFLVSRRDCVKSEWVGIE
                     ERFPLHSNIIIECVEMQGKNWIGKIPGVPNISVYCFYPNRLSPTTGGPIIIKIGGQYS
                     CFVAAIDAEKCIFRARVKDMVDRS"
BASE COUNT         3865 a         2557 c         3568 g         3104 t
ORIGIN      
        1 agtaagcttc ctgtataatt caaagtgtag agagtaaatg attttacaaa aatagatacc
       61 taagttaaac tgtcattgct gaccggccag ttagcaaatg agcagaataa gaaaggtatc
      121 tacaaatgaa ttctacacaa aataagaaga ttgatcaagt aaaagaaaca acaatgattg
      181 tcggcattga tgttggcagt gaaaagcatt atttcagagc ttttaactgg agaggcattg
      241 agcttaccag aaagcctgta gcgttttcaa actctatgga gggattcaac agcttttaca
      301 atacgattgt ggaactgatg cagaaaagag aacttgaaga ggcgctggta ggaatagagc
      361 caaccggtca ttactggttt gatcttgggc agttcatggg cgaaaagaac ataaagtttg
      421 tgatggtaaa tccgcaccat gtgcataaga ccaaagaatt agatgacaac agtccatcta
      481 aaaatgacag aaaagatcct cgtgttattg cagggcttgt aagggatgga cggtattttt
      541 attcatatat gccgacaggt gtatatgcag aactgcggaa tgcgtcaaac cgccggtttg
      601 tgcttgtaga agaacagaca agggcgaaga accgtctgca gaagtggatt gcagtgtatt
      661 tcccggaata taagggaatt tatacacaca tagatgccaa gggaggattg ctggtgttaa
      721 aacaggcacc aactcctgag gagatagtaa gacttggagt ggaaggtatc ataaagatat
      781 ggaaagacgc taaactccga ggaaatgggc ataagaaggc tatgttgatt ctgaatgcag
      841 ccatgaagag cataggatta aagtcaggtc ttacagaagc cagaatggag atacaggatc
      901 tgatagagga ttatgaactc cagacaaaac gccttgaacg ggtcaatgac ctgatcaaag
      961 aactgtgcca gcaggtcagg tatgcggata agctgcttga aataaaaggc ataggaataa
     1021 caacagtagc tgggtttata gcagaagtgg gtgatatcac gcgcttcgat agcacgaaag
     1081 aacttcagaa actggcagga ttggaactgg tgtcagatag ctccggtaag cacaacggaa
     1141 agacaaagat aagcaaaaga gggcgcaagc gtctcagata tcttcttttc gaggcagcga
     1201 tatcggtggt gggaaagaat gaagaattca aagaaatcca cagttattat accaccaggg
     1261 aaaagaatcc attaaaaaag atgcagtcgc tgatagcagt agcatgtaag ctgataagag
     1321 tattccatgt gatccttacc aagggaatca gttatgatgc atcaaagatg ttgaaagata
     1381 ttcgacatca gggagagcag ttaaaggctg cataagcgag cactaaagaa caaccataat
     1441 gattacagga agcgtccacc gaaaggtgct gtaatcggag tgtttggatc agtaatgaaa
     1501 gtacaaagaa tgagctggta gccggcagaa attttctcca gagggcatga ccctgtaagt
     1561 aagctaaact ggcgccctgg ttatggacag gcagaacgaa ggaagtgagg acccgccgta
     1621 aggaggatga tcctgttgga catgagaggt gagctgccat atagggatgg gtatacatcg
     1681 aggccattta ggacggatag tgatgacgtt ttatttggtg tacccattat tactatatat
     1741 ctgccctgta tgaggtaaag atcactccat aaccttagtt ctattcaatg acaggtatca
     1801 aaaagtgaat ttattgaaaa aaacacttga ttatatggag agggtcaaaa ataactatgc
     1861 tgcctttata tcggaattta catccagggt tacataccgc atatacgggg aagacatcaa
     1921 tatggatggc actgtccggg aatatgagcg gatcttaacc gaactgcagg acatgctctt
     1981 tgagacaaat gaagactata aagccctccg aaaagaattt gaaacagtaa ggagtgattt
     2041 tgattccata gaaaccaggg agcgttccaa acagctgctc ctgctccaga aaaaaataaa
     2101 agcatacagt tggcgcatcc gtcatgtgga aagtcagttg gcctatatcg cgaaagtgtc
     2161 aggaccggat gctgccaggc agaaccttat tcaaacgtat gtggtttcct ggagcgcgcc
     2221 accggatttg gcagccatcc taaccaagga ggagctgtat caggaagcag cgaaacattt
     2281 gaaggaagat atcaaaaagc tgcttcacaa cctggcggag tgtgacgtaa aagcggtgcc
     2341 ctgcagtaaa gcggagctct ttaatatgct ccatacgcat tatcacccaa tttctggaaa
     2401 tcgtttccat tttgatgatc tgctggagtc caactgggac agccggatca tgccgtaccg
     2461 ggaggatacg gatttagagg aatcctttac ggacgaactg attgatgaaa tgttatacgg
     2521 aggactgcaa aatggttgat tttcttatga tgttcagctt gtgcatctgc gttttgtcgg
     2581 gcgtgacatt attgatcgtg catatctggt ctaagaaaaa gcagaagaaa gaagaggcgg
     2641 aagacaaaac aggaggtact gttttccata gcgggacgag gaacgtgcca aagcccataa
     2701 aagagcgtgc cgggatttta caggtgcttg caccggatgg cgcaaatacc atgccggtat
     2761 cttacatgac actggatgat aacgggattg aactttatta tgccggtctc tatatcgaca
     2821 agcttccagt ggagggacgg tttgcccata gctttgccgg gatcttcaat atggactatg
     2881 tggagtccac ggtcatcata gacccgctct tagaggaatc ccagaaaaag atcaatcgtc
     2941 ggatccggtc actggatatg gaacggtccg gggaacggga cagaaacagg ctcagaattt
     3001 tggatggaaa gattgcggat gcagagcggt gggccagaca gatcgactct gaccagtcaa
     3061 ccctgtttga aacatatttt ctattcctga tccgcgcgga aagcttaaaa ctcttaacag
     3121 agcggatttc agagtttatt tccaggggaa aggggatcgg tttccgttcc tgctatgcga
     3181 tgcacaagga agcttttttt acatccctcc cgctcaatga gatctttgaa gcaaaaatat
     3241 ctgtcgggaa aaatctgagt gtgagtaaaa agccgatcaa gggacatatt ttaagccagg
     3301 cctatttatc cctgattttt aaccatacca caagtgaata tttccatgaa aagggctgcg
     3361 tgtttggccg atctatctac tctggaatgc cgattacgtt tgatccgttt gataagactc
     3421 atttctccta cggagctgtg atcgcgggcc agtccggata tggaaagtcg gcaaccgtga
     3481 agcagctgtt ttccaggcag gtggatttcg gcgtgcatat cgcaaacatt gactatgaac
     3541 cgcttcctgg cgatggaaag agaggtgagt attccatagt ggcggaagcc gttggaggag
     3601 tcaattatct gatttccaat tattccgact cccaacttaa tttttttgag atcagcgatg
     3661 agtatgaata taaccgggca accggagagg agataccaac gctgtatctg gaagaaaaga
     3721 ttgtggacat gacgaatatc ctgatggtac tggcaaccag ttttacgacc aatggcatgg
     3781 tcggtgaatt tgagccgaca gaatattccc gtattaaatc cattatttca aaaaatgtgc
     3841 ggaaaatcta tgctgactgc ggcctacggg acaaggatgc ggcgtctctt tatgaaacag
     3901 taccggcatc cggcggatcc tttgggtcag ggaggagaaa aaagcggctg ccccagatgc
     3961 atgattttta ccgggccata ttactggatg cgcgggagaa tacggacagc ttcaaagaga
     4021 atgcgttctc cctgctgttg gatatttttg aggatcgtgt gcgggaaatg tattactgtc
     4081 cccattgcat gaaggaattt acaagagaag agctgtccac attaaaaaga accgagggag
     4141 gtgtccatat ctgtaataat catgaagaag gcaagatcta ttatctgcgg gaaatccatg
     4201 ggagccaggc atacctggat tgccagtcca cgctttctat tgatatgtcg ctgccattcc
     4261 ataacttcga tttgtcgcag atcacggatg agacggagcg catcaatatg atcatggtcg
     4321 tacagtctta tatcgaggaa aactttatca agaaaaactc taccaatccg aataaggcaa
     4381 agaaactcat tgtgtctacg gatgaggcac accgtatcct gaaatttgag ggagcacgca
     4441 tgtttgaaaa tgctttatac cgcgtggcgc gtaaacgtca tacggctcca tggctgatcc
     4501 ttcagtccgt aaaggatttt gcaaaatacc aggatacaga ggagatctta aagtctacgg
     4561 agacctttat gctgttccgt cataactacc tggatgggca gtacatcaaa gataccacga
     4621 accttaacca gtcccaggtg gatacggtac tcaatctggg cggcacctct gaagcgaaaa
     4681 aatatggtga attgtgtctg gttgatattc cgacaaagcg cgcggtattc atccaggcag
     4741 attatttaaa agacagtgag tttgatgtag tggaaacaga tgtagaaaag atcgcagagc
     4801 acgcaaggat gaaacagggg gcatgaagca gatgaaacat gtgcagatca tcaacagaaa
     4861 aacaatccga cttctgctcc ttatatttgt acttacattc tgcatggcac cggtatcttc
     4921 ttatgcggat gggccgtgga cggaccgggc ggatcagagc atttatgatt ccaccgggga
     4981 cgcacctgat tccagtacac cggacatgct ggacggagga atttttgcgg aactgcttgg
     5041 ggagttctgt attggaattg gtgacgggct gaattttttg ctgacttctt ttgggtgtga
     5101 cttaaacgcg atcatatttg gaagagtggg aggtgcctct gcccgcaccg gggatgtggc
     5161 gttgttttct tttgacatgg ggaccggcaa catatatggc ctggtgggca tggccatgta
     5221 tgggtaccta cggaacctgg tttttacgct tatggtgcca gtattcttat actacctggt
     5281 tggtttcctc tactcaaatg gcaatccgcg ggtcaaggaa cggctgctgg gcatgagcaa
     5341 actatttgtt gccagcagca tcggttttat tctgttcccg cagcttttag aaattgcttt
     5401 atacctgaga gatgccatgc tccatgcgat catgacagga agccagtcgc tgatccttaa
     5461 ggtggctgac agcgtcggat ccggcgtcaa catgggggat aattcctatg cctttttctt
     5521 tggctccgga ggagactaca acctgatcag ccagttccgt gcaatggcaa agcccaatgg
     5581 gaaagtcgta tttatcaatt cgatgttata tgtaggtgcg gtcgtttatc aggcgtacct
     5641 tgcagtctgc tatatttccg cggccgtctg catggtggtt ttggtgctgc tgttcccatt
     5701 tgctgcattg tttgaaatgg tcgagcgggg aacgatatcc aactggatgc ggcaggtgct
     5761 gggaattttg ctaaaccctg tgattgattc ctgcctgctg ctcattccca tgttttttat
     5821 gatcatcggg acggcccagg aaagtaaagg ttatgccctg atcgccctca ttatctgcgg
     5881 ctgtatcata ccggcgcgag gcgtcatccg tggattttta catattggaa atggaatggg
     5941 aatggaactt gccgggtttg gaaccattat gggggcaaca agcctgatgc ggtctatcgg
     6001 aagcggaatt ggagcggcgg ccggtgcgct ggcccatgca tctgcggatg caaaatccga
     6061 taagataaac gcggatatga cggatgacct ggcagatgtc cagaaaaagc aagatctgga
     6121 agatatcaac aaaggtgagg cacttcagag tgaaatggaa gatatcatag gagaaaatcc
     6181 ggatctggat atggatgatg cggaaacgtc ctttgctttt ggctatcagc ccacaccgaa
     6241 agaggagatg catgaaaaaa gcccgcatgc ccaggctgca gcgaccgcac agaatattgc
     6301 cagtggtatt gatgctcttg ggcgcaagaa ggattcttta aatgaacata tccgttccaa
     6361 tgattccaaa atcgcgtcca ttggggaaga aattgccatg gataatgaac agatcgcgtc
     6421 catgaaggcg gaaaaacagg cgatccaggc ggagcagaat gaacagaaaa aacgggggga
     6481 taccccgtcg gcggacggat atgcgaggat ccgggaactg gatcagggaa ttggtgaagc
     6541 ggagcattca cgctccgtac gtatggagga acagtccagg ctccgcggtg agaatgcccg
     6601 gacaaaggaa gaaattggac gtattgatgc tttatcggcc agggcccgga gtgcgatttc
     6661 accatccaga agcagttttg gaggcggtgt gagcggaatt ggaaatggta tctcggaaga
     6721 agagcaggag atactggatc aatatgcgac cattgataat tttgagtctc cgcagtttaa
     6781 gaatatatct ctggaacgga aagcggcgct gtaccgggaa agagccgcaa agaccagggt
     6841 gagaggtgcg acaaccgcgg caggcaccat ggccgggatg acctttggag caacggccgg
     6901 gatggccatg accgcattca gttctccggc agcaaagatg cagatgacgg ccataggcgg
     6961 cgcaatgggt gcaaatgccg gaagcatctt gggcgaagtt atggccgagc cggtatatga
     7021 aggcggtgtc gaacttgtaa gaatgggagg ccctcttctg cagaaaggtg caacgatcct
     7081 gaaagacaag gcagaagcct acagaaacaa tccggaagga ctgaatgttt acgaagaaga
     7141 accgcaaaaa atgttggaag tgaatgtaca ttcctcatca aagccgactg ttacgaccgt
     7201 atcccagtca caggataata atccgtcatc tgtagagacg gtttccgggc cgggatcaaa
     7261 ggaacagccg tcttaccagt cagatgtgga agcagtagga aaagatgcgg gagtacagtc
     7321 tgggccgcag gtgacttata cagaaacggt ttcggatgaa gacgcggcaa tcatgcagca
     7381 attctatgag gcagaaatcc agaagccgga atttcgcaga accatgcacc atgcgtatac
     7441 ggacagcgcg gtgagcgcaa ggcaggttta ttcagggctt aaaaacagca tggtaaatgc
     7501 cacaaaagag gagcgtgcgg ttgcgaatga gcagatcatt gaaacagctt ttatgcagta
     7561 tacgaacacg atactggata gtctggatgc tccggagcgt gtggatcaga tcgcatatca
     7621 gcaggcagtg gaagaccggc tgcgggaaga cgagcgtggc cggatggaaa aatatttaag
     7681 gagtaaacat ttattgtttt agcggaggag tatgaacgta tttacaaaaa ggacacaaat
     7741 gaggaaacag aaacggctga aacgaaaaag tctggagacg gaacggttga aagcgcagca
     7801 tggcctgcag gattcaacgg atatcatcat tgtggagcat tccaatacgc ttactgttat
     7861 gatccaggca gccggtcaga ttgtccggtt tcttgcagcc actatttttg tcgggttctg
     7921 cctgctgggg cttgcgtccg tatgtttccc ggggccgcgt aaagaaatgg tggagatcgg
     7981 gatcgcgctg atcacacagc tgcgggagta tcttccgttt ctgaatgtaa ttttttggag
     8041 gtagattatg gcaagaccag ttcggacttc gaacttatta aaggaagcgg ctgcggatca
     8101 tgaaaatctg gacaggacgg cagaacagga aagattgccg gtcaataggg tagccactca
     8161 accgcctgtt caggaaaatg aatccgtaat agagaaagca gaggaaaaag agccttctgt
     8221 acaggaaaaa ctggatgcga tccttccgga ggactttttt ggtgaatttg aggatctgtt
     8281 taatagtgcg gatgtccata cgaaagagca gataaaacag gatatgccgc cggatagttt
     8341 gccggaggca cctgcaactg tgactgacga tggggctgcg caggcggttt tggaagcagc
     8401 agaagaaaaa gctgatgaag aagaaattgc tccagcaatt aacgaaaata ctgcagaaaa
     8461 aatggaagaa gagattcctg cagcgacagt ggagaaaaca gaggaagcgt taagcccgga
     8521 accggccacg gccaaaccag aagaaatgcc ggaccctttg ccggataccc agacacagga
     8581 ccccaaaccc acagtggctg cctgtagaaa agcttcggta gaacccgctc cagaaacacg
     8641 ggaaccgcag aacgttatca gacgtatgcc ggcggaagag gatctaacgc caactcatac
     8701 gataaacagg gcagtgcggc ggacctataa acagattggt ttaagccagc cggttactaa
     8761 cgtgtcattg cagcagatga tctctgtata ttcccccaaa ggtggcgtag ggaaatctac
     8821 cattgtgagg gaattagccc atgtcctatc gaacatgaag agaaatggga aacggcttaa
     8881 aattttggtg cttgatgcgg actgggaatt tggggatgtg gacagtgcgt ttggtatctg
     8941 cccatcgccc aatgtaatgg actgggtgaa acggatctgt aacgataggg agcggctggg
     9001 atacattcct gcatattcgc cggcggacat cgtatccaga tatgtaatcg aatacgatga
     9061 gcagatccat atcctggccg gaagtgataa tgcaatggaa gcggcgcgtc tggattgtga
     9121 gattgcagag gcgattatgg ataatctgag gcgctgcgat tatgatctga ttctggttga
     9181 caattgcaat acattaaagg atacgaccgt cattcccctg gaaaaatcgg atctgatcct
     9241 gctgatcctt tccctggata cgtccacagt gcaggacgcc cgcaatttcc tggatgtgct
     9301 tagtgaattt cggatagata aatctaatat ccggatggtt ataaaccagg tgccgcttga
     9361 tgataaaaaa tgcgagcttg gaatatccca tgtggccagg atcctaacta tggaaccgac
     9421 ctgtatcata catacaaatc aggatgccag aagttttgcg aatgtcgggg aagcagcatc
     9481 aagtgacaaa gggtccctgt tttcaaaaga aatcagaaca cttgccaggg cagcagtcgc
     9541 ggtagatgaa gaactggaga aaccagaaaa atgttctgga ggatttttgc gcagactatt
     9601 tggcagaaag aggtgagtat ggaaaaggaa ttagaccttc tgatcgcaac ggaaatgagc
     9661 ggggatggcg attcggtagc agaagaactt cggagtgtat ttgcaaaacg cggagtcacg
     9721 gtccacagaa tagaatttcg ctctggaaaa gacagtgtga tccggtctgt ccgggccaat
     9781 ccacaaatac acgcggtggt actcagccag taccaggatc aggaaaaact ttccccgcgc
     9841 gacattgatc agatctgctc tacggcggaa ggtgatttac aggtgttcgt ggtagtttct
     9901 gaaatgcgtg gcagcgacta tatgaaggaa attgaaagcc tggggatata cacggcggtt
     9961 taccaggaag atgccagttt tgaaaaaatc gcagaatggt actgcaacgg ccggacgaaa
    10021 aaagaggccc gggcttatta cggggtcgcc ggaggggacc atgtggtcca gtatcgcaat
    10081 atggacgtga atgccagtat ccagtatctt ttggaatatg acggatcgta cacggacctg
    10141 atccagcgga tgtctgtact ggtaaatgga attccggcca gtcaggtggc atctatcgtc
    10201 tgccagctcc cggatcctat atgtgaaatg ttaaaacggg agccgcgctt tgccctgatc
    10261 tgtcagatgg cggaagaaca gtacgcatac ctttataagc cctccggtca gccagagaaa
    10321 gaggaggcag gtgaaaaagc ggatacggga ggggaagagc agccgaagaa taaagcaata
    10381 cggcgatttt ttaaaagaaa acaggaggaa ccgacagaac gtaaaccggc aaagagacag
    10441 gcatgtgaga tcggggttat ggcgaccaac ataggggttg gatgcacaac catttccatt
    10501 cttcttgcca atacattggc acatgaggga aagcgcgctg cgatcgttga actggatgat
    10561 gcggatggat gctttgagca actctgccgc caggagcggg aggaagagca ggtggatggg
    10621 atcacaaagt ttgcaatcgg aacgctggat tactattacc atgttccact ccagaaattc
    10681 cagctggact ataagccctt atatgatttt attgtctatg attttgggtg tctggaccag
    10741 gaaacgatct ttcatgtgta tgcgaggatg caaaaccgct tcattgtaac atcaaaggca
    10801 gaatggaaac agcatgaatt aaaagaactt ttaaaagata taaagggaat gcctggggaa
    10861 gatgagtgca gggtactgat cagttcccac tgtggcggtt cggacttggg agatgtaaag
    10921 gaacttatgc cggaaaatac gatccttgca gcaattccat atgagagtaa tccattttat
    10981 ccaggaaaag catgcagaca gatcttttta aaacttctgg atggtacata taaggaagaa
    11041 aaatataaaa agagcgattc cgttgagcag cgtttttcag aacggccgcg ggactttaaa
    11101 aaatttggta tccagatagt gatgggagcg ctgttagtgg catgcatggg atttatggta
    11161 tcggcctgca gtgcaaacaa caggtacaac cggatggtta cgaaggcaga tacattcatt
    11221 acaggacttg aggaagaaaa caggcaggcg gaagatgaac tgcaaaagaa agaaaaggaa
    11281 ttaaaggccc tggagcatga ggtatattat gcgcaaaccg acattccggc cggtcagctg
    11341 ataagtgggg acatggttca aaaagagatc gtgcagacag aatttgatga tagcttcttc
    11401 atcaatgctt cggatttggg aagtgtggct gccagcatac cgatcaaggc gggaacgcca
    11461 cttatgaagg catttactgg tcaggtgttc caggatccgc aagatggtga taatgacctg
    11521 cctgaccttg cagatcttga ggatattgga atggaaggag tggagagtac agatggtgat
    11581 taagtcctgg tgcagatact ttacaggaaa atttatcagg gcatggccgg caattacttt
    11641 gcatgctccg gatatgaatg aaaaactctg ggaaaagaaa cggaagctgg aggatgcgta
    11701 tgaccgcgtc atgtcggaac tggaggatta tagtgggccc acttatggag gggttacgga
    11761 aacggtgttt gcgttgtttg aacattttta caggaaggtg cggatgctgg cgttgccggt
    11821 gagtggcttt tctctggtca ttggtttttt gcttctatta ctggtgcgga gggacaagct
    11881 gttgtttcgc aggatttttt tgacatttat tgtgggaatt ccgggatttt ttctcctgtt
    11941 tacctgggga atcggaatat tattaaatta agaagagagg acaaatatat gagtgagaac
    12001 ggaatcggat tagaattatt aggattttta ggaggaaccg cgaaatctaa ggaggcagaa
    12061 gcccagaaaa aactggatca gtatgaatac ctgatggaaa agggagaact tttgacggac
    12121 atccggttta cacaggaaat caaggacaag ggattgcagg catatgacgg ggatgttcag
    12181 ctgatcatgc cgacagagga atccacgctg tataaaggga ttttcggttc aacctatctg
    12241 ctggagcgct gctacaacgt gaagatcacc aaacttgacc gggaaacaat gacggtttac
    12301 ctaagttacc gggccgcgca ggcggaatac cggccggcag ctctggagaa gataaaaaaa
    12361 tccatagagg caggggaaga gttagaagta aaagctatcg tggtcctgtg ccgggaactt
    12421 cagaattaca ttgtggttga tcttctaggg ctttcaatac caggtgtgct tccattttcg
    12481 gaatggatcc atggatatgc ggcaaatatc aaggagcagg cagtttcagg aaaaatcatt
    12541 gatgtgaaga tcaaaggcta cacgaataag tccacagaag aagagccgcg gttccttgtt
    12601 tccagaagag attgtgtaaa atctgaatgg gtcggtattg aggaacgctt cccgctgcat
    12661 agcaacatta tcattgagtg tgtggagatg caggggaaga actggattgg caaaattccg
    12721 ggagttccca atatttctgt atactgtttt tacccgaacc gtctatctcc gacaacaggc
    12781 gggccgatca tcatcaagat tggagggcaa tattcctgct ttgtggcagc cattgatgca
    12841 gagaaatgta tattccgtgc cagggtgaag gatatggtgg atcggagtta aaatagaaca
    12901 aaaattatga aataatgaaa gatatttatg cctgaatagc aatactacat caaaatttgt
    12961 gtataatttt tgaactctta agaaaacggg agttccgttt tcgttgattc attcagaacc
    13021 tcgatccatt gatgaattgt ctgatgctga actcgataca aaattacagc atagctatgc
    13081 acagtcactt gctg
//
LOCUS       DBMDUH010000067        12978 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_2525_length_12978_cov_5.976159, whole genome shotgun sequence.
ACCESSION   DBMDUH010000067 DBMDUH010000000
VERSION     DBMDUH010000067.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 12978)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12978)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12978
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2525_length_12978_cov_5.976159"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="ACZPCG_07185"
     CDS             complement(<1..207)
                     /locus_tag="ACZPCG_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013976385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HIR1284886.1"
                     /translation="MVTGAIKNKVDKIWDDIAAGGITNPITVIEQLTYLMFIRSLDEK
                     ELENEEFANMMGESGDYIFPQTESG"
     gene            complement(376..4152)
                     /locus_tag="ACZPCG_07190"
     CDS             complement(376..4152)
                     /locus_tag="ACZPCG_07190"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HIR1284887.1"
                     /translation="MSVRRIFVEKKPDYAVKAGELREELESYLDIKGVKNVRVLIRYD
                     IENLSEETYKKALVTIFSEPPVDEVYEEEFPKNPGDLVFSVEYLPGQFDQRADSAEQC
                     VKLLKEDEEPVIRTATTYVITAPLTQAQEEAIRHFCINPVDSRQAEETKPKTLVTEFE
                     VPEDIKSFDGFAHASKEDLKELYGTLNLAMTFKDFLHIQKYYAEEEHRDPTVTEIRVL
                     DTYWSDHCRHTTFSTELKNVTFTEGEYRKPIEDTYHQYLADREVIYKGRDDKYVCLMD
                     LALMAMKKLRSEGKLEDMEISDEINACSIVVPVEIDGVTEEWLVNFKNETHNHPTEIE
                     PFGGAATCLGGAIRDPLSGRTYVYQAMRVTGAADPTRPLNETLKGKLPQRKIVNSAAQ
                     GYSSYGNQIGLATGYVKELYHPGYVAKRMEIGAVMGAAPRKYVIRENSDPGDIIILLG
                     GRTGRDGIGGATGSSKVHTTASIEVCGAEVQKGNAPTERKIQRMFRRPEVSSMIKKCN
                     DFGAGGVSVAIGELAAGLQIDLDRVPKKYAGLDGTEIAISESQERMAVVVDPKDVEQF
                     LAYAAEENLEAVTVATVTESPRLVMNWRGKTIVDISRAFLDTNGAHQEADVTLEVPGR
                     EGSLFERREVGDVKKEWLTMLSGLNVCSQKGLVERFDGSIGAGSVFMPYGGKYQLTET
                     QAMVAKLPVLKGHTDTVTMMSYGFDPYLSSWSPYHGAVYAVVSSVAKIVAAGGDYRKI
                     RFTFQEYFRRMSEEPSRWSQPFAALLGAYSAQMGFGLPSIGGKDSMSGTFDDETGEIN
                     VPPTLVSFAVDVNSSKNIITPEFKKAGSRIVVFKAPRDQYDLPDYGQIMEGYHKIFED
                     IKAGKILSAYAVEGHGAAEAVSKMAFGNKLGVKIEHNVDPRDFFAAAWGDIVCEVAEG
                     KMGELSIPYTVIGEVTDQGVFEYGNVHISMDEALKAWTGTLEAVFPTVTGKEKTGEEA
                     RVEEKLYHTDAVYICDHKIGQPTVFIPVFPGTNCEYDSTKAFERAGAKVVTRVFKNLS
                     PEDIRQSVEEYKKEIAKAQIVMFPGGFSAGDEPEGSAKFFATVFRNAVIKEEVEKLLN
                     ERDGLMLGICNGFQALIKLGLVPEGTITEQKADSPTLAMNTIGRHISKMVYTKVVTNK
                     SPWLAGAELGGVYCNPASHGEGRFVANDAWLHRLIANGQVATQYVDDKGNVTMDEYWN
                     PNGSYMAIEGITSPDGRILGKMAHSERRGEAVAVNIYGEQDMKIFESGVKYFKI"
     gene            4463..5941
                     /locus_tag="ACZPCG_07195"
     CDS             4463..5941
                     /locus_tag="ACZPCG_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008725146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HIR1284888.1"
                     /translation="MKIGFDNDKYLSMQSEHIRERIGQFGDKLYLEFGGKLFDDYHAS
                     RVLPGFAPDSKLRMLLQLADQAEIVIVISASDIEKNKVRQDLGITYDVDVLRLIQEFQ
                     DKGLYVGSVVINLYQGQASADIFKAKLEHMGIQVYRHYCIEGYPSNIPLIVSDQGYGK
                     NDYIETSRPLVIVTAPGPGSGKMATCLSQLYHENKRGVKAGYAKFETFPIWNLPLKHP
                     VNLAYEAATADLNDVNMIDPFHLEAYGETTVNYNRDVDIFPVLNAIFEGIYGSSPYKS
                     PTDMGVNMAGFCIFDDEACREASNQEIIRRYYQAINHLAKDEGNSEEVYKLNLLMKQA
                     KIKPEDRKVVTPCLERAQALNAPAAAIELEDGTIVTGKTSSLLGASAAVLLNAIKVLG
                     EIPHDTHLIAPSAIEPIQTLKTTYLGSKNPRLHTDEVLIALSMSAATDPVAKKALGQL
                     PKLQGCQVHTSVMLSNVDIKTFKKLGMNLTSEPVYEHKKLYH"
     gene            complement(6027..7274)
                     /locus_tag="ACZPCG_07200"
     CDS             complement(6027..7274)
                     /locus_tag="ACZPCG_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR1284889.1"
                     /translation="MNEVRGNVIVGQSGGPTAVINSSLAGVYKTARDRGAKKVYGMLH
                     GIQGLLQERYVDLSEHIKNDLDIELLKRTPSAYLGSCRFKLPEISEDRAIYDRIFEIL
                     DGLEVEYFFYIGGNDSMDTIKKLSDYALLNGSKIRFMGVPKTIDNDLAATDHTPGYGS
                     AAKYIGSITKEVIRDGLVYDQQNVTILEIMGRNAGWLTGAAALAKCEDCEGPDMIFLP
                     EVTFDVDEFMHKVAELHKKKKSVVVAVSEGVKTADGRYVCELTDGIEFVDAFGHKQLT
                     GTARFLAEKINREVGCKTRAIEFNSLQRCASHIVSRVDITEAYQVGGAAVKEAFEGET
                     GKMIILKRVSDDPYICVTDIYDVHKVANVEKKVPREWINEAGDYVTDEFVNYIRPLIQ
                     AELTPIMTDGLPRHLYYTDVEKK"
     gene            complement(7468..8943)
                     /locus_tag="ACZPCG_07205"
     CDS             complement(7468..8943)
                     /locus_tag="ACZPCG_07205"
                     /inference="COORDINATES: protein motif:HMM:NF025979.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4454 domain-containing protein"
                     /protein_id="HIR1284890.1"
                     /translation="MKKPVFRAELAAMALIFVGLSAGCAGQRPGRETEMEYSSAPDME
                     QGKMDEIVEMDDAGREESAHGELPLNDSASVRVLEDLGAEASLLSEPFYLAGDSGISC
                     MVYIDPDEGESFMDSHRIQLFFSDDRENASAVAEFVLEPGKDKYEMVINVRPYADGVL
                     ERETEEPACAAFITDEQAGTRIEVESEGAYGGTYYSSCNYEEIGLSERFYTRTELACL
                     PSENLSLLRNTIYAHHGRKFKTDSLNEYFQQKIWYRGRIEPEAFAEELLTDTERANVQ
                     LIRELENIPFEERVKEGYRGPEGISEAPYLPFLLNQREGESSPLTGTHVSRDTGISFD
                     MAQAEDRGTYFTAPGVISCPVTVTQEQMKAVQEGGQEEVTVDELTGKTKILEMENQIL
                     VFHEKGETAADPGWNPYIGIDYDYERGVYKLWQDSDDTIMKPVYKGEIRIAKGAVCGG
                     HVSLLLASAQPRELSLSEEEWGNWILYDSKGTILAVYYLGD"
     gene            complement(9146..9751)
                     /locus_tag="ACZPCG_07210"
     CDS             complement(9146..9751)
                     /locus_tag="ACZPCG_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR1284891.1"
                     /translation="MKLYLIRHGQTDWNIERRIQGQQDIPLNETGRAQAACLTKAMKD
                     KKLTAIYTSPQIRAYATAQAAAGENPVPIIPLPGLMEINYGDWEGKTAQELLERDGDR
                     YKAWWDRPAEVAPPGGETVIQVDDRCLKAWEFIKSQITGDTAIVSHGGLLAHFMEQLL
                     KGSGEEGGERVVHNASITTFEYAPETGRIVLVDFDRCDHLD"
     gene            complement(9761..10411)
                     /locus_tag="ACZPCG_07215"
     CDS             complement(9761..10411)
                     /locus_tag="ACZPCG_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 domain-containing protein"
                     /protein_id="HIR1284892.1"
                     /translation="MAENFYEMYLEEMELIRPLDQEEEKRVLAAAARADKEAGNRLVE
                     GNLARALAVAKEYEGKELPISDLVQEANMALMMAVTEYDGSQEWPDFAETKIRQAVEA
                     ALLEQRSEAEIEETMAARVNVLQTVSQVMAKELGREATVEELAEKMKMTPDEIKDIMK
                     LALDALTVNGEGTAASDGEAEEDTEPAPALRRDGYGRSGRPSLGYDPMRDGWDLNS"
     gene            10542..11417
                     /locus_tag="ACZPCG_07220"
     CDS             10542..11417
                     /locus_tag="ACZPCG_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1284893.1"
                     /translation="MDINYELYKVFYHVAATLSFSEASKQLFISQSAVSQSIKVLEKK
                     LNQPLFIRSTKRVQLTPEGEILLKHIEPAMNLIRKGENQLLEAHTLNGGQLRIGASDT
                     ICRYYLVTYLNRFHKEFPNIHIKVTNHTSIECAKVLENGQCDFIVANYPNSALSGSLH
                     TRVINEFHDVFVANRQAFDLEGKSVSLNQLLDYPIMMLDRKSTTSEFLHQIFQKNRLD
                     LVPEIELSSNDLLIDLARIGLGIAFVPDFCIPEQDKDLFILKLSETLPARQMVVAYNE
                     NLPVSQAAKHFMEML"
     gene            complement(11428..>12978)
                     /locus_tag="ACZPCG_07225"
     CDS             complement(11428..>12978)
                     /locus_tag="ACZPCG_07225"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015574011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HIR1284894.1"
                     /translation="CPRAKGHFDRVNDAVYEIIHEEEGITREKVLRYAERFQVCPFEF
                     CLDISSWTDGIICDYNYVFDPNVRLKRYFAEGEQTGSYLFLVDEAHNLVPRAREMYSA
                     AVVKEDLLLAKRILKGLPGAAKTVSLLDACNKRMLELKRAYGTGKEEEGISHRRVLGA
                     DYELLGDVKLLALELMAMFGELEAFMNENGEFTDRDLVLELYFKVRDFLNIHDRLDDR
                     YRIYARLLEDGSFMVKLMCMDPSECLRRCLDQSESTVFFSATLLPVQYYKELLSGDPQ
                     EYAIYAKSPFSPENRLVLAASDVSSRYSRRGRSQYERIADYLEVIIRERKGNYMVFFP
                     SYQFQEQVEAILLERERTGSLGFDRICQSNRMTEEEREAFLEQFEAKRSRSFAALCVM
                     GGIFSEGIDLKEERLIGAVIVGTGLPQVNTEQEILKEYFDEREGNGFDYAYQYPGMNK
                     VMQAAGRVIRTVNDKGVIALLDDRFLRPEYVALFPREWGTYTVVNRGNVSQALREFWE
                     KFPRQEEM"
BASE COUNT         3149 a         3699 c         2622 g         3508 t
ORIGIN      
        1 ccctgactct gtctgtggaa aaatataatc tccagactct cccatcatat tggcaaattc
       61 ctcattttcc aactcttttt catccagaga tcgaataaac atcaaatatg taagctgttc
      121 aattacagta atcggatttg taattcctcc tgctgctata tcatcccaga ttttatctac
      181 tttatttttt attgctcctg ttaccattcc ttttctccat tctgcttttt atctcgctta
      241 tagcatacaa ttataccatg ccctaaccgc aaaatccata tatacccacg caaaaagaca
      301 gccccccagg attccaaaaa ccactcccag gtgccgtctt ttcactcaaa ttctatcttc
      361 ccttatccat ctctatcaga tcttaaaata cttcacacca gactcaaaga tcttcatatc
      421 ctgttcgccg tagatattga ccgctactgc ctcgccccgt ctctcagagt gggccatctt
      481 gccgaggata cggccgtccg ggctggtgat gccctcgatt gccatgtagg aaccgtttgg
      541 attccagtat tcgtccatgg ttacatttcc cttgtcgtcc acatactggg tggcaacctg
      601 cccgtttgcg atcaggcggt gaagccatgc gtcatttgct acaaaacgtc cctcgccgtg
      661 ggaagcagga ttgcagtaca cgccgccaag ttcggctcct gccagccatg gggatttgtt
      721 ggtaactacc tttgtgtaaa ccatcttgga aatgtggcgt ccgatggtat tcatagccag
      781 cgtaggagaa tctgccttct gctccgtgat ggttccctcc ggtacaagac ccagcttaat
      841 gagcgcctgg aagccgttgc agatacccag catcaggccg tccctctcgt ttaaaagctt
      901 ctcaacctct tccttgatta ctgcattgcg gaatacggtc gcaaagaatt tagcagagcc
      961 ctccggttca tcacctgcgg aaaatccgcc agggaacatg acgatctgag cctttgcaat
     1021 ttccttctta tactcctcta cagactgacg gatatcctcc gggctcaggt tcttgaatac
     1081 tcttgtcaca acctttgcac cggcccgttc aaaggccttt gtgctgtcat attcgcagtt
     1141 ggtgcctgga aatacgggga taaatacggt tggctgtccg atcttgtggt cacagatata
     1201 cactgcgtct gtatggtaga gcttttcttc tactctggcc tcttcgcctg tcttttcctt
     1261 tcctgttacc gtcgggaata ccgcctctaa cgtacctgtc caggccttta atgcctcgtc
     1321 catggaaata tgtacgtttc cgtactcaaa tacgccctgg tcagtcactt caccgatcac
     1381 ggtatatgga atggacagct ctcccatctt gccctctgct acctcgcaga caatatcgcc
     1441 ccatgcagcg gcaaagaaat ctcttgggtc tacattgtgc tcgatcttta cgccaagttt
     1501 atttccaaag gccatcttgc tcactgcctc agcggctcca tggccctcca ctgcgtaggc
     1561 agacagaatt tttccagcct taatgtcctc aaaaatcttg tggtaacctt ccatgatctg
     1621 accgtaatcc ggcagatcat actggtctct tggcgcctta aataccacaa tccggcttcc
     1681 ggccttctta aactccggtg taatgatatt tttgctgctg tttacatcga ctgcaaagga
     1741 aaccagcgta ggcggaacat tgatttcacc tgtttcatcg tcaaaggttc ctgacatact
     1801 gtcctttcct ccgatggaag gaagtccaaa gcccatctgt gcgctgtatg cgccaaggag
     1861 ggccgcaaaa ggctgactcc atctggatgg ttcctcactc atccggcgga aatactcctg
     1921 gaaggtaaag cggatctttc tgtaatcgcc gcctgctgcc acgatctttg caacagagga
     1981 cacaactgca tatacggctc cgtgataggg gctccagctg gacaggtagg ggtcaaagcc
     2041 gtagctcatc atggttaccg tatccgtatg tccctttaat accggaagct tggctaccat
     2101 ggcctgagtt tcggtaagct gatatttacc tccataaggc ataaatacag atccagcacc
     2161 gatggaaccg tcaaaccgct ctaccaggcc cttctgagag catacattca ggccggaaag
     2221 catcgtaagc cattccttct taacatctcc tacctctctg cgctcaaaga ggcttccctc
     2281 acgacccgga acctccaggg taacatctgc ctcctgatgt gctccatttg tatcaaggaa
     2341 ggcacggctg atatctacaa tggtttttcc acgccagttc atcacaagtc ttggactttc
     2401 cgttactgta gctacggtta cagcctccag attttcctct gccgcatatg ccagaaactg
     2461 ctccacatcc ttcggatcta ccactactgc catacgctcc tgggactcgg aaatggcaat
     2521 ctctgtgccg tccaggccgg catatttctt aggaacacga tccagatcaa tctgaagacc
     2581 cgctgccagc tcaccgatgg caacggatac gccgccagct ccgaaatcat tgcatttctt
     2641 gatcatggag ctgacctccg ggcggcggaa cattctctgg atcttccgct ctgtgggagc
     2701 atttcccttc tgtacctcag cgccgcagac ctcgatggag gcggttgtgt gaaccttgga
     2761 ggaacccgtt gcaccgccga tgccgtcacg gccggttctt cctcctaaca ggataataat
     2821 atctcccgga tcagaatttt cgcggatcac atattttctg ggggcagcgc ccataacagc
     2881 gccgatctcc atacgctttg ccacatagcc tggatggtac aattccttta cataaccggt
     2941 tgcaagtccg atctgattgc cgtaggagga atatccctgg gccgcgctgt ttacgatttt
     3001 tctctgaggc agttttccct ttaaggtctc atttaatggc ctggtgggat cggctgctcc
     3061 ggttacgcgc atggcctgat atacataggt acgtccagac agcggatcgc ggattgctcc
     3121 tcccagacag gtagcagcgc cgccaaaggg ttcgatttct gtggggtggt tgtgagtttc
     3181 atttttaaaa ttcaccagcc actcttccgt tacgccatcg atttcaacgg gaaccacgat
     3241 gctgcaggca ttaatctcgt cggagatctc catatcctcc agctttccct cgcttcttaa
     3301 cttcttcatg gccatcagcg caagatccat caggcagaca tacttgtcat ctcttccttt
     3361 ataaattacc tctctgtctg ccagatactg atgatacgta tcctcgattg gttttctata
     3421 ctctccctct gtaaaggtca catttttaag ctctgtagaa aatgtggtat gacggcagtg
     3481 gtcggaccag taggtgtcca gcacacggat ctctgtcacg gtaggatctc tgtgctcttc
     3541 ctctgcgtaa tacttctgga tatgaagaaa atccttaaat gtcatggcca gattcagtgt
     3601 accgtaaagt tccttcaaat cctctttgga tgcatgggca aagccatcaa agctcttaat
     3661 atcctccggc acctcaaatt cagtcaccag ggtcttcggc ttggtttcct cagcctgacg
     3721 gctgtccacc ggattgatac agaaatggcg gattgcctcc tcctgtgcct gggtcaaagg
     3781 cgcagtgatc acataagttg tagcagtgcg gatcaccggc tcctcatctt cctttagcag
     3841 ctttacacac tgctctgcgg aatcggctct ctgatcgaac tgacccggca ggtattctac
     3901 ggaaaatacg agatcgccgg gatttttcgg aaactcctcc tcgtatacct catctaccgg
     3961 aggctctgaa aaaatcgtca caagagcctt tttataggtt tcctctgaca gattttcaat
     4021 atcatagcgg atcagaaccc gcacattttt tacccctttt atatccagat agctttccag
     4081 ttcttctctc agctcaccag cctttaccgc atagtctggc ttcttctcta cgaaaattcg
     4141 tcttacgctc atttaaaaac ctcctgtggt tcgccatacc tgcactctgt aaaatcagaa
     4201 tacagaatca tacattacca ttgatctgcc tatatcataa cacaggtggt ataaattagt
     4261 aaagttaata tatttaatta ctgttatttt ttatacttat aaatattccg ctgctttcca
     4321 acatatcaga agtcttcgtg ccgaatttcc atttttaaaa acacactctg gcacactagc
     4381 ccgaactatg ctatactagg aaacagttga tccgctgtct gctgcggatt ataaagaata
     4441 aaatattggg agaacgaaag acatgaagat cggatttgac aatgataagt acctttccat
     4501 gcagtcggaa cacatcagag aacggatcgg ccagtttggt gataagcttt atctggaatt
     4561 tggaggaaag ctgtttgatg actaccacgc ctccagggtt cttcccggct tcgcacctga
     4621 cagcaagctt cggatgctcc ttcagctggc cgaccaggct gagatcgtca ttgtgatcag
     4681 cgcctctgat atagagaaaa ataaggtacg ccaggatctg gggatcacct atgatgtgga
     4741 tgtactgcgc ctgattcagg agtttcagga taaaggcctt tatgtgggaa gcgttgtgat
     4801 caacctgtat cagggccagg cgagcgcaga tatctttaag gccaagctgg agcatatggg
     4861 aatccaggta taccgccatt actgcatcga gggctatccc agcaatatcc ctctgatcgt
     4921 cagcgaccag ggctatggaa aaaatgatta tatcgaaacc agccgtcctc tggtcattgt
     4981 gactgctccc ggaccgggca gcggcaagat ggctacctgc ctgtcccagc tttatcatga
     5041 aaataagcgg ggcgtaaagg ccggatatgc caaatttgag accttcccca tctggaatct
     5101 ccccttaaag catccggtga atctggctta tgaagcagct accgctgatt tgaatgatgt
     5161 aaatatgatc gatcccttcc atctggaggc ttatggagaa acaaccgtca actacaaccg
     5221 tgatgttgat attttcccgg tactgaatgc aatctttgaa ggcatctacg gaagcagccc
     5281 ctataaatcc cccaccgata tgggtgtaaa tatggctggc ttctgcatct ttgacgatga
     5341 agcctgcaga gaagcatcca accaggagat catccgccgc tattatcagg ccatcaacca
     5401 tctggccaag gatgagggaa attccgagga agtctacaag ctgaacctcc ttatgaagca
     5461 ggccaaaata aagccggagg atcgaaaggt ggtaacgccc tgtctggaac gtgcccaggc
     5521 attaaatgct cctgcagcag ccattgagct ggaagacgga accattgtca caggaaaaac
     5581 cagcagcctg ttaggcgctt ccgccgcagt cctgttaaat gccatcaagg tactgggaga
     5641 aatcccccat gatacccacc tgatcgcacc ctctgccatt gagcccattc agaccttaaa
     5701 gactacctat ctcggaagca aaaatccccg tctccatact gacgaggttc tcattgccct
     5761 gtccatgagc gctgccacgg atccggttgc aaaaaaagcc ctgggacagc ttcccaagct
     5821 tcagggctgc caggtacaca cctccgtcat gctctccaat gtggatatca aaacctttaa
     5881 aaaacttggc atgaatctga cctccgaacc ggtatacgag cacaagaagc tgtatcacta
     5941 agagaaacag atccattctg aacaaactaa atgccggaca gcaggatttt ccagttcccg
     6001 ctgtccggca tttttaccgt ttttttctat tttttctcca catctgtata atacaggtgt
     6061 cttggaagcc catctgtcat aataggcgta agctccgcct ggatcagcgg gcggatataa
     6121 ttgacaaact catctgtcac ataatcgccg gcctcattga tccactccct cggcaccttc
     6181 ttttctacat ttgccacctt atgtacgtca taaatgtctg tgacacaaat ataaggatca
     6241 tctgacactc tctttaaaat gatcatcttg cccgtttcac cctcaaaggc ttccttaacg
     6301 gcagctccgc ctacctgata tgcctctgtg atatctaccc gggatactat atgggaagcg
     6361 catctttgga gagagttaaa ctcaatggct cttgtcttgc atcccacctc acggttgatc
     6421 ttctctgcca ggaaacgggc cgttcctgta agctgcttat ggccgaaggc gtctacaaat
     6481 tcaattccgt ccgtaagctc acagacataa cggccgtctg ctgtctttac cccctcagat
     6541 acagctacca caaccgactt tttcttcttg tgaagctctg ctaccttgtg cataaattca
     6601 tctacatcaa aggtaacctc cggaaggaag atcatatccg ggccttcaca gtcctcgcac
     6661 tttgcaaggg cagctgcgcc ggtgagccag cctgcattgc gtcccatgat ctctaaaatc
     6721 gttacattct gctggtcata taccaggccg tcacggatca cttcctttgt aatggaaccg
     6781 atatactttg cagcgcttcc gtagcccgga gtatgatccg tagccgcaag gtcattgtca
     6841 atggtctttg gaacacccat aaaccggatc ttgcttccat ttaaaagagc atagtcagaa
     6901 agcttcttta tggtatccat ggaatcattt ccgccgatgt aaaagaaata ttccacttcc
     6961 aggccatcca gaatttcaaa aatcctgtca taaatcgctc tgtcctctga aatctccgga
     7021 agcttgaagc ggcaggagcc caaataggcg gaaggcgtac gctttaacag ctcaatgtcc
     7081 agatcatttt taatatgctc cgacagatct acatatctct cctgcaaaag tccctgaatc
     7141 ccatggagca tgccgtaaac ctttttagcc cctctgtccc tggcagtctt ataaacacca
     7201 gccagactgg aattgattac tgcagttggc cctccggact ggcctacgat cacattgcct
     7261 cttacctcat tcataatgga tacctcctaa agaatcttcc tcactcagaa accagccgtt
     7321 ttccggcctt ctcattttgt aagcgcttac aatcatattg ctattttaat accaatattt
     7381 gtaattttca agatagaaac aggcattttt ttacaaacat ctcgtctttt ttttaacata
     7441 atagagtcat ttcgacaaat acttccatta atctcccaaa taatatacag ccaatatggt
     7501 tcctttgctg tcatagagaa tccagtttcc ccattcttct tcagacaggc tcagctctct
     7561 gggctgagcg gatgccaaaa gcagcgacac atggccgccg cagacagcgc ctttggcaat
     7621 ccgtatctct cctttataaa ccggcttcat aatcgtatca tcgctgtcct gccacaactt
     7681 ataaacacct ctttcataat catagtcaat gcctatatag ggattccagc ctgggtctgc
     7741 agctgtttcc cctttttcat gaaatacaag aatttgattt tccatctcca atatctttgt
     7801 cttaccagtg agttcatcca ccgttacctc ttcctgccct ccctcctgca ccgctttcat
     7861 ctgttcctgc gtaacagtta caggacagga aataactccg ggcgctgtaa aatacgttcc
     7921 ccgatcttcc gcctgcgcca tatcaaagga aatgcctgta tctctggaaa catgggttcc
     7981 tgtgagaggt gatgactcac cttccctctg atttaaaaga aagggaagat agggtgcctc
     8041 tgatataccc tcagggcctc tatagccctc ctttacccgc tcctcaaaag gaatattctc
     8101 cagttcccgg atcagctgca cattggcccg ctctgtgtct gtaagcagct cctctgcaaa
     8161 tgcttccggc tctatcctgc ccctgtacca gattttctgc tggaaatatt cattcaggct
     8221 atctgttttg aattttctcc catggtgtgc gtaaatggtg tttcgtaaaa gagaaagatt
     8281 ctctgaaggc agacatgcaa gttctgtacg cgtataaaac cgctctgaca gtcctatttc
     8341 ctcataattg caggaagaat aataagtacc tccatatgct ccctcacttt ctacttcaat
     8401 ccgcgtacct gcctgttcat ctgttataaa agcagcacag gccggctcct ctgtttcccg
     8461 ttccagtacc ccgtctgcat acggacggac attgatgacc atttcgtatt tgtcctttcc
     8521 cggctccagc acaaactctg ccacagccga agcattctct ctgtcatctg aaaaaaagag
     8581 ctgaatccta tgggaatcca taaagctctc cccttcatcc gggtcgatat aaaccatgca
     8641 gctgattccg ctgtccccgg ccagataaaa tggttcggaa agcaggctgg cctctgctcc
     8701 cagatcctcc agtacgcgga cggaagccga atcattcagg ggcagctcac catgagcaga
     8761 ctcctccctg cccgcatcat ccatttccac tatttcgtcc attttcccct gttccatatc
     8821 aggagcagag ctatattcca tttctgtttc ccgtcccggc ctctggcctg cacatcctgc
     8881 cgataatccg acaaaaatca aagccatcgc tgccagctct gctctaaaaa ccggcttttt
     8941 cataaaacac actccctcct gacaatacct tttttatttt tattatactc cctttttccg
     9001 gagcctgttt tacgttttcc ttacaatctg tcccacccgg aaatcccttt cgcgctacat
     9061 agtttccgcc tcagctgcac catgtcaaaa gctccatgct gcagacacag cacagagctt
     9121 ttctactgaa acaatataaa atgttttaat ccaaatgatc gcaccggtca aaatcgacca
     9181 aaactattct tcccgtctcc ggggcatact caaaggtagt aatgcttgca ttatgcacaa
     9241 cccgctcccc gccttcttcg ccgctcccct ttaaaagctg ctccataaaa tgggccagca
     9301 gccctccatg ggatacaatg gccgtatcgc cggttatctg gcttttaatg aactcccagg
     9361 ctttcaggca gcggtcatct acctgtatca cggtttcccc tccgggggga gccacctctg
     9421 ccgggcggtc ccaccaggct ttatagcgat ccccgtctcg ttcaagcagc tcctgtgctg
     9481 tcttcccttc ccagtctccg tagttaatct ccatcagccc cggcaaaggg atgataggaa
     9541 caggattctc tcctgctgct gcctgagccg tggcataggc cctgatctga gggcttgtat
     9601 aaatggcggt cagcttttta tccttcatgg cctttgtgag gcaggcggcc tgggcacgtc
     9661 cggtttcatt taaggggatg tcctgctgtc cctgaatcct gcgctcaata ttccagtctg
     9721 tctgtccatg gcgtattaag tataatttca tgcccttctc ctaactgttt aaatcccagc
     9781 cgtctctcat aggatcatag cccagggatg gacggccgct tcttccatat ccgtctctgc
     9841 ggagagcagg agccggttct gtatcttcct ctgcctcccc gtcagaagcc gccgtacctt
     9901 ccccgtttac cgtcagggcg tccagcgcca gcttcatgat atccttgatc tcatccggcg
     9961 tcatcttcat cttttccgcc agttcctcta cggtagcctc cctgcccagc tcctttgcca
    10021 tgacctggga tacggtctga agcacattca ctctggctgc catcgtttcc tcaatctccg
    10081 cctcgctcct ttgttccagc aaggccgcct caaccgcctg acggatcttc gtttctgcaa
    10141 agtccggcca ctcctggctt ccgtcatact ctgtgacagc catcataaga gccatattgg
    10201 cctcctggac aagatcggaa atgggaagct cttttccctc atattccttt gcaacagcca
    10261 gcgccctagc cagattccct tctaccagac ggtttcctgc ctctttatct gccctggcag
    10321 ctgccgccag gactctcttt tcctcttcct gatccagagg ccggattaac tccatctcct
    10381 ccagatacat ttcataaaaa ttttctgcca tgaatatcta cattcctttt tcattaaaat
    10441 aacatctcta taagaataat ccatccccgc cgccaagtca accactttac aaagcctcac
    10501 aaagctgtta atatagatac agttataagg aggacttaat aatggatata aattatgaat
    10561 tatataaggt gttttaccac gttgccgcca cactcagctt ttcagaggct tccaagcagc
    10621 tctttatctc acagtctgct gtcagccagt ccattaaagt cctggaaaaa aagctgaacc
    10681 agcccctttt catccgaagc accaagcggg tacagctcac ccctgaaggc gaaattcttt
    10741 taaagcatat cgaaccggcc atgaacctga tccgaaaagg ggaaaaccag cttttggagg
    10801 cccataccct gaatggaggc cagctgcgca ttggagccag cgacacgatc tgccgctact
    10861 atctggtaac gtatctgaac cgttttcaca aggagtttcc caatattcac atcaaggtta
    10921 cgaaccacac ttccattgaa tgtgccaagg tgctggaaaa cggacagtgc gattttattg
    10981 tggcaaatta tcccaattct gccctgtcag gcagcctgca cacacgggtg atcaatgaat
    11041 ttcacgatgt attcgtagct aaccgccagg cctttgacct ggagggaaaa tcagtgtccc
    11101 taaaccagct ccttgactac cctattatga tgttagacag aaaaagcaca accagcgaat
    11161 ttctccatca gatctttcag aaaaaccgtc tggatctggt gcctgaaatc gagctttcca
    11221 gcaatgacct tctcattgat ctggctcgga tcggcctggg gatcgccttt gtgccggact
    11281 tctgcatccc ggagcaggat aaggatttat ttatccttaa gctgtccgaa acacttcctg
    11341 ccagacagat ggtagtagcc tacaacgaaa acctgcctgt atcccaggct gccaaacact
    11401 ttatggaaat gctgtagcaa aagaagctta catttcctcc tgccgcggaa atttttccca
    11461 aaattctctc agggcctggg atacatttcc gcggtttact acggtatagg tcccccattc
    11521 cctcggaaaa agggctacat actccgggcg cagaaaacgg tcgtccaaaa gagcgatcac
    11581 tcctttgtca ttcaccgtgc ggatcacccg cccggccgcc tgcatgacct tattcatgcc
    11641 cggatactgg tatgcataat caaacccatt tccctctctc tcgtcaaaat attcttttaa
    11701 aatctcctgc tccgtgttta cctgaggaag tcccgttccc acgataaccg ccccgatcag
    11761 gcgctcctcc tttaaatcaa ttccctcaga aaagatacct cccattacgc agagcgcggc
    11821 aaaagaccgg ctgcgtttcg cttcaaactg ctctaagaac gcctctctct cctcctctgt
    11881 catccggttt gactggcaaa tgcggtcaaa gcccagagag cctgtccttt cccgctccaa
    11941 aaggatagcc tccacctgct cctgaaactg ataggacgga aaaaatacca tataattgcc
    12001 cttccgttcc cggataatga cttccagata gtccgcaatc cgctcgtact ggctccgtcc
    12061 ccgcctgctg taacggctgc tcacatccga cgccgcaagc accagacggt tttcaggtga
    12121 aaacggcgat ttggcataga tcgcatattc ctgcggatca ccgcttaaaa gctccttata
    12181 atattgaacc ggcagcaggg tagccgaaaa aaacaccgta ctttcactct gatccagaca
    12241 ccggcgcaga cattctgacg gatccatgca catgagcttt accataaagc ttccatcctc
    12301 caaaagcctt gcgtagatcc tgtagcgatc atctaaacgg tcatggatat tcaaaaaatc
    12361 acgaacctta aaatagagct ccagcaccag atcccggtcc gtaaattctc cgttttcatt
    12421 cataaaagct tccagctctc caaacatggc catcagctcc agggcaagca gcttcacatc
    12481 acccaaaagc tcataatctg cgcccaaaac tctccggtgg ctgatccctt cttcctcttt
    12541 tcctgtcccg taagctctct tcagctccag catgcgctta ttgcaggcat ccagaagtga
    12601 gactgtcttt gccgctccgg gaagtccctt caggattctt tttgctaaaa gaagatcctc
    12661 cttcacaacg gctgcgctgt acatttccct ggctcttggc accagattgt gggcctcatc
    12721 caccaaaaac aggtaagagc ccgtctgctc cccctcagca aaataacgtt tcagccgcac
    12781 attgggatca aacacataat tgtaatcgca gataatcccg tctgtccagc tgctgatatc
    12841 caggcagaac tcaaagggac atacctgaaa ccgctccgca taccgtaata ccttttctct
    12901 ggtaatcccc tcttcctcat gaatgatctc atagaccgcg tcattgaccc ggtcaaaatg
    12961 gccttttgcc ctgggaca
//
LOCUS       DBMDUH010000068        12891 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_2537_length_12891_cov_5.768022, whole genome shotgun sequence.
ACCESSION   DBMDUH010000068 DBMDUH010000000
VERSION     DBMDUH010000068.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 12891)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12891)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12891
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2537_length_12891_cov_5.768022"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2235)
                     /locus_tag="ACZPCG_07230"
     CDS             complement(<1..2235)
                     /locus_tag="ACZPCG_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HIR1284895.1"
                     /translation="MERYTPQAKEALSLAVGVAESLNHGYVGTEHLLIGLLQEGTGVA
                     AKVLEENGVEEDKVVELVSQLIAPNPTLQTADTAAYTPRARRVIENSYREAVRFKAAQ
                     IGTEHLLIAILREGDCVASRLLNTMGVSVQKIYIDLLAAMGEDAPTVKEEMQSSRAGR
                     RGNATPTLDSYSRNLTKMAEEGKLDPVIGRENEIRRVIQILSRRTKNNPCLIGEPGVG
                     KTAVAEGLAQMIAAGDVPETIADKRVMTLDLSGMVAGSKYRGEFEERIKKVIAEVVDS
                     KDVILFIDEIHTIIGAGGAEGAMDASNILKPSLARGELQLIGATTINEYRKYIEKDSA
                     LERRFQPVTIDEPSEEESVSILKGLRSRYEDHHKVEITDDALEAAVKLSSRYINDRFL
                     PDKAIDLIDEASSRVRLMNYTRPDKVKALEAQIEKLEDEKEAAVREEAYEKAGEIKKK
                     QEKLREKIAALLEKWQKEKETRRLIVSENEIADVVAGWTRIPVKKLAEEESERLKNLE
                     GILHERVVGQEEAVTAVSKAIRRGRVGLKDPKRPIGSFLFLGPTGVGKTELSKALAEA
                     MFGTETALIRVDMSEYMEKHSVSKMIGSPPGYVGYEEGGQLSEKVRRNPYSVILFDEI
                     EKAHPDVFNILLQVLDDGHITDAQGRKIDFKNTIIIMTSNAGAENIISPKRLGFGTAT
                     DAKADYAFMKGRVMDEVKHLFKPEFLNRIDEIIVFHQLNREHMKGIADIMLQGIIKRS
                     REQLG"
     gene            complement(2252..3292)
                     /locus_tag="ACZPCG_07235"
     CDS             complement(2252..3292)
                     /locus_tag="ACZPCG_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP--guanido phosphotransferase"
                     /protein_id="HIR1284896.1"
                     /translation="MSRWYEGIREESSNIIYSRIRLARNWDEYVFPSRLSEQAASQVT
                     EQLREGLKEAETGEHPLLWNDLAAMNGAQKQALRERRILNRTAVEKNGPGGLYLSEDE
                     SCSLLLGADDHIRIQFLAPGLDLEDLWKKADQMDDFINERFSYAFDEKYGYLTTFPTN
                     VGTGLKACVVLHLPMLSQVRKFQSIVGDMGRLGTAIRGLYGEGSENYGNMYELSNQRS
                     LGQSEREIIEQVAKAATQLNNQELRVRNAALNARRLEREDETWKSYGILKYARRITEK
                     DARIFLSQLMAGEEDGVISFEKERSLYSLMIGIKPANLALWASRPLDKEEADCVRAAY
                     IRERLPRMIESA"
     gene            complement(3276..3947)
                     /locus_tag="ACZPCG_07240"
     CDS             complement(3276..3947)
                     /locus_tag="ACZPCG_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003510694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HIR1284897.1"
                     /translation="MLCEKCKIREANIKYTEIINGVKTEHNLCSHCAREMDFGQYGAV
                     LDGEFPLGRLLSGLLGLEEDEEDTDERAAVVCPTCGTSFDDFVENSRFGCPDCYGVFD
                     LFINDKIKQLQGSENHKGKHPKFRSKFEKEHLDALKAPEEPIKGQTTSEDNRIDTDPG
                     KEKTAVSAEESLQVVRLQKALKEAVSKEDYESAARYRDEIRALKAKAAEGRDEKGGRT
                     DEPMV"
     gene            complement(3977..4372)
                     /locus_tag="ACZPCG_07245"
     CDS             complement(3977..4372)
                     /locus_tag="ACZPCG_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007712354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1284898.1"
                     /translation="MVLKIDFNSDEAIYIQLRNQIIMGIATDMIREGDTLPSVRQMAD
                     HIGINMHTVNKAYSVLRQEGFVKLDRRKGAVVCLDIDKLQAIEEMRQNLDVVLACGIC
                     RDISRREVHELVDQIYDRYTRPCADEEDL"
     gene            complement(4581..5606)
                     /locus_tag="ACZPCG_07250"
     CDS             complement(4581..5606)
                     /locus_tag="ACZPCG_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004793 - threonine aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0006567 - L-threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine aldolase family protein"
                     /protein_id="HIR1284899.1"
                     /translation="MLSFESDYTEGAHEAILKRLAETNREQTPGYGADPYCEAAKEKI
                     AGACRCPQADIYFLTGGTQTNQTVIDSLLKNYEGVIAAQTGHVNVHEAGAIEFTGHKV
                     LALPQKNGKLEPETLRAYMEAFYKDPSHDHMVFPGMVYISHPTEFGTLYTKAELTALS
                     EICGEYGLPLFLDGARLGYGLMSLDTDVTLEDIARLCDVFYIGGTKVGALFGEAVVFT
                     KKNAPRQFVTLIKQHGALLAKGRLLGLQFDTLFTDGLYFEISRHAIQMAERLKKGLRE
                     KGCKFLIDSPTNQQFVVLDQDTIRRLEEQVAFSIWEPVDENHMAVRFATSWATTPEMV
                     DALLELL"
     gene            complement(5632..6750)
                     /gene="mobB"
                     /locus_tag="ACZPCG_07255"
     CDS             complement(5632..6750)
                     /gene="mobB"
                     /locus_tag="ACZPCG_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HIR1284900.1"
                     /translation="MKWGCVLLKGGRGSRMGYRDKSELIWRGQTFGFRIRQELEQLGI
                     PCFLSEAEKSSPSEDAGNKMALWNPVLDQIQQAGPMGGIVSCLKACLLKDSSMEGLFF
                     VSCDLPFFRKEMAVRLVAAFKEEDDGVMWRTRDGRLHPVCAFYAKSALPVIESCLNEG
                     EFRMRTLCSRLRMRILDTEKEHIPDTWFMNINRPEAYEALEDRKRPAVLAVSGSKNTG
                     KTTLTEALVRELSKRGVKVAVIKHDGHDFTPDVPGTDSHRMKAAGAFGTVVYSEKRFC
                     VVKEETKQAEDFIACFSEADLILLEGQKHSSYPKLETMRRVISTEPVCRQETVLAYVT
                     DFGGGGIQGKWDETPVYPFQDMDRILELVIKVMDGYKG"
     gene            6939..8789
                     /gene="asnB"
                     /locus_tag="ACZPCG_07260"
     CDS             6939..8789
                     /gene="asnB"
                     /locus_tag="ACZPCG_07260"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015574174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HIR1284901.1"
                     /translation="MYKIAGFFHPEGVYLNNRAYFQERLNAMGDSLKKNGKGQTRLWL
                     SSCICLSETGPSEPETASSLISKAFGSGIVAVAFCGELYNSNELRTELKHLGHSFQSS
                     SDKEILLESYLEWGMDFVKRLNGIFAFAICDTACQTLLLFRDRIGSKPLFYTVLKDGE
                     LAFSSRIRSLLSLPEIKPELDLTGANEIFSLGPARSSGCGIFCGIREVLPGHVLILTP
                     ERKTEKPYWTLESHPHGDSWEKTVEKTSFLVTDAISRQLDSAEPVCTFLSGGLDSSLV
                     SAVCARKCAAVGKTLTTFSFDFTENDACFQSNDFQPSRDRPYAEQMVRCLHTDHHYLE
                     CSSTVLASRLTDSVLAHDLPAMGDIDASLLHFCSMVKNTSSVALTGECADEIFGGYPW
                     FYKEDCLKFHGFPWTMDLSPRKALLKDEIIKLLHMEEYVQSACDFSLSHLPVCKEDTK
                     KEARQREIVWLNLNWFMRTLLERMDRAADFSGLKARVPFADHRIIEYLWNVPWELKAK
                     DHTAKGLLRHVSKGLLPEEVLWRRKSPYPKTYDTHYEALLAEQVREIIHDPSSPVMEF
                     LDREKTERFLQMTSDYGKPWYGQLMAGPQMMAYLIQINFWLSHYRITLKL"
     gene            complement(8797..9999)
                     /locus_tag="ACZPCG_07265"
     CDS             complement(8797..9999)
                     /locus_tag="ACZPCG_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HIR1284902.1"
                     /translation="MYCIKKIKDDLFWVGGTDRRLTLFENAFPIPRGVSYNAYVLKDE
                     KTVLFDTVDNAIGDQLFENLEAVLEGRTLDYVIVNHMEPDHASTLSEVVRRYPEVKIV
                     CNAKTVAIIKQFFDFDIDSRAVIVKENDTFCTGKHTYTFVMAPMVHWPEVMVTYDMTD
                     KILFSADAFGTFGAMNGNIFADEVNFERDWLDDARRYYTNIVGKFGTSVQTLLKKAAG
                     LDIQMICPLHGPVWRENISWYVDKYLTWSSYAPEERAVMIAYGSIYGNTENAANILAC
                     RLADRGVRNIAVYDVSSTHPSVLVSEAFRCSHLVFASVTYNGGIFTNMERVLGDLKAH
                     ALQNRTVALMENGSWGLIAAKQMKEIISTMKNMEILEPSLSIKSSLKKGQEAEIDAIA
                     DAIAASVL"
     gene            complement(10181..11737)
                     /locus_tag="ACZPCG_07270"
     CDS             complement(10181..11737)
                     /locus_tag="ACZPCG_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin-like beta sandwich domain-containing
                     protein"
                     /protein_id="HIR1284903.1"
                     /translation="MIKRIKQGAAALLLMCVMTVLLPWCGITAFAATARIAFSDPSAA
                     AGSEFTVRMKITGSEPLSTADVMLSYDAASLEFIEGTNTDGGNGALRVHGDAGSGGAS
                     TLAYELKFKALTAGTSQITVKEQEVYDADSKLVTVDQQGQSKVTVTAEEGASDDASLK
                     SLKVSPGTLSPEFSADVDTYSVKVGTDVDQLIVSAETADSKAATQVSGAEGLQMGENR
                     VTVKVTAQDGQTSKEYVIVVTKEEGGASADSQTSSANEAFEMHVSRRSFTVMEPDDTV
                     EVPEGLVSTTITIDDHKVTGWVWGDEAEHEYCVIYAMNEDGEKGLYRYDLKSSERTIQ
                     RYFADSSSTAVSAETYELLAGQYDSLRKDYNLFRTLLIAVIVIALVLVVLLIVSLTGK
                     KDRGRRERISERKDETESRPQKEEAPLHSRKEEREKLIPDPFEEEEAEEMEYIVEEDQ
                     GDTSEEEPASKEEDDFFDTIDLSFENEEEEPEHETAEPEPKAPIRQLHPRPSSEEKRE
                     DGDDFEIFDL"
     gene            complement(11803..>12891)
                     /locus_tag="ACZPCG_07275"
     CDS             complement(11803..>12891)
                     /locus_tag="ACZPCG_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin-like beta sandwich domain-containing
                     protein"
                     /protein_id="HIR1284904.1"
                     /translation="GSSQTSGPSGSSSSQNAGSSSGPSSSGSSSGTPSSNTGSGSKLY
                     VDIIMDAAAQSGVSPYVLAAMILQEQGTNGGSPLISGNYSGYPGYYNFFNVEAYQSGS
                     MSAIQTGLRYASQSGSYGRPWNTVEKSIIGGAQNYGDNYVKAGQNTFYLKKFNVQGSN
                     LYKHQYMTNIQGAASEAERLSKAYSSVKDSALEFQIPVYNNMPETACAAPVGDGSPNN
                     KLSSLSAEGYSLTPSFGKDTESYNLIVNTSVSSIQVNAAAADSKASVSGAGSIPLNGS
                     TTDIAITVTAENGSARTYTIHVVKQDGGPVSSGSSGSAGSPSSPGSSEGPGGGNGSSG
                     PGSSGGSGSSGGVNTPGGSNVTIVGVSP"
BASE COUNT         3010 a         3616 c         2729 g         3536 t
ORIGIN      
        1 ccccagctgt tctctgctgc gctttataat accctgcagc ataatatctg caatcccctt
       61 catatgttct ctgtttaact ggtggaacac gatgatctca tcaatgcggt ttaaaaattc
      121 cggcttaaac agatgcttca cttcatccat cacacggcct ttcataaagg catagtcggc
      181 ctttgcatct gtggcagttc caaagcccag acgcttgggg gaaatgatat tttctgcccc
      241 tgcgttggag gtcatgatga taatggtatt tttaaagtcg atcttccgtc cctgggcatc
      301 tgtgatatga ccgtcgtcca gaacctgtaa gagaatatta aatacatccg gatgcgcctt
      361 ttcaatctca tcaaaaagga tcacggaata gggattcctg cgtacctttt cgctgagctg
      421 tccgccctcc tcatatccaa catatcctgg cggagaaccg atcatcttgg aaacactgtg
      481 tttctccata tattcggaca tatctaccct gatcagggct gtttctgtac cgaacatagc
      541 ctctgccaga gccttggaaa gctctgtctt tcccacacct gtcgggccaa ggaataaaaa
      601 ggagccaatg ggacgtttgg gatcttttaa tcccacacgg cctctgcgga tggcctttga
      661 tacggcagtt acggcttcct cctgccccac tacccgctca tggagaatac cttccagatt
      721 tttaaggcgc tccgactctt cctctgccag ctttttcacc ggaatcctgg tccagcctgc
      781 cactacatct gcaatctcat tttcactgac gatcagtctg cgtgtttcct tttccttctg
      841 ccatttctct aaaagggcag cgatcttttc cctcagcttt tcctgctttt tcttgatctc
      901 ccctgccttt tcataagcct cctcccggac agcagcctct ttttcatcct ccagcttttc
      961 aatctgagcc tctaacgctt tgaccttgtc cggacgggta tagttcataa gccgtactct
     1021 ggaagaagcc tcgtcaatca ggtcaatggc cttatccggc aggaagcggt cattgatgta
     1081 ccggctggac agctttaccg cagcctccag ggcatcatct gtgatctcta ccttatgatg
     1141 atcttcataa cggcttctga gcccctttaa gatagaaacg gattcctcct cgctgggttc
     1201 gtcgatggta acgggctgga aacggcgttc cagagcagag tccttttcaa tgtactttcg
     1261 atactcattg atcgtggtgg ctccaatgag ctgaagctcc cctcttgcca gagaaggttt
     1321 taaaatattg gaagcatcca tggctccttc tgctcctccg gcgccaatga tcgtatggat
     1381 ttcatcaata aagaggatca cgtcctttga gtctaccact tcggcaatta cctttttgat
     1441 ccgctcctca aactcacctc tgtatttgga gccagccacc atgccggaca gatccagggt
     1501 catcacccgc ttatccgcaa tggtttccgg cacgtctcct gctgcgatca tctgagccag
     1561 tccttctgct actgccgttt ttcccactcc cggctctccg atgaggcagg gattgttttt
     1621 tgtgcggcgg cttaagatct ggatcacccg gcggatctca ttttcacggc cgatcaccgg
     1681 gtctagcttt ccctcttcag ccatctttgt aagattgcgg ctgtagctgt caagggtagg
     1741 tgtggcattt ccccgccgtc cggctctgga gctctgcatc tcctccttta cagtgggagc
     1801 atcctcgccc atggcggcca gaagatcaat atatatcttc tgcacactga cgcccatggt
     1861 atttaaaagg cggctggcaa cgcagtctcc ctcccgcagg atcgcaatga gcagatgctc
     1921 cgtaccgatc tgggctgcct taaaccgcac agcctcccga tagctgtttt cgatcacgcg
     1981 gcgggctctg ggagtatagg cagctgtatc tgctgtctga agggtaggat tgggagcaat
     2041 gagctggctc accagctcca ctaccttatc ctcttccact ccattttcct ccagtacctt
     2101 ggctgccacg ccggttcctt cctgcaaaag gccaatgaga agatgctccg tacccacata
     2161 gccgtggttt aagctctctg ccacgccaac tgccaggctt aaggcttcct ttgcctgtgg
     2221 tgtatatcgt tccataactt ttatgtcctc cttatgcgct ttcgatcatc ctgggaagcc
     2281 gttccctgat gtatgcggca cgcacacagt cggcctcctc cttatccaga ggccgactgg
     2341 cccacaacgc caaattggcc ggtttgatgc cgatcatcag gctgtataag cttctctcct
     2401 tttcaaagga aataacgccg tcttcctctc ccgccataag ctgggacaaa aagatcctgg
     2461 catccttttc cgtaatcctt ctggcatatt ttaaaatgcc gtaagacttc caggtttcat
     2521 cctcccgctc cagccgtctg gcatttaaag cagcattcct aacccgaagc tcctgattgt
     2581 ttaactgagt ggctgccttt gccacctgct cgatgatttc ccgctccgac tgtcccaggc
     2641 ttctctgatt ggacagctca tacatatttc cgtaattttc gcttccttcc ccgtaaagcc
     2701 cccgtatcgc cgtcccaagc cttcccatgt cgcctacgat gctctgaaat ttgcgtacct
     2761 gagacagcat aggcagatgg agcactacac aggcctttaa gccggttcct acattggtgg
     2821 ggaaagtagt aagataaccg tatttttcat caaatgcata ggaaaaacgc tcattgataa
     2881 aatcatccat ctggtctgct ttcttccaca aatcctccaa atccagccca ggcgcgagaa
     2941 actggatacg gatatgatcg tctgccccta aaagcaggct gcagctctca tcctctgata
     3001 aatataggcc gcctggacca ttcttctcca ctgctgtgcg gttcaaaata cgccgttccc
     3061 ttaaagcctg cttttgcgct ccgttcatag ctgccagatc attccacaaa aggggatgtt
     3121 ctccagtctc cgcctcttta agcccctctc ttaactgctc tgtcacctga gaggccgcct
     3181 gctcacttaa ccgggaggga aacacatatt cgtcccagtt tctggcaaga cggatccggc
     3241 tgtaaatgat attggagctt tcctcacgga ttccttcata ccatcggctc atctgtcctg
     3301 cctcccttct catcccggcc ttcggctgcc tttgctttta aagcacggat ctcatcccta
     3361 taccgggccg cgctctcata atcctcctta gaaacagctt cctttaaggc tttctgcaga
     3421 cgcaccacct gaaggctttc ctctgcagac acagccgttt tttctttccc gggatcagta
     3481 tcgatacggt tatcttccga agtcgtttgc ccttttatgg gttcttccgg cgcttttaac
     3541 gcatccaggt gctctttttc aaatttgctc ctgaattttg gatgctttcc cttatggttt
     3601 tcacttccct gaagctgttt gatcttgtca tttataaaaa ggtcaaacac cccatagcag
     3661 tccggacagc caaaccggct gttttccaca aaatcgtcaa aactggtgcc gcaggtaggg
     3721 cagaccactg ccgccctctc atctgtatcc tcttcatctt cttccagccc taaaaggccg
     3781 gaaagaagcc ttcccagagg aaattctccg tcgagcacag cgccatactg tccaaaatcc
     3841 atttccctgg cacagtgaga acaaagattg tgctctgtct tcactccatt aataatttcc
     3901 gtatatttta tatttgcttc ccgtatctta catttttcac ataacatact gctcctccat
     3961 ccgggctcct gcccatttac aggtcttctt cgtctgcaca aggtcttgta taccggtcat
     4021 aaatctgatc taccagctca tggacttccc gccgggaaat atcccggcag ataccacagg
     4081 ccaaaaccac atccagattc tggcgcatct cctcaatggc ctgaagttta tcaatatcca
     4141 gacagacaac agcgcccttt ctccgatcca gctttacaaa cccctcctgg cgcaaaacgg
     4201 aatatgcctt atttactgta tgcatattga tgccgatatg atcggccatc tgacgcaccg
     4261 agggcagcgt atccccctcc cgtatcatat ctgtagcgat ccccatgatg atctgattgc
     4321 gaagctggat ataaatcgcc tcgtcgctgt taaaatcaat ctttaatacc atacgcacac
     4381 catctttcgt tctatgactc taatcggaac ggacctgccg cagcctgagc atcaacgtta
     4441 tatctgttct acaggcaata taacacctgc cgtctttact ataacaggaa aataaagatc
     4501 cgtcaagaac cactaacttc tggctcctga cggatccctt catttctgca gatattttat
     4561 tcctgccctg ctcctgttct ttaaagcagc tccaaaagcg cgtccaccat ctccggcgtc
     4621 gtagcccagc tggtggcaaa acgcacagcc atatggtttt catctactgg ctcccagatg
     4681 ctgaacgcca cctgttcttc cagccgccgg atcgtatcct gatccagtac cacaaactgc
     4741 tggtttgtag gtgaatcaat aaggaactta cagccctttt cacgaagtcc tttctttaaa
     4801 cgctctgcca tttgaatggc atggcggcta atttcaaagt acagcccatc tgtaaacaga
     4861 gtatcgaact gcagccccag aagacgccct tttgcaagca gcgctccatg ctgctttatt
     4921 aaggtaacaa actgtctggg agcattcttt tttgtaaata ccacagcctc cccaaacagt
     4981 gctcctacct tcgtgcctcc gatataaaac acatcacaga gtctggcgat atcctctaac
     5041 gtcacatccg tatccaggct catcaggcca tatcccaggc gggctccgtc taaaaacaga
     5101 ggaagcccgt actcgccgca gatttcagat agagctgtca gttctgcttt cgtataaaga
     5161 gttccaaact ctgtcggatg ggaaatgtag accatgcccg gaaataccat atggtcatgg
     5221 gatggatcct tataaaacgc ctccatatat gcccgtaacg tctccggctc taactttccg
     5281 tttttctgag gcagagccag aaccttatgg cctgtaaact cgattgcccc tgcctcgtgg
     5341 acattcacat gacccgtctg ggcagcgatc acaccctcat agttctttaa aagagaatcg
     5401 atcaccgtct ggttggtctg agtcccccct gtaagaaaat agatatctgc ctgcgggcag
     5461 cggcaggctc cggcaatctt ttcctttgca gcttcacagt agggatcagc tccatacccc
     5521 ggagtctgct cccggtttgt ctccgccaga cgttttaaaa tggcctcatg ggccccttct
     5581 gtataatcac tttcaaatga cagcatatcg ttctctcctt ttcttatgat tttatccttt
     5641 gtaaccatcc ataaccttta taacaagttc taaaatccga tccatatcct gaaatggata
     5701 caccggcgtc tcatcccatt ttccctgtat ccctccgcct ccaaaatctg ttacataagc
     5761 cagtacggtc tcctgtctgc atacaggctc tgttgaaatg actctgcgca tggtctcaag
     5821 cttgggatag gatgaatgtt tctgcccctc caaaaggatc agatccgcct ccgaaaaaca
     5881 ggcaatgaaa tcttctgcct gctttgtttc ttctttgacc acgcaaaacc tcttttctga
     5941 ataaacaacc gttccaaaag ccccggcagc cttcatgcga tggctgtccg ttccgggaac
     6001 atccggggta aaatcgtgac cgtcatgctt gatcacggcg actttcaccc cacgtttgga
     6061 gagttctctt acaagtgctt ctgtaagcgt cgtttttccc gtatttttac tgccgcttac
     6121 cgccaatacg gcaggccttt ttctatcttc cagcgcttca tacgcctccg gacggtttat
     6181 attcataaac caggtatcgg gaatgtgttc cttttctgta tccagaatac gcatacgcag
     6241 tctgctgcac agcgtcctca tccggaactc cccctcattc aggcaggatt caatcaccgg
     6301 aagggcgctt ttggcgtaaa aagcgcagac gggatggagc cgtccgtccc gtgtgcgcca
     6361 cataaccccg tcatcttcct ctttaaaagc agctaccaga cggacagcca tttcctttct
     6421 gaaaaatggc agatcgcagg acacaaaaaa caggccttcc atggaactgt cctttaaaag
     6481 acaggcctta agacaggaca caatccctcc catgggacct gcctgctgaa tctgatccaa
     6541 aacagggttc cacagagcca ttttgtttcc agcatcttca cttgggctgc ttttttctgc
     6601 ctctgaaaga aaacagggta taccaagctg ctccagctcc tgtctgatcc gaaaaccgaa
     6661 ggtttgtcct ctccatatga gctctgactt atctctgtat cccatacggc ttcctctgcc
     6721 gccttttaaa agaacacagc cccatttcat aatttcgtct cctatccccg gctgccagct
     6781 aaaaagccag cgatcaggca ctatgatgat taagcatatc agtttccggg ttgaaaatca
     6841 agactttcat tctctcgttt ttcccgtttt ctgtcaattt gcagccgtat attgcatatt
     6901 atgattactg aaattatttc ctgcccggag gacagagaat gtacaaaatt gcaggttttt
     6961 tccacccgga aggggtgtac ttaaacaaca gagcttattt tcaggagcgc ctgaacgcta
     7021 tgggagattc cctgaagaaa aacggaaagg gccagacaag gctgtggctc tcttcctgca
     7081 tttgcctttc agaaaccggg ccgtcggagc cagaaaccgc aagcagcctt atctcaaaag
     7141 cctttggttc aggcattgtg gccgtggctt tctgcggaga actttacaat tccaacgagc
     7201 ttcggacaga actgaaacat ctggggcact cctttcaatc ctcctcagat aaagaaatcc
     7261 ttctggaaag ttatctggaa tggggaatgg attttgtaaa acggcttaac ggtatttttg
     7321 cctttgccat ctgtgataca gcctgtcaga cgcttcttct ctttcgagac aggatcggtt
     7381 caaaaccgct gttttacacc gttcttaaag acggtgagct ggccttttcc tcccgtatcc
     7441 gctccctcct ttctctgccc gaaatcaaac cggaactgga tcttacaggc gcaaacgaaa
     7501 ttttctccct tgggcctgcc agatcctcag gctgcggtat tttctgcggc atccgtgagg
     7561 tgcttcccgg ccatgtcctg atccttacgc cggaaagaaa aacggaaaaa ccctactgga
     7621 cgctggaaag ccatccccac ggtgactcct gggaaaagac cgtagaaaaa acctccttct
     7681 tagtcaccga tgctatcagc cgtcagctgg actctgctga accggtatgc acctttttat
     7741 ccggcgggct ggattccagc ctggtatctg ccgtctgcgc caggaaatgt gcagcggtcg
     7801 gaaagaccct taccaccttc tcctttgact ttacggaaaa tgacgcctgc tttcagtcca
     7861 acgatttcca gccctccagg gaccgtcctt atgcagaaca gatggtccgc tgccttcata
     7921 ccgatcatca ttatctggaa tgcagcagta ctgttttagc cagccgcctt acagattccg
     7981 ttttagctca cgatctgccg gccatgggag atatcgacgc ctcccttctg cacttttgct
     8041 ccatggtaaa aaataccagc tccgtggcgc tcacaggtga atgtgccgat gagatcttcg
     8101 gaggttatcc ctggttttat aaagaagact gccttaaatt tcacggcttt ccctggacga
     8161 tggatctgtc gcccagaaag gcgctgctga aggatgaaat aattaaactg ctccatatgg
     8221 aggaatatgt acaaagcgcc tgcgattttt ccctgtccca tctccccgtc tgcaaagagg
     8281 atacaaaaaa agaagccaga caacgggaaa ttgtctggct gaatctgaac tggtttatgc
     8341 gtactctttt agaacgcatg gatcgggccg ctgatttttc cggcttaaaa gccagagttc
     8401 cttttgccga ccacaggatt attgaatatc tctggaatgt gccctgggaa ctgaaggcaa
     8461 aggatcatac ggcaaagggt ctcctgcgcc atgtctccaa gggactgctt cccgaagaag
     8521 tattatggcg cagaaaatcc ccctatccca aaacatatga tacacactat gaagctctgc
     8581 tcgcagaaca ggtgcgtgaa ataatccatg atccctcttc ccctgttatg gaatttttag
     8641 acagagaaaa gacggaacgt ttccttcaga tgacctctga ctatggaaag ccctggtacg
     8701 gccagctcat ggcaggcccg cagatgatgg cttatctgat ccagatcaac ttctggctga
     8761 gtcattaccg cataaccctt aagctatgac gatggattag agaacagaag ctgcaatcgc
     8821 atctgcaatc gcatctattt ctgcctcctg tcccttcttt aagctggatt tgatgcttaa
     8881 ggagggctcc aggatctcca tgtttttcat cgtagaaatg atttccttca tctgctttgc
     8941 agcgatgagg ccccaggaac cattttccat cagggctacg gtgcggttct gaagcgcatg
     9001 tgcttttaag tcgcccagca cccgctccat atttgtgaaa atgccgccgt tataggttac
     9061 ggaagcaaat accagatggc tgcagcggaa ggcttcgctt accagaacgg acggatgagt
     9121 agaggaaacg tcataaaccg caatattgcg gacgcctctg tccgccagtc tgcaggcaag
     9181 aatattggcc gcattctctg tatttccgta aatagatccg taggcgatca taactgccct
     9241 ttcctcaggc gcgtagctac tccaggtaag atatttgtcc acataccagc tgatattctc
     9301 acgccataca gggccgtgga gcgggcagat catctggata tccagtccgg cagccttttt
     9361 taacagcgtc tgaacggaag taccaaactt tcctacgata tttgtataat atcttctggc
     9421 gtcatccagc caatcacgct caaaattcac ttcatccgca aaaatatttc cattcatagc
     9481 gccaaaggta ccaaaggcgt ctgcggaaaa cagtatcttg tctgtcatat cgtaggtaac
     9541 cataacctcc ggccagtgaa ccataggagc cataacaaat gtatacgtgt gctttccggt
     9601 gcagaaggta tcgttttcct ttacgatcac tgcccgggaa tcaatgtcaa aatcaaagaa
     9661 ctgcttgata atagcaactg tcttggcatt gcatacgatc tttacttccg gataacgtct
     9721 tactacctct gacagcgtgg acgcgtgatc cggctccata tgattcacaa ttacatagtc
     9781 cagcgtacgg ccttccagta cagcttccag attttcaaac agctggtcgc cgatggcgtt
     9841 gtccaccgtg tcaaagagaa ccgttttttc atcttttaaa acgtatgcgt tgtaagaaac
     9901 tcctctgggg attggaaatg cattttcaaa caaagtcaga cggcggtctg tcccgccaac
     9961 ccagaataaa tcatctttaa tctttttaat acagtacatc tctaatcttc ctttccaatg
    10021 gacgttaaaa tatacacaaa tttaatccgt aggcagtata gcacaaattg ttttaaatgt
    10081 cattgtatta tttgccgaac agaaggattt ttaaaccggt ttccgataaa aacagcccca
    10141 aaagtaaaga catatcctta catttggggc taaatcctat ttacagatca aaaatttcaa
    10201 aatcatcgcc atcttcccgc ttttcctccg atgacggacg cggatggagc tgacggatgg
    10261 gagccttagg ctcaggctct gccgtttcat gttccggttc ttcttcctca ttctcaaagg
    10321 aaagatctat ggtgtcaaag aagtcgtcct cctccttgga agccggttct tcttccgatg
    10381 tgtcaccctg atcttcctca acgatatact ccatctcttc cgcttcttcc tcttcaaacg
    10441 gatccgggat cagcttctct ctttcttctt tccggctgtg taatggtgcc tcttccttct
    10501 gcggtctgga ctctgtctca tccttccgct cagaaatccg ttctcttctt cctctgtctt
    10561 tctttcctgt cagggacaca ataagaagaa caaccagtac aagagcaatc acgatcactg
    10621 cgatcagcag ggtacggaac agattataat cctttctgag ggaatcatac tgtccggcta
    10681 aaagctcgta tgtctccgca gataccgcgg tagatgagct gtcagcaaaa tatcgctgga
    10741 tggtacgctc agaactcttt aaatcgtatc ggtacagtcc cttttctcca tcctcattca
    10801 tggcataaat tacgcagtat tcatgctctg cctcatcgcc ccatacccat ccggtcacct
    10861 tatgatcgtc aatggtaatg gtggtagaaa ccagtccttc cggcacctct accgtatcat
    10921 ccggctccat cacagtaaag gaacgtctgg aaacatgcat ctcaaaggct tcatttgcag
    10981 aagaggtctg gctgtccgca gatgctccgc cttcttcttt agtaactacg atcacatatt
    11041 cttttgaggt ctgtccgtcc tgtgcagtaa cctttaccgt gacgcggttt tcacccattt
    11101 gaaggccttc tgctcctgaa acctgcgtag ccgccttgct gtctgccgtt tctgcgctga
    11161 cgatcagctg atcaacatcc gtacccactt tcacgctgta agtgtccaca tctgctgaaa
    11221 actccggaga aagggtgccg ggggacacct ttaaggattt caaagaagca tcatcagagg
    11281 ccccctcctc tgcggtcact gtcaccttcg actgtccctg ctgatccact gtaacaagct
    11341 tggaatccgc gtcataaact tcctgctctt taaccgtgat ctgggaggtg cctgcagtaa
    11401 gcgccttaaa tttcagctca taggccagtg tggaggcgcc gcctgatcct gcatctccat
    11461 gaacccggag agctccgttt ccgccgtccg tattggttcc ctcgataaat tccaggctgg
    11521 ctgcatcata ggaaagcatc acatctgccg tagataaggg ttcactgcct gttatcttca
    11581 ttctgacagt aaattcactg cctgcagctg cagacggatc ggaaaatgcg atccgcgccg
    11641 ttgcggcgaa ggctgtgatg ccgcaccatg gcagcagcac cgtcatcaca cacatgagaa
    11701 gcagcgccgc cgctccctgt tttattcgtt ttatcatgat cattcctgct ttctcttatt
    11761 tcatttcttt tccactgcag tttcctgcgc ttatcctgcg gcttaagggc ttacgcccac
    11821 aattgtcaca ttgctgccgc cgggagtgtt tactcctccc gaacttccgg aacctcctga
    11881 tgaaccgggg ccggacgaac cgtttcctcc tcccgggcct tcagaggaac cggggctgga
    11941 aggcgagcct gcacttcctg atgagccaga ggatacaggc cctccgtcct gctttaccac
    12001 atggatcgta taggttcttg cgcttccatt ttcagcagtt accgtaatcg caatatctgt
    12061 ggttgatcca tttaacggaa tacttcctgc tcccgaaaca gacgcttttg aatcagcggc
    12121 agctgcatta acctgaatgc tggaaacaga cgtatttacg atcagattgt aactttctgt
    12181 atcctttcca aaggaaggcg tcagactgta gccctcagcg ctcaggctgg aaagcttatt
    12241 gttcgggctt ccgtcgccta ccggagctgc acaggctgtt tccggcatat tgttgtaaac
    12301 cgggatctga aactcaagag cagaatcctt tacgctgctg tatgccttgg acagccgctc
    12361 cgcctcagaa gctgcaccct ggatatttgt catatactgg tgcttataca gattggaacc
    12421 ttgtacgtta aactttttca gataaaacgt attctgtcct gcttttacat aattatcccc
    12481 gtaattttgg gcgcctccga taattgattt ttccactgtg ttccacgggc gtccgtagga
    12541 accggactga gacgcatagc gaaggcctgt ctgtatggcg gacatggagc cggactgata
    12601 tgcttctaca ttaaagaagt tataatatcc cggatagccg gaataattgc cggaaatcag
    12661 tggggagccg ccgtttgttc cctgctcctg aagaatcatg gctgccagca catagggact
    12721 tactcccgac tgcgctgccg catccataat aatatccaca tacagcttac ttcctgaacc
    12781 cgtattggaa gagggagttc cggatgagct tcctgaggaa gaggggccgc tggaagagcc
    12841 tgcattctga gaggaagaac tgccggatgg accggatgtc tgggaagaac c
//
LOCUS       DBMDUH010000069        12836 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_2554_length_12836_cov_6.751629, whole genome shotgun sequence.
ACCESSION   DBMDUH010000069 DBMDUH010000000
VERSION     DBMDUH010000069.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 12836)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12836)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12836
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2554_length_12836_cov_6.751629"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            128..718
                     /locus_tag="ACZPCG_07280"
     CDS             128..718
                     /locus_tag="ACZPCG_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR1284905.1"
                     /translation="MGTELIDRRIRKTRKQLKDCLISLLKTKRIQDITVRELTEMADL
                     NRGTFYLHYKDVFDLLEQTETELLGKLNSVIQKHRAEELLDRPFNIFHEVYTLVYENA
                     SLVEILLGENGDLNFLNRLKQIMRDKCLHDWMEVVRTESTAVFDAYFAFIVSGCLGLV
                     QHWLKTGLKETPVQMARLTEKIITDQLHVLSQNPLE"
     gene            830..2062
                     /locus_tag="ACZPCG_07285"
     CDS             830..2062
                     /locus_tag="ACZPCG_07285"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HIR1284906.1"
                     /translation="MTKDELYRETACVRIKKGAGRSLKAGGAWIYDNEIDTISGEYKD
                     GDLVTAEDFDGYPLGHGFINTHSKLTVRMLSRKRDAVINDEFIEMRVRNAWEYRKATV
                     DTSSCRVIFGEADFLPGIVIDKFSDVLVVESLALGIDRLKPVILEKLLKILKEEDIHI
                     RGIYERSDAKVRLQEGMDRYKGFIGEAFDTKVEILENGVRYLVDVEDGQKTGFFLDQK
                     YNRLAVQNLCKKTQPERVLDCFTHTGSFALNAGIAGSGHVLGVDASELAVEQARENAR
                     LNGLEDRVHFQCADVFDLLPQLEQRGERFDVVVLDPPAFTKSRSSIKNAVKGYREINI
                     RGMKLVKDGGYLATCSCSHFMDPELFTKTIREAAANVHKRLRQIEYRTQAADHPILWA
                     ADESYYLKFIIFQVCDEK"
     misc_binding    2115..2348
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2388..3710
                     /gene="brnQ"
                     /locus_tag="ACZPCG_07290"
     CDS             2388..3710
                     /gene="brnQ"
                     /locus_tag="ACZPCG_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016439658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HIR1284907.1"
                     /translation="MKLHKKQILLVSFMLFSLFFGAGNLIFPPFLGQNAGSQTPFAIA
                     AFLITAVALPVLGVIVVAKFDGLEKLARRVNPKFAIVFTILIYLSIGPGLGIPRAASL
                     PFEMAVAPYLPEEANLSLFMLGFSLVFFLIAGWLALTPNKLVERIGKFLTPSLLFLLL
                     FVFFNFLLKGNVHVAEPQESYASNSLVKGFLEGYLTMDTIAALNFGLVIATTITGLGI
                     SRKSDVMKYTVMTGIFAGLILAGVYLILAYMGMSSSGVFPIQENGAWTLRCIVHQLFG
                     DTGAVLLAAIFTLACLTTCVGLITSISQYFSTLTSRLSYRQLVLLIAGFSFVVCNQGL
                     NVILSISVPVLDAIYPISIMLIVLGLCDAKLGHIPFIYPCTIGSVGFISVIYAAENIG
                     LDLGVLSPIFHSLPLYSAGLGWTVIAVLVMVVCILTFRFLPARSEIQS"
     gene            3767..4612
                     /gene="pdaA"
                     /locus_tag="ACZPCG_07295"
     CDS             3767..4612
                     /gene="pdaA"
                     /locus_tag="ACZPCG_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004611096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HIR1284908.1"
                     /translation="MKNDGSWDFSFWGIILTGVLFLMAYGAGQWTARQAEAGFFGPSD
                     HLEASADGNWGLGFPEKGKTPVGNASAEYLKQYHAWYAGNPDEKVIYLTFDAGYENGN
                     TEPILDALKKHHAPAAFFLVGNYLETSPELVKRMVSEGHIVGNHTYHHPDMSKISSKE
                     AFQKELSDLETLYEQTTGQKMKRYYRPPQGKYSESNLQMAKDMGYQTFFWSLAYVDWY
                     ENKQPSKEEAFEKLLGRIHPGAVVLLHSTSKTNGQILDELLTKWEEMGYRFESLDHLT
                     AQSAR"
     gene            complement(4597..4956)
                     /locus_tag="ACZPCG_07300"
     CDS             complement(4597..4956)
                     /locus_tag="ACZPCG_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HIR1284909.1"
                     /translation="MKLTILCYDKCSTCKKALNWLDEKGISYEKRPIKEQNPSKEELA
                     VWHKKSGLPLKRFFNTSGNLYKELGLKDKLPQMTEEEQLELLASDGMLVKRPLAVTDQ
                     WAFPGFREKEWEDHLAD"
     gene            complement(4953..5630)
                     /locus_tag="ACZPCG_07305"
     CDS             complement(4953..5630)
                     /locus_tag="ACZPCG_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284910.1"
                     /translation="MKEFTGGIVKTLLFIGLVMFFCPAVVFLGLFAASKLGLTTSRVQ
                     VPLSHVAIDGEEIPIPLQGKVTEGKGWVLRNSKGQYTLTLDGLKAGSLERKTPALEIQ
                     GDLTLVLKKGTESELLSADAALKKGAGILTIRGEGSLRLQGETALEGEWKEETLNQDE
                     PSALRIEGGKLVFTGEETALYEDTVELAGGEIQIQCLNGKGTGIRAHTLDDRHFSGSL
                     EMEESEE"
     gene            complement(5639..6349)
                     /locus_tag="ACZPCG_07310"
     CDS             complement(5639..6349)
                     /locus_tag="ACZPCG_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HIR1284911.1"
                     /translation="MERRIDLAEISDGKLYGAEDMVKADCGDCEGCFACCQGMGDSIL
                     LDPYDICRLSEGLGKSFEELMKEAVGLKMADGLILPYLKMTGEKEACSFLNSEGRCSI
                     HTFRPGFCRLFPLGRLYENGSFRYFLQIHECRKENRTKVRVRKWLDTPDFAAYEQFIS
                     DWHYMTKELGERIRSLGAEGQEKSKKICLYVLNRFYMEPYGETKGFYEEFYRRLEQEK
                     KDLELKNTMYVQERIKQG"
     gene            complement(6382..8592)
                     /locus_tag="ACZPCG_07315"
     CDS             complement(6382..8592)
                     /locus_tag="ACZPCG_07315"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003498632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HIR1284912.1"
                     /translation="MKALVIAEKPSVARDIARVLKCGKNINGAIEGERYVVTWGLGHL
                     VELADPESYDARYKEWRMEDLPMIPDPFKLEVIKQTGKQYQAVKSQLHRKDISQVIIA
                     TDAGREGELVARLILKKAGSRLPIKRLWISSVTDKAIKEGFASLKDGKEYESLRDAAL
                     CRAEADWLVGINSTRALTCKYNAQLSCGRVQTPTLAMIAAREERIRAFVPKPYYGMKA
                     RVQGISFTWQDKAASSSSSFDRARIEGLLSSLKNEPAQAEQIKRTPKKKQPPLLYDLT
                     ELQRDANKQYNYTAKETLNIMQRLYENHKVLTYPRTDSRYLSQDIVPTIKERLKACSV
                     GPYRKIAGTLINRPLAQKMAFVDNSKVSDHHAIIPTEQFVQLDHMTVDERRIYDLVVR
                     RFLAVLYPPCEYEQTEVTVRIGSERFTARANVVTQTGWKTAYEGQSGSWEEDESTEAA
                     ESSADSQKLLSLKEGDVLKGVVLAMTEGKTKPPAPFNEASLLSAMENPAAYMESKDKA
                     MAKTLGETGGLGTVATRADIIEKLFSSFLLEKRGKDIFLTSKAKQLLELVPEDLKKPE
                     LTAQWEMKLSAIAKGDLKRDAFMEEIRDYCKELLGQIKTGEGQFRHDNLTNTKCPVCG
                     KRMLSVKGKNSQMLVCQDRACGHRETVSRTSNARCPVCHKKMELRGKGEGQIFVCSCG
                     HKEKLSAFKDRRKKEGAGVSKKDVARYLNQKKKEETEPLNNAFAAALAGLKVDP"
     gene            complement(8589..9494)
                     /locus_tag="ACZPCG_07320"
     CDS             complement(8589..9494)
                     /locus_tag="ACZPCG_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HIR1284913.1"
                     /translation="MFRKLTAIEPVSLEDWAEEKLHTYAEEVVLYRDIPENDEEIIRR
                     IGDSDAVLVSYTTRIGRNVIEACPQIRYIGMCCSLYSEESANVDIACARERGIRVLGI
                     RDYGDRGVVEFVLSELVRFLHGFDRPMLYDIPTEITGLKAGIIGLGVSGTMIAETLQF
                     MGAEVSYFSRTRKPEKEAAGFAYRPLKELLAESQVVFACLNKNVILLHKEEFDALGKG
                     KLLFNTSIGPAHEVEALAEWLDHEGGYFICDTAGALGDTEGILKDHPNVICAGVSAGR
                     TRQAFGLLSKKVLDNIETCLRGMEE"
     gene            complement(9553..10413)
                     /locus_tag="ACZPCG_07325"
     CDS             complement(9553..10413)
                     /locus_tag="ACZPCG_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033735 - aspartate dehydrogenase
                     [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase domain-containing
                     protein"
                     /protein_id="HIR1284914.1"
                     /translation="MDQTKKKLALLGNGYLSGIVVEAYNMGLLEGYKLTGILGRTKEK
                     TEALAAKAGCRACSTIEELMEDKPDYVAEAASVKSIKDYAKTILCQGASLVVLSIGAF
                     ADQEFYEQAKNCAMEHGTRIYLASGAVGGFDVLRTTSLMALAQKMKAEGLSASEAGSL
                     DGYLTAGISTRKGPASLMGTPVFKDYFMTDPEEAAAFSGSAKEAIGLLPTKVNVAVAS
                     SLATAGPDETTVDITSVPGMAGDDHKITAKIGGPDEVEAVVDIYSRTSAIAGWSTVAV
                     LRNILSPVVF"
     gene            complement(10435..>12836)
                     /locus_tag="ACZPCG_07330"
     CDS             complement(10435..>12836)
                     /locus_tag="ACZPCG_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004075480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HIR1284915.1"
                     /translation="QDQLLGKAEQRRRLVQELKLRETEERELAPVLEKRAVQLKEAEE
                     RTVYCKALDEKIRKGMGQLEQFTALSGLKKELKDKESNLQKIQEEYGLCRKQEELLEE
                     EIQKGKNRQEALAGAEARREQLAYETERTTARKTELVSCGKRYFQLLEAGKQEREAIR
                     LDEEALKEQEAALAKGQEKIDSLMDRDVLLVKKTEEARGLKQLDQVCGRWKEKEETLL
                     KIQEQYRLASVEKSRLRQEYDQFEQLFLDAQAGILADHLKEGERCPVCGSLHHPAPAI
                     RPERVPEKTELEQKKARLSQAEDRVRALCAEAEHGNRECAELGKSIRSGLGTEETDIR
                     PEQAGQILKLRIAALSGQIAQISEEIVLRKKLGRLQEQEQRRWKESQEQYQNRLRRME
                     VIRTQKEQALQDIQTALGREEEEAYILTALKQAVQSLEAELKGLKEKTASNQALVQEF
                     ISLRQILLQKEQALSACRERISRLRAEEASLKAKMDSLREQEKEKNEHLEDWKEETLS
                     VQIESWRREKSGLEKSYEQAAEAFRECNTRFTALQSAVSTLREQNKEAELLSEPEILA
                     CKAVLSEKKEKKAAQRSQLYAEMENNRRIFNAVQGKEAKMAAAEQEYVWMKALADTAG
                     GTLSGKPKIEFETYVQTAFFDRILRRANLRLMTMSSGQYELKRQAAGENKKEKAGLEL
                     NVIDHYNGSERSVKTLSGGETFQASLSLALGLADEIQSNAGGIRLDAMFVDEGFGSLD
                     EEALNQAMKALYSLAEGQRMVGIISHVSELKERISRRITVTKNRKTGGVGSTVQVSGE
                     S"
BASE COUNT         2942 a         3489 c         2762 g         3643 t
ORIGIN      
        1 taatagatta tactaatgaa tgtttaaaat aacaatgatt gatttcacat tcaatgtacg
       61 ttaatcaaca tatttgattt ttatattcat tattatacaa aatcccatac ataaggaggt
      121 tatgaaaatg ggaacagaac tgatcgaccg aagaatccgc aaaacgcgga aacaattaaa
      181 agactgtctt atttctctgt taaaaacaaa gcgtatacag gatatcaccg tccgggagct
      241 gacggaaatg gcagacttaa accgcggaac cttttatctt cattataaag atgtgtttga
      301 tcttctggaa cagacagaaa ctgagctcct tggaaaatta aactctgtga tccaaaagca
      361 ccgggctgaa gagcttttag accgcccctt taatattttc catgaggtgt atacgctggt
      421 atatgaaaat gcgtctctgg tagaaattct cctgggagaa aatggagatt tgaactttct
      481 caaccggctg aaacagatca tgcgtgataa atgtcttcac gactggatgg aagtggttcg
      541 taccgaaagt accgcagtat ttgacgccta cttcgccttc atcgtttccg gctgcctggg
      601 gctggtgcag cactggctga agaccggtct taaggaaacg ccggttcaga tggcgcggct
      661 gaccgaaaaa atcattactg accagcttca tgtgctttcc cagaatcctc tggaataaag
      721 catccccatt aaaatccgca gtttcagtca aaatctctgt agcgcctctg agtaatctgt
      781 ggtataaaag aagatattcc tatggaagaa agctaaagga gagtcctata tgacaaaaga
      841 tgaattatac agagaaaccg cctgcgtacg gatcaaaaag ggagccgggc gttccttaaa
      901 ggctggcgga gcatggatct atgacaatga gatcgacacc atttccggtg aatataagga
      961 cggagatctg gtaacagccg aggactttga cggctatcct ctgggccatg gctttatcaa
     1021 cacacattcc aaactgaccg ttcggatgtt atccagaaaa agggatgctg tcattaatga
     1081 tgaatttatc gaaatgcggg taagaaatgc ctgggaatac aggaaggcca ccgtagatac
     1141 ttccagctgc cgtgtcatct ttggagaagc cgatttcctg ccgggtattg tgatcgataa
     1201 attttccgat gttctggtag tagaatccct agctctggga attgaccgtt taaagccggt
     1261 aatcctggaa aagctgctta agatactcaa agaggaggat atccatattc ggggtatcta
     1321 cgagcgcagc gacgccaagg tacgtctgca ggaggggatg gatcgctata agggctttat
     1381 cggcgaagcc tttgatacca aggtggaaat tctggaaaac ggggtccgct atctggtgga
     1441 tgtagaggat ggacagaaaa ccggcttttt cctggatcag aaatacaacc ggctggctgt
     1501 acagaatctg tgtaaaaaaa cgcagccgga acgggtgctg gactgcttta cccataccgg
     1561 ctcctttgcc ttaaatgcgg gaatcgccgg ttccggccat gttctgggag tagacgcctc
     1621 ggagcttgca gtggaacagg cccgtgagaa tgcccgcctt aacggcttag aggatcgggt
     1681 tcatttccag tgcgccgatg tatttgacct tctcccgcag ttagagcaaa gaggagaacg
     1741 ctttgatgtg gtagttctgg atcctcccgc ctttaccaaa tccagaagtt ccatcaaaaa
     1801 tgcagtaaaa ggctatcgtg aaatcaatat ccgaggcatg aagctggtaa aagacggagg
     1861 ataccttgcc acctgctcct gctcccattt tatggatccg gagcttttca caaaaaccat
     1921 ccgtgaagca gctgccaatg tccacaaacg gctgcgccag atcgaataca gaacccaggc
     1981 cgccgatcat ccgatcctgt gggcggcaga cgaatcctat tatctcaaat tcatcatctt
     2041 ccaggtctgt gatgaaaaat aattttggaa actattgtaa tttctgattt tttattctat
     2101 aatacagcca tagaaaaaat tcatttgata ggaacaagta atcctcagcg gatttttcag
     2161 agagacagcg gctggtgtga gctgtcattg aacctgacgg atgaaatcat cctggagaag
     2221 caaactgaag tttagtagga gcgccggagc agagccacgt tacaggtgat acgcattaga
     2281 ggatgcggag atgagctgga tttcattcag gaattaaggt ggtaccacgg tttttaatcg
     2341 tcctttttat aagggcgatt tttttatttt aaaggaggct ttttattatg aaactacaca
     2401 agaaacaaat cctgctggtg agctttatgc tcttttccct gtttttcggg gcaggcaacc
     2461 tgatattccc gccattttta ggacagaatg caggcagcca gaccccattt gccattgcag
     2521 cctttctgat cacagctgta gcccttcccg ttttgggcgt gatcgttgtg gcgaagtttg
     2581 acggcctgga aaagcttgct agaagggtta atccgaaatt tgccatcgta tttaccattc
     2641 ttatctacct ttccatcggc cccggacttg gtatccccag agcagcctcc cttccctttg
     2701 aaatggcagt ggctccctat cttcctgagg aagcgaattt gtccttattc atgcttggat
     2761 tttcccttgt atttttcctc attgcaggat ggctggctct taccccaaac aagctggtag
     2821 agaggatcgg aaaattttta acaccaagcc tgttattttt actgcttttt gtatttttta
     2881 actttctttt aaagggaaat gtccatgtgg cagagccgca ggaaagctat gcttccaatt
     2941 ctcttgtgaa gggatttctg gaaggctatc tgactatgga cacgattgca gccttaaatt
     3001 tcggccttgt aattgctacc accatcacag gactgggaat cagccgtaaa tcggacgtga
     3061 tgaaatatac ggttatgacc ggtatttttg cggggcttat cctggccggc gtttatctga
     3121 tcctggctta tatgggcatg agttcttccg gtgtattccc gattcaggaa aacggcgcgt
     3181 ggactctgcg ctgcatcgtg catcagctct tcggagatac gggcgccgtc cttctggcag
     3241 ctatctttac gctggcctgc ctgacaacct gcgtgggact gatcacctct atttcccagt
     3301 atttttcaac ccttacctcc cggctgtcct accgccagct ggttcttctg atcgcaggtt
     3361 tttcctttgt ggtctgcaat caggggctca atgtgatctt aagcatttcc gtacctgtac
     3421 tggatgcgat ttatcccatt tccatcatgc tgatcgttct cggactgtgt gatgcaaagc
     3481 tgggacacat tccctttatc tatccctgca ccatcggctc tgtaggattc ataagcgtta
     3541 tctatgcagc tgaaaacatt ggcctggatc ttggagtttt aagccctatc ttccactccc
     3601 ttccgctgta ttccgccggc ctgggatgga ccgtcatcgc tgtccttgtc atggttgtgt
     3661 gtattctcac cttccgcttt ttgccggccc gctctgaaat ccagtcataa taaaacgaag
     3721 ctccctgtat actgaactaa ccgtatacag ggagctgttt gttattatga aaaatgatgg
     3781 atcctgggat ttttcattct ggggaatcat actcacagga gtattgtttt tgatggccta
     3841 cggagccggc cagtggactg ccagacaggc agaagcggga tttttcggcc cttcggatca
     3901 tctggaagcg tctgcagacg gaaactgggg tctgggtttt cctgaaaaag gaaaaacacc
     3961 cgttgggaat gcctctgccg aatatttaaa acagtatcat gcctggtatg ccggaaatcc
     4021 ggatgaaaag gtaatctatc tcacctttga tgcaggctat gaaaacggaa acaccgaacc
     4081 aattctagat gcactgaaaa agcatcatgc acctgcggcc ttctttttag taggaaacta
     4141 tctggaaaca agccccgaac tggtaaaacg gatggtatcg gaaggccaca ttgtagggaa
     4201 tcacacctat caccatcccg atatgtcaaa aatttccagc aaagaagcct tccagaagga
     4261 gctgtccgat ctggaaaccc tctatgaaca gaccactgga cagaaaatga agcgatacta
     4321 ccgcccccct cagggaaaat acagtgaaag caatctgcag atggcaaagg atatgggata
     4381 tcagaccttt ttctggagcc tggcctatgt ggactggtat gaaaacaaac agccctcgaa
     4441 ggaagaggcc tttgaaaagc ttttaggccg catccatccg ggggctgtgg ttctccttca
     4501 cagtacatca aaaaccaatg gacagattct ggatgaactg ctgacaaaat gggaagaaat
     4561 gggatatcgt tttgaatccc tggatcatct tacagctcag tctgccagat gatcctccca
     4621 ctccttttcc cgaaaacccg ggaaggccca ctggtctgta actgccagag ggcgttttac
     4681 aagcatacca tcggatgcaa gaagctccag ctgctcctcc tctgtcatct ggggcagctt
     4741 gtccttaagg cccagctcct tatacagatt cccgctggta ttgaaaaagc gctttaaggg
     4801 aaggccgctt ttcttatgcc agacggccag ttcctccttt gagggattct gttccttaat
     4861 gggtcttttt tcataggaaa tccccttttc atccagccag tttaaggctt ttttacaggt
     4921 actgcatttg tcatagcaca atatcgtcag cttcattctt ctgattcctc catttccaaa
     4981 cttcctgaaa agtgacggtc gtccaatgta tgggccctga tccctgttcc ctttccattc
     5041 agacactgaa tctgtatctc cccgcctgca agctctacgg tatcctcata aagagccgtt
     5101 tcctctccgg taaataccag tttcccccct tcgatccgca gggcagaggg ttcatcctga
     5161 ttaagcgttt cctccttcca ctccccctcc agggccgtct ctccctgaag ccttaagctt
     5221 ccttctccgc gtatggtaag aatacctgcc ccctttttca gggcggcgtc tgcacttaaa
     5281 agttcgctct ctgtcccctt tttcaaaacc agggtaagat ctccctggat ttcaagagcc
     5341 ggagtctttc tttccaggct gcctgccttt agtccgtcca gagtcagtgt gtattggcct
     5401 ttggaatttc tcagcaccca tccttttccc tccgtaacct ttccctgcag gggaatgggt
     5461 atttcttctc cgtcaatcgc cacatggctt aaaggaacct gaaccctgga ggtggtaagc
     5521 cccagctttg acgctgcaaa aagccccaga aatacaacgg ccggacagaa gaacatcaca
     5581 aggccgataa acagaagggt cttcacaatt ccccctgtaa attccttcat attttctctc
     5641 atccctgttt tatcctttcc tgcacgtaca tggtgttttt aagttccaaa tccttttttt
     5701 cctgttctaa ccgcctgtaa aattcctcat aaaacccttt tgtttctcca tacggctcca
     5761 tgtaaaaccg gttcagcaca taaagacaga ttttttttga tttctcctgc ccttctgctc
     5821 ccaaagaacg gatcctttct cccaattcct ttgtcatgta gtgccagtca ctgataaact
     5881 gctcataggc cgcaaaatca ggcgtatcca gccatttgcg tacccgtacc tttgtccgat
     5941 tttccttcct gcactcatgg atctggagaa aataccggaa gcttccgttt tcataaagac
     6001 ggcccagagg aaagagacgg cagaagcctg gccggaatgt atgaatggaa caccggcctt
     6061 ctgagtttaa aaaggaacag gcctctttct cccccgtcat ctttaaatac ggaaggatca
     6121 gtccgtctgc cattttaaga cccacagcct ccttcatcag ctcctcaaag ctttttccaa
     6181 gcccctctga caggcggcag atatcataag gatccagcag aatggaatcc cccatgccct
     6241 gacagcaggc aaagcagccc tcacagtctc cgcagtccgc ctttaccata tcctctgccc
     6301 cgtaaagctt tccatcggaa atttccgcca gatcaatcct tcgttccata ttgttccctc
     6361 tttcttttcc ttccggacat gttacggatc caccttaagc cctgcaagcg ccgcggcaaa
     6421 cgcattgttt aatggttccg tttcttcttt tttcttctgg tttaagtagc gggccacatc
     6481 ctttttggat acgccggctc cttccttctt tctccggtcc ttaaaagcag acagcttctc
     6541 cttgtgaccg cagctgcata cgaaaatctg tccctctccc tttccccgca gctccatttt
     6601 tttatggcat acagggcagc gggcattgga ggttcgggag acagtctctc tgtgaccgca
     6661 ggcccggtcc tggcaaacca gcatctggct gttttttccc tttacagaaa gcatccgttt
     6721 tccgcagacc ggacatttgg tattggtcag gttatcatgg cgaaactgcc cttctcctgt
     6781 tttaatctgc cccagaagtt ccttacagta gtcacggatt tcttccataa atgcatcccg
     6841 ctttaagtct ccctttgcaa tggctgaaag cttcatctcc cactgggcgg taagctccgg
     6901 tttttttaag tcctccggca ccagctctaa gagctgtttt gccttggagg tgaggaaaat
     6961 atcctttccc cgcttttcaa gtaaaaaact ggaaaaaagc ttttcgatga tatctgcacg
     7021 ggtcgctacc gtgccaagtc cccctgtctc tcccagcgtt tttgccatag ccttatcctt
     7081 tgactccata taagcagccg gattttccat ggcagacaga aggctggcct cattaaaggg
     7141 cgcagggggc tttgtcttcc cctccgtcat ggcaagaact actcccttca ggacatctcc
     7201 ttccttcagg ctcaaaagtt tctggctgtc tgcgctgctt tctgctgcct ccgtgctctc
     7261 atcttcctcc catgagccgc tctgtccctc atacgctgtc ttccagcccg tctgggtaac
     7321 cacattggcc ctggctgtaa agcgctcgct gccgatccgt accgttacct ctgtctgctc
     7381 atactcgcag ggcggataca gcaccgccag aaagcggcgg acaaccagat cgtagatccg
     7441 tcgctcatct acggtcatgt gatccagctg gacaaactgt tctgtgggaa tgatggcatg
     7501 gtgatctgaa acctttgagt tatccacaaa agccatcttc tgggcaagag gacggttgat
     7561 cagagtccct gcgatctttc ggtatggccc tacagagcag gcttttaagc gctcctttat
     7621 cgttgggaca atgtcctggc tcaaatatct ggaatccgtt ctggggtacg tcagtacctt
     7681 atgattctca tacagccgct gcatgatgtt taatgtttcc ttggctgtat aattatactg
     7741 tttattggcg tctctctgaa gctctgtcag atcatataaa agcggcggct gcttcttttt
     7801 gggcgttctt ttgatctgct ctgcctgggc aggctcattt ttaagagagg acaaaagtcc
     7861 ctctatccgc gcccggtcaa aggaactgct gctggatgca gccttatcct gccaggtaaa
     7921 ggaaatcccc tgaaccctgg ctttcattcc ataataaggt ttcggcacaa aggctctgat
     7981 ccgctcctct ctggctgcaa tcatggctaa tgtaggcgtc tgcacccgtc cgcaggataa
     8041 ctgagcatta tatttgcagg tcaatgccct ggtgctgttg atcccaacca gccagtctgc
     8101 ctcggccctg cacaatgcgg cgtcccggag ggattcatac tcttttccgt cttttaagga
     8161 ggcaaatccc tctttaatcg ccttatccgt aacggatgag atccaaagcc gtttaatcgg
     8221 cagacggctt cccgcctttt taagaatcag cctcgctacc agctctcctt cccgtcccgc
     8281 atctgtggca atgatcacct gggaaatatc cttccggtga agctgggatt ttactgcctg
     8341 atactgcttt ccggtctgtt tgatcacttc cagcttaaaa ggatcgggaa tcatgggaag
     8401 atcctccatc ctccactcct tatagcgggc gtcgtagctc tccggatccg ccagttccac
     8461 cagatggccc agcccccagg ttactacata gcgttctccc tcaatggctc catttatatt
     8521 ttttccgcat ttcagcacgc gggcaatgtc acgggccaca gaaggctttt cagcaattac
     8581 cagcgctttc attcttccat tcctcttaaa caggtttcaa tattatccag tacctttttg
     8641 cttaaaaggc caaaggcctg tctggttctt ccagcagaaa ctcctgcgca gataacgttt
     8701 ggatgatcct ttaaaatgcc ttctgtatct ccaagggctc ctgcggtgtc gcagataaaa
     8761 taaccgccct catgatccag ccattctgcc agcgcctcca cctcatgagc cggcccaatg
     8821 gatgtgttaa agagaagctt tccctttccc aaagcgtcaa attcctcttt gtgaagcagg
     8881 atcacatttt tattcagaca ggcaaatacc acctggcttt ctgccaaaag ctcctttaac
     8941 ggacggtagg caaagcctgc tgcctccttt tccggcttgc gggtacggct gaaataggaa
     9001 acctccgctc ccataaactg cagcgtctct gcaatcatgg ttccggaaac accaaggccg
     9061 atgataccag cctttaaacc ggtaatctcc gtagggatgt catagagcat ggggcggtca
     9121 aacccgtgaa ggaaccgcac cagctcgctt aaaacaaatt ctaccacgcc ccggtctccg
     9181 taatcccgga tgcccagtac acggattcct ctctctctgg cgcaggcaat atctacattg
     9241 gcgctctcct ctgaataaag gctgcagcac ataccgatat aacggatctg tggacatgcc
     9301 tcaatcacat tccgtccaat ccttgtggta tagcttacta aaaccgcatc tgaatcaccg
     9361 atgcgccgga tgatctcctc atcattttca gggatatctc tgtataatac aacttcttct
     9421 gcataggtat gaagcttttc ctccgcccag tcctccaggc tgaccggttc aatggcagtc
     9481 agtttacgaa acatttctat ctcctttctt ataatccatt ttcggatatt atcattttaa
     9541 atataaagtc ggttaaaata caacaggtga caggatattt ctcagcacgg ctacggtgct
     9601 ccatcctgca atggcgctgg tacgggaata gatatctact actgcctcta cctcatccgg
     9661 accgccgatc tttgccgtaa ttttgtggtc gtctcctgcc ataccgggaa cactggtgat
     9721 atccactgta gtttcatccg ggcctgctgt agcaagagag gaagcgaccg ccacatttac
     9781 cttggtcggc agaagaccga tagcctcctt cgcgctgccg gaaaaagcag ccgcttcttc
     9841 tggatctgtc atgaaataat ctttaaagac cggtgttccc atcagggatg ccggcccttt
     9901 tctggtagaa attccggctg taagataacc gtccagactt ccggcctcag aagcagacag
     9961 cccctctgcc ttcatcttct gtgccagggc catcaaagat gtggtgcgaa gcacatcaaa
    10021 gccgcctacc gctccggatg ccagataaat cctggtgccg tgctccatag cacagttttt
    10081 tgcctgctca taaaattcct ggtctgcaaa tgctccgatg gaaagaacga ccaggctggc
    10141 tccctgacag agaatggttt tggcatagtc tttaatggat tttacagacg cagcttctgc
    10201 cacataatcc ggcttgtcct ccatcagctc ttcaatggtg ctgcaggctc tgcagcctgc
    10261 ctttgcagcc agcgcctctg ttttctcctt tgtccgtccc aggatgccgg taagtttata
    10321 gccctcaagc agtcccatat tatacgcctc gactacaatc ccggacagat atccatttcc
    10381 cagtaacgcc agtttcttct ttgtttgatc catcttaaat tacccctttt tcttttatga
    10441 ttctccgctc acctggactg tgcttcccac accgcctgtt ttgcggtttt tagttacagt
    10501 aatccttcgg ctgatcctct cttttaattc agatacatgg gaaataatcc ccaccatccg
    10561 ctgcccttct gccagactgt agagagcctt catagcctgg tttagagctt cctcatccag
    10621 agagccaaag ccctcatcca cgaacatagc gtccaggcgg attcctccgg catttgactg
    10681 tatctcatct gccagcccca aagccaggga caaagatgcc tggaaagttt ctccgccgga
    10741 gagtgttttc acactgcgtt cacttccatt ataatggtca atgacattta attccagtcc
    10801 tgccttctct tttttatttt ctcctgccgc ctgacgcttt aattcatact gaccgctgct
    10861 catagtcatc aggcggagat tcgcccttcg caggatacgg tcaaaaaacg cggtctggac
    10921 ataggtttca aattcgattt tcggctttcc gcttaatgtt cctcccgctg tatcggccag
    10981 agccttcatc cacacatact cctgttccgc agctgccatc ttcgcttcct tcccctgaac
    11041 tgcattaaaa atacggcggt tattctccat ttccgcatac agctggctcc gctgtgcggc
    11101 ttttttctct tttttttcag aaagtacggc tttacaggcc agaatttccg gctcagaaag
    11161 cagctctgct tccttatttt gctctctcag cgtggaaacg gcagactgta aggctgtaaa
    11221 cctggtatta cattcccgaa atgcttcggc tgcctgctca taggactttt ccagtccgga
    11281 cttttccctg cgccagcttt caatctgtac agagagcgtc tcttctttcc agtcttccag
    11341 atgctcgttc ttctcttttt cctgctccct cagagaatcc attttggctt tcagggaggc
    11401 ttcttccgct ctcagccggc tgatcctttc cctgcaggct gaaagcgcct gttccttctg
    11461 caaaaggatc tgcctgagag aaataaattc ctgaacaagc gcctgattgg aggctgtttt
    11521 ctctttcagt cctttgagtt ctgcttccag gctctggaca gcctgtttca aagccgtaag
    11581 aatataagcc tcctcttctt cccgtcccag agctgtttgg atatcctgta aggcctgttc
    11641 cttctgcgtc cgtattacct ccattcggcg cagacggttc tgatactgtt cctggctttc
    11701 cttccacctt ctttgttcct gttcctgcag cctcccaagt ttttttcgca ggacaatttc
    11761 ctctgaaatc tgagcgatct gtccggaaag cgctgcgata cgaagtttca ggatctgccc
    11821 cgcctgctcc ggtcttatat ccgtctcctc cgttcccaga ccagaccgga tgctcttgcc
    11881 aagctctgca cattcccggt tcccatgctc tgcctctgcg caaagcgccc gaacccgatc
    11941 ctcagcctgg ctcagcctgg cttttttctg ctccaattct gtcttttccg gaacccgttc
    12001 aggccttatg gcaggcgccg gatgatgaag agagccgcag accggacatc tttccccttc
    12061 ttttaaatga tctgccaaaa tccccgcctg tgcatccaaa aagagctgct caaactggtc
    12121 atactcctgt ctgagacggg atttttccac acttgccaga cggtactgct cctgaatctt
    12181 taaaagcgtt tcctcttttt ccttccagcg gccacagacc tgatctaact gctttaatcc
    12241 tcttgcttcc tctgtttttt tcactaaaag cacatcccgg tccatcaggc tgtcgatttt
    12301 ttcctgtccc tttgcaagag ctgcttcctg ttcctttaat gcctcctcat ccagacggat
    12361 tgcctcccgc tcctgcttcc cggcctccaa cagctggaaa taccgcttcc cgcagcttac
    12421 cagttctgtt tttctggccg tcgttcgctc tgtttcgtag gctaactgct cccgccttgc
    12481 ttctgctccc gcaagcgctt cctgcctgtt tttccctttt tggatttctt cttccaaaag
    12541 ctcttcctgc ttcctgcaaa gcccatactc ttcctggatc ttttgcagat tgctttcctt
    12601 atccttaagt tccttcttca gaccagaaag cgccgtaaac tgctctaact gccccattcc
    12661 cttccgtatc ttttcatcaa gcgctttgca gtaaactgtc ctttcttctg cttccttaag
    12721 ctgcacggca cgtttctcca gaaccggagc cagttccctt tcttctgttt ccctaagctt
    12781 taactcctgt accagcctgc gcctctgttc tgcttttccc agaagctgat cctgtt
//
LOCUS       DBMDUH010000070        12697 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_2576_length_12697_cov_6.894411, whole genome shotgun sequence.
ACCESSION   DBMDUH010000070 DBMDUH010000000
VERSION     DBMDUH010000070.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 12697)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12697)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12697
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2576_length_12697_cov_6.894411"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="ACZPCG_07335"
     CDS             <1..645
                     /locus_tag="ACZPCG_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284916.1"
                     /translation="AVLGISAVRMTGTWIKARKEAGNRWAHKSLCFLAVPFFLFNTLV
                     SCTTSWAGTPGFTPVKWTGPGCYNHRTEQNERKIRRGYEALAASFSPRDRVLAFGYHP
                     EVLDLNCCVQSYYDVTGSGGDVYLVKKLAYFEEFLEYAGVDYFFVQAGYLADRARAFE
                     IVEDMIEEGTLRDLTFEWGNMKARVALDEKTEELQTEEAERFRQNYCMRENGFR"
     gene            685..2547
                     /locus_tag="ACZPCG_07340"
     CDS             685..2547
                     /locus_tag="ACZPCG_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284917.1"
                     /translation="MEAKDRIKFRIGFSRMEKKYEWKDHLIFMGLLFGLAVWVNRGIE
                     IKGLYMDDLYFWSCYGEQSFLQYVFPMGSTRFRFLYYLAAWLEMAVVGNHVSWFVPIN
                     ILLNGALACYLYGIACRLSGAKAIGFFTGVMFLSSRMAYYQIGQVLGLMETMALWMAA
                     AILWNLYRYINEDHRDSLFLKACLLYFGVCFVHERYMALFPLLILALIAVRCRRPVCI
                     VGAVGSFGLVQLIRAFTIGTVLPAGTGGTQVAETLNIPQAIGFAFSQAAYIFGINAGP
                     EHLNGCPWGQSPFWVKLLVILADLAVLAIVLGFLVMLFGRKRKEKRAALWVNVLLFTG
                     FIALNVASSSVTIRVELRWIYVSLAGALLFLAWMYGELTNGVKKELYLKRIWPWGVLF
                     GAYVLFMFPVELFYRGCYPKLYLWPDQLRYNSLAEETYEKYGDEIFGKTIYIMEDTYE
                     MSDFTARTFFKVFDKKRLAEGTQVKKIESIRDIGLVDDSMLVLREDPEHNGFQDITDF
                     VRDLKLKVDYGYYEDGWMDEHASLTVMAGEEGIIDLEIMYPGIMSGGEMITITKDQEE
                     PLKLPLHFSVVDARLTASPWQLVHLTFDYNFYMQNAMEQRGEDRLAAIVHISAE"
     gene            2585..4057
                     /locus_tag="ACZPCG_07345"
     CDS             2585..4057
                     /locus_tag="ACZPCG_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284918.1"
                     /translation="MKAETNRKDVYRKALCLLIPAAGLAFLLWYIKNATCDVVYSDYI
                     RLVNSYLPDVYDPDRFFVPDVLTRIPINYLGRIINVELFGFSITFDRVLGAVSTALAA
                     WVFGLYFIRHKLGSIWFFLIMAVTFSLNQWEMLTNGSGWSHFFAFACFYYHELVFDRV
                     WTGQEKKGDTLRMILLPVLIILGTAGPYCAIYAVVMILASTAGIVRDLWRKKQKQSEC
                     SALEGEKGLGSKGDFRRYLAFLFAVLIPLLLYILSNSFAVEEHAGATGRSLGELLMDA
                     PSFPVRFLLKSLAGMLIGGEELIQWISDGKITDGFCYLLGLFVAAGYLWALWMNLRHK
                     IYERSLFPMMLLTGGALNHVLIFLSRYIFEKENYALSSRYALQFQVGILGIILTFALA
                     VQMKSRLSAAGRIFGAVFCIAILLGNGYTTYREIEKAPYREERFEMMRELAPLMPHLT
                     DEEMMEMNPAAPDLYEYRKGTDKLRKAFEILEENQLNVFR"
     gene            4071..4868
                     /gene="rfbF"
                     /locus_tag="ACZPCG_07350"
     CDS             4071..4868
                     /gene="rfbF"
                     /locus_tag="ACZPCG_07350"
                     /EC_number="2.7.7.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007717315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047343 - glucose-1-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HIR1284919.1"
                     /translation="MKVVILAGGFGTRISEESHLKPKPMIEIGEKPILWHIMKYYSEF
                     GYHDFVICLGYKQYVVKEFFADYFLHTSDVTFDLANNRMEVHNNYSEPWKVTLVDTGL
                     NTMTGGRVKRIQPYIGNEPFMLTYGDGVSNVDLNALADFHKSHGKIATITTVNLGQSK
                     GVLNIDENDSVLSFREKDDNDGALINGGFMVLNPEVFDYLKDDTTVFEREPMERLAAE
                     GQMKSFYHSGFWQCMDTQREMKKLEALWQSGNAPWKIWKDDRKDLKP"
     gene            4865..5926
                     /gene="rfbG"
                     /locus_tag="ACZPCG_07355"
     CDS             4865..5926
                     /gene="rfbG"
                     /locus_tag="ACZPCG_07355"
                     /EC_number="4.2.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047733 - CDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glucose 4,6-dehydratase"
                     /protein_id="HIR1284920.1"
                     /translation="MTIEELKEFYGNKKVLITGHTGFKGSWLTRILTLSGAEVTGYSL
                     TPPTDPSLFEIAGLEDTIKSVVGDIRDLEHLSRVFEETRPEIVFHLAAQPIVRESYQE
                     PVYTYETNVMGTVNVLECVRRTESVRSFLNVTTDKVYENREWEYGYRECDNLDGYDPY
                     SNSKSCSELVTHSYKKSFFEDGRCAISTARAGNVIGGGDFAADRIIPDCVRAAAAGKT
                     IIVRNPYSTRPYQHVMEPLAVYLTIAMKQYEDRTFEGYYNVGPDDKDCVTTGTLADLF
                     CAAWGQGQTWENRWMGGPHEANFLKLDCSKIKHTFGWTPRYGVKEAVEKTVEWSKEYL
                     EGADMTEVMDRQIREFFIS"
     gene            6010..7359
                     /gene="rfbH"
                     /locus_tag="ACZPCG_07360"
     CDS             6010..7359
                     /gene="rfbH"
                     /locus_tag="ACZPCG_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein RfbH"
                     /protein_id="HIR1284921.1"
                     /translation="MMFEHMTEEQARAQILEQVAGYCDTFHKKAPYREGDRIPYASRV
                     YDHDEMVNLVDSALEFWLTSGRYTDEFEKKLAGYLGVKYCSLVNSGSSANLLAFMTLT
                     SPLLGERRIKRGDEIITVACGFPTTVAPAIQYGAVPVFVDVTIPQYNIDVTMLEAARS
                     EKTKAVMIAHTLGNPFNLRAVKAFCDKYDLWLVEDNCDALGTQYTMEEDGSEVTRFTG
                     TIGDIGTSSFYPPHHMTMGEGGAVYTDNGLLNRIMRSFRDWGRDCVCPSGRDNMCGHR
                     FDRQYGELPLGYDHKYVYSHLGYNLKATDMQAAIGCAQLDKFPSFVERRRHNFDRLKA
                     ALAGTEDKLILPEACEHARPSWFGFLITCKEGTDRNQVVQHIEKMGVQTRMLFAGNLV
                     KHPCFDEMRKNGEGFRVVGSLEHTDRILKDTFWVGVYPGMTDEMIDYMAKTIREAIA"
     gene            7383..8225
                     /locus_tag="ACZPCG_07365"
     CDS             7383..8225
                     /locus_tag="ACZPCG_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HIR1284922.1"
                     /translation="MAQTYDMTKVHEANLKILKELDRICRKYKIRYALDAGTLIGAVR
                     HKGFIPWDDDADVVLIRSQYEALKKVIKRELPDTMELLEPDSYRGGRAFYDFTPRIIY
                     KNSQVHPDSDRMDYYEGKLNHLWVDLFILDKLPEGKLASEMTRFIHKAIYGLAMGHRH
                     DLDYSRYSLLHKIFVGGLATAGKLIPLKWIISMQKWAALKDRKSKGSRWYYSNYQPDY
                     LYVTLEDDWCTKTEDAPFEDTQLMIPKGYHQVLTEVYGDYMQLPPEDKRVPTHSSQQI
                     RVFD"
     gene            8249..9205
                     /locus_tag="ACZPCG_07370"
     CDS             8249..9205
                     /locus_tag="ACZPCG_07370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006778536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1284923.1"
                     /translation="MSEDRSNLKKKILSVVVSVYNEEQALKECYTEMKRVLDTLSWDH
                     ELIFVNDGSSDGSLEVLKALASEDQKVKLISFSRNFGHEAAMIAGLDYSTGDGVVCMD
                     ADLQHPPECLPRIIRAFEEGNEVISMVRTRNKSAGLIKNITSSGFYWLINCISDVHFE
                     PNASDFFAVSRSVAEVLKGNYREKVRFLRGYVQNVGFQKTTLEYEAGVRVAGESKYSL
                     KKLFIFSVNTILCFSNMPLKLGIYAGILSGAAGMAVLVYTLFTRQGAPSGYATIVVLI
                     CFMFAMMFLLIGIIGEYIAILFTELKDRPVYIVRETANIEHN"
     gene            complement(9292..9780)
                     /locus_tag="ACZPCG_07375"
     CDS             complement(9292..9780)
                     /locus_tag="ACZPCG_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284924.1"
                     /translation="MRESMKNNGQTRLVRRCRLRIFLGAAALILGAAALTAALASSAG
                     ASPILYLEPESRSFMTSFYYGTGLGLIIAGVLLILRNRSYLKDPERRRQQEIREFDER
                     SQAIGTKSWAYAGYSLFILLYVMILLSGFVSVLMVKLLLCIMAAFAGLLIIWRMILSR
                     RM"
     gene            complement(9777..9983)
                     /locus_tag="ACZPCG_07380"
     CDS             complement(9777..9983)
                     /locus_tag="ACZPCG_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006780763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR1284925.1"
                     /translation="MKNRIQELRRTGRIRQEDLAEAVGVTRQTIISLENGRYNASLGL
                     AHKIARYFGRTIEEIFIFEEEDDI"
     gene            10268..10900
                     /locus_tag="ACZPCG_07385"
     CDS             10268..10900
                     /locus_tag="ACZPCG_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR1284926.1"
                     /translation="MPTQRFFKLKEEKRRLILEAAAHEFSRVPYSAASINQIIKEADI
                     SRGSFYTYFEDKDDLMRYMLRGMREHFRERVMLLLKEMGSPFAVCLQMVREWMDKGND
                     HIFYKIHKNLLSDLSVSNQNQIFGFRGFTLKDDPYEGFIKDIYEHMDREHYPMSLEAL
                     IYGADVAAMVCIRAMSLYCQKPQEKESILQATETEMRILEKGVCSLQAAK"
     gene            10941..12161
                     /locus_tag="ACZPCG_07390"
     CDS             10941..12161
                     /locus_tag="ACZPCG_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR1284927.1"
                     /translation="MKNQKRKTAGRHMAAITAAILTMSFPMNILAASNRPDDNTYQAP
                     EAPAPNYNKDMSPEFAYTEEKWASLRDNVMEYGELKDLIHEYNPTVRSNRSAYKDQKG
                     KDLNDVYEQYMNDIDDIWDQADAAEDDVTWASMRFSAGLLTKQADNNYQDADMEKIQY
                     DQQEAALVYQAQQMMVSLQQAEDNIKNLENTKALLQSQYEFTRAQAAAGMATQIDVLT
                     AQKSVQDQDTAILAAQKNKENVHRNLCLMLGWDADAQPEILAVPEPDMSRIDILNPDG
                     DAEAALANDYDVRYYEKKAGNLSSQDLIESNQASIQNAKDTAVRSLKTQYNTVLTARD
                     ALNAAQSQLAAEQSKLNKASASLAAGQITNLEYQTTLNAVMTAESDVNTKKLQLLVAL
                     EGYDWNVKGLTTSN"
     gene            12186..>12697
                     /locus_tag="ACZPCG_07395"
     CDS             12186..>12697
                     /locus_tag="ACZPCG_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284928.1"
                     /translation="MKTTFLKRTAAASLSVVLAASVPFTVLADQTDPNVAYEQEQQLL
                     SDGHLDYGEIEERVKNYYGPMKSAYDMAKGMVEDQADIAVNERIMASDLLSQADVAED
                     MAEEQTGMDQAISAATAKALRQSARQMRNAASMMGQSLKKTSSTERQVDRQANSLIMN
                     VQSMMNQYEQL"
BASE COUNT         3353 a         2744 c         3438 g         3162 t
ORIGIN      
        1 gcggttcttg gaatcagcgc agtgcgcatg acaggaacgt ggataaaagc ccggaaagag
       61 gcagggaatc gatgggctca caaaagcctc tgttttctgg cagttccatt ttttctattc
      121 aatacgctgg tatcctgcac caccagctgg gccggcacac cgggctttac ccctgtgaaa
      181 tggaccgggc caggctgcta taaccacagg acagaacaga acgagcggaa aatacgccgg
      241 ggctatgaag cgctggcagc cagcttttca cccagagacc gggtactggc ttttggatac
      301 caccctgagg tgctggattt aaactgctgt gttcagagct attatgatgt gaccggcagc
      361 gggggagatg tttatctggt aaagaagctt gcgtattttg aagaatttct ggaatatgca
      421 ggggttgact atttctttgt ccaagctgga tatctggccg atcgggcaag ggcctttgaa
      481 atcgttgaag acatgatcga agagggaacc ctgcgggatc ttacctttga gtggggaaat
      541 atgaaagccc gtgtggcttt agatgaaaaa acagaggagc ttcagacaga ggaggcggag
      601 cgcttccgcc aaaactactg tatgagagag aacggattcc ggtaggcgtc cggcaaacac
      661 acctgaataa aggaggcaga agcattggaa gcaaaggaca gaataaaatt caggatcgga
      721 ttttccagaa tggagaaaaa atatgagtgg aaggatcatc tgatctttat gggactgctt
      781 ttcgggctgg ctgtgtgggt caaccgggga atcgagatca agggccttta tatggatgac
      841 ctgtatttct ggtcctgcta cggagagcag agctttctgc agtatgtatt tcccatggga
      901 agtacccgtt tccgcttcct ttattatctg gcagcctggc tggaaatggc tgtggtggga
      961 aatcatgtgt catggttcgt acccattaac atccttttaa acggagctct ggcctgctat
     1021 ctgtatggaa ttgcctgcag gctttccgga gcaaaggcca ttggattttt tacaggcgtc
     1081 atgtttttat ccagccgtat ggcctattat cagatcggac aggtgctggg gctgatggaa
     1141 accatggcgc tgtggatggc agcggcaatt ttgtggaatc tgtaccggta tattaatgaa
     1201 gatcacagag acagcctgtt tttaaaggcc tgtctgctgt attttggtgt ctgctttgtc
     1261 catgagcgtt atatggctct ctttcctctt ctcattctgg ccttgatcgc ggtccggtgc
     1321 agacggcctg tctgcattgt gggagcggtg ggaagcttcg ggctggttca gctcatccgg
     1381 gcctttacca tcggtacggt gcttccggca ggaacgggag gcactcaggt ggcagaaaca
     1441 ttaaatatcc ctcaggccat tggctttgcc ttcagtcagg cagcttacat tttcgggatc
     1501 aatgcgggac cggagcactt aaacggctgc ccctggggcc agagcccttt ttgggttaag
     1561 cttttggtga tcctggcaga tctggcggtt cttgccattg tgcttggttt ccttgtaatg
     1621 ttgtttggaa gaaaaaggaa agaaaaacgg gcggctcttt gggtaaatgt ccttttattt
     1681 acgggcttta ttgcgcttaa tgtggcatcc tccagcgtta cgatccgtgt ggaactgcgc
     1741 tggatctatg tatccttagc gggtgcattg ctgtttctgg cctggatgta cggcgagttg
     1801 acaaatggag tgaaaaaaga gctttatctg aagcgcatct ggccctgggg cgtgctcttt
     1861 ggagcctatg tgcttttcat gttccctgtg gagctttttt acagaggctg ctatccaaag
     1921 ctttatctct ggccagatca gctgcgctat aattctctgg cagaagaaac ttatgaaaaa
     1981 tatggggatg agattttcgg aaaaaccatc tatatcatgg aagacaccta tgagatgagt
     2041 gattttacag ccagaacctt ctttaaggtc tttgacaaaa agagactggc cgagggaacg
     2101 caggtaaaga agattgaaag catccgcgac atcggactgg tggatgacag tatgctggtg
     2161 ctgcgggagg atccggagca caatggtttt caggatatta cagattttgt aagggattta
     2221 aagctcaagg tggattacgg ctactatgag gacggctgga tggatgaaca cgcctctctg
     2281 accgtgatgg cgggagaaga agggatcatt gatctggaaa tcatgtatcc cggcattatg
     2341 agcggcggag aaatgattac cattacaaag gatcaggagg agcccttaaa gcttcccctt
     2401 cacttcagtg tggtggatgc gcgccttacg gccagcccct ggcagctggt gcacctgacc
     2461 tttgactata atttttatat gcagaatgcg atggagcaga ggggcgaaga ccggctggca
     2521 gctattgttc acatttcggc agaataaagc cggaaatagc cgggaatttt ggaggtaacg
     2581 aatcatgaag gcagaaacaa acagaaaaga cgtatacaga aaggcgctct gccttctgat
     2641 tcccgcagcg gggctggcgt ttcttttatg gtacatcaaa aatgccacct gcgatgtggt
     2701 ttattccgat tatatccgcc tggtaaacag ctacctgccg gatgtctatg atccggatcg
     2761 cttttttgtg cccgatgtcc tgacaagaat tcctattaac tatctgggac ggatcatcaa
     2821 tgtggagctg tttggctttt ccattacttt tgaccgggtt ctgggggctg taagcacagc
     2881 gcttgcggcc tgggtattcg gcctgtattt tatccgtcac aagctgggga gcatatggtt
     2941 ttttcttata atggccgtga cattcagcct gaatcaatgg gagatgctta caaatggaag
     3001 cggctggtcc cacttttttg cctttgcctg tttttattac catgaattgg tttttgaccg
     3061 ggtgtggacg ggacaggaga agaaaggaga tacccttcgc atgatcctgc tgccagtact
     3121 tattatcctt ggaactgcag ggccgtactg cgcaatctat gcggtggtaa tgatcctggc
     3181 ttcaacagcg ggaattgtaa gagacctgtg gagaaaaaaa caaaagcagt cagaatgttc
     3241 cgctttggaa ggggaaaaag ggctgggatc taagggggat tttcgcaggt atctggcatt
     3301 tctttttgca gttttgatcc cccttctgct ttatatcctc agcaattcct ttgcggtgga
     3361 ggagcacgcc ggagcgacgg gccgttccct gggagagctt ttaatggatg cgccctcctt
     3421 tccggtgcgt tttcttttaa agtctctggc gggaatgctg atcggaggag aggagctgat
     3481 ccagtggatt tcagacggga aaatcacaga tgggttctgt tatcttttgg ggctttttgt
     3541 ggcggcaggc tatctgtggg ctttatggat gaatctgcgg cacaaaattt atgagagatc
     3601 ccttttccct atgatgctcc ttacgggagg cgccttaaat catgtgctga tctttttatc
     3661 cagatacatt tttgaaaagg aaaactatgc gttaagctcc cggtatgccc tgcagtttca
     3721 ggtggggatt ctgggaatta tcctgacctt tgccctggca gtacagatga aaagcaggct
     3781 ctctgcagcc ggccgcatct ttggggctgt attctgtatt gcaatccttc tgggaaatgg
     3841 ttatactaca tatcgtgaga tagaaaaagc gccttaccgg gaagaacggt ttgagatgat
     3901 gagggagctt gcccccctga tgccgcacct gaccgatgag gagatgatgg agatgaatcc
     3961 cgcagccccg gatctgtatg aataccgcaa gggaacggac aagctgcgaa aggcatttga
     4021 gatattggaa gagaatcagt tgaatgtatt tcgttaatgg aggagattcg atgaaagttg
     4081 taatattagc agggggcttc ggcacccgga tcagtgagga aagccactta aaaccaaagc
     4141 ccatgatcga gattggtgaa aagcccatcc tgtggcacat tatgaagtat tattccgaat
     4201 tcggctacca tgattttgtg atctgtctgg gctataagca gtatgtggta aaggaatttt
     4261 ttgcagacta tttccttcac acctcagatg tgacctttga cctggcaaat aaccggatgg
     4321 aggttcacaa taattattcc gagccgtgga aggtaactct ggtggatacg ggacttaaca
     4381 ccatgacagg aggccgtgta aagcggattc agccctatat cggaaacgag ccctttatgc
     4441 ttacatacgg ggacggggtt tctaatgtgg acttaaatgc cctggcagac tttcacaaat
     4501 cccatggaaa gattgccacc attaccactg taaacctggg ccagtcaaag ggcgttttaa
     4561 atattgatga aaatgacagc gtactttcct tcagggaaaa ggatgacaat gacggagcgc
     4621 tgatcaacgg cggctttatg gtgctgaatc cggaagtatt tgactattta aaggatgata
     4681 ctacagtttt tgagagagaa cccatggagc ggctggcggc agagggacag atgaaatcct
     4741 tctatcacag cggattctgg cagtgtatgg atacacagag agagatgaaa aagctggagg
     4801 ccttatggca gtcgggaaat gcgccctgga aaatatggaa ggatgacaga aaggatctta
     4861 agccatgaca attgaggaat taaaggaatt ttatggaaat aaaaaagttc tgatcaccgg
     4921 acacacgggc tttaaaggct cctggcttac ccgcatcctg actctttccg gggcagaggt
     4981 tacgggctat tccctgactc cgcctacgga tcccagcctt tttgaaattg ccggtctgga
     5041 ggacaccata aagagtgtgg ttggagatat ccgcgacctg gagcatttaa gccgcgtatt
     5101 tgaggaaacc cggccggaaa tcgtatttca tctggcagcc cagcccattg tgcgggaatc
     5161 ctatcaggag ccggtttaca cctatgaaac aaatgtaatg ggtacggtaa atgtattaga
     5221 gtgcgtccgc cggacggaga gcgtgcgttc ctttttaaat gtgactacgg ataaggttta
     5281 tgagaaccgg gagtgggagt atggctaccg ggagtgtgac aatctggatg gctatgatcc
     5341 ctattccaac agcaaatcct gctccgagct tgtgacccac agctataaaa aatccttttt
     5401 tgaggacgga cgctgcgcca tttctaccgc acgggcggga aatgtgatcg gcggaggcga
     5461 ttttgccgca gacaggatca ttccggactg tgtgagggca gctgcggcag gaaagaccat
     5521 tattgtccgc aatccctatt ccacccggcc atatcagcat gtgatggagc ctctggctgt
     5581 ttatctgacc attgccatga agcagtatga ggacagaacc tttgaggggt attataatgt
     5641 aggcccggac gacaaggact gtgtgacaac gggaacgctg gcagatctgt tctgcgcagc
     5701 ctggggacag ggacagacct gggagaaccg ctggatgggc ggcccccatg aggcgaattt
     5761 cctgaagctg gactgctcaa agatcaagca tacctttggc tggacgccca gatatggcgt
     5821 aaaggaagca gtggagaaaa ctgtggaatg gtcaaaagag tatctggagg gagcggatat
     5881 gacagaggtg atggatcgcc agatccgcga atttttcatt tcataggaaa gggcgtccta
     5941 tggaataaaa atgctccgcg gggagtgccc tgcggcgttc ctatgaaata gaatcaagga
     6001 ggaaaacata tcatgtttga acatatgacc gaagaacagg caagagcgca gattctggag
     6061 caggtagccg gatactgcga tacctttcat aaaaaggcgc catacagaga aggcgacagg
     6121 attccctatg cgtcccgcgt ttatgaccat gatgagatgg taaatctggt ggacagcgct
     6181 ctggaattct ggctgacttc cggacgttat acggatgaat ttgagaaaaa gctggcagga
     6241 tatctgggag taaaatactg ctcgctggta aattccggct catctgccaa tcttcttgcc
     6301 tttatgaccc tgacctctcc gctgcttgga gagcgccgca tcaaacgtgg ggatgagatc
     6361 atcaccgtag cctgcggctt tcctaccacc gtggctccgg ctattcagta cggcgccgtt
     6421 cctgtattcg tggatgtaac cattcctcag tataatattg atgtaaccat gctggaagcg
     6481 gcccggtctg aaaagacaaa ggcagtgatg atcgcccaca ctctgggaaa tccatttaac
     6541 ctgagggcgg taaaggcctt ctgcgacaaa tatgatctgt ggcttgtaga ggataactgt
     6601 gatgctctgg gaacccagta taccatggag gaggatggaa gcgaggttac aagatttacg
     6661 ggaaccatcg gagatatcgg aacctccagt ttttatccgc cccatcacat gactatgggt
     6721 gaaggcggag ctgtgtatac agacaacggc cttttaaacc gcattatgcg ctccttccgt
     6781 gactggggac gggactgcgt atgtccttcc ggccgcgaca atatgtgcgg acaccgtttt
     6841 gaccgccagt acggcgagct gcctttaggc tatgaccaca aatatgtata ttctcattta
     6901 ggctacaatt taaaggcaac cgatatgcag gctgccatcg gctgcgccca gttagacaag
     6961 ttcccgtcct ttgtagagcg ccgccgccac aattttgacc gcttaaaggc tgctctggca
     7021 ggcacggagg acaagctgat tcttcctgag gcctgcgagc acgcaagacc cagctggttt
     7081 ggcttcctca tcacctgtaa ggaaggcaca gaccgcaatc aggtggttca gcatattgag
     7141 aaaatgggcg tacagactcg tatgctcttt gcaggaaacc tggtaaagca tccctgcttt
     7201 gacgagatgc gaaagaacgg ggagggcttc cgcgttgtgg gaagtctgga gcatacggac
     7261 cggatcttaa aggatacctt ctgggtaggc gtatatcccg gtatgacgga cgagatgatc
     7321 gactatatgg ctaagaccat ccgtgaagcc attgcctgac aggaacggag gtcgaggaca
     7381 gtatggcaca gacatacgat atgacaaagg tccatgaggc caatttaaag atattaaagg
     7441 agctggaccg gatctgcaga aagtataaga tccggtatgc cctggatgcg ggaaccctga
     7501 tcggcgcagt gcgccacaag ggctttattc cctgggatga tgatgcagat gtggttctga
     7561 tccggagcca gtacgaggcg ttaaaaaagg ttataaaaag ggaacttccc gataccatgg
     7621 agcttttgga gccggacagc taccggggcg gccgggcatt ttatgacttt actccccgta
     7681 ttatttataa aaacagccag gttcatccgg acagcgaccg gatggactat tatgagggaa
     7741 agttaaacca tctctgggta gacctcttta ttctggataa gctgcccgag ggaaagctgg
     7801 cgtctgagat gacccgcttt atccacaagg ccatctacgg cctggctatg gggcaccgcc
     7861 atgatctgga ttatagcaga tacagcctgc ttcacaaaat ttttgtagga ggacttgcca
     7921 cagcaggaaa gctgattccc ctaaagtgga tcatttccat gcagaaatgg gctgccttaa
     7981 aagacagaaa gagcaaagga agccggtggt attacagtaa ttaccagccg gattacctct
     8041 atgtaactct ggaggatgac tggtgcacca agacggagga tgcgcctttt gaagatacgc
     8101 agctgatgat cccaaagggg tatcatcagg tacttacaga ggtttacggc gactatatgc
     8161 agctgcctcc tgaggacaag cgggttccaa cccattccag ccagcagatc cgggtatttg
     8221 actgaatatg ataaaggaga accatccttt gagtgaagat agaagcaatc tgaaaaaaaa
     8281 gattctttcc gtggtcgtgt ctgtttacaa tgaggaacag gcgctgaagg aatgttatac
     8341 agagatgaag agagtgctgg atactctttc ctgggatcat gagctgattt ttgtaaatga
     8401 cggaagctca gatggcagcc tggaggtgct taaagccctg gcttcagagg atcagaaggt
     8461 aaagcttatc agcttttcca gaaatttcgg acatgaggca gccatgatcg cgggtctgga
     8521 ttacagcaca ggcgacggag tggtctgcat ggatgcagat ctgcagcatc ccccggaatg
     8581 tcttccacgg atcatcaggg cctttgagga gggaaacgag gtaatcagta tggtgcgtac
     8641 ccgaaacaaa agcgcaggcc tgatcaaaaa tatcaccagc tcaggatttt actggctgat
     8701 caactgcatt tcggatgttc actttgagcc taatgcctct gacttttttg cggtcagccg
     8761 ttcggtggca gaggttttaa aagggaatta ccgggaaaag gtgcgtttcc tgcggggcta
     8821 tgtccagaac gtgggctttc agaaaactac tctggaatat gaggcaggcg tgagggtggc
     8881 aggagagagc aagtacagct taaaaaagct gtttatcttt tcggttaata ccatcctctg
     8941 cttttccaat atgcccttaa agctgggaat ttacgctgga atcttatcgg gagcagccgg
     9001 gatggcagtt ctggtctata cgctctttac ccgtcaggga gctcccagtg gatatgctac
     9061 cattgtggtg ctgatctgct ttatgttcgc catgatgttt ctgctcatcg gcatcatcgg
     9121 agagtatatc gccatccttt ttacggaact gaaggatcgt ccggtgtata ttgtgaggga
     9181 gacggcgaat atagaacaca attaaagata aatacaaaat accctgaaag ccgttcagcc
     9241 cttcagggta ttttgccgta cgggccagtg agcaggacgc ccggtctaca gctacatcct
     9301 tcgtgaaagg atcattctcc agatgataag gagaccggca aaggctgcca tgatgcagag
     9361 cagaagcttt accatcagga cgcttacaaa gccggacagg aggatcatca catacagaag
     9421 gataaacagg gagtatcccg cataggccca gcttttagta ccgatggcct ggctgcgttc
     9481 gtcaaactcc cggatttcct gctgccttct gcgctcagga tccttcagat aggaacggtt
     9541 tcttaaaatc aataaaactc cggcaatgat cagccccagg ccggtgccgt aatagaagct
     9601 tgtcatgaag ctgcggcttt ccggttccag atagagaatg ggagacgctc cggccgaaga
     9661 agccagagcc gctgtgagag cggccgcacc taaaatcaaa gcagctgctc caagaaagat
     9721 ccgaagcctg catctgcgta ccaaacgggt ctgaccgttg tttttcatag attctctcat
     9781 atgtcgtcct cctcttcaaa aataaaaatt tcttcgatgg tccgtccgaa ataccgggcg
     9841 atcttgtgcg ccagtcccag agaagcgttg tatcttccgt tttctaaaga aatgatcgtc
     9901 tgacgggtaa cgccgactgc ttctgccaga tcctcctgac ggatccgtcc ggtccggcgc
     9961 agttcctgga tcctgttttt catctgtgat cgatcccacc cttcttttta ctgacagtat
    10021 ctttcattaa tggttccact tttacggata gagtgaaatg taaagctaac tgtacaaaat
    10081 aagtatagga aactttacat aaaaagtcaa gttcatttta cattttgtgc ttttttcaga
    10141 tacagtatac aaaatgtttg gagaaatttt ataattgggt cattgacttt ccgacctgta
    10201 agtgtttata atttgaaaag tgacaatatg tcatgtttac cattgactca aggaggcctt
    10261 gatttctatg ccaacccagc ggttttttaa attaaaggaa gaaaaaagac ggctgatttt
    10321 agaagcggcg gcccacgaat tttcccgtgt gccctattcg gcggcttcga tcaatcagat
    10381 cataaaggag gcggatattt cccggggaag cttttataca tattttgaag ataaggatga
    10441 tctgatgcgt tatatgctcc ggggaatgcg ggagcatttc agagaacggg tgatgctgct
    10501 cttgaaagaa atggggagtc cttttgcggt ctgtttacag atggtgcggg agtggatgga
    10561 taagggaaat gaccatatct tttacaagat ccataaaaat ctcctgtcgg atctgagcgt
    10621 ttccaatcaa aatcagatct ttggcttcag aggatttaca ttaaaggatg acccttatga
    10681 gggttttatc aaagatattt atgaacacat ggatcgagag cattatccta tgagcctgga
    10741 ggctcttatt tacggggcgg atgtagccgc tatggtatgt attcgggcga tgagccttta
    10801 ctgccagaag ccgcaggaaa aggaatctat tttacaggca accgaaacag agatgaggat
    10861 tttggaaaaa ggcgtctgca gtctgcaggc ggcaaaatag gatgaaacgg atataaaatc
    10921 gtattagagg agaatgaatc atgaaaaatc agaaaagaaa gacagccggc cgtcatatgg
    10981 ccgcgatcac agctgctatt ttgactatgt catttcccat gaatatcctg gcggcatcta
    11041 accgtccgga tgacaatacc tatcaagctc cggaggcacc ggcccccaat tataataagg
    11101 atatgagccc tgaatttgcc tacacggagg aaaaatgggc ttctctcagg gataacgtga
    11161 tggagtatgg ggagctgaag gatctgatcc atgagtataa ccctacagtc cgcagcaacc
    11221 gttctgccta taaggatcag aagggaaagg acttaaatga cgtatatgag cagtatatga
    11281 acgatatcga tgatatctgg gatcaggcag atgcggctga ggatgatgtt acctgggcgt
    11341 ccatgcggtt ttccgcaggc cttctcacca agcaggcaga caacaattac caggatgcag
    11401 atatggaaaa gatccagtat gaccagcagg aggctgctct ggtatatcag gcccagcaga
    11461 tgatggtatc cttacagcag gcagaagaca atataaagaa tctggaaaat acaaaggctc
    11521 ttcttcagag tcagtatgaa tttaccaggg ctcaggctgc cgcaggcatg gcgacccaga
    11581 tagatgtgct tactgctcag aaaagcgtcc aggatcagga tacagccatc cttgctgccc
    11641 agaagaataa ggaaaatgtc cacagaaatc tctgtctgat gctcggatgg gacgcagacg
    11701 cccagccgga gatccttgca gtaccggagc cggatatgtc ccgtattgat atcttaaacc
    11761 cggatgggga tgccgaggca gcgcttgcca atgactatga tgtaagatat tacgaaaaaa
    11821 aggcaggaaa tctttccagc caggatctga tcgaatccaa tcaggcttcc attcagaatg
    11881 caaaggatac ggctgtgcga tccttaaaaa cgcagtataa tactgtactt acggcaagag
    11941 atgccctgaa tgccgctcag tctcagcttg ctgctgagca atccaaatta aacaaggctt
    12001 ctgccagtct ggcagcagga cagattacaa atcttgagta tcagaccacg ctgaatgctg
    12061 taatgacagc ggaaagcgat gtaaatacga aaaagcttca gctgttagtg gcattagaag
    12121 gctatgactg gaatgtgaag ggccttacca cctccaatta agcaggatga aaaggagacg
    12181 atgaaatgaa aaccacattt ttaaaacgaa cggcagcagc gtccttaagc gtggttctgg
    12241 cagcatctgt gccctttact gtgctggcag atcagacaga tccaaatgtg gcctatgaac
    12301 aggagcagca gcttctctca gacggacatc tggattacgg tgagattgag gaacgtgtaa
    12361 aaaattatta cggaccgatg aaaagcgcct atgatatggc caagggcatg gtagaggatc
    12421 aggctgatat tgctgtaaat gagcgtatta tggcaagtga ccttctgtcc caggccgatg
    12481 tggcagagga tatggcagag gaacagaccg ggatggatca ggcgatttct gcggcaaccg
    12541 ccaaagcact gcgccagtct gccagacaga tgagaaatgc ggcatccatg atgggtcagt
    12601 ccttaaaaaa gacctcatct acggaacgtc aggtagaccg ccaggccaat tccctgatca
    12661 tgaatgtcca gtccatgatg aatcagtatg agcagct
//
LOCUS       DBMDUH010000071        12658 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_2581_length_12658_cov_4.362587, whole genome shotgun sequence.
ACCESSION   DBMDUH010000071 DBMDUH010000000
VERSION     DBMDUH010000071.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 12658)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12658)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12658
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2581_length_12658_cov_4.362587"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="ACZPCG_07400"
     CDS             complement(<1..676)
                     /locus_tag="ACZPCG_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006774273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1284929.1"
                     /translation="MASDENRKKDHAQRDIISRIPNWGWLLISFLAAMLLWKWLSVNP
                     TTARSFPFAPEVLASLGTMIERGVLWQDFSSSMISVVMGFVLGFVTSVPVAFLMAWYR
                     PVRYIVEPWIQFIRNIPPLAYVPLVVISAGVGRRPQVIVIWLATFLIMTVTIYQGVCN
                     VDETLIKAAKVLGAKDSDLFVKVIFPATTPFIITAVRLGVSVALTTLIAAESTGAIAG
                     MGMRIRS"
     gene            911..1933
                     /locus_tag="ACZPCG_07405"
     CDS             911..1933
                     /locus_tag="ACZPCG_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005951233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR1284930.1"
                     /translation="MTLKEIAAEAGVSISTVSRVINKSSKSPAGKEVQDKIWEIVRRT
                     GYTPNHTARELKKGSSTEKEPLSRSLACLFARVEDGAADSFFTPLARSIEKEAFRHGY
                     ILKYTFSAFDIHHPSTFRLIEDNQADGVAVLGRCDKELLKFLKQYFKYVVYAGLNSLD
                     AKYDQIICDGGQAAYAAVSRLIELGHKKIAYIGETDKEERYNGYCRALEHAGIPILKG
                     YISNVKLSSEGGYQGANILFDRDCDATAWFCSNDMTAIGAMRAVKEHGLQIPEDISII
                     SIDDIDTAQYLTPMLTTVHIPIEEMGKMAAKTLIDRIEGGHTLPMKISFPFYIAGRES
                     CREYRG"
     gene            complement(1962..2702)
                     /locus_tag="ACZPCG_07410"
     CDS             complement(1962..2702)
                     /locus_tag="ACZPCG_07410"
                     /inference="COORDINATES: protein motif:HMM:NF024950.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR1284931.1"
                     /translation="MFEGKTAVVTGAGKGLGQAMAVALARAGAKLIGVDIGDMTETSR
                     IIEENGGTFTAVNADLSKTGQIPELAKKLEGIYGKVDILVNNAGVIYRSPAEEYPEDG
                     FDFVMNLNVKALFLLTREIGGCMKQRGWGRIINVCSIQSMQGGNGVSAYAASKHAVAG
                     ITRAFANEWGKYGITVNGIAPGYMVTDNTDKLRAQKEKAAAITSRIPVGRWGQPSDLM
                     GPVVFLASEEAAYVNGHLLVVDGGYMNA"
     gene            complement(2724..3200)
                     /locus_tag="ACZPCG_07415"
     CDS             complement(2724..3200)
                     /locus_tag="ACZPCG_07415"
                     /inference="COORDINATES: protein motif:HMM:NF017936.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR1284932.1"
                     /translation="MMEYTADEIIKRLGMQPHREGGWYRFVWKNEQTVAEGALGDRYK
                     GERSVCSLIYYLLKEEEVSRWHQLKSPEIWTWHCGDSLVMTLGGEKEEPDGNTQVRIG
                     DKLDQGDTFQFVVPGGIWQTTQLEQESERKHGFALVSCIVSPAFTPEDCYLPDPLF"
     gene            complement(3221..4276)
                     /gene="mtnA"
                     /locus_tag="ACZPCG_07420"
     CDS             complement(3221..4276)
                     /gene="mtnA"
                     /locus_tag="ACZPCG_07420"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HIR1284933.1"
                     /translation="MKMDREIALSVKYNDGRLFFLNQTLLPEKEEYVEITNETEAWTA
                     IKRLVLRGAPAIGVGAAYALSVCADHLETEEVSSFCKEVHRIKEYLNSSRPTAVNLSW
                     ALNRMENRMLSGNKESTAELKAVLREEAEAIYNEDVMLCRKIGEAGQEIMKDGMGILT
                     HCNAGALATSRYGTALSPIYVAQERGLNVRVFADETRPLLQGSRLTAYELTRAGVDTT
                     VITDSMAAVVMKKGWVDAVIVGADRIAANGDAANKIGTYGVALLAKAHNIPFYVACPY
                     STVDMNTPSGEDIVIEERAAEEVLCGMGKQTGPKEAKVFNPAFDVTPHELIAGIITEK
                     GILRAPYNESLRKLDCR"
     gene            complement(4263..5045)
                     /locus_tag="ACZPCG_07425"
     CDS             complement(4263..5045)
                     /locus_tag="ACZPCG_07425"
                     /inference="COORDINATES: protein motif:HMM:NF012669.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HIR1284934.1"
                     /translation="MLAPERRNQILNLVIQKKSVLVKDLSMMFDVTGETIRKDLAVLE
                     KEGKILKTYGGAYVCDGVQNEIPITLRESLYTDEKKAIGAVCASLVQNGDTVSLDGST
                     TCLHIAKNLIDRTDVTVLTNSLRIADVFSDCPNIRLILSGGELNQEIQCFVNHGSEEM
                     LSRYFVDKCFVSCRGIHMQTGITDGSESNGRIRRIMLEHAKQKYLVVDSTKIDLINFY
                     RIGTFDLVDAMVIDRLDSTNWKNFIYEKGIRLIEAGKENENG"
     gene            complement(5053..5715)
                     /locus_tag="ACZPCG_07430"
     CDS             complement(5053..5715)
                     /locus_tag="ACZPCG_07430"
                     /inference="COORDINATES: protein motif:HMM:NF012804.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HIR1284935.1"
                     /translation="MSKDNLNIKQEICEIGKLMYDRGYVVSNDGNISVRVSENEIIIT
                     PSGVSKGRMTPDMMVTVDLDGNILEGKRYPSSEMKMHLAVYKERSDINSVIHAHPPVS
                     TAFAVCRKPLDRMYMPETVLNMGTIPVTEYALQSTQAVPDSLKPFVNGNGALIANHGV
                     IVWAQDLWGAFHRMETVEHTAKIYMNIQMIGNGCELSEKELEELYSLREYYKKLAEER
                     KE"
     gene            complement(5743..6558)
                     /locus_tag="ACZPCG_07435"
     CDS             complement(5743..6558)
                     /locus_tag="ACZPCG_07435"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013280918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HIR1284936.1"
                     /translation="MLEKQINETVEYIRSKIGTVPEAAIVLGSGLGGFADQLEDAVRI
                     PYEEIPGFVRSTAPGHKGQIVCGKKSGKVVLCMQGRFHYYEGYTMQQVTYPVRVIKAL
                     GISKLILTNSCGGLKSEFEPGDLMLITDHINFMGANPLIGPNEDMFGCRFPDMTKAYN
                     KELAEKARKCAGELSIDLKEGTYVGYGGPSFETPAEIRMFQMLGGSAVGMSTVPEVIA
                     ASHCGLNVLALSCITNLAAGILDQPLSAEEVIEVAGKAGKKFTGLLEKIVEII"
     gene            complement(6562..7461)
                     /locus_tag="ACZPCG_07440"
     CDS             complement(6562..7461)
                     /locus_tag="ACZPCG_07440"
                     /inference="COORDINATES: protein motif:HMM:NF014684.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1284937.1"
                     /translation="MSGDMMVVIQSLITTTLRMSIPVILASLAGVFSARAGIVAMGLE
                     GMMLSGAFSAAYVTYITGNPFLGILGGILAGVVLSLVHGVLVIKFRLDQVIGGIGLNL
                     FATGLTTVFMQLVWGNRGKSEMLNTIPSIKVPVLGKISPLLLIALIAVGLAGYVLYKT
                     VFGLHLRMVGENPTAAKTLGIYVNRIRFQSLIIVGILSGLSGAYLSIDHLNMFVRDMV
                     AGRGFIALSIDILGRYTPGGILAGSLFFGFVDALQLVIQSDKIPGQLLQMLPYLVTLL
                     AVVLAVKDVRPPASMGKVIEEKE"
     gene            complement(7451..8506)
                     /locus_tag="ACZPCG_07445"
     CDS             complement(7451..8506)
                     /locus_tag="ACZPCG_07445"
                     /inference="COORDINATES: protein motif:HMM:NF014684.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1284938.1"
                     /translation="MKKRIEKMIPTVLSLAIALFFGSFLFMAVGYNPVSVYSEIIYGA
                     VGTHGAVLNSLVQATPLLFTGLAFVIAIRGGIANIGGEGQLYAGALGSVLAGIFLKDI
                     PAYIHIPLSLLSGIVFAGCWGSLIAVLKVYFGAQEIITAIMLNYIMQYFVSFLVNYPL
                     KEEGAISQTVEIADTAKLPLLSAQYQLSSGILLAVFIAVAMTFIYKKTLMGYEMSITG
                     KCKAAARTAGINVDKTMIRTMFISGATAGLCGSVMILGSAYRLIDSFSSGYGFDGIAV
                     ASLANGSFMGVLLSGTFFGALRAGAMYVNRVAKIPYDFAIVIQALIIVLIATPYMWHV
                     LGSKLKWKKKGEANYVR"
     gene            complement(8503..10017)
                     /locus_tag="ACZPCG_07450"
     CDS             complement(8503..10017)
                     /locus_tag="ACZPCG_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004069751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1284939.1"
                     /translation="MEVVRLENITKQYPGVLALDHANLEICSGEIHALLGENGAGKTT
                     LMKVLYGMTVPDEGKIYINQKQVSFSSPIEAISAGIGMVHQHFMQAEMLTVLENVIAG
                     AEPSAKGKIKYAKARRGVQEMIDRFSFDINLDSKIEDLSVGERQRVEILKVLYRGGKI
                     LIFDEPTAVLTPLETEAFFTVLRKFKEQGKSIVIITHKLQEVMEIADRITVMRDGVIT
                     GHCMPQDVTINDLASMMVKREIKVNEHMRAETVSPEVYFEVKDLLLTESEKVILDHIS
                     LKIHKGEILGIAGVEGNGQTELIRCLTGLQKPDTMELIVDGEAVTGNASDFIRQGIGH
                     VPEDRLGTAVVENDSISTNLMLGYHFKERFRKGRWMDYNKADTFGNECIETYSIKAPS
                     SKTTIAEMSGGNQQKVVIARVFSENPKVVICAQPTRGVDIGSIEYIHRRMREFVGTEH
                     AMLLISADLEEVKAMSDRIAVIFKGKIVAEGSTDQFDDTQLGLYMTGAGTEEER"
     gene            complement(10091..11191)
                     /locus_tag="ACZPCG_07455"
     CDS             complement(10091..11191)
                     /locus_tag="ACZPCG_07455"
                     /inference="COORDINATES: protein motif:HMM:NF014647.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family lipoprotein"
                     /protein_id="HIR1284940.1"
                     /translation="MRKIVAKVVLAGMVMSVTGCGGKTITHETEATAVSGAEESGAEE
                     KVKVGFIVGTGGLGDQGFNDIAYEGLKRAEEELGIELEYAEPQSVSDFEPLISQFAQD
                     GSYDLIISLDQQSQSALEKIAELFPEQKFSAVDMNIEADNVKVIRKDFSQLAFLPGYF
                     AGLLTTDTSVDKVNEEACVGIMIGVDNPNMQNGVLGYTAGARLANPDTEVLTGIVGSY
                     GDPAKGKDTAKVMYDKGADIVMNFAGSSGLGLTNQAKESERLVIGGTSNVNATAPDVI
                     AASALEQLSDRVYNDVKAVIDGTWESGIEMGVIANGGVDIAFEGTDVAVPEEIIEKID
                     HVRTLIKDGKLTLPSSVEEIDGWLEEAAGVLK"
     gene            complement(11517..11948)
                     /locus_tag="ACZPCG_07460"
     CDS             complement(11517..11948)
                     /locus_tag="ACZPCG_07460"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1284941.1"
                     /translation="MKTRIHSTLPQEARRIREEVFVREQGFENEFDETDQSASHLILY
                     DGEMPVAVCRFFPGDQPGDYMVGRIAVIKEYRGRNLGARLLKEAEKEIRTLGGRRILL
                     HAQCRAAGFYEKQGYASQGEVDFDEDCPHIWMGKEIAERKE"
     gene            complement(11956..12441)
                     /locus_tag="ACZPCG_07465"
     CDS             complement(11956..12441)
                     /locus_tag="ACZPCG_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284942.1"
                     /translation="MGFFGKLFKGPEVDMEKSNANAKKMRLLFDQAVEGGSGYRLIFG
                     YTEDVSRFNYGFVHGSKTKIGNLIVGWNEANQTIVVVPTVPDLSGCGDPTYYRRSEIL
                     KAYRNKYPTDAFIIYPDKKGYIGINAYDWLEDEKLYVYVSQADELAAFTDFFMNRFAK
                     K"
     gene            complement(12464..>12658)
                     /locus_tag="ACZPCG_07470"
     CDS             complement(12464..>12658)
                     /locus_tag="ACZPCG_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR1284943.1"
                     /translation="NFGIKANVERIHLFTNLSLTVMVIRRAIPDTLPFFVPEAADETV
                     DFQLNAIVDALEKLKEETNI"
BASE COUNT         3340 a         3397 c         2221 g         3700 t
ORIGIN      
        1 aagaacggat cctcattccc atacctgcga tcgcgcctgt agactcagcg gcgatcaggg
       61 ttgtaagagc tacggatacg cccaggcgga ctgcggtaat gataaatgga gtcgtagccg
      121 ggaaaataac ctttacaaac agatcgctgt cctttgcgcc cagcaccttt gcagctttga
      181 tcaaggtctc gtctacgttg cacacaccct gataaatcgt cacggtcata ataaggaagg
      241 ttgccagcca aatcacgatg acctgaggcc gtcttcctac tccggcagaa ataactacca
      301 gcggaacata ggccagaggc gggatgttac ggatgaactg gatccacggc tctacaatgt
      361 agcgcaccgg ccggtaccag gccatgagaa aggctaccgg aaccgatgta acaaagccaa
      421 ggacaaagcc catcaccacg gaaatcatac tgctgctgaa atcctgccac agtacgcctc
      481 gctcgatcat ggtacccagg gacgccagca cctccggcgc aaagggaaag ctgcgggccg
      541 tagtcggatt cacagacagc catttccaca gaagcatcgc tgcaagaaag gaaataagca
      601 gccagcccca gttggggata cggctgatga tatcccgctg cgcatgatct ttcttcctgt
      661 tttcatcaga agccatgatt tttcctcctg ttttttattt ttggctctta tttttgtcgt
      721 ctttgccaaa tcatttctgt ttcttatggt ttcagtatat catgttgtac aaaatagtca
      781 ctgcctattt acccaaacgt ttgccttgtt ttctcttatt ttttctttca tttttgcgtt
      841 tggctctatt tttttgtgtt tttatcatgt atacttacat catagcatca gtgggtaagg
      901 aggaaatcgc atgacactga aggaaatcgc tgccgaggca ggcgtatcca tttccaccgt
      961 atcgcgggta atcaataaaa gcagcaaatc tccggccggg aaagaggttc aggacaaaat
     1021 ctgggaaatc gtccggcgca ccggctatac cccaaatcat acagccaggg aactaaaaaa
     1081 aggctcttcc actgagaaag aacccttatc ccgctcgctt gcctgcctgt ttgccagagt
     1141 agaggatgga gccgctgaca gcttctttac tcctcttgca aggagcatcg aaaaagaggc
     1201 gttccgccac ggctatatat taaaatatac cttttccgcc tttgatatcc atcatcccag
     1261 caccttccgc ctcattgaag acaatcaggc agacggcgta gcggtcctgg gacggtgcga
     1321 taaagagctg ttaaagtttt taaaacagta ttttaagtat gttgtgtatg ccggactcaa
     1381 cagcctggac gccaaatatg accagatcat ctgcgacggc ggtcaggccg cttatgctgc
     1441 agtcagccgt ctcattgaac ttggacataa aaaaatcgcc tatatcggcg aaacagataa
     1501 agaagagcgc tataacggat actgccgggc tctcgaacat gcaggaatcc ctattttaaa
     1561 gggatatatc agcaatgtaa agctctcctc cgaaggagga taccagggag ccaatatcct
     1621 gttcgacaga gactgcgatg ccacagcctg gttctgctct aacgatatga ccgcgattgg
     1681 ggccatgcgt gccgtaaagg agcacggact tcagataccg gaggatatct ccattatcag
     1741 tatcgatgat attgatacgg cccagtatct gacccccatg ctcaccacgg tccacatccc
     1801 catcgaagag atgggaaaaa tggccgcaaa aacactcatc gaccgaatcg aaggcggcca
     1861 taccctgccg atgaaaatca gctttccgtt ttatattgca ggaagggaaa gctgcaggga
     1921 gtatcggggc tgatcctcga tgttccctgc agctttcaaa tttatgcgtt catataacca
     1981 ccgtctacta ctaaaagatg accatttaca taagcagctt cctcagaagc cagaaataca
     2041 accggcccca tcaaatcgga tggctgtccc cagcgtccca ccgggattct ggatgtgatc
     2101 gctgccgcct tctccttctg ggcccgcagt ttatctgtat tatccgtcac catatagccc
     2161 ggagcaattc cattgacagt aattccgtat tttccccatt catttgcaaa tgccctggta
     2221 ataccggcta ccgcatgttt gctcgctgca taggcagata cgccgtttcc tccctgcata
     2281 gactgaatgg agcatacatt gataattctg ccccagccgc gctgcttcat gcagccgccg
     2341 atttccctgg taagaagaaa caaagctttc acattcagat tcattacaaa atcaaaacca
     2401 tcttccggat attcctccgc cggacttctg taaatcacgc ctgcattgtt gaccagaata
     2461 tcaacttttc cataaattcc ttccagcttt tttgccagct ccggaatctg gccggttttg
     2521 cttaaatctg cgttcactgc tgtaaatgtt ccaccatttt cctctatgat acgagatgtt
     2581 tccgtcatat ctccaatatc aacaccgata agcttcgcgc ctgcacgcgc caatgcaact
     2641 gccattgcct gtcccagacc ttttccggct cctgtgacaa ctgctgtttt tccttcaaac
     2701 atatagactc atcctccgct ttttcaaaat aagggatccg gcaaataaca gtcttccggc
     2761 gtaaaagccg gagaaacaat gcaggacaca agagcaaatc catgttttct ttctgactcc
     2821 tgctccagct gggtggtttg ccagataccg cccggtacta caaactggaa tgtatctccc
     2881 tgatccagtt tatctccaat ccttacctgg gtgtttccgt ccggttcttc cttctctcca
     2941 cccaatgtca taaccagaga atctccacaa tgccaggtcc agatttcagg agactttagc
     3001 tgatgccatc ttgatacttc ctcttccttt aaaagataat aaatcaggct acaaacagag
     3061 cgttcccctt tatatctatc gcccagggct ccctccgcta ctgtctgctc attcttccag
     3121 acaaagcgat accacccgcc ctctctgtgc ggctgcattc ccagacgttt aataatctca
     3181 tctgcagtat actccatcat ttaagcttct cctgttcttt ttatctgcaa tcaagctttc
     3241 tgaggctttc attgtatgga gctctcaaaa ttcccttttc cgtaataatt cccgcaatca
     3301 gctcatgggg agtaacatca aaggccggat taaacacttt tgcctctttc ggtccagttt
     3361 gctttcccat accacacaat acttcctcag cagctctctc ctcaattaca atatcctctc
     3421 ctgaaggcgt attcatatcc actgtagaat atgggcacgc aacataaaat ggaatattat
     3481 gagctttggc aagaagagct acgccatagg ttccaatttt attggccgca tcaccattgg
     3541 cagcgatacg gtcggctcct acaatcacag catctaccca tccttttttc ataacaactg
     3601 cagccatact gtctgtaatc acggttgtat caacacctgc tcttgtaagt tcatatgcgg
     3661 tcagtctgct tccctgcagc agcggacggg tttcatctgc aaatacgcgt acattaagcc
     3721 ctctctcctg cgccacatag atgggagaaa gagcagttcc ataacgtgaa gttgccaacg
     3781 ctcccgcatt gcagtgggtc agaatcccca tgccgtcctt cataatttcc tgacctgctt
     3841 caccaatttt acggcacagc attacatctt cattatagat tgcttcagcc tcttctctta
     3901 aaaccgcctt taactcggct gtgctttctt tatttcctga aagcatccgg ttctccatcc
     3961 ggttaagagc ccatgacaag tttacagccg taggcctgga gctgtttaaa tactccttaa
     4021 tccgatgtac ttccttacaa aaggaggaaa cctcctctgt ttccaaatgg tctgcgcaga
     4081 cgcttaatgc atatgccgct cctactccga ttgccggtgc cccccttaac accagccgtt
     4141 tgattgccgt ccacgcttcc gtttcatttg ttatttccac atattcttcc ttttccggaa
     4201 gcagcgtctg attcagaaag aacagccgtc catcattata ttttactgat aatgcaatct
     4261 ctctatccat tttcattttc cttcccggcc tctatcaacc gaatcccctt ctcataaata
     4321 aagtttttcc aattggttga atccagtcta tcgattacca tagcatcaac cagatcaaag
     4381 gtaccaatcc gatagaaatt gatcaaatca atctttgtac tgtctacaac caaatatttt
     4441 tgctttgcat gctccagcat aatacggcgg attcttccat tactttcact cccatctgta
     4501 atgccggtct gcatatgaat ccccctgcag gaaacaaaac atttatctac aaaatatctg
     4561 gataacattt cttcgcttcc atgatttaca aaacactgga tttcctgatt cagctcacct
     4621 ccagataaaa tcaaccgaat atttgggcaa tctgaaaata catctgcaat tctgagtgag
     4681 tttgtcaata cagtcacatc tgtgcggtct ataagatttt tagcaatatg aaggcatgtt
     4741 gtgcttccat ccagactgac tgtatctcca ttctgaacca gagaggcaca gacagcaccg
     4801 atcgcctttt tttcatctgt atacaggctt tccctcaatg taatcggtat ctcattttga
     4861 actccgtcgc agacatacgc tccgccgtac gtctttaata ttttgccttc tttttctaaa
     4921 actgccaaat ccttccggat ggtctcacct gttacgtcaa acatcatact cagatctttg
     4981 accaatacgc tttttttctg gattaccaaa tttaatatct gatttcttct ttcaggagct
     5041 aacatattta ccttactctt ttctttcttc tgctaatttt ttgtaatatt cccttaaact
     5101 ataaagttct tccagctcct tttctgacag ttcacagcca ttccctatca tctgtatgtt
     5161 catatagatt ttagcggtat gctctacagt ttccatccgg tgaaatgccc cccacaaatc
     5221 ctgggcccat acaattactc catgattcgc aatcaaagcg ccgtttccgt ttacaaacgg
     5281 cttcagcgag tccggaactg cctgggtgga ttgaagtgca tattctgtga ctggtatcgt
     5341 tcccatattt aacaccgttt ccggcatata catacgatcc aggggcttcc ggcaaacagc
     5401 aaaggctgta gatactggcg gatgagcgtg aatcactgaa tttatatccg atctttcttt
     5461 atatacagcc aaatgcatct tcatctcaga ggacggatag cgttttcctt ccagaatatt
     5521 gccatccaag tcaaccgtaa ccatcatatc aggcgtcatt cgtcccttgc tgacacctga
     5581 aggagtaata atgatttcat tctcactgac tctgacagaa atatttccat cattgcttac
     5641 cacatatccc ctgtcataca tcagttttcc gatttcacat atttcctgct ttatatttaa
     5701 gttgtcttta ctcattttct gcaacacgct tctttcacta ttctatatta tttctactat
     5761 tttttccagc aatccggtga atttttttcc cgcttttcct gctacttcga ttacttcctc
     5821 ggctgaaaga ggctgatcca aaataccggc tgccagattt gtaatacagg ataatgccag
     5881 gacatttaag ccacaatgtg acgctgcaat cacctcagga actgtggaca ttcctacagc
     5941 gctgcctccc agcatctgga acatacggat ttctgccggt gtttcaaagc ttggcccgcc
     6001 gtatccaaca tatgtaccct cttttaaatc aatggaaagc tctccggcac actttctggc
     6061 cttttccgcc agttctttat tgtatgcctt tgtcatatcc ggaaatctgc atccgaacat
     6121 atcttcattg ggtccaatca aaggatttgc ccccataaag ttaatatggt ctgtaatcag
     6181 catcagatcg cccggttcaa attcagactt taatcctccg caggaatttg tcaaaatcaa
     6241 ttttgatatg cccagcgctt ttatcacgcg taccggatag gttacctgct gcatggtgta
     6301 tccttcatag taatgaaatc tgccctgcat acagagcaca acttttccgc tcttctttcc
     6361 gcagacaatc tgccccttat ggcctggggc cgtagatctt acaaagcccg gaatctcttc
     6421 atacggaatg cgcaccgcgt cctctagctg atccgcaaaa cctcccagac ctgatcctaa
     6481 aacaatcgcc gcctccggca cggttcctat tttgcttctg atgtattcta ccgtctcatt
     6541 aatctgcttt tccaacatat attactcctt ttcttcaatt acctttccca tactcgcagg
     6601 aggccttaca tcttttacag ccagcactac agccagaagc gttaccagat atggaagcat
     6661 ctggagaagc tgtcccggta tcttatcact ctgtatcaca agctgcaggg cgtccacaaa
     6721 tccgaaaaac agacttccag ctaaaattcc gcccggtgta taccgcccca aaatatcaat
     6781 ggataatgca ataaaccctc tgccggcaac catatcacgt acaaacatat tcaaatgatc
     6841 gatggataaa tatgcacctg acaatcccga aagaattcct acaatgataa gactctgaaa
     6901 ccggatacga tttacataaa ttcccagagt tttagccgcc gtcggattct cacccaccat
     6961 acgcagatgg agaccaaaca cagtcttata aaggacgtaa ccggccaaac ctacagcaat
     7021 cagagcaatt agcagcaaag gtgaaatttt tcccaataca ggaaccttaa tggatggaat
     7081 tgtattcaac atttcggatt ttcctctgtt cccccacaca agctgcataa aaacggttgt
     7141 aagtcctgta gcaaacagat tcagaccgat tcctccaatg acctgatcca agcggaattt
     7201 tataactaaa actccgtgaa caagagatag cacgacaccc gccagtattc cacccagtat
     7261 tccaagaaat gggtttcctg taatatacgt cacatatgct gccgaaaagg caccgctcaa
     7321 catcattccc tcaagcccca tggcaacaat acctgctctt gctgaaaaca cgcctgccag
     7381 agaggccaaa atgacaggaa tactcatacg taaggttgta gttatcaaag attgaataac
     7441 caccatcata tcacctgaca tagtttgctt ctcccttctt tttccatttt agcttactgc
     7501 cgagaacatg ccacatataa ggcgtagcaa tcagcacaat aatcaatgcc tgaattacga
     7561 tcgcaaaatc atagggaatt tttgcaacac ggtttacata catggctcct gctctcaatg
     7621 cgccaaaaaa cgttcctgac agcaagacac ccataaagct tccgttagcc agagatgcta
     7681 ctgcaattcc atcaaatcca tatccggatg aaaaactatc aatcagacga tatgcacttc
     7741 ctaagatcat gacagaaccg cataatccag cagtcgctcc gctgataaac atggttctta
     7801 tcatggtttt atccacgtta attccggcgg ttcttgcagc tgccttacat ttacctgtaa
     7861 tagacatttc atatcccatc aaagtttttt tataaataaa cgtcatagct acagcaatga
     7921 aaactgccaa taaaatcccg gaagaaagct gatactgtgc agaaagaagg ggaagcttcg
     7981 ctgtatctgc aatttcaacg gtctgggaaa ttgctccttc ctctttcaga ggataattta
     8041 ccagaaaact cacaaaatac tgcataatat aattcagcat aatagcagta ataatttcct
     8101 gagccccgaa ataaactttc aaaaccgcaa tcaggctccc ccagcatcct gcgaatacaa
     8161 tacctgacaa aagactaaga ggaatatgaa tatatgccgg tatgtccttt aaaaaaatcc
     8221 ccgcaagaac agagcccaat gcacctgcat acagctgtcc ctctcctccg atattggcta
     8281 taccgcctct aatcgcaatc acaaatgcca gtcccgtaaa cagaagcggc gtagcctgta
     8341 ccagagagtt taaaaccgca ccatgagttc ccactgcccc ataaataatc tcgctgtata
     8401 cactaacagg attatatcct actgccataa agagaaacga accgaaaaac agggcaattg
     8461 ccagcgaaag aactgtagga atcatttttt ctattctttt tttcatctct cttcctccgt
     8521 tccggcgcct gtcatataca atccaagctg tgtatcatca aattgatctg ttgatccctc
     8581 tgccacaatc tttcctttaa aaatcactgc aatcctgtcg ctcattgctt ttacttcctc
     8641 cagatcggca gaaatcagca gcatcgcatg ctctgtacct acgaactctc tcattcttcg
     8701 atgaatatat tctatcgatc caatatccac gccgcgggta ggctgtgcgc agataactac
     8761 cttgggattt tcgctgaata cccttgcaat aactaccttc tgctggtttc ctcccgacat
     8821 ttcagcaatg gttgtctttg aggacggcgc tttaattgaa tacgtttcaa tgcactcatt
     8881 cccaaaggtg tccgctttat tatagtccat ccatctgcct tttctaaacc gctctttaaa
     8941 atggtatcct agcattaaat tcgtgctgat tgaatcattt tcgactactg ccgttcccag
     9001 ccgatcctcc ggcacatggc cgatcccctg acggataaaa tcacttgcat ttccggtcac
     9061 ggcttcccca tcgacaatca gctccatggt gtccggcttt tgaaggcctg taagacagcg
     9121 tatcagttcc gtctggccat ttccctctac tcccgctatc ccaagaattt cccccttgtg
     9181 gattttcaga ctgatgtgat ccagtatcac cttttctgat tctgtgagca gaagatcttt
     9241 gacttcaaaa tatacctccg gacttacggt ctctgccctc atatgctcgt ttacctttat
     9301 ttcccgcttt accatcatag aggccaggtc attgatcgtt acgtcctgag gcatgcaatg
     9361 tcctgtaata actccatcac gcataaccgt aatccggtcc gcaatttcca ttacctcctg
     9421 taatttgtga gtgataatta caatactttt tccctgttct ttaaatttcc ttaaaacagt
     9481 aaaaaatgct tctgtttcca gaggagttaa aacagcggtc ggttcatcaa aaatcagtat
     9541 cttccctccg cggtataata cctttagtat ttctaccctc tgacgttctc caaccgaaag
     9601 atcctcaatc ttgctgtcca gattgatatc aaaggaaaaa cggtcgatca tttcctgtac
     9661 acccctgcgt gcctttgcat attttatttt tccctttgca gaaggctccg ctcctgcaat
     9721 cacattttcc agtacagtaa gcatttcggc ctgcataaaa tgctgatgca ccataccaat
     9781 tccagctgaa atcgcttcaa ttggcgagga gaaacttacc tgcttttgat tgatgtatat
     9841 tttgccttca tccggaactg tcattccata aagcactttc ataagagttg tttttcctgc
     9901 cccattctct cctaaaaggg catgtatttc tccggagcaa atctccagat ttgcatggtc
     9961 aagagccagc actcccggat actgttttgt aatgttttct aatcgaacaa cttccacttt
    10021 cagattcacc tcgccaattc tatcttttgc aacaattcag tgatattctg ccgtacttac
    10081 ctgccagtga ctactttaat accccagctg cttcctctaa ccatccgtca atctcctcta
    10141 cagaagatgg aagtgtgagt tttccatctt tgatcaacgt tcttacatga tcgattttct
    10201 ctataatctc ttccggaaca gcaacatctg taccttcaaa cgcaatgtct actccaccgt
    10261 ttgcaattac acccatctca attcccgact cccatgttcc gtcaatcaca gcctttacat
    10321 cattatatac tctgtcactt aactgctcca gggctgacgc tgctattaca tccggagccg
    10381 ttgcattgac attggaggtt ccgccaatca ccagacgttc tgactctttt gcctggtttg
    10441 taaggcccag accggaactt cctgcaaaat tcataacaat atccgcacct ttatcataca
    10501 taacctttgc agtatctttt cccttggccg gatctccata agatcccacg ataccggtta
    10561 atacttctgt atccggattt gcaagccttg ctcctgctgt ataacctaaa acaccattct
    10621 gcatattagg attgtcaaca ccaatcataa ttccaacaca tgcttcttca tttaccttat
    10681 caacagacgt atccgttgtc aaaagacctg caaaatatcc aggaagaaaa gccagctgag
    10741 aaaaatcttt tctgattacc tttacattat cagcttcaat attcatatca actgccgaaa
    10801 acttctgttc agggaataat tcagcaatct tctctaatgc agactgagac tgctgatcca
    10861 gtgatataat cagatcatag cttccgtcct gggcaaactg actgatcaac ggctcaaagt
    10921 ctgaaacgga ctgaggctct gcatattcaa gttcaattcc aagctcctct tccgcccgct
    10981 ttaatccttc atatgcgata tcattaaatc cctggtctcc cagtccgcct gttcctacga
    11041 taaaacctac cttcactttt tcctcagctc cgctttcctc agccccggaa actgcggttg
    11101 cctccgtttc atgggtaatg gtttttccac cgcatcctgt aacgctcatt accattcctg
    11161 ccagtacaac ttttgctacg atctttctca tttgttttct cctttcacat ccaatacatt
    11221 ccaacttttc tgatgtttgc agttatatta taccatgatt tccctttatt tcaactgtat
    11281 tttatttgtt tttattattt tatacttgtt tttaatttgt ttcttgttga ttttgtcaaa
    11341 atgttcaata taatacgatt tatcaatatt ttttggtaat aatattttat atataatttt
    11401 ttctaatata tagaaagata aacatttata tctttttagc tattttttat atagacaaaa
    11461 agcgccgatt cacaaaatga accaacgctt ctttcagact cttacactat aaaaccttat
    11521 tcttttctct ctgcaatctc cttccccatc catatatgag gacagtcttc atcaaaatca
    11581 acttccccct ggctggcata cccctgcttt tcataaaagc ccgctgccct gcactgcgca
    11641 tggagaagga tccgtctgcc tccgagtgtc cgtatctctt tttcagcctc ctttaacaga
    11701 cgggctccca gattccggcc ccggtactcc tttataaccg caatccggcc gaccatataa
    11761 tctccgggct gatcaccagg gaagaaacgg cataccgcga ccggcatttc accatcatac
    11821 agaatcagat gagaagcgct ctggtccgtt tcatcaaatt cattctcaaa gccctgttcc
    11881 cttacaaaga cctcctcccg aatccttctt gcctcctggg gcagtgtgct gtgaatccgt
    11941 gttttcatag tttcctcact tttttgcaaa acggttcata aaaaagtctg taaacgcagc
    12001 cagttcatcg gcctgcgata cataaacata cagtttttca tcttccagcc agtcataggc
    12061 attgatgccg atatagccct ttttgtccgg gtaaatgata aaggcatcgg tgggatactt
    12121 attcctgtat gcttttaaaa tctcagaacg gcggtagtat gtcggatcgc cgcagccgga
    12181 aagatcggga accgtcggaa cgaccacgat tgtctggttt gcctcattcc agcccacaat
    12241 taaatttccg atctttgttt tgctcccgtg aacgaatcca taattaaagc ggcttacatc
    12301 ttcggtatat ccaaaaatca acctgtaacc gcttcctccc tctacggcct ggtcaaacag
    12361 cagacgcatt ttctttgcat ttgcattgct cttttccatg tcaacctcag ggcctttaaa
    12421 cagttttcca aaaaatccca taatcttttt cctcctgaat cttttatatg ttcgtttcct
    12481 cttttaattt ttccagggca tccacaatgg cattcagctg aaaatcaacc gtttcatccg
    12541 cagcttccgg aacaaagaaa ggaagtgtat cgggaatggc tctgcgtata accatcacgg
    12601 tcaagctcag atttgtaaac agatggatcc tttctacatt cgcctttatc ccaaagtt
//
LOCUS       DBMDUH010000072        12636 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_2587_length_12636_cov_6.583726, whole genome shotgun sequence.
ACCESSION   DBMDUH010000072 DBMDUH010000000
VERSION     DBMDUH010000072.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 12636)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12636)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12636
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2587_length_12636_cov_6.583726"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(234..321)
                     /locus_tag="ACZPCG_07475"
     tRNA            complement(234..321)
                     /locus_tag="ACZPCG_07475"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(285..287),aa:Ser,seq:gct)
     gene            complement(516..3026)
                     /gene="gyrA"
                     /locus_tag="ACZPCG_07480"
     CDS             complement(516..3026)
                     /gene="gyrA"
                     /locus_tag="ACZPCG_07480"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIR1284944.1"
                     /translation="MEPNVFDKVHDVDLKKTMEQSYIDYAMSVISARALPDARDGLKP
                     VQRRVLFSMIELNNGPDKPHRKCARIVGDTMGKYHPHGDSSIYGALVNMAQDWSTRYP
                     LVDGHGNFGSVDGDGAAAMRYTEARLSRISMEMLADINKDTVDFAPNFDETEKEPMVL
                     PARFPNLLVNGTSGIAVGMATNIPPHNLREVIGAVSLMIDNRVNEDRDTSIEELMEVI
                     KGPDFPTGATILGRRGIEEAYRTGRAKIKVRAVTNIETMANGKSRIIVTELPYMVNKA
                     RLIEKIADLVKEKKIDGITYIGDESNREGMRINIELRRDVNANVILNQLLKHTQLQDT
                     FGVIMLALVNNQPKILNLHQMLEVYISHQEEVVRRRTQYDLNKAEERAHILQGLLIAL
                     DHIDEVIRIIRGSANVAEAKTQLMERFGLSDAQSQAIVDMRLRALTGLEREKLENEFK
                     ELQAKIAQLKAILADEKKLLMVIREEINIIAAKYGDDRRTAIGFDDDMSMEDLIPDED
                     TVVAMTHLGYIKRMDADNFKSQNRGGKGIKGMQTIEEDYIEDLLMTTNHHHMMFFTNT
                     GRVYRLKAYEIPEAGRTARGTAIVNLLQLQPGEKITAIIPMREFNEDQYLFMATKEGM
                     VKKTPMVEYGNVRKNGLQAIVLKEGDELIEVKATDDKQDIFLVTKKGMCIRFKEKDVR
                     TTGRVSMGVIGMRFDEDDEVIGMQMESQGEELLVVSENGMGKRTPIEEFKPQFRGGKG
                     VLCYKITEKTGNLVGAKLVNDGREIMLITTEGIIIRMSVDDISVIGRNTSGVKLMSID
                     QNSDIKVASIAKVRESEAKDSNVYDEEYYEEDTENESE"
     gene            complement(3062..4978)
                     /gene="gyrB"
                     /locus_tag="ACZPCG_07485"
     CDS             complement(3062..4978)
                     /gene="gyrB"
                     /locus_tag="ACZPCG_07485"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004841262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HIR1284945.1"
                     /translation="MAEQQYGADQIQILEGLEAVRKRPGMYIGSTSARGLHHLVYEIV
                     DNAVDEALAGYCDSIEVFINEDNSITVVDDGRGIPVDIQKKAGIPAVEVVFTILHAGG
                     KFGNGGYKVSGGLHGVGASVVNALSEWLEVQVYRDGNVYQQRYERGRTMYPLKIVGTC
                     SPDQHGTRVSFLPDKEIFEETVFDYDTLKMRLRETAFLTKNLKIVLHDEREEKHEHTF
                     HYEGGIKEFVSYLNKGKTPLYENVLYCEGTKDGVYVEVSMQHNDSYTENIYTFVNNIN
                     TPEGGTHLTGFKNALTKTFNDYARKNKLLKENEDSLSGEDIREGLTAIVSVKVEEPQF
                     EGQTKQKLGNSEARGAVDNIVSEQLTYYLEQNPTAAKAMCEKSIMAQRARAAARKARD
                     LTRRKSALEGMALPGKLADCSDKNPENCEIYIVEGDSAGGSAKTARSRATQAILPLRG
                     KILNVEKARMDKVYANAEIKAMITAFGTGIHDDFDISKLRYHKIIIMTDADVDGAHIS
                     TLLLTFIYRFMPELIKQGYVYLAQPPLYKIEKNKRVWYAYSDEELNSILMDIGRDNSN
                     KIQRYKGLGEMDAEQLWETTMDPERRILLRVTMDEETTSEVDLTFTTLMGDQVEPRRE
                     FIENNAKKVKNLDI"
     gene            complement(5012..6097)
                     /gene="recF"
                     /locus_tag="ACZPCG_07490"
     CDS             complement(5012..6097)
                     /gene="recF"
                     /locus_tag="ACZPCG_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585017.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HIR1284946.1"
                     /translation="MVIESIELKNYRNYEELHMELNQGTNILYGDNAQGKTNILESAY
                     VCCTSKSHRGAKDRDIIRFGQDESHIKLQIRKNDVPWRIDMHLKKNKTKGIAINGIPI
                     RRASELFGLANMVFFSPEDLNIIKNGPAERRRFIDMELCQLDKLYVHSLVQYNRVLLQ
                     RNRLLKELAFKPEYETTLDIWDEQLVKYGVEVITSRRLFIEQLGGIISGIHARLSGGK
                     EEISVLYDPDIREEDFKAALEKNRSQDIRQKTTLAGPHRDDINFIVNGIDIRRFGSQG
                     QQRTAALSLKLSELELVKLVSRDAPVLLLDDVLSELDGSRQTQLLSAIQDIQTMITCT
                     GLEDFVNHRFPIDKIFKVVDGKVYSQN"
     gene            complement(6117..6326)
                     /locus_tag="ACZPCG_07495"
     CDS             complement(6117..6326)
                     /locus_tag="ACZPCG_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HIR1284947.1"
                     /translation="METIKLREDYIKLGQALKAAGFVESGVDAKYAIEDGLVSVNGEI
                     AYQRGKKLVDGDVVAYQGQTIKIIK"
     gene            complement(6402..7520)
                     /gene="dnaN"
                     /locus_tag="ACZPCG_07500"
     CDS             complement(6402..7520)
                     /gene="dnaN"
                     /locus_tag="ACZPCG_07500"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HIR1284948.1"
                     /translation="MKLQFQKDAIVNGINIVMKAVPSKTTMSILECILIDASSNEIRL
                     TGNDMELGIETKVEGQILEHGKIALDAKLFSEITRRLSSQNSTITIESDQKLNTTIRC
                     ENSVFNIQGRDGEEFTYIPYIERDKYITLSQFTLKEVIQQTIFSISPNDSNKMMGGEL
                     FEVNENQLKVVSLDGHRISIRNVTLKDHYEPTKVIVPGKTLSEISKILGGDNEKEVRI
                     FFSSNHILFEFDDTIVVSRLIEGEYFRINQMLSSDYETKFSVNRREFLDCIERATILI
                     RENDKKPLILQVNDNTMELKLNSSFGSMNAELLIHKTGKDIMIGFNPKFLIDALRAIE
                     DEEINIYMMNPKSPCFIKDEEETYIYLILPVNFNAASV"
     gene            complement(7800..9170)
                     /gene="dnaA"
                     /locus_tag="ACZPCG_07505"
     CDS             complement(7800..9170)
                     /gene="dnaA"
                     /locus_tag="ACZPCG_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HIR1284949.1"
                     /translation="MIEKIKENWEKILLILKEEHEISNISYQTWLEPLSPYSFKGNTI
                     TIIVPEQTFLAYVKKKYGLLFKVTISEFLGQECEVDFKVKEQIEVQETPAAPSLIPKA
                     KAPVSPNVIQSANLNPKYTFDTFVVGSNNNLAHAAALAVAESPGEIYNPLFIYGGVGL
                     GKTHLMHAIAHFILKNNPKSKILYVSSETFTNELIDAIRNKNNITTTEFREKYRNNDV
                     LLIDDIQFIIGKESTQEEFFHTFNTLYESKKQIIISSDKPPKEIETLEERLRSRFEWG
                     LTVDIQSPDYETRMAILRKKEEMEGYNIDNEVIKYIATNIKSNIRELEGALTKIVALS
                     RLNKCDITLELAEEALKDIISPNAQREVTPELIIQVVSDHFGITPADVISQKRTKDIV
                     YPRQIIMYLCRTMTETSLQIIGRSLGKRDHTTVLHGADKITSDMQKDETLRNTIDILK
                     KKINPQ"
     gene            9291..9470
                     /locus_tag="ACZPCG_07510"
     CDS             9291..9470
                     /locus_tag="ACZPCG_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284950.1"
                     /translation="MKGIESVDREVFHRIGKKITAGCTQWGNLQKIRGFCPRMRKKVE
                     ISHTYPQVINKLWIR"
     gene            9585..10202
                     /locus_tag="ACZPCG_07515"
     CDS             9585..10202
                     /locus_tag="ACZPCG_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006778075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIR1284951.1"
                     /translation="MNKRKKDTRWLASVALMMAVVILLASTPLGMIQLPVVKATTVHI
                     PVILGAILLGPLAGSILGGVFGICSMVSNTMAPTLLSFAFSPFMSMTGISGAVKAVWI
                     SVGCRILIGFFAGWLWILLSKCKMNRMAALAVTGFMGSMVNTVAVMGSIYKLLALPYA
                     QVKNVAVDAVWGLIMATVTASGIPEAVVAAVLVAIIGKIMLKFIK"
     gene            10355..10489
                     /gene="rpmH"
                     /locus_tag="ACZPCG_07520"
     CDS             10355..10489
                     /gene="rpmH"
                     /locus_tag="ACZPCG_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HIR1284952.1"
                     /translation="MKMTFQPKKRQRAKVHGFRARMKTAGGRKVIAARRAKGRAKLTA
                     "
     gene            10594..10938
                     /gene="rnpA"
                     /locus_tag="ACZPCG_07525"
     CDS             10594..10938
                     /gene="rnpA"
                     /locus_tag="ACZPCG_07525"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HIR1284953.1"
                     /translation="MKRFPSIKKNSDFQYIYRNGKSYANRLLVMYVVRSGGEANSIGI
                     SVSKKVGNSVVRHHVTRLLREIFRLNDNKIETGLNIILVARVAAARSDYRHLEGAYMH
                     LCGLHHILKESK"
     gene            10944..11159
                     /gene="yidD"
                     /locus_tag="ACZPCG_07530"
     CDS             10944..11159
                     /gene="yidD"
                     /locus_tag="ACZPCG_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003503700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HIR1284954.1"
                     /translation="MIKKGMILLIRIYQKYLSPLKVRTHCIYTPTCSQYAIEALQKYG
                     ALKGTWLACKRILRCHPFAEGGYDPVP"
     gene            11184..12506
                     /locus_tag="ACZPCG_07535"
     CDS             11184..12506
                     /locus_tag="ACZPCG_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HIR1284955.1"
                     /translation="MNGLVGAVVLTKDSGFLGPIASILGWIMDLLFEFTSIFGIENIG
                     LCIILFTLVTKVLMIPLTIKQQKSSKLMTVMQPEIAAIQKKYKGKENDQKAAMMMQAE
                     IKAVYEKYGTSMTGGCLPLLIQMPIIFALYRVIYNIPAYVQSVRGYYETVISHLPSGY
                     QTQQAFTQLAEAHKMVGEKFDYNNVNTVIDLLYTFTAQQWDSFVAAFPAVGQAVTDAG
                     VNAVEAIENMQSFFGLNIAYTPFQVIVNFFEQQGGVTLGMAAAALAIPILAGLSQWYS
                     TKLMTANQSQPADDDNPAASMMKSMNITMPLMSVFFCFSFASGIGLYWVAQSVFTIIQ
                     QVWINSYLNKVDMDDLVQKNIEKTNKKRAKKGLSPVKVGNVDEMLKRMEEKESREESA
                     RMEKIAKTKVQVEESNKYYNQDAKPGSLAAKANMVAKYNERQEKGKKK"
     gene            12518..>12636
                     /locus_tag="ACZPCG_07540"
     CDS             12518..>12636
                     /locus_tag="ACZPCG_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HIR1284956.1"
                     /translation="MDMITVSAKTVDEAVTKALIELKTTSDKLEYEVVEEGSKG"
BASE COUNT         3310 a         2841 c         2770 g         3715 t
ORIGIN      
        1 attttcaggg ttttacatac tgtttaatct tcaattttca aggatctttg ctgttgtttc
       61 ctcagcagca acttttatat cttatcatag tcgctcgcgt ttgtcaacaa tttttttatt
      121 tttatttttt cgtttttgcc gattgtctca gcgagtatca atgatatcac acttaagcgc
      181 tcatgtcaac cactttttta acaattttca aaaacttttt taggaaaatg gagcggagaa
      241 agagggattt gaaccctcgc gccggtttcc cgacctacac ccttagcagg ggcgcctctt
      301 cggcctcttg agtatttctc cgaattcaaa tttccatata attgctcctg acaggtatat
      361 ctgcccgcca gacgcatagg gtattatact aaagggttct tattatgtca agagctattt
      421 ttcaattttt ttacataatt ttttataaac gaaaaaccta aacgaaaaac cggcccagtc
      481 gaaactctga ctgaaccggt ttttcttatc ggaatttatt cggactcatt ttccgtatct
      541 tcttcataat actcttcatc ataaacattg ctgtccttgg cttcgctttc gcgtaccttg
      601 gcaatgcttg ctaccttaat atctgaattt tgatcaatag acataagttt tacaccagaa
      661 gtatttcttc cgattactga aatatcatct acagacatac ggataataat gccttctgtg
      721 gtaatcagca tgatttccct tccgtcattt accagcttgg cgcctaccag gttccctgtc
      781 ttttccgtta tcttatagca gagcacaccc tttccgcctc tgaactgagg cttaaactcc
      841 tcaatcggcg tacgcttgcc cataccgttt tcagatacta caagaagctc ctctccctgg
      901 ctttccatct gcatgccaat aacctcgtca tcctcatcaa agcgcatacc gatcacgccc
      961 atggatacac ggccagtggt tcttacatcc ttttccttaa agcggatgca cattcccttc
     1021 ttggttacca ggaaaatatc ctgcttatcg tctgtagcct ttacttcaat gagttcgtct
     1081 ccctctttca gtacaatagc ctgaagtccg tttttgcgta cattgccgta ctctaccata
     1141 ggggttttct ttaccatacc ctctttggtt gccataaaga gatactgatc ctcattaaac
     1201 tccctcatgg gaataatggc agtaatcttt tctcccggct gaagctgcag aaggtttaca
     1261 atagcggttc ctcgagcagt gcggcctgcc tccggaatct catatgcctt taagcggtat
     1321 acgcggcctg tatttgtaaa gaacatcatg tgatggtggt tggttgtcat aagaaggtct
     1381 tcaatatagt cctcctcaat ggtctgcatt cccttaatac cttttccgcc ccggttctgg
     1441 ctcttgaaat tatctgcatc catgcgttta atatagccta aatgagtcat ggctaccact
     1501 gtatcctcat ccgggatcag atcttccata gacatatcat catcaaagcc aatagcagta
     1561 cggcggtcat ccccgtattt ggccgcaatg atattgattt cctctctgat taccataaga
     1621 agcttcttct catctgccag aattgccttt aactgggcaa ttttagcctg cagctcctta
     1681 aattcatttt ccagcttttc ccgttccaga ccggtaagag cacgcagtct catatctaca
     1741 attgcctgag actgggcatc cgaaagcccg aaccgctcca tcaactgtgt cttggcctct
     1801 gccacgttgg cagaaccgcg gataatacgg atgacttcat caatatgatc caatgcgatc
     1861 agcagaccct gcaaaatatg agcccgctcc tctgccttat tcaaatcata ctgagttctt
     1921 cttcttacta cctcttcctg atgggaaata taaacctcca gcatctgatg aaggttcagg
     1981 atctttggct gattattcac taatgccagc ataatgacac caaaggtatc ctgaagctgg
     2041 gtatgcttaa gaagctggtt taaaattacg ttcgcattca catcacggcg cagttcgata
     2101 ttgatgcgca taccttcacg gttggactca tctccaatat aagtgattcc gtcaatcttc
     2161 ttttctttca caagatccgc aatcttctca atgagacggg ccttatttac catataaggc
     2221 agctcggtga caataatccg ggatttaccg tttgccatag tctcgatatt ggtaacagct
     2281 ctcaccttga tcttggcacg gcctgtacgg taagcctcct caatgcctct gcggcctaaa
     2341 atagtagctc ctgtaggaaa atccggcccc ttgatgactt ccataagctc ctctatggag
     2401 gtatcacgat cctcattgac ccgattgtca atcatcaggg aaacagcccc tatgacctct
     2461 ctgagattgt gaggcggaat gttagttgcc atacctacgg cgataccgga ggtaccgttt
     2521 accagaagat ttgggaaacg tgccggaaga accattggct ccttctccgt ctcgtcaaag
     2581 ttgggagcaa agtctacggt gtccttattg atatcagcca gcatttccat ggaaatacgg
     2641 cttaaacggg cctctgtata tcgcatggct gctgctccgt ctccgtccac ggaaccaaaa
     2701 tttccatggc cgtctaccag cggataacgg gtggaccagt cctgggccat atttaccaga
     2761 gctccgtaga tggagctgtc gccgtgagga tggtatttac ccatggtatc acctacgata
     2821 cgggcacatt ttctgtgtgg cttatccggt ccgttgttta actctatcat ggaaaaaagc
     2881 acacggcgct gaacgggttt taaaccgtct ctggcgtccg gaagagcccg ggcgctgata
     2941 acgctcatgg catagtcaat ataggactgt tccatggtct ttttcaggtc tacgtcatgg
     3001 accttgtcaa atacatttgg ttccatagta tatctccttt atgttcgatc tcatttgcag
     3061 cttaaatatc cagatttttc acctttttgg cattattttc aataaattcc cttctcggct
     3121 ctacctggtc gcccatgaga gtggtaaagg tcagatccac ttctgaggtg gtttcctcat
     3181 ccatggttac acgcagaagg atacggcgtt ccggatccat ggttgtctcc cagagctgct
     3241 ctgcatccat ctctccaagt cccttgtagc gctggatctt attgctgttg tcgcggccaa
     3301 tgtccataag aatggaattc agctcctcat cgctgtacgc ataccataca cgcttatttt
     3361 tttcgatttt atacagaggc ggctgtgcca gatatacata tccctgcttg atcagctcag
     3421 gcataaaccg gtaaataaaa gttaaaagga gggtgctgat atgggcgccg tccacatccg
     3481 catcagtcat gataatgatc ttgtggtaac gcagcttgct gatatcaaag tcatcgtgaa
     3541 tacccgtacc aaaggctgtg atcatggcct taatctctgc attggcataa accttatcca
     3601 tcctggcctt ttcaacattt aagatttttc ctcgcagggg cagaatggcc tgggtggctc
     3661 tggaacgggc cgtctttgca gaaccgcctg cggaatctcc ctctacgata taaatttcac
     3721 aattttcagg attcttatcg gaacaatctg ccagctttcc cggaagagcc atcccctcca
     3781 gggcagattt gcggcgggtt aaatctctgg cctttctggc tgccgcacgg gctctctgag
     3841 ccataatgga cttctcgcac atggcttttg ctgctgtggg gttctgttct aaataataag
     3901 tcagctgctc gctcacaata ttgtcaaccg cacctctggc ctcactgttt cccagcttct
     3961 gcttggtctg tccctcaaac tgaggctcct ctacttttac gcttacgatc gccgtaagcc
     4021 cctctcggat atcctcaccg gaaagactgt cctcattttc ctttaaaagc ttattctttc
     4081 ttgcataatc attaaaggtc tttgtaaggg catttttaaa tcctgtcaga tgagtaccgc
     4141 cctcaggagt attgatgtta tttacaaagg tgtaaatatt ttctgtatag gagtcattgt
     4201 gctgcatgga cacttccaca tatacgccgt cctttgtacc ttcgcagtaa aggacatttt
     4261 catacagcgg cgttttgcct ttattcagat aggatacaaa ctccttgata ccgccctcat
     4321 agtggaaggt atgctcatgc ttctcctccc tctcatcatg gagaacgatt ttcagatttt
     4381 ttgtaaggaa tgcagtctcc cgcaggcgca tttttaatgt gtcatagtca aatacggttt
     4441 cctcaaaaat ctccttatca ggaaggaagg aaacacgggt tccatgctga tcaggactgc
     4501 atgttcccac aatctttaag ggatacatgg ttctgcccct ttcgtaacgc tgctggtata
     4561 cgtttccatc tctgtatacc tgtacctcca gccattcaga aagcgcattt acaacggatg
     4621 cacccactcc gtgaaggccg ccggatacct tataaccgcc gtttccaaat tttccccctg
     4681 catgaaggat ggtaaataca acctctaccg caggaatccc tgcttttttc tgtatatcta
     4741 cgggaatacc gcggccgtca tcaaccacgg taatggagtt gtcctcatta ataaaaacct
     4801 cgatggaatc acaatatccc gccaatgcct catctaccgc attgtctaca atctcataaa
     4861 ccagatggtg aaggcccctg gcagaggtgc tgccgatata cataccggga cgctttctta
     4921 ctgcctcaag tccttccaga atctggatct gatctgctcc gtattgctgc tctgccatgt
     4981 tctatatctt cctttctttt caggtgttcg tctaattctg gctgtatacc tttccgtcca
     5041 ctactttgaa gattttgtca atggggaacc tgtgattaac aaaatcttcc agccctgtac
     5101 aggtgatcat agtctgaata tcctgtattg cggacagaag ctgggtctgc cggctgccat
     5161 caagctctga gagaacgtca tccagcaaaa gaaccggtgc atccctgctc acaagcttta
     5221 ccagctcaag ctctgaaagc tttaaagaca gggctgccgt acgctgctgg ccctgagagc
     5281 cgaaccggcg gatatcgatt ccatttacaa taaaattaat atcatccctg tggggacctg
     5341 caagcgtggt cttctgacgg atatcctgag aacggttctt ttccagtgcg gctttaaaat
     5401 cctcctcccg gatatccgga tcatagagaa ccgatatttc ttcctttccc cctgataaac
     5461 gggcgtggat accgcttatg atccctccca gctgctcaat aaacaaacgg cgggaagtga
     5521 tcacctctac cccgtatttt accagctgtt cgtcccaaat gtccagcgtt gtttcatatt
     5581 ccggtttaaa ggccagttct tttaagaggc ggtttctctg cagcagcacc cggttatact
     5641 gaaccaggga gtggacatac agcttatcca gctggcaaag ctccatatca ataaaccgcc
     5701 tccgttctgc cggaccgttt ttgataatat ttaagtcctc cggggaaaag aataccatat
     5761 tcgccaatcc aaacagctcg ctggcccgcc ggataggaat cccgttgatg gctatgccct
     5821 ttgttttatt tttctttaaa tgcatatcaa tgcgccaggg aacgtcattt ttccggatct
     5881 gcagctttat atgggattca tcctgtccaa aacggatgat atccctgtcc tttgcccccc
     5941 ggtgggactt ggaagtgcag caaacatagg cagattccag aatatttgtc tttccctggg
     6001 cattgtcacc atagagaatg ttggttccct gattcagttc catatgcagt tcttcataat
     6061 tacggtaatt cttaagctct atggactcaa tgaccatgga tgttctctct tcatgattat
     6121 tttatgatct ttatagtctg gccctgatag gcaaccacat ctccgtctac cagtttcttt
     6181 cccctctgat aagcaatttc accgtttaca gataccaagc catcctcaat cgcatatttg
     6241 gcatctacac cggattctac aaagcccgct gcctttaagg cctgtcccag cttgatataa
     6301 tcttctctta atttaattgt ttccattgtt ttcctttcca agcacaggag caccagagaa
     6361 atgtctttca ccggcgcaca tgctgatgta tatttatgca tttaaacaga agctgcgtta
     6421 aaatttacag gaagaatcag ataaatatat gtctcttcct catccttgat aaagcagggg
     6481 gacttgggat tcatcatata aatattgatt tcctcatcct caatggcgcg gagagcgtca
     6541 ataaggaact tgggattaaa gccgatcata atatcctttc cagtcttgtg gatcaaaagc
     6601 tccgcattca tggagccaaa gctggaattt aactttaatt ccatggtatt gtcatttacc
     6661 tgaaggatca ggggcttttt atcattttca cggatcagga tcgttgcccg ctcaatacaa
     6721 tccaaaaatt ctctccggtt tacagaaaat ttcgtctcgt aatcactgga aagcatctga
     6781 ttaatacgga aatactcgcc ttcaatgagg cgggaaacca cgatcgtatc gtcgaattca
     6841 aataaaatat ggttggagct gaagaaaatc cgaacctctt tttcattgtc tccccctagg
     6901 atcttgctga tctcgcttaa ggtttttcca ggaacgatga ccttagtggg ttcatagtga
     6961 tcctttaagg taacatttcg gatggaaata cggtggccgt ccagtgaaac cactttcagt
     7021 tggttttcat ttacctcaaa aagctctcct cccatcattt tattactgtc attgggagaa
     7081 atggagaaaa tagtctgctg gatcacttcc tttaaggtga attgagaaag ggtgatatat
     7141 ttatcacgtt caatataggg aatataggta aattcctccc cgtcccgtcc ctgaatatta
     7201 aaaacagaat tttcacatcg gatagtggta ttcagctttt gatcggattc aatggtaatg
     7261 gttgaattct ggctggataa acgacgggtg atttcagaaa aaagcttggc atccaaagca
     7321 attttaccgt gttccaggat ctggccttct acctttgttt cgatacctag ttccatatca
     7381 tttcctgtca gacggatttc attggaagaa gcgtcgatca ggatacactc taaaatagac
     7441 atggttgtct tggatggaac ggctttcata acgatgttga tgccatttac aatggcgtcc
     7501 ttctggaatt gcagcttcat gattgttgat aacttccttt ctgaatgaag taaaaaaggc
     7561 ggaacctaaa tagataaaga tatagttaat ttccgccgta atcgtagtag gggctgtggg
     7621 tttgtgtaaa agtgtgtaaa tcccgtaaaa atggggataa atcactgtgg aagagactgt
     7681 ggaaagaatc cacaaaacct gggagtaagt taagatttat ccacaggcgg ttaaaaatga
     7741 atttgagcct caaaaatacc caaaggttat caaccattta tccacagtga ttccacgtct
     7801 tattgtggat tgatcttctt ctttaatata tcgatcgtat ttcgtaaggt ctcatccttc
     7861 tgcatgtcgc tggtaatctt atcagcaccg tggagaacag tggtatgatc cctttttcca
     7921 aggctgcggc cgatgatctg taaggaggtt tccgtcatgg ttcggcagag gtacatgatg
     7981 atctgtcttg gatatacgat atccttggtc cgcttctggg aaataacatc agcgggagtg
     8041 atgccgaaat gatcggatac tacctgaatg atcagctccg gagtcacttc tctctgggca
     8101 ttgggagaaa tgatatcctt taatgcttcc tctgccagtt ccagagtaat atcacattta
     8161 tttaagcggg aaagcgctac gatttttgta agagcgcctt ccagttcacg gatattggat
     8221 ttgatattag ttgcaatgta tttgattact tcattatcaa tattgtagcc ttccatctcc
     8281 tctttttttc ggagaatggc cattcgggtt tcgtagtccg gggactggat gtccacggtc
     8341 aggccccatt caaaacggga acgcagccgt tcctccagag tttcgatctc cttgggcggt
     8401 ttatcagagg agatgatgat ctgttttttc gattcgtaca gggtgttgaa ggtatggaaa
     8461 aattcctcct gggtactctc ctttcctata ataaactgga tatcatcgat caggagaacg
     8521 tcgttgttgc ggtatttttc ccgaaactct gtggtggtaa tattattttt gtttcggatc
     8581 gcgtcgatca gctcattggt aaaggtctcg gaactgacgt aaagaatctt ggatttcggg
     8641 ttgtttttca gtataaaatg agcgattgca tgcatcaaat gggtttttcc aagtcctacg
     8701 cctccgtaga taaacagcgg attgtagatt tcgcccggag attccgctac cgcaagggct
     8761 gccgcgtggg ccagattgtt gttggagccg actacaaagg tatcaaaggt atactttggg
     8821 tttaaattgg cgctctggat cacattcggg cttacgggag ctttggcttt tgggataagg
     8881 gagggggctg caggcgtttc ctgaacctct atctgctcct ttaccttaaa atccacctca
     8941 cattcctgac caagaaattc agagatggta actttaaaaa ggaggccgta ttttttcttt
     9001 acataagcta aaaatgtctg ttctggaaca ataatcgtaa tggtatttcc cttaaaagaa
     9061 taggggctta aaggttccag ccatgtctgg tatgaaatgt ttgaaatttc atgctcttcc
     9121 tttaaaataa gcagaatttt ttcccagttt tcttttattt tctctatcat tttctgtctc
     9181 ctgaaactgt attgcagaat tgcccaatct aaatacattg tatactaacg gtgtgaattt
     9241 ttcaatcaaa agtgtaaata tctgtggata actttgtagg aaacctgtgg atgaagggaa
     9301 tcgaaagcgt ggacagagag gttttccaca gaatagggaa aaaaataact gcaggttgta
     9361 cacaatgggg aaacttacag aaaataaggg gattttgtcc acgaatgagg aaaaaggtcg
     9421 aaatatccca tacttatcca caagttatca acaaattgtg gataaggtga aaaaaggaac
     9481 cgtggattta gaggaaaaat attgacgggt gaaaaaaacg ccgctataat gcctgtgtaa
     9541 tgcgttgtat cttaacatta agaacagcag caaggaggaa aaaaatgaat aagagaaaaa
     9601 aagacacccg ttggcttgca agtgttgcac ttatgatggc ggtggtgatc cttctggcaa
     9661 gtacgccgtt aggaatgatc cagctgccgg tggtgaaggc gacaactgtg catatccctg
     9721 tgattttggg agccattctt ctggggcccc tggcaggttc catcctggga ggagtgttcg
     9781 gtatctgttc tatggtgagc aacacgatgg cgcctaccct gttatccttt gcgttttcac
     9841 cgtttatgag catgaccggg atttcaggag ccgtaaaggc agtgtggatt tctgttggat
     9901 gccggatttt aatcggtttt tttgcaggat ggctctggat tttactgtca aaatgcaaga
     9961 tgaaccgcat ggcggcgctg gctgtaaccg gttttatggg atctatggta aatacggtgg
    10021 ctgtgatggg aagtatttat aagcttttgg cgcttccgta tgcccaggta aagaatgtgg
    10081 ctgttgacgc agtgtgggga ctgatcatgg ctactgttac cgcatcaggg attccggagg
    10141 cggtcgtagc tgcagttttg gtggctataa ttggaaagat tatgctgaaa ttcataaagt
    10201 aagcggtttc agggcgtaaa agcctgtatt ttaataaaaa attgcttgac ggaggcggat
    10261 ttcttctata taattgagaa gatgtttaat tatactagtc aaatgtgtgt tatatcacat
    10321 taccagataa tatttgatag gaggtgcccg gattatgaag atgacatttc agcctaagaa
    10381 aagacagaga gcaaaagttc atggcttcag agcaagaatg aagacagcag gcggtagaaa
    10441 agttattgct gccagaagag caaaaggtag agcaaagtta accgcttaag aattgcaagg
    10501 ccacattttc atgtggcctt tttcttacgt ctacaataaa caagtcggaa cccagtacat
    10561 aaacctgttc cggcaaattt ccaggaggat cgaatgaagc gatttccttc cattaaaaaa
    10621 aacagtgact ttcagtatat atatagaaat ggaaagtcct atgccaaccg tcttttagtc
    10681 atgtatgtgg taagaagcgg gggagaagcg aacagcatcg ggatatcggt gagtaaaaaa
    10741 gtaggcaaca gcgtggtacg ccatcatgtg accaggctgc tgcgggagat attccggttg
    10801 aatgataata aaattgagac tgggttaaac atcattctgg tagcccgtgt ggctgccgca
    10861 agatctgatt acagacattt ggagggtgcc tatatgcacc tgtgcggact gcatcacatt
    10921 ttaaaggaat cgaagtgaga gttatgataa aaaaaggaat gatcctgctt atcaggatct
    10981 accagaaata tctgtcgccc ttaaaggtaa ggactcactg tatttacacg ccaacgtgct
    11041 ctcaatacgc cattgaggca cttcaaaagt atggcgcgtt aaaaggtaca tggttagcct
    11101 gcaagaggat ccttcgttgc catccctttg cagaaggcgg ttatgatcca gttccgtaga
    11161 aagttccata aggaggtagc gttttgaacg gtttagtcgg cgcagtagta ttgacaaagg
    11221 acagcggttt tctggggccg atcgcaagta tccttggctg gatcatggac ctgctttttg
    11281 agttcaccag catattcggc attgaaaata tcggcctttg cattatcctg tttacactgg
    11341 ttacaaaggt tcttatgatt cccttgacca taaagcagca gaaatcatcc aagctgatga
    11401 cggttatgca gccggaaatt gcagcaattc agaagaaata caaaggaaaa gaaaatgatc
    11461 agaaagccgc gatgatgatg caggctgaga tcaaggcagt atatgagaaa tacggcacat
    11521 ccatgacggg cggctgtctt cctcttctga tccagatgcc gatcattttt gcactgtaca
    11581 gagttattta caatattccg gcttatgtac agtctgtcag aggctattat gagacggtaa
    11641 tttcccatct tcccagcgga taccagaccc agcaggcgtt tacccagctg gcagaggccc
    11701 ataagatggt gggtgagaaa tttgattata acaatgtaaa cacggtaatt gaccttttat
    11761 atacctttac agcccagcag tgggacagct ttgttgccgc attcccggca gtgggacagg
    11821 cagtgacaga tgcaggcgta aatgcagtgg aagccattga aaatatgcag agcttctttg
    11881 gcctgaatat tgcgtatact ccatttcagg tgatcgtgaa tttctttgag cagcagggcg
    11941 gcgtaactct gggtatggca gctgcagctc tggcaattcc gatccttgca ggcttaagcc
    12001 agtggtacag tacaaagctt atgactgcca atcagtctca gccggctgat gacgataatc
    12061 cggctgccag catgatgaaa tccatgaata tcaccatgcc tctgatgtcc gtattcttct
    12121 gcttctcctt tgcttccggt atcggtcttt actgggtggc tcagagtgtg ttcactatca
    12181 tccagcaggt atggatcaat tcctacctga acaaggtaga tatggatgac ctggtacaga
    12241 agaatattga aaagaccaat aaaaagcgtg ccaagaaggg cctttctcct gtcaaagtcg
    12301 gaaatgtaga tgagatgtta aagaggatgg aagagaagga atccagagaa gagtctgctc
    12361 gtatggaaaa aattgccaag acaaaggttc aggtagagga atccaataag tactataatc
    12421 aggatgccaa accgggaagt ctggcagcta aggcgaatat ggtagctaaa tacaatgagc
    12481 gtcaggagaa gggaaagaaa aagtaggagg gtttcgtatg gatatgatta cggtttctgc
    12541 aaaaacagtg gatgaagcgg ttacgaaagc tctgatcgaa cttaagacaa ccagcgataa
    12601 actggagtat gaggttgtgg aggaaggaag caaagg
//
LOCUS       DBMDUH010000073        12561 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_2601_length_12561_cov_7.217897, whole genome shotgun sequence.
ACCESSION   DBMDUH010000073 DBMDUH010000000
VERSION     DBMDUH010000073.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 12561)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12561)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12561
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2601_length_12561_cov_7.217897"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1838)
                     /locus_tag="ACZPCG_07545"
     CDS             complement(<1..1838)
                     /locus_tag="ACZPCG_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284957.1"
                     /translation="MCGIRRREMKMVLVLLWAAGAIVLGIGVFELFLTGSLGEILKKE
                     ETKTMLAQVLAVFLWNLLWLSRVKNRKAQILGISVGSLLFAWCHQMLVPAVISGVWIA
                     VLICLGRWLNGWLMGGRKRMQDLTRKEWENIHWAETLALGLVTGSALWMVIVCVISLT
                     GHGGVPFWRILALILTAGTLAVCAAIQIRRKNESRKQQERKAVPNREAFWQAVLPDTF
                     PQALLWAFILTMVLLQAGRLNIELDYDSLHYGLRSGFVLDNGKGIYENLGMNNLVYSY
                     SKGLEVLNLPLSGTPTYGFVLAFSFWTTVGTVLLAGHIGGRNGGKTMALWTAAVCAAI
                     PGIMNMAVSAKSDSITLLYQLIIYDFLCLAIGKKEQGKQAVPWLLFAVAAYIVTLVFK
                     PTALVFSTALGAAGLLCLLLGKNLKIGNKRGVFLWMLPLMAAAGLWYRTWRITGVPIT
                     SIFAGMCEKIGFEVKYPFNFSRVIGDPWALSAGEKAERLMERLKGILLAPVGEDMDHV
                     IIAWGTGLVTILLILWCINLIFRLRSGKQSGLFMNSHDRFDGVLTGILLAGSVLSIYT
                     LSQVDGNYFMLVYAVLGISAVRMTGTWIKARKEAGNRWAHKSLCFLA"
     gene            complement(1854..2639)
                     /locus_tag="ACZPCG_07550"
     CDS             complement(1854..2639)
                     /locus_tag="ACZPCG_07550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1284958.1"
                     /translation="MRYKHVFAIPAYGQSPWLETCIRSLKAQSQPSPVILCTSSPSEF
                     LEQMAEKYELPLYVRRGESSIRDDWNYAYEMADGEYVTIAHQDDVYHKEYAAELLKAA
                     GKWPDMTVFTTDYVITKGGKTITGDRLLWVKRILRLPLRCPAFNGFTWVKRLPLMLGN
                     SICCPATTYHKSLLGEPLVRSEYSYALDWDNLVQLAERPGRFICREKPLMYYRVHDGA
                     ATKACMEDNRRAKEEREMFARFWPEPVVRFLMRGYASAYKEYE"
     gene            complement(2629..3279)
                     /locus_tag="ACZPCG_07555"
     CDS             complement(2629..3279)
                     /locus_tag="ACZPCG_07555"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006771681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose-7-phosphate isomerase"
                     /protein_id="HIR1284959.1"
                     /translation="MNGSKVDLILKELMERYPVLMPVKEDIFKTYEILRDAFLQDGTL
                     LIAGNGGSCADSEHIVGELMKGFVKRRPVTSAFEQKLKEAEPELGEELAKRLQGALPA
                     IALTGHPALSTAFLNDVDGSMGFAQQVYGYGRKGDVFLGITTSGNSKNILYAAAVAKA
                     AGLKVIGLTGRDGGRLKAMTDACIVVPEQETFKIQEFHLPVYHALCLMLEEYFYEV"
     gene            complement(3323..3898)
                     /gene="gmhB"
                     /locus_tag="ACZPCG_07560"
     CDS             complement(3323..3898)
                     /gene="gmhB"
                     /locus_tag="ACZPCG_07560"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HIR1284960.1"
                     /translation="MEKIVFLDRDGTLNEEVNYLHHPEDMHLLAGVPEALRRLKEAGF
                     KLVVVTNQAGVARGYYTEEDVKVLHRYMNELLAAEGAEIDGFYYCPHHPVHGIGSYKK
                     ECSCRKPGIGMFLQAEERFTADKSHSYMIGDKLLDTQAGKNYGVTSILVGTGYGKELY
                     EASRAKKDEAPSYDYYAENLPQAADWILRRQ"
     gene            complement(3912..4982)
                     /locus_tag="ACZPCG_07565"
     CDS             complement(3912..4982)
                     /locus_tag="ACZPCG_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003524189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP family kinase ATP-binding protein"
                     /protein_id="HIR1284961.1"
                     /translation="MVIRGRAPLRVSFGGGGTDVAPFCEEQGGAIIGSTINKYAYCSI
                     VPREDDQIIVHSLDFDMTVKYNTKENYVYDGRLDLVTAALKAMEISQGCEVYLQCDAP
                     PGSGLGTSSTVMVAMLTAMARWKGIMMDAYAMADLAYQVERLDLKIDGGYQDQYAAAF
                     GGFNFIEFHGRNNVVVNPLRIKKDIIHELQYNLLLCYTGNIHVSANIIRDQVQNYEKK
                     DAFEAMCEVKALAYAMKDELLKGNLHSFGKLLDYGWKSKKRMSSKITNPQIDMLYDEA
                     IKAGALGGKLLGAGGGGYLLMYCPYNVRHKVAARMEQAGGQLADWNFELRGAQSWVAD
                     EERWKYDQVKVHMPNKIYTFDL"
     gene            complement(5006..5713)
                     /locus_tag="ACZPCG_07570"
     CDS             complement(5006..5713)
                     /locus_tag="ACZPCG_07570"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007717337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR1284962.1"
                     /translation="MQAILLAGGLGTRLRSVVSDRPKPMALIQEKPFMEYVTRELVRN
                     GVRDIIFAVGYKGSMVEEYFKDGSDFGFHAAYAYEETLLGTAGAIKNAGKFITEDRFF
                     VLNADTFYQIDYTRLVRQQDEQQLDMALVLRRVPDVSRYGQAVLDEKGILTGFNEKTQ
                     EAKSGTINGGIYLMKKSLLDEIPEGKVSLENDRIPCWLSEGKRLGGFVNDGYFIDIGI
                     PEAYFQFQRDVETGVVR"
     gene            complement(5837..9859)
                     /locus_tag="ACZPCG_07575"
     CDS             complement(5837..9859)
                     /locus_tag="ACZPCG_07575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007717339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR1284963.1"
                     /translation="MNDISYEILDLFKKYGSDPEGIQKILENDSRPEVLYALSDVRKN
                     LFDWMELKKCENVLQLDSGYGVLTGLFAERCAHVTVTDRRDENLVVNEQRNSSADNIS
                     YIKEIDWKKEDHRAAYDLVVLESSRPDMDLKAVVKEASQYVKQGGRLVFSCENALGLC
                     FLSGAVHDEDESAFTKGELEAAFREAGLETVEFYYPMPEYKRAQTVYSDRYLPGKGDI
                     PHVTAVYDRQRWACIHEDEISDKLVQEKAFGLFANAYLAVASKEQEPFDTIFAKYNRT
                     RKPQFQIRTRILEENGKRYVEKAALTEASGAHIGSMEEKHEKLSKVNPEVRVLEPHFS
                     EDKQAVRFEFLTGKTLAEELGGQIRGKKAPVEAIQAAMEQVFSKAALRPESFYVTPEF
                     LEVFGRNPAEDSQDSASGELEQQLSALSDASYAVSNIDGLFENLMVSGGKLYCLDYEW
                     VFDFPVPAGFVRYRNLVYFYYKYEGLMDYENAADFLKEFGIGEELSGLYAAMEESFQS
                     WVHGDGTQGYMGNYKQRLVTLEELKAQEKELDQARERINQLQEEVEERNIQVKKDQEI
                     LRLTNNHVKNLEIMIKDLRHEIDELGKLATYLNGHEAAVYKLRRKLGVQVNKAFPKGT
                     RKRKVLNYCFHTVRHPARYMKMYATKEGRNLIEGDFKIGEGYLTGGHLSFPQYPGEGK
                     EGKNGWEGPLVSIVIPCYNQIHYTYACLQSILEYTKDVTYEVIIADDVSTDATAEISR
                     FVDGLVICRNQTNQGFLRNCNQAAKAARGKYIMFLNNDTKVTEGWLSSLVKLIESDPS
                     IGMVGSKLVYPDGRLQEAGGILWSDGSGWNYGRLDDPDKAEYNYVKDVDYISGAAILL
                     SVQLWKQIGGFDERFAPAYCEDSDLAFEVRKAGYRVVYQPLSKVIHFEGVSNGTDVNG
                     TGLKRYQVENSKKLREKWADEFKRQSVNDGNPNPFRARERSQGKKVILVVDHYVPTFD
                     KDAGSKTTYQYLKMFLKKGYVVKFLGDNFLHEEPYSTALQQMGIEILYGDDWASGIWD
                     WLKRNKDEIDFAYLNRPHIATKYVDFIKENTNIKVIYYGHDLHFLRLGREYALTGDIS
                     IKREADYWKAIELSMMRKAAISYYPSYVEVDAIHKIDPEIPVKAITAYVYDEFLANIQ
                     EDFAKREGLLFVGGFAHPPNADAVLWFAQEIFPLIREKLPEINFYVVGSKVTDEIKAL
                     EQPGNGIIIKGFVSEEELAELYASCRIVVVPLRYGAGVKGKVVEAIYNGAPIVTTGTG
                     AEGIPEVETILEIEDDPAAFAEKCVSLYNDPARCRSMCHATQDYIRRHFSLEGAWKVI
                     EEDFRDSSEQCSHAEFHVEK"
     gene            complement(9852..11276)
                     /locus_tag="ACZPCG_07580"
     CDS             complement(9852..11276)
                     /locus_tag="ACZPCG_07580"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1284964.1"
                     /translation="MEVREQENAISVRDVTKVYRLYDKPIDRLKESMSISHKNYHRDF
                     YALSGLSFQVKKGETVGIIGTNGSGKSTILKIITGVLTPTTGQVDVNGKISALLELGA
                     GFNMDYTGIENIYMNGTMMGYTKKEMDAKLQDILDFAEIGDFVYQPVKTYSSGMFVRL
                     AFALAINVDPEILIVDEALSVGDVFFQSKCYRRMEEIRKKGTTILMVTHDMGSIIKYC
                     DRVVLLHKGEFIAEGPAGKVVDMYKKILAGKLEELKAELAAGVHKTDQPSGTEDDLSD
                     FSGGMDVDGLVDAQLAKKTFLDNHESAGTTNQHPKGLMRDQMTINSDRTEYGDGRAEI
                     YDLGLLDARGNITNLLLKGEMFTIRECIRFHADIQSPIFTYTIKDKKGTELTGTNTMF
                     EGADIQPVKKGEEYVVEFTQKMTLQGGEYLLSMSCTGFEAGEHTVYHRLYDVTNITVI
                     SNKNTVGVYDMESQVKAEKRKADE"
     gene            complement(11377..12258)
                     /locus_tag="ACZPCG_07585"
     CDS             complement(11377..12258)
                     /locus_tag="ACZPCG_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1284965.1"
                     /translation="MTLRHLRIFVTVCETGSMTAAAQILYIAQPAISLAVSEMEEHYK
                     VRLFDRISRRLYLTDSGRQALEYARHIIALFDEMEQGVGGGQAAKELRIGTSITIGSC
                     LLPGYIRELKERFPSLTVQAAVGNSEAIEQKLLENQIDIGIIEGMVHSPYIHCRTFPG
                     DRLVFICPPAHPFAGHTLKSAEELAGQDLILRERGSAGREVLDGILAAGEISVKPLWE
                     SVSNEAVLEGVKAGLGLSLLPYYLVRDSLEKGQVKEFFIRGLALSRKFSVIYHKNKFL
                     TKEARTLIGLAAGEEEG"
     gene            12428..>12561
                     /locus_tag="ACZPCG_07590"
     CDS             12428..>12561
                     /locus_tag="ACZPCG_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HIR1284966.1"
                     /translation="MEAVSHHEKRSVILKKLFISTFYLSAFTFGGGYVIVTLMKKTFV
                     "
BASE COUNT         3047 a         3575 c         2626 g         3313 t
ORIGIN      
        1 gccagaaaac agaggctttt gtgagcccat cgattccctg cctctttccg ggcttttatc
       61 cacgttcctg tcatgcgcac tgcgctgatt ccaagaaccg catataccag cataaaataa
      121 tttccgtcca cctggctcaa tgtataaatg cttaaaacag agcctgccag aaggattcct
      181 gtaagaacgc cgtcaaaccg gtcatgactg ttcataaaaa gcccggactg ctttcctgag
      241 cgaagccgga aaataaggtt tatgcaccac aaaatgagaa ggatggtcac aagccccgtt
      301 ccccaggcaa tgatcacatg gtccatatct tctcccacag gtgcaagaag aatccctttc
      361 agacgctcca tgagcctttc tgccttctca cctgccgaca gagcccaggg atccccgatg
      421 acccgtgaaa aattaaaggg atattttacc tcaaaaccga tcttttcgca cattccggca
      481 aaaatagaag tgatcggtac tcccgtgatc ctccatgtgc ggtaccacaa tccggctgca
      541 gccataagcg gaagcatcca gaggaaaact ccccttttat ttccaatttt taaatttttc
      601 ccaagcagca ggcataacag tcctgccgcg cccagtgccg tagaaaacac cagtgcggta
      661 ggcttgaaaa caagggtcac aatataagcc gccactgcaa agagcagcca tggaaccgcc
      721 tgtttcccct gctccttctt accaatggca aggcagagga aatcataaat gatcagctgg
      781 tacaaaaggg taatgctgtc gctctttgca gacactgcca tattcataat gcccggaatg
      841 gcagcacata cagcggctgt ccacaaagcc atggtttttc cgccgtttct cccgcctata
      901 tgtccggcca gaagcactgt tcccacagtt gtccagaagg aaaaggccag gacgaaacca
      961 taggtaggcg tacctgacag aggaaggttt aaaacctcca gccccttgct gtagctgtac
     1021 accagattat tcattcccag gttttcataa attcccttcc cattatccag cacaaaaccg
     1081 gaacgcaggc cgtagtgaag ggaatcataa tccagctcta tattcagccg tcctgcctgc
     1141 aaaagaacca tggtaaggat aaacgcccat aaaagagcct gtgggaaggt atccggaagc
     1201 acagcctgcc agaaggcctc tctgttcgga accgcttttc tttcctgctg ctttctgctc
     1261 tcattttttc tcctgatttg gattgccgca cataccgcca gtgttcccgc agtaaggatc
     1321 agggccagaa tccgccagaa gggaactcct ccatggcctg taagggaaat cacgcagaca
     1381 atgaccatcc ataaggcgct tcctgttaca aggcccagag ccagagtctc tgcccagtgg
     1441 atattctccc attctttcct ggtcagatcc tgcatccttt tccggcctcc catcagccac
     1501 ccgttcagcc atcttcccag acagattaaa actgcaatcc acactcctga tataacagca
     1561 ggaacaagca tctgatgaca ccaggcaaac agaaggcttc ccacactgat tcccaaaatc
     1621 tgggctttac ggttctttac tctggaaagc cacagaaggt tccataaaaa tacggccagc
     1681 acctgggcca gcatggtctt tgtttcctct tttttcagta tctcccccag agaaccggtt
     1741 aaaaacagct caaatactcc gattcccagc acaatggccc cggctgccca gagcagaacc
     1801 aggaccattt tcatctctct tcgtctgatg ccgcacatcc tgaaacattt cctttactcg
     1861 tattccttat atgcagaggc atatccccgc atcaaaaacc ggaccaccgg ctccggccag
     1921 aaacgggcaa acatttcccg ttcctccttt gccctccggt tatcctccat acaggccttt
     1981 gtcgccgccc cgtcgtgaac ccgataatac ataagaggct tttcacggca gataaaacgg
     2041 cccggacgct ctgcaagctg caccagatta tcccagtcta acgcgtagct gtactcagaa
     2101 cgcacaagag gttctcccaa cagggacttg tggtaggtgg tcgcagggca gcagatggag
     2161 tttccaagca tcaaaggcag gcgttttacc caggtaaagc cattaaacgc aggacagcga
     2221 agaggcaggc gcaggatgcg tttcacccac aaaagcctgt ctcctgtaat ggtttttccg
     2281 ccctttgtaa tcacgtaatc cgttgtgaaa accgtcatat ccggccattt tccggctgcc
     2341 ttcaaaagct ctgccgcata ctccttatga tacacatcat cctgatgagc aatcgtcaca
     2401 tattccccat ctgccatttc ataggcataa ttccagtcat cccggatgct gctctctccc
     2461 cggcgcacat aaaggggcag ctcatacttc tctgccatct gctctaaaaa ttcactagga
     2521 gaagatgtac agaggataac cggcgacggc tggctctgag cctttaaaga gcggatacag
     2581 gtttccagcc agggcgactg cccataggcc ggaatggcaa acacgtgctt atacctcata
     2641 aaaatactcc tctaacatca ggcacagggc atgataaacc ggcagatgaa attcctgtat
     2701 cttaaaggtt tcctgctccg gaaccacaat acaggcatcc gtcatcgcct ttaaacggcc
     2761 gccatccctt cctgtaaggc caatgacctt taagcctgct gcttttgcca cagctgctgc
     2821 atacaggata ttctttgaat ttccggaggt agtaattcct aaaaatacgt ctccctttct
     2881 tccgtaacca tagacctgct gtgcaaagcc catgcttccg tccacatcat ttaaaaaggc
     2941 tgtggaaagg gcgggatgac ccgtaagggc aatggcagga agagccccct gaagccgctt
     3001 tgccagttcc tctccaagct ccggctctgc ctccttaagc ttctgttcaa aggcagaagt
     3061 caccggacgc cgttttacaa accccttcat cagttccccc acaatatgtt cgctgtccgc
     3121 acagcttccg ccgtttccgg ctatgagaag cgttccatcc tgcaaaaagg catccctgag
     3181 aatttcatat gtcttaaaaa tgtcctcttt tacaggcatc agtaccggat agcgctccat
     3241 caactcctta agaatcaaat ctaccttact gccgttcatc ctgttacctc tcttttttcc
     3301 tgaaatatat cacgtctctg ctttactgcc gccttaaaat ccagtccgca gcctggggca
     3361 gattctctgc ataataatca tatgagggcg cttcatcctt cttcgcccga ctggcttcat
     3421 aaagctcctt cccatatccc gttcctacca gaatggaggt cacaccgtag ttcttccccg
     3481 cctgcgtgtc taaaagctta tctccaatca tataggaatg ggacttatct gctgtaaacc
     3541 gctcttctgc ctgcaaaaac ataccgattc ccggtttccg gcagctgcac tcctttttat
     3601 agctcccgat tccgtgaacc ggatggtggg gacagtaata aaagccgtct atttcggccc
     3661 cctctgccgc cagcagctca ttcatatacc gatgaaggac ttttacatcc tcttccgtat
     3721 aatatcctct ggctaccccg gcctgattgg tgaccaccac cagcttaaat ccggcctcct
     3781 taagccttct caaagcttcc ggcaccccgg ctagcagatg catatcctcc ggatggtgca
     3841 gataatttac ctcttcattc agcgtgccgt cccggtccaa aaatacaatt ttttccatat
     3901 ctcattcctg cttacagatc aaaggtataa atcttatttg gcatatggac ctttacctga
     3961 tcgtacttcc agcgctcctc gtctgccacc cagctctggg ctccgcgcag ttcaaaattc
     4021 cagtctgcca gctggcctcc tgcctgctcc atacgggccg ctaccttgtg gcgcacattg
     4081 tatgggcagt acatgagaag ataaccgccg ccgccggctc caaggagctt tcctcccagg
     4141 gctccggcct tgatcgcctc gtcataaagc atatcaatct gaggattggt gatcttgctg
     4201 ctcatgcgct ttttgctttt ccatccatag tcaaggagtt ttccgaagct gtggaggttt
     4261 ccctttaaca gctcgtcctt catggcataa gccagggcct ttacctcaca catggcctca
     4321 aaggcatcct ttttctcgta attctgtacc tgatccctga taatgttggc agatacatgg
     4381 atatttcccg tatagcacag taaaaggttg tattgcagct catggataat gtcctttttg
     4441 attctcaagg gatttacaac tacgttgttt ctgccgtgga actcaataaa attaaagccg
     4501 ccgaaggccg ccgcatactg atcctgatag ccgccgtcaa tctttaaatc cagccgctcc
     4561 acctgatagg ccagatccgc catggcatag gcgtccatca tgatcccctt ccatcttgcc
     4621 atagccgtaa gcatggctac catcacagta gaggaggttc caaggcctga gccgggaggc
     4681 gcgtcgcact gtaaatatac ctcgcagccc tggctgatct ccatcgcctt aagagccgct
     4741 gttaccagat ccagacggcc gtcatagacg tagttttcct ttgtattgta ctttactgtc
     4801 atatcgaaat ccagagaatg gacaatgatc tgatcgtcct ctctgggtac gatagagcag
     4861 taggcatatt tattgatggt gctcccaata attgcgccgc cctgctcctc gcagaaaggg
     4921 gctacatccg tgccgcctcc gccgaagctg acccgcaggg gcgctctgcc tctgattacc
     4981 atatttttct ccttccgttt cttacctacc ggacaacgcc tgtttctaca tccctctgga
     5041 actggaaata agcctccgga ataccgatat caatgaaata accgtcattt acaaaaccgc
     5101 ccagccgctt tccctctgac agccagcagg gaatcctgtc attttccaga gatacctttc
     5161 cctccggaat ctcgtctaaa aggctttttt tcataaggta aataccgccg tttatggtgc
     5221 cggattttgc ctcctgtgtc ttttcattaa agcctgtaag gatgcccttt tcatctaaaa
     5281 cagcctgacc atagcgggat acgtccggca ctctgcgcag caccagcgcc atgtccagct
     5341 gctgctcatc ctgctgcctt accagtcttg tatagtcaat ctggtaaaag gtatctgcat
     5401 tgagaacgaa aaaacgatcc tctgtaatga attttccggc atttttaatg gcccctgcgg
     5461 tgcctaggag agtttcctca taggcgtaag ctgcatgaaa gccaaaatcg ctgccgtctt
     5521 taaaatattc ttccaccatg gagcccttat agcctacggc aaaaatgata tccctgacgc
     5581 cgtttcgcac cagttctctg gtaacgtact ccataaatgg tttttcctgt atgagagcca
     5641 ttggcttggg acggtcgctt actacgctgc gtaaacgggt gcccagacct ccggcgagaa
     5701 gtattgcctg cattttgttc tccttaaatg tatacttaaa tttctcagtg ctgacctcga
     5761 aaacgctgcg cgtttcctcg gtcgcgggca ttcgccctat agccatgcag ctttccccgt
     5821 cgaaaagtaa gcgagcttac ttttcaacgt gaaattctgc atggctgcac tgctcactgc
     5881 tgtcgcgaaa gtcctcctct atcaccttcc atgcgccttc gaggctgaag tgtctgcgga
     5941 tatagtcctg agtggcgtga cacatactcc ggcagcgggc aggatcgttg tagagagata
     6001 cacatttttc tgcaaatgca gccggatcat cctcgatttc cagtatggtt tctacctccg
     6061 gaattccttc ggctccggtg ccggttgtga caatgggggc tccgttgtag atggcttcga
     6121 ctaccttgcc ctttacgccg gccccgtacc gcaggggaac gactacgatt cggcaggagg
     6181 catacagctc cgccagctct tcttcgctga caaagccctt tatgataatt ccgtttccag
     6241 gctgctcaag ggcctttatc tcatccgtaa ccttggatcc taccacatag aagtttatct
     6301 ccggaagttt ttcccggatc agcgggaaga tctcctgtgc gaaccacagc actgcatccg
     6361 cattgggcgg atgagcaaat ccgcctacaa agagcagtcc ttctctcttt gcgaagtcct
     6421 cctggatatt ggcaagaaat tcatcgtata cataggctgt aatggccttt accgggattt
     6481 ccgggtcaat cttgtggatg gcatctacct ccacatagga cggataatag gaaatggccg
     6541 ccttccgcat catggacagc tcaatggctt tccagtaatc agcctcacgc ttgattgaaa
     6601 tatcgcctgt aagagcatac tcccgtccca gacgcaggaa atgaaggtca tggccgtaat
     6661 aaatcacttt gatattcgta ttttccttaa taaagtccac atattttgtg gcaatatggg
     6721 gccggtttaa ataggcaaaa tcaatttcat ccttattccg tttcagccag tcccagatac
     6781 cggaagccca gtcatccccg tagaggatct caattcccat ctgctggaga gccgtggaat
     6841 agggttcctc atggaggaag ttgtctccca ggaattttac cacatagccc tttttcagga
     6901 acattttcag atactgatag gtggtcttgg agcctgcgtc cttatcaaag gtaggcacat
     6961 agtgatccac taccaggatc acctttttac cctggcttct ctcccttgcc ctgaatggat
     7021 tgggatttcc gtcattgaca gactgacgct taaactcatc cgcccatttt tcccgaagct
     7081 ttttgctgtt ttccacctga taccgcttca ggcccgtacc gtttacatcc gtaccgttgg
     7141 acaccccttc aaaatgaatg actttggata gaggctgata taccacccgg tatccggctt
     7201 ttctcacctc aaaggccaga tcagaatctt cacagtaggc aggagcaaac cgctcgtcaa
     7261 agccgccgat ctgcttccag agctgaacgg ataaaaggat ggcggcgccg gaaatgtagt
     7321 ccacgtcctt tacatagttg tactctgcct tatccgggtc atccagccgc ccatagttcc
     7381 agccggagcc gtcgctccac agaatgcctc cggcctcctg aagacgtccg tccggataca
     7441 ccagcttgga gcctaccata ccgatagacg ggtcggattc gatcagcttc accagagaag
     7501 atagccaccc ttccgtcacc ttggtatcat tatttaaaaa catgatatat ttgcccctgg
     7561 ctgcctttgc cgcctggttg cagtttctta aaaagccctg attggtctga ttgcggcaga
     7621 tcaccagccc gtccacaaaa cggctgattt ccgccgttgc atctgttgac acatcatctg
     7681 caatgatgac ctcataggtc acgtccttcg tatattccag aatggactga agacaggcat
     7741 aggtgtagtg gatctggtta taacagggga tcacaatgga gactaagggc ccttcccagc
     7801 cattttttcc ctcttttccc tctcccggat actgcgggaa ggaaaggtgg cctccggtga
     7861 gatagccctc gccaatctta aaatcaccct caatcaggtt gcggccctct ttggtggcat
     7921 acatcttcat gtatcttgca gggtggcgca ccgtatggaa gcagtagttc aataccttgc
     7981 gctttctggt tccctttggg aatgccttat tgacctgaac acccagcttt ctgcgaagct
     8041 tgtatacagc cgcttcatga ccattcaaat aggtggcaag cttacccagc tcgtcaatct
     8101 catggcgcag atccttaatc atgatttcca ggttctttac atgattattg gtaaggcgca
     8161 gaatctcctg atcctttttc acctgaatat tgcgctcctc cacctcttcc tgaagctggt
     8221 tgatccgctc tctggcctga tccagttcct tttcctgggc ctttaattcc tctaaggtca
     8281 ccagacgctg tttataatta cccatatacc cctgagtgcc gtctccgtgt acccaggact
     8341 ggaaggattc ctccatggca gcatacagcc cggacagttc ttccccgatt ccgaattcct
     8401 tcaggaaatc agctgcattt tcatagtcca tcaggccttc atatttataa tagaaataaa
     8461 ccagattcct atagcggaca aaacctgccg gaacagggaa atcaaagacc cattcataat
     8521 ccaggcagta cagcttgccg ccgcttacca tcagattttc aaacaggccg tcaatattgc
     8581 tgactgcata ggaagcatcc gacagggcag acagctgctg ttccaattct cccgaagcgc
     8641 tgtcctgtga atcttctgcc ggattccttc caaacacctc cagaaattcc ggtgtcacat
     8701 aaaaggactc cgggcgcagg gctgcctttg agaagacctg ttccatcgct gcctgaattg
     8761 cctcaaccgg agccttcttt ccccggatct gcccgccaag ctcctctgcc agcgtctttc
     8821 ctgtaagaaa ctcaaaccgt accgcctgct tatcctcaga aaaatgcggt tccagcacac
     8881 gaacttcagg atttaccttg gagagcttct catgcttttc ctccatagaa ccaatatgtg
     8941 ctccggatgc ctctgtaaga gcagcctttt ccacatatcg ctttccattt tcttctaaaa
     9001 tgcgggtgcg gatctgaaac tgaggttttc tggtccggtt atattttgca aaaatcgtat
     9061 caaagggctc ctgctcctta gaggccactg ccagataggc attggcgaaa agtccaaagg
     9121 ccttttcctg aaccagctta tcggaaatct catcctcatg gatgcaggcc cagcgctggc
     9181 ggtcatagac tgcggtcaca tggggaatat cgccctttcc gggaagatac cggtcggaat
     9241 acaccgtctg agcccgctta tactccggca tgggataata aaattctacg gtttcaaggc
     9301 ctgcctcacg aaaagccgcc tccagttctc ccttggtaaa tgcactctca tcctcatcat
     9361 ggacagcacc agataaaaag cacagtccca gggcattttc acaggaaaac accagacggc
     9421 ctccctgttt tacatactga gaagcctcct ttacaacggc ctttaagtcc atgtccgggc
     9481 gggaagattc taaaaccaca agatcgtaag ctgctctgtg gtcttccttc ttccaatcga
     9541 tttcttttat ataggaaata ttgtccgcac tgctgtttct ctgctcattc accaccagat
     9601 tttcatccct gcggtccgtt acagtcacat gggcgcagcg ctctgcaaac aatccggtga
     9661 gaaccccata gccggaatcc agctgcagta cattttcaca ttttttcagc tccatccagt
     9721 cgaaaagatt tttccggaca tcggaaagag catacagcac ctccggacgg ctgtcatttt
     9781 caaggatttt ctgaatcccc tccggatcgg aaccgtattt tttaaagaga tccagaatct
     9841 catagcttat atcattcatc tgctttcctc ttttccgctt tcacctggga ttccatatcg
     9901 taaacgccca cagtgttttt attggaaata acagtaatat ttgtcacatc atacaaacgg
     9961 tgatataccg tatgctctcc ggcctcgaag ccggtacagc tcatgcttaa caggtactca
    10021 ccgccctgaa gggtcatctt ctgggtaaat tccaccacat attcctcgcc ctttttcacc
    10081 ggctgaatgt ccgctccctc aaacatggtg ttggtgccag tgagctccgt tcccttttta
    10141 tccttaatgg tataggtaaa aataggcgac tggatatccg catggaaacg gatgcattca
    10201 cgaatagtaa acatctctcc ttttaaaagg aggtttgtaa tattgcctct ggcgtctaaa
    10261 agacccagat catagatttc cgcccgtccg tcgccatatt ccgtacggtc tgaattgatc
    10321 gtcatctggt cgcgcatcag ccccttggga tgctgatttg ttgttccggc gctctcatgg
    10381 ttgtcaagaa atgttttttt tgccagctgt gcatcaacaa ggccgtctac gtccataccg
    10441 cctgagaaat cggaaaggtc atcctctgtg ccggacggct gatccgtttt atggactccg
    10501 gctgccagct ccgccttcag ctcctccagc tttccggcca gaatcttctt atacatatcc
    10561 accactttgc ccgcaggccc ttcagcgata aattcgccct tatgaagcag aaccacccgg
    10621 tcacagtatt tgataatgct tcccatatcg tgtgtaacca tcaggatggt cgtgcccttt
    10681 ttgcggatct cctccatccg gcggtagcat ttggactgga agaatacatc tcctaccgac
    10741 agagcctcat ctacgatcag aatttcagga tctacgttga tcgcaagagc aaaggccaga
    10801 cgaacaaaca taccgctgga ataggtcttt accggctgat agacaaaatc tccgatttca
    10861 gcaaaatcca gaatatcctg aagctttgca tccatctcct ttttcgtata gcccatcatg
    10921 gtgccgttca tgtagatatt ttcaattccc gtataatcca tattaaaacc ggctcccagc
    10981 tccaaaaggg cggaaatctt gccgtttaca tccacctgcc cggtggttgg cgtcagcact
    11041 ccggtgataa tctttaaaat ggtagacttt ccggaaccat ttgtaccgat gatccctacg
    11101 gtttccccct tctttacctg aaaggaaagt ccgcttaaag cataaaagtc cctgtgataa
    11161 tttttatggg aaatgctcat cgattccttt aaacggtcaa tgggcttgtc atacagccgg
    11221 tagaccttgg tcacgtcccg gacgctgatg gcattttcct gttctcttac ttccatatcg
    11281 ttctccatat ctagttgcat aactgcataa ttctccactt tagggataag tatatcatac
    11341 aatggcaaat ttttatactg gtaaaaagaa taaaaactac ccttcttcct cccccgccgc
    11401 cagcccgatc aacgttctgg cctcttttgt caaaaatttg ttcttatggt aaataacgga
    11461 aaacttccgt gacaatgcaa gtcctcttat aaagaactcc ttaacctgcc ccttctcaag
    11521 gctgtcccga accagataat aaggaagaag cgacaagccc agaccggctt ttaccccttc
    11581 cagcaccgcc tcattgctga cgctctccca taagggcttc acagaaattt cccctgctgc
    11641 cagaatcccg tccagaacct ctcgccccgc gctgccccgc tcccgcagga tcagatcctg
    11701 tcctgccagc tcttcggccg attttaaggt atgccctgca aacggatggg ccggagggca
    11761 gataaatacc agccgatcgc cgggaaaggt cctacagtgg atatagggac tgtgaaccat
    11821 tccctcaatg ataccgatat cgatctgatt ttcaagcagc ttctgctcaa tggcctctga
    11881 atttcccacc gccgcctgta cggtcagcga cggaaaacgt tccttcagct ccctgatata
    11941 tccgggaagc aggcaggagc cgatggtaat gctggtaccg atccgcaatt ctttcgccgc
    12001 ctggccacct cccaccccct gctccatctc gtcaaaaagc gcgataatat gtctggcata
    12061 ttccagggcc tgccgcccgc tgtctgtgag atacaaacgg cgtgaaatac ggtcaaagag
    12121 ccgaactttg taatgctcct ccatctctga aacagccaga ctgatagcgg gctgggcaat
    12181 atagagtatc tgggctgccg cagtcatgct tcctgtctcg cagaccgtga caaaaatcct
    12241 taaatgacgc agcgtcatga tccttctcct ttctttcact gattcgtata ttcataatat
    12301 tttcattata tatattataa tattatagta ttttacatat gtatctgcaa ggcgtatagt
    12361 aggaatgccg gatggatttt ccggcaaaat aaccgggaat acttcccgtt ttaaaaggag
    12421 ttcacccatg gaagctgtat cccatcatga aaagcggtct gtcatcttaa aaaagctttt
    12481 tatttctacc ttttatctca gcgcctttac ctttggcggc ggctatgtaa ttgtaacact
    12541 gatgaaaaaa acctttgtgg a
//
LOCUS       DBMDUH010000074        12423 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_2620_length_12423_cov_3.988801, whole genome shotgun sequence.
ACCESSION   DBMDUH010000074 DBMDUH010000000
VERSION     DBMDUH010000074.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 12423)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12423)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12423
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2620_length_12423_cov_3.988801"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="ACZPCG_07595"
     CDS             <1..437
                     /locus_tag="ACZPCG_07595"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1284967.1"
                     /translation="CQIPKLTLQPIVENSIYHGLEQKMEKGHLDIKITATDQHLIIMV
                     SDDGVGMEKEQVKKMNEVLRSLRLEEMQTESSRGGIAVRNVNNRIKLLFGEEYGISIY
                     SQSGAGTDVEITLPPNYDGGSYASEKVGGCNAEKQENIHFKP"
     gene            461..>1617
                     /locus_tag="ACZPCG_07600"
     CDS             461..>1617
                     /locus_tag="ACZPCG_07600"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1284968.1"
                     /translation="MKQEILRFENVTRQVDGAVYLDNFNFYMTKGEIVGMLSVNDRGK
                     KELLNLLMKRLPIESGRIYWDGGLANSWLDSEYPNNKVYVIDEHSSLIEDLSIADNMF
                     VMRSGFKKYVIEERVLEEQAKRVLESLSMQVDLNQRVSRLTVLERIMIEMAKAYLMGC
                     SLLIVMYPEQLIGQAEFERFHQLLRSIKKKGISTLYFCYHHWIMFQICDRIALFSRGR
                     IKKLFDHRDFTEKAIAPYIYYFKDARIARAGETPSYGLEFRELSGENIGRLNQGIVPG
                     ECITFLDSEGRMGRELLKILTKEKTDYEGRVFCKGIDLKKGGRSCLDSGMIVLDNNPT
                     ETFLFSEMSYLENLCFLLDRKLRKRILKPSYLSSVRNEYARRVGDVIDAGD"
     assembly_gap    1618..1627
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1628..1904
                     /locus_tag="ACZPCG_07605"
     CDS             <1628..1904
                     /locus_tag="ACZPCG_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284969.1"
                     /translation="EKYGLVYNKIRLFHPEILVILKPFAYGDMHCRNYILEQIRELKE
                     MGLCILLVTNYITDCLYISDHICILKDGRDVVSLEPEEYGIISRIFS"
     gene            2018..3322
                     /locus_tag="ACZPCG_07610"
     CDS             2018..3322
                     /locus_tag="ACZPCG_07610"
                     /EC_number="5.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018877.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HIR1284970.1"
                     /translation="MKPEKREKLQTVRNEMEAFMKRSVPFWLEHGIDREYGGYLVCFD
                     CHGRPMKELAVLSPDDKMIVTQTRMIWGFSALLRNGLAKRCGWEEACREAAVQGVDFF
                     IEKFWDKKHTGWAWVTDRKGNVLDNGKLVYGQTFAIYALAEYYMATGDARGLEYAEKT
                     FDALKKYAADTYHGGYFENFKEDWTLESPGVYGGDLKSLDIHMHTMEAYTTLYEATGK
                     EIHKRALEEIIQIVLTYMVDYDFWCGRNQFGSDFTPRPAISIRRTWNYDRDPESANKN
                     PLDTTSYGHNIELVWLLNRAYEVLGEPPAREFTRKFADYTLANGWDPIYGGIYRDGMH
                     DGRIVVTDKEWWQNFESLTGFLDCYQATGDERYLDTFIDLWEFDQKYFYNPEAGESRQ
                     LLKADGTPIIEDAGNQWKCIYHTDRAMMECCLRIEDMNRENE"
     gene            3344..4879
                     /locus_tag="ACZPCG_07615"
     CDS             3344..4879
                     /locus_tag="ACZPCG_07615"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007716405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR1284971.1"
                     /translation="MEEMNTHTPAGRELVRMSGIMKEFPGVRALDDVKLELYSGEVLA
                     LLGENGAGKSTLMKILSGVYQRDDGEIVLFGKKIQNWDANHAKEAGVAIIHQELNMCT
                     HLSVAENIFLGRELVKNGRLDNERMHEETKKILHRLNIDIDPRTQVGDLAVSRQQMVE
                     IAKALSQNARVLIMDEPTSALTSKEIDELFKIIHQLKEDGIGIIYISHRLEELQHIVD
                     RVIIMRDGKYITQMPYDVNRMDEIIANMVGHEIKEKFPHVDCTRGKKILEVRHLSAGR
                     QVRDVNLELYEGEIVGIAGLMGAGRTETTRAIFGVDSKEEGAIFIDGKEVKIHCPADA
                     IRAGLVLAPEDRKKDGLCTRLSISDNIALPNLDMLCKGLFGTVDRKKEKEMVDHSAKA
                     LKIRMSGAQVDAGSLSGGNQQKVVVAKWLARNSRVVIFDEPTRGIDVAAKVEIYNLMN
                     QLKQNGIGVMFVSSEMPEVLGMSDRILVMCDGRITGEFNASEATQDKILTSATRFEQK
                     AVS"
     gene            4893..5909
                     /locus_tag="ACZPCG_07620"
     CDS             4893..5909
                     /locus_tag="ACZPCG_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1284972.1"
                     /translation="MSNSNEKKPGILGRFLSQRGMGQVITVGVGLVILCLVFGFINPN
                     FFTYRNVANLLRQIAPILLIGVGQSYVLFTGNIDLSVGSVVGMSCMISATLMCHGMNP
                     WVSVVITMACCLAVGCVNGVLVSKCKLPPFIATLGTMTIARGIAQIVNGNYNTDSIGE
                     AAKGFRTFFYSGKFMGIFSAFWIAMAVWLVFNYLMSHTRTGRHIYATGSNIDAARLSG
                     VNVDNTVICVYVVSAFCACITGLITCASTGTGTMDAGTSYEMYAVAASVIGGVSTLGG
                     SGILSGTVIGAAIWGVLQNGLQFAGAPVAIRNIVIGIIVVVAVLLDVIVRSGKGKSKK
                     HKNG"
     gene            5976..7061
                     /locus_tag="ACZPCG_07625"
     CDS             5976..7061
                     /locus_tag="ACZPCG_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009428707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR1284973.1"
                     /translation="MRKKALSMILVAAMASAALAGCSSAPKETAAPETTAAETEKKET
                     DAKKEETTKAEETKKAEEAGGAANDMLAGETGDIAKPGDGKGQKVVLITIDSMDQHWV
                     NMDKGCKKAAEELGCEYKWIAPDVKDDAKQIECVNNAVADGATAILVAANGPDAITAA
                     LEEADEAGVKIVQVDSFANYPCVQKLGTDNRAAGKIAGEKVLEALEAKGVKEGKIGIV
                     SVNAATTSTVDRDEGFRSAFEGTAYEILETQYADGDAAKSKDAAANFISQGCVALFGA
                     NEGSCVGVGNAVAEDGNNIVAAGMDKSDAVIQLIKDGSLICTMAQNPDIMGYEGMYAA
                     ITAINGGSVAPEYIDTGVSVLTADKVQ"
     gene            7131..8042
                     /locus_tag="ACZPCG_07630"
     CDS             7131..8042
                     /locus_tag="ACZPCG_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR1284974.1"
                     /translation="MYVLGFDIGGTKSAVVLANIEDTQVSFLGRYERKTVPDWKKVLN
                     ELEENGRKIAKEHGISIGSNEGVERIGISCGGPLSSDRRQILSPPNLPGWDRVPVIDY
                     LEKKMGIPARLENDADAGALAEWRFGAGQGCRSMIFLTFGTGLGAGLILDGRLYRGAS
                     GLAGEAGHIRMEKDGPVGYQKSGSLEGFCSGGGIARMAEIEGIKASAKELAAMAEMGD
                     EKALKIFERSGAIFGRGLAVLIDLLNPERIIAGSVYARSHHFLDSAMYRELKKEALAP
                     SLAACRILPAALGERIGDYAAISAALE"
     gene            complement(8051..9316)
                     /locus_tag="ACZPCG_07635"
     CDS             complement(8051..9316)
                     /locus_tag="ACZPCG_07635"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HIR1284975.1"
                     /translation="MKHFTAAALSAVLSISIAAVSSPLSYWGAKENSREFSLFFSGPG
                     VEAVSSGDEYSEYQWALHNTGRLQRTERKLNIRTLDSIYVHYGENGVDGIALPPLGPS
                     NYDAIQTDAVAGIDINIKEAWQLYEQESNKRPVTVAVIDTGIDTSHKDLKDAIWVNED
                     EIPGDSIDNDGNGYVDDVNGWNFISNNNQLCAGDEDTHGTHGAGTIAAAKGNGGVAGI
                     GDSRYIKIMVLKALGGEEGKGSPENVIQAIRYAEANGAQICNLSFGSGQTSPEFEAAI
                     RDSNMLFVAAAGNGNQYEIGYNIDAHPVYPASFPYDNIITVGNLLFNGHLDESSNYGQ
                     INVDLAAPGTYILSTVPADTYAYMTGTSMAAPMVTGAAALLYSGRPDLSLSDVRTALL
                     SSVHKLAPLKGKTAYGGMLDVAAAMKWGR"
     gene            9496..10839
                     /locus_tag="ACZPCG_07640"
     CDS             9496..10839
                     /locus_tag="ACZPCG_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003505084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HIR1284976.1"
                     /translation="MGFTLDVSISAVTVFLQGLLSFFSPCVLPLLPLYIGYLSGGTAV
                     KGEDGRMHYKQSKVLLHTVFFVTGVSFAFFLLGLGVSAVGGFFHSYQAMFARTGGILV
                     MLFGLYQMGIFGISSVLGKEHRLPFRLDRFAMSPVTALLMGFTFSFAWTPCVGPALTS
                     VLLMAASAATKAQGFVLIGVYTMGFVLPFLFVGLFTTQLLELFKKCRGVVQYTVKIGG
                     ILMVLMGILMFTGKMNDVTGYLSRISGTQVSQTESMEESTVPEKAEADNEGGSTEPGT
                     GGSESTTAEEASGETAADARPVIPAVDFELEDQYGNIHRLEDYRGKTIFLNFWATWCP
                     PCKAEMPDIQKLYEKSSTEGEDAVIVLGVAAPNMGQEGSEEEIAAFMEEKGYTYPVLM
                     DTEGELFASYGIMSFPTTFMIDRDGNVFGYVSGMLSADMMDSIVRQTLDGKMDQR"
     gene            complement(10869..11435)
                     /locus_tag="ACZPCG_07645"
     CDS             complement(10869..11435)
                     /locus_tag="ACZPCG_07645"
                     /inference="COORDINATES: protein motif:HMM:NF024242.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR1284977.1"
                     /translation="MKQSDLQQYIHFRSTSDARKEIEPCLSLLLTELYENKIGSTHIC
                     HGLLLRIFRILSMHYDFSLTRERQKNINWTVFEEICSYIQTHYQSVTTHELMERFHFQ
                     RDYFNRLIRKKTGMTYSEYLQNIRLEQAKRLLIETELTISEISEQVGYHNKGYFYQLF
                     VNKYNMTPGKYKKRMLEHPCNPMPQHPE"
     gene            complement(11610..12005)
                     /locus_tag="ACZPCG_07650"
                     /pseudo
     CDS             complement(11610..12005)
                     /locus_tag="ACZPCG_07650"
                     /inference="COORDINATES: protein motif:HMM:NF019498.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12212..>12423
                     /locus_tag="ACZPCG_07655"
     CDS             12212..>12423
                     /locus_tag="ACZPCG_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013282198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HIR1284978.1"
                     /translation="MRIEDIKKRIQSGKTVLGIELGSTRIKGVLLTEEGELIADGSHQ
                     WENRLENGIWTYSMEAVWKGMQECYG"
BASE COUNT         3513 a         2181 c         3363 g         3356 t
ORIGIN      
        1 agtgtcagat tcctaagctg acgcttcagc cgattgtgga aaattctatt taccatggtc
       61 tggaacagaa aatggaaaaa ggtcatctgg atatcaaaat aactgctaca gaccaacact
      121 tgattattat ggtatcagat gatggtgtgg gaatggagaa ggaacaggta aaaaagatga
      181 atgaggttct gcgcagtctt cgcttggagg agatgcagac agagtcttcc agaggcggta
      241 ttgcggtccg aaatgtaaat aatcgaatta aactcctttt tggtgaagaa tatggaatta
      301 gcatatacag ccagtctggt gctggtacag atgtggaaat tacgcttccg cccaattatg
      361 acggcggttc ttacgcatct gaaaaggtgg gcggctgcaa tgcagaaaaa caggaaaata
      421 ttcactttaa accgtagtgg gtttgaggag gaaacaggtt atgaaacagg aaatactgcg
      481 ctttgaaaat gttacacggc aagtagatgg agctgtatat ctggataatt ttaattttta
      541 tatgactaag ggagaaattg tagggatgct gtctgtgaat gacagaggaa agaaagaact
      601 tttgaatctt ttaatgaaaa gactcccgat tgaatcagga cgtatttatt gggatggggg
      661 tttggcaaac agctggttag acagtgaata tcctaataat aaggtgtatg tgattgatga
      721 gcatagttct ttaattgaag atctttccat tgctgataat atgtttgtga tgcgttccgg
      781 ttttaaaaag tatgtgatag aagagagggt tcttgaggag caggcaaaaa gggttttgga
      841 gagtctgtct atgcaggtag atttgaatca aagagtatcc agacttactg ttctggagcg
      901 gatcatgatt gagatggcca aagcgtatct gatgggctgc agcctcttga ttgtaatgta
      961 cccggagcag ctgatcggtc aggcggagtt tgagcggttt catcagcttc tgcgaagtat
     1021 aaagaaaaag ggaatttcta cgctgtattt ttgttatcat cattggatta tgtttcagat
     1081 ctgcgatcgg attgctcttt tttccagagg acggataaag aagctgttcg atcatcggga
     1141 ctttacggaa aaagccattg caccatatat atattatttt aaagacgcca gaatagcccg
     1201 ggcaggagaa acaccttctt atggtctgga atttcgggaa ttatcgggag aaaatatcgg
     1261 gaggctgaat caggggattg ttcctggtga gtgcatcaca tttttagact ccgaaggacg
     1321 tatgggaaga gaactgctga aaattctcac gaaagaaaaa acggattatg aaggaagagt
     1381 tttctgtaaa ggaattgatt tgaaaaaagg gggaagaagc tgcctggaca gcgggatgat
     1441 cgtgttggat aacaatccaa cggaaacttt tcttttcagc gaaatgtcat atctggaaaa
     1501 tctctgcttt cttttggatc ggaaactgag aaaaaggatt ttaaagcctt cctatctttc
     1561 cagtgtcaga aatgagtatg cacggagagt gggagatgta attgatgcag gggatatnnn
     1621 nnnnnnnaga aaagtacggg ttagtttata ataaaatccg cctgtttcat ccggaaattc
     1681 ttgtaattct taagcctttt gcttatggag atatgcattg cagaaattat attttagagc
     1741 agattcgtga attaaaggaa atgggacttt gcattctgct tgttacgaat tatattacag
     1801 attgtctcta catttctgat catatatgta ttttaaaaga tggaagagat gttgtgtctc
     1861 tggagccgga agaatatggt attataagcc gaattttttc gtaagaatgt ctacctgatt
     1921 cgtaagttta tccccttatt aaaaaacagt cccctgttat aattatccca taaatatatg
     1981 gtatcctgcc atacagtatg agaagggggt atctggtttg aagccggaaa aaagagaaaa
     2041 acttcagaca gtcagaaatg aaatggaagc atttatgaaa agaagtgttc cgttctggct
     2101 ggagcatggg attgacagag aatacggagg gtatctggtc tgctttgact gccatggccg
     2161 tccgatgaag gaactggcgg ttttatcgcc ggatgacaag atgattgtga cccagacaag
     2221 gatgatatgg ggattttctg ctctgttaag aaatgggctg gcaaaacgct gcggctggga
     2281 agaggcctgc agggaggcag ccgttcaggg agtggacttt tttattgaga aattctggga
     2341 caaaaagcat acgggatggg cctgggtaac agaccgcaag ggaaatgtgc tggataacgg
     2401 caagctggtg tatggtcaga cctttgcaat ctatgcgctg gcagagtatt atatggcaac
     2461 gggtgatgca cgcggactgg aatatgcaga aaaaaccttt gatgcattaa agaagtatgc
     2521 agcggatacg tatcatggag ggtattttga gaattttaaa gaagactgga cattagaaag
     2581 tccgggtgta tatggaggcg atcttaagtc tctggatatc catatgcaca ccatggaagc
     2641 ttatacgact ctttatgaag caacaggaaa ggaaattcat aagagggctc ttgaggaaat
     2701 catccagata gttcttactt atatggtaga ttacgatttc tggtgcggac gaaatcagtt
     2761 tggttcagat tttaccccca gacctgcaat ttctattcgg agaacatgga attatgacag
     2821 agatccggaa agtgccaaca aaaatcctct ggatactacc tcctatggac acaatattga
     2881 gctggtctgg cttttaaatc gtgcgtatga ggtattggga gaaccgcctg ccagagaatt
     2941 tacaagaaaa tttgcggatt atacgctggc aaacggctgg gatccaatct atggaggcat
     3001 ttatcgggat ggaatgcatg atggacggat tgtagtgacg gataaggaat ggtggcagaa
     3061 ctttgagtcc cttacaggct ttttagactg ctatcaggca acgggggatg agcggtatct
     3121 ggatactttt atagacctgt gggaatttga ccagaagtat ttttataatc cggaagcagg
     3181 ggaatccaga cagcttttaa aagcagatgg aacgccgatt attgaagatg cagggaacca
     3241 gtggaaatgc atttaccata cagaccgggc tatgatggaa tgctgtctca gaatagagga
     3301 tatgaaccgg gaaaatgagt aagagcaccg gaaaggaacg aatatggaag aaatgaacac
     3361 acatacgccg gcgggccgtg aactggtgcg tatgagcggg attatgaagg aatttccggg
     3421 cgtaagggcg ttggatgatg taaagctgga attgtattca ggagaggttc tggcgctttt
     3481 aggagaaaac ggagcgggaa agtctaccct gatgaagatt ctgtcgggag tttaccagag
     3541 agatgacggg gaaatcgttc tgtttgggaa aaaaatccag aattgggatg caaaccatgc
     3601 aaaagaggct ggtgttgcaa ttatccatca ggaactgaat atgtgtaccc atttaagtgt
     3661 tgctgagaat atttttctgg gccgtgaact ggtcaaaaac ggacgcctgg acaatgaaag
     3721 gatgcatgag gagacaaaga agatccttca tcgtctgaat attgacatcg atcccagaac
     3781 tcaggtggga gatctggcgg tatccagaca gcagatggta gaaattgcaa aggctctttc
     3841 tcagaatgca agggtactga ttatggatga gcctacatca gctctgacat ccaaagagat
     3901 tgatgagttg tttaagatta ttcatcagtt aaaagaagat ggaatcggca ttatctatat
     3961 ttcccaccgt ttggaggaac ttcagcatat tgtggatcgt gtgattatca tgcgggatgg
     4021 aaaatatatt acgcagatgc cttatgatgt aaaccggatg gatgagatca tagccaatat
     4081 ggtaggccat gagattaaag agaaatttcc tcatgtggac tgtacaagag gaaagaaaat
     4141 tttagaggtc cgtcatctga gcgcgggacg ccaggtaagg gatgtaaatc tggaattgta
     4201 tgagggtgag attgtcggga ttgcaggcct tatgggtgca ggccgtacag agactacgcg
     4261 ggcaattttt ggtgtagatt ccaaggaaga aggagcaatt ttcattgacg gcaaagaagt
     4321 aaagatacac tgtccggcag atgccatacg ggctgggctt gtactggcgc ctgaggatcg
     4381 gaaaaaggat ggactatgca ccagattgag catttctgat aatattgctc tgccgaatct
     4441 ggatatgctc tgtaaaggct tgtttggaac cgtagaccgg aagaaggaaa aagagatggt
     4501 ggatcattct gcaaaggcat taaagatccg tatgtctggg gcgcaggtgg atgcaggaag
     4561 cctttcagga ggaaaccagc agaaggtagt agtggctaaa tggctggcaa gaaattcaag
     4621 ggttgttatt tttgatgagc caacaagagg aattgatgtg gcggccaagg tcgaaattta
     4681 taatctgatg aatcagttaa agcaaaatgg tataggcgtt atgtttgtgt cctctgagat
     4741 gccggaggtt ctgggaatga gcgaccgaat cctcgttatg tgtgacggac ggatcactgg
     4801 tgaatttaat gcatcggaag cgactcagga taagatcctt accagcgcta cgcggtttga
     4861 acaaaaagca gtgtcataag gaggaggaac acatgagtaa ttccaatgaa aaaaagcctg
     4921 gtatactggg acgctttctg tcccaacggg gaatgggaca ggtcattaca gtgggagtag
     4981 gcctggttat cctctgtctg gtatttggct ttatcaatcc aaatttcttt acatacagaa
     5041 atgtggcaaa tctgctgaga cagattgctc cgattttgtt aatcggtgta ggacaatcct
     5101 atgttctttt tacaggaaat attgaccttt cggtcggttc tgtcgtagga atgagctgta
     5161 tgatttctgc tacactgatg tgtcatggca tgaatccctg ggtttctgtt gtgattacaa
     5221 tggcatgttg tctggcagta ggctgtgtca atggagttct ggtatcaaaa tgcaagctgc
     5281 cgccgtttat tgccacatta ggtaccatga cgattgcccg cggaattgcc cagatcgtaa
     5341 atggaaatta caatacagat tccattggtg aggcggcaaa gggcttcaga acctttttct
     5401 acagcggcaa attcatgggg attttcagtg cattctggat tgcgatggca gtatggctgg
     5461 tatttaacta tctgatgagc cataccagaa caggccgcca tatctatgcg acaggaagta
     5521 atattgatgc tgcaagactt tctggtgtaa acgtagataa tacggtcatc tgtgtttatg
     5581 tggtatctgc gttctgtgcc tgcattacag gcttgattac ctgtgcatcc acaggaacag
     5641 gaaccatgga tgcaggaact tcctatgaaa tgtatgcggt agctgcttct gtaattggcg
     5701 gtgtttctac gctgggcggt tctggaattc tttctggtac ggtgatcggt gcagcaatct
     5761 ggggtgtgct gcagaatggt cttcagtttg ccggtgctcc ggttgcaatc agaaatattg
     5821 tgattggaat tattgttgta gtagcagtgc ttctggatgt aattgtccgt tctggaaaag
     5881 gaaaaagcaa gaagcataaa aatggataag cagtaaaata gggttgaaag cagaaatgct
     5941 ttacaataag agaaagaaag gaaaggtatt gtattatgag gaaaaaggca ttatcaatga
     6001 ttctggtggc agctatggca tctgcagcat tagcgggctg ttcatctgct ccaaaggaga
     6061 cagctgcacc ggaaacaacg gcagcagaga cagagaagaa agagacagat gcaaagaagg
     6121 aagagacaac caaagcagag gaaaccaaaa aagcagagga agcaggcggg gctgcgaatg
     6181 atatgctggc tggagagaca ggagatattg caaagcctgg agacggaaaa ggccagaagg
     6241 ttgttctgat taccattgac tctatggatc aacattgggt aaatatggat aaaggatgca
     6301 aaaaggccgc agaagagctg ggatgtgaat ataagtggat tgctcctgat gtaaaggatg
     6361 atgcaaagca gattgaatgt gtgaataatg ctgtagcgga tggtgctact gcaattctgg
     6421 tagcagccaa tggaccggat gcaattacag cggctcttga agaagcggat gaagcaggcg
     6481 taaagattgt tcaggttgac tcttttgcaa attatccatg tgtacagaag ctgggaaccg
     6541 ataatcgtgc tgcaggaaag atcgcaggcg agaaggttct ggaggctctg gaggcaaagg
     6601 gtgtgaagga aggaaaaatt ggcattgtat ctgtaaatgc agctactact tctactgtag
     6661 atcgtgatga aggcttccga tccgcttttg agggaactgc ttacgaaatt cttgaaactc
     6721 agtatgcaga cggcgatgca gcaaaatcca aggatgcggc agctaatttc atttctcagg
     6781 gctgtgttgc cttatttggt gcaaatgaag gctcttgtgt aggcgttgga aatgcagttg
     6841 cagaggatgg aaataatatt gttgcagctg gtatggataa gtccgacgca gtaattcagc
     6901 tgattaaaga cggctcctta atctgtacaa tggctcagaa tcctgatatt atgggatatg
     6961 agggaatgta tgcagctatt acagcaatta acggcggcag tgttgccccg gaatatattg
     7021 atacaggcgt ttccgtactg acagctgata aggttcagta aaaggaaaat gaaatttgaa
     7081 aaagatgctc ccggtatttc cgggggcatt tttgggtagg aggatacagg atgtatgtgt
     7141 taggatttga cattggagga acaaagtctg cggttgttct ggcaaatatt gaagatactc
     7201 aggtaagttt tctgggaagg tatgaaagaa agacggttcc agattggaag aaagtattga
     7261 atgagttgga agaaaatgga agaaaaatag caaaagagca tgggatttct attggatcga
     7321 atgaaggagt ggaacgaatt ggaatttctt gtggaggccc tttaagttca gatcgcaggc
     7381 agattctttc gccgcctaac cttccgggct gggatcgggt tccagtaata gattatctgg
     7441 agaaaaaaat ggggattcca gcacgtttgg aaaatgatgc agacgcaggg gcattggctg
     7501 agtggagatt cggagccgga cagggctgcc gcagcatgat ttttctgact ttcggaaccg
     7561 gtttgggagc tggccttatt ttagacggca gattatatcg gggagcatct ggtctggcag
     7621 gagaagcagg acatatacgg atggaaaagg atgggccggt gggatatcag aaatccggaa
     7681 gcctggaggg cttctgttca ggaggaggaa ttgccaggat ggcagagata gaagggataa
     7741 aagcaagtgc aaaagaactg gctgctatgg cagaaatggg agatgagaaa gctttgaaaa
     7801 tttttgaacg ctctggggcc atatttggaa gagggcttgc agttttaatt gatttgctga
     7861 atccggagcg tattattgca ggctctgtgt atgccagaag ccatcatttt ctggattcag
     7921 ctatgtatag agaactaaaa aaggaggctt tggctcccag tctggcagcc tgcaggattc
     7981 tgccggctgc actgggagag cgaatcgggg attatgctgc catcagtgcg gctttggaat
     8041 aaaaggatat ttatctcccc cacttcatag ccgccgccac atccagcata ccgccgtaag
     8101 cagtctttcc ctttaaggga gccagcttgt ggactgagga cagcagggcg gttctcacat
     8161 cggaaaggct gagatccgga cggccggagt agagaagagc cgctgcgcct gtgaccatgg
     8221 gagcagccat ggaggtaccg gtcatatagg cgtaggtatc ggctggtacg gtgctcagga
     8281 tataggttcc gggagcagcc agatctacgt ttatctgtcc gtagttggag ctttcatcca
     8341 gatgaccgtt aaaaagcagg tttcctacag tgataatatt atcataaggg aagcttgcgg
     8401 ggtaaaccgg atgtgcgtca atgttatagc cgatttcgta ctgattgccg tttccggctg
     8461 cggctacaaa aagcatattg gaatcccgga ttgcagcctc aaattctggg gaggtctgtc
     8521 cggagccgaa gcttaaattg cagatctggg cgccgttggc ctctgcatag cggattgcct
     8581 ggatgacgtt ttcgggagaa ccctttcctt cttcgcctcc cagagccttt aataccatga
     8641 tttttatata ccggctgtct ccgatgccag ctactccgcc gtttcccttt gcagccgcaa
     8701 tggtaccggc cccatgggtg ccgtgggtat cctcatcgcc tgcgcagagc tgattattat
     8761 ttgaaataaa attccagccg tttacatcgt caacataacc atttccgtcg ttgtcaatgg
     8821 aatctccggg gatttcatcc tcattgaccc agatggcgtc ttttaaatcc ttatgggagg
     8881 tgtcgattcc tgtgtcgatt acagccacag tgacagggcg tttattggat tcctgctcat
     8941 agagctgcca ggcttctttt atgttgatat caattccagc tacagcgtct gtctgaatgg
     9001 cgtcatagtt actggggcca agaggcggca gggcgatgcc gtcgactccg ttttcgccat
     9061 agtgtacata gatgctgtct aaggttctga tatttagttt tcgctcagtt ctctgcagac
     9121 ggcctgtatt atgcagtgcc cactgatatt ccgaatattc atcacctgaa gaaacagctt
     9181 ctacgccggg tccactgaaa aaaagagaaa attcccggct gttttccttt gctccccaat
     9241 aggaaagagg ggaggaaacg gctgctatag aaatggaaag caccgctgaa agggcggctg
     9301 ccgtaaaatg cttcatatct gaaatctcct ttcttgaggc tgtattatac catattttta
     9361 agatatatgc caagatgggt tcatctgaac attttgtgaa aaattcgcag aagcataagt
     9421 ctgtgacaag ctcacttgac aaaatggcag aaatcagagt ataaaaggag tacactttag
     9481 gattggaggt tatttatggg atttacactg gatgtaagca tatctgcggt aactgtattt
     9541 ttacagggat tgttaagttt tttttcgccc tgcgtactgc cgctgcttcc cctttatatc
     9601 ggctatctgt ccggcggaac agccgtaaag ggcgaagatg gaagaatgca ctataaacag
     9661 agcaaggtgc tgcttcacac ggttttcttt gtgacagggg tcagctttgc ctttttcctg
     9721 ctggggcttg gcgtgtccgc agtgggtgga tttttccaca gctatcaggc catgtttgca
     9781 cggaccggag gtatccttgt catgctgttt ggcttgtatc agatgggaat atttggaata
     9841 tcttccgttt tgggaaaaga gcaccgcctg cccttcaggc tggatcgttt tgccatgtcg
     9901 ccggttacag cgctgcttat gggctttact ttcagctttg cctggacgcc ctgcgtagga
     9961 ccggctctta caagcgtcct tttgatggcc gcatctgcag cgaccaaggc tcagggcttt
    10021 gttctgatcg gcgtttacac tatgggcttt gttctgccct ttctgtttgt gggcctcttt
    10081 actactcagc ttctggagct ctttaagaaa tgcagaggtg tggtgcagta tacagtaaaa
    10141 atcggcggta tcctcatggt gctgatggga atcctcatgt ttaccgggaa aatgaatgat
    10201 gtgaccggat atttatcccg tatttccgga actcaggtga gtcagacaga gagtatggaa
    10261 gaaagcactg tgccggagaa agcagaagca gataacgaag gcggaagtac agagcccggg
    10321 acaggcgggt ctgaaagcac aactgcagag gaggcttccg gtgaaacggc agccgatgcg
    10381 cgtcccgtga tcccggcagt tgattttgaa ctggaggatc agtacggaaa tatccacaga
    10441 ctggaggatt acagagggaa gacgattttt ctgaacttct gggcaacctg gtgtccgccc
    10501 tgtaaggctg agatgccgga tattcagaag ctgtatgaaa agtcctccac agagggagag
    10561 gatgcagtaa ttgttctggg cgttgctgcg cccaatatgg gacaggaagg ctctgaggaa
    10621 gaaattgccg cattcatgga agaaaaagga tatacttacc cggtactgat ggatacagag
    10681 ggagagctgt ttgcctctta tggtatcatg tccttcccaa ccacctttat gattgacaga
    10741 gatggaaatg tatttggata tgtgagcggt atgctgtcag cggatatgat ggacagcatt
    10801 gtgcgtcaga cgctggacgg gaagatggat cagagataaa atatctgcgg gaaaagtata
    10861 tacagaagct attctggatg ttggggcata ggattacagg gatgctccag catccttttt
    10921 ttgtattttc caggcgtcat attatatttg tttacaaaaa gctggtagaa atagccttta
    10981 ttatggtagc ctacctgttc ggagatttcg gatattgtta attctgtttc aatcagaaga
    11041 cgttttgcct gttccagacg gatgttttga agatattccg aataggtcat ccctgttttt
    11101 ttgcggatca gccggttgaa ataatctctt tgaaaatgaa accgttccat cagttcatgg
    11161 gttgtgacgg attgatagtg ggtctggata tagctgcata tttcttcaaa gacagtccag
    11221 tttatatttt tttgtcgttc tctggtaagg gaaaaatcat aatgcatgct taaaattctg
    11281 aaaatacgca gaagaagccc gtggcagatg tgggtggaac cgattttatt ttcatacagt
    11341 tctgtcagga ggagggaaag gcagggctcg atctcttttc tggcatcact tgtggagcgg
    11401 aaatgaatat actgctgcag atcgctttgt ttcatcagtg cagtttgaag aaaggatata
    11461 attttgccgg ttgtaatatg ttcattcatt acctcttcaa atatgctgtt ttcaattccg
    11521 aaaaacagta caatggaatc ctggcaggac agataatcct gatggataca gtttttgtca
    11581 atcaggcaga cgtcaccttc ctgaaatata atatctttcc cggaaatttt ctgtttgaag
    11641 ttacctttta caacgtatcc cagttcaatg tagtcatggg tgtggagctg cgtttttact
    11701 ccgtcttcaa atgtaaagca gaagcagaac ctatcaggtg cgggatacat ggcggcacga
    11761 agctgatttc ttccgcagaa atcccagcac aggaagaaaa tccgttggga agaaagcagg
    11821 ggatatgttt tgatttcttt tgcggatttg gaatattcga ctgaaatcat tctggctcca
    11881 agaggatgac cgggctgcag gttcctccgg gcagcggata taagctgtct gcatgtttct
    11941 gtcagattca aaattttcac ctcgcatttt gtgttgattt tctgaatgtt tgattctgat
    12001 atcataaaat tatatcgtat tttggaaaaa taacaatgaa aaatgatcga aataggtaac
    12061 tttagaataa gagtggcaag attatggaat tgtaccgctg atccctgttt tgtaaaatag
    12121 aatcagatct ttataatttt atgcaataga tgaaatgaaa cgtatgatat tttacagata
    12181 accgttaaag gagagcagaa aattggaaga aatcaggata gaggacataa aaaaacggat
    12241 tcagtcagga aaaacggttt tgggaattga acttggttcc accagaatca agggcgttct
    12301 tctgacagaa gagggagaat tgatagcgga tggcagccac cagtgggaaa acaggctgga
    12361 aaacgggata tggacctatt cgatggaagc agtctggaag ggaatgcagg agtgctatgg
    12421 cga
//
LOCUS       DBMDUH010000075        12010 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_2712_length_12010_cov_5.930137, whole genome shotgun sequence.
ACCESSION   DBMDUH010000075 DBMDUH010000000
VERSION     DBMDUH010000075.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 12010)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12010)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12010
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2712_length_12010_cov_5.930137"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="ACZPCG_07660"
     CDS             <1..461
                     /locus_tag="ACZPCG_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284979.1"
                     /translation="VYIPDEAVRAADVEAVRYFEDHIEAKNVLEIRARNKRKGEILYK
                     LRTTGKIRGIPYQIYFNSCNLEHVLYGELKDFTDEEKEIRSDNFADLYEGRAEAFIEF
                     ISGLAVAVPGTYKDTWNYIEKNCNSLNRHSNMHLLFEQNGASSQFTVAAP"
     gene            complement(442..783)
                     /locus_tag="ACZPCG_07665"
     CDS             complement(442..783)
                     /locus_tag="ACZPCG_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2-like protein"
                     /protein_id="HIR1284980.1"
                     /translation="MTEHSAIERYIEALETKDFKALGDLFTSDGHYCDYCPNGTSQHE
                     YHLYGKEAITMFFQNKFLFRQYSILSPVILNDRQAEFIASFGGCYVMAIANLQQLSAD
                     GHIRRLTVRPR"
     gene            complement(819..1610)
                     /locus_tag="ACZPCG_07670"
     CDS             complement(819..1610)
                     /locus_tag="ACZPCG_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012740842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR1284981.1"
                     /translation="MGIYAVTGGSNGIGAKTISILKQKGHETINIDWKRGDIEADLSL
                     PEGRQKAIDELFSLCPQGLDGLILCAGIPGSRKDLRLILSVNYFGTISIIKGAYDLLK
                     QKNGACVISASSAISREDMKTDLVDLLNNNRDDEYRILEVIGRMDGADLNTGDQIYAA
                     SKYALCRWMRRHSASYAANGVRINAVAPGNVNTPMTATGSKDTAAALNALPIPTKYGT
                     KKQMDPEEIASVITFLISPEARGVNGIVMFVDGGTDALLNTEKVY"
     gene            1710..3107
                     /gene="pdxR"
                     /locus_tag="ACZPCG_07675"
     CDS             1710..3107
                     /gene="pdxR"
                     /locus_tag="ACZPCG_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008975908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070279 - vitamin B6 binding [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:2000142 - regulation of DNA-templated
                     transcription initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MocR-like pyridoxine biosynthesis transcription
                     factor PdxR"
                     /protein_id="HIR1284982.1"
                     /translation="MLHYELVKKRGCPLYECLYECLREDILRGRLLPGTKLPSRRELA
                     RDNGISVKTVVNAYEQLLVEGYLIAKEKKGYFVADVETMDQYHPEKAEYPQLYREEKW
                     FADFTANNTIYEKFPFSMWRKVMREVLSEYDMELVQRAHFLGVRQLREVIADYLYRLR
                     GIVVSPECIVIGAGIEYLYGKLVKLLPGDSIYGVENPGYKKIPRIYEEYGLSWRYTEM
                     DENGISMESLRESGADVIHVSPEHHYPLGTVMSAARRQELLNWAVEKEGRYVIEDDYD
                     CEFRYRSRSIPALKSMDRNGRVIYMNTFSKTLAPAIRISYMVLPEALISRYIDNANFY
                     SNTASACEQYALAKFMEKGYFERHLSRLKKYYHTEGERLIRIIRQSSLLPVADVRGGE
                     SGTHLLVKLDTSLTDGQIREKAKQQGILVACLSQFCTRVRPEYERVLVLNYSDMDEGR
                     LREAVRRLGNVFMQW"
     gene            3226..4308
                     /locus_tag="ACZPCG_07680"
                     /pseudo
     CDS             3226..4308
                     /locus_tag="ACZPCG_07680"
                     /inference="COORDINATES: protein motif:HMM:NF051259.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
     gene            4325..5812
                     /locus_tag="ACZPCG_07685"
     CDS             4325..5812
                     /locus_tag="ACZPCG_07685"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR1284983.1"
                     /translation="MKKAELQSKYQLLIGGQWKDASDGKLFKTTCPADGSFLAECAEA
                     TREDVDEAVAAAWKAFESWKNTTVAERAAVLNKIADIIDANKEHLAMVESMDNGKPIR
                     ETLAVDVPMAADHFRYFAGCILAEEGSANMLGNTTLSLILREPIGVVGQIVPWNFPFL
                     MAAWKLAPVLASGCCSVFKPSSTTSLSVLEFARLVQDVIPAGVFNVITGSGSRSGQYM
                     LEHKGFRKLAFTGSTEVGRSVAMAAAEKLIPSTLELGGKSANIFFEDCDWEMAMDGLQ
                     MGILFNQGQVCCAGSRVFVQESIYDRFVEEAVKRFNQVKVGLPWEEDTQMGSQIDRRQ
                     MEKILKYVEIGKEEGAKLLCGGVQAKEGELEKGCFLRPTLLGDVTNHMRVAQEEIFGP
                     VACILKFKTEEEVIAMANDSEYGLGGAVWTRDINRALRVARGVETGRMWVNTYNAIPE
                     GAPFGGYKQSGIGRETHKVILDHYTQMKNIMINLAEVPTGFYPAK"
     gene            complement(5891..6403)
                     /locus_tag="ACZPCG_07690"
     CDS             complement(5891..6403)
                     /locus_tag="ACZPCG_07690"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1284984.1"
                     /translation="MDLQLVKMGPEYREMAEDMLREWKASGEAIIPSAIDQADIHDFP
                     AYLDSLELTEAVPGLVPDSTFFCLDKERNILVGAINIRHYLNDHLLKFGGHIGDGVRP
                     SERRKGIATRMIALGLEECKKLGITRVLMTCKKENTGSAKSIMNNGGILENEIEKEDG
                     TVMQRYWITL"
     gene            complement(6403..7695)
                     /gene="aroA"
                     /locus_tag="ACZPCG_07695"
     CDS             complement(6403..7695)
                     /gene="aroA"
                     /locus_tag="ACZPCG_07695"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HIR1284985.1"
                     /translation="MELKKWGPLRGEITVPGDKSISHRAVMFGSLAKGTTEIHGFLQG
                     ADCLSTISCFEKMGVSIENKGDVVLVHGNGLHGLKKPETILDCGNSGTTTRLISGILS
                     AQDFDVTLTGDASIQKRPMKRIMEPLSRMGADITSIRGNDCAPLLIRGTKLHEIHYTS
                     KVASAQVKSAILLAGLYADGITQVTEPFVSRNHSERMLKLFGASVSTEGTTASISPAT
                     QLYGQKVMVPGDISSAAYFIAAGLMVPGSEILIKNVGINPTRDGLLHVCRAMGADLTL
                     LNVRGQEGEPTADILVRHSALHGTTVGGAIIPTLIDELPMIAAMAAAAEGETRICDAG
                     ELKVKESNRIAVMVENLTAMGADVTETEDGMIIRGGKPLHGAVINSHLDHRIAMTFAV
                     TAQCAEGLTTIQGADCVNISYPRFYEELMGLIQKRGVC"
     gene            complement(7717..8847)
                     /locus_tag="ACZPCG_07700"
     CDS             complement(7717..8847)
                     /locus_tag="ACZPCG_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0033730 - arogenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - L-tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HIR1284986.1"
                     /translation="MEQFTIKDSTIAFIGLGLIGGSLARGLKRARPDIRIMAYMRTRS
                     KLEQAKKDGIIDVILNGIGEELLECDIIFLCTPVEYNAQYLSAIRPYLKPGALITDVG
                     STKTDIHKEILRQGLSHCFVGGHPMAGSEKTGYEHSTDHLLENAYYIVTPPEGLTEPS
                     ASYSDNVCRLLAVAQAVGAIPLVLDYREHDRVTAAISHLPHLVASSLVNLVKDSDSPE
                     GIMRRVAAGGFKDITRIASCSPEMWEQICMTNAGPIGDMLEQYIGSLQDILDQIRGHK
                     DKEIYRLFEKSRTYRDSITDRAKGPIEPSYEFSVDVVDEVGSISTIAVILAAKGISIK
                     NIGINNNREHGEGALKIAFYDEASMKEAWERLEKYKFELVRL"
     gene            complement(8882..9901)
                     /gene="aroF"
                     /locus_tag="ACZPCG_07705"
     CDS             complement(8882..9901)
                     /gene="aroF"
                     /locus_tag="ACZPCG_07705"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIR1284987.1"
                     /translation="MIIIMKPDASKEAVKKVTDLIEAKGLQPHLSEGSQVTIVGVVGD
                     KTKLMGSNIEMSEGVDKVVSVTESYKLVNKKFHPDDSVIPVGNTHIGPGTLTVMAGPC
                     AIESREQLMETAFAVKKAGATFLRGGAYKPRTSPYSFQGLEEEGLKYMKEAREATGLN
                     VICEVTSAHAIEAASKYVDMLQIGARNMQNFELLKEAGKSGMPVLLKRGLCATIDEWL
                     NAAEYIISEGNPNIVLCERGIRTYETSTRNTLDISAVPVIRQKSHLPIIVDPSHATGV
                     RAYVEPLAKAAIAVGADGLMIEVHPNPACALSDGPQSLTFDAFEKLMADLKPYCDLAG
                     RTVTR"
     gene            10230..>12010
                     /locus_tag="ACZPCG_07710"
     CDS             10230..>12010
                     /locus_tag="ACZPCG_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIR1284988.1"
                     /translation="MNVYGTEQIRNVVLLGHGGAGKTTVAEALAFITGVTKRMGKVPD
                     GNTISDYDKEEIKRQFSISTTLIPLEYEGENGPVKINLLDTPGFFDFVGEAEEAISVA
                     DAAIIVVNCKAGIEPGTERAWEMCEKYNLPRLIFVTNMDDDHASYRELVVKLEKKFGR
                     KIAPFQLPIRENEKFVGFVNVVKMAGRRFTTLSDYEECEIPDYVQKNLTIARDALIEA
                     VAETSEEYMERYFLGEEFTQEEISQALRTHVIEGDIVPVMMGSGINCQGFKVLLQAMD
                     KYFPSPDHFECIGVDVSTGERFTAKYNDDVSLSARVFKTIVDPFIGKYSLMKICTGTL
                     KGDTTVYNVNKDAEEKIGKLYVLRGKDVIEVSELKAGDIGAVAKLTVTQTGDTMAVRT
                     APIVYHKPQISTPYTYMAYKAVNKGEDDKVSTALARMMEEDLTLIVKNDAENRQSLIY
                     GIGDQQLDVVVSKLQSRYKVAVELSKPKFAFRETLRKKVKVQGKHKKQSGGHGQYGDV
                     IMEFEPSGDLETPYVFEEKVFGGSVPKNYFPAVEKGLQECVQKGPLAGYPVVGLKATL
                     LDGSYHPVDSSEMAFKMATILAFKQGFV"
BASE COUNT         3080 a         2717 c         3193 g         3020 t
ORIGIN      
        1 gcgtgtatat tccggatgaa gcggtaagag ctgccgatgt ggaagcagtc cgatatttcg
       61 aggatcatat agaagctaaa aatgttttgg aaataagagc acgcaataaa agaaaagggg
      121 aaatccttta caagctgagg acaacaggaa aaattcgcgg tatcccttat cagatttatt
      181 tcaattcctg taatttagaa catgtcctgt atggtgaact gaaagatttt acggatgaag
      241 agaaagaaat acgatccgat aattttgctg atctgtatga gggaagggca gaggcgttta
      301 tagagttcat ttccggcctg gcagtagctg ttcccggcac atataaggat acctggaact
      361 atatcgaaaa gaactgtaac tccttaaatc ggcattccaa tatgcatctg ttatttgagc
      421 aaaacggtgc ttccagccag ttcaccgtgg ccgcaccgta agccgtctga tatggccgtc
      481 agcagagagc tgctggaggt ttgcgatcgc catcacatag cagcctccaa aggaagcgat
      541 gaactctgcc tggcggtcat ttaagatgac cgggcttagg atggaatact ggcggaacag
      601 aaacttattc tgaaaaaaca tagtgattgc ttcttttccg tagagatggt attcgtgctg
      661 ggacgttccg ttggggcagt agtcgcagta atgcccgtct gaagtgaata aatcccccag
      721 tgctttaaaa tccttcgttt caagtgcttc tatatatcgt tcaatggcag aatgttccgt
      781 catggtgtct gatccttctt tcttttatat ctgaatttct aataaacctt ttccgtattc
      841 aacagcgcat ctgtgccacc gtccacaaac atgacgatcc cgttgacgcc tctggcctca
      901 ggagagatga gaaatgtgat cacagaagcg atttcttccg gatccatctg cttttttgtg
      961 ccgtactttg tggggatcgg aagggcattt aaggccgctg ccgtatcctt tgacccggtc
     1021 gctgtcatag gggtgtttac attcccagga gccacggcat tgatccgaac gccgttggcg
     1081 gcataggagg cagagtggcg gcgcatccag cggcagagtg catacttgga ggcagcgtag
     1141 atctgatctc cagtatttaa gtctgctcca tccatccggc cgatcacctc aaggatccga
     1201 tattcatcgt ctctgttgtt gtttaaaagg tctaccagat cggttttcat atcttccctg
     1261 gaaatggcgc tggaggcaga gatgacacag gcgccgtttt tctgcttcag aagatcatag
     1321 gcgcctttta taatggaaat ggttccgaaa tagttgacgg aaaggatcag acgcaggtct
     1381 tttcgggagc cggggatgcc ggcacagagg atcaggccgt ccagtccctg agggcagagg
     1441 ctgaaaagct cgtcaatggc cttctgcctc ccttcgggaa gagacagatc tgcttcaata
     1501 tctccacgct tccagtctat attgatggtt tcatggcctt tttgctttaa aatggaaatg
     1561 gtttttgctc cgattccgtt agaacctccg gttactgcat agattcccat acgatttcct
     1621 ccctttacaa atggcattac ccctattata atcgaaattg aactctattt aatacccagt
     1681 tttaataaaa aaaaggatac cagataaaaa tgctgcatta tgaattagtg aaaaaaaggg
     1741 ggtgtcccct ctatgaatgt ctttatgaat gtctgaggga ggacattctc agaggccgtc
     1801 tcctgccggg aacaaagctg ccttccagaa gagagcttgc cagagataat gggatcagtg
     1861 tgaaaaccgt ggtgaatgcc tatgaacagc tcctggtgga gggttatctg atcgcaaagg
     1921 agaaaaaggg ctattttgta gcagacgtag agaccatgga tcagtatcat cctgagaaag
     1981 cagaatatcc ccagctgtac cgggaggaga agtggtttgc agattttacg gctaacaata
     2041 ccatatatga aaaattcccc ttttccatgt ggcgaaaggt catgcgggag gtgctttccg
     2101 agtacgatat ggagctggtg cagagagccc attttctggg agtcaggcag ctgagagagg
     2161 ttattgcaga ctatttatac aggctgagag ggattgtggt atctccggaa tgtattgtca
     2221 taggagcagg aattgaatat ctttatggaa agcttgtaaa gctgcttccc ggagactcta
     2281 tttatggggt ggaaaatccg ggatacaaaa agattcccag gatctacgag gaatatggtc
     2341 tatcctggag atatacggaa atggatgaaa atgggatcag tatggaaagc ttaagagaaa
     2401 gcggggcaga tgtgatccat gtgtcaccgg agcatcatta tcctctgggg acagtcatgt
     2461 ctgccgcaag aagacaggag cttttaaatt gggccgtaga aaaggaaggc agatatgtga
     2521 tcgaggatga ttatgactgc gagttccgct accgttcccg ctcaatccct gccctaaaga
     2581 gcatggatcg gaatgggagg gtgatctata tgaatacttt cagcaaaact ctggctcctg
     2641 cgatccggat cagctacatg gtactgccgg aagcgctgat cagccggtat attgataatg
     2701 ccaatttcta ttccaacaca gcctctgcat gtgagcagta tgctctggca aagtttatgg
     2761 agaaagggta ttttgagcgt catctgagcc gccttaagaa atattatcac acagaggggg
     2821 aacggctgat ccggatcatc cgccagtcct ccctgctccc ggtagcagat gtacggggcg
     2881 gagagagcgg aacccatttg ctggtaaagc tggatacgtc tctcacagac ggacagattc
     2941 gtgagaaagc gaagcagcag gggattttag tagcctgtct gtcccagttt tgtaccaggg
     3001 tgcgtccgga gtatgaaagg gtactggtgc tcaattacag cgatatggat gagggacggc
     3061 ttcgtgaagc ggttcgaaga cttggaaatg tatttatgca atggtaaagg atgctaaact
     3121 ggcatccttt tattattttg ttttggctgt tttaaagagc gcagatgggt ggtaggatga
     3181 tggtgtcacg ggggtaacgg atcgcacagg ggttatgaaa caggcggtct gatgaagagc
     3241 ctgggaggca aaaaggcact gatcgttacg gatgcattcc tggagaaaca gggaatggca
     3301 gccatgatgc agggaatttt atccgaagcc ggagtagaga gcgtagtatt tgggggcgca
     3361 gagccaaacc ccaaggatac caatgtagaa gccggattaa gggtatttaa ggaaaacggc
     3421 tgtgattcca ttatctcttt aggcggcggc tcttcccatg actgtgcaaa ggggatcggc
     3481 cttgtagctt ccaacggagg aaatatacgg gattatgaag gaatcgaccg ttccacaaag
     3541 aatatgtgtc ccatggtcgc aatcaataca accgcaggaa cggcgtctga gatcacacgc
     3601 ttctgtatta ttacagatac cagccgccat gtaaagatgg caatcgttga ctggagagtg
     3661 acgcctcaga tcgcaatcaa cgatccgaag cttatggtgg gaatgcctcc gtcactgact
     3721 gctgcaacgg ggatggatgc gttgacacac gctatcgagg cttatgtatc aaccaatgct
     3781 aacccgctga ccgatgcggc ggctctgatg gccattacca tgattaccca gtatctgcca
     3841 aaggcagtag caaacggtac ctatatgaag gccagagata agatggctta cggtcagtat
     3901 ctggcaggta ttgcttttaa caatgcgtca cttggctatg tacacgccat ggcccatcag
     3961 ctgggcggct tttataatct gcctcacggc gtctgcaacg cgatcctgct tcctcatgta
     4021 gaagaattta acctgatcgg aaatatgaac tgtttccgcg atattgccaa ggcaatgggc
     4081 gaaaatattg aagggctttc cgccacagag gctgcaaaga aagcgattca tgcaatccgc
     4141 cagctcagcg agcaggtagg aatcccgaag gatttaagaa gtctgggcgt aaaggaagaa
     4201 gattttgaga tcatggcaga gaatgcaatg aaggatgtat gccagttaac caatccgcgc
     4261 aaggcaacca aggagcaggt aatcggaatt tataaagctg cgtattaaaa aggaggaaat
     4321 cattatgaag aaagcagagc ttcagagtaa atatcagtta ttgatcggag gacagtggaa
     4381 ggacgcttca gacgggaagc tttttaagac cacctgtcct gcagacggga gctttctggc
     4441 agagtgcgcc gaggctacaa gagaggatgt agatgaggcg gtagctgcag cctggaaagc
     4501 atttgaaagc tggaagaata caaccgtggc agagcgggcc gctgtcttaa ataagattgc
     4561 agatatcatt gacgccaata aggagcatct ggctatggta gagtctatgg acaacggaaa
     4621 gcccatccgt gagacgctgg ccgttgacgt gcctatggcg gcagatcatt tccgctactt
     4681 cgcaggctgt atcctggcgg aagagggaag cgccaatatg ctgggaaata cgaccttaag
     4741 cctgatctta agagaaccca tcggcgtggt aggccagatc gtcccatgga acttcccctt
     4801 ccttatggct gcctggaagc tggctcctgt gctggcaagc ggctgctgca gcgtatttaa
     4861 gccatccagc accacttcct taagcgtact ggaatttgca agactggtgc aggatgtaat
     4921 cccggcaggc gtattcaatg taattacagg ctccggctcc cgttccggcc agtatatgct
     4981 ggagcacaag ggctttagaa agctggcatt taccggttct accgaggtag gacgcagcgt
     5041 agctatggca gcggctgaaa aactgattcc atccacgctg gaactgggcg gaaaatctgc
     5101 caatatcttc tttgaggact gtgactggga gatggctatg gacggcctgc agatgggtat
     5161 cctgttcaac cagggccagg tctgctgcgc aggctcccgc gtgttcgtcc aggagagcat
     5221 ttacgaccgc ttcgtagagg aggctgtcaa gcgctttaac caggtgaagg ttgggcttcc
     5281 atgggaagag gatacccaga tgggcagcca gatcgaccgc cgtcagatgg agaagatctt
     5341 aaaatatgtg gaaatcggta aagaagaggg cgcaaagctg ctctgcggcg gtgttcaggc
     5401 caaagagggc gagctggaaa agggctgctt ccttcgtcct accctcttag gtgatgttac
     5461 caaccacatg agagtggctc aggaggaaat tttcggaccg gtggcctgca tcctgaagtt
     5521 taagacagag gaagaggtca ttgccatggc aaatgacagt gagtacggac tgggcggtgc
     5581 tgtatggacc agagatatca atcgtgccct gcgcgtggca agaggcgtgg aaacaggccg
     5641 catgtgggta aatacctata atgcaatccc tgagggagcc ccctttggcg gatataagca
     5701 gtccggcatt ggacgtgaaa cccacaaggt aatcctggat cactataccc agatgaagaa
     5761 tatcatgatc aatctggcag aggttcccac cggattttat cctgcaaaat aaaatataaa
     5821 aaacatcccc tgtaatcagg cggtcagaat gatacattcc ggctggccgg ttacagggga
     5881 tttcttttgg ttacagcgtg atccagtacc gctgcatcac ggttccgtct tctttttcaa
     5941 tttcattttc aaggatgccg ccgttgttca taatgctttt ggcagatcca gtattttcct
     6001 ttttgcaggt cataaggacg cgggtgatac ccagtttttt acattcctca agtcccaggg
     6061 caatcatccg ggtagcaatt cccttccggc gttcagaagg acgtaccccg tctccgatgt
     6121 ggccgccgaa ttttaaaaga tggtcattta aataatgacg gatattgatg gctcccacca
     6181 ggatatttct ctctttatcc aggcagaaaa aggtagaatc gggcaccagg ccgggaaccg
     6241 cctccgtaag ctccaggctg tctaaatagg ctgggaaatc atgaatgtct gcctgatcaa
     6301 tagcacttgg gatgatggct tcaccggagg ccttccattc acggagcata tcctcggcca
     6361 tttcccggta ttcaggcccc atttttacta actgtaaatc cattaacaaa cgcctctctt
     6421 ttgaatcagt cccatcagct cctcatagaa gcggggatag gagatattga cacagtccgc
     6481 gccctgtatg gttgtgagtc cttccgcaca ctgggcggtc acagcaaagg tcatggcgat
     6541 ccggtgatct aagtggctgt tgatcacagc tccatggagg ggctttccgc cgcggatgat
     6601 catgccgtcc tctgtttcag tcacatcagc gcccatggca gtgaggtttt caaccatgac
     6661 ggcaatgcgg ttggattcct tgacttttaa ttcccctgca tcacagatcc ttgtttcccc
     6721 ttctgccgca gccgccatgg cagcgatcat gggaagctcg tcaatgaggg taggaatgat
     6781 ggcaccgccg acggtggttc cgtgaagggc gctgtggcga accaggatat ctgccgttgg
     6841 ttctccctcc tggccccgta catttaaaag ggtcagatcg gctcccatgg cgcggcatac
     6901 atggagaagc ccgtctctgg taggattgat gcctacattt ttgatcagga tttcagagcc
     6961 gggaaccatc agaccggctg cgataaagta ggcggcggag gaaatatctc ccggcaccat
     7021 gaccttttgt ccgtagagct gggtggcagg ggagatagag gcggttgtcc cttctgtgga
     7081 aacagaggct ccaaatagct ttagcatccg ttcggaatga tttcttgata caaagggctc
     7141 tgtgacctgg gttattccgt cggcataaag gcctgccagc agaatggcgg attttacctg
     7201 ggcagaggcc acctttgagg tgtaatggat ctcatgaagc tttgtaccac ggatcaggag
     7261 cggagcacag tcatttcccc ggatgcttgt gatatcggct cccatccggg aaaggggctc
     7321 cataatccgt ttcatgggcc gtttctggat ggaagcatct cctgtgagag taacgtcaaa
     7381 atcctgtgcg ctgaggatgc ctgagatcag gcgggtggtg gtgccgctgt ttccgcagtc
     7441 caatatagtt tccggttttt ttaggccgtg aaggccgttt ccatgtacca gaaccacatc
     7501 ccctttattt tcaatggaaa cgcccatttt ttcaaaacag gagatggtag aaagacagtc
     7561 ggcgccctga agaaagccgt ggatttccgt ggttcccttt gcaagagagc cgaacataac
     7621 cgcccggtgg gaaatggatt tatcaccggg aactgtgatt tcgccccgga ggggacccca
     7681 ttttttcagt tccataaaat atgattcctt tctgttttac agccgcacca gctcaaactt
     7741 gtatttttca agccgttccc aggcctcctt catggaagcc tcgtcataaa aggcaatttt
     7801 taaagcgcct tcgccgtgtt ctctgttgtt gttgatgccg atatttttaa tactgatgcc
     7861 ctttgcggct aaaataacgg caatagtaga aatggatccc acctcgtcca ccacatcaac
     7921 tgaaaattca taggagggtt cgatagggcc ttttgcccgg tctgtaatgg aatctcggta
     7981 ggtgcgtgat ttttcaaaca gccggtaaat ctctttgtcc ttgtgtccgc ggatctgatc
     8041 cagaatatcc tgaagggagc cgatatactg ctccagcata tctccgatgg gcccggcgtt
     8101 tgtcatgcag atctgctccc acatttcagg agagcaggag gcgatgcggg taatgtcctt
     8161 aaagccgcct gctgcgaccc tgcgcatgat gccttccggg gaatcgctgt cctttaccag
     8221 gtttaccagg ctggaggcca ccagatgggg cagatggctg atggcagccg ttacccggtc
     8281 atgctccctg taatccagca ccagagggat tgctcccaca gcctgagcca ctgccagcag
     8341 acggcagacg ttgtccgaat aggatgcaga gggctctgtc aggccttctg gcggggtcac
     8401 aatataatag gcattttcca aaagatgatc tgtagaatgt tcatagcctg ttttttcgga
     8461 tccggccatg ggatggcctc ccacaaaaca gtgggaaagg ccctggcgca ggatttcttt
     8521 atggatatct gttttggtgc tccctacatc tgtgatcaat gctcccggtt ttaaataggg
     8581 gcggatggca gagagatact gggcattata ctccacagga gtacatagga aaatgatatc
     8641 acattccagg agctcttccc cgatcccatt taagatcacg tctataatac cgtctttttt
     8701 ggcctgctct aatttggagc gggtccgcat ataggccata atgcggatat ctggacgggc
     8761 ccgcttgagc ccccttgcaa gggaaccgcc gatcagtccc agaccgataa aggctatagt
     8821 ggagtccttg attgtaaatt gttccataag atgcagcccc ctttatctta agatttccct
     8881 tttaccgtgt gaccgttctg cctgccagat cacagtatgg ctttaagtcg gccatgagct
     8941 tttcaaaggc gtcaaaggtc agagactggg ggccgtcaga cagggcgcag gccggatttg
     9001 ggtgaacctc gatcatcaat ccgtctgctc ctacggcgat cgcagccttt gccagaggtt
     9061 ctacataggc tcttacaccg gtggcatggc tggggtctac gataataggg agatggctct
     9121 tctggcggat cacaggcacg gcgctgatgt ccagagtgtt gcgggtagag gtctcatagg
     9181 tgcggatacc gcgctcgcag agtacgatat ttggattccc ctcagaaatg atgtactctg
     9241 ctgcattgag ccattcatca atggtagcgc acaggcctct ctttaagaga acgggcatac
     9301 cggattttcc tgcctccttt aaaagctcaa aattctgcat atttctggcg ccgatctgga
     9361 gcatatccac atatttggag gcagcctcga tggcgtgggc gctggtgacc tcgcagatca
     9421 catttaaacc ggtggcttct ctggcttcct tcatatattt taagccctct tcctctaagc
     9481 cctggaagga gtagggagaa gtacggggct tgtaagcgcc gccccggagg aaggtagctc
     9541 cggctttctt tactgcaaag gcggtttcca ttaactgttc tcttgactca atggcacagg
     9601 ggccggccat aacggtgagc gtcccagggc cgatatgggt attgcctacc gggatcacag
     9661 aatcatccgg atggaatttc ttatttacaa gcttatagga ttcagtgacg gaaacaacct
     9721 tgtctacgcc ttcgctcatt tcgatgttgc ttcccatcag ctttgtctta tcgcctacca
     9781 cgcctacgat ggttacctgg gagccctcgg aaagatgggg ctgaagtcct tttgcttcaa
     9841 tcagatcggt tacttttttt acagcctctt tggaggcgtc tggcttcatg ataatgatca
     9901 tttgtattct ccttttcgta gggacactgc tgtctgccct aacctctttt tctgatatcc
     9961 ttcagtttaa agcctgtgtc tgaaaatgtc aacactttat tactttaaag tttcacactt
    10021 ttatgtacaa aatcatcatg cggcgcaaag gtgtgtaagc aaaaaatgtc aaagaattgg
    10081 aaaagccggt ttagttttat gatctttcac ggacattaaa ataaaaataa aaatttcatt
    10141 gtgcaaataa gtcaaaaagc ttgaaaaatt ctgaaaaatt tagtagaata gacacatggc
    10201 ttaaataaat atgcacagga gggaagttta tgaacgtata tggtactgaa caaatccgca
    10261 acgttgtatt acttggacac ggaggcgccg gtaagacgac cgttgcggag gctctggcat
    10321 ttattacagg tgtaacaaag cgtatgggta aggtgcccga cggcaacacc atcagtgatt
    10381 atgacaaaga agagatcaag aggcagttct ccatttccac caccctcatt cctctggaat
    10441 atgaaggaga aaacgggcct gttaagatca atctcctgga tactcccgga ttttttgatt
    10501 tcgtgggaga agcagaggaa gctatcagcg tagcagacgc tgccattatc gttgtaaact
    10561 gcaaggccgg cattgaaccc ggcacagaga gagcatggga gatgtgcgaa aagtacaatc
    10621 tgcccagact gattttcgtc accaatatgg atgatgatca tgccagctat cgtgagctgg
    10681 tggtaaagct ggaaaagaag ttcggacgta agatcgctcc gttccagctg cctattcgtg
    10741 aaaatgaaaa attcgtgggc tttgtcaacg tggttaagat ggcaggacgc cgttttacta
    10801 ctttaagcga ttatgaggag tgcgagattc ccgactatgt tcagaagaac ctgaccatcg
    10861 caagggacgc tctcatcgag gccgttgcag agaccagtga ggaatatatg gaacgctact
    10921 tcctgggcga agagtttacg caggaagaaa tttcacaggc actcagaact catgtaatcg
    10981 agggcgatat cgttcctgtt atgatgggtt ccggcattaa ctgccaggga tttaaggtgc
    11041 ttttgcaggc catggacaaa tacttcccgt caccggatca ttttgagtgt attggcgtag
    11101 acgtatccac aggcgaacgc tttactgcaa agtacaatga tgatgtatcc ctgtctgcaa
    11161 gagtatttaa gaccattgta gatccattta ttggaaaata ttctctgatg aagatctgta
    11221 ccggtacgtt aaagggagac acaacggtat ataatgtaaa taaggatgct gaggagaaaa
    11281 tcggaaagct ttatgtgctc cgcggtaagg acgtgatcga ggtttccgag ttaaaggccg
    11341 gtgatatcgg agcagtggca aagcttacag tgactcagac cggagacacc atggcagtgc
    11401 gcaccgcacc gatcgtatac cacaagcctc agatttccac tccatataca tatatggcct
    11461 acaaggcagt aaataagggc gaggatgaca aggtatccac cgctctggca cgtatgatgg
    11521 aagaggatct gaccctgatc gttaaaaatg acgcagaaaa ccgccagtct ctgatctatg
    11581 gaatcggcga ccagcagctg gatgtagtgg taagcaagct gcagtctcgt tataaggtgg
    11641 cagttgagct ttccaagccg aaatttgcct tccgcgagac cttgcgaaag aaggtaaagg
    11701 tacagggcaa gcacaaaaag cagtccggcg gccatggaca gtacggcgat gttatcatgg
    11761 agtttgagcc ctccggcgat ctggagacac cgtatgtatt tgaggagaag gtattcggcg
    11821 gttccgtacc gaagaattac ttccccgcag ttgagaaggg gcttcaggag tgcgtacaga
    11881 aggggcctct ggccggttat ccggtagtcg gcttaaaggc gactctgtta gacggttcct
    11941 accatccggt agattcctct gagatggcat ttaagatggc tactattctg gcttttaagc
    12001 agggattcgt
//
LOCUS       DBMDUH010000076        11729 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_2775_length_11729_cov_8.289904, whole genome shotgun sequence.
ACCESSION   DBMDUH010000076 DBMDUH010000000
VERSION     DBMDUH010000076.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 11729)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11729)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11729
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2775_length_11729_cov_8.289904"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="ACZPCG_07715"
     CDS             <1..882
                     /locus_tag="ACZPCG_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007719727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HIR1284989.1"
                     /translation="ILRPGRFDRKVAVGRPDVKGREEILKVHSKEKPLGEDVDLHRVA
                     QTTSGFTGADLENLMNEAAILSARENRRFIRQNDIDRAFVKVGIGAEKRSKVISEKDK
                     KITAYHEAGHAILFHVLPDVGPVHTVSIIPTGMGAAGYTMPLPEKDEMFNTRGRMLQS
                     IMVDLGGRIAEELIFKDVTTGASQDIKQATQLARAMVTQYGMSERVGMIQYGSDEDEV
                     FIGRDLAHTKSYGNGIADVIDEEVKRIVDECYAKAKQIICDHEDVLHSCAALLIEKEK
                     IGQDEFEGLFAETEVKA"
     gene            1370..3412
                     /locus_tag="ACZPCG_07720"
     CDS             1370..3412
                     /locus_tag="ACZPCG_07720"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006778329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HIR1284990.1"
                     /translation="MEPKEGERVRLRLRTAKADADQVYYIEGEKEAVMKKTASDPYFD
                     YYEHEIVVASDQVLYHFKVKKNKEICQYNRLGPVDEADPEFDFKITPGFYTPDWAKGA
                     VMYQIFTDRFCNGDPDNDVESMEYVYIGKPVYRVKDWGRNPSTMDVGCFYGGDLKGVG
                     DKLDYIQSLGVEAIYLNPVFVSPSNHKYDCQDYEHIDPHFGRIVKDGGKLVDKNGTDN
                     RQAERYVTRAATRENLEASDALFAELVEEIHRRGMKVILDGVFNHCGSFNKWLDAEEI
                     YERSGDYEPGAYLTKDSPYHSFFQFHDDSDEAWPRNRTYDGWWGHDTLPKLNYEGSEK
                     LVKYILNVAKKWISPPYSVDGWRLDVAADLGHTAEYNHTFWQMFRKAVKEVNPEVLIL
                     AEHYGDPASWLQGDQWDSIMNYDAFMEPVTWFLTGMEKHSDGYNGALYGDGEAFFGAM
                     NYHMSRMQTNTIFTAMNQLSNHDHSRFLTRTNQIVGRLGNMGPERASEHVKKPVLREA
                     VVIQMTWPGAPTLYYGDEAGVCGWTDPDSRRTYPWGKEDLELIEFHRYMTGIHKRIPA
                     LRKGALKPLFAGRHQIAYGRMSGDHQTITVISNRPEPAVMEIPVWQLGITDDMILGRP
                     ILTFEDGYNAGIVFYRVENGMLKVIMPPYGGAVFVSRPEAFYPVIESRFVGGEEVL"
     assembly_gap    3782..3881
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3955..4344
                     /locus_tag="ACZPCG_07725"
     CDS             3955..4344
                     /locus_tag="ACZPCG_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1284991.1"
                     /translation="MDLVGSCGTGHAVIRSITFDVEEALMEAEVVLVSAMAKRHEAIT
                     DWIAPHLKDGQIVCFSAGNCASITLKKKRGDKKVIVGEMQGNIGSCRLVSPTKEIKGN
                     PHTLLPPSYDWDSEYLRITGTPRKVSE"
     gene            complement(4463..4548)
                     /locus_tag="ACZPCG_07730"
     tRNA            complement(4463..4548)
                     /locus_tag="ACZPCG_07730"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4512..4514),aa:Leu,seq:taa)
     gene            complement(4641..4722)
                     /locus_tag="ACZPCG_07735"
     tRNA            complement(4641..4722)
                     /locus_tag="ACZPCG_07735"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4686..4688),aa:Tyr,seq:gta)
     gene            complement(4796..5650)
                     /locus_tag="ACZPCG_07740"
     CDS             complement(4796..5650)
                     /locus_tag="ACZPCG_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIR1284992.1"
                     /translation="MKKSDFMKEFIVITFATLIVSAAVFFFLIPSQVSVGSISGLAMI
                     LGNIIPLHISIITFILNGLLLIIGFLLIGKEFGAKTVYTSLLLPLFLRIFEIWFPDFS
                     SINGDPFLDMVCYIFVVSIGLAILFRENASSGGLDIVAKLLNKYFRMDMGKALALPGM
                     CVALSSILFYDKKLVVLSLLGTYLNGLVLDHFIFGLNLKKRVCIISEKEAEIRSFILN
                     RLHSGATIYEGRGAYDGQPKTEIITIVDKNEYALLMSYLLKTDPSAFVTVYTVSEVIY
                     RPKPRQIL"
     gene            5884..7932
                     /locus_tag="ACZPCG_07745"
     CDS             5884..7932
                     /locus_tag="ACZPCG_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HIR1284993.1"
                     /translation="MKDKGKMRWTFRVYLQWPLYLTVFMVLLAVAVGAVSLTAGIIVS
                     AFTLVYIGIALWLYCSRKRGILAGLIAFAEAYDQSKQDILKEMLVPYAVTDRTGRLLW
                     MNREFSLILGEDKTAAPNLTALFPEVTKEMLATGGETVSIHSSYLERNYRVDLRELAI
                     ASLSQAVADNGLETGEGGITAVYLMDETQTLKYKQQVNDQKLVAGLIYLDNYEEALES
                     VEEVRRSLLTALIDRKISKYIASMNGIVKKIEKDKYFFAICQQHMAKMQESRFPILED
                     VKTVNIGNEMAVTLSIGIGMNGESYAQNYEYARTSIDMALGRGGDQAVVKDSDKIQYY
                     GGKAQQMEKTTRVKARVKAHALRELMENKDRLLIMGHRLADIDAFGAAIGIYRIAMSM
                     NKKANIVLNEVTSSVRPMMERFLDNAEYPDDMFLKGPQAAELADAGTMLVVVDVNRPS
                     ITDEPSLLRLVRTIVVLDHHRTSNEVIDNAVLSYVEPYASSTCEMVAEVLQYIADGIK
                     VRPAEADAMYAGIVIDTQNFTNQTGVRTFEAAAFLRRSGADITRVRKLFRENMKDYQA
                     KAEAVRAAEVYMDAFAISVCPAEGLDSPTITGAQAANELLEIKGIKASVVLTDYNNTI
                     YFSARSIDEVNVQVMMEKLGGGGHRTIAGAQMQGVSTEEAKDRLKEVIRQMLEEGDVS
                     "
     gene            7954..8400
                     /gene="rplI"
                     /locus_tag="ACZPCG_07750"
     CDS             7954..8400
                     /gene="rplI"
                     /locus_tag="ACZPCG_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HIR1284994.1"
                     /translation="MEIVLLEDVKALGKKGQVVKVNDGYARNFILPKKLGVEATAKNL
                     NDLKLQKANDAKVAAEQLAAAKELGEKIEKSKVVVSIKAGEGGRAFGAVSSKEIAKAA
                     ADQLGLDIDKKKIVLNDPIKAIGSFEVAVKLHKEVTAKLAVKVVEA"
     gene            8529..9887
                     /gene="dnaB"
                     /locus_tag="ACZPCG_07755"
     CDS             8529..9887
                     /gene="dnaB"
                     /locus_tag="ACZPCG_07755"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HIR1284995.1"
                     /translation="MEEALIKRVLPHSLEAEQSVIGSMLMDREAVIAASEIITGSDFY
                     QQQYGIMFDAMVELFNEGKPVDLVTLQDRLKEKDVPPEVSSLDFVRDIMTMVPTSANI
                     KSYANIVSEKAVLRRLIKTTEEIAGACYAGKEPLENILADTEKSVFDLLQNKGGQEFV
                     PIKQVAINVLEKIEDAYRNQGTVTGIPSGFIDLDYKLSGFQPSDFILIAARPSMGKTA
                     FVLNVVDYVSVRKNLPCMVFSLEMSKEQLVNRMLSMESNVDSQKLRTGTLTDADWDAV
                     VEGIGVIGNSKLIIDDTPGISITELRSKCRKMKLEHGLSLVIIDYLQLMSGSKKGGND
                     NRQQEISEISRSLKAIARELHAPVIALSQLSRACETRTDHRPMLSDLRESGAIEQDAD
                     VVMFLYRDDYYNKDTEHPNEAEVIIAKQRNGPIGTVNLIWKPEYTKFVNAARPQGGNS
                     EG"
     gene            10002..10700
                     /locus_tag="ACZPCG_07760"
     CDS             10002..10700
                     /locus_tag="ACZPCG_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006782742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR1284996.1"
                     /translation="MDEVHYLISDASRKVDVEAHVLRYWEEELELDIPRNEMGHRYYT
                     DFHIRMFRQVKSLKEKGYQLKAIKHALNQVLHQDGENGAQLTDELLEKDMDAALRGFK
                     EEERLAPLRPVQAETIPAVSMEERMAQFQQIMNLIIGRALEVNNEKLSQDISSLVNEK
                     LSGEMELLLQSSDRKEEERFRQLDEVIRSFQKSGQAEAAAARVPFFKRKPFKRSGKSR
                     NASGHPQRSGGLIP"
     gene            10849..>11729
                     /locus_tag="ACZPCG_07765"
     CDS             10849..>11729
                     /locus_tag="ACZPCG_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006772029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HIR1284997.1"
                     /translation="MNQRFRYRKYLTYALASGILAASHTLGGCADQTAGGMAATPAQA
                     QARAAAPEEETEAIAPLNLSGQILPDLDDVELPENAEPVPEYLREGVRHEIVKKLQQR
                     LMDLGFMDNDEPTDYFGQVTLQAVKHFQRQNEMPQDGIVGDATWEALMAEDAKYYAVS
                     KGTQGDDIQRIQQRLYELGYLAKADQVTGNFGDSTEAAVIKLQEVNGLSPDGKVGQKT
                     YNLLYSDEVRPNLLSYGEKSEVVLACQQRLKELGYLTTTPDGAYGQDTVIAVKQFQAR
                     NDQVVDGYLGPSTRIAL"
BASE COUNT         3245 a         2397 c         3258 g         2729 t
ORIGIN      
        1 atcctgcgtc ccggccggtt tgaccgcaag gttgctgtgg gacggccgga tgtaaaggga
       61 agggaagaga tcttaaaggt tcattccaag gaaaagcccc tgggagagga cgtggatctg
      121 caccgggtag ctcagacgac ctccggcttt acaggtgcgg atctggaaaa cctgatgaat
      181 gaggcagcaa tcctttctgc aagagaaaac cgccgtttta tccgtcaaaa tgatattgac
      241 cgggcttttg taaaggtggg gatcggagct gaaaaaagga gcaaggttat ttccgaaaag
      301 gataaaaaga tcacagctta ccatgaggca ggccacgcga tcctgttcca tgtgcttccg
      361 gatgtggggc cggttcatac ggtttccatt atccctaccg gtatgggggc tgcaggctac
      421 accatgcctc ttccggaaaa ggatgagatg ttcaacacca gaggccgtat gcttcagagc
      481 atcatggtgg atctgggcgg acgcattgca gaggagctga tctttaagga tgtgacaacc
      541 ggcgcttctc aggatatcaa gcaggccact cagctggcaa gagctatggt gacccagtat
      601 ggtatgtccg aacgtgtggg aatgatccag tacggcagtg atgaggatga ggtatttatc
      661 ggccgtgatc tggcgcatac caagtcttat ggaaatggaa ttgcagatgt cattgacgag
      721 gaagtaaaga ggattgtaga cgaatgctat gcaaaggcaa agcagatcat ctgcgaccat
      781 gaggatgtgc ttcattcctg tgcggctctt ctgatcgaga aggaaaagat cggacaggat
      841 gagtttgagg gtctttttgc tgagactgaa gtaaaggcat aaaggctaga tttcatttgt
      901 ttgagaaaaa acagaaacgt tttgagcata aaaacggagc ttgattcgta tttgcgaaat
      961 tcaatcgtta ttttgactgt aaagggcgga gaaaaaaatg catgtttgtg cacacttttt
     1021 tctccgcctg tgctattata atagacgtaa cccccccaat acattatata gtttttgcta
     1081 caccccataa gaaacgaaat accttctcct gaaaagcctg gctaccccgc caggcttttc
     1141 actttataaa aacaatgagg aaaatccggg ctttgtgcgc gtgggacatt caccggattg
     1201 cgggattttc cttgtatatt taagtaaatt cagatacaat agagatgcta attcaaaata
     1261 gtatgaatca ttatgaaaag gtaaggaatt cattgtgaaa gaagagggaa caaataagat
     1321 caatcgggag gcgctgtttt cagcagaaac agaggattac cgcagcccta tggagccgaa
     1381 agaaggggaa cgggtacgtc tgcggctgcg taccgcaaaa gcggacgcgg atcaggtata
     1441 ttatatagaa ggggagaagg aggctgttat gaagaagaca gcttctgatc catattttga
     1501 ttattatgag cacgagatcg tagtagcttc cgatcaagtg ctttatcatt ttaaagtaaa
     1561 aaagaataag gagatctgcc agtataaccg tttgggtccg gtggatgagg cagatccgga
     1621 atttgatttc aaaatcacac cgggatttta tacgcctgac tgggctaagg gagcagtgat
     1681 gtaccagatt tttacagacc gcttctgcaa tggagatccg gacaatgatg tggaatctat
     1741 ggaatatgta tatatcggaa agcctgtata ccgggtaaaa gactggggga gaaatccgtc
     1801 taccatggat gtaggctgct tttacggcgg agatttaaaa ggtgtcgggg acaagctgga
     1861 ttatatacag agcctgggag tggaggctat ttatctgaat cctgtttttg tatcaccgtc
     1921 taaccataag tatgactgcc aggattatga gcacattgat cctcattttg gccggattgt
     1981 caaagacgga ggaaagctgg tggataaaaa tggaacggac aaccgacagg cggagcggta
     2041 tgtgacccgt gcggcaacca gagaaaatct ggaagccagc gacgccctgt ttgcggagct
     2101 ggttgaggag atccacagac ggggaatgaa ggtgattctg gatggtgtgt ttaaccactg
     2161 cggttccttt aataaatggc tggatgcgga ggagatttac gagcgttccg gagattatga
     2221 gccgggagca tatcttacaa aggacagccc gtaccacagc tttttccagt ttcacgacga
     2281 cagtgatgaa gcatggccaa gaaacaggac gtacgacggc tggtggggtc atgatacgct
     2341 gcccaagctt aattatgagg gttctgaaaa gctggtaaaa tacattttaa atgtggcaaa
     2401 aaaatggatt tccccgcctt acagcgtaga cggctggcgt ctggatgtgg cggctgacct
     2461 ggggcatacg gcagagtata accatacgtt ctggcagatg tttcgaaagg ctgtaaagga
     2521 agtaaatccg gaggttctga tcctggcgga gcactatggc gatcccgcca gctggcttca
     2581 gggggatcag tgggattcca ttatgaatta tgatgcgttt atggagcccg tgacctggtt
     2641 tttgacaggg atggagaagc acagcgacgg ttataacggc gctctttacg gcgatggaga
     2701 ggcgtttttc ggagcaatga attaccacat gagccggatg cagacaaata cgattttcac
     2761 ggccatgaac cagctttcca accacgacca ttcccgcttt cttacccgga cgaaccagat
     2821 tgtaggacgt ttggggaata tggggccgga gcgtgcctcg gagcatgtga aaaagccggt
     2881 tctcagggag gctgtagtca tccagatgac ctggcccgga gctccgactc tgtattacgg
     2941 tgatgaagcg ggcgtctgcg gatggacaga ccctgacagc agaagaacct atccctgggg
     3001 gaaggaggat ctggaactga ttgaatttca ccggtatatg accggaatcc acaagcggat
     3061 tccggcgctg cgcaaaggcg cgctgaaacc tctttttgca ggacggcatc agatcgccta
     3121 cggaagaatg agcggggatc accagaccat taccgtgata tccaaccgcc ccgaaccggc
     3181 tgtgatggag atccctgtat ggcagctggg aattacggac gatatgatcc ttggcaggcc
     3241 gatcttgacc tttgaggatg ggtataacgc aggaatcgtg ttttaccgtg tggaaaatgg
     3301 gatgttgaaa gttatcatgc cgccatatgg tggcgctgtc ttcgtgtccc ggccagaggc
     3361 gttttatccg gtgatcgagt cgcggtttgt gggtggggag gaagtgcttt aaagctggat
     3421 caggttgtat ctgaggtaaa ggaatcagaa gaatgggagg gcgtaaaaat gagcatttta
     3481 tcggtcggaa ttgagaaggg aaatgcagaa ggtatgatat aggcccaatt tcggaaactt
     3541 tggaaaagcg aatccgcagc gaaacggatc tggagacgct gggggtatgg cttaaaaagg
     3601 ctgccagggc ctctacagta gaggagtttg agcaggaaat tgcataaaat atttcaaagg
     3661 gtattgcaaa cggaaacatt gagtttgcag tacccttttt ttaaaaataa tcgttcgtga
     3721 tggaagaaaa acaatattca tatcaaaaaa ttatccttct aatgcccata tctggataaa
     3781 annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntttccctga tttttgagag
     3901 taaatatgtt ggcacataac ttgcttaaag gccattcagg cagatgggaa tggtgtggat
     3961 ttggttggca gctgcggaac cggccatgcg gtgatccgca gcattacgtt tgatgtagag
     4021 gaagccctga tggaggctga ggtagtgctt gtctctgcta tggcaaaacg ccacgaggcc
     4081 attacggact ggattgcacc tcatctgaag gatgggcaga tcgtatgctt ctcagcagga
     4141 aactgtgctt ccattactct gaaaaagaag cggggagaca aaaaggtgat tgtgggcgag
     4201 atgcagggaa atatcggctc gtgccgtctg gtatccccta caaaggaaat aaagggaaat
     4261 ccccataccc ttcttcctcc gtcttatgac tgggattcgg aatatctgcg tatcacagga
     4321 acgcccagaa aggtttctga ataaacggat ataaaaagaa aaagcgctga aggaacgaat
     4381 ctgcgttccg gcagcacttt tgtttctgct aaatatacag caaaaaacct ctgtttcaaa
     4441 agaaacagag gttaaaatca aatgccgcag accggaatcg aaccggtacg ggtatcacta
     4501 cccacgggat tttaagtccc gggcgtctgc cagttccgcc actgcggcat tccatatagg
     4561 agtatatgca tacttggcct gaaaacaaga gcctttacat gcaaagcact gatgctctca
     4621 agcttactct ggtatgcaac tgggcgaagg tggattcgaa ccaccgaagg cagtgccagc
     4681 agatttacag tctgtcccct ttggccactc gggaattcgc ccatgctttg tttgataagc
     4741 agtgataata atatcacatg agcggggaaa aatcaagagg gggaatgaaa ttattttata
     4801 aaatttggcg gggtttcggc cggtagatta cttcgctgac tgtgtaaact gtgacaaagg
     4861 cggatgggtc tgtttttaaa agatacgaca tcaaaagggc gtattcattt ttatccacga
     4921 tggtaatgat ctcggttttg ggctggccgt cataggcgcc ccggccttca tagatggtag
     4981 cgccgctgtg aaggcggttc aggataaaac tgcgtatttc agcttctttt tcagagatga
     5041 tgcagactcg ttttttcaga ttcaggccga atatgaagtg gtccagtaca agcccgttta
     5101 aataggtgcc taaaaggctt aaaacgacca gctttttatc ataaaacagg atagaggaca
     5161 gggccacgca cataccggga agcgccaagg cttttcccat gtccatgcgg aaatatttgt
     5221 ttaacagctt tgccacaata tccagtcccc cggaggaggc attttcccgg aataaaattg
     5281 caagtccgat gcttacaaca aagatatagc agaccatatc cagaaaggga tcgccgttaa
     5341 tggatgaaaa atcggggaac catatttcaa atatccttaa aaataatgga agcagaagag
     5401 aggtgtaaac cgtttttgcg ccaaattcct ttccgatcag gagaaagccg attatcagca
     5461 aaagcccgtt aaggataaaa gtaatgatgg agatatggag agggatgata ttgccaagga
     5521 tcatagctag accggatatg cttcctacag acacctgact ggggatcaga aagaagaaca
     5581 cggcggctga aacaatgagg gtggcaaagg ttattactat aaattctttc ataaagtctg
     5641 attttttcat atgaatactc ctgtattaaa gatattctct gctgtcattc ctactatatt
     5701 cgattctttt ttacgtgtaa accgtttttt gtgcgtatct tttattttgc cttacggttt
     5761 tgaaaaggct tatgaaagct cctgcgtttc aaaatccgtt ttccaatgga cgaatccggg
     5821 cagatatggt atactctata agataggcgt attccatttt cgggtacaaa ttggaggaag
     5881 gatatgaaag ataaaggaaa gatgcggtgg acatttcgcg tatatctgca gtggccgctg
     5941 tatttgacag tctttatggt cctgcttgcg gtagctgtgg gggcggtgtc gcttacggcg
     6001 ggaatcattg tttcggcttt tactcttgtc tatattggaa ttgcgctgtg gctttactgt
     6061 tccagaaaaa ggggaatcct ggcaggactg atcgcatttg cggaagctta cgatcagagc
     6121 aagcaggata tcctgaaaga aatgctggtt ccctatgcag tgacagaccg gacggggcgt
     6181 cttttgtgga tgaaccgcga gttttcattg atcttagggg aagataagac ggcagcgcct
     6241 aatctgacgg ccctgtttcc ggaggttacg aaggaaatgc tggctacagg cggagagaca
     6301 gtgagcatac acagctcata tttggagaga aattaccggg tagacctgcg ggagctggcg
     6361 attgcttcct tgtctcaggc cgtggctgat aatggtctgg aaacgggaga aggcggcatt
     6421 acggctgtct atctcatgga tgagacccag acgttaaaat acaaacagca ggtcaatgat
     6481 cagaaactgg tagcaggact gatctatctg gataattacg aagaagcgct tgaaagcgtg
     6541 gaagaagtgc ggcgatccct gcttacggcc ctgatcgacc gtaagatcag caagtatatt
     6601 gcttccatga acggcattgt aaaaaagatt gaaaaggata aatatttctt tgcaatctgc
     6661 cagcagcaca tggctaagat gcaggagtcc cgtttcccca ttctggaaga tgtaaagact
     6721 gttaatatcg gaaatgagat ggccgtgacc ttaagtatcg gtatcggcat gaacggtgaa
     6781 agttatgctc agaattatga gtatgccaga acctcgattg atatggctct gggacggggc
     6841 ggcgaccagg cggtcgtaaa ggattcggat aagatccagt attatggcgg caaggcacag
     6901 cagatggaaa agacaacccg cgtgaaggcc cgtgtaaagg cccacgccct gcgggaattg
     6961 atggaaaata aggatcggct gctgatcatg ggccaccgtc tggcagatat tgacgccttc
     7021 ggcgcagcca tcggcattta ccggatcgcc atgtccatga ataaaaaggc caatattgtg
     7081 ctcaatgagg ttacttcctc cgtccgtccc atgatggagc gcttcctgga caatgcggaa
     7141 tatccggacg atatgttcct aaaagggcct caggcggcag agctggcaga tgcaggcacc
     7201 atgcttgtgg tggtggatgt aaaccgtccc agcatcacag atgagccgtc ccttctgcgc
     7261 ctggtgcgga cgatcgttgt gctggaccac cacagaacca gcaacgaggt tattgacaat
     7321 gcggtgcttt cctatgtaga accctatgct tcttctacct gtgaaatggt ggcagaggtg
     7381 ctgcagtata ttgcagacgg catcaaggtg cggcctgcag aggcagacgc catgtatgcg
     7441 ggtattgtga ttgatactca gaattttaca aatcagaccg gcgtgcgtac ctttgaagca
     7501 gcggccttcc tgcgaagaag cggggcggac attacgagag tgcgcaagct gttcagggaa
     7561 aatatgaagg actatcaggc caaggcagag gctgtgcggg cggcagaggt gtatatggat
     7621 gcgtttgcca tcagcgtgtg cccggcagag gggcttgaca gtccgacgat caccggagcc
     7681 caggcggcta atgagctcct tgaaatcaag ggaataaaag cttccgtggt tctcaccgat
     7741 tacaataata ctatttattt cagcgcccgt tccattgacg aggtcaatgt acaggtaatg
     7801 atggagaagc tgggcggcgg cggccaccgt accatcgccg gagcccagat gcagggcgtt
     7861 tccacagaag aggcaaaaga ccgcctgaaa gaagtaatcc ggcagatgct ggaagagggc
     7921 gacgtatcat agagacagaa aggatggatc aatatggaaa tcgtattatt agaggatgta
     7981 aaggcacttg gaaaaaaagg tcaggttgta aaggtaaatg acggctatgc gagaaacttt
     8041 atcctgccta aaaagctggg cgtagaggca actgccaaga atttaaacga cttaaagctt
     8101 cagaaggcaa atgatgcgaa agttgcagca gagcagctgg cagcggctaa ggagctggga
     8161 gagaagattg aaaagtccaa ggtggttgtt tcgatcaagg caggagaagg cggaagagcc
     8221 tttggcgctg tttcttccaa ggaaattgcc aaggcagcag ccgatcagct ggggctggat
     8281 attgataaga agaagatcgt tttaaatgat cccatcaagg ccatcggaag ctttgaggta
     8341 gcagttaagc ttcacaagga agtgactgca aagctggctg tgaaggtagt agaagcgtaa
     8401 aaaggacgta aagggggcgg cgtatcgtat tccggaagga agggatgcgc cgtcctgttt
     8461 tcgcaggctt ccggacagaa aattccggag cgcaggtttg gcaaatatta tagtaggagg
     8521 aaagagtcat ggaagaagcc ctgattaagc gggtgcttcc ccacagtctg gaagcggagc
     8581 agtcggtaat cggctccatg ctgatggacc gggaggctgt gatcgcggcg tcagagatta
     8641 taacaggcag tgatttttat cagcagcagt acggcattat gtttgatgct atggtggagc
     8701 tttttaacga gggaaaaccg gtggatctgg ttactctgca ggaccgtttg aaggaaaagg
     8761 atgtcccacc tgaggtcagc agccttgact ttgtccggga tattatgacc atggtgccta
     8821 cctcggccaa tataaaatcc tatgccaata ttgtaagcga aaaggctgtg ctgcgccggc
     8881 tgatcaagac cacagaggag attgcagggg cctgctatgc gggaaaggaa ccgttggaaa
     8941 atattctggc agatacggag aaatcggtat ttgaccttct tcagaataag ggcgggcagg
     9001 aatttgtacc catcaagcag gttgccatca atgtgctgga aaagatcgag gacgcctacc
     9061 gcaatcaggg aacagttacg ggtattccgt ccggctttat tgacctggac tacaagctgt
     9121 ccggctttca gccctctgat tttatcctga tcgctgcccg tccctccatg ggaaagacgg
     9181 cctttgtgct gaatgtggta gattatgtct cagtgcggaa aaatctgccc tgtatggtat
     9241 tcagcctgga gatgtccaag gaacagctgg taaaccgtat gctgtctatg gaatcgaacg
     9301 tagattccca gaagctgcgt accggtactc ttacggatgc agactgggat gcagtggtag
     9361 agggaatcgg cgtgatcgga aattccaagc tgatcattga cgatacgccg ggcatttcca
     9421 tcacagagct tcgatccaag tgcaggaaaa tgaagctgga gcacggtcta agccttgtga
     9481 tcattgacta cctgcagctg atgagcggca gcaaaaaggg cggaaatgac aaccgccagc
     9541 aggaaatttc cgagatttcc cgttccttaa aggccattgc cagagagctt catgcgcctg
     9601 tgatcgctct ttcccagctg tcccgtgcct gcgagacccg cacagaccac aggcccatgc
     9661 tttcggatct tcgagaatca ggagccattg agcaggatgc ggatgtggta atgtttttat
     9721 accgcgatga ttattacaac aaagatacgg agcatcccaa cgaggctgaa gttattattg
     9781 caaagcagcg taacggccct attggaacgg tgaatctaat ctggaagccg gagtacacga
     9841 agttcgtcaa tgcagcacgg cctcagggcg ggaattcaga gggttaaaat ttcagtcata
     9901 atagcagttc tcaacgcata cattaaaagt aaggtcaaac cggaccgcgc agggcacggc
     9961 ggaggatcgt tacattaagc agtgtgaagg agagaacagt tatggatgaa gtacattatc
    10021 ttatatcaga cgcgtccaga aaggtggatg tggaagccca tgtattaagg tactgggagg
    10081 aagaactgga gctggatatt cccagaaatg agatgggaca cagatattat acagattttc
    10141 atatccgtat gtttaggcag gtaaagagct taaaggaaaa ggggtatcag ctgaaagcca
    10201 ttaagcatgc cctaaaccag gttctgcatc aggatgggga aaatggagcg cagcttacgg
    10261 acgaactgct ggaaaaggat atggatgccg ctttaagagg gtttaaggag gaggaacgtc
    10321 tggcgccatt aaggccggtt caggcagaaa caatcccggc tgtttctatg gaggaacgaa
    10381 tggcccagtt ccagcagatt atgaacctga tcatcggacg ggctctggag gttaataatg
    10441 agaagctcag tcaggatatc agcagtcttg taaacgaaaa gctatccggg gaaatggagc
    10501 ttttgcttca gtcctcagac cggaaagaag aggagcgatt ccgccagctg gacgaggtga
    10561 tccgctcctt ccagaagagc ggacaggctg aggcggcagc tgccagagtg ccgtttttta
    10621 agcggaagcc atttaaaaga agtggaaaaa gcaggaacgc atcaggccat cctcagcggt
    10681 ccggggggct gatcccgtaa aaaaatctcg ccatttatat cggaatatga taatatgata
    10741 ctgaaagtga acgagacagc acaccgggat tttacatccg gtatcatatt gttaaatccg
    10801 gccatatttc aggctgagtc atacaaagga aggatcaaga gcaatttcat gaaccaaaga
    10861 tttagataca gaaaatacct tacatatgcc ctggcctcag ggatactggc agcctctcac
    10921 acactgggag gttgtgcaga ccagacagca ggggggatgg cggcaactcc ggctcaggcc
    10981 caggcacggg cggcagcccc tgaggaggaa acagaagcta tcgcaccttt aaacttaagc
    11041 ggccagatcc tgcccgatct ggatgatgta gagcttccgg agaatgcgga gccggttcct
    11101 gagtacttaa gggaaggcgt ccgccatgag atcgtaaaga agctccagca gcgtctcatg
    11161 gatctgggat ttatggataa tgatgaacct accgattatt tcggccaggt aacacttcag
    11221 gctgtaaaac atttccagag gcagaatgag atgccacagg atggaattgt aggagatgcc
    11281 acctgggagg ctctgatggc tgaggacgcc aagtattatg ccgtttccaa gggaacccag
    11341 ggcgacgata tacagcggat ccagcagcgt ctttatgagc tgggttatct ggcaaaggcg
    11401 gatcaggtaa ctggtaattt tggagattcc acggaagctg cggtaattaa gcttcaggag
    11461 gtcaatggcc taagcccgga tggaaaggta ggacagaaaa cctataacct tctgtacagc
    11521 gatgaggtcc gccccaacct cctgtcttac ggcgaaaaga gtgaggtggt tttagcctgc
    11581 cagcagcgtc tgaaggaatt aggatatctt acaaccacac cggacggtgc ttatggacag
    11641 gatacggtga ttgctgtaaa gcagttccag gccagaaacg atcaggtcgt agacggctac
    11701 ctgggtccat ctacccgtat tgccttaaa
//
LOCUS       DBMDUH010000077        11721 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_2776_length_11721_cov_5.303959, whole genome shotgun sequence.
ACCESSION   DBMDUH010000077 DBMDUH010000000
VERSION     DBMDUH010000077.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 11721)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11721)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11721
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2776_length_11721_cov_5.303959"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(182..1294)
                     /locus_tag="ACZPCG_07770"
     CDS             complement(182..1294)
                     /locus_tag="ACZPCG_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1284998.1"
                     /translation="MASLSLRNVTKKYPNGFVAVKDFNLEIADKEFIIFVGPSGCGKS
                     TTLRMIAGLEDISSGELYIDGKLVNDVEPKDRDIAMVFQNYALYPHMSVYDNMAFGLK
                     LRKTPKDEIDKKVHDAAKILDLEHLLDRKPKALSGGQRQRVAMGRAIVRSPKVFLMDE
                     PLSNLDAKLRGQMRVEISKLHQRLETTIIYVTHDQTEAMTLGTRIVVMKDGVVQQVDS
                     PQNLYDRPCNKFVAGFIGAPQMNMIDAQVAKAGSDITLSFGGHTVALPAAKGKGLEEG
                     GYIGKTVTLGIRPEDLHDEEAFLEANPKSIFQAEIRVYEMLGSEVLLYFDIDDANFTA
                     KVNPRTTARPGDSIKVAMDLDKVHIFDKETEKVIIN"
     gene            complement(1489..2346)
                     /locus_tag="ACZPCG_07775"
     CDS             complement(1489..2346)
                     /locus_tag="ACZPCG_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016289034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIR1284999.1"
                     /translation="MTEKKQQIRDYLAITVGTILIAFAIKNIFDPVSMVTGGVSGAAI
                     ILKELWQVPLWLTNTVLNIPLFLAAFRMMGWQFIGRTLYSTAMLSAALYVLPEMGLVK
                     EDMILSALFGGVLSGVGTGLVFLGGCTTGGTDMLAVLIRGRLRHYSSAQIMQVLDGGI
                     VAAGALVFGIRPSLYAMIAIVCVSRVTDRLIEGMKFSKEAWIISKQDKEIAEAVMERL
                     GRGVTSIKAVGMYSKEEKNLLFCVVSPKEIVKIKSIVREFDPHAFFVVSDAREVFGEG
                     FIEKEDRIT"
     gene            complement(2462..3295)
                     /locus_tag="ACZPCG_07780"
     CDS             complement(2462..3295)
                     /locus_tag="ACZPCG_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvfB family protein"
                     /protein_id="HIR1285000.1"
                     /translation="MIELGKVQELEIVRTKEFGVYLSEKAGDEAAVLLPKKQVPQGAQ
                     IGDKVEVFVYKDSSDRLISTTARPKLQVGECAILEVKDVGKIGAFLDMGLEKDLLLPF
                     KEQSHPVRRGEKCLVCLYVDKSSRLAATMKVYSHMSNQSPYKAEDWVEGTIYEINEQI
                     GAFVAVDNRYYGLIPKIEMYGRFREGDQIKARVMRVREDGKLDLCVRERAYVQMGADA
                     EQVLKVIDEFDGVLPFNDKARPETILREFNMSKNAFKRAVGRLLKEGKIEITEKTIRR
                     I"
     gene            complement(3311..4039)
                     /locus_tag="ACZPCG_07785"
     CDS             complement(3311..4039)
                     /locus_tag="ACZPCG_07785"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016297 - fatty acyl-[ACP] hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HIR1285001.1"
                     /translation="MYQFKSRIRYSETDETGRLSVPGIINYMQDCSTFQSEDGRMGIS
                     YLKEHHRAWLLSGWRIVIDRYPKLGEEITAGTWHCGSKGFYGYRDFVLLDQENNPLVR
                     ANSLWFFYDTEKNMPVRIQPEDILPYGIPQPSQDLGEAPRKILIPEKYETAAAVVIGR
                     HHLDTNHHVNNSQYVEIAKDAIPVGLKIREIRADYKKAARLGDTVIPRITPAGQGQWT
                     VALVNETGELYAAVWLGGQEQPGQ"
     gene            complement(4153..4225)
                     /locus_tag="ACZPCG_07790"
     tRNA            complement(4153..4225)
                     /locus_tag="ACZPCG_07790"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4191..4193),aa:Gln,seq:ctg)
     gene            complement(4262..4334)
                     /locus_tag="ACZPCG_07795"
     tRNA            complement(4262..4334)
                     /locus_tag="ACZPCG_07795"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4300..4302),aa:Gln,seq:ctg)
     gene            complement(4421..4493)
                     /locus_tag="ACZPCG_07800"
     tRNA            complement(4421..4493)
                     /locus_tag="ACZPCG_07800"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4459..4461),aa:Gln,seq:ctg)
     gene            complement(4549..5064)
                     /locus_tag="ACZPCG_07805"
     CDS             complement(4549..5064)
                     /locus_tag="ACZPCG_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003501764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR1285002.1"
                     /translation="MKEEWIQKIAEGDRDTFRDFYKEAAGPVYSFILSLTRDVHEAED
                     LMQDTFLCVWDRAEDYEPQGKPLAWVFTIARHLCYMRFRQRRRETELSEWDKEEEGEV
                     YAALEQAPERKVLSDALAALREEDRQIVLLHAAAGLKHREVASALGLPLATELSKYNR
                     SMKKLQKILQK"
     gene            complement(5105..6292)
                     /locus_tag="ACZPCG_07810"
     CDS             complement(5105..6292)
                     /locus_tag="ACZPCG_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIR1285003.1"
                     /translation="MTEFLVKHFVKGYENTEKDEVRTSYGILASIVGIFCNLLLFGAK
                     LFIGLLVNSVSVMADAFNNLSDAASSIIGFIGVKMAGKPADADHPFGHGRIEYISAFI
                     VAFLVIQVGFSLFKTSVGKILHPELMTFKWISVVILILSICVKFWLSAFNRKLGKRIN
                     SKVMLATAADAMGDVVTTGAAALSLLVFGLWNVNIDGIIGLMVSAAVLFAGYNIAKDT
                     LAPLIGEAIPPEVYEEISSFVEGFDGILGTHDLIVHNYGPSKSMASIHAEVSAGAEIA
                     ESHAIVDRIEREAARKMGLLLVIHMDPVETDNERLNTYRKMLENVLADVDVRLSFHDF
                     RMVGKDKNVCLIFDLVVPREYKDGAVGKLRTRISNEMHRRDPDCRCAITVENSYRSEE
                     GVK"
     gene            complement(6289..7050)
                     /gene="truA"
                     /locus_tag="ACZPCG_07815"
     CDS             complement(6289..7050)
                     /gene="truA"
                     /locus_tag="ACZPCG_07815"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HIR1285004.1"
                     /translation="MNYKMILAYDGTRYNGWQKQGNTDRTIQGKLEQILTRMAGRPIE
                     VFGSGRTDRGVHAEGQTANFHLKQTEEYEDKSLQILEYINTYLPEDIAVLSCEAVPER
                     FHSRLSAARKTYRYQIEMSPKKNVFQRDYYYGLGCRLDQKKMEEAAAFLIGTHDFKSF
                     CGNSRMKKSTVRRIDSIEFEAAGESRLHIRYTGNGFLQQMVRILTGTLIEAGLGKREP
                     EQMKAVLEALNRQAAGFTAPAQGLFLEKVEYGEGK"
     gene            complement(7118..8071)
                     /locus_tag="ACZPCG_07820"
     CDS             complement(7118..8071)
                     /locus_tag="ACZPCG_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004614115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR1285005.1"
                     /translation="MVLGLSACSGSNETNQSQTEAVTKAEPSVVTVKTLNGDKKEVDL
                     EVPFDPKRVAVMDMAALDILEQLGLEERVVGASDTSLDYLEKYMEKEQVAALGTIKEA
                     DMEAVMASEPDVIFIGGRLAKSYDALSGIAPVVYLSIDTQLGTLESVRKNAGIIASIF
                     GLEEQADQLLEGFEERLAALAGFSTDKTAIVGMVTSGGFNVLGNDGRCSLISREAGFE
                     NIGVDAEIDTSTHGNEASFEFVVEKEPDYVFVLDRDAAVGTEGAKLAQEIMENELIMG
                     TEAYRNGNLIYLSNPAVWYTAEGGIRALDQMIGDLEFALLK"
     gene            complement(8108..8860)
                     /locus_tag="ACZPCG_07825"
     CDS             complement(8108..8860)
                     /locus_tag="ACZPCG_07825"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004614116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein"
                     /protein_id="HIR1285006.1"
                     /translation="MRIRELIKKYDGKTVVDSVSFQIPRGKVTSFIGPNGAGKSTVMG
                     MISRLLQRDSGLIEFEGKDIKGWRSRELSRRLAILTQSNQIQMKLTVEELVAFGRFPY
                     SGGRLTDEDKRIIDRAISYMELEAFRERFIDELSGGQRQRAYIAMVIAQDTEYILLDE
                     PTNNLDIYHATNLMKMVRRLCDELGKTVVLVLHEINYAAFYSDYICAFADGKVEKFGK
                     VEEVITKETLSKLYRVEFEIMEIEGKPLSIWY"
     gene            complement(8857..9840)
                     /locus_tag="ACZPCG_07830"
     CDS             complement(8857..9840)
                     /locus_tag="ACZPCG_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009263134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HIR1285007.1"
                     /translation="MQKNAHKTQAGLLLMAVLALGAAALYMLVNVDFENEKLFAYAMR
                     IRAPKLFAMMITAFAIGGASLVFQSVINNTIVTPCLLGMNSLYMLIHTAVIFAAGSGS
                     ILAVNAKAAFGVDLLIMGTAATVIYSTLFRKTGHNVLYVLLIGTVLNSFFGSVQSALT
                     RIMDPNEYDSLLADLVASFANVNSEIMVIAALVLALVVFALRKELALLDVITLGKDQA
                     VNLGVDYDRCIRRLLLGVALFIAVATAMVGPVSFLGLILANLSRQLLKTYRHSQLILG
                     SVFFGIIILVGGQLIVEQVFTYTVPVSVFITFFGGIYFLYLLLTERRRRGL"
     gene            complement(9840..10859)
                     /locus_tag="ACZPCG_07835"
     CDS             complement(9840..10859)
                     /locus_tag="ACZPCG_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004614118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1285008.1"
                     /translation="MILALALLCLSAASLFIGVIDLTPSHLITGGAEQLEILLISRLP
                     RLLAILCTGVGMSVAGLMMQQLCMNKFVSPTTGATISSAQFGILLALLFMPGSTLWGR
                     ALFAFGAALAGTWIFVWFIQRIPFKDAVMVPLVGIMFNNVISGMTSYLAYKYEMTQAL
                     SSWLVGHFSMVLRGRYEIVYLTVPLVILAFLFANHFNIVGMGRDFSENLGVPYHLILF
                     LGLTISAMITASIVVVVGSISYIGLIVPNVISMIKGDRIRGTLTDTALFGAVFVLACD
                     MIGRSVIAPYELPIELIVGIVGSILFIAIIFYRLKYGKKSVVFKLPQIKNMRVMEAGK
                     GRKIQ"
     gene            complement(11058..11435)
                     /locus_tag="ACZPCG_07840"
     CDS             complement(11058..11435)
                     /locus_tag="ACZPCG_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005334015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR1285009.1"
                     /translation="MKLRNILLAVKDIEKSRQFYHKLFGLQTVLNSEGNMILTEGLVL
                     QDEKIWREALGKEIIPENNFCELYFEEPEIEAFICKLETQYPSIQYVNRLTEYSWGQK
                     MVRFYDLDGNLIEVRTPMYKGKL"
     gene            complement(11539..11610)
                     /locus_tag="ACZPCG_07845"
     tRNA            complement(11539..11610)
                     /locus_tag="ACZPCG_07845"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11576..11578),aa:Gly,seq:gcc)
BASE COUNT         3124 a         3157 c         2224 g         3216 t
ORIGIN      
        1 agtgtggttt gaagtatctg atttgaaatc attctatact ctccttttca aacgtattcg
       61 gagtaactaa tatccctatt ttcatatttt aatgcaaaat aacaaaaaaa gcaagagagt
      121 tgtcacctct cctgcctttt tttacaatca gattttttca tttatgaaat ctgctgccct
      181 attagttgat gattaccttc tctgtttcct tatcgaagat atgtacctta tccagatcca
      241 tagcaacctt gatggaatcg ccaggtcttg cagttgtacg aggatttacc tttgcagtaa
      301 agttagcatc atcgatatcg aagtacagaa gaacctcaga acctaacatc tcgtatacac
      361 ggatctcagc ctggaaaatg ctctttggat ttgcctcaag gaatgcttcc tcatcatgca
      421 gatcctctgg acggataccc agagtaacgg tctttccaat gtagccgccc tcttctaagc
      481 ccttgccctt tgcagcagga agcgctactg tgtgaccgcc gaaggataag gtaatgtcag
      541 aaccagcctt tgcaacctga gcatcgatca tgttcatctg aggagcgccg atgaaacctg
      601 caacgaactt gttgcatggt ctgtcataca gattctgagg gctgtctacc tgctgtacca
      661 cgccgtcctt cataacaacg attctggtac ccagtgtcat agcctcggtc tggtcatgtg
      721 taacgtagat aatcgttgtt tccagtctct gatgcagctt ggaaatctca acacgcatct
      781 gtcctcttaa ctttgcatcc aggttggaca gaggctcatc catcaggaat accttaggac
      841 tgcggacaat cgcacgaccc atagctactc gctgtctctg accgccggac agagcctttg
      901 gcttacggtc taacagatgc tccagatcca ggatcttggc tgcatcatga accttcttat
      961 cgatctcatc ctttggagtc tttcttaact tcaggccgaa tgccatgttg tcatatacgg
     1021 acatatgagg atacagagcg tagttctgga ataccatcgc gatatctctg tcctttggct
     1081 caacgtcatt taccagcttg ccgtcgatgt acagctcacc agaggaaata tcctccagac
     1141 ctgcgatcat acgaagggta gtggacttac cgcagccgga cggacctacg aagatgataa
     1201 attccttatc tgcaatctca aggttaaagt ccttaacagc aacgaaaccg ttggggtact
     1261 tcttggtaac atttctaagg gataagcttg ccatattata atttcctcct gaattgggtt
     1321 tttgtaaact catttgcgac ttgcctatat catacatgaa agtccctttt taatccatat
     1381 tgtaaatcca caaagttcaa ttttgtgttt tagctaaatt gtatatcgaa taaaaaaaat
     1441 atccaatctg tcaacacatt ccaatatttt ctgaaaaaat tttgtttatt atgttatcct
     1501 gtcttccttc tcgataaacc cttccccgaa aacttcacga gcgtcgctga caacaaaaaa
     1561 cgcatgcgga tcaaactcac gcacaattga cttgattttt acaatttcct tgggagaaac
     1621 cacgcaaaac agcaggtttt tctcttcttt tgaatacata cccactgctt ttatgctggt
     1681 aactcctctt cccaggcgtt ccatcaccgc ttccgcaatc tctttgtcct gttttgaaat
     1741 gatccaggcc tcttttgaaa acttcattcc ttcgatcagg cgatccgtaa ctctggatac
     1801 gcagacaatg gcgatcatcg catataaaga aggcctaatc ccgaacacaa gagcgccggc
     1861 tgctacaata cctccgtcca aaacctgcat gatctgtgcc gatgaatagt ggcgcagcct
     1921 tccccggatc agcacggcca gcatatccgt tccgcctgtg gtacagcctc caaggaatac
     1981 aagccctgtc cccacgccgc tgagaacacc gccaaaaaga gccgataaaa tcatatcttc
     2041 cttcacaagt cccatctccg gaagcacata caaagccgca gacagcatgg ccgtggagta
     2101 caaagtgcgt ccgataaact gccagcccat catacgaaag gcagccaaaa acagtgggat
     2161 attcaaaacc gtattagtca gccacagagg aacctgccag agttctttta aaataatggc
     2221 cgcgcccgaa acgcctccgg ttaccatgct cacaggatca aaaatatttt taatagcaaa
     2281 cgcgataagg atcgttccta cggtaattgc aagataatcc cggatctgct gtttcttttc
     2341 tgtcataacc aaacctcctt ctgtccggac tttctgtaaa aaaagagcag cccttttaga
     2401 actgccctgt aatctccacc ctgtgatcgt ttgatcctgg tatcctgtaa acacacatca
     2461 atcagatccg gcggatggtc ttttctgtaa tctctatctt tccttccttt aaaaggcggc
     2521 ccacggcacg cttaaaggca tttttactca tgttaaactc cctgagaatc gtttcaggac
     2581 gcgccttgtc attaaaaggg agaactccgt caaattcgtc aatgaccttt aaaacctgct
     2641 ctgcatctgc tcccatctgg acataggctc tttcccttac gcacagatcc agtttgccgt
     2701 cctcacgaac cctcatgact ctggctttta tctgatcgcc ttctctgaaa cggccgtaca
     2761 tctctatttt ggggatcaaa ccataatacc ggttgtccac tgctacaaag gctccgatct
     2821 gttcgttgat ctcatagatc gtaccttcca cccaatcctc tgccttataa ggagactgat
     2881 tgctcatatg ggaatatacc ttcatggtag ctgccagacg gctgctcttg tccacataaa
     2941 ggcagaccag gcatttttct ccccgacgca ccggatggct ctgctcttta aagggaagaa
     3001 gcagatcctt ttccagtccc atatccaaaa atgctcctat ctttcctaca tcctttacct
     3061 ccaaaatggc acattccccc acctgaagct tcggtctggc tgtggtagag atcaagcgat
     3121 ccgaggaatc cttgtataca aaaacctcca ccttatctcc gatctgcgct ccctggggaa
     3181 cctgtttctt cggaagaagg actgctgcct catctcctgc tttttcgctt aagtatacgc
     3241 cgaattcctt tgtcctgacg atctcaagct cctgtacttt tcctaattct atcatatgtc
     3301 tgctcctctt ttactgtccc ggctgctcct gtcctccaag ccataccgct gcataaagct
     3361 ctcccgtttc atttacaagg gccacagtcc actggccctg gcctgccggc gtgattctgg
     3421 gaatcaccgt atcgcccaga cgagctgcct ttttatagtc tgcccggatt tcacggatct
     3481 taagccccac agggatggca tcctttgcaa tctccacata ctgggaatta tttacatgat
     3541 gattggtgtc aagatgatga cggccgatca ctacagctgc tgctgtttcg tatttctccg
     3601 gaatgagaat ctttctgggt gcttctccca gatcctgaga cggctgcggt attccgtaag
     3661 gaaggatatc ttctggctgg atacgcaccg gcatattttt ttctgtatca tagaaaaacc
     3721 agagagaatt tgcccggacc agagggttat tttcctgatc cagcagcaca aaatcacgat
     3781 acccgtaaaa ccctttgctg ccgcagtgcc aggttcccgc agtaatctcc tcccccagct
     3841 tcggataacg gtcaatcacg atccgccagc ctgaaagaag ccatgcgcga tgatgctcct
     3901 tcagatagct tattcccatt ctgccatcct ctgactgaaa ggtactgcaa tcctgcatat
     3961 aattgataat tcccggtacg ctcagccgtc ctgtttcatc tgtttcactg taccggatcc
     4021 tgctcttaaa ctgatacata gctgcctctc tctgctttag gccctcaggc caaatctcct
     4081 gttctgtaag tatatcatac tttcaggcaa aacaaaagcc tccaaatgca tctgcatcta
     4141 aaggcttttt aactgggcta caaggattcg aaccttgaaa atgacggagt cagagtccgt
     4201 tgccttacca tttggcgata gcccactatt taattatgta gagacgccat atgcttctca
     4261 gctgggctac aaggattcga accttgaaaa tgacggagtc agagtccgtt gccttaccat
     4321 ttggcgatag cccattattc tttgccgcat ccgcagctgc gaaaatcatt atacacagaa
     4381 taacaaattt cgtcaagcac tttttataaa aatgctttag ctgggctaca aggattcgaa
     4441 ccttgaaaat gacggagtca gagtccgttg ccttaccatt tggcgatagc ccatcaatac
     4501 aacgctatat attatatatc agctatagag tcacgtcaag tatttttttt atttttgcag
     4561 gattttttga agctttttca tggagcggtt gtattttgac agctctgtag ccagaggaag
     4621 gcccaaagct gaagccactt ccctgtgttt taagcctgct gccgcatgaa gaagcacgat
     4681 ctgccgatcc tcttccctta aggcagccag agcatcagac aaaaccttac gttccggcgc
     4741 ctgttccaat gccgcgtata cctccccctc ttcctcttta tcccattcag aaagctccgt
     4801 ttccctcctg cgctgtctga atctcatgta gcacagatgt ctggctatgg taaacaccca
     4861 ggccaggggc tttccctgcg gttcatagtc ctctgccctg tcccatacgc ataagaaggt
     4921 atcctgcatc agatcctctg cctcatggac atcccttgtg agagacagga taaagctgta
     4981 taccggcccc gcagcctctt tataaaaatc gcggaaggta tccctgtctc cctccgcgat
     5041 cttctgtatc cattcctctt tcatcaatgg tatccccttt cagggatcag ctttttatga
     5101 aaccctactt tactccttct tctgaacggt agctgttttc tactgtaatg gcacaacggc
     5161 agtccggatc tctccggtgc atttcattgc tgatccttgt tctcagtttt cccactgcac
     5221 cgtctttata ttctcttgga accaccagat caaaaatcag acagacattt ttatccttcc
     5281 ctaccatgcg gaaatcatgg aaggacagac gtacatccac atctgccaaa acattttcca
     5341 gcattttcct gtatgtattt aaccgctcat tatctgtttc taccggatcc atatggatta
     5401 ccagcaaaag tcccattttt cgggcggctt ccctctcaat ccggtctacg atcgcatggg
     5461 attctgcaat ttccgcgccc gctgaaacct ctgcgtgaat agaggccatg cttttggatg
     5521 gaccgtagtt gtgaacaatg agatcatggg tacccaaaat accgtcaaac ccttctacaa
     5581 agctggatat ttcctcatag acctcaggag gaatagcttc tccgatcaaa ggcgccagcg
     5641 tatcctttgc aatattgtag cctgcaaaaa gcactgctgc tgacaccata agacctataa
     5701 tgccgtcaat atttacattc cagaggccaa ataccaaaag agacagggct gctgctcccg
     5761 ttgtgaccac atctcccatg gcgtctgcag ccgtagcaag catcaccttt gagttgatcc
     5821 gctttcccag cttccggtta aacgcggaaa gccagaattt cacgcagatt gataaaatca
     5881 gaatcactac cgaaatccat ttaaaggtca taagctccgg gtgaaggatc tttcccacag
     5941 atgttttaaa cagagaaaag cccacctgga tgaccagaaa tgccacgata aaagcagaaa
     6001 tatattcaat tcttccgtgt ccaaaggggt gatccgcatc ggccggctta cctgccatct
     6061 tcacgccgat aaagccgatg atggaggagg ccgcatcgga caggttgtta aatgcatctg
     6121 ccataacaga aacgctgttt accagaagcc cgataaaaag cttggctcca aagagaagaa
     6181 gattgcagaa gattcctacg atactcgcta aaatgccgta ggaagtccgc acctcatctt
     6241 tttccgtatt ttcatatcct tttacaaaat gttttaccag aaattctgtc attttccctc
     6301 accatattca accttttcca gaaaaagccc ctgagccgga gccgtaaacc ctgctgcctg
     6361 gcggttcagg gcttccagaa cggccttcat ctgctcaggc tctctctttc ccagccctgc
     6421 ttcgatgagc gttcctgtaa gaatccgaac catctgctgc aaaaagccat ttcctgtata
     6481 gcggatatgc aggcggctct ctcccgctgc ttcaaattca atggagtcta tccggcggac
     6541 ggttgatttt ttcatccggc tgttgccgca aaaactctta aaatcatggg ttccgataag
     6601 aaacgctgcc gcttcttcca tttttttctg atccagcctg caaccaagac cataataata
     6661 atctctttga aatacgttct ttttagggct catctcaatc tggtaacggt acgtctttct
     6721 cgctgcgctt aaacggctgt gaaagcgttc cggtactgcc tcacaggaaa gaaccgcaat
     6781 atcctcagga aggtaggtgt tgatatattc cagaatctgt aaacttttgt cttcatattc
     6841 ttctgtctgc tttaaatgga aatttgctgt ctgaccttcc gcatgaacac ccctgtccgt
     6901 gcgcccggag ccgaaaacct ctatgggtct gcctgccatc ctggtaagaa tctgctctaa
     6961 ttttccctga atggtccggt ctgtatttcc ctgcttctgc cagccattgt accgggtacc
     7021 gtcataggcc agaatcatct tgtaattcat ctgctctgtc ctttctaact attaagtatc
     7081 ccgcctatta tagtacaaaa aaccggctat tacaacttta ttttaacagt gcaaattcca
     7141 gatccccgat catctgatcc agagcacgga taccgccctc tgcggtatac catacagccg
     7201 gattggacag ataaatcaga tttccattcc gatacgcttc tgttcccatt atcagctcat
     7261 tttccataat ctcctgtgca agcttcgcgc cctctgttcc cactgcagca tcccggtcca
     7321 gtacaaacac ataatccggc tccttttcca caacaaactc aaaggatgct tcatttccat
     7381 gagttgatgt gtcaatctct gcatcaactc caatattctc aaatccggct tcccggctga
     7441 tcagggagca tctgccgtca ttcccaagaa cattaaaacc tccgcttgta accatcccca
     7501 caatcgctgt cttatccgtt gaaaatccag ccagcgctgc aagccgttcc tcaaatccct
     7561 caagcagctg gtctgcctgc tcctccaatc caaatatgga cgcaatgatc ccggcatttt
     7621 ttcggacact ttccaatgtt ccaagctggg tatctatgga cagatacacg acaggagcga
     7681 tcccgcttaa agcatcataa gactttgcca gtctgccgcc tataaaaatc acatccggct
     7741 cactggccat cactgcctcc atatccgcct ccttaatcgt gccgagagct gcaacctgct
     7801 ctttttccat atatttttca agataatcta aggaagtatc ggaagcgccc actacccgtt
     7861 cttccagtcc cagctgttct aaaatgtcca gcgccgccat atccatcaca gcaaccctct
     7921 ttgggtcaaa gggaacctcc aaatccacct ccttcttatc tccatttaag gttttaacgg
     7981 taacaaccga cggttctgcc tttgtaacag cctccgtctg gctttgattt gtctcatttg
     8041 aaccggagca ggcggaaagc cccagcacca taaccgcagc aagtatcaat aatcctgttt
     8101 tcttttttta ataccagatt gaaagaggtt ttccctcaat ctccataatc tcaaactcta
     8161 ccctgtacag ttttgacaaa gtctcttttg tgataacctc ttctactttt ccaaattttt
     8221 ctacttttcc atctgcaaat gcgcagatat aatcagagta aaaggccgca taattaatct
     8281 catgaagcac cagcactact gtttttccca gctcatcaca gagacggcgc accattttca
     8341 tcagattcgt ggcatggtaa atatccaggt tgttagtggg ttcgtccaac agaatatatt
     8401 ctgtatcctg agcaattacc atggcaatat aggctctctg gcgctggcct ccggacagct
     8461 catctatgaa ccgctccctg aaggcctcca gttccatata ggaaatcgcc cggtcaatta
     8521 tccgtttatc ctcatccgtc aaacggcctc cggaataggg aaaacgccca aatgccacca
     8581 gctcttctac ggtaagtttc atctggatct ggttgctctg ggtcaggatt gcaagccgtc
     8641 tggacaactc cctgctcctc cagcctttga tatccttccc ctcaaactcg atcagcccgc
     8701 tgtctctctg aagcagtctt gaaatcattc ccataaccgt agatttgcct gctccgttcg
     8761 ggccgataaa ggaagttacc tttcccctgg ggatctgaaa gctgaccgaa tccaccactg
     8821 tttttccatc atatttttta atcagttctc taattctcac aggccgcgac gcctcctttc
     8881 agtaagcagc agatatagaa aataaatgcc gccaaaaaat gtaataaaaa cacttaccgg
     8941 tactgtatag gtaaatacct gctccacgat cagctgcccg cccaccagga tgatgatccc
     9001 aaaaaatacg gagcctaaaa tcaactggct gtggcggtaa gtctttagaa gctgacggga
     9061 aagattcgca agaatcagtc ctaaaaagga aaccggtcct accatggccg tagccactgc
     9121 gataaataat gccactccaa gaagcagacg acggatgcat ctgtcataat ctacccccag
     9181 attaactgcc tgatcctttc ccaatgtgat cacatccaga agtgctaact ccttcctcag
     9241 agcaaacacc acaagcgcca gcactaaagc cgcaatgacc ataatttcag aatttacatt
     9301 tgcaaagctt gctaccagat ctgcaagaag gctgtcatac tcattggggt ccataatccg
     9361 cgtaagtgca gactgcacac tgccgaaaaa tgaattgagc acagtaccga tcagaagtac
     9421 atagagcaca ttatggccgg ttttcctaaa cagtgtgctg taaatcaccg ttgcagcggt
     9481 tcccatgatc aataaatcca ctccaaaggc tgcctttgca ttaacagcca gtatactgcc
     9541 tgatcctgct gcaaagataa ctgccgtatg aatcagcata tagagggaat tcattcccaa
     9601 aaggcaggga gtcacaatgg tattattgat aacggactga aataccagag atgccccgcc
     9661 aatggcaaat gctgtgatca tcatggcaaa cagtttggga gcacgaatac gcatggcata
     9721 tgcaaacagc ttctcatttt caaaatccac atttacaagc atataaaggg ctgccgcgcc
     9781 aagcgccaga actgccataa ggagcagccc ggcctgggtt ttatgagcgt tcttctgcat
     9841 cactgtattt tcctcccttt ccctgcttcc ataactctca tattttttat ctgaggaagc
     9901 ttgaatacaa cggatttttt tccgtacttc agcctgtaaa atataatggc aatgaaaagg
     9961 atacttccta caatccctac aatcaattcg attggaagtt cataaggggc aatcacgctt
    10021 cgcccaatca tgtcacaggc cagaacaaac acggctccaa acagtgcggt atctgtcagc
    10081 gtacctctga tcctgtcccc ctttatcatg gaaatcacat tgggaacgat aaggccgata
    10141 taggaaatgg aaccgaccac cactacaata gaggctgtga tcatagctga aatggtaagc
    10201 cccagaaaca ggatcaggtg atagggcaca ccgagatttt ccgaaaaatc acgtcccatt
    10261 cccacaatat tgaagtgatt ggcaaaaaga aatgccagga ttaccagcgg gactgtcaga
    10321 taaacgattt cataccttcc tcttaaaacc atggaaaaat gtcccaccag ccaggaagat
    10381 aatgcctgtg tcatttcata tttatatgcc agatagctgg tcattccgga aatcacattg
    10441 ttaaacataa ttcccaccag cggaaccata accgcatcct tgaacggtat cctctgaata
    10501 aaccagacaa aaatccaggt gcctgccaac gctgctccga atgcaaacag cgcccgtccc
    10561 caaagtgtag aacctggcat aaacaaaagt gccagaagga tgccaaactg cgctgatgaa
    10621 atagtagctc ctgtggtagg agatacaaat ttattcatac aaagctgctg catcatcagc
    10681 cctgctacgc tcattcccac acctgtgcag agaatcgcaa gaaggcgggg aagacgggaa
    10741 atcagtaaaa tctcaagctg ctctgcccct cccgtaatca aatgggatgg cgtaagatca
    10801 attactccaa tgaacaaaga tgccgcagac aggcataaaa gagccagggc cagaatcacc
    10861 gttaaaaatc gactattaat agtagtacct ccttttttca gttagttcta tctaactttt
    10921 gaataaaata aaatgtgtat ctgctgctga ccttttaaag tatcgcagtc gatgcacatg
    10981 agcacaatta gaatacacta acttttattt tttgtcaaga attatcttgc agaactcggc
    11041 agctgttttc caaatgccta tagctttcct ttgtacatgg gtgttcttac ttcaatcaga
    11101 tttccatcca agtcataaaa ccgcaccatc ttctgtcccc agctgtactc tgtaagccga
    11161 ttgacatact gaatcgacgg atattgtgtt tccaatttac agataaaagc ttctatctcc
    11221 ggctcttcaa aatacaactc gcagaaatta ttttcaggaa taatctcctt tcccagagct
    11281 tctctccaga ttttttcatc ctgaagcaca aggccttccg ttaaaatcat atttccttca
    11341 ctattaagca cggtctgaag gccaaagagt ttgtgataaa attgccttga tttttcgata
    11401 tcttttactg caagaagaat gttcctcagc ttcatggtcg atcttccttt cttcctgtct
    11461 acaactgtct accaattttc agagaatgca aaatggctga aattcttgaa aaatcaagca
    11521 attccagcca ataaaaagag cgcgagacgg gattcgaacc cgcggccccc accttggcaa
    11581 ggtggtgctc caccactgag ccactcgcgc atttagtaca tttcacatat accttcaaaa
    11641 ccacatactg aacaaccaag ccacaaccgt tttccctaaa ccttctggtc aagccctcga
    11701 ccgattagta tcagtcagct c
//
LOCUS       DBMDUH010000078        11669 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_2791_length_11669_cov_5.609094, whole genome shotgun sequence.
ACCESSION   DBMDUH010000078 DBMDUH010000000
VERSION     DBMDUH010000078.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 11669)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11669)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11669
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2791_length_11669_cov_5.609094"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(204..2027)
                     /locus_tag="ACZPCG_07850"
     CDS             complement(204..2027)
                     /locus_tag="ACZPCG_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009251790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIR1285010.1"
                     /translation="MNLLTIEHLTKSYTERLLFDDTAFSINEGEKVGLIGINGTGKST
                     LLRIAAGLEEPDEGSVVKGRSLYIRYLPQIPEFQDGETVLEAIVRENENEPHFSSKEE
                     LKAAARSMLNRMEVPDCEAKIDTLSGGQRKRVALASVLLSTADLLILDEPTNHLDSGM
                     AEWLEAYLKAFKGALLMITHDRYFLDSVTSRIVELDKGKLYSYQGGYEKYLELKAERI
                     EMAEATERKRQSILRTEIAWMQRGARARSTKQKAHIQRYEALRDQEGPQYDRNVQLES
                     IASRLGRTTVELKDISKSYGDKVLLKDFTYIFLKNDRVGIIGPNGSGKSTLMKIIAGW
                     VQPDSGTTEIGQTVKLGYFSQENEAMDESLRVIDYIKNAAEYVKTKDGSISASQMLER
                     FLFPSSVQYTVIGKLSGGEKRRLYLLRILMEAPNVILLDEPTNDLDIQTLVILEDYLD
                     TFPGIVITVSHDRYFLDRVVNRIFAFEGDGRVQQYEGGYTDYQNACLKRAEAEGGQPG
                     GGQEGKTEVRTNGKTQRTRGPQKLKFSYKEQRDYETIEGEMADLEERISSLEQEIQAS
                     AHDFVKLNQLMAEKEEAEAALEEKMERWMYLEELAAKIAEQ"
     gene            complement(2053..2808)
                     /locus_tag="ACZPCG_07855"
     CDS             complement(2053..2808)
                     /locus_tag="ACZPCG_07855"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HIR1285011.1"
                     /translation="MKKLISWNVNGLRACVGKGFLDFFKEADADVFCIQESKLQEGQI
                     DLELPGYFQYWNYAEKKGYSGTALFCKEEPVSVSYGLGIEEHDHEGRVITAEFGDYYV
                     VTCYTPNSQDGLARLDYRMEWEAAFLAYLKQLEEKKPVIFCGDLNVAHKEIDLKNPKT
                     NRKNAGFTDEERGKFTELLDAGFIDTWRYFNPDTEGVYSWWSYRFKAREKNAGWRIDY
                     FCTSEALKDRLVSAGIHTEVFGSDHCPVELVIE"
     gene            complement(2838..3323)
                     /locus_tag="ACZPCG_07860"
     CDS             complement(2838..3323)
                     /locus_tag="ACZPCG_07860"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HIR1285012.1"
                     /translation="MEKIASFTIDHIRLQPGVYVSRKDQVGDQVITTFDLRMTSPNEE
                     PVMNTAEMHTIEHLAATFLRNHKEYGSRTIYFGPMGCRTGFYLLLAGNLASREILPLM
                     VELFAFIRDFEGEVPGASARDCGNYLDMNLGMAKYLAARYLKVLEQIDSYPESRLEYP
                     Q"
     gene            complement(3464..5101)
                     /locus_tag="ACZPCG_07865"
     CDS             complement(3464..5101)
                     /locus_tag="ACZPCG_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009219319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIR1285013.1"
                     /translation="MISANNISLRVGKKALFEEVNIKFTEGNCYGLIGANGAGKSTFL
                     KILSGQLEPTSGEIAITPGQRLSFLQQDHFKYDEYTVLDTVIMGNARLYQIMKEKEAI
                     YMKEDFTDEDGIKAAELEGEFATMNGWEAESDAESLLNGLGIGPEFHYSQMGNLLGAQ
                     KVKVLLAQALFGNPDILLLDEPTNHLDLDAIAWLEEFLINFENTVIVVSHDRYFLNKV
                     CTQIADIDYGKIQLYAGNYDFWVESSQLIIKQMKEANRKKEEKIKELQEFIQRFSANA
                     SKSKQATSRKRALEKIELDDIKPSSRKYPYIDFRPSREIGNEVLTVEHLSKTIDGEKI
                     LDDISFILGREDKVALVGPNERAKTVLFKILAGEMEPDEGSYKWGLTTTQSYFPKDNS
                     AEFANDDTIVEWLTQYSPEKDVTYVRGFLGRMLFAGEDGVKKVKVLSGGEKVRCMLSK
                     LMISGANVLMLDEPTDHLDMESIDALNRGMIKFPGVMIFSSRDHQIVQTTANRIMEII
                     NGKLIDKITTYDEYLESDEMARKRFTFTVAESDEQDD"
     gene            complement(5578..6726)
                     /locus_tag="ACZPCG_07870"
     CDS             complement(5578..6726)
                     /locus_tag="ACZPCG_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HIR1285014.1"
                     /translation="MKITDVKLIYAKHYLFVKIYTDAGIVGLGEAGNWGYLDATAAAI
                     QKFSSYLIDKDPFRIEDFNQNFYRSVYFRGSVIMSAISAIDIALWDIKGKALGVPVYE
                     MLGGKVREKVRVYASIMTKAESLTELSEGYRALKEQGFTAAKIFVNGPVKTRNDGRDE
                     FFSGRIEEELEKVRVCREAVGSDFDFVLEAHRGMNVPEAITFGNEVAKYRPMVFEDPI
                     TPDNPDAMAEVAAKVPVPIATGERFINLKEFETVMARHGCQYIRPDVCAVGGITTSKK
                     ICALAEAHDVLVIPHNPLGPVSTAACLQLCASIPNLGIQELPGFCLNGAEDAMVKEPL
                     HFENGCLMIPDAPGIGVELSENAEELYPANERGSNAAKRAFDGSVKDW"
     gene            complement(6726..7637)
                     /locus_tag="ACZPCG_07875"
     CDS             complement(6726..7637)
                     /locus_tag="ACZPCG_07875"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019474.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR1285015.1"
                     /translation="MTREEMIEAAKKIRLKDNFGVVSKEELETLLGEQREFYLDTDNG
                     CHVHIYELKLSKSHQIKCPLLINFHGGGFIKGRLDRDRRYCSYLLEHFDCIIWDVDYC
                     LAPENPYPAALNECYGVAAWAFLHGEEMGIDVNRIAFAGHSAGANLTAAICIHDAAFH
                     KLHPCALLMEYMPADHTADPFVRLNEEQKKDPWFVARAEREAEYMEFYCEKEDAVKAS
                     CSPLLASDEQLSNFPDCLVLSAGHDTLCKETEKFAARLIEAGVTVTAKRIPEAMHGFT
                     TNRTDGWERALKLHCEFLNYHFKEIVD"
     gene            complement(7676..8968)
                     /locus_tag="ACZPCG_07880"
     CDS             complement(7676..8968)
                     /locus_tag="ACZPCG_07880"
                     /inference="COORDINATES: protein motif:HMM:NF013132.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIR1285016.1"
                     /translation="MSTQMILCMVIFACTLVSYMMNKIPMWLTALLSLCALFIAGCVD
                     ANGALAGFANTNTILMATMFVVAAGFRRTSMVDKMCNGILRMTKGSFKMAYLGYLILA
                     AILTNFIASPMVVYAIVSPLLVALCDKTENSRSMYMFPIMVVCVACSGILPLPTAIQT
                     AGQFTGFMESYGFSGMTFEPIYFLMGAWPVLFVVIGWALLLAPKFTPKQPLIAIEARE
                     QKTSGTQSSLSPMVDKIGIAIFFMTIFALIFSAQIGQPTWLIALLGSLLMVLFGVVDQ
                     KNALRDIPWDMLMLYVGALALGTALTNTGAGEMIGNALAAAVGGTHNNYVLGALFFII
                     PFIVTQFMLNRAVSAVFVPICLLTCSSLGANPIGLMILVQAGSLTAFLTPMATPAVPM
                     CMADGGYTLPSLVKSGALISLFLPIVYIFYTMTVFPAF"
     gene            9355..10287
                     /locus_tag="ACZPCG_07885"
     CDS             9355..10287
                     /locus_tag="ACZPCG_07885"
                     /inference="COORDINATES: protein motif:HMM:NF024242.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIR1285017.1"
                     /translation="MKEAYFEFQFPRDNLYLSIFSHKIMNYRYHWHPTDYELNILLSG
                     TQEYFLDTKTYTLSENDVILLPPGIGHASHSWDGSAYSLVIHFSKTAFKPYVKKGYML
                     NFPECRSSKEIQQEPGFSRLRFYAAQIMLAAQSGETYSQLEIKASMELLISTLGSMFS
                     PQVIKAPDEEAERHQETVKKLLSYIEAHYREKLTLEELAESVQYNRTYLSTLFRNTVG
                     VNFYEYLMRVRFQKALSELAETNKNLTDIALDNGFSDLKSFNARFRSVLHCMPSEYRA
                     KLNPAYIMPAEQRNFISWEEPHLKNKLLEYAHLG"
     gene            complement(10735..11361)
                     /locus_tag="ACZPCG_07890"
     CDS             complement(10735..11361)
                     /locus_tag="ACZPCG_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285018.1"
                     /translation="MRKQVYMTAVAVMAGVLLASGCSKNQDEMPSSLVPQTETATESE
                     ASFETETTPAQTEEPAEAGRYHMLQGTVIEEKEDGSAFTLLADDGKEYEISLSDIRDV
                     ETEIAEDIQIAIAYIGEPLGKLEDVDLVVALPEQEEWTISTEEGTTTANAMSSFAMKT
                     ADGRELSFLKDNCPIEDGALSEDSGDSIWVTYVTSEGMNFPVEIEKGE"
     gene            complement(11563..>11669)
                     /gene="glnQ"
                     /locus_tag="ACZPCG_07895"
     CDS             complement(11563..>11669)
                     /gene="glnQ"
                     /locus_tag="ACZPCG_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586785.1"
                     /note="similar to ATP-binding component of ABC
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HIR1285019.1"
                     /translation="FMAGGVIVEEGTPEEVFSSDNERTQSFLGRYGTI"
BASE COUNT         3108 a         3125 c         2189 g         3247 t
ORIGIN      
        1 tcatggggga aggggattct ggcgacaggc tgcctgctcc cttttttgaa gagaagcagc
       61 ccgcagcttt ctgcctccga aataaaacac acatcaaagc cgtccccatt cgccgccaca
      121 ccaggcggat agatccggct tttgtctgcc accgcaatat aatcctgttt catctatata
      181 ccgcctttca ctgaattcat actctactgc tctgcaatct ttgcagccag ctcttccaga
      241 tacatccacc gctccatctt ctcctctaaa gcggcttccg cctcctcctt ctctgccatg
      301 agctggttca attttacaaa atcatgggct gaagcctgga tttcctgttc cagagaggaa
      361 atcctctcct ccaggtctgc catctcacct tcgatggttt catagtctct ctgctcctta
      421 tagctgaatt tcagcttctg ggggccgcgg gtacgctggg tttttccgtt tgtacggact
      481 tcggttttcc cttcctgtcc gcctcccggc tgtcctcctt ctgcttctgc ccttttaagg
      541 caggcattct gataatccgt atacccgcct tcatactgct gtacccttcc atcgccctca
      601 aaggcaaaga tcctgtttac cacgcggtcc aggaaatacc ggtcatggga tacggtaatc
      661 acaatccccg ggaaggtgtc cagataatcc tccaggatca ccagcgtctg gatatccagg
      721 tcattggtag gctcatccag aaggatcaca ttgggcgctt ccatcaggat acggagcaga
      781 tacagcctgc gcttctcccc tccggagagc tttccgatca ccgtatactg aacactggac
      841 ggaaataaaa accgctccag catctggctg gcgctgatgc ttccatcttt tgttttcaca
      901 tattctgcag cgtttttaat ataatcaatg acgcgcaggc tctcgtccat ggcttcattt
      961 tcctgggaaa aataacccag ctttaccgtc tgtccgattt ctgttgtacc ggaatccggc
     1021 tgtacccagc ctgcaatgat cttcatcaga gtagactttc cgcttccgtt ggggccgata
     1081 attcccacac ggtcattttt caggaaaata taggtgaaat cctttaagag caccttatct
     1141 ccgtaggact tggaaatatc cttcagctct accgtagtcc gtcccagacg gctggcaatg
     1201 gactctaact gtacatttct gtcatactgg ggcccctcct ggtcacgcaa cgcctcatag
     1261 cgctggatat gggccttctg cttggttgaa cgggctctgg cccctctctg catccaggca
     1321 atctctgtcc ttaaaatgga ctggcgcttg cgctctgtgg cctctgccat ctctatccgc
     1381 tctgctttaa gttccaggta tttttcgtat cctccctgat agctgtagag ctttcccttg
     1441 tccagctcca cgatccggct ggtcacactg tccagaaaat accggtcatg ggtgatcatc
     1501 aaaagagcgc ccttaaaggc ctttaaatat gcctccagcc actctgccat gccgctgtcc
     1561 agatggttgg tcggctcgtc cagaatcagc agatctgccg tacttaaaag aacgctggct
     1621 aaagctaccc tctttctctg tccgccggaa agagtatcta tctttgcctc gcagtccggc
     1681 acctccatac ggtttaacat agagcgggcc gctgccttta actcctcctt tgaggaaaaa
     1741 tgaggctcat tttcattttc acgcacaatg gcctccagca cggtttctcc atcctgaaat
     1801 tccgggatct ggggcagata ccgaatatac aggctgcgtc ccttcaccac cgaaccctca
     1861 tccggctcct ccaagcctgc tgcaatccga aggagcgtgg actttccggt tccgttgatt
     1921 ccgatcaggc cgaccttttc cccctcattg atggaaaatg ccgtatcgtc aaacagcaga
     1981 cgttcggtat atgatttcgt taaatgctca atggtaagaa gattcatgga tccgtttcct
     2041 ttccctcatt tgctattcaa tcaccagttc taccggacaa tggtccgagc caaatacctc
     2101 tgtatggatt cctgcgctta ccagacggtc ctttaaggcc tcgctggtac agaaatagtc
     2161 gatgcgccac ccggcattct tctctctggc cttaaaacgg taggaccacc aggaatacac
     2221 gccctctgta tccggattaa aataacgcca ggtatcaata aagccggcat ccagaagctc
     2281 tgtaaatttt ccccgctcct catcggtaaa accggcattt ttccggttag ttttgggatt
     2341 tttcaggtca atctctttgt gggccacatt cagatccccg cagaaaatca ccggcttttt
     2401 ctcctccagc tgcttcagat aggctaaaaa tgctgcttcc cattccatgc ggtaatccag
     2461 ccttgcaagg ccgtcctgag aattgggagt atagcaggtg acaacgtaat aatcgccaaa
     2521 ttcagccgtg atcacacggc cctcatggtc atgctcctca atccccagtc cgtatgatac
     2581 agaaaccggc tcctccttac agaataaagc cgttccggaa taccccttct tctctgcata
     2641 attccaatac tgaaaatacc ccggaagctc aaggtcaatc tgtccctcct gaagcttgct
     2701 ctcctgaata cagaatacat ctgcatccgc ctccttaaaa aagtccagaa aaccctttcc
     2761 cacacaggcc cgcaggccgt ttacattcca tgaaatcagt tttttcatac agaactcctt
     2821 tcctgatcca aaaagcccta ctgcggatac tccagtctgg attccggata gctgtcaatc
     2881 tgttccagca cctttaagta tcttgcagcc agatactttg ccatccccag attcatatcc
     2941 agataattgc cgcagtccct tgcagaagcc ccaggcacct caccctcaaa atcccggata
     3001 aaggcaaata gctccaccat cagaggcaga atctcccttg aagccagatt gcctgccaga
     3061 agcagataaa aaccggtacg gcagcccata ggaccaaagt aaatggtacg gcttccatac
     3121 tccttatggt ttcttaaaaa ggtagctgcc agatgctcaa tggtatgcat ctccgccgta
     3181 ttcatcaccg gttcctcatt gggagaggtc atacgcaggt caaaggtcgt aatcacctga
     3241 tcccctacct gatcctttct tgaaacatat acccccggct gaagccggat atggtcgatg
     3301 gtaaaacttg caattttttc cattttgaat cttctccttt tcttcattat ctcgctgcct
     3361 gcggtgtgca ctgcacacca caaaccgcct cttcttccat ccgaaaaggc aggacccttt
     3421 gtccagatcc tgcctccatc ttgtgccaaa acgcacctta taatcaatca tcctgctcgt
     3481 cgctttcagc aacggtaaat gtaaatctct ttctagccat ctcatcgctt tccagatact
     3541 catcatatgt ggtgatcttg tcaatgagct ttccattgat gatttccatg atgcggttgg
     3601 ctgttgtctg tacgatctga tggtcacggg aggagaagat catcacaccc gggaacttga
     3661 tcataccacg gtttaacgca tcaatagact ccatatccag gtggtctgta ggctcgtcaa
     3721 gcatcagtac atttgcaccg gaaatcatca gcttggacag catacagcga accttctcac
     3781 caccggaaag aacctttacc ttctttacac catcctcgcc cgcaaaaagc atacggccca
     3841 ggaaaccacg cacatatgtt acatcctttt caggagaata ctgggtcagc cactctacga
     3901 tagtatcgtc atttgcaaac tcagcggagt tgtccttggg aaaataagac tgggtagttg
     3961 tcagacccca cttatagctt ccctcgtcgg gttccatctc acctgcaaga atcttaaaga
     4021 gaacggtctt tgcacgctcg tttggcccta ccagagcaac cttgtcctct cttcccagaa
     4081 taaaggaaat gtcgtcaaga atcttctcgc cgtcaatggt cttgcttaaa tgctctacgg
     4141 tcagaacctc atttccgatc tcacgggagg gacggaagtc aatgtaagga tactttctgc
     4201 tggatggctt aatatcgtcc agctcgatct tttccagggc gcgcttacgg gaggttgcct
     4261 gcttggactt ggaagcattt gcagagaaac gctggatgaa ctcctgcagc tccttaattt
     4321 tctcttcctt cttccggttg gcttccttca tctgcttgat gatcagctgg ctggactcta
     4381 cccagaaatc gtagtttccg gcatacagct gaatcttgcc atagtcgata tctgcaatct
     4441 gtgtgcagac cttatttaag aaataacggt catgggatac cacgataacg gtattttcaa
     4501 agttaatcag aaactcttcc agccatgcaa tggcgtccag atccaggtgg ttggtaggct
     4561 cgtccagaag caggatgtca ggatttccga ataatgcctg tgcaagaagc acctttacct
     4621 tctgtgcgcc cagaagattt cccatctggg aatagtggaa ctcagggccg atgccaaggc
     4681 cgtttaacag gctctctgca tcagactctg cttcccagcc attcatggtg gcaaactcgc
     4741 cttccagctc tgccgccttg atgccgtcct cgtctgtgaa gtcctccttc atgtagatgg
     4801 cttctttttc cttcataatc tggtacagtc tggcatttcc catgataacc gtatcaagca
     4861 ctgtatactc gtcatatttg aaatgatcct gctgtaagaa ggaaagtctc tgtcccggtg
     4921 taatggcaat ctcaccgctg gtcggctcta actggccgga cagaatcttt aaaaatgtag
     4981 actttcccgc accgtttgca ccgatcagtc catagcagtt accctcggtg aatttgatat
     5041 tcacttcctc aaacaatgcc ttcttgccta cgcgaaggga gatattattt gcactaatca
     5101 tgtctcttac ctttctttgg aatcttattt ttatcgttcc tcgtcaaaag aggggtcaga
     5161 atctggcccc tctttttatg tttctctcaa tatcagattg tacagggaaa acagggtttt
     5221 tgcaagggga ttttcggaaa aatcaaaaaa tacgtaaatt gaaaaagtga aatctacctt
     5281 tttgttaaaa gctagacttt agtcttacat aatgatagaa gtgactcttt catgcgaaag
     5341 aatacttccc ctttgactgg ttgactattt tacacccttg tagctggaat actgtcaatg
     5401 gcttgttgtt tacgatacca agtaccattg acagagttcc cgctacaagg gtatcatata
     5461 tccccaacag tcaaaaatat tacaataact ataattccat atagaattta cccttacata
     5521 cagactttac tttttcaatt cacttctttt tctcttttta agagaaaagt ttctgcttta
     5581 ccagtctttt acagaaccat caaaagctcg ctttgccgca ttacttcccc gttcatttgc
     5641 cggatacagt tcttctgcat tttctgaaag ctcaacccct atgcccggcg catccggaat
     5701 catcagacag ccattttcaa aatgaagggg ctcttttacc atggcatcct ctgctccatt
     5761 taagcagaag ccaggaagtt cctgaatccc aagatttgga attgaagcac aaagctgcag
     5821 acacgcagcc gttgataccg gcccaagcgg attatgagga atgacaagta catcatgagc
     5881 ctccgccaat gcacaaatct ttttgctggt tgtaatcccg ccaacagcac acacatccgg
     5941 tcggatatac tggcacccat gccgtgccat aaccgtttca aactccttca gattaataaa
     6001 acgctcacct gttgcaatcg gtaccggcac cttagcagct acttctgcca ttgcatccgg
     6061 attatccggc gtaatcggat cttcaaatac catcggacga tattttgcca cctcatttcc
     6121 aaaggtaatg gcctccggta cattcattcc acggtgtgct tccagaacaa aatcaaaatc
     6181 tgaaccaaca gcctccctac atacccgaac tttttccaat tcttcttcaa tccgtccaga
     6241 gaaaaattcg tctcgtccat cgttgcgtgt cttaaccggg ccgttcacaa aaatcttggc
     6301 tgcagtaaat ccctgctcct tcaaagcccg atatccttct gaaagttctg tcaaagattc
     6361 cgcctttgtc ataatagagg cataaacacg aactttttca cgcacttttc caccaagcat
     6421 ttcataaacc ggtacgccca gggcctttcc ctttatatcc caaagagcaa tgtcaatggc
     6481 agaaatcgca ctcataatca cggatccgcg aaaataaaca gaacgataaa agttctgatt
     6541 aaaatcttca atccgaaatg gatctttatc gatcaaataa gaagagaact tctgaatggc
     6601 agcagcagtg gcatccagat atccccaatt tcccgcttca ccaagtccaa caattccagc
     6661 atctgtataa atcttcacaa acagataatg cttcgcataa atcagtttca catctgtaat
     6721 cttcatcaat ctactatctc cttaaaatga taattcaaaa actcgcaatg cagcttaagc
     6781 gcacgctccc atccatctgt acgattcgtg gtaaatccat gcattgcttc cgggatccgc
     6841 tttgctgtta ccgttacacc ggcttctata aggcgtgctg caaatttttc tgtttctttg
     6901 caaagtgtat catgtccagc agataaaaca agacagtctg ggaaattaga gagctgttca
     6961 tctgaagcta aaagcggcga acagcttgct tttactgcat cttctttttc acagtaaaat
     7021 tccatatact ctgcttctct ttccgctctt gcaacaaacc aaggatcctt cttttgctcc
     7081 tcatttaaac gcacaaacgg atctgccgta tgatcagcag gcatatattc catcagcagc
     7141 gcacacggat gaagtttgtg aaacgccgca tcatgaatgc atattgctgc tgtaagattt
     7201 gcacccgcac tatgtcccgc aaatgcgata cggttcacat caatccccat ttcctcccca
     7261 tgcagaaaag cccatgccgc cacaccataa cactcattta acgctgctgg ataaggattt
     7321 tccggcgcca gacagtaatc tacatcccat attatgcaat cgaaatgctc cagcaaataa
     7381 gagcaataac gccgatcccg atcaaggcgg cctttgataa aaccgcctcc atgaaaatta
     7441 atcaacagag ggcattttat ttgatgtgac ttactcaatt ttagttcata aatatgaaca
     7501 tggcagccat tatctgtatc aagataaaac tctctttgtt cgcccagaag tgtttccaac
     7561 tcttctttac tgaccacacc aaaattatcc ttaaggcgaa tcttcttcgc tgcttcaatc
     7621 atttcttctc ttgtcatcca aacacactcc aatcatatcc ctgactctta aaatatcaga
     7681 atgccggaaa tactgtcatt gtatagaaaa tatacacaat tggaaggaac agggaaatca
     7741 gtgcaccact ctttaccaga gagggaaggg tatagccgcc gtctgccata cacatgggaa
     7801 ctgccggagt tgccataggt gttaaaaatg ccgttaaact tcctgcctgt actaaaatca
     7861 tcagtccgat gggattcgcc cccagagagg aacaggttaa aagacaaatc ggtacaaata
     7921 cggctgatac tgcacggttc agcataaact gtgtcacaat aaacggaata ataaagaaca
     7981 aggcaccaag cacatagttg ttatgggttc cgccaactgc cgctgcaagc gcatttccga
     8041 tcatttcgcc agctccggta ttggtaagtg ccgtacccag agcaagcgct ccaacataaa
     8101 gcatcagcat atcccacgga atatcgcgca gtgcattttt ctgatctacg acaccgaaaa
     8161 gtaccatcag caaactgcca agcagtgcga tcagccatgt aggctgccca atctgtgctg
     8221 agaagatcaa agcgaaaatc gtcataaaga aaatggcaat accgatttta tctaccatag
     8281 gggaaaggga agactgagta ccggaagtct tctgctctct tgcctcaatt gcaataagag
     8341 gctgctttgg agtgaatttc ggtgcaagca gtaatgccca tccaattaca acaaacaaaa
     8401 ccggccaggc tcccatcagg aaataaatgg gttcaaaggt cataccgctg aatccatagc
     8461 tctccataaa tccggtaaac tgtcctgcag tctggattgc tgtgggaaga ggaaggatcc
     8521 cgctgcaggc cacacatacg accatgattg gaaacatata catagagcgg ctgttttccg
     8581 tcttatcaca aagagcaaca agaagcggag atacaattgc gtaaactacc atcgggctgg
     8641 caataaagtt tgttaaaata gctgccagaa tcaaatatcc caaataagcc atcttaaaag
     8701 accctttcgt catacgcaaa attccgttac acattttatc caccatactg gtgcggcgga
     8761 agcctgctgc aaccacaaac atagtcgcca tcaaaatcgt gttggtattg gcaaatccag
     8821 ccagtgcgcc atttgcatct acacatccgg caataaaaag cgcacatagg gacaacaacg
     8881 cagtcagcca catgggaatt ttattcatca tgtaactgac cagggtacat gcaaagatta
     8941 ccatacataa tatcatttgc gtgctcataa cttcattcct cgctttcttt tgcagttcct
     9001 tttcggagtc tgttttctgt taattctaat gtaacaaaaa ccacaaaatt gtgcgctgtt
     9061 attattctgc acttttaaat gccatttcta tctttgtaaa aaaagcccct gtaaaacagc
     9121 tatatacaaa agaaagaaat ccaaatattt tccctattac ttttgcattc atcaagttct
     9181 tcttttttgt gcattttgtc caataaaatc catatatcga aattatgaat tatcaaatat
     9241 aggttgacaa tactaaaaat ctacattaaa ttaagaaaca gtaaacatat ttaaaaatct
     9301 tatccgaaga atataacaga ggaaatattc agatttatga aaaggagaat tttcatgaaa
     9361 gaagcctatt ttgaatttca atttccacga gacaacttat atttatctat tttttctcat
     9421 aaaatcatga actaccgcta tcactggcat ccaactgact atgagctgaa tatcctgctc
     9481 agtggtacac aggaatattt tctggacaca aaaacctata cgctttcgga aaatgatgta
     9541 attctgcttc ctcctggaat tggccatgcc tctcactcat gggatggcag tgcctactcc
     9601 ctggtaatcc atttttcaaa aactgcattt aaaccttatg taaagaaagg atatatgctg
     9661 aatttcccag aatgcagaag cagtaaagaa atacaacagg aacctgggtt ttcccgcctg
     9721 cgcttctatg ccgcacaaat catgctggct gcccaatccg gtgaaaccta ttctcaatta
     9781 gaaatcaaag caagcatgga gcttctgatt tccactctgg gcagtatgtt ttctcctcaa
     9841 gtaatcaaag cacctgacga agaagctgaa cgccatcagg aaactgtgaa aaaacttctt
     9901 tcctatatcg aagcccacta ccgcgaaaaa ctgacattag aggagcttgc ggaatctgtt
     9961 caatacaacc ggacgtatct ttctactttg tttaggaata ctgtgggcgt taacttttat
    10021 gaatatctta tgcgcgtccg ctttcaaaaa gcactcagcg aactggcaga aacaaacaaa
    10081 aatctgactg acattgctct ggacaacggt ttttctgatt taaaatcctt taacgcccgt
    10141 ttccgtagtg tcctgcactg catgccgtcc gaataccgcg caaagctgaa tcctgcatat
    10201 attatgccgg ccgaacaacg caacttcatt tcctgggaag aacctcatct gaaaaacaaa
    10261 ctgctggaat atgcgcattt aggatagctg aaaaaaaggg gagctcttcc tgtaagattt
    10321 aggcttttgg aagccttgag tcaaaacagg aagaactcct cttttccgtg tttcctattt
    10381 tagggcatag ttatcccttg tctggatttt ttatccttct tttctgtcta agctgggatg
    10441 caagcacatc agcagctcac aaaattatca ctttaaacct tttttcgcct gtgatttcat
    10501 aaactgtctt ctgtatttcc ctctggttgc acacatcaat agaaaatcca tgccaaaacg
    10561 ctccatattc ctttccagaa tatgaacact gtggtctaac tccacccaaa aaacctctgt
    10621 ttcgcataat ttctccattt gagccaattc cattctaccc cttagaccca tcccttacaa
    10681 aaaaccattt tttatttata tatggaaaaa tctgaggttc atcatttcac tctcctactc
    10741 ccccttctca atctccactg gaaaattcat cccctcagag gtcacatacg tcacccagat
    10801 cgaatctccg ctgtcctctg acagcgcacc gtcctctatg gggcagttat cctttaaaaa
    10861 gctcagttct cttccgtccg ctgttttcat cgcaaaggaa ctcatggcat tagccgtggt
    10921 ggttccttcc tctgtggaaa tcgtccactc ttcctgctcc ggcagggcca ctaccagatc
    10981 cacatcctcc agttttccaa gcggctcgcc aatataggca atggcaatct ggatatcctc
    11041 tgcaatctcc gtctctacat cccggatatc gcttagggaa atctcatact ctttcccatc
    11101 atctgccaga agcgtaaagg cagaaccatc ttctttttcc tcaatcactg ttccctgaag
    11161 catatgatag cgcccggctt ctgctggttc ctctgtctgt gccggggttg tctctgtctc
    11221 aaaggatgcc tccgattctg tggctgtctc tgtctgcgga accaatgaag acggcatctc
    11281 atcctgattt ttgctgcatc ccgaagcaag aagaactcct gccatcactg ccacagctgt
    11341 catatatacc tgttttctca tatttttacc tccgatcttc cgtcaaaaaa cagaatttcc
    11401 atacttttac ccagatgtta ccatccctgt ctgaaaaata caagaagttt tcccttacag
    11461 tttcttaata ttatgcacgg aacgcgccta ttcctcaaca aaaacagaca gcgccggagg
    11521 atgcttttct cctcaggcac tgcctgccat ttattttctc tatcaaatcg ttccatatct
    11581 ccccagaaag ctctgcgtcc gctcattatc tgaggaaaat acctcctctg gtgttccctc
    11641 ttccacgatc acccctccgg ccataaaga
//
LOCUS       DBMDUH010000079        11611 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_2808_length_11611_cov_5.087750, whole genome shotgun sequence.
ACCESSION   DBMDUH010000079 DBMDUH010000000
VERSION     DBMDUH010000079.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 11611)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11611)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11611
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2808_length_11611_cov_5.087750"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="ACZPCG_07900"
     CDS             complement(<1..681)
                     /locus_tag="ACZPCG_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR1285020.1"
                     /translation="MDKSKKIMIGAIGTVVVAGGVIIGLILGRTPPKADLSTIHTEAS
                     TEAPAENKPQETKAPETEPSKAPEESVTAISAKVMTYTSGKVSIEYPAIEQMEDEEKQ
                     SRINEHLKANALSILDVYEINESADTLSVSCEIISADPKRLTAVYTGELSIEGGAHPS
                     RIFYTNTLNLNQMKDMGLNDFTDAYTMAGYVLSEDVRFPKLNAEETSAALEYRSTQSM
                     EALTAVFEN"
     gene            858..1055
                     /locus_tag="ACZPCG_07905"
     CDS             858..1055
                     /locus_tag="ACZPCG_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285021.1"
                     /translation="MTYLELIARVGTSPMDVVKMYFKGMITEKELSNVIGRNRAGIVE
                     EYKNQAQTSVLEAACTGDKKK"
     gene            1117..1199
                     /locus_tag="ACZPCG_07910"
     tRNA            1117..1199
                     /locus_tag="ACZPCG_07910"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1150..1152,aa:Leu,seq:caa)
     gene            1370..1714
                     /locus_tag="ACZPCG_07915"
     CDS             1370..1714
                     /locus_tag="ACZPCG_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR1285022.1"
                     /translation="MTCEEAERLVMPYINGSITDQELEGFLKHIDHCPECREELEIYF
                     TVDVGIRQLDEGSGTYNIKGALETALELSRQRIHSLDILRTAVYAVNTLCFWSVLLVL
                     ILQLRIWGFFGV"
     gene            1736..4483
                     /gene="polA"
                     /locus_tag="ACZPCG_07920"
     CDS             1736..4483
                     /gene="polA"
                     /locus_tag="ACZPCG_07920"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003510612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HIR1285023.1"
                     /translation="MDKLVLIDGHSILNRAFYGVPDLTNAEGLHTNAVYGFLNIMLKI
                     LEEEKADHLAVAFDLPEPTFRHKMFDAYKGTRKPMPEELREQVPLIKEALTAMEIPIL
                     TLAGYEADDILGTVAKRCQEKGAEVSVVSGDRDLLQLADSHIKIRIPKTSRGTTEIHD
                     YYPEDVKREYQVSPEEFIDVKALMGDSSDNIPGVPSIGEKTATSLIVSYGSIENAYAH
                     LEEIRPPRAQKALGEHYDLAQMSKELATICTRCPVEFSYEDAKVENLYTPQAFRFMKR
                     MGFKSILSRFSPDAAGENPLEIKEQFQVITGRKEAEAVLKKARNAAVTGMQLIFAFAV
                     LPEEEGSAGEEQLSFSFDDAGGLKPDETEESEVFAAAGMGLCLGEKEIYCIRTGKEIS
                     SKDLIREAKKICGGSHPVWVLDLKAMLPVLELEDSAPVYDAGVAGYLLNPLKDTYDYD
                     TLAKDYLSEDLPSRTDLLGKGQIGDLLVQEEEKAADCACYMAYAVWKSAGILKKRLLD
                     EEMYTLYTEIEMPLIYSLYHMEKEGIQVQREKLKEYGDKLKVGIARLEKEIYEDTGRE
                     FNINSPKQLGEILFGEMKLPGGKKTKTGYSTAADVLEKLAADHPVVQKILDYRQLAKL
                     NSTYAEGLAGFIREDGRIHGKFNQTITATGRISSTEPNLQNIPVRMELGREIRRVFVP
                     KEGCIFIDADYSQIELRILAHMSGDAGLIEAYRESRDIHAATASQVFHVPLDKVTPLQ
                     RRNAKAVNFGIVYGISAFGLSEDLSISRKEAKEYIERYFESYPGVKAFLDGLVKQGKE
                     QGYVTTLYGRRRPIPEFKSSNFMQRQFGERVAMNSPIQGSAADIMKIAMIDVDRELRK
                     RGLRSRIVLQIHDELLIEAPLEEEEEVKNLLTDRMKHAASLQVALEVEAQTGASWFDA
                     K"
     gene            4504..5112
                     /gene="coaE"
                     /locus_tag="ACZPCG_07925"
     CDS             4504..5112
                     /gene="coaE"
                     /locus_tag="ACZPCG_07925"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870775.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HIR1285024.1"
                     /translation="MAEKQTFILGLIGGVGAGKSTVLTLLKDHYGFYVIQTDLTARRL
                     LEPGEAAFEEISALFGRDILRPDGRIDRARLAEQIFKDPQKRKQVNAITHPLTWNAVL
                     TEAYSCGSERVVIETALPSKEFRDKCDEMWYLYTSEENRISRLMESRGYSLEKSMAIM
                     ESQASDEEFRSLADAVIDNNHSQECTAAQVRELLHGREGIDT"
     gene            5109..5906
                     /locus_tag="ACZPCG_07930"
     CDS             5109..5906
                     /locus_tag="ACZPCG_07930"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR1285025.1"
                     /translation="MRMVSIASGSSGNCIYIGSDQTHILVDAGISNKRIMAGLNELGL
                     AGGDMAGILITHEHSDHIRGLGVISRKYHLPIYGTRKTLAGIASCSSLGNFDRQLLKP
                     VDPDVDFMVGDLKIHPFKIDHDAADPVAYRVEHQKKCVAVATDMGHFDEYIVENLKGL
                     DAVLLESNHDVRMLETGPYPYPLKRRILGDHGHLSNENAGRLLNRILHDHLKKIFLGH
                     LSKENNYPELAYETVRLEIDEGDCPYGASDFSISVASRDRMSDIVYL"
     gene            5974..6246
                     /locus_tag="ACZPCG_07935"
     CDS             5974..6246
                     /locus_tag="ACZPCG_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HIR1285026.1"
                     /translation="MSKAIITVVGKDTVGIIAKVCTYLAEHRINILDISQTIVQGYFN
                     MMMIVDISALDSSFGQAAEELEKLGEGIGVSVKIQREEIFTKMHRI"
     gene            6279..7643
                     /locus_tag="ACZPCG_07940"
     CDS             6279..7643
                     /locus_tag="ACZPCG_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009268286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HIR1285027.1"
                     /translation="MINIYEVNETNNMIEHEKLDVRTITMGISLLDCADSDLDALNEK
                     IYTKITTLAKNLVSTGKDIERDFGIPIVNKRISVTPIALVGGAACKRPEDFVTIAKTL
                     DRAAKEVGVNFIGGYSALVSKGMTTADKNLILSIPQALAQTERICSSVNVGSTKTGLN
                     MDAVELMGRIVTETAEATKENDSLGCAKLVVFCNAPDDNPFMAGAFHGVTEADAIINV
                     GVSGPGVVKVALERVRGANFEVLCETIKKTAFKITRVGQLVAQEASRRLGIPFGIIDL
                     SLAPTPAVGDSVAEILEEIGLERVGAPGTTAALAMLNDQVKKGGVMASSYVGGLSGAF
                     IPVSEDQGMIDAVSLGALTIEKLEAMTCVCSVGLDMIAIPGDTPASTIAGIIADEAAI
                     GMVNQKTTAVRLIPVIGKKVGDTAEFGGLLGYAPIIPVNTFSCEKFVTRGGRIPAPIH
                     SFKN"
     gene            7655..8290
                     /locus_tag="ACZPCG_07945"
     CDS             7655..8290
                     /locus_tag="ACZPCG_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR1285028.1"
                     /translation="MELYLIRHGRQSSRLCNVNVDLSPEGRRQAALLGHRLADWGIEV
                     LYSSSLIRAVQTAEEINRFLRLPHQVCPELGEISFGEMEGLPDEEIAVRFREFKERQA
                     AMTEDLPYPGGECAADVIRRAGPVLERLAESGHERIAVVTHGGVIRTMAAWYLGMPLE
                     KWRLLGKDLENTSITQLKWDRGHRRFTLERFNDYAHLEACPELLRKNWKEG"
     gene            8320..9582
                     /locus_tag="ACZPCG_07950"
     CDS             8320..9582
                     /locus_tag="ACZPCG_07950"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002596515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR1285029.1"
                     /translation="MDQNERKKLEEGLELFLDEGLERLLLSHPTEPSGCSRSRIRPLL
                     LKGELVFQAEEQIGKQAFHRNLTKEEAVAYVCQMLNKNFRQAEMSGIHGTGVVLVSKK
                     GKITVKIRRKERTEAAGSEKIRPRFDGQENGLRLLEHNRKKRYILEEGIPVPFLVDLG
                     VMTAEGKVVRSRYDKYRQINRFLEFIEDILPNLDPARESVILDFGCGKSYLTFAMYYY
                     LKELRGYPIRIIGLDLKEDVICRCDTLAKSYGYDRLSFCCGDIASYEGVDRVDMVVTL
                     HACDLATDYAMEKAVGWGAKVILSVPCCQHELNRQIENPLMKPVFGYGLIKERMAALY
                     TDAIRAQVLEYRGYRTQILEFIDMEHTPKNILIRAVRQGNDAAFKKAREKTGTELRKL
                     LEFLQADPAIVRLLAPELGTGERAGTDE"
     gene            complement(9611..10897)
                     /locus_tag="ACZPCG_07955"
     CDS             complement(9611..10897)
                     /locus_tag="ACZPCG_07955"
                     /EC_number="6.3.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Met) cytidine acetate ligase"
                     /protein_id="HIR1285030.1"
                     /translation="MNITGIIAEYNPFHGGHCFHIEEAKKKTNADYCIVVMSGDFVQR
                     GEPAVFDKYLRTKMALMGGADLVIELPSVFAVSSAEDFAACGVNLLSRLGAVTHLCFG
                     SEDGNLEGIQAAAKILSDESPDFSARLREGLKLGLSWPQARNRALLSMDICSCFKEDS
                     LSRLLGSPNNLLGIEYCKAIFRCRSTIRPVTIQRKGQGYHDKTLESGQASASAIRKSL
                     LASQPEKKARSHEDCVSWEPPISLSSHIPPHILPLYSQGRILGPEDCAGLLNYRLLSL
                     FHKGEDLTAFSDVSKELADRLTGQLLQYEGWEGRIRQLKTKQYTYTRISRSLTHILLE
                     ITDAQVLKAKELGYSPYARILGFKKETGSLLSLLKKQSEIPLITKTANASQILTEEAM
                     TLFKQDLFASHVRQSMEAAKYGQTGRNEFNQPICII"
     gene            11112..>11611
                     /locus_tag="ACZPCG_07960"
     CDS             11112..>11611
                     /locus_tag="ACZPCG_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285031.1"
                     /translation="MYEEKDREQMNQDEREERKVNWTTPQKLEEGRSTDESRSRETEP
                     LQNHPSGENASWRENTPYNNQFRQDRDFSGDSRKYEKKKKTSSGKRMIRRAAGITAAA
                     VLFGTVSGGVMTGVNYAGSRLMNMTGIMADVQEQPQAATEAQETAPNTMPGSGNTTTV
                     STVTDVS"
BASE COUNT         3153 a         2491 c         3156 g         2811 t
ORIGIN      
        1 attttcaaaa acagcggtca atgcttccat agactgtgtg ctccggtatt ccaaggcagc
       61 gcttgtttcc tcggcattca gctttggaaa acgtacatct tcacttagca catagcctgc
      121 catggtatag gcgtctgtga agtcattcag tcccatatcc ttcatctggt tcagattcag
      181 tgtattggtg taaaaaatgc ggctgggatg agcgccgcct tcgatgctca gttctccggt
      241 atatacagcg gtgagacgct tgggatcggc ggatatgatt tcgcaggaaa cagaaagggt
      301 atctgcactt tcattgatct cataaacatc cagaatggaa agggcatttg cctttaaatg
      361 ttcattgatc cggctctgct tttcctcatc ctccatctgc tcaatcgccg gatattcaat
      421 ggataccttt ccggaggtat aggtcatcac ctttgcagag atggcggtta ccgattcttc
      481 aggtgccttt gatggttctg tttcaggtgc ttttgtttcc tgcggtttgt tttctgcggg
      541 agcttccgtt gatgcttctg tatgaatggt agaaagatct gcttttggcg gcgtacgtcc
      601 caggatcagt ccgattatca cacctccggc cactaccaca gtgccgattg ctccgatcat
      661 tattttcttg cttttatcca tagggtcctc ctttttcatt tgtaataaaa taattatacc
      721 tgaaactcat ggacagggtt tgaagaaaaa cttacaaatt cgtgaaggaa aggaaaaaac
      781 agtcatattt ttcatggctg atgcatatag tattagagcg tgtaaagaaa gtgggggcaa
      841 gaaggaggaa aaaggccatg acataccttg aactgatcgc cagggtcggg accagtccca
      901 tggatgtggt gaaaatgtat tttaaaggga tgattacaga aaaggagtta agcaacgtga
      961 tcggacgaaa cagggccggg atcgtggagg aatataaaaa tcaggcgcaa acatctgttc
     1021 tggaagcggc atgcacaggg gacaagaaaa aataaacaga aaagcacttg ccaaaatctg
     1081 acgtttatgg taaaataaca accgttgtga aacaatgccg gcgtgtcgga atggcagacg
     1141 atgcagactc aaaatctgtt gcgggtaacc gcgtgcgggt tcaagtcccg ccgccggcag
     1201 gatgaaaagt cttaaaagct tgaaaataca gcttttgagg cttttttgtt tttcaggaat
     1261 ggatttttgg aaatgcagca ggggactttg gttgaaaacc atattttttt attgtatgat
     1321 ataaagcatg gatttacatt cacagcggcc caaaggaggt gccatttata tgacctgtga
     1381 ggaagcggag cgtcttgtga tgccctatat caatggaagt attaccgatc aggagctgga
     1441 aggcttttta aagcatatcg atcactgccc ggagtgcagg gaggagctgg agatctattt
     1501 taccgtagat gtggggatca ggcagttaga tgagggaagc ggaacgtaca atataaaggg
     1561 cgctctggaa acagccctgg agctgtccag gcagagaatt cactcactgg atattctgcg
     1621 cacggcggtt tatgcagtga acacattatg cttctggtcg gtgcttctgg ttctgatcct
     1681 gcagctgcgc atctggggat tctttggcgt ttagaatagg aggaattgaa aaaatatgga
     1741 caaacttgta ctgatcgacg gacacagtat attaaaccgt gccttttacg gagtaccgga
     1801 tctgaccaat gcagaggggc tgcataccaa tgcggtatac ggctttttaa atattatgct
     1861 gaagatttta gaggaggaga aggcggatca tctggcagtg gcctttgacc tgccggagcc
     1921 taccttccgc cacaaaatgt ttgatgcata caaggggacc cgaaagccta tgcccgaaga
     1981 gttaagagag caggtgcctc ttataaaaga agcactgacc gccatggaaa tcccgatcct
     2041 gactctggcc ggctatgagg ccgacgatat tctgggaacc gtggcaaagc ggtgccagga
     2101 gaagggcgct gaggtatcgg ttgtttcggg agaccgggat ctcttacagc tggcagacag
     2161 ccatattaag atccgtattc ccaaaaccag ccggggaacg acagagatcc atgattatta
     2221 tccggaagat gtaaagcggg agtatcaggt ttccccggaa gaatttattg atgtaaaggc
     2281 ccttatgggc gattcctctg ataatatacc gggagttcct tctattggag aaaagacggc
     2341 gaccagtttg atcgtgtctt acggttccat tgaaaatgcc tatgcccatc tggaggagat
     2401 ccgtccgccc agagcacaga aggccctggg agaacattat gatttagcac agatgagcaa
     2461 ggagcttgcc actatctgca cccgctgtcc ggtggaattt tcttatgagg atgcaaaggt
     2521 ggagaatctg tacacccctc aggcgttccg gtttatgaag cgtatgggct ttaagtccat
     2581 tttaagccgt ttttctccgg atgcggcagg ggaaaatcct ctggaaatca aagaacagtt
     2641 tcaggttatt accggaagga aggaggcaga ggcggtctta aaaaaggccc ggaatgcagc
     2701 tgtaacagga atgcagctga tttttgcctt tgcagtgctt cctgaggaag aaggcagtgc
     2761 aggagaagaa cagctttcct tttctttcga tgatgcaggc ggccttaagc cggatgaaac
     2821 agaagaaagc gaggtttttg cagcggcagg tatggggctt tgtctgggag agaaggagat
     2881 ttactgtatc cgtaccggga aagagataag cagtaaggat ctgatccggg aggcaaaaaa
     2941 aatctgcggc ggttctcatc cggtgtgggt tctggattta aaggctatgc ttcctgtgct
     3001 ggaactggaa gattcagcac ctgtttatga tgcgggagtg gcgggctatc ttttaaatcc
     3061 cttaaaagat acctacgatt atgataccct tgcaaaggat tacctttcag aagacctgcc
     3121 ttcccggacg gatcttcttg gaaaaggcca gatcggtgat ctgctggttc aggaagagga
     3181 aaaggcggcg gactgtgcct gctatatggc ctatgcggta tggaaatccg cagggatttt
     3241 gaaaaaacgc cttttggatg aagaaatgta tacactgtac acagagattg aaatgccgct
     3301 gatttacagt ctgtatcata tggagaagga aggcatacag gttcagagag aaaaattaaa
     3361 ggaatacgga gacaagctaa aggttgggat tgcccgtctg gaaaaagaaa tatatgagga
     3421 tacgggaaga gaatttaata tcaattcccc taagcagctg ggagagattc tctttggtga
     3481 aatgaagctt ccgggtggaa aaaagacaaa gacgggctat tctacggcgg cagatgtgct
     3541 ggaaaagctg gcggcagacc atccggtggt gcagaaaatc ctggattacc gccagcttgc
     3601 caagttaaat tccacctacg cggagggtct ggccggattt atcagagagg acggccgcat
     3661 ccacggcaaa tttaaccaga ccattacagc tacaggccgc atcagcagta cagaacccaa
     3721 tcttcagaat atacccgtcc gtatggaact cggacgggag atacgccggg tatttgtgcc
     3781 aaaggaaggc tgtattttta ttgatgcgga ttattctcag atcgagcttc gtatcctggc
     3841 ccatatgtcc ggggatgcag ggctgatcga ggcttacaga gaatccaggg atatccacgc
     3901 agctacggct tcccaggtat tccatgtgcc tctggataag gtgacgcctc ttcagcggcg
     3961 caacgccaag gcggtcaact tcggcattgt ttacggcatc agtgcctttg gactcagcga
     4021 ggacttaagc atctcccgaa aggaagcaaa ggagtatatt gaacgatatt ttgaatccta
     4081 tcccggagta aaggcctttt tggacggtct ggtaaagcag ggaaaggagc agggctatgt
     4141 gactaccctt tacggacggc gcaggcccat ccctgagttt aagtcatcca atttcatgca
     4201 gcgtcagttt ggagagcgtg tggctatgaa ttcccccatc cagggttccg cggcggatat
     4261 tatgaagatt gccatgatcg atgtggatcg ggagttaagg aaaagagggc tgcgttcccg
     4321 gattgtcctg cagatccacg atgaactttt gatcgaagct ccgttagagg aagaggaaga
     4381 ggtgaaaaat cttctgacag accgcatgaa gcatgcggcc agcttacagg tcgctctgga
     4441 ggtggaagcc cagacaggag cctcctggtt tgatgccaaa tagaacattc aggaggacag
     4501 acgatggcag aaaaacagac atttatcctt ggcctgatcg gaggtgtggg ggcgggaaag
     4561 agcacggttc ttaccctttt gaaagatcac tacggttttt atgtgattca gaccgatctt
     4621 acggccagga ggcttttgga gccgggggag gcagcatttg aagagatatc tgctcttttt
     4681 ggccgggaca ttttaagacc cgacggcagg attgaccggg cccgactggc agagcagatc
     4741 tttaaggatc cacaaaaacg aaaacaggta aatgcgatca cacatccgct gacctggaac
     4801 gcggtcctta cggaggcata ttcctgcggc agtgagaggg ttgtcataga aacagccctt
     4861 ccttcaaaag aatttcgtga caagtgcgat gaaatgtggt atctttatac atcagaagaa
     4921 aaccgtatat cccgcctgat ggaaagccgg ggctattctt tggaaaagtc gatggcaatt
     4981 atggaaagcc aggcttcgga tgaagagttt cgttctctag cagatgcggt gatcgataac
     5041 aaccacagtc aggaatgtac agccgcgcag gtaagggagc tgctgcacgg acgggaagga
     5101 atagatacat gagaatggta agcatagcaa gcggaagcag cggaaactgt atttatatcg
     5161 gctccgatca gactcatatc ctggtagatg ccggaatcag caataaacgc atcatggcag
     5221 ggttaaatga gcttgggctg gcaggaggcg atatggcagg gattctgatc acccatgaac
     5281 attcggatca tatccggggg cttggggtga tttccagaaa gtatcatctg cccatttacg
     5341 gtacgcgaaa gactctggcg ggaatcgcct cctgcagcag cctgggaaat tttgacagac
     5401 agcttttaaa gcccgttgat cctgatgtgg attttatggt gggagatctt aagatacatc
     5461 cctttaaaat tgatcatgat gcagcagatc cggttgcata ccgggtagag catcagaaaa
     5521 aatgcgtagc cgtggccacg gacatgggcc attttgatga atacattgta gagaacctta
     5581 agggccttga cgcagtgctt ttagaatcta atcatgatgt ccggatgctg gagacaggcc
     5641 cctatcctta tcctttaaaa cgcaggattc tgggagatca cggccatctg tccaacgaaa
     5701 acgcaggacg tcttttaaac cggatcctcc atgaccattt aaaaaagatc tttttaggcc
     5761 atctgagcaa ggaaaacaat tatccggagc tggcttatga aacggttcgc ctggaaattg
     5821 atgagggcga ctgcccctac ggagcatctg atttttctat ctccgtagca tccagagaca
     5881 ggatgagcga tattgtttat ttataattat tcttgctatt attagcaagc ttaatatata
     5941 atatagccat tacaatgaaa gaggagaatg agcatgagca aagcaatcat tacagtagtt
     6001 ggaaaagaca cagtaggaat tattgcaaag gtatgtacct atctggcaga gcaccgcatc
     6061 aatattctgg acatttccca gaccattgta cagggatatt tcaacatgat gatgatcgtg
     6121 gacatcagtg ctctggacag ttccttcgga caggcggcag aggagcttga aaagctggga
     6181 gagggaatcg gcgtttctgt taagatccag agagaagaaa tcttcacaaa gatgcacaga
     6241 atctgataag cgcaaaaaag ggaaggaaca gacgggctat gataaatatt tatgaagtaa
     6301 atgaaacaaa caacatgatt gagcatgaaa aactggatgt ccgcacgatt accatgggta
     6361 tcagtctttt agactgtgcg gacagtgatc tggatgctct gaatgaaaaa atttacacaa
     6421 aaatcaccac tctggcaaag aatctggttt ccaccggaaa ggatattgaa cgtgattttg
     6481 ggatccccat cgtaaataag cgtatttccg ttacccctat tgcgctggtg ggcggtgcag
     6541 cctgcaaaag gccggaggat tttgtgacta ttgcaaagac tcttgaccgg gcggcaaagg
     6601 aagtgggagt aaactttatc ggcggctatt ctgcactggt aagcaagggc atgaccacag
     6661 cagataagaa tctgatcctt tctatccccc aggccctggc tcagaccgaa cggatctgca
     6721 gctctgtcaa cgtgggttct acaaagacag gcctgaatat ggacgcggta gagcttatgg
     6781 gacggattgt gacggaaacg gctgaggcaa caaaggagaa tgactcctta ggctgtgcca
     6841 agctggtggt attctgcaat gctcccgacg ataatccctt tatggcaggt gcattccacg
     6901 gcgttacaga ggctgacgcc attatcaatg taggtgtcag cggcccgggc gttgtgaagg
     6961 tggctctgga aagggtgaga ggtgctaatt ttgaggtgct ctgtgaaacc atcaaaaaga
     7021 cggcctttaa gatcacccgc gtaggccagc tggtagccca ggaagcttcc agacgtctgg
     7081 gtattccctt tggcatcatt gacctttctc tggcccctac tcctgcggta ggcgacagcg
     7141 tggcagaaat tctggaggag atcggcttag aacgggtagg cgctcctggc accacagcag
     7201 ctcttgccat gttaaacgat caggtaaaga agggcggcgt tatggcgtcc tcttatgtag
     7261 gcggtttaag cggtgcattt atcccggtca gtgaggatca gggcatgatc gatgctgtaa
     7321 gccttggcgc ccttaccatt gagaagctgg aggccatgac ctgcgtctgc tcagtaggcc
     7381 tcgatatgat cgccattccc ggtgacacac cagcttctac cattgccggc atcattgcgg
     7441 atgaggctgc cattggtatg gtaaaccaga aaacaaccgc agtacgcctg atccctgtga
     7501 tcggaaaaaa ggtaggagat acggcggaat ttggcggact tttaggatat gctccgatta
     7561 ttcccgttaa tacattttcc tgcgagaagt ttgtgacaag aggcggacgg attcctgctc
     7621 ctatccacag ctttaagaat taagaggccg gtttatggag ctgtatctta tacgtcatgg
     7681 ccgccagagc agcaggctct gtaatgtaaa tgtggatctt tccccggagg gccgccgtca
     7741 ggctgccctt cttggccata ggcttgcaga ctggggaatt gaggttcttt attccagcag
     7801 cctgatccgg gcggtacaga cggctgagga gataaaccgt tttttacgcc tgcctcatca
     7861 ggtctgtccg gagcttggag agatttcttt tggggaaatg gaggggcttc cagatgaaga
     7921 gatcgcagtc cgcttccggg aatttaaaga gcgtcaggct gccatgacag aggatctgcc
     7981 ctatccgggc ggagaatgtg cggcagatgt gatccgcagg gcaggcccgg ttttggaacg
     8041 gcttgcagag agtggtcatg agaggattgc cgtggtgact cacggcggag tgatccgaac
     8101 catggcagcc tggtatctgg ggatgcctct ggaaaaatgg aggcttcttg gaaaggatct
     8161 ggaaaataca agtatcacac agttaaaatg ggacagaggc cacaggcgtt ttacactgga
     8221 acgctttaat gactatgccc atctggaggc ctgtccggag cttttgagaa aaaactggaa
     8281 ggaaggctga aacagactga aataaaaagg agtgttccca tggatcagaa tgaacgcaaa
     8341 aagctggaag aagggctgga gctgttttta gatgaggggc tggagcggct gcttttaagc
     8401 catcccacag agccttccgg gtgcagccgc agccggatcc gtcccctgct tttaaagggg
     8461 gagctggtat ttcaggcaga ggaacagata ggaaaacagg cctttcaccg aaacctgacg
     8521 aaagaagagg cggtcgccta tgtctgccag atgcttaata agaatttcag acaggcagag
     8581 atgagcggta ttcatggaac cggagtggtt ttggtaagca aaaagggaaa aataaccgta
     8641 aaaatccgaa ggaaggaacg gacagaggct gccggttccg aaaaaatccg tccgcgcttt
     8701 gacggacagg aaaacgggct gcgccttttg gagcacaacc gcaaaaagcg ttatattctg
     8761 gaagagggaa tcccggttcc ttttttagtg gatctggggg ttatgacggc agaaggaaag
     8821 gttgtccgca gccgttatga taaataccgc cagatcaatc ggttcctgga atttatcgaa
     8881 gatattctcc cgaatctgga tcctgcgaga gagtctgtga tcctggattt tggctgcgga
     8941 aagtcctatc tgacctttgc tatgtattat tatctgaagg agctaagggg gtatccgatc
     9001 cggatcatcg gccttgactt aaaggaagat gtgatctgcc gctgtgatac tctggcaaaa
     9061 tcctatggtt atgaccgctt aagcttctgc tgcggggata ttgcttccta cgaaggcgta
     9121 gatcgggtgg atatggttgt aacccttcat gcctgtgatc tggctacgga ctacgccatg
     9181 gaaaaggctg tgggatgggg agcaaaggtg attttgtctg ttccctgctg ccagcatgag
     9241 ttaaaccgcc agattgaaaa tccgttaatg aagccggtat ttggctatgg cctgatcaaa
     9301 gagcgtatgg ctgccctgta tacggatgcc atccgggctc aggtactgga gtaccggggc
     9361 tatcgtaccc agattttgga atttatcgac atggaacaca ctcccaaaaa catcctcatt
     9421 cgggcagtcc gccagggaaa tgacgctgct ttcaaaaagg cacgggagaa gaccggcaca
     9481 gaacttagaa agcttctgga atttctccag gcagatccgg cgatcgtacg gcttcttgcg
     9541 ccggaacttg gaacaggaga acgtgcagga acagatgaat gatgccggaa agaagggcag
     9601 gactgaccgg ctatataatg cagatgggct gattaaattc gtttcgcccg gtctgtccat
     9661 actttgctgc ttccatactc tggcgtacat gggaggcaaa cagatcctgc ttaaaaagcg
     9721 tcatggcttc ctcagtaagg atttgtgagg catttgcagt ttttgtaata agcgggatct
     9781 cagactgttt ctttaaaaga gacaaaagag atcctgtctc ttttttaaag cccaggatcc
     9841 tggcataggg ggaatagccc agttccttgg cctttaatac ctgcgcatct gtgatttcca
     9901 gaagaatatg ggtcagagag cggctgatcc gcgtataggt atactgcttt gtttttaact
     9961 ggcggatcct tccttcccag ccctcatact gcagcagctg tccggtgagc cggtcagcca
    10021 gttccttaga tacatctgaa aacgcagtca gatcctcccc cttatggaac agggaaagaa
    10081 ggcggtagtt tagaagccct gcgcagtctt caggcccaag aatccttcct tgggaataaa
    10141 gcgggagaat gtggggagga atgtgggacg ataaagaaat gggaggctcc caggaaacac
    10201 aatcttcgtg agaccgggct tttttttccg gctgagatgc aagaaggctt tttcggatcg
    10261 cagatgcgga tgcctgtccc gattccagcg ttttatcgtg atatccctgt ccctttctct
    10321 ggatggttac aggacggatg gtgctgcggc aacggaaaat ggctttgcag tattcaattc
    10381 ccagaagatt gtttggagat cccagaaggc gacttaatga atcctccttg aaacaggaac
    10441 atatgtccat ggaaagaagg gctctgtttc ttgcctgggg ccagcttaag cccagcttta
    10501 atccttcccg cagacgggct gaaaaatcag ggctttcatc ggataaaatc tttgcagcgg
    10561 cctggattcc ttccagattt ccatcctcac ttccgaagca gagatgggtt acggctccca
    10621 gccggcttaa aagatttacc ccgcaggcgg caaagtcttc ggcgctgctg accgcaaaga
    10681 cggagggcag ctcaatcaca agatctgccc ctcccatcag agccattttt gttcggaggt
    10741 atttatcaaa aacggccggt tctccccgct ggacaaaatc cccgctcata acgacaatgc
    10801 agtaatcggc atttgtcttt ttttttgctt cttctatatg aaaacagtgt cctccgtgaa
    10861 aagggttata ttctgcaata attccggtta tattcataag ctgctccttt agcggcagaa
    10921 ctgccgtatt ctcactatta tattacctgt ttttgcaaaa ttatcaaccg gaaacttaaa
    10981 acggcctgca gtttaacagg caggaacata aacacaagga cacaaaattt ccacaatttc
    11041 gtcacaaatc aaacaattct ctttcgtatg attgaaccat caaaaggaag tgtacgaaag
    11101 gagcattcgt tatgtacgaa gaaaaagata gagagcagat gaatcaggat gaaagagagg
    11161 aaagaaaggt aaactggact acgccccaaa agctggagga gggacgcagc acagatgagt
    11221 cccggagcag agaaacagaa ccgctgcaga accatccttc cggtgaaaat gcatcctgga
    11281 gggaaaatac cccatacaat aaccagttcc gacaggacag ggatttttcc ggggacagcc
    11341 ggaaatatga aaaaaagaaa aaaacatctt ctggaaagcg catgatacgt agggcagcag
    11401 ggattactgc ggcagctgtc ctgtttggca cggtatccgg cggtgtgatg acaggcgtta
    11461 actatgcagg ttccagactg atgaatatga cagggatcat ggctgatgta caggaacagc
    11521 cgcaggctgc aacagaggct caggaaacgg cgccaaatac tatgccgggc agcggaaaca
    11581 caaccacagt ttccactgta acggatgttt c
//
LOCUS       DBMDUH010000080        11422 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_2856_length_11422_cov_6.892147, whole genome shotgun sequence.
ACCESSION   DBMDUH010000080 DBMDUH010000000
VERSION     DBMDUH010000080.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 11422)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11422)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11422
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2856_length_11422_cov_6.892147"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(8..1174)
                     /locus_tag="ACZPCG_07965"
     CDS             complement(8..1174)
                     /locus_tag="ACZPCG_07965"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR1285032.1"
                     /translation="MVKYSKNTLSSRSGKVLINSGFFFWNVCDKIYIKNSKAVFGEGY
                     MKSSFWKKLDTYIDELCRETISGCSCLILQNGIEQYRKYAGKCNLQTGEAPNENTLYR
                     IYSLTKLYTAVAALQLIEQKKLRLDAAVADYIPEYKYLNVNHVAANGWNNIEKTRSPL
                     LISHLLTMRSGISYSYDNDGPTMCQTKREVINFRQQKKDYTTQEYARLFSRIPLAFEP
                     GTHFLYGAGYDVLAAIIEIVSEMSLGDYLQRYIWEPLELENTMFRFRSIRDKENLCQM
                     YQRMENGDLIPVADKDWFYQPGRKFEEGGGGVLSTLDDYMRFQQCLLDGGIWRKHRIL
                     SEGYVEMMHTNVLTQEQMRDFTIPDYGYGYGVRVRVREEGKTPVELEFNQVEKV"
     gene            1269..2300
                     /locus_tag="ACZPCG_07970"
     CDS             1269..2300
                     /locus_tag="ACZPCG_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018594978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR1285033.1"
                     /translation="MRKRFCAAALALTAALSAGLLTGCSSASGGETMIVRIGHNQSTN
                     HPTHIALAAFEEFIESRLGDKYDVEVFPSELLGSQNEMVQLTQTGAINFCVASNSLLE
                     TFSQNYTLFNLPYLFSSSEAYHASMDDTSITEPIFESTRQAGFEAVTWLDAGTRNFYT
                     VNKPIHSPADLKGLKIRVQQSPTNVQMMNLLGGSATPMGFGDVYTALQSKIIDGAENN
                     ELALTDNGHGDVCKYYSYDMHQMIPDILIGNIAFLDSLSPEERAIFDEGFDLVNQIQR
                     EEWTIAVDAAKKRASGDQKVNFLYPDTAPFKEAVIPMHESMLGQHPDFVPIYDAIQAY
                     NQKYPSAES"
     gene            2316..2813
                     /locus_tag="ACZPCG_07975"
     CDS             2316..2813
                     /locus_tag="ACZPCG_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIR1285034.1"
                     /translation="MKKLRNVLTSLLNILAGASLIAMVALTCWQVFTRYILQNPSPWS
                     EELVAYLFAWASLLGASIITGERGHMNIPIVTDHFTPGIQKLLAIFGELIAFAFSLII
                     LVYGGIKITSLAMGQMTSALGVAVGVFYVVMPLCGILNMIYTVLNIVDISKNGVITAQ
                     EEKEA"
     gene            2818..4119
                     /locus_tag="ACZPCG_07980"
     CDS             2818..4119
                     /locus_tag="ACZPCG_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004064822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR1285035.1"
                     /translation="MAMESLLIIIVVLAVLLIVGVPISYSIGISALIAILRTVPLEVS
                     VVTGAQRIFVGMSKFSLTAIPFFILAGNLMNQGGIAKRLVDFVMAILGKLPGALLVTN
                     VGANALFGAISGSASAAAAAVGSMVEKGEEEQGYDKALCAATNGASAPSGLLIPPSNA
                     LITYSLAAGGTSVAALFMAGYIPGIIWAVACMAVAVIISKKKGYKGIPGKYDWKNLWV
                     CTLRALPSLSLIVVVIGGIIGGVFTATEGSAIAVIYALILGILYRNITLKSFWKIAVD
                     SAKMSGMVVFLIGVSNILGWVMAFTQIPQAISDALLGLTSNPIIILLIMNVILLIAGT
                     FMDVTPAILIFTPLFLPVVKTFGMDPVQFGLILVYNLCIGNITPPVGNTLFVAIKVGR
                     SSLSRVMPYMLWYYVAILVGLLMVTYIPAVSLGLPAMAGLI"
     gene            complement(4242..5315)
                     /gene="prfA"
                     /locus_tag="ACZPCG_07985"
     CDS             complement(4242..5315)
                     /gene="prfA"
                     /locus_tag="ACZPCG_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003502720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HIR1285036.1"
                     /translation="MFDKLDDILIHYEELMLMLGDPDVTQDTKRFTRLMKEQADLAPI
                     VEAYKQYKQAKQDVEDSLAMLEEESDEDLREMAKEELSDAKKRIEELEHELKILLLPK
                     DPNDEKNIILEIRAGAGGDEAALFAAELYRMYSNYADSQRWKVEIVSLNENGIGGFKE
                     VVAMVTGKGAYSKLKYESGVHRVQRVPETESGGRIHTSTATVAVMPEAEDVDVQIDMN
                     DCRIDVMRASGNGGQCVNTTDSAVRLTHIPTGIVIYSQTEKSQLQNKEKAFRLLRSKL
                     YDIELEKKQNAEAAERRSQIGTGDRSEKIRTYNFPQGRVTDHRINLTLYKLDKILNGD
                     IQEILDACIAADQAAKLAKMNEN"
     gene            complement(5335..6252)
                     /gene="prmC"
                     /locus_tag="ACZPCG_07990"
     CDS             complement(5335..6252)
                     /gene="prmC"
                     /locus_tag="ACZPCG_07990"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015573457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HIR1285037.1"
                     /translation="MSAERVWEETTLGQLYESGRKKLEESGVPDAALDARLLLLEAFD
                     LNFASFLTRRDQPLFKQAIGGEEEIYRLKQAAEQYEQAVLARAERIPLQHLTGCQCFM
                     GLEFKVNEWVLIPRQDTETLVERVLEENRDKNSAILDVCTGSGCIAISLVKLGEYRSV
                     TALDISKEALKVAEANRERILGEEKERLQLIKSDMFEGLEPKAQFDVIVSNPPYIPSE
                     VIEGLEPEVRDHEPRLALDGEADGLKFYRILAERSMAYLKPGGKIYMEIGFDQGKDVS
                     ELFEQAGFEGLKVIKDMAGLNRVVQAKRP"
     gene            complement(6249..7196)
                     /locus_tag="ACZPCG_07995"
     CDS             complement(6249..7196)
                     /locus_tag="ACZPCG_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HIR1285038.1"
                     /translation="MKYSGIGGQAVIEGIMMQNGDDYAIAVRKPDGEIEVKRDRYVSL
                     TEKYPILGLPFIRGVFKFADSMVVGMRALTWSCSFFEDDEDAQPGKFEQWLDRVFGEK
                     LESILMTVVMIFSFIMAIGIFMLLPLGIARICKPFIPSETAMAVLEGVIRIVIFIIYI
                     KLVSRMEDIRRTFMYHGSEHKCINCLEHGLALNVANVRGSSKEHKRCGTSFIMIVMII
                     SILFFMVIRTDTVWLRFLSRIVLIPVIAGVSYEFLRFAGRHDSKLVNLLSRPGMWMQG
                     LTTTEPDDSMIEVAIAAVEEVFDWKAYLDENFPGWKETE"
     gene            complement(7308..7514)
                     /gene="rpmE"
                     /locus_tag="ACZPCG_08000"
     CDS             complement(7308..7514)
                     /gene="rpmE"
                     /locus_tag="ACZPCG_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HIR1285039.1"
                     /translation="MREGIHPNYYQAKVVCNCGNEFVTGSTKQDIHVEVCSKCHSFYT
                     GQQKAAAARGRIDKFNRKYGINAQ"
     gene            complement(7802..8860)
                     /locus_tag="ACZPCG_08005"
     CDS             complement(7802..8860)
                     /locus_tag="ACZPCG_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotS"
                     /protein_id="HIR1285040.1"
                     /translation="MIDRYLEALEQYEMEIISVRKGRGAWICETDQGLKLLREYRGTV
                     RRLEIEDQILGMIDTRTSLRTDRYERNREGGLLTMAGDGTRYILKEWFSDRECNIRDS
                     YEVRQSIARLAMLHAQLARIPFQEEWRMGSVCREQAGPEMNRHIREMQKARSFIRRKR
                     GKTEFELCVIGNYNGFYEQAKEAADEMMRLWGRNRAAAGQHIAEEDGQLMAAELSAES
                     VSGLSLCHGDLDQHHVLMGRGYTAIVEYNRMHLGIQASDLYRLMRKALEKHGWDTELG
                     LSMLDSYQRVRPMDRRERKSLACMFLFPEKYWKQLNFYYNTNKAWIPAKSTDKLKSLE
                     DQEPARRRFIRRLMAECG"
     gene            9059..10387
                     /locus_tag="ACZPCG_08010"
     CDS             9059..10387
                     /locus_tag="ACZPCG_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015615277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HIR1285041.1"
                     /translation="MKKFSVVIAGGGSTYTPEIILMLLDNLDKLPLRSIKLYDNDEER
                     QNRLAKACEIIVKEKDPEIEFLATSDPEAAYTDVDFCLAHIRVGQLPMREKDEQIALK
                     YGVVGQETCGPGGIAYGMRSIGGVIENIDYMEKYSPNCWMLNYSNPAAIVAEACRRLR
                     PHSRVINICDMPIGIENSIAKILGFESRKDMTIRYYGLNHFGWYTSIKDKDGNEYIDK
                     LLEHELKYGNVAAGENASDFTDNSWLETARKVKDIVTLDPTHSPNSYLQYYLFPDDMV
                     AHTDPEYTRARQVIDTREKRVFGECARIAQTGTSEGTSLQIGIHASFIVDLATALAYN
                     TGERMLLIVENNGAIENFSPDAMVEIPCIVGKDGYEPLSVGKIPTFQKGLMEEQVAVE
                     KLTVDAWIEGSYHKLWQALTLSKTVPSANVAKKLLDEYIEANKGYWPDLK"
     gene            10402..10722
                     /locus_tag="ACZPCG_08015"
     CDS             10402..10722
                     /locus_tag="ACZPCG_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HIR1285042.1"
                     /translation="MVNIEKMAETAMYIVTYAGTAKSCYMNGLSEAKKGNFEKARELL
                     KEGDNNMAQAHQSHIGVLEEEVTSLTPQISLLMCHAEDQIMGAETLKTVIIELIELYQ
                     KKEN"
     gene            10725..11342
                     /locus_tag="ACZPCG_08020"
     CDS             10725..11342
                     /locus_tag="ACZPCG_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006775288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285043.1"
                     /translation="MEIQPQDYNIWAGSQTRNGFAADEIISSLQKAIRRAMEEQACQY
                     AYELYVSGPIFLEKLWRRLLTISVEDIGFGNLNAAVYVNTMNEMRKNFPYDDGDQPMY
                     FIHAIRILCSSNKDRSSDFLKNIIIKEAAMGKKPEIMDLALDKHTVRGKAMGRGSKHF
                     FEEACKVFPQIPVDNDYRERYGKILEEYDPSKNVPGAFKYNPAQA"
BASE COUNT         2883 a         3015 c         2425 g         3099 t
ORIGIN      
        1 aaaatgtcta aactttttcc acctggttga attcaagttc taccggtgtc tttccctcct
       61 ctctcactct cactctcacc ccatatccat atccataatc tggtattgta aaatccctca
      121 tttgctcttg tgtaagcaca tttgtatgca tcatttctac atatccctct gacaaaatcc
      181 tatgttttct ccatattcct ccatcaagca aacattgttg aaatctcata taatcatcca
      241 gtgtagacag cacccctcct ccgccttctt caaactttcg acccggctga taaaaccaat
      301 ctttatctgc aaccggtatt aaatcgccat tttccatacg ttgatacatc tgacacaaat
      361 tttccttatc ccttatgctt ctgaaacgaa acatggtatt ttccaactcc aatggctccc
      421 atatataacg ctgcaagtaa tcacccaggc tcatttctga cacaatttct ataattgctg
      481 caaggacgtc ataccctgct ccatataaaa aatgagttcc cggttcaaag gctaaaggaa
      541 ttctggaaaa tagtcgtgca tattcctgag tggtataatc ctttttctgt tggcggaaat
      601 tgatcacttc tcttttcgtc tgacacatag tcggaccgtc attgtcataa gaataggata
      661 tacccgaacg catagttaaa aggtgagaaa tcagcaaagg gcttctggtt ttttcaatat
      721 tattccatcc atttgccgca acatggttaa cattaagata tttatattct ggaatatagt
      781 cagcaactgc tgcatctagc cgtaattttt tctgctctat cagttgtaga gcagctacag
      841 ctgtataaag tttcgtaaga gaatatatac gataaagtgt attctcattc ggtgcttccc
      901 cagtttgcaa attgcatttt cctgcatatt ttctatactg ttctatgcca ttttgaagta
      961 tcaaacatga acatcccgag atagtttccc tgcataattc atctatataa gtatctaatt
     1021 tcttccaaaa actgcttttc atatatccct ctccaaaaac tgctttagaa tttttaatat
     1081 aaattttatc acaaacattc caaaagaaaa agccactatt aattaatacc ttaccacttc
     1141 tggaacttaa tgtattttta ctatatttta ccacattttc ccagtatttt tctggaagcc
     1201 cctgtccata attgatataa taggagtgat gaagattctc aaaatacaat acagaaagga
     1261 agatttttat gaggaagaga ttttgcgcag cagcgctggc actgacagcc gcgctatctg
     1321 ccggactgct taccggctgc agcagtgcct ccggcggcga aacaatgatc gtccggatcg
     1381 gtcacaacca gtccacgaac catcccaccc acatagcatt ggctgctttt gaagaattta
     1441 tcgaaagcag gctgggagac aaatatgatg tggaagtatt tccaagcgag cttttaggtt
     1501 ctcagaacga aatggttcag ctgacgcaga caggcgccat caatttctgc gtggcttcca
     1561 actctcttct ggaaaccttc agccagaact acactctgtt taatcttccc tatctgtttt
     1621 cttccagcga agcctaccat gcgtccatgg atgatacttc catcacggaa cccatctttg
     1681 aatcaaccag acaagctggt tttgaggctg ttacctggct ggatgcaggc acccggaatt
     1741 tctatacggt aaataagccc atccattctc ctgctgattt aaagggactt aagatccgcg
     1801 tccagcagtc ccctaccaac gtacagatga tgaacctttt aggaggctct gccactccca
     1861 tgggctttgg agatgtgtac acggccctcc agtccaagat catcgacgga gcagaaaata
     1921 atgaactggc tctgacagac aacggtcatg gagatgtatg caaatactat tcctacgata
     1981 tgcaccaaat gatccctgat atcctgatcg gaaatattgc ttttctggac agtctgtccc
     2041 cggaagaacg agccattttt gatgagggct ttgacctcgt gaatcagatc cagagagagg
     2101 aatggaccat cgcagtggat gcggctaaaa aacgggcttc cggagaccag aaggtgaatt
     2161 tcctctaccc ggatacagcc ccatttaagg aagcggttat tcccatgcat gaatccatgc
     2221 tgggccagca cccggatttt gttcccatct atgatgcgat acaggcttac aaccagaagt
     2281 atccgtcagc tgaatcctaa aaggaggtga ccggcatgaa aaagctgaga aatgtactta
     2341 catccctttt aaatatactg gcaggcgcca gcctgatcgc tatggttgcg ctcacctgct
     2401 ggcaggtatt tacccgatac atcttacaga atccgtcccc gtggtctgag gagcttgtgg
     2461 cttacctctt tgcatgggcc agccttttag gcgcttccat catcacagga gaacggggac
     2521 atatgaatat tcccattgtc acagaccatt tcactcccgg catccagaag ctcctcgcaa
     2581 tctttggaga gcttatcgca tttgcctttt ccctgatcat cctggtatac ggcggcatca
     2641 agatcacaag cctcgccatg ggacagatga cctctgccct gggcgttgct gtaggtgtgt
     2701 tttatgtggt aatgccttta tgcggtattt taaacatgat ctatacagtt ctcaacatcg
     2761 tggatatctc aaaaaacggc gtcattacgg cgcaggaaga aaaggaggca tagacttatg
     2821 gcaatggaat ctctgcttat cataattgtt gttctggccg tgctcctgat cgtcggcgta
     2881 ccgatttcct attccatcgg catttccgcc ctgatcgcca tcctgcggac cgtgcctttg
     2941 gaagtatcgg tggtaaccgg cgctcagcgt atctttgtgg gcatgagcaa attcagcctg
     3001 acggccattc ccttttttat cctggcagga aacctgatga accagggcgg catcgccaaa
     3061 cggctggttg attttgtgat ggcaatcctt ggcaagctgc ccggtgccct tttagtgaca
     3121 aacgtagggg caaacgccct tttcggcgct atttccggtt cggcctctgc cgccgcagca
     3181 gccgtaggaa gtatggtgga aaagggcgaa gaggaacagg gctatgacaa ggcgctctgt
     3241 gctgccacca atggagcttc tgctccctca ggcctcctaa tcccgccctc caatgccctg
     3301 atcacctatt ctctggcagc cggaggcacc tctgtggcag cactctttat ggccggttat
     3361 attccgggca tcatctgggc ggtggcctgt atggcagttg ctgtgatcat ttcaaagaaa
     3421 aaaggctata agggaatccc tggaaaatac gactggaaaa acctgtgggt ctgcaccctc
     3481 agagccctgc cctccctttc cctgatcgta gtggtaatcg gcggcatcat cggcggtgtg
     3541 tttacagcca cagaaggctc tgccattgcg gtaatctacg ccctgatcct cggcatcctc
     3601 taccgcaaca ttactttaaa atccttctgg aaaatcgcag tagacagcgc caagatgagc
     3661 ggtatggttg tattcctgat cggcgtttcc aatatccttg gctgggtcat ggcatttacc
     3721 cagatccctc aggccatctc cgacgccctc ttaggcctta caagcaatcc cattatcatc
     3781 ctgctgatca tgaatgtcat ccttttaatc gcaggaacct ttatggacgt aactccggct
     3841 atcctgatct tcactcccct gtttcttccg gtggttaaga cctttggcat ggatccggtt
     3901 cagttcggcc tgatcctggt atacaacctc tgtatcggaa atattactcc gccggtggga
     3961 aataccctgt ttgtagccat caaggtcggg cgaagttccc tttcccgcgt catgccctat
     4021 atgctctggt actatgtggc aattcttgta gggcttctca tggtgaccta tatccctgcg
     4081 gtgagcctgg ggctccctgc aatggccgga ctgatctgac cgatattttt tcttccataa
     4141 cataaaagac ggctggcgca gctatgtggt gatctccaca cagctctgcg ccagcccttt
     4201 cgtattcgtt cgttccgtat tctttattta gttgtaacta ttcagttctc attcatctta
     4261 gccagctttg ctgcctgatc tgcggcaatg caggcatcca ggatctcctg gatatctccg
     4321 tttaaaatct tatccagctt ataaagggtc agattgatcc ggtgatccgt cacacggccc
     4381 tgtgggaaat tgtaggtacg gattttctcc gaacggtcgc cggtaccgat ctggctgcgg
     4441 cgctctgctg cctctgcatt ctgctttttc tcaagctcaa tatcatagag cttggagcgc
     4501 aggaggcgga aggccttttc cttattctga agctgggatt tctccgtctg gctgtagatt
     4561 acgattcctg tgggaatatg ggtaagacgc acggcagagt ctgtggtatt tacacactgc
     4621 ccgccgtttc cggaggcacg cattacgtcg atgcggcagt cattcatatc gatctgtacg
     4681 tctacatcct cagcctcagg catcacggct accgtcgctg tggaagtatg gattcgtccg
     4741 ccggactctg tctcaggcac acgctgtacc ctgtgcacgc cgctctcgta tttcagcttg
     4801 gagtatgcgc cctttcccgt aaccatggct accacttcct taaagccgcc gatcccgttt
     4861 tcatttaagc ttacgatctc caccttccag cgctggctgt ccgcataatt ggaatacatc
     4921 cggtacaact cggcagcaaa caaggctgcc tcgtctccgc ctgcacctgc acggatctcc
     4981 agaatgatgt tcttttcatc atttggatcc ttgggaagaa ggaggatctt caattcatgc
     5041 tccagctcct cgatgcgctt ctttgcgtca gacagttctt ccttcgccat ttcccgaaga
     5101 tcttcatcac tctcctcttc cagcattgca aggctgtctt ccacatcctg tttcgcctgc
     5161 ttatactgct tatatgcctc tacgatggga gccagatccg cctgctcctt catcagtctg
     5221 gtaaaacgct ttgtatcctg agtcacatca ggatccccca gcatcagcat cagttcttcg
     5281 taatggatca gtatatcatc cagtttatca aacataatct tctctctcct atcatcacgg
     5341 ccttttagcc tgcaccaccc ggttcagccc ggccatatcc tttatcacct taaggccctc
     5401 aaaaccggcc tgttcaaaaa gctctgaaac gtccttcccc tggtcaaagc ctatctccat
     5461 atagatcttc cctccgggct ttaaatatgc catggaacgt tctgccagaa tccggtaaaa
     5521 tttcagcccg tccgcttccc cgtccagagc cagccgcggc tcatgatccc gcacctcagg
     5581 ttccagtcct tctatgacct ctgaggggat atagggcgga ttggacacaa taacgtcaaa
     5641 ctgcgctttg ggttccagcc cttcaaacat atcgcttttt atcagctgga gccgctcctt
     5701 ttcttctccc aggatccttt ccctgttagc ctctgccacc ttgagcgctt ccttagagat
     5761 atcaagagcg gtcactgagc ggtattcccc cagttttaca aggcttatag caatacagcc
     5821 ggagccggtg cacacatcca gtatggcgct gtttttatct ctgttttcct ccaaaacacg
     5881 ctccaccagt gtttccgtat cctgtctggg aataagcacc cattcattta ccttaaattc
     5941 cagtcccata aagcactggc agccagtcaa atgttgcagg ggaatcctct ctgctcttgc
     6001 aagaaccgcc tgttcatact gttctgccgc ctgttttagc ctgtaaattt cctcttctcc
     6061 accaatggcc tgtttaaaaa gaggctggtc tcttctggtg agaaaagagg caaaattcag
     6121 atcaaacgcc tccagcagca aaagtctggc atccagcgca gcgtccggca ctccggactc
     6181 ttccagtttt ttcctcccgg actcataaag ctgtcctaag gtcgtctctt cccatactct
     6241 ctccgcactc attctgtttc cttccagcct gggaagtttt catccaggta cgctttccag
     6301 tcaaaaacct cttccactgc cgcgatcgcc acttcgatca tggagtcatc cggctctgtg
     6361 gtggtcagac cctgcatcca catgccggga cggctcaaaa ggtttacaag cttactgtca
     6421 tggcggcctg caaatcggag aaactcatag gacacgcctg cgatcaccgg tatcagcacg
     6481 atccggctta aaaagcgcag ccagactgta tccgtacgaa tcaccataaa aaacaggata
     6541 cttatgatca ttacaatcat gataaaactg gttccgcagc gcttgtgctc cttggagctg
     6601 ccccggacat tggctacatt taaagccagt ccatgctcaa gacagttgat gcatttatgc
     6661 tccgaaccgt ggtacataaa ggtacggcgg atatcctcca tacgggatac cagtttaata
     6721 tagataataa aaatcacaat gcggatcacg ccctcaagaa ccgccatcgc cgtttccgaa
     6781 gggataaaag gcttacagat acgggcgatc ccaaggggaa gcagcatgaa aatgccgatt
     6841 gccataataa aagaaaaaat catgacaacc gtcataagaa tactttccag cttttcaccg
     6901 aatacccggt ccagccactg ctcaaacttt cccggctgag catcctcatc atcctcaaaa
     6961 aagctgcagg accaggttaa agcacgcatc cccaccacca tagagtctgc aaatttgaaa
     7021 acgccgcgga taaaaggaag gcccaggatg gggtatttct ccgtaagact cacataccga
     7081 tctcttttta cctcaatctc tccgtcaggc tttcggactg caatggcgta atcgtcaccg
     7141 ttctgcatca tgatgccctc gatcaccgcc tgaccgccga ttccagaata tttcattgta
     7201 tcatttcctc tccctgccgc agcaagctgc ggctgtgttt tgccaaaaga acacaaaagg
     7261 gctgagcctg atctctcagg ctcaacccct tgattttttc tcgcttatta ctgggcgtta
     7321 atgccatact tacgattgaa cttgtcaata cgtccacggg cagcagcagc cttctgctgg
     7381 ccagtataga aagaatggca cttggagcaa acctcaacgt ggatgtcctg ctttgtggag
     7441 cctgttacga actcgttgcc acagttgcaa actacctttg cctggtagta atttggatgg
     7501 attccttctc tcattttcat tcacctctct gattatttgc ggtttccgac cgcggccttg
     7561 tatgccaatc tgcctctgta cactgcaggc agccgaccgg ggccgtcacc agaacgcaca
     7621 gatatcagca tcttttaagt tatacacgcc cttaccgcaa gttcctgact cacgcaggcg
     7681 ctaaaactgc tctccatatt caggacagct taatcagtat agcatagaat tttttgtatt
     7741 gcaagaggga atgtggctga gtttttgcat ttttcccggc tttccacacc attttcagga
     7801 cttacccgca ctctgccatc agtcggcgga taaaccggcg gcgggccggc tcctgatcct
     7861 ccaggctttt gagcttatcc gtgcttttag ccggaatcca ggccttgttt gtattgtaat
     7921 agaaattcag ctgcttccag tatttttccg gaaagaggaa catacaggcc agactctttc
     7981 gctccctccg gtccataggg cgcacccgct ggtaggaatc cagcatggac aggccaagct
     8041 ctgtatccca gccatgcttt tccagggctt ttctcatcaa gcggtaaaga tctgatgcct
     8101 ggattcccag atgcatccgg ttatactcca caatggccgt atatccccgc cccatgagca
     8161 catgatgctg gtctaagtcc ccatggcaga gagaaaggcc tgaaacagac tctgcggaca
     8221 gctctgctgc catcaactgt ccatcttcct ctgctatatg ttgtccggca gcagctctgt
     8281 ttcttcccca aagcctcatc atctcatccg ccgcttcttt tgcctgctca taaaagccat
     8341 tataatttcc aatgacgcaa agctcaaatt ccgtctttcc ccgttttctg cggataaagc
     8401 ttctggcctt ctgcatttcc ctgatatggc ggttcatctc cggacctgcc tgttccctgc
     8461 agaccgaacc catgcgccat tcctcctgaa agggaatccg ggccagctgg gcatggagca
     8521 tggcaaggcg ggcaatggac tgacggacct catagctgtc tcttatatta cattcccggt
     8581 cggaaaacca ctcctttaat atgtagcgtg ttccgtctcc cgccatagtc aatagcccgc
     8641 cttcccggtt tctctcgtac cggtctgtcc gtaggcttgt acgggtatct atcattccca
     8701 gaatctggtc ttctatttcc agtcttctca cagtccccct gtattcccgt aaaagcttaa
     8761 gcccctgatc cgtctcgcag atccaggccc cccttccctt tctcacagat atgatttcca
     8821 tttcatattg ttccagcgct tccaaatatc tgtcgatcat cctacccctc catctgtgaa
     8881 gtctgccccc tggcagacgc tattctaagc ttcaaacaca ttctaacgta tgtaatgcca
     8941 gatgggatta ttacatattt aaaatcttct tcctaataaa gaggaaaaaa tggatttgtc
     9001 gaaaaaatcc aaacacgcta tactgacttc agatgttacg agaaaggagc attcagcaat
     9061 gaaaaaattt tcagtagtaa tcgccggagg aggaagcacc tatacacctg agattatcct
     9121 tatgcttttg gataatctgg acaagcttcc ccttcgctca atcaagcttt atgacaatga
     9181 tgaagaacgc cagaatcgtc tggcaaaagc ctgtgaaatc attgtaaaag agaaggatcc
     9241 ggaaattgaa tttctggcca cttctgaccc ggaagccgcc tatacagatg tagacttctg
     9301 tctggcccat atccgtgtag gacagcttcc aatgagagaa aaggacgaac agatcgcttt
     9361 aaaatacggc gtcgtaggcc aggaaacctg tggccctggc ggtattgcct atggtatgcg
     9421 ttccatcgga ggcgttattg agaatattga ctatatggag aagtactctc ctaactgctg
     9481 gatgctcaat tactctaacc ccgccgccat tgtagcggaa gcatgcagac ggcttcgtcc
     9541 ccactccaga gtcatcaata tctgcgatat gcctatcggc attgaaaaca gcattgcaaa
     9601 gattcttggc tttgaaagcc gtaaggatat gacaatccgg tattatgggc tgaaccattt
     9661 cggctggtac acttccatta aggataagga tggaaatgaa tatatcgaca agcttctgga
     9721 gcatgaatta aagtacggaa atgtggcggc aggtgaaaat gcctcagatt ttacggataa
     9781 cagctggctt gaaacagcgc gcaaggtcaa ggacattgta actctcgatc caacccattc
     9841 tcccaattcc tatcttcagt actatctctt tccagatgat atggtagcac atacagatcc
     9901 ggaatacaca agagcccgtc aggtaattga tacacgtgaa aaaagagtat ttggagaatg
     9961 cgcccgcatt gctcagacag gaacctccga aggaaccagc ctgcaaattg gcatccatgc
    10021 cagctttatc gtagatctgg ctactgctct ggcctataat accggagaac gtatgcttct
    10081 gattgtagaa aacaacggcg ctattgaaaa tttttcacct gacgccatgg tagaaatccc
    10141 ctgtatcgtg ggcaaagacg gatatgagcc tttatccgtc ggaaaaatcc ctactttcca
    10201 gaaggggctg atggaagagc aggttgctgt ggaaaagctg acggtagatg cctggatcga
    10261 gggaagctac cataagctct ggcaggctct taccctttct aaaaccgttc ccagcgctaa
    10321 tgtggcgaag aagctgctgg atgaatacat cgaggcaaac aaaggatact ggcctgactt
    10381 aaaataaacg gaggtactga aatggtaaac attgaaaaaa tggctgaaac tgccatgtac
    10441 atcgtgacct atgcaggcac ggctaaatcc tgctatatga atggactttc cgaagctaaa
    10501 aaggggaatt ttgaaaaagc ccgcgaactg ctgaaggaag gagataacaa tatggcacag
    10561 gcacaccagt cccatattgg ggttctggaa gaagaagtga catctcttac tccccagatt
    10621 tctcttctga tgtgccacgc cgaggatcag atcatgggag ccgaaacttt aaaaaccgtt
    10681 attattgaac tcattgaatt atatcagaaa aaggagaatt aattatggaa attcaaccac
    10741 aagattataa tatctgggcc ggatcacaaa cgagaaacgg ctttgccgca gatgaaatta
    10801 tatccagtct tcagaaagcc atacgcagag ccatggaaga acaggcttgc cagtatgctt
    10861 atgaactgta tgtatctggc cctattttcc ttgaaaagct ctggcgcaga cttcttacaa
    10921 tctctgttga agacattggc tttggaaatc tgaacgcggc tgtctatgta aacactatga
    10981 atgaaatgcg caaaaatttc ccttatgatg atggcgatca gcctatgtac ttcattcatg
    11041 cgatccgcat cctgtgttcc agcaataaag accgttccag cgacttctta aagaacatta
    11101 tcatcaaaga ggctgctatg ggcaaaaagc ctgaaattat ggatctggct cttgacaaac
    11161 atacggtaag aggcaaagct atgggtcgtg gttccaaaca tttctttgaa gaagcctgca
    11221 aggtattccc acaaatccct gtagacaacg actatagaga acgctacggc aaaattttgg
    11281 aggaatatga tccgtcaaaa aatgttcctg gcgctttcaa atataatcct gctcaggcat
    11341 aaaacagcct gagcagcaga aagcacctac ataaaacccg gcagtcccgg ctattgagac
    11401 ggactgtcgg gtttttgtat gg
//
LOCUS       DBMDUH010000081        11378 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_2870_length_11378_cov_5.281458, whole genome shotgun sequence.
ACCESSION   DBMDUH010000081 DBMDUH010000000
VERSION     DBMDUH010000081.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 11378)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11378)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11378
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2870_length_11378_cov_5.281458"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="ACZPCG_08025"
     CDS             <1..387
                     /locus_tag="ACZPCG_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HIR1285044.1"
                     /translation="QQALNELEEAGLIHKEASHSSTRYEITKEGEETLNFFGKNISPA
                     IVEDMDQYLKENKFRLREEVGTTADFYKGTNQDYIVHCEVRENKSVLIRLDLSVPDKD
                     QAEDMCSAWRSKSQEIYFHIMHTLLS"
     gene            426..977
                     /locus_tag="ACZPCG_08030"
     CDS             426..977
                     /locus_tag="ACZPCG_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HIR1285045.1"
                     /translation="MPLLKPRLYTSDDYWNLPENTRAELIDGQLYNMTPSSRIHQRIS
                     GQLYITIGTYIEEHRGNCQIYAAPFAVNLDCEDKNWVEPDLSVICDPDKLTDRGCSGA
                     PDLIIEIVSPSSRRMDYVQKPVLYLNSGVREYWIVDPVKKCTTVHLYDQDPAEFAPVI
                     FPFESDIRVGIYSDLCINISKLL"
     gene            complement(1025..1621)
                     /gene="yihA"
                     /locus_tag="ACZPCG_08035"
     CDS             complement(1025..1621)
                     /gene="yihA"
                     /locus_tag="ACZPCG_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009264492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HIR1285046.1"
                     /translation="MIIKSVDLETVCGVTSRLPENTLPEFAFAGKSNVGKSSLINALM
                     NRKSYARTSSQPGKTQTINFYNINQVLYYVDLPGYGYAKVSLEAKAKWGKMIERYLQK
                     SPMLKLVFLLVDIRHEPSANDKMMYDWVVSNGYHPVIIATKLDKLKRSQVPKAVKTIR
                     TGLGMEKEDILIPFSAETKQGREEIWELIESVALGTEE"
     gene            complement(1618..3933)
                     /gene="lon"
                     /locus_tag="ACZPCG_08040"
     CDS             complement(1618..3933)
                     /gene="lon"
                     /locus_tag="ACZPCG_08040"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007712006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HIR1285047.1"
                     /translation="MEERVITMPVVALRGLTILPGMVQHFDVNRPRSVAAVEKAMIGD
                     QKLFLVSQRHPEIPDPELKDLYQVGTIGVVKQLVKLPGKTVRVLVEGLERAELLDFDS
                     QEPCLIGEISSMEPEQEDADDLAKEAMVRIIREKLEEYGTVNLRAAKENLPSLLLIND
                     LSELLDQIAIQLPWDYTVRQNVLECTLLSARYETVLQTLLTEMEVYRIKKDFQEKVKA
                     DIDKNQKEYILREQMKIIRQELGEDTSLSDADDYQEKAEKLEAEKEVKEKLLKEIGRF
                     RTMPTGSQEANVLRTYIETLLELPWKKMSKDNDDILHAQEVLNEDHYGLEKVKERILE
                     YLAVRSLTKKGTSPIICLVGPPGTGKTSIARSVARALNKKYVRISLGGVRDEAEIRGH
                     RKTYVGAMPGRLAEGLRQAGVSNPLMLLDEIDKVSSDYKGDTSSALLEVLDGEQNVRF
                     RDHYIEVPIDLSQVLFIATANTTQTIPGPLLDRMEVIEVNSYTENEKFHIAKDYLVGK
                     QRERSGLTEEQIVFSDKSLEKIIHNYTREAGVRNLERRIGDVCRKAARGYLEDKKHSR
                     IRITESNLEKYLGKEKITFEDANEEDEVGIVRGLAWTSAGGDTLQIEVNVMPGKGNLL
                     MTGQLGDVMKESAQTALTYVRSICPEYGLADDYFEKHDLHIHIPEGAVPKDGPSAGIT
                     MATAMLSAVTGKKVLAKVAMTGEITLRGRVLPIGGLKEKILAAKMAHIKKVLVPEKNR
                     PDIGELSKEITKGLEIVFVKNMESVVKEAFA"
     gene            complement(4038..5348)
                     /gene="clpX"
                     /locus_tag="ACZPCG_08045"
     CDS             complement(4038..5348)
                     /gene="clpX"
                     /locus_tag="ACZPCG_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HIR1285048.1"
                     /translation="MPVRPDDTIRCSFCGRTQDQVRKMIAGTGDSNVYICDECIELCS
                     EILEEELEGDAEEASVLNDIHLLKPKEIKAFLDEYVIGQEAAKKVLSVAVYNHYKRVT
                     SCRHMDVDVQKSNILMIGPTGSGKTYLAQTLAKILNVPFAIADATALTEAGYVGEDVE
                     NILLKLIQAADYDVNRAEYGIIYIDEIDKITKKAENVSITRDVSGEGVQQALLKILEG
                     TVASVPPQGGRKHPHQELIQIDTTNILFICGGAFDGLEKIVEQRISAGSIGFNAEVVN
                     RNEMDIDELLRKVEPQDLTKFGLIPEFIGRVPVEVSLEQLTKEAMVRILSEPKNALTK
                     QYQKLFELDNVKLEFTKEALEEIARLAVERKIGARGLRSILESAIMDLMYEIPSDDSI
                     GICTITKAVIDKTGEPEFVYRDMQPAARKPLSERIRGGRNGEIA"
     gene            complement(5383..5964)
                     /gene="clpP"
                     /locus_tag="ACZPCG_08050"
     CDS             complement(5383..5964)
                     /gene="clpP"
                     /locus_tag="ACZPCG_08050"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HIR1285049.1"
                     /translation="MSLVPYVIEQTSRGERSYDIYSRLLKERIIFLGEEVNDTSASII
                     VAQLLFLEAEDPSKDIHLYINSPGGSVSAGFAIYDTMKYIKCDVSTICIGMAASMGAF
                     LLAGGTKGKRFALPNSEIMIHQPSGGARGQATEIKIVAENILKTKKKLNEILAANTGR
                     SIEEIERDTERDNYMSAEEAKAYGLIDEILTHH"
     gene            complement(6090..7376)
                     /gene="tig"
                     /locus_tag="ACZPCG_08055"
     CDS             complement(6090..7376)
                     /gene="tig"
                     /locus_tag="ACZPCG_08055"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HIR1285050.1"
                     /translation="MSVQVEKLEKNMAKLTVEVSAEDFKAAIKKAYNKTKNRFAIPGF
                     RKGKASQAVIEKMYGEAVFYEDAADEAINSTYAEAMKESGLDIVSRPEITVEQIGKDQ
                     AFIYSALVAVKPEVTLGEYKGVEVEKADAAVTAEDVEAELKRVQEQNARLLTVEDRPV
                     ADGDQTVIDFEGFVDGKGFEGGKAEDYPLTIGSHSFIDTFEEQLIGKNIGEECEVNVT
                     FPTEYHAAELAGKPAMFKVTVKEIKVKELPALDDEFASEVSEFDTLDEYKQDIEKKLQ
                     ERKEKAAASQNEDRVVAKVVENASMEIPEKMIDAQVDNMLRETAQRMQSQGLSMDLYM
                     KYTGMTADQMKDQMRPEAVKRIQTRLVLEAVVKAENIETSEEKLDEEIAKMAEAYKME
                     ADKLKSYMTDSDKDQMKQDLAVQAAVDLLVSEAKLV"
     gene            complement(7506..8063)
                     /locus_tag="ACZPCG_08060"
     CDS             complement(7506..8063)
                     /locus_tag="ACZPCG_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1 family glyoxalase III"
                     /protein_id="HIR1285051.1"
                     /translation="MSKVFAFVADGLEEVECLAVVDVLRRSGVDTELVSVMGRKDITG
                     AHGIHFQADVLFEEVNTDEADVLFLPGGMPGTSNLKAHEGLSEAICRANKQGRRLAAI
                     CAAPSILGEMDLLKGRTATCYPGFEDMLHGASHTLQGVVTDGNITTARGLGYALDLGL
                     ELVRLLLGSQHAAGLRAAIQYNQAK"
     gene            8196..8666
                     /locus_tag="ACZPCG_08065"
     CDS             8196..8666
                     /locus_tag="ACZPCG_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HIR1285052.1"
                     /translation="MEIERKYKIECLNDLPENYRAFPCHAIEQAYLCTDPVVRVRRED
                     DSYYLTYKGKGLLAREEYNLPLTKESYEHLLKKADGLVLTKVRWLLPLEGRTDLTIEL
                     DIFSGCYKGLILAEVEFPTIEDADSFTPPDWFGEDVTFSGLYQNSRLSNPHKQP"
     gene            complement(8644..9213)
                     /locus_tag="ACZPCG_08070"
     CDS             complement(8644..9213)
                     /locus_tag="ACZPCG_08070"
                     /inference="COORDINATES: protein motif:HMM:NF021028.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HIR1285053.1"
                     /translation="MEEEKKKLFGRGIYGSKDVPIRILDGLIVGMILLAVVLTVIFSI
                     NGGYRVTFDTRGGGEIPYQKLRYGKLVEKPEEPQKPGYEFVCWKREEAEGEWSFEHDQ
                     VKGDMTLTAQWKPASVLVKFDLDGGTVDGKTEAEPVFVTYGEVYGALPEPEKTGVSFK
                     GWIYSGQMIEADTRVAMTGEHVLTAVYED"
     gene            complement(9313..10392)
                     /locus_tag="ACZPCG_08075"
     CDS             complement(9313..10392)
                     /locus_tag="ACZPCG_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008371023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HIR1285054.1"
                     /translation="MSRLNIFAAIQGKIDYYKSLDAKDKTTYWKETLINNALYILLII
                     AIIYTYSQNHKFLSGNSIVNIVSLSAANIPIACGIAGCIVLTGTDLSAGRVVGLTACI
                     SASLLQSLSYATRMFPDLPVMPIWVVIPLVMVVGGIVGWVNGFFVAKFKLHPFIVTLA
                     TQLIVYGALLMYVMINGNNGQPLSGLDDSFKSFVTGSKLSLGGIPIPNYVWYAIVIVV
                     VMWFIWNKTAFGKNMFAVGSNPEAANVSGVNVNKTIIMVHTLAGMMYGLTGFIESARI
                     GSNTANTGLNYECDAIAACVIGGVSFVGGTGKISGVVLGVFLLRIIFVALNFLSINQN
                     LQYIIKGLIILVACSIDMRKYLVRK"
     gene            complement(10410..>11378)
                     /locus_tag="ACZPCG_08080"
     CDS             complement(10410..>11378)
                     /locus_tag="ACZPCG_08080"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009461927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR1285055.1"
                     /translation="EHLFDIINMLRDKGCGIIYISHKMAEILRISDEVTVMRDGTWVA
                     TEPAKELTTEKIIKLMVGRELNNQFPPKTNKAGEVALEVEHLSGQYSLLNDVSFNVRK
                     GEIIGLAGLDGSGRTEVLENLFGIATRKSGTIRLDGKQVLNRNARESIKNGFALLTEE
                     RRATGIFGILDIRENTVISNLKNYRSKAFPLMLDDKKMRQDTQWSIDAMHTKTPTQET
                     KIRSLSGGNQQKVILGRWLLTKPEVLLLDEPTRGIDVGAKYEIYQLIIDLANEGKVVI
                     MVSSEMPELLGVCDRILVMSGGRLAGEVDARNTTQEEIMTLAAKYV"
BASE COUNT         2741 a         3102 c         2398 g         3137 t
ORIGIN      
        1 cagcaggccc taaatgaact ggaggaagca ggactgatcc ataaggaagc cagccacagc
       61 agcacacgct atgagattac aaaggagggg gaagaaacct taaacttttt cggcaaaaat
      121 atatccccag ccattgtgga ggacatggat cagtatttaa aggaaaataa attccgactc
      181 cgtgaagagg taggaaccac ggctgatttc tataaaggca ccaatcagga ctatatcgtt
      241 cactgcgagg tacgggaaaa caaatccgtc ctgatccgtc tggatctgtc cgttccggac
      301 aaggatcaag ccgaggatat gtgcagcgcc tggcgctcta aaagccagga aatctatttc
      361 catattatgc atacgctgct gtcgtaacat aaacggaaat ttcgttttca gaaagaaacc
      421 attttatgcc tttgttaaaa cccagacttt atacatcgga tgattactgg aatctgccag
      481 aaaatacccg ggcagagtta attgacggac agctctataa catgactcct tccagccgaa
      541 tccatcagag aatatccgga cagctgtata tcaccattgg aacatacatt gaggaacatc
      601 ggggaaactg ccagatctat gccgccccct ttgctgtcaa tctggactgt gaggacaaaa
      661 actgggtaga gccggatctt tctgtcatct gcgatcctga caagctgaca gaccgtggat
      721 gctccggtgc tccggatctt atcatcgaga ttgtatcccc cagcagccgg aggatggatt
      781 atgttcaaaa acccgtcctg tacttaaatt ccggcgtccg tgaatattgg atcgttgacc
      841 cggtcaaaaa atgtacaact gttcatctct acgatcagga tcccgccgaa tttgcccctg
      901 ttatctttcc ctttgagagt gatatcaggg tggggattta ttcggatctt tgtattaata
      961 tcagtaagct gttgtaaaaa gggctccccg gctgaatgga tatagccggg gagtcccttt
     1021 ttctttattc ttctgttccc aacgccacac tctcaatcaa ttcccatatc tcctctctcc
     1081 cctgctttgt ctcagcagaa aaaggaatca ggatatcttc tttctccatt cccagccctg
     1141 tgcggatggt ctttacggcc tttggcacct ggctgcgttt gagcttgtcc agcttggtgg
     1201 cgatgatcac cggatggtag ccattgctca ccacccagtc gtacatcatt ttgtcattgg
     1261 cagagggctc gtggcggata tccaccagca gaaacaccag cttaagcatg ggggatttct
     1321 gcagataccg ctcgatcatc tttccccact tggccttggc ctccagagaa acctttgcgt
     1381 agccgtatcc aggcagatct acatagtaca gcacctgatt gatgttgtaa aaattgatgg
     1441 tctgcgtctt tcccggctgg gacgaggttc tggcatagga cttgcggttc atcagggcat
     1501 tgatcaggga agatttgccc acattggatt tgcctgcaaa ggcaaattcc ggcagcgtat
     1561 tttccggcag acggctggtg acgccgcata cggtttccag atctacggat ttaataatca
     1621 tgcaaatgcc tcctttacca ctgattccat gtttttaaca aatacgattt ccagtccttt
     1681 tgtgatctcc ttagaaagct ctccgatatc cggcctgttt ttctccggca caagaacctt
     1741 cttaatatgg gccatcttgg ctgccaggat cttttctttt aagcctccga taggcagtac
     1801 ccggcctctt aaggtaatct caccggtcat ggctaccttt gcaagaacct tctttcctgt
     1861 cacagcagac agcatggctg tcgccatggt aatgcctgct gatgggccgt cctttggcac
     1921 agcgccctcc gggatatgga tatgcaggtc atgtttttca aaataatcgt ctgccagccc
     1981 gtattccggg cagatagaac gcacataggt aagagccgtc tgagcggatt ctttcattac
     2041 atctcccagc tgtccggtca ttaaaagatt tccctttcca ggcatcacat tgacttcgat
     2101 ctgaagagta tcgcctccgg cgctggtcca ggccagtccc cgtacgattc ccacctcgtc
     2161 ctcctcattt gcatcctcaa aggtaatctt ttcctttccc agatattttt ccagattgct
     2221 ttccgtgatg cggatacggc tgtgcttctt atcctccaga taccctctgg cggccttgcg
     2281 gcacacgtct ccgatccggc gctctaagtt tctcactccg gcctctctgg tataattgtg
     2341 gataatcttt tccaggctct tatcggaaaa tacgatctgc tcctctgtca gtccgcttct
     2401 ctctctctgc tttcctacca gataatcctt tgcaatgtgg aacttctcat tttccgtata
     2461 gctgtttacc tcaatgacct ccatacggtc cagaagaggc cctggaatgg tctgggttgt
     2521 gttggccgtg gcaatgaaga gaacctggga taaatcaatg ggcacctcta tgtagtggtc
     2581 cctgaaccgc acattctgtt cgccgtctaa gacctctaaa agagccgatg aggtatcgcc
     2641 cttataatcg ctgcttacct tgtcaatctc atcaagaagc atcagcggat tgctcactcc
     2701 cgcctgacgc agtccctctg ccagacggcc gggcatagcg ccaacatatg ttttccgatg
     2761 gccccggatc tcagcctcat cccggacgcc acccagactg atgcgcacat actttttatt
     2821 cagcgctctg gctacggatc tggcaataga tgtttttccc gttcccggcg ggcctaccag
     2881 acagataatg ggactggtgc ccttctttgt aaggctgcgc acagccagat actccagaat
     2941 acgctccttt accttttcca ggccgtaatg gtcttcatta agcacttcct gggcatgaag
     3001 gatatcatca ttatcttttg acattttttt ccaaggaagc tctaaaaggg tttcaatata
     3061 ggtccgcagc acatttgcct cctggcttcc cgtgggcatg gtgcggaagc gtccgatttc
     3121 ctttaaaagc ttttccttta cttccttttc tgcctctaac ttctctgcct tttcctgata
     3181 atcatctgca tctgacaggg aagtatcctc tcccagctcc tgacggatga tcttcatctg
     3241 ctctctgagg atgtattcct tctgattctt atcgatatct gcctttacct tttcctgaaa
     3301 atcttttttg atccggtaaa cctccatctc ggtaagcagc gtctgaagta cggtctcata
     3361 gcgggcggaa agcagggtgc actcaagcac attctggcgc accgtataat cccagggaag
     3421 ctggatcgca atctgatcca acagctcaga caggtcgttg atcagaagaa gggacggcag
     3481 gttttccttg gcagccctta agtttacggt tccatactcc tccagttttt cccggatgat
     3541 ccgcaccatg gcttcctttg ccaagtcatc tgcatcctcc tgctccggct ccatggaact
     3601 gatctctccg atcagacagg gctcctgcga atcaaaatca agaagctccg cacgctcaag
     3661 cccttctacc agcacccgca ctgtctttcc cggcagcttc accagctgct ttacgactcc
     3721 aatggtacct acctggtata aatccttaag ctcaggatcc ggaatttccg gatgcctctg
     3781 gctgactaaa aacagcttct gatctccgat catggccttt tccaccgccg ctacggacct
     3841 gggcctgttt acatcaaaat gctgtaccat tcccggaaga atggtaagtc ctcttaaggc
     3901 cactaccggc atggttatca ctcgttcttc catctgtttg tcacctcgta tctttaaaaa
     3961 ggccgctgca aatgcaggaa tctggtttgc agcggtctga aatgttcttg ctaattttca
     4021 tcgatcaacg gtacgcctta agcgatctcc ccgtttcttc ctcctctgat ccgttcggat
     4081 aaaggctttc tggctgccgg ctgcatatct ctgtatacaa actccggctc ccctgtttta
     4141 tcgatcacag cctttgtaat ggtgcagata ccaatggaat catcggaagg aatctcatac
     4201 atcagatcca tgattgcgct ttccaaaatg gaacgcagtc ctctggctcc gatcttgcgc
     4261 tctaccgcca gtctggcaat ctcctccaat gcttctttgg taaactcaag ctttacattg
     4321 tcaagttcga acagcttctg gtactgcttt gtcagagcat tcttcggctc agacaggatg
     4381 cgcaccatag cttcctttgt cagctgctct aaagatacct ctaccggcac acggccgata
     4441 aactcaggga tcaggccgaa ttttgtcaga tcctgaggct ctaccttgcg cagaagctca
     4501 tcaatatcca tctcattgcg gtttaccacc tcagcattaa agccgataga gccagcgctg
     4561 atgcgctgtt ccacgatctt ttccagaccg tcaaaggcgc cgccgcagat aaagaggata
     4621 ttggtggtgt cgatctggat cagctcctga tgaggatgct ttctgcctcc ctgaggcgga
     4681 acgctggcaa cggttccctc caagatttta agaagcgcct gctgtacgcc ttcgccggac
     4741 acgtcgcggg taatggaaac attttctgcc tttttggtga tcttgtcgat ctcatcaatg
     4801 taaataatgc cgtactccgc acggtttaca tcgtaatcag ccgcctggat cagctttaag
     4861 agaatatttt ccacgtcctc gcctacatat cctgcctctg tgagggccgt agcatctgca
     4921 atggcaaagg ggacattcaa aatcttcgca agagtctgtg caagataggt tttaccggag
     4981 cccgtggggc cgatcatcag gatattgctc ttctgcacat ccacatccat atgcctgcag
     5041 gaggtgacgc gcttgtagtg attgtacacg gcaacggaaa gaaccttctt tgccgcctcc
     5101 tgtccgatca catactcatc cagaaatgcc ttgatctcct ttggctttaa cagatggata
     5161 tcatttaaga cagaagcctc ctctgcatct ccctctaact cctcctccag aatctcagag
     5221 cacagttcaa tacactcgtc acaaatatat acattactgt cgcccgttcc ggcaatcatc
     5281 ttgcgtacct gatcctgcgt ccttccgcag aaggaacagc ggatcgtgtc atctggtcta
     5341 actggcatac tatacctacc ctttcctatc tcctaactct tctcaatgat gtgtgaggat
     5401 ctcatcgatc aggccgtatg cctttgcttc ctcagcagac atatagttgt cgcgctccgt
     5461 atcccgctcg atctcctcaa tggatctgcc tgtattggct gccaggatct catttaactt
     5521 tttcttggtc tttaaaatgt tttcagctac aatcttgatc tctgtcgcct ggcctctggc
     5581 tccgccggag ggctgatgga tcatgatctc ggaattggga agagcaaagc gcttgccctt
     5641 tgttcctcct gcaagaagga atgcacccat gctggctgcc atgccgatgc agatcgtaga
     5701 cacatcgcat ttgatgtatt tcatggtgtc ataaatagca aagcctgcgg atacggaacc
     5761 gccggggctg ttgatataaa ggtggatatc cttggaagga tcctccgcct ccagaaacag
     5821 aagctgagcc acaataatgc ttgcggaagt atcatttacc tcttcgccca ggaatatgat
     5881 acgctccttt aacagcctgg agtagatatc gtaggaacgc tctcccctgc tggtctgttc
     5941 aatgacatag ggtactaaac tcatgtaacc atcctcctgt aattctgtat aataaaacac
     6001 ttattttcaa aatgaaacca gggagatcct ccctttcctg cgcctttcag acacacgaaa
     6061 gaagggatcc ccctgccatc ctgtgtggat taaaccagct tagcctcgga tactaacaga
     6121 tcaactgctg cctgaactgc aaggtcctgc ttcatctggt ccttatcgct gtccgtcatg
     6181 tagctcttaa gtttgtcagc ctccatcttg taggcctcag ccatctttgc gatctcctca
     6241 tctaacttct cctcagaagt ctcgatgttc tcagccttta caactgcctc aagaaccagt
     6301 ctggtctgga tgcgctttac agcctcagga cgcatctgat ccttcatctg atctgcagtc
     6361 atgcctgtgt acttcatgta cagatccatg gacagaccct ggctctgcat acgctgtgca
     6421 gtttcccgaa gcatgttgtc aacctgagcg tcgatcatct tctccggaat ctccatggag
     6481 gcattctcaa ctacctttgc aactacgcgg tcttcattct gggaagcggc tgccttctct
     6541 tttctctcct ggagcttctt ctcgatatcc tgcttgtact catccagagt atcaaactcg
     6601 gaaacctcag atgcgaactc atcatccagg gcaggaagct cttttacctt gatctccttt
     6661 acagttacct tgaacatagc aggctttcct gccagctctg ctgcatggta ctcagttggg
     6721 aaagtaacgt ttacttcgca ctcctcaccg atgttcttgc cgataagctg ctcctcaaag
     6781 gtatcgatga aggaatgaga accaatggtt aaaggataat cctcagcctt tccgccctca
     6841 aagcccttgc cgtctacaaa gccctcaaag tcgatgacag tctggtcacc gtcagctacc
     6901 ggacgatcct caacagtcag cagtcttgca ttctgctcct gtactctctt aagctctgcc
     6961 tcaacatcct cagcagttac agcagcgtct gccttctcaa cctcaacgcc cttgtactcg
     7021 cctaaggtaa cttccggctt aacagcaact aatgcgctgt agatgaatgc ctgatcctta
     7081 ccaatctgct ctactgtgat ctccggtctg gaaacgatat ccagtccgct ctccttcata
     7141 gcctcagcat aggtagagtt gatcgcttcg tcagcagcat cctcatagaa tacagcctca
     7201 ccgtacatct tctcgatcac agcctgagaa gccttgccct tacggaagcc ggggattgca
     7261 aaacggttct ttgtcttgtt gtaagccttc ttgatcgcag ccttaaaatc ttctgcgctg
     7321 acttcaacgg tcagcttagc catatttttc tctaactttt ctacctgtac actcattatt
     7381 acgggttcct ccttaaaata tatgtgattc gcccataacc atgagggcgc tacacttcaa
     7441 tgtagcatta tatcatggag acgggaaaaa ggctagtatt ttttgaaaaa gttaagcaga
     7501 tccgtctatt ttgcctgatt gtactggatc gcagccctaa ggccggcagc atgctgggat
     7561 cctaaaagaa ggcgtaccag ttccaggccc agatccagcg catagccaag tcctctggct
     7621 gtagtgatat ttccgtctgt cactacgccc tgcagcgtat gggaagcccc gtgaagcatg
     7681 tcttcaaagc caggataaca ggtggcagtc cgtcctttta acagatccat ctctcccagg
     7741 atactgggag ccgcgcagat ggccgccagc ctcctgccct gcttattagc acggcagatg
     7801 gcttcagaca atccctcatg tgcttttaag ttgctggttc cgggcatccc tcccggaaga
     7861 aacagtacgt ccgcctcatc cgtatttacc tcttcaaaaa gaacgtctgc ctgaaagtgg
     7921 attccgtgag cgccggtaat gtctttccgg cccatcaccg ataccagttc tgtatccacg
     7981 ccggaacgcc tcaaaacatc caccactgca agacactcta cttcttccag tccgtctgcc
     8041 acaaatgcaa aaacctttga cataatatat tctcccttta tcttatattg acattatttt
     8101 atcatttctc cctttccctg aaaagagata aatgatatga tagtctctgc gaaaagggat
     8161 aaaatataga taaatctcac aaaggagtgt tttaaatgga aatcgaacgt aaatacaaga
     8221 tcgaatgtct gaatgatctg ccggaaaatt accgggcctt tccctgccat gccattgagc
     8281 aggcctatct gtgcactgat cccgtggtgc gtgtgcgccg ggaggacgat tcctattatc
     8341 tcacctacaa aggaaaagga ctccttgcaa gggaagaata taatcttcct ctcacaaagg
     8401 agtcctatga acatctttta aaaaaagccg acggcctggt cctcacaaaa gtgcgctggc
     8461 tccttcccct ggaaggaaga accgatctga ccattgaact ggacatcttt tccggctgct
     8521 acaagggact gatcttagcg gaggtggaat ttcctaccat agaggatgca gattctttta
     8581 cgccgccgga ctggtttggg gaggatgtta ccttttcggg actgtatcag aacagccgcc
     8641 tttctaatcc tcataaacag ccgtaaggac atgttcgcct gtcatggcta ctcttgtgtc
     8701 agcttcgatc atctgaccgc tgtaaatcca tcccttaaag gatacgcccg ttttttcagg
     8761 ttccggaagt gctccgtaca cttccccata ggtcacaaaa accggttctg cctctgtctt
     8821 tccatctacc gttcccccat ccagatcaaa cttaaccaga acagaggcag gcttccactg
     8881 ggcagtcagc gtcatatctc cttttacctg atcgtgctca aacgaccact ccccttctgc
     8941 ctcttctctc ttccagcaca caaattcata tcctggcttc tgaggttcct caggcttttc
     9001 cactaatttt ccatagcgca gcttctgata ggggatttct ccgccgcccc gggtgtcaaa
     9061 agtcacacgg tatcctccgt tgatactgaa aataacggtc agcacaactg ctaaaaggat
     9121 cattcccacg atcaggccat ccaggatccg tatgggcaca tccttgcttc cataaatccc
     9181 tctgccaaaa agctttttct tctcttcttc catgcggaaa cctccttgtg tcacattcat
     9241 acgataatcg ttcagacaaa aattgctgcg gatgcgtaca atccgcttcc gcagcaataa
     9301 aaattcagct tattattttc ttaccagata ctttctcata tcgatggagc atgccaccag
     9361 aatgataagt cccttgataa tgtactgcag gttctggtta atggacagga agttcaacgc
     9421 tacgaaaatg atccgcagaa ggaacacgcc cagtaccacg ccgctgatct taccagtacc
     9481 gcctacaaag gatacaccgc cgattacaca ggctgcaatc gcatcacact catagttcag
     9541 acctgtattg gcagtattgg aaccgatacg tgcagactcg ataaaaccgg tcagaccata
     9601 catcatacct gccagagtat gtaccataat gatcgtcttg tttacattaa caccggatac
     9661 attggccgct tccggattgg aacctacggc aaacatattt ttgccaaacg cagttttgtt
     9721 ccagataaac cacattacga ctacgataac aattgcatac catacatagt ttggaatcgg
     9781 aatgccgccc agagacagct tgctgcctgt tacaaagctc ttaaaggaat catccaggcc
     9841 ggaaagaggc tggccgttgt ttccattgat cataacatac atcagcagag cgccgtatac
     9901 gatcagctgg gtagccaaag taacgataaa gggatgaagc ttaaacttgg ctacgaaaaa
     9961 accgttgacc catcctacaa tacctcccac aaccataacc agaggaatta caacccagat
    10021 aggcataacc ggaaggtcag ggaacattct tgttgcataa cttaaggact gcagcaggga
    10081 tgcggaaata caggcagtca aacctacaac gcgtcctgca gaaaggtctg taccggtcag
    10141 tacaatacat cctgcaatac cacaggcaat ggggatgttt gctgctgaaa ggctgacaat
    10201 gtttacaatg gagtttcctg aaaggaactt gtggttctga gaataggtgt aaataatagc
    10261 aatgatcagc aggatgtaaa gcgcattgtt gatcagggtt tccttccagt aggtggtttt
    10321 atcttttgca tccagacttt tgtagtaatc tattttcccc tggatagctg cgaaaatatt
    10381 cagtcgactc atttgtccat atcccccttt tatacatatt tagctgcaag tgtcataatt
    10441 tcttcctgag tggtatttct ggcgtcaact tcccctgcca gccgtcctcc tgacatcacc
    10501 agaatacggt cgcatactcc taaaagctcc ggcatttctg aggataccat gatgactacc
    10561 ttgccttcat ttgccaggtc aatgatcagc tgataaatct catatttcgc acctacgtcg
    10621 attcctcgtg taggctcgtc cagaagcagc acctctggct ttgtaagaag ccatcttccc
    10681 agaataacct tctgctggtt tcctccggaa agactgcgga tctttgtttc ctgggtaggc
    10741 gtctttgtat gcatggcgtc aatggaccac tgggtatcct gtctcatctt tttgtcgtcc
    10801 agcatcaaag gaaatgcctt gctccgataa tttttaaggt tcgaaataac cgtattttca
    10861 cgaatatcca gaataccgaa aataccggtt gcacggcgct cctcggtcaa aagggcaaaa
    10921 ccatttttga tggactctct tgcattccgg tttaagacct gcttcccgtc cagacggatc
    10981 gttcccgact ttctggtggc aataccaaac agattctcaa gaacctctgt ccgtccgctt
    11041 ccatccaggc ctgccagtcc aatgatctca ccctttctta cattaaagga aacatcattt
    11101 aaaagagaat actggccgct taaatgctct acctcaaggg ccacctctcc tgccttgttt
    11161 gtctttggcg ggaactgatt gttcagttca cggcctacca tcagtttgat gattttttct
    11221 gtggtcagct cctttgccgg ctccgtggct acccaggtac cgtcgcgcat aactgttact
    11281 tcatcagaaa tacgcaggat ttctgccatc ttatgggaaa tgtaaataat gccgcagccc
    11341 ttgtcacgaa gcatattgat gatatcaaag aggtgctc
//
LOCUS       DBMDUH010000082        11365 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_2876_length_11365_cov_5.898615, whole genome shotgun sequence.
ACCESSION   DBMDUH010000082 DBMDUH010000000
VERSION     DBMDUH010000082.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 11365)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11365)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11365
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2876_length_11365_cov_5.898615"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(12..620)
                     /locus_tag="ACZPCG_08085"
     CDS             complement(12..620)
                     /locus_tag="ACZPCG_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HIR1285056.1"
                     /translation="MKGIMKDAFLLFVITLVAGACLGAVHEVTLEPIAKAQLAANTAT
                     YKEVFPEAASFEQTDELKAAVEAGAAQIAEQGFGNVSIDAAQEALAEDGTLLGYLITA
                     SSNDGYNGLVQISAGITAEGTLTGIGFLSISETPGLGMKASEPEFKGQFTGKAAQTLE
                     VTKTAPSADNQIQAISGATYTSNAVTGAANAAIYFVENCIGE"
     gene            complement(617..1558)
                     /locus_tag="ACZPCG_08090"
     CDS             complement(617..1558)
                     /locus_tag="ACZPCG_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009218334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HIR1285057.1"
                     /translation="MNKLLNVSSSPHVRCSVDTQSLMLDVAIAMLPAAAFGVYNFGFH
                     ALFVILATVAACIASEYVYEKLMHRPITIMDGSALVTGMILALNMPPEIPVWIPVIGG
                     IFAIVVVKQLYGGLGQNFMNPALAARCFLLISFAGHMNNFSSAKLGFDSVSGATPLAS
                     MRAGGSVDLAAMVVGRIPGTIGEVSVIALLIGGIYLIVKKVISWKIPVTYIATLAVFV
                     FIFGGLDANYVLAQVCGGGLIFGAFFMATDYVTSPITPMGQIVFGIMLGLLTGIFRLW
                     GASPEGVSYAIILCNLFVPMIERVTLPKAFGKEAKKA"
     gene            complement(1574..2899)
                     /gene="rsxC"
                     /locus_tag="ACZPCG_08095"
     CDS             complement(1574..2899)
                     /gene="rsxC"
                     /locus_tag="ACZPCG_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HIR1285058.1"
                     /translation="MGLATFKGGIHPYEGKELSESKPIQVLLPKGELVYPMSQHIGAP
                     AKPLVAKGDRVLAGQKIGEAGGFISANVICSVSGTVKAIEPRRTANGAMVPSVIVEND
                     GAYETVEGLGQDRNPGTLSKEEIRSIVKEAGIVGLGGAGFPTHVKLTPKDENAIDYVI
                     VNGAECEPYLTSDYRLMVEEPEKLVGGLKVILQLFDNAKGVIAVENNKPEAIKKLEEL
                     VKDEPRITVCPLLTKYPQGGERSIIYAVTGRKVNSSMLPADAGCIVDNVDTVISIYMA
                     VCKSTPLMRKIITITGDAVNTPCNFNVKLGTNYQELIEAAGGFKEEPEKIISGGPMMG
                     QALFTVDIPVSKTSSALTCFTKDQVSALEPTACIRCGRCVSVCPSRIVPVMMMQAALR
                     NDCERFEKVNGMECMECGSCTYICPARRPLTQAFKEMRKTVAANRRKKG"
     gene            complement(3167..3793)
                     /locus_tag="ACZPCG_08100"
     CDS             complement(3167..3793)
                     /locus_tag="ACZPCG_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HIR1285059.1"
                     /translation="MKVLMLNGSPHKEGCTWRALCEIAKELEKEGIESEILHIGGQQV
                     WGCMGCGACGTLGKCVHDEDLVNTAVAKMKECDGLIVGSPVHYASASGAITSFLDRFF
                     YCGGSAAAHKPGAAIASARRAGTTATLDQLNKYFTFNQMPVVSSQYWNMVHGRSAEET
                     VQDEEGMQIMRTLGRNMAWLLKCIEAGRKAGITVPEAEPEKKRTSFIR"
     gene            complement(3830..7009)
                     /locus_tag="ACZPCG_08105"
     CDS             complement(3830..7009)
                     /locus_tag="ACZPCG_08105"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR1285060.1"
                     /translation="MKIEKMETSSFWKGEAGVKGLVEDQAHTYKVQLYLKNDQVRDYS
                     CTCAEGNSYRGICAHGEALFAYYKKYKKEALKPPIRTSFQAHAMIREYTNRAVADILE
                     EEEGSQVRIEPVLFLEGRDIQAEFKIGRERMYALRDLPAFCTALEGGAYVSYGKELAF
                     HHQRTVFEPVSRLLVDLILELMEHQKALRRVTLGRLDRDRFFEILLKNTSGEKGLEVR
                     LPQGSGGIFSLECSEPELAVLVSRYGRDGIRVRLEGVLEQDGEQETPQAVQAVFGGSR
                     HLYLAAGKRLYCCGEAFSRSAAIFLEQIRTDRESQILIADKDIPLFQQRVLKRIQGYS
                     RIRLEEVELDQYEPEPLKAEFYFEAGEDGTLYMEPYLSYGEYRFHPIEDEALPRTVCR
                     DVPGEFKISQVIGTYFKCKSSTDGRLVIRKDEDALYRLLGTGMDEFKALGEVYLAENM
                     KNWRVIRTPGIKAEVRLAAGWLELTVDPEEFSVKELTKILEAYSQKKKYYRLKTGQFL
                     ELGEGGIYTVMRLAKELGITKKELEAGQIRLPSYRALYVDHLLKEGSGVALYRDRLFK
                     AVIRGIRSVEDSSDPLPEALEGVLREYQKTGYRWMKTLDQYGFGGILADDMGLGKTLQ
                     TIALLEDAYSRGETLPSLIVCPASLVYNWECEIRRFAPKRRVWSLAGSSTARGELLQS
                     VRENPSFCQILITSYDLLKRDISLYQDIRFRFQIIDEAQYIKNSATQSARAVKAVSAQ
                     SRFALTGTPVENRLGELWSIFDYLMPGFLFGPSVFHKEYEVAVLRDQDQEALLKLKRL
                     IGPFVLRRVKKDVLKELPDKIEQVVYSHLEGEQRMLYVAKALELKRTFSQDMPGNGAK
                     LQILSGLMRLRQICCDPGLCLEGWRSGSAKLETCMDLIRRGTEGEHKILLFSQFTSML
                     ERISRRLEKEGIESICLTGAVPKEERLKLVERFSKENVPVFLISLKAGGTGLNLTAAD
                     IVIHYDPWWNVAAQNQATDRAHRIGQEKQVTVYRLITKDTIEENILNLQEAKRALADQ
                     IVTGEGISLSDLTAKELLDCIGEISEDSV"
     gene            complement(7046..8833)
                     /gene="argS"
                     /locus_tag="ACZPCG_08110"
     CDS             complement(7046..8833)
                     /gene="argS"
                     /locus_tag="ACZPCG_08110"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002573535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HIR1285061.1"
                     /translation="MKKILDLMEQKLKAAFTACGYEDKFAKVVLSNRPDLCEYQCNGA
                     MAAAKAYKTKPIDIANAVVEKLTAEREQPMFGEAVAVMPGFINLKLSEAFLADYMKGM
                     AEADKLGLEEPEKKETIIVDYGGANVAKPLHVGHLRSAIIGESIKRMGRFMGYEMIGD
                     VHLGDWGLQMGLIIEELRDRKPELVYFDESYTGEYPTEAPFTISELEEIYPAASAKSK
                     VDEAFKERAHQATLKLQKGYKPFRAIWDHILAVSVADLKKNYSNLNVDFDLWKGESDA
                     EPYIDHMVQMLIDKGLAYESQGALVVDVAQEGDTKEIPPCLIRKSDGASLYATSDLAT
                     IVEREQDFKPDRYIYVVDKRQAMHFEQVFRVSRKAEIVKPDTKMIFLGFGTMNGKDGK
                     PFKTREGGVMRLEKLISDIDEAVFNRIMENRTVSEEEARETAKVVGLAALKYGDLSNQ
                     ASKDYIFDMDRFISFEGNTGPYILYTIVRIKSILGKYKENGGSVSELAAEKKILPAAG
                     ASEKALMLQLAKYSEVLENSFAETAPHKICQYVYETANAFNSFYHDTKILSGEDEARK
                     DSYIGLITLTKRVLETCIGLLGIEAPERM"
     gene            complement(8927..9484)
                     /gene="efp"
                     /locus_tag="ACZPCG_08115"
     CDS             complement(8927..9484)
                     /gene="efp"
                     /locus_tag="ACZPCG_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008724890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HIR1285062.1"
                     /translation="MISAGDFRNGITLEIEGQVVQILEFQHVKPGKGAAFVRTKLKNV
                     INGGVVERTFRPTEKFPAARIDRVDMQYLYADGDLYNFMNTENYEQLALNQDLIGDAL
                     KFVKENEICKVCSYNGNVFSVEPPLFVELEITDTEPGFKGDTATGASKPATVETGAVV
                     SVPLFVEIGDRIKIDTRTGEYLSRV"
     gene            complement(9597..10118)
                     /locus_tag="ACZPCG_08120"
     CDS             complement(9597..10118)
                     /locus_tag="ACZPCG_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007713285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HIR1285063.1"
                     /translation="MFEPFYPQEIWDSTYDIPFETWKDRGIKGVIFDIDNTLVPHGAP
                     ADERAIALFEHLKKLGFKTCLLSNNKEARVKPFASQVKSPYIHKGGKPSKRGYKNAMA
                     CMGTGPKETLFVGDQLFTDVYGANRAGLKSILVKPIDPKEEIQIVLKRYLERPVLYFY
                     RKSRKKNLKNMDT"
     gene            complement(10137..10589)
                     /gene="nrdR"
                     /locus_tag="ACZPCG_08125"
     CDS             complement(10137..10589)
                     /gene="nrdR"
                     /locus_tag="ACZPCG_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008724885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HIR1285064.1"
                     /translation="MKCPYCNEADTKVIDSRPADDNSSIRRRRQCERCGKRFTTYEKL
                     ETMPLMVIKKDNTREAYDRSKIEAGIIHSCHKRPVSPQQIGVMIDEIENQIFNREERE
                     ISTAVIGELVMEKLKQVDEVAYVRFASVYREFKDVSTFMEELGKLLKK"
     gene            10776..>11365
                     /gene="aroD"
                     /locus_tag="ACZPCG_08130"
     CDS             10776..>11365
                     /gene="aroD"
                     /locus_tag="ACZPCG_08130"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HIR1285065.1"
                     /translation="MNCVYVRNLRLGEGTPKVCIPLVGASDEALFEEIKALSSYPADL
                     VEWRTDYMNDIFNEGRLTGLLPRLRKHLGNTPLIFTFRTTKEGGQRQASPQQYKTLAM
                     EAIASGCIDLIDIELFSDPDSITELTALAKEKGVKTIFSSHDFYSTPSCGELLSRLRH
                     MEKLGADIAKIAVMPRCPEDVLTLLTATVTARRQLT"
BASE COUNT         2723 a         3150 c         2366 g         3126 t
ORIGIN      
        1 tcttacgcct cctactctcc gatgcagttt tctacaaaat agatggccgc atttgccgct
       61 ccggttactg cattggatgt ataagttgcg ccgctgatgg cctgaatctg gttgtcagcc
      121 gaaggcgctg tttttgttac ctccagagtc tgggctgcct ttcctgtaaa ctgtccctta
      181 aactccggct ctgaagcctt catgccaaga ccaggagtct cgctgatgct taaaaagccg
      241 atacctgtca gcgttccctc cgcagtaatg cctgccgaga tctgcaccag gccattgtaa
      301 ccgtcattgg agcttgcggt aatcaggtag ccaagcagag ttccgtcctc agccagagcc
      361 tcctgggctg cgtcaatgga tacgtttcca aagccctgct ctgcaatctg ggctgctccg
      421 gcctctactg ctgcctttaa ctcatcggtc tgttcaaagg aagccgcctc aggaaatacc
      481 tccttgtagg tagctgtatt ggctgccagc tgggcctttg cgataggctc taaggtcacc
      541 tcgtggactg cacccagaca ggcgcctgct accagtgtga tgacaaagag aagaaatgca
      601 tctttcataa ttcctttcat gcctttttag cctcctttcc gaatgctttt ggaagggtca
      661 cgcgctcgat catgggcacg aaaaggttgc ataggatgat agcgtaggaa acgccctcag
      721 gagaagcgcc ccacagacgg aagataccgg ttaaaagtcc cagcatgatt ccgaatacga
      781 tctgtcccat aggtgtaatg gggctggtaa cataatcggt cgccataaag aacgctccga
      841 agatcagacc gccgccgcat acctgggcaa gcacataatt ggcgtcaagg ccgccaaaga
      901 taaatacaaa tacagccagg gtagctatgt atgtcacagg aatcttccag ctgatgactt
      961 tctttacgat caggtagatg ccgccgatca aaagtgcgat cacagaaacc tcaccgatgg
     1021 ttccgggaat ccgtcctaca accatagcgg ccagatccac gcttccccct gcccgcatgg
     1081 aggctaaagg agtggctcct gagacgctgt caaagcccag ctttgcggat gagaaattat
     1141 tcatatggcc cgcaaaggag atcaggagaa agcatcttgc tgccagagcc gggttcatga
     1201 agttctgtcc caggccgccg tacagctgct ttactacgac gatcgcaaaa ataccgccga
     1261 tcacaggaat ccaaaccgga atctccggag gcatattcag cgccaggatc atacccgtta
     1321 ccagggcgct gccgtccata atggtaatgg gacggtgcat cagcttctca tatacatatt
     1381 cgcttgcaat acaggcagct acagttgcca gtatcacaaa cagcgcatga aagccaaagt
     1441 tgtatacgcc aaaggctgcc gcgggaagca tggcgatggc cacgtccagc atcaggctct
     1501 gtgtatccac gctgcatctc acatgaggag atgaagatac atttaataat ttattcatga
     1561 cttttgtacc ctcctaccct ttcttcctgc ggtttgccgc aacggtcttt ctcatttcct
     1621 taaaggcctg agtcagagga cgtctggccg gacagatata ggtgcagctt ccgcactcca
     1681 tacactccat gccgttgacc ttctcaaaac gctcacagtc atttctcaaa gctgcctgca
     1741 tcatcatcac aggaacaatg cggcttggac atacgcttac gcagcggccg cagcggatgc
     1801 aggccgtagg ctccagggca gatacctgat cctttgtaaa acaggtcagc gcagaggaag
     1861 ttttgcttac agggatatcc acggtaaaga gtgcctgtcc catcatggga cctccggaaa
     1921 taatcttctc cggctcctcc ttaaagccgc ctgcagcctc gatgagctcc tgataattgg
     1981 ttcccagctt tacattgaag ttgcagggcg tattgaccgc atcgccggta atggtgatga
     2041 tcttgcgcat cagaggcgtg ctcttgcata ccgccatata aatagaaata actgtatcta
     2101 cattatctac aatgcagcct gcatcggcag gaagcataga ggaatttacc tttcttccgg
     2161 ttactgcata gataatggaa cgctcaccgc cctgaggata cttggtaagc agaggacata
     2221 cggtaatcct tggctcatcc tttacaagct cttccagctt tttaatggcc tccggcttat
     2281 tgttctccac cgcgatcacg cctttggcat tgtcaaagag ctgaaggatg acctttaagc
     2341 ctcctactaa cttctccggc tcctctacca tcagacggta gtcgctggtc agataaggct
     2401 cgcactccgc tccgtttaca ataacgtaat caatggcgtt ctcgtccttt ggggtaagct
     2461 ttacatgagt cgggaagcct gcgccgccca gtcccacgat gcctgcttcc ttgacaatgg
     2521 aacggatctc ttcttttgac agtgtccccg gatttctgtc ctgtcccagc ccctctaccg
     2581 tctcataggc tccgtcattt tcaacgatca cagacggaac catagcgccg ttggccgtcc
     2641 gtctcggctc gatggccttt actgtaccgg acacggagca gattacattt gcagaaataa
     2701 atccgcctgc ctctccgatc ttctgtcccg ctaaaacccg gtcgcctttt gcaaccagcg
     2761 gttttgcagg agcacctatg tgctgagaca taggataaac cagttcgccc tttggaagca
     2821 ggacctgaat cggtttgctt tcagacagtt cttttccttc atacggatgg ataccgcctt
     2881 taaaagtcgc cagacccata gatttttccc ttctttcttg tacattttct ctttttttgt
     2941 gaatctttca cactttttat agatctttca cagccttatc agtatagcat aaataaagcg
     3001 tgtatacaat atgtaaacca ctcgttaaat ttttaattat tttgtttatt ttttatcaat
     3061 taagcattgt tttctaattt gttcacttta atccaaaaaa tgcagcagaa ctatttcgct
     3121 ctgctgcatt ccaatctacg gctgtgacat ctttcgtctc agacatttaa cggataaagc
     3181 tggtgcgctt tttctccggt tccgcctcag gaaccgtaat tccagccttt cttccggcct
     3241 caatgcactt taaaagccat gccatattcc ggccaagggt acgcatgatc tgcattcctt
     3301 cctcgtcctg aacggtttcc tctgcacttc ttccatggac catgttccaa tactgggatg
     3361 aaaccaccgg catctgatta aaggtaaaat atttatttaa ctggtccaga gttgctgtgg
     3421 ttcctgctct tctggcggag gcgatggccg ctcccggctt atgagctgca gcgcttccgc
     3481 cgcagtagaa gaaacggtct aaaaaggagg ttatggctcc gctggcagat gcataatgaa
     3541 ccggagagcc aacgatcaga ccgtcgcatt ccttcatctt agcgactgcc gtatttacca
     3601 gatcctcatc atggacacat tttcccagtg tgccgcaggc tccgcagccc atgcagcccc
     3661 atacctgctg tccgccaatg tgaagaattt cagactcaat tccttccttt tccagttcct
     3721 ttgcaatctc acacagcgct ctccatgtgc atccctcttt gtgcgggctt ccgtttaaca
     3781 tcaatacttt catattctat atcctcctgc ttgatttttt attttcttct taaacagaat
     3841 cttctgatat ctctccgata cagtccaaaa gctcttttgc ggtaagatca gacagggaaa
     3901 tcccttcccc tgtgacaatc tgatctgcca gcgctctctt tgcctcctga aggtttaaaa
     3961 tattttcctc aatggtatct ttggtaatca gacggtaaac tgtgacctgt ttttcctggc
     4021 caatgcggtg agccctgtct gtggcctggt tctgggcggc cacattccac cagggatcat
     4081 aatggatcac aatatccgct gctgtaaggt ttaaccccgt tcctcctgcc ttgaggctga
     4141 tgagaaaaac aggaacattc tcctttgaaa accgctccac cagctttaat cgctcttcct
     4201 tcggcacggc tcctgttaaa cagatactct caatcccttc tttttccagc cggcggctga
     4261 tccgctccag catagaggta aactgagaaa aaagaaggat tttatgttct ccctctgttc
     4321 cccgccggat cagatccata caggtttcca gcttagccga accggatctc catccctcca
     4381 ggcacaatcc gggatcgcag cagatctgcc gcaggcgcat aagaccggaa aggatctgaa
     4441 gctttgcccc atttcccggc atatcctgag aaaatgtacg tttcagttca agggctttag
     4501 ccacataaag catcctctgc tctccctcca gatgggaata taccacctgc tcgatcttat
     4561 caggaagctc ttttaataca tcctttttca cacggcgcag cacaaaggga ccaatcagcc
     4621 gcttcagctt caaaagcgcc tcctgatcct gatctcttaa aaccgccacc tcatactctt
     4681 tatgaaaaac cgacggacca aataaaaagc ctggcatcag gtaatcaaaa atgctccaca
     4741 gctctcccag cctgttttcc accggcgtcc ccgtaagcgc aaaccgtgac tgtgcgctga
     4801 ctgcctttac cgcacgagcg ctctgtgtgg ctgaattttt aatatactgg gcctcgtcaa
     4861 taatctgaaa tcggaaacgg atgtcctgat aaagagaaat atcccgtttt aaaagatcgt
     4921 aggatgtgat caggatctgg cagaacgaag gattttccct tacagactgc agcagttccc
     4981 ctctggccgt actggagccg gccaaagacc agacgcgccg tttcggagca aacctcctga
     5041 tctcacattc ccaattatat accagagaag ccggacagac aataaggctg ggaagcgtct
     5101 ccccccggct gtaggcatcc tccaaaagag caatggtctg aagcgttttt ccaagcccca
     5161 tatcatccgc taaaatcccg ccaaacccgt actgatccag agttttcatc cagcgatatc
     5221 ctgttttctg gtattctctc agcacacctt ccaaagcctc agggagcgga tctgagctgt
     5281 cttctacaga acggatcccc cggatcacag ccttaaacaa ccggtctctg taaagcgcca
     5341 ctcctgaccc ctcttttaaa agatgatcca catacaaagc ccgataggac gggagccgga
     5401 tctgtcctgc ttccagttct tttttcgtta tgccaagttc ctttgccagg cgcatcactg
     5461 tatagatgcc cccttctccc agctctaaaa actgtcctgt ttttaaacgg taatacttct
     5521 ttttctggct gtaggcctcc aggatttttg tcagttcctt cacagaaaac tcttccggat
     5581 ccaccgtaag ctccagccac ccggctgcca gacgtacctc tgcttttatc cctggtgttc
     5641 ggatcactct ccagtttttc atattttctg ccagataaac ctctcccaga gccttaaatt
     5701 catccatccc ggtacccagc agacgataca gggcatcctc atccttgcgg atcaccaaac
     5761 gtccgtctgt gctgcttttg catttaaaat acgttccaat gacctggctg atcttaaatt
     5821 cccccggtac atcccgacag accgttctgg gaagcgcttc atcttctatg ggatgaaacc
     5881 tgtattctcc ataagacaga tatggctcca tatagagcgt tccatcttct cctgcttcaa
     5941 agtaaaactc tgcctttaac ggttccggct catactggtc taattccact tcctccagac
     6001 ggatccggct gtatccttgg atccgtttta atactcgctg ctgaaaaaga ggaatatcct
     6061 tatctgctat caggatctgg ctttctctgt ctgtgcggat ctgttccagg aaaatcgctg
     6121 ccgaacgtga aaatgcttca ccgcagcaat acagacgctt tccggctgca agatataaat
     6181 gacggcttcc tccaaaaact gcctgcactg cctgaggggt ttcctgttca ccatcctgtt
     6241 ccagaacccc ctccaaacgt acccgaatac catctctccc gtaccggctt accagaaccg
     6301 ccagttccgg ctctgagcat tccagagaaa atatccctcc gctgccctga ggcaggcgaa
     6361 cctccagccc tttttctcct gatgtatttt ttaataaaat ctcaaaaaac cgatcccggt
     6421 ccagtctgcc taaagtcacg cgtctgagag ccttctgatg ctccataagc tccaggatca
     6481 gatccacgag cagcctcgat accggttcaa agaccgttct ttgatgatgg aaggccagct
     6541 cctttccata ggatacgtaa gctcccccct ctagggctgt acagaaggcc ggaagatctc
     6601 tcaacgcata catacgctct cttccgatct taaattccgc ctgtatatcc cggccttcca
     6661 gaaacaacac tggttcgatg cgtacctggg agccctcctc ttcttccaga atatccgcta
     6721 ctgcacggtt tgtatactcc cggatcatgg catgagcctg aaaagaagta cggatcggag
     6781 gcttcagggc ttccttttta tatttcttat aatatgcaaa aagggcctcc ccatgggcgc
     6841 agatgcccct gtaagaattc ccttctgcac aggtacagga ataatccctt acctggtcat
     6901 ttttaagata cagctgaacc ttataagtat gggcctggtc ttctaccagg ccctttacac
     6961 cagcctctcc tttccaaaag ctgctggttt ccattttttc aatcttcatt gacatgaaat
     7021 ccatcctgcc tttacaggct ctggtttaca tccgctccgg cgcttcgatc cccagaaggc
     7081 cgatacaagt ctcaagcacc cgcttagtaa gggtgatcag gccgatgtag ctgtcctttc
     7141 tggcctcatc ttccccagag aggatcttgg tatcgtggta aaaactgtta aatgcattag
     7201 cagtctcata gacatactgg cagatcttgt gcggagctgt ttctgcaaag ctgttttcca
     7261 gtacctcgct gtattttgcc agctgaagca taagggcctt ttcagaagct cctgctgccg
     7321 gaaggatctt tttctcagcg gccagctcgc tgacacttcc tccattttcc ttgtattttc
     7381 caagaatgga cttaatacgc acaatggtat aaaggatgta gggacctgta tttccttcaa
     7441 aggaaataaa ccgatccata tcaaatatat aatccttaga agcctgattg gacagatcgc
     7501 catactttaa cgcagcaaga cccactacct ttgcagtctc ccttgcctcc tcttctgata
     7561 cagtacggtt ttccatgatg cggttaaata cagcctcatc aatatctgag atcagctttt
     7621 ccagacgcat cacgccgcct tctcttgttt taaaaggctt tccgtccttg ccgttcatgg
     7681 tgccgaagcc caaaaagatc atctttgtat ccggctttac gatttctgcc tttctggaca
     7741 cccggaatac ctgctcaaaa tgcatagcct ggcgcttatc tactacataa atatagcggt
     7801 ctggtttaaa atcctgttcc ctctcaacga tcgtggcaag atcagaggta gcataaaggg
     7861 aagctccgtc tgatttacgg atcagacagg gaggaatctc cttagtatcg ccctcttggg
     7921 ccacatccac aaccagggcg ccctggcttt cataggccag tcctttgtca atgagcatct
     7981 gcaccatatg gtcaatatat ggctctgcgt cgctttcgcc cttccagaga tcgaaatcta
     8041 catttaaatt agagtaattc ttcttaaggt ctgccacaga tacagccaga atgtgatccc
     8101 agatggctct gaagggctta tatcccttct gaagctttaa ggtcgcctga tgagctctct
     8161 ccttaaacgc ttcatccacc tttgatttgg cgctggctgc cggataaatc tcctccagct
     8221 cactgatggt aaagggagcc tctgtgggat actcacccgt ataggattca tcaaagtata
     8281 ccagttccgg ttttctgtcc cttaattctt cgatgatcag acccatctgc agtccccagt
     8341 cgcccagatg aacatcaccg atcatctcat atcccataaa acggcccatg cgcttgatgc
     8401 tctctccgat aatagcagaa cgcagatggc ccacatgaag gggctttgcc acattggcgc
     8461 cgccgtaatc cacgatgatc gtttccttct tttcaggttc ttccagtccc agcttgtccg
     8521 cctctgccat acccttcata taatctgcca gaaacgcctc tgacagcttc agattgataa
     8581 aaccaggcat cacagccact gcttcgccaa acatgggctg ctccctttct gctgtcaatt
     8641 tttccactac cgcatttgca atatcaatcg gcttggtttt atacgccttg gctgctgcca
     8701 tggctccgtt gcactggtac tcgcacagat ccggccggtt ggacaaaacc accttggcaa
     8761 atttatcctc atagccgcag gctgtaaagg ccgccttaag cttctgctcc atcagatcca
     8821 gaattttctt cactccacgt ttctcctttt ctgcacttct tatagatgca aaactgcccc
     8881 ggcagtgaag ccggagcaat cctacatttt ctcacataca aatgaattat acacgggaca
     8941 gatactcgcc ggttcttgta tcgatcttga tcctgtcgcc gatctctacg aacagcggta
     9001 cgcttacaac agcgccggtc tctacagttg caggcttgct tgcaccggtt gctgtatcgc
     9061 ccttaaagcc cggctctgta tctgtgatct ccagctctac aaaaagagga ggctctacag
     9121 agaatacgtt gccgttgtag gagcagacct tgcagatctc gttttcctta acgaacttta
     9181 acgcatcgcc gatcagatcc tggtttaatg ccagctgctc gtagttctct gtgttcataa
     9241 agttgtacag atcgccgtct gcatacagat actgcatatc tacacggtca attcttgctg
     9301 ccgggaattt ctcagttgga cggaaggtgc gctcaaccac accgccgttg atcacatttt
     9361 ttaacttggt acgtacgaaa gcagcgccct tgcctggttt aacgtgctgg aactcaagga
     9421 tctgcactac ctgtccttcg atctccagtg taattccatt tctaaaatcg cctgctgata
     9481 tcataaagat attcctcctt aacgttcttt gtacaggacg cttttctctc cagcgcccgc
     9541 cagggcatga cctgcctttg gcggtcccct gtatatctaa tcttttatat tttatactat
     9601 gtgtccatgt ttttcaagtt ctttttacgg ctttttctgt aaaaataaag cacaggccgt
     9661 tccagatacc gtttgagcac gatctgtatc tcctctttag ggtcaatggg ctttacaaga
     9721 atgcttttaa gtcctgcccg gtttgctcca tatacgtccg taaacagctg gtctcccaca
     9781 aagagagttt ccttcggccc tgtccccata caggccatgg catttttata ccctctcttt
     9841 gagggctttc ctcccttatg gatataagga cttttcacct gcgacgcaaa aggctttact
     9901 ctggcttctt tattattaga aagaagacag gttttaaatc ccagcttttt taaatgttca
     9961 aacaaagcaa tggctctctc atcggcaggt gcgccatgag gcaccagcgt attgtcaata
    10021 tcaaagatca cgcccttaat ccccctgtcc ttccaggttt caaaggggat atcatatgtg
    10081 gagtcccaaa tttcctgagg gtaaaatggt tcaaacataa tccttttgcc tttctcctat
    10141 tttttgagaa gttttcccag ctcctccata aaggtgctca cgtccttaaa ctctctgtac
    10201 acggaagcaa aacgcacata tgcaacctcg tccacctgct taagcttttc cataaccagt
    10261 tcaccgatca ccgcagtaga tatctctctc tcctcccgat taaaaatctg attctcaatc
    10321 tcatcgatca taactccgat ctgctgggga gatacggggc gcttatggca ggagtggatg
    10381 ataccggctt caatcttgga gcggtcatat gcctctctgg tattatcctt tttaatcacc
    10441 ataaggggca tggtttccag tttttcatag gttgtaaaac gcttcccgca gcgctcgcac
    10501 tggcggcgtc tgcggatgga actgttatcg tctgcgggac gggaatcaat tactttcgta
    10561 tctgcttcat tacaataggg gcatttcata tggcttgcac ttcccttctt tatataaaat
    10621 gatgacaatt tatgtcttta tttgcattta ttttaaccga aacacttctc aagtgcaaga
    10681 actttgtaac cagaccaatg aaaatcattg gatggatcgg aatttttttg ctataataaa
    10741 aaaacaaact atactgcagt gaaaggcgga tcaaaatgaa ttgtgtatat gtaagaaatc
    10801 tgcgtctggg agaaggcact cccaaggtct gtattcctct tgtgggagct tcggatgaag
    10861 ctctttttga ggagatcaaa gcgctctctt cctatccggc ggatctggtg gaatggcgga
    10921 cagactacat gaatgatatt tttaatgaag ggcggctgac tggtcttctt ccccgtctca
    10981 gaaaacattt gggaaatacg cctcttattt tcaccttccg taccacaaaa gaaggcggac
    11041 agcgccaggc ctcgcctcag caatataaaa cactggctat ggaggcaatc gcctccggct
    11101 gtatcgatct gatcgatatc gaacttttct ctgatccgga cagcattaca gagcttacag
    11161 ctcttgcaaa agaaaaagga gtaaaaacca ttttttccag tcatgacttt tactccaccc
    11221 cctcctgcgg agaactcctg tcacggctgc gccacatgga aaagctggga gccgatattg
    11281 caaaaatcgc cgtgatgccc cggtgtccgg aagatgtgct tacgctgctt acagctaccg
    11341 ttacagcccg acgccagctc acctg
//
LOCUS       DBMDUH010000083        11302 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_2892_length_11302_cov_8.245782, whole genome shotgun sequence.
ACCESSION   DBMDUH010000083 DBMDUH010000000
VERSION     DBMDUH010000083.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 11302)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11302)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11302
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2892_length_11302_cov_8.245782"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="ACZPCG_08135"
     CDS             <1..882
                     /locus_tag="ACZPCG_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006776458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HIR1285066.1"
                     /translation="WSMIDKITPRPADSVCQELEKAGIEDMAPVITSKKTYIAPFVNA
                     EGPQYLVIEDKFPNGRPALEKAGVYMTDRDTVNKVERMKVTTCLNPLHTALAVYGCVL
                     GYDLIADEMKDEQLNKLVHQIGPVEGMPVVTDPGILSPVDFVNEVIQVRIPNPFMPDT
                     PQRIATDTSQKVGIRYGETIKSYVAQYGDAKKLTAIPLAIAGWCRYLLAVDDEGKAFE
                     LSADPMVPELTKQLEGIVVGKPETYTGQLKSILSNANIFGIDLYEAGIGERIEEMFLE
                     EIAGPGAVRNVLKKYLA"
     gene            1034..2023
                     /gene="holB"
                     /locus_tag="ACZPCG_08140"
     CDS             1034..2023
                     /gene="holB"
                     /locus_tag="ACZPCG_08140"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HIR1285067.1"
                     /translation="MLVFKDILGHEQIKEHFKNAVETGKVSHAYILSGEAGMGRKSLA
                     NAFALSLLCEKGKPEPCMQCHACRQVLSGNHPDLIYVTHEKPASIGVDEIREQINNTI
                     MIRPYSSYYKIYIVDEAEKMTVQAQNALLKTIEEPPSYAVILLLTTNQDAFLPTILSR
                     CVQLKLKPLRDSVVKEYLIQSLKVEEAQAEIYAAFARGNLGKAIHLADSEDFKIMYEE
                     ILHLLKHLKDMDISELLNYIRKLKDDNLDLHDCLDFMQMWYRDILMYKTTKDINLLIF
                     KDEFSTVKQVAALSGYEGLERILAAIDKARVRLDANVNMELVMELMLLTMKEN"
     gene            2050..2985
                     /locus_tag="ACZPCG_08145"
     CDS             2050..2985
                     /locus_tag="ACZPCG_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004614425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSP1 domain-containing protein"
                     /protein_id="HIR1285068.1"
                     /translation="MLTVIGVRFRTAGKIYYFDPAGRDIKTGDHVIVETARGIEYGYV
                     VLGNREVEEGKVVPPLKPVIRMATDEDRAVETRNKEKEKEAFKTCQEKIRKHKLDMKL
                     IDAEYTFDNNKVLFYFTADGRIDFRELVKDLASVFKTRIELRQVGVRDETKIMGGIGI
                     CGRPLCCHSYLSEFIPVSIKMAKEQNLSLNPTKISGVCGRLMCCLKNEEETYEVLNSR
                     LPAIGDYVTTADGLKGEVHSVNVLRQLVKVVVVVDRDEKEIREYKVEELKFKPRKKKG
                     KNGERPSKDSANTDTELKKLEALEKKEGKSKLNDK"
     gene            2975..3745
                     /locus_tag="ACZPCG_08150"
     CDS             2975..3745
                     /locus_tag="ACZPCG_08150"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HIR1285069.1"
                     /translation="MINKEHTWTETGGIRLRDDERIDDLQRNHYGIIQRKGAFCFGMD
                     AVLLSGFAAVKKGERALDLGTGTGIIPILLSAKTEGEHFTGLEIQEESADMARRSVSY
                     NHLEERVDIVTGDIKEAGTLFARASFDVVTSNPPYMNDAHGIKNPGDAKAIARHEVLC
                     TLEDVVREGTCLLRPGGRFYMVHRPRRLIEIIQTMKKHGLEPKRMKFVHPYRDREANM
                     VLIEAVRGGGALLKAEAPVIVFDENGEYSEEIRTTYGY"
     gene            3760..4605
                     /gene="rsmI"
                     /locus_tag="ACZPCG_08155"
     CDS             3760..4605
                     /gene="rsmI"
                     /locus_tag="ACZPCG_08155"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008726627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HIR1285070.1"
                     /translation="MAGILYLCATPIGNLEDITYRVVRTLEEADLIAAEDTRHSIKLL
                     NHLGIKTPMTSYHEYNKVDKAKYLVEKLKEGVNIALITDAGTPGISDPGEELVRQCYE
                     AGIAVTSLPGPAACITALTMSGLPTRRFCFEAFLPAEKSDKKERQRILEELKNETRTI
                     ILYEAPHHLKSTLKDLYEAMGDRDITICRELTKRFESAWRTSFTEALAVYEEEEPKGE
                     CVIVIRGRSVQEIQEEVRQSWEALTLEEHMEHYLAQGMDKKGAMKAVAKDRGISKRDV
                     YQGLL"
     gene            4639..5430
                     /locus_tag="ACZPCG_08160"
     CDS             4639..5430
                     /locus_tag="ACZPCG_08160"
                     /inference="COORDINATES: protein motif:HMM:NF018448.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HIR1285071.1"
                     /translation="MSREMIFSENEYDRMNYLMTAIMDAGELLAMHGAEVSRVEDTIT
                     RLCMAYGFVRADVFTITSSIIVTAMLSDGRTMTQTRRIRTRTTDLEKVAKVNELSRRI
                     CADPAGLNELKAGLDAIKNGKRISVGLELAMHMMIASSLSVFFGGTLMDGIAAALSGA
                     ILYATLHGSAVLRLNTIIQNMFCSAVTAISVLLLVRAGIGVQPDKIMIGDIMLVIPGL
                     QLTNSLRDMINGDMISGLLNMSEALLKAVAVAIGFAGVIILGGAI"
     gene            5427..5909
                     /locus_tag="ACZPCG_08165"
     CDS             5427..5909
                     /locus_tag="ACZPCG_08165"
                     /inference="COORDINATES: protein motif:HMM:NF024230.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HIR1285072.1"
                     /translation="MSFSVIQTIMGMIGSVGFAVLFGVRGRKLAVIALGSGAGWAVYL
                     VCRAMGCSIFTGLLAASLFVAALSEVLARMLRTPVILLLVPMLIPEVPGEALYYTMYY
                     LVQGYQEEFGTYSGLVLTEAGAIALGIILASHMARFFISLKSHLRSRGNKKRESNRRI
                     "
     gene            5915..6268
                     /locus_tag="ACZPCG_08170"
     CDS             5915..6268
                     /locus_tag="ACZPCG_08170"
                     /inference="COORDINATES: protein motif:HMM:NF019584.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HIR1285073.1"
                     /translation="MKYVYASRSGNVEELVHVLGLEAMKIKDGTETMDQDFILFTYTD
                     GRGVVPPVVTAFLKENHSYLKGVIVSGNMQRHGDTFCGAGRIISEMYGVPVIAQVDGV
                     GDSYDHRAIRGKLSL"
     gene            complement(6352..6618)
                     /locus_tag="ACZPCG_08175"
     CDS             complement(6352..6618)
                     /locus_tag="ACZPCG_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0001558 - regulation of cell growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HIR1285074.1"
                     /translation="MKTTGVIRKLDELGRITLPIELRRSLDLDVKDGLEITVQDDCII
                     LKKAESADIFTGSTEDLLEYEGKKVSRSSILALAKLAGLNVSEA"
     gene            6937..7899
                     /locus_tag="ACZPCG_08180"
     CDS             6937..7899
                     /locus_tag="ACZPCG_08180"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008726622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HIR1285075.1"
                     /translation="MTTRIIGTGSYVPEQIVTNDDLSKIVDTNDEWIRSRTGIGERRI
                     ATEDSTSDMAAKAAARAIEQAGIAPEEIDLILLATSSPDSCFPNGACEVQAKIGAIHA
                     ACYDISAACTGFVFALSTAHAFISSGIYRTALVIGADVLSKLVDWTDRGTCVLFGDGA
                     GAAVVSADETGVLGLNMHSNGAKGEVLTCGSRSNGNFLLGRKPELGYMAMDGQEVFKF
                     AVKKVPECISQVLEDTGYITGDIDHFIIHQANYRIIESIAKRLKVDIEKFPVNMEHYG
                     NTSGASVPILLDELNQAGKLKRGERIVLSGFGAGLTWGALLLEW"
     gene            8040..8273
                     /gene="acpP"
                     /locus_tag="ACZPCG_08185"
     CDS             8040..8273
                     /gene="acpP"
                     /locus_tag="ACZPCG_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008397736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR1285076.1"
                     /translation="MLEKMKEIIAEQLSVDAESVTEASSFKEDLGADSLDLFELVMAL
                     EDEYSVEIPAEELEKMSTVGDVMEYLKNHGVEA"
     gene            8371..9297
                     /gene="fabK"
                     /locus_tag="ACZPCG_08190"
     CDS             8371..9297
                     /gene="fabK"
                     /locus_tag="ACZPCG_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HIR1285077.1"
                     /translation="MKTRITEMLGIEYPIIQGGMAWVAEHNLAAGVSAAGGLGLIGGA
                     NAPGEVVREEIRKARALTDRPIGVNVMLLSPHADDVARVVVEEGIKVVTTGAGNPEKY
                     MQMWKEAGVKVIPVVASVALARRMEKYGADAVIAEGTESGGHIGEETTMTLVPQVVDA
                     VSIPVIAAGGIGDGRGIAAAFMLGAEAVQMGTRFVVAKESTVHENYKQRIIKAKDIDS
                     VVTGRTHGHPVRGLRNQMTREYTKLEQEGKSFEELEYLTLGALRKAVQEGDVMNGTVM
                     AGQIAGMISKEQTCKEMIDEMMEQAKKLLNRE"
     gene            9301..10227
                     /gene="fabD"
                     /locus_tag="ACZPCG_08195"
     CDS             9301..10227
                     /gene="fabD"
                     /locus_tag="ACZPCG_08195"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HIR1285078.1"
                     /translation="MSRIAFIFPGQGAQTCGMGKDFYEQTETGRKVFDRASDLLGFSV
                     PELCFEPNDRLDITEYTQAAMVTTSIAMMKVLEERGIHPDVAAGLSLGEYCALAAAGV
                     MSEDDAIRTVRQRGILMQEEVPAGEGAMAAVLALDASVIEEITSSMEGVWIANYNCPG
                     QIVISGKKAAVEEACERLKATGAKRAILLNVSGPFHSGMLTGAGEKLGEVLSGVEVNA
                     PRIPYVANVTAEYVTEAAPVKELLIRQVSSSVKWQQSVEAMLADGVDTFIEIGPGRTL
                     TGFMRKINRSVKSLNVEKLEDIDKVIEALKEN"
     gene            10230..10970
                     /gene="fabG"
                     /locus_tag="ACZPCG_08200"
     CDS             10230..10970
                     /gene="fabG"
                     /locus_tag="ACZPCG_08200"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008726615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HIR1285079.1"
                     /translation="MLEGKIALVTGASRGIGRQIAKTLAAKGATVIVNYNGSAAKAEE
                     AVQEIREAGGIAEAVQCNVSDFNSSKEMLDGIVAKYGRLDILVNNAGITRDNLVMKMS
                     EEDFDAVIQTNLKGVFNCIHHISRQMLKQRSGRIINISSVSGVLGNAGQANYCAAKAG
                     VIGITKSVAREMASRGITVNAIAPGFIRTEMTDVLKDDIKKALMEQIPMKNFGEVEDI
                     ANTVAFLASEDARYITGQVISVDGGMAM"
     gene            11001..>11302
                     /locus_tag="ACZPCG_08205"
     CDS             11001..>11302
                     /locus_tag="ACZPCG_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008726614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HIR1285080.1"
                     /translation="MKTRVVVTGMGAITPIGNDVESFWQALKDKTLGIGPITYFDTAD
                     FKCKLAAEVKDFDAKAYMDPKTARRMEPFSQFAVAAAKQALDQSGIDMEKEDPFRVG"
BASE COUNT         3193 a         2244 c         3198 g         2667 t
ORIGIN      
        1 tggtctatga ttgataagat taccccaaga cctgctgatt ctgtatgcca ggagctggaa
       61 aaggccggaa ttgaggatat ggctccggtt attacttcca aaaagaccta cattgctcct
      121 tttgtcaatg cagagggacc ccagtatctg gttatcgagg ataaattccc caacggaaga
      181 cctgctcttg agaaggccgg cgtatatatg acagaccgtg atacggtaaa taaggtagag
      241 cgcatgaagg taacaacctg tctgaatccg ctccataccg ctcttgccgt ttatggctgc
      301 gtactgggat atgacctgat tgcagatgag atgaaggatg agcagctgaa taagcttgta
      361 catcagatcg gtcctgtaga gggtatgccg gttgttacag atccgggcat tctttctcct
      421 gtagattttg taaatgaagt aattcaggta agaattccga atccatttat gcctgataca
      481 cctcagcgta tcgcaactga tacatcccag aaggtaggta tccgctatgg cgagacaatc
      541 aagtcttatg tagcgcagta cggcgatgcg aagaagctga ctgcaattcc tctggcaatc
      601 gcaggctggt gccgttacct tctggcagta gatgatgaag gcaaggcatt tgagctgtct
      661 gctgatccta tggttcctga actgacaaag cagctggagg gaattgtggt aggaaagccg
      721 gagacctata ccggtcagtt aaagagcatt ttatcaaacg caaatatttt cggaattgat
      781 ctttatgaag caggtatcgg agagcggatc gaagagatgt tcttagaaga aattgcaggc
      841 ccgggagctg tcagaaacgt tctgaagaag tatctggcat agtttaaggt taataaaaac
      901 agtcggaaag ccggtacagg ccgccaataa gcgcggcctg taccggcttt tgccatatga
      961 aaaggctata catcaaaatt aacttatgtt atactggtta aagcataaaa agcaaccaaa
     1021 gggggtatgg cagatgctgg tttttaagga tattctggga catgagcaga tcaaggagca
     1081 ttttaaaaat gcagtggaaa caggcaaggt ctctcatgcg tatatactca gcggcgaagc
     1141 gggaatgggg cgaaaatccc ttgcaaatgc ctttgcactg agccttttgt gtgaaaaggg
     1201 aaagccggag ccctgtatgc agtgtcatgc ctgcagacag gtgctttcgg gtaatcaccc
     1261 ggatctgatt tatgtgactc atgaaaagcc agccagtatt ggcgtggatg agatccggga
     1321 gcagattaat aataccatta tgatccggcc ctacagcagc tattataaga tctacatcgt
     1381 agatgaagcg gaaaaaatga cggttcaggc tcagaatgcc cttcttaaga ctattgaaga
     1441 gccgccctct tatgcggtaa ttctgcttct taccacgaat caggacgcct ttcttcccac
     1501 tattctttcc agatgtgtgc agttgaagtt aaagcccctg cgtgattcgg tggtaaaaga
     1561 atatctgatc cagtcgctga aggtggaaga agcacaggct gagatctatg ctgcatttgc
     1621 caggggaaat ctgggaaagg cgattcatct ggcagattcg gaggatttta agattatgta
     1681 tgaggaaatt cttcatctct taaagcatct gaaggatatg gatatctctg agctgttaaa
     1741 ttatatccgt aaattaaagg atgataatct ggatcttcat gactgcctgg attttatgca
     1801 gatgtggtac agggatattc tgatgtataa gacaactaag gacattaacc ttttgatctt
     1861 taaggatgaa ttttctactg taaagcaggt tgcggcttta agcggatatg agggtctgga
     1921 acggatactg gcagccattg acaaggccag agtacgtctg gatgccaatg taaatatgga
     1981 gcttgtgatg gaactgatgc tccttaccat gaaagagaat tgagagtaag cgtaaataca
     2041 ggaggaatga tgttgacagt tatcggcgtt cgttttcgga ctgcgggcaa aatctactat
     2101 tttgaccctg cgggccgtga tataaagacc ggcgatcatg tgatcgttga aacagccaga
     2161 gggattgagt acggctatgt agtgcttgga aaccgtgagg tggaggaagg aaaggttgtg
     2221 cctccgttaa aaccggtgat ccggatggct accgatgagg atcgggcagt ggaaacccgg
     2281 aacaaggaaa aggaaaaaga ggcatttaag acctgtcagg aaaagatccg caagcacaag
     2341 ctggatatga agctgattga tgcggaatat acctttgata ataataaggt gctgttttac
     2401 tttacagcag acggaaggat tgatttcaga gaacttgtaa aggatcttgc aagcgtattt
     2461 aagacaagga ttgagctgcg ccaggtaggc gtgagggatg aaacaaagat catgggcgga
     2521 atcggaatct gcggaaggcc cctgtgctgc cattcttatc tgtccgagtt tattccggtg
     2581 tccatcaaga tggcaaagga acagaatctt tccttaaatc ccaccaagat ttccggcgtc
     2641 tgcggccgcc ttatgtgctg cctgaaaaat gaggaagaga catacgaggt attaaacagc
     2701 aggctcccgg ccattggaga ctatgtgaca acggcagacg gcttaaaggg agaggtacac
     2761 agcgtcaatg tactgcgtca gcttgtaaag gttgtggtag tggttgaccg ggacgagaag
     2821 gaaatccgcg agtacaaggt agaggagctg aagtttaagc ccagaaagaa aaagggcaaa
     2881 aacggggagc ggccgtctaa agacagcgca aatacggata cagagctgaa aaaactggaa
     2941 gcactggaga aaaaggaagg aaagtcaaag ctcaatgata aataaggagc atacctggac
     3001 tgaaacaggc gggatccgtc tgcgggatga cgagcggatt gatgatctgc agaggaatca
     3061 ttatggaatt atccagagaa aaggcgcatt ctgttttgga atggatgcgg tgcttctttc
     3121 tggctttgcg gcggtaaaga agggggaacg ggctttggac ctgggaacgg gaacaggaat
     3181 cattcctatt cttttaagcg ccaaaacaga gggagaacat ttcacaggac tggaaatcca
     3241 ggaggaatcc gctgatatgg cccgcagaag cgtgtcatac aaccatctgg aggaacgggt
     3301 ggatattgta acaggtgata taaaggaggc cgggacgctc tttgcccggg cttcttttga
     3361 tgtggtgacc agcaatccgc cctatatgaa tgacgctcac ggcatcaaga accctggaga
     3421 tgccaaggcc attgcccgcc atgaggtgct gtgtaccctg gaggatgtgg tgcgggaggg
     3481 aacctgcctg ctgcgtcctg ggggacgttt ttacatggta cacaggccaa gacggctgat
     3541 tgagatcatt caaaccatga aaaaacacgg tctggagccc aaacggatga agtttgtcca
     3601 cccgtacagg gacagagagg ccaatatggt gctgattgag gcagtccgcg gaggcggcgc
     3661 tctcttaaag gcagaagcgc cggtgatcgt gtttgacgaa aacggagaat attccgaaga
     3721 aatacggact acctacggat actgaaagga gctgtgatca tggcaggaat tttatatttg
     3781 tgtgcaacgc ctattggaaa tctggaggat atcacctacc gggttgtgcg gacgctggaa
     3841 gaggcggatc tgatcgcggc agaggatacc cgccacagca ttaagctgtt aaaccatctg
     3901 gggataaaga cacctatgac cagctatcac gaatacaata aggtagacaa ggcaaaatat
     3961 ctggtggaaa agctgaagga aggggtaaat attgccctga ttaccgatgc gggaacaccg
     4021 ggtatttccg atccgggaga agagctggta cgccagtgct atgaggccgg gattgcagtg
     4081 acctcccttc cgggacctgc tgcctgtatt acggctctta ccatgtcggg gcttcccacc
     4141 cgccgtttct gctttgaggc ttttctgcct gcggaaaaga gcgacaaaaa ggagcgccag
     4201 aggattttgg aagaactgaa aaatgaaacc aggaccatta tcctgtatga agcgccccac
     4261 catttaaaga gtacccttaa ggatttgtac gaggcaatgg gagacaggga tatcaccatc
     4321 tgccgagagc tgacaaagcg gtttgaaagt gcctggagaa cgtcatttac ggaggctttg
     4381 gccgtatatg aggaggaaga acctaagggt gagtgcgtaa tcgttatccg ggggcgttct
     4441 gttcaggaaa ttcaggaaga agtaaggcag tcctgggaag ccctgacgct ggaagagcat
     4501 atggagcatt atctggctca ggggatggat aaaaaagggg ccatgaaggc ggttgcaaag
     4561 gaccggggga tatcgaaaag ggacgtgtat caggggctgc tgtaaccgga agcccagctg
     4621 tctgaggatg atataaagat gagcagagaa atgattttca gtgaaaatga gtatgaccgg
     4681 atgaattatc ttatgacagc gattatggat gcaggagaac tgcttgccat gcacggtgca
     4741 gaggtaagcc gggtagagga tacgattacc agactgtgca tggcgtacgg ctttgttcgg
     4801 gcagatgtat ttaccattac ctccagtatc attgtcacag ccatgctttc agatggaagg
     4861 accatgaccc agacaaggcg catccggacg agaactacag atcttgaaaa agtggcaaag
     4921 gtaaatgagt tgtcccgcag aatctgcgcc gatccggcag gactgaatga actgaaggca
     4981 ggactggacg cgataaaaaa cggaaaaagg atttccgtag gcctggagct ggcaatgcac
     5041 atgatgattg cttcctccct gtctgtcttt ttcggaggga ctctgatgga cggcatagcg
     5101 gcagctcttt caggcgccat tttgtatgct acccttcacg gaagtgcagt tttaaggctg
     5161 aataccatta ttcagaatat gttctgcagt gcagtaacag caatatcggt acttcttttg
     5221 gtaagggcgg gaatcggtgt gcagccggat aaaattatga ttggcgatat tatgctggta
     5281 atccccggac ttcagctgac caactctctg agagatatga taaacggaga tatgatcagt
     5341 gggctgctga atatgagtga ggcgctttta aaagcagttg cagtagccat aggctttgcc
     5401 ggtgttatca ttttgggagg ggcaatatga gcttttcggt aattcagacc attatgggaa
     5461 tgatcggttc cgttggtttt gcagtccttt ttggcgtccg gggcagaaag ctggctgtga
     5521 tcgcccttgg aagcggcgcc ggctgggcgg tataccttgt atgcagggcg atgggatgca
     5581 gtatatttac cggtcttctg gcagccagcc tttttgtggc ggccttaagt gaggttcttg
     5641 ccagaatgct gcgcacgccg gtcattcttt tgcttgtgcc catgctgatt ccggaggttc
     5701 cgggagaagc cctttactat accatgtatt atctggtgca gggatatcag gaagaatttg
     5761 gaacttactc agggctggtg ctcacagagg caggagccat tgctctgggg atcatacttg
     5821 cttcccatat ggcaagattt tttataagcc tgaagagtca tttgcgaagc aggggaaata
     5881 aaaaaagaga atcgaacagg aggatttaga atttatgaaa tacgtatatg cgtcacgcag
     5941 tggaaatgta gaggaactgg ttcacgttct tggattagag gcgatgaaga taaaggatgg
     6001 tacggaaacc atggatcagg acttcattct ttttacctat acggatggca gaggcgttgt
     6061 gccgcctgtt gtgacagcgt tcttaaaaga gaatcattct tatttaaagg gcgtgatcgt
     6121 atccggaaat atgcagcgtc acggtgatac cttctgtgga gcgggcagga tcatttcaga
     6181 aatgtatggt gttcctgtga tcgctcaggt agatggagtg ggagactcct acgaccatag
     6241 ggcgatcagg gggaagcttt ccctgtaatt aacaaaatat ccccctgcat atcgcattca
     6301 gcgtgcacag ggggatattt ttatatagtt ttgaaagact gcagctttct gtcaggcttc
     6361 tgatacattc agcccggcca gcttcgccag agctaaaatg gagcttcggg aaactttctt
     6421 tccctcgtat tccagcagat cttcggtgct gccggtaaag atatctgcgc tttccgcttt
     6481 tttaaggata atacagtcat cctgaacggt tatctcaagt ccatctttga catccaggtc
     6541 aagacttctc cttaattcaa tagggagcgt gattctgccc agctcatcca gcttgcggat
     6601 tactcctgtg gttttcatca tatgacctcc tcgctttaca ggtataattt atagacttct
     6661 atgattttaa agtaacaaaa tacagaaata aagtcaataa atggaaaagt gtaaattggt
     6721 aaattgtggt ataatatagt aaattttcgg aatattcttt ttgaagaaat ggggtaaaat
     6781 gcgacgattt aaggtatgtc attggagaaa attgcttgac aaaaaacacg caatcctgta
     6841 gtattacttt gagattcaaa gtattttggc aaaggaaggt acagccctca ggcagcggag
     6901 gttttgaacc gctcattcag ggccgggtaa ataaaaatga caaccagaat aataggaacc
     6961 ggctcgtatg tgccggagca gatcgtgaca aacgatgatc tgtcaaagat cgttgacaca
     7021 aatgatgagt ggatccgcag ccgtacaggc ataggggaaa gacggatcgc cacagaggac
     7081 agtacatctg atatggcggc aaaggcggca gccagagcca ttgagcaggc cggaattgct
     7141 ccggaggaga ttgacctgat tctcttagct acttcatcac cggattcctg ctttcccaat
     7201 ggggcctgtg aggtacaggc aaagatcgga gccattcatg cggcctgcta cgatatcagc
     7261 gcagcctgca ccggctttgt ttttgccctc agtacagccc acgcctttat ttcctccgga
     7321 atataccgga ctgcccttgt gatcggagca gatgtgctca gtaagctggt tgactggaca
     7381 gaccgcggaa cctgcgtctt gttcggagac ggagcaggtg cagctgtggt aagcgcagac
     7441 gagacaggcg ttctgggatt aaatatgcat tcaaacggtg caaagggtga agtgcttacc
     7501 tgcggctccc gttccaatgg aaatttcctt ctgggaagaa aaccggagct tggatatatg
     7561 gctatggacg gacaggaggt ctttaagttc gcggtaaaga aggttccgga gtgcatcagc
     7621 caggtactgg aggacacggg atatattacc ggcgatattg accatttcat tatccatcag
     7681 gccaattaca ggattatcga atccattgca aaacgcctga aggttgatat cgaaaagttc
     7741 cctgtgaata tggaacatta tggaaatacg tccggagctt ctgttcctat ccttttagac
     7801 gagctgaacc aggcaggtaa gttaaagcgc ggagagcgga ttgtattatc aggatttgga
     7861 gcaggtctta cctggggagc tctgcttttg gagtggtaag acggtaaaaa atcaccaaaa
     7921 tgcttaaaaa aagataaaaa atgtaaagcc ctgtaaaaag ggcatggaca tttcgtgaat
     7981 ataatggtaa atatttaaat tgtaatataa aacttatatg tcagaaggag acaaagatta
     8041 tgttagagaa gatgaaagaa attattgcag agcagttaag tgtagacgct gagtccgtaa
     8101 cagaggcttc ctcttttaag gaagatctgg gcgctgattc cctggatctc tttgagctgg
     8161 taatggcttt agaggatgag tattctgtag agatcccggc agaggagtta gagaagatgt
     8221 ctacagttgg cgatgttatg gagtacttaa agaaccacgg cgtagaggca taagatcctc
     8281 aaggtcagag tgtaaaattg cataagcaaa tgagagggac ggcgtgtgga tggattgaag
     8341 cacgctgttt cctctattgg aaggaagaca atgaaaacaa gaattacaga aatgttagga
     8401 attgaatacc ccattatcca gggcggtatg gcctgggtgg cagagcataa cctggcagca
     8461 ggcgtgtccg cagcaggcgg cctggggctg atcggcgggg caaacgcacc gggagaggtg
     8521 gttcgtgagg aaatccgcaa ggcaagagcg ctcacagacc gccctatcgg cgttaatgta
     8581 atgcttttaa gtcctcatgc agatgacgtg gcaagagttg tagtggagga aggcattaag
     8641 gtagtcacca ccggagccgg aaatcctgag aaatatatgc agatgtggaa ggaagcaggc
     8701 gttaaggtaa tcccagtggt agcttctgtg gcactggccc gccgtatgga aaaatatggg
     8761 gctgatgcag taatcgcaga gggaacagag tccggcggcc acatcggtga ggagactacc
     8821 atgaccctgg ttccccaggt agtggatgca gtttccatcc ctgtcattgc ggcaggcggt
     8881 attggagacg gaagaggcat tgccgcggca tttatgctgg gagcagaggc ggttcagatg
     8941 ggaacccgct ttgtagtggc aaaggaatcg accgtacatg aaaactacaa gcagcgcatc
     9001 atcaaggcaa aggatattga ctccgtagta accggaagaa cccacggcca tcccgtaaga
     9061 ggtctgagaa atcagatgac aagagagtat acaaagttag agcaggaagg aaagtccttt
     9121 gaggagctgg agtacctgac gttaggagct ttgcgcaagg ccgtacagga aggcgatgta
     9181 atgaacggta ctgtaatggc aggccagatc gccggaatga tctccaaaga gcagacctgt
     9241 aaggaaatga tcgacgagat gatggagcag gcaaagaaac ttttaaacag ggagtaaaaa
     9301 atgagcagga tcgcatttat tttcccaggt cagggcgcac agacctgcgg tatgggaaag
     9361 gatttttatg agcagacaga gactggaaga aaggtatttg acagagcctc agatctttta
     9421 ggcttttctg ttcccgagct gtgctttgag cctaatgacc gtctggatat taccgaatat
     9481 acccaggcag ctatggtgac aaccagtatt gccatgatga aggtgctgga ggaaagaggg
     9541 atccatccgg atgtggcggc aggcttaagc ctgggagaat actgcgctct ggcagcagca
     9601 ggcgtgatga gcgaggatga cgccatccgc acagtgcgcc agagaggaat cctgatgcag
     9661 gaggaggttc cggcaggaga aggcgctatg gcggctgttc tggcattgga tgcctctgtg
     9721 atcgaggaaa tcacttcttc tatggaaggc gtatggattg ccaactacaa ctgtccgggc
     9781 cagatcgtta tttctggtaa gaaggctgcg gtagaggagg cctgcgagcg cttaaaggca
     9841 acaggagcca agagagccat tttactgaat gtaagcggtc cctttcactc cggtatgctc
     9901 acaggagccg gagaaaagct gggagaggta ctttcaggag tagaggtaaa tgctcccagg
     9961 atcccgtatg ttgccaacgt aaccgcagag tatgtgacag aggcagctcc ggtaaaggag
    10021 cttctgatcc gtcaggtatc ttcttctgta aaatggcagc agagcgtaga ggctatgctg
    10081 gcagatggcg tggatacctt tatcgagatt ggaccgggac gtacgctgac tggctttatg
    10141 cgtaagatca atcgcagtgt aaagagcctg aatgtagaaa aattagaaga tattgacaaa
    10201 gtaatcgaag ctttaaagga gaactagata tgctggaagg aaagatcgca ctggtaacgg
    10261 gcgcaagccg cggaatcggc cgccagattg caaagacact ggctgcaaaa ggcgctacag
    10321 taatcgtaaa ttataacggc tctgctgcaa aggcagagga agcagtacag gagatccggg
    10381 aggccggagg catcgcggaa gcagtgcagt gcaatgtttc cgattttaac agcagcaagg
    10441 agatgctgga cgggatcgtg gcaaagtacg gccgtcttga tatccttgta aataacgcgg
    10501 gcatcacaag ggacaatctg gtgatgaaga tgtccgagga ggactttgac gcagtgatcc
    10561 agaccaactt aaaaggtgta tttaactgca ttcatcatat ctcccgtcag atgttaaagc
    10621 agagatccgg aaggatcatc aatatctcat ccgtatccgg cgttcttgga aatgcaggac
    10681 aggccaatta ctgtgctgca aaggcaggtg tgatcggaat tacaaaaagc gtggcaagag
    10741 agatggcaag ccgcgggatt accgtaaatg ctattgctcc aggctttatc cgcacagaga
    10801 tgacggatgt gttaaaggat gacattaaaa aagctctgat ggagcagatt cccatgaaga
    10861 acttcgggga agtagaagat attgccaata cagtcgcatt cctggcatct gaggacgcca
    10921 gatatatcac aggccaggtc atcagcgtag acggcggaat ggctatgtaa ttcaaaccgg
    10981 aaagagaagg agcggatcat atgaagacaa gagtagtagt aacaggtatg ggagccatca
    11041 cgcccatcgg aaatgatgtg gagagcttct ggcaggcctt aaaggataag acactgggca
    11101 ttggccccat tacctatttt gacacggcag attttaaatg caagctggcg gctgaggtaa
    11161 aggattttga cgctaaggcg tacatggatc caaagactgc tcgccgtatg gagccatttt
    11221 cccagtttgc ggtagctgcg gcaaagcagg ccttagacca gtccggcatt gacatggaaa
    11281 aggaggatcc cttccgtgtg gg
//
LOCUS       DBMDUH010000084        11122 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_2938_length_11122_cov_5.493785, whole genome shotgun sequence.
ACCESSION   DBMDUH010000084 DBMDUH010000000
VERSION     DBMDUH010000084.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 11122)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11122)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11122
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2938_length_11122_cov_5.493785"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..145
                     /locus_tag="ACZPCG_08210"
     CDS             <1..145
                     /locus_tag="ACZPCG_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="large ribosomal subunit protein bL28"
                     /protein_id="HIR1285081.1"
                     /translation="NKVSHSHRRSNKMWKANVKSVKVKVNGGTQRMYVCTSCLRSGLV
                     ERA"
     gene            complement(242..445)
                     /locus_tag="ACZPCG_08215"
     CDS             complement(242..445)
                     /locus_tag="ACZPCG_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285082.1"
                     /translation="MKASGEWEGTLLHKIKRRERFDMKRICGLMLFCFGLGMAVLLFI
                     PETIFTFFFVVCCLVLGYNLFCC"
     gene            complement(511..918)
                     /locus_tag="ACZPCG_08220"
     CDS             complement(511..918)
                     /locus_tag="ACZPCG_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HIR1285083.1"
                     /translation="MAENHFSSMIDTLFKGMDQFVTSKTVVGEPVQAGDAIIVPLIDV
                     TCGMAAGAFSEKTKEKQKGGGGMSAKMSPSAVLVIQNGVTKVVNIKNQDAITKAIDMA
                     PDLINRFMGGKPAVSEETRAAAQDAAAEYDKRD"
     gene            complement(949..1884)
                     /locus_tag="ACZPCG_08225"
     CDS             complement(949..1884)
                     /locus_tag="ACZPCG_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HIR1285084.1"
                     /translation="MVHILLLILKAIGILILVLLGLVLAVVCLVLFVPVCYEAGGSWK
                     DKPAGKGKISWLFGAVSLSAGYDGDESGLTAGVRLFGQKLWEMDKEKEAPETHRPLDE
                     ETLRGTDAERKEPEEKKTTLWEEKRIPAASAENPQKALPKNPQPRPEPEDKRHTEHKK
                     KDVFGRIKVFLEKLKFSFQSFCDKLKNIQNIAEEKKAWLEDEENQASLKLLWKQTGRF
                     LRHVCPRGGKGSVTFGFEEPYLTGQVLSAAALIYPFFEGNLEICPVFDETVFEAEGSF
                     KGRIRAGYLLWLGLGILRDGHTRRMLFGFLKGSAV"
     gene            complement(1895..2215)
                     /locus_tag="ACZPCG_08230"
     CDS             complement(1895..2215)
                     /locus_tag="ACZPCG_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007037312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285085.1"
                     /translation="MRLWDHLYMGQNADRARARILKGLRQGSLLPDTYVITLPENGKH
                     IFDICPVSLLTRRERQGENLLILGIARGYHEAAEVTGRMVDDMYHQTGGFDWKAYMDQ
                     MAGK"
     gene            complement(2256..3563)
                     /locus_tag="ACZPCG_08235"
     CDS             complement(2256..3563)
                     /locus_tag="ACZPCG_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285086.1"
                     /translation="MKAARIRRQLTAAYHCSYIRLALNFYTAYEAAAEKKGFENDRIS
                     GLYDRFCELLDACLKGQSDCGADELRQDVIREMEKVTDYTDAFQAYEYVMNRIEGRFA
                     PKLIGKKPEDTESLTEEILSWFQTAPDPAALRERMQVITGQLPVRFTKQKFFALLEDR
                     LSIYKGLPRSGFEDILYILRSEGLLNRPMAREEAYGTLFDRMKAFEQADLRHLTAQEY
                     RHLASELEEGSREINALSGEILCLIDMVNDLCVILLAQGTAVSDLSCEQNTKEILKGI
                     QTLFASKEWEDIPEEVTDRLPALEGKQEQYFEQWLRDDISLEELQEENGPEAEAAWKA
                     GLLMSGSAFMSLEKPNADEAEVDEVFFREKLDALFKDMGERWKELPKAVVRAAMAKCL
                     SSLPANFQSLDEAREYIQGSLNSCSDEIEREACADLIRSLMEE"
     gene            complement(3579..4838)
                     /locus_tag="ACZPCG_08240"
     CDS             complement(3579..4838)
                     /locus_tag="ACZPCG_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005948679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HIR1285087.1"
                     /translation="MVVELISVGTEILMGNILNSNTQYLAEKCALLGLDQYYQVTVGD
                     NYGRMMESVKRALHRSDIVILTGGLGPTEDDLTKEVCAEAMEMELVEDPHTRSQLEAF
                     FAGNIYKEIPDNNWKMAMVPQGAIVLDNPNGMAPGLILEKNGKTAILLPGPPNELYPL
                     FEGQVFPYLERLQQSVLVSRMVKICGYGESQVEDKLLDLIDGQTNPTIATYAKTAEVH
                     IRITARAADYEEGKALVAPVIREIKSRFGNAVYTTDEKTSLEMAVAALLKEKQLTMAA
                     AESCTGGMVAARMVNVSGVSDVFMQGMVTYSNEAKMRLLGVREETLAAYGAVSEETAV
                     QMAEGGAAAAGTDVCVSITGIAGPEGGTPEKPVGLVYMACCVKGKTVVKRYQFKGNRA
                     KIREQSTVKALDLVRLCVLEYENRSDS"
     gene            complement(4840..5382)
                     /gene="pgsA"
                     /locus_tag="ACZPCG_08245"
     CDS             complement(4840..5382)
                     /gene="pgsA"
                     /locus_tag="ACZPCG_08245"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HIR1285088.1"
                     /translation="MNLPNKLTVLRICMIPFFVAALLYENGADQTMRIIANVIFITAS
                     LTDLLDGKIARKYGLVTNFGKFMDPLADKLLVCSALICLIQLGQLPAWVVIVIISREF
                     IISGFRLVAADNGIVIAASYWGKFKTVFQMTAVVLLIFNIPPLSVLTSAVLWIAVILT
                     VVSLVDYVAKNIKVLTEGGL"
     gene            complement(5366..6742)
                     /gene="rimO"
                     /locus_tag="ACZPCG_08250"
     CDS             complement(5366..6742)
                     /gene="rimO"
                     /locus_tag="ACZPCG_08250"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HIR1285089.1"
                     /translation="MKKIKLFCVSLGCDKNLVDTEKMLGVAGGLGVTFTDDETEADAV
                     LINTCCFIGDAKEESVNTILEMARLKEEGKCRALIVAGCLAQRYKEEITEEIPEVDAI
                     LGTTSYEEIGRVLTELFGEDERDGEGTADSHISVFHSLSELPHTAKKRVVTTGGYYAF
                     LKIAEGCDKRCTYCIIPSLRGPYRSVPMERLLEEARELADNGVRELILVAQETTLYGK
                     DIYGEKSLPKLLHELAKIPGIYWIRIQYCYPEEITDELIEAIKSEEKVCHYLDIPIQH
                     ASDRILRRMGRRTHKEELKERIAKLRREIPDIALRTTLISGFPGETEEDHEELMEFVD
                     EMEFERLGVFAYSAEEDTPAYSYPDQVPQEVKEERRDAIMELQQEIAFEHCEKMVGKV
                     LTVMIEGKVADEPAYVGRTYMDAPNVDGLIFVNADEMLMSGDFVRVKVTGAAEYDLIG
                     EVYHESAQ"
     gene            complement(6845..7129)
                     /gene="rpoZ"
                     /locus_tag="ACZPCG_08255"
     CDS             complement(6845..7129)
                     /gene="rpoZ"
                     /locus_tag="ACZPCG_08255"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016286441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HIR1285090.1"
                     /translation="MLHPSYTDLINVVNSDIEPGEQPVVQSRYSIVIAASKRARQLIG
                     GEEPLVPGTAGKKPLSIAIDELYHQQVKILPEEETTEEEEADAKEAAQNE"
     gene            complement(7219..7851)
                     /gene="gmk"
                     /locus_tag="ACZPCG_08260"
     CDS             complement(7219..7851)
                     /gene="gmk"
                     /locus_tag="ACZPCG_08260"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - GMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HIR1285091.1"
                     /translation="MNQQGILVVVSGFSGAGKGTLMKELLRRYDNYALSISATTRKPR
                     EGEENGREYFFLSEEEFKKLRDEHKLIEYAKYVNHYYGTPKEYVEEKMAQGKDVILEI
                     EIQGALKVKKRYPEALLLFVTPPSAEELKRRLVGRGTESIEVINARLKRAAEEADGME
                     AYDYLLINDEIDTCVEKMHQLIQLQHEKTSYHLDFLGKMREDLYHLDERN"
     gene            complement(7875..8753)
                     /locus_tag="ACZPCG_08265"
     CDS             complement(7875..8753)
                     /locus_tag="ACZPCG_08265"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HIR1285092.1"
                     /translation="MLKSMTGFGRCENATDEYKISVEMKAVNHRYLDLSIKMPKKFNY
                     FEAAIRTLLKKYIQRGKVDIFINYEDYTEGNLCLKYNRALAAEYMEHFKRMEEEFHIP
                     NDIHVSTLARCPEVLTMEQTPDDEEHLWEILSSAMEEAAEKFVASREIEGEHLKHDLL
                     GKLDYMTELVDFIKARSPQIISEYRTRLEEKVKELLNGTAVDEGRIASEVVIYADKIC
                     VDEETVRLRSHIENTKRELLSGESVGRKLDFIAQEMNREANTILSKSNDLELSDKAIA
                     LKTEIEKVREQIQNIE"
     gene            complement(8895..9332)
                     /locus_tag="ACZPCG_08270"
     CDS             complement(8895..9332)
                     /locus_tag="ACZPCG_08270"
                     /inference="COORDINATES: protein motif:HMM:NF024183.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR1285093.1"
                     /translation="MGLFDTIKENTNLVRGESSQSAGKFDQQIEAVEGQIREALIQIG
                     TVYLEKHREDCEPEYGEFINQIKALEEQKETLERNKLAAQGLRMCEHCRQIITLDSVF
                     CNKCGSKLEPLEISSAGGRFCPSCGASLAEGDAFCTACGSKVN"
     gene            complement(9346..10125)
                     /locus_tag="ACZPCG_08275"
     CDS             complement(9346..10125)
                     /locus_tag="ACZPCG_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285094.1"
                     /translation="MRVNTGSTKKAEKKGVSFTWKRPQISIKAAGLLGAVLVLVFAVL
                     LLKPSAGKIDTQAMMEEYFAEHSEYSENENLYRSCFDYMVNDLTEQFFQNIKLSLGKL
                     GENFEDKEVSDISSKLGKVLSESFSRAANMEQLAESVTSRSYFEAEKAKKKDGIVSAA
                     VTVHYLDIPDINRRVIEDYTGLSELVNLVINRKNLWMNLTDMAFGDASLILNGFEEKA
                     ESSTADKTYTGTVEFTYNKEEKRWEISSVDSGLLDAWFGIQ"
     gene            complement(10169..10912)
                     /locus_tag="ACZPCG_08280"
     CDS             complement(10169..10912)
                     /locus_tag="ACZPCG_08280"
                     /inference="COORDINATES: protein motif:HMM:NF012798.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR1285095.1"
                     /translation="MGEGKKYVICEKELEVYRRDMLENERSRATIEKYIRDVRAFCRW
                     NGEKEIDRLRVLEWKEYLGTCYAVSSANSMLAALNGYFDFRGWEELRVKPFRQQKRIY
                     REPEEDLSREEYMRLIEEAQRQGKERLKLVMQTICATGIRVSELAFITAEAVKAGRAE
                     VSCKNKKRIVFLPEKLRRILKEYMKKHRIADGPVFITRGKRALNRSNIWAAMKKLCEK
                     AGVDPKKVFPHNLRHLFAKTFYQEGKVWK"
BASE COUNT         2556 a         3199 c         2232 g         3135 t
ORIGIN      
        1 aaacaaagtg agccattccc atagacggtc caacaagatg tggaaagcaa acgtaaaatc
       61 tgttaaggtt aaagtaaatg gcggaaccca gagaatgtac gtatgtactt cctgcttacg
      121 ttccggcctt gtagaaagag cctaattaaa tgcagagcaa aagctcctga ctgtgatggt
      181 caggagcttt tttctgtctg gatcgtctat ttgatgatac cggttctgca gcttccagaa
      241 atcagcagca aaacagatta tacccaagca caaggcagca taccacaaag aaaaaggtaa
      301 atatggtctc gggtataaat aaaagcaccg ccatcccaag gccgaaacag aacagcatca
      361 gtccgcatat ccttttcata tcaaaacgct cccttctctt tatcttatgc agaagcgttc
      421 cttcccattc ccccgaagct ttcatatgcc ctctccgaga tagcaaaagg ccctgcagac
      481 gcaggaccgt aataatgtca tattcctgtt ttaatcccgt ttgtcatatt cagccgcagc
      541 atcctgagcc gctgcgcggg tttcctccga tacagccggt tttccgccca taaaacggtt
      601 aatcaggtcg ggagccatat cgatcgcctt tgtgatggca tcctggtttt tgatgtttac
      661 cacctttgtg actccgtttt gtatcaccag aacggcgctg ggactcatct tggcgctcat
      721 tccgccgccg cccttctgct tttcctttgt tttttccgaa aatgctcctg ccgccatgcc
      781 gcaggtcaca tcaatcagag ggacgataat ggcatctcct gcctgcaccg gttcgcctac
      841 aactgtcttt gaagtgacaa actgatccat ccccttaaat aatgtatcaa tcatagatga
      901 aaaatgattt tctgccatac cgtctttcct cttccttttc tcaacgcgct agactgcgct
      961 tccctttaaa aaaccaaaca gcatgcgcct ggtatgccca tcacgcagga tcccaagacc
     1021 cagccacaaa agatatcccg cccggatgcg tcccttaaag ctgccttctg cctcaaatac
     1081 cgtttcatca aacaccgggc agatttccag attcccttca aaaaaaggat agataagagc
     1141 tgccgccgac aatacctgtc ccgtaagata aggttcttca aacccaaagg ttacggatcc
     1201 cttccctcct ctgggacata catggcgcag aaaacggccc gtctgcttcc agagaagctt
     1261 aagcgacgcc tgattttctt catcctccag ccaggctttc ttttcttctg caatgttttg
     1321 tatattctta agcttatcac aaaatgactg aaaagaaaac ttaagttttt caagaaaaac
     1381 ctttatgcgg ccgaatacat ccttcttttt atgctctgta tgtcttttat cctcaggctc
     1441 cggtctgggc tgcgggttct ttggcagggc cttttggggg ttctctgccg atgcagccgg
     1501 aatgcgtttc tcttcccaaa gggtagtttt cttttcttcc ggctctttcc tttctgcatc
     1561 tgtacctctg agcgtttcct catccaaagg ccggtgggtt tccggtgctt ctttctcttt
     1621 atccatttcc cagagctttt gtccaaacag ccttactccg gctgtcagtc cgctttcatc
     1681 tccatcatat ccggcgctta aggaaacagc tccgaaaagc caggaaattt tccctttccc
     1741 ggccggtttg tccttccagc ttccgcctgc ctcatagcac accggaacaa agagcaccag
     1801 acaaaccaca gccagaacca ggcccagaag aaccagaatc aggataccga tggcctttaa
     1861 aatcaaaagc agtatgtgta ccaatatccg cacctcattt cccggccatt tgatccatat
     1921 atgccttcca gtcaaaaccg cctgtctgat ggtacatatc gtctaccatc cgtcccgtaa
     1981 cctccgccgc ctcatgatag cctctggcga ttcccagaat cagcaggttt tccccctgcc
     2041 gttctctcct cgtgagcaga gatacaggac agatatcaaa tatatgtttc ccattttccg
     2101 gaagagtaat cacataggta tcaggcaaaa ggctgccctg gcggagtcct ttcaggatcc
     2161 gcgctctggc ccggtctgca ttttgtccca tatacagatg atcccacaaa cgcatcctct
     2221 ctcctccttt ttggcaaaat gacctttttc ccgctttact cttccatcag gctgcggatc
     2281 agatctgcac aggcttcccg ctctatctcg tcgctgcagc tgttcaggct tccctgaata
     2341 tattctctgg cctcatccag agattggaaa tttgcaggca ggctggacag gcattttgcc
     2401 atggcagccc gcactacagc ttttggaagc tccttccagc gttctcccat atccttaaaa
     2461 agcgcatcca gtttttctct gaaaaatact tcgtctacct ctgcctcatc tgcattcggc
     2521 ttttccaagg acataaacgc gctgccagac atcagaagac ctgccttcca ggcagcctct
     2581 gcctccggcc cgttttcctc ctgaagctcc tccagagaga tatcatctct gagccactgc
     2641 tcaaaatact gctcctgctt cccttccagg gccggaagac ggtctgtgac ctcttcgggg
     2701 atgtcctccc actcttttga tgcaaaaagc gtctgtattc cctttaaaat ctcttttgta
     2761 ttctgttcgc aggacagatc agatacagcc gttccctgcg ccagaaggat cacgcacaga
     2821 tcatttacca tatctatcag gcacaggatt tctccgctga gagcattgat ctcccggctt
     2881 ccttcctcca gttcagaagc cagatgacga tactcttgtg cagtaagatg tcttaaatct
     2941 gcctgttcaa acgctttcat ccggtcaaac aatgttccat atgcttcttc ccgcgccatc
     3001 ggccgattta acagcccctc ggaacgcaaa atatacagta tatcttcaaa accggagcgc
     3061 ggaagcccct tgtagatgct cagacgatcc tctaacaggg caaagaattt ctgctttgta
     3121 aaacgtaccg ggagctgtcc tgtaatcacc tgcatccgtt cgcgaagggc tgccggatcg
     3181 ggcgcagttt gaaaccagga caggatttct tccgtaaggc tctctgtatc ctccggcttt
     3241 tttccgatca gctttggtgc aaatcttccc tcaatccgat tcatcacata ttcataggcc
     3301 tggaacgcat ccgtataatc cgtaaccttt tccatctccc ggatcacatc ctggcgcagt
     3361 tcatctgccc cgcagtctga ctgtcctttc aggcaggcgt caaggagttc acagaatcgg
     3421 tcatacagac cggaaatccg atcattttca aagccttttt tctctgccgc cgcctcatag
     3481 gctgtataaa agttcagcgc cagcctgata taggagcaat ggtaggccgc cgtaagctgc
     3541 ctgcgtattc ttgctgcttt cataaaaata attcctcctt atgaatccga acggttttca
     3601 tattccaata cgcacaggcg taccagatcc agagccttta ccgtagactg ttcccgaatc
     3661 ttggccctgt tccctttaaa ctggtaacgt tttaccactg tctttccttt tacgcagcag
     3721 gccatatata caagtcccac cggtttttcc ggcgttccgc cctccggccc ggcaattccc
     3781 gtaatggata cgcacacatc cgtcccggct gccgccgctc cgccctctgc catctgtacg
     3841 gccgtttcct cactgaccgc accgtaagca gcaagggttt cctcccgtac tcccaaaaga
     3901 cgcattttcg cctcattgga atatgtcacc atgccctgca taaatacatc cgatacaccg
     3961 gatacgttta ccatgcgggc agccaccatt ccgccggtac aggactcagc cgctgccatg
     4021 gtaagctgct tttcctttaa aagcgcggca actgccattt ccagactggt tttttcatct
     4081 gtggtgtaaa ccgcattccc aaaccgggac ttgatttccc ggatcacagg agccacaaga
     4141 gccttccctt cttcataatc tgccgctctg gctgtaatac ggatatgaac ctccgctgtc
     4201 tttgcatagg tagcaatggt aggattggtc tggccatcga tcaaatccag cagcttatcc
     4261 tccacctggc tctcgccgta tccgcagatt tttaccatcc gagacactaa aaccgactgc
     4321 tggagccgtt ccagataggg aaatacctgc ccttcaaaca gcgggtacag ctcatttggc
     4381 gggcccggaa gaagaatggc tgttttccca tttttttcca atatcaatcc aggagccata
     4441 ccgttaggat tgtccagtac gatcgccccc tggggaacca tcgccatctt ccagttatta
     4501 tccgggattt ctttataaat attgcctgca aaaaacgcct ccagctggct tcgggtatgg
     4561 ggatcctcca ccaattccat ttccatggcc tctgcacaga cttcttttgt cagatcatcc
     4621 tccgtagggc caagtcctcc tgtgaggatt acgatatccg accggtgaag ggctctcttt
     4681 acgctctcca tcatccggcc gtaattatcg ccgaccgtta cctgataata ctgatccagc
     4741 cccaaaaggg cgcatttttc cgccagatat tgcgtattgc tgtttaaaat attccccatc
     4801 aggatctcag ttcctacgct gatcagctca acgaccattt tacaggcctc cttccgtcag
     4861 aacctttata ttctttgcaa cataatccac cagggatacg accgtcagga tcacggcgat
     4921 ccacaaaaca gcgcttgtca gaacagacag aggcgggatg ttgaagatca gaagcactac
     4981 tgccgtcatc tggaacacgg ttttaaattt gccccagtag ctggctgcga tcacaatgcc
     5041 gttatctgct gccaccaggc ggaagccgct gatgataaac tcacggctga tgataacgat
     5101 cacaacccag gcaggaagct ggcccagctg gatcaggcag atcagggccg agcagactaa
     5161 aagtttatct gccaggggat ccataaattt tccaaaattt gtcacaagtc cgtattttct
     5221 ggcaatcttt ccgtctaaaa gatccgtcag gctggctgta ataaaaatca cattggcaat
     5281 gatccgcatg gtctgatcgg cgccattttc ataaagaagg gcggccacaa aaaacgggat
     5341 catacagata cgaagaacgg taagcttatt gggcagattc atgatacacc tctcctatca
     5401 ggtcatactc ggcggctcct gtgaccttta cccttacaaa gtcgccggac atcagcatct
     5461 catcggcatt tacaaaaatc agtccgtcca cattgggcgc atccatgtag gtgcggccta
     5521 cataggccgg ttcatctgct acctttccct cgatcatgac agtcaggact tttcccacca
     5581 ttttttcaca gtgttcaaac gcaatctcct gctgaagctc catgatggca tcccggcgct
     5641 cctcctttac ctcctggggc acctgatcgg gataggagta cgcaggggta tcttcctctg
     5701 ccgaataggc aaacactcca agacgctcaa actccatctc gtccacaaac tccatcagct
     5761 cctcatgatc ctcctctgtc tctcccggaa aaccggagat cagagtagta cgcagggcaa
     5821 tatccgggat ctctctgcgc agttttgcaa tgcgctcctt taattcttct ttgtgagttc
     5881 tgcggcccat gcggcgcagg atccggtcac tggcatgctg gatggggata tccagataat
     5941 ggcagacctt ttcctctgat ttaatggcct cgatcagctc atccgtaatc tcctccggat
     6001 agcagtactg gatgcggatc cagtaaatcc ccggaatctt tgccagctca tggagcagct
     6061 ttggaaggga tttttcccca taaatgtctt ttccataaag agtggtctcc tgagccacca
     6121 ggatcagttc ccgcactccg ttgtcagcca gctctctggc ctcctctaac agccgttcca
     6181 tgggaacgct gcggtaaggc cctcttaagg acgggataat gcagtaggta cagcgcttat
     6241 cacagccctc ggcaatcttt aaaaaggcgt agtagccgcc ggtagtcacc acacgctttt
     6301 tagctgtgtg gggcagctcg ctcagactgt gaaaaactga aatgtggctg tcagccgttc
     6361 cttccccgtc cctctcatct tctccaaaaa gctccgtcag aacccgtccg atctcttcgt
     6421 aggaggtggt tcctaaaatt gcgtctacct ccgggatctc ctctgtgatc tcctctttat
     6481 agcgctgagc cagacagcct gccacgatca gggcacggca tttaccctct tcctttaagc
     6541 gggccatctc cagaatggta tttacgcttt cttccttggc gtccccgata aaacagcagg
     6601 tattgatcaa aacagcatcc gcctctgttt catcatctgt aaaggttacg ccaaggcctc
     6661 cggctacgcc taacattttt tctgtatcca ccaggttttt atcacagcct aaggacacac
     6721 agaataattt tatcttcttc ataaatgcat tttactcctg attaacattt aagccatctt
     6781 cccgtgtctg ccgggcgtag atcgccccgg cgtttcaggc acaggagacg gcttcttctt
     6841 tctcttattc gttttgggca gcctcttttg catcagcttc ttcttcctct gttgtctcct
     6901 cttccggaag aatctttacc tgctgatggt acagctcgtc aatggcgatc gacagaggct
     6961 ttttccctgc tgtgccggga actaagggtt cttcccctcc aatcagctgt cttgcacgct
     7021 tggaggctgc aatgacgata gaatagcggc tctgtaccac cggctgctcg cccggttcga
     7081 tatcgctgtt tacaacgttg atcagatctg tgtatgatgg atgtaacatt ctacattccc
     7141 ctttcaattt taaatatata atcgctctgt ctgcggcccg aaaatgatcc cggcttttaa
     7201 cagggcagtt tgctgacatt agtttctctc atccagatga tacaggtcct cccgcatctt
     7261 tccaaggaaa tccagatgat aggaggtttt ttcgtgctgc agctggatca gctggtgcat
     7321 tttttccaca catgtatcaa tctcatcatt gatcaggagg taatcatagg cttccatgcc
     7381 gtccgcctcc tcagccgctc tctttaagcg ggcgttgatc acctcaatgc tttccgtacc
     7441 gcggcccacc aggcggcgct ttaactcctc tgcgctgggc ggcgtaacaa aaagaagcag
     7501 cgcctcagga tagcgcttct ttacctttaa agcgccctgg atctcaatct ccaggatcac
     7561 gtccttgccc tgagccatct tttcttctac atactccttg ggagtgccgt aatagtgatt
     7621 tacatacttg gcatactcga tcagcttatg ctcatcccgc agcttcttaa actcttcttc
     7681 cgaaaggaaa aagtattctc ttccgttttc ttcaccttcc cgtggttttc tcgtagtggc
     7741 cgagatcgaa agcgcataat tatcataacg ccttaaaagc tccttcatca aggttccctt
     7801 gcccgctccc gaaaatccgg atacaaccac taaaattccc tgctggttca tgggatctct
     7861 cctcactttc atctctattc gatgttctgg atctgttctc ttaccttttc aatctccgtt
     7921 ttcagggcaa tggccttatc ggaaagctcc agatcgtttg actttgataa aatggtattg
     7981 gcctcccggt tcatctcctg agcgataaaa tcaagctttc tgcctacact ttcaccgctt
     8041 aaaagctccc gtttcgtatt ttcaatgtgg cttctcaaac gtaccgtttc ctcatctaca
     8101 cagatcttgt ctgcatagat caccacctca gaggcaatcc gtccctcgtc taccgcagtt
     8161 ccgtttaaca gctcctttac cttttcctcc aggcgtgtcc ggtactcgga aataatctgc
     8221 ggagaacggg ccttgataaa atcaaccagc tctgtcatgt aatccagctt gcctaaaagg
     8281 tcatgtttca ggtgttcgcc ctcaatctct ctggaagcca caaacttttc ggcggcctcc
     8341 tccatagcgg atgaaaggat ctcccagagg tgctcctcgt catccggggt ctgctccatg
     8401 gtcagaacct ccgggcatct ggcaagcgtg gatacatgga tatcgtttgg aatgtgaaat
     8461 tcctcctcca tccgtttaaa atgctccata tactctgctg ccagagcacg gttgtacttt
     8521 aagcagaggt ttccctccgt atagtcctca taattgataa aaatatccac ctttccccgc
     8581 tggatgtact tcttaagcag ggtacggatg gcagcctcga agtaattgaa cttcttcggc
     8641 atcttaatgc tcagatccag atatctgtgg ttcactgcct tcatctccac agaaatctta
     8701 tactcatcag tagcattctc gcatcttcca aagccggtca tgctttttaa cataaaggaa
     8761 tgcctctctt tcatcgttac atagttatac agtattataa ttcatctgtg ggggcaagtc
     8821 aacttttttt caaaaagagg agacgcgcct tctgagcgtg tctcccctga aaaactttat
     8881 aaaatggtac tcatttaatt caccttggag ccgcaggccg tgcagaaagc atccccctct
     8941 gccagcgaag cgccgcagga agggcagaac cgtccgcctg cgctggatat ttccagaggc
     9001 tccagcttag agccgcattt attgcagaat acgctgtcca gcgtaataat ctgccggcag
     9061 tgctcgcaca tgcgcagtcc ctgagccgcc agcttgttgc gctccagcgt ctccttctgc
     9121 tcctccagag cttttatctg gtttataaac tccccgtatt ccggctcaca gtcctccctg
     9181 tgcttttcca gatacacggt tccgatctga atcagcgctt ccctgatctg cccttccacc
     9241 gcttcaatct gctggtcaaa ctttccggcc gactggctgc tttcccctct gaccaaattg
     9301 gtattttctt taatcgtatc aaacagtccc atatgcccaa tcctcctact gtattccaaa
     9361 ccaggcgtct aaaagcccgc tgtcaacgct gctgatctcc cagcgcttct cctctttatt
     9421 atatgtaaat tctacggttc ccgtataggt tttgtctgcc gtactgcttt ctgctttttc
     9481 ttcaaatccg ttcagtataa gagaagcgtc cccaaacgcc atatccgtca ggttcatcca
     9541 cagattcttc ctgtttatta ccagattcac aagctcggac aggcctgtgt agtcctctat
     9601 tacccgacgg tttatatcgg ggatatccag ataatgcact gttacggctg cgctgacgat
     9661 cccgtccttc tttttcgctt tttccgcctc aaaataagaa cgactggtca cagactctgc
     9721 cagctgttcc atattagcgg ctctggaaaa ggactctgaa agaacctttc ccagttttga
     9781 tgaaatgtca cttacctctt tatcttcaaa attttctccc agctttccca gagaaagctt
     9841 tatattctga aaaaactgtt cggtcagatc atttaccata tagtcaaagc aggaacggta
     9901 cagattttca ttttccgaat attcgctatg ctccgcaaaa tattcctcca tcatagcctg
     9961 cgtatcaatc tttcccgcac tgggctttaa taaaagaacg gcaaaaacaa gcacaagaac
    10021 cgctcccaga agccctgcag cctttatgga aatctgcggg cgcttccatg tgaaggatac
    10081 gccttttttc tctgcctttt tcgtacttcc tgtgtttaca cgcatctttt caccgcattt
    10141 ggggcaaaac tgcgcgccat tcttaacgtc atttccacac ttttccctcc tgataaaagg
    10201 tctttgcaaa taaatgtctc agattgtgag ggaatacctt tttcggatca acgcctgcct
    10261 tttcgcagag ctttttcatt gccgcccata tattgcttcg gtttaaggct cgttttcctc
    10321 tggtgataaa gacagggccg tccgcaatcc ggtgtttttt catatactcc ttcaaaatcc
    10381 gccgcagctt ctccggaaga aagacaatgc gctttttatt tttgcagctt acctctgccc
    10441 gccctgcttt caccgcctct gccgtgataa atgcaagctc tgacacccgt atccccgtag
    10501 cgcagatggt ctgcatcaca agcttcaggc gctccttccc ctgtctttgc gcttcctcaa
    10561 tcaggcgcat atattcttcc cgtgataaat cctcctccgg ttccctgtag atccgcttct
    10621 gctgccggaa gggttttacc ctcagctcct cccagccgcg aaaatcaaaa tacccgttta
    10681 aagccgcaag catggaattg gcagaggata ccgcatagca tgtccccaga tattccttcc
    10741 attccaacac gcggagccgg tcgatctcct tttctccatt ccacctgcaa aatgctctta
    10801 catctcttat atatttttca atggtagccc ggcttctttc attttcaagc atatcccggc
    10861 ggtaaacctc cagttctttt tcgcaaataa catatttctt cccttcaccc attccagctc
    10921 tcctgccttt cttttttaca accattatat tgctcttttc cttgagtttc cgcattttac
    10981 agtgtgatct gggggtctgg tgtgcagggc gactttgaag gtcattagaa aaccttagag
    11041 gattaaattt tttgttaaaa gccatatcaa acctgtttga gcgcagcgag tttttgatat
    11101 ggcttttgtt tttagaaaaa tt
//
LOCUS       DBMDUH010000085        11119 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_2939_length_11119_cov_5.135052, whole genome shotgun sequence.
ACCESSION   DBMDUH010000085 DBMDUH010000000
VERSION     DBMDUH010000085.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 11119)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11119)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11119
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2939_length_11119_cov_5.135052"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="ACZPCG_08285"
     CDS             <1..546
                     /locus_tag="ACZPCG_08285"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1285096.1"
                     /translation="ETGIPFRTPDESLPMQSRYQFREITKSGVPQMFSMILKRMEWMD
                     EKGIQQWNAAKYDTIYPLSYYEEKRLNGEVFVLVSKDTKELISAAVLKEQDDRWNDSE
                     PALYLHNFVTRVEAPGAGSTFLRFAEEYALKKGKKYLRLDSAADNPSLARYYTSQGFV
                     PAGTCTEGGYNGILRQKELLP"
     gene            618..983
                     /locus_tag="ACZPCG_08290"
     CDS             618..983
                     /locus_tag="ACZPCG_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HIR1285097.1"
                     /translation="MEFRNLLNIKNRDELRQWLLENHNKEPECWVVAKRGRPVDDETF
                     WYIDAVEEAMCFGWIDSTTKKMDNGVAAQRLAPRRKGSLWSELNKERCRRMERFGRMT
                     NAGRAVLPDMSEKGFVPRC"
     gene            complement(1382..2884)
                     /locus_tag="ACZPCG_08295"
     CDS             complement(1382..2884)
                     /locus_tag="ACZPCG_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012200074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR1285098.1"
                     /translation="MNKKISDFLFKNKVVILFLLLCVGATAASKQPLTFVIPELFTRI
                     ARNAFIVLALIIPVITGLGLNFGIVVGAMAAQIALFLTTYWGFTGVGGFFITVALATP
                     IALVFGFLVGKLFNLVKGNEMIAGLILGYFADGIYQLIFLFVFGGVIAMNNPTLMIAT
                     GVGVKNTIDLKDTVKYALDTIPMLTVLEIGFYLVIAGLLFTTIHKRIKKQAVNWKDTA
                     LKAAAAVFVYALTFIGPVEQFLSSDRLLLLSAVELGCLSVVLWQLFQIVRHKMTKHTG
                     AGSFGKRDTFDIRRALVFIVLAGAVYGLTYIPALFNVLIAVQLPVMTYICIGALCVFN
                     NLLMRTRLGQNMRAVGQNRAVANSAGIDVDKTRIIAMLFSTVLASWGQLIYLQNIGTM
                     STYGAHTQVGQFAIAALLVGGASVQKATNKNALLGIVLFHTLFIVSPLAGKELFGDPV
                     IGEYFRVFVSYGVIAMALAMHAWKKPGTDKSKDGPSEFESQMKPASLGKV"
     gene            complement(2877..3968)
                     /locus_tag="ACZPCG_08300"
     CDS             complement(2877..3968)
                     /locus_tag="ACZPCG_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012200075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR1285099.1"
                     /translation="MTDSVKKMIRKFGLPRIIIVLFFLFLVVCAGAFQMNLPQFFSDV
                     LRRWGMYGVLALAMVPAIQCGIGPNFGITMGIVGGLLGGLVVIQLDIAQNPALVAIHP
                     LFAAWVAILAAIVIGVILAVGIGYLYGQLLNRVKGSEMTVTTYVGFSVIAFMNMVWML
                     LPFDNGELIWPNAGKGLRNTISLASSYSGVMNDTLAFSIGDFFVPTGLLLFFFLMCFL
                     VWLFMRSKTGIAMSAAGTNPMFAKAAGINVNRMRLVGTILSTVLAAVGILVYAQAFGF
                     IQLYNGPMMMGFTSVAAVLIGGATPRRASIAHVLIGTFLFQGIMALGLPVVNQFIPES
                     NLSEVMRLIISNGIILYALAQAKGGDSRE"
     gene            complement(3961..5541)
                     /locus_tag="ACZPCG_08305"
     CDS             complement(3961..5541)
                     /locus_tag="ACZPCG_08305"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR1285100.1"
                     /translation="MDHIVKEYFGNRVLKGVDLAVRPGEIHALMGENGAGKSTLMNIL
                     FGMPVIHNTGGFEGTIEIDGKEVQINSPVKAMELGIGMVHQEFMLIPGFSVTENIKVG
                     REISHANLASRVFGESMETLDFDSMDRDARKALKTIGLEIEEYVKVAGLPVGYMQFIE
                     IARELDKTGLKILVFDEPTAVLTESEAKRLLEAMKVIAASGIAIIFITHRLDEVMAVA
                     DSMTVLRDGEFVARREIRDTNVVEIAELMIGRKVEKLLDDDIPDERVIRDDEIAVSLR
                     NYRVDMPGEQVNGIDMDIRKGEIFGIGGLAGQGKLGIPNGIMGLYKASGEVRINGEAL
                     NLDKLGDALDHKVAFVSEDRRGVGLLLNESIEQNIVFSAMQTNGRFLKKIGWFHLYDA
                     AAAKVHATHMVETLDIRCTGIGQAAGRLSGGNQQKVCLARALTLEPDILFVSEPTRGI
                     DIGAKKLVLEYLAKLNRETGMTVIIVSSELVELRSISDRIAIISDGKLSGILKPDDSD
                     ADFGLAMSGTRKGGMEND"
     gene            complement(5717..6955)
                     /locus_tag="ACZPCG_08310"
     CDS             complement(5717..6955)
                     /locus_tag="ACZPCG_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012200077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HIR1285101.1"
                     /translation="MKKRFLSLTLAAAMVMSLAGCRSAEPAATTAAPASEATTAAPAD
                     GEKKEGASEAETASAGDFKIGIITGTASQGDEEITQANKMKEKYGDMVVTSTYPDNFT
                     TETETLISNAVAMASDPAVKAIVWCQAVPGTAAAIDKVRETRPDMIFIAGTPGEDPGV
                     INPKADIVLQVDEPGCGVVIPPQAKAQGAKTLIHYSFPRHMSYALIVARHENMKKYCA
                     ENGIELVDVTAPDPTGDSGITGAQQFILEDVPRQIEKYGKDTVFFTTNCGMQEPLIRS
                     VFENGAIYSLQCCPSPFHAFPAALNIDMSGHEADVTYMMEQLQAKVDEYGMNGRVSTW
                     GVPCNMLFVNAGVEYAKKVLEGETNGEVLDEEVLKATIQECAKEYTPDANMTIEHYVD
                     DSGNEKDNHFLVMSDFVTFK"
     gene            complement(7383..8987)
                     /gene="pckA"
                     /locus_tag="ACZPCG_08315"
     CDS             complement(7383..8987)
                     /gene="pckA"
                     /locus_tag="ACZPCG_08315"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009247260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HIR1285102.1"
                     /translation="MAEINLSKYGITGTTEIVHNPSYELLFEEETRPELEGYEKGQVS
                     ELGAVNVMTGIYTGRSPKDKFIVVDENSKDTVWWTSDEYKNDNHPASEEAWKACKEIA
                     LKELSNKKLYVVDAFCGANKDTRMAIRFIVEVAWQAHFVTNMFIQPTAEELENFEPDF
                     VVYNASKAKVENYKELGLNSETAAMFNITSREQVIINTWYGGEMKKGMFSMMNYYLPL
                     KGIASMHCSANTDMNGENTAIFFGLSGTGKTTLSTDPKRLLIGDDEHGWDDNGVFNFE
                     GGCYAKVINLDKESEPDIYNAIRRDALLENVTLDAEGKIDFADDSVTENTRVSYPINH
                     IENIVRPVSSAPAAKNVIFLSADAFGVLPPVSVLTPEQTQYYFLSGFTAKLAGTERGI
                     TEPTPTFSACFGQAFLELHPTKYAEELVKRMEKSGAKAYLVNTGWNGTGKRISIKDTR
                     GIIDAILNGDIKNAPTKTIPYFNFEVPTELPGVDPKILDPRDTYADAAEWDAKAQDLA
                     GRFIKNFAKYEGNEAGKALVAAGPQK"
     gene            complement(9221..9685)
                     /locus_tag="ACZPCG_08320"
     CDS             complement(9221..9685)
                     /locus_tag="ACZPCG_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NanQ anomerase/TabA/YiaL family protein"
                     /protein_id="HIR1285103.1"
                     /translation="MIFDEKKNLNFYRNLGIEGRYAKAVDFLVNTDLAALEAGKHEID
                     GTNVYANVTSYTTIPWEEAKYESHEHYTDIQYVIEGSEVMTYAPTHEMEVKTPYNPDK
                     DVTFYENTVPGLQAVVKAGQYMIFHPWDAHKPKAADGEPAPIKKVIVKIKED"
     gene            complement(9792..10634)
                     /locus_tag="ACZPCG_08325"
     CDS             complement(9792..10634)
                     /locus_tag="ACZPCG_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HIR1285104.1"
                     /translation="MLTLHSFLQSVVLFVKALAVKVKKEQYRTSSVVMAGAVVIAIVT
                     FGANGFGGSGRNAMAAPRIQESAEEPEEPEEEVVSGGSSFVTEAKIQFGLLNTDSEGQ
                     HLAGALLEQDVREKQRREAAAQTEIEALQKQILKEKEEEEARRKAEEERRAARRIRIS
                     DEDYQILLRIVQAEAGICDDKGKILVADVIINRVLSKRFPNTVKGVVYQRNQFQPVSN
                     GRINTVKVTPDTIRCVDRALDGEDYSNGAIYFMNRRASGKQASWFDSSLTYLFAHDGH
                     EFFR"
BASE COUNT         2944 a         2772 c         2341 g         3062 t
ORIGIN      
        1 gaaactggta ttcccttccg aacgccagat gaaagtctcc ccatgcaaag cagatatcaa
       61 ttcagagaaa tcacaaaatc aggggttccc cagatgtttt caatgatcct taagcggatg
      121 gagtggatgg acgaaaaagg gatacagcag tggaatgctg caaagtatga tacgatctat
      181 cctctgtctt attatgaaga aaaacgctta aacggcgagg tgtttgtcct tgtatcaaag
      241 gacactaaag aactcatcag cgctgctgta ttaaaagagc aggatgaccg ctggaatgac
      301 agcgaacccg ccctttatct ccacaatttt gtaacccgtg tggaagctcc cggcgccgga
      361 agtacctttc tccggttcgc agaggaatac gccttaaaga agggaaagaa atatctccgt
      421 ctggactcgg cagcggacaa cccttctctt gcccgttact acacctccca gggctttgtg
      481 cccgcaggaa cgtgtacaga aggcggctat aacggcattt taaggcaaaa agaactgctt
      541 ccctgaagaa tccctgctgg gaaatctatg gcatatggag gtgcggcaga agcagaaggg
      601 ctatctgaat aacgcgaatg gaatttagaa atttattgaa tataaaaaat cgtgatgagt
      661 tacggcaatg gcttttggaa aaccataata aagagcctga atgttgggta gttgcaaagc
      721 gtgggcgtcc tgttgatgat gaaacatttt ggtatataga cgcagttgaa gaagcaatgt
      781 gctttggttg gattgacagc acaacaaaga aaatggataa cggagttgca gcacaaagat
      841 tagcgcctcg aagaaaggga agtttatggt ctgaattgaa caaagaacgc tgccgcagaa
      901 tggaacgatt cggacgaatg acaaatgccg gtcgtgctgt gttaccagat atgagtgaga
      961 agggatttgt gcccagatgc taatgttaga tgtaattttg tctataatag aataacaaag
     1021 gaagtagata ttacaaataa caatgtgctt attgaaaaaa tattgccaat tccaatttac
     1081 tggcttgata aaaaacttaa agaaaacgga atgttaaaat ctaaggaatg taaaattagt
     1141 tattagggca agactggttt gttcttaaaa atcccagttt gtatataccc ttaatacaac
     1201 aaaatgccgt atccagacgg agtgaaccag atgcagcgac agctgaaatg tattcactta
     1261 aaccagaaaa gagggtgctg caaaatcatc cacaatcgaa tgattgagca gcaccctctt
     1321 tcctgttgtt caggccacta tgccttactc tttgacgtct attaaaaggt atttttcatt
     1381 tctaaacttt tcctaacgaa gccggcttca tctggctttc aaactcactt ggcccatctt
     1441 ttgacttgtc tgtgccgggc tttttccatg catgcatagc cagtgccatg gcgattacgc
     1501 cataggaaac gaatacgcgg aaatactcac cgatcacagg atctccgaac agttcctttc
     1561 ctgctaacgg agatacgata aacagggtat ggaacagaac gatgcctaaa agagcatttt
     1621 tatttgtcgc cttctgcacg gaagcgccgc ctaccagaag cgctgcaata gcaaactgcc
     1681 ccacctgggt atgcgcacca taggtagaca tggtgccgat attctgcaga taaataagct
     1741 gtccccagct ggcaagcacg gtagaaaaca gcatggcgat aatacgggtc ttatccacat
     1801 caatacctgc agagtttgct accgcacggt tctggcctac tgccctcata ttctgtccca
     1861 gacgggtccg catcagcaga ttgttgaata cgcagagagc tccaatacag atataggtca
     1921 tcacaggaag ctgtacggca atcagcacgt taaacagtgc gggaatatag gtaagtccat
     1981 atactgcacc agccagaacg ataaagacaa gggctctgcg tatatcaaag gtgtctcttt
     2041 tgccaaaact tccggctccc gtatgtttcg tcattttatg tctcacgatc tgaaacagct
     2101 gccacagcac aacgctcaga cagcccagct ctaccgccga caaaagcagc agacggtcag
     2161 aagataagaa ctgctccacc ggtccgataa aagtcagggc atagacaaaa actgctgccg
     2221 ccgccttaag cgctgtatcc ttccagttca ctgcctgttt tttgatccgc ttgtgaatgg
     2281 tggtaaagag aagtcctgca attaccagat agaagccaat ttccagaacc gtcagcatgg
     2341 gaatggtatc cagagcatat tttaccgtat cctttaggtc aatggtattc tttacgccta
     2401 ctcctgtggc aatcatcagc gtaggattat tcatagcaat cacgccgccg aatacgaaca
     2461 ggaagatcag ctggtaaata ccatctgcaa aatatcccag gatcaggccc gcgatcatct
     2521 catttccctt taccaggttg aagagttttc ctaccaggaa gccgaacacc agcgcaatag
     2581 gggtagccag ggcaacggta atgaaaaagc cgccaactcc cgtaaaaccc cagtaagtgg
     2641 taaggaataa ggcaatctgt gccgccatgg cgcctactac aataccaaaa ttcagaccca
     2701 gacccgtaat aaccggaatg atcagtgcca gcacgataaa cgcatttctg gcgattctgg
     2761 taaacagctc cgggatcaca aaggtcagag gctgtttcga tgcagccgta gctcctacac
     2821 aaagaagaag gaacaggatc actaccttat ttttaaataa gaaatcagat atttttttat
     2881 tcacggctgt cacctccctt tgcctgtgcc agtgcataca gaataatacc attagagatg
     2941 atcagacgca ttacctctga taagttgctc tccggaatga actggtttac aaccggaagg
     3001 cccagagcca tgatgccctg aaacaggaac gtaccgatca gcacgtgggc aatggaggcc
     3061 cgccttggag tagcgcctcc gatcagtacg gctgctacag aggtaaagcc catcatcata
     3121 ggaccattgt acagctggat gaagccgaag gcctgtgcat ataccaggat acctactgca
     3181 gccaggacgg tcgaaagaat ggtacccacc agacgcatcc ggtttacatt gatgcctgcg
     3241 gccttggcaa acatgggatt cgtacctgcc gcactcatgg caattccagt cttagaacgc
     3301 ataaagagcc acaccagaaa gcacatgagg aagaaaaaca gaagaagtcc ggtaggaaca
     3361 aaaaagtctc caatggaaaa agcaagggtg tcattcataa caccgctgta ggaggaagca
     3421 agggaaattg tatttctcag tccctttccg gcattgggcc agatcagctc accattgtcg
     3481 aagggaagaa gcatccagac catattcatg aaagcgatca cggaaaatcc tacataggta
     3541 gtaacggtca tttcagagcc ctttacccgg tttaagagct ggccgtagag gtatccgatg
     3601 cccactgcca gaatcacgcc gatcacaatg gcagccagaa tagccaccca ggctgcaaag
     3661 agaggatgga ttgctaccag agcgggattt tgtgcaatat ccagctggat cacaaccaga
     3721 cctcccaaaa gccctcctac aatacccatg gtgataccaa agttcggtcc gatcccgcac
     3781 tggatcgccg gcaccattgc aagagctaaa acaccgtaca ttccccagcg gcggagcaca
     3841 tcactgaaaa actggggcag attcatctgg aatgcccctg cacataccac cagaaacaga
     3901 aaaaagagaa cgataataat acgcggaagg ccgaatttac ggatcatctt cttaacagaa
     3961 tcagtcattt tccattcctc ctttccgtgt tccggacata gccagaccga agtcggcatc
     4021 cgaatcgtcc ggttttaaaa tacccgacag ctttccatcg gaaatgatcg caatccggtc
     4081 tgaaatcgac cgcagttcta ccagctcaga agaaacgatg atcaccgtca tgcccgtctc
     4141 gcggttcagt tttgccagat actcaagcac cagcttcttg gcaccaatgt caattcctct
     4201 ggtaggctcc gatacaaaca ggatatccgg ttccagagtc agtgctcgtg ccagacatac
     4261 tttctgctga ttgccgccgg aaagacggcc tgccgcctgc ccgataccag tacagcggat
     4321 atcaagcgtt tctaccatat gagtggcatg aactttggca gcggcagcat catataaatg
     4381 aaaccaaccg atctttttta aaaatctgcc gttggtctgc atggcagaaa agacgatatt
     4441 ctgctcaatg ctttcgttca agagaaggcc cacaccgcgg cggtcctctg atacaaaggc
     4501 taccttatga tccagggcat ctcccagttt atccagattt aaggcctcgc cattaatcct
     4561 cacttcaccg ctggccttat aaagccccat gatgccgtta gggatgccaa gtttaccctg
     4621 gcctgccagg cctccaatcc caaagatttc tccttttcgg atatccatat caatcccatt
     4681 gacctgctcg cccggcatat ctactctgta atttcttaag ctgacagcaa tttcatcgtc
     4741 ccggatcacc cgttcatccg gtatatcatc atctaacagc ttctccactt tgcgtccgat
     4801 catcagttct gcgatctcaa ccacatttgt atccctgatc tcacgtctgg ctacaaattc
     4861 tccatccctg agaaccgtca tactatccgc cactgccatg acctcatcca atcggtgggt
     4921 gataaaaata atggcaattc cgctggcggc gatcactttc atggcctcca gcaagcgttt
     4981 agcctctgat tcggtcagca ctgcagttgg ttcgtcaaat accagtatct taagtcccgt
     5041 tttatcaagc tctctggcga tctcaataaa ctgcatatat cctacgggaa gtcctgctac
     5101 ttttacatac tcttcaatct caagcccaat ggttttaagg gcctttcttg catcccgatc
     5161 catagaatca aaatccaggg tttccatcga ttctccaaat acacggctgg ccaggtttgc
     5221 atgactgatt tcccggccca ccttaatatt ttccgtaacg gaaaatcccg gaatcagcat
     5281 aaactcctga tgtaccatac cgattcccag ctccatggcc tttaccgggc tgttgatctg
     5341 cacttccttt ccgtcaatct caatggttcc ttcaaaaccg cctgtgttgt gaatgaccgg
     5401 cataccaaac aggatattca tcagggtgga ttttcctgcg ccgttttctc ccatcagcgc
     5461 atgaatctca ccgggacgta ccgcaaggtc aactcccttc aggacacggt tcccgaaata
     5521 ttctttcaca atatgatcca ttttcaggac ataggtttct tttgcatttt ccaaaatacc
     5581 aacctctttc tttttgactt ctttttctac cgccctccat cagggaaaaa tgaaaaaagg
     5641 agtccgccag cttctgcccc aaagacgcca gccggcgaac cccctgcttc ttatatcagc
     5701 cccattgcgg tcaagtctat ttaaatgtaa cgaaatctga cattaccagg aagtggttat
     5761 ccttttcgtt tccgctgtca tctacataat gctcaatggt catatttgca tccggtgtat
     5821 actcttttgc acattcctga atggtagcct tcagaacttc ctcatcaaga acctcaccgt
     5881 tggtctcgcc ttccagtacc ttttttgcat actcaacgcc tgcatttaca aacagcatat
     5941 tgcagggaac gccccatgtg gaaacgcggc cgttcattcc atactcgtct acctttgcct
     6001 gaagctgttc catcatatat gtaacgtctg cttcgtgacc ggacatatca atgtttaaag
     6061 ctgccgggaa tgcgtggaac ggagacgggc agcactgtaa ggagtagatc gcgccgtttt
     6121 caaatacgga acggatcaaa ggctcctgca taccgcagtt tgtggtgaag aatacggtgt
     6181 ccttgccata cttttcaatc tggcggggaa catcctccag aataaactgc tgtgcgcctg
     6241 taataccgga atcgccggta ggatcgggag cggttacgtc aaccagctcg attccgtttt
     6301 ctgcacagta tttcttcatg ttctcatgac gtgcaacaat caaagcatag ctcatatgac
     6361 gcgggaaaga gtagtggatc agcgtctttg ctccctgagc ctttgcctga ggcggaatca
     6421 caacaccgca gcctggctcg tcgacctgaa ggacgatatc ggcctttgga ttgataacgc
     6481 ccggatcctc gccgggagta cctgcgatga agatcatatc cggacgggtc tcacgaacct
     6541 tgtcaatggc tgctgcagta ccgggaactg cctggcacca tacgattgcc ttaacagccg
     6601 gatcagaagc catagccact gcattagaga tcagggtttc tgtctctgtg gtgaagttat
     6661 caggataggt agaggtaaca accatgtcac catatttttc tttcatctta tttgcctgag
     6721 tgatctcctc atcaccctgg gaggccgtac cggtaataat accaatctta aaatcccctg
     6781 ccgatgctgt ttctgcttct gaggccccct ctttcttttc cccatcagcc ggtgcagccg
     6841 tcgttgcctc tgatgccggt gcagctgttg tcgctgccgg ttctgcgctg cggcatcctg
     6901 ctaaagacat taccattgca gctgccagtg tcaaactgag aaaacgtttt ttcattcctt
     6961 ctcctccttt tttaagtcgg gtatcttttt atataccccg tttttaaccc gccctgtttt
     7021 tcctttcgcg aattgttcgt attatatcac atggtttgta tcttgtcaaa agacatttgt
     7081 ccgtttggta caatataagt tgatattttt tcagtctttc gcatattgta tacaaagatg
     7141 ttgatataat tccaataaat ttgcaaaaaa ttaactttat aatcagtttt atttgtattt
     7201 tatcttaata ttatcttcta aaatatctat attctaaata tagacgaact ttatttttaa
     7261 cattgtcttt ttttaagaac aatgtttttg aacctggctg gagatcgaac aaagaaaaga
     7321 ggattaaaaa aagacgcagc aaaatgccgc gccttttcag actttcgtcg atctgttttg
     7381 ttttacttct gagggccagc tgcaaccaga gccttgccag cctcattgcc ttcgtatttt
     7441 gcgaagttct taatgaatct gccagccagg tcctgtgcct ttgcatccca ctctgctgca
     7501 tctgcatagg tatctcttgg atccaggatt ttgggatcta cgccgggaag ctcagtaggt
     7561 acttcaaagt tgaagtatgg gatggtcttg gtaggagcat tcttgatatc accgtttagg
     7621 atagcgtcaa tgatgccgcg ggtatcctta atagagatac gctttccggt accattccat
     7681 ccggtattta caagataagc ctttgcgccg ctcttttcca ttctcttaac cagctcttct
     7741 gcatacttgg taggatgtag ctctaagaat gcctggccaa agcatgcaga gaaggtagga
     7801 gtcggctcgg tgatgccgcg ctctgtacct gcaagttttg ctgtaaagcc ggacaggaag
     7861 taatactgtg tctgctcagg agtaagaaca gatactggag gcagtacgcc gaatgcatct
     7921 gcggacagga aaattacatt cttcgctgcc ggagcagagg aaacgggacg tacaatattc
     7981 tcaatatggt tgatcggata agatacacgg gtattctcgg ttacgctgtc atctgcgaaa
     8041 tcgatcttgc cctctgcatc cagagtaacg ttttcaagca gagcatcacg gcgaatggca
     8101 ttataaatgt ccggctcaga ctctttgtcc aggttgataa ccttagcata gcagccaccc
     8161 tcaaagttaa acacgccgtt gtcatcccag ccatgctcgt catcaccgat caaaagacgc
     8221 ttaggatcgg tagacagagt ggtctttccg gtaccggaca ggccgaagaa gatagcggtg
     8281 ttctcgccgt tcatatctgt atttgcggag cagtgcatgg aagcgatgcc tttcagaggc
     8341 aggtagtagt tcatcatgga gaacataccc ttcttcatct caccgccgta ccaggtgttg
     8401 atgataacct gctcacggct ggtaatgttg aacatagcag ctgtctcaga atttaagccc
     8461 agctccttat agttctctac ctttgccttg gaagcattgt aaacaacgaa atcaggctca
     8521 aagttctcta attcctcagc agttggctga ataaacatat tagtaacaaa gtgtgcctgc
     8581 caagctactt ctacgatgaa gcggattgcc atgcgggtat ccttgtttgc gccgcagaat
     8641 gcatctacaa cgtaaagctt cttattggac agctccttta acgcaatctc cttgcaggcc
     8701 ttccatgctt cctcagatgc aggatgattg tcattcttgt actcatcaga agtccaccat
     8761 acagtatctt tggagttctc atctaccacg atgaatttgt cttttggaga acggccggtg
     8821 tagataccag tcataacgtt aactgcgcct aattcactga cctgtccttt ctcgtaacct
     8881 tccagctcgg gtctggtctc ttcctcaaat aacagctcat aagaaggatt gtgtacgatc
     8941 tcagttgttc cggtaatgcc atacttgctt aaattgatct ctgccatact acattaacct
     9001 cttttctttc ctgtgatatg atgtgaaatg atgtaaatgt gaatgtctgg tttataaaac
     9061 caaattcaag tctattatct cactttattg ttaaaaagtc aacaattcca ttttaacaaa
     9121 attctaattt ttacagaaaa tggtacagat atgacaaata ccggccccct ggacaatgtc
     9181 caaacggccg gtaccttatt ctagtaaatg agacagtaca ctagtcttct ttaattttta
     9241 caattacctt cttaataggg gcaggttcac cgtctgctgc ctttggcttg tgtgcatccc
     9301 atggatggaa gatcatgtac tgacctgcct ttacaactgc ctgaaggccg ggaaccgtgt
     9361 tctcataaaa cgttacatct ttatccggat tgtaaggagt ctttacctcc atctcatgtg
     9421 tcggagcata ggtcataacc tcactcccct cgatcacata ctggatatcg gtgtaatgct
     9481 cgtgagactc atactttgcc tcttcccacg gaatcgtagt gtaggaggtc acattggcat
     9541 atacattggt tccgtcgatc tcatgctttc cagcctctaa tgctgccaga tccgtgttca
     9601 caagaaaatc aacggccttt gcgtatcttc cctcaattcc cagattgcgg taaaaattca
     9661 aatttttctt ttcatcaaaa atcatgattc cagtctcctt tgccttctgt attggacagt
     9721 ttcccgtcac ttcacagtat acgccaaagg atgcggaaac tcaacccttt ttgagtcttt
     9781 tctatgggcg gttatctgaa aaattcatga ccgtcatgtg caaagagata ggtaagactg
     9841 gaatcaaacc aggaagcctg ttttcccgaa gccctgcggt tcataaagta aatagccccg
     9901 tttgagtaat cctctccatc cagggcgcgg tctacgcagc ggatggtatc cggcgtcacc
     9961 tttacggtgt tgatcctgcc atttgatacg ggctggaact ggtttctctg gtatacaacc
    10021 ccttttaccg tattggggaa ccgcttggaa aggacacggt taatgatcac atctgccacc
    10081 agaatcttcc ccttatcatc acagatgcct gcttcggcct gtacaatacg cagcaggatc
    10141 tggtaatcct cgtctgaaat ccggatccgt ctggcggccc gccgctcctc ctcagccttt
    10201 cttctggctt cctcttcttc cttctctttt aaaatctgct tctgcagcgc ctcaatctct
    10261 gtctgggctg ctgcctctct tctctgtttc tcccggacat cctgttccag cagcgcgcct
    10321 gccagatgct ggccttcact gtctgtattt aaaagtccaa attgtatttt tgcttccgta
    10381 acaaagctgc ttcctccaga taccacttcc tcttccggtt cttccggctc ctctgcgctt
    10441 tcctggatcc tgggcgcagc catggcattt ctaccgcttc cgccaaatcc gtttgcaccg
    10501 aaggttacaa tggcaatgac tacagcccct gccatcacaa cagaagaagt gcggtactgt
    10561 tcttttttga ccttaactgc aagcgccttt acaaagagaa cgaccgactg aaggaatgaa
    10621 tgcaatgtaa gcatacatac tcctcttttc tttcctggga tctgtctgat cccatgtgtc
    10681 aacaagttgc ttttcatcag atccaaaata aggtttattc cccacttttg acctgttttg
    10741 ctgggatgta cgggtattat atgggatttg gttctacccg tcaacagagg ttttacaact
    10801 ttgttacatt ttttttgcat caggaaataa tttccgatta tattgtgctt attgtatact
    10861 ttttcttggt atcatcctgt tttttgaacg aaatatagat tttattttta cgtttttatt
    10921 acattattgt taatatttaa ttttgttatt ttattaacaa tacaatttca accctttatg
    10981 ccacctgttt tgcggctttt ttccgttttt ttccttctgt acttcacgat ttaccctttt
    11041 acattttctg tcgaaatatg ggattaaccc ccacttttaa tagttttgta ataaaaaagt
    11101 ttctgtccga taattctat
//
LOCUS       DBMDUH010000086        11065 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_2942_length_11065_cov_7.817311, whole genome shotgun sequence.
ACCESSION   DBMDUH010000086 DBMDUH010000000
VERSION     DBMDUH010000086.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 11065)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11065)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11065
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2942_length_11065_cov_7.817311"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="ACZPCG_08330"
     CDS             <1..900
                     /locus_tag="ACZPCG_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1285105.1"
                     /translation="VSARSPRFPLILSLFNGILCLAALINMYSVVLRVRTNATIQYSS
                     FMDIYEFVTAIEFILLMFIVPAVTAASISGERERQTLDLMLTTQMTAAQIVTGKLMGA
                     LSSLFVLILSSFPAVAMVFVYGGITWWDALSLIFCYVAVAFMAGSLGIFFSSVFRRST
                     VSTAVTYGTLIAFVAGTYFINRFALSMSAANLDQTIAVYAMEAPAIRASSGGAFYLFL
                     MNPAFTFMAVIGNQAGRSTSFASVCSYFGMENTGFVMEHWVLLSVLLQLAAGALLLWE
                     AVQFLEPVKKKGKRKNKRMRHTF"
     gene            1041..1331
                     /locus_tag="ACZPCG_08335"
     CDS             1041..1331
                     /locus_tag="ACZPCG_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HIR1285106.1"
                     /translation="MNENYTYILRCADGTYYCGWTNNLDRRLKAHNEGKGAKYTRSRR
                     PVALVYYEAFSTKEEAMRREYEIKQLPRKKKEELIFKQQTDTSSCNGRDFRP"
     gene            complement(1279..1950)
                     /gene="trhA"
                     /locus_tag="ACZPCG_08340"
     CDS             complement(1279..1950)
                     /gene="trhA"
                     /locus_tag="ACZPCG_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008724134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAQR family membrane homeostasis protein TrhA"
                     /protein_id="HIR1285107.1"
                     /translation="MTQKIKHNLRKIKDPGSAMTHFIAMVLAAAAAVPLLIKGSRSPE
                     QFHLAALAVFILSMIGLYGASTIYHTLDISPKINQLLRKIDHMMIFVLIAGTYTPVCL
                     IVLGDQTGWALLALVWIIAVLGIFINAFWITCPKWFSSMIYIAMGWVCILAFSKILAA
                     LPKAGFLWLLAGGIIYTLGGVIYALKLPIFNARHQYFGSHEIFHLFVMGGSLCHYMMM
                     YQFVA"
     gene            2119..2874
                     /locus_tag="ACZPCG_08345"
     CDS             2119..2874
                     /locus_tag="ACZPCG_08345"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015573435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR1285108.1"
                     /translation="MLSVLYEDSDILVAVKPAGVESQSARGFAKDMVSEIRRYLTIQA
                     RNPQNIHKLTTLSSKGQGKNQPPYVGVIHRLDRPVSGIMVYARNQKAAASLSSLMQQG
                     KMEKWYKAVVCGKPVDNHGVYVDYLRQNQGENRSEIVDKSAAGSKRAELSYTAEASTE
                     RCDAGESREYSLVRIRLLTGRHHQIRVQFSGHGTPLYGDDKYGEKTAPGQPDLLALCA
                     FRLSFPHPVTGKRMEFEMEPSGGAFEWFKENPS"
     gene            2967..3695
                     /locus_tag="ACZPCG_08350"
     CDS             2967..3695
                     /locus_tag="ACZPCG_08350"
                     /inference="COORDINATES: protein motif:HMM:NF018604.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HIR1285109.1"
                     /translation="MERISESGLGRLLHYNMTCIGGFMGTFAVCGFGGNFASAHTGNI
                     MSLTKDLVGCDLIQAGIRLGALVLFSLSIAAAYLINRMVKPDMERLCLIVDVGGFLLC
                     LLLPKGLPDLVRLYPIFIASSFQWGTFSGTDGYNSSTIFTTNNLKQCVLSWTQYLLDH
                     EETMRRRAWFYTITVGAFTVGALIGCMAVLRMGGRGVVWGLGLILAAGVLSFLKKRQI
                     EKGESSKDRTASPAKDKKVHAPAV"
     gene            complement(3827..5383)
                     /locus_tag="ACZPCG_08355"
     CDS             complement(3827..5383)
                     /locus_tag="ACZPCG_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIR1285110.1"
                     /translation="MSILNVEHLSHGFGDRAIFQDVSFRLLKGEHIGLVGANGEGKST
                     FMNIITGKLMPDEGKVEWAKNVNVGYLDQHTVLEKGMTIREVLKSAFGPLFEMEANMN
                     AICNRMGEASEEEMNEMMEELGTIQDLLMAHDFYIIDSKVDEVGRALGLDEIGMERDV
                     TELSGGQRTKVLLGKLLLQKPDILLLDEPTNYLDVQHIEWLKRYLLDYENAFILISHD
                     IPFLNSVINLVYHMENQRLDRYVGDYDKFQEVYAVKKAQLEAAYKRQQQEIAELEDFV
                     ARNKARVATRNMAMSRQKKLDKMEVIELAKEKPKPEFHFLEARTAGKYIFETKDLVIG
                     YDEPLSRPLNLVMERGQKAVLVGANGIGKTTLLKSILGLTPALSGSVELGDYLSIGYF
                     EQEMAPGNTNTCLDEIWKEFPSYTQYQVRSALAKCGLTTKHIESQVRVLSGGEQAKVR
                     LCKLINRESNVLLLDEPTNHLDVDAKAELKRALKEYKGSILLICHEPEFYEGLATDIW
                     DCRDWALRLS"
     gene            5533..6483
                     /locus_tag="ACZPCG_08360"
     CDS             5533..6483
                     /locus_tag="ACZPCG_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HIR1285111.1"
                     /translation="MDLLYRKTPEGICLERCYTLDGAPVLPERMDGMPVTELDRYFFS
                     QTVRGREVPPEELNGEPELCGSGLDELTLPEYLRKIGPYAFYNCFHLKTLSFWSTVED
                     WGAGVFTGCTGIKHLKIRVVPGRKSCFKEVLSELRQELMADYLNPEGRLLARLVFPEF
                     FEESVENTPARIIMREMHGCGHMYRYCFEDTQFQFREYDHLFPNTVIQERPGLAAQMA
                     VYRLYWPWGLTDEFKDQYWEFLKQHPRETASGLTDRRETAILKWLSWQTEADQAFLEG
                     LLNGIEDQRDPQAASVLLDAVHSRFGGQVKEKKTTRTFKL"
     gene            6510..7904
                     /locus_tag="ACZPCG_08365"
     CDS             6510..7904
                     /locus_tag="ACZPCG_08365"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HIR1285112.1"
                     /translation="MSKKNEAQKLQEELTWSAPHIAKEAPEQKEKAFKYCEGYKEFLN
                     KGKTERECVKEAVKMLKKAGYKPFDRKASYVPGDKVYVVNREKAVIATTFGKKPLKDG
                     LRINGAHIDAPRLDLKPNPLYEKEDIAYFKTHYYGGIRKYQWGTMPLAIHGVVAKKNG
                     ELIEVCIGEKEDEPAFCITDLLPHLAGKQNERPLREGLKGEELNVIIGSIPYEGEEIK
                     EAVKLMVLSLLHEKYGIKEKDFFRAEFELVPAAKARDIGLDRSLVGAYGQDDRVCAYT
                     ALTAEIETKKPAHTTVTILTDKEEIGSEGNTGLNSDYVLHYIEDLAELEHTPVRDILR
                     ASLCLSSDVNAAYDPTFADVFESRNASYINKGCVLTKYTGARGKSASNDASAETMAKV
                     IAIMEEAGVFWQAGELGAVDVGGGGTIAKYVAHMDVDTVDLGVPILSMHSPFELASKL
                     DVYNTYKAFKAFYA"
     gene            7998..9089
                     /gene="tig"
                     /locus_tag="ACZPCG_08370"
     CDS             7998..9089
                     /gene="tig"
                     /locus_tag="ACZPCG_08370"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002593950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HIR1285113.1"
                     /translation="MRKIALFCLCGLTAASILTGCSGSADGNVNLGTVTLSEYKGVTV
                     NIEAPEVTDEEVDTKVETILAQNPNEVEVDRAAKEGDIVNIDFKGTKDGEAFEGGSAE
                     DQDLELGSGKMIEGFESGLIGAKKGETKTLNLTFPEDYREESLAGQPVVFEVTVNSVK
                     EEQEAELNDDFVKRVSKEEYQTVDAYKESIREDLLDQKQSAAEQDMEEGALQSVISSS
                     EFKLSRNGLSVRYNQMVKEYTNQAKMYGMSFSQMAQANGMNEPAMKEYIYDSVEEAAK
                     RELTVEAIAANEGLDQITDEDRELFAQENGMSKDTLINMYGQEQADEYVLQDKVLYYL
                     ASNAVNEAENPAKVSERVLEKTEASPEAE"
     gene            9154..9738
                     /locus_tag="ACZPCG_08375"
     CDS             9154..9738
                     /locus_tag="ACZPCG_08375"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008720099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HIR1285114.1"
                     /translation="MQILKDRIRKDGKIKGGNVLKVDSFLNHQMDIKLFGEIGKEFKR
                     RFADAEVNKILTIEASGIGIACIVAQYFDVPVVFAKKNQTKNIAGEVHTTKVESFTHG
                     RVYDIIVAKEFLGKGDKVLIIDDFLANGKALEGLIALVRDSGAELVGAGIVIEKGFQV
                     GGDLIRSEGIRLESLAIVDSMDEETNTIVFRDDE"
     gene            9716..10522
                     /locus_tag="ACZPCG_08380"
     CDS             9716..10522
                     /locus_tag="ACZPCG_08380"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1285115.1"
                     /translation="MYSGMMNKMKALFFDIDGTLLSEKTRQIPESAKEALKEARRRGH
                     LVFINTGRVYCHLSQIRSEVEADGFLCGCGTCVVVQDQILYRHAIPYERGLQMKRDID
                     ACGLDGILEGTEGCYMHKTPSRIPRVEELKNAIRAGGCVIPHDWEDEGYVFDKIYLGS
                     DENSRPKELFGRMKDMEIIDRGNGFYECVPRGHSKATAIDQVLKHCGISLEDAYVFGD
                     SSNDLSMFRYASNCVLMGHHSPVLEPYASFVTKTVEEDGIAYAMKELGII"
     gene            10557..>11065
                     /locus_tag="ACZPCG_08385"
     CDS             10557..>11065
                     /locus_tag="ACZPCG_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002593953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR1285116.1"
                     /translation="MKQKTWIISALMCAMVIMVCLGVLHLLKEMTENNAPSGTVAEES
                     TDHTEPEIDFAEEEETKEDIAETGPEEEESDVPVEREEGYYGWKQELPQIFPSVPEEV
                     PYEPPRLILATDLHYQSAQADDGGAAFQLFVERGDGKVVEYLPELLEAFMDQVIEEHP
                     SALVLSGDIT"
BASE COUNT         2978 a         2398 c         2951 g         2738 t
ORIGIN      
        1 gtcagcgcca gaagccctag atttcctttg atcctttccc tttttaacgg gattctgtgt
       61 ctggcggctc tgatcaatat gtactcggtg gttctgcggg tccgtaccaa tgccacgatt
      121 cagtacagca gttttatgga tatctacgaa tttgtcacag ccattgagtt tatccttctg
      181 atgttcattg tgcctgccgt cacggcagcc agcatcagcg gcgaacggga gaggcagaca
      241 ttagatctga tgcttaccac ccagatgaca gcggctcaga ttgtgacagg taagctgatg
      301 ggtgctttaa gctccttgtt tgtgctgatc ctgtccagtt ttcctgcggt agccatggtg
      361 tttgtctatg gcggcattac ctggtgggat gctctgtcac tgatcttctg ctatgtggca
      421 gtggctttta tggcgggaag tcttgggatc ttcttttcat ccgtattccg aagatccacg
      481 gtttctacgg ctgtaacata cggcactctg atcgcttttg tggcaggaac gtattttatc
      541 aaccgttttg ccctctcgat gtctgctgcg aatctagacc agaccatagc ggtatatgcg
      601 atggaggcgc ctgccatccg tgcttcttcc ggaggcgctt tttatctctt tttgatgaat
      661 ccggccttta cctttatggc agtcattgga aatcaggccg ggcgcagcac ctcctttgcc
      721 agcgtatgca gctattttgg aatggagaat acaggctttg tgatggagca ctgggttctt
      781 ttaagcgttc ttctacagct tgccgcagga gcgcttcttc tctgggaggc agtgcagttt
      841 ctggagcctg tgaagaagaa aggaaaaaga aaaaataaga ggatgaggca cacattttaa
      901 aaacaggcgt atactgatcg tataaagaat gtctggcgca tccagctggt gctttatcat
      961 agatacagac ctttttaggt ccgggcggtg acggcgcgtt ttgcgcaatc gccgtttaca
     1021 tagaaaacgg atgaaccgat atgaatgaga attataccta tatcctgcgg tgtgcggacg
     1081 gaacgtatta ctgtggctgg accaataacc tggacaggcg tctaaaggcc cacaatgaag
     1141 ggaagggtgc caaatacaca cgttcccgaa gaccggtggc ccttgtctat tatgaggcct
     1201 tttccacaaa agaggaggct atgagacggg aatatgaaat aaaacagctc ccccgaaaga
     1261 aaaaggagga gctgattttt aagcaacaaa ctgatacatc atcatgtaat ggcagagact
     1321 tccgcccatg acaaagagat ggaaaatttc atgggaaccg aaatattggt gtctcgcatt
     1381 gaaaataggc agtttcaggg cgtagatgac gccgcccagg gtgtaaatga tgcctccggc
     1441 cagaagccac agaaagcctg cttttggcag cgctgccagt atctttgaaa aggccaggat
     1501 gcagacccag cccatggcaa tgtagatcat ggaagaaaac catttgggac aggtgatcca
     1561 gaaggcgttg ataaagatcc caaggacagc gatgatccag accagtgcca gaagagccca
     1621 tcccgtctga tctcccagta cgatcaggca tacgggcgtg taggttcctg caatgagcac
     1681 aaagatcatc atgtggtcga tctttcgcag cagctggttg atttttgggg aaatatccag
     1741 cgtgtggtag atggtgctgg ctccatagag gccgatcatg cttaaaataa aaacagccag
     1801 agccgccaaa tgaaactgtt caggagaccg ggaaccctta ataagaagag gaactgctgc
     1861 ggctgctgcc agaaccatgg cgataaaatg ggtcatggcg ctgcccggat cctttatctt
     1921 tcgtaaattg tgttttattt tctgtgtcat gaatgatacc tcctttaaaa atatgacaag
     1981 gctgatataa ctatgatata tagtattcaa aactacatta tgatgtaata tatactatac
     2041 acggaatgga cggaatatgc aatacatttg aaaaaattat ttgattatca aagtaaatgg
     2101 gaagaaaggg gataagatat gctttctgta ttatatgaag attcggatat tctggtagcg
     2161 gtaaagcctg caggagtgga atcgcagtca gccaggggct ttgcaaagga tatggtcagc
     2221 gagatccgca gatatctcac cattcaggcc agaaatccac aaaatatcca caaattaacc
     2281 acattatcct caaaagggca ggggaaaaat cagccgcctt atgtgggagt tatccacaga
     2341 ttggaccggc ctgtgtcggg aattatggtg tatgccagaa atcaaaaggc tgcagcttct
     2401 cttagttccc tgatgcagca gggaaaaatg gaaaaatggt ataaggcagt ggtatgtgga
     2461 aaacctgtgg ataatcatgg tgtatatgtg gattatctca gacagaatca gggagagaac
     2521 cggtctgaaa ttgtggataa atcggcagca ggaagtaaac gggcagagct ttcatatacg
     2581 gcagaggcat cgacagagcg ctgtgacgcg ggggagagca gggaatacag tctggtgagg
     2641 atacgtctgc ttacaggccg ccaccaccag atccgggtac aattttccgg ccatggaaca
     2701 cccctttatg gggatgataa atacggagaa aagacagctc ccggacaacc tgacctattg
     2761 gctctgtgcg ccttccgtct gtccttccct catcctgtaa cgggaaagcg gatggagttt
     2821 gaaatggagc cgtccggagg cgcttttgag tggtttaagg aaaatccatc ttaaaagtga
     2881 agaaaaaatg gtacttttgc agaatgacgc tgcaactatt ttttgttata atggtttgga
     2941 tcgattaaaa ggcaggtaag gggaaaatgg aacggatatc agaatcaggg cttggacggc
     3001 tcctccatta taatatgact tgtataggcg gttttatggg aacctttgca gtctgcgggt
     3061 ttggagggaa ttttgcctcg gcccatacgg gaaacatcat gagtcttaca aaggatctgg
     3121 tggggtgtga tctcatacag gcaggaatcc ggctgggggc tctggtctta ttcagtcttt
     3181 ccattgcggc agcctatctg ataaaccgta tggtaaagcc tgacatggaa cggctctgtc
     3241 tgatcgtgga tgtgggaggg ttccttttgt gcctgcttct tccaaaggga cttcccgatc
     3301 tggtccggct ttaccctatt tttattgcgt caagctttca gtggggaaca ttttccggaa
     3361 cagacggcta taacagctcc actatcttta caaccaataa tttaaagcag tgtgttttga
     3421 gctggactca gtatctctta gaccatgagg aaaccatgcg ccgccgtgcc tggttttata
     3481 ccataaccgt aggggccttc accgtggggg cgctcattgg ctgcatggct gtgctgagga
     3541 tgggtggtcg cggcgttgtg tggggactgg ggcttatttt ggcggcagga gttttgagct
     3601 tcctgaaaaa gcggcagatt gaaaaaggcg agagttccaa agacaggact gcttctccgg
     3661 caaaagataa aaaggttcat gctccagcgg tataaaaact gtgggttctc aaaaacaaga
     3721 aaacaggcat atcagccctg acaaggtgca tttacaccag gttggggctt atatgcctgt
     3781 tttttaacgt gatgttatgc cggacgatgt cccgccggct ttataattat gacagccgaa
     3841 gcgcccagtc tctgcagtcc cagatatccg tagccagtcc ctcatagaac tccggttcat
     3901 ggcagattaa aaggatgctt cctttgtatt cctttaatgc acgtttcagc tccgcctttg
     3961 catccacatc cagatggttg gtaggctcgt ctaaaagcag cacattggat tcacggttga
     4021 tcagtttgca cagacgcacc ttggcctgct cgcctccgct taaaacacgc acctggcttt
     4081 cgatatgctt ggttgtgagg ccgcattttg ccagggcaga ccgaacctga tactgggtat
     4141 aggaaggaaa ttccttccag atctcatcca gacaggtgtt ggtattgccg ggagccattt
     4201 cctgttcaaa atatccgatg gacaggtaat ctcccagctc tacggaaccg gacaaggcag
     4261 gagtcagtcc caggatgctc tttaaaaggg ttgtctttcc gatgccgttg gcacctacca
     4321 gcacagcctt ctgtccccgc tccatcacca ggtttaaagg ccgggaaaga ggctcatcat
     4381 agccgatcac aagatccttt gtttcaaaaa tgtattttcc tgcggtacgg gcttccagga
     4441 aatggaattc aggctttggc ttctcctttg ccagctcaat gacttccatt ttatccagct
     4501 tcttctgccg ggacatggcc atattccggg ttgccacacg ggccttgttg cgggccacaa
     4561 agtcttccag ctctgcgatt tcctgctgct ggcgcttgta ggcagcctcc agctgtgcct
     4621 tctttaccgc atagacctcc tggaatttgt cgtaatcgcc cacataacgg tccagtctct
     4681 ggttttccat atggtacacc agattgataa cgctgtttaa aaacgggata tcatgggaaa
     4741 tgaggataaa tgcattttca taatccagaa gataccgctt cagccattcg atatgctgta
     4801 catccagata gttggtaggc tcgtccaaaa gcaggatatc gggcttttgc agcagcagct
     4861 ttcccaacag caccttggtt ctctggcctc cggaaagctc tgttacatcc cgctccatgc
     4921 cgatctcatc taagcccagg gcgcggccta cctcgtctac cttggaatca ataatataga
     4981 aatcatgggc catcagcaga tcctgtatgg ttcccagttc ttccatcatc tcattcattt
     5041 cctcttcact tgcctcgccc atgcggttac agatggcatt catgtttgct tccatttcaa
     5101 agaggggccc aaaggcgctt tttaagactt cccggatcgt cattcccttt tccaataccg
     5161 tgtgctggtc cagatagccc acatttacat ttttcgccca ttctaccttg ccttcgtcag
     5221 gcatcagctt tccggtgata atgttcataa aggtggactt tccctcgccg tttgctccta
     5281 ccaggccgat atgctcgcct tttaagagac ggaaagatac gtcctgaaaa atagctctgt
     5341 ctccaaagcc atggcttaaa tgctctacgt ttaaaatact catatttcct ttaaactcct
     5401 tatatctgct tgctgagtgt cctgtatatt ccagtcgatt gtacatgaga accggggctg
     5461 tggcaagagg tttgaaggct ttctgtcaaa aatcatcttg aaaaaattct tcaaactgat
     5521 taaactggta ctatggactt attatataga aaaacaccgg aaggaatctg tttggagcgc
     5581 tgttataccc tggacggcgc tcccgttctt ccggagcgta tggatggaat gccggtgaca
     5641 gaactggatc ggtatttttt ttcgcagact gtaagaggac gggaggttcc tccggaagag
     5701 cttaatggag agcctgagct ttgcggatca gggcttgatg agctgactct tccggagtat
     5761 cttagaaaaa taggccctta tgccttttac aactgctttc atttgaaaac cctgtccttc
     5821 tggagtacgg tggaggattg gggagccggt gtatttaccg gttgcacagg gataaagcat
     5881 ctgaagatcc gggtggtacc gggcagaaag tcctgtttta aggaggttct ttccgagctg
     5941 cgccaggaac ttatggctga ttatctgaac ccggagggaa ggcttcttgc cagactggta
     6001 tttcctgagt tttttgagga atcggtggaa aataccccgg ctcggatcat tatgagagaa
     6061 atgcacggct gcggccatat gtaccggtat tgctttgagg atacgcagtt tcagttccgg
     6121 gagtatgacc atctgtttcc taataccgtg atccaggaac ggcccggtct ggcagctcag
     6181 atggcagtgt accgtctgta ctggccctgg ggtctgaccg atgaatttaa agaccagtac
     6241 tgggaatttt taaagcagca tccaagagaa acggcatcag ggctgacgga caggcgggaa
     6301 acagcgattt tgaaatggct gtcctggcag acagaggcag atcaggcctt tctggagggg
     6361 ctgttaaatg ggattgaaga tcagagagat ccccaggctg cgtctgtttt gctggatgcg
     6421 gtacacagcc ggtttggcgg gcaggtaaaa gaaaaaaaga ccacccgcac ctttaaattg
     6481 tagattaact tacaggagga atatataaga tgtcaaagaa aaatgaagca cagaaattac
     6541 aggaggagct gacctggtcc gcaccccata ttgccaagga agcgccggag cagaaggaaa
     6601 aggcatttaa gtactgcgag ggctataagg aatttctgaa caaaggaaag accgaaagag
     6661 agtgcgtaaa ggaagcggta aagatgctta aaaaagcagg ctataagccc tttgaccgca
     6721 aggcttctta tgtacccggc gataaggtat acgttgtaaa ccgggaaaag gcagtgatcg
     6781 ccacaacctt tggaaagaag cctttaaagg atggactgcg catcaacgga gcccatatcg
     6841 acgcgcccag actggattta aagccgaatc cgctctatga aaaggaagat atcgcttatt
     6901 ttaagaccca ttactacggc ggcatccgca aatatcagtg gggaaccatg cctctggcaa
     6961 tccacggcgt tgtagcaaaa aagaacggag agctgatcga ggtctgcatc ggtgaaaaag
     7021 aggatgagcc tgcattctgc attacagatc ttctgcctca tctggctgga aagcagaatg
     7081 agcgtccttt aagagaaggt cttaagggag aagaactgaa tgtgatcatt ggatccatcc
     7141 cctacgaggg cgaggagatt aaggaagccg taaagctgat ggttttatct cttctccatg
     7201 agaaatacgg cattaaggaa aaagacttct tccgcgcaga gtttgaattg gttcctgccg
     7261 caaaggcaag agatatcgga ctggacagaa gccttgtggg cgcctatgga caggatgacc
     7321 gggtatgcgc ttatacagcc cttaccgcag agattgagac aaagaaaccg gcccatacca
     7381 cagtaaccat tcttacagat aaggaagaaa tcggctctga gggaaatacg ggtcttaatt
     7441 ccgactatgt gcttcactat atcgaagatc tggcagagct ggagcatacg ccggtacggg
     7501 atattttaag agcctccctg tgcctttctt ctgatgtaaa tgccgcttat gacccaacct
     7561 ttgcagatgt atttgaaagc cgcaatgcaa gctacatcaa caaaggctgt gttttgacaa
     7621 aatatacagg tgcaagaggc aaatccgctt ccaatgacgc aagtgcagag actatggcaa
     7681 aagtgatcgc tattatggag gaggcaggcg tattctggca ggcaggagag ttaggagccg
     7741 ttgatgtggg aggcggcggc accattgcaa agtatgtagc ccatatggat gtggataccg
     7801 tagacttggg tgtgccgatc ctttccatgc attcaccctt tgagctggct tccaagctgg
     7861 atgtgtacaa tacctacaag gcatttaagg cattttatgc ataaaaggct tttttttcaa
     7921 cgtcttgtat ggtataacta tgtacagcgc ctgcctgaga gcaggcgaaa atagtatgat
     7981 agaaaaggga aaccattatg agaaaaatcg cattattttg tctgtgcggc cttacggcag
     8041 cttctattct gactggctgc tccggcagtg cagacggcaa cgtaaatttg ggaaccgtta
     8101 cactttccga gtacaaggga gttactgtta atattgaggc tcctgaggtt acggatgagg
     8161 aagtggatac aaaggtagag actatcctgg cacagaaccc caacgaggta gaggtagacc
     8221 gggccgccaa ggagggcgat attgtaaata tcgacttcaa gggaacaaag gacggagaag
     8281 cctttgaagg cggttctgca gaggatcagg atctggagct gggttccggt aagatgatcg
     8341 agggctttga aagcggcctg atcggtgcaa agaagggtga gacaaagaca ttaaatctta
     8401 ccttcccgga ggactacaga gaggaatctc tggcaggaca gccggttgta tttgaggtaa
     8461 ctgtaaactc cgtaaaggag gagcaggagg cagagctgaa cgatgatttc gtaaagaggg
     8521 tgtcaaagga agagtatcag accgtagacg cttataagga aagcatccgt gaggatctgc
     8581 tggatcagaa gcagtccgca gcagaacagg atatggaaga gggagccctt cagagcgtga
     8641 tcagcagttc agaatttaag ctttccagaa acggcctttc tgtccgctac aatcagatgg
     8701 taaaagaata caccaaccag gctaagatgt acggcatgag cttttcacag atggctcagg
     8761 ccaacggcat gaacgagccg gccatgaagg agtatatcta cgattccgta gaggaagctg
     8821 ccaagagaga actgactgta gaagccatcg cagcaaatga gggactggat cagatcacag
     8881 atgaggacag agaactcttt gctcaggaaa acggtatgtc aaaggatacc ctgatcaata
     8941 tgtacggaca ggagcaggca gacgagtatg tgcttcagga taaggtactc tactatctgg
     9001 cttccaatgc tgtcaatgaa gcagaaaatc cggcaaaggt ttctgagcgg gttctggaga
     9061 agactgaggc ttccccagag gcagaataaa aaggaataag agaagcgtcg ggagcgtttc
     9121 ataggttata ccacagaaaa aggaggacta tgcatgcaga tcttaaaaga caggatccgt
     9181 aaagacggaa aaatcaaggg cggtaatgtt ctgaaggtag acagtttttt aaatcatcag
     9241 atggatatca agctttttgg cgaaattgga aaggaattta agcgcagatt tgcggatgct
     9301 gaggtaaata agatcctcac catcgaggca tccggtattg gaattgcatg tatcgtagcc
     9361 cagtattttg atgtgccggt ggtatttgca aagaaaaacc agaccaaaaa tatcgcagga
     9421 gaggttcaca ccaccaaggt agaatctttt acccatggca gggtgtacga tatcattgtg
     9481 gcaaaggaat ttttaggaaa aggcgataag gttcttatta tcgatgattt cctggcaaat
     9541 ggcaaggcgc tggagggact gatcgcactg gtaagagatt ccggagccga gttggtagga
     9601 gccggtattg taatcgaaaa gggcttccag gtaggcggag atctgatccg ttccgagggg
     9661 atccgtctgg agtcccttgc gatcgtagac agcatggacg aggaaacaaa taccattgta
     9721 ttcagggatg atgaataaaa tgaaggcatt attttttgac atagacggaa cccttctttc
     9781 tgaaaagacg cggcagattc ctgagagcgc aaaggaagcc cttaaggaag ccagacgcag
     9841 aggccatctt gtatttatca atacgggccg cgtgtactgc cacctgtctc agatccgctc
     9901 tgaggtagag gcagacggct ttctctgcgg ctgcggcacc tgtgtggtag ttcaggatca
     9961 gattctctac cgccacgcga ttccctatga acgggggctt cagatgaagc gcgatattga
    10021 tgcgtgcggc ctggacggca tcctggaggg aacagaaggg tgctatatgc ataaaacgcc
    10081 ttcccgcatc ccaagagttg aggagcttaa gaatgcgatc cgggcaggcg gctgtgtcat
    10141 tccccatgac tgggaggatg aaggctatgt gtttgacaag atttatcttg gatccgatga
    10201 aaacagccgt cccaaagaac tttttggacg aatgaaggac atggagatca ttgaccgggg
    10261 aaatggcttt tatgaatgtg tgccaagagg tcattcaaag gcaaccgcta ttgaccaggt
    10321 gttaaagcac tgcggcattt ccctggaaga cgcctatgtg ttcggagaca gcagcaatga
    10381 cctgtccatg ttccgatatg cttccaactg tgtccttatg ggccatcaca gcccggtgct
    10441 ggagccctat gcttcctttg ttacaaagac agtagaggag gacgggattg cgtatgccat
    10501 gaaggagctg gggattattt agccccggtt cggaaatgga aagcaggata taacggatga
    10561 aacagaaaac ctggatcata agtgccctga tgtgtgccat ggttatcatg gtatgcctcg
    10621 gggtactgca ccttttaaag gaaatgacag aaaacaatgc accctcaggg actgtggctg
    10681 aggaaagcac agaccataca gaaccggaaa tcgattttgc agaggaagag gaaacgaaag
    10741 aggacatagc agagacgggt ccggaggaag aggaaagcga tgttcccgta gagagggaag
    10801 aaggatacta tggatggaag caggagcttc cccagatttt tccttctgtt ccggaggagg
    10861 ttccctatga gccgcccagg ctgatccttg cgaccgatct tcactatcag agcgcccagg
    10921 cagatgacgg cggagccgcc tttcagcttt ttgtggagcg gggagacggc aaggtggtgg
    10981 aatatctgcc ggagctttta gaagcattta tggatcaggt catagaggaa catccctcgg
    11041 ctctggtttt aagcggcgat atcac
//
LOCUS       DBMDUH010000087        10984 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_2959_length_10984_cov_6.781770, whole genome shotgun sequence.
ACCESSION   DBMDUH010000087 DBMDUH010000000
VERSION     DBMDUH010000087.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 10984)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10984)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10984
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2959_length_10984_cov_6.781770"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            378..773
                     /locus_tag="ACZPCG_08390"
     CDS             378..773
                     /locus_tag="ACZPCG_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009534456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285117.1"
                     /translation="MKKRNLFAAAAVSLILATGCGAQPSSQPVSSAEETVSTQVSESA
                     DTPSAEASSADTDVKTSSITGTIDEIKDFMFNITDSEGTSFVLSFDATPEGLSDVKNG
                     DTVTVTYTGELSEVDAFTGTVISVKKAEK"
     gene            complement(870..1691)
                     /locus_tag="ACZPCG_08395"
     CDS             complement(870..1691)
                     /locus_tag="ACZPCG_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HIR1285118.1"
                     /translation="MKRRFGVMAMAAVLSAALAVPAYAGTWKYVNDQWKYQRGANKFA
                     YNEWIQDKGKYYYLDNDGYVTTGWQQIEGQWYHMDSTGAMETGWFKDSDSGKWYYLYP
                     SGVMAVNTVIDGRTIGTDGVWVPEEGAVEPSDSADLSTQQLVQNMEGISTKGYSILTS
                     ARTGGGERWDNAVRLKGKGSHVICNTNGEYRLLYGSFGPSSSQFDSGLMARVTVYGDN
                     DQVLYTSSDIHHNEKVTYFGVDVSGQNQIRVEVSLSKDNGYDDPVILFNGLALYK"
     gene            complement(1830..2339)
                     /gene="nrdG"
                     /locus_tag="ACZPCG_08400"
     CDS             complement(1830..2339)
                     /gene="nrdG"
                     /locus_tag="ACZPCG_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HIR1285119.1"
                     /translation="MNYGNIKECDIANGPGVRVSLFVSGCRHHCKGCFNAETWDFGYG
                     KPFTEETGREILRLLEPSYIQGFTVLGGEPFEPENQPAVAELLKQIRMVYPQKDIWCY
                     SGYLYDSDMQPGGRVYTEHTQEIFKCLDVLVDGEFVEAEKDLTLVFRGSSNQRLLKLK
                     DGIACGNWP"
     gene            complement(2372..4561)
                     /gene="nrdD"
                     /locus_tag="ACZPCG_08405"
     CDS             complement(2372..4561)
                     /gene="nrdD"
                     /locus_tag="ACZPCG_08405"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HIR1285120.1"
                     /translation="MSVDVKTNVIKRNGEEVDFNSQKIINAVHKANEEVDRLHQMNDY
                     QIIAIADNIANKVNESTHAVNVEDIQDMVETGIMAMRGYEVAQKYVRYRYKRELTRKS
                     NTTDNGILSLLDNINEEVNQENSNKNPVINSTQRDYMAGEVSKDLSRRVLLPEEIVRA
                     HEEGIIHFHDTDYFAQREHNCDLINLEDMLQNGTVISETLIEKPHSFFTACNVTTQIV
                     AQVASNQYGGQSFTLAHLAPFVDISRQKIRKNVIEERKECGESLDEAIISKVTERRLR
                     DEVRSGIQTIQYQLITLMTCNGQAPFVTVFMYLDEVPEGQTRDDLAMIIEEVLVQRMQ
                     GVKNEKGVWITPAFPKLIYVLDEDNISEESKYWGLTELAAKCTAKRMVPDYISAKIMK
                     ELKQGDVYPCMGCRSFLTVEDSQRNADGSHKYYGRFNQGVVTINLVDVACSSEGDMDE
                     FWKILDERLELCHRALRCRHERLLGTISDVAPILWQNGALARLKKGEKIDRLLFDGYS
                     TISLGYAGLYEMCMRMLGKSHTDPEARPFALKVMKRLNDKCREWKEAEHISYSVYGTP
                     MESTTYKFSKCLQKRFGIIPGVTDKNYITNSYHVHVSEQIDAFSKLKFEAEFQKLSPG
                     GAVSYVEVPNMQNNIPAVLSVMKFIYNNIMYAELNTKSDYCECCGYDGEMAIKEDESG
                     KLIWECPNCGNQDQNHMFVARRTCGYIGTQFWNQGRTQEIRDRVLHL"
     gene            complement(4803..6194)
                     /gene="hydA"
                     /locus_tag="ACZPCG_08410"
     CDS             complement(4803..6194)
                     /gene="hydA"
                     /locus_tag="ACZPCG_08410"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009244204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HIR1285121.1"
                     /translation="MKVFVKNGTIVSETGQYQGDLCIENGVISQIGTHLEPTSEEEAQ
                     VIDAAGKYVLPGGVDVHTHMDLLAGSSRAVDDFYDGTVAAACGGTTSIVDHMAFGPAG
                     CPLHHQLNEYHGLADHKAVIDYGFHGTCQHVNEEILNELEDMMKDGVPSVKVYLTYDF
                     KIDDDGVLQVLKKMKQLGGVTAFHCENHAVVQFLRGKLVSEGNTAPIYHAKSRPNLAE
                     AEAVRRVLYLAKLAGDAPVYIVHLSCKESLEAVKEARAEGQKNIFVETCTQYLVLTEE
                     RYLDEDGLKYIMSPPLRTKEDCEALWKGIAEGEIQAVATDHCPFNYGKEKQLGKDDFT
                     KCPNGAPGVEERMIILFSEGVMTGKISLEKLVEIACTQPSRIYGLYPKKGCLQPGADG
                     DLLILDPSKEWVLTHSHMHSAVDYTAYEGMKIRGSIELVMQRGNIIVKDNHFLGKQGN
                     GHFIFRKPLSLCE"
     gene            complement(6289..6843)
                     /locus_tag="ACZPCG_08415"
     CDS             complement(6289..6843)
                     /locus_tag="ACZPCG_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036094 - small molecule binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3H domain-containing protein"
                     /protein_id="HIR1285122.1"
                     /translation="MNGTERREQILSILKNSEKPVPGVELARILHVSRQVIVQDMALL
                     RANGVEILSTNRGYVTAEEHSCSRVFKVIHTEKQVEEELNLYVDFGGKVEDVFVYHKV
                     YGVIRADMNIKSRMDVKRYLQDISEGKSTQLMKLTSNYHYHTISAEREEILDMIQEEL
                     EKRGFLAKLQDYEPVDFWGTSEEA"
     gene            complement(6861..7715)
                     /gene="nadC"
                     /locus_tag="ACZPCG_08420"
     CDS             complement(6861..7715)
                     /gene="nadC"
                     /locus_tag="ACZPCG_08420"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HIR1285123.1"
                     /translation="MYDRVTLKLNVDPLILSALREDITSEDVSANSIMKKPQAGEVDL
                     ICKQDGIICGLQVFERVFALLDDSTRVEFFAEDGEEVKNGQLLAKVYGDIRVLLCGER
                     TALNYLQRMSGIATYTHSVAALLKGTKTKLLDTRKTTPNNRIFEKYSVRVGGGNNHRY
                     NLSDGVLLKDNHIGAAGGVAEAVKMAKEYAPFVRKIEVEVETLDMVKEAVEAGADIIM
                     LDNMSHEQMKEAMEYIDGRAEVEVSGNVTKENISRLTDLGVDYISSGALTHSAPILDI
                     SLKNLHAV"
     gene            complement(7700..8992)
                     /locus_tag="ACZPCG_08425"
     CDS             complement(7700..8992)
                     /locus_tag="ACZPCG_08425"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012739658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HIR1285124.1"
                     /translation="MKTDILIVGSGCSGLYCALNLPESRQITVITKAGAEENDSYLAQ
                     GGMCMLKDESDYQGFFDDTMKAGHYENDEASVEIMIRSSRSVVEDLLQFGADFQRDEN
                     GELVFTREGAHSEKRIIFHKDVTGKEITSTLLAQTKRRSNIMLLEHTSMIDIIEENNI
                     CYGAVVQRQDGSLETIEADYTVMATGGIGGLYRHSTNFRHLTGDALAVAVRHKIALKD
                     ISYVQIHPTTLYADEERSFLISESVRGEGAVLRDKNGERFVNELLPRDLLTKAILEQM
                     EKDGTEFVWEDLRTIPENELNRHFPNIVEHCRKIGYDVTKECIPVVPAQHYFMGGIKV
                     SHESRTSMEQLYAVGETACNGVHGKNRLASNSLLESLVFAKRAALDMTEHLGEVKRNS
                     YLFERLDISQYEDFSGLYKTYHKLVKNAIKGEERCTIG"
     gene            complement(8999..9904)
                     /gene="nadA"
                     /locus_tag="ACZPCG_08430"
     CDS             complement(8999..9904)
                     /gene="nadA"
                     /locus_tag="ACZPCG_08430"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009262816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HIR1285125.1"
                     /translation="MSITEEIKHLKKEKNAVILAHYYVADEVQEIADYIGDSYYLSKA
                     AQKTAADIIVFCGVKFMGESAAILNPEKKVLMPDMTADCAMAHMVQLEKIREMRETYE
                     DLAVVCYINSTAEIKTYSDVCVTSANAVKIVRNLPNQNIFFIPDGNLGRYVAEQVPEK
                     HIIFNDGYCPIHAAMSAEAVKREKEKHPEAKFLVHPECTGDLLKLADYIGSTSGIIDF
                     AAEDDGKEYIIGTESGVLYELKKQNPDKTFYTLTENQICTDMKLITPEKVLQVLKEEI
                     NEVKVPEKVRIQAGAPLEKMLMLAR"
     gene            complement(10122..10202)
                     /locus_tag="ACZPCG_08435"
     tRNA            complement(10122..10202)
                     /locus_tag="ACZPCG_08435"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10166..10168),aa:Leu,seq:tag)
     gene            complement(10255..10339)
                     /locus_tag="ACZPCG_08440"
     tRNA            complement(10255..10339)
                     /locus_tag="ACZPCG_08440"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10303..10305),aa:Leu,seq:taa)
     gene            complement(10350..10422)
                     /locus_tag="ACZPCG_08445"
     tRNA            complement(10350..10422)
                     /locus_tag="ACZPCG_08445"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10387..10389),aa:Val,seq:tac)
     gene            complement(10462..10535)
                     /locus_tag="ACZPCG_08450"
     tRNA            complement(10462..10535)
                     /locus_tag="ACZPCG_08450"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10499..10501),aa:Asp,seq:gtc)
     gene            complement(10577..10650)
                     /locus_tag="ACZPCG_08455"
     tRNA            complement(10577..10650)
                     /locus_tag="ACZPCG_08455"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10614..10616),aa:Ile,seq:cat)
     gene            complement(10691..10763)
                     /locus_tag="ACZPCG_08460"
     tRNA            complement(10691..10763)
                     /locus_tag="ACZPCG_08460"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10728..10730),aa:Thr,seq:tgt)
     gene            complement(10787..10858)
                     /locus_tag="ACZPCG_08465"
     tRNA            complement(10787..10858)
                     /locus_tag="ACZPCG_08465"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10823..10825),aa:Glu,seq:ttc)
     gene            complement(10893..10965)
                     /locus_tag="ACZPCG_08470"
     tRNA            complement(10893..10965)
                     /locus_tag="ACZPCG_08470"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10930..10932),aa:Asn,seq:gtt)
BASE COUNT         2711 a         3025 c         2077 g         3171 t
ORIGIN      
        1 ctacactcaa cgctttgtgc acaaaaaaca aaaaagccaa ccgaaacggc tggctttttg
       61 agctcatcaa cgttaaatgt tttatttatt aagttgttac gattgtaaca cccattatca
      121 ttcatgtcaa tatccattca ttatttcatt attttttgta tattatcaca attttttaat
      181 gttctaaaat ttccaatcga gcatataatt tatattttat atggtttttt ccatcttccc
      241 ccctcacatt acattttcac ttttttgttt aaaatgcaca ataaaaaaat aactttctct
      301 ctgcaacttt ctcaccccgc ctcccgtcta agcatatgta ctgaaaaata tattgcatat
      361 acttatggag gtttattatg aaaaaaagaa atttattcgc agcagcagcc gtttccctta
      421 ttttggcaac cggatgcggg gcccagccct cttctcagcc ggtttcttcc gcagaagaaa
      481 ccgtttccac acaagtttcc gagtccgcag acaccccttc tgctgaagcc tcctccgctg
      541 atacggatgt caagacctca tccattacag gcaccattga tgaaatcaag gattttatgt
      601 ttaacataac cgacagcgag ggcacatcct ttgtcctgtc ctttgacgca acgcctgaag
      661 ggctctctga tgtaaaaaac ggtgacacgg ttactgttac ctacaccgga gaattaagtg
      721 aggttgatgc gtttaccgga actgttatct ctgttaaaaa ggccgaaaaa taaatctata
      781 aactgaaaac tgccgcagcc agaacgcgca tccaggacat atgcctgaag aatcgccttc
      841 tgactgcggc agtttattat cttatgtttc tatttgtata aagccagccc attaaacaga
      901 atcacaggat catcataccc gttatctttt gacaaggaaa cctctactct gatctggttc
      961 tgtccggaga catctactcc aaagtaggtt accttctcat tgtgatgaat atccgaagag
     1021 gtataaagca cctgatcgtt atccccataa acagtcactc tggccataag gccgctgtca
     1081 aactgagaag acgatgggcc gaaggaaccg tataaaagcc gatattcgcc gttggtattg
     1141 cagattacat ggcttccttt tcccttcagc cggaccgcat tatcccagcg ctccccgcct
     1201 ccggttctgg ccgatgtgag gatcgaatat ccctttgtgg aaattccttc catattttga
     1261 accaactgct gggtagacag atcagcgctg tcagagggct ctaccgcccc ttcctccggt
     1321 acccacactc cgtctgttcc aatggttctt ccatcaatta ctgtattaac cgccattaca
     1381 ccgcttggat acagatagta ccattttccg ctgtcactgt ctttaaacca gccggtttcc
     1441 atggctcctg tgctgtccat atgataccac tggccctcta tctgctgcca gcccgttgtc
     1501 acataaccgt cattgtccag ataataatac tttcccttat cctgaatcca ttcattgtag
     1561 gcaaacttat tcgcaccccg ctgatacttc cactggtcat ttacatattt ccatgtaccc
     1621 gcataagccg gaactgccaa agctgcgctc aatacagcgg ccattgccat tactccaaaa
     1681 cgtctcttca tactgcttct cctttccaga aagacagcac cgattacaat acaggggcgc
     1741 tgccaggttg attttcatta taattcaatt tgatgaatta tgcaagaaag ccttggctgc
     1801 acccctgtta tttcataaaa atttcatact tacggccagt ttccgcaggc aattccgtct
     1861 ttcagcttca gaagcctctg attgctgctc ccccgaaata ccagggtcag atccttctct
     1921 gcttccacaa attcaccgtc caccagcaca tccagacatt taaaaatctc ctgcgtatgt
     1981 tctgtgtata ccctgcctcc cggctgcata tctgaatcat aaagataacc gctgtagcac
     2041 cagatatcct tctgcggata taccattcgg atctgcttta aaagctctgc cacagccggc
     2101 tggttctccg gctcaaaagg ttctccgccc aaaacggtga aaccctggat ataggacggt
     2161 tccagcagcc tgagaatctc tctgccggtt tcctctgtaa acggttttcc gtatccaaaa
     2221 tcccaggttt ctgcattaaa gcagccttta cagtgatgcc tgcagccgga tacaaagagg
     2281 ctgacccgga cccctggacc gtttgcaata tcacattcct taatatttcc gtaattcatc
     2341 ctgctctcct ttcggaccgg cccaaaaacg cttacagatg aagcaccctg tctctgatct
     2401 cctgagtccg tccctggttc cagaactggg ttccgatata accgcaggtt ctgcgggcta
     2461 caaacatatg gttctgatcc tgatttccgc agttggggca ctcccagata agctttccgg
     2521 actcatcctc cttgattgcc atttctccgt catagccgca gcattcgcag taatcgctct
     2581 tcgtattcag ctccgcatac atgatattat tataaataaa cttcatcaca gataaaacag
     2641 ccggaatgtt gttctgcata tttgggactt ccacatagct taccgcaccg cccggcgaca
     2701 gcttctggaa ctctgcctca aatttcagct tgctgaatgc atcaatctgc tccgatacat
     2761 ggacatgata gctgtttgta atataatttt tatccgttac gcccggaatg attccgaaac
     2821 gcttctggag gcacttggaa aacttatagg tcgtggattc cataggcgta ccgtatacgg
     2881 aatagctgat atgctctgcc tccttccact ccctgcactt gtcattgaga cgcttcatta
     2941 ccttcagtgc aaagggtctg gcctccggat cggtatggga cttgcccagc atcctcatac
     3001 acatttcata caggcccgca tagcccagag aaatggtaga atagccgtca aagagcagac
     3061 ggtcaatctt ttctcccttt ttcaggcggg ccagagcgcc gttctgccag agaatcggcg
     3121 ccacatcgga aatcgtgccc agcaggcgct catgacggca tctgagggct ctgtggcaca
     3181 gctccagacg ttcatccaga atcttccaga attcatccat atcgccctcg ctggaacagg
     3241 ctacatccac cagattaatg gtcactacgc cctgattgaa acggccgtag tatttgtggc
     3301 ttccatctgc atttctctgg gaatcctcaa ccgtcaaaaa ggagcggcag cccatgcagg
     3361 ggtatacatc cccctgtttt aattccttca tgatctttgc agaaatatag tccggaacca
     3421 tccgcttggc tgtacatttt gcagccagct ctgtgagtcc ccagtatttg gattcctctg
     3481 aaatgttatc ctcatccagc acatagatca gcttagggaa tgcaggcgta atccagacgc
     3541 ccttttcatt tttcacaccc tgcatcctct gtaccagaac ctcttcgatg atcatggcga
     3601 ggtcatccct tgtctgtccc tcaggcacct catccagata cataaatacg gttacaaagg
     3661 gagcctgtcc attacaggtc atcagggtaa tgagctggta ctgaatggtc tggattccgc
     3721 tgcgtacctc atctctcagc ctgcgctccg ttacctttga gataattgcc tcatctaagg
     3781 attctccgca ctctttgcgc tcctcaatca catttttacg gatcttctga cggctgatat
     3841 ccacaaaggg agccagatgg gccagcgtaa aggactggcc gccgtactgg ttgctggcaa
     3901 cctgagcaac gatctgtgtg gttacattgc aggccgtaaa aaagctgtga ggcttttcga
     3961 tcagtgtttc actgataacc gtgccgttct gaagcatatc ctccagattg ataaggtcac
     4021 agttgtgctc cctctgtgca aaataatccg tatcatggaa atggataatg ccctcctcat
     4081 gggctctcac gatctcctcc ggcagcagca cacggcggga taaatccttg ctcacctctc
     4141 ctgccatata atctctctga gtggaattga taaccggatt cttattggaa ttctcctgat
     4201 tgacctcttc attgatattg tctaacaggg acaggatccc gttatctgtg gtgttggact
     4261 tacgggtcag ctctctctta tagcggtagc gtacatactt ctgtgccacc tcatagcctc
     4321 tcatggccat gatcccggtt tccaccatat cctgaatatc ctctacattc actgcatgag
     4381 tggattcatt caccttattt gcaatattat ctgcaatggc gataatctga tagtcattca
     4441 tctgatgaag ccggtctacc tcttcgtttg ctttgtgaac cgcatttatg atcttctggg
     4501 aattaaagtc tacctcttcc ccatttctct taatcacatt tgtcttaacg tcaacactca
     4561 tcccgatccc tcgcttatat ctgtcatttt atctcataat aataagcacc cccggtttat
     4621 tcctttctca tctggaaaaa ccactagata tagtgccgtg tactatacta ctacaaaatc
     4681 atgtgaaatg aaagaggaat attggaaaat attaaattca gacaacagcc gtttttctcc
     4741 ctaataaatt gccaaaagcg ctgccagacc atcctttcat ggcggcagcg cccggttttt
     4801 ccttattcac acagcgaaag cggttttctg aaaataaaat ggccgtttcc ctgttttccc
     4861 aaaaaatggt tatcttttac aattatattt cccctctgca ttaccagttc aatggaacct
     4921 cttatcttca ttccttcata ggcagtatag tccactgcac tgtgcatatg gctgtgagtc
     4981 agcacccatt cctttgaagg gtctaaaatc agcaggtcac catccgctcc gggctgcagg
     5041 cagccttttt tcggatacag gccgtagatt cttgaaggct gtgtacaggc aatttccacc
     5101 agcttttcca gactgatctt tcccgtcata acaccctcgg aaaacagaat gatcatgcgt
     5161 tcttccactc ccggcgctcc attggggcat ttggtaaagt catcctttcc aagctgcttt
     5221 tcctttccgt aattaaacgg acagtgatcc gtagccactg cttggatctc accctctgca
     5281 atgcccttcc acagtgcttc acagtcctcc tttgtgcgca gaggcggtga cattatatat
     5341 tttaatccat cctcatccag ataccgctcc tctgtcagaa ccaggtactg tgtacaggtc
     5401 tccacaaaaa tgtttttctg tccctctgct ctggcctctt ttactgcctc cagactttcc
     5461 ttacaggata agtggacaat atacaccggc gcatctccag ccagctttgc cagataaagc
     5521 acccggcgca ctgcctctgc ctctgccaga ttggggcggc tcttggcgtg gtaaatgggt
     5581 gccgtattgc cctctgatac cagctttcct cttaagaact gcacaaccgc atggttttca
     5641 cagtgaaatg cagtcacgcc ccccagctgc ttcattttct ttaatacctg aagcactccg
     5701 tcgtcatcaa tcttaaaatc ataggtcaga tataccttta cgctgggaac gccgtccttc
     5761 atcatgtcct ctaactcgtt caggatctct tcgttcacat gctggcaggt accgtgaaac
     5821 ccatagtcga tcactgcctt atgatctgcc agcccgtgat attcattcag ctgatgatga
     5881 agagggcagc cggccggccc aaaggccata tgatccacaa tagaggtggt gcctccgcag
     5941 gctgcagcta cggtgccatc gtaaaaatca tccacagccc ggctggatcc ggctaaaagg
     6001 tccatatggg tatgaacatc tacgcctccg ggaagcacat attttcccgc cgcgtcaatc
     6061 acctgggctt cttcttccga cgtcggctct aaatgggtgc caatctgtga aataactccg
     6121 ttttctatgc agagatcgcc ctgatactgc cccgtttctg atacaatcgt tccattttta
     6181 acaaaaacct tcatctgtaa ctcctccgtg ttttcattca tgggaatatt ataccaaacc
     6241 acaggcccgt gggctagtcc caagtacttt gagtttttac atttcacact aagcttcttc
     6301 tgatgttccc cagaaatcca ccggttcata atcctgaagc tttgccagaa acccccgttt
     6361 ttccagctcc tcctgtatca tatccagaat ctcctcacgc tcggcagaaa tggtgtgata
     6421 atggtaattc gatgtgagct tcatcagctg agtggatttt ccctcagaaa tatcctgcag
     6481 ataccgcttt acatccattc tggatttgat attcatatct gcccggatga caccgtaaac
     6541 cttgtggtat acgaatacat cctccacctt cccgccaaag tccacataca gattcagttc
     6601 ctcttcaacc tgcttctccg tatggatcac cttaaatacg cggctgcagc tgtgctcctc
     6661 tgctgtcaca tatccgcggt tcgtagagag gatttccact ccattggcac gcagaagcgc
     6721 catatcctgc acaattacct gacggctcac atgaaggatt ctggccagct ccacccccgg
     6781 aaccggtttt tcgctgtttt tcagtatact cagaatctgt tcccgtcttt ctgtaccgtt
     6841 cattcctgtc cctctcttct ttagactgcg tgaagatttt tcagtgaaat atccagaatc
     6901 ggtgcagaat gcgtcaatgc tccgctggaa atataatcca caccaagatc ggtcaggcgt
     6961 gaaatgttct cttttgtcac atttccggac acctccacct cagctcgtcc gtcaatatat
     7021 tccatggctt ccttcatctg ttcatggctc atgttatcca gcataataat atctgcacct
     7081 gcctcaacgg cctcctttac catgtccaga gtctctacct ctacctcaat tttccgcaca
     7141 aagggagcgt actcctttgc cattttcaca gcctctgcca cgcctcctgc tgctccgata
     7201 tggttgtcct tcagcaatac gccgtcggaa agattatatc tgtggttatt tccaccgcct
     7261 acacgaacag aatatttctc aaaaatgcgg ttattcggtg ttgtctttct tgtatccaga
     7321 agcttcgtct tagtcccctt taataacgct gctactgaat gggtatatgt ggcaatgccg
     7381 ctcattctct gaagataatt gagagccgtg cgctccccac acaaaagtac gcggatatcg
     7441 ccgtaaacct ttgcaagcag ctggccgttc tttacttcct ctccatcctc tgcaaaaaat
     7501 tcaactcttg tgctgtcatc taaaagggca aatacacgtt caaagacctg caggccgcag
     7561 ataatcccat cctgcttgca gatcagatcc acctctccgg cctgaggctt tttcataata
     7621 ctgtttgccg acacatcctc gcttgtaatg tcctcacgca gtgcgcttaa gatcaatgga
     7681 tccacattta attttaaagt tacccgatcg tacatctttc ttctccttta atcgcatttt
     7741 tcaccagttt atgatacgtt ttatataatc cggaaaaatc ctcatactgg cttatatcca
     7801 gcctttcaaa cagatagctg tttcttttta cctctcccaa atgctccgtc atatcgagag
     7861 ccgcccgctt tgcaaataca agactttcca aaagagaatt gctggccaga cgattttttc
     7921 catggacacc gttgcaggca gtctcgccta ccgcataaag ctgttccatg gacgttcggc
     7981 tttcatggct gactttaatt cctcccataa aatagtgctg agccggaact actggtatac
     8041 attcctttgt tacatcatag cctattttcc tgcaatgttc aacaatattg gggaaatggc
     8101 ggttcagctc attttcgggt atggtgcgca gatcctccca gacaaactct gttccatctt
     8161 tttccatctg ttctaaaatg gcttttgtca gcagatccct tggaagaagt tcattgacaa
     8221 agcgctctcc gtttttatcc cgcagcaccg ccccctctcc ccgcacagac tcagagatca
     8281 ggaaactcct ctcctcatcc gcgtacagtg tggtcgggtg aatctgtaca taagaaatat
     8341 ccttaagcgc aatcttgtgg cggactgcga ctgcaagtgc gtctcctgtc agatggcgga
     8401 aattggtaga atgtcggtac aatccgccga tccctcctgt tgccataacc gtgtaatccg
     8461 cctcaatggt ttccaggctt ccatcctgtc tctgaaccac agccccatag catatattat
     8521 tttcctcaat aatatcgatc atagacgtat gctccagcag cataatgttg gatcgtctct
     8581 ttgtctgggc taaaagtgtg ctggtaatct cttttcctgt cacatccttg tgaaatatga
     8641 tccgtttctc tgaatgggct ccctctctgg taaacaccag ctccccattc tcatcccttt
     8701 ggaaatctgc cccaaactgc agcagatcct ccaccacaga cctggaggaa cgaatcataa
     8761 tctctacgga ggcttcatca ttctcataat gtcctgcctt cattgtatca tcaaaaaatc
     8821 cctgataatc ggattcatcc ttcagcatac acattccacc ctgagccaga taggaatcat
     8881 tttcctctgc tcctgccttt gtgatcaccg taatctgtct gctttccgga aggttcagtg
     8941 cacagtaaag acctgaacag ccgcttccta cgattaaaat atctgtcttc atttcacatt
     9001 atcgggccag catcagcatt ttttccagcg gtgctcctgc ctgaatcctc actttctccg
     9061 gaaccttaac ctcattgatt tcttccttta aaacctgtag aaccttttca ggcgtaatca
     9121 gcttcatatc tgtgcagatc tgattttctg taagcgtata aaacgtttta tccggattct
     9181 gttttttcag ttcatacaat acccctgatt ctgtaccgat aatgtattcc tttccatcat
     9241 cctctgctgc aaaatcaatg atccccgatg tgcttcctat gtaatctgcc agcttcagca
     9301 aatccccggt acactcagga tgaaccaaaa atttggcttc cggatgtttt tccttttccc
     9361 tttttacggc ttcagcagac atagccgcat ggatgggaca ataaccgtca ttgaaaatga
     9421 tatgcttctc cggaacctgt tctgccacat accttcccag atttccatcc ggaataaaga
     9481 aaatattctg attcggcaga ttccttacaa ttttcaccgc attggccgag gttacacaca
     9541 catccgaata tgtcttaatt tctgccgtgg aattgatata acagaccacc gccagatcct
     9601 catatgtttc tctcatctcg cggatttttt ccagctgtac catatgggcc atggcacagt
     9661 ctgccgtcat atccggcatc agaaccttct tttccgggtt caaaatcgca gcgctttctc
     9721 ccataaattt cactccgcaa aatacaatga tatctgcagc agtcttctga gctgccttgc
     9781 tcaaatagta agaatctcca atataatccg caatctcctg cacctcatct gcaacataat
     9841 aatgggccag gattactgca ttcttttcct tttttaaatg cttgatttct tctgttattg
     9901 acatgtgttc ctccataatg ctcattcctc tgaaatattc cacacaacat caggcattat
     9961 agcactcttt ttgtcatatg acaagacagt agtcatttca gttgtgccgt ataaacctat
    10021 attcacaaaa ctgatggtaa attcggtcaa aacaatcgga aatccagcgc atgaaaaaaa
    10081 cgctgcatcg ccatgaatca tgacaataca acgtttctta atgcgggcaa caggacttga
    10141 acctgcacgt cttggacacc agaacctaaa tctggcgcgt ctgccaattc cgccatgccc
    10201 gcggatataa aaacagccgc acaagatacc ttctcctgtg tgaccgaata aaaatgcggg
    10261 tggccggact tgaaccggca cggatgttaa gtccgaggga ttttaagtcc cttgtgtctg
    10321 cctattccac cacacccgca aaacacaaat ggggcctata gggctcgaac ctatgaccct
    10381 ctgcttgtaa ggcagatgct ctcccagctg agctaagacc ccaaattgca ttaaattgta
    10441 atgcatctta tcagatgcaa acgacccgga acgggttcga accgtcgacc tccgccgtga
    10501 cagggcggcg ctctaaccag ctgagccacc gggccatatg ttttaatata gttcttacaa
    10561 ctttagctag gaagaatgga ccttcgggga cttgaaccca ggaccgaccg gttatgagcc
    10621 ggttgctcta accaactgag ctaaaggtcc ggaactatat cttttaaatt atctactgct
    10681 tgagcagtaa agccgatgat cggactcgaa ccgataacct gctgattaca aatcagctgc
    10741 tctgccaatt gagccacatc ggcatatctt gaacatgatg ttcaaatgac cccaacgaga
    10801 ttcgaactcg tgttaccgcc gtgaaagggc gatgtcttaa ccgcttgacc atggggccat
    10861 attcagtagt agtcgccatg acttccacta agctccccga gtaggactcg aacctacgac
    10921 ccaacggtta acagccgttt gctctaccga ctgagctatc gaggaataca aattacgctg
    10981 actg
//
LOCUS       DBMDUH010000088        10923 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_2978_length_10923_cov_5.685456, whole genome shotgun sequence.
ACCESSION   DBMDUH010000088 DBMDUH010000000
VERSION     DBMDUH010000088.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 10923)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10923)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10923
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2978_length_10923_cov_5.685456"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..81)
                     /locus_tag="ACZPCG_08475"
     CDS             complement(<1..81)
                     /locus_tag="ACZPCG_08475"
                     /inference="COORDINATES: protein motif:HMM:NF013491.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285126.1"
                     /translation="MNERAILYRKMEGIPAEWGTAVSVQAM"
     gene            246..530
                     /locus_tag="ACZPCG_08480"
     CDS             246..530
                     /locus_tag="ACZPCG_08480"
                     /inference="COORDINATES: protein motif:HMM:NF023580.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HIR1285127.1"
                     /translation="MEWRDDHWHSYPVFQYQADGKTVIIQHNVSPTSYEIGDQVELRY
                     NQKNPEQYYIPHASGGNWMFLFGGLFFFLMGFGFTALIIKVWLLPLILRI"
     gene            complement(658..2061)
                     /gene="rlmD"
                     /locus_tag="ACZPCG_08485"
     CDS             complement(658..2061)
                     /gene="rlmD"
                     /locus_tag="ACZPCG_08485"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003500049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HIR1285128.1"
                     /translation="MKKGDICEAVVEKIDFPNKGILHVDGERVIVKNAVPGQKVRFVI
                     NKKRKGKCEGRLLEVVEHSPIEQPDRACPHGGICGGCLYQGLPYEEQLKIKAEQVKGL
                     IDEVCDSYEFQGIKGSPIYEGYRNKMEFSFGDEYKDGPLALGMHRRGSFYDIVTTPEC
                     RIVHPDFCQILEATLEYFSSRKIGYYRKLQHTGYLRHLLVRRAVKTGEILTALVTSGQ
                     TEVFAGDTQGDGRAQEQELLKGWMEALKELPLEGSFAGILHIRNDSLADVVQSDETQV
                     LYGKDYFYEELLGLTFRISPFSFFQTNSLGAEVLYETARSYIGETKDKVVFDLYSGTG
                     TIAQITSPVADKVVGVEIVEEAVEAARKNAAFNGLENCEFIAGDVLKVIDELTDRPDI
                     IILDPPRDGIHPKAIGKIIEFGVDRMVYISCKPTSLARDLVPLQEGGYKVEKVCCVDM
                     FPFTSGIETVCLLSHVK"
     gene            complement(2168..2509)
                     /locus_tag="ACZPCG_08490"
     CDS             complement(2168..2509)
                     /locus_tag="ACZPCG_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HIR1285129.1"
                     /translation="MNRFDRDRLDAMGKEALSRVEELMNTSRINELMHKREEDEKKRN
                     CILWILAIIGAVAAVAGIAYAVYRFFTPDYLEDFEDDFDDDFDDDFFEDEESVKKAEE
                     KKEEKEEDQEA"
     gene            complement(2686..5046)
                     /locus_tag="ACZPCG_08495"
     CDS             complement(2686..5046)
                     /locus_tag="ACZPCG_08495"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003509233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HIR1285130.1"
                     /translation="MSIYDTLNPMQKEAVLHTEGPLLILAGAGSGKTRVLTHRIAYLI
                     EEKEVNPWNILAITFTNKAAGEMRERVDSLVGFGAESIWVSTFHSTCVRILRRYIEHL
                     GYTTSFSIYDSDDQKTLMKQVFKTLDIDTKQYKERSVLGIISSAKDKLISPEEFLLNA
                     GQDFRQKKVGEIYREYQNQLKKNNALDFDDLIVKTVELFQNDSQILDYYQERFRYIMV
                     DEYQDTNTAQFKLISLLASKYGNLCVVGDDDQSIYRFRGADIENILSFEQTFPGARVI
                     KLEQNYRSTQNILDAANGVIRHNRGRKEKSLWTANGTGDEILFKQFDDARDEADYIAR
                     QIRDSEFSYQDQAVLYRTNAQSRLLEERCIFYNVPYRLVGGVNFYQRKEIKDILAYLK
                     TVANGVDDLSVLRIINVPKRGIGATSMGRVTMFASEHGMSLYSALREARMVPGLGKAV
                     EKIGRFISQMECFRAMAQSEEYSIQDLIEAILEETGYEEELKAEGEIEAETRMENIQE
                     LINKAAAYEEDSQHPSLDEFLEQVALVADIDNVDSSEDRVTLMTLHSAKGLEFPKVYL
                     AGMEDGLFPGMMAVSSDDPTDMEEERRLCYVGITRAKKELVITAARKRMTHGETRYCK
                     PSRFVEEIPAELLKEERLEPVLGSARGADSFGGASGRFGAGEEGLPWNQPGSGTVSTF
                     GKGYNAYASKTTGFGTANAGFKGGKGGLGSGQSFGTNPGFGKSFTVQKAASLDYKEGD
                     RVHHDRFGDGTVKEIVDGARDYEVTVEFDTGGQRKMMACFAKLKKI"
     gene            5210..5833
                     /locus_tag="ACZPCG_08500"
     CDS             5210..5833
                     /locus_tag="ACZPCG_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HIR1285131.1"
                     /translation="MKSNAEQLSGLMEFLNSQTHVTPEEIPNIDLYMDQVTTFMEEHL
                     KDSKRYEEDKVLTKTMINNYAKNNLLPPPVKKKYSRDHMLMLIFIYYFKSLLSFHDIE
                     TFFGPITSRHFSSGSQSSGLSLEDIYREVFSLEEREMEHLKADVSAKFSRAMDTFKDC
                     PAREGEQEYLRLFSFICELSFDVYLKMRLIEKIADQLREDEPPLKKK"
     gene            complement(5949..8627)
                     /gene="xdh"
                     /locus_tag="ACZPCG_08505"
     CDS             complement(5949..8627)
                     /gene="xdh"
                     /locus_tag="ACZPCG_08505"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007712428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0046415 - urate metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HIR1285132.1"
                     /translation="MSSYCKVWINGQEVEADEERKLMDVLRNDLRLKSVKDGCSEGAC
                     GTCTVLIDGKATKSCVQKMGRLAGKHILTVEGLSQREKDVYVYAFGETGAVQCGFCIP
                     GMVICAKGLLDVNPDPSRVEIAYAIRNNYCRCTGYKKIIDAIQLAAKMLREDIAPAEQ
                     EKMIRVGSRVHRIDAEGKVLGTGKYVDDLDKVDFPEMIYGSAVRSKYPRAIVKAIHTE
                     KAKALPGVVCVLTADDVPGKNKVGHLKQDWDTMIAVGDTTRYLGDAICLVAAETPEIL
                     EQAKALVEVEYEKLKPVTCPAEAMEEGAPLVHSTGNILSHEHLVRGNADEVIAASKYK
                     VTRHYETPWTEHAFLEPECAVSMPFDGGVLIYSTDQGTYDTRHETSILLGLPPEKVIV
                     ENMLVGGGFGGKEDVTVQHQAALIAYITKRAVKVKLTRKESILVHPKRHPMSIDLTTA
                     CDENGRLTAMKAVVVADTGAYASLGGPVLQRACTHAAGPYNYQVIDIDGKAVYTNNPP
                     AGAFRGFGVTQTCFATEMNINLLAEMCGMDPWQFRYLNAIRPGQVLPNGQIADPSTGL
                     AETLEAVKDIYYSNPYVGIGCAMKNAGVGVGLPDTGRVRLIVEGGKVHIHSGASCIGQ
                     GVGTVLVQMLAESAGISMDEIEYHRPNTSMAPDSGTTSGSRQTLVTGEACRRAAKDLR
                     RALFEATGRSYEATEMPSAYLSNVMVAKESPETAAVTFTAEEVALLNGKEFYGEYLAK
                     TDKMGADVEYPVSHVAYGYATQLCILKEDGTIEKMVGAHDVGKVVNPKSAEGQVEGGI
                     VMGCGYALTEQYPLKDCVPTAKYGTLGLFRADKAPEVESIIVEKPGVDVAFGAIGIGE
                     ITSIPTAPAVAGAYYKWNQIFETELPLKNTPYAKKK"
     gene            complement(8611..8850)
                     /locus_tag="ACZPCG_08510"
     CDS             complement(8611..8850)
                     /locus_tag="ACZPCG_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HIR1285133.1"
                     /translation="MAREKTLKAVYTFHTTTEAMKMEQCAREHGIPGRLIPVPRQISA
                     GCGMAWAAPAEAKARTDEEIRLSDVEVEGVYELLL"
     gene            complement(9003..>10923)
                     /locus_tag="ACZPCG_08515"
     CDS             complement(9003..>10923)
                     /locus_tag="ACZPCG_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1285134.1"
                     /translation="FNIEKIVDAGIWPVTMATTMLKPGGYQRLEQIGRIFEAKQAPVF
                     EGVDAAKVRALVEGARIDKHHVKAVKPLPSRKVNRPVPLVDCFIAPCQEGCPIYQDIT
                     RYLQLAGEGKHEEALKVILNKNPLPFITGTICAHNCMSKCTRNFYETAVNIRRTKLEC
                     AEGGYEAVMKELKAPAVSSDKKAAVVGGGPAGLAAAFFLAKGGMKVTVFEKEEKMGGV
                     VRNVIPGFRIPDSAIDNDVEIVKAMGAELVTGREIQSVEELKKDYDYVVLALGASEPG
                     VLKLEAGEPVNALEFLAQFKATDGKVDLGKNVVVIGGGNTAMDTARAAKRNAGVEKVS
                     LVYRRTRRYMPADEEELVMAIEDGVEFAELLSPVRQENGVLICKKMVLGDIDASGRRG
                     VVETDEIVEVPADTVIAAVGEKVPSGFYEANGIALDSRKRPQVNQDTLETSLKGVYAV
                     GDGLFGPATVVEGIRDGKKAAEAIIGRDLSEDIFKMSDEEVITYRKGNLAEEDESRTD
                     SGRCLSCNSYCENCMEVCPNRANIALVVPGMEKHQIIHVDYMCNECGNCRSFCPWDSA
                     PYLDKFTLFANEADMADSTNQGFAVLDAEKGACKVRLQGNEFAYQTGTADERVPEGIA
                     KIIETVIRDYSYLIL"
BASE COUNT         2594 a         3090 c         2169 g         3070 t
ORIGIN      
        1 catggcttgt acacttacag cagtacccca ttcggcagga ataccttcca tcttgcggta
       61 aagaatggcg cgttcgttca tccaactacg gaatacagca aggagggaaa cgtatggcgg
      121 gttatatttg tgcccttgca atcagcttgt ttgcagttat aacagggatc attttcatga
      181 tttatccgct gtatcacaaa catgtctgca acgaaccggc aacggcaaac gtaattgata
      241 ttcatatgga gtggagggat gatcactggc actcctatcc agtatttcaa tatcaggccg
      301 atggaaaaac ggtcataata cagcacaatg tcagccccac ctcctatgag attggggatc
      361 aggtcgaact gcggtataac cagaaaaacc cagagcagta ttacattccc catgcgtccg
      421 gtgggaattg gatgttcctg tttggcgggt tgtttttctt tttgatgggg tttggcttta
      481 ccgcactgat cataaaggta tggctgcttc cgctgatttt gagaatttaa actaatgtaa
      541 gatcagacaa aacagttcac ttttaaaaaa cagcaaccat ttgtcaaccg ctcaaaaaag
      601 ggttgacaaa tggttgacaa atcgacaatt atgtattgca gctttccctg taaacactta
      661 ttttacgtgg gaaaggaggc acacagtttc tatcccgctc gtaaatggaa acatatccac
      721 gcagcacacc ttttcaacct tatatccgcc ctcctgaaga ggcaccagat ctctggccag
      781 gctggtaggc ttgcagctga tataaaccat ccgatctacc ccaaattcaa tgatctttcc
      841 aatagccttt gggtgaatgc catcccttgg cggatccagg ataataatat ccgggcggtc
      901 ggtaagttca tcaataacct ttaatacgtc tcctgcaata aattcacagt tttccagacc
      961 attgaacgcc gcattttttc tggcagcttc cactgcctcc tccacgatct ccacacctac
     1021 taccttgtca gccaccggtg atgtgatctg ggcaatggtt ccggttccgc tgtagaggtc
     1081 aaatactacc ttgtcctttg tctctcctat gtagcttctg gctgtttcat agagaacctc
     1141 ggcccccagt gagttggtct ggaagaagga aaaggggctg atgcggaagg taaggcccaa
     1201 aagctcctca taaaaatagt cctttccata aagtacctgt gtttcatcgc tctgaaccac
     1261 atctgccaga ctgtcattgc ggatatggag aatccctgca aagcttccct ctaaaggcag
     1321 ttccttaagc gcctccatcc atccttttaa cagttcctgc tcctgagccc gtccgtctcc
     1381 ctgagtatcc ccggcaaaaa cctctgtctg gcctgaggtt acaagagcgg taaggatttc
     1441 tcctgttttt acagccctgc gcaccagcag atgacgcaga tagcctgtat gctgcagctt
     1501 tctgtaatat ccgatctttc gactggaaaa atattccaga gtcgcctcca gaatctggca
     1561 gaaatcagga tgtacgattc gacactctgg tgtagtcaca atatcataaa agcttcctct
     1621 gcggtgcatt ccaagagcca gaggcccatc cttatactca tccccaaagg aaaattccat
     1681 cttgttgcgg tagccttcgt atatgggact tcccttaatt ccctgaaatt cgtaggaatc
     1741 acagacctca tcaatcagcc cctttacctg ctctgcctta atctttagct gctcctcata
     1801 gggaagcccc tgatacaggc agccgccgca gatgccgcca tggggacagg ccctatccgg
     1861 ctgctcaatc ggtgagtgct ccaccacctc caacaggcgg ccttcacact ttccctttct
     1921 ttttttattg atcacaaaac gaaccttctg accgggaact gcattcttta caatgacccg
     1981 ctctccatcc acatgaagaa tccctttatt tggaaaatca attttttcta caactgcttc
     2041 gcagatatct ccctttttca atacgtttac tccttacttc ttatgtctgt ttcaaagaaa
     2101 cagccggaaa cggatccggc tcttttcgcc ttttgttccc ggctgttacg cgctgtttgg
     2161 aggataatta tgcttcctga tcctcttctt tttcttcctt cttttcctct gccttcttta
     2221 cagactcctc atcctcgaag aaatcatcgt cgaaatcgtc gtcgaaatca tcctcaaaat
     2281 cttccagata atcaggtgta aagaaacggt atacggcata ggcaataccg gctacggcag
     2341 ctacagctcc gataatagcc agaatccaga gaatgcagtt tctctttttt tcatcctctt
     2401 ctcttttgtg catcagttcg ttgatccggc tggtattcat cagctcctct acacggctta
     2461 atgcttcttt tcccatggca tccagtctgt ctctgtcaaa acgattcatg gcatatccgt
     2521 cctttcttgc aataacggga agccgggatt tggaacggtt catccgcacg caaccccatt
     2581 ttagctgccc ggcgcttcgc tataacttat tatagcatgg aatcattgtt tttactatcc
     2641 catttctttt cagtttttcc aattttctgc cagatatctg aacatttata tttttttcag
     2701 ctttgcaaag caggccatca tttttctctg accacctgta tcaaattcta ctgtcacctc
     2761 gtaatctctg gccccgtcca caatttcctt tactgtgccg tctccgaacc ggtcgtggtg
     2821 aacccggtcg ccctctttat aatccagtga cgctgccttt tgaactgtaa atgactttcc
     2881 aaagccgggg ttcgtcccaa agctctgacc gctgccaagg ccgcctttac cacctttaaa
     2941 gcccgcattt gccgttccaa aacctgtcgt ttttgaagca taggcattgt acccctttcc
     3001 aaaggtgctt accgtgccgc ttcccggctg attccaggga agtccctctt ctccagctcc
     3061 aaatcgtcct gacgctccgc caaaactatc tgcccctctc gcacttccaa gcaccggctc
     3121 cagcctctcc tccttaagaa gctctgccgg gatttcctcc acaaaacggc tgggtttaca
     3181 gtagcgcgtt tctccgtggg tcatccgctt tctggccgca gtaatcacaa gctccttttt
     3241 ggctctggta atccccacat agcacagcct ccgctcttcc tccatatccg tggggtcatc
     3301 cgaagaaacg gccatcattc ccggaaacag tccgtcctcc atacctgcca gatatacttt
     3361 gggaaactcc agtcccttgg cgctgtgaag ggtcataagc gttaccctgt cttctgagga
     3421 atctacatta tcaatgtcag ccaccagagc tacctgctct aaaaattcat ccagagaagg
     3481 atgctgtgaa tcctcctcat aggcggctgc cttattgatc agctcctgga tattttccat
     3541 acgggtctcc gcttcaatct caccctctgc cttcagctcc tcctcatatc ccgtttcctc
     3601 cagaatcgcc tcaatcagat cctgaatgga atattcttcc gactgggcca ttgcccgaaa
     3661 gcactccatc tgagaaataa aacgaccgat tttctcaact gcttttccaa gccccggcac
     3721 cattctcgct tccctaagag cactgtaaag gctcatgcca tgttctgagg caaacatcgt
     3781 cacccttccc atggatgttg ctccaattcc tctcttcggc acattaataa ttctcagaac
     3841 ggaaagatcg tctacaccat tggcaaccgt ctttaaataa gccagaatat ccttgatttc
     3901 ctttctctgg tagaaattca cgcctcccac cagccggtat ggcacattgt aaaaaataca
     3961 ccgctcctct aacagacggg actgggcatt ggtacggtaa agtaccgcct gatcctgata
     4021 ggaaaactca ctgtctctga tctgacgggc aatataatcc gcctcatccc ttgcatcatc
     4081 aaactgttta aacaggatct catctcctgt tccgttggct gtccacaggg atttttcctt
     4141 tcttcctctg ttgtggcgga tcacaccgtt agccgcatcc agaatattct gggtagaccg
     4201 gtaattctgc tccagcttaa tcactctggc gccgggaaat gtctgctcaa agcttaaaat
     4261 attttcaata tccgcccctc tgaaccggta aatggactgg tcatcatcac ctaccacaca
     4321 cagattccca tatttggatg ccagaaggct gatcagctta aactgagccg tattggtatc
     4381 ctgatactca tctaccataa tatatcggaa ccgttcctgg tagtaatcca gaatctggct
     4441 gtcattttga aataactcca ctgtttttac aatcagatca tcaaaatcca gagcattgtt
     4501 ctttttcagc tgattctgat attccctgta aatctcccct acctttttct ggcggaaatc
     4561 ctgtcctgca ttcagaagaa attcctccgg agagatcagc ttatccttgg cagaggaaat
     4621 aattcccagt accgaacgtt ccttatactg ctttgtatca atatccagcg tcttaaacac
     4681 ctgcttcatc agcgtcttct ggtcgtcgct gtcatagatg gaaaagctgg tggtatatcc
     4741 cagatgttca atatagcgcc gcagaatcct cacacaggta gaatgaaagg tgctgaccca
     4801 gatgctttcc gctccaaagc ccaccagaga atccactctc tcacgcatct ctcccgccgc
     4861 cttattcgta aaggtaatag ccaggatatt ccagggattg acctcttttt cctcgatcag
     4921 gtatgcaatc cggtgggtca gtacccgggt cttccccgat ccggctcccg ccagaataag
     4981 gagaggacct tcggtgtgaa gcactgcctc cttctgcatg ggatttaatg tatcataaat
     5041 actcataaat gtaccgcctt tttatttctg tcattttcgc ctccaaacag tatagcataa
     5101 gtgatgaccc aggaaaagtc ctttcgcgat tcctcttgcc atctggtttt taatactata
     5161 taatagatac agcaatacaa catcatgtgt caaaccgagg tgaatataga tgaaatcgaa
     5221 cgctgaacag ctctcaggcc ttatggaatt tctaaactcc cagactcatg taacgcctga
     5281 ggaaattccg aacattgacc tgtatatgga tcaggtaact acctttatgg aggagcattt
     5341 aaaggactcc aagcgctatg aagaggacaa ggtcttaaca aagaccatga tcaacaatta
     5401 cgccaaaaac aaccttctgc ctcctccggt aaagaaaaaa tattccagag atcatatgct
     5461 gatgctgatc tttatctact attttaaaag tcttctgtcc ttccatgata tcgaaacctt
     5521 tttcggcccc attacgtcca gacatttttc ttccggaagc cagtcctccg ggctttctct
     5581 ggaagatatt taccgtgagg tcttttcttt ggaggagcgt gagatggagc acttaaaggc
     5641 agatgtttcc gccaaatttt cccgtgctat ggacaccttt aaggactgtc ccgccaggga
     5701 aggagaacag gagtatctgc gtcttttttc ctttatatgc gaactgtcct ttgatgtata
     5761 tctgaaaatg cgcctgatcg aaaaaattgc agatcagctc cgtgaggacg aaccgcctct
     5821 gaaaaagaaa taaaccacag ccgaaaaccg ccccggtctt tgacggatca ttcatccgac
     5881 agcaagaccg gggcggtttt cctatcatca catattcaca tcatttatct gcatcatatt
     5941 gcaggatttc acttcttttt tgcatatggc gtattcttaa ggggcagttc tgtctcaaaa
     6001 atctgattcc acttatagta agcacctgcc actgcgggag ctgtaggaat agaggtaatc
     6061 tcaccgatac cgatggctcc gaaggctaca tccactcccg gtttttcaac aattatagac
     6121 tctacctcag gagccttatc tgcacggaac agtcccagag ttccatactt ggctgtaggt
     6181 acgcagtctt ttaagggata ctgctctgtc agcgcatagc cgcagcccat aacaattccg
     6241 ccttctacct gcccctctgc actctttgga tttacaactt ttcccacatc atgggcgccc
     6301 accatttttt caatggttcc gtcctctttt aagatgcaca gctgggtcgc atagccgtaa
     6361 gccacatggg ataccggata ctctacatca gcgcccatct tatctgtctt tgccagatat
     6421 tcgccgtaga attcttttcc atttaacaga gccacttctt cagccgtaaa ggtaacagcc
     6481 gccgtctccg ggctttcctt tgccaccatt acattggaca ggtatgcgct tggcatctcc
     6541 gttgcttcat aactgcgtcc ggttgcctca aaaagagccc ttcttaaatc cttggcagcc
     6601 cttctgcagg cttctcctgt taccagtgtc tgtctggaac ctgaggtagt tcccgaatca
     6661 ggagccatgg aggtatttgg acggtgatac tcaatctcat ccatactgat gcctgcactt
     6721 tctgccagca tctgtaccag aaccgttcca acgccctgtc cgatacagga ggcgccagaa
     6781 tggatatgaa cctttccgcc ctctacgatc agacgtacac ggcctgtatc cggaagtccc
     6841 actcctacgc cggcattctt catggcacag ccgattccca cataaggatt gctgtagtaa
     6901 atatccttta ctgcctccag ggtctctgcc agaccggtag aaggatctgc aatctgtcca
     6961 ttcggcagca cctgtcccgg acggattgca ttcaggtaac ggaactgcca gggatccata
     7021 ccgcacatct ctgccagaag attgatattc atctccgttg caaaacaggt ctgtgtcacg
     7081 ccaaagcctc tgaaagctcc cgcaggcgga ttgttggtgt aaactgcctt tccgtcaatg
     7141 tcgatcacct gataattata cggtcctgct gcatgggtac aggctctctg gagcaccggc
     7201 cctccaagag atgcgtaagc tcccgtatca gccactacca cagccttcat agctgtaaga
     7261 cggccgtttt catcacaggc agttgtcagg tcaatgctca tgggatgacg ctttggatgc
     7321 accagaatac tttccttacg ggtcagcttt accttaacgg ccctctttgt aatataggca
     7381 atcagcgctg cctgatgctg aaccgttacg tcctccttgc cgccgaagcc gccgcctacc
     7441 agcatatttt ctacgattac cttttccgga ggaaggccca aaaggataga agtttcatga
     7501 cgggtatcgt aggtaccctg atcggtagaa taaatcagca cgccgccgtc aaatggcatg
     7561 gaaactgcac attcaggctc caaaaatgca tgctctgtcc agggagtctc ataatggcgg
     7621 gttactttgt atttggatgc agcaatcact tcatctgcat ttcccctcac cagatgctca
     7681 tggcttaaaa tatttcctgt ggaatgtaca aggggagctc cctcttccat agcctctgcc
     7741 ggacaggtca caggttttaa cttctcatac tccacctcta ccagagcctt tgcctgctcc
     7801 agaatctccg gtgtctcagc cgctacgagg cagattgcgt ctcccagata ccgtgtggta
     7861 tctcccactg cgatcatggt atcccagtcc tgctttaagt gacctacctt gttctttccg
     7921 ggaacgtcat cagctgtcag cacgcagaca acgccgggaa gggcttttgc cttctccgta
     7981 tgaatagcct ttacaatggc tctgggatat ttggagcgta cagcacttcc atagatcatt
     8041 tccggaaaat ccaccttatc cagatcatct acatattttc ctgttcccag tacctttccc
     8101 tcagcatcga tccggtgaac cctgcttccc accctgatca tcttctcctg ctccgccgga
     8161 gcgatatcct cccgcagcat cttagctgcc agctggatcg catctataat ctttttatat
     8221 ccggtacacc ggcagtagtt attgcggatc gcataggcaa tctccacacg gctgggatcg
     8281 ggatttacat ccagaagccc tttggcgcag atcaccattc ccggaataca gaaaccgcac
     8341 tgaaccgctc ccgtttctcc aaaggcatat acatacacat ccttttctct ctggcttaaa
     8401 ccctctaccg ttaaaatgtg ttttcctgcc agacggccca ttttctgtac acaggacttg
     8461 gttgcttttc cgtcgatcag caccgtacag gtaccacagg cgccctcaga gcagccatcc
     8521 tttactgatt tcaaacgcag atcatttcta agtacgtcca tcagtttccg ctcttcatct
     8581 gcttccactt cctgtccgtt gatccatact ttacaataag agctcataaa ctccctccac
     8641 ttctacatct gacaaacgaa tttcttcatc tgttcttgct tttgcttcag ccggagccgc
     8701 ccatgccatc ccgcagcctg cgctgatctg cctgggaaca gggatcagac ggccgggaat
     8761 cccatgctcc ctggcacact gctccatttt catggcttct gttgtagtgt gaaatgtata
     8821 cactgccttt aatgtctttt cccttgccat ggcttctcct tttttcgtta taccctctcg
     8881 aatataacgt tactttttta acaaatgcct tgtgtatcag gcccggaggg aaatcctcct
     8941 cccctccggg cctgctttca gcttttcatc gaagttctgt cctttttccc aaatatcacg
     9001 aattacagaa tcagatagct gtaatcccgg attacagttt caatgatctt tgcaattccc
     9061 tcaggaactc tctcatctgc tgttccggtc tggtaagcaa actcatttcc ctgaagccgt
     9121 accttacatg ctcccttttc agcatctaac acagcaaagc cctgattggt gctgtctgcc
     9181 atgtcggcct catttgcaaa gagagtaaac ttgtcaagat atggagcgct gtcccatgga
     9241 cagaagctgc ggcagtttcc gcattcatta cacatatagt ccacatggat aatctgatgc
     9301 ttctccatgc ccggaactac aagggcaata tttgcacggt tggggcagac ctccatacag
     9361 ttctcacagt agctgttgca ggaaaggcat cttccgctgt ctgttctgct ttcatcctct
     9421 tctgccagat ttcccttacg gtacgtgata acctcttcat cagacatctt aaagatatcc
     9481 tcggaaagat ctcttccgat aatagcctct gcagccttct ttccgtcccg gatgccttct
     9541 actacagtag caggtccgaa gaggccgtct cccactgcat acacgccctt aagggaggtt
     9601 tccagtgtat cctgatttac ctgaggacgc tttctggaat ccagggcaat accgtttgct
     9661 tcatagaagc cggaaggaac cttctcgcct actgctgcga ttacagtatc tgccggaacc
     9721 tctacgatct catctgtctc aacaacgcct ctgcggccgc tggcatcaat atcgcccaga
     9781 accatctttt tacagatgag tacaccgttt tcctgacgga ccggagagag aagctctgca
     9841 aactctacgc cgtcctcgat tgccattacc agctcttcct catccgcagg catatagcgt
     9901 ctggttcttc tgtataccag agaaaccttt tcaacgcctg cgttgcgctt tgcagctctt
     9961 gctgtatcca tagctgtatt tccgccgccg atcactacta cgtttttgcc caggtcaacc
    10021 tttccgtcgg ttgccttaaa ctgtgctaaa aattcaaggg cattaaccgg ctcgcctgcc
    10081 tctaacttca gtacgcccgg ctcagaagct cccagagcca gaaccacata gtcgtaatcc
    10141 ttcttaagct cttcaacaga ctggatctcc cttccggtta ccagctctgc tcccatagcc
    10201 ttaacgatct ctacatcatt gtcaatggcg ctgtcaggaa tacggaagcc cgggattacg
    10261 ttgcggacaa caccgcccat tttctcttcc ttttcaaata cagttacctt cattccgccc
    10321 ttggctaaga agaacgctgc tgccagacct gcagggcctc ctcctactac agccgccttc
    10381 ttatcagaag aaacagccgg agccttaagc tctttcatca ctgcctcata gccgccctcg
    10441 gcgcactcta acttggttct gcggatattg acagctgtct catagaagtt tctggtacat
    10501 ttgctcatac agttatgagc acagatcgta cctgtgataa aaggaagagg attcttattt
    10561 aagataacct tcagcgcttc ctcatgcttg ccctctcctg ccagctgcag ataccgggta
    10621 atatcctgat agatgggaca gccctcctga cagggtgcaa taaagcagtc taccagcgga
    10681 accggacggt ttacctttct ggacggaaga ggctttacag cctttacatg atgcttatcg
    10741 attcttgcac cctctaccag agctcttacc tttgcggcat ccacgccctc aaatacagga
    10801 gcctgctttg cctcaaagat tcttccgatc tgctccagtc tctgatagcc tccgggcttt
    10861 aacatggtgg ttgccatggt tacaggccag atacccgcat ccacgatctt ttcaatatta
    10921 aag
//
LOCUS       DBMDUH010000089        10843 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_3004_length_10843_cov_5.637684, whole genome shotgun sequence.
ACCESSION   DBMDUH010000089 DBMDUH010000000
VERSION     DBMDUH010000089.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 10843)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10843)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10843
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3004_length_10843_cov_5.637684"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="ACZPCG_08520"
     CDS             complement(<1..204)
                     /locus_tag="ACZPCG_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285135.1"
                     /translation="MRIKKEKTQQRGCIRRILAGLCLAGQLGLAAGLCPEIGQIQRSG
                     IIQEKGVMYTAASGGAAASYEAAA"
     gene            363..1283
                     /locus_tag="ACZPCG_08525"
     CDS             363..1283
                     /locus_tag="ACZPCG_08525"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR1285136.1"
                     /translation="MIKRSLSYLISSADAGRTIEEFLRTRGYSHRLIVHLRNTPLGLT
                     IKGETAYTTHILKEKELLCVTLIEEESSEHIIPSPLPLSIVYEDEDILVINKAPGVPI
                     HPSQGHFEHTLANAVAWYYQSKGEPIIYRAINRLDRDTSGLLIIAKHMLSACILSDQM
                     VKRQIRREYRAVVCGNLPPEETITVPIARVPGSTIMRQTDFENGEYACTHFTKLSYNS
                     KNDLSYISLRLDTGRTHQIRVHMAHIGHPLPGDFLYHPDYRFINRQPLHSFRLDFIHP
                     LTKEPMTFFAPLPDDMASLISEGAQTPASV"
     gene            complement(1246..2763)
                     /locus_tag="ACZPCG_08530"
     CDS             complement(1246..2763)
                     /locus_tag="ACZPCG_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HIR1285137.1"
                     /translation="MITLPEAFKKRMKELFKDEYEAFEASYEKERVQGLRFNSLKSVE
                     GRKIGWEKEALPGLAALMENRLGTSLTPIPWVKEGYYYEAQARPGRHPFHEAGVYYIQ
                     EPSAMAVTELLDPQPGERILDLCAAPGGKSSHIASRLKGKGFLLSNEIHPARAKILSQ
                     NMERMGVSNGVVVNEEPSRLAAVFPEFFDGIVVDAPCSGEGMFRKDEEAVEQWSEEHV
                     KMCAARQEQILAQAARMIKPGGRIVYSTCTFAPEENEGTVLNFLKCHEDFYLEQTEGC
                     EKFSHGKPEWAGFGDGEEKSHEEEAAIYHLERTFRLLPHCLEGEGHFIAVIRRREGIE
                     RKEAKRTYPAYLDRRRNRDVFSLLEAFLKESLADGSSYLNRAEYVMFGDQLYLLPGEM
                     PDLKGLKVLRPGLHMGTVKKNRFEPSHALALSLLPEEAKRVYRMEADGEETAAYLRGE
                     ALSCRMESWPGNEKGWVLMCAEDWSLGWSKLSGGCLKNHYPKGLRRMQASAHPQK"
     gene            complement(2843..3175)
                     /locus_tag="ACZPCG_08535"
     CDS             complement(2843..3175)
                     /locus_tag="ACZPCG_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6465 family protein"
                     /protein_id="HIR1285138.1"
                     /translation="MATRKSTRAVSAQAKKAVKEAAEEVKAVAKKAPVKKPAARKAAE
                     PSAAVHFQYNGRDIAAKEVLAAAMEDFKKNHPDEEIKEFELYIVAEENAAYYVVNGIP
                     SDDYKIVL"
     gene            complement(3412..5265)
                     /locus_tag="ACZPCG_08540"
     CDS             complement(3412..5265)
                     /locus_tag="ACZPCG_08540"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HIR1285139.1"
                     /translation="MSEEKKKRPVSRSREAFKARRKERARAKKTKKGSVKAVGAGMAA
                     AVVISIGGYIYQAGAYTDTYFPNTTVNGMEVGGKTVGEVKEMIASEAASYRLFLSMRQ
                     GETAQIFGNEIGLLAEFDGNLEEILRQQDPYSWPLYLLRGPEYDIKALISYDETQLAA
                     AVSKLSQSDPEAAEAPADAVISEYEEGKGYSILPESEGARLNQERTLKAAEEAICGLL
                     PELDLDQAGCYEAPAVRSDDPVLRKRLEQMNRFAFMSVTYTFGSRREILDGKTISQWI
                     SYDENDQPVIDEEQLAAYVKALGSEYNTAYTKRKFKTSYGPEVEVAGVYGWRIDGAGE
                     KARLKEEILAGEPVEREPVYLQTAASREGNDYGTTYAEVNLTAQHLFFYKDGKKILES
                     DFVSGNQSKGYTTPPGLFSLTYKQRDAVLKGEGYASPVKFWMPFNGGIGFHDASWRNT
                     FGGTIYKKNGSHGCVNMPYEAAKTLYENVYKGMPVICYNLEGTERSTAGSASGRAEPP
                     ASASPTAVPETVPQTVPQTPPETEPPVQTAPLQPAETVPETPQETQPPKPSEEIYGSG
                     STQSTQNPETGAAGPGVTESTGKPSSNEADPGADTPKTTSPNDVIIQPVGR"
     gene            complement(5297..6655)
                     /locus_tag="ACZPCG_08545"
     CDS             complement(5297..6655)
                     /locus_tag="ACZPCG_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007712388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1285140.1"
                     /translation="MKLENNLDTDRIRELVWKLAFPSMLAQFVSVLYSIVDRMYIGNI
                     PEIGGQALAAVGVCGPVVTLITSFASWVGIGGAPIMSIRLGQKNEKAAQNVVANCFVL
                     LAVMAAVLMTVMYLFKNQLLTVFGAGSAIFPYADEYLTWYLSGTAFALLSTGMNQFII
                     CQGFAKVGMMTVLLGAVSNIILDPVFIFVLDLGVKGAAIATVLSQLASCVYVLFFLLG
                     KRPLVRITFGGYQKELMARVLVLGLSPFLITASDSGLIIIMNMLIRHYGSYEMGDVLL
                     TCNTIVQSFMLMITMPLGGITGGTQTILGYNYGAGRPDRIRKAQKHIIGLGMVFTAVM
                     FVIAMTLSAQFAEIFTNDRFFIETSVRAIRISVLAIVPLAIQYEVVDGFTGMGAAALA
                     ISQSMFRKAVYLAAMLILPALAGIENVFYAEPAADVIASAVAVGVYFVFSGRVLGDGK
                     RF"
     gene            complement(6677..7831)
                     /gene="ytvI"
                     /locus_tag="ACZPCG_08550"
     CDS             complement(6677..7831)
                     /gene="ytvI"
                     /locus_tag="ACZPCG_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HIR1285141.1"
                     /translation="MEEYKNSREKTEPQWRYILRLILNIMIPASGWFFLCFLGPKLLG
                     FFMPFVVGWIIAMIANPLVRFLERRLKLVRRHSSIVIVAGALALVIGLLYLAVSRMLH
                     FIWSFIQDLPVLYAGIEGDVQSSLGRLEGIFDFMPETMRDAWMTLGDNLGAYIGKLAE
                     SIASPTMEAAGTVAKGIPAALVYFVVIILSAYFFIADRDRILSVIRSHMPEWTGRYSN
                     HLKKESRRLIGGYFMAQFKIMAVVWLILTAGFFILGVSYGPLWALLIALLDFLPVFGT
                     GTALIPWGIIKVLGGEYAFAAGLILIYVLTQVIRQLVQPKLVGDTMGLNPLLTLFLLY
                     LGFKIRGLAGMILAVPIGLFIASLYRFGAFKGMTDSARELAEEISRFRGK"
     gene            complement(7880..9082)
                     /locus_tag="ACZPCG_08555"
     CDS             complement(7880..9082)
                     /locus_tag="ACZPCG_08555"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006779976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR1285142.1"
                     /translation="MISEKMKPLVNNNSAIRAMFEEGKRLAAIYGAENVYDFSLGNPN
                     VPAPEAVNEAICRIVNEEPSVMVHGYMSNAGFEDVREAIAQSLNRRFGTKFHQGNILM
                     TVGAASGLNVILKTILNPQDQVVTFAPYFVEYGNYVRNYDGILEVVPADTETFQPDLE
                     EFSRRITDRTKAVIINTPNNPTGVIYSEAVLTRLGEILTEKEKKFGTQIVLISDEPYR
                     ELAYDGAEVPYVTKFYHNTVICYSYSKSLSLPGERIGYLVIPDELTDSKEVFNAAVIA
                     NRVMGAVNAPSLMQRVVMRCVDETADIEAYDRNRNLLYRGLREAGFECVKPQGAFYMF
                     VKSPEPDEKKFCEACKKEHILVVPGSSFACPGYVRISYCVSFDQIRRSLPAFKRVAEG
                     YGLKGLED"
     gene            complement(9288..>10843)
                     /locus_tag="ACZPCG_08560"
     CDS             complement(9288..>10843)
                     /locus_tag="ACZPCG_08560"
                     /inference="COORDINATES: protein motif:HMM:NF039456.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6449 domain-containing protein"
                     /protein_id="HIR1285143.1"
                     /translation="LGGMAGGWFFWTLQSQVKWGLFGTAAGILLVHCVVEIIYHFDFK
                     KLFSHKMQLALCLGTGMLFFCSFRYDWYGYDSYVPSQEEIASASVDLSIDYSFAANMP
                     RVMRQGDTRRLIYESAGDFIKEKMELTDFESVIALAEAASREAAGYREEKLNRWEAQI
                     SLNEVSMSVNHYSSKDASAISVIGGADGPTSVFVAGKEEEEAYDRYFSQILIRYRLKN
                     GRQVSRRYHMDLEDVLETYENIYNNETYKKGLYPILSQQPEEMGYVSYQGNGNHSLFN
                     TMEEDAIKELLGAYQTDLKGQKLKDRMREDPVGALQFAEKELCLYIQERWGKNDGYIS
                     EYQRDLIKMLLGPETLKDSYNEMPSADEMERYMENHLDQLCRWPVYPSFTNTIQVLEK
                     MGVEPGAYFAAEHVSRISVDVSSIVNGEEYGDGLPEGEALKQIQTENPFYQKDGTLVF
                     TEQEQIELLMGALRDRELADMNGMREPEQPARYDLYVTLTLDESRVSAEFWPDDVTPE
                     IEALFKGLR"
BASE COUNT         2694 a         3099 c         2183 g         2867 t
ORIGIN      
        1 cgctgctgcc tcatatgacg ccgctgcccc accggatgca gccgtataca taaccccttt
       61 ttcctgtatg atcccagacc gctgtatctg cccgatttcc ggacacagcc ctgccgcaag
      121 tcccagctgt cccgccaggc acagtcctgc caggatccgt ctgatacagc cccgctgctg
      181 cgttttttcc ttttttatcc tcatgccgcc actttccccg gttccttcct cctatcctat
      241 tcgcaaaatc cttcacatat tcttgcatct gcatacgcct tctgttatga taccatcagt
      301 gcatctggaa acacagcttt cccaaacaaa atgatacaaa cccgacagaa aggattgaat
      361 gcatgataaa acgaagtctg tcctatctta tttccagtgc ggacgccggc cggaccatag
      421 aagaattttt aaggacaaga ggctattccc accgtttgat cgtccatctg agaaatacgc
      481 ctcttggact taccatcaaa ggagaaacag cctatacaac ccatatatta aaggaaaaag
      541 agcttctttg cgtcacgctt atagaggaag aaagttctga acacatcatc ccttccccgc
      601 tccccctttc cattgtctat gaggatgagg atattttggt cataaataag gctccgggcg
      661 tccctatcca tccttcccag ggccattttg aacacactct ggcaaatgcc gtagcctggt
      721 actatcagtc caagggcgaa cccatcatct atcgggccat taaccgactg gaccgtgata
      781 cttcaggact tttaatcatt gcaaagcata tgttaagcgc ctgtatcctt tccgatcaga
      841 tggtaaagcg gcagatccgc cgggaatacc gggcagttgt ctgcggaaac cttccccccg
      901 aagagaccat cacagtcccc atcgcaaggg ttcccggctc taccatcatg cgccagacag
      961 actttgaaaa cggcgaatat gcctgtaccc attttacaaa gctttcctac aacagcaaaa
     1021 atgatctttc ctatatcagc ctgagactgg acacgggaag aacccaccag atacgggttc
     1081 atatggccca tatcggccac cctcttcccg gagattttct ctaccatcct gattaccgct
     1141 ttataaaccg gcagccgctc cactccttcc ggcttgactt tatccatcct cttaccaaag
     1201 aacccatgac ctttttcgca ccgcttccgg atgacatggc ttcccttatt tctgagggtg
     1261 cgcagacgcc tgcatccgtc tgaggccttt gggataatga ttcttaagac agcccccgga
     1321 aagcttgctc cagcctaggc tccagtcttc tgcacacatc aaaacccagc ctttctcatt
     1381 gcccggccag ctctccatcc ggcaggaaag tgcctctcct cttaaataag cggcagtttc
     1441 ttctccgtct gcctccatcc tgtacaccct ttttgcctcc tctggaagca gcgataaggc
     1501 cagtgcatgg gatggttcaa accggttctt tttcacggta cccatatgga gtcccggccg
     1561 tagcaccttc aatcctttca gatccggcat ctcacccggc agaagataaa gctgatctcc
     1621 aaacatcaca tattctgccc ggttcagata agagcttcca tcagcaaggc tttcctttaa
     1681 aaatgcttcc aacagagaaa atacatccct gttccttctg cggtccagat aagcaggata
     1741 cgttcttttt gcttctttac gttcaattcc ctctctgcgc cttattaccg ctataaaatg
     1801 tccctctcct tccagacaat ggggcagaag acggaatgta cgttccagat ggtatattgc
     1861 agcctcttcc tcgtgtgatt tttcctcacc atctccaaac cctgcccatt ccggctttcc
     1921 atgagaaaat ttctcgcatc cttctgtttg ttcaagataa aaatcctcat gacacttcaa
     1981 aaaatttaat accgtacctt cattttcctc cggggcgaag gtacaggtag aatacacgat
     2041 ccgtcccccc ggttttatca tcctggccgc ctgagccaaa atctgttcct gacgggccgc
     2101 acacattttt acatgctctt cgctccactg ttctacggct tcctcatcct ttctgaacat
     2161 accctctccg gagcagggcg cgtctaccac aataccatca aaaaattccg gaaacaccgc
     2221 cgccagacgg gaaggctcct catttaccac tacgccattt gaaacgccca tacgttccat
     2281 attttgggaa aggatctttg ccctggccgg atggatttca ttggaaagaa ggaagccttt
     2341 tccctttaac cgtgatgcta tatgactgga ttttccgccc ggagcagcgc acagatccaa
     2401 aatacgctct ccgggctgag gatccaaaag ctctgtcaca gccatggcgc tgggttcctg
     2461 tatatagtac acaccggctt catggaacgg atgtctgccc ggacgggcct gcgcctcata
     2521 gtagtacccc tccttcaccc agggaatcgg cgtaagagaa gttcccagcc ggttctccat
     2581 taaggcggcc agccccggta aagcttcttt ttcccagcct atcttccgtc cctccaccga
     2641 ttttaagctg ttaaaacgca ggccctgaac ccgttctttt tcatagctgg cttcaaaagc
     2701 ctcatattca tccttaaaca gttctttcat acgcttctta aaggcttcgg gcaatgtgat
     2761 catcattcgt tctccttata ttttattaaa aaaaccgggc tccattttgg ggtccggtct
     2821 taaaaatccg tgtctgtctg ttttacagca caatcttgta atcatccgaa ggaatgccgt
     2881 ttacaacata atatgcagca ttctcctccg ctacgatgta gagttcaaac tctttgatct
     2941 cctcatccgg atggtttttc ttaaagtcct ccatggcggc tgccaaaact tcctttgcgg
     3001 caatatctct gccattatac tggaagtgta cagctgcgct tggctctgct gcttttctgg
     3061 ctgccggctt ctttaccgga gccttcttag ctacggcctt tacttcctca gcagcctctt
     3121 ttactgcctt ctttgcctgt gcagatacgg ctcttgtact ttttcttgtt gccatactat
     3181 cctacctccc tgtagtgtgt atccggccct tgggccatac aaccgggttg tatccggcgc
     3241 aaacgttctt atgtaaatcc tcagatgcaa taagaaaatt atagaatgat ttcctgcttt
     3301 tgtcaatgta cataccgcca caatatgccg cgtttccctt tgatttatca ggatttttcc
     3361 cttttctctg cactttttcc catacatggc cggattcggg agcgaatggt ttcatctgcc
     3421 taccggctgt atgataacgt cattgggcga ggtagttttg ggcgtatctg caccgggatc
     3481 cgcttcattt gaagaaggtt ttcctgtcga ttctgtcact cccggtccgg cagcgccagt
     3541 ttccggattt tgagtgcttt gcgtactgcc cgatccgtat atttcctcag acggctttgg
     3601 cggctgcgtt tcctgcggcg tctcgggcac tgtttctgcg ggctgcaaag gagcagtctg
     3661 aacaggaggc tctgtctcgg ggggcgtttg tgggacggtc tggggaactg tttcaggaac
     3721 agcagtcgga cttgcagatg caggaggctc cgctctccct gatgcactgc ctgccgtgct
     3781 gcgttccgtt ccttccagat tataacagat tacaggcatt cccttatata cattttcata
     3841 cagcgtcttt gcagcttcat aaggcatatt cacacagcca tgagaaccgt ttttcttata
     3901 aatcgtaccg ccaaaggtat tacgccagct ggcatcgtga aagccgatac ctccgttaaa
     3961 cggcatccag aattttacag ggctggcata tccctcgccc tttagcacgg catctctctg
     4021 cttatatgta agactgaaca agcctggagg agttgtatac cccttagact ggtttcctga
     4081 tacgaaatca gattccagga tttttttccc atccttataa aaaaacagat gctgggccgt
     4141 aaggtttact tccgcatagg tagttccata gtcatttccc tcccggctgg cagctgtctg
     4201 cagatacaca ggctccctct ccaccggctc tccggccaga atctcctcct ttagccgcgc
     4261 cttctctcct gctccatcaa tcctccatcc atacactccg gccacttcta cctctggacc
     4321 gtagctggtc ttaaatttcc gtttggtata ggcggtatta tattccgaac caagcgcctt
     4381 tacataggca gccagctgtt cctcatcgat cactggctga tcattttcat cataggaaat
     4441 ccactggctg atcgtttttc cgtccaggat ctctctgcgg cttccaaagg tataggtcac
     4501 agacatgaag gcaaaacgat tcatctgttc caggcgtttc cttaaaaccg gatcgtccga
     4561 gcgcaccgcc ggagcttcat agcagcccgc ctgatccaga tccagctcag gcagaagacc
     4621 gcagatggct tcttctgccg ccttaagagt ccgctcctgg tttaaacgtg ctccttctga
     4681 ctctggcagg atggaatacc cctttccttc ctcatattct gagatcactg cgtctgcagg
     4741 cgcttctgcc gcttcgggat ctgactggga aagtttggaa acggctgctg ccagctgcgt
     4801 ttcatcataa cttataagag cctttatgtc atactcaggc ccccttaaca gataaagcgg
     4861 ccatgaatac ggatcctgct gacgcaggat ttcttccaga tttccatcaa attctgccaa
     4921 aagaccgatc tcatttccaa aaatctgtgc cgtttcaccc tggcgcatag acaggaacag
     4981 acgatatgag gccgcttccg aggcgatcat ctcctttacc tccccgaccg tctttccccc
     5041 cacctccata ccgttgaccg ttgtattagg gaaataggta tccgtatacg ctcctgcctg
     5101 ataaatatat ccgccgatac tgataacaac cgctgctgcc atccctgctc ccactgcctt
     5161 tacagacccc ttttttgtct ttttggccct ggcccgttcc tttctgcggg ctttgaaggc
     5221 ttctctgctg cggctcaccg gccttttctt tttttcttca ctcattcact taaacctttc
     5281 tgactgctcc ttcctcctaa aaacgcttcc cgtctcccaa aacacgtccg ctgaatacaa
     5341 aataaacccc aactgccacc gcactggcaa taacatcggc cgccggctct gcataaaata
     5401 cgttttctat tcccgcaagc gccggaagga tcagcattgc agccagatat acggcttttc
     5461 tgaacatcga ctgactgatt gccagagccg ctgcacccat tcctgtaaat ccgtctacta
     5521 cctcatactg aatagcaaga ggaacaatgg caaggacgct gatgcggatg gcgcgcacac
     5581 tggtttctat aaaaaagcgg tcatttgtaa aaatctcagc aaattgtgcc gacagtgtca
     5641 ttgcaatgac aaacataacc gctgtaaata ccattccaag accaatgatg tgcttctgag
     5701 cttttcggat ccgatccgga cggcctgctc cataattata ccccaaaata gtctgcgtgc
     5761 ctccggtaat tcctcccagc ggcatagtga tcatcagcat aaaactctgc acgatggtat
     5821 tgcaggtcaa aagcacatcg cccatttcgt agcttccata atggcggatc agcatattca
     5881 taataatgat caggccgctg tctgaagccg tgatcaaaaa gggggacagg cccagtacca
     5941 atacacgggc cataagctcc ttttgatatc ctccaaaggt aatccgcacc agaggccgct
     6001 ttcccaaaag gaaaaacagg acataaacac aggaagcaag ctgggaaaga acggtagcga
     6061 tcgcagctcc ctttactccc agatccagca caaagataaa aacgggatct aatataatat
     6121 tgctgacagc ccctaaaagc acggtcatca ttcctacctt tgcaaaaccc tgacagataa
     6181 taaactgatt catgccagtg gaaagaaggg caaaagcagt tcctgacaga taccaggtca
     6241 gatattcatc cgcataagga aaaatagccg aaccggcgcc aaatacggtc agaagctgat
     6301 ttttaaacag atacataacc gtcattaaga cggctgccat gactgcaagg agcacaaagc
     6361 agttagctac cacattttgg gccgcctttt cattcttctg ccccagacgg atgctcatga
     6421 tcggcgctcc tccaatacca acccagctgg caaaggaagt gatcagggtc accactgggc
     6481 cgcagacgcc tacggctgcc agagcctgcc ctccgatttc cggtatgttt cctatgtaca
     6541 tacggtctac gatactatat aaaacactga caaattgagc cagcatggaa ggaaatgcaa
     6601 gcttccatac cagctctctt atgcgatctg tatccagatt attttccagt ttcacgattt
     6661 ttctccttga tataggttat tttcccctga atcgggaaat ttcctctgcc agttcccttg
     6721 cgctgtctgt catgccctta aacgccccaa accggtaaag gctggctata aagaggccga
     6781 tcggcactgc caggatcatt ccggcaagtc cccttatctt aaagccaaga taaagcaaaa
     6841 acagggtcaa aaggggattt aatcccatag tatcccctac cagcttaggc tgcaccagct
     6901 ggcggatcac ctgtgtcagc acatagatca gaatcagccc tgccgcaaag gcatactctc
     6961 cgcccaggac tttgatgatt ccccagggga tcagggcagt tcccgtgcca aatactggaa
     7021 gaaaatcaag cagggcgatc aaaagcgccc ataaaggccc gtagcttacg cccagaatga
     7081 aaaagccggc tgtcaggatc agccatacga cagccataat tttaaactgc gccataaaat
     7141 acccgccgat cagccgtctg gactcttttt tcagatgatt ggaataccgg ccggtccact
     7201 ccggcatatg gcttcttata acagacaaga tccggtcacg gtctgcaata aaaaaataag
     7261 ccgaaagaat gatgaccaca aaatacacca gcgcagccgg gatccctttt gctacagttc
     7321 ctgccgcttc catagtaggc gaggcaatgg actccgccag ctttccaata taggctccca
     7381 gattgtctcc cagggtcatc catgcatccc gcatggtttc aggcatgaaa tcaaagatac
     7441 cttccagccg tcccaggctg ctctgtacat ctccttcaat ccctgcatat aagaccggaa
     7501 gatcctggat aaaggaccag ataaaatgaa gcattcggga tacagcaaga tataaaagcc
     7561 ctatcaccag agccagagct cctgccacga tcacaataga actgtgcctg cgcaccagct
     7621 tcagcctgcg ctctaaaaag cgcaccagcg gattggcgat catggcaatg atccatccca
     7681 cgacaaaagg catgaaaaac cccagaagct tcggccccag aaaacacaga aagaaccatc
     7741 cagaggctgg aatcattata tttaagatca gacgcagaat ataccgccac tggggttctg
     7801 ttttttcccg actgttttta tattcctcca tacggtctca cctgtccttt cctgtacggc
     7861 ccattttatc caaacagctc taatcttcca gcccctttaa cccatatcct tctgccacac
     7921 gcttaaaggc aggaagagaa cgccggatct ggtcaaagga tacgcagtag gaaatcctta
     7981 cataacccgg acaggcgaag gagcttcccg gaaccaccag aatatgttcc tttttgcagg
     8041 cctcacagaa tttcttctca tccggctccg gagattttac aaacatataa aaagcaccct
     8101 ggggctttac acattcaaag ccggcttccc tcagtccccg gtacagaaga ttccggttcc
     8161 tgtcataggc ctctatatct gccgtctcat ccacacaacg cataacaacg cgctgcatca
     8221 gggacggagc attgaccgct cccatgacac ggtttgcgat tacagccgca ttgaatacct
     8281 ctttgctgtc tgtaagctca tccgggatca ccagatatcc gatacgctct ccaggcaggg
     8341 aaagggattt gctgtaagaa tagcagatga ccgtattatg gtaaaatttc gtcacatagg
     8401 gaacctctgc cccgtcatat gccagctctc tgtaaggctc gtcagaaatc aatacgatct
     8461 gtgtgccaaa tttcttttcc ttttctgtca ggatttcccc caaacgcgtc agtacagctt
     8521 ctgaatagat tactcctgtt gggttgttgg gcgtattgat aatgacagcc ttcgtacggt
     8581 ctgtaatccg cctggaaaat tcttccagat ccggctggaa tgtttctgtg tccgccggaa
     8641 ctacctccag tataccatca taatttctca cataattgcc atattctaca aaatagggcg
     8701 caaaggtcac aacctgatcc tgaggattta aaatcgtctt taaaatcaca ttcaggccgc
     8761 tggcagcgcc tacagtcatc aggatatttc cctgatggaa ctttgttcca aaacgccggt
     8821 ttaaggactg tgcgatggcc tcccttacat cttcaaagcc tgcattgctc atatagccgt
     8881 gaaccataac agatggttcc tcattcacaa tcctgcagat cgcctcattg accgcctcag
     8941 gcgcgggcac attgggattt cccaggctga aatcatatac attttctgct ccatatatgg
     9001 ctgccagccg ttttccctcc tcaaacatag cccggattgc ggagttatta tttacaagcg
     9061 gcttcatctt ttctgatatc atactcatgt cctctctttc ccggtaaagt gcagcgttct
     9121 gccctctgac cgctcctttt ctctgtacag gaaaaatgca gaagctgctg ctcatttgat
     9181 tgtatccttg atgggaaaga ttgtcaatac catcaaaaag ccatatcgag acccggcctc
     9241 agacaggcgc aggtttgata tggctttgaa tgaaacaata gttccggcta ccgaagccct
     9301 ttaaaaagag cttctatttc aggcgtgaca tcatccggcc aaaactctgc cgaaactctg
     9361 ctttcatcta aggtcaatgt aacatagaga tcgtaccttg caggctgttc tggctcacgc
     9421 ataccattca tatcagcaag ctccctgtca cgcaatgctc ccataagaag ctcgatctgc
     9481 tcctgttctg taaaaacaag cgttccatct ttctgataga agggattttc cgtctggatc
     9541 tgctttaagg cttctccttc cggaagtccg tcaccgtatt cttccccgtt cacaattgag
     9601 gatacatcca cggaaatccg gcttacatgt tcagccgcaa aataagctcc cggctcaacc
     9661 cccattttct ctaaaacctg tatagtattt gtaaaagagg gataaaccgg ccatctgcaa
     9721 agctgatcca gatggttttc catataccgt tccatctcat ccgcagaagg catttcatta
     9781 taagaatctt tcaatgtttc cgggccaaga agcattttga tcagatctct ctgatattca
     9841 gaaatataac cgtcgttttt tccccaccgt tcctgaatat acaggcacag ctccttttct
     9901 gcaaactgaa gcgctcccac cggatcttct ctcatccggt ccttaagttt ctgcccctta
     9961 agatcggtct gataggctcc aagaagctcc tttatggcat cttcttccat tgtattaaac
    10021 agagaatgat ttccatttcc ctggtaggaa acgtatccca tctcctccgg ctgctgagaa
    10081 aggatcggat aaaggccctt tttatacgtt tcattattat aaatgttttc ataagtttcc
    10141 agcacatctt ctaaatccat gtgatacctg cggctgacct gccggccgtt ctttaaacga
    10201 tagcggataa ggatctggct gaaataccgg tcatacgctt cctcttcctc tttgccggcc
    10261 acaaaaacag aggtcgggcc atctgctcct ccaatgacag atatggctga ggcatcctta
    10321 ctgctataat ggttcacaga catggaaact tcgtttaagc tgatctgcgc ttcccagcgg
    10381 ttcagctttt cttccctgta tcctgccgct tctctggaag cagcctctgc cagagcaata
    10441 acagactcaa aatcggtaag ctccatcttc tcttttataa aatctcccgc actttcatat
    10501 atcagtctcc gggtatctcc ctgccgcatc actcttggca tattggcggc aaacgaataa
    10561 tcaatggata aatccacgct ggcagaagca atttcctcct gagacggcac ataggaatca
    10621 tagccatacc agtcataacg gaagctgcaa aaaaacagca ttcccgttcc caggcacagc
    10681 gccagctgca ttttgtgtga aaaaagcttt ttaaaatcaa aatgatagat gatctcaacc
    10741 acgcagtgca caaggagaat ccctgctgcc gttccaaaca gcccccattt cacctggctt
    10801 tgcagcgtcc agaaaaacca tcctcccgcc attcctccaa gaa
//
LOCUS       DBMDUH010000090        10713 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_3045_length_10713_cov_4.440727, whole genome shotgun sequence.
ACCESSION   DBMDUH010000090 DBMDUH010000000
VERSION     DBMDUH010000090.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 10713)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10713)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10713
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3045_length_10713_cov_4.440727"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            273..1514
                     /locus_tag="ACZPCG_08565"
     CDS             273..1514
                     /locus_tag="ACZPCG_08565"
                     /inference="COORDINATES: protein motif:HMM:NF018485.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1285144.1"
                     /translation="MGTQTKKTNKYMSLAAVTLAFSFTFLSRYIWSPLMADVSGEFGI
                     NATQAGLYMSAFFAGYLVTQIPGGIMADKYQPKYILIACTVLGGAMTALMSAINSYEM
                     GLAIRVITGVSSGCVMAQCSKVVALTFAPKERASAMGVVLASPPFGITLAQSLGAPLN
                     QAFGWRTTFMIVGAVAAVIVVLLAVFVQPVEKKAPEASAPRAAAQTAAAKAPGAGQAG
                     MLEGLVGFFTNKQQLLLGASGFMFMFISVGFATWTNKYVQSIGFSPVQGGMIITCYSI
                     AGIIASCLSGSIAQKLHMSHRNFLMMTLAGMGVMTIIFSMQRSYTGLILVGIIYGAVS
                     YLPSTHYTTSAMMLAGERFTATAASTQNLLFQMASLIMPIIVGRVIDATGSYSFVWYI
                     FVGCAVVAVFFSFLVKNDERP"
     gene            1566..2093
                     /locus_tag="ACZPCG_08570"
     CDS             1566..2093
                     /locus_tag="ACZPCG_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007711027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR1285145.1"
                     /translation="MKELVQKIITLKVNGKDYDITVGTGRNDMPESETLAHTLRDRLG
                     LTGLKIGCDQGACGCCTVIMDGKAVTSCMTLTMDCDGSEITTIEGLADRATGELDGLQ
                     QAFLDNCGYQCGFCIPGVIMTAKALLNENPEPTEEEVREALAGNYCRCGTHYTAVESI
                     MAYVESNKSKKEEQE"
     gene            2090..4384
                     /locus_tag="ACZPCG_08575"
     CDS             2090..4384
                     /locus_tag="ACZPCG_08575"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007711029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR1285146.1"
                     /translation="MKELKYVGKEKVRLLGPQIVTGKAKYTGDFKVPGMQYGKILRSP
                     HPYAKIEAIDVEKAKAYPGVTAVVTWKDVDPNIYITNGFTPPKHHHIMDQYVRFIGDA
                     VALVVAETEDIALEAMKLIDVTYTVLKPVFTIEEALAPDAPQLYPEFPGNIAPHKNNI
                     NFEVGDVEKGFEESDVIVEVDSSIENGQNPLPVEPPVTICWYENDTYNFIASAAAPAY
                     CHQNVASSLNVPYEQVRLTAPAVGGSFGSKLYSGNVQPLVFTAVMAKAAGCPVMFNYS
                     KEEHFAIHQNRMVTKAHLKFGMKKDGLASAVVMNQVADAGVCASTQEFMLAVGTNTLP
                     ILCKTDNKKYDAEVVVTNHMPSGSFRGYGYMESTALVSQAIMEACIKLDIDPVTYLVK
                     NAMKKGDRYHNANAEPHPWQYNKTADWSDLVVKAADAFHWSDRFKGWGVPTWVSADGK
                     KVRGVGVGAAGHSDTGGKPSNANVTLTGLGAVYLSTCMSEFGAGTRDVMLKVVAEELD
                     MPLESIRVSDSDTGMTPPDFGSTGSRSTYCGGIVAKRACEDVKKKLYQLAHEKLGLPE
                     DDLEFKGGYVCRLSNPEEKYSLFPHLMGKVDGVTGCGHFDGVHDSTIFHLQFVEVEVD
                     LEMGTFKIVDHFGGSDAGVIVNPLPLRNQMQSFFAGIDIACMEETVWDPNDYRVLNAS
                     NIDYKTRTFNDVVPHDHIVLESMKNTENDYPFGAVGVGEPLLAPGGPAIRMAIYNACG
                     IKLDSYPFSPAKVLKALKEKEANK"
     gene            4381..6747
                     /locus_tag="ACZPCG_08580"
     CDS             4381..6747
                     /locus_tag="ACZPCG_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007711031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1285147.1"
                     /translation="MKNFTYINPSSVEDASELLKQSGTVAMGGGTDLIGVLKDGLLPE
                     YPEQVVNLKKLPGLSQIEVKEDGLHIGAEATLRQVADSETVKEHWGALSTAATSVATP
                     NIRNTATVAGNLCQDIRCWYYRYPDILGGKIDCARKSGHLCSAMMGENRYHSIFGAAK
                     VCETPCTGNCPAHTDISAYMEMVRAGRYEDAARVILEVNPMPAITSRVCAHFCMEGCN
                     RNTYDESLNVGGIERYLGDMVLEHSEDFMQGPKEENGKKISIVGSGPAGLTAAFYLRQ
                     SGFAVTVYEKQKEPGGCLSYAIPAYRLPKDIVRKFVSVLENMGVTFCCSCNVGTDITL
                     DEIYKISDSVMLDTGTWKRPLIGLAGEELTRFGLEFLIDVNNYILDRPGSDVVVVGGG
                     NVAMDVAITAKRLGAHNVTMVCLEQRDSMPANEEEVTRALEEGVKIVNGWGPKEVLRT
                     DGKVSGIVFKNCPQVLDETGRFNPVYDENNLLTVDADVIMMAIGQKADLDFLEGAYEV
                     ETERGRIKALEGNKTSVEGIFAGGDVTTGPATVIKAIAAGKDTAIAIRKYCQTDALEV
                     EKAAAARLKEKLASFTNECRHNHEAAKASLLSVEDRAMDKEDLSGLDESAVKHEASRC
                     FNCGCLAVNPSDMANMLYAYGAKIRTNMRELDAETFFAGSTRVKDTLKPGEIVLEIVV
                     PMPSEGTTAVYDKYRTRKSIDFAILAVAGTLRLEEGIVKEISLVLGAAAPIPMKLQEA
                     EQYLLGKELTEETAKEAAEIALKKAIPLEMNGYKIEMAKVMIRRFLGF"
     gene            6826..7221
                     /locus_tag="ACZPCG_08585"
     CDS             6826..7221
                     /locus_tag="ACZPCG_08585"
                     /inference="COORDINATES: protein motif:HMM:NF013738.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HIR1285148.1"
                     /translation="MDREKILLYSEKKENLGKPEHFLGAGEVKEPGCGDCLEMFIDTK
                     REVITEFHYTVTDTACPPLKACAARVAECALGKPVMEAYLINAAQLSEFFGGLEKESI
                     HCAQMAEIGLKRTLQDYAVKRAERISKQA"
     gene            7235..8317
                     /locus_tag="ACZPCG_08590"
     CDS             7235..8317
                     /locus_tag="ACZPCG_08590"
                     /inference="COORDINATES: protein motif:HMM:NF045665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043545 - molybdopterin cofactor metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DVU_1551 family NTP transferase"
                     /protein_id="HIR1285149.1"
                     /translation="MKQTGAVIAAAGLSSRMGDFKPLLPFGKECVARHLTQMLARTGV
                     SPVVMVTGYRGEELKQQLKDTDIIFIQNRQYASTEMLDSLKLGFEAIKGQCSEVLVMP
                     VDVPAVTEETVKKIIDADSPIVRTKCAGRRGHPVKLSSVMMEAVLSYQGEQGLKGFLK
                     SCSDLILDVEVADEGSFVDADTKEEYRRLLQLEGSRGQGYPDGEALKWLLETSQMPDH
                     LRRHSEAVSRKALSMAVKLQEKGILLDLNLVRSASLLHDIRKGSADHAAAGAALLKES
                     GYSRAAEIVRQHHWLDRMPSKADETLVVYLADKMIQGDKEVSIDERFEKSLAKCAGNS
                     EALKWHNKKYRQAREAWQVWEESFVS"
     gene            8466..8744
                     /locus_tag="ACZPCG_08595"
     CDS             8466..8744
                     /locus_tag="ACZPCG_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285150.1"
                     /translation="MKRRAVIEYKMHGLWIVPAYCLITGTIGFYADITYSVHTEQENY
                     ILEAAIFAIALLLGRFIFRFFFSKNPVLFSQYNCNISDNHNSIGTGNA"
     gene            9005..9256
                     /locus_tag="ACZPCG_08600"
     CDS             9005..9256
                     /locus_tag="ACZPCG_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285151.1"
                     /translation="MKRNIKKTAVAFFAAAAVFAMGSVSTFAAEPGCGRYYEDADQDG
                     VCDRYGEAGQREAFHRPQREHCGGHHRQEKAGRKHGCRF"
     gene            9288..10202
                     /locus_tag="ACZPCG_08605"
     CDS             9288..10202
                     /locus_tag="ACZPCG_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR1285152.1"
                     /translation="MKIIDSHAHIVQYIAGIGSGGELRSIGGGMARYANGQVVRMIPE
                     QFHTDGVSPEQLLEIMDENGVEKSVLLQGNFYGFQNYYTWEAVKKYPNRFIGAASYDP
                     YSFDRDGIRRFLFEELGFKIEKFEVSTGSGVMAIHPDFLIDGERMEEAFSYGERMGHV
                     MVIDIGKCGSKSWQAEGLKNAVTRHPGVNFVICHLLAPNGKQEEELKRALEGLALPNV
                     WFDLASVPHNCMDASEPWKRAGAYVRLARDLVGADKLLFGTDMPSALKEAPYGDYVRW
                     IREMEGLNDAEREQILYQNAAKVYAGLS"
BASE COUNT         3021 a         2282 c         2771 g         2639 t
ORIGIN      
        1 atgaacaaag aagattgtta tattatctga caaaatcaag aaaaatagtg ctatacaaat
       61 aacattttgt attgtgtgaa aaaaatcgaa aaaagcgtta tttatataaa ttaataacgt
      121 aaattttgaa ttaaattaca aaactatatt gactatatgg ataaaaaagg ttaaaatcaa
      181 cagtatacaa aatgtgtttg tatagcacaa aaaggtcagc tgaattcttt tttttaaaca
      241 caaatgttaa aaaatgacaa aaaggagaag tcatgggaac tcaaacgaaa aaaacaaaca
      301 agtatatgtc attggcggca gtcacactgg cgttttcatt tacctttttg agccggtaca
      361 tctggagccc tctgatggca gacgtatccg gggaatttgg tattaacgcc acacaggcag
      421 gactttatat gtctgctttt tttgcaggat atctggtaac acagattccg ggcggaatta
      481 tggcggacaa atatcagccc aagtatattc tgattgcctg taccgtttta ggcggcgcta
      541 tgacagccct tatgtcagcc ataaacagct atgaaatggg actggcaatc cgtgtgatta
      601 caggtgtgag cagcggctgc gttatggccc agtgttcaaa agtagttgcc cttacatttg
      661 caccaaaaga gagagcttct gctatgggcg tagtgctggc ttccccgcct ttcggcatca
      721 cactggctca gtctttaggc gctcctctca atcaggcctt tggatggcgg actaccttta
      781 tgattgtagg cgcagtggca gccgttatcg tggttttatt agctgtattt gtacagcccg
      841 ttgaaaagaa agcgccggaa gcttccgctc ccagggcagc agcacagacc gcggcagcca
      901 aagctcccgg agccggacag gccggaatgc tggagggact ggttggtttc tttaccaata
      961 aacagcagct tcttctgggc gccagcggat ttatgttcat gtttatcagc gtaggctttg
     1021 ctacctggac caataaatat gtccagagca tcggtttcag ccctgtccag ggcggcatga
     1081 ttattacctg ctacagcatt gcgggcatca ttgcctcctg cctgtccgga agtattgccc
     1141 agaagcttca catgagccac cggaatttcc ttatgatgac ccttgcaggc atgggtgtga
     1201 tgaccattat cttctctatg cagagaagct acaccggact gattctggta ggaattatct
     1261 atggtgcggt ttcctatctg ccttccaccc attacaccac ctctgccatg atgctggcag
     1321 gagagcgctt tacagcaact gccgcctcca cacagaatct gttattccag atggcaagcc
     1381 tgattatgcc tatcattgta ggacgggtaa ttgatgcaac aggaagttat tcctttgtat
     1441 ggtacatctt tgttggatgc gcagtggttg cggtattctt cagctttctg gtaaaaaatg
     1501 acgaacgtcc ataaaaaggt agcttttgat tcaaagatca cttttataga aaggaaaaaa
     1561 gcaatatgaa agaacttgtt caaaaaatca ttaccttgaa ggtaaacgga aaggattacg
     1621 acatcaccgt gggaacgggc agaaatgata tgcccgaaag cgagactctg gctcataccc
     1681 tcagggaccg tctgggcctt acaggcttaa agatcggatg cgatcaggga gcctgcggct
     1741 gctgtaccgt gattatggac ggaaaggcag ttacgtcatg catgacattg actatggatt
     1801 gtgacggctc agagattacc acaattgaag gtctggctga ccgggccaca ggagaactgg
     1861 acggcctgca gcaggcattt ttggacaact gcggatatca gtgcggtttc tgtatccccg
     1921 gtgtaatcat gacagcaaaa gcgcttttaa atgaaaatcc ggaacccaca gaggaagagg
     1981 tcagagaagc actggctgga aattactgcc gctgcggaac ccactatacg gcagtagaat
     2041 ccattatggc ctatgtagaa agcaataaaa gcaaaaagga ggagcaggaa tgaaagaatt
     2101 aaagtatgta ggcaaggaaa aggttcgcct gttaggtcct cagattgtaa cgggaaaagc
     2161 aaagtatacc ggtgatttta aggtgcctgg tatgcagtat ggaaaaattt taagaagccc
     2221 ccatccctat gccaaaatcg aagccattga cgttgaaaag gccaaggctt atcccggagt
     2281 tactgccgtt gtgacctgga aggatgtgga cccgaatatt tacatcacaa acggttttac
     2341 ccctcctaag catcatcata ttatggatca gtatgtccgc ttcatcggag atgcagttgc
     2401 cctggtagtt gccgagacag aggatattgc tctggaagca atgaagctga ttgacgtaac
     2461 ctacacggtg cttaagcctg tattcaccat cgaggaagcg ctggctcccg atgcaccaca
     2521 gctttatcca gaatttccgg gaaatattgc tcctcacaaa aataacataa actttgaggt
     2581 gggagatgtg gaaaagggct ttgaggagtc agatgtaatc gttgaggttg attcttctat
     2641 cgaaaacgga cagaacccgc ttccggtaga gcctcctgtt accatctgct ggtatgaaaa
     2701 cgatacatat aactttatcg catccgctgc cgctcctgca tactgccatc agaatgtggc
     2761 ttcatcctta aatgtgcctt atgagcaggt acgccttacc gctccggcag taggaggaag
     2821 ctttggttcc aagttataca gcggcaatgt gcagcctctt gtatttacag cagtaatggc
     2881 gaaggctgcc ggatgtccgg ttatgtttaa ttacagcaaa gaagagcatt ttgcaatcca
     2941 tcagaaccgt atggttacca aggctcatct taaatttggt atgaagaaag acggtctggc
     3001 aagcgcggtt gttatgaatc aggttgcaga tgcaggcgtc tgcgcttcca ctcaggaatt
     3061 catgctggcg gtgggaacta atactcttcc catcctctgt aaaacagaca ataagaaata
     3121 cgatgcagag gtagttgtta ccaaccatat gccttccggt tccttccgcg gatacggcta
     3181 catggagtct acggctctgg taagtcaggc aattatggag gcatgtataa agctggatat
     3241 tgatcctgta acttatctgg ttaaaaatgc gatgaagaag ggcgaccggt atcacaatgc
     3301 aaatgcagag cctcatccat ggcagtataa caagacggcc gactggagcg atctggttgt
     3361 caaggcagca gatgcattcc attggtctga ccgattcaag ggctggggcg ttcctacctg
     3421 ggtatctgct gacggaaaga aggtaagagg cgtgggagta ggcgctgcag gccattcaga
     3481 taccggcgga aagccatcca atgccaatgt tacccttacc ggactgggcg cagtctatct
     3541 gtcaacctgt atgtctgagt ttggcgcagg taccagagac gttatgctca aggttgtggc
     3601 agaagagctg gatatgccgt tggagtccat ccgtgtgtcc gattctgata caggaatgac
     3661 gccgcctgat ttcggttcca ccggttcccg ttctacctac tgcggaggca tcgtagcaaa
     3721 gcgcgcctgt gaggatgtga aaaagaagct ttatcagctg gctcatgaaa agctcggcct
     3781 tcctgaggat gatctggaat ttaagggcgg atatgtgtgc cgtctgtcca atccggaaga
     3841 aaaatactct ctgttccctc acctgatggg aaaagtagac ggcgttaccg gctgcggcca
     3901 ttttgacggt gttcatgaca gtaccatttt ccatcttcag tttgtagaag tagaggtaga
     3961 tctggaaatg ggtaccttta agattgtaga ccactttggc ggatctgatg caggcgtaat
     4021 tgtaaacccg cttccgctgc gcaatcagat gcagtccttc tttgcgggaa ttgatatcgc
     4081 atgtatggaa gaaaccgtat gggaccccaa tgattaccgg gtattaaacg caagcaatat
     4141 tgattataag acaagaacct ttaatgatgt agttccccat gaccacattg tcctggagtc
     4201 tatgaagaat acagagaatg attatccatt tggcgcagtg ggagtaggag agcccttact
     4261 ggctcccggc ggtcctgcca tccgtatggc aatctacaat gcttgcggca ttaagctgga
     4321 ttcttatcca ttctcacctg caaaagtcct gaaagcgctg aaggaaaagg aggcaaataa
     4381 atgaaaaatt ttacatacat aaatccatct tctgtggagg atgcgtctga gctgctgaaa
     4441 cagtccggca cagttgccat gggcggaggc acggatctga ttggcgtatt aaaggacgga
     4501 ctgcttccgg aatatccgga gcaggtggtt aatttaaaga agcttcccgg cctgtcccag
     4561 attgaagtaa aggaggacgg acttcacatt ggcgcggagg caactctgcg tcaggtagct
     4621 gattctgaaa cagtaaaaga gcactggggc gctttaagca ctgccgctac aagtgttgcc
     4681 acccctaaca tccgcaatac tgctaccgta gccggaaacc tgtgtcagga cattcgctgc
     4741 tggtattacc gctatccgga tattctgggc ggaaagattg actgtgcacg taagagtggc
     4801 catctctgct ccgctatgat gggtgaaaac cgttatcatt ccattttcgg cgcagcaaag
     4861 gtatgtgaga ctccatgtac cggaaactgc cctgctcata cagacatttc cgcatatatg
     4921 gaaatggtac gtgcaggaag atatgaggat gcggcccgcg taatcctgga ggtaaatccg
     4981 atgccggcta ttaccagccg cgtatgcgct cacttctgta tggaaggctg caaccgcaat
     5041 acatatgatg agagcctgaa tgtaggcggc attgagcgtt accttggaga tatggttctg
     5101 gagcattccg aggactttat gcagggaccg aaggaagaaa acggaaagaa aatttccatc
     5161 gtaggttccg gtcctgcggg cctgaccgcc gctttctatc tccgccagag tggctttgca
     5221 gttaccgtat atgagaaaca gaaagaaccg ggcggctgcc tttcctatgc gattccggca
     5281 taccggcttc caaaggatat cgtaagaaaa tttgtttctg ttcttgagaa tatgggtgtc
     5341 acattctgct gcagctgtaa tgtaggcaca gacattactc tggatgaaat ttataaaatc
     5401 agcgacagcg taatgttgga tacaggcaca tggaaacgtc ctctgatcgg gcttgcaggc
     5461 gaagaactca cacggttcgg cctggaattc ctgattgacg taaataatta tattcttgac
     5521 agacccggtt ctgatgttgt tgtagtcggc ggcggaaacg ttgccatgga cgttgccatc
     5581 actgccaaac gtctgggcgc ccacaatgta accatggtct gtcttgagca gagagattcc
     5641 atgccggcaa atgaagaaga ggttacaagg gcactggaag agggcgtaaa gatcgtcaac
     5701 ggctggggac ccaaggaagt tctgagaaca gacggaaaag tcagcggaat cgtattcaag
     5761 aactgtcctc aggttctgga tgaaacagga cgtttcaatc ctgtatatga tgaaaacaat
     5821 ctgcttacag tagatgcaga tgttatcatg atggcaatcg gacagaaggc tgatctggac
     5881 ttcctggaag gcgcttatga ggttgaaact gaacgcggac gcatcaaggc attagaagga
     5941 aacaagacca gcgtggaagg catttttgcc ggaggcgatg taacaaccgg acctgcaacc
     6001 gttatcaagg caattgcggc aggaaaggac actgcgatcg ctatccgcaa atactgccag
     6061 acagacgccc ttgaggttga gaaggcagct gctgcccgcc tgaaagaaaa actggcatcc
     6121 tttacaaatg agtgccgtca taatcacgag gcagcaaagg catccctcct ttctgtagaa
     6181 gacagagcca tggataagga ggatttaagc ggactggatg aaagtgctgt aaagcatgag
     6241 gcatcccgct gcttcaactg cggatgtctg gctgtaaacc caagcgacat ggcaaatatg
     6301 ctgtatgctt acggtgcaaa aatccgtaca aatatgagag agctggacgc agaaaccttc
     6361 tttgcaggct ctacccgcgt aaaggatacc ttaaagccgg gagaaattgt tctggaaatc
     6421 gtagttccaa tgccttctga aggcacaact gctgtttatg ataagtacag aacgagaaaa
     6481 tcaattgatt tcgctattct tgcagtagca ggtacccttc gtctggaaga aggcattgta
     6541 aaggaaatca gccttgtatt gggagcagct gcaccgattc ctatgaaatt acaggaggca
     6601 gagcagtatc tgcttggtaa ggaacttaca gaggaaaccg caaaagaagc agcagagatt
     6661 gcccttaaga aggcaattcc tctggagatg aacggttata agattgaaat ggcaaaggtt
     6721 atgatccgga gattccttgg attctgattt aagccatcca gttggataaa ataaaaagcg
     6781 ggaggacaaa agacctctcc ggcatcaaag aaaggatatt gtaaaatgga cagggagaaa
     6841 attctgttat acagtgaaaa aaaagaaaat ctgggaaagc cggagcactt tctgggagcg
     6901 ggagaggtta aagagccggg atgcggcgac tgtctggaaa tgtttattga tacaaaaagg
     6961 gaagttatta cagaatttca ttatacagtt accgataccg cctgccctcc gttaaaagca
     7021 tgtgccgcaa gagtcgcaga atgtgcactg ggcaaacctg taatggaagc atatctgatc
     7081 aatgccgccc agctgtctga gtttttcggc ggacttgaaa aggagagcat ccactgcgct
     7141 cagatggcgg aaatcggcct taaacggact cttcaggatt acgctgtaaa gcgtgcagaa
     7201 cggatcagca aacaggcata aaagaggtaa acatatgaag caaacaggcg ctgtaattgc
     7261 agcagcgggt ctttcctcca ggatgggaga ttttaaaccc ctgcttccct ttggaaaaga
     7321 atgtgttgcc aggcatctga cgcagatgct tgcgcgaacc ggcgtatccc ctgttgtaat
     7381 ggttacagga taccggggag aagagctaaa acagcagtta aaggatacgg atattatatt
     7441 tatccaaaat cggcagtatg cttctacgga aatgctcgat tcgttaaagc tggggttcga
     7501 agcaattaaa ggacaatgca gcgaggtgct tgtcatgccc gtggacgtgc ctgccgtaac
     7561 cgaagaaact gtaaaaaaga tcatagacgc agacagccct attgtccgga caaaatgtgc
     7621 cggacggcgt ggacatccgg taaaactcag ctcagtaatg atggaagctg tattatcgta
     7681 tcagggagag cagggactga agggcttttt aaaaagctgc tcagatttga ttctggatgt
     7741 agaagtcgct gatgagggca gttttgtaga tgcagataca aaagaggaat accgccgtct
     7801 gttacagttg gagggcagca gaggacaggg atatcctgat ggggaagcct taaaatggct
     7861 cttggaaacc tctcagatgc cggatcacct ccgccgccac agcgaggcag tgagccgtaa
     7921 ggctctttcc atggctgtaa agctgcagga aaaagggata ctcctggatc tgaatctggt
     7981 tcggtctgct tcccttcttc atgacatccg caaggggagc gcagatcatg cagcagcagg
     8041 agcagcgctt ttaaaggaaa gcggctacag ccgggcagcg gagattgtac ggcagcacca
     8101 ctggctggac aggatgccct caaaggcaga cgaaaccctg gttgtatatt tagcagataa
     8161 gatgatccag ggagataaag aagtatccat cgacgaacgc tttgaaaaaa gccttgccaa
     8221 atgtgccgga aacagcgaag cgctgaaatg gcacaataaa aaataccgcc aggccaggga
     8281 ggcatggcag gtatgggaag aaagttttgt ttcataggta agtaaaataa aagtgtcctg
     8341 tccggaggct gaaagccgga tggggcgctt ttttgtttta tatccatgta aaaatgtaaa
     8401 aaaatagtaa gacagcaaaa cacaggtttg tggtatattt aagatatggt atatcctaaa
     8461 ataacatgaa aaggagagct gtaattgaat ataaaatgca cggtctttgg attgtccctg
     8521 cctattgttt gatcacagga acgattggtt tttatgcgga tatcacatac agcgttcata
     8581 cagaacagga aaactacata ctggaagcag caatctttgc aatcgctctt ttgcttggaa
     8641 gatttatttt tcgttttttt ttctcgaaaa atcctgtttt attcagccag tataactgta
     8701 atatttcaga taatcataac agcattggaa ctggtaatgc ctgacctgtt aggcgctctt
     8761 gggatagttc tatttttgta tcctataggc tggtacagaa tcataatacg tctgcttgtc
     8821 agaataggtt ctggtggagt tttatcctat tttttagcat cactatgccc atatctgttt
     8881 attttttttg gaaaacaaac ttcagattga actgcaaaac tgcacagcag acataccggc
     8941 tcccactgga tttagccgtc ggactgtgtt atatttatgg tataaaaaga agggaggaac
     9001 ctctatgaaa cggaacataa aaaagacggc tgtagcgttt tttgcagcgg cagctgtgtt
     9061 tgcaatgggt tcagtaagca cctttgcagc cgagccggga tgcggcaggt attatgagga
     9121 cgcggatcag gacggcgtct gcgaccgtta tggtgaagca ggacagcggg aagccttcca
     9181 cagaccgcaa agggaacatt gcggcggcca tcacagacag gaaaaagccg gcagaaaaca
     9241 tggatgccgt ttttaaataa cagagaaaag gcaggttaaa aggaactatg aagataatcg
     9301 acagccacgc ccatattgtc cagtacatcg cagggatcgg ctccggcgga gagctgcgca
     9361 gcatcggcgg cggaatggcc cgctatgcca acggacaggt agtccggatg ataccggagc
     9421 agtttcatac agacggcgta agcccggaac agcttcttga aatcatggat gagaacggag
     9481 tggaaaaatc cgtgctcctt cagggaaact tctacggttt tcagaattat tatacctggg
     9541 aggctgtaaa aaagtatccg aaccgtttta tcggcgccgc cagctatgat ccctattcct
     9601 ttgaccggga tggaatccgg cgctttctgt ttgaagaact gggatttaag attgaaaagt
     9661 tcgaggtcag caccggctcc ggcgtgatgg caatccatcc tgatttcctc atcgacggag
     9721 aacgcatgga ggaggccttt tcctatgggg agcgtatggg ccatgtgatg gttattgaca
     9781 tcggaaagtg cggcagtaaa agctggcagg cagagggact taaaaacgca gttacccgcc
     9841 atccgggcgt aaactttgtg atctgccacc ttctggctcc caacggaaag caggaggagg
     9901 aactgaaacg ggctctggaa gggctggccc tccccaatgt atggtttgac ctggcaagcg
     9961 taccccataa ttgtatggat gcttccgagc cctggaagag agccggagcc tatgtacggc
    10021 tggcaagaga cctggtgggc gcagataagc tgctgttcgg cacagatatg ccttcagccc
    10081 tgaaagaggc tccgtatggc gactatgtgc gctggattcg ggagatggaa gggctaaatg
    10141 atgcagagcg ggaacagatc ctgtatcaga atgcagcgaa ggtttacgct ggcctttcct
    10201 gatcaaacag accaacatgt tctgcagccc gaaaaccgcc tgatctcttt tcaaaaggag
    10261 acgggcggtt ctttttattt cgggcaggtg tatcgattaa gctcctgact gtgttttcca
    10321 tactcaatca ctttttggga acagcggcag atacaggtca tacagtgggt acatttattt
    10381 ccccaatggg gtttgccgtt tacaagcatt tttgtaaaaa gattacagat tttccccccg
    10441 ggttctttcg ctatcctccg ggcagtatac tcactgtttc ctgttcctgt aaaatataga
    10501 atcatcgtat tttcacttcc tttcattgaa atccattcca ccattataca gaaaaggaag
    10561 gatgataaca gcattaaaaa tatgtatgat agatcatgtt tatctcgccg gtgatcgaca
    10621 tggaaaaaca gaaacgcagg agttgacggc ctatggtgta tgaagatttt ctggcttcaa
    10681 tgagatggtt gtcattcatt taaaaaccgt tta
//
LOCUS       DBMDUH010000091        10322 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_3157_length_10322_cov_3.980041, whole genome shotgun sequence.
ACCESSION   DBMDUH010000091 DBMDUH010000000
VERSION     DBMDUH010000091.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 10322)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10322)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10322
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3157_length_10322_cov_3.980041"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="ACZPCG_08610"
     CDS             <1..397
                     /locus_tag="ACZPCG_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007037857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285153.1"
                     /translation="KNAGETYGYFSGATFANQIGLTTQMPTVLEIVTSRESTKGRIVT
                     VGSQKVRLKRPSIEITSENAGLLQLLDIVSQAKLYSEYSEQKTGEILKRYVRNQRYTR
                     ESLMNSLPAITGTTAKNLIKWGIIYEFTS"
     gene            522..779
                     /locus_tag="ACZPCG_08615"
     CDS             522..779
                     /locus_tag="ACZPCG_08615"
                     /inference="COORDINATES: protein motif:HMM:NF020419.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HIR1285154.1"
                     /translation="MVFKGGTSLTKCYQILERFSEDIDISYAASEGVPGESRKDSSKG
                     LLFPLWMRCSFQSRILKRHGADAVITVIGSAIQASIHRPWN"
     gene            668..1366
                     /locus_tag="ACZPCG_08620"
     CDS             668..1366
                     /locus_tag="ACZPCG_08620"
                     /inference="COORDINATES: protein motif:HMM:NF020419.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HIR1285155.1"
                     /translation="MDALQFPIENLEETRSRRSYNCYRVSYPSLYPPSLELKTQLVIE
                     TYIALLPFPTVQRMTDNYIYRFLKLTDQINLAEEFNLMPFEITTQAIERTLVDKVFAI
                     CDYYMTGKTERHSRHLYDIYKILEYISPDTSLSKLIQEVRKLREPLSICPSAKQDICI
                     NHILTEILESAVYRTDYEIITGKLLFTYVPYETVIEGINKIIKQGYFNISEPTGISPL
                     SMPAAISCLLYRIM"
     gene            1396..1829
                     /locus_tag="ACZPCG_08625"
                     /pseudo
     CDS             1396..1829
                     /locus_tag="ACZPCG_08625"
                     /inference="COORDINATES: protein motif:HMM:NF024771.6"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
     gene            complement(2096..3430)
                     /locus_tag="ACZPCG_08630"
     CDS             complement(2096..3430)
                     /locus_tag="ACZPCG_08630"
                     /inference="COORDINATES: protein motif:HMM:NF024910.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR1285156.1"
                     /translation="MSEDVGYKQVLTRKDLFGIAVGMIIGAGVMTLTGIGIDRTGRSV
                     FISYLIATFFALFFAVPSILSSSIARFKGGNYTVWSMFVGEKWAGAWIIIYFLGEMGI
                     CMYALSFAEYFQALFPSSNPRIVAMAIATLFFVVNFFGINIMAKVENFLTVALVGAIL
                     LFIVCGMPHVDFANYFKNPGFTTHGVVGIFAGGAFLNYAVLGASELISFSGECKNPKK
                     DIPTTIIISTAFVAMMFSLLSIVASGVLPIEEVAGQPLTLVAKEIFNKPLFLFFIVAG
                     ALGATATTLNVTVAYITKPLVQASRDGWFPRSMGELHPKYRTPHKWLFVWYLLTIVPL
                     ALDLSVNQIADLVMFITYLRSIVYAIGYLRMPKMLPDLWAKSMFHMPDWAYRLLMYSC
                     AGVAAFQLISNAMAADIKMIIINIVVLAGAIIFSLVRYHSGKVKMEISYEEA"
     gene            complement(3446..4693)
                     /gene="pepT"
                     /locus_tag="ACZPCG_08635"
     CDS             complement(3446..4693)
                     /gene="pepT"
                     /locus_tag="ACZPCG_08635"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HIR1285157.1"
                     /translation="MKELVERLLRYISIDTQSQHGSQTIPSTEKQKDLGRLLVKELLE
                     MGVENAEMDEFGAVYACIPSNSGEGIPAIGFTAHMDTAPDLPGCNIHPRIIENYDGED
                     IVLNEEKNIIFSTEKFPRLKVYKGKDLIVGDGTTLLGADDKSGVAVIMQMAKTLMTHP
                     EIGHGQVNICFTCDEEIGKSTLNFNLEKFNCDFAYSMDGGELGEFYYDNFNCGIAYVE
                     VKGEVAHTGYAKNRMVNSMHIAMELHSLLNPLEIPANTEGREGFTHLAEIHGDVGTTS
                     MRYLVRDHEMSKFTEKKNALLTIAEFLNKKYGENTVSIRFEDVMYNMRDKILNFPFII
                     DNAVKAMEELGIEPVIIPVRGGVDGAEITFKGLPCPNLFTGGDNCHTIYEYTCIQSME
                     KSYELLVKLCESCLDVRKDADCS"
     gene            complement(4690..5607)
                     /locus_tag="ACZPCG_08640"
     CDS             complement(4690..5607)
                     /locus_tag="ACZPCG_08640"
                     /inference="COORDINATES: protein motif:HMM:NF012607.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR1285158.1"
                     /translation="MFQKTVVVDNTGMNEWAKKRLGELSEEVVFYDDFPTDSDEITER
                     VKDADCLMVSYCTLITRQILEKCPKLKYIGMCCTLYNEKSANVDVAYARERGIVVRGI
                     TDYGDGGVIEFAVSALADLLHGFKGHQWKERPNEFYNQKIGIVGMGNTGFKLARVLKL
                     MEADVSYSDLRCCEEAEKLGICRRTLEELLKENDIICTLLPKNTCLLREKEFELFGNG
                     KIIVNTSIGPTFDVEAMKKWLSRENNYYICDGVGIGDTWEQLHECPNMIYTERCAGSS
                     EQCTLRLSEKSIENVEHFLEEILKKEETR"
     gene            complement(5766..7079)
                     /locus_tag="ACZPCG_08645"
     CDS             complement(5766..7079)
                     /locus_tag="ACZPCG_08645"
                     /inference="COORDINATES: protein motif:HMM:NF013697.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1285159.1"
                     /translation="MKNDNTGVKKQAAAVFIITALCYAGILLYMVQAQKTQFETKGQE
                     VITIWYWDDSIEELFEQYKKDTGSTYRLNYVNVSISDYARQIESAIDLSGELPDICFL
                     QDYYAGNFLPLPIWENLGNEPYGVKYEDYPEGCRSYMTSADGAIVALPYNQDAAGLAY
                     RAEMAKRVFGIDSPGEAMELFADWESLLNKSLEQKEKGHQFMLFNCLGDVGSMLFNQT
                     DEPYTKENKMIKPERFLDYFRYLDKLYDTGLVGNTAQVSMEWSQDYWEETYMMAPWTF
                     WVSQYDTFSVNEKEDWTLISPPNGSFKWGGMVLSIPSSSREKEAAWDFASYLAGSMEG
                     AEANKRVKCRQLTYFGCEHGTEEYRTLEIEGFGNQDIGNYVSSELMSHRKERPLSSYD
                     LELDEVFHLTIDAIIHEPELTPEELYAYFLRMLKKAVPKLQVPPI"
     gene            complement(7069..8847)
                     /locus_tag="ACZPCG_08650"
     CDS             complement(7069..8847)
                     /locus_tag="ACZPCG_08650"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing sensor histidine kinase"
                     /protein_id="HIR1285160.1"
                     /translation="MRQSIWKKIVAAAAAAIVAMTLIGMGISCSYAYINQYRQQRHRD
                     EAAMALCANQCQTLLNDVRMLAHSVAENELVQRFYSQDTHEYFETIEAIQEIKETIHL
                     RVHIKNAVLIQGNQVYWSYSPFEPYFEERLSEEWYQEIKDEREAFSRVHTCGYPENSR
                     YLTYLLSIPDKGTPDKELGKLLLNINVQYFSDIIGETAGLSSGYFVSDLRDGTVILGQ
                     GNQETNIRLAEKMAASVQSEKKYRGEMEIGSGDKGYCLWSAFPDAGIGVGMYTSSGLL
                     FQYSEQMRYFFVVYSVSTAAAILLLTFLLIRKFLQPISQISAAMLDFSGGKRKILEIK
                     TGDELEMLSCQFNKMAERIEEFIKRRIQDERINKKLKFDMLMSKIHPHFLYNTLNSVI
                     YLARRDHNQDIEKMVQSLILILQDGMAAYSGKSCAMLKEELEVVSAYCVIQNYRYKDK
                     VSVSIEVPDELTTAYVPKNILQPLVENSIYHGIAPMEENGSIEILAEQREDMLDLWVI
                     DTGIGMDEEELKGLEQNGKGHSGVHSIGIKSIRERLEYLYPEHYSFEIYSEKGKGTTI
                     LISVPYQKDLSLLREAEEDEGEIHEE"
     gene            complement(8849..>10322)
                     /locus_tag="ACZPCG_08655"
     CDS             complement(8849..>10322)
                     /locus_tag="ACZPCG_08655"
                     /inference="COORDINATES: protein motif:HMM:NF012301.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1285161.1"
                     /translation="YGFTIAQTFTNGVAALRGIREIRPDFIVTDIRMPIMDGLQFVEK
                     VRTLLPETRILLLTAYSDFDYAKKAIDLGVFNYLLKNELNKETLEEQLERVFLDLMRN
                     KDIKAAMVQKAFRKFLYAPERRELQEELFHELAEHYGQAVGFFYVIPMSASEGEHFAG
                     SVCTALSDCHVMPAENGNEIYTVCEAWERRYVIVLLCMKRMPASRLETAGIIRMAAEK
                     LSLCLQKRVSARVLYRQEGGIVSDMEQAAELLKSIQAAVPYGIFAGDKREVEISELVG
                     RGQKDVWESKTEERLYHWFASKEPYEIKNGLSDIYKEAEKNGSVKQASIIAGAMENAV
                     KEYVKRGICSPEDSKEIRQLTESFCDLRELFGGYERFLERIESEDKEKSYSTRVIKVI
                     RFIRQNYEKDISVGDAAQLLGLNGEYLNKLFKKETGESFSRYLTQIRMEKAKELLESG
                     QFNVSEVSARTGYKSSQYFSITFRRYMGYSPSEAEAHKGD"
BASE COUNT         2848 a         2611 c         1855 g         3008 t
ORIGIN      
        1 aaaaaatgcc ggagaaacct atggatattt ttctggcgcc acttttgcaa accagattgg
       61 tctgacaacg caaatgccca ctgtactgga aattgtaaca tccagagaat ccaccaaggg
      121 acgtatcgtt acagtaggtt cgcagaaggt tcgcttgaaa cgtccatcta tcgaaattac
      181 ttctgagaat gcaggattgc tacagctttt ggatattgtt tctcaggcta aattatattc
      241 ggaatattca gaacagaaaa cgggagaaat attgaaacgt tatgtaagaa atcaacgtta
      301 taccagagaa agtcttatga actcactacc agccattaca ggtacaacag caaaaaattt
      361 gattaaatgg ggaattattt atgaatttac atcttgatcg tgaggctttt acggaacttg
      421 taactgcggc ctcacatgaa ttacacatac cttcaggcat tatagaaaaa gattactatg
      481 taacacttgc attgcgggaa ttatctgccc gtatcaatgg aatggtattt aaaggcggca
      541 catctttgac caaatgctac caaattttag aacgtttttc cgaagatatt gatatttctt
      601 atgccgcctc tgagggagtc cccggggaaa gcaggaaaga cagttcaaaa ggtctattgt
      661 ttcctctatg gatgcgctgc agtttccaat cgagaatctt gaagagacac ggagccgacg
      721 cagttataac tgttatcggg tcagctatcc aagcctctat ccaccgtccc tggaactgaa
      781 aacacagctg gtcattgaaa catatatcgc actgcttcct tttccaacgg ttcagaggat
      841 gaccgataac tatatttatc gttttctaaa acttaccgac cagataaatc tggcagaaga
      901 atttaatctt atgccatttg aaataacaac gcaggcaatc gaacggacac tggttgataa
      961 agtatttgcc atttgcgatt attatatgac agggaaaaca gaacggcatt cgcgccatct
     1021 gtatgatata tataagatat tggagtatat atcgccagat acatcattgt ccaaactgat
     1081 acaggaagtc cgaaaacttc gggagccact gtctatctgt ccttcagcaa agcaagatat
     1141 atgtatcaat cacattttaa cagaaattct tgaaagtgct gtatatcgaa cagattatga
     1201 aatcattaca ggaaaattac tttttacata tgttccatat gaaaccgtta ttgaaggaat
     1261 aaacaaaata attaaacagg gctattttaa tatatcggag cctacaggga taagccccct
     1321 gtctatgcca gcagcaattt cctgcctact gtacaggatt atgtagacga agccctttat
     1381 tacaagagca aaggcatcaa aggctataag gctcatcccg caagacctgt agaatttgat
     1441 atgaaggttc acgctgcagt ccgggaggcc gtggaagatg attttatcct gatgacagac
     1501 cctgtagacg actacacgct ggatgaagca atccgggtag gacggcagct ggaaaactga
     1561 actataagtg gtttgaagaa cccttccgtg attttgagct ttataagtat tccgagctgt
     1621 gtagggctct ggatattcct attgccgcca cagagaccac cggaggctgc cactggggag
     1681 tcgctcaggt aatcgcccag agggccgctg atatcatccg ggcagacgta agctggaagg
     1741 acggcatcac aggcgcgtta aaaattgccc atttatctga ggcctttgga cttaacgatc
     1801 ctaaaagctc ccaaaaagat ctatcataaa tataaaataa accttcaaac tgttataaga
     1861 taaaatcaat cactcagttt gaaggtttat tttaaaatgg aaaattttct gacataaata
     1921 caaactcata gtgaaataca cctttcaaac tcaatggatg cacatcattt tttaccgact
     1981 tatgacttaa cagaaaagca gcccggcgcc gtgatgccga agctgcttcc cttactaaat
     2041 gatttttccg ttatcctttt ttaatcctgt tatatataat ttccttccgt ccctcttatg
     2101 cctcttcata agagatttcc atcttcacct tgccgctgtg ataacgtacc agtgagaaga
     2161 tgattgcgcc tgccagcaca acaatattga taataatcat cttaatatct gctgccattg
     2221 cattggagat cagctggaat gctgcaacgc ctgcacagga atacattaac agtctgtatg
     2281 cccagtccgg catatggaac atggattttg cccacagatc cggaagcatc ttcggcattc
     2341 tcagatagcc aatagcatat acgatagaac gcagatatgt aataaacatt accaggtcag
     2401 caatctggtt tacactcaga tccagagcca gcggcacgat tgtcagcaga taccatacaa
     2461 acagccattt atggggagta cggtactttg gatgcagctc gcccatagac cttgggaacc
     2521 atccgtctct tgaagcctgt accagaggct ttgtgatata tgcaaccgta acgtttaacg
     2581 tagtagcggt agctcccagc gcgcctgcca caataaagaa caggaacagt ggtttgttaa
     2641 aaatctcttt tgctaccaga gtaagaggct gacctgctac ctcctcaatg ggaagtacgc
     2701 cggaagctac aatggaaagc agtgaaaaca tcatagctac aaatgcggta gaaatgataa
     2761 tggttgtagg aatatccttc ttgggattct tacattcacc ggagaatgaa attaattcag
     2821 aagcaccaag aactgcatag tttaaaaatg ctccgcctgc aaagattcct actactccgt
     2881 gagttgtaaa gcccggattt ttaaagtaat ttgcaaaatc cacatgaggc ataccgcaga
     2941 cgataaataa aaggattgct cctaccaggg caacggtcag gaaattttcc acctttgcca
     3001 taatgttgat tccaaaaaag tttacaacaa aaaacagtgt cgcaatcgcc atggcgacga
     3061 ttctgggatt agatgagggg aacagtgcct ggaaatactc tgcaaaggac agagcataca
     3121 tacagattcc catctcgccc aggaagtaga tgataatcca tgcgccagcc catttttctc
     3181 ctacaaacat ggaccatacc gtataatttc cgcctttaaa acgggcgata ctggaactta
     3241 aaatactggg aaccgcgaaa aacagcgcaa aaaaggtagc gatcagataa gagataaata
     3301 cggagcgtcc cgttctatca atcccgatac cggttaatgt cataacgccg gctccgataa
     3361 tcatgcctac tgcaattcca aataagtctt ttcgggttaa gacctgctta tatcctacat
     3421 cttcactcat gttgcctttt cctccttatg aacaatctgc atcttttctt acatccagac
     3481 aactctcaca cagcttcacc agcagctcat aggatttctc catggactga atacaggtat
     3541 attcataaat agtgtggcag ttatctcctc ctgtaaacag gttggggcat ggaagccctt
     3601 taaatgtaat ttctgctccg tcaactccgc cgcgcacagg gatgattacc ggctcaattc
     3661 ccagctcctc cattgccttc accgcattgt caataataaa cgggaaattc aggatcttat
     3721 ccctcatatt gtacataacg tcctcaaagc ggatgctgac cgtattttca ccgtatttct
     3781 tattaaggaa ctctgcaatt gtcagcaggg catttttctt ttccgtaaat ttactcatct
     3841 catgatcccg gaccagatac cgcatggagg tagttcccac atctccgtga atctctgcca
     3901 gatgagtaaa gccttctctt ccttctgtat tggccggtat ctccaatgga ttcagcaggc
     3961 tgtgcagctc catggcaata tgcatggaat ttaccattct gtttttcgca tatcccgtat
     4021 gggctacctc tccttttacc tctacatagg caattccgca gttgaagtta tcatagtaga
     4081 actctcccag ctctcctccg tccatagaat aggcaaaatc gcagttgaat ttttccaggt
     4141 taaaattcag cgtgcttttt ccaatctctt catcgcaggt aaagcagata tttacctgtc
     4201 catggccgat ttccggatgt gtcatcagcg tctttgccat ctgcatgatt acggctacac
     4261 cgctcttatc atctgctcct aaaagggtgg ttccgtcgcc gacgatcaga tccttcccct
     4321 tataaacctt caatcttgga aatttttctg ttgagaaaat aatatttttt tcctcattca
     4381 gaacaatatc ctctccgtca tagttttcta tgattcttgg atgaatattg cagcccggaa
     4441 gatccggcgc agtatccata tgggccgtaa agccaatggc cggtattccc tctccgctgt
     4501 tggagggaat gcatgcataa actgctccaa attcatccat ttccgcattt tccacaccca
     4561 tttccagaag ctcctttacc agaagccttc caagatcctt ctgtttttct gtactgggaa
     4621 tggtctggct tccatgctgc gactgggtat cgatgcttat atacctgagc agccgttcta
     4681 ctaactcctt catcgtgttt cctccttttt cagtatttct tccagaaaat gttctacatt
     4741 ttcaatgctc ttttcagaca gacgcagggt acactgctca gagcttcctg cacagcgttc
     4801 tgtataaatc atgttgggac attcatgaag ctgttcccat gtatccccga ttccaacgcc
     4861 gtcgcaaata taataattat tttcccggga cagccacttc ttcattgctt ccacatcaaa
     4921 tgtaggtcct atggacgtat tgacaatgat ctttccattt ccaaataatt caaactcttt
     4981 ttcccttaaa agacaggtat ttttagggag gagggtacag ataatgtcat tttcctttaa
     5041 cagttcttct aaggtccggc ggcaaattcc cagcttttct gcctcctcac agcaccttaa
     5101 atcagaatag gacacatctg cttccatcag cttcaaaact cttgccagct taaagcctgt
     5161 atttcccatt cccacaattc ctatcttctg attataaaat tcattgggcc gctccttcca
     5221 ctgatgtccc ttaaatccat ggagcaggtc tgccagtgca cttactgcaa actcaatgac
     5281 gcctccgtcg ccgtaatctg taattccccg gacaacgatg cccctttctc tggcataggc
     5341 tacatctaca tttgcgctct tctcattata aagggtacag cacatcccaa tatatttcag
     5401 cttagggcat ttttccagaa tctgtcttgt aatcagggta caataggata ccatcagaca
     5461 gtctgcatcc ttcaccctct ctgtaatttc atccgaatcc gtggggaaat catcgtaaaa
     5521 taccacttcc tctgacagct ctccaaggcg tttcttcgcc cattcgttca ttcccgtgtt
     5581 gtctactacc actgtttttt gaaacatccc ttctgcttcc ctcccgtctg atttctggca
     5641 tttatttatg caatttgttt tttatttaat gtttatcctc tttgttttta taaaataatt
     5701 ataaaaaaaa tccactgatc catatacatg aaaaagtgga ttttttgttc aaaaacagga
     5761 tatttttata tcggcggcac ctgtaatttt ggcactgcct ttttcagcat acggagaaaa
     5821 taagcgtaca gctcctccgg cgtcagctcc ggctcatgga taatcgcatc aatagtcaaa
     5881 tgaaatacct catccagctc cagatcatag gacgaaagcg ggcgctcctt tctgtgactc
     5941 ataagctcac tgctcacata atttcctata tcctgatttc caaagccttc aatctccaga
     6001 gttctgtatt cctctgttcc gtgctcacag ccaaaatacg tcagctggcg gcattttacc
     6061 cgcttattcg cctctgcccc ctccatactt ccagccagat aactggcaaa atcccatgca
     6121 gcctcctttt ccctgctgga ggacggaatt gacagcacca ttcctcccca cttaaaactt
     6181 ccatttggag ggctgatcag cgtccagtcc tccttttcat taacagaaaa tgtatcatac
     6241 tgggaaaccc agaatgtcca cggagccatc atatatgttt cttcccaata atcctgagac
     6301 cattccatgg atacctgagc cgtatttccc accagacctg tatcatagag cttgtccaaa
     6361 tagcggaagt agtccaggaa acgctccggt tttatcatct tattctcctt cgtatagggt
     6421 tcgtctgtct gattaaacag catagacccc acatctccca gacaattgaa cagcataaac
     6481 tgatgcccct tttccttttg ctccaggctt ttattaagaa ggctctccca gtctgcaaac
     6541 aactccatgg cttcccccgg cgaatcaatt ccaaaaaccc gttttgccat ttcagcccga
     6601 tacgccagcc ccgccgcgtc ctgattatag ggaagagcca cgatagctcc gtcagccgag
     6661 gtcatatagc ttctgcaccc ttccgggtaa tcctcatatt ttacaccata aggctcattc
     6721 cccagatttt cccagattgg aaggggaagg aaattacccg catagtaatc ctgaagaaag
     6781 cagatatccg gaagctctcc cgacaaatca atggcgcttt caatctgtct ggcataatcg
     6841 gaaatagata catttacata attaagccgg taggtgctcc ccgtatcctt cttatactgt
     6901 tcaaacagct cctctatgga atcatcccag taccagatgg taataacctc ctgccccttt
     6961 gtctcaaact gagttttctg ggcctgaacc atatataata aaattcccgc atagcacaga
     7021 gcagtgatga taaatactgc cgctgcctgc ttttttacgc ctgtgttatc attcttcatg
     7081 aatctcccct tcatcttcct ctgcttccct cagcagtgac agatccttct ggtagggaac
     7141 cgatattaaa atcgttgtcc ccttcccttt ttcactgtaa atttcaaagc tgtaatgctc
     7201 cggatacagg tattccagac gttccctgat gcttttaatt ccaatagaat gcactcctga
     7261 gtgacctttt ccattttgtt ccagcccttt cagctcttct tcatccatac caatacccgt
     7321 atcaattacc caaagatcca gcatatcctc cctctgctct gccagaatct caatagaacc
     7381 attttcttcc atgggcgcaa ttccatggta aatggaattt tccaccagag gctgaaggat
     7441 atttttcggt acataagctg ttgtaagttc atccggaacc tctatactta cagaaacctt
     7501 atccttataa cggtaattct gaattacgca gtaagccgac accacctcca gctcctcctt
     7561 cagcatagcg cagctttttc ccgaatacgc agccattcca tcctgaagta tcagaatcag
     7621 agactgtacc attttctcaa tatcctgatt atggtctctt ctggcaaggt aaataactga
     7681 atttaaggta ttataaagaa aatgcggatg gattttcgac ataagcatat caaatttcag
     7741 ctttttatta atccgttcgt cctgtatcct tcgtttgata aactcttcga tccgctctgc
     7801 catcttatta aattgacagc tgagcatctc cagctcgtcc cctgttttta tttccagaat
     7861 tttccgtttt cctccggaaa aatccagcat tgccgccgat atctgggaaa taggctgtaa
     7921 aaacttccgt ataagcaaaa atgtcaaaag aaggatcgcc gctgcagtgg acacggaata
     7981 cacaacaaaa aaataccgca tctgttctga atactgaaac agaagcccgg atgatgtata
     8041 catacccact ccaatacccg catccggaaa agcgctccac agacaatacc ccttatctcc
     8101 tgaaccgatt tccatttctc ctctgtactt tttttcactt tgaacagatg ctgccatttt
     8161 ttctgctaga cgaatattcg tttcctgatt tccctgccct aaaataaccg ttccatctct
     8221 taagtctgat acaaaatatc cgctgcttaa gcctgctgtc tctcctataa tatctgaaaa
     8281 atactgcaca tttatattta acagaagctt tcccagctcc ttatccggtg tacctttatc
     8341 cggaatactc aacagatagg tcagataccg ggaattttcc ggatatccgc aggtatgcac
     8401 ccggctgaag gcctctctct catcctttat ctcctgatac cattcttctg aaaggcgctc
     8461 ttcaaaatag ggctcaaagg gactgtagga ccagtacacc tgattcccct ggatcagaac
     8521 tgcgttttta atatgtactc ttaagtgtat ggtttcctta atttcctgaa tcgcctcgat
     8581 cgtttcaaaa tattcatggg tatcctgact ataaaaccgc tgtaccagct cattttcggc
     8641 aacactgtga gccagcatac gcacatcatt taacagtgtc tgacactgat tcgcgcacaa
     8701 agccatagct gcctcatccc tgtgtctctg ctgacgatac tgattgatat aagcatacga
     8761 acaggaaatc cccataccaa tcagcgtcat tgccacaatg gctgctgcgg cagccgcaac
     8821 tatttttttc catatggact gtctcaattt aatctccttt gtgtgcctct gcctcagatg
     8881 gagaatatcc catatagcgg cggaaggtaa tgctgaaata ctgactgctc ttatagcctg
     8941 tacgggcaga aacttcactt acattaaact gtccgctttc caaaagctcc tttgcctttt
     9001 ccatacggat ctgagtcaga taacgtgaaa agctttcacc tgtttctttc ttaaaaagct
     9061 tattcaggta ttctccatta agtcccaaaa gctgggccgc atcaccaact gatatatctt
     9121 tttcgtagtt ttggcggata aaccggatta cctttataac acgtgtgctg tagctctttt
     9181 ccttatcttc tgattctatc ctttctaaaa aacgttcata tccgccaaaa agctcccgaa
     9241 gatcacaaaa gctttccgtc agctgccgta tttcctttga atcttctgga gaacagattc
     9301 ccctcttcac atactccttt actgcatttt ccatcgcgcc tgcaattatg gatgcctgtt
     9361 tgacagaacc atttttttct gcctccttat aaatatcgga aagtccgttt tttatctcat
     9421 atggttcttt tgaagcaaac cagtggtaca ggcgctcttc tgttttgctc tcccacacat
     9481 ccttttgccc ccttcccaca agctctgaaa tttccacctc ccttttatct cctgcaaaga
     9541 ttccataggg aactgctgcc tgaattgatt ttaaaagctc cgctgcctgc tccatatcgg
     9601 aaacaattcc tccctcctgg cggtacagca ctctggcaga aacccgtttt tgaaggcata
     9661 atgacagttt ttcagcggcc atccgaataa tccctgctgt ttccagccgg cttgcaggca
     9721 tccgcttcat acataacaaa acaattacat acctccgctc ccatgcttcg caaaccgtat
     9781 atatttcatt tccattctct gccggcataa catggcagtc tgaaagagcc gtacagacgc
     9841 ttcctgcaaa atgttccccc tctgaagcgg acattggaat tacataaaaa aatccaaccg
     9901 cctgtccgta atgctctgcc agttcatgaa aaagctcctc ctgaagctct ctgcgctccg
     9961 gtgcatacaa aaatttgcga aatgccttct gcaccatagc tgcctttata tccttattcc
    10021 gcatcagatc cagaaacacc cgttccagct gttcctccag agtttcctta ttcagttcat
    10081 ttttcaaaag ataattaaat actcccagat caattgcctt tttggcataa tcaaaatcag
    10141 aatatgcagt caaaagcaga atccttgtct ccggaagcaa tgttcttacc ttctcaacaa
    10201 actgcagccc atccataata ggcatacgaa tatctgttac aataaaatcc ggtcttatct
    10261 ctctgattcc ccgcaatgct gccaccccgt tcgtaaaggt ctgagcaata gtaaatccgt
    10321 at
//
LOCUS       DBMDUH010000092        10262 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_3174_length_10262_cov_5.233934, whole genome shotgun sequence.
ACCESSION   DBMDUH010000092 DBMDUH010000000
VERSION     DBMDUH010000092.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 10262)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10262)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10262
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3174_length_10262_cov_5.233934"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="ACZPCG_08660"
     CDS             <1..284
                     /locus_tag="ACZPCG_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008725458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR1285162.1"
                     /translation="TTLTALPKNFGYNMSIMILVYVVLGGIGNIRGSVIAAVVLYMLP
                     ELLRGLSNYRMLIYAVVLILAMLFNSAPQFVVLRERIAQNFRKKEKEAA"
     gene            284..1042
                     /locus_tag="ACZPCG_08665"
     CDS             284..1042
                     /locus_tag="ACZPCG_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1285163.1"
                     /translation="MALLEVKNLSISFGGLKAVDGFHIDIEKGQLYGLIGPNGAGKTT
                     VFNLLTGVYKPNAGSIVLDGKNITGKSTIQINQEGIARTFQNIRLFADMSVLDNVKTG
                     LHNHHRYSTLEGIFRLPRYHKTEKEMDEKAMSLLEVFGLAGECDFKASNLPYGKQRKL
                     EIARALATDPKLLLLDEPAAGMNPNETAELMDTIRFVRDNFDMTILLIEHDMKLVSGI
                     CEKLTVLNFGQVLREGSTSQVLHDPEVVKAYLGE"
     gene            1057..1767
                     /locus_tag="ACZPCG_08670"
     CDS             1057..1767
                     /locus_tag="ACZPCG_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1285164.1"
                     /translation="MAMLEVRDLEVYYGVIQALKGISFDVDQGEIVALIGANGAGKTT
                     TLHTVTGLLSAKAGKIVYEGTDITRVPGYKLVSMGIAHVPEGRRVFATLTVLQNLKMG
                     AYTRKDKNEMEETLKMIYKRFPRLEERKNQLAGTLSGGEQQMLAMGRALMSHPRLIVL
                     DEPSMGLSPIYVNEIFDIIQKINEDGTTVLLVEQNAKKALSIADKAYVLETGTIALSG
                     DAKELMNNDQVKKAYLSE"
     gene            1821..3302
                     /gene="spoIVA"
                     /locus_tag="ACZPCG_08675"
     CDS             1821..3302
                     /gene="spoIVA"
                     /locus_tag="ACZPCG_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008725461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HIR1285165.1"
                     /translation="MDNFNVYRDIEARTGGEIYIGVVGPVRTGKSTFIKRFMELLVLP
                     AMEDEHLRAISRDELPQSAAGKTIMTTEPKFIPKEAAKISLDDGIEARVRLIDCVGFM
                     VEGAAGHVENEEERLVKTPWFDHEIPFTQAAELGTRKVIGDHSTIGIVVTTDGTIGDL
                     KRPGYIAAERQTVEELKKLGKPFVILLNSVKPYSEETARLAKELEETYGVSVLPVNCE
                     QLKKEDIFHILECVLKEFPVTEMDFHIPKWLEILPASHWLKARVIQTAKEILQKVEHM
                     KDVALRIREMKDESGAIKSFSVRKMEMADGSVDLAVEMDDSYYYQILSDYVGLPIGGE
                     YELMQTLSGLARMQKEYEKVKNALAQARIKGYGVMMPEREEILLDEPQVIRHGNKYGV
                     KMKAQAPSINLIKAHIETEIAPIVGSEQQARDLIAYIKSNAGESEEGVWDANIFGKSI
                     EQIVEDGIQAKISQMTEDCQMKLQDTLQKIINDSNGGMICIII"
     gene            3402..5009
                     /locus_tag="ACZPCG_08680"
     CDS             3402..5009
                     /locus_tag="ACZPCG_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HIR1285166.1"
                     /translation="MHMKLTFIGADHEVTGSCHFLEAGGSKVLIDCGMEQGNNPYVNA
                     ELPVSYSEIDYVFLTHAHIDHAGLLPWIYARGFKGRVITTYATMDLCQIMLRDSAHIQ
                     EMEAEWKNRKAKRAGRGEVEALYTVEDAEGVLSHFEGVAYGQVFKLNDNLTLRFTDVG
                     HLLGSASIEIWAKEGDEERKIVFSGDIGNKSKPLIRDPQYISEADYVVMESTYGNRYH
                     KKTTTYVESLTRIIQETLDRSGNVVIPAFAVGRTQELLYMIRHIKEHNLVHGHDGFLV
                     YVDSPLAVEATQVFTENLLDCYDDETKALVERGINPIDFKGLKLSVTSDESKNINFDM
                     TPKVIISAAGMCDAGRIRHHLKHNLWRPECSIVFAGYQAEGALGRVLQDGAPAVKIFG
                     EEIDVRAHIETIDGISGHADRDGLLAWISAFEKQPSHVFVVHGNEESCTAFTEALQNQ
                     LRLTADAPYSGSQFDLIKNCWICQTEPVLVEKETEARRKATGVYTRLVDAGSRLLDVI
                     SHNQGGANRDLARFTEQILALCEKWDR"
     gene            5053..5532
                     /gene="rnhA"
                     /locus_tag="ACZPCG_08685"
     CDS             5053..5532
                     /gene="rnhA"
                     /locus_tag="ACZPCG_08685"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HIR1285167.1"
                     /translation="MTEVQIYTDGSARGNPDGPGGYGVVMHYTDRTGKRYEKTMSGGY
                     VRTTNNRMELMAAIAGLEALNRPCAVSLYSDSKYLTDAFNKHWIEGWVAKNWDRGKSG
                     PVKNIDLWKRLLKAKEPHQVTFIWVKGHAGHPENERCDQLATTAADGTDLMIDEGLQ"
     gene            5661..6422
                     /locus_tag="ACZPCG_08690"
     CDS             5661..6422
                     /locus_tag="ACZPCG_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HIR1285168.1"
                     /translation="MKDKRIWLVILGILVIGILVTGYTRSVVKKQADSAAFEGEISTE
                     ASLGPFKEQTEEEAQKEESGAVQEQAQADTAVPETEQKEEEAGEPAAISEPVPAMARS
                     AVPESGRAATDSQADSPCEGGLYRQRLNDLDAQIQKMQEQERDPNVYSIKTSAETEVK
                     MWDRELNAVYNALLGILPQEEAEELAKEQKEWLINRETAAGQSGKAEGVGSISYAASL
                     VDLTRNRAYELADRYEKEACGPQDAENESDTAVLP"
     gene            6557..7369
                     /locus_tag="ACZPCG_08695"
     CDS             6557..7369
                     /locus_tag="ACZPCG_08695"
                     /inference="COORDINATES: protein motif:HMM:NF012669.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HIR1285169.1"
                     /translation="MNKAEERYQEIIEILKENPIVTVADFAERLSVSMETIRKDLNIL
                     SEQGKIVRVHGGAALAGNTEMASPFQFREGVRRREKKKLGKEAVKLIQKGDTLIIESS
                     TTTAEFVKELVKVPELLKTLTIVTNSIHILTLVEMGKLCKRVFLLGGWINEREGATKG
                     QFMAEQLKSFHVDKCILSGAALGKNLILSSYYESDMHFQKLAMESAGETILLLEAGKY
                     PEAAVFSVTAATNFDYMVSNVPFKKEDKERFAAAGLHLIYVPTGSEEATDND"
     gene            7503..8465
                     /locus_tag="ACZPCG_08700"
     CDS             7503..8465
                     /locus_tag="ACZPCG_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HIR1285170.1"
                     /translation="MIPNPFGFSYSDDIDISEMEQSIARLKRPQGRIDVVLDTDTFNE
                     VDDQFALAYLLQSEDRLNLKAIYAAPFDNHHSNGPKDGMERSYEEIFRVLAMLKKSQY
                     EECTYKGAEQFLDSEETPAVSAAAEHLCSLAMEYTKEDPLYVIAIAACTNIASALLMK
                     PEIKDRIFVVWLGGLSLDWHDNFSFNAKQDVAAARVLLGSGVPLVLLPGRGVVDHFST
                     TGPELEYWLRGKNEFCDYIIDKTRHEAEIVSGETCWSRPISDVTAVAWLLDTSFMLDR
                     LEPSPIMEYDHHYAKDKRRHFIRYVYSINRDRLMKDLFDKLASI"
     gene            8487..9362
                     /locus_tag="ACZPCG_08705"
     CDS             8487..9362
                     /locus_tag="ACZPCG_08705"
                     /inference="COORDINATES: protein motif:HMM:NF012738.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1285171.1"
                     /translation="MKRKKSLLRCVEPYLYLLPAVVLFVLFVYYPFLKNEVLSFFTLD
                     KFRTVKGFAGIQNYVRVFGDEKFLLAVKNTLVYVAVTVPVSMVLGYFLALLCRKKRKS
                     SFVYEAMFALPMAVSASVVAMIFQLAYNPSMGIINKLTGLNVNWLSDQKLALISIMIV
                     QIWANVGYNFIFILSGVRGLSEEVMESAAIDGVSGIQMQTKIILPMLSPTLLFLLVKD
                     VAYAITTASFTLIMTEGGPNGATETMISYIYKKAITGTNYNAAFAATTVCFVLSALMM
                     ALSMALEGKKVHYDQ"
     gene            9352..10179
                     /locus_tag="ACZPCG_08710"
     CDS             9352..10179
                     /locus_tag="ACZPCG_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1285172.1"
                     /translation="MINKRKLQSLIYQLFCMFVGLAVISPIIYCVLVSFMQPSEILTK
                     EVHLIPDYIYLVNYIKAVTQTDIFRFMLNSFIVAFVSSVVRIITSSCAAYSFAFFEYP
                     GKKFLFYLTIGTMIIPTDVLLVQNYFTVSGMNLINTYTGMMVIFFVSGMNIFVIRQYF
                     LSYSLSLKEAAHIDGCGNLQFFLQILLPTTTPALVTVFISSFVGTWNTYLWPLIVTNV
                     NEMRTAQVAITMLNTEASSDFGVVMAAATIILIPSVLVLALFQRKIVGGMMGGAVKG"
BASE COUNT         2864 a         2162 c         2761 g         2475 t
ORIGIN      
        1 tgactacctt aacggctctg ccgaagaact ttggttataa tatgtccatc atgatccttg
       61 tatatgtggt tttgggcggg atcggaaata tccgcggttc tgtcatcgcc gccgttgttc
      121 tgtatatgct tccggagctt ctgagaggac tgagcaatta tcgtatgctg atctatgcag
      181 ttgttttgat ccttgccatg ctctttaact ctgcacccca gtttgtagtg ctcagagagc
      241 ggattgctca gaatttcaga aagaaagaga aggaggcggc ataatggcac tgctggaagt
      301 aaaaaattta agcatttcct ttggcggtct taaggccgtt gacgggttcc atatcgatat
      361 tgaaaaaggg cagctttacg gtctgatcgg accgaacgga gcgggaaaga ctaccgtgtt
      421 taaccttctt acaggcgttt ataagccaaa tgcgggaagt attgtgctgg acggcaaaaa
      481 tatcacagga aaaagcacca tccagatcaa tcaggagggt attgcaagaa cattccagaa
      541 catccgctta tttgcggata tgtctgtgct tgacaatgta aagacgggtc ttcacaatca
      601 tcacaggtat tctacactag aggggatttt ccggcttcca agataccata agacagaaaa
      661 ggaaatggat gaaaaagcca tgagcctatt agaagtattc gggctggcag gagagtgtga
      721 ttttaaggcc tccaatcttc cctatggaaa gcagcgaaag ctggaaattg ccagagcttt
      781 ggctacagat cccaagctgc tccttttaga cgagcctgcg gcaggcatga atcccaacga
      841 gacggcagag cttatggata ccatccgttt tgtcagagac aattttgata tgacgattct
      901 gctcattgag cacgatatga agctggtaag cggtatctgc gagaagctta ctgtcttaaa
      961 cttcggacag gtattaagag aaggctccac cagccaggtg ctccatgatc cggaagttgt
     1021 aaaagcatat cttggtgaat aggaggagag gatattatgg ctatgcttga agtcagagac
     1081 ctggaggtgt attacggtgt gatccaggcg ttaaagggaa tctcctttga tgtagaccag
     1141 ggagagatcg tggctttgat cggcgcaaat ggtgcgggaa agaccacaac actgcacacc
     1201 gttacaggcc ttttaagcgc caaggcagga aagatcgtct atgaaggaac ggatattacc
     1261 cgtgtacccg gttataagct ggtaagcatg ggaatcgccc atgtgcctga gggacggcgc
     1321 gtatttgcta ccctgacagt gcttcagaat ctgaaaatgg gcgcttacac ccgcaaggat
     1381 aaaaatgaga tggaagaaac gctaaagatg atctataagc gctttccccg tctggaggag
     1441 cggaaaaacc agctggcagg cactctttcc ggcggcgagc agcagatgct tgccatgggc
     1501 cgcgctctta tgagccaccc caggctgatc gttttggacg agccgtctat gggactttct
     1561 ccaatttatg taaatgagat ttttgatatc atccagaaga tcaatgagga tggaacgacc
     1621 gttctcctgg tagagcagaa tgcaaaaaag gccctttcca ttgcagacaa ggcctatgtt
     1681 ctggaaacag gtactattgc tctttccgga gatgcaaagg aactgatgaa caacgatcag
     1741 gtaaagaagg catatttaag cgagtaatac aagtgaaccc cctgtgcata tattataaaa
     1801 acatgcaagg gggtattttt atggacaatt ttaatgtata cagagatatt gaagccagaa
     1861 ccgggggaga aatctatatt ggagtggtgg ggccggtgag gacggggaaa tccaccttta
     1921 tcaaacgctt catggagctt ctggttcttc cggccatgga ggacgaacat ctgcgcgcca
     1981 tcagccggga tgaactgccc cagagcgctg cgggaaaaac cattatgaca acggaaccca
     2041 aatttatccc aaaggaggcg gcaaaaattt ccctggacga cgggattgaa gccagagtgc
     2101 gcttgattga ctgtgtgggc tttatggtag agggggccgc gggtcatgtg gagaatgagg
     2161 aggagcggct ggtaaagacg ccctggtttg accacgagat ccccttcacc caggctgcag
     2221 aacttggaac caggaaggtg atcggagatc attctaccat cggcattgtg gtgacgacag
     2281 acggcactat aggggatctg aaacgtccgg gatacatagc ggcagagcgc cagaccgtag
     2341 aggagcttaa aaaactggga aagccgtttg tcattctttt aaattccgta aagccctatt
     2401 cggaagaaac tgcccggctg gcaaaggaac tggaggagac gtatggggtg tctgtgcttc
     2461 cggtcaactg tgagcagctg aaaaaagagg acatttttca tattttagag tgcgttttaa
     2521 aggaatttcc ggttacggag atggattttc acattccaaa atggctggag atccttcctg
     2581 catcccactg gctgaaggct cgggtgatcc agacggcaaa agagatctta cagaaggtgg
     2641 agcacatgaa ggatgtggct ttaagaatac gggagatgaa ggatgaatcg ggagccatta
     2701 agtctttttc cgtgcggaaa atggaaatgg cagacggaag cgtggatctt gcagtggaaa
     2761 tggacgacag ctattactat cagatcttaa gcgattatgt gggtcttcct attgggggag
     2821 aatatgaact gatgcagacc ttaagcggtc tggcccggat gcagaaggag tatgaaaagg
     2881 tcaaaaatgc gctggctcag gcacggatca aggggtatgg cgtcatgatg ccagagcggg
     2941 aggaaatcct cttagacgaa ccacaggtga tccgccacgg aaacaaatac ggggtaaaaa
     3001 tgaaggcgca ggctccgtcc attaacctga tcaaggctca tattgagacg gaaattgccc
     3061 caatcgtggg aagtgaacag caggccagag acctcatcgc ctatattaaa tccaatgcgg
     3121 gagaaagcga ggagggcgta tgggatgcca atattttcgg gaagtccatt gaacagatcg
     3181 tagaggatgg aatccaggcc aagatctccc agatgacaga ggactgccag atgaagcttc
     3241 aggatacgct gcagaagatc atcaatgata gtaatggggg tatgatttgt attattatat
     3301 aaaattttta taaatattgc aaatcatact atgaatatgg tataatacct acaagttgac
     3361 agactattat actttttatt caaccacctt cagaggggag aatgcatatg aaactaacct
     3421 ttatcggagc agatcatgaa gtaacgggaa gctgtcattt tcttgaggca ggaggcagta
     3481 aggtgctgat cgactgcggc atggagcagg ggaataatcc atacgtcaat gcagagctgc
     3541 ctgtaagcta cagtgagatt gattacgttt ttctcacaca cgcccatatt gaccacgcag
     3601 gcctgctgcc gtggatttat gccagaggct ttaagggccg ggtcattacc acctatgcca
     3661 cgatggattt atgccagatc atgcttcgcg acagtgccca catccaggag atggaggcag
     3721 aatggaaaaa caggaaggct aaaagagccg gacgcggcga ggtagaggct ctttatacgg
     3781 tagaggatgc cgagggcgta ctgagccatt ttgagggcgt agcatatggt caggtgttta
     3841 agctgaatga caatctgacc ctccggttta ccgatgtggg ccatctgctt ggctctgctt
     3901 ccattgaaat ctgggccaaa gagggggatg aggagcgaaa gatcgtattt tccggcgata
     3961 tcggaaataa aagcaaaccg ctgatccgtg accctcagta tatttcagag gcagattatg
     4021 tggtgatgga atccacctat ggaaacagat accacaaaaa gaccactacc tatgtagaga
     4081 gcctgacaag gatcatccag gaaacgctgg accgttcagg gaatgtggtg attcctgcct
     4141 ttgccgtagg ccgtacccag gagcttcttt atatgatccg ccatatcaag gaacataatc
     4201 tggttcacgg ccacgatgga tttctggtgt atgtggacag tcccctggct gtggaggcaa
     4261 cacaggtatt tacggaaaac cttctggact gctatgatga tgagacaaag gcactggtag
     4321 agcgggggat caaccccatt gattttaaag ggctgaagct ttcagttacc agtgatgaat
     4381 ccaaaaacat caattttgac atgacgccca aggttattat ttccgcagcc ggtatgtgcg
     4441 atgcaggccg tatccgccat catttaaagc ataacctgtg gcgcccggag tgttcgatcg
     4501 tatttgccgg ctatcaggcc gagggggcct taggaagggt tcttcaggat ggcgcgccgg
     4561 cggtaaagat tttcggagag gaaattgacg tgcgggctca cattgaaacc atagacggca
     4621 tcagcggcca tgcagacaga gacggtctcc ttgcctggat ctcagccttt gaaaaacagc
     4681 cctcccatgt atttgtagta catggaaatg aggaatcctg caccgccttt acagaggctc
     4741 ttcaaaacca gctcaggctg acggcggatg ccccgtactc cggttcccag tttgacctta
     4801 taaagaactg ctggatctgc cagacagagc cggttcttgt tgagaaggag acagaggcca
     4861 gacgcaaggc cacaggcgtt tataccagac tggtagacgc aggcagccgg cttttggatg
     4921 tgatcagcca taaccagggc ggtgccaacc gggatctggc ccggtttaca gagcagattc
     4981 tggctctgtg cgagaagtgg gaccggtaaa gtgccgcttc ctatcaatga acaacaggag
     5041 agacaaaaaa caatgacaga agtacagatc tatacagacg gttccgcccg gggaaacccg
     5101 gacggtcccg gcggctacgg cgtagtaatg cactatacag accggaccgg aaagcgctac
     5161 gaaaaaacca tgtccggtgg ctatgtccgc accaccaata accgcatgga gcttatggcg
     5221 gcgattgcag gcctggaagc cctgaaccgt ccctgcgcgg tttcccttta ttccgattcc
     5281 aaatatctga cagatgcctt taacaaacac tggatcgaag gctgggtggc aaaaaactgg
     5341 gatcggggaa agagcgggcc ggtaaaaaat attgacctgt ggaagcgcct tttaaaagca
     5401 aaggagcctc atcaggtcac ctttatatgg gtaaagggcc atgcgggcca tccggaaaat
     5461 gaacgctgcg accagctggc tacgacggct gccgacggaa ctgatctgat gatcgatgaa
     5521 ggactccaat aggagtcctt tttagatatt acagatatct tacaatgtat tactcctttg
     5581 aaaatcgtat tcacaattat ccgtttttgt gataatgtat cactggaaag tgaaattgag
     5641 tcagacaggg atgatggaat atgaaagata aaaggatatg gctggtgatt ttggggattc
     5701 tggtaatcgg cattctggtg accggttata ccaggtctgt agtaaaaaaa caggcagaca
     5761 gtgccgcctt tgagggagaa atatccacag aggcttcttt ggggccgttt aaggaacaga
     5821 cagaggaaga ggctcagaag gaggagagcg gcgctgtgca ggaacaggct caggcagata
     5881 cggcagtccc ggaaacggaa caaaaggaag aagaggcggg agaacccgct gcaatctctg
     5941 agcctgtccc tgcaatggcc cggtcagcag tcccggaaag tgggagagcg gccacagatt
     6001 cacaggcaga cagtccgtgt gaaggcggac tttatagaca gcgtctgaat gatctggacg
     6061 cacagatcca gaaaatgcag gagcaggaaa gggatccgaa cgtatactcc attaaaacat
     6121 ctgcggagac cgaagtcaaa atgtgggaca gggagctgaa tgcagtgtac aatgcccttt
     6181 tgggaatcct ccctcaggag gaggcggagg aacttgcaaa ggagcagaag gagtggctca
     6241 taaatcgtga aacggctgcc ggacagtctg gaaaggcgga aggggtagga agcatcagtt
     6301 atgcagccag cctggtggat ttaaccagaa atcgcgccta tgaactggca gaccgatacg
     6361 agaaagaggc ctgcgggccg caggatgctg aaaacgaaag cgatacggct gttttgccat
     6421 aagcagtgag atatattctt ataagggcgt ctgagaaatc agacgtcttt ttgcatttta
     6481 caactttata aaaaatagtt ggaaaaaacc aaaaaactgt tataatgaaa tcaaaatacc
     6541 catatggagg agagaaatga ataaagcaga ggaacgatat caggaaatca tagaaatatt
     6601 aaaggaaaat cccattgtta cggtggcgga ttttgcagag cgtctttctg tttctatgga
     6661 aaccatcaga aaggacttaa atatcctgtc ggaacagggc aagatcgtcc gtgtccatgg
     6721 cggggccgcc cttgcaggaa atacggaaat ggcttctccc tttcagttca gagaaggcgt
     6781 gcgccgcagg gaaaagaaga agctggggaa agaggcggta aagctgatac agaaggggga
     6841 taccctgatc atagagagca gcaccacaac ggcagaattt gtaaaggagc ttgtaaaagt
     6901 tccggaactg ctgaaaaccc tgacgattgt tacaaattca attcacattc ttacattggt
     6961 ggagatgggg aagctgtgta agagagtgtt tcttttaggc ggctggatca atgagagaga
     7021 gggggctacc aaggggcagt ttatggcgga acagttaaaa agcttccatg tggataaatg
     7081 catcctcagc ggggcagctc ttggaaagaa cctgatcctg tcctcctact atgagagcga
     7141 tatgcatttt cagaagctgg ctatggaaag cgcgggagag accattcttt tgctggaagc
     7201 gggaaaatac ccggaggctg ctgtgttttc tgtaacggct gccaccaatt ttgactatat
     7261 ggtaagcaat gtgcccttta aaaaagagga taaggagcgc tttgcagcgg ccggccttca
     7321 tctgatctat gtgcctactg gttccgaaga agcaactgat aatgattgaa atgtgaaaaa
     7381 acaaattaac tatcgtaaaa atattgcaaa aaaccaaaaa taatattgac tattggcgaa
     7441 aataatgata aaaagtaatt aaaagttgtc gaaacaaaat aaaagagaaa aggagtcata
     7501 ccatgatacc aaatcccttt ggcttttcct attcagatga tattgacatt tcagaaatgg
     7561 aacagtccat cgcccgttta aaacggcctc aggggcggat tgacgttgtg ctggatacgg
     7621 atacctttaa tgaggtggac gatcagtttg ctctggcgta cctgcttcag tcagaggatc
     7681 gtctgaacct gaaggcaatt tatgcagctc cctttgacaa ccatcattcc aatggtccca
     7741 aagacggcat ggagcgcagc tatgaggaga ttttcagagt gctggcgatg ctgaaaaaaa
     7801 gccagtatga agaatgtacc tacaaaggtg cagagcagtt tttagacagc gaggagactc
     7861 ctgctgtttc agcggctgca gagcatctgt gcagtctggc aatggagtat acaaaggaag
     7921 atcccctcta tgtgattgcc atcgcagcct gtaccaatat tgcctccgcc cttcttatga
     7981 agcctgagat taaggacagg atctttgtgg tatggctggg aggcctgtca ttggactggc
     8041 atgataattt ttcctttaat gcaaagcagg atgtggcggc ggcccgtgtg cttcttggaa
     8101 gcggagttcc cctggtgctt ctgccgggca gaggggtggt agaccatttt tcaaccacag
     8161 ggcctgagct ggagtactgg ctgcggggga aaaatgagtt ctgcgattac atcattgaca
     8221 agacccgtca tgaggcagag attgtaagcg gagaaacctg ctggagccgg ccgatttcag
     8281 acgtaacggc agtggcctgg ctgcttgata cctcctttat gctggaccgt ctggaaccaa
     8341 gccctattat ggagtatgac caccattatg caaaggataa gcggcgccat tttatccgat
     8401 atgtctacag catcaaccgt gaccgtctga tgaaggatct gtttgataag ctggcatcta
     8461 tctgatatcg aaagaggaaa tatgaaatga aacggaagaa aagcctttta agatgcgttg
     8521 agccctattt atacctgctc ccggctgtgg tgctgtttgt cctgtttgtg tattacccgt
     8581 ttttaaaaaa tgaggtttta agctttttca cccttgataa attcaggaca gtaaagggat
     8641 ttgcgggaat ccagaactat gtaagggtct ttggagatga gaagttttta ctggctgtaa
     8701 aaaacaccct ggtctatgtg gctgtgactg tgcctgtatc catggttctg gggtattttc
     8761 tggctctgct gtgcagaaaa aagagaaaga gctcctttgt gtatgaagcc atgtttgccc
     8821 ttcccatggc agtttcggca tcggttgttg ccatgatctt tcagctggcc tacaatccgt
     8881 ccatgggtat tatcaataag ctcaccggac tgaatgtaaa ctggctgagc gatcagaagc
     8941 tggccttgat cagcattatg atcgtgcaga tctgggccaa tgtagggtac aattttattt
     9001 tcatcttatc cggcgtgaga gggctttcgg aggaagtgat ggagagcgcg gccattgacg
     9061 gcgtaagcgg catccagatg cagacaaaga tcatccttcc catgctttcc cccaccctgc
     9121 tgtttctgct ggtaaaagat gtggcctatg ccataaccac agccagcttt acgctgatca
     9181 tgactgaggg cgggcccaac ggagccactg aaaccatgat ctcttatatt tacaaaaaag
     9241 ccattacggg aaccaattac aacgcggcct ttgcggctac gaccgtatgc tttgtgctgt
     9301 cggctcttat gatggcatta agcatggctt tggagggaaa gaaggtgcat tatgatcaat
     9361 aagagaaagc tgcagagcct gatttaccag ctgttttgta tgtttgtggg actggctgtc
     9421 atttcaccga ttatatactg cgtcctggtt tcctttatgc agccgtcgga gatcctgaca
     9481 aaagaagtgc atctgatacc ggattacatt tatctggtaa actatataaa ggctgtgact
     9541 cagacggata ttttccgctt tatgctcaac tcctttatcg tagcctttgt atcaagcgta
     9601 gtccggatca ttacctccag ctgcgcggcc tattccttcg cattttttga gtatccggga
     9661 aaaaagtttt tattttatct caccatcggt actatgatca ttcccacgga tgtgctgctg
     9721 gtacagaatt attttaccgt ttccggaatg aacctgatca atacctatac gggaatgatg
     9781 gtgattttct ttgtttccgg aatgaatatt tttgtgatcc gccagtattt tttgagctat
     9841 tccctgtctt taaaggaagc ggcccatatt gacggctgtg gaaacctgca gtttttcctg
     9901 cagatccttc tgccgaccac cactccggcc ctggttacgg ttttcatttc ctcctttgtg
     9961 ggaacctgga atacgtacct gtggcccctg atcgtgacaa atgtaaatga aatgagaacg
    10021 gcccaggtag ccattaccat gctcaataca gaggcgtcca gtgatttcgg cgttgtgatg
    10081 gcagcagcga ccattatcct gatcccctcc gttctggtgc ttgcgctttt ccagcgcaag
    10141 atcgtaggcg gcatgatggg cggagctgta aagggctgat gctacagacg taaacgtaca
    10201 taatgacatg taagaaagga atacaagatg atgaaaaaga aagtgattgc tacaaccatt
    10261 ct
//
LOCUS       DBMDUH010000093        10228 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_3188_length_10228_cov_5.473289, whole genome shotgun sequence.
ACCESSION   DBMDUH010000093 DBMDUH010000000
VERSION     DBMDUH010000093.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 10228)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10228)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10228
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3188_length_10228_cov_5.473289"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1269
                     /locus_tag="ACZPCG_08715"
     CDS             <1..1269
                     /locus_tag="ACZPCG_08715"
                     /inference="COORDINATES: protein motif:HMM:NF024242.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1285173.1"
                     /translation="SAMRLGAIDYLIKLNLEENELVSLIQSAQKMLSKRPKRLQPPSH
                     SEDFKRNFIKEMLQMNADKSLFAKQLPENFALNDNCFYRILCFSLKDSEAVPAGSGDL
                     PQNYLDNICMEQFPERADLCAHHLNPNTTILYVEYRDGMDQDILKKKCQSILNSVRRY
                     LNRQLTIGISAPHRNIMHLAAAREEAMESLSFQVSGIDTSIHFYTDFLNADSLKHQLE
                     LLNTESTFFEEAENLMLQIQKFLSQSIAKPGDARQICFLLISRLYGFDSKIRDFFIQW
                     FGREYTSLSQFDQMKNQELVSWLLHLTQGIDHYSHQYMTEIYRYKVKKAREYIFENRF
                     QKLSLNEVATVLEITPSYLSRIFKKVTHQSFSDYVAEVKIEEAKSLLLKDNNRIYEVS
                     SMLGYDDPYYFSKVFKKITQMTPSEFIARN"
     gene            complement(1271..2101)
                     /locus_tag="ACZPCG_08720"
     CDS             complement(1271..2101)
                     /locus_tag="ACZPCG_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008722616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1285174.1"
                     /translation="MKTSRINIKTICLYGLLVLLSITILLPFLWMVSSSLKTSQEVFS
                     VPMKWLPEEPVWENFREIWTKIPLLMFFKNSFKLAIIITVLQVLTSSFAAYPFAKMKF
                     PGKDIIFLGYIATIAVPWQVYMVPQFIMIRKMQLADTHLSLILMQAFTAFGVFLMRQS
                     YLSIPNELSEAARIDGLSEFGIYSRIMLPLSKSAIATLIIFSFVNVWNDYMGPMIYLN
                     STELKTIQLGLRMFITQYSADYNLIMAAALISLIPVGALFLSMQRFFIEGVATSGLKG
                     "
     gene            complement(2120..3001)
                     /locus_tag="ACZPCG_08725"
     CDS             complement(2120..3001)
                     /locus_tag="ACZPCG_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009445006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1285175.1"
                     /translation="MSKRKVKNIITAYTFLLPNFVGFLLFTLIPIVCSMGLSFMEWDS
                     ANPMKFVGLANFAKLLEDETFSISLKNTIYYSVFTVPLTMAAALGLALILNQKLKGIN
                     MFRTVFFFPHVASLVAVAVVWNLLFHPTLGPINNILRAMGVANPPGWTSTIDWAMPVI
                     IIVSIWKSMGYYMILYLSGLQAIPRELYEAAKVDGANTFQRFRNITLPMLTPTTFFVS
                     IMLTIACFKVFDLISVMTNGGPGRATNVLVYHIYNTAFINYEFGYASAISMVLFAIVL
                     VITVVQFRAEKKWVSYM"
     gene            complement(3096..4427)
                     /locus_tag="ACZPCG_08730"
     CDS             complement(3096..4427)
                     /locus_tag="ACZPCG_08730"
                     /inference="COORDINATES: protein motif:HMM:NF024808.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1285176.1"
                     /translation="MMRHNKLWKKAMALTCAAACAATTLAGCGSSTQAEKPSEKTEAQ
                     GESGAAEGQTEITFTVWDYETTDYWKKLVEAFEAENPDIKVKVMDIGATDYDTKIPVL
                     LSSGDTSDVITIKSMNIYNSLVEKNQLMPLDDKVKESGVDMAPYQGSDEGIRIDDKLY
                     GLPFRNDYYLLYYNKTLFDQAGVEYPKNDMTWDEYRELAKKMTSGEGNDKIYGGYCHT
                     WPGSLYRWGLSEEHSMADGEYSFLKPVYELFLGMQNEDMSAMDYGTLKTGNVHYSGPF
                     YKEQVAMELMGTWFANMIINTKNKGETSVEWGAVRAPHFEGQEPGTAVSNPTPIAINK
                     NAAHPEEAWRFVEFMCTEPGANILAGLGTLPAYQNEKTIDTLVNVEGMPEGFREAIAA
                     DNFVLECQMIPQAGALEQIIAEEHDLIMLGANSIDDGLANMAKRVNDLLSK"
     gene            4728..5870
                     /locus_tag="ACZPCG_08735"
     CDS             4728..5870
                     /locus_tag="ACZPCG_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HIR1285177.1"
                     /translation="MTSKTQWVNEIYEKMEQKLYAECARMGEKIPYWTVGGVYKEDYA
                     KKDIYWWTNGFWSGILWQLYHATKNELYLETARKNEDLLDQALHGFIGLHHDTGFMWL
                     HSAVADYRLTGSEEAKVRGLNAASVLAGRYNPNGKFIRAWNPECVEEGEDCTGWIIVD
                     SMMNIPLLYWAGQITGDPRFQQIAVNHADTVASYLVREDGSCGHIACINPDTGELEHI
                     LGGQGYSETSSWSRGQSWILYGFALSYRHTKNKKYLDIAKKTSHYFISNIALTGYIPL
                     CDFRQPASAAYTDTSAGLCAACGLLEIAEHVDECEKNLYRTYAELILKHTAETCCDWN
                     PDTDGIVQNCKVAFHNDRREQTDLIYADYFLTEAVLRLLGKDFLIW"
     gene            5906..7582
                     /locus_tag="ACZPCG_08740"
     CDS             5906..7582
                     /locus_tag="ACZPCG_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HIR1285178.1"
                     /translation="MTKHEYSQLLMQLLTPTLPFYSPQKARLCPGSSAAVYDMAAAGA
                     EGFARPLWGLVPFWAGGGSCPEFEQLYLSGLAAGTDPENPEYWGGFQDIDQRFVEMAA
                     IAYGILMTPQKLWDPLSAKEKTNLAKWLDGINHYACPPCNWMFFGVLVNIALKSVGFP
                     YSEQVLSDYLDYIESCYLGGGWYLDGQNGEKDYYISFAFHYYSMIYCRFMRENDPDRC
                     ALYEERAKLFAADFINWFAEDGSALAYGRSLTYRFAQVSFFSMCAACELEVVPYGIMK
                     GLIERHLRFWTEHPIVDNSGLLTIGYGYSNLLMSEQYNAPGSPYWAMKAFAFLSLPDE
                     HPFWKTEAAPLPDLPTLTAQTYGGLLIQRLHGDVTAYTCGRTLPHHHVLTEEKYSKFA
                     YSSRFAFSVPRSMRSLAEAAPDSMLALDINHMIYVRGMVSNPQLTDHSVSSEWSPYEG
                     IRIKTVIIPDSTGHKRIHTIESDRCFTAYDCSFAFPDTYTVSQEQSCIRVQAPDGRAY
                     CASAVKDDPAAMCINASPNTNLMVPKTIIPCICYEIQKGTQVIETRFWEE"
     gene            7806..8840
                     /locus_tag="ACZPCG_08745"
     CDS             7806..8840
                     /locus_tag="ACZPCG_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285179.1"
                     /translation="MFFGGIAERTLSIKLPEFRKQIETYSIEELRYLTAELYKAIPKK
                     IKEEKEIDPLVLSVPEHFKENGTGKASSPSKVKKAPDLGALESEIELFLENAYAQNYF
                     APNRFVPKHERPKWRFKVKNYIKTLRDHYTEGEEAETAALLLEKLYRMLCYGCCYYIF
                     STTDPFQSIGMRQNELLDLVIKKSFACGVTSERICKMEEISTLSGLSYDMLSGSLLSV
                     LAANLKTADMKETAIAEAKKLRQKIVSIRYSDREQKNSLTTLILMIHFSLYEYEEGIR
                     DFKEKYLEPDKEILYYVLLSHMFFYDLKNYWVREYKTALNQGISLRKSLMEIYEYLME
                     HGEFPESFYL"
     gene            8863..9276
                     /locus_tag="ACZPCG_08750"
     CDS             8863..9276
                     /locus_tag="ACZPCG_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009262685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR1285180.1"
                     /translation="MWLFFALLSALFAALTSILAKLGIDGVNSNLATAIRTVVVVVMA
                     WGMVFLTHAQNGIFSIGRKSWIFLILSGLATGASWLCYYRALQLGDASKVVPVDKLSV
                     VMTLILAFVFLHEDFTAKSLIGCILIGAGTLVMVL"
     gene            complement(9540..9758)
                     /locus_tag="ACZPCG_08755"
     CDS             complement(9540..9758)
                     /locus_tag="ACZPCG_08755"
                     /inference="COORDINATES: protein motif:HMM:NF024080.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3791 domain-containing protein"
                     /protein_id="HIR1285181.1"
                     /translation="MNRFDSQSDFAAKKYAWIIERFSKKKNLSFDEALKFFYCSALYP
                     LLCEGVSDLHCMSDEYLTEELIFEYEET"
     gene            complement(9745..10175)
                     /locus_tag="ACZPCG_08760"
                     /pseudo
     CDS             complement(9745..10175)
                     /locus_tag="ACZPCG_08760"
                     /inference="COORDINATES: protein motif:HMM:NF024550.6"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
BASE COUNT         2892 a         2383 c         2109 g         2844 t
ORIGIN      
        1 tctgccatgc gcttaggcgc cattgactat ctgatcaagc tgaatctgga ggaaaatgaa
       61 ctcgtttccc tgattcagtc tgctcagaaa atgctttcaa agcgccccaa acgtctgcag
      121 ccgccgtctc actcagaaga ttttaaacga aatttcatta aagaaatgct tcagatgaac
      181 gcagacaaaa gcctgtttgc caaacagctg ccggaaaatt ttgctctgaa tgacaactgc
      241 ttttaccgta ttttatgctt cagtctgaag gattctgaag ccgtgcccgc cggatcggga
      301 gatcttccgc agaattacct tgataatatc tgtatggaac agtttccgga acgtgcagac
      361 ctttgcgcac accatttgaa tccaaatacg acgattctgt atgtggaata ccgcgacggg
      421 atggatcagg atattctaaa aaagaaatgc cagtccatcc taaacagcgt ccgccgttat
      481 ctgaaccgac agcttaccat aggaatcagc gcgccccatc gcaatatcat gcatcttgcc
      541 gctgcaaggg aagaagcgat ggaaagcctc tctttccagg tcagcggcat tgatacctcc
      601 atccattttt atacggactt tttaaacgca gattcactga agcatcagct ggaacttctg
      661 aatacggaaa gtacgttctt tgaagaagct gaaaacctta tgctgcagat tcagaaattt
      721 ctgtctcaga gcatcgcaaa gccgggggac gccagacaga tctgctttct tctgatctcc
      781 cgtctgtatg gatttgactc aaaaatccgg gattttttta tccaatggtt cggcagggaa
      841 tatacctccc tctcccagtt tgaccaaatg aaaaaccagg agcttgtgtc ctggcttctc
      901 catctgactc agggaatcga ccactacagt catcagtata tgactgagat ttaccgctac
      961 aaagtaaaaa aagccaggga atacattttc gagaaccggt ttcagaaatt atctcttaat
     1021 gaagtggcca ctgttctgga aattactccc agctatttaa gcaggatctt taaaaaggtg
     1081 acgcaccagt ccttttccga ttatgtggca gaagtaaaaa tagaagaagc gaaatcgctt
     1141 cttctaaagg ataataaccg gatctatgaa gtttccagta tgcttggata tgatgacccc
     1201 tactatttca gcaaggtatt taaaaaaatt actcagatga ctcccagtga atttattgcg
     1261 agaaactgaa tcatcccttc aggccgctgg ttgccacgcc ttcaataaag aagcgctgca
     1321 tacttaagaa taacgctccc accggaatca gagaaatcag agcagctgcc ataatcagat
     1381 tatagtccgc agaatactgt gtgatgaaca tacgaagacc cagctgaatg gttttcagtt
     1441 cggttgaatt taagtaaatc atgggaccca tgtaatcgtt ccatacgttt acaaagctga
     1501 aaataatcag tgttgcaatc gcagatttgg acagaggcag cataatcctg ctgtaaattc
     1561 caaactcgct cagaccatct atgcgggcag cctctgaaag ctcatttgga atggaaagat
     1621 aggactgtct cataaggaac acgccaaatg cagtaaaggc ctgcatgagg atcagggaca
     1681 gatgggtgtc tgccagctgc attttacgga tcatgataaa ctgaggcacc atgtacacct
     1741 gccacggaac ggcgatcgta gcaatgtagc ccaaaaatat aatatctttt cccggaaact
     1801 tcatttttgc aaacggatag gctgcaaagc tgctggttaa tacctgaaga accgtaataa
     1861 taattgccag cttaaaacta tttttaaaaa acataagcag cggaatcttt gtccagatct
     1921 cccggaaatt ctcccatacc ggttcttcgg gcagccactt catgggaaca gagaacactt
     1981 cctggctggt ttttaatgat gaggatacca tccataaaaa cggaagcaga atggtgatgc
     2041 ttaaaagtac aaggagtcca tacagacata tggtctttat attgattctt gatgttttca
     2101 ttttcaatcg ttcctccttt tacatatagc taacccattt tttctcagct ctgaactgga
     2161 ccacagtaat aacaagtacg attgcaaaca gaaccatgga aattgcgctt gcatatccaa
     2221 actcataatt aataaatgct gtgttgtaaa tatggtatac aagtacgttg gttgcacgtc
     2281 ccggtccgcc gttcgtcatg acggaaatca ggtcaaagac cttaaagcag gcaatggtca
     2341 gcataatgct tacgaaaaac gtagtcggtg tcagcatagg cagcgtgata tttctgaacc
     2401 gctgaaatgt attggcgccg tctacctttg ctgcctcata aagctctctt ggaatcgcct
     2461 gcaaaccgga taaatacaga atcatatagt atcccatact tttccatata cttacaataa
     2521 tgataaccgg cattgcccaa tcaatcgtag aagtccatcc tggaggattt gcaactccca
     2581 ttgcacgcag aatattgtta atcgggccaa gggttggatg aaacagcaga ttccatacaa
     2641 ctgccaccgc taccagagac gctacatgag ggaagaaaaa tacggttctg aacatgttga
     2701 tgcccttcag cttctggttt aagatcagag caagccccag agctgctgcc atggtaagcg
     2761 gtactgtaaa tacagaataa tagatggtat ttttcaggga aatactaaat gtttcatcct
     2821 ctaaaagctt ggcaaaatta gcaagcccta caaatttcat cggatttgca gaatcccatt
     2881 ccataaaact aagtcccatg gagcatacga tggggatcag cgtaaacagc agaaatccca
     2941 caaaattcgg caaaagaaat gtataggcag taattatatt ttttactttt cgttttgaca
     3001 tatctgcgcg ctgtaaaaca gctcctcctt cttttctctg atggttcaaa gtcctccgaa
     3061 tcaaagatcc agaggacttt ttttcagtct gcttcttatt tacttaacag atcatttaca
     3121 cgctttgcca tatttgccag accatcatca atggaatttg ctcccagcat gatcaggtca
     3181 tgctcttctg caataatctg ctccagagcg cctgcctgag ggatcatctg gcattccagt
     3241 acaaaattat ctgcagcgat cgcttcacgg aagccttccg gcattccctc aacatttacc
     3301 agagtatcga ttgtcttttc gttctggtat gctggcaacg ttcccagacc tgccagaata
     3361 ttggcacccg gctctgtaca cataaactct acaaatctcc atgcctcttc cggatgagct
     3421 gcattcttgt tgattgcgat cggagttggg ttgctgacag ctgtaccagg ctcctggccc
     3481 tcaaagtgcg gtgctcttac agcgccccat tcaacagagg tctcaccctt gttcttggtg
     3541 ttgatgatca tatttgcaaa ccatgttccc attaactcca ttgctacctg ctccttgtag
     3601 aacgggccgc tgtagtgaac gtttcctgtc tttaatgtac cgtaatccat agcggacata
     3661 tcctcattct gcatgccgag gaataattca taaacaggct ttaagaagct gtactcaccg
     3721 tcagccatac tgtgttcttc gcttaagccc caacgatata aggaacccgg ccatgtatgg
     3781 cagtatccac cgtaaatctt atcatttcct tctccgctgg tcatcttctt tgcaagctct
     3841 ctgtactcat cccaggtcat atcgttcttt ggatactcaa cacccgcctg atcgaacaga
     3901 gtcttgttgt aatacagcag atagtagtca ttacggaatg gaaggccgta cagcttgtca
     3961 tcgatgcgga ttccttcatc cgatccctga taaggagcca tatcaacgcc gctctccttc
     4021 accttatcat ccagaggcat cagctgattt ttctccacca gactgttgta aatgttcata
     4081 ctcttaatag taataacatc agatgtatca ccggaagaaa gcagtacagg aatctttgta
     4141 tcgtaatcag tagccccgat atccattacc tttaccttaa tatctggatt ttctgcctca
     4201 aatgcctcta ccagtttttt ccagtagtcg gttgtctcat aatcccatac ggtaaatgta
     4261 atctctgtct gtccctctgc agcgccgctc tccccctgag cctctgtttt ttcagaaggc
     4321 ttttcagcct gtgtagagct gccgcagcct gccagagttg ttgcagcaca tgccgctgca
     4381 caggtaagtg ccattgcttt tttccataac ttattgtgtc tcatcatatc ctcctctttc
     4441 catttcagtc acatttgatg cctttattat agataaaatt tttttatttc tgaatggaat
     4501 atctgttatt atattgtttt aatataaact catccatgta ttctcccgtt ttttattgaa
     4561 atttcatata ctattcagga ttattttgca cttttcattt ttaccatgta taaaatgtta
     4621 cgttatacgg atgttttctc cattttcttt attcgttttt tcttttatac ttgaatttga
     4681 aaataatggg cgcatcatgc gcagtacatt caaggaggta tcaacttatg acatcaaaaa
     4741 cacaatgggt aaatgagatt tacgaaaaaa tggaacagaa gctgtatgcg gaatgtgcaa
     4801 gaatgggtga aaaaattccc tactggacgg tcggtggtgt atacaaagaa gattatgcca
     4861 aaaaagacat ctactggtgg accaacggct tctggagcgg tatcctctgg cagctttatc
     4921 atgccacaaa aaatgaactt tatctggaaa cagcccgaaa aaatgaagat ttactggatc
     4981 aggcccttca cggcttcatc ggccttcacc acgataccgg tttcatgtgg ctccacagcg
     5041 ctgtcgctga ttaccgcctc acaggttccg aagaagccaa agtccgcggc ctcaatgcag
     5101 cctctgttct ggccggaaga tacaatccaa acggaaaatt tatccgggca tggaatcctg
     5161 aatgtgtaga agaaggagaa gactgcaccg gatggatcat cgtagattcc atgatgaata
     5221 ttcccctcct gtattgggca ggccagatca ccggagatcc ccgcttccag cagattgctg
     5281 taaatcacgc agacacggta gcctcttatc tggtgcgtga ggatggctcc tgcggccata
     5341 ttgcctgcat caatccggat accggagaac tggaacacat tctgggcgga caaggctact
     5401 ctgaaacttc ctcctggtca cggggtcagt catggatcct gtacggcttt gcactgtcct
     5461 accgtcatac caaaaataaa aaatatctgg acattgccaa aaaaacatcc cactacttta
     5521 tctccaatat tgcactgacc ggttatatcc ctctgtgcga tttcagacag ccggcctctg
     5581 ccgcttatac agacacatct gccggacttt gcgctgcctg cggactgtta gaaattgcag
     5641 agcatgtgga tgagtgtgaa aaaaatcttt accgcaccta tgcagaactc atcctcaaac
     5701 ataccgctga aacctgctgt gactggaatc ccgatacaga tgggattgta cagaactgta
     5761 aggtggcatt tcacaatgac cgcagggaac agacggatct gatttatgca gattattttc
     5821 tcacagaagc agtgctgcga ctgctgggaa aggacttcct gatctggtaa tatccaaaat
     5881 acacaaattc aggagattaa aaattatgac aaaacacgaa tattcccagc ttctcatgca
     5941 gcttttaacc ccaacgcttc cattttactc tcctcaaaaa gccagactct gcccaggcag
     6001 ctctgccgca gtatacgaca tggcagcagc cggagccgaa ggctttgcca gaccactctg
     6061 gggcctggtc cccttctggg ccggaggagg cagctgtcct gaatttgagc agctgtatct
     6121 gtcaggcctt gccgcaggca ccgatcctga aaacccggaa tattggggcg gctttcagga
     6181 tattgaccag cgctttgtgg aaatggctgc catcgcctac ggcatactga tgacacctca
     6241 aaaactgtgg gaccctctct ctgcaaagga aaagacaaat ctggcaaaat ggctggacgg
     6301 gatcaaccac tatgcctgcc ccccatgcaa ctggatgttt ttcggcgttc tggtaaatat
     6361 tgcccttaaa tcggtcggtt ttccttattc agaacaggtt ctgtccgact acctggatta
     6421 cattgagagc tgctatctgg gaggcggctg gtatctggac ggacaaaacg gtgaaaaaga
     6481 ctattatatt tcctttgcct tccattatta cagtatgatt tactgccggt ttatgaggga
     6541 aaatgatcct gaccgctgtg cactttatga agagcgggca aaattatttg cagcagattt
     6601 tatcaactgg tttgcagagg acggctctgc cctggcctac ggccgttctc tgacttaccg
     6661 ctttgctcag gtatcctttt tctccatgtg cgctgcctgt gaactggaag ttgttcccta
     6721 tgggataatg aaggggctta tcgaacgcca tctgcgcttt tggacggagc atcccattgt
     6781 tgacaacagc ggactcttga ccatcggcta cggctattcc aacctgctca tgtctgaaca
     6841 gtacaacgct cccggatctc cctactgggc aatgaaggcc ttcgccttcc tctcccttcc
     6901 ggacgagcat cccttctgga agacagaagc ggctcccctt cccgatcttc caacgctgac
     6961 tgctcagacc tatggcggtc ttttaatcca gaggcttcac ggcgatgtta ccgcctatac
     7021 ctgcggacgg acgctgcctc atcaccatgt ccttacggaa gaaaaatatt caaaatttgc
     7081 ctattccagc cgttttgcct tcagcgttcc gcgttccatg agaagtctgg ctgaagccgc
     7141 acctgacagt atgcttgccc tcgatatcaa ccatatgatc tatgtccgcg gaatggtttc
     7201 caatccccag cttacagacc attcggtatc ctctgagtgg tctccctatg aaggaatccg
     7261 gataaaaaca gtcattattc ctgacagtac cggtcataag cggatccata ccattgaaag
     7321 cgaccgctgt ttcactgcat atgactgcag ctttgcattt cccgatacct atacagtttc
     7381 tcaggaacag agctgtattc gcgttcaggc tccggatgga cgggcatact gcgcctctgc
     7441 cgttaaagac gatccggccg ccatgtgcat taacgcatct ccgaacacaa acctgatggt
     7501 tcccaaaacc atcatcccct gtatctgtta tgagatacaa aagggaacgc aggtaattga
     7561 aactcggttt tgggaagaat agcatcaaat gctgccgaag aaaaagtaat caaagagcat
     7621 cctgcgtaaa aatgcgtggg atgctttttg tatttgaata cttgagttat agctaaaagt
     7681 aagttaaact tagtcataga atttagtcat aaaagctttt agaaaggcga aatgaaatga
     7741 tttattaggt atgcaaagcg gtatttaaca taacaaaaat agtgctaaat cctcaaaacg
     7801 ggaccttgtt ttttggagga atagcagaaa ggactttatc gataaaactt cctgagttca
     7861 gaaaacaaat agaaacatat tccatagaag agctgcgcta tctgaccgca gaactgtaca
     7921 aagcgatccc gaagaaaata aaagaggaaa aggagattga ccccctggtt ctgtctgttc
     7981 cggagcattt caaagaaaat ggaacaggca aggcctcttc cccctctaaa gtaaaaaaag
     8041 ccccggatct tggagccctg gaatcagaga ttgaactttt tcttgaaaat gcctatgcac
     8101 agaattattt tgccccaaac cgcttcgttc caaagcatga gcggcccaaa tggcgtttta
     8161 aggtgaaaaa ctatattaaa acccttcggg atcattatac agaaggggag gaggctgaga
     8221 ctgcggccct tcttctggaa aagctgtacc gtatgctgtg ttacggctgc tgctattata
     8281 ttttcagtac aaccgatcct ttccagtcca tcggaatgag gcagaatgaa cttctggatc
     8341 ttgtgatcaa aaaaagtttt gcctgcggag tcaccagtga acgcatctgc aaaatggaag
     8401 aaatttccac attaagcgga ttaagctatg atatgctttc tggatccctt ctttctgtcc
     8461 ttgctgcaaa tttaaagact gcagatatga aggaaactgc gattgcagag gcaaaaaaac
     8521 tgagacagaa aattgtcagc atacgttatt ccgaccgtga acagaaaaac agcttaacta
     8581 ctctgattct gatgatccat ttctctctgt acgaatatga ggaggggatc agggatttta
     8641 aggaaaagta tctggaaccg gataaagaga tcttatatta cgtactgcta agccatatgt
     8701 ttttctatga tcttaaaaat tactgggtgc gggagtacaa aacggctttg aatcagggaa
     8761 tttcactgag gaaatctctg atggaaatat atgaatatct gatggagcat ggggaatttc
     8821 cggagagctt ttatttatga aaggaatgga ggatacactt atatgtggct gtttttcgcg
     8881 ctgctgtccg ctctctttgc ggcccttact tccattttgg caaaacttgg aatcgatgga
     8941 gtaaattcaa atctggcaac cgccatccgg accgttgtag tcgtagtgat ggcctgggga
     9001 atggtatttc ttacccatgc tcaaaacgga attttttcga ttggaagaaa aagctggatt
     9061 tttctcatcc tgtccggcct tgccaccggc gcctcctggc tctgctacta ccgtgcatta
     9121 cagctgggag acgcctcaaa agttgttcct gtggataaac tcagcgttgt gatgacgctg
     9181 atccttgctt ttgtctttct ccatgaagat tttaccgcaa agtcgctcat agggtgtatt
     9241 cttattggag ccgggacgtt ggtaatggta ttataggtat actgctgaag ctttttacct
     9301 ctccagaaaa tctataagta aaaataaaat caaattggaa aggtgacgat tatgttaaag
     9361 aaaactcata tgctatacaa caaccggaaa tggatttcca acggtatgac agccagcata
     9421 aaccgcataa aggatatcct ggcgatcagt tttcagcctg tggtagggga attaaaccaa
     9481 aaatacaaag cgtctatcca ccctctataa gataaataga cgctctgttc atttgtatat
     9541 catgtttctt catactcaaa aatcagttct tctgtcaaat actcatcgct catacaatgt
     9601 aaatctgata ccccttcaca gagcaaagga taaagagcag aacaatagaa aaatttcagg
     9661 gcttcatcga aagacagatt tttctttttc gaaaatcgct caatgatcca ggcatatttt
     9721 tttgcagcaa aatcggattg gctgtcaaat ctgttcactc tcttcataat gcagatactc
     9781 atcaagaacc gtttgtgtac gcaggcacat ctgcaggttt agtttttcat aacgcaatcg
     9841 gtgaatcgct tccttcttat caattaaatt atcaaagtaa agttcaacag tattaaatac
     9901 tttatcattt gcagcaccgc caatcactat atcgtaatcg gttgcatcat tccccttctg
     9961 catttcagaa ttttaagtct ctgataagct gattcgtcaa aactgtagtg agaaacaatt
    10021 cctcttttcc cgcgccgctt aaatctttca caccacttta ttgcctgttc ataaagtggt
    10081 gttgtataaa aaccacgccc aaaatcaaga ttctctctgg aatgtactaa atccggagtt
    10141 tgaacttcca aatatgaacc atgatataaa atcataattg gatatccatt tcttctataa
    10201 gattaataat atcgtccaca atatattc
//
LOCUS       DBMDUH010000094        10192 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_3199_length_10192_cov_3.904965, whole genome shotgun sequence.
ACCESSION   DBMDUH010000094 DBMDUH010000000
VERSION     DBMDUH010000094.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 10192)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10192)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10192
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3199_length_10192_cov_3.904965"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="ACZPCG_08765"
     CDS             complement(<1..207)
                     /locus_tag="ACZPCG_08765"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HIR1285182.1"
                     /translation="MILKDIWLDIKAVQERDPAARSAFEVLLLYQGIHALIWHRFAHW
                     FYQHKMFFIARLISQIARFFTLIEI"
     gene            complement(436..855)
                     /locus_tag="ACZPCG_08770"
     CDS             complement(436..855)
                     /locus_tag="ACZPCG_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease complex subunit PrcB family protein"
                     /protein_id="HIR1285183.1"
                     /translation="MKRVLRILWIMGVIFSLAAGLAGCSVSKENRDKVRDLEFQTAPS
                     EELPQELSKLISEKVSQPFKLTYTDDKNLYIAVGYGAQPTGGYSISIRELYLTENSIV
                     LATELMGPEKGEDPGTEQSFPYIVLRTELLEEPVIFK"
     gene            complement(923..1948)
                     /locus_tag="ACZPCG_08775"
     CDS             complement(923..1948)
                     /locus_tag="ACZPCG_08775"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008724416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HIR1285184.1"
                     /translation="MEKKNLDWGNIGFSYMPADMRYVADYKNGAWGEGRLTADSNVVL
                     NECAGILQYCQECFEGLKAYTTEDGSIVTFRPDLNAERMMDSASRLEMPVFPKERFLE
                     ALDQVVKANAAWVPPYGSGATLYIRPYMFASGPVIGVKPSDEYQFRMFVTPVGPYFKG
                     GAKPITICVSDFDRAAPHGTGHVKAGLNYAMSLHAHETAHANGFDENMFLDPATRTYV
                     EETGGANFIFITKDGEVVTPKSDSILPSITRRSLMYVAEHYLGLKVTQRPVKLEELKD
                     FAECGLCGTAAVISPVGKINDHGREICFPSGMEEMGPVTKKLYDTLTGIQMGRIKAPE
                     GWIRKIM"
     gene            complement(2169..2393)
                     /locus_tag="ACZPCG_08780"
     CDS             complement(2169..2393)
                     /locus_tag="ACZPCG_08780"
                     /inference="COORDINATES: protein motif:HMM:NF013627.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285185.1"
                     /translation="MATGWFRDGDYLFYLHPYSDGTQGYMYTGWHQIDGKWYYFNEAS
                     DGTKGRLMTDTYIDGWYVDKDGVWDGKSQK"
     gene            complement(2637..4838)
                     /locus_tag="ACZPCG_08785"
                     /pseudo
     CDS             complement(2637..4838)
                     /locus_tag="ACZPCG_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009220038.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
     gene            complement(5589..7595)
                     /locus_tag="ACZPCG_08790"
                     /pseudo
     CDS             complement(5589..7595)
                     /locus_tag="ACZPCG_08790"
                     /inference="COORDINATES: protein motif:HMM:NF040438.5"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
     gene            8068..9819
                     /locus_tag="ACZPCG_08795"
     CDS             8068..9819
                     /locus_tag="ACZPCG_08795"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1285186.1"
                     /translation="MKLLISLLTIGLLPLVSISVFYQHFLDVRITKDIESASIDRLRY
                     TSYDIRRQMEISDQLLGWITYNRKLQEILTANYSEKYEKQLDIIQFSSYATEYAINAN
                     IENNIFKILIIEDNGESFQIGNVRSLMDQDAIKDARWAETYNHSKADQLVLSKDIYAK
                     DTYVFPVSSRIYNDLTGAPIGWCIIAFCNDMYSKNLVNGSNNQNIYLINPSGQCIGHT
                     DFSMIGADMSQDSMIREILNTQDSIGHITANRSHTPLIAHYYRIPDSNIIAVQETPLL
                     SFLSEKSEILRFSATLIVISAALVLCLTMYLHRILTRPINTISSYIKNVSKGDFTGSL
                     TLKNNDEFQTIADSINKMEREIRELMEAQKQESEIKKELEFRVLQNQINPHFLYNTLN
                     TIKWMASLQHADTIRDMTAALGRLLQNISKGSDKISIYEEMSLLDDYVLIQDIRYDGR
                     LNVRYHIGDPEITQALILKFLFQPIVENAIFHGIEPKGDGSGTIDIYLNQQDKSIYIH
                     IVDNGIGMTPEQIEQLLNPEESSQNRRGLNGIGISNINQRIKMTYGQEYGISISSKPG
                     AYTDVLICIPYERSTSL"
     gene            9816..>10192
                     /locus_tag="ACZPCG_08800"
     CDS             9816..>10192
                     /locus_tag="ACZPCG_08800"
                     /inference="COORDINATES: protein motif:HMM:NF012301.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR1285187.1"
                     /translation="MTSILIVDDEALIRRGLESLIPWERLNCSLADQASNGAEGLEKI
                     RRYHPDIVFTDIKMPQMDGLQMIEKALLEPDPPLFIILSAYNDFELVRSAMRLGAIDY
                     LIKLNLEENELVSLIQSAQKMLS"
BASE COUNT         2607 a         2634 c         1879 g         3072 t
ORIGIN      
        1 gatctcgatc agcgtaaaga aacgggctat ctgtgaaata agcctggcaa taaaaaacat
       61 tttatgctgg taaaaccagt gggcaaaccg atgccagatc agagcatgta tgccctgata
      121 cagcaaaaga acctcaaacg cactgcgggc cgccggatcc cgctcctgca cagcctttat
      181 atccagccaa atatccttta agatcatttc tgctaccaac ctttcccaaa tgcgtccatt
      241 ttgattcata acgcattttg atgtagtata gcatatgatc ccggcgaaaa aaaggatttt
      301 cttgcgtttc cgcacgttac agtgcacgct ggcatctgga gttcaggacg actttgaagg
      361 ccattagaaa accggagtcc tgaacacaga cgccagaatg cagtgcagcg tgcggaaacc
      421 cgccttttct ttctactact taaaaataac cggctcctct aaaagctctg tccgcagtac
      481 aatgtagggg aaggactgct ccgtgcccgg atcttccccc ttttccggcc ccatcagctc
      541 cgtcgccaga acaatggaat tttccgtcag ataaagttct cttatgctga tactgtaccc
      601 ccctgtcggc tgagccccat atcccactgc tatgtacagg ttcttatcgt ccgtataggt
      661 cagcttaaat ggctgggaca ctttctctga aatcagcttt gaaagctctt ggggcagttc
      721 ctctgagggg gcagtctgaa attccagatc ccgcacctta tccctgtttt cctttgaaac
      781 ggaacagcct gcaagccctg cggcaagaga aaaaatcact cccattatcc aaaggatcct
      841 taaaactcgt ttcatagctg caaaaacctc ccggaatata ccatctgtta tcagtatatt
      901 ccgggaggcc tgtctgagac ccttacatta ttttacgaat ccagccctca ggcgccttaa
      961 tgcgtcccat ctggataccg gtcagcgtat catacagctt ctttgtaact gggcccatct
     1021 cctccatacc gcttgggaag cagatttccc tgccgtggtc atttatcttt cctacaggtg
     1081 agatcacagc cgctgtaccg cagagaccac attctgcaaa atcctttaat tcctccagct
     1141 ttacaggcct ctgtgttacc tttaagccca gataatgctc tgccacatac atgagagaac
     1201 gccttgtaat agatgggaga atggagtcgg acttgggcgt aacaacctct ccgtcctttg
     1261 taataaagat gaaattggct ccgccggttt cctccacata cgttctggtg gccggatcca
     1321 ggaacatatt ttcatcaaat ccattggcat gagccgtttc atgggcatga aggctcatag
     1381 cgtaatttaa tccagccttt acatgaccgg ttccatgagg agccgcacgg tcaaaatcgc
     1441 tgacgcagat cgtgatcggc ttcgctccgc ccttaaaata agggcccaca ggcgtcacaa
     1501 acattctgaa ctgatattca tctgatggct taacgccaat caccgggccg gaagcaaaca
     1561 tatacgggcg gatataaagc gtcgctccgc tgccataagg cggaacccag gctgcatttg
     1621 cctttaccac ctgatccaga gcctctaaaa aacgctcctt tggaaatacc ggcatttcca
     1681 gacggctggc agaatccatc atacgctctg catttaagtc cggacggaag gtaacgatgc
     1741 tgccgtcctc tgtggtatat gcctttaatc cctcaaagca ctcctggcag tactgcagga
     1801 tacctgcaca ttcattgagc actacattag agtccgcagt cagtctgcct tctccccatg
     1861 ctccattctt ataatctgcc acatatcgca tatctgcggg catatagcta aaaccgatat
     1921 tgccccaatc cagattcttc ttttccatct ttatttcctc cattcctgtc cgcggaatta
     1981 ttttacagcc attatagact tattgaatta gaaaatcaac ggaaaaacgg acagccgggt
     2041 atatcattcg gatatgggag gtataactcc tgtcaaaatg agggaatcct caaagccacc
     2101 atctggcccg aagattccct ctcagctgtc tcacctgtta tgcagtatta cacttcaggt
     2161 attcatattt atttctggct ttttccgtcc catacgccat ctttgtccac ataccatcca
     2221 tctatatagg tatctgtcat taaacggccc ttggttccat ctgatgcttc gttaaagtag
     2281 taccactttc cgtcgatctg atgccagccg gtgtacatat atccctgtgt tccgtcagaa
     2341 tacgggtgaa gatagaacag ataatctcca tcacggaacc aaccagtagc catgtaacca
     2401 tcttccttaa aatagtacca actgattaca ttattccaaa ccagctgttt ccattctcca
     2461 gcaggccatg ttccatcagg atactcaaac caccagcctt tatcgtctct gtgccatgtt
     2521 cctgcggtat tttctgcaga agtatacact cttccgccgg aagctgaacc ggaactggaa
     2581 ccatcagagc ttcctccatg atttccattg ctgtttccat cgtcacttcc gccataggaa
     2641 agaccaaatc ccaattcata ataattgccg tcactgtctc tgtatgcgta tgcctttcca
     2701 ttctcatcct tcatagattc cggcatatac tgatcctttg catatccggg aacatcgtta
     2761 ggagaaatgc aaacgccgtc tgcatttact tcaattacac tatcatttct cggaagagta
     2821 atcggcatct ttccggtcgg ttcatatgca cctgtaataa catccatagt tgcagatgta
     2881 agtgtatcaa aaccggcaag gacaacatct gaatatggct ctacattgcc aagcatccag
     2941 gcaagcgtaa agtttacatt tgtaataacc tttcctccat ttgctcttac agcttctgaa
     3001 atcgcctgaa tttcctgagc attttcaatc gtagattcct catgcgttgc ttcaatcgga
     3061 cgtccctgct catctacatc atggactgtc ttattcgtac atatatccag ttcaagatat
     3121 cccttcgttg ctgaaaaata atttccagaa gacgggttta caaaaagcag tgcataatct
     3181 gcatcattat aattattggt taatgtaaga ttatatccgc ctgccgcaag gcttttgcga
     3241 agctcagagg ttgtttcggc agacttttcc tgtccaaagt actgaataaa tacctttttg
     3301 ccctcaagct tatcttcagt cagaggcaga actccttcat tgtccttcaa aagaacaact
     3361 gacttctgat gtgcctcata tgcctcatcc caattgcttt cattatctac aactgccttt
     3421 gcttcctcag gatcgcggta cggattctca aacagaccca actcaaacat ttcttttaag
     3481 agacgctcgt tggactgatc cagatcagcc gttgtcagcg tcacatcatc taccgtataa
     3541 ccatccggta ccggtttctc tgtctgatag taatcagatt taggtccgtc aggtcctctg
     3601 ttccatgctt ctacaattga gttgacatcg ttcgtatcac tgataatgtc tgttccggca
     3661 ttaacagcaa gtgcatttct ttccggaaca tccagttctt ctacacccca tgccatattt
     3721 ccggtaattc cgctgtcgct gtttacatat ccttcatgac caatctgatc tcttaaaaga
     3781 ccattgataa ataccttatt aaacgcaaat ccaaccggaa ccatctcaat tggatcaccg
     3841 tttaaatcat actgcggttc actcttctga gctgcaggtt ttgcataata cggcataatt
     3901 gatgaaacat tattttctac tgctttgata aaggctggga gatgatattt ttccaggctt
     3961 ccctctgtct ggtatacatt ccactggccc tgtgaataat gcggatcaaa tccgttttct
     4021 ctggcgcctc cgcccgggaa gtgctttaca gtaaggccaa ctccgttttc ttccacacct
     4081 tccgttccgc cctggaagcc ttctaccagc cgtccaataa tatcgctgat cagttccgga
     4141 ctctcaccaa aggttccata cgttctctgc catcttgggt ctgtcatcgt atctgccata
     4201 tacatatagc cctttttcag accgcaggca tcccactcgc ttctggcaat ctctgcaaaa
     4261 cggctgatca ggcctgcatc tccgccattg gcaatatcac caattacagc agcagccagt
     4321 ccgagcgttc ccggccatgt agagaataca ccggacgcat cattcattcc aaaggtcatc
     4381 tgtccgtttt catttctgga gttggaagcc ataacaacag gaatacctaa acctgttttt
     4441 tctgccacca gattcatctg gttcatccag gaagccatat cttccggttc cggattctga
     4501 cgaagaataa agtgacgcag tccccaatct acaatagcct ctgttgttcc cagtgtgcgt
     4561 gatacgccga aaatttcaga agacatatcg tctgtatttg aggtttcatc taaaagccct
     4621 gtttctttat caggatcctt actgtagata cccataccgc gggaattgat aaccatcatt
     4681 ccggcctttt ccctgattgt catcttttct accagatcct tcgcacgtgc ttccggtgtt
     4741 tcgcgccagt cctcatatac atccagctgt ccgtttccat tggaatcctt gaagtaggaa
     4801 ccgtccgatt ctatgatact cttcacagta gctccgattg taggtccatt atcattttcc
     4861 ttatatacaa ggctgtcata ccatgcggat actgtattgt ccgttgcggt cggataagtg
     4921 gaatccaggc tgtttaactt catcagttta ttcggttctg ttaattcgta aattccatct
     4981 acgccggctc cgctgatctt gtatccatta aaatgattaa atacattgat ataggtatat
     5041 gcacatgcat tatcagcctt cttaattccc agatacagct tggatggtct ttctcccaga
     5101 agatcttccg ctgcttctgc tgtctggctt atgatatccc aaagaaggcc atcctcttct
     5161 ctggttggat ccttctcttt atccaatact ttccagctgc atcctttctt ttcagcagcc
     5221 tttgcatctg cggtgatacc aatatacgca attcccttag ccttcagttt ggttacatca
     5281 aattctgaat cgttctcaaa catcggcccg attactgcct gacccttata gtcatccgga
     5341 atgcatacgc ggatatagtt ggagtatccc tgaagcggct ttctgatata aggaatatcc
     5401 tccagaagaa cgccataaat attggccttt ccttctgccc gatatgtaat ctccgtatac
     5461 ccgtctttct tttcttcctt aagcttcttc agaatcggct ttgtctgcat ggaggtttca
     5521 atgtctttaa accacgccgg acgatgtccc aaaacaggct ggttttcagc gatggacatc
     5581 atacgatccc gctcttcttc aatcagatat ccttctgcaa tcgcctcatc cagagaactt
     5641 tcatactgac ttctgtagtc ctcataatca gtatataatt cctttaattc ctcctgagac
     5701 agatatacca ttgatccgcc gtctgtagaa gtttttccat taacgcctcc gtaatatttg
     5761 gcaaccggaa ccgaaatctg aggtggtaca ataccttctg tcagatttcc gtatgcatcc
     5821 ctggaatcca ggttaacatc ctctaccgta aaaccttccg gcatatctga gccatctacc
     5881 cactcaatca gatttgcaaa gacagaattc acataagaat ccatattcag gtctgtattc
     5941 gcattcggac ggtctgtctc cgcatccatc ttcatcggcg gataattgcc ggcgggacgt
     6001 cctgcctgaa tctgtatctc atccgtcata tctggtggga aaatctttga agaatgggcg
     6061 ccgcctgcta ccacataatg acgatacata tctacgcccg gttcatcccc atcctctttt
     6121 atgtaatccg gatttgactt aacacaactg gaataatcat tttctccctc taacagaatg
     6181 tagggtacag gagaatcata aggagtcggg aaacttctgg atgcgccgtg actgcttaaa
     6241 tctgtatctg cgtatgcgcc cactacattg acaaaaccat caaaaaccgg atttccgtca
     6301 cccgtatctt tatcataatc cataccaact gcattggcct gaccaaagca gttcacatag
     6361 gtgttcacat aaaaaccgga ttgagactgt cccataagga atactttgtc tgctgtctct
     6421 ccgcctaaaa gttcactatc cggagttttc agcattacgc ccaactgtcc cagcatatcc
     6481 cagaacaatc cgttgcggtc tttttcattc agtcccttat ctccccattt aaggttttca
     6541 taccgtttgg catcaaaacg ttttaaggat tctacattgc agtactttga agtaatgccc
     6601 acataggcat atccatttct cataaagtag tcatagcttc tgcgccagat atccggcaga
     6661 tccaccttgc tgctggcatt cagaatatct acaaatacaa ctccgttaaa atcggcagga
     6721 tcttccggca tatacacaaa tacacgattg gtatagtcct gcttgtccgg tctagacgca
     6781 ttgcttctgg atgcattgct tccggttgcg gttgtaacct tatggtcgtc ctttaaatcg
     6841 tatacccgtg tatttccgct gaggaaatat tctgtttctt tgtagttgta tttatctgtg
     6901 aaaccaatat tctgcttcgt tcttccgtct ttctggctca gatacataaa actgtcagaa
     6961 gtttcaggaa tttccttaac cgacggagta tcgttatgca tctcaactac cagatccagc
     7021 atatccttgc tgagagattc attgcgaagc gcattactgt ccgttgcttt tttaggttca
     7081 ctgggcgtag ccaatgtaat ctggctggga gtcgctactc tctctggaat ctgtacggca
     7141 gaagcggtct ctttttcttc ctcaatttct gctttttctg attcaggctc ttcttccgtt
     7201 tctgcttctt ctgtctcagc ttcctcctca gtttctgcct cttccgactc agattcttct
     7261 tctgtttctt cttccttcga ctcggctccc tcctcggttt ctgcttccga ttcagactct
     7321 tcttccgttt ctgcttcttc tgactcagct tcctcctcgg tttctacttc ttctggctga
     7381 tctgaaccgt tttcttctag ggaatcctct ttttcatctt ccagatcgga acgttcatcc
     7441 cccgcttcat catctgttaa ttctgcacca taaacttcgc tgacatttgt catgccccca
     7501 cccccgggta taacgccaac tccggcggaa agactggagc cggtcataga agcagccagg
     7561 accgccgcca tgatcggttt cattttcttt ttcatgtttt ttctcctttc cttattctcc
     7621 cctattttct gccatgcgcg ctcaggcaga aaacattttg atttttaaca aaatgttaac
     7681 atctattaaa attatataaa ggtttgtttt ttattaaaat ggtatttttt taatatttat
     7741 agtataattt tatcttattt gtgcaatttg ttttcttttt tcttttattt tctttctata
     7801 tatgtacact ttatctattt ttgtgtattt caaaaaaata aattatagat ttatgttata
     7861 acttttcccc tttatccctt tgtttttaaa gcattcattc tattccatca aatttacttc
     7921 ccacatttgg attatttttc ttcatttttt gacactcttc ccaaattatg gtacacttat
     7981 tttgtataat tatgctgtaa atcagtacta ttactcacat tggagcgaca catgaaacat
     8041 tttccccttt ctgttcacag ttttaaaatg aaacttctga tttctctgct taccattggt
     8101 cttcttcctc tggtttccat atctgttttt tatcagcatt tccttgatgt acggatcacc
     8161 aaggacatcg aatcagccag tattgaccgg ctccgctata ccagctacga tatcagacgg
     8221 cagatggaaa tctccgacca gctcctcgga tggatcactt ataacagaaa gctgcaggaa
     8281 atactcactg ctaactactc tgaaaaatat gaaaagcagc tggatatcat ccagttcagt
     8341 tcttatgcca cggaatatgc aatcaatgca aatatcgaaa acaatatttt taaaattctt
     8401 atcatagaag ataatgggga atcttttcag atcggaaatg tccgttccct tatggatcag
     8461 gacgcgatca aagatgccag atgggcggaa acctataatc acagcaaagc agaccagctg
     8521 gttctcagca aagatattta tgcgaaggat acctatgttt ttccagtcag cagccgtatc
     8581 tacaatgatc tgaccggtgc tcccatcggc tggtgtatca ttgccttttg caatgatatg
     8641 tattccaaaa atctggtaaa cggctctaat aatcagaata tctacctgat caatccgtcc
     8701 ggccagtgca tcggccatac ggatttctcc atgatcggcg ctgatatgtc tcaggattct
     8761 atgatccgtg agattttaaa cacccaggac tccatcgggc atattactgc aaaccgcagc
     8821 cacacgcctt taatcgccca ctattaccga ataccagaca gcaatatcat tgcagttcag
     8881 gaaactcccc ttctctcttt tctcagcgaa aaatctgaga ttttacgatt ctctgccaca
     8941 ttaatcgtaa tttccgctgc gctggtactc tgccttacca tgtacctgca ccgcatactg
     9001 acccgcccga tcaataccat cagctcctat attaaaaatg tttcaaaggg cgattttacg
     9061 ggctcactga ctctgaaaaa taatgatgaa tttcagacca ttgcagactc catcaacaaa
     9121 atggagcgcg aaatcaggga actgatggag gctcaaaaac aagaatctga aataaaaaaa
     9181 gagcttgaat tccgcgtcct tcaaaaccag atcaatccgc attttcttta caatacctta
     9241 aatacgatca aatggatggc ttccctgcag cacgcagata ccatccggga tatgacggca
     9301 gctcttgggc gtcttctgca gaatatttcc aagggttctg ataaaatttc tatctatgaa
     9361 gaaatgtccc tcctggatga ttatgtactg atccaggata tccggtacga cggacggtta
     9421 aatgtccggt atcatatcgg agatccggaa atcacacagg ccctgatttt aaaatttctg
     9481 ttccagccta ttgtagaaaa tgctattttc catgggattg aaccaaaagg cgacggctcc
     9541 ggaaccattg atatttactt aaaccagcaa gataaaagta tctatatcca cattgtcgat
     9601 aacggtatcg gcatgacccc ggaacagatc gaacaacttt taaatcctga ggaaagctcg
     9661 cagaaccgcc gcggattaaa cggaatcggc atcagcaata ttaaccagcg gatcaaaatg
     9721 acctatggac aggagtatgg tatttccatc agcagcaaac ccggagccta tactgacgtt
     9781 ctgatctgca ttccatatga aaggagcact tctttatgac ctcaatctta atcgtggacg
     9841 atgaggcttt aatccgccgc ggtcttgaaa gcctgattcc atgggagcga ttaaactgca
     9901 gtcttgcaga ccaggcctct aatggagcag agggactgga gaaaatccgc cggtaccacc
     9961 cggatattgt atttactgat attaaaatgc cgcagatgga cggactgcag atgattgaaa
    10021 aagctctttt ggaaccggat cctcctcttt tcattatcct cagtgcctat aatgattttg
    10081 aactggtccg ctctgccatg cgcttaggcg ccattgacta tctgatcaag ctgaatctgg
    10141 aggaaaatga actcgtttcc ctgattcagt ctgctcagaa aatgctttca aa
//
LOCUS       DBMDUH010000095        10089 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_3230_length_10089_cov_4.633260, whole genome shotgun sequence.
ACCESSION   DBMDUH010000095 DBMDUH010000000
VERSION     DBMDUH010000095.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 10089)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10089)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10089
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3230_length_10089_cov_4.633260"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(111..611)
                     /locus_tag="ACZPCG_08805"
     CDS             complement(111..611)
                     /locus_tag="ACZPCG_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285188.1"
                     /translation="MKRLTQKDEQGNWCLKGVSWEQLHEGQVITKSLRERLYGALWKL
                     MEYEDTGLTPEEIERLKEQHRWIPVEERPPVQGEPVWATIKHSEWINDYDTDWLPEEE
                     KKYHPESYGVYKAEYIGGGIWQYSDDYNEWIYCDAVEKEERNLANVYDTVTAWMPLPE
                     PYQPEE"
     gene            complement(586..741)
                     /locus_tag="ACZPCG_08810"
     CDS             complement(586..741)
                     /locus_tag="ACZPCG_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285189.1"
                     /translation="MAKIWMQGKERFMCRVCENEEIELGQKYCQICGEALEWEEEGEN
                     EEINTER"
     gene            complement(744..2402)
                     /locus_tag="ACZPCG_08815"
     CDS             complement(744..2402)
                     /locus_tag="ACZPCG_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285190.1"
                     /translation="MIEDLRERGRQKENENTMVPETGIIDHTGPEWYQMEIPLDEIGR
                     YIHTDLKTAARSVISIGYWLMYVRDRNQFQEYGYSNINEYAARQFGFSRSTTKRYIDR
                     CIRFSKGGNSPILDDRYRDFSKAQLQEMLGLDDEQLEQVTPNMTVRQIRAMKQPKEIP
                     YVELPGQISITDFPGIEPEAAPDVTDVPPEPERAAMQSCEISVEDLMPEVEPLPVESV
                     ATSQRENCPAHQLSAYGTPKREYPADSLIATEGCEGGHYCDSCSMECGIRQKDRWCVE
                     APCGNPFPCEIRDLDGIRELAGDTCEFVNHDLAYYRAGDGEPSPCCKHCKNPCEYICG
                     RAMRALDQGLEQPAVEMQQNEEICCENDSVDEVATLQPEEAAEPTDTELLREMLGKEK
                     KYLEEMIKVDAVKGFPKKLLRKKKILVAALAGMMCDLDMPEPEEPEQPELPIMKNNDQ
                     RKAWLRDYKSWGLWYTDEHIGARYYKYDFENGARLIVEVYTQHNEFTGQDYESSYLHL
                     VGGPEPEKHPTGAYGRWQRHETYSRYPDSETELVEFLKALQKGK"
     gene            complement(2386..2646)
                     /locus_tag="ACZPCG_08820"
     CDS             complement(2386..2646)
                     /locus_tag="ACZPCG_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285191.1"
                     /translation="MEEAQAAVLARYRVDQGAGGYYAKMMDKDEVIARRAYMRNILRV
                     SFLWCTMNNSQLDNMRLYRMGEDFIVEDTDNRDFVLKIDRRP"
     gene            complement(2609..3187)
                     /locus_tag="ACZPCG_08825"
     CDS             complement(2609..3187)
                     /locus_tag="ACZPCG_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285192.1"
                     /translation="MNKREFSVLAATMEEYYGKNQITKSNTSMDIWFSLIGDIPFEDC
                     KNAVTQLMATSNFFPSAAEIRKLCSQMAYPEVPSIDDAWGMVLKAVRRYGYMQESKAL
                     ESLPEPCRTVVRNIGWQNICRSENIMTERAFFRDSYGPKIQEIHRQEALPLGIKKENR
                     ERIAAQVEKTAGLLMLGGGEDGGSTGGSAGAL"
     gene            complement(3180..4082)
                     /locus_tag="ACZPCG_08830"
     CDS             complement(3180..4082)
                     /locus_tag="ACZPCG_08830"
                     /inference="COORDINATES: protein motif:HMM:NF025655.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4373 domain-containing protein"
                     /protein_id="HIR1285193.1"
                     /translation="MARQQKTGLDYFPLDVNIFTDNKIRILKARYGVNGVSVYIYLLC
                     EIYKNGYYMQWNDDLKFILADELNLTDGFIEQVLKFLLERSLFDNKLFQSDTILTSPG
                     IQRRYQLAVKERAKKTPIRIKGFWLLEAEETEPFIKVNPDSDFSENLADSSRKNKDSS
                     QKNDIKESKVKKSKEKENKENRTAAAAPVFSPQSFEMLCVNTLIHSCLDNFPGAKVPV
                     TDEEKNQWCVHIERMKRVDRRTEEEIRTALEYAVENPFWKANIRSTRKFREKFETLYI
                     QARSNRTAAETSKEERLRRWADNE"
     gene            complement(4054..4434)
                     /locus_tag="ACZPCG_08835"
     CDS             complement(4054..4434)
                     /locus_tag="ACZPCG_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006566308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285194.1"
                     /translation="MGKSQREKGKRGERELAGLLREYGYDCRRGQQYCGASGDADVVG
                     LLGIHIECKRVEKLNLLDAVSQAVRDAAKGLLPAVFHRKDRCEWLVTMRFVDWMELYK
                     EAEAGKALKEKGRAGNGETAEDRS"
     gene            complement(4550..5302)
                     /locus_tag="ACZPCG_08840"
     CDS             complement(4550..5302)
                     /locus_tag="ACZPCG_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003503010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285195.1"
                     /translation="MLEGLKAALEHVEGLARENEKTEVIEICGHTYANKSLKRYDNTD
                     KATALEASSLTALLGYIGACHEEFPKDTDMIIHIVNPKRVRLITALDAERTRECLFEV
                     NAEVNEFRFGHWYDQEAFMIELQANFQPSEDLELVQKMAGNVERKNDQSYADDGISQV
                     AVMTVGAAAKANVKVPNPVTLVPYRTFQEVGQPASAFVFRVGDKGEPTFMLQEAQNGL
                     WRNEAVANIKAYLAQAVSHMQPNVRDHITIIG"
     gene            complement(5316..5474)
                     /locus_tag="ACZPCG_08845"
     CDS             complement(5316..5474)
                     /locus_tag="ACZPCG_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285196.1"
                     /translation="MASEKKCCGTCRYHLHDDDVDFICDCEESEGYGDYTMYQDCCDC
                     YEARNIEE"
     gene            complement(5485..5916)
                     /locus_tag="ACZPCG_08850"
     CDS             complement(5485..5916)
                     /locus_tag="ACZPCG_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285197.1"
                     /translation="MAITFDTIANGELAEKFQMALTQIGRNIMDPNTDPEAVRGMTVN
                     IKFKPMGRGNLKVDFSVGTKLAGFKKSETVFLVGQDGRTGRISMSEPGSGLPQVTAIR
                     ETPAAAYTDVRQEPEFDPETGEIFEEPVQAGSIDLRQAYGR"
     gene            complement(5939..6700)
                     /gene="bet"
                     /locus_tag="ACZPCG_08855"
     CDS             complement(5939..6700)
                     /gene="bet"
                     /locus_tag="ACZPCG_08855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HIR1285198.1"
                     /translation="MAVNNSLAKKPVQKVEVTKYMANGMEVTLTPGTVKNYLVSGDKE
                     RVSDQEVAMFINLCRFTGLNPWLREAYCIKYGNEPATLVVGKDAYFKRAESNENYDGM
                     EAGIIVQQEETEEIIYRQGTLKLDGEKLVGGYAEVFRKDRGHSFRIEVAFDEYAGRKK
                     DGSLNSQWSKKPATMIRKVAVVQALREAFPKTFAGMYVAEEVGAVDPDYTAGDIIEPQ
                     AQRAIEQSEPVQPSASEPVSQTAPQPQDASSAFFG"
     gene            complement(6716..7597)
                     /locus_tag="ACZPCG_08860"
     CDS             complement(6716..7597)
                     /locus_tag="ACZPCG_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003503007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1351 domain-containing protein"
                     /protein_id="HIR1285199.1"
                     /translation="MELKIYNPQEGGYLKKIEWNFEELKEEVTAKAKEYAATVYTDET
                     IKAAKADRATLNKFVEAVKGERTAIRKKLLEPDELFGEQIKELTGIVQSAIDSIDVQI
                     EDYESRQRKEKTAKIKEFYEANIHDIGQYLPFDRVMKPQYANASTTIKSIKEEILALI
                     QKVDEGLAILNEVDSPYVGDMKQVFLQTYDIGAAMARRNQLEAEAENRRLYMEEQERR
                     KAEREATRKAEADRVMQAGRAPEPEPPKETPQKIETVEEPVNVIDFRVYATMAQLMRL
                     KTFLNDNGIRFEPVPKN"
     gene            complement(7615..8268)
                     /locus_tag="ACZPCG_08865"
     CDS             complement(7615..8268)
                     /locus_tag="ACZPCG_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003503005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda-exonuclease family protein"
                     /protein_id="HIR1285200.1"
                     /translation="MAVTKHIFASREEWLKGRKNHIGGSDASACVGMNPYKDNVQLWE
                     EKMGLVMPEDISDKDYVRYGTEAEKHLRALFAMDFPEYQVFYDEDNMFINDRYPWMHA
                     SLDGELVDEQGRRGILEIKTTNILQSMQLEKWKGKIPDNYYCQVLHYLAVTEYDFAVL
                     KAQLKSEWHGELQITVKHYFIERKEVEEDIRYLVEAEQRFWACVVEGRRPNFALPVI"
     gene            complement(8422..8721)
                     /locus_tag="ACZPCG_08870"
     CDS             complement(8422..8721)
                     /locus_tag="ACZPCG_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285201.1"
                     /translation="MVNTTILYEGIKKTVESEIAFAICLTGNKEKNGVDTTVICIGER
                     ENISSDDLTKCLAKSMVDAVSSMAVDDLGAVHVLTELARIVGEAAKEKCLERLSK"
     gene            complement(8911..9105)
                     /locus_tag="ACZPCG_08875"
     CDS             complement(8911..9105)
                     /locus_tag="ACZPCG_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285202.1"
                     /translation="MKKATTVAEYKIRKCLAKLERQGDIALECFAFEMIGPREAELRD
                     GNGDAFRLAYDPVSESVSEI"
     gene            complement(9122..9346)
                     /locus_tag="ACZPCG_08880"
     CDS             complement(9122..9346)
                     /locus_tag="ACZPCG_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285203.1"
                     /translation="MQSEVIKYQPPVEGVVVTQEEKQQILKLGRIMREQLDQSHKDVR
                     LFGRMSTVMAFLLGVESAVLFLMATGIIAL"
     gene            complement(9327..9665)
                     /locus_tag="ACZPCG_08885"
     CDS             complement(9327..9665)
                     /locus_tag="ACZPCG_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285204.1"
                     /translation="MTESVIQLTQAKEIGEHIARMLMSENSVPEFGEKGVPVAVAARV
                     FGKDATWIQAGIISGWLPIGIATQDKRQVTDLKQMESGKRTNYYISPKKLWEWTGYIW
                     RGEENAIRSN"
     gene            complement(9670..9870)
                     /locus_tag="ACZPCG_08890"
     CDS             complement(9670..9870)
                     /locus_tag="ACZPCG_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005427169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1285205.1"
                     /translation="MYEKYVTLRDKRNVTDYRVSIDTGITKSTFSDWKSGRSNPKLDK
                     LLILAKYFDVPVEYFAETEDGR"
BASE COUNT         2141 a         2996 c         1947 g         3005 t
ORIGIN      
        1 tccgggccgg aaaagtaagg aggatcagtg atagcaatgt caaaatattt gtctgggaat
       61 tgtttcatgc cctccatgca gtccatgttg taatatccat attccataag tcactcctct
      121 ggttggtacg gctccggcag tggcatccag gcggtcactg tgtcatacac atttgccaaa
      181 ttcctttcct ctttctccac tgcatcacag tatatccatt cgttataatc atccgaatac
      241 tgccagatcc ctccgcctat atattccgcc ttatacacac cgtagctttc tggatgatat
      301 ttcttctctt cttccggaag ccaatcagtg tcataatcgt tgatccattc agagtgtttt
      361 atagtcgccc aaactggttc tccctgcacc ggcggcctct cctccacggg aatccagcgg
      421 tgctgctcct taagccgttc gatttcttcc ggtgtcagcc cggtatcctc gtactccatc
      481 agcttccaga gagctccata cagccgctcc cgtaaagact tggtgattac ctgtccttca
      541 tgcagctgtt cccacgacac acccttcaag caccaatttc cctgctcatc tttctgtgtt
      601 aatctcttca ttctcgcctt cctcctccca ctcaagtgcc tcgccgcata tctggcagta
      661 tttctgcccc agctcaatct cctcattctc acatacccgg cacataaatc tttctttgcc
      721 ctgcatccat atcttagcca tatctatttt cctttctgca atgccttcaa aaactccacc
      781 agttctgtct cactgtctgg ataccggctg tatgtctcat gtcgctgcca tcttccgtaa
      841 gccccagtcg gatgcttctc cggctccggc cctccaacca agtgtaagta tgaggattca
      901 taatcctgcc cagtaaactc attatgctgg gtgtatactt ccacaatcag ccttgcccca
      961 ttctcaaaat catacttgta ataccgggcc ccgatatgct catctgtata ccacaggccc
     1021 catgatttat aatctctcag ccatgccttg cgctgatcgt tgtttttcat aatcggaagc
     1081 tccggctgtt ccggttcctc cggctctggc atatccagat cacacatcat tcctgcaagg
     1141 gctgccacca gtattttctt cttccggagc aactttttcg ggaatccttt cactgcatca
     1201 acctttatca tttcctccag atacttcttc tcctttccta acatctctcg gagtagttcc
     1261 gtatctgtag gctcagcggc ctcctccggt tgcaacgtcg caacttcatc cacactgtca
     1321 ttttcgcagc agatttcctc attctgctgc atttctacag caggctgttc cagcccctga
     1381 tccagtgccc tcatagccct accgcaaata tattcgcaag gattcttgca gtgcttacag
     1441 catgggcttg gctccccgtc tccggcccgg taataagcca gatcgtggtt cacgaactca
     1501 caagtatctc ctgcaagttc acggattcca tccagatctc taatctcgca cggaaacgga
     1561 ttcccgcagg gtgcctctac acaccaccgg tctttttgcc ggatcccaca ctccatagag
     1621 caggaatcac aataatggcc tccctcacat ccttctgtgg cgatcaggct gtctgctggg
     1681 tattcccgct tgggtgtccc ataggccgat agctgatggg ctggacagtt ttcccgttgc
     1741 gacgtcgcaa cgctctccac cggcagaggc tccacttcag gcatcaagtc ctccaccgat
     1801 atctcacagc tctgcatagc tgctctttct ggttccggcg gtacatccgt cacatctgga
     1861 gcggcctccg gctcaatccc cggaaagtct gtaatactga tctgccccgg aagctccaca
     1921 tatgggattt ccttaggctg tttcatggct cggatctggc gcacggtcat atttggagtt
     1981 acctgctcca actgctcatc atcaagccca agcatctcct gaagctgtgc tttgctgaaa
     2041 tccctgtacc gatcatccag tatggggctg ttgcctccct tggaaaaccg gatgcaccgg
     2101 tctatatagc gtttggtggt tgaccggctg aaaccgaact gcctggcagc atactcattg
     2161 atattgctgt atccgtattc ctgaaactga ttacggtccc gcacatacat cagccagtat
     2221 ccgatggata tcacacttct ggcggccgtc ttaaggtctg tatgtatgta ccgcccgatc
     2281 tcatccagtg ggatctccat ctggtaccac tccgggcctg tatggtcaat gattcctgtc
     2341 tcaggaacca tggtattttc gttttccttc tgtctccccc tctctctaag gtcttctatc
     2401 aatcttaagg acgaaatccc ggttgtccgt gtcctccaca ataaaatctt cacccatgcg
     2461 gtacaagcgc atattatcca gctgggaatt gttcatcgta caccaaagaa aactcactct
     2521 caggatattc cgcatatacg ctcttctggc aatgacctca tccttgtcca tcatttttgc
     2581 ataatagcct ccggctccct gatctactct ataacgcgcc agcactgccg cctgtgcttc
     2641 ctccatcctc tccaccacct aacatcagca gtccagctgt cttctctacc tgcgctgcaa
     2701 tccgttccct gttttctttt ttgatcccca gcggaagtgc ttcctgtctg tggatttcct
     2761 gtattttagg cccgtaggaa tcacggaaga acgccctttc tgtcataata ttttcactcc
     2821 ggcagatatt ctgccaccca atattccgga ccactgtccg gcacggttcc ggaaggcttt
     2881 caagggcttt cgattcctgc atatacccat aacgtctcac cgctttcaaa accattcccc
     2941 aggcatcatc tatgcttggg acttccggat atgccatctg tgaacatagt tttcggatct
     3001 ctgccgccga agggaaaaag ttactggttg ccattaactg tgtgaccgca tttttacagt
     3061 cttcaaacgg gatatcccct ataaggctga accagatgtc catggatgta ttacttttgg
     3121 taatctggtt ttttccataa tactcttcca tggttgctgc cagcacggaa aattctctct
     3181 tattcattgt ccgcccacct cctgagcctt tcctctttgc ttgtttctgc cgctgtcctg
     3241 ttgcttctgg cctgtatata tagggtttca aacttttccc tgaactttct ggtactgcgt
     3301 atattggctt tccagaacgg attctcaacc gcatattcca atgccgtccg gatctcttcc
     3361 tccgtcctgc ggtctacccg tttcatgcgt tctatatgaa cacaccactg gttcttttct
     3421 tcatcggtaa ccggaacctt tgcacccgga aaattatcca ggcacgaatg gatcagagta
     3481 ttcacacaca gcatctcaaa tgactgtggt gaaaacactg gtgcggcagc agcagttcta
     3541 ttttctttat tttccttttc tttacttttc tttactttac tttcctttat gtcatttttc
     3601 tgggaactat ctttattttt ccgggaagaa tccgctaaat tctcggaaaa atcagaatca
     3661 gggttcactt taataaaggg ttccgtttct tctgcttcta aaagccagaa accctttatc
     3721 cggatcggag ttttcttcgc ccgctctttt actgcgagct ggtatctcct ctgtattccg
     3781 ggtgaggtga ggatagtgtc cgactgaaaa agtttgttat caaacagtga ccgttccagt
     3841 aagaatttca acacctgctc tatgaaccca tctgtgagat tcagttcatc ggccaggatg
     3901 aatttaagat catcattcca ctgcatataa tagccgttct tgtagatctc acagagcaaa
     3961 tagatatata ccgagacccc gttcacgcca tatctggctt tcaggatccg gattttatta
     4021 tccgtaaaaa tattgacatc taaagggaag taatcaagac ctgtcttctg ctgtctcgcc
     4081 attacctgcc cttccttttt ctttcaatgc cttcccggcc tctgcttctt tgtacagttc
     4141 catccagtcc acgaaccgca tcgtcactaa ccattcgcac cggtctttcc gatggaatac
     4201 cgccggaaga agccctttgg cggcgtctct gaccgcctgt gataccgcat ccaaaagatt
     4261 cagcttctcc acccgtttgc actctatatg gatgcctagg agccctacca cgtccgcatc
     4321 gccggaggcc ccgcaatact gctgccccct gcggcagtca taaccgtact ccctcaggag
     4381 ccctgccagc tcacgttctc cacgcttgcc tttctctctc tgtgattttc ccatgatact
     4441 ccttcaaaaa aggggcggcg gtcaaaaggt acaaacccgg ccgccccggc caacacctct
     4501 ggtcatttaa taccgtgaca tatcaaatcc caccaaaagg ctgcactgct tatccgatga
     4561 ttgtaatatg gtctcttaca tttggctgca tatgggacac tgcctgcgcc agatatgcct
     4621 tgatatttgc caccgcctca ttcctccaga ggccgttctg tgcctcctga agcataaagg
     4681 taggttctcc cttatctccc acgcggaaca cgaacgcact ggcaggctgc cccacctcct
     4741 ggaaggtacg gtacgggacc agtgtcaccg gattcggcac cttcacattt gcctttgctg
     4801 ctgcgcctac cgtcataaca gccacctggg agatcccatc atctgcatag ctctgatcgt
     4861 tcttgcgctc cacgtttccg gccatcttct ggaccagttc cagatcctca ctgggctgga
     4921 agttggcctg cagctcgatc atgaatgcct cctgatcgta ccagtggccg aaacggaatt
     4981 catttacctc tgcatttacc tcaaacaggc actctcttgt gcgttccgca tccagcgcag
     5041 tgatcagacg cacccgcttg ggatttacga tatggataat catatccgta tccttcggga
     5101 actcctcatg gcaggccccg atgtagccaa gaagggcggt aagactggaa gcctccagtg
     5161 ccgtcgcctt gtctgtgttg tcataacgct ttaagctctt gtttgcatat gtatgtccgc
     5221 agatctcaat gacctcggtc ttctcgtttt ctcttgccag cccctcaacg tgctctaatg
     5281 cagcctttaa tccttccaac atcgttttgt cctccttatt cttctatgtt tcttgcttca
     5341 taacagtcgc agcagtcctg atacatcgta tagtctccgt acccttccga ctcctcacaa
     5401 tcacagatga aatccacatc atcatcatgc agatgatacc tgcaggttcc gcagcatttc
     5461 ttttctgatg ccatttccct ctccttatct tccatatgcc tgtcttaagt caatcgaccc
     5521 ggcctgtacc ggctcttcaa agatctcccc ggtttctggg tcaaactccg gttcctgccg
     5581 gacatccgta taagctgccg caggtgtctc ccttatggca gttacctgcg gaaggccgct
     5641 tcccggctcc gacatgctga tccggccagt ccgcccatcc tgccctacca ggaatacggt
     5701 ctcagatttt ttgaatcctg ccagcttggt cccgactgag aaatcaacct tcagatttcc
     5761 ccggcccatg ggcttaaatt taatatttac cgtcattccc cggacggctt ccggatccgt
     5821 gttaggatcc atgatgttgc gcccgatctg ggtcaaggcc atctggaact tttctgccag
     5881 ctccccgttc gctatcgtat caaacgtgat tgccatatgc tgttctcacc tcctttcctt
     5941 atccaaagaa tgcactggaa gcatcctgag gctgtggtgc cgtctgggac actggctcag
     6001 aagcagaggg ctgtacaggc tctgactgct ctattgccct ctgtgcctgt ggctcaatga
     6061 tatctcccgc tgtataatca ggatcaaccg ctccaacttc ctctgccacg tacattcctg
     6121 caaacgtctt tggaaatgcc tcgcggagag cctgcacaac cgcaacttta cggatcatgg
     6181 ttgcaggctt tttcgaccac tgggaattca ggcttccatc cttcttccgt cccgcatatt
     6241 catcgaaagc aacttcaatg cggaagctgt gacccctgtc cttgcggaat acttctgcgt
     6301 atcccccgac cagtttctca ccatccagtt tcagagttcc ctgacggtaa atgatttcct
     6361 ctgtctcttc ctgctggacg ataataccgg cctccatgcc gtcatagttt tcgttggact
     6421 ctgcccgctt aaaatatgca tctttaccta caaccaaagt cgccggttcg tttccgtact
     6481 taatgcagta cgcttcacgg agccagggat tcaggccggt aaaacggcac aggttgatga
     6541 acattgccac ttcctgatcg gatacccgtt ccttgtcccc gctcaccaga taatttttta
     6601 cggttccggg agtaagcgtt acctccatcc cgtttgccat gtattttgtt acctctacct
     6661 tttgtacggg cttcttagcc agactgttat ttactgccat gattcttttt cctccttaat
     6721 tcttcggtac cggctcaaac cggattccgt tatcatttaa aaatgttttc agcctcatca
     6781 actgtgccat ggtagcgtac acacggaaat caatcacatt gaccggctcc tctaccgtct
     6841 ctatcttctg aggtgtctcc ttaggcggct ccggttccgg tgcccttccg gcctgcataa
     6901 cccggtcggc ctctgccttc cgggtggcct cacgttctgc ctttctccgt tcctgctctt
     6961 ccatgtacag gcgccggttc tccgcctcgg cctccagctg gttccgtctc gccattgctg
     7021 cgccgatatc gtaggtctgt aaaaatacct gcttcatatc cccgacataa ggactgtcca
     7081 cctcattcag gatcgccagc ccctcatcta ccttctggat tagtgcaagg atctcctctt
     7141 tgatggactt tatggtagtg gaagcattgg catactgggg cttcatcacc cggtcaaagg
     7201 gaaggtactg gccgatatcg tggatattag cctcatagaa ctccttaatc tttgccgtct
     7261 tttctttcct ctggcggctc tcatagtcct cgatctggac gtcaatgcta tcaatggcac
     7321 tctggacgat cccggtaagc tccttgatct gctcaccaaa cagctcatcc ggctccagca
     7381 gtttcttacg gatcgcggtc cgttctccct tcacagcctc cacgaactta ttcagtgtgg
     7441 cgcggtcggc ctttgctgcc ttgatggttt catccgtgta gacggttgca gcatattctt
     7501 tggctttggc tgtaacctct tccttcagct cctcgaaatt ccattcgatc ttctttaaat
     7561 atccgccttc ctgcggattg tagattttta attccatacg ttcctcctct gcctttatat
     7621 caccggaaga gcaaaattcg gtctgcggcc ttccaccaca caagcccaga agcgctgttc
     7681 tgcttccacc agataccgga tatcctcttc aacttcttta cgttctataa aataatgctt
     7741 gacggtaatc tgcagttccc catgccattc gcttttaagc tgggccttca aaactgcgaa
     7801 atcatattct gttacagcca ggtaatggag cacctgacaa taataattgt ctggtatctt
     7861 ccccttccat ttttcaagct gcatactctg caaaatattc gtggtcttaa tttccagtat
     7921 cccgcgccgt ccctgctcat ccaccagctc accatccaaa gaagcatgca tccacgggta
     7981 ccggtcgttg atgaacatat tatcttcatc gtagaatacc tgatattccg ggaaatccat
     8041 ggcgaacagt gcccgcagat gtttttctgc ttctgtgccg taccggacat aatccttatc
     8101 tgaaatatcc tccggcatca ccagccccat cttttcttcc cacagctgca cgttgtcttt
     8161 gtatgggttc atacctacac aggcgctggc atccgacccg ccgatatggt tcttccggcc
     8221 cttaagccat tcctcccggc tggcaaatat atgtttagtg actgccatcc gcagcaccct
     8281 ccctcctcat aatctttccg cagttcggac atggtgtgat ctccccaagg agggaccaca
     8341 tccagacacc acaggagcag gctaggaagt aaaacgggga tgtaatcctg acctggctgc
     8401 cggggtatgt agtggggcgg gtcatttgga cagcctttcc aggcatttct ccttcgctgc
     8461 ttcacctacg atacgagcta attctgtcaa tacatgaaca gcacccaaat catcaacagc
     8521 catactgcta actgcatcca ccatactttt ggcaagacat ttagtaaggt catctgagga
     8581 tatgttttct ctctcaccta tgcagatcac cgttgtgtcc actccattct tctccttatt
     8641 gcctgtcaga cagattgcaa aagcaatctc gctttcaact gtttttttga ttccctcata
     8701 taaaattgtt gtgtttacca ttgatttttc tcctgttctc cctcataata gagggtgttg
     8761 attttttaac gcttggaccc gtcacagttg ccgctgtgta ggtccttttt cttcttgtaa
     8821 ttcaggcacg gatacatcct gctccgttct atgcaccgat tcttattgtg gcaagtacca
     8881 caaacatcct tgtccatcgt cctcaccgcc ttaaatctca gaaactgatt cactcaccgg
     8941 gtcataggcc agccgaaatg catcaccatt cccgtctctc agctccgcct cacgggggcc
     9001 gatcatctca aaagcaaagc actccagtgc aatatccccc tgccgctcta actttgccag
     9061 acacttgcgg atcttatatt ccgctacggt tgttgccttc ttcatctttc ctcacctcct
     9121 ctcacagtgc tattattccg gtagccatca aaaacagcac cgctgattct acgcccagca
     9181 ggaacgccat caccgtagac atcctgccga acagccgcac atccttatgg ctctgatcca
     9241 gctgttccct catgatccgg cccagcttca ggatctgctg cttctcttcc tgggtaacta
     9301 caacgccctc cactgggggc tgatacttaa ttacttctga ttgcattttc ttctcctctc
     9361 catatgtatc cagtccactc ccataacttt ttgggactga tatagtaatt ggttcttttc
     9421 cctgattcca tctgtttgag atctgtaacc tgccgtttgt cctgagtcgc tattccaata
     9481 ggcagccatc cagaaatgat tcctgcctga atccaagtag catccttccc gaaaacccgt
     9541 gctgcaacgg ccaccggaac gcctttctct ccaaactcgg gtacagaatt ctcactcata
     9601 agcatacgag ctatatgctc tcctatttcc ttcgcctgag tcagttgtat tacgctctct
     9661 gtcatgctct cacctcccgt cctcagtctc tgcaaaatac tccaccggaa catcaaaata
     9721 cttggcgagg ataagaagtt tatccagttt tggattactt cgaccactct tccaatcaga
     9781 aaaagtagac ttcgtaattc cagtatctat tgatactctg taatctgtca catttctttt
     9841 atctcgtaat gtaacatatt tttcatacat atttttctcc tttccgaact tttctattga
     9901 ttttagttcg gaaatcagtt ataatacatt taccagacat aaaataacaa acaacacttg
     9961 gctgttttga tttccgaact ttgtatttgt attataagac ggatttcaga acttgtcaat
    10021 agttttttgt acggattttc aaacttttaa ggagttcatt atgtacgaaa tttattgcaa
    10081 gttacgaaa
//
LOCUS       DBMDUH010000096        10054 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_3234_length_10054_cov_7.022305, whole genome shotgun sequence.
ACCESSION   DBMDUH010000096 DBMDUH010000000
VERSION     DBMDUH010000096.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 10054)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10054)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10054
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3234_length_10054_cov_7.022305"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="ACZPCG_08895"
     CDS             <1..269
                     /locus_tag="ACZPCG_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285206.1"
                     /translation="DLASRGYCIIMSTHSPEHPASAGTRVLMMKQGSMAAFGSPEEVI
                     TPENLQAVYGIEMDVVTVKDRYGHSRTICMPVKAPVGKERTSLE"
     gene            262..1101
                     /locus_tag="ACZPCG_08900"
     CDS             262..1101
                     /locus_tag="ACZPCG_08900"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR1285207.1"
                     /translation="MNDLAYFISLMEKETEPSPNIWDRRARSWAHERKNNRKTDERLE
                     KDFLFLENHGLLKKDFSVADIGCGPGRFAAAFASRVRFVTALDISGEMIAQGKEYAKS
                     LGLTNLQFHLCDFDTLDLKKEGFKRAFDLVFVSLTPAVRSHIQLLKAMEMSRGRCCCI
                     SHLYRKNRLMEQIMNEVFPEKAMRHENSGRWFYAAFNMLFLMGYKPETSYEPRHSEKQ
                     IVPDEEYLDFLMEKMFLPDDRTAANRNTVMTWLTDHTETDGTITEITDSCYGRLLWEV
                     TNQ"
     gene            1098..1664
                     /locus_tag="ACZPCG_08905"
     CDS             1098..1664
                     /locus_tag="ACZPCG_08905"
                     /inference="COORDINATES: protein motif:HMM:NF015239.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HIR1285208.1"
                     /translation="MTHHVFLTGAKQVGKSTLLKKTLRSFDGQIGGFFTLRTCEYLKT
                     GFSVHLLPAAVPADPDETNLLFICGTSSPLSSNETPRRFDRLGCNALSDFLQKDLILM
                     DELGPHEAEAVRFRQMILKVLDGPVPVLGVLQEAPSPFLQEIAQRPDVTVLRITEENR
                     NEPELLKQLASLLPTLSCQSSVLPARLQ"
     gene            complement(1618..2205)
                     /locus_tag="ACZPCG_08910"
     CDS             complement(1618..2205)
                     /locus_tag="ACZPCG_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007705753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HIR1285209.1"
                     /translation="MDSVSAKKSKKIRHNTAFTTREMVLAGMFAAVLAVISQLSIPTP
                     AGVPVTIQVFGVALVGAVLGWKLGLCSVLVYILIGAAGLPVFANFGGGIRSLIGLTGG
                     YIWAWPVMAVLCGIRPGLKNRISDLAVRIFLSLIGLAAVELIGGLQWAALSGDMTAGA
                     VFAYSMVAFVPKDIVITILGVIVSEPVKQMTDRIG"
     gene            complement(2342..3322)
                     /locus_tag="ACZPCG_08915"
     CDS             complement(2342..3322)
                     /locus_tag="ACZPCG_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HIR1285210.1"
                     /translation="MPGFTTHYILGMKAYNDLPANYLKFVITKYRWLYQLGLQGPDMF
                     FYNLPILRHRDHRNVGSYMHEHRVNDFFRCAFDRLSRIESKQQKEEGMAFLCGFICHY
                     IGDSICHPYVYGRIGYDVQNPGGYYHGLHAKLENDIDALLLKKYKKKKPSQFNQAATI
                     CLNGMETQFISVFLSDCINDAFYRLSSKNHYQVTAGMIHRSILAMRFGCRTLADPNSR
                     KKDRIEFFESLFLKNPIASSKMVTDVVEDPVWSLNLRHEIWCNPWDRSIASRASVPDL
                     LRRCLGKLSTIFYMINEIIEGSKVLRPVAFERLLTELGNYSYHSGLPCND"
     gene            3520..5007
                     /locus_tag="ACZPCG_08920"
     CDS             3520..5007
                     /locus_tag="ACZPCG_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HIR1285211.1"
                     /translation="MKWLKLLHGMVWGPWTLLIFLGTGLFLTVKCRFFQFRGFSFWWD
                     ATAGSIIRGKKNNATNERTPDEGSPETISQFQSVCTALAATVGTGNIAGVATALTAGG
                     PGALFWMWVSAFIGMITAYGETCLGIRFRLREPDGRFLCGPFLYMEHGLNVPFMAVLY
                     SILCVMCALGMGSMVQANSAVTALSFTFNLPLWLPAVCFTLLTGAVIRGGIQSIGRTA
                     ERLVPAAAAIYVLFSLTVILSSLPVLPGVLKDIFDSAFHIRAAAGGAGGFLISRSVRY
                     GIARGVFSNEAGLGTLAVLHGPAEHTTPEKQGMWAMFEVFFDTVILCTLTALVILCMA
                     EAASPRLASLPYEGSALAAWAFKCRLGNLGEAFISASMAVFAFATVIAWFYLGKQALT
                     YLTIRCSLPALFSRFLYPMCFLSAILAGGIIQSGAVWLFSDLWNGLMAFPNLTALLFL
                     SREISLPKTWKKTHSGSRLRYNRKSASPDDDTLFLEKSIIKREES"
     gene            complement(5183..6241)
                     /locus_tag="ACZPCG_08925"
     CDS             complement(5183..6241)
                     /locus_tag="ACZPCG_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007717289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HIR1285212.1"
                     /translation="MRRIDRYWKRCAAGMMAALMLMTAGEGGFTSFAAQSLNSPSKIV
                     ISNTQTQEGNASALQSAPVTTAAFGTVNTPVTDASSYFGAGRMTMLANHDTRAQQLAV
                     IIENGAGGLIVVDGGWQENSAYVLEQIKQRGGHVAAWLLTHPDSDHVGALADILYNHS
                     SEITIDGIYYSFLEDSWYAEKEPAVAAMVSHLKGAFQKVDPSVLHGNIVAGQVIEAGP
                     AKIQVLNQAYAANNDFVNNSSVAYLVSLNGTNVVFLGDMARSGGENLMANVDLGALNC
                     TMVQMAHHGQNGVDYEVYKALRPSVCLWPTPQWLWDNDNGGGTGTGPWKTQETRNWMV
                     RLGVKTYFCTKDGDQVIE"
     gene            complement(6255..6806)
                     /locus_tag="ACZPCG_08930"
     CDS             complement(6255..6806)
                     /locus_tag="ACZPCG_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285213.1"
                     /translation="MFNWKKMSLVLLAAASLLSGCTKSNIVSPEGGTRADDGQPDTEI
                     DKNIKIDWLEVREELRDQYLEPYGAFADYVMDLDARYNEETGVLTVLLPVTHQTTPEA
                     AVAYAQDVLATIGTCVHTQNFYYTEPETNEEGYTTDYGSFFDEHDVLVQVFPYDKEGE
                     VSEYIINDTLKAGDVRALEPVKK"
     gene            7048..8559
                     /locus_tag="ACZPCG_08935"
     CDS             7048..8559
                     /locus_tag="ACZPCG_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006778538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HIR1285214.1"
                     /translation="MKHLTKRLDWLTTLVPFLCILFLCILFIAFPEDSSSVLGSIRFF
                     LGDQFGSFYLLMGLGALFCSLYAAFSRFGSIRLGGKSPRYSNLQWGSMMFTAGLAADI
                     LFYSLCEWMLYAGEPHISDMGSVQDWASVYPLFHWGPIPWSFYIILAVAFGFMLHVRK
                     RNRQKYSEACRPVLGKRVDGALGRIIDLTAVFALLAGTATTFSLATPLLSMALSRVTG
                     IPETPAFTIAILIAICIVYTMAVYFGMKGVARLASSCVGLFFVLLAYFFFGGGEMRYI
                     VETGLTSLGNLSQNFIPLATWTDALRTSSFPQNWTIFYWAYWMVWCVATPFFIGSISQ
                     GRTIRQTVLGGYFFGLSGTFTSFIILGNYGLSLQMHGRLDLMAIYVQSQNLYEAIMAV
                     FETMPFAKAGLLLLALTMIAFYATSFDALTMVASSYSYHSLDEKQEPDRFIKLFWAVT
                     LMLLPIALIFSENSMNNLQTVSIIAAFPISFIILLIIWSFFKDAGSYLNETKK"
     gene            complement(8710..8949)
                     /locus_tag="ACZPCG_08940"
     CDS             complement(8710..8949)
                     /locus_tag="ACZPCG_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HIR1285215.1"
                     /translation="MDIRKGGFNPIPISYLIQTANQFDCDIFVTNESTTANMKHYDEM
                     KRLNMCGFLTFCFKGSDENAAENRIHTILWQNTNG"
     gene            complement(9245..>10054)
                     /locus_tag="ACZPCG_08945"
     CDS             complement(9245..>10054)
                     /locus_tag="ACZPCG_08945"
                     /inference="COORDINATES: protein motif:HMM:NF014385.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR1285216.1"
                     /translation="QSLKKTSSTERQVDRQANSLIMNVQSMMNQYEQLVSQRAMAAKG
                     VELARTARALQDTMQSQGMAVDSDVLSAAASLSSAQSQLASLDAGMEQIYKLLCSFTG
                     YDPASGVTFGAIPSADLAAIDAIDVNADKEKAVNNNYNLISLRSSTGGGMTDFQARTT
                     KTTTQTENRLRNVEYSENAVRSDIQALYDQILEKRAAYDAAKTAYESGKMVWDAAQIQ
                     KQNGSLSQIQYLQQELAWLTTESGYHCAGLELQQAIQNYRWAVAGAAVSVS"
BASE COUNT         2381 a         2622 c         2215 g         2836 t
ORIGIN      
        1 aggatcttgc ttccaggggc tactgtatca ttatgtctac acacagtccc gaacatcccg
       61 cctctgccgg taccagggtg cttatgatga agcaaggctc tatggccgcc ttcggctctc
      121 ccgaagaggt aattactccc gaaaatcttc aggccgttta tggcattgaa atggatgttg
      181 taactgtaaa agacaggtat ggacattccc gaaccatctg tatgcccgta aaagcacctg
      241 tcggaaagga gcggacttct cttgaatgat ctggcctatt ttatctcttt aatggaaaag
      301 gagactgagc cttctcccaa tatctgggac cgccgagccc gttcctgggc tcatgagcga
      361 aaaaacaatc gaaaaacgga tgagcgtctg gaaaaagatt tcctttttct ggagaaccat
      421 gggcttctga aaaaagattt ctcagtggcg gatatcggct gcggaccggg ccgctttgca
      481 gctgccttcg cttccagagt ccgctttgtc acagccttgg acatttccgg tgaaatgatc
      541 gctcagggaa aagaatatgc caaaagcctg ggacttacca atctccagtt ccatctttgt
      601 gattttgata ctctggattt gaaaaaagaa ggcttcaaac gggctttcga tctggtattc
      661 gtttccttaa cccccgcagt ccgcagccac atccagcttt taaaggccat ggaaatgagc
      721 cggggccgtt gctgctgcat ttcccatctg taccgcaaaa accggctcat ggaacagatt
      781 atgaacgaag tctttcccga aaaagccatg aggcatgaaa atagcggccg atggttttat
      841 gccgctttta atatgctgtt tcttatggga tacaaaccag aaaccagtta tgaaccaaga
      901 cacagtgaaa aacagatcgt tccggatgag gaatatctgg atttccttat ggaaaaaatg
      961 tttttgcctg acgatcgtac tgctgcaaac agaaacactg tcatgacctg gctaaccgat
     1021 catactgaaa cagatggcac cataacggaa atcaccgact cctgctatgg acgtcttctc
     1081 tgggaggtaa caaaccaatg acacaccatg tatttttaac cggtgcaaaa caggtgggaa
     1141 aatccaccct tttaaaaaaa acactacgct cgtttgacgg acagatcggc ggctttttta
     1201 ctctgcgtac ctgtgaatat ttaaaaaccg gcttttctgt ccatcttctt cccgccgctg
     1261 tccctgcaga tccggatgaa accaaccttc tctttatctg cggcacgtct tcgccgcttt
     1321 catcaaatga aacgcccaga cggtttgacc gcctgggctg taacgctctg tctgattttc
     1381 ttcaaaagga tctgatcctc atggatgaac tgggccccca tgaagcagaa gcagtccggt
     1441 ttcgtcagat gattcttaaa gtcctggacg gtcctgttcc cgttcttggt gttcttcagg
     1501 aggccccctc tccctttttg caggaaattg cccaaaggcc tgatgtgacc gtcctgcgga
     1561 tcaccgaaga aaaccggaat gaaccggaac ttttaaaaca gctcgcttcc cttctgccta
     1621 ccctatcctg tcagtcatct gttttaccgg ctcgcttaca ataactccca atatggtgat
     1681 cacaatatcc tttggcacaa aagccaccat agaataggca aatacagcgc ctgcggtcat
     1741 gtctcctgaa agggctgccc attggaggcc gccgatcagt tccactgctg ccagaccgat
     1801 aagagagaga aaaatcctca ccgccagatc cgaaatccgg tttttgagtc ccggacggat
     1861 cccgcaaagc accgccatca ccggccaggc ccagatataa cctcccgtaa gccctataag
     1921 cgaccggatg ccgccgccaa aattagcaaa caccggaagc cccgctgccc cgatcaggat
     1981 ataaaccagt accgaacaaa gtcccagctt ccatcccaga accgccccta caagagccac
     2041 tccaaatacc tggatcgtca ccggaacacc tgcgggcgta gggattgaca gctgggagat
     2101 gactgccaga acagctgcaa acattcccgc caataccatt tctcttgtcg taaaagcagt
     2161 gttatgtctt atttttttcg atttttttgc agaaactgaa tccatttcca tccactcctt
     2221 tttccggttc cgttttagat atttcatctg cctgaaacgg aacctgtttt atcttacagg
     2281 aatggtatat cacaggatgt cccaattgtc aaccaattgt attttatggt tgacattgtt
     2341 tttaatcatt gcatggcagg ccgctgtggt aggaataatt tccaagttct gtcaaaagcc
     2401 gttcaaatgc caccggacga agaacctttg acccctctat gatctcattg atcatataga
     2461 agatcgtact cagtttccca agacaccgtc gcagcagatc cggcacagac gctctggagg
     2521 caatgctcct gtcccaggga ttgcaccaga tttcatgacg cagatttaaa ctccaaaccg
     2581 gatcctctac cacgtctgta accattttgc tggaagcaat gggattttta aggaacagcg
     2641 actcaaaaaa ctcaatccgg tcctttttcc ggctgttggg atcggccagc gtgcggcagc
     2701 caaagcgcat ggccagaatc gaccggtgga tcattcctgc tgttacctga taatgatttt
     2761 tgctgctcag acggtaaaac gcatcattga tacaatcgga aaggaatacg gaaataaact
     2821 gtgtttccat cccattgaga cagatcgtgg ctgcctgatt aaactgggag ggctttttct
     2881 ttttatattt tttcagaaga agggcgtcaa tatcattttc cagcttagca tgaaggccat
     2941 ggtagtatcc ccccggattc tgtacatcat agccgatccg tccatagaca taaggatggc
     3001 agatggaatc tccaatataa tgacagataa aaccgcacag aaaagccatt ccctcttcct
     3061 tctgctgctt ggattcaatc ctggaaaggc ggtcaaaggc gcagcggaaa aaatcattta
     3121 cacgatgctc gtgcatatag gagcctacat ttctgtggtc tctgtggcgc aggataggga
     3181 gattgtaaaa aaacatatcc ggcccctgaa gtcccagctg atacagccag cggtactttg
     3241 taatgacaaa cttaagatag ttggcaggaa ggtcattata tgccttcatt cctaatatat
     3301 aatgggttgt aaatcccggc atgagacacc tcttttctgc ctgtggggca gtagtttttg
     3361 ctgttactct tattctatca cactctattt ggaaatgcga ggcggaaatc tgctccattt
     3421 tcttcctgtc atacttttcc cccgctctga atacacagta taaagcctgt ttaaaacacc
     3481 cggagcctgt ctttgggtat atctcttgga ggttttgtta tgaagtggct caagctgctg
     3541 cacgggatgg tctggggacc atggactctt cttatctttc tgggaacagg attatttctc
     3601 acggtaaaat gccgtttttt tcagttccgg ggattttcct tctggtggga tgccaccgcc
     3661 ggaagcatca tccggggtaa aaaaaacaac gcgacaaacg aaaggacgcc agatgaaggt
     3721 tccccggaaa ccatcagcca gttccagtct gtatgcacgg ccctggctgc taccgttgga
     3781 acaggaaata ttgccggtgt tgctactgca cttactgccg gaggccccgg cgccctgttc
     3841 tggatgtggg tttctgcttt tatcggtatg atcacggcct atggggaaac ctgtctgggg
     3901 atccgcttcc ggctccgcga accggacggc cgcttcctgt gcggcccctt tctctatatg
     3961 gagcacgggc ttaatgtccc ttttatggcc gttctctaca gcatactctg cgttatgtgc
     4021 gcccttggca tggggagcat ggttcaggcg aactctgctg ttactgccct gtcctttacc
     4081 tttaaccttc ctctctggct cccggctgta tgcttcacgc tccttacagg agccgtcatc
     4141 cggggaggga tccagagcat cggaaggaca gcagagcgtc tcgtcccggc agccgctgcg
     4201 atctatgtcc tcttttccct caccgtgatc ctttcctctc ttcctgtcct tcccggcgta
     4261 ttaaaggata ttttcgactc tgcttttcat atacgggctg ccgcaggagg agcaggaggc
     4321 tttctcatca gccgcagcgt gcggtacggt attgccagag gggtattttc caacgaggcc
     4381 ggtcttggaa ccctggccgt gctccacggc cccgccgaac acacaacgcc tgaaaaacag
     4441 gggatgtggg ccatgtttga ggtgtttttc gacaccgtga tcctctgcac gctcactgcc
     4501 cttgtcatcc tctgtatggc agaagctgcg tcaccgaggc tggcttctct tccctatgaa
     4561 ggctctgccc tggccgcctg ggcctttaag tgccgtctgg gaaatctggg agaagccttt
     4621 atcagcgctt ccatggcagt atttgccttt gctactgtga tcgcatggtt ttaccttgga
     4681 aagcaggcgc tcacctatct tacgatccgc tgctcccttc ctgccctctt ttcccgtttt
     4741 ctctatccta tgtgcttttt atctgcaatt ttagccggag ggatcataca gtctggagca
     4801 gtctggcttt tttccgacct ctggaacggc ctgatggcct ttcccaatct gactgccctt
     4861 ctgtttttat ccagggaaat ctctctcccc aaaacctgga aaaagacgca ctcaggatcc
     4921 aggcttcggt ataacagaaa aagcgcatca ccggatgatg atacgctctt tctggaaaaa
     4981 agtattatta aaagggagga gagctgataa tcacttatca gctcgagtat tatgaaaaaa
     5041 atcttgtaag catattgggg atagatgaat gtttctctct catctatgat taaagtatac
     5101 ccatgaaaga tgaagaaaaa atgttcattg tgtaaaacct ttttgaatga attatgaaca
     5161 gaatgtgaat atccctattc gcctactcaa ttacctgatc tccgtccttt gtacagaaat
     5221 aggtctttac gcccaaacgg accatccagt tacgggtttc ctgggttttc cacggtccgg
     5281 ttcctgttcc gccgccgttg tcattatccc aaagccactg aggtgtgggc cacaggcata
     5341 cggatggacg gagcgccttg taaacttcgt aatcaacccc gttctgaccg tggtgagcca
     5401 tctgtaccat ggtacagttt aatgccccca gatctacatt ggccatcagg ttttcaccgc
     5461 cggatctggc catatctcct aaaaatacga cattcgtacc atttaaggat acaaggtagg
     5521 ccacgctgct gttattgaca aagtcattat ttgccgcata tgcctggttt aatacctgga
     5581 ttttggcagg gcctgcctcg atcacctgtc cggccacaat atttccgtga agaacagagg
     5641 gatctacctt ctgaaaggct ccctttaagt gggataccat ggcagccacc gccggctcct
     5701 tctcggcata ccagctgtcc tctaaaaagg aataataaat gccgtcaatg gtaatctcac
     5761 tgctatggtt ataaagaata tctgccagcg ctcccacatg atcggaatcc ggatgggtaa
     5821 gcagccatgc tgctacatga ccgcctctct gcttgatctg ctccagtaca taggcgctgt
     5881 tttcctgcca tcctccgtct accacgatca gtccgccggc tccattttcg ataattacgg
     5941 ccagctgctg ggctcttgtg tcatggtttg ccagcatggt catgcgtccg gctccaaaat
     6001 aggaggatgc gtctgtcacc ggcgtattta ccgttccaaa agctgccgta gttaccgggg
     6061 cgctctgtaa tgcagaagcg tttccttcct gggtctgtgt attgctgatc acaatcttgg
     6121 aaggggaatt gaggctctgg gccgcaaagc tggtaaaacc tccctctccc gccgtcataa
     6181 gcataagagc agccatcatg ccggccgcgc agcgtttcca atatcggtcg atccttctca
     6241 tggcgtctgc ctccttattt cttcacaggc tcaagagctc ttacatctcc cgcctttagg
     6301 gtgtcattga taatatactc ggatacctct ccctctttat cataagggaa tacctgaacc
     6361 agcacatcat gctcatcaaa aaagctgccg tagtcggtag tgtagccttc ttcgtttgtc
     6421 tcaggctccg tgtaatagaa attctgggta tgtacacaag tgccgatggt agccaggaca
     6481 tcctgcgcat aagcgactgc ggcctccggc gttgtctggt gcgttacagg cagcagtacc
     6541 gtcagaacgc ctgtttcttc attatagcgt gcatccagat ccatcacata atcagcaaac
     6601 gcaccatatg gctccagata ctgatcccgc agctcctccc gcacttccag ccagtcgatt
     6661 ttaatatttt tatcgatctc tgtatcaggc tgtccgtcat cggctcttgt acctccctca
     6721 ggagacacga tattactctt ggtacatccg cttaaaagtg aagcagccgc taaaaggacc
     6781 agagacattt tcttccagtt aaacatttca aaaacctcct ttgttcatag cgttactatt
     6841 ataacggaca aactgaaaat ttaacagacc ttttttctta caaaattgtt acacattcga
     6901 aaaaggattc tgtatacaca tacagttttt ttgaaaaact tcttgatttt tccaaaatgc
     6961 ggtggtatta tgttcaatat gctttatatt acttaatttt tgaacaaaaa acgatacaag
     7021 cctgtatcat caaaggaggt tatcattttg aagcacctta caaaaaggct ggactggctt
     7081 accacccttg ttccttttct gtgcatcctt tttttgtgta tcctttttat tgcctttcct
     7141 gaagactcct cttctgtgct gggatccatc cgcttctttc tcggagacca gtttggaagc
     7201 ttttatcttt taatggggct gggagcgctt ttctgttctc tctatgctgc attttcccgt
     7261 tttggctcca tccgcctagg cggcaaatcc ccccggtatt ccaatctcca gtggggctcc
     7321 atgatgttta ctgccggcct ggcagccgat atcctgtttt attccctctg cgagtggatg
     7381 ctctatgcgg gagaacctca tatctccgat atgggttctg tgcaggactg ggcctctgtt
     7441 tacccgctgt ttcactgggg accgatcccg tggagttttt atatcatttt agctgttgcc
     7501 tttggcttta tgctccatgt ccggaaacgg aaccgtcaga aatactccga agcctgccgt
     7561 cctgttttgg gaaagcgggt agatggagct ttgggacgca tcattgacct cactgccgta
     7621 tttgccctgt tagccggaac agctaccact ttttctctgg caacgcccct tttatccatg
     7681 gctttaagcc gtgtaacggg gattccggaa acgccggcct ttacgattgc gatcctgatc
     7741 gccatctgca ttgtatatac aatggccgtg tactttggca tgaaaggggt ggcccgcctg
     7801 gcttcttcct gtgtgggact ttttttcgtc ctcttagcct attttttctt tggcggcggg
     7861 gaaatgcgct atatcgtaga aacaggtctt acctctttgg gaaacctgag ccagaatttc
     7921 atccctctgg caacctggac cgacgctctg cgcacctcct cctttccaca gaactggact
     7981 attttttact gggcctactg gatggtgtgg tgcgtggcga ctccgttttt tatcggcagc
     8041 atcagccagg gacgtaccat acggcagacc gttttgggtg gatatttctt cggactgtcc
     8101 ggcaccttta cttcttttat cattcttgga aattacgggc tgtcccttca gatgcatgga
     8161 aggctggatc tgatggcaat ttatgttcaa tcccagaacc tctatgaagc aattatggcg
     8221 gtttttgaaa ccatgccttt cgccaaagca ggccttcttc tcctggccct taccatgatc
     8281 gctttttacg ctaccagctt tgatgcctta actatggtcg cttcctccta ctcctaccat
     8341 tctctggatg agaaacagga accggaccgc ttcatcaagc ttttctgggc tgtaacgctg
     8401 atgcttcttc ccattgctct gatcttttca gaaaactcca tgaacaacct gcagaccgta
     8461 tccatcattg cagcctttcc catcagcttt atcattcttc ttattatatg gagtttcttt
     8521 aaagatgcgg gatcctatct gaatgaaaca aaaaaataag caacaaaaaa gagcaggaaa
     8581 aactgacata tcacctgctc tttcttttat tttatgtgcc gctaggcgca ccaccacaca
     8641 aaaacaggag gcctggtcac acagcctcct gtcattctgt cttgtgcatg aaatgctaca
     8701 cttccttact tatccgttag tattttgcca taaaatggta tgtatcctgt tttctgccgc
     8761 attctcgtcg cttcccttaa agcagaatgt aagaaaacca cacatattta atctcttcat
     8821 ttcatcgtaa tgtttcatat ttgctgtcgt actttcattg gttacaaaga tgtcgcagtc
     8881 aaactgattc gctgtctgaa tcagataaga aatcggaatc ggattaaatc ctcctttcct
     8941 tatgtccata cgccgtgtta ccatagactc acctcaattc acaagaaaca aaacgtgcag
     9001 gttaccttcc tcctttaact taaaatatat tccccttata aaacataagg gcatcttttc
     9061 acaacaaaat caatgacgtc atttatattt ttatgcagtc ttaataactt tcattctttt
     9121 ttctctgttt acacaaaaga atcccacaag cgggattctc cgcctgcggc gggccgcatc
     9181 agcggcataa tcccactccg ccgcagtgcg gtccccgaag ggatttttta attattaact
     9241 gtatttatga aacactcaca gctgcgccgg ctacagccca tctgtaattc tggattgcct
     9301 gctgaagttc cagacctgca cagtggtatc ctgattctgt ggtcagccat gcaagttcct
     9361 gctgaaggta ttggatctgg cttaaacttc cgttctgctt ctggatctga gccgcatccc
     9421 agaccatttt tccgctttca taagcggttt ttgcagcatc ataggcagcg cgtttttcca
     9481 ggatctgatc gtacagtgcc tgaatatcgg aacggactgc attttctgaa tactctacat
     9541 ttcttaaacg gttttcagtc tgtgtagtcg tctttgtagt acgggcctga aaatcggtca
     9601 taccgcctcc ggtagagctt cttaagctga tcagattata gttgttattt actgcctttt
     9661 ccttatctgc atttacgtca atggcgtcaa tggccgccag atctgcagag gggattgctc
     9721 caaaggtaac gccggaggca gggtcatatc ctgtaaagct gcagagaagc ttatagatct
     9781 gctccattcc cgcatccaga ctggccagct gggactgcgc agaggaaaga gaagccgctg
     9841 cggataaaac atcgctgtcc accgccatcc cctgagactg catggtatcc tgaagcgctc
     9901 tggctgtccg ggccagttcc actccctttg cagccatggc acgctgggag accagctgct
     9961 catactgatt catcatggac tggacattca tgatcaggga attggcctgg cggtctacct
    10021 gacgttccgt agatgaggtc ttttttaagg actg
//
LOCUS       DBMDUH010000097         9990 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_3249_length_9990_cov_5.387501, whole genome shotgun sequence.
ACCESSION   DBMDUH010000097 DBMDUH010000000
VERSION     DBMDUH010000097.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 9990)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9990)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9990
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3249_length_9990_cov_5.387501"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /locus_tag="ACZPCG_08950"
     CDS             <1..828
                     /locus_tag="ACZPCG_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005540336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HIR1285217.1"
                     /translation="AAIQGGKLAGDAGAGDILLLDVTPLSLSIETMGGVATRLIERNT
                     TIPTKKSQIFSTAADNQTAVDIHVVQGERQFARDNKTLGQFRLDGIPPARRGIPQIEV
                     TFDIDANGIVNVSAKDLGTGKEQHITITSGSNMSDADIDKAVKEAAEYEAQDKKRKEA
                     IDARNDADSMVFQTEKALEEVGDKIDGNDKAEVEADLNALKEAINRAPVDQMTDAQVE
                     DIKSGKEKLMNSAQKLFAKVYEQAQQAGAAGAGPGMGGAAGGSRPDDDVVDADFKEV"
     gene            997..2157
                     /gene="dnaJ"
                     /locus_tag="ACZPCG_08955"
     CDS             997..2157
                     /gene="dnaJ"
                     /locus_tag="ACZPCG_08955"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006781889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HIR1285218.1"
                     /translation="MAESKRDYYEVLGVSKDADDDALKKAYRKLAKKYHPDANPGDKE
                     AEAKFKEASEAYSVLSDPQKRQQYDQFGHAAFEQGGGGAGGFGGFGGFDFGGQDMGDI
                     FGDIFGDIFGGGRSSRGRSGPSRGANVRTAVRITFEEAVFGCEKEIEINFKEICSSCN
                     GTGAKAGTSPQTCTKCNGKGKIMYTQQSFFGQVQNVQTCPECGGTGKVIKEKCPDCYG
                     TGYKTVRKKFKVSIPAGIDNGQCVRMAGGGEPGINGGERGDLLVEAVVSQHPIFKRQD
                     TSIYSTVPISFARAALGGTIRIKTVDGEVEYEVRPGTQTDTKVRLKGKGVPSLRNRNV
                     RGDHYVTLVVQVPERMTQAQKEALKRFDEAMNGGASEEGKPKKKGFKEFFEK"
     gene            2666..3574
                     /locus_tag="ACZPCG_08960"
     CDS             2666..3574
                     /locus_tag="ACZPCG_08960"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009226 - nucleotide-sugar biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR1285219.1"
                     /translation="MKKRILITGSGGFLGSRLAEYFEGKDGYETVAVTHEELDITDMV
                     AVSAFFKAIRPQAVLHCAGLSNTGQCEADPLASGRINVQGTENIARACRESGCRLIFM
                     SSDQVYAGTDTLEPNRETDRVKPVNVYGRDKKRAEEAALALAEGAVCLRLTWMYDYPV
                     PKRTPGTGLLGQMFAAAGEGKVIVSPTHDYRGITYVWDVIRAMEQVMELPGGIYNFGN
                     RNQRSTWETAGIFAQAVSVKTGVLPKVEADRDRFRSLPRNLTIDTGKIEALGIFLGDT
                     AEGLSRCMKDNGVCWKWKKEQLSARG"
     gene            3622..4245
                     /locus_tag="ACZPCG_08965"
     CDS             3622..4245
                     /locus_tag="ACZPCG_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285220.1"
                     /translation="MGQKDIKKDQAPKRPQKVSGQALKICGMTLICLGVMFAVAWVNR
                     NRQENRLAEEYTISAENPRHIRFEEMPEGEAEMTAGLFFRYHAMAQYEECSSLLSKDQ
                     AEFMNFPQQEEDFRSGSYIKDYLIHSFETLPEEEYAGSKEYYDGRAALLGYEEYRVVR
                     VVFHQTWTDQAIKNRQLSGDGEYIREMAVGKGRDGWKIFEAGNPQEL"
     gene            complement(4242..5147)
                     /locus_tag="ACZPCG_08970"
     CDS             complement(4242..5147)
                     /locus_tag="ACZPCG_08970"
                     /EC_number="3.2.2.-"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003505966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase family protein"
                     /protein_id="HIR1285221.1"
                     /translation="MKYTCTIPCFDLEQTALSGQCFRLMPGKEPGLWSVISQGKLLSI
                     RQNDSQFTFECEEGSLEYWLSYFDVSTDYQAMIDSIDPDDTYLLSAAKAGAGIRILRQ
                     DPWEMIITFVISQQKTIPAIRSLVEALCRNYGTHIERTLLASSKLSEAGPSPFAFPTP
                     EQLSRASLDDLLALKLGYRAKYIHRLCQDALEGSLNLDLLSSQNYKEAIDYLTSFYGI
                     GKKVANCVCLFGLHHIDAFPVDTWIQKILMEHYFDEKKYRRIPKSQLFDRIIEDSFGC
                     YPGYAGVMQQYIFNYERNVIGKNQT"
     misc_binding    5389..5647
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5741..6760
                     /gene="pheS"
                     /locus_tag="ACZPCG_08975"
     CDS             5741..6760
                     /gene="pheS"
                     /locus_tag="ACZPCG_08975"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HIR1285222.1"
                     /translation="MKEQLEKIKEEALQQISSAESLDTLNDIRVAYLGKKGELTSLLK
                     SMKDVAPEDRPKVGQLVNEARAVIEGRLEEAKAALAKKAREEQLKREVIDVTLPAKKN
                     SVGHSHPNTIALEAVERIFVGMGYEVVEGPEVEYDEYNFTKLNIPPNHPAKDEQDTFY
                     ISKDIVLRTQTSPVQARTMETGKLPIRMISPGRVFRSDEVDATHSPSFHQVEGLVIDK
                     NITFSDLKGTLAEFAREMFGEDTKVKFRPHHFPFTEPSAEMDVSCFKCGGKGCRFCKG
                     SGWIEILGCGMVHPHVFEMCGIDPQEYTGFAFGVGLERIALLKYEIDDMRLLYENDIR
                     FLKQF"
     gene            6793..9225
                     /gene="pheT"
                     /locus_tag="ACZPCG_08980"
     CDS             6793..9225
                     /gene="pheT"
                     /locus_tag="ACZPCG_08980"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002575007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HIR1285223.1"
                     /translation="MNTALSWIKAYVPDLDVAAQEYTDAMTLTGTKVEGFTCLDKNLE
                     KIVVGEVLSVERHPDADKLVVCQVNVGEAEPVQIVTGAPNITKDSIGAKVPVVLDGGR
                     VAGGHDGGPLPEEGIKIKKGKLRGVESFGMMCSIEELGADRNMYPDAPESGIYLLPED
                     SVPGDDAVRILGLRDVVFEYEITSNRVDCYSVVGIAREAAATFRKPFFPPVVTATGNN
                     EDIHDYLKVSVENTDLCKRYCARMVKNIKLAPSPEWMQRRLAASGIRPINNIVDITNY
                     VMEEYGQPMHAFDYDQLAGHEIVVKCASEGDVFQTLDGQDRILDSTILMINDGEKQVG
                     IAGIMGGENSKITDNVSSMVFECACFDGTNIRLSAKKLGLRTDASGKYEKGLDPNTAE
                     EAVNRACQLIEELGAGEVVGGMIDVYPVKRGETRIKFEPEKINKLLGTDISKEDMLAY
                     FEKIDLGYDEASGEVVAPSWRQDLLRMADLAEEVARFYGYDKIPTTLPSGEATTGKLS
                     YKLMIEDMARETAEFCGFSQGMTYSFESPKVFDKLLIPEDSELRKAITISNPLGEDFS
                     IMRTLPLNGMLVSLSTNYNRRNKEVRLYELANVYRPKALPLTELPDERMQFTLGMYGS
                     GDFFDMKGVVEEFFDKVGMDKKVHYDPKGDHPYLHPGRKADIVYEGRTVGFLGEIHPE
                     VAENYKIGDRAYVAVIDMPAMTEFTTFDRKYTGIAKFPAVTRDISMVVPKAVLVGQIE
                     DVISQRGGKILESCKLFDIYEGAQVLEGHKSVAYSITFRAKDHTLEEKEVTAVMNKIL
                     NGLKNLGIELRA"
     gene            9300..9728
                     /locus_tag="ACZPCG_08985"
     CDS             9300..9728
                     /locus_tag="ACZPCG_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285224.1"
                     /translation="MDGKSRSYMRAVCAVLAEGLLAAGCGKEETAAAETAEIAVPEAA
                     KRTEGSETTASAEFFSEFKNTVFIFSSGAGAWRTELQIREDGSLSGRFEDANMGEIGE
                     ENPQGTIYESVFTGRLGSPVRLDENSYQVDILEMEYENPL"
     gene            9825..9962
                     /locus_tag="ACZPCG_08990"
     CDS             9825..9962
                     /locus_tag="ACZPCG_08990"
                     /inference="COORDINATES: protein motif:HMM:NF018684.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HIR1285225.1"
                     /translation="MKDKETDADEAGKEFEGGSMQLMAYSLKAMEITRERVYELMELL
                     D"
BASE COUNT         2741 a         1968 c         2958 g         2323 t
ORIGIN      
        1 gctgcaatcc agggcggaaa actggcaggc gatgcaggcg ccggagatat ccttcttctg
       61 gatgttaccc cactgtccct gtccattgag accatgggcg gagtagctac cagactgatt
      121 gagagaaata caaccattcc tacaaagaag agccagattt tctctactgc tgctgacaat
      181 cagactgctg ttgatatcca tgtggtacag ggtgagcgtc agtttgcaag agataacaaa
      241 actcttggac agttccgtct ggatggaatt ccgccagcaa gaagaggtat ccctcagatt
      301 gaggtaactt ttgatattga tgccaatggt attgtaaatg tatctgcaaa ggatctggga
      361 accggaaaag agcagcacat caccattacc tccggctcca atatgtctga cgctgatatc
      421 gacaaggctg taaaggaagc tgctgagtat gaggctcagg ataagaagcg caaggaagcc
      481 attgacgcca gaaatgacgc cgactccatg gtattccaga ctgagaaggc cttagaagag
      541 gttggagaca agattgatgg caatgataag gcagaggtag aggcagactt aaatgcactg
      601 aaggaagcaa tcaaccgtgc tccggtagat cagatgaccg atgctcaggt agaggatatc
      661 aagtccggca aggagaagct gatgaacagc gctcagaagc tctttgcaaa ggtatatgag
      721 caggcacagc aggcaggagc agcaggtgca ggccctggca tgggcggcgc agcaggcggc
      781 tccagaccgg atgatgatgt agtagacgca gactttaagg aagtgtaagg aagaaaggat
      841 tcccgcctgc agcagggcgg gaatccatta tataaaaccg taaggccaga ggtccggtcc
      901 attgggcgag atgccccctg gccggaccta aaggtctgtc cggggatatt ttatttaaga
      961 gaaaatgttc tgatagatat gtaagaggta aacaacatgg cagagagtaa gagagattac
     1021 tatgaggttc tgggcgtttc caaagacgcg gatgacgatg ccctgaaaaa ggcatacaga
     1081 aagctcgcca aaaaatatca tccggatgcc aatcccggag acaaggaggc agaggccaaa
     1141 tttaaagagg catctgaggc ttacagcgtg ctcagcgatc ctcagaagcg ccagcagtat
     1201 gaccagttcg gccatgccgc ctttgagcag ggaggcggag gcgcaggagg gtttggcggc
     1261 ttcggcggtt ttgactttgg cggtcaggat atgggagata tttttggaga tatttttgga
     1321 gatatctttg gcggcggccg ttcctccaga ggaagaagcg gtccttcaag gggcgcaaat
     1381 gtaagaaccg cagtgaggat cacctttgaa gaggctgtgt tcggctgtga gaaggaaatt
     1441 gagattaatt ttaaggagat ctgttcttcc tgtaacggaa caggcgcaaa ggcgggtacg
     1501 tcgccccaga cctgtacaaa atgtaatgga aagggtaaga tcatgtatac ccagcagtcc
     1561 ttctttggac aggtacagaa tgtacagacc tgtcctgagt gtggcggcac aggcaaggtg
     1621 atcaaggaaa agtgtccgga ttgctatggt acggggtata agacagtccg caagaagttt
     1681 aaggtgtcca ttcctgccgg tattgacaat ggtcagtgcg ttcgtatggc cggaggcgga
     1741 gagcccggta tcaacggcgg tgagagaggc gacttgctag tggaggcagt tgtttcccag
     1801 catccgatct ttaagcgtca ggatacaagc atttattcta ccgtgccgat ttcctttgca
     1861 agagcggctt taggtggtac gatccggatt aagacagtgg acggagaagt ggagtatgag
     1921 gttcgtcctg gtacgcagac tgatacgaag gtgcggctga aggggaaggg tgtgccttcc
     1981 ttaagaaacc ggaatgtgag aggggatcac tatgtgactt tagtggttca ggttcctgag
     2041 agaatgaccc aggctcagaa ggaggccctg aagcggtttg atgaagctat gaatgggggg
     2101 gcttcggagg aagggaagcc taagaagaag gggtttaaag aattttttga aaaatgaaga
     2161 tgagatgcgc tgagtgcagg cgcgtttcgt gctttgactg atgtgagcgg gagaccgccc
     2221 tggaattgct cttgttggat ggttttgtgt gaggatgacg agagacgttt tgggggcggt
     2281 tttctttttt tgtggggagg atgttgttgg agggggggga tgcggaaggg gaaaagcttt
     2341 atctttggga gcgaggggat gcgggagctg gttttttagg gatcgggcgg ggatcttctt
     2401 gacccgcttg cgggtcaaga agcatcggat gttcatccga tgtgaaatcg tgcaggaaca
     2461 gctccttaca agcaagcttg acggggctgt ttctgcacga tttcgcgccg tcctgaattt
     2521 ttgagaaaaa ttaagaaaat cgcaaagatt ctggggggga gtatgggtat aatggtagat
     2581 atatttttac ctgggactgc cggatgacgt gggatttccg gggactgggt aaggcttatg
     2641 aggaagaaag gctgttggtt atagaatgaa aaaacggatt ttgatcacgg gttcaggagg
     2701 atttctgggg tcgagacttg cggaatattt tgaggggaag gatgggtatg agacggtagc
     2761 ggttacccat gaggagctgg atattacaga tatggtggcg gtgtctgcat tttttaaggc
     2821 gattcgtcct caggcagtgc tgcactgtgc aggcttgtct aatacgggac agtgtgaggc
     2881 ggatcccctg gcttcgggac ggatcaatgt tcagggaaca gaaaatatag caagagcctg
     2941 cagggagagc ggctgcaggc tgatctttat gagttcggat caggtttatg cgggaacaga
     3001 tactctggag ccgaatcgtg agacagaccg ggtgaagccg gtgaatgtgt atggaaggga
     3061 taaaaaacgg gcagaggagg cagcgcttgc tttggcagaa ggggcggtct gtctgcgttt
     3121 gacctggatg tatgattatc cggttccgaa aaggacgccg ggaaccgggc tgttaggaca
     3181 gatgtttgca gccgccgggg aaggaaaggt gattgtaagc ccgacccatg attatagagg
     3241 catcacctat gtgtgggatg tgatccgggc tatggagcag gtgatggaac tgccgggagg
     3301 aatctataat ttcgggaaca ggaatcagag aagcacctgg gaaaccgccg ggatttttgc
     3361 tcaggcagta tctgtaaaaa caggagtgct gccaaaggtg gaagcggaca gggatcgttt
     3421 tcgttcgctc cccagaaatc tgacgatcga tacaggtaaa atagaagcct tggggatttt
     3481 ccttggggat acggcagagg gattatcccg atgcatgaag gataacggag tctgctggaa
     3541 gtggaagaaa gagcagctgt ctgcccgggg ataaaggccg gacagaagaa ttgccataac
     3601 aaaaaggagg ttctgtcgaa cgtgggacag aaggatataa aaaaagatca ggcaccaaaa
     3661 cggccgcaga aggtatcggg acaagctctt aaaatctgcg gaatgacatt gatctgtctg
     3721 ggagttatgt ttgccgtggc ctgggtgaac cggaaccgcc aggagaaccg tctggcggaa
     3781 gaatatacga tttcagcgga aaatccccgc catatccgct ttgaagagat gcctgaggga
     3841 gaggccgaaa tgacagcagg tctgtttttc cgctatcacg ccatggctca gtatgaggaa
     3901 tgcagcagcc ttctttcaaa ggatcaggca gagtttatga attttcccca acaggaggag
     3961 gattttagaa gcggctccta cataaaagat tatttgatcc acagctttga aacgcttccg
     4021 gaggaggaat atgcgggaag caaagagtat tatgacggca gagcagccct tttgggatat
     4081 gaggaatacc gtgtagtccg tgtggtcttt catcagacct ggacagacca ggccattaaa
     4141 aacagacaat taagcggaga tggagaatat atccgggaaa tggctgtggg aaagggaagg
     4201 gacggatgga aaatatttga ggccggaaac cctcaggagc tttaagtctg gttttttcct
     4261 attacatttc tctcatagtt gaaaatatac tgttgcatta cccctgcata gcccggatag
     4321 catccgaagg aatcttcgat gattcggtca aacagctggg atttggggat gcggcggtat
     4381 tttttttcgt caaaataatg ttccatgagg attttctgga tccaggtatc gacaggaaat
     4441 gcatcaatat gatggagacc gaagaggcag acgcagttcg ctaccttttt accgattcca
     4501 taaaagctgg tgagataatc tatggcctct ttatagttct gggaggacag aagatccagg
     4561 ttcaggcttc cttccagtgc gtcctgacag agccggtgga tatattttgc ccggtatccc
     4621 agcttcagtg ccagcaggtc atccaatgag gcgcggctta actgttccgg cgtaggaaag
     4681 gcaaaggggg agggaccggc ttcagaaagc tttgaggatg cgagcaatgt gcgttcaatg
     4741 tgcgttccat aattacggca cagagcttca actaaggagc ggatggccgg gatggtcttt
     4801 tgctgggaga taacaaaggt gatgatcatt tcccatggat cctgacggag aatacgaatg
     4861 cctgcacctg ctttggcggc agataaaaga taagtatcat ccggatctat ggaatcaatc
     4921 atggcctggt agtctgtgga cacatcaaaa taggacagcc agtattccaa agaaccttct
     4981 tcgcattcaa aggtgaattg ggaatcgttt tgcctgatgg ataaaagctt cccctggctg
     5041 atcacggacc acagtccggg ctcttttcct ggcatcagcc ggaaacactg accggagaga
     5101 gcggtctgct ctaagtcaaa gcaggggatg gtacaggtat atttcatgga aatcatcctt
     5161 tcttttgtga aaatgatggt tctatgatag ctggaaagga gagaaaggtc aaatgaaaaa
     5221 tgagtttctg tactctgcac cggcctctgg agtttggtgt gctggactcc ggtttccggt
     5281 cataggagaa cctaactcca gcgactggtg caaaatgtga aaacgcagga aaaaatgggg
     5341 gttgactttt ctagatgaaa cggctaaaat ttacagtaga ttttatatct aaaagcgatg
     5401 agagtgtaca gtaggtactt atgttctcac agagagggaa ggtcgtcggc tggaagcgtt
     5461 cccgagttca cataagcgct gaatttccca ccggagctgc atgggtgaaa gggcgtctgc
     5521 tcgcagcctg tgacgtaggc gtgcgttaag cgtgtttaag tgtctgcaaa ggatagaaag
     5581 ccttggctta gtatctgtat tgcaggaatc agggtggtac cgcggaatga tttgatccgt
     5641 cccttcagga atctgaaggg gcggtttttt gttatggcag caaacatttc cgcgaaaaga
     5701 accgtacact tatttaaaag agaacagaaa aggagaaccc atgaaagagc agttagagaa
     5761 gatcaaagaa gaggcgctgc agcagatttc ttccgctgag agccttgata cgctgaatga
     5821 catccgtgtc gcttatcttg gaaagaaggg agagctgacc agccttttaa agagcatgaa
     5881 ggatgtggct ccggaagacc gtccaaaggt gggacagctg gtaaatgaag ccagagctgt
     5941 gatcgagggc cgcttagagg aagcaaaggc tgccctggca aagaaggcca gagaagagca
     6001 gttaaagaga gaggttattg acgttaccct tcctgcaaag aagaacagcg taggccacag
     6061 ccatccaaat accattgcat tagaggcagt ggagcggatt ttcgtgggca tgggctacga
     6121 ggtagtagaa ggccctgagg tggagtatga tgagtacaac tttaccaaat taaatatccc
     6181 gccaaaccat ccggctaagg atgagcagga taccttctat atcagcaagg acattgtgct
     6241 ccgtacccag acctccccgg tacaggccag aaccatggaa acaggtaagc ttcctatccg
     6301 tatgatctct ccgggccgcg tattccgttc cgatgaggtg gatgcaaccc attctccatc
     6361 cttccatcag gtcgagggac tggttattga taaaaatatt acattctccg atttaaaggg
     6421 aactctggca gagtttgcac gagagatgtt cggcgaggat acaaaggtta aattccgtcc
     6481 ccaccatttc ccatttaccg aacccagcgc cgagatggac gtaagctgct ttaagtgcgg
     6541 cggaaaaggc tgccgtttct gcaagggctc cggctggatt gagattttag gctgcggtat
     6601 ggtacatccc catgtatttg agatgtgcgg cattgatccc caggaatata caggctttgc
     6661 tttcggcgta ggcctggagc gaattgccct gttaaaatat gagatcgacg atatgcgcct
     6721 tctctatgaa aatgatatcc gcttcttaaa gcagttttaa ttgttccatc ataaataaaa
     6781 aggagaaccg acatgaatac agcattatca tggattaaag catatgttcc tgacctggat
     6841 gtggctgccc aggagtatac agatgcgatg acattgacag gcaccaaggt tgaaggattt
     6901 acctgtttag ataaaaatct ggaaaagatc gtagtaggag aagttctttc cgtagaacgt
     6961 catccggacg ctgacaagct ggtcgtgtgc caggtcaacg taggggaagc agagcccgta
     7021 cagatcgtaa ccggagcgcc aaatattaca aaggattcca tcggtgcaaa ggtaccggtg
     7081 gtactggacg gcggacgagt agcaggaggc catgacggcg gcccccttcc ggaggaaggc
     7141 attaaaatta aaaagggcaa attaagaggc gtggagtcct ttggaatgat gtgctccatc
     7201 gaagagctgg gcgctgaccg gaatatgtat ccggatgccc ctgaaagcgg catctacctg
     7261 ctccccgagg acagtgttcc cggcgacgat gcagtgcgga tcctgggtct gagagacgtg
     7321 gtgtttgaat acgagattac ctccaaccgt gtagactgct acagcgtagt gggaatcgca
     7381 agagaggcag ccgctacctt ccgcaagccc ttcttcccgc cggtagttac agctacgggc
     7441 aacaatgagg acattcacga ttacttaaag gtgagtgtgg agaatacaga cctgtgtaag
     7501 cgctactgtg ccagaatggt aaagaatatc aagctggctc cttcaccgga gtggatgcag
     7561 cgccgtctgg ctgccagcgg aatccgtccc atcaacaata tcgtggatat caccaactat
     7621 gtgatggaag agtacggaca gcccatgcac gcctttgact atgaccagct ggcaggtcat
     7681 gagatcgtgg taaaatgtgc ttctgagggg gatgtattcc agactctgga cggacaggac
     7741 agaatactgg attctaccat tttgatgatc aatgacggtg aaaagcaggt gggaatcgcc
     7801 ggtatcatgg gaggcgaaaa ctccaagatc acagacaatg tgtcctctat ggtatttgag
     7861 tgcgcctgct ttgacggcac caatatccgt ctttccgcaa agaagctggg cctgcgcacc
     7921 gatgcgtccg gcaagtatga aaagggactg gatcccaaca ctgcagaaga ggctgtaaat
     7981 cgtgcctgcc agctgatcga agagctggga gctggagagg ttgtaggcgg aatgattgat
     8041 gtttatcctg taaagagagg ggagacaagg atcaaatttg agcctgaaaa gatcaataag
     8101 cttctcggca ccgatatttc aaaggaagat atgctggcat attttgagaa aatcgacctg
     8161 ggctatgatg aggcttccgg tgaggtagtc gctccaagct ggcgtcagga tcttcttcgt
     8221 atggcagatc tggcagagga agtggcaaga ttctatggct atgacaagat tccgaccaca
     8281 cttccatccg gtgaagcaac cacaggaaag ctttcctata agttgatgat cgaggatatg
     8341 gcaagagaga cagcagaatt ctgcggcttc tcccagggta tgacatattc ctttgaaagt
     8401 ccaaaggtat ttgacaagct tttaattcct gaggacagtg agttaagaaa ggccattacc
     8461 atttccaatc ctctgggcga ggatttcagc atcatgcgta ccctgccttt aaacggtatg
     8521 ctggtatcat tatccaccaa ctacaaccgc cgcaacaaag aggtgcgttt gtatgagctg
     8581 gcaaatgtat accgtcccaa ggcacttcct ttaaccgagc ttcccgatga gcggatgcag
     8641 tttactctgg gtatgtatgg aagcggagat ttctttgata tgaagggcgt ggtagaggaa
     8701 ttcttcgaca aggtgggaat ggataagaag gttcattacg atccaaaggg cgaccatcct
     8761 tacctgcatc cgggcagaaa ggcagatatc gtttatgagg gccgcaccgt gggcttcttg
     8821 ggtgagatcc atccggaggt ggctgaaaat tataagattg gcgacagagc ctatgtagct
     8881 gtgatcgata tgcccgcaat gacagagttt accacctttg accggaagta caccggaatt
     8941 gccaaattcc cggctgttac cagagatatc agtatggttg ttccaaaggc agttttggta
     9001 gggcagatcg aggatgtgat cagccagaga ggcggaaaga tcttagagag ctgcaagctg
     9061 ttcgatattt atgagggagc acaggtactg gaaggccaca agtccgtggc atactccatt
     9121 actttcagag ctaaggatca cactctggaa gagaaggaag tcacagcggt gatgaataag
     9181 atcttaaatg gccttaagaa tcttggaatt gagctgaggg cgtaaagcaa atagaagatt
     9241 ttttaagagg gcagagctgt tttggctttg ccctttttta actgaaaagg agaatgtgta
     9301 tggatggtaa aagcagaagc tatatgaggg ctgtatgtgc agttctggca gaaggacttt
     9361 tggcggcggg ctgtggaaaa gaagaaacag cggcagcgga aacagcggag atagcagtcc
     9421 cggaagccgc aaaaagaaca gagggttcgg aaactaccgc atctgcagag tttttttcag
     9481 agtttaaaaa tacggtgttt atattttcaa gcggtgcagg tgcatggagg acggagcttc
     9541 agatacggga agacggcagc ctttcgggac gatttgagga tgccaatatg ggagaaatcg
     9601 gagaagagaa tccgcaggga accatatatg agtctgtgtt cacaggccgt cttggatctc
     9661 ctgtccggct ggatgaaaac agctatcagg tggacatttt ggagatggaa tatgaaaatc
     9721 cattgtaaac agaggaaatt atgttgttgt gaattcagtc cattcttatg gttttgtggg
     9781 agaggatctg gcagttcgga tgagggagag ggtagcggga ttggatcaaa gacaaggaaa
     9841 cggatgcaga tgaggccgga aaggagtttg aaggaggaag tatgcagctc atggcataca
     9901 gtttaaaggc aatggagatt accagagaga gggtctatga gctgatggag ctgttggatt
     9961 gatggcttgc aatgttaatt acggagcatg
//
LOCUS       DBMDUH010000098         9851 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_3295_length_9851_cov_7.764718, whole genome shotgun sequence.
ACCESSION   DBMDUH010000098 DBMDUH010000000
VERSION     DBMDUH010000098.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 9851)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9851)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9851
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3295_length_9851_cov_7.764718"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1379)
                     /locus_tag="ACZPCG_08995"
     CDS             complement(<1..1379)
                     /locus_tag="ACZPCG_08995"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HIR1285226.1"
                     /translation="MEMIKTKTANAIRVLSADAIEKAKSGHPGLPLGCASIAYELWAE
                     HMKHNPADPEWVDRDRFVLSGGHGSMLLYSLFHLFGYGDLSIDDIKNFRQTGSLTPGH
                     PEYRHTVGVEATTGPLGQGMGMAVGMAMAEAHLAAVFNKEDCKIVDHYTYVLGGDGCM
                     MEGLSSEAFSLAGTLGLGKLIVIYDSNNISIEGNTDIAFREDVAKRMEAYGFGVFKVE
                     DGNDFDAIGAAIEAAKADTDRPSIIIVHTEIGYGCPAKQGKASAHGEPLGGDNVLALK
                     ENIGWESMEPFFVPQDVYDHMETVKERLKTPEEEWKARFALYCEKYPEMKELWDQYHD
                     GNLAEKLYDQEEFWAFEDKPQATRNVSGELLNRINQAVPNLFGGSADLAPSNKTALKG
                     AGDFSKEDYSGKNLHFGVREQAMAAIGNGLALHGGVIPYVATFFVFSDYMKPVARLSS
                     LMKVPMTYVL"
     gene            complement(1517..2230)
                     /locus_tag="ACZPCG_09000"
     CDS             complement(1517..2230)
                     /locus_tag="ACZPCG_09000"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1285227.1"
                     /translation="MIKAVFFDIDNTLYSYDRGNEAGMKALTVYGETVLQMDRETFVK
                     VFRQAQQLVKERTGADCAAVHNRLIRMQCILELLGKPLFPHATVMYHLYWDSLLSSAR
                     PEEGVLQLMQLLKTKGIGIGIGTDMTAYIQYKKLEVLGVSSYIDWIVTSEEAGAEKPA
                     GRFFDLCAQKAGAAPSQCAFIGDSLEKDVEGALKNGFKGVWYHKNPTEDEKRSYPVID
                     SFCGCIQDDRICLGKEILI"
     gene            complement(2245..2937)
                     /locus_tag="ACZPCG_09005"
     CDS             complement(2245..2937)
                     /locus_tag="ACZPCG_09005"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006862948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HIR1285228.1"
                     /translation="MLEELKKRVYEANMLLPKHGLVTFTWGNVSEIDRETGYFAIKPS
                     GVDYDKLTPDDMVIVDLEGNKIEGKYNPSSDTATHVELYKAFSKIGGIVHTHSPWATS
                     WAQAGRGIPCYGTTHADYMYGEIPCVRCLTKEEIEGAYEKNTGVLIADYFKDKDYEAV
                     PAVLCKNHGPFTWGKDGHEAVHNAVVLEEVAKMAARCEMINPRVQPAPQELQDKHYYR
                     KHGVNAYYGQGK"
     gene            complement(2949..4001)
                     /locus_tag="ACZPCG_09010"
     CDS             complement(2949..4001)
                     /locus_tag="ACZPCG_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285229.1"
                     /translation="MKAVKKVISNLTKCYSIAPLHYQGKDHILVAAEKVDRCLLFDLD
                     GNQEDTVWEQPGGVMTMQQVPGTDGQFLATHKFYSPNDSKDAKIVIVTPKSKGNWEVR
                     TLTDLPFVHRFDILERNGVHYLIACCLKSGHEFKDDWSSPGRVFAAVLPEDLSSFDEE
                     HQLEMKVIKDQMLKNHGYYRVKENGQDTSVVSCENGIYQFIPPASPEGEWEITQLLDT
                     PASDALLLDMDGDGEKELAVLAPFHGEKIDFYKKKDGRFEHVYSYGKDAEFVHAIWGG
                     DIGGVPTVIIGHRKGDRDLLAFTYDQEKQEYRAERLDHDCGPANAYAYKYDGKDILIA
                     TNREIDEVARYELEME"
     gene            complement(4017..4862)
                     /locus_tag="ACZPCG_09015"
     CDS             complement(4017..4862)
                     /locus_tag="ACZPCG_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009267989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HIR1285230.1"
                     /translation="MAKAYTLGLYEKAMPKDLSWKEKMLAAKGAGFDFIEISIDETDE
                     KLARLDMSREERLALMELMSETGVPIRTMCLSGHRKYPLGSNDPETCARGMEIMEKAI
                     RLAEDLGIRIIQLAGYDVYYEEGSEETRERFEENLKKAVEMAAKAGVMMGFETMETEF
                     MNTVEKAMVYVNRVNSPYLNVYPDLGNVTNAAVTYGTDVEEDLETGRGHLAAMHLKET
                     LPGKFREIPFGTGHVDFEAGIRKAWELGVRKFVTEFWYVGNPDWTEDLKFACDKMTKI
                     LQGNE"
     gene            complement(4864..5691)
                     /locus_tag="ACZPCG_09020"
     CDS             complement(4864..5691)
                     /locus_tag="ACZPCG_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HIR1285231.1"
                     /translation="MGGETVKREKVYVDERRERIRDILREKSQVRVEALAEELKVSVI
                     TIRRDLQYLEEQKQLIRVYGGAVAVREQGEQTDEIQLYRDLIARYAASLVEDGDTLFL
                     NTSRNVRQILRYITKKNVTVITNNGRAIDAERGQGVNVILTGGELRYPKEAMVGDYAI
                     RNLQTVIAKKAFVGCSGIDAVMGMTTENANEVTINQLMVRNVAKDAYILADHTKLGQI
                     SSFISCPIAEIHYLITDELAPAEAVEELRAKGVRVHQVKKADFPNEGLINQTEKKGK"
     gene            complement(5807..6112)
                     /locus_tag="ACZPCG_09025"
     CDS             complement(5807..6112)
                     /locus_tag="ACZPCG_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009536646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HIR1285232.1"
                     /translation="MANANNLKFLVCCANGSGSSLMAQLALDKVLKRNGIKCKTHHAP
                     LSEGKSTAAQYDVLLCAQNFASMFTDAEAKGVKVIPLKNVMSDKEIEEKLKAQGIIE"
     gene            complement(6139..7635)
                     /locus_tag="ACZPCG_09030"
     CDS             complement(6139..7635)
                     /locus_tag="ACZPCG_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007705737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HIR1285233.1"
                     /translation="MEILSKIWSYFATNILQQPAFMIGFIVLIGYILLQRPWYDILAG
                     TIKAIVGYLILTVGSGGLVNNFRPVLVGLGERFNINATVIDPYFGQNAVTAGVEEVFG
                     KGFGDAMILLFIAFIVNILLVRFSKYTKLRALFTTGNVQVQQAATAYWLIMFALPGLL
                     APGMHVPMLIVMALLLGAYWAVGSNLTIKPCQEVTDGAGFALAHQQMFGVAFFYWLAG
                     KLFGNEGKKKDKKVKKIEDLELPGFLSIFNDNMVSASVLMLVFFGAILCVLGKDHLIA
                     GGFMKEGQSMLFYVIQTCLYFAVYLAILQMGVRTFVAELTTSFQGIASKLLPGSVPGV
                     DCAVIFGFGASNAVTLGFVSGFIGQILAIIVLIALKSPVLIICGFVPVFFDNATIGVF
                     ANEKGGLKAVLVLPFISGLCQVFGSALIAGWVGMAAYGGYLGMWDWAVVWPVFTVVMK
                     YLSYIGVTLIFIGLLAIPQIQYYKDKKGYFLMTEDYEAYKELKANKAN"
     gene            complement(7663..8118)
                     /locus_tag="ACZPCG_09035"
     CDS             complement(7663..8118)
                     /locus_tag="ACZPCG_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007705733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIR1285234.1"
                     /translation="MLREFVEQKHYKFAAEAPDWKEAIRMSCESLEADGTIEANYKED
                     IVVCVEKYGPYIVLMPNIAMPHSQEGAEGVHKTSIGFMKLDKPVSFDPEDPEKDAQLF
                     FTLASCNPEQHMNNMVRLSELLMNEEVVAALLKAETPEDLLEIQKQYLD"
     gene            complement(8148..9251)
                     /gene="ulaG"
                     /locus_tag="ACZPCG_09040"
     CDS             complement(8148..9251)
                     /gene="ulaG"
                     /locus_tag="ACZPCG_09040"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HIR1285235.1"
                     /translation="MSKIDEITRESWIMGTFPEWGTWLNEEIENEEVKPGTVAMWWLG
                     CTGIWFKTPGGCNLTVDLWAGNGKRTHGDGKMKPGHQMANMCGGRMMQPNLRAVPFVI
                     DPFAIKQVDAVLATHYHQDHMSAEYAAHVIKSGMTTTNEKGEEVPVPFIGPKKSVELW
                     QKWGVPADRCITVKPGDTIKIKDVEIVALDSFDRTCLVTTDDTSENREELTGICPTDM
                     DDKAVNYLIKTPGGNIYHSGDSHYSIYFAKHGKDYDVDVAFGSYGENPVGMADKMTSV
                     DILRMAEALRAKVVIPIHYDVWTNFMADINEIRVLYDMKKDRLDYKFHPFFWEVGGKY
                     VYPTDKDKKAYHHRRGFEDCFEHPQNIPFRSCL"
     gene            complement(9704..>9851)
                     /locus_tag="ACZPCG_09045"
     CDS             complement(9704..>9851)
                     /locus_tag="ACZPCG_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HIR1285236.1"
                     /translation="AATIANSRRKYWYISNNKAVIWALNKERLINSGYYDLATAYQSV
                     HVNY"
BASE COUNT         2446 a         2664 c         1851 g         2890 t
ORIGIN      
        1 agaacgtatg tcataggcac cttcatcagg gaagacagtc ttgccacagg cttcatgtaa
       61 tcgctgaata cgaagaaggt agccacatag ggaatcactc cgccatgaag agccagaccg
      121 tttccgatag ctgccatagc ctgctctctt acgccgaaat gcaggttctt gccgctgtaa
      181 tcctctttgg agaaatctcc cgcacctttt aaagcagttt tattggaggg agccaggtca
      241 gcagaaccgc cgaacaggtt tggaactgcc tgattgatgc ggtttaacag ctctccggaa
      301 acgtttctgg ttgcctgagg cttatcctca aaggcccaga actcttcctg atcgtaaagc
      361 ttttctgcca gatttccgtc atggtactga tcccaaagtt ccttcatttc aggatatttc
      421 tcgcagtaca gggcaaaacg tgccttccac tcttcttccg gagttttcag acgctctttt
      481 actgtttcca tgtgatcgta tacgtcctga ggaacaaaga agggctccat gctctcccag
      541 ccgatgttct cctttaatgc aagcacattg tctccgccca gaggctcgcc gtgggcagaa
      601 gccttgccct gctttgcagg acagccatag ccgatctcgg tatgaacaat gattattgac
      661 ggtctgtctg tatctgcctt ggctgcctca atggctgcac cgatggcgtc aaagtcattt
      721 ccatcctcta ccttaaatac gccgaagcca tatgcttcca tacgctttgc cacatcttcc
      781 ctgaacgcaa tgtctgtatt tccctcaatg gaaatattgt tggaatcata gattacgatc
      841 agctttccaa gtcccagagt acctgccaga gagaatgctt cagaggaaag tccctccatc
      901 atacagccgt ctccgcccag tacataggta taatggtcta cgattttgca gtcttctttg
      961 ttaaatacag cagccagatg agcttcagcc atagccatgc ccactgccat gcccataccc
     1021 tgtcccagag gaccggtagt tgcttctacg cctacggtat gtctgtactc cggatgtccc
     1081 ggtgtcagag aaccagtctg acggaagttc ttaatatcat caatggagag atctccgtat
     1141 ccgaacagat ggaacagaga gtacaacagc atagagccat ggcctcctga taatacaaaa
     1201 cggtctctgt caacccattc agggtcagcc ggattgtgtt tcatatgctc tgcccacagc
     1261 tcgtaggcaa tagaggcgca gcctaaagga aggcccggat ggccggactt tgccttttca
     1321 atggcgtccg cagaaagtac gcggatggca ttggcggttt tagtcttgat catttccatg
     1381 atcgttctcc tttctcttgt gtcatgtctt tttgtttctg ctataaacca gtattacact
     1441 taatcttatc atttcatttt gattccgtct agggtgagtt ttgaatttat ggcgtttacc
     1501 gccggcatct gtgcctttaa atcaaaattt ctttacccag gcatatcctg tcatcctgta
     1561 tgcatccgca gaatgaatca atcaccggat atgatcgttt ctcatcttcc gtaggatttt
     1621 tatgatacca tacgccctta aatccgttct ttaaagcacc ctctacgtcc ttttcaagac
     1681 tgtctccgat aaaagcgcac tgagacgggg ctgctcctgc cttttgcgcg cacagatcaa
     1741 agaaacgtcc ggccggtttt tccgctccag cctcctcgct ggttacgatc cagtcaatat
     1801 aagaagaaac tcccagcact tccagttttt tatactggat atacgccgtc atatccgtgc
     1861 ctatcccaat tccgatccct ttcgttttaa gcagctgcat caactgcaga accccctctt
     1921 caggacgggc agaagataaa agactgtccc aatataaatg atacatgact gttgcatggg
     1981 gaaaaagcgg ctttcccaat aattccaaaa tacactgcat ccggatcaga cggttatgca
     2041 ctgctgcaca gtctgcgcct gtccgttcct ttaccagctg ctgtgcctgg cgaaacacct
     2101 tcacaaatgt ctcccgatcc atctgaagaa ccgtttctcc gtaaacagtc agggctttca
     2161 ttccagcctc atttcctctg tcatagctgt aaagagtgtt gtcaatatca aaaaataccg
     2221 cttttatcat ctgattctcc cgtattattt cccctgtcca tagtaagcat taacgccatg
     2281 ttttctgtaa tagtgcttat cctgaagctc ctgaggcgca ggctgcaccc ggggattgat
     2341 catctcacag cgggccgcca ttttggcaac ctcctccagc acaactgcat tgtgaaccgc
     2401 ctcatgtccg tcctttcccc aggtaaaggg accgtgattc ttacataaaa cagccggaac
     2461 cgcttcataa tctttatctt taaaataatc tgcgatcaga acccctgtat tcttctcata
     2521 agcaccctcg atctcctcct ttgtcaggca tctgacacag ggaatctctc cgtacatata
     2581 atccgcatgg gtagttccgt agcacggaat ccctctgccc gcctgagccc agctggtcgc
     2641 ccagggtgaa tgtgtatgta caattccgcc gatctttgaa aatgccttat acagctccac
     2701 atgggtagct gtatcagaag aaggattata ttttccctcg attttatttc cttccagatc
     2761 cacgattacc atatcatccg gagtcagctt atcataatcc acgccgctgg gcttaatagc
     2821 aaaatatcct gtttcccggt caatctcgct tacatttccc caggtaaacg ttacaaggcc
     2881 atgctttggc agcagcatat ttgcctcata aactcttttt ttcagttctt ctagcataat
     2941 gtcctccctt attccatttc cagctcgtat cttgccactt cgtcaatctc acgattcgtt
     3001 gcgatcagaa tgtcttttcc atcatattta tacgcatatg cattggcagg gccgcagtca
     3061 tggtcaagcc gctctgccct gtattcctgc ttttcctggt cgtaggtaaa tgccagcaga
     3121 tcccggtctc cctttctgtg gccgataatt acagtgggaa caccgccgat atcgccgccc
     3181 cagatggcat gaacaaattc cgcgtccttc ccatagctgt atacatgctc aaagcggccg
     3241 tctttctttt tgtaaaagtc gatcttctcg ccatggaagg gcgccagaac tgccagctcc
     3301 ttctctccgt ctccgtccat atcaagcagc agcgcatcgc tggccggggt atcaagaagc
     3361 tgtgtaatct cccactcgcc ctcaggtgat gcaggcggaa taaactgata gattccgttt
     3421 tcacaggaaa ccacgcttgt atcctgtccg ttttccttta cacggtagta gccgtggttt
     3481 ttgagcatct gatccttgat caccttcatc tccagctgat gctcctcgtc aaagctgctt
     3541 aaatcctccg gaagcacagc cgcaaacaca cggccgggag aggaccagtc atccttaaac
     3601 tcatggccgg attttaaaca gcaggcgatc agatagtgta ccccatttct ctctaaaatg
     3661 tcaaaccggt gtacaaaggg cagatcggtc agagtgcgta cctcccagtt tccctttgat
     3721 tttggagtca caattacgat ctttgcgtcc tttgagtcat tgggagaata aaatttatgg
     3781 gtagcaagaa actggccgtc cgttcccgga acctgctgca tggtcataac accgcccggc
     3841 tgctcccaga cagtatcctc ctggtttcca tccagatcaa agagcagaca gcggtctacc
     3901 ttctcagctg ccaccaggat atgatccttt ccctgataat gaagaggtgc tatggaataa
     3961 cattttgtca gattggaaat tacttttttt actgccttca tcgtttaacc gcctttctac
     4021 tcattaccct gtaaaatctt cgtcatctta tcacaggcaa acttcagatc ttctgtccag
     4081 tctggatttc ctacatacca gaactcggtc acaaacttcc gaacgcccag ctcccacgcc
     4141 ttgcggatac cggcctcaaa gtctacatgg cctgtcccaa aaggaatttc gcggaattta
     4201 cccggaagtg tctcttttaa atgcatggca gccagatggc cccgtccggt ttccagatcc
     4261 tcttccacat ctgtaccata cgttacagcc gcattggtaa catttcccag atcaggatat
     4321 acattcaaat acggagaatt tacccgattt acataaacca tcgctttctc taccgtattc
     4381 ataaattccg tttccatggt ctcaaagccc atcatcactc cggcttttgc cgccatctcc
     4441 actgcttttt ttaaattttc ctcaaaacgc tctcttgtct cttcactgcc ttcttcataa
     4501 taaacatcat aaccggccag ctgaatgatc cgaatcccca gatcctctgc cagacggatc
     4561 gccttttcca taatctccat tcctctggcg caggtctctg gatcattgct tcccaaaggg
     4621 tattttctgt gtccactcag acacatggta cggatgggaa cacccgtctc actcatcagt
     4681 tccattaaag ccagccgttc ttccctgctc atatcaagcc gtgccagctt ttcatctgtt
     4741 tcatcaatac taatctctat aaaatcaaat cctgcccctt ttgccgccag catcttttcc
     4801 ttccagctca gatcctttgg catggccttc tcataaaggc ccagtgtata tgcttttgcc
     4861 attttatttt cctttctttt ctgtctgatt tatcagtcct tcattcggaa aatctgcttt
     4921 cttcacctga tgaactctta ctccttttgc cctcagctcc tccacagcct ctgccggagc
     4981 cagttcatcc gtaatcagat agtgaatctc cgcaatagga cagctgataa agctactgat
     5041 ctgccccagc ttggtatggt ctgccagaat atatgcatcc tttgccacat tccgcaccat
     5101 tagctggtta atcgtaacct cattggcgtt ttccgttgtc attcccataa cagcatcaat
     5161 gccggaacat cccacaaagg cctttttggc aattaccgtc tggagattgc ggattgcata
     5221 atcccccacc attgcctctt ttggataacg cagctctcct cctgtaagga ttacatttac
     5281 cccctggcct ctctctgcat caatcgctct tccattattt gtgatcactg taacattttt
     5341 ttttgtaata tatcgaagaa tctggcggac attccggctg gtattaagaa acagtgtatc
     5401 gccgtcctct accagactgg ccgcatagcg cgcaatcaaa tcccggtaca gctggatttc
     5461 gtccgtctgc tccccttgtt cccggactgc aactgcacct ccgtataccc ggatcagctg
     5521 cttctgctct tccagatact gtaaatccct gcgtatcgta atcacagaaa ccttcagctc
     5581 ttctgccagc gcctctaccc gaacctgaga tttttccctt aatatatccc ggatccgttc
     5641 tctccgctca tccacatata ccttctcacg cttcactgtt tcccctccca ttcttttaaa
     5701 ataaggcaaa tatttaacag tccttccgcc gttgtgataa taaaggctgc agcagtcagc
     5761 tgcggcagcc tttttaacaa gatctgctgt ttatacgctc actgtactat tcaataatac
     5821 cctgagcctt cagcttctcc tcaatctctt tatctgacat aacattcttt aacggaatca
     5881 cctttacacc ctttgcctct gcatctgtga acatactggc aaagttctgg gcacacagca
     5941 gaacatcata ctgtgcagct gtgctcttcc cctcagacag cggcgcgtga tgggttttac
     6001 acttaatgcc gtttcttttt aacaccttgt ctaatgccag ctgagccatc aggctggaac
     6061 cggaaccatt tgcgcagcac acgagaaatt ttaaattatt tgcatttgcc atgaatatat
     6121 cctccatata aaattatttt agtttgcttt gtttgccttt aattccttat atgcctcata
     6181 atcttctgtc ataaggaagt aacccttctt atccttgtaa tactggatct gcggaatagc
     6241 aagaagaccg ataaaaataa gagttacgcc gatatagctt aaatacttca taactactgt
     6301 aaatacaggc catacaaccg cccagtccca catacctaaa tatccgccgt aagctgccat
     6361 tccaacccag cctgcaatca gagcagaacc gaatacctgg cataatccgg agataaacgg
     6421 aagaaccaga acagccttta agccaccctt ttcattggca aatacaccga tggtagcatt
     6481 gtcaaagaat acaggtacga aaccgcagat aataagtacc ggactcttta aagcaatcag
     6541 aacaataatt gccaggatct gtccgataaa accggataca aagcccagag tcactgcatt
     6601 ggatgcaccg aaaccaaaga taactgcaca gtctacacca ggaacagaac ccggaagcag
     6661 cttgcttgca ataccttgga aagaagttgt cagctcagca acgaaggtac ggacgcccat
     6721 ctgcagaatt gccagatata ctgcaaagta aagacaggtc tggattacat agaacagcat
     6781 gctctgccct tccttcataa aaccgcctgc aatcagatgg tcctttccaa gtacacataa
     6841 gattgcgcca aagaatacca gcatcaaaac agaagcagat accatgttgt cgttgaaaat
     6901 agataaaaag ccgggaagtt ccagatcctc aatcttctta accttcttat ctttcttctt
     6961 gccttcattt ccaaaaagct ttcctgccag ccaatagaag aaagctacac caaacatctg
     7021 ctgatgagcc agagcaaagc ctgctccgtc agttacttcc tgacatggtt taattgtcag
     7081 gttagaacct actgcccagt aagcacccag aagaagagcc ataacgatca gcatcggaac
     7141 atgcatgccg ggagccagaa ggcccggaag agcaaacatg atcagccagt aagcagttgc
     7201 agcctgctgt acctgtacgt ttcctgtggt aaacagtgcg cgaagctttg tatatttgct
     7261 gaagcgcacc agcaggatat ttacaataaa tgcgataaac agaaggatca tcgcatcgcc
     7321 gaagcctttt ccgaatactt cttctactcc ggctgtaacg gcattctgtc caaaatacgg
     7381 atcaataact gttgcgttaa tattgaagcg ttcacccaga cctaccagta caggacggaa
     7441 gttgttaacc agtccgccgg aaccaacagt cagaatcagg tatcctacaa ttgctttaat
     7501 tgtgcctgcc agaatatcat accatggtct ctgaagcaga atataaccga tcaatacaat
     7561 aaaaccaatc ataaatgccg gctgctgcag gatatttgtt gcaaaatatg accagatctt
     7621 agacaaaatt tccataagtc accccttatt ccctctctta aatcaatcca gatattgttt
     7681 ctggatttcc aacagatctt ccggtgtttc agcctttaat aaggctgcca ccacttcttc
     7741 gttcatcaga agttctgaca gacgaaccat attgttcatg tgctgctccg gattgcagga
     7801 agccagcgta aaaaacagct gtgcgtcctt ttccggatcc tccggatcaa aagaaaccgg
     7861 cttgtccagc ttcataaaac caatgctggt cttatgtacc ccttcagccc cctcctgaga
     7921 atggggcatg gcaatgtttg gcataagcac aatatatgga ccatatttct ccacgcagac
     7981 aacaatatct tccttataat ttgcctctat cgtaccgtct gcctccagac tttcgcagct
     8041 catgcggatg gcttccttcc agtccggcgc ttcagctgca aacttataat gtttctgctc
     8101 tacaaattct ctcagcatat aactttcccc ctaacactta tctgacctta caggcaggaa
     8161 cggaatggaa tattctgagg atgttcgaag cagtcctcaa atccgcgtct gtgatggtaa
     8221 gctttcttat ccttatctgt aggatataca tattttcctc ccacttccca gaagaatgga
     8281 tggaacttgt agtccagacg gtctttcttc atatcataaa gtacccggat ttcattgata
     8341 tctgccataa agttggtcca tacgtcatag tggatcggga ttacaacctt ggctctcaat
     8401 gcctctgcca tacggagaat atctacagag gtcatcttgt ctgccatacc cacaggattc
     8461 tcaccatagg aaccaaaagc cacatctaca tcataatctt tgccgtgctt tgcaaagtaa
     8521 atggagtagt gggaatcacc gctgtgatag atatttccgc ccggagtctt aatcagatag
     8581 tttactgcct tgtcatccat atcggtagga caaattcctg taagctcttc tctgttctcg
     8641 ctggtatcat ctgttgttac caggcaggtt ctgtcaaagg aatccagagc aacgatctca
     8701 acgtctttga tcttaatcgt atcacccggt tttactgtaa tgcagcgatc tgcaggaaca
     8761 ccccatttct gccacagttc tacagatttc ttcggtccga taaatggtac gggaacctct
     8821 tctccctttt cgtttgttgt tgtcatgccg ctcttaatta catgggctgc atactcagcg
     8881 ctcatatgat cctgatggta atgggtcgcc agcactgcgt ctacctgctt aatggcaaag
     8941 ggatcgatca caaaggggac tgctcttaag ttaggctgca tcattctgcc gccgcacata
     9001 tttgccatct gatgtcccgg cttcatcttt ccgtctccat gggttctctt tccgtttccg
     9061 gcccaaagat ctacggtaag attgcatcct ccaggagtct taaaccagat accggtacat
     9121 cccagccacc acatggcaac agttcccggc ttcacctcct cgttctcaat ctcctcattc
     9181 agccaggttc cccactcggg aaatgtcccc atgatccagc tctctcttgt gatctcatca
     9241 atcttgctca tttcgcatcc tccttgtttt tgatcatttt tatgattgtt cttatgcgtt
     9301 tatctctgtc tttacttgaa tattagcact ttttgtgcag tatggcaatg gtattatatg
     9361 attgtttcat attttatatg attcatttat tattttgcat tatattacac gtaatttaac
     9421 ttatctttta tatattttat ccatcatttt taccattcca ttcgttttat ctttgtattt
     9481 taatttaatt ttttatatat ttacttatta tcctgatttt tacatacttt ttcctttgtt
     9541 atatgattct tttttatgga tttcggtctt atcttttagt cgtcatccct tcatatgatt
     9601 ctttttacga tatcagcgta ttcacctttt cgatcgagta ggaggcggtg attaaccgcc
     9661 gtcctctcac agcaccgtac gtaccgttcg gtatacggcg ctttcaatag ttgacgtgca
     9721 cagactggta ggctgtggct aaatcataat agccactgtt tatcagtctt tctttattca
     9781 gcgcccaaat cacagctttg ttattactga tataccaata ctttctgcga ctgtttgcta
     9841 ttgttgccgc a
//
LOCUS       DBMDUH010000099         9737 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_3331_length_9737_cov_8.440120, whole genome shotgun sequence.
ACCESSION   DBMDUH010000099 DBMDUH010000000
VERSION     DBMDUH010000099.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 9737)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9737)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9737
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3331_length_9737_cov_8.440120"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1320)
                     /gene="rlmD"
                     /locus_tag="ACZPCG_09050"
     CDS             complement(<1..1320)
                     /gene="rlmD"
                     /locus_tag="ACZPCG_09050"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HIR1285237.1"
                     /translation="MEKNQEFTVTIEDMSEDGSGIGKLDGYIWFIKDTVIGDQVRAKA
                     MKMKKSYGFARLMEVITPSAFRTEPRCPAARPCGGCQLQAMKYEEQLAFKERKIKNNL
                     MRIGGFKEEEIPMFPIIGMEEPWRYRNKAQFPFGMNKNGEIITGFYAGRTHDIIENAD
                     CLLGVQENRGILEIIKAFMKEYRIRPYDEKSHQGLVRHALIRKGFKSSEIMVCLVING
                     KRLPHGEELVKRLSAVSGMTSISYSINEEKTNVIMGKEIVNLYGPGYITDSISDVTYQ
                     ISPLSFYQVNPVQTEKLYGTALEYAGLTGKETVWDLYCGIGTISLFLAQKAKKVYGVE
                     IVPQAIEDARRNARLNGMENVEFFVGKAEEVLPEQYEKNQIYADVIVVDPPRKGCDQM
                     CLDTILKMRPERVVYVSCDSATLARDLRYLVDGGYEVKKVRGCDMFPQ"
     gene            complement(1326..2270)
                     /locus_tag="ACZPCG_09055"
     CDS             complement(1326..2270)
                     /locus_tag="ACZPCG_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HIR1285238.1"
                     /translation="MRYIETFHEGNHISDIYLCKTKQIQLTKTGKEYGNLILQDKTGT
                     IEAKIWDLSSPGVGEFDAMDYVHIEADVTLFQNANQLNVRRIRTAREGEYVEADYLPV
                     SKKEIGKMYEELLGFVRSVKNPWLNQLLSGYFVEDKEFAKAFQFHSAAKTVHHGFVGG
                     LLEHTLSVVKLCDYYAGYYKTLNRDLLLTAAMFHDIGKMQELSRFPENDYTDDGQLLG
                     HIMIGTEMISERIRQIPDFPPRLASELKHCILAHHGELEYGSPKKPALLEALALNFAD
                     NTDAKMETMIEALAAGGTNKGWLGYNRLLETNIRKTTE"
     gene            complement(2335..3657)
                     /locus_tag="ACZPCG_09060"
     CDS             complement(2335..3657)
                     /locus_tag="ACZPCG_09060"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR1285239.1"
                     /translation="MADKREDKGTDEGQEKQKPFIREKIVRQPPSGRHIMRKILLTGV
                     CAVVFGTAAAVCFSAVKPIADRAFGLEETEQEPITIAKDEPEAQQPVQTECAPVPEET
                     EPVEDMVRSEMEKYPYSMDDLNRLYGNLNTLVEENEGCVASVHSIQRETDWFDNPIET
                     TGQYSGVVISCTRGEILVLVPDEAVDTADSIEVSFSGGTILPGTVKQKDSVADMALVS
                     VNAAELEDRQYERIKELPLGNSYGVRQGDLVVAIGSPAGVTGSSGYGAISYVVRSTKA
                     VDGSTRIFYTDTAADSQAGTFLINTDGELIGWAVDDYGQEDHSSMTAVMSVSDYKGAL
                     ELMSNGLPVPYFGILGQEITAAMAQKGMPKGIYISEAVMDSPAYNAGLQPGDILTKLG
                     DMPVTNLKEFQGQVEKLQAGTKIIVNVQRSNGKDEYREIPFEITVGTR"
     gene            3828..6335
                     /locus_tag="ACZPCG_09065"
     CDS             3828..6335
                     /locus_tag="ACZPCG_09065"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0045910 - negative regulation of DNA
                     recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HIR1285240.1"
                     /translation="MNQKALKTLEYTKIITQLESHAASPLGKSLCQELSPSSDLEEIR
                     TRQAQTTDAVNRVRLKGSVSFSGLREIDGSLKRLEIGSSLSIPELLSISSVLTVASRA
                     KAYGRRDTEDPPVFTPRFPGQKPPKQAEVAEYVPDSLDPLFQSIEPLTPLNNEIKRCI
                     LSEDEIADEASPGLSRVRRSIKAAADRIHTQLNSILNSHRTYLQDAVITMRDGRYCLP
                     VKAEYKSQVSGMVHDQSATGSTLFIEPMAIVKLNNEIRELEIQEQKEIEAVLATLSNE
                     AAPHIEELRLNMELLAQLDFIFAKAALSRQYQCSAPVFNDKGRIHIKDGRHPLLDPQK
                     VVPINIWLGKDFDLLIVTGPNTGGKTVSLKTVGLFTLMGQAGLHIPAWEGSELAVFDE
                     VFADIGDEQSIEQSLSTFSAHMTNIVSILQQADSRSLCLFDELGAGTDPTEGAALAIA
                     VLSFLHNMKCRTMATTHYSELKVFALTTPGVENACCEFSVETLQPTYRLLIGIPGKSN
                     AFAISKKLGLPDFIIEDAKSHLEAKDESFEDLLASLETSRVTIEKEQEEIRSYKEEIA
                     QLKSRLTQKEERLDERKDKLIRNASEEAQRILREAKETADQTIREINRLASESGVGKE
                     LEAQRAKLREQIKKTDDKLAVKAKGPSQPISPKKLKIGDGVKVLSMNLKGTVSTLPDA
                     KGDLFVQMGILRSRVNIRDLELIREDDISATLGDGSSRTYGGTAAGSKAKKTFSQAKG
                     NGSGSGQIRMSKSFSVGTEVNLIGMTTDEAVPAMEKYLDDAYLAHMPSVRVVHGRGTG
                     ALKNACHKRLRQLKYVKDFRLGEFGEGGTGVTIVTFK"
     gene            6359..7069
                     /locus_tag="ACZPCG_09070"
     CDS             6359..7069
                     /locus_tag="ACZPCG_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR1285241.1"
                     /translation="MAEKQKILIVDDDANIAELISLYLMKECYETMIVGDGEEALKVF
                     PEFKPNLILLDLMLPGIDGYQVCRELRTSSQVPIIMLSAKGEIFDKVLGLELGADDYM
                     IKPFDSKELVARVKAVLRRYQAAPASISTEPKQQGEYVEYPDLIVNLTNYSVLYKGHS
                     VEMPPKELELLYFLASSPNQVFTREQLLDHIWGYEYIGDTRTVDVHIKRLREKIKDHA
                     NWSITTVWGIGYKFEVKR"
     gene            7074..8480
                     /locus_tag="ACZPCG_09075"
     CDS             7074..8480
                     /locus_tag="ACZPCG_09075"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1285242.1"
                     /translation="MTRSLYSKFILGYLIFGLLGFITIATFSSRMTRDYLMRERSEAL
                     YDEANDIAASCSQMYDGKRPDLAAFSSQLKSLGAYLRAEIWVADNQGAIFMDSRDGSR
                     TQTVIPDFDPTASGSRSYTIGNYYGLFNEDVLTVSAPVIGNYTTYGYVILHLPVSQIA
                     HSQSEILDILYITSAAIFGLSLIILLVFTQTVYLPLRKITVGAKEYAAGHLDYRIQVK
                     THDEMGYLSDTLNYMSDELDKMEEYQRNFIANVSHDFRSPLTSIKGYLEAILDGTIPP
                     ELYEKYISRVISETERLHKLTESMLTLNSLDARGYLTRTNFDINRVIKDTAASFEGTC
                     GKKNISFDLTFSDNIQMVYADMEKIQQVMYNLIDNAIKFSHNDSVIYIQAAGQYEKVF
                     VSVKDTGIGIPKDSLKKIWERFYKTDLSRGKDKKGTGLGLSIVKEILQSHGENIDVIS
                     TEGVGTEFIFSLPRSTVL"
     gene            8581..9591
                     /locus_tag="ACZPCG_09080"
     CDS             8581..9591
                     /locus_tag="ACZPCG_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003524927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285243.1"
                     /translation="MTRTKQAAAFFMTAALLSSAFAPAALADNKTKVGKICLTVDSDI
                     RAGSSGGEVDVTPTGDNTDLYYVDAVEVVNDDGDDWSKSNPPEVDITLGLEDEEEYAF
                     SGTSSSSFKLTLDSSIKSRFDKIKFVDARKTDGGATVILTVKLVFDEDADMSDAPAPG
                     NLSWDSDNPKVAVWDDVESAKYFQVQLYRNGSLVTPPDGGNSTLSVYNTFYDFTDWMT
                     ENGTYTFKVRSVKASNNAKSNWKTSPEQTAGNSVSEGWKQAADHVRWWWQNADGTYPA
                     SQWKDVNGQWYYFDADGYMATGWIQVNGLYYYLDPSSGAMYANCYTPDNCWVDANGVW
                     IP"
BASE COUNT         2482 a         2626 c         2175 g         2454 t
ORIGIN      
        1 ctgcggaaac atatcacacc ctctaacctt cttcacctca taccccccat ccacaaggta
       61 tctcagatct cttgccaggg tagcggagtc acagctgaca taaacgaccc gttcgggccg
      121 cattttcagg atcgtatcca ggcacatctg gtcgcagccc tttcttgggg gatctaccac
      181 gatcacatcg gcatagatct ggttcttttc atactgctcc ggcagcacct cctctgcctt
      241 tcctacaaag aactccacat tttccattcc attcaaccgg gcatttcttc tggcatcttc
      301 aatggcctgg gggacaatct ccacgccata tacctttttc gccttctggg ccaggaacag
      361 ggaaatagtt ccaatgccgc agtacagatc ccataccgtt tcttttcccg taaggcccgc
      421 atattccagg gctgttccat acagcttttc tgtctgcacc ggattaacct gataaaaaga
      481 caggggggag atctgatatg ttacatccga aatggaatct gtaatatacc ctggaccata
      541 aagatttaca atctcttttc ccatgatcac attggtcttt tcctcattga tactgtaaga
      601 aatgctggtc attcctgaaa cagccgacag ccgctttacc agctcttccc cgtggggcag
      661 gcgctttccg ttgatcacca gacagaccat gatctcactg ctcttaaacc cttttcggat
      721 cagggcatgg cgaaccagcc cctgatggct tttctcatca tagggcctga tcctatattc
      781 cttcataaat gccttgataa tttccagtat tcccctgttt tcctgtaccc ccagcaggca
      841 gtccgcattt tcaatgatat catgggtgcg gcccgcataa aatccggtta taatttctcc
      901 gtttttattc attccaaaag gaaactgagc cttgttgcgg taccgccagg gctcctccat
      961 tccaataatt ggaaacatgg ggatttcttc ctctttaaaa ccgccgatcc gcataaggtt
     1021 atttttaatc tttcgctctt taaacgccag ctgctcctca tatttcatgg cctggagctg
     1081 gcagcccccg cagggacggg ccgcagggca tcttggctct gtccgaaaag cagaaggagt
     1141 gatcacctcc atcagacggg caaaaccata ggattttttc atcttcatgg ctttcgcccg
     1201 cacctggtcg ccgatgaccg tatctttgat aaaccagatg tagccgtcaa gctttccgat
     1261 accggagccg tcctcgctca tatcttcaat ggtaactgta aattcctgat ttttttccac
     1321 tttccttact ccgttgtctt tctgatgttg gtttctaaaa gcctgttgta gcccagccag
     1381 cccttattcg ttccgcctgc tgccagagcc tcgatcattg tctccatctt ggcatctgta
     1441 ttgtctgcaa aattcagtgc cagcgcttcc aaaagagccg gtttcttggg tgaaccatat
     1501 tccagctctc cgtgatgggc cagaatacag tgttttaact cagacgccag tctgggcgga
     1561 aaatccggaa tctggcggat ccgctcggaa atcatctccg tgccgatcat aatatggccc
     1621 agaagctggc cgtcgtcggt ataatcattt tcagggaaac gtgacagctc ctgcatcttt
     1681 ccgatatcgt ggaacatggc tgcggtgaga agcaggtccc tgtttaatgt cttataatac
     1741 cctgcatagt agtcgcagag ctttaccaca cttaaggtat gctctaagag tcctcctacg
     1801 aatccatgat gaacggtctt tgccgcggaa tggaactgga acgccttggc aaattcctta
     1861 tcttccacaa aataccctga caaaagctga ttcagccatg gattcttcac actgcgcaca
     1921 aatcccagca gctcctcata catctttcca atctcttttt tagaaacagg cagataatcc
     1981 gcctccacat actctccctc tctggctgtg cggatccggc gcacattcag ctggtttgca
     2041 ttctgaaaca gcgtcacatc tgcctcaatg tgcacatagt ccattgcgtc aaattcccct
     2101 actcccggag aagaaagatc ccagatcttt gcctcaatgg tgcctgtctt atcctgaagg
     2161 atcagatttc catattcttt tcctgttttt gtaagctgaa tctgttttgt cttgcataaa
     2221 taaatatcgg aaatatgatt tccttcatgg aatgtctcaa tatatctcat tttcatacct
     2281 catctctctt ttactgaaaa gggagtccgc ttcgttcgga ctcccacacc gcacttatct
     2341 ggttccaacg gttatctcaa aggggatttc cctgtattcg tctttgccat tgctgcgctg
     2401 tacgtttaca atgatcttcg ttcctgcctg gagcttttcc acctggccct gaaattcctt
     2461 cagatttgtc accggcatat ctcccagctt tgtgagaata tcccccggct gaaggcctgc
     2521 attataagcc ggactatcca taacggcttc tgaaatatag atgcccttag gcattccctt
     2581 ctgggccatg gctgccgtga tctcctgtcc cagtatacca aagtaaggca caggaagacc
     2641 gttgctcata agttccaagg cccccttata atccgatacg gacataacgg ctgtcatgga
     2701 agaatgatcc tcctgcccat aatcatccac cgcccagccg atcagttctc catccgtatt
     2761 aataaggaag gttcccgcct gggaatctgc cgctgtatcc gtataaaaga tcctggtact
     2821 tccgtctacc gcttttgtgc tgcgcactac ataggaaata gcgccgtatc cactggaacc
     2881 ggtcacaccg gccggggaac cgatggcaac caccagatct ccctgcctga caccgtaaga
     2941 atttcccagc ggaagctctt ttatgcgctc atactgcctg tcctccagct ctgcggcatt
     3001 tacgcttaca agagccatat ctgccacaga atccttttgt tttacagtac ccggaagaat
     3061 ggttccgcct gaaaatgata cttcaatgga atccgccgta tctaccgcct catccggcac
     3121 cagaacaagg atctctcctc ttgtgcagga aatcaccacg cctgaatact ggcctgtagt
     3181 ttcaatggga ttatcaaacc agtccgtttc cctctgaatc gagtgaacag aagccacaca
     3241 gccctcattc tcttcaacca gagtattcaa atttccgtag agacggttca gatcatccat
     3301 agaatacgga tacttctcca tctctgaccg caccatatcc tccaccggct ctgtttcctc
     3361 aggcaccggc gcacactctg tctgtacagg ctgctgcgct tccggctcat cctttgcaat
     3421 ggtgatcggc tcctgctctg tctcttccag gccaaaggct ctgtctgcga tcggctttac
     3481 agccgaaaag cataccgcag ctgctgttcc gaacacaact gcgcagactc ctgtcagcaa
     3541 aatttttctc atgatatgcc gcccggaagg tggctggcgg acgattttct ccctgataaa
     3601 cggtttctgt ttttcctggc cttcgtccgt tcccttgtct tcacgcttat ccgccatttc
     3661 gctcctcctt acgaagaatc tcacctctta ttatacggtt ctcacgctta atatgcaagt
     3721 ttttgttcgt tcggaatttt cccctatcgt aaatatcggg ggatgtggta tacttgtccg
     3781 tgtaaacagg atattttgta ccactctcag gaggttattg ataaaaaatg aaccaaaaag
     3841 cattaaaaac cctggaatat acgaagataa tcacccaact ggaaagccat gcggcctctc
     3901 ctctagggaa gtccctgtgc caggaactgt ccccttcttc tgatctggaa gagatccgca
     3961 cccgccaggc ccagaccaca gacgctgtga accgtgtgcg tttaaagggc tctgtttcct
     4021 tttccggcct tcgggaaatc gacggttcct taaagcgcct ggaaatcgga agctctctta
     4081 gcatccctga gcttctctct atcagctcgg ttcttaccgt ggcttcaaga gccaaggctt
     4141 acggacgcag agatacagag gacccccccg ttttcacccc ccgttttccc ggacagaagc
     4201 cgccgaagca ggctgaagtg gctgaatatg tgcctgactc cctggatccc ttatttcagt
     4261 ccatcgagcc tttaacccct ttaaataatg aaatcaaacg ctgtatcctc tcagaagatg
     4321 aaattgcaga tgaagccagc cccggtcttt ccagggtgcg ccgttccatc aaggctgcgg
     4381 cggaccggat ccacacccag ttaaattcta ttttaaattc ccaccgcacc tatcttcagg
     4441 atgcggtgat caccatgagg gacggacgct actgccttcc tgtaaaggct gaatataaga
     4501 gccaggtttc cggtatggtt cacgatcagt ctgccaccgg ctctaccctt ttcatcgaac
     4561 ccatggctat cgtgaagctt aacaatgaaa tccgggagct ggaaattcag gagcagaagg
     4621 aaatcgaggc ggttttagcc actttaagca atgaggcggc tccccatata gaagagctgc
     4681 gcctgaatat ggagcttttg gcccagcttg actttatctt tgccaaagcc gccctgtccc
     4741 gccagtacca gtgcagtgcg cctgtattta atgacaaagg ccgcattcac atcaaagacg
     4801 gacgccaccc gctcttagat ccccaaaaag tagtccctat caacatctgg ctgggaaagg
     4861 attttgatct tttgatcgtc accggcccca ataccggagg caagaccgtt tccttaaaaa
     4921 ctgtgggact gtttaccctg atgggacagg ccggccttca tatccctgca tgggagggtt
     4981 cggagctggc tgtttttgat gaagtatttg cagatatcgg ggacgaacag agcattgagc
     5041 agagcctcag taccttctcc gcccacatga caaatatcgt atctatttta cagcaggcag
     5101 actcccgttc cttatgcctc tttgacgagc ttggcgcagg aacagatccc accgagggcg
     5161 cggctcttgc cattgctgtg ctgtccttcc tccacaacat gaagtgccgc accatggcaa
     5221 ctacccacta cagcgaatta aaggtctttg ccctcaccac tcccggtgtg gaaaatgcct
     5281 gctgcgaatt cagcgtggaa acgctccagc ccacctaccg cctgttaatc ggcattcccg
     5341 gcaaaagtaa cgcctttgcc atttccaaaa agctgggcct tcccgatttc attatcgaag
     5401 atgcaaaaag ccatctggaa gccaaggacg aatcctttga agatcttctt gccagcctgg
     5461 aaaccagccg cgtcaccatt gaaaaggagc aggaggaaat ccgctcctat aaggaggaga
     5521 ttgcccagct caaatcccgc ctgacccaga aggaagaacg tctggacgag cgcaaggata
     5581 agctgatccg caatgcctct gaggaagccc agaggatttt aagagaagcc aaggagacgg
     5641 cagaccagac catccgcgag atcaaccgcc tggcttctga atccggcgtc ggaaaagaac
     5701 tggaggctca gagagccaaa ctgcgggagc agattaaaaa gaccgacgat aagctggcag
     5761 taaaggccaa aggccccagc cagcccattt ccccaaagaa actaaaaatc ggagacggcg
     5821 taaaggtgct ttccatgaac ctgaagggca ccgtcagcac cctgccggat gcaaagggcg
     5881 atctctttgt ccagatgggc attctccgtt ccagagtcaa tatccgtgat ctggagctga
     5941 tccgtgaaga cgacataagc gccacccttg gagacggttc ctcacgcacc tacggcggca
     6001 cggcggcagg ctccaaggca aagaaaacat tttcacaggc aaagggaaat ggcagcggaa
     6061 gtggccagat ccggatgtca aaatcatttt ccgtgggaac agaggtcaat ctcatcggca
     6121 tgactacaga cgaggccgtg cctgccatgg aaaaatatct ggacgatgca tatctggccc
     6181 atatgccgag tgtgcgggtt gttcacggac gcggtacggg agcgctgaaa aatgcctgtc
     6241 acaagcggct gcgtcaatta aagtatgtaa aggatttccg cctgggtgag tttggagaag
     6301 gcggaaccgg cgttaccatc gtgactttta agtaatgaca taaggaggat tttcaccaat
     6361 ggctgaaaaa cagaagattc tgatcgtaga tgatgatgca aatattgcag aacttatttc
     6421 cctgtattta atgaaagaat gttatgaaac aatgatcgta ggagacggcg aggaagccct
     6481 taaggtattc cccgaattta aacccaacct gatccttctt gacctgatgc ttccgggcat
     6541 agacggctat caggtctgcc gggagctgcg cacctcatct caggttccca tcatcatgct
     6601 gtcagccaag ggtgaaatct ttgacaaggt tctggggctg gagctgggag cagatgacta
     6661 tatgatcaaa ccctttgatt ccaaagagct ggtcgcccgt gtaaaagcag ttctccgccg
     6721 ttatcaggca gcccctgcct ccatttctac agaaccaaag cagcagggag aatatgtgga
     6781 gtacccggat ctgatcgtaa acctgacaaa ctactctgta ctctacaaag gccattcggt
     6841 ggaaatgcct cccaaagagc tggagcttct ctactttctg gcctcctcgc cgaatcaggt
     6901 ctttacaaga gagcagctcc tggatcacat ctggggctac gaatacatcg gagatacccg
     6961 aaccgtagac gtacatatca agcgtctgcg ggaaaaaatc aaggatcacg ccaactggtc
     7021 cattaccacg gtatggggca tcggctacaa attcgaggta aagaggtaat accatgaccc
     7081 gttcactcta ctccaaattt atcctgggct atctgatctt cggcctcctg ggcttcatca
     7141 ccattgccac cttttcctcc cgcatgacca gagactatct gatgcgggag cggtcggagg
     7201 ctctttatga tgaggcaaat gatattgcag cctcctgcag tcagatgtac gacggaaagc
     7261 ggccggatct ggctgccttt tcctcccagt taaaaagtct gggcgcctat ctgcgcgcag
     7321 aaatatgggt ggcggacaac caaggggcca tttttatgga cagccgggac ggcagccgga
     7381 cacagactgt aatcccggat ttcgacccca ctgcctcagg aagccgctcc tacaccattg
     7441 ggaattatta tggccttttt aatgaggacg ttctcaccgt atctgctccg gtgatcggaa
     7501 attacactac ctacggctat gtgatccttc atctgccggt tagccagatc gcccattccc
     7561 agagtgaaat cctggacatt ctctacatca cctctgcggc cattttcgga ctgtccctca
     7621 ttatccttct ggtctttacc cagaccgtat atcttcccct gcgaaaaatc acagtaggag
     7681 ccaaggaata tgcagccggc catctggatt accggataca ggtaaagacc catgatgaaa
     7741 tgggctacct gtccgatacc ttaaactata tgtcagatga actggataaa atggaggaat
     7801 atcagaggaa ttttatcgca aatgtttccc acgatttccg ttccccctta acctccatca
     7861 aaggctatct ggaagcaatc ctggacggaa ccattccgcc ggagctgtat gaaaaatata
     7921 tttccagagt tatttccgaa acagaacggc ttcacaagct gacggaaagt atgctgacct
     7981 taaattcact ggatgccaga ggatacctga cgcggacgaa ctttgatatt aaccgtgtca
     8041 tcaaggatac cgcagcctcc tttgaaggta cctgcggcaa aaaaaatatc agctttgatc
     8101 tgactttttc cgataatatc cagatggtct atgctgatat ggaaaaaata cagcaggtaa
     8161 tgtacaacct gatcgacaat gccatcaagt tcagccacaa tgattctgtg atctatatcc
     8221 aggcggcagg acagtatgaa aaggttttcg tttccgtaaa ggataccggt atcggcattc
     8281 caaaggacag cctgaaaaaa atatgggaac gcttttacaa aaccgatctt tcccgcggaa
     8341 aagacaaaaa aggaaccgga cttggccttt ccatcgtaaa ggaaatcctc cagtcccatg
     8401 gagaaaatat tgacgtgatc agcaccgaag gcgtgggaac agaatttatc ttctcacttc
     8461 cccgatcaac ggttctgtag actttccaga ccacccattt tatggtatac tgatggtatc
     8521 attatttgta aataccagaa ttttaaatgc ataaactctg acaggaaagg atggatgact
     8581 atgacccgaa ccaaacaggc agccgctttt tttatgaccg cagcccttct ctcttctgcc
     8641 tttgctcccg cagccctggc tgacaacaaa acaaaggtag gaaagatctg cctgaccgtc
     8701 gacagcgata tccgagccgg cagcagtggc ggtgaagtag atgtaacacc cacaggagac
     8761 aacacggatc tgtactatgt agatgccgtt gaagttgtca atgatgacgg ggacgactgg
     8821 tccaaatcca atcctcctga ggtagatatt acccttggtc tggaggacga ggaagaatac
     8881 gccttttccg gaacctcctc cagcagcttc aagctcactc tggacagctc catcaaatcc
     8941 cgctttgaca agatcaaatt cgtagacgcc cgaaaaacag acggcggagc tactgtcatc
     9001 ctgactgtaa agctggtttt tgacgaagac gccgatatga gcgacgcgcc tgcccccgga
     9061 aacctgtcct gggattctga caatccaaag gtcgctgtat gggatgacgt tgaatctgcc
     9121 aaatatttcc aggtccagtt atatcggaat ggctccctcg taacgcctcc tgacggcgga
     9181 aactctactt tgtctgtata taataccttc tatgacttta cggactggat gacggaaaat
     9241 ggaacctaca catttaaagt gcgttctgta aaagccagca acaatgccaa gagcaactgg
     9301 aaaacttccc ctgaacaaac agccggaaat tctgtctccg aaggctggaa acaggcagcc
     9361 gaccatgtgc gctggtggtg gcagaacgca gacggaacct atcctgcatc ccagtggaag
     9421 gatgtaaacg gccagtggta ttattttgac gcagacggct acatggcaac cggctggata
     9481 caggtaaacg gcctgtacta ctatctggat ccctcctccg gcgcaatgta tgcaaactgc
     9541 tatacaccgg ataactgctg ggtggatgca aatggagttt ggattccata gtctttttat
     9601 aattgaaaat agccgaaaaa acaatcgtaa aaatttgcga ttgttttttt gtgcgttttt
     9661 gcgtataata caattaataa aacccagaag gatgtgattt atatgcgtga aacacgaaca
     9721 ctggaattta aggaaac
//
LOCUS       DBMDUH010000100         9692 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_3351_length_9692_cov_3.652904, whole genome shotgun sequence.
ACCESSION   DBMDUH010000100 DBMDUH010000000
VERSION     DBMDUH010000100.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 9692)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9692)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9692
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3351_length_9692_cov_3.652904"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="ACZPCG_09085"
     CDS             complement(<1..568)
                     /locus_tag="ACZPCG_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009268627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR1285244.1"
                     /translation="MIPNTNYSELKDSYLFYHIAQKTKAYLNSHPDSYLYRMGIGDVS
                     LPLCDAVVEALHRAVDDQSQKSTFHGYMPECGDPGLRETIADYYKKRKVSLLPEEVFV
                     SSGASDELGDILDLFGRDKTVMIMEPAYPAYVDANIIAGNRIIHIPSGKENGFLPLPD
                     ETTAADVIYICSPNNPKIIFFNFVPPIYI"
     gene            complement(565..3756)
                     /gene="carB"
                     /locus_tag="ACZPCG_09090"
     CDS             complement(565..3756)
                     /gene="carB"
                     /locus_tag="ACZPCG_09090"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006864023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HIR1285245.1"
                     /translation="MAKRTDLKKILVIGSGPIVIGQAAEFDYAGTQACLALKEEGYEV
                     VLCNSNPATIMTDTSIADKVYMEPLTLEYVAKIVRHERPDAIVPGIGGQTGLNLAMQL
                     EKKGILKECRTELLGTSSRSIETAEDRELFKELCERLGEPVLPSLVANTVEEAVKAAG
                     QIGYPVVIRPAFTLGGTGGGFAEDETEFLAMIKNALALSPVHQVLIEKSVKGFKEIEY
                     EVIRDANDTAITVCNMENLDPVGIHTGDSIVACPSQTLTNKEYQMLRDSALKIIRALK
                     IEGGCNVQFALDPNSFQYYLIEVNPRVSRSSALASKASGYPIARVSAKISVGMTLDEI
                     MLVNTPASFEPALDYVVTKMPRFPFDKFTDANQKLGTQMKATGEVMSVGRTFEESLLK
                     AVRSLETGVFHLYMEKFEKEDRNALMDYIRIGTDDRIFAIARLLRLGEPISAIYKQTG
                     IDPWFLSKMKHITDMEQELKTGIGDRELLYQAKKNGFSDRAVGWLWNMTESQVYEMRK
                     KQGIFPVYKMIDTCASEFESYVPYFYSTYEEENESVIENRKKIVVLGSGPIRIGQGVE
                     FDYSTVHAVKTIKEAGYEAIIINNNPETVSTDYTTSDKLYFEPLCVEDVMNVIQMEQP
                     DGVIASLGGQTAINLAKPLEERGVTLIGTGCAAIEKAENRDAFEKLLLELKIPQPKGQ
                     AVTDLEEGVQAAEAIGYPVLVRPSFVLGGRAMQIVAKEEQMRHYLKTAVEIDKDKPVL
                     VDKYICGKEVEVDAICDGREIFVPGIMELVERTGVHSGDSISVYPAFSISEKVKETIL
                     EYTRKLGLGIGIVGLYNIQFIVDKKEQVYIIEVNPRSSRTVPFLSKATGYPLADMATK
                     VILGENLEKQGITCMYPQEKTRWYVKVPAFSFSKLQGMDAYLSPEMKSTGEAIGYDKK
                     LHRAMYKAMIASGIRVQNYGTVIVTLADEDKEEAVPLIRRFYDMGFNIEATSSTAETL
                     KHHGIRTKVLRKPSEGSREILDAISAGYVSYVINTRAILSGIHYEDGAAIRSAASQNH
                     VTMFTSLDTVRVLLDVLEEITIGVSAITEEENI"
     gene            complement(3740..4840)
                     /gene="carA"
                     /locus_tag="ACZPCG_09095"
     CDS             complement(3740..4840)
                     /gene="carA"
                     /locus_tag="ACZPCG_09095"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HIR1285246.1"
                     /translation="MKKFDRKLILEDGSEYYGYGFGSDGERITEIVFNTSPVGYQEII
                     SDPSYTYQTVVMAYPLIGNYGITEEDFETETPTIGGLAVRDYNDQPSNFRSVCTLSEM
                     MEKFGIPGISGIDTRKLVRSIRNLGSRKALLTDADTSLQEGLLKLAAYKIPTDAVERV
                     SRKTVQKYEVSGGTYHVAAIDCGMKQNIVRCFNRRGCSVTVFPWNTSAEEIEGIKPDG
                     IFLSNGPGDPADARPVIELVKKLRGKYPMFGICLGHQIISLAYGARTYKLRFGHRGGN
                     HPVKNLETGKIEITSQNHSYAVDAESLSGTGLEATHFNLLDQTIEGAACREDRVFSVQ
                     YHPESAPGPQDSGYLFDQFIDRMEETKHGKTN"
     gene            complement(4844..6277)
                     /gene="purF"
                     /locus_tag="ACZPCG_09100"
     CDS             complement(4844..6277)
                     /gene="purF"
                     /locus_tag="ACZPCG_09100"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HIR1285247.1"
                     /translation="MGLHEECGVFGVMGKKAEPMAELVYYGLYALQHRGQESCGIVVN
                     DDGVFSSYKDLGLISEVFSKEVLSHFPEGTMAVGHVRYGTTGGNTRKNCQPIEVNHQK
                     GKMALAHNGNLSNALELRDKLELSGAIFHTTSDTETIAYIITRERMKTPSIEDAVSNA
                     MEYLEGAYSLVLMSSAKLIAARDPYGFRPLCYGTMSDGTYVVASESCALAAVGADFVR
                     DILPGEILVFHQDGVESRREHCGTQKKKTCIFEYIYFSRPDSVIDGVSVHESRRKAGE
                     LLAERYPAEADIVIGVPDSGLDAALGFAEKAGIPYGIGLIKNKYIGRTFISPGQRERL
                     DQVRIKLSPVKSVIEGKRVILIDDSIVRGTTSKRIVKLLREAGAREIHMRISAPPFLH
                     PCYYGTDIDSEKNLIACHHSTEEIAEIIGVDSLGYLEVELLERLIGCRDYCAACFQGE
                     YPTKIPTDLRKDRFERKLSEQVQGKRS"
     gene            complement(6385..7998)
                     /locus_tag="ACZPCG_09105"
     CDS             complement(6385..7998)
                     /locus_tag="ACZPCG_09105"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HIR1285248.1"
                     /translation="MTKYIFVTGGVVSGLGKGITAASLGRLLKARGLKVAAQKLDPYI
                     NVDPGTMSPYQHGEVYVTEDGAETDLDLGHYERFIDENLNQYSNLTTGKVYWNVLNKE
                     RRGEYLGSTVQVIPHITNEIKEFVYRVGKKTDADVVITEIGGTIGDMESQPFLEAVRQ
                     ISLEVGSENSLFIHVTLVPYLSGSEEHKSKPTQHSVKELRGMGINPGLIVLRSDRPLE
                     ETIFRKISLFCNVKEDCVIENRTLGNLYEAPLMLEKSGFSDVVCRELHLEAPAPDLAD
                     WEEMVERIRNRSEEVRIGLVGKYVKLHDAYLSVAEAMSHAGYEQNTFINIRWIDSETI
                     TKENVEDMLADLDGVIVPGGFGNRGIEGMILAADYAREHQIPYFGICLGMQIGVITFA
                     RNVMGIKDAHSGEFDEQCAHKVIDFMPGQSEEIEKGGTLRLGSYPCSIKAGTKMEACY
                     GASLIHERHRHRYEFNNEYREEMERAGLVISGTSPDGRLVEAIELPDHPFFVGVQFHP
                     EFKSRPNQAHPLFRGFISAALRHAKGKEE"
     gene            complement(8041..9621)
                     /gene="asnB"
                     /locus_tag="ACZPCG_09110"
     CDS             complement(8041..9621)
                     /gene="asnB"
                     /locus_tag="ACZPCG_09110"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004065131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HIR1285249.1"
                     /translation="MCSIMGYCSSSAVLSNFMEGFQKTISRGPDDTKVVDTGNGILGF
                     HRLAIMGLAPSGMQPFKLGDSYVVCNGEIYGFEKIKKALSEKYTFESDSDCEILLPMY
                     QEYGTDMFAMLDAEFACIIYDGETGEFIAARDPIGIRPLYYGYDKNHAIVFASEPKNL
                     IGLTDRIMPFPPGHYYKGGQFICYCDIAETDTICQDDLETVCRKIHDKLVAGVEKRLV
                     ADAKVGFLLSGGLDSSLVCAIAAKKSGKPIKTFAIGMREDAIDLKYARQVADYIGSNH
                     TEVYMTPEEVLTSLETVIQLLGTYDITTIRASIGMYLLCKAIHNQTDIRVLLTGEISD
                     ELFGYKYTDFAPDGKAFQQESRKRIRELHMYDVLRADRCISVNSLEARVPFGDLDFVK
                     YVMAVNPELKRNTYGKGKYLLRRAFEQGGYLPDNILWREKAAFSDAVGHSMVDYLREY
                     AESKYTDEEFESKCRRYTHARPFTKESLLYREVFETYYPGQAEMIKDFWMPNKAWEGC
                     NVNDPSARVLSNYGDSGK"
BASE COUNT         2318 a         2684 c         1948 g         2742 t
ORIGIN      
        1 ttatataaat tggaggaaca aaattgaaaa aaataatttt gggattattg ggcgaacaga
       61 tataaatgac atctgccgcc gttgtctcat ccggcaaggg taaaaaacca ttctcctttc
      121 cggaaggaat atgaatgatc cggtttcctg cgattatatt ggcgtctaca taggcaggat
      181 aagcaggttc catgatcata accgttttgt ctcttccaaa cagatccaga atatccccca
      241 gttcatcgct tgcgccgctg gaaacaaaca cttcctctgg caaaagggat acctttcttt
      301 tcttataata atctgcaatg gtttcccgca gtcccggatc cccgcactcc ggcatatagc
      361 cgtgaaaagt acttttctgg ctctgatcat ctaccgccct gtgaagagct tcaacgaccg
      421 catcgcaaag gggaagagat acatctccga ttcccatccg atacagatag ctgtctggat
      481 gactgttcag gtaggccttt gttttctgtg caatgtggta aaacagatag gagtctttga
      541 gctcactgta atttgtattt ggtatcatat attttcctcc tccgttatgg cagaaacgcc
      601 aatggtaatt tcttccagaa catccagcaa tacccttacg gtatcaaggg aagtaaacat
      661 agtaacatga ttctgcgatg cagcgctccg gattgctgcg ccgtcttcat agtgaatgcc
      721 ggagagaatg gcacgggtat taatcacata actgacatat ccggcgctta tggcatccag
      781 gatttcccgg ctgccctccg acggctttct cagaactttt gtacggattc catgatgttt
      841 cagagtctcg gctgttgatg aagttgcttc tatattaaat cccatatcat agaaccgacg
      901 aatcagagga acggcttctt ccttgtcctc gtctgcaagg gtcacaatca ctgttccata
      961 attctgtacc cggataccag aagcgatcat ggccttgtac attgcccggt gaagcttttt
     1021 atcatatcca atagcctctc ccgtagactt catttccggt gaaagataag catccatacc
     1081 ctgtaatttt gagaaggaaa atgcaggaac cttcacatac catcgtgtct tttcctgcgg
     1141 atacatacag gtaatgccct gcttctccag gttttctcca aggataacct ttgttgccat
     1201 atcggccagg ggatagcctg tggcttttga cagaaaggga acggtccggg aagaacgggg
     1261 attgacctca ataatataca cctgttcttt cttatccaca ataaactgga tattataaag
     1321 ccccacaatc ccaattccaa ggcccagctt tctggtatac tctaaaatcg tctccttaac
     1381 cttttccgag atgctgaaag ccggatacac gctgatggaa tctccggaat ggacgccagt
     1441 ccgttctacc agttccataa tcccaggtac aaatatctcc ctgccgtcac agatggcgtc
     1501 aacctccact tccttgccgc agatgtattt gtccaccagc actggcttgt ccttatcaat
     1561 ctccactgcc gttttcagat aatggcgcat ctgctcctct ttggccacga tctgcatggc
     1621 tctgccgccc agtacaaagc tggggcgcac taaaacggga tagccgattg cctctgccgc
     1681 ctgcacgccc tcttccagat cggttaccgc ctgccctttg ggctggggga ttttcagttc
     1741 caataacagt ttttcaaagg catctctgtt ttccgctttc tcaatggcgg cgcagcctgt
     1801 gccaatcaat gttactcccc gctcttccag cggctttgcc agattgatgg ccgtctgccc
     1861 gcccagggaa gcaatgactc cgtccggctg ttccatctgg atcacattca tgacgtcttc
     1921 cacgcacagg ggctcaaaat acagcttatc ggatgtagtg taatcggtgg atacggtttc
     1981 cggattgttg ttgataataa ttgcctcgta tccggcctcc ttgatcgtct ttaccgcatg
     2041 aaccgttgaa tagtcgaact ccactccctg cccaattcgg atcgggccgg agcccagcac
     2101 cacaatcttt ttcctgtttt caattaccga ttcattttcc tcttcatagg tagagtaaaa
     2161 atagggcaca tagctttcaa attccgatgc acaagtatcg atcattttat aaacagggaa
     2221 gatcccctgc tttttcctca tttcataaac ctggctttca gtcatattcc acagccatcc
     2281 gactgccctg tcagaaaaac cgtttttctt cgcctggtat agtaattccc tgtctccgat
     2341 gcctgtcttc agctcctgtt ccatatcggt aatatgtttc atcttagaaa ggaaccatgg
     2401 atcaatccct gtctgtttat agatggcaga aatcggctca cccagccgca gcaggcgggc
     2461 aatggcaaag atcctatcat cggtaccgat ccggatataa tccatcaggg cattcctgtc
     2521 ttccttttca aacttttcca tatacagatg gaagaccccc gtttccagag aacgaacagc
     2581 ttttaaaaga ctctcctcaa aggttcttcc cacgctcatg acctctccgg tcgctttcat
     2641 ctgtgtcccc agtttctgat tggcatctgt aaatttgtca aaagggaaac ggggcatttt
     2701 ggtaaccaca taatccagcg ccggttcaaa agaagcgggt gtattgacca gcataatctc
     2761 atccagtgtc atgcctacgc tgatctttgc cgacactctt gcaatgggat aaccgcttgc
     2821 cttggatgcc agggcagacg aacgggatac ccggggattt acttcgatca ggtaatactg
     2881 gaacgaattg ggatccagcg caaactgcac gttgcagcca ccctcaattt tcaatgcccg
     2941 gatgattttc agagcgctgt cccgcagcat ctggtattcc ttattagtta acgtctggga
     3001 aggacaagcc acaatggaat ctcccgtatg gattcctacc ggatccagat tttccatatt
     3061 gcataccgta atggctgtat cattggcgtc ccggatcacc tcgtattcaa tttctttaaa
     3121 tccttttaca cttttttcaa ttaacacctg atgaacagga gacagagcaa gggcattctt
     3181 tatcatcgcc agaaactcgg tttcatcttc tgcaaaaccg cctccggttc ccccaagagt
     3241 gaaagccgga cggatgacca ccggatatcc gatctggccg gccgctttta ccgcctcctc
     3301 cactgtgttg gctaccaggg aaggaagcac cggctctccc agtctttcac acagttcctt
     3361 aaaaagctct ctgtcttccg ccgtctcaat gctcctgctg ctggttccca gaagctctgt
     3421 ccggcactcc tttaaaatgc cctttttttc cagctgcata gccagattca gcccggtctg
     3481 gccgccaatt ccaggaacaa tggcatccgg ccgctcatgg cggacaatct tggccacata
     3541 ttccagagtc agcggctcca tgtatacctt atccgcaata gaagtatcgg tcataatcgt
     3601 tgccggatta gaattgcaca gcactacctc atagccctct tccttcagtg caaggcatgc
     3661 ctgggtacct gcgtagtcaa attccgctgc ctggccaatc acgatcggac cggagccaat
     3721 gacaagaatt tttttcagat cagttcgttt tgccatgctt tgtttcctcc atcctgtcta
     3781 taaactggtc aaacagataa ccgctgtcct gggggccggg agcgctttcc gggtggtact
     3841 gcacgctgaa tacccgatcc tccctgcagg cagcaccctc aatggtctga tccagcaaat
     3901 tgaaatgcgt tgcctccaac ccggttccag aaaggctttc tgcatccaca gcataggaat
     3961 gattctggga ggtaatctca atctttcctg tttccagatt ttttaccgga tggttgccgc
     4021 ctctatggcc aaatctcagc ttataggttc tggctccata agcgagagaa atgatctgat
     4081 gccccagaca gattccaaac atcggatatt ttccccgcag cttttttacc agttctatca
     4141 ctggccgggc gtctgccgga tctcccggtc cattggacag aaaaattcca tctggcttta
     4201 tgccttctat ttcttccgca gaagtattcc agggaaacac cgtcacactg cagcctctgc
     4261 ggttgaaaca ccgcacaatg ttctgtttca ttccgcagtc gatagctgcc acatgataag
     4321 ttcccccgga aacttcatac ttctgtacgg ttttccggct cacccgctcc actgcgtctg
     4381 tgggtatttt ataagcagcc agttttaaaa gtccctcctg cagggaagtg tctgcatcgg
     4441 taagcagcgc ctttctgctt cccagattcc ggatggagcg gaccagcttt ctcgtatcaa
     4501 ttccggaaat tcctggaatt ccaaacttct ccatcatctc cgataaagta cacacactgc
     4561 ggaaattcga tggctggtca ttatagtccc ggacagccag ccctccaatg gtcggagttt
     4621 ccgtttcaaa atcctcttct gtaatcccgt aattaccgat caggggatag gccattacca
     4681 ccgtctggta ggtatacgat ggatctgaaa taatttcctg gtatcctacg ggagaagtat
     4741 tgaatacaat ttccgtaatc cgctctccgt cagagccaaa tccatatccg taatattcgc
     4801 ttccgtcttc caaaatcagt tttctgtcaa attttttcat ggtctagctc ctctttcctt
     4861 gaacctgttc agacagtttt ctctcaaaac ggtctttcct gagatccgtc ggtattttcg
     4921 taggatattc tccctgaaaa caggctgcgc aatagtccct gcatccgatg agcctttcca
     4981 gcagttccac ctccagatat cccagagaat ccacaccgat tatctctgca atctcctccg
     5041 tgctgtgatg gcaggcgatc agattctttt cggaatcaat gtccgttcca tagtaacatg
     5101 gatgcagaaa tggcggtgcg gaaattctca tatgaatttc tcttgctcct gcctcccgca
     5161 aaagcttcac aatccgtttg ctggtggtac cccggacaat ggaatcgtca atcaatatca
     5221 cccgctttcc ttcaatgacg ctctttacag gtgaaagttt aatccggacc tgatccagac
     5281 gttccctctg tcccggagaa ataaaggttc ttccgatata tttgtttttg atcagtccga
     5341 tcccataggg aatgccggct ttctccgcaa atcccagagc cgcatccagt ccgctgtcag
     5401 gaactccgat tacgatgtct gcttctgccg gatagcgctc tgccagcagt tcccccgctt
     5461 ttcttctgga ttcatgaacc gacacgccgt caataaccga atctggtctg gaaaaataga
     5521 tatattcaaa aatgcaggtc tttttcttct gcgttccgca gtgctctctt ctggattcca
     5581 ctccatcttg atgaaagacc aaaatttctc ccggcaggat atctctgaca aagtctgccc
     5641 ctaccgccgc cagcgcgcaa ctttctgagg ccaccacata ggttccgtcc gacatggttc
     5701 catagcacag cggccgaaaa ccatagggat ccctggcagc aatcagtttt gcggaagaca
     5761 tcagcaccag agaataggct ccttccagat attccattgc attgctgacc gcatcttcga
     5821 tgctgggcgt tttcattctt tccctggtga taatgtatgc aatggtttcc gtatcgctgg
     5881 tcgtatggaa aatagcccca gacaattcca gcttgtcccg aagctccagc gcattgctta
     5941 agttgccgtt atgggccagc gccatctttc ccttctggtg gttcacttca atgggctgac
     6001 agttcttcct ggtattgcct ccggtggttc cgtatcttac atgacctact gccatggttc
     6061 cctccggaaa atgagataat acctctttgg aaaacacttc gcttatcagc cccagatctt
     6121 tataggaaga aaatacgccg tcgtcattca ccacgatgcc gcagctttcc tgtccccggt
     6181 gctgcagcgc ataaagccca taataaacta actccgccat aggttctgcc tttttcccca
     6241 ttacgccaaa tactccgcat tcttcatgca gtcccatcct gttgttcctc ctgtcccttc
     6301 atccttactc tacggtcaca gattttgcca gatttctcgg cttgtcaata tcacagcccc
     6361 ggttcaatga tcccacacat gattctattc ctcttttcct ttcgcatgtc tgagtgccgc
     6421 tgaaataaat cctctaaaca gcggatgagc ctggttgggg cggctcttaa attccggatg
     6481 gaactgtacc cctacaaaaa atggatgatc cggcagttct atggcttcca ccagcctgcc
     6541 atccggagac gtaccgctga tcaccagtcc cgcgcgctcc atctcttccc gatactcatt
     6601 attaaactcg tagcgatggc gatgacgctc atgaatcagg gaagcgccgt aacaggcttc
     6661 catctttgtt cctgccttaa tgctgcaggg atagctgccc agccgcaggg tgcctccctt
     6721 ctcaatctcc tcactctgtc caggcataaa gtcaatgact ttatgagcgc actgttcatc
     6781 aaattctcct gagtgagcgt ctttaatccc cattacattt ctggcgaatg taatcactcc
     6841 aatctgcatg cccagacaga tgccaaaata tggaatttga tgttcacggg cataatctgc
     6901 agccagaatc atcccttcaa ttccacggtt tccaaagccg cctggaacaa tcaccccatc
     6961 cagatctgcc agcatatctt ccacattttc ttttgtaatg gtttccgaat caatccagcg
     7021 aatgttgata aaggtattct gttcatatcc tgcatggctc atggcctctg ccacagacag
     7081 gtaggcgtca tggagcttta catactttcc aaccagccca atacggactt cctccgacct
     7141 gttccggatc cgttctacca tctcttccca gtcagccaga tccggcgccg gcgcctccag
     7201 atgcagctct ctgcagacta catcggaaaa accggacttt tccagcatca gcggcgcttc
     7261 ataaagattc cccaatgttc tgttttcaat gacacaatcc tcttttacgt tgcagaacag
     7321 ggaaattttc ctgaaaatgg tctcttccag cggtctgtct gaacgaagca caatcagacc
     7381 tggattgatc cccattcccc gaagctcctt gactgaatgc tgggtgggct tggacttatg
     7441 ctcctccgat ccgctcagat aaggaactaa ggtcacatga atgaacaggc tgttttcgct
     7501 tcccacttcc agggaaatct gccgcaccgc ttctaagaag ggctgagatt ccatgtctcc
     7561 gatggttcct ccaatttccg taatcaccac atcggcgtca gtctttttgc ccacacggta
     7621 cacaaattcc ttaatttcat tggtaatgtg agggatcacc tgaaccgtag aacccagata
     7681 ctcgcctcgt cgttccttat tcagaacgtt ccagtacacc ttcccggtgg tcaggttcga
     7741 atactgattg agattttcat cgataaaccg ttcgtaatgt cccagatcca gatctgtttc
     7801 tgccccgtcc tccgtcacat aaacttcccc gtgctgatag gggctcatgg ttcctggatc
     7861 tacattaata tagggatcta atttctgagc cgctaccttt aagcctctgg cctttaaaag
     7921 ccgcccaaga gaagcggcgg taatgccttt ccccagaccg gacaccacgc ctccggttac
     7981 aaatatatat tttgtcatac ttttcacttt cctcagaaca tggcaatgta atcttcgttg
     8041 ttattttcca ctgtctccat aattagagag aactcttgca gaaggatcgt ttacattaca
     8101 gccttcccat gccttgttcg gcatccaaaa atcttttatc atttccgcct gtcccggata
     8161 atatgtttca aaaacctctc tgtacagtaa agattctttt gtaaatggac gggcatgagt
     8221 atagcgcctg catttgctct caaactcttc atccgtatat ttactttccg catattccct
     8281 cagatagtcc accatggaat ggcctactgc gtctgaaaat gccgctttct ccctccacag
     8341 aatattatcc ggcaggtaac cgccctgttc aaaagcacgg cgaagcaaat attttccctt
     8401 tccgtatgta ttcctcttta attccgggtt gacggccatc acatacttta caaaatccaa
     8461 atctccaaag ggaactcttg cttccaggga gtttacagaa atgcagcgat ctgcccgcag
     8521 tacatcgtac atatgcagtt ctctaatccg tttccgggat tcctgctgaa aagctttccc
     8581 atctggtgcg aaatctgtat atttatagcc aaacagctcg tcagaaattt ctccggttaa
     8641 caggacccgg atatccgtct ggttgtggat cgccttacac agcagataca tacctataga
     8701 tgcgcggatc gtagtaatgt cataggttcc caacagctga atcacggtct ccagagaggt
     8761 cagcacttct tctggcgtca tgtaaacctc tgtatggtta cttcctatat aatctgccac
     8821 ctgtcttgca tattttaagt caatggcgtc ctctctcatt ccaatagcaa aagttttgat
     8881 gggtttcccg cttttctttg ccgctatggc gcacactaaa gaagaatcca ggccgccgga
     8941 aagcagaaat cctacctttg cgtctgcaac caggcgtttc tccacgcctg ccaccagctt
     9001 gtcatggatc tttctgcaga cggtttccaa atcatcctgg cagatcgtgt ctgtttctgc
     9061 aatatcacaa tagcagataa actgtcctcc tttataataa tgtcctggcg gaaacggcat
     9121 aatcctgtct gtaagtccta tcagattttt cggttcactg gcaaacacaa tggcgtgatt
     9181 tttatcatat ccataataca atggacggat tccaatcgga tcccttgctg caataaattc
     9241 ccctgtttcc ccatcgtaaa tgatacaggc aaactcagca tccagcatag caaacatatc
     9301 cgtgccatat tcctgataca tcggcaggag gatttcgcaa tccgaatcgc tttcaaacgt
     9361 atatttttct gacagtgctt tttttatttt ctcaaatccg taaatttctc cattgcagac
     9421 cacatagctg tctcccagct tgaaaggctg cattcctgac ggggccagtc ccataatagc
     9481 taatctatgg aatccaagaa tcccattgcc tgtatcaacc actttggtgt cgtctggtcc
     9541 ccttgatatt gtcttctgaa acccttccat gaaattgctg agaaccgcac tgctgctgca
     9601 atagcccata atggaacaca taggaaagtc cctccttcca atattccttc taagtttcct
     9661 ctggaattta ttttacgctg aacataatat cc
//
LOCUS       DBMDUH010000101         9670 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_3357_length_9670_cov_5.753997, whole genome shotgun sequence.
ACCESSION   DBMDUH010000101 DBMDUH010000000
VERSION     DBMDUH010000101.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 9670)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9670)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9670
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3357_length_9670_cov_5.753997"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /locus_tag="ACZPCG_09115"
     CDS             <1..921
                     /locus_tag="ACZPCG_09115"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002578005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HIR1285250.1"
                     /translation="GNDVDREKTILAVNEAVKAGRNELDLEAEGCYRTVSLWESDEHL
                     KNLCDAMNSRREKKLRYVFGEAEEFLTGETMASWITGVLEGKITVDQEKVAAFVKDLA
                     SRYDTAGTARTVTKAAGGQTEITGPYGWKIDQAAETAALMELVSSGSCWSEGQAQADR
                     EPVYSQKAASREGMDWGTTYVEVDLGGQHVYMFKEAQIVWDAPCVTGNVSKDYTTPSG
                     IYSLTYKEKDRILRGKKKPDGTYEYESHVDYWMPFNGGIGLHDASWRSKFGGTLYQTG
                     GSHGCINLPPQKAPALYDLVYKGIPVLCTP"
     gene            complement(988..2211)
                     /locus_tag="ACZPCG_09120"
     CDS             complement(988..2211)
                     /locus_tag="ACZPCG_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AfsR/SARP family transcriptional regulator"
                     /protein_id="HIR1285251.1"
                     /translation="MPSAFDLNPTIYIHTLGGFSIEAGGKVINDSSNQSKKPWALLEY
                     LAIFQKKDISPAELIQVIWPDDPGVNPAGALKTLMFRSRKLLEPLDIPPQKLLVQQRG
                     TYAWTKEYTTVLDIDEFETICTRVLNESLPEEEALSLCFAGVELYRGDFLPKAEYESW
                     VIPISTYYHTLYQKLIHYTIHLLTAKKAFSQITALCQTAIAIEPFDEEFRYQLVYSLY
                     RDGHTHQALEEYNNTLDLFYSEFSISPSDHFKDLYKIIRDDEQSVNLNLDHIQHALSE
                     ESSGGAFYCEYPVFRDLFQLERRAIDRTGDSIYLCMLTVTGLNGKSLKTLILNKAMEH
                     LEASIRGSLRCSDVFTRYSISQYLILLPTITSEMAEMVLKRIITNFRKVYNRKDMTVE
                     FRLQPLKPLNRDEGF"
     gene            2389..3243
                     /locus_tag="ACZPCG_09125"
     CDS             2389..3243
                     /locus_tag="ACZPCG_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285252.1"
                     /translation="MTKKGLLPNAYRFIHIAADGFEDRILKGRLYHDSREGGMPFIGL
                     SEMILILEELFDEIHYPIKSVDYRNFLGKSRELKAETGEMEASQLLPERKGKKADFTL
                     HVKYRYNATWQGSITNLGTGERKSFLSLMELMDILAVSLGGQIRPGGTGLGKRMCEVT
                     VRNYEGCQMGGDVSHPAVADRRTFINEFDLKEEIAAMLDPLPEGSRPDGIIIPRSFHV
                     AVGNYGPATFVVRILFRRNATWQGTVSWREKRSQVNFRSFMELLLLMQEAVGRTGEWK
                     QQERNISV"
     gene            3240..4124
                     /gene="srtB"
                     /locus_tag="ACZPCG_09130"
     CDS             3240..4124
                     /gene="srtB"
                     /locus_tag="ACZPCG_09130"
                     /EC_number="3.4.22.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0045184 - establishment of protein
                     localization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HIR1285253.1"
                     /translation="MNENERKSGGKRRVLMFILLAVFLISGALTVHQYLEDKRIQEEY
                     ERLAEEAARQTTEAPTEPETEETEPLEEAYVSPIDFEALTAENPDTIGWIRIPDTNVD
                     YPIVQGTDNDFYLDHDFYGKESAAGSIYLDFESEADLQGRNQILYGHNMKNGSMFKDI
                     NRYKDPEYFKEHQYFSIYTPEREICLKAVSCYYGEAKPIVRMTGFKTQETFDEFVRTM
                     VEPCSYKEEVEYPARSIFTLVTCSYEINDARTFLFAVEVDEEGNQIPMDEEYKEKMET
                     VLEKKLKASENISDENGK"
     gene            4326..5201
                     /locus_tag="ACZPCG_09135"
     CDS             4326..5201
                     /locus_tag="ACZPCG_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIR1285254.1"
                     /translation="MKKNVKRMMALAAAGILAAGALTGCGGGADTAKAEGAAASGEKK
                     ILKVAMECAYAPYNWTQPDDSNGAVPISGSNEYAYGYDVMMAKKICEELGYDLEIVRL
                     DWDSLIPAVTTGQADCVIAGQSITSERLQAVDFTVPYYYASIVTLVKKGSKYENAKSV
                     ADLAGATCTSQQSTIWYNTCLPQIQDANILAATANAPDMLMSLNADKCDLVVTDQPTG
                     KGALIAYPDFKLIEFGGGENDFQVTDEDINIGISLKKGNTELKEAIDSVLSKMTKEDF
                     SKMMDEAISVQPLVN"
     gene            5250..6047
                     /locus_tag="ACZPCG_09140"
     CDS             5250..6047
                     /locus_tag="ACZPCG_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006781758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR1285255.1"
                     /translation="MPTDFFGRVMAVFSRYGVSMLQGAGVSLQIALVGTIAGCVIGFA
                     VGIVQTIPSEKGDNPIKRGILWIVKLILNIYVEFFRGTPMMAQAMFIYYGLMPLLNIN
                     SSILNAAYFILSINTGAYMAETVRGGILSIDKGQTEGAMSIGMTHVQTMLYVILPQAL
                     RNLMPQIGNNLIINIKDSCVLSVIGVAELLYKTKAAAGALYMNFETYTITMVMYFIMT
                     FACSRLLRLWEKKMDGADNFDLATTDTLAYTSGMYRYPDDRDKKEGV"
     gene            6047..6799
                     /locus_tag="ACZPCG_09145"
     CDS             6047..6799
                     /locus_tag="ACZPCG_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005427770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIR1285256.1"
                     /translation="MSEKILEIRHLSKTFGTNLVLKDIDFTVNKGDVTCIIGASGSGK
                     STLLRCLNLLETPTTGEILYHGNNIADPKVNAAKYRSRVGMVFQSFNLFNNMTVLENC
                     VVGQVKVLKKDKETARKNALSYLEKVGMAPYINAKPKQLSGGQKQRVAIARALAMEPE
                     LLLFDEPTSALDPQMVGEVLEVMRKLASEGLTMIIVTHEMAFARDVSSQVVFMAGGVI
                     VEEGEPAQIFGAPKEHQTKEFLGRFMHERAAL"
     gene            complement(6885..7070)
                     /locus_tag="ACZPCG_09150"
     CDS             complement(6885..7070)
                     /locus_tag="ACZPCG_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285257.1"
                     /translation="MEQTRHPAGRHSETQVHKNNYDIDIQACSSMDCTGLIPALPEDE
                     AQREAYEELYPYITKAR"
     gene            7279..7626
                     /locus_tag="ACZPCG_09155"
     CDS             7279..7626
                     /locus_tag="ACZPCG_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007714578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HIR1285258.1"
                     /translation="MEYFFMGGFEIMFLMVFVLIFGMILVMIVRGIGEWGKNNRSPRL
                     DVSATVVSKRTHTMRNQNHTSSSSYYVTFQVESGDRMELRLGGREYGLLAEGDKGKLH
                     FQGTRYLGFDRNW"
     gene            7655..7798
                     /locus_tag="ACZPCG_09160"
     CDS             7655..7798
                     /locus_tag="ACZPCG_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285259.1"
                     /translation="MTHLKGNFILLLLDLWMDCHYNRKEWVLARFMRVKAACSEGEIA
                     QYG"
     gene            7791..>9670
                     /gene="ftsH"
                     /locus_tag="ACZPCG_09165"
     CDS             7791..>9670
                     /gene="ftsH"
                     /locus_tag="ACZPCG_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIR1285260.1"
                     /translation="MDNNKKQNQNMNSNWQTIAMLVVAALLTIMIVSWMNSAVESRQR
                     KELSYNEFVQMVEDGKVESIRVKDAVIEVMPKAEATEYSQQMKYYVVRIEGDYQFLNR
                     VLENNVITNRENGSTNALLIALLNYAVPFLILLFLMNFTMKRMGGGGIMGVGKSNAKM
                     YVQKETGITFKDVAGEDEAKESLTEIVDFLHNPGKYTKIGAKLPKGALLVGPPGTGKT
                     LLAKAVAGEAHVPFYSLSGSDFVEMFVGVGASRVRDLFKNATENAPCIIFIDEIDAIG
                     RSRDSRMGGNDEREQTLNQLLSEMDGFDSTKGLLVLGATNRPEILDPALLRPGRFDRR
                     VVVDKPDLNGRINILKVHSKEVLLDETVDFKEIALATSGAVGADLANMMNEAAINAVK
                     NGRSAVSQKDLFEAVEVVLVGKEKKDRIMSKEERRIVSYHEVGHALVSALQKHSEPVQ
                     KITIVPRTMGALGYVMNVPEEEKFLNTKKELEARLVELMGGRAAEEIVFETVTTGASN
                     DIQQATNLARAMVTQYGMSEKFGLMALESQENQYLTGRAILNCGDATAAEIDKEVMKV
                     LKDAYEAAKSLLNENRDAMDQIADFLIEKETITGKEFMKIFRKVKGIPEPEETPEEEN
                     GLP"
BASE COUNT         2741 a         1941 c         2657 g         2331 t
ORIGIN      
        1 ggaaatgatg tggatcggga aaaaacgatt ttggcagtaa atgaggctgt aaaggcagga
       61 agaaatgaat tggatctgga ggcagaaggc tgctaccgaa cagtcagcct gtgggaaagt
      121 gatgaacatt tgaagaatct ctgtgatgcc atgaacagcc ggagagaaaa aaagctccgt
      181 tatgtattcg gagaggcaga ggagtttttg acaggagaaa ccatggccag ctggatcacg
      241 ggggtattag agggaaagat tacggttgat caggaaaagg tagcagcttt cgtaaaggat
      301 ctggcttccc gatatgacac agctggaaca gccagaaccg taacaaaggc agcaggaggt
      361 cagacagaga tcacaggccc ctatggctgg aagatcgatc aggcagcaga gacggcagcc
      421 cttatggagc tggtttcttc gggaagctgc tggagcgaag gacaggctca ggccgacaga
      481 gagcctgttt acagccagaa ggctgcgtcc agagagggaa tggattgggg aactacttat
      541 gttgaggtcg atctgggagg ccagcacgtt tatatgttca aagaggccca gatcgtatgg
      601 gatgctccct gtgtcacagg aaatgtgtca aaggattata ccactccttc aggaatctac
      661 agcctgacct ataaggaaaa ggatcggata ctcagaggaa agaaaaagcc tgacggaacc
      721 tatgagtatg agagccatgt agattactgg atgcctttta acggcgggat cggccttcat
      781 gacgcatctt ggagaagcaa gtttggcgga acgctttatc agaccggagg cagccacggc
      841 tgcatcaatc ttcctcccca aaaggctccg gcgctgtatg atctggtgta taaggggatc
      901 ccggtactct gtactccata aaatgacccg cccggagatc agaagcactc tccgggcggg
      961 tgataaaaaa ttgtatggaa ggcaggatca gaacccttca tcccgattca agggctttaa
     1021 gggctgtaaa cggaattcaa cggtcatgtc ctttcggttg taaactttcc ggaaattggt
     1081 gatgatccgt ttcagcacca tctcagccat ttctgaagta atcgtaggaa gaagaatcag
     1141 atattggctg atgctgtaac gggtaaatac atcactgcat cttagggaac ctcggatgga
     1201 tgcttccaga tgctccattg ctttgtttaa gatcagggtt ttaagtgatt ttccattcag
     1261 accggttacg gtaagcatac acagatagat ggaatcgccg gtgcggtcaa tggcccgccg
     1321 ctccagctga aacagatcac gaaatacagg gtattcgcag taaaaggcgc cgccggagct
     1381 ttcttcgctg agggcgtgct ggatatgatc cagattcagg ttgacactct gttcgtcatc
     1441 gcggatgatc ttataaagat ccttaaaatg atcagagggg gagatggaaa actcactgta
     1501 aaagagatcc agagtgttgt tgtattcttc cagcgcctga tgggtgtggc cgtcccgata
     1561 cagagaataa accagctgat agcggaattc ctcatcaaag ggttcaatgg caatagcggt
     1621 ctgacacagg gcggtgatct gggaaaaggc tttctttgca gtcagaagat gaatggtata
     1681 gtggatcagc ttttgataca gcgtatggta ataggtgctg atcgggatga cccaggattc
     1741 gtactccgct tttggaagaa aatctcccct gtaaagttca acgccggcaa aacagaggga
     1801 aagggcttcc tcctccggca gagattcatt taacacacgg gtacagatcg tttcaaattc
     1861 atcaatatct aaaacagtag tatattcttt ggtccaggca taggttccgc gctgttggac
     1921 taaaagcttc tgaggcggaa tatccagagg ctctaaaagc tttcttgagc ggaacatcag
     1981 ggttttaagc gctcctgcag gattaacacc cggatcatcc ggccatatta cctggatcag
     2041 ttctgccggg ctgatatcct ttttctggaa gattgccaga tattctaaaa gagcccatgg
     2101 cttttttgac tgattgctgc tgtcgttgat taccttcccc ccggcttcga ttgaaaagcc
     2161 tcccaaggta tgtatataaa ttgtaggatt taagtcaaat gctgatggca tcttttgtaa
     2221 ctccttcata ctaatatatg ttaagtataa ccatataagg ggaaaatgtc tacatcttct
     2281 gccggaaaga aaaagaaaaa tgagaaaata gcccgatttg taaccggatt gtaaccggaa
     2341 actgatatac tagaaaaaag tgacagaaaa aggggatgaa aaatgccaat gaccaaaaaa
     2401 ggtttattgc cgaatgcata ccggtttatc catattgcag cagacggatt tgaagaccgt
     2461 atactgaagg ggagactcta tcatgacagc cgggaaggcg gtatgccttt catcggcctt
     2521 tcggagatga tactgatcct ggaggaatta ttcgatgaga tccactatcc aataaagagc
     2581 gttgactata gaaatttttt aggaaaaagc cgggagctga aggccgagac cggagaaatg
     2641 gaggcttcac agcttttgcc ggagcgaaag ggaaaaaaag ctgattttac gctgcatgta
     2701 aaatatagat acaatgctac ctggcagggg agcattacca atctgggaac aggggaacgg
     2761 aagtcgtttt taagcctgat ggagcttatg gatattctgg cagtttcctt aggcggccag
     2821 atccgtcccg gaggaaccgg tcttggaaag cggatgtgtg aggtaacggt acgaaattat
     2881 gagggctgtc agatgggagg cgatgtatca cacccggcag tagcagaccg cagaaccttt
     2941 atcaacgaat ttgatctgaa ggaggagatc gcggccatgc tggatccgct tccggaggga
     3001 agccggccgg atggcatcat tatcccccga agctttcatg tggcagttgg aaattacggc
     3061 cctgcaacgt ttgtggttcg tattctgttc cggcgcaatg ctacctggca gggaactgtc
     3121 agctggaggg aaaagcgttc gcaggtaaat ttcagaagct ttatggagct tcttttgctg
     3181 atgcaggagg ccgtggggcg tactggtgaa tggaaacagc aggaaaggaa catttcagta
     3241 tgaacgagaa tgaaaggaaa agcggcggaa agcggcgggt gctgatgttt attctgctgg
     3301 ctgtatttct tataagcggc gctttgaccg tacatcagta tttagaggat aagcggatcc
     3361 aggaagaata tgaacgactg gcagaggaag cggccagaca gacaacagaa gctcctacag
     3421 agcctgaaac ggaggaaaca gagccgttag aggaagcgta tgtctctcct attgattttg
     3481 aggcgctgac tgcggaaaat ccggacacca taggatggat caggatccct gatacaaatg
     3541 tggattatcc catcgtgcag ggaacggata atgattttta tctggatcac gatttttatg
     3601 ggaaggaaag tgcggcaggc tccatttatc tggattttga aagcgaagcg gatctgcagg
     3661 gacgcaatca gatcctctac ggccacaata tgaagaatgg aagcatgttt aaggatatca
     3721 accgctacaa ggatccggag tattttaagg agcatcagta tttcagcatc tacacgccgg
     3781 agcgtgagat ctgcttaaaa gctgtgtcct gctactatgg agaagccaag cccattgtac
     3841 gcatgacggg ctttaaaacc caggagacat ttgatgaatt tgtaaggaca atggtggaac
     3901 cctgttccta taaggaagag gtggaatatc cggccagaag tattttcact ctggtaacct
     3961 gcagctatga gatcaatgat gccagaacct ttctttttgc cgtagaggtg gatgaggagg
     4021 gcaatcagat ccccatggat gaggaatata aagaaaagat ggagactgtt ctggaaaaaa
     4081 agcttaaggc atcggaaaat atttccgatg aaaatggaaa ataaggtatt ggaaaaccgg
     4141 aaatgttatg atatactacg catcagaaac atgcagaagt atgtgtgcag tattaaaaat
     4201 ggttcgtgac aaagaagtca tacctgaagg gatgggtgtg acttttttgt ctgttttcca
     4261 ttttttggta tatgcatcag atttgatcat gctttagaag aaagaaaaca atgaggagag
     4321 aaaatatgaa gaagaatgta aagagaatga tggcacttgc agcagcaggc attcttgcag
     4381 caggggcgct gaccggctgc ggcggcggag cagatacggc aaaggcagaa ggagcagcag
     4441 cttctggtga aaagaaaatc cttaaagtgg ctatggagtg tgcctatgct ccttacaact
     4501 ggacacagcc cgatgattcc aacggcgcag ttccgatttc cggaagcaat gagtatgctt
     4561 acggttacga tgttatgatg gcaaagaaaa tctgtgagga gctgggatat gaccttgaga
     4621 ttgtgcgtct ggattgggat tctctgattc cagccgttac gaccggacag gcagactgtg
     4681 tgatcgcagg acagtccatt acttccgagc gtcttcaggc cgttgatttt accgttccct
     4741 attattatgc aagtattgtt accctggtaa aaaaaggaag caaatatgaa aatgccaaga
     4801 gcgtagccga tctggcaggc gctacctgta cctcccagca gagcaccatc tggtacaaca
     4861 cctgcctgcc ccagatccag gatgccaata ttctggcagc cactgccaat gctcctgata
     4921 tgctgatgtc tcttaacgcg gacaagtgcg atctggtagt gacggatcag cctaccggca
     4981 agggcgctct gatcgcatac cctgacttca agctcataga gtttggcggt ggagagaatg
     5041 atttccaggt aacagatgag gatatcaaca tcggaatttc tctaaagaag ggaaatactg
     5101 aactgaagga agccattgac agcgtgcttt caaagatgac aaaagaggat ttctccaaaa
     5161 tgatggatga ggccatttcc gtacagcctc ttgtaaactg atgatctctg ccggcagatc
     5221 ccggcagctt tacaaataag gagaatatta tgccaaccga tttttttggc cgtgtgatgg
     5281 ctgtgttcag ccgttacggc gtatccatgc ttcagggcgc aggagtcagc ttacagattg
     5341 ccttggtggg aaccattgca ggctgtgtca ttggctttgc agtgggcatt gtccagacca
     5401 taccctcaga aaagggagat aatccgatca aacggggaat cctgtggatc gtgaagctta
     5461 ttttaaatat atatgtggag tttttcagag gtactcccat gatggctcag gccatgttta
     5521 tttactatgg actgatgccg ctgttaaata tcaacagctc tattttaaat gccgcttatt
     5581 ttatcctttc cattaacacc ggagcatata tggcggagac tgtccgcgga ggtatccttt
     5641 cgattgataa gggacagacc gagggagcca tgtccatcgg aatgacccat gtgcagacta
     5701 tgctgtatgt gatcctgcct caggcgctga gaaacctgat gccccagatt ggaaataacc
     5761 tgatcatcaa tatcaaggac agctgcgttc tttccgtgat cggtgtagca gagctgcttt
     5821 ataagacaaa ggccgcagca ggtgcgttgt atatgaactt tgaaacgtac accattacca
     5881 tggttatgta ctttatcatg acctttgcat gctcccgtct gctgcgcttg tgggaaaaga
     5941 agatggacgg cgcggacaac tttgatctgg caacaacgga tacgctggca tataccagcg
     6001 gtatgtaccg ttatcccgac gacagagata aaaaggaggg ggtataatga gcgagaagat
     6061 acttgaaatc cgccatttaa gcaagacctt cggcacgaat ctggtcttaa aggatattga
     6121 ttttaccgtc aataaaggcg atgtaacctg tattatcgga gcctccggtt ccggaaaatc
     6181 caccctcttg cggtgtctga accttcttga aacgccaacc acgggagaga ttctgtacca
     6241 tggaaataac attgcagacc caaaggtaaa tgcagccaaa tacagatcaa gagtgggaat
     6301 ggtatttcag agctttaacc tgtttaacaa catgacagtt ctggaaaact gcgtggtagg
     6361 acaggtaaag gtactgaaaa aggataagga aaccgccaga aagaatgctc tttcctatct
     6421 ggaaaaggtt ggaatggctc cctatatcaa tgcaaagcca aagcagctgt ccggcggaca
     6481 gaagcagaga gtcgccattg ccagagcgct ggctatggag ccggaacttc tgctgtttga
     6541 tgagcccacc tcagctctgg atccgcagat ggtaggagag gttttggagg ttatgagaaa
     6601 gctggcctct gagggactga ctatgatcat cgtgacccat gagatggcct ttgcaagaga
     6661 tgtttccagc caggtcgtat ttatggcggg aggcgtgatc gtggaggaag gcgaaccggc
     6721 acagattttc ggagctccga aggagcatca gacgaaagag tttctggggc ggtttatgca
     6781 tgagagagcc gctttataac tgaatagtaa agagaaagcc cggatgaagg gcagccttag
     6841 gctgtctgtt cgtccgggct ttttggctgt catccctatg gaaattacct ggcctttgtg
     6901 atgtagggat aaagctcttc ataagcctct ctctgggcct catcctcagg aagagccggg
     6961 atcagacccg tacagtccat ggaagaacag gcctgaatat caatgtcata attgttttta
     7021 tggacctgag tttctgaatg tctgccggct ggatgtcttg tttgctccat ttcttgtcac
     7081 ctcctgacac gcatcaccat gttcttttga tagtgtggat ttaagagcgg tttttatgcg
     7141 gaaaggcaga aagataaggt ggaaaaatgg ggaagaaacg gggaaaaatg tttatggaag
     7201 cgtaagcaaa atgtaaaaaa gacaggggga ttttatgata tagtaagagt aggaatacat
     7261 ggggaggtac ggcatatgat ggaatatttt tttatgggcg gatttgagat catgtttttg
     7321 atggtatttg tgcttatttt cgggatgatc cttgtgatga tcgtgcgtgg aatcggagag
     7381 tgggggaaga ataaccggtc tcccaggctg gacgtatctg caacggttgt ttccaaacgt
     7441 acacatacca tgagaaatca aaaccataca agctcttcca gctactatgt tacctttcag
     7501 gtggaaagcg gagaccggat ggaactcagg ctgggcggaa gagagtatgg gcttttggca
     7561 gagggagata agggaaagct tcattttcag ggaacgagat atctggggtt tgaccggaac
     7621 tggtagagcg gggaaatgga tatggatgag gaaaatgacc catcttaagg gaaattttat
     7681 acttctgctc cttgatttat ggatggactg ccactataat agaaaagaat gggttttggc
     7741 acgttttatg cgtgtgaagg cagcctgttc agagggagaa atagcacagt atggataata
     7801 acaagaagca aaaccagaat atgaacagta actggcagac cattgccatg ctggtggtgg
     7861 ctgctctgct gacgatcatg atcgtgagct ggatgaattc cgcagtggaa tccagacagc
     7921 gcaaggaact gtcatacaat gaatttgtac agatggtgga agacggcaaa gtggaatcca
     7981 tccgggtaaa ggatgctgtt attgaagtaa tgccaaaggc agaggcaacg gaatattccc
     8041 agcagatgaa atactatgta gtccgtatag aaggggatta ccagttttta aatcgtgtgc
     8101 tggagaacaa tgttataacc aatcgggaaa atggcagtac caacgcactt cttatcgctc
     8161 tgcttaacta tgcagtaccg ttcctgatcc ttttgttcct tatgaacttc accatgaagc
     8221 gcatgggcgg aggcggcatt atgggcgttg gaaagagcaa tgccaagatg tatgtccaga
     8281 aggaaaccgg cattaccttt aaggatgtgg ccggagagga cgaggcgaag gagtccctga
     8341 cagaaattgt agatttcctt cacaatcccg gaaagtacac aaagatcggt gcaaagcttc
     8401 caaagggagc actgctggtg gggcctccag gaacgggtaa aacccttctg gcaaaggcgg
     8461 tggcaggaga agcacatgtg cccttttact ccctgtccgg ttccgatttt gtggaaatgt
     8521 ttgtgggcgt gggtgcgtcc cgtgtccgtg acctgtttaa aaatgcgaca gaaaatgcgc
     8581 cgtgtatcat ctttatcgac gagattgacg ctatcggccg cagccgcgac agccgcatgg
     8641 gcggaaatga tgagagggag cagaccttaa accagcttct ttctgagatg gacggctttg
     8701 attctacaaa gggacttctt gttttaggag ctaccaaccg gcctgagatc ctggatcccg
     8761 cgctccttcg tcccggacgt tttgaccgcc gggtagtagt agataagccg gatctgaacg
     8821 gaagaatcaa tattttaaag gtacattcca aggaagtgct tttggatgaa accgttgatt
     8881 tcaaggagat tgctctggct acttctggcg ctgtgggcgc tgatcttgca aacatgatga
     8941 atgaagctgc tatcaatgca gttaaaaacg gcagaagtgc tgtttcacag aaggatctct
     9001 ttgaggctgt ggaagtggtt ctggtcggta aggaaaagaa agaccggatc atgagcaagg
     9061 aggagcggcg gatcgtatcg tatcacgagg taggacacgc tctggtaagc gccctgcaga
     9121 agcattcgga gcctgtacag aagatcacca ttgttccaag aaccatgggt gcattgggat
     9181 atgtaatgaa tgttccggaa gaagagaagt tcttaaatac caagaaggag ctggaggccc
     9241 gtctggtaga gctcatgggc ggccgcgcgg cagaggagat tgtatttgag actgttacta
     9301 ccggggcttc caatgatatc cagcaggcca ccaatctggc aagagccatg gtaacccagt
     9361 atggtatgtc tgagaaattc ggcctcatgg ccttagaaag ccaggagaac cagtatctga
     9421 ccggccgtgc catattaaac tgcggtgacg ccacagcggc tgagattgac aaggaagtga
     9481 tgaaggtctt aaaggacgct tatgaagcgg caaagagcct gctcaatgag aaccgtgacg
     9541 ccatggatca gatcgcagac ttcctgattg aaaaggaaac catcaccgga aaagagttta
     9601 tgaagatatt ccgcaaggta aagggaatcc cggagcctga ggaaacgccg gaggaggaga
     9661 atggactgcc
//
LOCUS       DBMDUH010000102         9598 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_3377_length_9598_cov_6.381278, whole genome shotgun sequence.
ACCESSION   DBMDUH010000102 DBMDUH010000000
VERSION     DBMDUH010000102.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 9598)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9598)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9598
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3377_length_9598_cov_6.381278"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1488
                     /locus_tag="ACZPCG_09170"
     CDS             <1..1488
                     /locus_tag="ACZPCG_09170"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HIR1285261.1"
                     /translation="IADGSHQWENRLENGIWTYSMEAVWKGMQECYGDLKTKVREAYG
                     AEIETIGGIGISAMMHGYLAFDGNDELLVPFRTWRNTITEQASRELTECFNYPIPQRW
                     SIAHLYQAVLNGEAHVKDIKYITTLAGYVHWMLTGERVLGVDDASGMFPVDAKTGTYY
                     TDMLDCFDQKISDRSFPWKIREILPRPLLAGEQAGVLTEEGAGLLDPEGTLKAGIPLC
                     PPEGDAGTGMVATNSVKVRTGNVSAGTSIFSMIVLEKELSNVYSEIDLVATPQGAPAA
                     MVHCNNCTSDLNAWVNILKEFSDTFGFHISGDELYGGLFRKALEGDKDCGGLVACNYV
                     SGEPVAGFAEGRPLFVRRQDGRFNLANFIRTHLYAAFGTLKIGNDLLFDKEGVKVDVI
                     WGHGGIFKTEGTAQKILAAALNTPVSVMKSAGEGGAWGIAVLALYMINKKEGEALEDY
                     LEKRIFQKAESVTAAPEPEDIEGFNQFMENYKKGLMIEKAAVDAL"
     gene            complement(1548..2039)
                     /locus_tag="ACZPCG_09175"
     CDS             complement(1548..2039)
                     /locus_tag="ACZPCG_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003023158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HIR1285262.1"
                     /translation="MNRKKTDFLVHGALIAAVYVALTMMFAPISFGPVQFRISEALCI
                     LPFFTPAAVPGLFLGCLISNLLGTAVLMDVICGSIATLIGATGSYALRHHKRLVCLPP
                     ILANTLIIPWVLRYAYGSPDLIPFMMLTVGIGEILAVGVLGNGLLAILERYKYTIFRQ
                     SRA"
     gene            2519..3904
                     /gene="murC"
                     /locus_tag="ACZPCG_09180"
     CDS             2519..3904
                     /gene="murC"
                     /locus_tag="ACZPCG_09180"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003504779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HIR1285263.1"
                     /translation="MYQIDFDKPEHIHFIGIGGISMSGLAEILLDAGFTVSGSDAHET
                     ELTRHLTEKGARIYYGQRAANIEQEPGIQAVVYTAAIHGDNPELMAVRAKGLPELTRA
                     ELLGELMRNYKQSIAIAGTHGKTTTTSMLTDILLAGGTDPTISVGGILKDIGGNIRVG
                     GPEFFVTEACEYTNSFLSFYPTIEVILNIEEDHLDFFKDIADIRHSFRLFAEKLPAGG
                     LLVINKDIDRVEEITRGLNCRVITFGKDPSSDYTAGNITYDELGRPSYDLIVQGEVVN
                     RISLGVTGEHNVYNSLSAIAVALELGVGFDGVIAGLRKFTGTDRRFEKKGEIGGVTVI
                     DDYAHHPQEISATLKAAENYPHKRLWVVFQPHTYTRTKAFLDEFAQALSGADEVILAD
                     IFAARETDTLGVSSRDIAERLEKLGTSVHYIPSFDEIETFILEHCIHGDLLITMGAGD
                     IVKVGEKLLGL"
     gene            4197..5273
                     /locus_tag="ACZPCG_09185"
     CDS             4197..5273
                     /locus_tag="ACZPCG_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003524260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HIR1285264.1"
                     /translation="MSMNWKDRWSIGATMVADAFIDEYMAGANGEYVKVYLYILRHQG
                     EELTVESIADALDHTESDVRRAVSYWEKLGILSGAENEKKQEGELSWAADPAASCAEA
                     KEETAASADRAPVYSAAQVNRLCGDEEFSQLLYIAQKYLNKVFTPRDCQVFAWLYEGL
                     GMSSELLEYLVEYCAQNGHSSVRYLETVAVSWHEKGIKTSQEARELGAAYSKDSFAVM
                     RAFGINNRKAAPPEQAMIDKWFKEYGFAREIVLEACSRTITAIHSPSFQYADRILRDW
                     KKAGVKKMEDVRVLDARRQALVQENGGDKEKRLQRYNSASKDGGGKKNSQNQFHNFKQ
                     RDTDYDALMLKQVKEWVGGGRPRT"
     gene            5319..6299
                     /locus_tag="ACZPCG_09190"
     CDS             5319..6299
                     /locus_tag="ACZPCG_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1285265.1"
                     /translation="MALSNSQYDAIMREYGRQQLENQHQLEARRNEIYRVIPAVRELE
                     AEIASRSVAGAKRLLAGDTGALAELREELADLREQKAILIRAKGYPEDYLTLHYRCPD
                     CEDTGWRQGRKCHCFLRAQMKLLYAQSNLDHVLDQENFSRLSYDYYDSSEIIPELGLT
                     NADYMRRVVAGCREFAADFDRKKENLLFTGSTGVGKTFLTNCIAKELIDSGHSVIYLS
                     AGDLFEVFSRNKFDYDTPEDMRDTYRYILDCDLLIIDDLGTELNNSFTSSQLFYCINE
                     RMNMSRSTIISTNLSLKQLRDSYTDRVTSRIMRYRIIPLYGRDIRVLKRG"
     gene            6392..7561
                     /locus_tag="ACZPCG_09195"
     CDS             6392..7561
                     /locus_tag="ACZPCG_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HIR1285266.1"
                     /translation="MLYEEKVIETIPVGPLGLIPLKSCTELGNKVDRYLVDWRRERES
                     EHKSTIAFTGYQRDSYIINASTPRFGSGEGKGIIEESVRGDDLYIMVDVCNYSLTYSL
                     FGMTNHMSPDDHFQDLKRIIAAAAGKARRINVIMPFLYESRQHKRSGRESLDCALGLK
                     ELVNMGVENIITFDAHDPRVQNAIHLKGFETVQPIYQFLKHLLKNEPDLKIDSDHMMV
                     ISPDEGGTGRAIYFANMLGLDMGMFYKRRDYTKIVNGRNPIVAHEFLGSSVEGKDVLI
                     VDDMISSGESMQDVAKELKRRKARKVFICSTFGLFTNGLARFDEYYEKGFIDRILTTN
                     LVYQTPDLLQRPYYINVDMSKYIALIIDNLNHDASLSELLTPTSRINRLLEQYRS"
     gene            7705..8820
                     /locus_tag="ACZPCG_09200"
     CDS             7705..8820
                     /locus_tag="ACZPCG_09200"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIR1285267.1"
                     /translation="MDGGKMEERVQNTISEEDWFRNKVYWLSFLFSLLVVWAHSLNGE
                     LFVTGEQELETVQQLEHLAASQLAQIAVPGFFMISAYLFYRNFQLNSLFSKWRSRSRT
                     ILLPYLLWNSLYYGAYAAATRIPAICQIIGKPPVPLTAGEFLKALLHYGYNPVFWYLF
                     QLILLILLAPVLYVLLKKNVRGLLFLLFLILCLWKGVSFPWLNLDALFYYSAAAFFAL
                     RREQLGNYLERRPAEFLKERWKHLFIQITILSFFFWLLQITGLPAAPLHGHPLHTVLT
                     RLWGVCTAVCLIRIIPLPPAADLIKNSFFLYAVHFPWVRLLNKTAALILPSTPASALF
                     MFMLMPVFIIIITCIISSSMKKNFPGTYFVLSGGRGR"
     gene            9198..>9598
                     /locus_tag="ACZPCG_09205"
     CDS             9198..>9598
                     /locus_tag="ACZPCG_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HIR1285268.1"
                     /translation="MGKEEKMSVNRDMNQEEKLKALDAALTQIEKAYGKGSVMKLGES
                     GVNMNIETVPTGSISLDIALGLGGVPKGRIIEVYGPESSGKTTVALHMVAEVQKRGGI
                     AGFIDAEHALDPVYARNIGVDIDNLYISQPD"
BASE COUNT         2616 a         1991 c         2567 g         2424 t
ORIGIN      
        1 atagcggatg gcagccacca gtgggaaaac aggctggaaa acgggatatg gacctattcg
       61 atggaagcag tctggaaggg aatgcaggag tgctatggcg atcttaaaac aaaggtaaga
      121 gaagcatatg gggcagagat agaaaccatt ggcggaattg gaatcagcgc catgatgcac
      181 ggttatctgg catttgatgg aaatgatgaa cttctggtac cattccgcac atggagaaat
      241 accataacgg agcaggcgtc acgggaactg acagaatgtt tcaattaccc tatcccccag
      301 cgctggagta tcgctcatct gtatcaggcg gtgctgaacg gagaagctca tgtaaaagat
      361 attaaataca tcaccacgct ggcaggctat gtgcactgga tgcttaccgg agaacgggta
      421 ctgggtgtgg atgatgcgtc gggaatgttt cctgttgatg caaaaacagg aacctattat
      481 acggatatgc tggactgttt tgaccaaaag atttcagacc ggtcgtttcc atggaaaatc
      541 agggaaatcc tgccccggcc tcttctggca ggggagcagg caggcgttct tacagaggag
      601 ggtgcagggc ttttagatcc agaaggaaca ttgaaggccg gaatccccct gtgccccccg
      661 gaaggagatg cgggaacagg gatggttgca acaaacagcg taaaggtgcg cacaggcaat
      721 gtatccgcag gaacctccat attttccatg attgtactgg aaaaagagct ttcaaatgta
      781 tattctgaaa ttgaccttgt ggcaacgcct cagggggctc cggcggctat ggtacactgc
      841 aacaactgca cctcagacct gaacgcctgg gtgaatattc tgaaggaatt cagcgatacc
      901 tttggctttc atataagcgg ggatgagctg tatggagggc ttttccgaaa ggctctggag
      961 ggcgataagg actgcggagg actggtggcc tgcaattatg tttcaggaga accggttgcc
     1021 ggctttgcag aaggccgtcc gttgtttgtg cgccggcagg acggccggtt taatctggca
     1081 aattttataa ggacccattt atatgcagcc tttggtactc ttaagatcgg aaatgacctg
     1141 ctgtttgaca aagagggcgt gaaggtagac gtgatatggg gccatggagg catctttaaa
     1201 acggagggaa cggctcagaa aatccttgcg gccgctttga acactcccgt atctgttatg
     1261 aaatcagccg gagaaggggg cgcctggggg attgcagttc tggcactgta tatgataaat
     1321 aaaaaagaag gggaagcttt agaggattat ctggaaaaac ggatttttca aaaggcagag
     1381 agcgtaacag cagctccgga accggaagat atagagggct ttaatcagtt tatggaaaat
     1441 tataaaaaag ggctgatgat tgaaaaagcg gctgtagatg cattataaat aataaaaatg
     1501 ccgacacctt aacctgtaaa attacaagct aaggcgtcgg cattctttta tgctctgctc
     1561 tggcggaaaa tcgtatattt ataacgctcc agtatagcta agaggccatt tcccagaacc
     1621 ccaacggcca aaatctcgcc aatccctacg gtgagcatca taaaagggat cagatctggg
     1681 gaaccgtagg cataccgcag cacccagggg atgatcaggg tatttgccag gatagggggg
     1741 aggcatacca gcctcttgtg atgccgaagg gcataggagc ctgttgcacc gattagtgtg
     1801 gcaatactgc cacagatcac atccatgagc acggctgtac ctaagaggtt agagatcagg
     1861 catcctaaaa agagtcccgg aacggctgcc ggtgtaaaga agggaaggat gcacagcgct
     1921 tcagagatgc gaaactgcac cggtccgaag ctgatgggag cgaacatcat agtcagcgcc
     1981 acatacacgg ctgcgatcaa tgcaccgtgt accagaaaat ctgttttttt cctgttcata
     2041 tttagttgtc tcctttagtt ttgttttacg agtggaaggt ctcgaacact cgattcattt
     2101 tacgccggaa atcggcggaa agccccttta gaccttattt ttgggcttta cagcgtctcc
     2161 tagtttagca tgatttgcag agcaggtcaa gaggaatgtt ttagtaacag ttcagcatta
     2221 catgttttat ggtattctca cagcaaacta tgttttttaa tatgtttatg ataaaatagg
     2281 atgaacaaag gcagcagtat cttcatcctt tgggattgct gctgtgccgg agcatgacat
     2341 ttaaagatct ggccgataag gcggatcaga tgctgtatga aataaaacgc ggcagtaaaa
     2401 aggctatcgg gtatatggaa tgcttttata atttcatatg acatttccgg cggactttga
     2461 tataattata aggaaagcag acaaatacat accgtatcac agggaaaggg aataatatat
     2521 gtatcagata gattttgaca aaccggaaca cattcacttt attgggatcg gaggcatcag
     2581 tatgagcgga ctggcagaga tcctgctgga cgccggattt accgtatccg gctctgacgc
     2641 ccatgaaacg gagcttacca gacatctgac agagaagggg gcgcggattt attacggcca
     2701 gcgggccgct aatattgaac aggagccggg gattcaggcc gtggtttaca cagcggcgat
     2761 ccatggcgac aacccggagc ttatggcagt gcgggcaaag gggcttccgg agcttaccag
     2821 agcggagctt ttaggggaac tgatgcgtaa ttataagcag tccatagcca tagccggtac
     2881 gcacggaaaa acgaccacta cctctatgct gacagatatt ctgttagccg gcggcacaga
     2941 ccctacgatt tctgtaggcg gtatcctgaa ggatatcggc ggaaatatac gggtaggcgg
     3001 gcctgaattc tttgtcacag aggcctgtga atatacaaac agctttttat ccttttatcc
     3061 caccattgag gtgattttaa atattgaaga ggatcatctg gatttcttta aggatatcgc
     3121 ggatatccgt cattccttcc gtctgtttgc agagaagctt ccggcaggag gccttctggt
     3181 gatcaacaaa gatattgacc gggtggagga aattacccgg ggactgaact gccgcgtaat
     3241 caccttcgga aaggatccgt ccagtgatta tacagccgga aacattacat atgacgaact
     3301 tggcagaccg tcctatgacc tgattgtgca gggagaggta gtaaaccgga tttctctggg
     3361 agtaacaggg gagcataacg tatacaattc tctgtcagcc attgctgtcg ctctggaatt
     3421 aggcgtaggc tttgacggcg taattgcagg tctcagaaaa tttacgggaa cagaccggcg
     3481 gtttgagaaa aagggagaga tcggcggagt aacggtgatt gatgattatg cccatcatcc
     3541 acaggagatc agtgctactc tgaaggcggc agagaattat ccccataagc gcctctgggt
     3601 agtatttcag ccccatacct atacccgtac aaaggcattt ctggatgaat ttgctcaggc
     3661 cctgtcaggt gctgatgagg tgatactggc agatatcttt gcagccagag agacggatac
     3721 actgggagtc agctcaaggg atattgcaga acggctggaa aagctgggta caagcgtcca
     3781 ttatattccg tcctttgatg agattgaaac atttattctg gaacattgta tacacgggga
     3841 tctgttgata actatgggtg ccggagatat tgtaaaagtg ggagaaaagc tccttgggct
     3901 gtagttatcc acaatatcca catagttttc aacacgaaac agagctaaaa actgtggatg
     3961 aatcatctga tatgaaagga tgataggaga tttggaaaac cacggtttta tgcggaaaat
     4021 tgacagctta tgggggaatg agaccagatg ggggatggtt tttccacaat atccacatta
     4081 tccacagaat gagcagtgga aagaaggcac tattttcttt tagcagtgga tatgatatac
     4141 tgtctgcaaa catttcccgg acagaaaaag ggaaaatgca gcaaggagtg gtgaaaatga
     4201 gtatgaactg gaaggaccgc tggagcatag gggctaccat ggtggcagat gcgtttatag
     4261 atgaatatat ggccggtgcc aatggggaat atgtaaaagt atatctttat attctgcgtc
     4321 atcagggcga agagcttacc gtggagtcta tagcggatgc tctcgatcat acggaatccg
     4381 atgtgcggcg tgctgtaagc tattgggaaa aactggggat tctttccggg gcagaaaatg
     4441 agaaaaagca ggaaggggag ctttcttggg ctgcggatcc ggctgcttcc tgcgcagagg
     4501 ctaaggagga aacggctgcc agcgcagacc gtgctccggt gtattctgcg gctcaggtaa
     4561 accggctttg cggagatgag gagttttccc agcttcttta tattgcgcag aaatatttaa
     4621 ataaagtttt tacaccaaga gactgtcagg tatttgcatg gctctatgag ggacttggca
     4681 tgagcagtga gctcctggaa tatcttgtgg aatactgtgc ccagaacggc cacagttcgg
     4741 ttcggtatct ggagacggta gcagtgagct ggcatgaaaa agggattaaa acatctcagg
     4801 aggcacggga gctgggagca gcttattcga aagattcctt tgcggtcatg cgggcctttg
     4861 gaattaacaa ccgcaaggca gcgcctcctg agcaggccat gatcgacaaa tggtttaagg
     4921 agtacgggtt tgccagagaa atcgttttag aggcctgcag ccggaccatt accgccatcc
     4981 acagccccag ctttcagtat gcagaccgta ttttaagaga ctggaagaaa gccggcgtaa
     5041 agaaaatgga ggatgtccgt gttttggatg ccagacgtca ggctctggta caggaaaatg
     5101 gcggagataa ggaaaagcgg cttcagcgct ataacagcgc atcaaaggat gggggaggga
     5161 aaaagaactc tcaaaaccag ttccataatt ttaaacagag ggatacagat tacgatgccc
     5221 tgatgttgaa acaggtaaag gaatgggtgg gcggaggaag accccgtaca taaggaaccg
     5281 ccccgggagg gcgttacaga ctggaaggag aacaggagat ggcactgagt aattcccagt
     5341 acgacgcaat catgcgggaa tatggcaggc agcagcttga aaaccagcat cagctggaag
     5401 cacgaagaaa tgaaatttac cgggtgattc cggcagtccg ggaactggaa gcggagattg
     5461 ccagccgttc tgtggccggg gcaaagcggc ttttggccgg ggatacggga gcgttagcgg
     5521 agcttagaga agaactggca gatttaaggg agcagaaggc tattttaatt cgcgccaaag
     5581 gatatccgga ggactatctt acgcttcatt acaggtgccc ggactgtgag gatacaggct
     5641 ggcgccaggg ccgcaaatgt cactgctttc tcagggctca gatgaagctg ctctatgccc
     5701 agtccaatct ggatcatgtg ctggatcagg aaaatttttc cagattatcg tatgactatt
     5761 atgacagcag tgaaatcatt ccggagcttg ggctgaccaa tgcagattat atgcgcaggg
     5821 tggtggcagg ctgtcgggaa tttgctgcag attttgaccg aaaaaaggag aatctactct
     5881 ttacaggcag tacaggagtg gggaaaacct ttcttacaaa ctgtattgca aaggagctga
     5941 ttgattcagg ccattcggtg atctatctgt cggcaggcga tctgtttgag gtgttttcca
     6001 gaaataaatt cgactatgat accccggagg atatgaggga cacatatcgc tatattctgg
     6061 actgcgacct gctgatcatt gacgatctgg gaacggagct caataacagc tttacctctt
     6121 cgcagctttt ttactgcatc aatgagcgta tgaatatgag ccgttccacc attatttcca
     6181 ccaatctgtc tttaaagcag cttcgggatt cctacacgga tcgtgtgacc tcccggatca
     6241 tgcgctatcg gatcattccc ctttacggca gggatatccg tgttttaaag aggggatagc
     6301 ttgacgaaat gcatgcaaag atggtataaa gataataatg aacggcaggt gcgtttttgg
     6361 cgcgcagtac aaacaggagg ataaagaaat catgttatat gaagagaaag tcattgagac
     6421 cattccggtg ggccctcttg ggttgattcc cttaaagagc tgtacggagc tgggaaataa
     6481 ggtggacagg tatctggtag actggagaag agagcgggag agcgagcata aatctaccat
     6541 cgctttcacc ggttatcaga gagattctta tattatcaat gcatctaccc cccgctttgg
     6601 ttccggcgaa ggaaagggaa ttattgagga atccgtaaga ggcgatgacc tttatatcat
     6661 ggtggatgta tgtaattaca gtctgaccta ttcccttttc ggaatgacaa accatatgtc
     6721 tccggatgat cattttcagg atttaaagcg tatcatcgca gctgcagcag gaaaggcacg
     6781 caggatcaat gtgatcatgc cattcctcta tgagagccgc cagcacaagc gttccggccg
     6841 tgagtcctta gactgtgctc tgggactgaa ggagcttgta aatatgggcg tggaaaatat
     6901 tattaccttt gacgcccacg atcccagagt tcagaatgca atccatttaa agggctttga
     6961 gacggtacag ccgatctacc agttcttaaa gcatctgctt aaaaatgagc ctgatttaaa
     7021 gatcgacagc gatcatatga tggtaatcag cccagatgag ggcggtactg gccgcgctat
     7081 ttatttcgca aatatgctgg gactggatat gggtatgttc tacaagcgtc gcgactacac
     7141 gaagatcgta aacggaagaa atcccatcgt ggctcatgag tttttaggct ccagcgtaga
     7201 gggaaaggat gttctgatcg ttgacgatat gatctcctcc ggcgagagca tgcaggatgt
     7261 agccaaggaa ttaaaacgca gaaaagccag aaaggtattt atctgctcta cctttggcct
     7321 gtttacaaac ggacttgcca gatttgacga atattatgaa aagggcttta ttgaccggat
     7381 cctgacaacc aatctggtat atcagacgcc ggatctttta cagcgtcctt actatatcaa
     7441 tgtagatatg agcaagtata ttgcccttat cattgacaat ctgaaccatg atgcttcctt
     7501 aagcgagctt ctcacaccta ccagccgtat taaccgtctg ctggagcagt atagaagctg
     7561 atttctaagg cggcaatgtt taggggctgt ttcctgcggg aagcggccct ttttggttct
     7621 gtatctgaat ttcatctatt tgaagcagat acaggagctg actttgatcg acttttgtac
     7681 acacctttag gaggctgcgg gaaaatggat ggtgggaaaa tggaggaacg ggttcaaaac
     7741 acaatttcag aagaagactg gttcagaaat aaggtgtatt ggctcagttt tctgttcagt
     7801 cttctggtcg tatgggccca ctctttaaat ggagaacttt ttgtaacagg ggaacaggaa
     7861 ctggaaacgg tacagcagct ggaacatctg gcagccagtc agctggctca gattgcagtg
     7921 ccgggttttt ttatgatatc tgcgtattta ttttacagaa atttccagct aaacagcctg
     7981 ttttccaaat ggagatcccg gtcaagaaca atcctgcttc cctatcttct ctggaatagt
     8041 ttgtactatg gggcttatgc tgcggcaacc aggattcccg ccatctgtca gatcattgga
     8101 aagccgccgg ttccgcttac tgccggggag tttcttaaag ctctgctgca ctatggttat
     8161 aatcctgtgt tctggtatct gtttcagctg atcctgctta ttctcctggc tccggttttg
     8221 tatgttcttt tgaaaaaaaa cgtaagaggt ctcctttttc tgctcttttt gatcctgtgt
     8281 ctatggaagg gagtgtcgtt tccctggctt aatctggatg ctctttttta ttattctgcg
     8341 gcagcttttt ttgctttgag aagggaacag ctgggaaatt atctggagcg ccggcctgcg
     8401 gagtttctta aggaacggtg gaagcactta ttcatccaga tcacaattct gtcgtttttc
     8461 ttttggctgc ttcagattac aggccttccg gctgctcctc tccatggcca tcctcttcac
     8521 acggttctta cacgcctgtg gggggtatgc acggcggtat gcctgatccg catcattccc
     8581 cttccgcctg ccgcagacct cataaaaaac agcttctttc tttatgcggt tcattttccc
     8641 tgggtccgcc tgctcaataa aacggcggcc ctcattctgc cctccacacc tgccagcgct
     8701 ctttttatgt tcatgctgat gcctgttttt atcattatca tcacctgtat cataagcagc
     8761 tcaatgaaaa aaaattttcc gggaacctat tttgtccttt ctggaggcag aggaagatga
     8821 aagggagatg aaaggaaaac ttccggtcgg gaagaccctt gcaaaaacag gaaatctatg
     8881 ctattctgaa cctgcagcac aggccatctg tcctgtgctg cagcgtcatt tcacagcttt
     8941 tccagtcttt ggatggagaa accatccgtg tcagggttct tcgcccggat ttaccataca
     9001 gcacgcagcc gtacattctg ccgtgcagaa tcagatcact ctccccggta ttctgcacca
     9061 gaacgtaata atacacccta aaaatccgtt gacatttgga tggttctata ctatgattat
     9121 attaaaagaa catttgttcg gaacgaagcg tcgtctccgg tttcagacgg aaagagagat
     9181 ttcattagga ggaaaatatg ggcaaggaag aaaagatgag cgtaaacaga gatatgaatc
     9241 aggaagaaaa gttaaaggcc ctggatgcgg ccctcacaca gattgaaaaa gcctatggca
     9301 agggttctgt gatgaagctg ggggaatcag gtgtgaatat gaatatagaa acggttccca
     9361 caggttcgat cagtctggat attgcactgg gattgggcgg tgtccccaag ggaaggatta
     9421 tcgaggtata cggtccggag tcttccggta agaccacggt tgcgctccat atggtggcag
     9481 aagtacagaa acggggcggt attgcgggct ttattgacgc ggagcatgca ttggatccgg
     9541 tttatgcccg gaatattggc gtagatattg ataatcttta tatttctcag ccggataa
//
LOCUS       DBMDUH010000103         9581 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_3385_length_9581_cov_4.465717, whole genome shotgun sequence.
ACCESSION   DBMDUH010000103 DBMDUH010000000
VERSION     DBMDUH010000103.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 9581)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9581)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9581
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3385_length_9581_cov_4.465717"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="ACZPCG_09210"
     CDS             complement(<1..554)
                     /locus_tag="ACZPCG_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIR1285269.1"
                     /translation="MTYKIIGDSCLDLTEELKKDPGFQMIPLTLQVGNVQVIDDETFD
                     QSAFIEMVKACPECPKTACPSPESFKKAYEEADVDAVFVITLSNHLSGSYNSAVLAKE
                     LYEEEKKEAGETVTKKIAVIDSLSASSGELDQALYIRDLCEQGLDFETVAEMAEAYSH
                     RMKTYFVLETLDTLRKNGRLSGLQA"
     gene            complement(681..2102)
                     /locus_tag="ACZPCG_09215"
     CDS             complement(681..2102)
                     /locus_tag="ACZPCG_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285270.1"
                     /translation="MKTIQGVCLGAALSLSGAFGVFAEPLGLSTIDGQIITSRMGGFQ
                     AQVPASYQVDRMGGGWEISRQLVAQMKTSDQMQIDFAASMVNETDKTYIVNMGFVMPA
                     DDGTGIPFSVETLKAEMIQSGLDETMTQSGMITLGTKEYEYLKVNYGKLMADSMKDYM
                     NTAELTEGQKATVQNYISQMEKMLVNDFYIRQEGDRIYVICQTYSADQAAEAAAFLDC
                     LQPYSGIEGWEYSEDGGWKYRLADGSFAKDCWKQDEFGLTYRLDGEGKILYSSWIKEA
                     DGWKYVDEYGHMVTSVTKSIDGVEYTFGADGYMKEGSERAAAEFTLGTLEGNHYRNEW
                     ADLELTFPEAATVLKGDGSSRTYALVGGEHVDVDDPELSYRVTLDFIDAEAELDRYME
                     ALREYESTGGYQVDRFETVELGGHTWCSCLSSTRFSDGTSHHSDWYVRQIEGRFVILH
                     FDYYEELKSQADQVYQSIRAAGR"
     gene            complement(2231..4240)
                     /gene="htpG"
                     /locus_tag="ACZPCG_09220"
     CDS             complement(2231..4240)
                     /gene="htpG"
                     /locus_tag="ACZPCG_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HIR1285271.1"
                     /translation="MAKKGSLSITSENIFPVIKKWLYSDHDIFYRELISNGCDAITKL
                     KKLELMGEYEKPEDVEYKIQVSVNPNEKTITIRDNGLGMTEEEIDKYINQIAFSGVQD
                     FMEKYKDKANEDQIIGHFGLGFYSAFMVADKVTIDSLSYQKDAKPVHWESEGGIDYEM
                     TEGEKAEVGTTIVLYLNEDSTEFSNEYRAREVIEKYCAFMPVSIFLDNETAEPQYDTI
                     EKDELTEKDTVVETVIEPAKTEEKEKEDGTKETVEIEPSREKYKILRRPVALNDTNPL
                     WNKHPNECTEEQYKDFYRKVFRDYKEPLFWIHLNMDYPFNLKGILYFPKINMEYESIE
                     GTIKLYNNQVFIADNIKEVIPEFLMLLKGVIDCPDLPLNVSRSALQNDGFVKKISDYI
                     TKKVADKLSGMCKTDRETYEKYWDDIAPFIKFGYIKDEKFADKMGDYILYKNLEGKYL
                     TLQDCLDENKEKHENTIFYVTDEKEQSQYINLFKEEGIDAVIMPAAIDNPFISHVEQK
                     KEGVKFLRIDTDLNASFKEEVKEDDEEFKKSSEELTEVFKKALNNDKLDIKVEKMKNA
                     GIASMITVSEETRRMQDMMKMYNMGGMDMGMFGGMGETLVLNANHPLVKYVLENKEGE
                     HTAAICEQLYDLASLSHGALSPERMTKFVSRSNEIMMIMAGQKTE"
     gene            complement(4426..5637)
                     /locus_tag="ACZPCG_09225"
     CDS             complement(4426..5637)
                     /locus_tag="ACZPCG_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR1285272.1"
                     /translation="MDKTKLMKKLDELTALAAAHDKRLEMTQVLDCFADEAVTPEEME
                     DVYARLEKRGVQILAEEGTDTSEDEGLLLEEDGDDSEDTVSELINSKYRWNREDSETD
                     DEDFSPSADNDEEDEENSHEQLLEGVASTDPIREYLKEIGSIPLLTQEQEQELAKRKA
                     LGDLHAGQKLVEANLRLVVSIAKRYTGRGMSFLDLVQEGNIGLMKAVEKFDYTKGYRL
                     STYATWWVKQSVTRALADQSRTIRLPVHMVEAVNRVRRAQRMLAVRLGREPSNEEIGK
                     EIGMSEKRVTELMQSSGDTVSLETPVGDEDGSNLGDFVADDANASTEEKAESVFLREE
                     IDQMLQGLNPREREVIILRFGLESGHPMTLEEVGKRFKVTRERIRQIETAALRKLRNP
                     SRSRKIRDFLP"
     gene            complement(5697..6203)
                     /locus_tag="ACZPCG_09230"
     CDS             complement(5697..6203)
                     /locus_tag="ACZPCG_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5721 family protein"
                     /protein_id="HIR1285273.1"
                     /translation="MIALKAEDVKSFTSRLFIKEDFDSFWVREVNIVTYNSFTMDGHI
                     RSGYYTEEEREEQRIGEFSSWRQLRPFCFSLIKGKKLPESFKIVLQLPAGQTERFASK
                     SGTGISGDQIQGLYLNIRYEEGELYCITGTALKFFTLDKALEVQWDESVREFFRSRQI
                     PCTQEGVN"
     gene            complement(6407..7564)
                     /locus_tag="ACZPCG_09235"
     CDS             complement(6407..7564)
                     /locus_tag="ACZPCG_09235"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003526478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0030388 - fructose 1,6-bisphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HIR1285274.1"
                     /translation="MSLIPLRPLLEASMKYGFGQGAFNVNAVAQAKAAIEVHEMFRSA
                     AILQGADLANGFMGGNTDFIHATLEDKKIGARNIAGAVKKYAQTAAIPVVLHLDHGRD
                     FDSCVAAIEGGYTSVMIDGSSLPFDENVELTREVVKYAHARGVSVEGELGVLAGVEDH
                     VFAASSTYTNPLSAVEFFQKTGVDALAISYGTMHGASKGKNVKLRKEIPVAIRECMNH
                     LGIFGALVSHGSSTVPGYIVDEINALGGELADTHGISIEQLKAAIRCGINKINVDTDI
                     RLAVTRNMKELFAARPKLRTSPSVGGIYELLEKKKDQFDPRVFLPPIMDTVMYGVVED
                     EDRAAIVDCIERGVKEVVGTLIVEFGSFGKAPLVEQVSLEEMAERYRKAGI"
     gene            complement(7555..9006)
                     /locus_tag="ACZPCG_09240"
     CDS             complement(7555..9006)
                     /locus_tag="ACZPCG_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007887244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HIR1285275.1"
                     /translation="MKVSFEYGQGMVDAVLPDSTDIFIPGETVPDPPCIPEDKLEEET
                     LKSIRNPMGMPPLTELAHKGSKVVIIFPDIVKGGLQPTAHRKISIKLILQELYSVGVE
                     KKDILLICSNGLHPRSTEKEIRDILGEELFHEFWYTHQIISHDSEDYEHMVDLGTTDR
                     GDPVLMNKYVYDSDVAILIGHTQGNPYGGYSGGYKHCSTGITHWRSIASHHVPQVMHR
                     KDFTPVNGHSLMRTKFDEIGQHMEKCMGKKFFCCDAVLDTYSRQIEINSGYAKVMQPH
                     SWITADKRTYIPWAEKKYDVMIFGLPQKFHYGDGMGTNPVMMMQAISAQVVRHKRVMS
                     DHCVIICASICNGYFHDERWPYMRELYDMFQKDYMNILPDLNRYGEYFATNEEYIRKY
                     RYCNAFHPFHGFSMMSSAHIAEMNTSAIYVCGAQEPGYARGMGLKTRATIEEALEDAK
                     KKFVGPNPNILALPKTFKLGAVHLMMKEGDPCL"
     gene            complement(9032..>9581)
                     /locus_tag="ACZPCG_09245"
     CDS             complement(9032..>9581)
                     /locus_tag="ACZPCG_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004223396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HIR1285276.1"
                     /translation="TANAARALGCSYVLIGHCEERKDKAGILAAGGGAPAAVNGILNQ
                     EIREAVRAGLKVLYCIGETSEEQPRWQEVLRTQLEEGLKDVDKSCVTIAYEPVWAIGP
                     GKIPPDKEYIQKIGAFVKEITGDMDVVYGGGLKQDNAAMLASVPDMDGGLIALTRFSG
                     EIGFYPEEYLEIVKLYLTAEKA"
BASE COUNT         2244 a         2691 c         1900 g         2746 t
ORIGIN      
        1 gcctgaagcc cggacagacg gccgttctta cgcagcgtat ccagagtctc cagtacgaag
       61 taggtcttca tcctgtggct gtaagcctct gccatttctg caactgtctc aaaatccagc
      121 ccctgttcac acaaatcccg gatataaagc gcctgatcca gttctcctga ggaggcagaa
      181 agggaatcga tcacagcaat cttctttgtc accgtctcac cggcttcctt cttttcctcc
      241 tcatacagct cctttgccag cacggcagaa ttatagctgc cgctcagatg gttggaaagg
      301 gtgatcacaa atactgcgtc cacatctgcc tcctcatatg cctttttaaa gctctccggc
      361 gaagggcagg ccgtcttggg acactccggg caggccttta ccatctctat gaatgcgctc
      421 tgatcaaagg tttcgtcatc tatcacctga acattcccca cctgaagggt caggggaatc
      481 atctgaaatc ccggatcctt ttttaattcc tctgtcagat ccagacagct gtctccaatg
      541 atcttatacg tcataatcct atcctccaaa catactatgt catttcctca acacatagtt
      601 ttgattacta cttattataa cagcatactc tgcaaaatgc aatacccgga cacacagaac
      661 ggcggaatga gcggatcccc ttaacgcccc gcagccctga tgctctgata tacctggtct
      721 gcctggcttt tcagttcctc ataataatca aagtgaagga tcacaaacct gccctcaatc
      781 tggcgcacat accagtccga gtgatgggaa gtaccgtcag aaaaccgggt ggaacttaag
      841 catgaacacc aggtatggcc tcccagctcc acggtttcaa aacgatccac ctgataccct
      901 cctgtgcttt cgtattccct cagcgcctcc atataccggt caagttctgc ttccgcatct
      961 ataaaatcaa gtgtcacgcg ataacttaat tccggatcat ccacatctac atgttctccg
     1021 cctacgagag catatgtcct gctgcttcca tcgcccttta aaaccgtcgc cgcctcaggg
     1081 aaggtcagct ccaggtctgc ccattcattc cggtaatgat tgccctccaa ggttcccagt
     1141 gtaaattctg cagcagcccg ttcactcccc tctttcatgt aaccgtctgc gccaaaggta
     1201 tactccaccc cgtcaatgct cttcgttaca gaagtaacca tatgaccgta ctcatccaca
     1261 tatttccagc catctgcttc tttaatccag ctgctgtaca agatctttcc ttctccgtcc
     1321 agacgatagg tcagtccaaa ctcatcctgc ttccagcagt cctttgcaaa gcttccgtct
     1381 gcaagacgat acttccagcc tccgtcttcg gaatactccc agccctcgat cccgctgtag
     1441 ggctgcagac aatcaagaaa tgcggctgcc tctgctgcct ggtctgcaga atacgtctgg
     1501 cagatcacat agatcctgtc tccttcctgg cggatgtaga aatcatttac cagcatcttt
     1561 tccatctggc tgatgtagtt ctgcactgtc gccttctgcc cctctgtgag ttctgccgta
     1621 ttcatataat ccttcatgga gtcggccatg agttttccat aattcacctt cagatattca
     1681 tattcctttg ttcccagggt aatcataccg ctctgggtca tggtctcatc cagaccggac
     1741 tgtatcatct ctgcttttaa ggtttccaca ctgaagggaa tccccgttcc atcatctgcc
     1801 ggcatcacaa atcccatatt tacaatataa gtcttatccg tctcatttac catggatgca
     1861 gcaaaatcaa tctgcatctg atccgatgtt ttcatctggg ctaccagctg cctggatatt
     1921 tcccagccgc ctcccatgcg gtctacctga taggaagccg gaacctgggc ctgaaaacca
     1981 cccatacggc tggtaatgat ctgcccatca atggtgctga gtcccaaagg ctctgcaaat
     2041 accccaaacg caccagaaag tgagagcgct gctcccaggc agacgccctg tattgtcttc
     2101 aaaaaacgtt tttttatcaa tgatctttcc tcccgtattc tccgtaaatc caaaaaagcc
     2161 gatgcagacc gctgcaccgg cttcccctgc agagcttctc acagcctctg ccgtattctt
     2221 attttactct ttattctgtc ttctgtccag ccataatcat cataatctca ttgctgcggc
     2281 tgacaaactt tgtcattcgt tcaggagaaa gggctccgtg ggaaagactt gccagatcgt
     2341 aaagctgctc acagatggcg gcagtatgtt ctccttcttt attttcaagc acatacttaa
     2401 ccagaggatg gttggcatta agcaccaggg tttctcccat tccgccgaac atgcccatat
     2461 ccattccgcc catgttgtac atcttcatca tatcctgcat tcttcttgtt tcttcggata
     2521 cggtgatcat ggatgcaatg cctgcattct tcatcttttc aaccttgata tccagcttat
     2581 cattatttaa agctttctta aatacctcgg tcagctcctc agaggacttc ttaaattcct
     2641 cgtcatcctc ttttacttcc tccttaaagg aagcgtttaa gtccgtatca atacgcagga
     2701 acttcacgcc ctctttcttc tgctccacat ggctgataaa cggattgtca atggcagccg
     2761 gcatgataac agcatcgatc ccttcctcct taaagaggtt gatatactgg ctctgctcct
     2821 tttcgtcggt tacatagaaa atggtatttt catgcttttc cttattctca tccaggcagt
     2881 cctgtaaggt cagatacttg ccctccaggt tcttatacag gatatagtcg cccatcttat
     2941 ctgcaaactt ctcatccttg atatagccga acttgatgaa gggagcaata tcatcccagt
     3001 atttctcata ggtctcacgg tctgtcttgc acataccgga cagcttgtcc gctaccttct
     3061 ttgtaatata atcggaaatc ttctttacaa agccgtcgtt ctgcagggcg cttctggata
     3121 cgtttaacgg aagatccgga cagtcgatca caccctttaa cagcattaag aactcaggga
     3181 ttacttcctt gatattatct gcgataaata cctggttatt gtacagctta atggttccct
     3241 cgatgctctc gtactccatg ttgatcttcg ggaagtacag aatacccttt aagttaaagg
     3301 gataatccat gttcaggtgg atccagaata atggctcctt gtaatctctg aataccttgc
     3361 ggtagaaatc cttatactgc tcctctgtac actcgttggg atgcttattc cacagaggat
     3421 tggtgtcatt taacgctaca ggacgcctta aaatcttata tttttctctg gacggctcga
     3481 tctccacggt ttccttggta ccgtcctcct tttccttctc ctcggtcttt gcaggctcga
     3541 taacagtctc aactaccgta tccttttcgg tcagttcgtc cttttcgatg gtatcatact
     3601 gaggctctgc ggtctcatta tccaggaaaa tgcttacagg catgaaagca cagtatttct
     3661 caataacctc tcttgcacgg tactcgttgg agaactcggt gctgtcctcg ttgaggtata
     3721 aaacaatggt cgtaccaacc tctgcctttt ctccctctgt catctcatag tcaatgccgc
     3781 cttcagactc ccagtgcacc ggctttgcat ccttctgata ggataaggaa tcaatggtaa
     3841 ccttatctgc caccataaat gcggaataaa agcccagacc gaaatgaccg atgatctggt
     3901 cttcatttgc cttgtccttg tacttctcca tgaagtcctg aacgccggaa aatgcgatct
     3961 ggttaatata tttgtcaatc tcttcctccg tcatgccaag gccattatcc ctgatggtaa
     4021 tggtcttttc attggggttt acgctgacct ggatcttata ctccacatcc tcaggctttt
     4081 cgtactcgcc catcagctcc agctttttaa gcttggtgat cgcgtcgcag ccattgctga
     4141 tcagctcgcg gtaaaagata tcgtgatcgg aataaagcca ctttttgatc acagggaaaa
     4201 tgttctcgct tgtaatagat aaactgcctt tctttgccat aatatgcgcc tctttctttt
     4261 gatttttctt ttctgaaact catatggtct gggacttcca aagtggttct ggaaatatcc
     4321 ctttatttta tctgctcata aattacattg tagtaccact ttggagaaat gtcaacaaaa
     4381 aattagcact cattcgaaat gagtgctagc aaaatctttt agtttctagg gaagaaaatc
     4441 ccttattttc ctgctcctgg acggatttct cagttttctt aaagccgccg tttcaatctg
     4501 gcggatacgt tctctggtaa ccttaaaccg ctttcccact tcctccagtg tcatgggatg
     4561 gcccgattcc aggccaaagc gcaggataat gacctcccgc tctctggggt ttagaccctg
     4621 gagcatctga tcgatttctt ctcttaaaaa tacgctctca gccttttcct ctgtggatgc
     4681 gttagcatcg tctgccacaa aatctcccag attggaaccg tcctcatcac ccaccggcgt
     4741 ctctaaagat actgtgtcgc cggaagactg catcagctct gtgaccctct tttccgacat
     4801 tcctatctct ttcccgatct cctcattgga aggttcccgg cccagccgca ctgccagcat
     4861 cctctgagcc ctgcgcaccc ggtttacagc ttctaccatg tgaacaggaa gacggatagt
     4921 tctggactga tccgccagcg ctcttgttac agactgtttt acccaccagg tagcgtaggt
     4981 gctgagacgg tagccctttg tataatcaaa cttttccacc gctttcatca ggccgatatt
     5041 tccctcctgg accagatcca gaaagctcat cccgcggcct gtatagcgct ttgcaatgct
     5101 gactaccagc ctgagattgg cctctaccag cttctggcct gcatgcaggt ctcctaaggc
     5161 cttgcgtttt gccaattcct gctcctgctc ctgggtaaga agaggaatgg agccgatttc
     5221 ctttaaatat tcccggatcg gatccgtact ggctacgccc tctaacagct gttcgtgaga
     5281 attttcctca tcttcctcat cattgtccgc agatggagaa aaatcctcat catctgtctc
     5341 ggaatcctcc ctgttccagc ggtatttgct gtttatcagt tctgatacgg tatcctcaga
     5401 atcatcccca tcttcttcca gaagaagtcc ttcatcctca gatgtatccg ttccctcttc
     5461 tgccagaatc tgtacgcccc gcttttccag tctggcatat acatcttcca tttcctccgg
     5521 cgttactgct tcatctgcaa agcagtctaa cacctgggtc atctcaagac gtttgtcatg
     5581 agctgctgcc aaagcagtca gttcatccag tttcttcatc agctttgtct tatccatatt
     5641 gctcccttcc ctgttttaag agcttgtccc tcatatgctt tcgatttttc gctctgctaa
     5701 tttacccctt cctgtgtgca cggaatctga cgggaacgga aaaactccct cacagactca
     5761 tcccactgaa cctccagggc cttgtccaga gtaaaaaatt taagtgcggt tcccgtaata
     5821 cagtataatt ccccttcttc ataccggata ttcagataca gtccctggat ctgatccccg
     5881 cttattccgg tgccgctttt cgaagcaaaa cgctctgtct gtccggccgg aagctgcagt
     5941 acgatcttaa agctttcagg cagctttttt cctttgataa gagaaaaaca aaacgggcgc
     6001 agctgtctcc aggaagaaaa ctccccgatc ctttgctcct cccgctcctc ttctgtataa
     6061 tacccggatc tgatatgtcc atccatggta aaactattgt atgtaacgat attcacctcc
     6121 cgcacccaga aggaatcaaa atcctccttt ataaacagtc gggatgtaaa ggactttacg
     6181 tcctcggcct ttaatgcgat cataagttct ctcctgtccg gcctattgtg tctaaatttt
     6241 ccaaatctta tctaattata cctttttagc cgaaaaacgt caatcagttt aaaggaagtg
     6301 tcgcaaaaat tgtcgcaaaa accgcttctg ttctctcagc tgtataaaat acggcagaga
     6361 gaaacgcttc tctctgccgc agcatcagct cttttaacct gtacctttaa attcccgctt
     6421 tccgatagcg ctctgccatt tcttccagag atacctgctc cacaagaggc gcttttccaa
     6481 aggagccaaa ctctacgatc agagtcccca caacctcttt cacaccccgt tcgatacagt
     6541 ccacaatggc tgcccgatcc tcatcctcta caacaccgta catcactgtg tccataatag
     6601 gaggaaggaa tactcttgga tcaaactgat ccttcttttt ctccagaagt tcataaattc
     6661 ctcctactga gggactggtg cgaagctttg gccgggctgc aaacagctcc ttcatattgc
     6721 gcgtcacagc caaacggata tccgtatcca cattgatctt attaatgccg cagcggatcg
     6781 ctgcctttaa ctgctcaatg gaaataccgt gggtatctgc aagctcccct cccagagcgt
     6841 tgatttcatc tacaatgtat ccgggaacgg tagatgagcc atgggaaaca agagccccaa
     6901 aaatccccag atggttcata cactcacgga ttgccaccgg gatttcctta cggagcttta
     6961 catttttccc ctttgaggct ccatgcatgg ttccatagga aatcgccagt gcatctaccc
     7021 ctgttttctg gaaaaattcc actgcggaca atggattcgt ataggtggag gaagctgcaa
     7081 acacatgatc ctccacaccg gccagaactc ccagctctcc ttctacgctg acgcctctgg
     7141 catgagcgta ttttacgacc tctctggtca gctccacatt ttcatcaaag ggaagggagg
     7201 agccgtcgat catgacagag gtatagcctc cctctatggc tgccacgcag gagtcaaagt
     7261 ctctgccgtg atccagatgg agcaccacag gaatggctgc tgtctgagca tattttttta
     7321 ctgcgccggc aatatttctg gctccgatct tcttatcctc cagagtagcg tggataaaat
     7381 ccgtatttcc tcccataaag ccatttgcca gatccgcccc ctgcaggata gctgcagagc
     7441 ggaacatttc atgaacctct atggctgcct ttgcctgagc aaccgcattt acgttaaatg
     7501 ctccctggcc gaagccgtat ttcatggaag cctccaaaag aggccgcagg ggaattaaag
     7561 acatggatct ccctccttca tcatcaaatg gactgctccc agcttaaagg tctttggcag
     7621 agccaatata tttgggtttg gtcctacaaa ttttttcttt gcgtcctcca gagcctcctc
     7681 aatggtggct ctggtcttaa gacccattcc tctggcatag ccgggttcct gggcaccgca
     7741 tacataaatt gccgacgtat tcatttctgc aatatgggcc gaactcatca tggaaaagcc
     7801 gtggaacgga tggaaggcat tgcagtagcg gtacttgcgg atatattcct cattggtggc
     7861 aaaatattcg ccgtagcggt ttaaatccgg caggatattc atgtaatcct tctggaacat
     7921 atcgtaaagc tctctcatat agggccatct ctcatcatgg aaatagccgt tgcagatgga
     7981 agcgcagata atcacgcagt gatcgctcat gacccgttta tggcgcacta cctgtgcgga
     8041 aatggcctgc atcatcataa caggattggt tcccatgccg tcgccgtaat ggaacttctg
     8101 tggaaggccg aaaatcatta catcatactt cttttcagcc caggggatat aggtccgctt
     8161 gtcggccgta atccaggagt gtggctgcat cacctttgca tagccgctgt tgatctcaat
     8221 ctgtctggaa tacgtatcca aaaccgcgtc gcagcagaaa aacttctttc ccatgcattt
     8281 ttccatgtgc tggccgatct catcaaactt ggtccgcata agggagtggc cgtttaccgg
     8341 ggtaaaatcc ttccggtgca taacctgagg cacatgatgg gatgcaatgg aacgccagtg
     8401 ggtaattcct gtagaacagt gcttatatcc tccggaatag ccgccgtaag gatttccctg
     8461 ggtatggccg ataaggatcg ccacatcact gtcatacaca tacttattca taagaacagg
     8521 gtctccccgg tctgtcgttc ccagatcaac catgtgttca taatcctcgc tgtcatggct
     8581 gatgatctgg tgggtatacc agaattcgtg gaacaactcc tcccccagga tatcgcggat
     8641 ctccttttcc gtacttctgg gatgaaggcc gttggagcag atcagcagga tatccttttt
     8701 ttctaccccc acagagtaca gttcttgaag gatcagctta atggaaatct tccggtgagc
     8761 cgtgggctga agccctccct ttacaatatc cgggaaaata atgaccacct tggaaccctt
     8821 gtgggctaac tctgttaaag gcggcattcc catagggttg cggatggatt ttaaggtttc
     8881 ctcttctaat ttatcttccg gaatacaggg cggatccggc accgtctccc ctgggataaa
     8941 aatgtctgta gaatccggca gaacggcatc taccatgccc tgcccgtatt caaatgatac
     9001 tttcatataa aaactctcct tcccaaatgc tttaagcttt ttctgctgtc aggtacagct
     9061 ttacgatttc cagatattcc tccggataaa atccgatctc acctgaaaaa cgggtcagcg
     9121 caatcagtcc gccatccata tccggaacag aggcaagcat agcggcattg tcctgcttta
     9181 atccgcctcc gtaaaccaca tccatgtccc cggtaatctc ttttacaaat gctccgatct
     9241 tctggatata ctccttatcc ggcgggattt ttcccggccc gatagcccat acaggttcat
     9301 atgcaatggt tacgcagctc ttgtccacat cctttaatcc ctcctccagc tgcgtcctta
     9361 aaacctcctg ccacctgggc tgctcctctg aagtttctcc gatgcagtaa agcaccttca
     9421 gaccagcccg tacagcctct ctgatttcct gatttaatat tccgtttaca gctgccgggg
     9481 ctcctcctcc cgctgcaagg atccctgcct tatcctttcg ttcctcacag tggccgatga
     9541 gcacataact gcatcccaaa gctctggctg cattagctgt g
//
LOCUS       DBMDUH010000104         9577 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_3388_length_9577_cov_5.149008, whole genome shotgun sequence.
ACCESSION   DBMDUH010000104 DBMDUH010000000
VERSION     DBMDUH010000104.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 9577)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9577)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9577
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3388_length_9577_cov_5.149008"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2870)
                     /locus_tag="ACZPCG_09250"
     CDS             complement(<1..2870)
                     /locus_tag="ACZPCG_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HIR1285277.1"
                     /translation="MAKNFLEVFPSLNIAEPLRELLALTEVEKITSTRDRSSIRVYVK
                     SPRLIHKQNIYDLEKGIKDQLFPDKQVTVKIQERYRLSGQYTPEKLLKIYKDSLLLEL
                     KHYSIIEYSMFRKSQIRFEQEDLMVLTVEDTMVNRDRTGELKRVLEKFFAERCGLPVE
                     VRFAYIPPSGGERRKQLERKIEKEAQAIYWRNHREELEAMGGFSAEGLEGKELLKQTA
                     GPQDALMEAPKDGAPWDMLMAQAASGEVGSGEEASLQAVPAGNAGSGSTGTGNNSPGK
                     EKRPQLNKGRKGWGRDNGFGEDKKFSVKRSNNPDVLYGRDFEDEFLEIETIDGEIGEV
                     TLRGQVLTCESRELRSGKFILTFDITDFTDTITAKMFIRPEIFDEVKNVIQKGMFLKI
                     KGVTTIDKFDGELTLGSIVGIKKGEDFTSKRMDTSIEKRVELHCHTKMSDMDGVSEVK
                     DIIKRAKKWGMTSIAVTDHGCVQAFPDANHALDKGDPFKVIYGVEGYLVDDTKQLVEN
                     SKGQSFKDSYVVFDIETTGFSPLKNKIIEIGAVKVVDGAITERFSTFVNPDVPIPFDI
                     EKLTGINDAMVLPYPPIDVILPQFLEFVGDSALVAHNAGFDVGFISHYAEEQGLPFQP
                     TVLDTVSLARLLLPNLNRFKLDTVAKALNISLENHHRAVDDAEATAEIFAAFVKMLRD
                     RDIENLDQLNRLSAMDAHTIMKLPTNHVIILAKNDIGRINLYRLVSWSHLDYFSRRPR
                     IPKSVLNAHREGLIIGSACEAGELYQALLRGAPDAEIARLVNFYDYLEIQPLGNNAFM
                     LRDEKSTVKTRDDLIEINKRIVDLGAQFGKLVCATCDVHFLDPQDEVYRRIIMAGQGF
                     KDSDDQAPLYLRTTEEMLEEFAYLGPDKAYEVVVTNTRKISQMCEKIAPVRPDKCPPV
                     IPDSDKTLTKICYDRAHEMYGDDLPQIVVDRLERELHSIITNG"
     gene            complement(2875..3948)
                     /gene="ispG"
                     /locus_tag="ACZPCG_09255"
                     /gene_synonym="gcpE"
     CDS             complement(2875..3948)
                     /gene="ispG"
                     /locus_tag="ACZPCG_09255"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HIR1285278.1"
                     /translation="MTRNDTRVVRIGDRVIGGGNPVLIQSMCNTKTEDVAATVAQILK
                     LEEAGCEIIRVAVPTMEAAEALKQIKKEIHIPLVADIHFDYRLAVAAIENGVDKIRIN
                     PGNIGSRERVKAVVDAAKARRIPIRVGVNSGSLEKDLIAKYGGVTAQGIVESALDKVK
                     MIEDMDYDHLVISIKSSDVMMCVKAHEEIAKKTDYPLHVGITESGTLMSGNIKSAVGL
                     GIILYQGIGDTIRVSLTGDPVEEIKSARRILKTLGLRKGGVEVVSCPTCGRTRIDLIG
                     LANRVETMVTGLEGLDGVKVAVMGCVVNGPGEAKEADIGIAGGIGEGLLIKKGEIVRK
                     VPEEQLLNVLKEELTAMAVERKK"
     gene            complement(3966..5015)
                     /gene="rseP"
                     /locus_tag="ACZPCG_09260"
     CDS             complement(3966..5015)
                     /gene="rseP"
                     /locus_tag="ACZPCG_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HIR1285279.1"
                     /translation="MNIILSILILGAIILIHELGHFLMAKVCGIGVVEFSLGMGPRLF
                     SVEMGETRYSIKAFPFGGSCAMLGEDEEADEESAFGNKSVWARIAVVAGGPVFNFILA
                     FFLAMILVGVSGGYTVPEVLGVEEGFPAKEAGLLPGDVITHVNGRSIHSYRDFTTYVF
                     THPGKELKLTWKREGEDGKKEKLSASVTPVLTENGQQLIGVQFQPGIEPADTLLELMR
                     QSVYEVQFWIRYVFDTFYMMFHGAVSVDDISGPVGIVSTIDQTVDEASQAGAGAVAMT
                     LVNFGVLLSANLGVMNLIPFPALDGGRLFFLFLEALRGKPVDQEKEAMIHTAGMALLL
                     AFMVFILFNDVRKLF"
     gene            complement(5020..6180)
                     /locus_tag="ACZPCG_09265"
     CDS             complement(5020..6180)
                     /locus_tag="ACZPCG_09265"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HIR1285280.1"
                     /translation="MIKKISVLGSTGSIGTQTLEIARYNQDIGITALAAGSNVELLEK
                     QVREFHPKIACLWKEEAARELKRRIRDLDVKVVSGMEGLMEAACEPEAQIVVTAVVGM
                     IGIRPTIAAMEAGKDIALANKETLVTAGHIIMPLAKEKGVKILPVDSEHSAIFQCLQG
                     AGENRIHKILLTASGGPFRGRSREQLAGVQPEDALKHPNWSMGRKITIDSSTMVNKGL
                     EVMEAHWLFDVDMDQVQVVIQPASVIHSMIEYEDGAVIAQLGTPDMKLPIQYALYYPE
                     RRFLPGDRLDFTKLKEITFDEPDMETFRGLKLAYEAGKAGGSLPTVFNAANELAVSLF
                     LEKKIPYLAITDLIQGAMENHKRKAAPNVDQILAAEQEAYDFIRETLRKERI"
     gene            complement(6231..7031)
                     /locus_tag="ACZPCG_09270"
     CDS             complement(6231..7031)
                     /locus_tag="ACZPCG_09270"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009298142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HIR1285281.1"
                     /translation="MFTTRLISGIILVLLAVLFVGKGGLILFGVTALISLVGLFELYR
                     VLGIENRSVGAVGYITALSYYGILWWEGHEYLNLMIILCLMVLMGLYVFTFPKYKTEE
                     ITGAFFGVCYVPVMLSFLYQTREMQDGVYLVWLIFLSSWGCDTCAYCSGMLLGKHKLA
                     PVLSPKKSIEGAIGGTLGSAILGLIYAWIFGSRMHGIEEPVIACTAACAIAAVISQIG
                     DLAASAIKRNHKVKDYGHIIPGHGGILDRFDSMIFTAPAIYFVLTFLG"
     gene            complement(7050..7769)
                     /locus_tag="ACZPCG_09275"
     CDS             complement(7050..7769)
                     /locus_tag="ACZPCG_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HIR1285282.1"
                     /translation="MALDTNAVIPAHVALILDGNGRWAKKRGLPRTMGHKEGCVTVEK
                     TVEIAARMGIRYLTVYGFSTENWKRSEDEVGALMQLFRYYMVRLLKIASANNVRVKMI
                     GDRSRFAKDIIAGINRLENETKDNTGLTFVIAVNYGGRDEIRRAAAKLAADAARGALD
                     PDAVTEDVFASYLDTAGIPDPDLLIRTSGELRLSNYLLWQLAYTELYVTDCLWPDFSQ
                     EELEKAILQYNKRDRRFGGVK"
     gene            complement(7866..8417)
                     /gene="frr"
                     /locus_tag="ACZPCG_09280"
     CDS             complement(7866..8417)
                     /gene="frr"
                     /locus_tag="ACZPCG_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HIR1285283.1"
                     /translation="MEDLKFYEDKMNKTLDVLVSDYAAIRAGRANPHVLDKIRVDYYG
                     TPTPIQQVGNVSVPEARMIVIQPWEKSLLKAIEKAILTSELGINPTNDGNCIRLVFPE
                     MTEDRRKEVAKDVKKKGDGAKVAIRNIRRDANDAFKKAEKAGDMSEDELAETTEKMQK
                     LTDKMVERVDKLVEEKTKEIMTV"
     gene            complement(8495..9196)
                     /gene="pyrH"
                     /locus_tag="ACZPCG_09285"
     CDS             complement(8495..9196)
                     /gene="pyrH"
                     /locus_tag="ACZPCG_09285"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HIR1285284.1"
                     /translation="MKMNRVFLKLSGEALAGPKKTGFDEDTVKEVARQVKLSVDAGVQ
                     VGIVIGGGNFWRGRTSEAIDRTKADQIGMLATIMNCIYVSEIFRNAGMKTQILTPFEC
                     GSMTKLFSKDRANKCFEQGMVVFFAGGTGHPYFSTDTGIALRAIEMDADCILLAKAID
                     GVYDSDPKLNPEAKKYDTISIQEVIDKQLAVVDLTASIMCMEHKMPMAVFSLNEPDGI
                     ANAMQGRINGTIVTA"
     assembly_gap    9215..9224
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9382..>9577
                     /locus_tag="ACZPCG_09290"
     CDS             9382..>9577
                     /locus_tag="ACZPCG_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285285.1"
                     /translation="MEYIGTLQDFKYRTFLGRFISQIGICSCPAYEADMTSVLKNLDA
                     AVSAAEEYGPNQYAVFDSKLH"
BASE COUNT         2311 a         2687 c         1963 g         2606 t
ORIGIN      
        1 ccgtttgtaa taatagaatg aagttcccgc tccagacggt ctactacgat ctgaggcaga
       61 tcatccccat acatttcatg ggctctgtca tagcagatct ttgtcagcgt cttatcggaa
      121 tccgggatga ccggaggaca tttatccggc cgcaccggag cgatcttttc acacatctgt
      181 gagattttgc gtgtatttgt caccactacc tcataggcct tatccggccc cagataggca
      241 aattcttcca gcatttcctc tgtggtcctt aaatacagag gcgcctgatc gtctgagtcc
      301 ttaaagccct gccccgccat gatgatccgg cggtatacct catcctgagg gtccagaaaa
      361 tgcacatcgc aggtagcgca gaccagcttg ccaaactgtg cgcccaaatc tacgatccgt
      421 ttgttgatct cgatcagatc gtccctcgtc tttactgtgc ttttttcgtc ccggagcata
      481 aacgcattat ttccaagagg ctggatttcc agataatcat aaaaatttac tagcctggca
      541 atctctgcat ccggcgctcc tctcaaaaga gcctggtaca gctctcctgc ctcacaggca
      601 gagccgataa taagtccttc tctgtgtgcg tttaaaacgc ttttggggat tctgggacgt
      661 ctggaaaaat aatccagatg cgaccagctc accagacgat acaggttaat gcggccgata
      721 tcattttttg ccagaatgat cacgtggttg gtgggaagct tcataatggt atgggcatcc
      781 atggcgctca ggcggttgag ctgatccaga ttttcgatat cacgatcccg gagcatcttt
      841 acaaaggccg caaagatctc tgccgtggct tctgcatcat ccacggcccg gtgatgattt
      901 tccagtgaaa tgttcagagc ttttgccact gtatccagct taaaacggtt cagattcggc
      961 agaagcagtc tggctaagga taccgtatcc agaaccgttg gctgaaaggg aagtccctgt
     1021 tcctccgcat aatggctgat aaagcccacg tcaaagcctg cattgtgagc taccagggcg
     1081 gaatctccta caaattccag aaactggggt aggataacgt caatgggcgg ataggggagc
     1141 accattgcgt cattgatgcc tgtgagcttt tctatatcaa agggaatggg aacatccggg
     1201 tttacaaagg tggaaaagcg ctctgtgata gctccgtcca ctacctttac cgccccgatc
     1261 tcaatgatct tatttttcag cgggctgaag ccggtggttt ctatgtcaaa aaccacatag
     1321 gaatccttaa agctctgtcc ttttgaattt tccaccagct gctttgtatc gtctaccaga
     1381 tacccctcta ccccgtagat gaccttaaag ggatctccct tatccaacgc atggtttgca
     1441 tccggaaagg cctggacaca gccgtgatcc gtcaccgcga ttgaggtcat tccccatttc
     1501 ttagctctct taatgatatc ctttacctca gagacaccgt ccatatcgct catcttggta
     1561 tggcagtgaa gctccacacg cttttcaatg ctggtatcca tacgcttgct ggtaaagtcc
     1621 tcgccctttt tgattcccac gatcgaacct aaggtcagtt cgccgtcaaa tttatcaatg
     1681 gtggttacgc ctttgatctt aaggaacatt cccttctgta ttacattttt tacctcgtca
     1741 aaaatctccg gacggatgaa cattttagct gtaatggtat ctgtaaaatc cgtaatatca
     1801 aaggtcagga taaatttgcc gcttcgaagc tcccggcttt cgcaggtaag cacctgccct
     1861 cttaaggtaa cctctccaat ctcaccgtca atcgtctcaa tttccagaaa ttcatcttca
     1921 aaatcacggc catagagcac atcgggatta ttcgaacgct tgacagaaaa ctttttatcc
     1981 tctccaaagc cattatcccg gccccagccc tttctccctt tatttaactg gggacgcttt
     2041 tcctttccgg gagagttgtt tccggttccg gtacttccag acccggcatt tcctgctgga
     2101 acagcctgaa gggacgcttc ctctccgctt cccacctctc ctgaagccgc ctgtgccata
     2161 agcatatccc agggggcgcc gtccttagga gcttccatca gagcatcctg agggccggcc
     2221 gtctgcttta aaagctcttt tccctccaga ccctctgccg aaaagcctcc catggcttcc
     2281 agctcttccc tgtggttgcg ccagtagata gcctgtgcct ccttttcaat ctttcgctca
     2341 agctgttttc tgcgctctcc gccggaaggg ggaatatagg caaagcgcac ctctaccgga
     2401 aggccgcagc gctctgcaaa aaacttttcc agtacccgtt ttaactctcc ggtccgatcc
     2461 cggttcacca tggtatcctc tacggtcagt accatcagat cctcctgttc aaaccggatc
     2521 tgtgattttc tgaacatgga gtattcgatg atgctgtaat gcttcagttc caaaagcagg
     2581 ctgtccttat agatctttaa cagcttttcc ggcgtatact gaccggaaag acggtatctt
     2641 tcctggattt ttactgttac ctgcttgtca ggaaaaagct gatcctttat tcccttttcc
     2701 agatcgtaaa tattctgttt gtgtatgagc cgcgggcttt tcacataaac gcgaatagag
     2761 cttctatctc tggtagaggt aatcttttct acctctgtca gagctaaaag ctctctaagc
     2821 ggctctgcaa tattcagtga aggaaatacc tccagaaaat tcttcgccat aaaattactt
     2881 ctttctttcc actgccattg ctgttaattc ctctttcagc acatttaaca gctgttcctc
     2941 cggcaccttt cggacaattt cgcctttttt gatcaaaagc ccctctccga ttccgcctgc
     3001 aatcccgata tctgcttctt ttgcctcgcc cggtccgttt accacacagc ccataacagc
     3061 aacctttacg ccatccagtc cctcaagacc ggtgaccatg gtttctaccc ggttggccag
     3121 tccgatcaga tcaatgcgtg tgcggccgca ggtggggcag gaaacaacct ctactccgcc
     3181 ttttcttaag cccagagtct ttaaaatccg cctggcagac ttgatttcct ccaccggatc
     3241 gcctgtaaga gatacccgga tcgtgtctcc gattccctgg tacagaataa tccccagacc
     3301 taccgcggac ttaatatttc cggacataag agtaccggat tctgtgatgc ctacatgaag
     3361 cggataatcg gtctttttgg caatctcctc atgggctttc acgcacatca ttacgtctga
     3421 ggatttaatg ctgatcacca gatgatcgta atccatgtct tctatcattt tcaccttatc
     3481 cagcgcactt tctacaatcc cctgtgcagt cacacccccg tactttgcaa tcagatcctt
     3541 ttccagagaa ccgctgttta cgcctacacg gatgggaatc cggcgcgcct ttgcagcatc
     3601 taccacagcc ttcacgcgtt ccctgcttcc gatatttccg ggattgatac ggatcttatc
     3661 cactccgttt tctatggctg ccaccgcaag gcgataatca aaatggatat ctgccaccag
     3721 gggaatatgg atctcctttt ttatctgttt cagggcctct gccgcctcca tcgttggcac
     3781 agccacccgg atgatctcgc atccggcctc ctccagttta aggatctgag ccactgtagc
     3841 cgccacatcc tctgtttttg tattgcacat ggactggatc agtaccggat ttcctcctcc
     3901 gatcacccgg tctccgatcc tgaccacccg tgtatcgttt cttgtcatct taacgtttcc
     3961 tcctgttaaa acagctttct cacatcgtta aacagaataa ataccataaa cgccagtaaa
     4021 agcgccattc ccgctgtatg gatcatggct tccttttcct ggtcaacagg ctttcctcga
     4081 agagcctcca aaaacaggaa aaacagcctt ccgccatcca gggccggaaa agggatcagg
     4141 ttcatcacac ccagattggc gcttaaaaga acgccgaaat ttaccagagt catcgccaca
     4201 gccccggctc ccgcctgtga cgcctcatcc acggtctgat cgatggtgct tacgattcca
     4261 accgggccgc tgatatcatc aaccgatacg gctccatgaa acatcatata aaaggtatca
     4321 aatacatatc ggatccagaa ctgtacctca tataccgact ggcgcatcag ctccaaaagt
     4381 gtatctgccg gttcaatccc aggctgaaac tgtacgccaa tgagctgctg tccattttct
     4441 gtaagcacag gcgtcacaga agcgctcagt ttttcctttt ttccatcttc tccttccctt
     4501 ttccaggtga gcttaagctc ctttcccgga tgggtaaaca cataggttgt aaaatcccgg
     4561 taggaatgga tggaacggcc attgacatgg gtaatcacat ctcctggtaa aagaccggcc
     4621 tcctttgcag gaaagccctc ctctactccc aaaacctcag gaaccgtata gccgccggaa
     4681 acgcccacca agatcattgc cagaaaaaat gccagtataa aattaaatac agggcctccg
     4741 gccacaacgg caattctggc ccagaccgat ttatttccaa aggcgctctc ctcatccgct
     4801 tcctcatctt ctcccaacat ggcacaggaa ccgccaaaag gaaaggcctt gatggaatac
     4861 ctggtttccc ccatctccac actgaacaat ctgggcccca tacccagaga aaactccact
     4921 accccgattc cacaaacctt tgccatcaga aaatggccca gttcatggat cagaataatg
     4981 gcccccagga tcagtattga taaaatgata ttcatctggc taaatgcgtt cctttcttaa
     5041 tgtctctctg ataaagtcat atgcctcctg ctccgctgcc agaatctgat ccacattcgg
     5101 agccgctttt ctcttatggt tttccatcgc gccctggatc agatctgtga ttgctagata
     5161 ggggattttt ttctccagaa acaatgaaac cgccagttca ttggccgcgt taaacacggt
     5221 aggcagggag cctccggctt ttccggcctc gtaagccagc tttaagcccc ggaaggtttc
     5281 catatccggc tcatcaaacg taatctcttt taactttgta aaatccagac ggtctcccgg
     5341 aaggaaacgg cgctccggat agtaaagagc gtactgaatg ggcagcttca tatccggtgt
     5401 gccaagctgt gcgatcacgg ctccatcctc atattcgatc atggagtgga tcacgctggc
     5461 aggctggatc actacctgaa cctgatccat atccacatca aagagccagt gggcctccat
     5521 aacctccagt cccttattta ccatagtaga agaatcaatg gtaatcttgc gccccatgga
     5581 ccagttggga tgctttaagg catcctccgg ctgtacaccg gccagctgtt ctctggagcg
     5641 gcctctgaat gggcctcctg aagccgtcag aagaatcttg tggatccggt tctctcccgc
     5701 tccctgaagg cattggaaaa tagcagaatg ttccgaatct acaggaagaa tctttactcc
     5761 cttttccttt gccagaggca taatgatatg gcctgccgtc acaagggttt ccttatttgc
     5821 cagggcaata tcctttcccg cctccatggc cgctatggta gggcggatac cgatcatgcc
     5881 tactacagca gtcaccacaa tctgagcctc cggctcacag gctgcctcca tcaagccttc
     5941 catcccgctt accaccttca catccagatc cctgatccgg cgtttaagct ctctggccgc
     6001 ctcttctttc cagaggcagg cgattttggg atgaaattcc cgtacctgct tttccaaaag
     6061 ctccacattg gaacctgccg ccagcgcagt gatcccaatg tcctgattgt atcttgcaat
     6121 ttccagtgtc tgagtaccga tggaacccgt agaacccaga actgagatct tctttatcat
     6181 aacttatatt ctcctcattt tacgaaaata cacatttttc acaaaatcgt ttatcctaaa
     6241 aaggtaagaa caaaataaat cgccggagcg gtaaagatca tactgtcaaa ccggtcgaga
     6301 atccctccgt gccccggtat gatatgaccg tagtctttta ctttgtgatt acgtttaatg
     6361 gcagatgccg ccagatctcc gatctgggaa ataacagccg caatggcaca ggctgcggta
     6421 caagcaatca cgggttcttc aatcccgtgc attctggaac caaagatcca tgcataaatg
     6481 agacctaaaa tcgcagaacc aagagtaccg cctattgctc cctctatgga ttttttaggg
     6541 cttaacacag gagccagctt atgttttcca agaagcatcc cactgcagta agcgcaggta
     6601 tcacagcccc aggaacttaa aaagatcagc cacaccagat aaactccgtc ctgcatttcc
     6661 ctggtctgat acaaaaagga aagcatcacc ggaacatagc atacaccgaa aaaagcccct
     6721 gtaatctctt ctgtcttgta ctttggaaag gtaaatacat aaagacccat taacaccatc
     6781 agacagagga taatcataag gttcagatac tcatggcctt cccaccacaa aatcccatag
     6841 taagaaaggg ctgtaatata acccaccgca cctaccgaac gattttcaat ccccaaaaca
     6901 cgatacaact caaaaaggcc cacaagtgaa ataagggctg tgactccaaa caagatcagt
     6961 cctccctttc ccacaaaaag gactgccaga agcaccagta taattccgct tatcagtcgt
     7021 gtggtaaaca tggacttctg tctcctttcc tactttactc cgccgaaccg ccggtctctt
     7081 ttattatact gcaggattgc cttttccagc tcttcctggc tgaaatcagg ccacagacag
     7141 tctgttacat aaagctctgt ataagccagc tgccataaaa ggtaattaga caggcgcaat
     7201 tcaccgctgg tacggatcaa aagatccgga tccgggattc ccgccgtatc cagataagag
     7261 gcaaatacat cctctgttac agcatccgga tccagcgctc ctctggcggc gtcagctgcc
     7321 agttttgcag ctgccctgcg gatctcatcc ctgccgccgt aatttaccgc gatcacaaac
     7381 gtaaggcctg tattgtcctt tgtctcattt tccagacggt tgatacctgc aatgatatcc
     7441 tttgcaaagc gggaccggtc tccgatcatc ttcacccgga cattatttgc agaagcaatt
     7501 tttaacaggc gcaccatgta gtagcggaac agctgcatca gagctcctac ctcatcctcg
     7561 gagcgcttcc agttttccgt ggaaaatcca taaaccgtca gataacggat gcccattctg
     7621 gctgcaattt ccaccgtttt ctctactgta acgcagccct ccttatgtcc catggttctg
     7681 ggaagccctc tttttttagc ccaccgtccg tttccgtcta agatcagggc cacatgggcc
     7741 ggtattactg catttgtatc taaagccatt ctttctccgc ctttctctcc aggaatattt
     7801 ctgaatagat gaaaaggcag gagtcgctcc tgcctccaca tatgggaagt atccctttca
     7861 cggccttaaa cggtcatgat ttcctttgtc ttttcttcta ccagcttatc tacgcgctca
     7921 accatcttat cggtaagctt ctgcatcttc tctgtagtct ctgccagttc gtcctctgac
     7981 atatctcctg ctttttctgc ctttttaaat gcgtcatttg catcccggcg gatgttgcgg
     8041 atggcaacct tggctccatc tccctttttc tttacatcct tggcaacctc ttttctgcgg
     8101 tcctctgtca tctcagggaa gactaagcgg atgcagtttc cgtcattggt tgggttaatg
     8161 cccagctcag aggtaaggat tgccttttcg atggccttta acaggctctt ctcccatggc
     8221 tggattacga tcatgcgggc ctcgggaaca gatacattgc ccacctgctg aatcggggtc
     8281 ggagtgccgt aatagtcaac tctgatctta tcaagaacat ggggattggc acggcctgcg
     8341 cggatggcag cgtaatcaga taccagtacg tccagtgtct tgttcatctt atcctcataa
     8401 aactttaaat cttccatatg tcctcctgta atgcgggcct gtcggcctga ttttataaaa
     8461 ttcatacccg gcggcgcctg cgtcctgccg gaaactatgc ggtaacgatg gttccgttga
     8521 tccggccctg catagcgttt gcaatgccgt ctggttcatt taagctgaat acagccatgg
     8581 gcatcttatg ctccatgcac atgatggaag cagtcagatc taccactgcc agctgcttat
     8641 cgataacctc ctgaatggaa atggtatcat actttttcgc ttcaggattc agcttaggat
     8701 cgctgtcgta tacgccgtca attgcctttg ccagcagaat gcagtctgca tccatctcaa
     8761 tggcccgaag cgcaattccc gtatccgtag agaagtacgg atgaccggtt ccgcctgcaa
     8821 aaaataccac cattccctgt tcaaagcact tatttgcacg gtcttttgaa aacagctttg
     8881 tcatagagcc gcactcaaag ggggttaaaa tctgggtttt catccctgca ttgcggaaaa
     8941 tctcagatac ataaatgcag ttcataatcg tagccagcat gccgatctga tccgcttttg
     9001 tacggtcaat agcctcactg gtgcggcctc tccagaaatt cccgcctccg atcacgatac
     9061 ctacctggac acctgcatcc acagaaagct ttacctgtct ggcaacttct tttaccgtat
     9121 cctcatcaaa cccggtcttt ttcggtccgg caagtgcctc gccgcttaat tttaaaaata
     9181 ctcgattcat tttcatgggt gaatctctcc tttgnnnnnn nnnntttgtt cacttatcac
     9241 ctgacagccg aacatggcgg cagctgatac tctcattaca gtataccata ctccggtcca
     9301 tttgcaaaga aaaaggaaac aacttttata tttttcagca tttttgaatt aaatttgaga
     9361 ttttgcctgt attgtactat aatggaatat attggtacgt tgcaggattt taaatatcgc
     9421 acatttttag ggagattcat atcccaaatc ggcatctgct cctgtcctgc ctatgaagcg
     9481 gatatgacct cggtcctgaa aaatctcgac gctgccgtat ctgccgctga ggaatacggt
     9541 ccaaaccagt acgctgtctt tgactccaaa cttcacg
//
LOCUS       DBMDUH010000105         9456 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_3426_length_9456_cov_7.203207, whole genome shotgun sequence.
ACCESSION   DBMDUH010000105 DBMDUH010000000
VERSION     DBMDUH010000105.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 9456)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9456)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9456
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3426_length_9456_cov_7.203207"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="ACZPCG_09295"
     CDS             <1..426
                     /locus_tag="ACZPCG_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HIR1285286.1"
                     /translation="IKINIGAASRRPEVLTMEVRGRNLVTGLPKTIVVSSEETLEALR
                     EPALQIVDAVHNVLERTPPELAADIFDRGIVMTGGGSLLSGLDALIEEKTGITTVIAE
                     DPLTAVAIGTGKFIEFAHGMTSALEASMGIGGGKERDDY"
     gene            533..2821
                     /gene="recD2"
                     /locus_tag="ACZPCG_09300"
     CDS             533..2821
                     /gene="recD2"
                     /locus_tag="ACZPCG_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015574722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SF1B family DNA helicase RecD2"
                     /protein_id="HIR1285287.1"
                     /translation="MATITGFIEKIKFRNEENGYTIMTVIDQSDGDEVVMVGTVSYAA
                     EGDMIQATGRMTEHPVYGEQLQIESYELKNPEDAASMERYLGSGAIKGVGAALAARIV
                     RRFKADTFRIMEEEPERLSEIKGISEKMAMAIGEQVQEKRDMRQAMMFLQNYGITLNL
                     AAKIYQEYGPKLYSIIKENPYRLADDIPGVGFKMADEIARKVGIFTDSDYRIKAGILY
                     VLLQASANGHTYLPKSRLFEQASELLKVAPETMDKHLMDMQIDRKLVVKMPFCEEGQT
                     ADPLIYSSHYYYLELNTARMLHDLNIRGDIPETEIKTNLLKIQKQEKIHLDELQEQAV
                     YEAVNSGLLVITGGPGTGKTTTINAIIRYFEAEGMEILLGAPTGRAAKRMTEATGWEA
                     RTIHRMLELVPIGITGSGNNASSGNSTAGMRFDRNEENPLDADVIIIDEMSMVDISLM
                     HSLLRAVNVGTRLILVGDVDQLPSVGPGNVLKDIIGSGCFNVVKLTRIFRQAAQSDII
                     VNAHRINEGETVDLSKRSRDFLFIRRDNPDAVISAAITLIQKKLPGYVQAQENEIQVM
                     TPMRKGALGVERLNQILQNHLNPPSPEKKEKEAGGIIYRTGDKVMQIRNNYQLEWEIR
                     SRYGIPIDKGSGVFNGDMGVIREINEYAELLTVEFDEGKLVEYSFKQLEELELAYAIT
                     IHKSQGSEYPAVIIPMYSGPRMLMTRNLLYTAVTRARSCVCLVGRPDAFYTMASNAME
                     QERCSGLKVRIEEIYKEESCTQ"
     gene            2809..3543
                     /locus_tag="ACZPCG_09305"
     CDS             2809..3543
                     /locus_tag="ACZPCG_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003509664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HIR1285288.1"
                     /translation="MHPIMPLSRLYAGFLDLLYPRRCPVCSEIVMPKGNLICPDCMEK
                     LAFVRRPVCKCCGKEIISPEAEYCFDCTRHPHSFDSGMALINYNDTASRSMALIKYHG
                     RREYLDFYSEAMFRRFGPAIRRLSPDCIIPVPVHKSRLKKRGFNQAQELGIRLSAKTG
                     ISMRTEFLKRTKKTLPQKELDAGARLKNLEQAFVCGRLPSGIRTVLLVDDIYTTGSTM
                     EACSRALKNAGAERIIPAVICIGSGR"
     gene            3686..3850
                     /gene="rpmG"
                     /locus_tag="ACZPCG_09310"
     CDS             3686..3850
                     /gene="rpmG"
                     /locus_tag="ACZPCG_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HIR1285289.1"
                     /translation="MEVEVVRTKITLACTECKQRNYNMTKDKKTHPDRMETKKYCRFC
                     KRHTLHKETK"
     gene            3877..4131
                     /gene="secE"
                     /locus_tag="ACZPCG_09315"
     CDS             3877..4131
                     /gene="secE"
                     /locus_tag="ACZPCG_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008726234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HIR1285290.1"
                     /translation="MEENTVNTAVETTEKAARADKKTAKHEKKPSFFKGLKKEYKKIV
                     FADKETVAKQTVAVVIMSIFIGVLIGVLDMVMKFGLSFIL"
     gene            4153..4668
                     /gene="nusG"
                     /locus_tag="ACZPCG_09320"
     CDS             4153..4668
                     /gene="nusG"
                     /locus_tag="ACZPCG_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HIR1285291.1"
                     /translation="MSEAHWYVVHTYSGYENKVKVDIEKTIENRNLQDQILEVSVPML
                     PVVELKNGVEKKADKKMFPGYVLINMVMNDDTWYVVRNTRGVTGFVGPGSKPVPLTEE
                     EMASLGFVKTDVLVDFEVGDMITVISGAWKDTVGAIKAVNDSKQTITINVEMFGRETP
                     VELGFAEVKKM"
     gene            4770..5195
                     /gene="rplK"
                     /locus_tag="ACZPCG_09325"
     CDS             4770..5195
                     /gene="rplK"
                     /locus_tag="ACZPCG_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HIR1285292.1"
                     /translation="MAKKVTGYIKLQIPAGKATPAPPVGPALGQHGVNIVQFTKEFNA
                     RTADQGDMIIPVVITVYADRSFSFITKTPPAAVLIKKACSIKSGSGVPNKTKVAVLKK
                     ADLQKIAETKMPDLNAASLEAAMSMVAGTARSMGVTIEE"
     gene            5273..5968
                     /gene="rplA"
                     /locus_tag="ACZPCG_09330"
     CDS             5273..5968
                     /gene="rplA"
                     /locus_tag="ACZPCG_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HIR1285293.1"
                     /translation="MKSGKRYAEAMKNVDRAALYDVADAISIVKKNASAKFDETVELH
                     IRTGCDGRHADQQIRGAVVLPHGTGKTVRILVFAKGPKADEAQAAGADFVGAEELIPK
                     IQNEGWLDFDVVVATPDMMGVVGRLGRVLGPKGLMPNPKAGTVTMDVTKAINDIKAGK
                     IEYRLDKTNIIHVPVGKASFTEEQLADNFQTLMDAILKAKPSTVKGAYLKSVALTSTM
                     GPGVKLNVAKLSN"
     misc_feature    6077..6213
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            6263..6763
                     /gene="rplJ"
                     /locus_tag="ACZPCG_09335"
     CDS             6263..6763
                     /gene="rplJ"
                     /locus_tag="ACZPCG_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008726242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HIR1285294.1"
                     /translation="MAKVELKQPIVDEIKAMLDGACGAVVVDYRGLTVEQDTRLRKQL
                     REAGVSYKVYKNTLIKRAAEGTEFAALDPHLEGPTALAVSKDDATAPARILAEFAKTA
                     PNLELKGSVVEGTYYDQAGTQVIATIPSREVLLGKLLGSIQSPITNLARVLNQIAEKN
                     GGAAEA"
     gene            6870..7244
                     /gene="rplL"
                     /locus_tag="ACZPCG_09340"
     CDS             6870..7244
                     /gene="rplL"
                     /locus_tag="ACZPCG_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HIR1285295.1"
                     /translation="MAKLTTAEFIEAIKELSVLELNELVKACEEEFGVSAAAGVVVAA
                     AGAGAAAAEEKTEFDVELTEVGPNKVKVIKVVREATGLGLKEAKDVVDGAPKVLKQGA
                     SKEEAEDIKTKLEAEGAKVTLK"
     gene            complement(7347..7901)
                     /locus_tag="ACZPCG_09345"
     CDS             complement(7347..7901)
                     /locus_tag="ACZPCG_09345"
                     /inference="COORDINATES: protein motif:HMM:NF040203.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285296.1"
                     /translation="MKRFTKTMAALGLAAVMGTGSAFISLADVTTSVNPVATSRKSGW
                     VDVQNHWYYFDANGNPVKNQWIQDGNNRYWMQEDGEMSKQKWVYTEGQWYWVNAQGAR
                     ASNIWVEDGGSWYYMGGDGRMMTNTWLDYAGKWYYLTETGAAARGWKELGEKWYFFND
                     SDCSMAKDTMVGQYRVDVNGVYIA"
     gene            complement(8084..9079)
                     /locus_tag="ACZPCG_09350"
     CDS             complement(8084..9079)
                     /locus_tag="ACZPCG_09350"
                     /inference="COORDINATES: protein motif:HMM:NF024194.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HIR1285297.1"
                     /translation="MNLQNTYTANTILSAGSDAQKTDHVCKAVLKSRWVLANILKGVI
                     PEYADTPVHEIAETYIEPDQIELYLPVNGFPEQIQGLDKEDTAYLQTTVYYDVRLRAL
                     LPNRTKTQVYLYIDVEAQNAARESRLGYPLEKRAIYYMSRMISNQLQTVSRDTNYNVI
                     QKVYSIWICMGDDIPKNMQQSITGFTIAKKDIYRKIDIPKKNYDLMSAVFIRLGHGET
                     EEHLLGMLQTIFTDNAPPEERLKKLETEYGIPRTKEFNEEVSKMCTYSQFMLDRGEKE
                     GLEKGLKTGLETGKYKTYYDLISDGTLTLKQAAEKCSLSPDEFQKKLIELKITEA"
BASE COUNT         2671 a         2172 c         2283 g         2330 t
ORIGIN      
        1 atcaagatca atattggtgc ggcctcccgc cgtccggagg ttctgaccat ggaggtcaga
       61 ggccggaacc tggtgacagg ccttccaaaa accattgtag tttcctctga ggagacactg
      121 gaagcgctca gagagccggc tcttcagatt gtagacgcgg tacacaatgt attagagcgt
      181 actccgccgg agctggcagc ggatattttc gaccgcggta ttgttatgac aggcggcggc
      241 tcccttttaa gcggtctgga tgcgctgatt gaagaaaaga caggcattac cactgtgatc
      301 gctgaggatc ctttgacagc cgtagccatc ggaaccggta aattcatcga atttgctcat
      361 ggtatgactt ctgctctgga ggcttccatg ggaatcggcg gcggcaaaga gcgggacgat
      421 tattaaagct ttataaacca tagaagaacc cggatttcag ccgggttctt tttatcaccg
      481 gtccccatcc gagggaagaa tccatacaga agcaaaaggc ggtatcctta ctatggcaac
      541 catcacagga tttattgaaa aaataaaatt tcggaatgaa gaaaacggtt acaccattat
      601 gactgtcatc gatcagagcg acggagacga agttgtgatg gtgggaacgg tttcctatgc
      661 cgcagaaggc gatatgatcc aggccacagg ccgtatgaca gagcaccctg tttacggtga
      721 acagcttcag attgaaagtt atgagctgaa aaaccccgaa gatgccgctt ctatggagcg
      781 ctatctgggt tccggagcca tcaagggggt aggcgcagct ctggcagccc ggatcgtgcg
      841 ccgttttaag gcagatacct tccggatcat ggaggaagaa cccgaacgcc tgtcagagat
      901 taagggcatc agcgaaaaaa tggctatggc tatcggagaa caggttcagg aaaagcggga
      961 tatgcgtcag gccatgatgt ttcttcaaaa ctacggcatc accctgaatc tggctgctaa
     1021 aatttatcag gaatatggcc cgaagctgta cagcattatc aaggaaaatc cctatcgtct
     1081 ggcggacgat attccgggcg ttggctttaa aatggcagac gaaatagccc ggaaagtggg
     1141 aattttcaca gattccgatt accggataaa agcaggtatc ctgtatgtgc tcctgcaggc
     1201 gtctgcaaac ggtcatacct atctgccaaa gagccggctg tttgaacagg cgtcggagct
     1261 tttaaaggtg gcgcccgaaa ccatggacaa gcacctgatg gatatgcaga tcgaccggaa
     1321 actggtagta aaaatgccct tttgcgaaga aggccagacc gctgatccgc tgatctactc
     1381 ctcccattat tactatctgg aattaaatac tgcccgtatg cttcacgact taaacatccg
     1441 gggtgatatc ccggaaacag agattaaaac caaccttttg aaaatccaga agcaggaaaa
     1501 gatccacctg gacgagcttc aggaacaggc ggtttacgag gcagtgaaca gcgggctttt
     1561 agtcatcacc ggcggtcccg gtacgggaaa aaccaccacg atcaacgcga tcatccgtta
     1621 ttttgaggct gagggcatgg aaatccttct gggagcgcct accggccgcg cagccaagcg
     1681 catgaccgaa gctaccggct gggaggccag aaccattcac cgcatgctgg agctggttcc
     1741 catcgggata accggttctg gaaataacgc atcctccggc aattccacag cgggtatgcg
     1801 ttttgaccgg aatgaagaga atcccctgga tgcagacgtg atcattattg atgaaatgtc
     1861 tatggtggat atcagcctga tgcactctct tttgagagct gtcaacgtgg gaacccggct
     1921 gattctggta ggtgatgtgg atcagctccc cagcgtaggc cccggaaatg ttttaaaaga
     1981 tatcataggc tccggctgtt ttaatgtagt aaagctgacg cgtatcttcc gtcaggcagc
     2041 tcagagcgat atcattgtaa atgctcaccg catcaacgaa ggcgagacgg tagatctgtc
     2101 caagcgaagc cgggattttc ttttcatccg ccgcgacaat cctgatgctg tcataagtgc
     2161 cgccattact ctgattcaga aaaagcttcc cggctacgtc caggcacagg aaaatgaaat
     2221 tcaggtcatg acgcctatgc ggaaaggcgc tctgggggta gaacggctga accagatcct
     2281 gcagaaccat ttgaatcccc cttcgccgga gaaaaaggaa aaagaggccg gagggattat
     2341 ttaccgcacc ggagacaagg tcatgcagat ccgcaacaac tatcagctgg agtgggaaat
     2401 ccgcagccgc tatggtatcc ctattgacaa gggctccggc gttttcaacg gcgatatggg
     2461 tgtgatacgg gaaatcaacg agtatgcaga gctccttacc gtggaatttg acgaagggaa
     2521 actggtggaa tacagcttta agcagctgga ggagctggaa ttagcctacg ccattaccat
     2581 ccacaaatcc cagggaagcg agtatccggc tgtcattatc cccatgtatt ccggccctcg
     2641 gatgcttatg acccgaaatc tcctctatac cgccgtcacc agagcccgtt cctgtgtctg
     2701 tctggtagga cggcccgatg cattttacac catggcctca aatgcaatgg aacaggaacg
     2761 ctgttccggc ttaaaggtgc gaatcgaaga aatctataag gaggaatcat gcacccaata
     2821 atgcccctgt cccgtctgta tgccggcttt ctggatcttc tctatccccg ccggtgccct
     2881 gtgtgctctg aaattgtcat gccgaaagga aatctgatct gcccggactg tatggaaaag
     2941 ctggcctttg tccgccgtcc cgtgtgcaaa tgctgcggca aagagattat aagccctgag
     3001 gccgaatact gctttgactg taccagacat ccccattcct ttgattcagg gatggcgttg
     3061 atcaattaca atgatacagc cagccgttcc atggccctga tcaaatacca cggacgcagg
     3121 gaatatctgg atttttattc cgaggccatg ttccggcgct ttggcccggc gatccgccgc
     3181 ctttctcctg actgcattat ccctgtgccc gtccataaaa gccgcctgaa aaagcgggga
     3241 tttaatcagg ctcaggagct ggggatccgc ctttcagcaa agaccgggat ttccatgaga
     3301 actgaatttc tgaaacgtac aaaaaagact ctgccccaaa aggaactgga tgcgggagca
     3361 cgcctcaaaa atctggaaca ggcctttgta tgcggcaggc tcccgtccgg aatacgcacc
     3421 gtacttctgg tggatgatat ctacaccacc ggaagcacca tggaagcctg ctccagagcc
     3481 ttaaagaatg ccggggcaga gcggattatc cctgcggtca tctgtatcgg ctcaggccgt
     3541 taaaaactct tcataaaaaa gaccgtcctt cctcttgacg aacgccggat ttttgtgtta
     3601 tattatacgg gcgaatggaa gacaggtctt ttttttatgc tcaaaattaa gacctgaagc
     3661 aactcaatga tttacgataa attaaatgga ggtggaagtc gtgcgcacaa agatcacact
     3721 ggcatgtaca gaatgcaagc agcgtaacta caacatgacg aaggataaga agactcatcc
     3781 ggaccgcatg gagaccaaga agtactgcag attctgcaag agacatacgt tacacaagga
     3841 aacaaagtaa tcctgttcag taaaggacgt gaaattatgg aagaaaatac agtgaatact
     3901 gctgtagaaa ccaccgaaaa agcggccagg gctgacaaga aaacggctaa gcacgagaaa
     3961 aagccaagct ttttcaaggg cctgaaaaaa gagtacaaaa agatcgtctt tgctgataag
     4021 gaaaccgtag ccaagcagac agttgcagtg gtgatcatgt ccattttcat cggtgtactg
     4081 atcggcgtac tggacatggt tatgaaattc ggtttaagtt ttattcttta attcaaaggc
     4141 gagggtgatt gtatgtcaga agcacattgg tatgtagtcc atacctactc aggttatgag
     4201 aacaaagtca aagtcgatat cgagaagacc attgaaaaca gaaatcttca ggatcagatc
     4261 cttgaagtgt ctgtgcccat gctccctgta gtagagctga aaaacggcgt ggagaagaaa
     4321 gccgacaaaa agatgttccc gggttatgta ctgatcaaca tggtcatgaa tgatgacaca
     4381 tggtacgtag tacgcaatac ccgaggcgtt acaggctttg ttggtccggg ctccaaaccg
     4441 gttcctctga cagaagaaga gatggcaagt ctgggcttcg taaagacaga tgtcttagtc
     4501 gattttgaag tgggagatat gataaccgtt atctccggtg catggaagga tactgtaggc
     4561 gccattaagg ctgtcaatga cagtaaacag accatcacca tcaatgtaga aatgttcggc
     4621 cgcgagacac cggtcgagct gggatttgct gaagtcaaga agatgtaagc agatctgtgg
     4681 aacagacaaa aagatcagct tttgcgggtg cgaaggctgg tgggagggac attcccgaca
     4741 ataccacatg attttaggag gtgcctaaga tggcaaagaa agtaacagga tatatcaaat
     4801 tacagattcc agcaggcaag gctacaccag ctccgccagt tggtccggct cttggtcagc
     4861 acggtgtaaa catcgtacag ttcaccaagg agttcaatgc tagaacagca gatcagggcg
     4921 acatgatcat ccctgtagtt attaccgtat acgctgacag aagcttcagc ttcatcacaa
     4981 agactccgcc tgcagcagtt ctgatcaaga aggcctgcag cattaagagc ggttccggcg
     5041 ttcctaacaa gaccaaggtt gccgtattaa agaaggctga tctgcagaag atcgcagaga
     5101 caaagatgcc agacttaaac gcagcaagcc tggaagcagc tatgagcatg gtagccggta
     5161 ctgcaagaag tatgggcgtt accatcgagg agtaatagga atacccgttg tatttgcaac
     5221 agagaacgtg ggagggagat tccccgtcaa taccactagg aggttatttt aaatgaaatc
     5281 aggaaaaaga tatgcagagg ctatgaagaa cgtagaccgc gctgcacttt atgacgtagc
     5341 agacgctatc tctatcgtta agaagaacgc ttctgcaaaa tttgacgaga ctgtagagct
     5401 tcatatcaga acaggatgtg acggacgtca cgctgatcag cagatccgcg gcgctgttgt
     5461 acttcctcac ggaaccggta agaccgttcg tattctggta ttcgcaaagg gaccaaaggc
     5521 tgacgaggca caggcagcag gagctgactt tgtaggagct gaggagctga tcccgaagat
     5581 ccagaacgaa ggatggctgg atttcgacgt tgtagtagca actcctgaca tgatgggcgt
     5641 tgttggtcgt ctgggccgtg tacttggacc caagggctta atgccaaacc caaaggctgg
     5701 taccgtaacc atggacgtaa ccaaggctat caacgatatc aaagctggta agatcgaata
     5761 cagacttgac aagacaaaca ttatccatgt gccagtagga aaagcttcct tcaccgagga
     5821 gcagttagcg gacaacttcc agacgcttat ggacgctatc ttaaaggcaa agccaagcac
     5881 tgtaaagggc gcatacttaa agagcgtagc tcttacctct actatgggtc ctggcgtaaa
     5941 gctgaacgta gcaaagcttt ccaactaatt tggagtccag aatatagaat cacagcggac
     6001 agcgcatttt tgcgggtccg ctgttttata ttttcttgaa aaataagttg acattttaaa
     6061 ataaatgtgc tatcttagta caagtgttga atgcccgaag acagcaggta ccgtaaggtg
     6121 taagtttaac gcctgccgag gaacatacaa aactctgatc gagattttgc gtcctctctg
     6181 tccatgcggc agggaggttt tttatatccg ttcccatgct atggttttca atgcacaaat
     6241 actataagga ggtaaaccat tcatggcaaa agttgaatta aagcagccaa tcgttgacga
     6301 gatcaaggct atgctggacg gcgcatgcgg cgcagttgtt gttgactacc gcggcctgac
     6361 agttgagcag gatacaagac tgcgtaagca gttaagagaa gctggcgtgt cttacaaggt
     6421 atacaagaat accctgatca agcgtgccgc agaaggaaca gagttcgcag ctctggatcc
     6481 acatctggaa ggacctactg ctcttgcagt atccaaggat gatgctactg cacctgcaag
     6541 aatccttgct gagttcgcaa agacagctcc aaatctggaa ctgaagggct ccgtagtaga
     6601 aggaacctac tacgatcagg ctggtactca ggtgatcgct acaatcccat ccagagaggt
     6661 acttcttggc aagctgcttg gaagcatcca gtccccaatc accaatctgg cacgcgttct
     6721 caaccagatc gcagaaaaga acggtggcgc agcagaagca taagcttcaa tgcaaaattt
     6781 ggccgcccgc agccgcttag accggcatgg gccgcataag atgaaagacc atattatcga
     6841 aagtaaataa taaacaggag gaaattaaaa tggcaaagtt aacaaccgct gagtttatcg
     6901 aagctatcaa ggaattatcc gtattagaac tgaacgagtt agtaaaggca tgtgaggaag
     6961 agtttggcgt atctgcagca gcaggcgttg tagttgcagc agcaggtgct ggcgcagcag
     7021 cagctgagga gaagactgag ttcgacgtag agctgactga ggttggtcct aacaaggtta
     7081 aggttatcaa ggttgttcgt gaagctaccg gtcttggcct gaaggaagct aaggacgtag
     7141 ttgacggcgc accaaaggta ttaaagcagg gcgcttccaa ggaagaggca gaggatatca
     7201 agacaaagtt agaggcagag ggagcaaagg ttactctgaa gtaatctggc ttttcgctga
     7261 taacgtcctg agatatcatt cctatatcaa cggatataat aagaaccgca gatctgtgaa
     7321 aatggatcct gcggttcttt tttatactat gcaatatata caccgtttac atctacacgg
     7381 tactggccga ccatggtatc ttttgccata ctgcagtcgg aatcattaaa gaaataccat
     7441 ttttctccca gttctttcca tcctctggca gcagcgccgg tttctgtgag ataataccat
     7501 tttcctgcat aatccaacca ggtgttggtc atcatccgtc cgtcgcctcc catataatac
     7561 caggagccgc cgtcctcaac ccagatattg ctggcccgag caccctgggc atttacccag
     7621 taccactggc cttcggtata gacccatttc tgctttgaca tttccccgtc ttcctgcatc
     7681 cagtagcggt tgtttccgtc ctgaatccac tggtttttca cgggatttcc gtttgcatca
     7741 aaataatacc agtgattctg tacgtccacc catcctgatt ttctggaagt agccaccgga
     7801 tttacagaag ttgttacatc tgccagactt ataaaagcag atccggttcc catcactgct
     7861 gccaacccca aagcagccat tgtttttgta aaacgtttca taactcttct cctttctgat
     7921 aatcgtctga aaactaacgt ttcataatga tatagtatat ccgtcgtcaa attctgtcaa
     7981 ttaaaaaatg cagtaaaaaa gcccggcaga cgcagcagaa aatacatcct gccgcatgcg
     8041 ccggacctgt tttaaaacct ctccaaatat acgtgtcaat gtatcatgct tctgtgattt
     8101 ttaactcaat cagcttcttt tgaaattcat ccggggatag actgcacttt tcagctgcct
     8161 gttttaaggt caaagtccca tcagaaatga gatcgtagta ggttttgtat ttaccggttt
     8221 caagacctgt ctttaatcct ttttctagcc cttctttctc tcccctgtcc aacatgaact
     8281 ggctgtacgt acacatcttt gacacctcct cattaaattc ctttgttctg ggaatgccgt
     8341 attcggtttc aagctttttc agccgctcct ccggcggtgc attatctgtg aaaatggtct
     8401 gcagcattcc aagcagatgt tcctctgttt cgccatgccc cagccgtatg aaaacagcgc
     8461 tcatcagatc atagtttttc ttagggatat ctattttcct ataaatatcc tttttggcta
     8521 tggtaaaccc tgtaatagac tgctgcatat ttttagggat atcatctccc atacagatcc
     8581 atatgctgta gaccttttga atcacattat aattcgtatc tcttgaaacc gtttgaagct
     8641 gatttgaaat catacgggac atataataaa tggccctttt ttcaagcgga tatccaagac
     8701 ggctttcacg cgcagcattc tgggcttcca catcaatata caaatacacc tgagttttcg
     8761 tacggtttgg cagcagtgcc cgcagccgga catcatagta taccgtggtt tgtaaataag
     8821 cagtatcttc cttgtcgagt ccctgaatct gttccgggaa accatttacc ggcagataaa
     8881 gttctatctg atctggttcg atataggttt cggcaatttc atggactggt gtatccgcat
     8941 attcaggaat aacacctttc agtatatttg ccaaaaccca tcgtgacttc aaaacggcct
     9001 tgcatacatg atctgttttc tgggcgtcag aacccgccga aagaattgta ttagccgtat
     9061 atgtgttttg taaattcatt ccgtcctttc ccacgcactc ctgttttgat tatagcatcc
     9121 ggctatttaa gacgcaagag caacggaaac agttttcttt tcattaaatt tacatccatg
     9181 tacacagata aatacaataa tacacttatt caatactttt gtaataatta cgtaagaaaa
     9241 tgcacaggta aaagttatgg ctttttttcg gaatttataa tatactatgt catgtgatgg
     9301 agctggttat tcatccggga aatatcaata ttcctaaaaa taagcagttt ttgcaaaaaa
     9361 tgcaaaaaaa gtattgataa ttttccttat cggaagtata atctactcgt aatgcaatta
     9421 aggaatttca aaaagtattt caaaaacaaa aaggag
//
LOCUS       DBMDUH010000106         9446 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_3430_length_9446_cov_5.529695, whole genome shotgun sequence.
ACCESSION   DBMDUH010000106 DBMDUH010000000
VERSION     DBMDUH010000106.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 9446)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9446)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9446
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3430_length_9446_cov_5.529695"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="ACZPCG_09355"
     CDS             <1..642
                     /locus_tag="ACZPCG_09355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HIR1285298.1"
                     /translation="YLNGAEDDSAAGNGAACGQGSLSFRAPETGGRDSYFYDPGNPSV
                     HIIDMSENELEVPEDYTVIERREDMLSYTTEPFKEPFTITGELAVKLYVSCDCPDTDF
                     MVRITDVDETGRSIKLADGVLCAKYRNGFEQAEYLKPDQIYPVTIRTTKISNTFLPGH
                     SLRLTVTSSGKNFIFPNSNTEDGFDSTRMEKAHITVHRGAQTPSAAVMMREKD"
     gene            739..1656
                     /gene="pyrF"
                     /locus_tag="ACZPCG_09360"
     CDS             739..1656
                     /gene="pyrF"
                     /locus_tag="ACZPCG_09360"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HIR1285299.1"
                     /translation="MIRQLIEKIQKTKAPICVGLDPMLSYVPEHIQAAAFEQYGETLE
                     GAAEAIWQFNKEIVDHTFDLIPAVKPQIAMYEQFGIEGLKVYKRTVDYCKEKGLVVIG
                     DAKRGDIGSTSAAYATGHIGSVQVGSKTYSGFDTDFLTVNPYLGTDGVKPFVDVCNSH
                     DRGLFVLVKTSNPSSGEFQDRLIDGRPLYEWVAEKVVEWGNASMDGDYSNVGAVVGAT
                     YPEMSRILRNLMPHTYFLVPGYGAQGGTAEDLKHCFNKDGLGAVVNSSRGIIAAYKQE
                     KYKKFGTEHFAEASRQAVMDMVADINSVL"
     gene            1702..2487
                     /locus_tag="ACZPCG_09365"
     CDS             1702..2487
                     /locus_tag="ACZPCG_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HIR1285300.1"
                     /translation="MAKLKMTASVVSQAQLAEGIYDLRLKAPEIASQAAAGQFVSVYS
                     KDGSRLLPRPISLCGIDKEAGQLRLVYRVAGAGTEEFSALKAGDTVDILGPLGNGFSL
                     LEGKKAFLIGGGIGIPPMLQLAKELCALNGSEMVQSVIGYRDSQLFLKDEFEAYGPVY
                     TATEDGSRGTKGNVLNAIRENGLTADVIYACGPTPMLRALKAYAAENNIQCWISLEEK
                     MACGVGACLACVCQSKEKDEHSQVHNKRICKDGPVFLADEIEL"
     gene            2502..3404
                     /locus_tag="ACZPCG_09370"
     CDS             2502..3404
                     /locus_tag="ACZPCG_09370"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HIR1285301.1"
                     /translation="MNMSVNIAGVEFKNPVMEASGTFGSGMEYSEFVDLNRLGAVVTK
                     GVANVPWPGNPTPRIAETYGGMINAIGLQNPGIDVFAKRDIPFLKKYDTKIIVNVCGR
                     STEDYIEVVERLGEEPVDMLEINISCPNVKEGGIAFGQDPKAVEAITKAVKAHAKQPI
                     IMKLSPNVTDITVMAKAAEAGGADAISLINTITGMKIDVERRTFAVANKTGGLSGPAI
                     KPVAVRMVYQAANAVKLPIIGMGGIANAADALEFILAGATAVAIGTANFHNPYATVET
                     VDGIRAYMERHGIEDIKELIGAVK"
     gene            3519..4976
                     /locus_tag="ACZPCG_09375"
     CDS             3519..4976
                     /locus_tag="ACZPCG_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006781380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HIR1285302.1"
                     /translation="MDNQRYNVLFIQTDQWSQNFMGYRGCSEIMTPTIDQLARDGVIY
                     TNCYSTCPVCIPARRSLMTGMSPRSHGDRVYSDRMKMPEAKTLAQAFSDAGYQTVAVG
                     KLHVYPQRNRIGFNEVILQEEGRYEFGVLDDYQIWLAENGAVGQEFSHAMGNNTYYTT
                     PWPLERRMHPTVWTTAQMVRQIQRRDPTRPSFFYLSYQFPHPPLVPLKEYLDMYKDED
                     ITLPPAGDWVDDSFIMKEMTESGRHYSDREIKLAKKAYFAQCTLIDHQIRLVLGALRE
                     NGILDNTLIVFASDHGEMLFQHKMAGKRSFYENSSHIPLLFSGKPVASLRGKKDTRLA
                     CLEDIMPTLLEFCKIPVPDTSEGENLFGERKRQVLFGEISEGPRATRMIHDGRFKLIY
                     YPWGNRIQLFDLEQDPMEMKDLSGRSEVRQIQEALEKELMQQLYGEDVNWISDGKLTG
                     VEAPKYREKADYGFSNQRGLHWPVVMPVSKDSAGS"
     gene            5032..6729
                     /locus_tag="ACZPCG_09380"
     CDS             5032..6729
                     /locus_tag="ACZPCG_09380"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006860556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HIR1285303.1"
                     /translation="MKFSEMPYERIDFDAAKEELSSIIKAFQEAGTPQEQMEVHRRYY
                     KLMGRIKTQATLAEIRHSIDTTDPFYEEEKTYYDQEMPAFSNQEVQYKKLLFASPYRK
                     ELELVIGGPAFKNIELAAKSVSEAVIPLMQEENALVTEYEKLLAGAKIDWKGKLLNLS
                     MMTPYLNHEDREIRKKAAAKVNAFYEGIADQLDELYDKLVKNRTAQAKKLGFETYTDL
                     GYCRMNRNSYGRKEVEQFRDQIKKDWVPFAEKMWENRRKRLGLDTLYHMDEGLSFPEG
                     SPKPTGTPEEILKSGQKMYEELSPETREFFDFMMEGDLFDVFARPHKQVGGYMTYLPD
                     YHAPFIFANFNGTAGDVDVVTHECGHAFQGYISGKDPVMEHSDITMETAETHSMSMEF
                     FTNPWMEQFFGSRAGDFLKSQLEDAVVFIPYGSMVDEFQHIVYDHPEMTSAERKDIWK
                     KLEKQYKPHLTYEEESSFYAEGGFWQRQHHIYSFPFYYIDYVIAQTNAFQYKIWMQKD
                     YRAAWKSYLAFCEASASDFFTCMLKDAGLDSPFEPGIIRRIAAELEEIYNQMETDYL"
     gene            6842..7183
                     /locus_tag="ACZPCG_09385"
     CDS             6842..7183
                     /locus_tag="ACZPCG_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006861397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HIR1285304.1"
                     /translation="MKERREVLDYGMTFQDVYIDTPFHDPNWVLLRYKKNRKAFAWTY
                     ERNGTMWVNVKVDPQWREFWRKAYPSIQPGYHQNKEHWNSIPLDGRIPDEIIKRMIGE
                     SYDLIGGGKKQ"
     gene            7307..9446
                     /gene="feoB"
                     /locus_tag="ACZPCG_09390"
                     /pseudo
     CDS             7307..9446
                     /gene="feoB"
                     /locus_tag="ACZPCG_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611265.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
BASE COUNT         2617 a         1922 c         2606 g         2301 t
ORIGIN      
        1 tacctgaacg gagcagagga cgattcagct gccggaaatg gagccgcctg cggtcaggga
       61 agcctgagct ttagggctcc tgaaacaggt ggaagggatt cctattttta tgatcccgga
      121 aatccgtccg tacatattat agatatgtct gaaaatgaac tggaggttcc ggaggactat
      181 acggtgatcg aacggcggga ggatatgctt tcctatacca cagagccctt taaggagccc
      241 tttacgatta cgggcgaact ggcagtgaag ctgtatgttt cctgtgactg tcctgataca
      301 gattttatgg tccgaattac agatgtagat gaaacagggc ggtccattaa gctggcggat
      361 ggtgttttat gtgcaaaata cagaaatggc tttgaacagg ctgagtatct taagccggat
      421 cagatttatc ctgttactat ccgcaccacc aaaatatcca atacgtttct tccaggccac
      481 agccttcggc tgacggttac atccagcggt aaaaacttca ttttccccaa cagcaacaca
      541 gaagatggct ttgacagcac tcgtatggaa aaagctcaca tcacagtaca ccggggagca
      601 cagacgccgt ccgcagcggt gatgatgaga gagaaggact gagggaaagg acagggcgtt
      661 tcggggaaaa tgggtactgt tcagacggtg aaaactatga tagaattata acatatcaca
      721 agataagagg aggatattat gatccgtcag ttaattgaaa aaatacaaaa aaccaaggcg
      781 ccgatctgcg taggcttaga tcccatgctt tcctacgttc cggagcatat ccaggcggct
      841 gcttttgagc agtacggcga gacattagag ggagcggcag aggcaatctg gcagtttaat
      901 aaggaaattg tagatcatac ctttgatctg atcccggctg ttaagcctca gattgccatg
      961 tatgagcagt ttggtattga gggcttaaag gtatataaga gaactgtaga ctactgtaag
     1021 gaaaaggggc tggttgtaat cggcgatgca aagagaggcg acatcggctc tacctccgca
     1081 gcctatgcca ccggtcatat cggaagcgta caggtaggct ctaaaaccta cagcggcttt
     1141 gacacagatt tccttacggt aaacccatat ctgggcacag acggtgtaaa accatttgta
     1201 gacgtatgca acagccatga cagaggcctt ttcgttctgg taaagacctc caatccttcc
     1261 agcggcgagt ttcaggaccg cttgatcgac ggacgtccgc tttacgagtg ggtggcagag
     1321 aaggtagtag agtggggcaa tgcttccatg gacggagatt acagcaatgt aggcgcagta
     1381 gtaggagcaa cctatccgga gatgagccgt attttaagaa atctcatgcc tcatacctat
     1441 ttcctggtac cgggctacgg cgctcaggga ggtactgctg aagacttaaa gcactgcttt
     1501 aataaagacg gtttaggagc cgttgtaaat tcctccagag gtattattgc ggcctacaag
     1561 caggaaaaat ataaaaagtt cggtacagag cattttgcag aggcatccag acaggctgtg
     1621 atggatatgg tggcagatat taacagcgta ctgtaagatg aattacggca tcaggacgga
     1681 aatggaaata ggagaatgtt catggcaaag ttaaagatga cagcctctgt ggtttcacag
     1741 gcacagctgg ctgaaggtat ttatgattta agactgaagg ccccggagat tgcgtcacag
     1801 gcagcggcag gacagtttgt atccgtctat tccaaagacg gctcccgtct gcttccaaga
     1861 cccatcagcc tctgcggaat cgacaaagag gccggacagc tgcgtctggt atatagagta
     1921 gcaggggcag gcacagaaga gttttctgca ttgaaggcag gggatacggt cgatatcctg
     1981 gggcctctgg gaaatggctt ttctcttctg gaagggaaaa aagccttcct gatcggcggc
     2041 ggtatcggta ttcctcctat gctgcagctg gcaaaggagc tgtgtgcgct gaatggttct
     2101 gaaatggttc agtctgtgat cggctatcgc gacagccagc tgtttttgaa ggatgaattt
     2161 gaagcttacg gtccggttta tactgctaca gaggatggaa gccggggaac aaaggggaat
     2221 gtgctgaatg caatccgcga aaatggactt actgcagatg tgatttatgc ctgcggtccg
     2281 acccctatgc ttcgcgcctt aaaggcatat gcggcagaga acaacattca gtgctggatt
     2341 tctctggagg agaagatggc ctgcggtgtg ggcgcctgtc ttgcctgtgt ctgccagtct
     2401 aaggaaaagg acgagcattc tcaggtgcac aataagcgta tctgcaagga cggccctgta
     2461 ttcctggcag atgagattga actgtaaagg aggacggccc tgtgaatatg agtgtaaata
     2521 tcgccggagt ggaatttaaa aatccggtga tggaagcctc cggaaccttt ggctccggta
     2581 tggaatacag tgaatttgta gatttaaacc gtctgggcgc tgttgttaca aaaggcgtgg
     2641 caaatgtacc ctggcccgga aatcccactc ccagaatcgc ggagacctac ggcggtatga
     2701 tcaatgccat tggtctgcag aatccgggaa ttgacgtatt tgcaaagcgt gatattccat
     2761 ttttgaagaa atacgataca aaaattattg tgaatgtctg cggccgttcc acagaggact
     2821 acattgaggt agtagagcgt ctgggagaag agccggtgga tatgctggag atcaacattt
     2881 cctgccccaa tgtaaaagag gggggaattg ctttcggtca ggacccaaag gcggttgagg
     2941 cgattacaaa ggcagtaaag gctcatgcaa agcagccgat tattatgaag ctcagtccca
     3001 atgtgacaga tattaccgtg atggcaaagg ctgctgaggc aggcggtgca gatgcgattt
     3061 ccctgatcaa taccataacg ggaatgaaaa ttgacgtgga gcgccgtacc tttgccgtag
     3121 ccaacaaaac aggcggactt tccggccctg ctatcaagcc ggtggcagtg cgtatggtgt
     3181 atcaggcagc taatgcggtt aagctcccca ttattggaat gggcggtatt gccaatgcag
     3241 cggatgcttt ggagtttatt ctggcaggtg ctacggctgt agccattgga acagccaatt
     3301 tccacaatcc atatgcaact gtggagaccg tagacggtat ccgggcatat atggaacgcc
     3361 acggaataga ggatataaaa gaactgatcg gcgctgtgaa ataagcagcc gaagcaggga
     3421 tagaaacgat gacaaaagcc cgcgggagtc agaatccggc gggctttcgc tgacagacgc
     3481 ttttacaggg gggaagcagc actcgaaagg agaagggcat ggacaatcag agatataacg
     3541 tactttttat acagacagat caatggtctc agaattttat ggggtataga gggtgcagtg
     3601 aaattatgac tcccaccatt gaccagctgg caagagacgg ggtgatttat acaaactgct
     3661 acagcacctg tccggtatgt ataccggcca gacggagtct gatgacaggt atgagcccca
     3721 gaagccatgg tgacagggta tacagcgacc ggatgaagat gccggaagcg aaaacactgg
     3781 cccaggcatt ttcagatgcc ggatatcaga ctgtggcagt gggaaagctt catgtgtatc
     3841 cccagagaaa ccgtattggt tttaatgagg tgattcttca ggaagaggga cgctatgaat
     3901 ttggcgttct ggacgattat cagatatggc tggcggaaaa cggagcggta gggcaggagt
     3961 tttctcatgc tatgggaaat aatacatatt atacgactcc atggcccctt gaaagacgga
     4021 tgcatcctac cgtgtggact acggcacaga tggtgcggca gattcaaagg agagatccta
     4081 cacgtccttc cttcttttat ctgtcctatc agtttccaca tccgccgttg gttccgctga
     4141 aggaatatct ggatatgtat aaagacgagg atattactct gccgccagca ggagactggg
     4201 ttgacgacag ctttatcatg aaggaaatga cagaatccgg caggcattac agcgacagag
     4261 agataaagct ggcaaagaaa gcctattttg cccagtgtac tctgattgat catcagattc
     4321 gtctggtact gggagcgctg agggaaaatg gaatactgga taatacactg attgtgtttg
     4381 ccagcgacca tggggaaatg ctgttccagc ataagatggc ggggaaacgc agtttttatg
     4441 aaaattcatc ccatataccg ctgctctttt caggaaagcc tgtagcttcc ctgaggggaa
     4501 agaaggatac gcgtctggcc tgtctggagg atattatgcc tacccttctg gaattctgca
     4561 aaattccggt gccggatacg tcagaagggg aaaatctgtt tggtgagaga aagcgtcagg
     4621 ttctgtttgg agaaataagc gaggggccaa gagctacgcg catgatccat gacggacgct
     4681 ttaaactcat ttattatcca tggggaaaca ggattcagct ctttgatctt gaacaggacc
     4741 caatggaaat gaaagatctg tcagggaggt ctgaggtaag gcagatacag gaagcgctgg
     4801 aaaaggaact gatgcagcag ctgtacggag aagatgtaaa ctggatttcg gacggaaaac
     4861 tgacaggcgt agaggcgccg aaatacagag aaaaggcaga ttatggtttt tccaatcaga
     4921 gggggcttca ctggccggtg gttatgcctg tttcaaagga ttcggctgga agctgacagg
     4981 aatgataaaa taaaaatgat ccatactagt cataaacagg aggaacccaa catgaaattc
     5041 agtgaaatgc cctatgaacg gattgatttt gatgccgcca aagaggagct ttcctctatt
     5101 ataaaggctt ttcaggaagc cggtacgcct caggaacaga tggaggtcca cagacgctac
     5161 tataagctga tgggccgcat caaaacccag gctactctgg cggaaatccg tcacagtatt
     5221 gataccacag atccctttta tgaggaagag aaaacgtatt atgaccagga aatgcccgca
     5281 ttttccaatc aggaggtgca gtataagaag ctcctctttg cttctccata ccgcaaggag
     5341 ctggagctgg tcatcggcgg gcctgcattt aaaaatattg agcttgcggc aaagtctgta
     5401 agcgaggcag tgatcccttt gatgcaggaa gaaaatgccc ttgtgacaga gtacgaaaaa
     5461 ctgcttgccg gcgcaaagat tgactggaag ggaaaactcc ttaatctttc catgatgacg
     5521 ccatatttaa accatgagga tcgggaaatc cggaaaaagg cggctgcaaa ggtgaatgcg
     5581 ttttatgagg ggattgcaga ccagctggat gaactgtatg ataagctggt aaaaaaccgt
     5641 acggcgcagg caaagaagct cggctttgag acctatacag atctgggcta ctgccgtatg
     5701 aaccgcaaca gctacggaag aaaagaggta gagcagttcc gggatcagat taaaaaggac
     5761 tgggtgccct ttgcggaaaa aatgtgggaa aaccgcagga agcgtctggg tcttgacacc
     5821 ttgtaccaca tggacgaggg cttaagcttc ccggagggaa gccccaaacc aacaggaact
     5881 ccggaggaga tattaaagag cgggcagaaa atgtatgagg aattatcccc tgaaaccaga
     5941 gaatttttcg attttatgat ggaaggggat ctgtttgatg tatttgcccg tccccataag
     6001 caggtgggag gctatatgac ctatctgcct gattatcatg ctccttttat tttcgccaac
     6061 tttaacggaa ccgccgggga tgtggatgta gtgacccatg agtgcggcca tgcattccag
     6121 ggatatattt ctggaaaaga tccggttatg gagcattccg atatcaccat ggaaactgcg
     6181 gagacccatt ctatgtctat ggagtttttt acgaatccat ggatggagca gttcttcggc
     6241 agcagggccg gagatttctt aaaatcccag ctggaggatg cggtagtatt tattccctat
     6301 ggcagtatgg tagatgaatt tcagcacatt gtatacgatc atccggaaat gacttcggca
     6361 gagcgaaagg atatctggaa aaaattagaa aagcagtaca aacctcacct gacatatgag
     6421 gaggaatctt ccttttatgc agaaggtggc ttctggcagc gccagcacca tatctacagc
     6481 ttcccatttt attatattga ttatgtaatt gctcagacga atgcctttca gtataagatc
     6541 tggatgcaaa aggactacag agcagcctgg aaaagctatc tggcgttttg cgaagccagt
     6601 gcttcagact tttttacctg tatgttaaag gatgcaggtc tggattcccc ctttgagccg
     6661 ggaatcatcc ggcggattgc ggcagagttg gaagaaattt ataatcagat ggaaacagat
     6721 tatttataag cggagtgatt ttggcagaag attctgtggc tagcggaatc ttctgctttg
     6781 ctgtatgctt ttggcagagt gtttgttttg cagaaatggg aaggagaaaa gaagatttca
     6841 catgaaagaa cgcagagagg tattagatta tggaatgacc tttcaggatg tgtatataga
     6901 tacgccgttt cacgatccga actgggtact tcttagatat aaaaagaatc gtaaagcctt
     6961 tgcctggacg tatgagagaa atgggactat gtgggtgaat gtaaaggttg atcctcaatg
     7021 gagagaattt tggagaaagg catacccttc catacagccg ggataccatc aaaataagga
     7081 gcactggaat tcaattcctt tggacgggag gattccggat gaaatcataa agaggatgat
     7141 aggggaaagc tatgatctga tcggtggggg aaagaagcag taaaatgatt cgatttatag
     7201 ttagcttaaa caaagcgtgc ttgacaaatg ataaaaatta tcataaacta acttcgcaag
     7261 aatacagatg aacgtcgttt cagagtatga gaaaggacaa agaaaaatga cactgaagca
     7321 tttggaaata ggaaagagtg ctgtcataaa ggcagttggc ggggaaggcg ctctgcgcca
     7381 gcattttttg gatatggggg tgataccggg agcagaggta acggctgtga agctggcgcc
     7441 catgggagat cccatgcagc tgcagatcca cggttatgag ctgactctgc gcctggatga
     7501 tgcggatcag atcgagattg aacccattga catccgtacc cgcagccatg agggggcaaa
     7561 aaatattaac aacagtgttc atcccggcct gggagaggat ggaaaatatc atgtgaaggg
     7621 agagggaaat ccccttcctg agggtgaaat tcttacatat gccctggtag gcaaccagaa
     7681 ctgcggtaaa accactctgt ttaaccagct taccggagcc aatcagcatg tggggaattt
     7741 ccccggcgtt acggtagacc gcaaggatgg ccccatcagg gggtatgcca atacgcggat
     7801 tacggatctt ccgggtattt attctatgtc tccctataca aatgaggaga tcgtatccag
     7861 aaactttgtg ttggaggaca ggccgaaggc aatcatcaat attgtagacg ctaccaatat
     7921 tgaacgcaac ctgtatctta ccatgcagct tctggaaatg gatgttccca tggtaattgc
     7981 cttaaatatg atggatgaga tcacaggaaa cggaggcacc attgatgtaa acgggattga
     8041 agccatgctg ggagttccgg tagtaccgat ttctgcggca aaaaatcagg gtgtggatga
     8101 gctggtaaag catgcaattc acattgccag atatcaggaa cgtccggggc gtcaggattt
     8161 ttgtgatgaa aatgaatttg gcggagccgt gcacagaggc atccatgcgg tctgccatct
     8221 cattgaggat catgcaaaga gcgcgggaat cccgctgcgg tttgcagcct ctaagctgat
     8281 cgaaggcgat acgctgattt taaagcagtt aaagctggat cagaatgaag aggaaacctt
     8341 agagcacatc attttacaga tggaaaagga acgggggctt gaccgcagcg cagccatggc
     8401 ggatatgcgt ttttccttta ttgaacggat ctgcgaaaaa accgtggtaa agccaagaga
     8461 gagcagggag catatcagaa gccggagaat tgatcaggtt ctgactggaa agtatacggc
     8521 gatcccctgt tttatcggaa ttatggctgc ggtcttttac ctgaccttta atgtgatcgg
     8581 agcatggctt caggggattc tggaaatagg catcacggct ctgacagagt gggtggacgg
     8641 agctcttaca gcagctcatg taaatacggt tcttcacggc cttgtgattg acggtatctt
     8701 ttccggagta ggttctgtgc tttccttcct tccgattatt gtaacgctgt tctttttcct
     8761 gtccatgatg gaagacagcg gatatattgc aagagttgcc ttctttatgg ataagctgct
     8821 gcgaaagatc ggcctttccg gccggagcat cgtgcctatg ctcatcggtt ttggctgtac
     8881 tgttcctgca gtgatggcga cccggacgct tccaagcgaa agggaccgta agatgaccat
     8941 tctcttaaca ccctttatga gctgtacggc aaagcttccg atctatgcct tttttgtcag
     9001 cgcctttttt ccgggacatg gcggccttat tatgacaggc ctttatattc tgggaattct
     9061 ggtgggtatt ttagcggcag tcttatttaa gagcacccta tttaaagggg aggcggttcc
     9121 ttttgtaatg gagcttccca actaccgtct gccgggggtt aaaaatgtaa gccagctttt
     9181 atgggaaaag gctaaggatt ttctgcagag ggcttttacc gtaattctgg tagctacggt
     9241 ggctgtgttt tttgcagagc tttgacttcc agtttaatct ggtagaggat tcatcgggaa
     9301 gtatcctggc tgtgatttcc ggctggctgg ctccgctgtt tgcgcctctg ggactggggg
     9361 actggcggat cattacctct cttgtcagcg gctttatggc aaaagagagc gtggtatcta
     9421 ctctggaagt cctctttgca ggcggg
//
LOCUS       DBMDUH010000107         9416 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_3438_length_9416_cov_4.550188, whole genome shotgun sequence.
ACCESSION   DBMDUH010000107 DBMDUH010000000
VERSION     DBMDUH010000107.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 9416)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9416)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9416
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3438_length_9416_cov_4.550188"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     regulatory      complement(186..296)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(495..2582)
                     /locus_tag="ACZPCG_09395"
     CDS             complement(495..2582)
                     /locus_tag="ACZPCG_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR1285305.1"
                     /translation="MKFTIKHEIPGRIRIHMEQKQMSYKEADTLLYYLDTLEYVTQAK
                     VYRRTADACVCFSGKRELLIQALRKFRYTSVKVPDEVLTNSGRQLNDIYQEKLIGKVL
                     FHYAGKIFLPYPVRKAVVLWKAVHYLKEGWKSLARRKLEVAVLDASAIGISVLRGDFN
                     TAASVMFLLGVGETLEEWTHKKSVGDLARSMSLNVSKVWLKSEEQEVLVDSRKIKEGD
                     NIVVHMGNVIPFDGVVCEGEGMVNQSSLTGEPLPVLKKAGGYVYAGSVLEEGELTFTV
                     KKAGGSSRYEKIVEMIEESEKLKSSLEGKAERLADRLVPVTFAGTALTWLFTRNITKA
                     LAVLMVDFSCALKLTMPIAVLSAIRQAGSYDITVKGGKFLEAVAEAETIVLDKTGTLT
                     KAMPTVKMIVPFCEAGENELLRIAACLEEHFPHSMAKAVVSAAKKAGLEHEEMHSKVE
                     YIVAHGISSSIEGKRVFIGSAHFIFDDEKCTVPKEYQKQFESLPEEYSHLYLAIDGVL
                     AAVICIEDPLREESASVVRALKGAGFQKVVMMTGDNERTAAFIARKAGVDEYYSEVLP
                     EDKAMFIEKEKAKGKKVLMVGDGINDSPALSAADAGIAVSDGSELAREIADITIPADN
                     LSQIVRLKYLSQALMRRINRNYKEIVGINTLLILLGVGGMIQPTTTAMFHNMSTIAIS
                     LQSMQNLSEIEEV"
     gene            complement(2583..2879)
                     /locus_tag="ACZPCG_09400"
     CDS             complement(2583..2879)
                     /locus_tag="ACZPCG_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016283282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6110 family protein"
                     /protein_id="HIR1285306.1"
                     /translation="MDSLKLKNLALFAGGVLFGTAGVKILSSKDVKKVYVEGTAAVLR
                     ARDCVMKTAETVQENAEDILAEAKQINERRAAEEAASVQEDTVEDHADPETEEA"
     gene            complement(3099..3362)
                     /locus_tag="ACZPCG_09405"
     CDS             complement(3099..3362)
                     /locus_tag="ACZPCG_09405"
                     /inference="COORDINATES: protein motif:HMM:NF012621.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HIR1285307.1"
                     /translation="MLWRFFANQDKKKKLKNIIGQKRVQIEGMTCEHCKNRVENALNR
                     LEGVSAKVNLKKKTAVVSMEKEVEDMQLQKAVEQAGYKVIQIR"
     gene            complement(3441..3878)
                     /locus_tag="ACZPCG_09410"
     CDS             complement(3441..3878)
                     /locus_tag="ACZPCG_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HIR1285308.1"
                     /translation="MEKETIFRKLRACGGRITAQRKILLDIILEEECVCCKEIYYKAI
                     KKDPAIGIATVYRMVRSLEDIGAIRRNKMYEIQYGKERIEENALADKILARHSFFLKI
                     LVEAGVDQQQAEKEASMLEYILSDESFDKLKVYMEKGTWENGK"
     gene            complement(4003..4179)
                     /locus_tag="ACZPCG_09415"
     CDS             complement(4003..4179)
                     /locus_tag="ACZPCG_09415"
                     /inference="COORDINATES: protein motif:HMM:NF024081.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HIR1285309.1"
                     /translation="MADLLVTGILLLIIGAAAVYIWKAKKRGVKCIGCPSAGKCSGKE
                     GHTVCSCGCHSAEK"
     gene            complement(4200..6197)
                     /gene="feoB"
                     /locus_tag="ACZPCG_09420"
     CDS             complement(4200..6197)
                     /gene="feoB"
                     /locus_tag="ACZPCG_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HIR1285310.1"
                     /translation="MRIALTGNPNSGKTTMYNALTGRNERVGNWAGVTVEKKESPIKK
                     GYYGGSEELIAVDLPGAYSMSPFTSEESITSSYVKNENPDAIINIVDATNLSRSLFFT
                     TQLLELGIPVVVALNKSDITEKKKTEIDVKKLADKLGCPVIQTVSITSGHEGLKEVVN
                     HAAALKGRVQKAPYVQADIDLQDKAQVEAADRKRFEFVNGIVKAVEKRKVLTKDKNKQ
                     DSIDAVLTNKFVGIPIFAVVMFGVFYISQSTVGTWIADWLVGCIEAFQGWVAGLVEGA
                     NPFLQALLVDGIIGGVGAVVGFLPLVMVMYFLIALLEDCGYMARATVVLDPIFKRVGL
                     SGKSVIPMVIGTGCAIPGIMACRTIRNERERRATAMLTPFMPCGAKLPVIALFAGAFF
                     ADASWVGPTMYLAGAVLIFLGALLVKKITGYKFRKTFFIIELPEYKIPSLKRATLSML
                     SRGKAYIVKAGTIILVCNTAVQIMQSFNWQFQVVEEGMEHTSILASIASPFAVLLIPL
                     GFGVWQLAAAAITGFIAKENVVGTLAVVYGLTNFIDTDELALVGGANEVAMVMGLTKV
                     AALAYLMFNLFTPPCFAALGAMNSEMQDKRWLWGGIALQLATGYTVAFFVYQVGTLIT
                     TGTLGNGFVPGLIAVAAFAGALLVLIQRNEKAFSAEYRLHA"
     gene            complement(6276..6488)
                     /locus_tag="ACZPCG_09425"
     CDS             complement(6276..6488)
                     /locus_tag="ACZPCG_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008689714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HIR1285311.1"
                     /translation="MNLSEAKEGKEYIVKDILTDDEELNEFLFSLGCYSGEPITVITH
                     RKGGCVVSIKDGRYNIDTDLAQAISI"
     gene            complement(6691..7782)
                     /locus_tag="ACZPCG_09430"
     CDS             complement(6691..7782)
                     /locus_tag="ACZPCG_09430"
                     /inference="COORDINATES: protein motif:HMM:NF015038.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HIR1285312.1"
                     /translation="MIRKQRVVIEMSVKVIEYTAKEYRSRITGARVHAPFPPGYTNEV
                     NYDGTVKALAFLLSNECNVSHGRIRTLLSELTDGKVELSDGMLNHLSEKFVLKSGTEK
                     KEIISRLMSSPVMNADFTNANVNGQSAQVLVLASPVNGAALYIGREKKGHEGVKGTPL
                     EDYTGTVVHDHDRTFYSYGTRHQECTQHDCRYLIGSKENEPDLEWNHKMHALFQEMLH
                     YRNGLCEEEGPDPVIVADFETRYDEILDKAKEEYEASPPSNYYREGYNLSVRLREYKE
                     SELLFLHDKRVPVNNSLCERLARVYKRKQKQAMVLRSQKNLGYICDGLSTVHLLRTDA
                     RNVYQEIAAIFERKSPQKPKKEVEAGALT"
     gene            complement(7779..8042)
                     /locus_tag="ACZPCG_09435"
     CDS             complement(7779..8042)
                     /locus_tag="ACZPCG_09435"
                     /inference="COORDINATES: protein motif:HMM:NF024242.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR1285313.1"
                     /translation="MCQPILTISMFAKTFHCNFSKYVNGVRLNYAEALLQDSKESIIN
                     IALDCGFESQRTFNRVFRKRYKMTPREFRNRAMKLRAGEDGYD"
     gene            complement(8064..8972)
                     /locus_tag="ACZPCG_09440"
     CDS             complement(8064..8972)
                     /locus_tag="ACZPCG_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HIR1285314.1"
                     /translation="MMFIAIKTEDGAIKGKLSFYCQMLGVSRQGFYKYLANKDRPWKY
                     QDLADAMIAIHTEDEYNDTYGRIRMYQALLLKKPEGLKIPSERTVYRVMDEIGLSHQP
                     KRKPNGITKADREARKSDDLLKRDFKSDKPLEKCVTDITEIKAKDGKLYVSAIFDCFD
                     SSVLGLAMETNMKATLCEHTLDNAYLSYPDLRGAIVHSDRGTQYTSETYRKALAKYGI
                     IQSMNSAGGRCHDNARCESMWARMKSELLYDRYNTETMTIEELKVLIWRYFISYWNNR
                     RICSANGGIPPIIKRQRYYQSLEQAA"
     gene            complement(8969..9310)
                     /locus_tag="ACZPCG_09445"
     CDS             complement(8969..9310)
                     /locus_tag="ACZPCG_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009215075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR1285315.1"
                     /translation="MSRTQRKYDHEYKVQAVKLAREIGGAKAAKELGIPEGTIHTWLK
                     AVRSGTLDIGDGAHTPESAMSLAEELAMLRKRVKDQDKEIRRLKEENEFLEEASAFFA
                     ASRRKSARTKE"
BASE COUNT         2402 a         2442 c         1609 g         2963 t
ORIGIN      
        1 tgctgtcgct gcctgctatc gtcaataccg ttttcatttg aatctccttt ctgtttacaa
       61 aaagggaacg atcaagactc acagcgataa aacatacttc ggctgccata aaaacgaatc
      121 tcgcaagcag gattctccgc ctgcggcggg tcgcatcagc gacataatcc caccccggca
      181 ggtgcactct ctctttaaaa gcttccctac gttggcatta tccaaatcag gttcacgggt
      241 ctaagcgctg tcagcttact ctcagcccgc ttgcgcgagc tccccttttt atattacttt
      301 ttattataat ccttctgtcc taaaaagcaa gagggataac cggccttaaa ccagttatcc
      361 ctctctcata tcacatcatt tcagcagtca gcggagagtt ttccaaatcc tcctaaacta
      421 ttttatccgc aacagcttcc cagaatctct gttaaaccat ttttactgcc ggtatgacag
      481 cggatcacat ttgtttaaac ttcttctatt tccgaaagat tttgcatact ttgcaaactg
      541 attgctatcg tagacatatt atggaacatt gcagtagtcg taggctgtat cattcctccc
      601 actcctaaaa gaatgagtaa tgtgttaata cccactattt ctttatagtt cctgtttatt
      661 cttctcatca atgcctgact gagatacttc aatcgcacaa tctgagataa attatcagcc
      721 ggaatcgtaa tgtctgcaat ttctctggca agttctgacc catcgctgac tgcaatccct
      781 gcgtccgccg ctgaaagtgc cggagaatcg ttgatcccgt cgcctaccat gagtaccttc
      841 tttcctttgg ctttttcctt ctcaataaac atggctttgt cttccggcaa tacctctgag
      901 tagtactcat caacacctgc ctttcgggca atgaacgctg ctgtacgctc attgtctccc
      961 gtcatcataa caaccttctg aaatcctgcc cctttcaatg ctctcactac gctcgcgctt
     1021 tcctcccgca acggatcttc aatacagatc actgctgcaa gaactccgtc tatcgccaga
     1081 tataaatgag agtattcttc cgggagactt tcaaactgtt tctgatattc cttcggaaca
     1141 gtacattttt catcatcgaa aataaaatgt gcactgccta taaagacacg ttttccctct
     1201 atactggaag aaataccatg agctacaata tactccactt tcgagtgcat ttcctcatgt
     1261 tcaagtcctg ctttttttgc cgcagatact actgccttcg ccatagagtg cggaaaatgc
     1321 tcttccagac aggccgctat acgaagcagt tcattttcac ctgcttcaca aaatggaacg
     1381 atcattttca cggtaggcat agcttttgta agcgtgcctg ttttgtccag tacgatcgtt
     1441 tccgcttctg caaccgcttc taaaaatttt ccgcccttta ccgtaatatc ataagagcct
     1501 gcctgacgaa ttgcagacaa taccgcaatc ggcattgtga gttttaacgc acaggaaaaa
     1561 tctaccataa gcacagctaa agccttcgtg atatttcgcg taaagagcca tgtaagagct
     1621 gttcctgcga aggtcaccgg aactaatcga tctgcaagtc gttctgcttt tccttccaac
     1681 gaagatttca gcttttcaga ctcctcgatc atctcaacaa tcttctcata ccgagaagaa
     1741 cccccggctt tttttacagt aaaagtaagt tccccttctt ccagaacgct tccggcatat
     1801 acatatcctc ctgccttttt caaaaccggg agcggttcac cggtcagaga agactgattt
     1861 accattcctt cgccttcgca taccaccccg tcaaaaggaa taacattccc catgtgaact
     1921 acgatattat ccccttcttt gatttttctg gaatcaacaa gtacttcctg ttcttcactt
     1981 ttcagccaaa ccttcgacac attcaaagac atactgcgtg ccagatctcc taccgatttc
     2041 ttatgtgtcc actcctccaa ggtctctcca actcccaaca ggaacataac ggaagcagcc
     2101 gtattaaaat cacctcgcaa aacagaaatg ccaatagctg atgcatccaa cactgcgact
     2161 tccagcttcc gccttgcaag actcttccag ccttctttta aataatgtac agccttccat
     2221 aaaaccactg cttttctgac cggatatggc agaaagattt taccggcata atgaaaaaga
     2281 actttaccaa tcagtttctc ctgataaata tcattcaact gtcttccaga atttgttaac
     2341 acttcatccg gaacttttac agacgtatat cggaattttc ttaaagcctg aatcaacagc
     2401 tctcgttttc ctgaaaagca aacgcaggca tcggccgtcc ttctataaac ctttgcctgc
     2461 gtgacatatt ccagcgtatc caaataatac agtaatgtat cggcctcttt ataagacatt
     2521 tgtttctgtt ccatatgaat acggattctt cccggaatct catgctttat agtgaatttc
     2581 aattacgctt cctctgtttc aggatcagca tgatcctcta ctgtatcttc ctgtacagag
     2641 gcagcttctt ccgcggcgcg cctctcattg atctgctttg cttctgccag gatatcttct
     2701 gcattttcct gaacggtctc agcggtcttc atcacacaat ctcttgctcg aagaactgct
     2761 gctgttcctt ccacatatac ttttttcaca tccttactgg aaaggatttt cacacctgcc
     2821 gttccaaata atactccgcc tgcaaacaat gcaaggttct ttaattttaa actatccata
     2881 aatatcctcc atttctgtct tgtgcgattt tatttgtgtt tttttccggt atagaataac
     2941 atgacgagac aaacaatcat tgcccatgcc cataacctat gctgtttcat tctgtcacct
     3001 cttctctggt ttgttattat attctctcat atatatacag tcaaagtatt tttcttttaa
     3061 tgaaatattt tctcaacaaa tggcgtgccg caatcatatc accgtatttg tattacctta
     3121 taccccgcct gctcaaccgc tttctggagt tgcatatctt ctacctcttt ttccatagat
     3181 acaactgccg tttttttctt cagattcact tttgcagata ccccttccaa acggttcaat
     3241 gcgttttcaa cccgattttt gcaatgttca caggtcatgc cttcgatctg taccctcttc
     3301 tgacctatga tatttttcaa ttttttcttt ttgtcctgat ttgcgaagaa ccgccacagc
     3361 atcctccctg ccccttaaag tgctttgtgc cggaacggat tcctgtaaat accagaatca
     3421 aaagtacagc aattataatc tcatttacca ttttcccacg ttcctttctc catatatact
     3481 ttcagtttat caaaactttc atccgacaag atatattcca gcatactggc ctctttttct
     3541 gcctgctgtt gatcaactcc cgcctctacc aggattttta gaaaaaaaga gtgtctggca
     3601 agtattttgt ccgccaatgc attttcttca atcctctcct ttccatactg gatctcatac
     3661 attttgtttc ttcggatcgc tcctatatct tccagcgacc tgaccattct atagactgtc
     3721 gctattccga tcgcagggtc tttttttatc gctttataat aaatttcttt acagcagaca
     3781 cattcttctt ccaaaataat atccagaaga attttccttt gtgccgtaat acggcctcca
     3841 cacgctctta acttacggaa aattgtttct ttttccattt gtgtcctgga aaaatccgga
     3901 ttttcatttt tctggcagct tttcacagac ctcactccat ttcctgtgtt ttatataaaa
     3961 ggcccacacc ttttccaaag tgcgggcttt ccctgaaatt tcctattttt ctgcactatg
     4021 gcatccgcag ctgcacactg tatgtccttc ttttcccgaa cattttcctg cagatgggca
     4081 gcctatacat ttaacgcctc tcttctttgc tttccatata taaacagccg cagcgcctat
     4141 aatcaacagc aggatacctg ttaccaacaa atctgccata cgtcgctatc ctttcttttt
     4201 tatgcgtgga gacgatactc ggcgctgaaa gctttctcat ttctctggat cagcacaagc
     4261 aatgccccgg caaatgcagc cactgcgatc agtcccggaa caaaaccgtt tccaagggtt
     4321 cctgttgtta tcagcgtacc tacctgatat acgaaaaatg caactgtata accggtagct
     4381 aactgcaatg caattcctcc ccacagccat cttttatcct gcatttcaga attcatcgca
     4441 ccaagggctg caaaacaagg cggcgtgaaa agattaaaca tcagataagc aagtgccgcg
     4501 actttcgtaa gtcccataac cattgccact tcatttgctc cgccaactaa cgccagctca
     4561 tccgtatcaa taaaattcgt aagtccataa acaactgcca gggtgccgac cacgttctct
     4621 tttgcaataa aacctgtaat tgctgccgct gcaagctgcc atacgccaaa tccaagcggg
     4681 atcagaagta ctgcaaaagg agaagcaatc gaggcaagga tagacgtatg ttccattccc
     4741 tcttccacta cctggaactg ccagttaaaa ctctgcataa tctgcactgc agtgttacat
     4801 accaaaatga tcgttcctgc cttcacaatg tatgccttac cgcgggaaag catagaaagg
     4861 gttgcccgtt ttaaactcgg aattttgtat tccggaagtt caatgataaa gaacgtttta
     4921 cggaatttat atccggtaat cttttttacc aataatgcgc ccaggaaaat caacacagct
     4981 ccagccagat acatggtcgg ccctacccat gaagcgtctg cgaagaacgc ccctgcaaat
     5041 aacgcgatca ccgggagctt tgctccgcac ggcataaacg gcgtcagcat ggcggttgct
     5101 cttctttctc tttcgttccg gatggtacgg catgccatga tacccggaat agcgcatcct
     5161 gttccgatca ccatagggat tacagacttt cctgataaac caactctctt aaaaatcgga
     5221 tctaaaacca cagtagcgcg agccatatat ccgcagtctt ccaaaagagc aatcaggaaa
     5281 tacataacca ttaccagagg caggaaaccg actactgcgc ctacgcctcc aatgatgcca
     5341 tccaccaaaa gcgcctgaag gaatggattt gctccttcca ccaaacctgc aacccagccc
     5401 tgaaaagctt caatacagcc aactaaccag tccgcaatcc atgttccaac cgtagactgg
     5461 gatatgtaaa acacgccaaa cataaccact gcaaagattg ggattccaac aaatttattg
     5521 gtaagcaccg catcgatgct gtcctgctta tttttatcct ttgttaaaac ttttcttttt
     5581 tctactgctt ttacaatccc atttacaaat tcaaagcgct tcctgtcggc tgcctcaacc
     5641 tgcgctttgt cttgcagatc aatatccgcc tgcacatatg gcgctttctg cacacgtcct
     5701 tttaatgctg ctgcatgatt tacaacctct tttaatcctt catgtcctga ggttatagat
     5761 acagtctgaa taactggaca gcctaattta tccgctaatt tctttacatc aatttctgtt
     5821 tttttcttct ccgtaatatc gcttttatta agagcaacta ccaccgggat tcccagttcc
     5881 agaagctgcg ttgtaaaaaa caaacttctg ctcaaattag ttgcatccac aatattaata
     5941 atcgcatccg gattttcgtt ttttacataa ctgctggtaa tactctcctc cgatgtaaac
     6001 ggagacatag aataggctcc cggtaaatct accgcgatca gttcctccga accgccgtaa
     6061 tatcctttct tgatcggact ctcttttttc tctactgtaa ctccagccca gtttcccaca
     6121 cgttcatttc ttcctgtgag cgcattgtac atggttgttt ttccgctatt gggatttcct
     6181 gtcaacgcaa ttctcattga tgtttcctcc ttcatactca gattagcatg agctaaccca
     6241 tcgttttaaa aaaacagtga tcaaaatcac catttttata ttgaaatagc ctgcgctaaa
     6301 tcagtatcaa tattatatct gccatctttg atagatacca cgcagccgcc ttttcgatgc
     6361 gtgatcacag taatcggctc tccgctgtaa catcccagag aaaacaaaaa ttcatttaat
     6421 tcttcatcat ctgtcaatat atctttgaca atatattctt ttccttcttt agcctctgat
     6481 aaattcataa acctttggca cactccattc cttttatatc tatatattta ttttatgtat
     6541 cagataagtt attcttcatt cacgcaagtt agcttatcct aatttttata ttttgtcaag
     6601 tccattattg agttttttta tcgtcaagca tttaatttgt tacttttcac acccacgcca
     6661 ataactaaaa agagccagtg tcctgataaa tcatgtcagg gcaccggctt caacttcttt
     6721 ctttggtttt tgtgggcttt tacgttcaaa aatggctgcg atttcctgat atacatttct
     6781 ggcatctgtg cgcagcaaat gaactgtgct caggccgtca cagatatatc ctaaattttt
     6841 ttggctccgc agaaccattg cctgtttctg tttcctttta taaacccggg caagcctttc
     6901 acaaagggag ttattgacag gtacccgttt atcatgcaga aagagaagtt cgctttcttt
     6961 atactcccgc agtctgacag acagattata tccttcccgg taataattac ttggcggact
     7021 ggcttcgtat tcctccttcg ccttatcaag gatctcatcg tatctcgttt caaaatctgc
     7081 cacgataacc ggatcaggcc cctcctcttc gcacagcccg ttacggtaat ggagcatttc
     7141 ctgaaataat gcatgcatct tgtggttcca ttcaagatca ggctcatttt ccttactccc
     7201 gatcagatat ctgcagtcgt gctgtgtgca ttcctgatgc ctggtcccgt aactgtaaaa
     7261 agtacggtca tggtcatgca ccacagtccc cgtatagtct tccaggggcg tccctttgac
     7321 cccttcatgc cctttcttct ccctgcctat atacaatgcc gctccgttaa caggagatgc
     7381 aagcaccagt acctgggcac tctgtccatt tacatttgca tttgtaaaat ctgcattcat
     7441 gaccggagaa gacatgagcc tgcttatgat ctcctttttc tcggttccgg attttaatac
     7501 gaatttctca ctcaggtggt tgagcattcc gtcagatagt tccacttttc cgtcagtcag
     7561 ttctgaaagc agcgtcctta ttcttccatg tgagacattg cattcattac tgagaaggaa
     7621 ggcaagggct tttaccgtcc cgtcataatt cacctcattg gtatagcctg gcgggaatgg
     7681 ggcatgtaca cgggcaccgg taatacggct gcggtattct tttgccgtat attcaattac
     7741 ttttacggac atttcaatta caacccgctg tttgcggatc aatcatatcc atcttctcca
     7801 gcacgtaact tcattgccct attgcggaac tctctcggag tcattttata tcgtttcctg
     7861 aatactctat taaaagttct ttggctttca aaaccgcaat ccaatgcaat atttataata
     7921 gattcctttg aatcctgtaa caaagcttca gcataattca aacggacgcc attgacatat
     7981 ttactaaaat tacagtgaaa tgtctttgca aacattgata ttgtcaatat tggttgacac
     8041 attcatctca aagatatcac tgcctatgca gcctgttcca gagattggta gtatctctgg
     8101 cgcttaatta tcggaggaat cccaccgttg gcagagcaga tcctcctgtt attccagtaa
     8161 ctgatgaagt atctccaaat gagaaccttc agttcctcta tggtcatggt ctccgtattg
     8221 tagcggtcat agagaagctc gcttttcatt ctggcccaca tgctttcgca tcgggcatta
     8281 tcgtggcacc tgccaccagc actgttcatg ctttgaataa taccgtattt agcaagagcc
     8341 ttacgataag tttcactggt atattgtgtt cctctgtcag agtgtacaat agcccctcgc
     8401 agatcaggat atgacagata ggcattatcc agggtatgct cacacaaagt tgctttcatg
     8461 ttggtttcca ttgccagacc aaggacgctg gaatcaaagc agtcaaagat agctgaaaca
     8521 tacagttttc catctttagc cttgatttct gtgatgtcag ttacacattt ttcaagtggc
     8581 ttatcagatt tgaaatctcg cttcagaaga tcatctgact tacgagcttc ccgatcggcc
     8641 ttggtaatac cattcggctt acgctttggt tgatgactaa ggcctatttc atccatgacc
     8701 ctgtagacgg ttcgctcact agggatcttg agtccttccg gtttcttaag gagaagtgcc
     8761 tgatacatgc gaatgcgccc atatgtatca ttgtattcat cttcagtatg gattgctatc
     8821 atagcatcag cgagatcctg atatttccag ggccggtctt tattagcaag atatttgtag
     8881 aacccctggc ggctgacacc aagcatctgg caatagaatg agagttttcc cttaatcgcg
     8941 ccgtcttctg tctttatggc aatgaacatc attctttggt tcttgctgac ttccgacggc
     9001 tggctgcgaa aaaagcactt gcttcctcga gaaattcatt ttcctctttc agccgccgga
     9061 tttctttatc ctgatcctta acacgtttgc ggagcatagc aagttcctca gcgaggctca
     9121 ttgcactttc tggagtatgt gcaccgtcgc caatatccaa tgtgccggat ctaactgctt
     9181 tcagccatgt atggatggtt ccttctggaa tacctaattc tttggcggcc ttagcaccgc
     9241 cgatttctct ggcaagtttg acagcctgga ccttatattc gtggtcgtat tttcgttgag
     9301 tacgtgacat tgagagttcc tccttattct ctttattata catgaattct ttgagaataa
     9361 ggtgtcaact ttatttatac aacatcagtt cttcataacc atacattccg taatct
//
LOCUS       DBMDUH010000108         9393 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_3444_length_9393_cov_7.395179, whole genome shotgun sequence.
ACCESSION   DBMDUH010000108 DBMDUH010000000
VERSION     DBMDUH010000108.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 9393)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9393)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9393
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3444_length_9393_cov_7.395179"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            273..596
                     /locus_tag="ACZPCG_09450"
     CDS             273..596
                     /locus_tag="ACZPCG_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006781750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HIR1285316.1"
                     /translation="MTYEEVFTQIKSIFMEADVSGEKEHVAFQFNVTGEGEGAFYAEV
                     KEGTLCVEPYEYYDRDAVFTCTADTLFKLASGKLDPIFAFTTGKLRVEGNLEKAMKLQ
                     NFVKK"
     gene            666..2072
                     /locus_tag="ACZPCG_09455"
     CDS             666..2072
                     /locus_tag="ACZPCG_09455"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006531 - aspartate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HIR1285317.1"
                     /translation="MITRKEADSIGTMEVPMKAYYGVQTLRAKKNFHITGRPLHPEFI
                     KNLVRIKKAAAYTNMTAHILDEKIGNAIVKACDEIIEGKLHSQFIVDAIQGGAGTSAN
                     MNANEVIANRAIEILGGEKGDYTIVHPNDHVNMAQSTNDVIPSAGKLTILALLPGLLE
                     QLERLHKALRDKSIEFDDVVKMGRTQLQDAVPIRLGQSFEAYAAVIERDIRRIRSLDS
                     EIKVLNIGATAVGTAINVNPVYLFHIIRNVNLVCGTDCSQAEDLFDATQNLDGFVHTS
                     AALRVCAVNLSKICNDLRLLSSGPKAGLGEINLPPKQNGSSIMPGKINPVIPEVVSQV
                     AYNVIGNDVTVTMAAEAGQLELNAFEPVIFYKIFESIDTLRGAVETLIDNCILGITAN
                     RERCRALVESSAALATALCPALGYKKLAEIAKKCNRTGIPVRQIVLEEGLLPEEELDR
                     YLSLVDMTQAGHPVDYKL"
     gene            2333..3163
                     /locus_tag="ACZPCG_09460"
     CDS             2333..3163
                     /locus_tag="ACZPCG_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR1285318.1"
                     /translation="MYQEVSFKTEQVIYAHTRSDEADMEYKMHCHNSYEIYYMITGNV
                     EYLLEGRSYCPRPGSLLVIAPGCFHGLRVLDGNEYDRIRVHFTEDLLNGKERELLLKP
                     FGTGWGCLEEQFPIEWYFHAIEECGTYGKELQNIAIRTRILSLLAGISAIYVRSAGRV
                     HGAEGQAQEIIRYINSHLRDTLTVEGLAEQFFMSRNHLTSIFRQATGTTVARYILYKR
                     MTIARKELAMGTPAAEAASRAGFGDYSSFFRAYRKMFGCAPTDKTAMELPDMDKSGDF
                     "
     gene            3336..4679
                     /locus_tag="ACZPCG_09465"
     CDS             3336..4679
                     /locus_tag="ACZPCG_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009262349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HIR1285319.1"
                     /translation="MNVQGRKKHMTSAQIIIFGFMVVIFMGALLLMLPISTKGPGGAP
                     FLDALFTSTSAVCVTGLIVHDTATYWTEFGQFVIISLIQVGGMGVVTVAVAVAMVSGK
                     KIGLRERSTMQDAISAPKVGGIVRFTGFILKGTFIVELTGAVLMAPQFIKDFGFVKGI
                     WYAVFHSISAFCNAGFDLMGVREPFSSLTHYVGHPVINFAIMMLIVVGGLGFLTWEDI
                     WANRRCWRKYRMQSKVILTTTALLILIPAVIFYFLEFNGPEWQGWGARIWASLFQSVT
                     PRTAGFNTVDLTAMSEPGQLLTAILMLIGGSPGSTAGGMKTTTLAVLLLSGWAVFYRK
                     EDTQCYGRRISEETVRYAAVVMMMYITLCIGGALAISYLEGLPILTCIYETASAIATV
                     GLTLGITPELGTTSRIILILLMYCGRVGGLTLVYAAISGVKPHVSRLPQEKITVG"
     gene            4698..5345
                     /locus_tag="ACZPCG_09470"
     CDS             4698..5345
                     /locus_tag="ACZPCG_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005330390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIR1285320.1"
                     /translation="MKSILLIGLGRFGRHVAEKLYELDHQVMAVDKQEDRVEAVMSYV
                     TNAQIGDSTNMEFLETLGVSNFDVCIVAIASDFQSSLETTAYLKDLGAKMVVSRASRD
                     VHARFLLRNGADEIVYPEKQVANWTAIRYSSEHIFDYVQLSDTHAIFEIEMPQNWVGK
                     TIGQVDIRKKFNINIMALKRDGKLDMAIRPDTMLTDDATMLVLGESRDIQKCFKI"
     gene            5342..6427
                     /locus_tag="ACZPCG_09475"
     CDS             5342..6427
                     /locus_tag="ACZPCG_09475"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1285321.1"
                     /translation="MKPFSRRTHSIIRDGGRAAGILIAATLVGLLFSGLKIPEANIIA
                     VYILGVLIISIITSSWMYSFLASVISVLAFNFLFTIPRFTLRAYDPSYPVTFGIMFLV
                     ALITGSLASRMKEYARESAQAAMQIEKEQLRADLLRSISHDLRTPLTSISGNASNLLS
                     NENEFSQETRMQIYGDIYDDSMWLIKLVENLLSVTRIEDGRMDLRMSAELMDEVIAEA
                     MRHTDRNRDGRKIEVSSDEEFILGKMDARLIVQVVINLVDNAVKYTPEGAQIRIHTGK
                     KDGMVVVSVSDTGPGIPDEQKSKVFDMFYTGTNRAADGRRSLGLGLGLCRSIIRAHGG
                     EIWVSDNKPQGAVFTFTLPAEEVTLHE"
     gene            6420..7121
                     /locus_tag="ACZPCG_09480"
     CDS             6420..7121
                     /locus_tag="ACZPCG_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR1285322.1"
                     /translation="MNKPLILVVEDDAPVRNLITTTLKANDYRFQTAANGEAGVLATA
                     SQNPDIILLDLGLPDMDGMEVIKRVRSWSNVPIIVISARSEDTDKIDALDAGADDYLT
                     KPFSVEELLARLRVTQRRLAVMNGDGAGRESVFTNGRLRIDYGAGCAYLGEKEMHLTP
                     IEYKLLCLMAQNVGKVLTHTYITQKVWGSSWDNDIASLRVFMATLRKKLESEPDSPQY
                     IQTHIGVGYRMMKVE"
     gene            7271..8605
                     /locus_tag="ACZPCG_09485"
     CDS             7271..8605
                     /locus_tag="ACZPCG_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1285323.1"
                     /translation="MTKDMTTGKIMPMLVQFTVPLVLGNLFQLTYNAVDSIIVGHFVG
                     KEALAAVGICNPITTLMILFLNGLCMGAAILMGTQYGARDYDTLHRQISTTMISGMVF
                     SIILTLICVIFAEPILMLMQVDPSIMQMTKEYLRIIFWGLMFTFLYNFFSSTLRALGD
                     SNTPLYFLIISAVLNIFGDLFFVVVLRAGSNGCAIATVISEALCCLFCMIYIQKKVPM
                     LRLGKKWLVFDRKLLKRTISYGWASAMQQATVQLGKLGIQAIVNTMGVSVAAAFAVVN
                     RIDDFAYTPEQNIAHAMTALMAQNKGARKKERMREGFRCGMKLELIYGVAVFAVCFLF
                     ARPLMLLFVKDEEVIRHGVSYLHLIAAMYLLPAATNGIQGYFRGIGDLKITLISSFIN
                     MGVRVLAALPLVFLMGMGIEALPLAYMAGWIGMLIAEVPLLVRTYRKGMDGN"
     gene            9010..9081
                     /locus_tag="ACZPCG_09490"
     tRNA            9010..9081
                     /locus_tag="ACZPCG_09490"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9043..9045,aa:Arg,seq:cct)
BASE COUNT         2473 a         1908 c         2600 g         2412 t
ORIGIN      
        1 ggcgccggaa accgggcgct gatcttagta tgtcccggtt tcataaggct ccctttggga
       61 atgaacggaa gaaaaggaat ttgccgcgtc tattctattt cccgccgtca ataataaatt
      121 aagaggaaga gttagttttg tgcttcagaa acagaaaatg ccttccgttt tgtgcggaga
      181 gtataacata tccgattttc ggctgtctgg aaaataaagg gaaatcatgg tatactaaag
      241 aagtgtaaaa aacaggaaaa ggagatacag gtatgacgta cgaggaagta tttacacaga
      301 ttaaaagtat ttttatggag gcagatgtaa gcggggaaaa ggagcatgtg gcattccagt
      361 ttaatgtcac aggagaaggg gaaggagcat tttacgcaga agtaaaagag ggtactctct
      421 gtgtggaacc ctatgaatat tatgacaggg acgcagtgtt tacctgtact gcagatacgc
      481 tttttaagct ggccagtgga aaacttgatc cgatttttgc ctttaccaca ggaaaattga
      541 gagttgaggg aaatctggaa aaagcaatga aactgcagaa ttttgtaaaa aagtgatatg
      601 ctccagatag gagccggagt aaaggtggtt tttgacacta cctgcaaaac aggagggaat
      661 atattatgat aaccagaaaa gaagcggatt ccataggcac aatggaggtg cccatgaagg
      721 cgtattatgg agtacagaca ttacgcgcga agaagaattt tcacattaca ggacggccgc
      781 tgcatccgga atttataaaa aatctggtga gaataaaaaa ggccgcggct tataccaata
      841 tgacggccca tatactggac gagaagattg gaaatgccat tgtaaaggcc tgtgatgaga
      901 tcatagaggg aaagctccat tctcagttta ttgtagacgc cattcaggga ggcgcaggca
      961 cctcggccaa tatgaatgct aacgaggtca tagccaaccg ggcgattgag atcctgggcg
     1021 gtgaaaaggg ggattatacg attgttcatc ccaatgacca tgtaaatatg gcccagtcta
     1081 ccaatgatgt gatccccagt gcaggaaagc ttacgatcct ggcgctgctt ccgggccttt
     1141 tggaacagct ggaacggctg cacaaggctc tgagggacaa atccattgaa tttgacgatg
     1201 tggtaaagat gggacggacc cagcttcagg atgcggtgcc gatccgcctg gggcagtcct
     1261 ttgaagccta tgcggcagtg atcgagagag atatccggcg gatacgctcc ctggattcag
     1321 agattaaggt gttgaatatc ggagctactg cggtgggaac ggcgatcaat gtaaacccgg
     1381 tgtacttatt tcacattatc cgaaatgtaa acctcgtctg tggcacagac tgcagccagg
     1441 cagaggatct gtttgacgcc acccagaatc tggacggctt tgtacatacc tctgcggctc
     1501 tcagagtctg tgcggtaaat ctgtctaaga tctgcaatga tctgcgtctg ctctccagcg
     1561 gccccaaagc aggactggga gagatcaatc tgcccccgaa gcagaacggc tcttccatta
     1621 tgccgggaaa gatcaatccg gttatcccgg aggtggtttc ccaggttgcc tacaatgtga
     1681 tcggaaatga cgtaaccgtt actatggctg cagaggcagg acagctggaa ttaaatgctt
     1741 ttgagccggt gattttctat aagatttttg agtcgatcga taccttaaga ggggcggtgg
     1801 agacgctgat cgataactgt attctcggta ttacggcgaa cagagagcgc tgccgtgctc
     1861 tggtagagtc cagtgcggct ttggctacag ccctttgtcc ggctctggga tataaaaaat
     1921 tggccgaaat tgcaaaaaaa tgcaatcgaa ccgggattcc ggtgcgccag atcgtacttg
     1981 aggagggact gctgccggaa gaagaactgg atcgttattt aagtctggtg gatatgaccc
     2041 aggcgggcca tccggtggat tacaaattat aaaactgctt aaacagcaaa agaccaggta
     2101 ctggggatac ctggtctttt ctatgtgtat aaaggggggg ataaaatgtg tgaaaggggg
     2161 aggacccttt cggttttgtt tacagttagg attatagtat attggggtaa ggatttccat
     2221 tgtgaaatga aaaagaaata ttgcaaaatg cacaaactct gataaactga tagataacga
     2281 aaaatgtgcg gccgaaggaa gggacggaca gaaaaagtca ggaggaaatg aggtgtacca
     2341 ggaggtcagt tttaagacag agcaggtgat ctatgcccat acccgcagtg atgaggcgga
     2401 catggagtat aagatgcact gccataacag ctatgagatt tattatatga taaccgggaa
     2461 tgtggagtat ctgctggaag ggcggtccta ttgtccccgt ccgggaagcc ttttagtgat
     2521 cgcacctggc tgttttcatg gtctgagagt gctggatgga aatgaatatg accggatccg
     2581 ggttcatttt acagaggatc ttttaaatgg aaaggagcgg gagcttctgt taaagccctt
     2641 tggaacggga tggggctgtc ttgaagagca gttccccatt gaatggtatt ttcatgcaat
     2701 cgaagagtgc ggtacttacg ggaaggagct gcagaatatt gcaattcgca cacggatcct
     2761 gtcgcttttg gcgggtattt ctgcaatata cgtccgaagt gcgggacgcg ttcatggagc
     2821 agagggacag gcccaggaga taatccggta tattaacagc catctgaggg atacgctgac
     2881 ggtagagggg ctggcagagc agttttttat gagccgaaat catctgacct ccatattccg
     2941 ccaggcaaca ggaactacgg tagcccgtta tattctttat aagcgcatga ctattgcaag
     3001 aaaagaactg gcaatgggga ctccggcggc tgaggctgca tccagagccg gctttggaga
     3061 ttattccagc tttttcagag cctatcggaa gatgttcggc tgcgctccta ccgataagac
     3121 ggcgatggaa cttcccgata tggataagag cggagatttt taggcagttt cctaatgggg
     3181 cctttatgcc gtctttatac aatctttata ccagcgtttt tacttttaac acaatctttt
     3241 tgaatgatgt ggcataatta gcaattacag agacaatata aagaccgtat aaagagacgg
     3301 tttttatggt gtccgttaac cggagggata ttatgatgaa tgttcagggc agaaaaaaac
     3361 atatgacgtc cgctcagatc attattttcg gttttatggt agtgatcttt atgggagcac
     3421 tgctcctcat gcttcccata tcaaccaaag gaccaggggg agcgcccttt ttagacgcgc
     3481 tgtttacctc tacatctgcc gtatgtgtta cagggctgat cgtacatgat actgcgacct
     3541 actggacgga atttggccag tttgtgatca tttcgctgat tcaggtcggc ggtatgggcg
     3601 tagtgactgt ggctgtggcc gtggctatgg tgtctggaaa aaaaatcggc ctgagggaac
     3661 gaagtaccat gcaggatgct atttctgcac ccaaggtagg agggatcgtc cgttttacag
     3721 gctttatcct caaaggtacg tttatcgttg agctgacggg cgctgtcttg atggcgccgc
     3781 agtttattaa ggatttcggc tttgttaagg gaatctggta tgcagtattc cactcgattt
     3841 ccgcattctg caacgcaggt tttgacctta tgggcgtaag agaacccttt tcctctctga
     3901 cccattatgt gggccatccg gtcatcaatt ttgccattat gatgctgatt gtagtgggcg
     3961 gtctgggctt tctcacatgg gaggatatat gggccaaccg gcggtgctgg agaaaataca
     4021 ggatgcagag caaggtgatt ctcaccacaa cggctctttt gatcctgatc ccggcagtga
     4081 ttttttattt tcttgaattt aacgggcctg agtggcaggg atggggcgcg cgtatctggg
     4141 cttctttatt ccagtctgta acgcccagga ctgcgggatt taacacggtg gatctgacag
     4201 ccatgagcga gccgggacag cttcttacag cgattttgat gctcatagga ggttcgccgg
     4261 ggtctacggc aggcggtatg aaaacgacta ctctggcagt gcttttgctg tccggctggg
     4321 cagtatttta ccgcaaggag gatacgcagt gctacgggcg gcgtatttca gaggaaaccg
     4381 tgcgttatgc ggcggttgta atgatgatgt atatcacact ctgtatcgga ggcgcgcttg
     4441 ctatcagtta tctggaaggc cttcctatct taacctgtat ttacgaaaca gcctctgcca
     4501 ttgctacggt aggattgacc cttggcatta cgccggagct gggaacgaca tcacggatca
     4561 tactgatcct tttaatgtat tgcggaagag taggcggact gacactggtc tatgcggcta
     4621 tttccggagt gaagcctcat gtttcaaggt tgccccagga aaagattact gtgggataaa
     4681 agaaagggaa atgacttatg aaatcaattt tacttattgg actgggccgt ttcggccgtc
     4741 atgtggcaga aaaattatac gagctggatc atcaggttat ggcggtagat aagcaggagg
     4801 accgcgtgga ggcggttatg tcatatgtaa ccaatgccca gatcggagac agcaccaata
     4861 tggagttttt ggaaacactt ggcgtaagca attttgatgt gtgcattgtc gccattgcca
     4921 gcgacttcca gagctctctg gagacaacgg cttatttaaa ggatctgggg gccaagatgg
     4981 tggtttcccg tgcttccaga gatgtacacg cccggttcct gctgcggaat ggtgcagatg
     5041 agatcgtcta cccagaaaag caggtggcaa actggacagc tatccgctac agctcagagc
     5101 atatatttga ctatgtgcag ctcagtgaca ctcacgctat ttttgagatt gaaatgcctc
     5161 agaactgggt gggaaagacc atcggacagg tagacatacg aaagaagttc aatatcaata
     5221 tcatggctct gaagcgggat ggaaagctgg atatggcgat ccggccagat accatgctta
     5281 ccgatgatgc gaccatgctg gttctgggtg agagccgtga tatccagaaa tgttttaaga
     5341 tatgaaacca ttttcccgta gaacacacag cattataaga gatgggggac gggctgcggg
     5401 aattctgatc gcggctacgc tggtgggact ccttttttca gggcttaaga tcccggaggc
     5461 gaatattatc gccgtgtaca tcctgggtgt gctgattata tctattatta caagcagctg
     5521 gatgtacagt tttctggcct ctgtgatcag cgtactggcc tttaattttt tgtttacaat
     5581 cccgcgtttt accctgcggg cctatgaccc cagctatccg gtgacctttg gcatcatgtt
     5641 tctggtagcc cttattaccg gttctctggc ctcccggatg aaggaatatg ccagggagtc
     5701 cgcccaggcg gccatgcaga ttgaaaagga gcagcttcgg gctgaccttc ttcggtccat
     5761 ttcccatgat ctgcgcactc ctcttacctc tatttccgga aatgccagca acctgctttc
     5821 caatgaaaat gaattcagcc aagagacgcg gatgcagatc tatggggaca tttatgatga
     5881 ttccatgtgg ctgatcaagc tggtggaaaa tctgctgtct gtcacacgga ttgaggacgg
     5941 acggatggat cttcggatgt ctgcagagct tatggatgag gtcattgccg aggcgatgcg
     6001 ccatacggat cggaaccggg acggacggaa gatcgaggta agcagtgatg aggagtttat
     6061 tttgggaaag atggatgcca ggctgattgt ccaggtagtg atcaatctgg tggacaatgc
     6121 tgtaaaatat acgcctgagg gagcccaaat tcggatccat acaggaaaaa aggacggtat
     6181 ggtggtggtg tcggtcagcg atacaggtcc tggaataccg gatgaacaga aatcaaaagt
     6241 atttgatatg ttttatacag gaacaaaccg ggcggcggac ggacgcagga gcctgggact
     6301 ggggctggga ctgtgcagat ccattatccg ggcgcacggg ggagaaatat gggtatcaga
     6361 caataagccc cagggggctg tatttacatt tacactgcct gcagaggagg taacactgca
     6421 tgaataaacc attaatttta gtggtcgagg atgacgctcc ggtgcgcaat ttgatcacta
     6481 ccacattaaa agcaaacgat taccggttcc agacagcagc aaacggagaa gcgggagttc
     6541 tggcaaccgc ttcccagaat cccgatatta tcctgctgga tctgggactg cctgatatgg
     6601 acggcatgga ggttatcaaa agggtacgca gctggtcgaa tgttcccatt attgtgatca
     6661 gtgccagaag cgaggatacg gataagatcg acgctctgga tgcgggggca gatgattatc
     6721 tgacgaagcc cttttccgtt gaggagcttc ttgccagact gcgcgtcacc cagaggcgtc
     6781 tggcagttat gaacggcgac ggggcgggaa gagaatccgt atttacaaac ggaaggctgc
     6841 ggatcgacta tggagcaggc tgcgcttatc tgggagagaa ggaaatgcac ttaacgccga
     6901 tcgagtacaa gctgctctgc ctcatggccc agaacgtggg aaaggtcctg acccatactt
     6961 atatcacgca gaaggtatgg ggaagcagct gggataatga tatcgcctcc ctgcgggtat
     7021 ttatggcaac tctgcgtaaa aagctggaga gcgagcccga ttccccccag tatatccaga
     7081 cccatatcgg agtggggtat cggatgatga aggtggaata aagctgcgca ccggacttca
     7141 ggatacgccg gaggcggtgc aaaatgcaga gacgagaagg agttttttct tgacattctc
     7201 ctgaaatgca ctaatattgt aagtgtttac attttatagt atgaaagaga atgacaaggg
     7261 agaacacagt atgacaaagg atatgacgac cggaaaaata atgcccatgc tggtgcagtt
     7321 cacagttccg cttgtgctgg ggaatttatt ccagctgaca tacaatgcgg tggacagtat
     7381 catcgtgggc cattttgttg gaaaagaggc actggcggct gtgggaatct gcaatcccat
     7441 taccactctg atgatccttt ttctcaacgg actgtgcatg ggagcagcca tccttatggg
     7501 aacgcagtac ggagccaggg attatgatac gcttcaccgg cagatcagta caaccatgat
     7561 atcgggaatg gtattttcca tcattctgac cctgatctgt gttatatttg cagaaccgat
     7621 cctcatgctc atgcaggttg atccgtcgat catgcagatg acaaaggagt atctgcggat
     7681 cattttctgg ggtctgatgt ttaccttttt atataacttt ttttccagta ctttaagggc
     7741 tctgggggac agcaatacgc ccctttattt tttgattatc agtgcagtat taaacatttt
     7801 cggcgatctg ttttttgtag tcgtattaag ggccggaagc aacggctgcg ccattgccac
     7861 tgtaatcagc gaggctcttt gctgcctgtt ttgtatgatt tatatacaaa agaaggtacc
     7921 aatgctgcgg ctggggaaaa aatggctggt gtttgaccgg aagctgctga agcgcacgat
     7981 ttcctatgga tgggcttccg ccatgcagca ggctaccgtt cagctgggaa agctgggaat
     8041 acaggctatt gtaaatacca tgggcgtatc ggtggcagct gcttttgcag tcgtaaaccg
     8101 gatcgatgat ttcgcctata cgccggagca gaatatcgcc cacgctatga ccgctcttat
     8161 ggcccagaat aaaggcgcaa gaaagaagga acgtatgagg gaaggcttcc gctgcggaat
     8221 gaagctggag ctgatttacg gcgtggctgt gtttgccgtc tgctttctct ttgcacggcc
     8281 cttgatgctg ctctttgtaa aggatgagga ggtcatccgc catggagtaa gctatctcca
     8341 tctgatcgca gccatgtatc tgcttcccgc agctactaac ggtatccagg gctatttcag
     8401 agggattggc gatttgaaga ttaccctcat cagcagcttt atcaatatgg gtgtgcgggt
     8461 actggcagca ctgccgctgg tattcctgat gggaatggga attgaggccc ttcctctggc
     8521 ctatatggca ggctggatcg gaatgctgat cgcagaggtt ccgctgctgg tgcggactta
     8581 ccggaaggga atggatggga attgatactt gatttttgga tggaaatcat gtatgatata
     8641 aaacaggctg tgagagaaaa tgcagcctgt cttgtctttt ggctttgagg ctgagagatg
     8701 agtggagttg tttccgtagc tcagttggat agagcgaccg cttcctaagg gcttgttgga
     8761 gcaatcgccc aacactgaaa attttatata cgtccatgta cctcagtagg atagagggtt
     8821 cgcctcctaa ggtagaacca tggcggaacc cttttgaaga aaaagggtag cgtttgaggg
     8881 tcacttaagg aagcgaaatc tgaaaaatca gaagagatag gaaagagata attcatcacg
     8941 aaagtgttag agttataaat gatggcagcc ggagccaaag ggtcgaaaaa gcccggaaat
     9001 acggcatttg tctccttagt taaatggata taacggggtc ctcctaagac tctattgtag
     9061 gttcgattcc tataggagac gctcatttta agcagtaatg attgaaaaag tcattgctgc
     9121 tttttttgta tcttgggtgg aaatttccac ccggaattga aaaaacagaa ggatttcatc
     9181 cacccaaaat aagattttgc cacccatttg aaaattatga ttttccaccc ggatgtgtgg
     9241 catatggtgt agggaagaaa aagggttgtc aatggatgca aatttggttt tcttgtctgt
     9301 acgattcaga cctgagaaag atagatgctc agattagatg taacaagtct agtttgggca
     9361 ttttttcgtc cagaagaaaa ggaggattac atg
//
LOCUS       DBMDUH010000109         9241 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_3493_length_9241_cov_6.761488, whole genome shotgun sequence.
ACCESSION   DBMDUH010000109 DBMDUH010000000
VERSION     DBMDUH010000109.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 9241)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9241)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9241
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3493_length_9241_cov_6.761488"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(1..1542)
                     /gene="guaA"
                     /locus_tag="ACZPCG_09495"
     CDS             complement(1..1542)
                     /gene="guaA"
                     /locus_tag="ACZPCG_09495"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002596053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HIR1285324.1"
                     /translation="MAREMIIVLDFGGQYNQLIARRVRECNVYCEVHPYNMSLDQIRE
                     MNPKGIIFTGGPDVVFEDGAPRCSKEIFELGIPVLGICYGAQLMSYLLDGVVKKAVVS
                     EYGHTEVDVDTASQLFDGVSPKTVCWMSHGVYIAEVPAGFRITAHTESCPVAGMECAE
                     KNFYAVQFHPEVVHTKEGTKMLSNFVYNVCGCSGDWKMDSFVDTTIKALREKIGSGKV
                     LCALSGGVDSSVAAVMLSKAIGKQLTCVFVDHGLLRKNEGDEVEAVFGPEGPYDLNFI
                     RVNAQERFYERLKGVEEPEAKRKIIGEEFIRVFEEEAKKIGAVDYLVQGTIYPDVIES
                     GLGKSAVIKSHHNVGGLPEHVDFKELVEPLRLLFKDEVRKAGLELGIPENLVFRQPFP
                     GPGLGVRIIGEVTADKVRIVQDADAIYREEIAKAGADKNLGQYFAALTNMRSVGCMGD
                     ERTYDYAIALRAVLTSDFMTAESAQIPWEVLAKVTSRIVNEVKGVNRVLYDCTGKPPA
                     TIEFE"
     gene            1751..2716
                     /locus_tag="ACZPCG_09500"
     CDS             1751..2716
                     /locus_tag="ACZPCG_09500"
                     /inference="COORDINATES: protein motif:HMM:NF022337.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CFI-box-CTERM domain-containing protein"
                     /protein_id="HIR1285325.1"
                     /translation="MKCKICGSESGSYPLCRTCYAKREAGEIIKCVKCGSWHYAGSPC
                     HCEEGFQASRTSSEAELPFLYEAKPSLVTKTETAYLNCIKSFLPDTCLIQAQANLASF
                     IRRTDGAKYQNELFRNVDFIITDLSYRPLLVIEINDQTHRLPERRERDKKVACICEEA
                     GIPLINLWTSYGVNEEYIKKKITQTLASLPVERIHHFAPVHEETAQPVLESPLPGASA
                     ASSSHAAPKQPQTSANKQTQPASQKEGCYIATCVYGSYDCPQVRLLRRFRDEHMKKTP
                     AGRIFIRLYYAVSPVLVRCFGKNPAFHVFWRHILDSLIRRLESGR"
     gene            complement(2741..3592)
                     /locus_tag="ACZPCG_09505"
     CDS             complement(2741..3592)
                     /locus_tag="ACZPCG_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003499241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HIR1285326.1"
                     /translation="MFERNAFLRAYNIDEADFKAANVPWEELAAIYDDYLSIEGKLRG
                     IGKEFVNDYLYDIERAGIHSYRYRTKSPGHLLEKIIRKRSEHPGRFDHIDRTNYHKYI
                     TDLIGIRVFFLYREDWIYFHNYITTVFENDPENYVEDRIRDFDEDPEHFYIAERPKVY
                     RRNGDTRIYDEELIDIKSDGIYRSLHYIIKYRGYYVEIQGRTLFEEGWSEIDHDIVYP
                     YFKDDEMLKDFSTLLNRLSGMADEMSSYFRRMKSVREEPGKNRKERRALTGGGNIWTE
                     QEERERK"
     gene            complement(3719..4102)
                     /gene="gloA2"
                     /locus_tag="ACZPCG_09510"
     CDS             complement(3719..4102)
                     /gene="gloA2"
                     /locus_tag="ACZPCG_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMU1112c/YaeR family gloxylase I-like
                     metalloprotein"
                     /protein_id="HIR1285327.1"
                     /translation="MKFSTIHHIAIIVSDYEKSRDFYVNKLGFSVIRENYRPDRGDWK
                     LDLRINDTTELEIFGVSNPPKRVTRPEAAGLRHLAFYTEDIEKDVKELKEKGIFVESI
                     RLDEFTGKKMTFFADPDGLPLELHE"
     gene            complement(4131..4853)
                     /locus_tag="ACZPCG_09515"
     CDS             complement(4131..4853)
                     /locus_tag="ACZPCG_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007718476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1285328.1"
                     /translation="MEQVNLYVGAQIRRYRKNRQMTLQQLADRIHKSRASVSKYETGE
                     VSLDIVTLTEIARVLEVQPGQLVDLPVQEEEGARLPSANSGISPFFQARRLYFYFYDG
                     RYKRLKDGIIDIRETKEAGSYEADLSVSAVNPSGRSSKLYYTGKVIYSDMLIRFSFVN
                     RYNPLEEDLLYIFNPLELRDSTDGLLCGISSADLMPCAFKCLVTLHPQEHTEEFKKRL
                     LITRRELQQWQKLNMLMVDNIG"
     gene            5135..6550
                     /locus_tag="ACZPCG_09520"
     CDS             5135..6550
                     /locus_tag="ACZPCG_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HIR1285329.1"
                     /translation="MEAKKKSSFTGSLGFVLAAAGSAVGVGNIWRFPYLAAKDGGGLF
                     LIIYLALVLTFGFTLLVTDVAIGRRTKTNALHAFGKMQKKWSFLGYLTFCVPAIIMTY
                     YSVIGGWILKYLAVYLTGAGKAAAQDGYFTDFITSPVSPVVFMLIFLCFTALVVYRGV
                     EKGIEKFSRIVMPGLLLMIIGIGLFSLTLSSTGADGTERTGLQGLTVYIMPNLDGVTP
                     ARFLEILLDAMSQLFFSLSVSMGIMITYGSYVKKDVNLSKSLSQIEIFDTTVAFLAGL
                     MIIPAVYVFFGTEGMASGPSLMFVSLPKVFEAMGPLGGVLGMIFFLMVVFAALTSSVS
                     ILETLVANCMEIFHSTRKKTSLVIALLSAAASVIICMGYNVFYFEMKLPNGASAQLLD
                     LMDYISNSFLMPFISLLTCIFVGWIIRPTWICEEMEASGHTFKRKKMYAFMIRYVTPI
                     IMAMLFLQSAGVFEIIKNMFG"
     gene            complement(6637..7800)
                     /locus_tag="ACZPCG_09525"
     CDS             complement(6637..7800)
                     /locus_tag="ACZPCG_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1285330.1"
                     /translation="MGRGKKILPAVMALTLAAGSITGCSASSAKTETAKESMQSQAET
                     KTAEQSTQAEKTSEAADAAGEREITDMAGRTVVVPDEIETVFSSSTVTAIFMYTLAPD
                     KLLGWNYELNELEKSIILEEYHDLPNFGMGDSINYEAVIAADPTIAVNVGTINDKMIS
                     DCDKLSKSLGVPVVAVDGDLSASAEAYRFMGELLGEEEQAEKLASYAEKTFADIEKME
                     VPEDKKVRIYYGNGEDSLETAPAGSAHGQIIDMVNAVNVADLEMGEGSRVQISAEQLL
                     AWDPDVIVVNGEPKADTSGASAAEAILANPDYASLKAVQDQQVYGTPNTPFSWMDRPM
                     GPNRIVGIRWLSGLIYPEYLNYNVDEEVKEFFDLFYHVQLTDEKLENIYSGTV"
     gene            7941..8978
                     /locus_tag="ACZPCG_09530"
     CDS             7941..8978
                     /locus_tag="ACZPCG_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HIR1285331.1"
                     /translation="MLNHHFEVKQEAAQRRRYGLLITGLLIFLFTAVLLSFWVGYYPL
                     SPSQVVTAFLSRFGYEGEILPQAVTIFWNIRLPRILSAVFIGASLSTAGAAYQGMFRN
                     PLVSPDILGVSSGASLGAAFAILFGASNWLIQLSAFAGGTAAVAASYLISRRSVHSIT
                     LSLVLTGSMIMALCNAGVTMIKYVADPNDVLQQITFWLMGSLTKTDTRSFLWSLIPMS
                     AGLVIIFLLRWRINLLTLDEEEAKSLGINIRLYRLILIAASTLLSAASVCLGGLIGWV
                     GLMIPHMARALVGADYGRLIPASAMLGATYLVLMDDLARSLLSMELPLGVVTSIMGAP
                     FFVSLIIQKKE"
     gene            8985..>9241
                     /locus_tag="ACZPCG_09535"
     CDS             8985..>9241
                     /locus_tag="ACZPCG_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1285332.1"
                     /translation="MLLQLQNVSGGYGKGDIVKNVSCEADEGDILCLAGPNGCGKTTL
                     FRLIMGSIPLSGGRILIDGQDHLKLSPRELANRIAYIPQYHS"
BASE COUNT         2232 a         2512 c         1935 g         2562 t
ORIGIN      
        1 ttattcaaac tcaatcgtcg ccggcggctt ccccgtacag tcatacagca cgcggttcac
       61 gcctttgacc tcatttacaa tcctgctggt gacctttgcc aggacttccc aggggatctg
      121 tgcggactct gcggtcatga aatcgcttgt gagcacggca cggagggcga tggcgtagtc
      181 ataggttctt tcatctccca tgcagccaac ggagcgcata ttggtaaggg cggcgaagta
      241 ctggcccaga ttcttgtctg ctcctgcctt tgcaatctcc tcacggtaga tagcgtcagc
      301 gtcctgtacg atccgcacct tgtctgccgt tacctctccg atgatgcgca cgcccagacc
      361 ggggccgggg aatggctgac ggaataccag attttcagga atgccaagct ccagtcctgc
      421 cttgcgtacc tcatccttaa agagcagtct taagggctct accagctcct tgaaatcaac
      481 gtgctccgga aggccgccta cgttatgatg ggatttgatc actgcggact ttccaagtcc
      541 ggactcaatc acatcggggt aaatggttcc ctgaaccaga tagtccactg ctccgatctt
      601 tttggcttct tcctcaaata cgcggataaa ttcctcgcca atgatcttgc gctttgcctc
      661 aggctcctcc acgcccttta atctctcgta aaaacgctcc tgtgcattca cgcggatgaa
      721 gtttaagtca taaggacctt cgggaccgaa taccgcttct acctcgtcgc cttcgttttt
      781 acggagcaga ccgtggtcta cgaaaacaca ggtcagctgc tttccaatgg ccttggaaag
      841 cataacagcc gcaaccgagg aatcaacgcc gccggaaaga gcgcagagta ccttaccgga
      901 accgattttt tcccgaagag ccttgattgt cgtatctacg aaggaatcca ttttccagtc
      961 gccggagcag ccgcagacat tatatacaaa gttggaaagc atctttgtgc cttctttcgt
     1021 gtgaacgacc tcaggatgga actgaactgc atagaaattc ttctcggcac actccatacc
     1081 tgctaccgga caggactctg tatgagcagt gatgcggaaa ccggcaggaa cctcagcaat
     1141 atatacgccg tggctcatcc agcagacggt tttgggagat acaccgtcaa aaagctgaga
     1201 ggctgtatcc acgtctactt ctgtatgtcc atattcgctt acaacggctt ttttaacaac
     1261 gccgtccaac aggtagctca tcagctgagc gccatagcag attcccagaa ccggaatacc
     1321 cagttcaaaa atctccttag agcagcgggg tgctccatcc tcaaatacca catcgggacc
     1381 gccggtaaaa atgattccct tgggattcat ctcccggatc tgatccagac tcatgttata
     1441 gggatgtacc tcgcagtaaa cattacattc acggacacgg cgggcaatca gctggttata
     1501 ctggccgcca aagtctaaaa caataatcat ctctcttgcc atgagattat ctcctctatc
     1561 tgttatttcc ttataaatcg aaagagattg tacattgaaa gtacaatctc cttttatctt
     1621 tggtacacac acattataaa ggatggagga ttaaatagca agatattttc tcaagttttt
     1681 tcctctgatc tggcagtttt ttcccgactg tggtaaaatt ttcttataaa atataaggag
     1741 ggctgtgcct atgaaatgta aaatatgcgg cagtgaaagc ggaagctatc ccttatgcag
     1801 gacctgctat gccaaacggg aagctggtga gatcataaaa tgcgtcaaat gcggaagctg
     1861 gcactatgcc ggttccccct gccactgtga agaaggcttc caggcatccc ggacctcctc
     1921 tgaagcggag cttccatttt tatatgaagc aaaaccatct cttgtgacga aaactgagac
     1981 ggcatactta aactgtatta aatcctttct tcccgatacc tgcctgatcc aggctcaggc
     2041 caatctggcc tcatttatca gaagaacgga tggggccaaa tatcagaatg agctctttcg
     2101 aaatgtagat ttcatcatta cagatctgtc ataccgccct cttcttgtaa tagagatcaa
     2161 tgaccagacc caccggctgc ctgagcggag ggaacgggac aaaaaggtgg cctgcatctg
     2221 tgaggaagcc ggaatcccct tgatcaacct gtggacctcc tacggcgtaa atgaagagta
     2281 tataaaaaag aaaatcacac aaacccttgc ttctcttccc gttgagcgta tccaccactt
     2341 tgcccctgta cacgaagaga ctgcacagcc ggttttagaa tcgcctctgc cgggagcatc
     2401 tgctgcttca tccagccacg ctgcaccaaa gcagcctcag acctccgcca acaaacagac
     2461 gcagcccgcc tcccaaaagg aaggctgcta catcgcaacc tgcgtctatg gctcctatga
     2521 ctgccctcag gtccggctcc ttcgccgttt cagagacgaa catatgaaga aaactcctgc
     2581 cggacgtatc tttatacgat tatactatgc cgtcagccca gtcctggtcc gctgctttgg
     2641 aaaaaatcct gcctttcatg tcttctggag acacattctt gactccctga tccgccggct
     2701 ggaatccggc agataaaacc aaaactagac aatccggccc ctattttctc tcacgttcct
     2761 cctgctccgt ccagatattt ccgccgcctg taagagcccg tctctccttc cggttcttcc
     2821 ccggctcttc ccggacgctc ttcatccggc ggaaatagga gctcatttca tctgccattc
     2881 cggacaggcg gtttaacagc gtggaaaaat cctttaacat ttcatcatct ttaaaatagg
     2941 gatatacgat atcgtgatcg atctcactcc acccctcttc aaacagggtt cttccctgga
     3001 tctccacata gtagcccctg tatttgataa tataatgaag cgaccggtaa attccatccg
     3061 atttaatgtc gatcagctct tcatcgtaaa tccgggtatc tccgtttctc cggtaaacct
     3121 ttggccgttc ggcaatgtaa aaatgctccg gatcctcgtc aaaatcccgg atacgatcct
     3181 ctacatagtt ctcaggatca ttttcaaaca ccgttgtaat ataattgtga aaataaatcc
     3241 agtcctcccg gtacagaaaa aatacgcgga ttccgatcag atcggtaata tacttgtgat
     3301 aattagtccg gtcaatatga tcaaatctcc ccgggtgctc gctgcgcttg cggataatct
     3361 tttccagcag atggcccggg cttttcgtgc ggtaacgata ggaatggatt cccgcccgtt
     3421 cgatatcata gaggtaatca tttacaaact cctttccgat tccccttaat ttcccctcaa
     3481 tggacaaata gtcatcataa atagcagcca gctcctccca tgggacattg gctgccttaa
     3541 aatccgcctc atcaatatta taagctctta aaaatgcgtt ccgttcgaac acgttttcac
     3601 ttccttccag gctaaatcaa atttacgttt catttgaaat gctcatgctc tgtactgcaa
     3661 aatccgtttc tatgatactg caaacaaagc gtattttgca aggaaaaaat tcacttctct
     3721 attcatgcag ctccagagga agcccgtcag gatctgcaaa aaatgtcatt ttctttcccg
     3781 taaactcatc cagccggatg gattccacaa aaatcccctt ttccttcagc tctttcacat
     3841 ctttctctat atcctccgta taaaaggcca gatggcgcag tccggctgcc tccggtctgg
     3901 tcactcgttt gggaggattt gatactccaa aaatttccag ttctgtagtg tcattgatcc
     3961 gaagatccag cttccagtct cctctgtccg gccggtaatt ctcccgtatc acggaaaatc
     4021 ccagcttatt tacataaaaa tctcttgatt tttcataatc ggaaacaatg atcgcaatgt
     4081 gatgtatggt cgaaaatttc atatatctcc tctctccata taacaaattc ctatccaata
     4141 ttatctacca tcagcatatt cagtttctgc cactgctgca gttcccttct tgtgatcaaa
     4201 aggcgctttt taaactcctc tgtgtgctcc tgaggatgca gcgtcaccag gcatttaaac
     4261 gcgcagggca taagatctgc actggaaatc ccgcacagaa ggccgtccgt tgaatcgcgc
     4321 agctctaagg ggttaaaaat ataaagcaga tcctcctcca gaggattata gcggtttaca
     4381 aacgaaaaac ggatcagcat atcactgtag atcacctttc cggtgtaata cagcttgctg
     4441 ctgcgcccgg agggattgac agcactcacc gacagatctg cctcataact ccccgcctct
     4501 ttcgtttccc ggatatcgat gatcccgtcc ttcagccgtt tataacggcc gtcataaaaa
     4561 tagaaataca gccgtctcgc ctgaaaaaac gggctgatcc cgctgtttgc ggaagggagc
     4621 ctggccccct cctcttcctg aaccggaaga tccaccagct gccccggctg tacctccaga
     4681 actctggcaa tctcggtcag cgtgacaata tccagagaaa cctctcccgt ctcatactta
     4741 ctcacactgg cccggctctt atggatcctg tcagccagct gctgcagcgt catctgtctg
     4801 ttttttctgt atctgcggat ctgtgctccc acgtacagat tcacctgctc cacccttttt
     4861 tcctccccat ctgtatttat cctcccttct cccggtaaat agggaaatgt ttcatataaa
     4921 tatacatttt acattttatc caactatatt tccgctatta tacctctttt tccggtattt
     4981 ttctagttat attcaatttt tatattatat tttctcattt aacgaaactt ttcggcggtt
     5041 gctttttctc tgcaaaccga tataatcata ctgtttgggt caaaacagat ttggcccctt
     5101 ttacaatcca acaagacaac gaggtattca aaacatggaa gcaaagaaaa aaagcagctt
     5161 tacaggctcc ttaggctttg tgctggcagc agcaggaagc gcagtaggag taggaaatat
     5221 ctggcggttc ccctatctgg ccgccaagga cggaggcgga ctattcctga tcatctatct
     5281 ggcgctggta cttaccttcg gctttaccct tcttgtcaca gatgtggcca tcggacggcg
     5341 caccaagaca aacgcccttc acgcctttgg gaaaatgcag aaaaaatgga gctttttagg
     5401 ttatcttacc ttctgcgttc cggccattat catgacctat tactccgtca tcggcggatg
     5461 gattttaaag tatctggctg tctatcttac cggagcggga aaggcagccg cccaggacgg
     5521 atattttaca gactttatca cctcacccgt atcacctgtg gttttcatgc tgatcttttt
     5581 atgttttacc gcactggtcg tataccgcgg ggtagaaaag ggcattgaaa aattttcccg
     5641 gatcgtcatg ccgggactgc tcttaatgat tatcgggatc ggcctttttt ccctcacatt
     5701 aagcagtacc ggagcagacg gaacagagcg gaccggcctt cagggactga cagtctacat
     5761 tatgccgaat cttgacggag ttactcctgc cagatttttg gaaatccttc tggatgccat
     5821 gagccagctg ttcttttcct taagtgtttc tatgggaatc atgatcacct acggctccta
     5881 tgtaaaaaag gatgtaaacc taagcaaatc cttaagccag attgagattt tcgatacaac
     5941 ggttgcattt ctggccggac ttatgatcat ccctgcggtc tatgtatttt tcggcacaga
     6001 aggaatggcc tccggtccca gcctgatgtt cgtatccctt cccaaggtat ttgaagccat
     6061 gggacccctg ggcggagtcc ttggaatgat atttttcctt atggttgtgt ttgcggctct
     6121 tacctcctct gtatccattc tggaaacact ggttgcaaac tgtatggaga ttttccactc
     6181 cacacgaaaa aaaaccagcc ttgtgatcgc actactctct gcggcagcct ccgtgatcat
     6241 ctgtatgggc tataacgtat tctactttga aatgaagctg ccaaacggag catccgccca
     6301 gcttctggat ctgatggact atatcagcaa cagctttctc atgccattca tttccctcct
     6361 cacctgcatc tttgtaggct ggatcatcag gccgacgtgg atctgcgagg aaatggaagc
     6421 cagcggacat acctttaagc gcaaaaaaat gtatgccttt atgatccggt atgtgactcc
     6481 gatcatcatg gctatgctgt ttttacagtc tgccggtgta tttgaaataa taaaaaacat
     6541 gtttggctga ttcatgccat cagcaaaaaa gtcccttccg ctctgtcctg tgacgaaaac
     6601 cggaagggac ttttttattt tctgtcaaat acgtttttat accgttccac tgtaaatatt
     6661 ttccagtttc tcatctgtaa gctgtacatg gtagaacaga tcaaagaatt cctttacttc
     6721 ttcatccaca ttgtaattca gatactccgg ataaatgagg ccggacagcc agcggattcc
     6781 cacaatacgg tttggcccca taggacggtc catccagcta aagggcgtat ttggcgtacc
     6841 gtatacctgc tgatcctgca ctgcctttaa agatgcgtaa tccggattgg ccagaattgc
     6901 ctcagcagcc gaagcgccgc tcgtatccgc ctttggctct ccgtttacca caattacatc
     6961 cggatcccag gcaagaagct gctcggcaga aatctgcact ctggagcctt ctcccatttc
     7021 cagatctgcc acattgactg catttaccat atcgatgatc tgtccgtgag cagagccggc
     7081 cggagcggtc tccagggaat cctctccgtt tccataataa atacgtacct ttttgtcctc
     7141 aggaacctcc attttttcaa tatctgcaaa ggtcttttcc gcataggaag cgagcttttc
     7201 ggcctgttcc tcctccccca gcagttctcc cataaagcgg taagcttctg cggaagccga
     7261 cagatctccg tccacagcca caacaggaac tcccagactc tttgacagct tatcgcagtc
     7321 tgatatcatc ttatcattga tggttcccac attgactgca atggtggggt ctgctgcaat
     7381 aaccgcctca taattgatgg aatcgcccat tccaaaatta ggaagatcgt gatattcttc
     7441 caaaataatg ctcttttcca gctcattcag ctcataattc cagccaagaa gcttatccgg
     7501 agccagcgta tacataaaaa tagccgttac agtactggaa gagaataccg tttcaatttc
     7561 atccggcacc acaaccgtgc ggcctgccat atccgttatc tcccgctctc ccgccgcatc
     7621 ggctgcctca gatgttttct ctgcctgagt gctctgttca gctgttttcg tttcagcttg
     7681 gctctgcatg ctttctttag ccgtttctgt ttttgcagaa gaagcagaac atcctgtaat
     7741 gcttcctgca gccagggtaa gcgccatcac tgccggtaaa atcttttttc ctcgtcccat
     7801 ttttcctctc ctcttaattg acattctgat ctttatgcgt tatattttta aatatcgtac
     7861 ttttttataa aatataacgg tattttcata ctagcacggc aaaatttttt tgtcaagcat
     7921 caggaaaggc ggaaacgtcc atgttgaatc atcatttcga agtaaagcag gaagcggccc
     7981 agagaaggcg gtatggcctt cttatcaccg gactgcttat ctttctattt accgcagtcc
     8041 ttttatcctt ctgggtgggc tactatcccc tgtccccctc tcaggtcgta acggcatttt
     8101 tatcccggtt cgggtatgag ggagaaatcc ttccccaggc cgttaccatt ttctggaata
     8161 ttcgtcttcc ccggatcctg tccgcagtct ttatcggagc ctcgctgtcc acagcgggcg
     8221 cagcctatca gggaatgttc cgcaatcctt tagtctcccc cgacatcttg ggagtctcct
     8281 ccggagccag tctgggagcc gcttttgcaa tccttttcgg cgcctctaac tggcttatac
     8341 agctttccgc ctttgcaggc ggcaccgcag cggtcgctgc ctcttatctg atcagccgca
     8401 gatccgtcca ttccataacc ctcagcctgg ttctcaccgg ctccatgatc atggcgctgt
     8461 gcaatgcagg tgtaaccatg atcaaatatg tggcagatcc caatgatgtg cttcagcaga
     8521 tcaccttctg gctcatgggg agccttacaa aaaccgatac ccgctccttt ttatggagcc
     8581 tgatccccat gtctgcggga ctggtcatca tctttcttct gcgctggcgc atcaatctcc
     8641 tcactctgga tgaagaggaa gccaaaagcc ttgggatcaa tatccgtctt taccgtctga
     8701 tcctcattgc tgcttctacg ctcctcagcg ctgcctccgt ttgtctgggc ggcctcatcg
     8761 gctgggtcgg actgatgatc ccccatatgg cacgggcttt ggtcggagcc gactacggcc
     8821 gtctgattcc tgccagcgcc atgctgggag ccacctatct ggttctcatg gatgatctgg
     8881 cccgttccct tctttccatg gaactgcccc tgggcgttgt aacttctatt atgggagcgc
     8941 ccttttttgt atctcttatc attcagaaaa aggagtaaac gaccatgctt ttacaactgc
     9001 agaatgtttc cggcggatat gggaaaggcg atattgtaaa aaatgtaagc tgcgaagcag
     9061 acgaaggaga cattctctgt ctggccggcc ccaatggctg cggaaaaacc actctgttcc
     9121 gtctgataat ggggtcaatc cctctttccg gaggccgtat cctcatagac ggacaggatc
     9181 atttaaagct ttcccccaga gagcttgcaa accgtatcgc ctatattcca cagtaccatt
     9241 c
//
LOCUS       DBMDUH010000110         9177 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_3521_length_9177_cov_5.248568, whole genome shotgun sequence.
ACCESSION   DBMDUH010000110 DBMDUH010000000
VERSION     DBMDUH010000110.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 9177)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9177)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9177
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3521_length_9177_cov_5.248568"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            141..443
                     /locus_tag="ACZPCG_09540"
     CDS             141..443
                     /locus_tag="ACZPCG_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1285333.1"
                     /translation="MLYDRVKQLCSNEGITITELERTLKFGNGTIHNWNKSEPSVAKA
                     MAVAQHFGVGLDALVSNTKLPSKESREIFARFDGYTEDQKNLIKCYMSLIETGKVR"
     assembly_gap    459..468
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <469..1216
                     /locus_tag="ACZPCG_09545"
     CDS             <469..1216
                     /locus_tag="ACZPCG_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009268536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HIR1285334.1"
                     /translation="MVSARDLHEGLEIGTRFNDWFPRMMEYGFSEGKDFYSKMSKTSE
                     SGGRPSVDYQISVDMAKQICMIQRSEKGRQYRQYFLDLEKAWNTPEQIMARALRFAQQ
                     SMDNLKEHCQFLGAQVVEQQKVIEELQPKAFYYDMILQCKDLIATTVIAKDYGMSAKK
                     FNVMLHDMGIQFKQGDTWVLYAKYQGNGYLKAKTCNYADTEGVQHSREHSYWTQKGRL
                     FLYDFLKQQGVLPVVDMQPYKAQRSISQRV"
     gene            1236..1760
                     /locus_tag="ACZPCG_09550"
     CDS             1236..1760
                     /locus_tag="ACZPCG_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006772847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HIR1285335.1"
                     /translation="MHDLTRTTITSMEAAEWCGKKHNELLKDIRRYISQLGEGKIPHT
                     DFFKESTYVTEQNKALPCFLVTKKGCEFIAHKMTGQKGTEFTARYINRFHEMEHKKLP
                     CPLNPQIASDVADLGRVTERVMKNQGSAPHKIAEAFKMISEQFGIQLPDDFVKIPEYE
                     QMSMTELLTGSGKD"
     gene            1778..2017
                     /locus_tag="ACZPCG_09555"
     CDS             1778..2017
                     /locus_tag="ACZPCG_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1285336.1"
                     /translation="MTAREYMAYSRQRSDNIKAIIAEHKQRKGMTDAMVAKAAGINPG
                     TFVQRKRNPGTFRLNDLWAICNVLDVPEEQRNTIL"
     gene            2027..2248
                     /locus_tag="ACZPCG_09560"
     CDS             2027..2248
                     /locus_tag="ACZPCG_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285337.1"
                     /translation="MQSEAIKYQPPVEGVVVTQEEEQQILKLGRIMREQLDQSHKDVR
                     LFGRLSTVMAFLLGMETVLLLVAAGIIAL"
     gene            2441..3712
                     /locus_tag="ACZPCG_09565"
     CDS             2441..3712
                     /locus_tag="ACZPCG_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR1285338.1"
                     /translation="MSMKINKLEIENVKRVKAVKIEPTANGLTIIGGKNNQGKTSVLD
                     AIAWALGGDRYRPSQAQREGSVISPNLHIVMNNGLIVERRGKNSDLKVTDPNGKKAGQ
                     QLLNEFVEQLALDLPKFMEATSREKAQTLLQIIGVGPRLADLERQEKELYNERTYIGR
                     TADQKEKYAKEQPYYPEVPSVPVSASELIRQQQEILAQNGENQRKRERRHQLEQEYQS
                     VTEQIQALLAKQGQLEADLKIARETSEGLTDRSTAELEENISNIEEINRKVRANLDKD
                     KAEEDAKGYREQYKRLTAQIEEIRSQKTDLLKEADLPLPGLGVEDGELVYHGQKWDNM
                     SGSEQLKVSTAIVRRLNPECGFVLLDKLEQMDLDTLHEFGQWLEQEGLQAIATRVSTG
                     GECSLIIEDGYVAGQEHPATEEKKEWKAGVF"
     gene            3713..4840
                     /locus_tag="ACZPCG_09570"
     CDS             3713..4840
                     /locus_tag="ACZPCG_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1285339.1"
                     /translation="MEIIRGKLPGAKKVVVYGPEGIGKSTFASRFPDPLFIDTEGSTK
                     DMDVARTEAPSSWMMLMEQVMYVRKHPELCRTLVIDTADWAEMLCVTQLCDKNQKNSI
                     EEFGYGKGYVYLQEEFGRFLNLLEEVVKAGVHVVLNAHAKMRKFEQPDELGSYDRWEM
                     KLSKNVAPMVKEWADMVLFANYKTFVVNVDGQGAQKGKNKAQGGKRVMYTAHHCCWDA
                     KNRYGLPEELPFEYAGIRHIIEPSGAPEKVETPPVKEAAPPIPPVQAPPKTADPPVSN
                     KSHEEPEKEEKAAPPVRNEGVNLDPRIPKKLRDLMAANDVCEWDMENVVFAKGYVTEG
                     TPIWEFETQNPGIIDGLLVASWDQVYAAIKEMKEKDALVFN"
     gene            4855..5307
                     /locus_tag="ACZPCG_09575"
     CDS             4855..5307
                     /locus_tag="ACZPCG_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009215763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HIR1285340.1"
                     /translation="MMADLGQEIGWDDEIENEGADFEPLPEGIYEFEVRTMQRGRFPG
                     SDKMCACNTAELTLVIVDNDGKEHQVFESLKLNSKMEWLLSQFFLCIGQKKKGEALRP
                     NWNAVPGSRGMAEITVNEYKDKNGNLKKNNRVSKYLAPEPKQFKAGVF"
     gene            5307..6896
                     /locus_tag="ACZPCG_09580"
     CDS             5307..6896
                     /locus_tag="ACZPCG_09580"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR1285341.1"
                     /translation="MKLRPYQEEARESIFEEWDKGVQKTLLVLPTGCGKTVVFAKVAE
                     DCVRRGDRVLILAHRGELLEQAADKIGKATGLGCATEKAEQSCLGSWFRITVGSVQTL
                     MREKRLGQFPAGYFNTIIIDEAHHCISESYQKILQHFDSAHVLGVTATPDRGDMQNLG
                     QVFDSLAYEYTLPKAIREGYLSPIKALTIPLQLDLSGVSMQSGDFKAGDIATALDPYL
                     YQIADEMEKYCKDRKTVVFLPLVKTSQKFKEVLNEKGFRAAEVNGESKNRAEVLEAFD
                     RGDYNVLCNSMLLTEGWDCPSVDCVIVLRPTKVRSLYSQMVGRGTRLCEGKDHLLLLD
                     FLWHTERHELCHPADLICEKKEVARKMTENLEESAGCPVDLEEAEKRASEDVIAEREE
                     SLAKQLAEMKNRKKKLVDPLQFEMSIQAEDLAGYVPAFGWEMAPPSDSQKRELEKRGI
                     LPDEIDNAGKAGKILDRLHKRQEEGLTTPKQIRCLEKYGFRHVGTWSFEAGKHMIDRI
                     AAMGWRGAPPGVDPGTYIPEK"
     gene            6910..9117
                     /locus_tag="ACZPCG_09585"
     CDS             6910..9117
                     /locus_tag="ACZPCG_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006862371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR1285342.1"
                     /translation="MEHNQYDLLEVLDHIEPAELDYQQWVNVGMALEIEGYGVDVWDA
                     WSRRDPGRYHPGECRKKWAGFHGAGSPVTGGTLVQYAREQGWTPPCDPGVALGWDDTI
                     SAEGVVVDSNWVEDKDVQEPLRWNPADELIRYLEALFEAGENVGYVVKSWQKDEKWLP
                     ADKGAYDRTAGELIEALSACGGDIGKVLGDYNSEAGAWIRFNPLDGKGVRNENVTEYR
                     YALVESDSMEIGKQHALIRELELPVACLVHSGRKSLHAIVRIDAADYGEYRRRVDYLY
                     DICRRNGLEIDQQNRNPSRLSRMPGVMRGSQKQFLVDTNIGKGSWEEWKEWIESVNDD
                     LPDPEGLGDVWEQMPELAPCLIEGVLRQGHKMLIAGPSKAGKSFLLIELCIAIAEGRK
                     WLSWQCAQGRVLYVNLELDRASCLHRFKDVYQAMGLPPEHLENIDIWNLRGKSRPMDK
                     LAPMLIRRAAKKNYIAVIIDPIYKVITGDENSADQMANFCNQFDKVCTELKVATIYCH
                     HHSKGSQGGKKSMDRASGSGVFARDPDAMLDMIELELSEDVLKAEENKAVCAACKQYL
                     DAHFKWEDDLSEDDLCSAYQMMNYCENLLDKWQWLNLQRIVEEAKKRARGLTAWRIEG
                     TLREFPKFPAVNAWFSYPVHQMDTVGVLGDIQPEAELPPWKRAMDKRKPKEQKAKERK
                     ESIATAYDACTTDGKVTVSDLAEYLGVTEKTIRNRLKEHGGFVIDDSEVRKKT"
BASE COUNT         2594 a         1975 c         2802 g         1796 t
ORIGIN      
        1 gctagtaaat cataataaca caatttacgt agtggttaat atgaaaatta tctattgaaa
       61 tatcacaata ctataataga ttatattcta gcatttgtca acggtttaat aagatttttt
      121 ataaagcagg aggtgaaaaa atgttatatg accgcgtgaa acagctctgc tcgaatgaag
      181 gtattacaat cacggaatta gagcgtactc taaaatttgg taatggaact attcataact
      241 ggaataagtc agaaccgtcg gtcgcaaaag cgatggcggt ggcgcaacat ttcggtgttg
      301 gtctggacgc tcttgtttct aatacaaagc ttccgtcaaa ggaatccaga gaaatatttg
      361 ctcgttttga cgggtacacc gaagaccaga agaaccttat taaatgttat atgtcattga
      421 ttgagacggg aaaggtaaga taatgaacta tttaatcann nnnnnnnnca tggtatcagc
      481 gagagatttg catgagggac tggaaatcgg aacaaggttc aatgattggt ttccgagaat
      541 gatggagtat ggattttcag aaggaaagga tttttactca aaaatgagta aaacctctga
      601 aagcggtgga aggccatctg ttgactatca gatttccgta gacatggcaa agcagatttg
      661 catgatacag cgttcagaaa agggccgtca gtatcgtcag tattttcttg atcttgaaaa
      721 agcgtggaat acaccagagc agattatggc aagagcattg aggtttgccc agcaatctat
      781 ggataatctg aaagagcatt gccagttttt aggtgctcag gttgtagagc agcagaaagt
      841 tattgaggag cttcaaccca aagccttcta ttatgacatg atacttcagt gtaaggattt
      901 gattgcaacc acagttattg caaaagatta cggaatgtcg gccaagaaat tcaatgtaat
      961 gcttcacgat atggggattc agttcaaaca gggtgatacc tgggtattat atgcgaagta
     1021 tcaaggtaat ggatatttga aggctaaaac gtgtaattat gcagatacgg aaggcgttca
     1081 acattctcgt gaacattctt attggactca gaagggcaga ttatttttat atgatttttt
     1141 gaaacagcaa ggagttctgc cagtagtaga tatgcagcca tacaaagcac agaggagtat
     1201 ttcgcaaaga gtttagaaga aggagggtac ataacatgca tgatttaaca agaaccacca
     1261 tcacatcaat ggaagccgcc gagtggtgcg gaaagaagca taatgaactg ctgaaagata
     1321 ttcgtagata tatcagccag ttaggagagg ggaaaattcc ccacaccgat tttttcaagg
     1381 aatctacata cgttaccgag caaaacaagg cgctcccctg ttttcttgtc accaagaaag
     1441 gctgtgagtt catcgcccat aagatgaccg gacagaaggg aacggagttt accgcaaggt
     1501 atattaaccg attccatgag atggagcata aaaagctccc ctgcccgctc aacccgcaga
     1561 ttgcttccga tgtggcagat ctgggccgag taacggaacg ggtcatgaaa aatcagggtt
     1621 cagcgccgca taaaatagcg gaggctttca aaatgatttc tgaacagttt ggaatccagc
     1681 ttccagatga tttcgtgaag attccggagt atgagcagat gagtatgacg gagcttttga
     1741 caggctctgg caaagattaa gagagggggt atcccacatg acagcaaggg agtacatggc
     1801 ctatagcagg cagcggagtg ataacattaa ggccattatc gcagagcata aacagcgtaa
     1861 aggcatgacc gatgcgatgg tagccaaggc ggcaggcatc aatccgggga cattcgtcca
     1921 gcgaaaacgg aatcccggaa cgttccggct gaatgatctc tgggcgatct gcaacgtgct
     1981 ggacgtgccg gaggaacaga gaaatacgat cttataggag ggaatcatgc aatcagaagc
     2041 aattaagtac cagcccccag tggagggtgt tgtagtcacc caggaagagg agcagcagat
     2101 cctgaagctg ggccggatca tgagggaaca gctggatcag agtcataagg atgtgcggct
     2161 gtttggcaga ctgtctacgg tgatggcgtt cctgctgggg atggagacgg tgctgctgtt
     2221 ggtagctgcc ggaataatag cactgtgaga ggaggtgagg acgatggata aggatgcttg
     2281 cggcacttgc cgtaataaga atcggtgcat agaacggagc aggatgtatc cgtgcctgag
     2341 ttacaagaag aaaaaaggac ctgcacagcg gcaactgtgg tagatccaga aactaaaata
     2401 atccaccctt attataaggg gaaatttgga ggaatgcaag atgtcaatga agatcaataa
     2461 gctggagatc gagaacgtaa aacgtgtgaa ggcggtgaag atcgagccga ccgccaacgg
     2521 ccttacgatc atcggaggga agaacaacca ggggaagacc tctgtcctgg atgcgatcgc
     2581 ctgggccctg ggaggggacc ggtaccgtcc gtcccaggca cagagggaag gctccgtaat
     2641 ctcgcccaac ttacatattg ttatgaataa cggcctgatc gtggagcgcc ggggaaagaa
     2701 cagcgacctg aaggtgacgg atccgaacgg gaagaaagcc ggccagcagc tcctgaatga
     2761 gtttgtggaa cagctggccc tggaccttcc caagttcatg gaagcgacca gccgggaaaa
     2821 ggcccagacc cttttgcaga tcatcggtgt gggcccaagg ctggcagacc ttgaacggca
     2881 ggaaaaggag ctgtataacg aacggaccta tatagggcgc acggcagacc agaaagagaa
     2941 gtacgcaaag gagcagccct attatccgga agtcccgtcc gtccctgtat ccgcttccga
     3001 actgatccgc cagcagcagg agatcctggc acagaacggg gagaaccaga ggaagaggga
     3061 gcggcgccac cagctggagc aggaatacca gtccgttacg gaacagatcc aggcgctcct
     3121 tgcaaagcag gggcagctgg aggcagacct taagattgca agggagacct cagaagggct
     3181 tacggatcgg tctaccgcag agctggagga gaatatctcc aatatcgagg agatcaaccg
     3241 taaggtgcgt gcaaacctgg acaaggataa ggcggaagag gatgccaaag gataccggga
     3301 gcagtataaa cggctgactg cccagatcga ggagatccgg agccagaaga cagacctgtt
     3361 aaaagaggcg gacctgcccc ttccaggcct gggcgtggaa gacggggaac tggtctacca
     3421 cggacagaaa tgggacaata tgtccggctc cgaacagctg aaggtatcca ctgccattgt
     3481 gcgcaggctg aacccggaat gtgggtttgt gctgctggat aagctggagc agatggatct
     3541 ggataccctg catgagttcg ggcagtggct ggagcaggaa gggctgcagg ccattgcaac
     3601 ccgcgtatcg acgggagggg agtgcagctt gatcattgag gacggatatg tagccggaca
     3661 ggaacatccg gcaacagaag aaaagaagga atggaaggca ggtgtatttt aaatggagat
     3721 cattagagga aaactgccgg gggcaaaaaa ggtagtggtt tacgggccgg agggcatcgg
     3781 gaagagtacc tttgcttccc ggttcccgga tccgctgttt attgatacag agggaagcac
     3841 caaggacatg gatgtagccc ggacggaagc acccagcagc tggatgatgc tgatggagca
     3901 ggtgatgtat gtaaggaagc acccggagct ctgccgcaca ttggtcattg acacggcgga
     3961 ctgggcggag atgctctgcg ttacccagct gtgtgacaag aaccagaaga acagcattga
     4021 ggagtttggt tacggaaagg ggtatgtata tctccaggaa gagttcgggc ggttcctgaa
     4081 cctgttggaa gaggtggtaa aggcaggcgt ccatgtggtt ttgaacgccc atgctaaaat
     4141 gcgcaaattc gagcagccgg atgagctggg ctcttatgac cgctgggaga tgaagctgag
     4201 caaaaacgta gcccccatgg tgaaggaatg ggcggatatg gttctgtttg ccaactataa
     4261 aacctttgtg gtgaacgtgg acggccaggg agcccaaaaa gggaagaaca aggcccaggg
     4321 cggaaaacgg gtcatgtata cggcccacca ctgctgctgg gatgcaaaga accggtacgg
     4381 gctgccggaa gaactgccct ttgagtatgc agggatccgg catatcatag aaccctccgg
     4441 ggcaccggaa aaggtagaaa cacctcctgt taaagaggca gctccgccaa tcccgccagt
     4501 acaggcaccg cctaaaacag cagaccctcc ggtcagtaac aagagccatg aggagcctga
     4561 aaaggaagag aaagcagcgc caccagttag gaatgagggc gtgaatctgg acccgaggat
     4621 acccaagaag ctgagggatc tgatggcagc aaatgacgtg tgtgagtggg atatggaaaa
     4681 cgtagtattt gcaaagggtt atgtcaccga agggacgccc atctgggaat ttgaaaccca
     4741 gaacccggga atcattgatg ggcttctggt tgcatcctgg gatcaggtat atgcagcgat
     4801 taaggaaatg aaggaaaaag acgcattggt atttaattaa acaggaggat caagatgatg
     4861 gcagatttag gacaggaaat tggatgggat gacgagattg aaaacgaggg tgccgatttt
     4921 gagccgttac cggaaggcat ctatgaattt gaggtacgca cgatgcaaag agggagattc
     4981 cctggaagcg acaagatgtg cgcctgcaat actgcggaac tgacattagt gattgtagat
     5041 aatgacggca aggagcatca ggtttttgag agcttaaagc tcaacagcaa gatggagtgg
     5101 ctgttaagcc agttcttctt atgcatcggc cagaaaaaga agggggaagc gctgcgtcca
     5161 aactggaacg ccgtccctgg atcccgtgga atggcagaga tcacggtcaa tgaatataag
     5221 gataaaaacg gaaacctaaa gaagaataac cgggtaagca agtatcttgc cccggaaccg
     5281 aagcagttta aggcaggtgt attctgatga aattaagacc ttaccaggaa gaagcaaggg
     5341 aatccatttt tgaggagtgg gataagggcg tccaaaagac gctcctggtc ctccctactg
     5401 gctgcggaaa gactgtggtg tttgcgaaag tggcggagga ctgtgtgcgc cgcggggacc
     5461 gggtgctgat cctggcccac cggggggagc tgttggaaca ggcagcggat aagatcggga
     5521 aagcgaccgg tttgggatgt gcaacagaaa aagcggagca gtcctgtctg gggagctggt
     5581 tccggatcac cgtcgggtct gtccagaccc ttatgaggga gaaacggctg ggacagttcc
     5641 cggccggtta ttttaacacg atcatcattg acgaggccca ccactgcatt tcagagagct
     5701 accagaagat cctgcagcat tttgattcgg cccatgtgct gggcgtgaca gccacaccgg
     5761 accgcgggga catgcagaac cttggacagg tatttgacag cctggcttac gaatataccc
     5821 tgccaaaggc aatccgggaa ggatatttat ccccgattaa ggccctgaca atcccgctgc
     5881 agctggattt gtccggcgta tccatgcagt ccggggactt taaggcaggc gatattgcaa
     5941 ccgccttgga cccgtacctg taccagatcg cggatgagat ggagaaatac tgtaaagacc
     6001 gtaaaacagt ggtgttcctc ccgcttgtaa agaccagcca gaagtttaag gaggtcctga
     6061 acgaaaaggg cttccgtgca gcggaggtaa acggggaaag caagaaccgg gcggaagtgc
     6121 tggaagcctt tgaccggggg gactataacg tcttgtgcaa ctccatgcta ctgacggaag
     6181 gatgggactg cccctctgtt gactgtgtga tcgtactgcg ccctacgaag gtgcggagcc
     6241 tgtacagcca gatggtaggg cgggggaccc gtctctgcga agggaaggac catctccttc
     6301 tgttggactt cctgtggcat acggagcgcc atgagctgtg ccacccggca gacttgatct
     6361 gcgagaagaa ggaagtggcc cggaagatga ccgaaaacct ggaggaatcc gccggctgtc
     6421 cggtggacct ggaggaggcg gagaaacggg cttccgagga tgtgatcgca gagcgtgagg
     6481 aatcccttgc caagcagctg gcggagatga agaaccgtaa aaagaagctg gtggatccat
     6541 tgcagtttga aatgagcatc caggcggaag acctggccgg gtatgtacct gccttcgggt
     6601 gggagatggc cccgccgtcg gacagccaga aacgggagct ggaaaaacgg gggatccttc
     6661 ccgatgagat cgacaacgca gggaaggcag gcaagatcct ggatcgtctc cataagaggc
     6721 aggaagaagg gctcacaacc ccgaaacaga tccggtgcct ggaaaaatac ggattccgcc
     6781 atgtaggcac ctggagcttt gaagcaggaa agcacatgat cgaccggatc gcggcaatgg
     6841 gatggagagg ggcgcctccg ggtgtggacc ctgggacgta tataccagag aaataaggag
     6901 cagtaagaca tggaacataa ccagtatgac ctgttggagg tcttggatca tatagaaccg
     6961 gcagaactgg attaccagca gtgggtgaac gtggggatgg ccctggagat cgaggggtac
     7021 ggcgtggacg tctgggatgc ctggagccgc agggaccccg gcaggtacca tcctggggaa
     7081 tgccggaaga aatgggcggg gttccatggg gcaggatccc cggtgactgg agggaccctt
     7141 gtacagtatg caagggaaca gggatggacg cctccctgcg acccgggcgt ggcccttgga
     7201 tgggatgata ccatatcagc cgaaggcgtg gtggtggaca gcaactgggt agaagacaag
     7261 gacgtacagg agccattaag atggaacccg gcggacgagc tgatccgtta cctggaggcc
     7321 ctgtttgagg caggggaaaa cgtagggtat gtggtaaaga gctggcagaa ggatgagaaa
     7381 tggctgccgg ctgataaagg tgcatatgac cgtacggcag gggagctgat cgaggcgctt
     7441 tctgcctgcg gcggggacat agggaaagtc ctgggggact ataacagcga ggcgggcgca
     7501 tggatccgct tcaaccccct ggacgggaaa ggcgtccgga atgaaaacgt gactgaatac
     7561 cgttatgcct tagtagaatc ggacagcatg gagatcggca agcagcacgc cctgatccga
     7621 gagctggaac ttccggtggc ctgtctggtc cattccggca ggaagagcct ccatgccatt
     7681 gtacggatcg atgcggcgga ttacggggag taccgcaggc gggtggatta cctgtatgac
     7741 atctgccgca ggaacggcct ggagatcgac cagcagaacc gtaacccgtc ccgtctgtcc
     7801 cggatgcctg gtgtcatgcg cgggagccag aaacagtttt tggtagatac caacatcgga
     7861 aaagggagct gggaagagtg gaaggaatgg atcgaaagcg tcaatgacga cctgccggac
     7921 ccggaaggcc ttggggatgt gtgggagcag atgccggaat tggccccgtg cctgatcgaa
     7981 ggggtgctcc gccagggaca caagatgctg atcgcaggcc cttccaaagc cggtaaatcc
     8041 ttcctgctga tcgaactgtg cattgccatt gcagaaggcc gcaaatggct ctcctggcag
     8101 tgtgcacagg ggcgcgtact gtatgtaaac ctggagctgg atcgtgccag ctgcctgcac
     8161 cgtttcaagg acgtatacca ggccatgggg ttgccgccgg agcatctgga aaacattgac
     8221 atatggaacc tgaggggaaa gtcaaggccc atggacaagc tggccccgat gctgatccgg
     8281 agggcggcaa agaagaatta cattgcagtt atcattgacc cgatctataa ggtcatcacc
     8341 ggggacgaga acagtgcgga ccagatggcg aacttctgca accagtttga caaggtgtgt
     8401 acggaactta aggttgctac catttactgc caccaccaca gcaagggcag tcagggcgga
     8461 aagaagtcca tggaccgtgc atccggttcc ggagtgttcg cccgcgaccc ggatgccatg
     8521 ctcgacatga tcgagctgga gctatcagag gatgtcttaa aggcggagga aaacaaggcg
     8581 gtctgtgcag cctgcaaaca gtatctggac gcccatttta agtgggagga tgacctttca
     8641 gaggatgacc tgtgcagcgc ctaccagatg atgaattact gcgagaacct gctggataag
     8701 tggcagtggc tgaacctgca gcgcatcgtg gaagaggcaa agaagcgggc cagggggctg
     8761 acggcgtggc gaatcgaggg gacactgagg gagtttccaa agttcccggc agtcaatgcc
     8821 tggttcagct atccggtcca tcagatggac acggtagggg tgctggggga catccagccg
     8881 gaggcagaac tcccaccatg gaaacgtgct atggacaaac gaaagcccaa agagcagaag
     8941 gcaaaagagc ggaaagaatc cattgcaacg gcttatgatg cctgcacaac agatggaaaa
     9001 gtgaccgttt ctgacctggc tgagtacctg ggagtgacag aaaaaacgat ccggaaccga
     9061 ttaaaagagc acggaggatt cgtgatagat gatagtgaag taaggaaaaa aacgtaaaaa
     9121 ttcccttttc actagggaag aaaaaaacat aattgacgtt attaaaaaaa aaaaaaa
//
LOCUS       DBMDUH010000111         9174 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_3522_length_9174_cov_9.851207, whole genome shotgun sequence.
ACCESSION   DBMDUH010000111 DBMDUH010000000
VERSION     DBMDUH010000111.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 9174)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9174)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9174
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3522_length_9174_cov_9.851207"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /gene="fabF"
                     /locus_tag="ACZPCG_09590"
     CDS             <1..1038
                     /gene="fabF"
                     /locus_tag="ACZPCG_09590"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008726614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HIR1285343.1"
                     /translation="RRMEPFSQFAVAAAKQALDQSGIDMEKEDPFRVGVSVGSGIGSL
                     QAMERSEKKLLEKGPSRVEPLLVPLMICNMAAGNVAIQFGLKGKCINVVTACATGTHS
                     IGEAFRAIQYGEADVMVAGGTEASITPIGIAGFTSLTALNTTDDPKRASIPFDKDRNG
                     FVMGEGAGVVVLESLEHAKARGANILAEVVGYGATCDAYHITSPAEDGSGAAKAMEFA
                     MKDAEIMPEQVDYINAHGTSTHHNDLFETKAIRLALGDQAEKVKINSTKSMIGHLLGA
                     AGGVEFITCVKSIQDGYVHATLGLEEPGEGCDLDYTQGDGVEMNVNYAISNSLGFGGH
                     NASLIVKKFTE"
     gene            1058..1588
                     /gene="accB"
                     /locus_tag="ACZPCG_09595"
     CDS             1058..1588
                     /gene="accB"
                     /locus_tag="ACZPCG_09595"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HIR1285344.1"
                     /translation="MNIDEILTLVQAVSDSSLTSFKLEQGDTVISLRKEKEEAKIVTV
                     GAPAAPAVFAASAPAHVLPAPETVAASGVQVPAGVPAGQTAAPAEAKDAGTDIGSDKV
                     ITSPLVGTFYSSSSPDAEPYVKAGDTVKKGQILGIIEAMKLMNEIESEYDGVVEAVLV
                     NNEDVVEYGQPLFRIK"
     gene            1613..2038
                     /gene="fabZ"
                     /locus_tag="ACZPCG_09600"
     CDS             1613..2038
                     /gene="fabZ"
                     /locus_tag="ACZPCG_09600"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008726610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HIR1285345.1"
                     /translation="MLMGIKEIQEIIPHRHPFLLVDCIEELEPGVRAVGYKAITYDES
                     FFRGHFPQEPVMPGVLMVEALAQTGAVAILSQEGFKGKTAYFGGIDKLKFKHKVVPGD
                     RLRLECEIIRQKGPVGIGKATATVDGKVAVTGELTFMIG"
     gene            2053..3405
                     /locus_tag="ACZPCG_09605"
     CDS             2053..3405
                     /locus_tag="ACZPCG_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIR1285346.1"
                     /translation="MFNKILIANRGEIAVRIIRACREMGIQTVAVYSEADRDCLHTLL
                     ADEAICIGPAPSTQSYLNMERILSATVAMKADAIHPGFGFLSENARFAELCSKCNITF
                     IGPSAEIINRMGNKSEARKTMIEAGVPVVPGSKEAVYTAEDGLAMAKEIGFPVMIKAS
                     SGGGGKGMRISRSEEDFTEFFNAAQLESVKGFSDDTMYIEKYIEKPRHVEFQIMADKF
                     GNVIHLGERDCSIQRRHQKVLEEAPCSVISPELRRQMGETAVRAAKSVGYENAGTIEF
                     LLDKDKNFYFMEMNTRIQVEHPVTEMVSGLDLIKEQIRVAAGEPLSVTQDQVEIRGHA
                     IECRINAENPQKHFMPCPGRIQTVHLPGGNGVRVDTHIYSDYKVPANYDSMLMKLIVY
                     DKDRESAIAKMQSALGEVIIEGIETNINFQYEILENEAFRSGDTDTGFIEKYFPEYVK
                     "
     gene            3432..5192
                     /locus_tag="ACZPCG_09610"
     CDS             3432..5192
                     /locus_tag="ACZPCG_09610"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004607371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HIR1285347.1"
                     /translation="MLRNMFKKTYTKIDTIYRPKAGDSEEPSIPQGLWRKCNKCGQPI
                     YVEDVVNNYYVCPKCDGYFRIHAYRRIEMLADEGTFEEWNQEMPFSNPLDFPNYEKKV
                     AAAREKSHLNEAIVTGKAMIEGNPAVIGVCDARFIMSSMGHVVGEKITQAVEQATKEK
                     LPVILFACSGGARMQEGIVSLMQMAKTSAALKRHHEAGQLFISVLTDPTTGGVTASFA
                     MLGDIIIAEPHALIGFAGPRVIEQTIGQKLPEGFQRAEFLLEHGFVDMILERKDQKRV
                     IGQILYMHRKHGMTAVEPGMLRQESEPEAQKTHAAGSESFGHRSKAGEHHEKTAWDTV
                     LLSRKADRPTALDYINAVFDEFIEFHGDRYFKDDGAIVGGIAMFHGMPVTVIGQQKGK
                     NTKDNIKRNFGMPSPDGYRKALRLMKQAETFGRPIVCFVDTPGAFCGLEAEERGQGEA
                     IARNLFEMSDLKVPILSIVIGEGGSGGALAMAVADEVWMLENAIYSILSPEGFASILY
                     KDSKKAPEAAEVMKVTAADLKQLGLIEQIIPEKDPASTDTLYRIAGFMDQAMAGFFEA
                     YQAMTEEELAEHRYQRFRRM"
     gene            5192..6328
                     /locus_tag="ACZPCG_09615"
     CDS             5192..6328
                     /locus_tag="ACZPCG_09615"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003498417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
                     /protein_id="HIR1285348.1"
                     /translation="MSNFKPLVIGDLKIEKPVIQGGMGVGISLHRLAGAVAKAGGMGI
                     ISTAQIGFREPDFKTNFVEANLRAIRNEFKKAREIAPEGAIGFNIMVATKHYDMWVKE
                     AVKAGADAIISGAGLPVSLPEYAEAAYEEMKAVGKKLLRRTKLAPIVSTAKAAMVICK
                     MWDRKYHRVPDFVVVEGPLAGGHLGFTKEQLTQYGADSADVEKTYDQAAYDEEVKAIM
                     KVVKGYEEKYDTHIPVVTAGGIYTHEDVKHQFELGAEGVQVATRFVTTEECDAPDVYK
                     QAYIQASKEDIVITQSPVGMPGRAILNPFLKKIKEGERPAIRACFQCLEHCDIKTIPY
                     CITNALVNAAEGAEDTALLFCGSNAFRSERIETVEGVMNALLIG"
     gene            6454..7278
                     /locus_tag="ACZPCG_09620"
     CDS             6454..7278
                     /locus_tag="ACZPCG_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR1285349.1"
                     /translation="MADKKKEYLIGDVSRIVGLSRDALRFYEKKGVIRANKKENGYRY
                     FSDEDIYRLMCIVYRRKMNDSLEEIRESVKDNRSVAGMRRHLEQRIKEEEEEILRRRQ
                     SIARMSLSLKDMDCIEDCMGKYSLKKFPQAYILDTCEDIYDGLRQWFSMSSEVSGLDM
                     AYFYNMFSVEHESLENRGTQLLVYRGLEHFISKSFDLSRYPLTDTVPCIYTIESSKEL
                     LPDYAAAEKMTEWAEKRGITPSGMLYSNNMSSFLGEETDTYYLELYMPVIQSDQTP"
     gene            complement(7638..8300)
                     /locus_tag="ACZPCG_09625"
     CDS             complement(7638..8300)
                     /locus_tag="ACZPCG_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIR1285350.1"
                     /translation="MLQLNRLSHIVKRELKETFVPKTTVSIIIGTAITTFGLYNVHQQ
                     ADITEGGILGLILLLNFWLGMSSSLLSPVLDFLSYLMGFKYLGKEFLKTSIFATLCMA
                     GFFRLWELFPPLLPSLADIPFAASVLGGCFIGIGCGLVVRQGASCAGDDALALVISKI
                     TGWRISRAYLFTDLTVLILSLSYIPLKRIIYSLITVTISSYLIDFITRFGQTKENTAA
                     EV"
     gene            8443..9045
                     /locus_tag="ACZPCG_09630"
     CDS             8443..9045
                     /locus_tag="ACZPCG_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003498413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition protein"
                     /protein_id="HIR1285351.1"
                     /translation="MLNVLWSGMILSGILWAAVHGRLDQVTQGALASAKEAVSLCITM
                     LGVMSLWTGILEIGRGAGLIERLSRTMRPVLRFLFPHLSPDSEAARQISINFIANILG
                     LGWAATPAGLKAMEELKKEAEGSGHGGFAGDPVTATTDMCTFLILNISSLQLIPVNMI
                     AYRSQYGSPSPAAITGPCLAATAASTIAAVIFCKIMGRKK"
BASE COUNT         2556 a         1978 c         2530 g         2110 t
ORIGIN      
        1 cgccgtatgg agccattttc ccagtttgcg gtagctgcgg caaagcaggc cttagaccag
       61 tccggcattg acatggaaaa ggaggatccc ttccgtgtgg gcgtcagcgt aggttctggt
      121 atcggttccc tccaggccat ggagcgcagc gagaagaagc ttctggaaaa aggccccagc
      181 cgtgtagaac ctctgttagt tcctctgatg atctgcaata tggctgccgg aaatgtagcc
      241 atccagtttg ggcttaaggg caaatgcatc aatgtggtaa ctgcctgtgc aacgggtacc
      301 cactccatcg gagaagcctt ccgtgccatc cagtacggag aggcagacgt gatggtagca
      361 ggaggaaccg aggccagcat tactcccatc ggcattgcag gctttacctc cctgactgcg
      421 ttaaatacaa cagacgatcc aaaacgcgct tccattcctt ttgataagga cagaaacggc
      481 tttgtaatgg gcgaaggcgc cggcgttgtt gtattagagt ctttagagca tgcaaaagcc
      541 agaggcgcaa atatcctggc agaggtagtg ggctacggcg caacctgtga cgcataccat
      601 atcacttccc ctgctgagga cggcagcggc gctgcgaagg ccatggaatt tgccatgaag
      661 gacgcagaga tcatgccgga gcaggtagat tatatcaatg ctcacggcac cagcactcat
      721 cataatgacc tctttgagac aaaggccatc cgtctggcac tgggcgatca ggcagagaag
      781 gtgaagatta attctaccaa gtctatgatc ggtcatcttt tgggagcagc aggcggcgta
      841 gagttcatta cctgtgtaaa atccattcag gacggttatg ttcatgctac tcttggtctt
      901 gaggagccgg gcgagggctg cgatctggat tacactcagg gtgacggcgt agaaatgaat
      961 gtaaattatg ctatcagcaa ctcactgggc tttggcggac acaatgccag cctgatcgta
     1021 aagaaattta cagagtaagg aacaggagga catgaacatg aatatcgacg agatccttac
     1081 actggttcag gcggtgtctg attccagttt aaccagcttt aagctggaac agggagatac
     1141 ggttatttcc ctgagaaaag aaaaggaaga ggcaaagatt gtgaccgtag gcgctcccgc
     1201 agccccggct gtatttgcag cctccgcccc ggcccacgtt cttccggctc ccgagacagt
     1261 ggccgcttcc ggcgtacagg ttccggcagg tgtgcccgca ggacagacag cagctcctgc
     1321 agaggcgaag gatgcaggaa cggacattgg ttctgataaa gtaatcactt ctccgttagt
     1381 aggtactttc tatagctcct cttccccgga tgcagagcct tatgttaaag caggcgatac
     1441 cgtgaaaaag ggacagatcc tcggcatcat tgaggcaatg aagctgatga atgaaattga
     1501 gagcgaatat gacggcgtag tagaggcagt tcttgtaaac aacgaggatg tggtagagta
     1561 cggccagcct ctgttccgca ttaaataaag catacatcag gaggattacg atatgttaat
     1621 gggtattaaa gagattcagg aaatcattcc ccaccgccat ccattcctgc tggtggactg
     1681 cattgaggag ctggagcccg gcgtcagagc cgtaggctac aaggccatca cctatgatga
     1741 gtccttcttc agaggccatt tcccacagga gcccgttatg ccgggagttt tgatggtaga
     1801 ggcgctagct cagactggcg cagtggctat cctgtctcag gaaggcttta agggaaagac
     1861 cgcttatttc ggcggaattg acaagctgaa attcaagcac aaggtagttc cgggagaccg
     1921 tctgcgctta gagtgcgaga tcatccgcca gaagggcccg gtaggcatcg gaaaggccac
     1981 agctacggta gacggaaagg tagctgttac cggagagctg acctttatga tcggctgata
     2041 gggagaaata gaatgtttaa caagattttg attgcaaaca gaggtgagat cgcagtccgc
     2101 atcatccgcg cctgccgtga gatggggatt cagacggtag ctgtgtactc tgaggcagac
     2161 agagactgcc ttcacactct tttagcagac gaagccatct gtattggccc ggctccatct
     2221 actcaaagct atctgaacat ggagcggatc ctgtcggcaa ctgtagccat gaaggcagac
     2281 gccattcatc cgggctttgg cttcctgtct gaaaatgccc gttttgcaga gctgtgcagc
     2341 aagtgtaata ttacttttat cggcccctcc gcagagatca tcaaccggat gggaaataaa
     2401 tccgaggcaa gaaagaccat gatcgaggcc ggagttccgg tggttcccgg aagcaaggag
     2461 gctgtataca cagcagaaga cggacttgcc atggcaaagg aaataggttt tcctgtgatg
     2521 atcaaggcat cctccggcgg cggcggaaag ggaatgcgca tttccagaag cgaagaggat
     2581 tttacagagt tcttcaatgc tgcccagctg gagtctgtaa agggcttttc cgatgatact
     2641 atgtacattg aaaaatatat tgaaaagccc cgccatgtgg aattccagat tatggcagat
     2701 aagtttggaa atgtgattca tctgggagag agagactgct ccattcagag acgccaccag
     2761 aaggttttag aggaagcacc ctgcagcgtc atttctccgg agcttcgcag acagatgggt
     2821 gagacagctg tacgggctgc aaagtctgtt ggctatgaaa atgcgggaac catagaattc
     2881 cttttggata aggacaagaa tttctatttt atggaaatga acacccgtat ccaggtggag
     2941 catcctgtaa cagagatggt atccggcctt gatctgatca aggagcagat ccgcgttgca
     3001 gcgggagaac ccttaagcgt gactcaggat caggtagaga tccgcggcca tgccattgag
     3061 tgccgtatca atgcagagaa cccccagaag catttcatgc cctgtccggg acggatccag
     3121 accgtacatc ttccgggagg aaacggcgtc cgggtggata cccatattta cagcgactac
     3181 aaggttccgg caaactatga ttccatgctt atgaagctga tcgtatacga taaagaccgt
     3241 gaatctgcta ttgccaagat gcagagcgcc cttggcgagg tgatcatcga gggaattgag
     3301 acgaatatca acttccagta tgaaattctg gaaaatgaag cgttccgaag cggtgataca
     3361 gatacgggat ttatcgagaa atattttccg gagtatgtaa aataatcaga gattagaaag
     3421 gaagtagcct tatgctgcgt aatatgttca aaaaaacgta taccaagatc gatacgatct
     3481 acaggccgaa agccggagac agcgaagagc cctccattcc tcagggattg tggcgcaagt
     3541 gcaataagtg cgggcagccg atctatgtgg aggatgtagt gaacaattat tacgtctgtc
     3601 cgaaatgtga cggctatttc cgcattcatg cataccgccg gattgaaatg ctcgccgatg
     3661 aaggcacctt tgaggaatgg aaccaggaaa tgcccttctc caatcccctg gattttccaa
     3721 attatgagaa aaaggtggct gctgccagag aaaagagtca tttaaacgaa gcaatcgtca
     3781 caggtaaggc catgattgag ggaaatcctg ccgtcatcgg cgtctgtgac gcccggttta
     3841 ttatgagcag tatgggccat gtggtaggag aaaagattac ccaggcagta gaacaggcta
     3901 cgaaggaaaa gcttccggtg atcctctttg cctgttccgg aggagccaga atgcaggagg
     3961 ggatcgtttc tctgatgcag atggcaaaga cttccgctgc cttaaagcgc caccatgagg
     4021 ccggacagct ctttatcagc gtacttacag atccaaccac aggcggagtg actgcaagct
     4081 ttgccatgct gggcgatatt attatcgcag agcctcatgc cctgatcggc tttgcaggcc
     4141 ccagggtcat cgagcagacc atcggacaga agctgcccga gggctttcag agagcagagt
     4201 tccttttgga gcacggtttt gtagatatga tattggagag aaaagatcag aagcgtgtga
     4261 tcggccagat cctttatatg cacagaaagc acgggatgac cgctgtggag ccggggatgc
     4321 tgaggcagga aagtgagcct gaggcgcaga aaactcatgc agccggttct gaatcctttg
     4381 gtcatcgttc aaaagcagga gaacaccatg aaaaaacagc ctgggatacg gttcttctct
     4441 ccagaaaagc cgaccgtcct acggctcttg actatatcaa cgctgtattt gacgagttta
     4501 tcgaatttca cggagaccgt tatttcaagg atgacggggc gatcgtaggc ggtattgcca
     4561 tgttccacgg tatgccggtt acggtaatcg gtcagcagaa gggaaaaaat accaaagaca
     4621 atattaaacg caactttggt atgccttccc cggatggcta ccgcaaggcc ctgcgtctga
     4681 tgaagcaggc agaaaccttt ggacgtccca ttgtgtgctt tgtggataca cccggagcct
     4741 tctgtggcct ggaggcagag gagagaggac agggagaagc cattgcaagg aatctctttg
     4801 aaatgtcaga tctgaaggtt cccattcttt ccatcgtgat cggagaaggc ggaagcggcg
     4861 gcgctttggc tatggctgtg gcagatgagg tatggatgct ggaaaatgcg atctactcca
     4921 tcctgtctcc cgaaggcttt gcctccattc tctataagga cagcaaaaag gcccctgaag
     4981 cagcggaggt catgaaggta acagcggcag atttaaagca gctggggctg attgaacaga
     5041 ttattccgga aaaagatccc gcaagtacag ataccctgta tcgtattgcc ggatttatgg
     5101 atcaggccat ggcaggcttc tttgaagcct atcaggctat gacagaagag gaactggcag
     5161 aacacagata tcagagattt aggagaatgt gatgagtaat ttcaaaccat tggtaattgg
     5221 tgatcttaaa atagaaaaac cggtgatcca gggtggaatg ggggttggaa tcagcctcca
     5281 ccgtctggcc ggagccgtgg caaaggccgg cggaatgggg attatctcca ctgctcagat
     5341 cggttttaga gagccggatt ttaaaacgaa ctttgttgag gccaatctgc gtgcgatccg
     5401 taatgagttt aaaaaggcca gagaaattgc tcctgaggga gcaatcggct ttaacatcat
     5461 ggtagccaca aaacactacg atatgtgggt gaaggaggcc gtgaaggcag gagccgacgc
     5521 cattatatcg ggagcaggac ttcccgtatc tttgccggaa tatgcagaag cagcttatga
     5581 ggaaatgaag gcagtgggca aaaagctcct tcgccgtacc aagctggctc ccatcgtatc
     5641 gactgcaaag gctgctatgg tgatctgtaa gatgtgggac agaaagtatc acagggttcc
     5701 tgattttgta gtggtagaag gtcctctggc aggaggccat ctgggcttta caaaggagca
     5761 gctgacgcag tacggagcgg acagtgcaga tgtggagaag acctatgacc aggccgctta
     5821 tgatgaggaa gtaaaggcaa ttatgaaggt ggtaaaggga tatgaggaga aatacgatac
     5881 ccatatcccg gtggttactg ccggaggcat ttacacccat gaggatgtaa agcaccagtt
     5941 tgagctggga gcagagggag tccaggtcgc tacccgcttt gtgacaacgg aggaatgtga
     6001 tgcgccggat gtctataaac aggcgtatat tcaggcttca aaggaggata tcgtcatcac
     6061 tcagagcccg gtaggtatgc ccggaagagc gattttaaat cccttcttaa agaagatcaa
     6121 agaaggagaa cgtcctgcca tcagagcctg cttccagtgt ctggagcact gtgatattaa
     6181 gacgatccca tactgcatta ccaatgccct tgtgaatgcg gcagaaggag ctgaggatac
     6241 agcgcttctg ttctgcggaa gcaacgcctt ccgttccgag cggatcgaaa ccgtagaagg
     6301 cgttatgaat gcactgctga tcggatagaa tggtatgaaa aggagtcgga aaaggatccg
     6361 gctcttttta tttgcccccc tccgttgacc tgcttccgat ttgcaggtaa gatagaaacg
     6421 tcaaaaaatg ttaaaacagc aaggaaaaga aaaatggcag ataaaaagaa ggaatatctc
     6481 atcggagatg tgtcccgcat cgttggatta agccgggatg cgctgcgttt ttatgaaaaa
     6541 aagggcgtga tccgggcgaa caaaaaggaa aatggatatc ggtatttttc agatgaagat
     6601 atctatcggc tgatgtgcat tgtttaccgg agaaagatga atgacagtct ggaggaaatc
     6661 cgggaatcgg ttaaggacaa tagaagcgtt gccggtatga ggaggcattt ggagcagagg
     6721 atcaaggaag aagaggaaga aatcctcagg cgcaggcaga gtatcgccag gatgagcctg
     6781 tctttaaagg atatggactg tatcgaggac tgtatgggga aatacagcct gaagaagttt
     6841 ccgcaggcat atattctgga tacctgcgag gatatatatg atgggttgcg ccagtggttt
     6901 tccatgtcct ccgaggtcag cggcctggat atggcatatt tttacaatat gttctctgtg
     6961 gaacatgagt ctctggaaaa cagagggacg cagcttctgg tgtacagggg actggagcat
     7021 tttatcagta aaagctttga tctgtccaga tatccgctta cggatacggt accctgtatc
     7081 tatactatag agtcgtcaaa agagctgctt ccggattatg cggctgctga aaaaatgaca
     7141 gagtgggcag aaaaacgggg gattacgcct tccgggatgc tgtactccaa caacatgagt
     7201 tcttttttgg gagaagagac ggatacatat tatctggagc tttatatgcc cgtgatacag
     7261 tccgatcaga caccataaaa gggggaagac ccgccgggag aagcgtttct ccctggcggg
     7321 tctgcgtcat tactgacagt gtaaggataa aggatggagc tgctccaggg tcaatagggt
     7381 ttataaaaac gtaaagaaat gtaaatattc ttacagaaac agggaaaatt cgtcaaaaat
     7441 gtggataaat atttaacaat agaagctatt tccacataaa aatacatatt tccccatgtt
     7501 ttcccttgac tttccccaag gtataaacaa ggttatccac aaagttatcc acattatcca
     7561 ccgataaaca caacatatat ggtgtaaaat cgacagaaca caacatttag gttgtggata
     7621 acctgtaata cacaaaatta tacctcggca gccgtatttt ccttagtttg tccaaaacgg
     7681 gtgataaaat caatgagata cgatgaaatt gtaaccgtga tcaaagagta gatgatccgt
     7741 tttaagggga tataggacag ggacaaaata agaaccgtaa gatcggtaaa aagataggct
     7801 ctggaaatcc gccatccggt tatcttggaa attacaaggg ccagcgcgtc atctcccgca
     7861 caggaagcgc cctggcggac tacaaggccg cagccgattc cgataaaaca gcctcccagg
     7921 actgaagcgg caaaagggat atctgccagg ctggggagga gcggaggaaa aagctcccat
     7981 aaacggaaaa atccggccat acacaatgtg gcaaatatgg aagtctttaa aaattctttt
     8041 ccaagatact taaatcccat gagatagctg agaaaatcaa gtaccgggga cagcagagag
     8101 gaggacatgc caagccagaa attcagtaaa aggatcagtc ccaggatacc gccttcggta
     8161 atatcggcct gctggtggac attataaagt ccaaaggtgg taatggctgt gccaatgata
     8221 atagaaaccg tagttttggg aacgaaggtt tccttcagct cccgtttgac tatgtgtgac
     8281 agcctgttca gctgcaacat attaaaacat cctttcttga tatgatgctg ttattatagc
     8341 aggacagtgg ggtgaggtgt caaggggagg gggatcttaa aaggaatatt tatggatgtt
     8401 cgggcataga atatcaggca ggatagaaaa aggagaacgc gtatgctgaa tgtcctctgg
     8461 tcaggaatga tcctgtcggg aattttgtgg gcggcggtcc atggaaggct ggatcaggtt
     8521 actcagggag cgctggcctc ggcaaaagag gcggtatcct tatgcattac aatgctggga
     8581 gttatgtcgc tgtggacggg aatactggaa atcggccgcg gagcgggact gatcgaacgt
     8641 ctttccagga cgatgaggcc ggtgctgcgt ttcctttttc cgcatctttc tccggacagt
     8701 gaggcggcca gacagatttc cattaatttt attgccaata tattagggct tggctgggca
     8761 gctacccctg cgggattaaa agctatggaa gaattaaaaa aagaagcgga gggatccggt
     8821 catggcggct ttgcaggtga tccggttaca gcaaccacgg atatgtgcac ctttctgata
     8881 ctgaatatct cctccctgca gctgatccct gtaaatatga tcgcctatcg gagccagtac
     8941 ggaagcccgt ctcctgcggc gataaccggg ccgtgtctgg cggctacagc cgccagtaca
     9001 attgcagctg tcatattttg taagataatg ggaagaaaaa aatgacgccg gtgatgacac
     9061 gcggcgtcat ttgaatatgg tttatttttc gtaagtgtaa ttttctacct ccaccttatc
     9121 gacttcctga taaggagcca gagtggagat cattttaacg ggaacgtcat aatt
//
LOCUS       DBMDUH010000112         9025 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_3573_length_9025_cov_4.732519, whole genome shotgun sequence.
ACCESSION   DBMDUH010000112 DBMDUH010000000
VERSION     DBMDUH010000112.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 9025)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9025)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9025
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3573_length_9025_cov_4.732519"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            271..2577
                     /locus_tag="ACZPCG_09635"
     CDS             271..2577
                     /locus_tag="ACZPCG_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HIR1285352.1"
                     /translation="MVSLYEGGVYLVNGTELVPEKEAGKVEALTGKAADRETAKKGTI
                     AYSILKAHNTTEDMEHLKLRFDSMASHDITFVGIVQTAKASGMEQFPIPYVLTNCHNT
                     LCAVGGTINEDDHMFGLSAAKKYGGIYVPPHIAVIHQYMRERMAGCGKMILGSDSHTR
                     YGALGTMAVGEGGGELVKQLLKDTYDVSYPGVVAVYLDGAPRPGTGPQDIALAIIGAV
                     FKNGYVKNKVMEFVGPGVSSMTTDFRNGVDVMTTETTCLSSIWRTDEDTRDYLAKHHR
                     EEDYRQLDPADVAYYDGCVYVDLSTVKPMIALPFHPNNTYEIDELKENLTDILALVEK
                     EAARISGGRASFTLLDKVQGKDLMVQQAVIAGCAGGNYTNVMEAAQALRGRNCGCGEF
                     SLAVYPSSQPVFADLVKKGAVSDLMNAGAIVRTAFCGPCFGAGDTPCNNGLSIRHTTR
                     NFPNREGSKPGNGQMAAVALMDARSIAATAANGGRLTSAWEMDGWDQVPEYEYDDTSY
                     RNRVYTGFGAGDASADLVYGPNIKDWPEMSPLTDNILLKVCSKILDPVTTTDELIPSG
                     ETSSYRSNPLGLAEFTLSRRDPEYVGRAKEAGALETARRTRGTGAQLEAEPALEGIFA
                     AIRTISGNEMVKMDDTELGSMVYAVKPGDGSAREQAASCQRVLGGLANICKEYATKRY
                     RSNVINWGMLPFQMEEEPDFEVGDYIYVPGIKAALEGDLKEIPAYVLGKDGTAPIKEI
                     RLYMADMTAEERQIVKAGCLINYNRNRH"
     gene            2598..3941
                     /locus_tag="ACZPCG_09640"
     CDS             2598..3941
                     /locus_tag="ACZPCG_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HIR1285353.1"
                     /translation="MTRESYLAQFYGKSQNYTDIPNKWYKEYNVKKGLRNEDGTGVRV
                     GLTRVADVVGYSEGADGQIVAIPGELYYRGYNVVDLVKGKLNSHFGYEEVCFLLLFGY
                     LPKRKELDQFCAILRDCYEIPDEFVEMNLLRMPGKNLMNKLQDAVLTLYNYDDDPDNT
                     DVYETLVKGINLVAKLPSIACYAYQSKVHYYNRDSLFIHYANPEYSIAENFLSVLRKD
                     QKFTDKEASLLDTILMLHADHGGGNNSTFTNVVISSTGTDIYSTVSASIGSLKGPRHG
                     GANIKVAEMMDAVLDEVGGYTADDKKLRAAVHKLMDGKLYDGSGLVYGFGHAVYTVSD
                     PRADMLRACCAEVAKEKKQMTEFDFRQRFENAALEVMKERKGVALPTNVDYYSGFAYG
                     MLNIPRDLYTPLFMCSRMVGWVAHNIENKLYDGRIMRPATKYVGDNKDYMPMEER"
     gene            3945..4946
                     /locus_tag="ACZPCG_09645"
     CDS             3945..4946
                     /locus_tag="ACZPCG_09645"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012407.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HIR1285354.1"
                     /translation="METNTITVFKGDGIGPEITDAVLAILDAAGADLDYEIFRVGAAE
                     YEENGALIPEEGFASFEKTHVLLKSPITTPLGTGFRSLNVTLRKKYDLYANMRPAKSN
                     AAVKTPFEHVDIVMFRENTEDLYAGVEEKIDENTAHSIKIITRKCSERIVRSAFDYAV
                     KNKRKKVTCVHKANIMKLSDGLFRDVFYEISREYPGIEAEDKIVDNVAMQLVMHPETF
                     DVIVTENLYGDILSDLTSGLIGGLGLLPSSNMGTDFAMFEAVHGSAPDIAGKGIANPT
                     AFLWSACMMLEHLGKQEISAKIRRAVDEVLKDGTILTPDLGGCAGTEAYTKAIIERL"
     misc_binding    5029..5265
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5347..7761
                     /gene="leuS"
                     /locus_tag="ACZPCG_09650"
     CDS             5347..7761
                     /gene="leuS"
                     /locus_tag="ACZPCG_09650"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003525299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HIR1285355.1"
                     /translation="MASQYNHSAIEKKWRENWEKNPINVNDGTKEKYYCLDMFPYPSG
                     SGLHVGHWRGYVISDVWSRYQLLRGKYVIHPMGWDAFGLPAENYAIKMGVHPAKSTEA
                     NITNIKRQIKQIAAIYDWDMEVNTTDPEFYKWTQWIFVQMFKKGLAYEKEFPINWCPS
                     CKTGLANEEVVNGCCERCGTPVTKKNLRQWMLKITAYAERLLNDLDKLDWPEKVKKMQ
                     ADWIGKSYGAEVEFPIDGREEKITVYTTRPDTLHGATFMVLAPEHKLAKSLATDETRE
                     AVEKYIFDSSMRSNVDRMQAKEKTGVFTGSYAINPLNGAKTPIWLSDYVLADYGTGAI
                     MCVPAHDDRDFEFAKKFGLPIVQVIAKDGKEIENMTEAYTEASGTMINSGEWNGMESS
                     VLKKEAPQMIEEKGFGRKTVNYKLRDWVFSRQRYWGEPIPIIHCPKCGCVPVPEDQLP
                     LRLPEVESYQPTGTGESPLAAIDEWVNTTCPVCGAAAKRETNTMPQWAGSSWYFLRYV
                     DSHNSEALVSREKADKYLPVDMYIGGVEHAVLHLLYSRFYTKFLCDIGVVDFDEPFKK
                     LFNQGMITGKNGIKMSKSKGNVVSPDDLVRDYGCDSLRLYELFVGPPELDAEWDDRGI
                     EGVSRFLNRFWNLVMDSKDKDIQPTKEMIKLRHKLVYDIEQRFNQFSLNTVISGFMEY
                     NNKFIELAKKEGGIDKETLKTFVTLLAPFAPHIGEELWSQLGGTDSVFHSQWPECDEE
                     AMKDDEIEIAVQVNGKTRAVINVPADIAKDDVIAQGREAVKDKLAGNIVKEIYVPGRI
                     VNIVCK"
     gene            7865..8446
                     /locus_tag="ACZPCG_09655"
     CDS             7865..8446
                     /locus_tag="ACZPCG_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HIR1285356.1"
                     /translation="MDQIHVKKRDTGIKAKRLRQLGMVPGNIIGKSLPESISIQMEEA
                     EARRLVRQLREGSKIAIDLEGQAIPAQIKEKSLNTLNNEILHLSFQALVADEKVNSVI
                     HILLENEEKAGNQLEKMMMEIPYASLPADMIDTITIDLDGMAVGTVVMVKDIPELMSD
                     TIELRVDPEEIVLRISDKTYQDSQADTDGSEET"
     gene            8555..8812
                     /locus_tag="ACZPCG_09660"
     CDS             8555..8812
                     /locus_tag="ACZPCG_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285357.1"
                     /translation="MFGFSKKEIFMNMDPELFFRITAALKEHGIRYETKHVNGTTVNW
                     MTGTAASVGETLRRQTSYYAYVDKKDAEEAAYIVKKALERR"
     gene            8818..>9025
                     /locus_tag="ACZPCG_09665"
     CDS             8818..>9025
                     /locus_tag="ACZPCG_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285358.1"
                     /translation="MYKRKRMGTAVGVCICAALMFGTATAFAAGQAETGAMSGGPGEI
                     KTHDEKKTQDQFQNTDQLILVKGTV"
BASE COUNT         2601 a         1847 c         2447 g         2130 t
ORIGIN      
        1 aaatttacag atggattcac ctttttcata ttccggcatg aggataacag ccgccgcata
       61 cactgtcaaa aagccaaaac agatatttta acggtgtgaa gtgatagtaa ataacagtgc
      121 tgagtacagg agattcggtt ttgccacaac taaaaagctg tatcaattac aacttttcaa
      181 ctggacaaat aagcatctgt gttttatagt ataaaaaaca acgaattcac agatttggaa
      241 acagacagga gaatccggga ggtagagagc atggtatcat tatatgaagg cggcgtttat
      301 ctggtaaatg gaacagagct ggttccggaa aaggaagcag gaaaggtaga ggcactaact
      361 ggaaaagcgg cagaccgtga gactgccaaa aagggtacga ttgcctattc tattttaaag
      421 gcgcacaaca caacggagga catggagcac ttaaaacttc gctttgacag tatggcttcc
      481 catgatatta cctttgtggg gatcgtccag acagccaaag cctccgggat ggagcagttt
      541 cctattccct atgtattgac gaactgtcac aacacgctct gcgcagtagg cggcaccatc
      601 aatgaggatg atcatatgtt tgggctttct gcggcaaaga agtacggcgg tatctatgta
      661 cctccgcaca ttgcggtgat ccatcagtac atgagagagc ggatggcagg ctgcggaaag
      721 atgattttag gctccgacag ccatacccgc tatggcgctc tcggcaccat ggcagtagga
      781 gagggcggcg gagagctggt aaagcagctt cttaaggata cctatgatgt gtcttatccc
      841 ggcgtagttg ccgtatatct ggacggagcg ccaagaccgg gaaccgggcc tcaggatatt
      901 gcccttgcga ttatcggagc tgtatttaaa aatggatacg tgaaaaataa ggtgatggaa
      961 tttgtaggcc ccggcgtttc ttccatgaca acggatttca gaaacggcgt agatgtgatg
     1021 accacagaaa ctacctgttt aagctccatc tggcgcactg atgaggacac cagggactat
     1081 ctggcaaagc atcacagaga agaggattac agacagctgg atcccgctga tgtggcttac
     1141 tatgacggct gtgtgtatgt ggatctctct acggtaaagc ccatgatcgc tctgccgttc
     1201 catcccaaca atacatatga gattgacgag ctgaaggaaa atctgacgga tattttggct
     1261 ctggtggaaa aggaagctgc aaggatttca ggcggccgtg catcctttac cctgcttgat
     1321 aaggtacagg gaaaagattt gatggtacag caggcagtga ttgcaggctg cgccggcgga
     1381 aattatacaa atgtaatgga agcggctcag gcccttcggg gaagaaactg cggctgcggc
     1441 gagttctccc tggcagtcta tccttcttct cagcctgtat ttgcagatct ggtaaagaag
     1501 ggagctgttt cggatctgat gaacgcggga gccattgtcc gcaccgcctt ctgcggcccc
     1561 tgctttggcg caggtgatac gccctgcaat aatggtttaa gtatccgcca cactaccagg
     1621 aatttcccta accgggaagg ctccaagcct ggaaacggac agatggcagc agtagcgctg
     1681 atggatgccc gttccattgc ggcaacagcg gcaaacggag gcagactgac ttctgcctgg
     1741 gagatggacg gctgggatca ggtgcctgag tatgaatatg acgatacttc ttatcgaaac
     1801 cgggtataca caggctttgg agcgggtgat gcgtctgcag atctggtata cgggccgaat
     1861 atcaaagact ggccggagat gagcccgctg acagacaata ttttattaaa ggtatgttca
     1921 aagattctgg atcctgtgac gactacggat gagctgattc cctccggcga gacctcatcc
     1981 taccgctcca atcctctggg gctggcagag tttactttat ccagaaggga tccggagtat
     2041 gtgggccgcg ccaaggaggc gggagctctg gaaacagcca gaagaaccag gggcaccgga
     2101 gcgcagctgg aagcagaacc ggctcttgaa ggtatctttg cagccatccg taccatttcc
     2161 ggaaatgaaa tggtaaaaat ggatgacaca gagcttggaa gcatggttta tgctgtaaaa
     2221 ccaggcgacg gatctgccag agagcaggcg gccagctgcc agagagtgct gggcggtctt
     2281 gcaaatatct gtaaggagta tgcgaccaag cgctaccgct ccaatgtaat taactggggt
     2341 atgcttccct tccagatgga ggaggagccg gattttgaag tgggtgatta catttatgta
     2401 cccggaataa aagctgccct ggaaggagat ttaaaggaaa ttccggcgta tgttctggga
     2461 aaagacggaa ctgcaccgat aaaagaaatc aggctttata tggcagacat gacggctgaa
     2521 gaacggcaga tcgtaaaggc cggatgtctg atcaactata acagaaaccg tcattaaaat
     2581 cagaaaggag aaccgccatg acaagagaaa gctatcttgc acagttttat ggaaagtctc
     2641 agaattatac agatattcct aataaatggt ataaggaata caatgtaaag aaaggtctga
     2701 gaaatgagga tggcacaggt gtccgtgttg ggcttacacg ggttgcagat gttgtaggat
     2761 acagtgaggg agcggacgga cagatcgtgg cgattcccgg agagctttat tacagaggct
     2821 acaatgtggt cgatctggta aagggaaagc tgaacagcca tttcggctat gaggaggtat
     2881 gcttccttct gctttttggg tatcttccaa aaagaaagga gctggatcag ttctgtgcaa
     2941 tcttaagaga ttgttatgag ataccagatg aatttgtgga aatgaacctg ctgcggatgc
     3001 cgggaaagaa cctgatgaac aagctgcagg atgcggttct taccctgtat aattatgatg
     3061 acgatccaga caatacagat gtatatgaaa cgctggtaaa aggaatcaac ctggtggcaa
     3121 agctgccgtc cattgcctgc tatgcctatc agagtaaggt tcattattat aacagagaca
     3181 gcctctttat ccattatgcc aatccggaat attccattgc agaaaatttc ctgagtgttc
     3241 tgagaaaaga tcagaagttt acggataagg aagccagcct cttagatacg atcctgatgc
     3301 ttcatgcgga tcatggcggc ggaaataact ctacttttac caacgtggta atttcctcta
     3361 cagggacaga tatttattcg accgtatctg ccagcatcgg ttctttaaaa ggccccagac
     3421 acggaggcgc caatatcaag gtggcagaaa tgatggatgc agttttggat gaagtcggag
     3481 gctatacggc agatgataaa aagctgcggg ctgcagtcca taagctgatg gatggaaagc
     3541 tgtatgacgg ttctggtctg gtatacggtt tcggccatgc ggtttatacg gtttccgatc
     3601 cccgtgcgga tatgctcaga gcctgctgtg cagaggtggc taaggagaaa aagcagatga
     3661 cggaatttga cttccgccag aggtttgaaa atgctgccct ggaggttatg aaggagagaa
     3721 agggtgtggc tctgcctacc aatgtagact attacagtgg ttttgcttat ggaatgttaa
     3781 atattcccag agacctgtat acaccattat ttatgtgcag ccgtatggta ggctgggtgg
     3841 cccacaatat cgaaaacaag ctgtatgacg gacggatcat gcgtcctgct accaagtatg
     3901 taggggacaa taaggactat atgccaatgg aggagagata agtcatggaa acgaatacaa
     3961 tcacagtatt taagggagac ggcattggac cggagattac agatgcagtg cttgcaatcc
     4021 tggatgcggc aggtgcagat ctggattatg agattttccg tgtaggagcg gcagagtatg
     4081 aggaaaatgg tgctctgata ccggaagaag gctttgcttc ctttgaaaag acccatgtgc
     4141 ttttaaaatc tccgatcacg actccattag gaaccggctt ccgttcgctg aatgtaacgc
     4201 tgcgcaaaaa atacgatctt tacgccaata tgcgccctgc aaagtctaac gctgcagtta
     4261 agacgccctt tgaacatgtg gatattgtaa tgttcagaga aaacacagag gatctctatg
     4321 caggcgtgga agaaaagatt gatgagaata cggcccattc cattaaaatc attaccagaa
     4381 aatgcagtga gcgcattgtc cgatccgcct ttgactatgc ggtaaagaac aaaagaaaaa
     4441 aagtaacctg cgtccataag gctaatatca tgaagctttc cgacggcctg ttccgtgatg
     4501 ttttttacga gatcagccgg gaatatcctg gaattgaagc agaggataag atcgttgata
     4561 atgtagccat gcagctggta atgcatccgg aaacctttga cgtaatagtg acagaaaatc
     4621 tgtatggaga tattctgtcg gatcttacca gcgggctcat cggcggactg ggtcttctgc
     4681 cctccagcaa tatgggaacg gactttgcca tgtttgaggc agttcacggc agcgcccccg
     4741 atatcgcagg aaagggaatt gccaatccca ctgcattttt gtggtccgcc tgcatgatgt
     4801 tagagcatct gggaaagcag gagatttctg caaaaatccg aagagcagtg gatgaggtat
     4861 taaaggatgg aacgattcta acgccggatc tgggcggatg cgctggaacg gaagcatata
     4921 caaaggcaat tatcgaacgc ctgtaagttt ttggcggtat aaacagccac ggcttttttt
     4981 gacagtatat tgacagccat tgtcaaatgt gttaaattaa gattcagtat aaatgcattg
     5041 acaggaagta gtacgtattt attttgtctc acagagagcc ggtagaggct gagagccggc
     5101 ggcaggagga tactgaactg gtcctggagc agcttttctg aagggcattt gccagtctga
     5161 gtaggaaaag ccggagtgcc caccgttaca ggggcagatg agtgcgcatg ggcttaccat
     5221 gcgaatcaga gtggtaccgc gtggaactat gaccgcgtct cttgaagcaa tttaagagat
     5281 gcggtttttt gttttacagg aaagtgtgtc cagctctgag cactaatatt aacggaggaa
     5341 tgaattatgg catcacagta caatcacagc gccattgaaa agaagtggcg tgagaactgg
     5401 gaaaaaaatc cgatcaatgt aaatgacgga acgaaagaaa aatactactg cctggatatg
     5461 ttcccgtatc catctggaag cggccttcat gtaggtcact ggagaggcta tgtaatctct
     5521 gatgtatgga gccggtatca gctgttaaga ggcaaatatg tgatccatcc tatgggatgg
     5581 gatgcatttg gacttccggc agaaaattat gccatcaaga tgggcgttca tcctgcaaag
     5641 tccacagagg ccaacattac caatatcaag cgccagatca agcagattgc tgcaatctat
     5701 gactgggata tggaagtaaa taccacagat cctgagtttt acaagtggac gcagtggatc
     5761 tttgtgcaga tgtttaaaaa gggacttgcc tatgagaagg aattccctat caactggtgc
     5821 ccttcctgta agaccggcct tgcaaacgaa gaggtggtaa acggctgctg cgagcgatgc
     5881 ggaacccctg ttacaaagaa gaacctccgt cagtggatgt taaagatcac ggcctacgca
     5941 gagcgcctgt taaatgatct tgacaaatta gactggccgg aaaaggttaa gaagatgcag
     6001 gcggactgga tcggcaaatc ctacggcgcc gaggtggaat tccccattga cggcagagag
     6061 gaaaagatca ccgtttatac tacaagacct gatacccttc acggcgcaac ctttatggtt
     6121 ctggctccgg agcacaagct ggcaaagagc ctggctaccg atgagacaag agaagccgtt
     6181 gaaaaatata ttttcgattc ctccatgcgt tccaacgtag accgtatgca ggcaaaggaa
     6241 aagaccggcg tttttacagg cagctatgcc ataaacccct taaatggagc aaagacgcca
     6301 atctggctgt ccgattatgt actggctgat tacggcacag gtgctatcat gtgtgtacct
     6361 gcccatgatg acagagactt tgaatttgct aagaaattcg gccttcctat cgttcaggta
     6421 atcgcaaagg acggcaagga aattgaaaat atgactgaag cctatacaga ggcaagtgga
     6481 accatgatca attccggcga atggaacggc atggagtcct ctgttctgaa aaaagaagct
     6541 cctcagatga tcgaggaaaa gggctttgga cgcaagacag tcaactacaa gctgcgcgac
     6601 tgggtattct cccgtcagag atactggggc gagcccattc cgatcatcca ctgtccaaag
     6661 tgcggctgcg tgcctgttcc ggaggatcag cttccgctgc gtctgcctga ggtagagagc
     6721 tatcagccta ccggtaccgg tgaatctcct ctggctgcca ttgacgagtg ggtcaatacc
     6781 acctgtccgg tctgcggagc tgctgcaaag agagagacca ataccatgcc tcagtgggcc
     6841 ggttcctcct ggtacttcct gcgctatgtg gacagccaca acagtgaggc ccttgtttcc
     6901 agagaaaagg cagataaata ccttcctgta gatatgtata tcggtggcgt agagcatgcg
     6961 gtgcttcacc ttctgtattc ccgtttctac accaaattcc tttgcgatat cggtgttgtc
     7021 gattttgatg agccctttaa gaagctcttt aaccagggta tgatcaccgg aaagaacggc
     7081 atcaagatga gcaagtccaa gggaaatgtc gtttccccgg atgacctggt aagagattac
     7141 ggctgtgatt ccctgcgtct gtacgagctg ttcgtaggac cgccggagct ggatgcagag
     7201 tgggatgacc gagggatcga gggcgtttcc cgtttcttaa atcgcttctg gaacctggtc
     7261 atggacagca aggataagga tatccagccg acaaaggaaa tgatcaagct ccgccataag
     7321 ctggtatatg atattgagca gagattcaac cagttcagct taaataccgt tatttctggc
     7381 tttatggagt acaacaacaa gtttatcgag ctggcaaaga aggaaggagg aatcgataaa
     7441 gaaaccttaa agaccttcgt tactctcctt gctccatttg cacctcatat cggcgaagag
     7501 ctgtggagcc agctgggcgg cacagacagc gtattccatt cccagtggcc ggagtgcgat
     7561 gaggaagcca tgaaggacga cgagattgag atcgctgttc aggtaaacgg caagacccgc
     7621 gcagtcatca acgtacctgc tgatattgca aaggatgatg tgatcgctca gggaagagaa
     7681 gcggtaaagg acaagctggc aggaaatatc gtaaaagaga tttacgttcc tggaagaatt
     7741 gtaaatattg tctgcaaata atttgagaaa aagagaggag attttcgtat gttgcgaaag
     7801 gtctcctctt ttttaggcat atttcgccgc acacggcata tatagtctat aaggaggcga
     7861 aattatggat cagattcatg taaaaaagag agatactgga ataaaggcaa agcggttgag
     7921 acagttgggg atggttccgg gtaatattat tggaaagtcc cttcctgagt ccatttctat
     7981 tcagatggag gaggcagagg cccgcaggct ggtgcggcag ttaagagagg gaagtaagat
     8041 tgccattgac ttggagggac aggctattcc ggcgcagatt aaggaaaaaa gcctgaacac
     8101 gctgaataac gaaatccttc acctcagctt tcaggcgctg gttgcagatg agaaggtaaa
     8161 cagtgtaatc cacattctgc ttgagaatga agaaaaggct gggaaccagc tggagaagat
     8221 gatgatggag atcccatatg catctctccc ggctgacatg atcgatacga ttaccattga
     8281 cctggatgga atggcggtag gcacagtggt aatggtgaag gatattcctg agcttatgag
     8341 tgatacgatt gaactgcggg tggatccgga ggaaattgtt ctgcgtatca gtgataaaac
     8401 atatcaggat tctcaggcag atacagatgg atcagaagaa acatagaaaa cagaaacaga
     8461 tcgtcaaaac agaggcatac attcaggaaa ctgtaagaga ttttcggatc aatctatggt
     8521 agaatgaaat catcagagag gagtgtgtgc ttctatgttt ggtttttcaa agaaagaaat
     8581 atttatgaat atggatccgg agcttttctt tcggataaca gcagcgctta aggaacatgg
     8641 aataagatat gaaacaaaac atgtaaacgg tactactgta aattggatga ctggtactgc
     8701 ggcttctgtg ggagaaactc tcagacgcca gacctcgtac tatgcctatg tagataaaaa
     8761 ggatgcggaa gaagctgcct atattgtgaa aaaggcgctg gagcggaggt aggagatatg
     8821 tacaaaagaa aaaggatggg cactgctgtt ggagtatgca tctgtgcagc attgatgttt
     8881 gggacggcaa cggcttttgc agcaggacag gctgagacag gggcaatgag cggcggaccc
     8941 ggtgagataa aaactcatga cgagaaaaaa acgcaggatc agtttcagaa tacagatcag
     9001 ttgatccttg ttaaaggaac agtcg
//
LOCUS       DBMDUH010000113         8853 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_3635_length_8853_cov_6.321984, whole genome shotgun sequence.
ACCESSION   DBMDUH010000113 DBMDUH010000000
VERSION     DBMDUH010000113.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 8853)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8853)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8853
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3635_length_8853_cov_6.321984"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /gene="yqeC"
                     /locus_tag="ACZPCG_09670"
     CDS             complement(<1..506)
                     /gene="yqeC"
                     /locus_tag="ACZPCG_09670"
                     /inference="COORDINATES: protein motif:HMM:TIGR03172.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HIR1285359.1"
                     /translation="MGEDGGQNVEAYYYGACLTQAVHSIFKALEIEKYRLSGCPFVIS
                     FTGGGGKTSLIRRMAWEGRELGLKVLVIPTTHMALPEHFFVPEPSVGALEKTLKDSGI
                     AVAGNPEERKPGEKKISFWGWEFYQQAREKADLVLVEADGAKRLPVKVPGPAEPVIPG
                     DTDLILCV"
     gene            complement(696..860)
                     /locus_tag="ACZPCG_09675"
     CDS             complement(696..860)
                     /locus_tag="ACZPCG_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285360.1"
                     /translation="MSTMQIAVISFLGYNNSIIRNRRGRKKMGFIAVVIVFASLGYDM
                     YRVIRSHGNN"
     gene            1032..1328
                     /locus_tag="ACZPCG_09680"
     CDS             1032..1328
                     /locus_tag="ACZPCG_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HIR1285361.1"
                     /translation="MSVVIIGGHDRMVTQYKKICKTYNCKAKVFTQMSASLDKQIGTP
                     DLLVLFTNTVSHKMIRCALDEVRPDTTQIVRSHTSSASALNEILSQRTAACSSI"
     gene            complement(1325..2476)
                     /locus_tag="ACZPCG_09685"
     CDS             complement(1325..2476)
                     /locus_tag="ACZPCG_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR1285362.1"
                     /translation="MIYLDSAATSFYRPPVVARAVAEAMNSMGNCSRGTHREALTGAR
                     IIFETRQLLAELFHAPGPQQVAFTANSTESLNMAVKGLIDPGDRVVTTVLEHNSVLRP
                     LYEMEKRGAVLTIVGCEKDPSNGISRKGRLDMIAMEKAITPGTKAVVMTHASNLTGNC
                     QDLAQVGEWCRRAGALFIVDASQTAGLFPIDMERDQIDVLCFTGHKGLMGPQGTGGIC
                     LGTGVNIRPLLSGGSGIMTFEKQHPSQMPTALEAGTLNGHGIAGLRAALLYIKEQGME
                     HLRKREQQLMRRFYEQALEIPDIRIYGDFSQKDRAAIVSLNLGEEDSAQVSDYLSEEY
                     DIATRSGGHCAPLMHEALKTVEQGAVRFSFSHFNTEEQIDEAIDALKHF"
     gene            complement(2489..3313)
                     /gene="yqeB"
                     /locus_tag="ACZPCG_09690"
     CDS             complement(2489..3313)
                     /gene="yqeB"
                     /locus_tag="ACZPCG_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007712791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HIR1285363.1"
                     /translation="MNRQKQLIIVRGGGDIATGTIHRLHRCGYPVLVLETGSPSAIRR
                     QVAFSEAIYDGQSEIEGVTCLKVSDFAEAKAVLEAGAIPMMMDETCEILKEVRPWALV
                     DAILAKKNLGTNRNMADKTIGLGPGFAAGEDVDLVIETMRGHNLGRIISRGTALPNTG
                     TPGLIGGAGKERVIHSPAMGILFGKVRIGDRVEKGQPVAVIVTGQGEVVVEASLTGLV
                     RGLIRDGYPVAKGFKIADIDPRESEYENCFTISDKARAIGGSVLEALLYLEQKQGF"
     gene            complement(3324..3959)
                     /gene="yedF"
                     /locus_tag="ACZPCG_09695"
     CDS             complement(3324..3959)
                     /gene="yedF"
                     /locus_tag="ACZPCG_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HIR1285364.1"
                     /translation="MRLDERGKQCPLPVIEAKRALEAAAPGEAVEVVVDNEIAVQNLK
                     KLADHKGLSFASEKKSSREYVAVITAGEGVKEGESAPQTDALPEKEPEVCHPDGRRKG
                     MVVVISSREMGQGDPALGTLLMKGFIYALTQQDVLPETILLYNGGAFLSCEGSDNLED
                     LKTLEAQGVEILTCGTCLNHYGIGDRLRVGSVTNMYEIAERMTGAAVLVRP"
     gene            complement(3988..5010)
                     /gene="selD"
                     /locus_tag="ACZPCG_09700"
     CDS             complement(3988..5010)
                     /gene="selD"
                     /locus_tag="ACZPCG_09700"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HIR1285365.1"
                     /translation="MSEEIVFCRGGGCTAKLGPGVLARVLNRLPKTKDPNLMIGYDGS
                     DDAAVYRLTDDLAMVQTLDFFPPMVEDPYIFGQIAAANALSDIYAMGGEVKTALNIVC
                     FPEEMDLNILGKIMQGGSEKVREAGGVLAGGHSIADSDVKYGLSVTGIIHPDRVFANS
                     DCKEGDALILTKPLGVGIVCTAARMKAAPQAAYDLAVQSMTTLNKYASEILKKYRLHG
                     CTDVTGFSFLGHLCEMVTDQATARIYKDRIPVIPEAPALAEEFYITAAAQRNRNHVQD
                     KIDFGSCGFSMQEILFDPQTSGGLLASIDAADAEAALKELEELGLPCGIVGRIEARQE
                     KAVIVE"
     gene            complement(5003..5398)
                     /locus_tag="ACZPCG_09705"
     CDS             complement(5003..5398)
                     /locus_tag="ACZPCG_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007712583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR1285366.1"
                     /translation="MEDNNKLHYGLSLRLYFEEKAFGPGMVALLRAVEKTGSLQRAAR
                     SMNMAYSKAWKMLRVAEKEWGFLLTDRETGGRDGGGSTLTPQAKELMEAYLSFRRDAE
                     AELDRLFETYFSDEWLNHMREMGGNEKNE"
     gene            5593..6429
                     /locus_tag="ACZPCG_09710"
     CDS             5593..6429
                     /locus_tag="ACZPCG_09710"
                     /inference="COORDINATES: protein motif:HMM:NF028234.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HIR1285367.1"
                     /translation="MRTFFAVGGDMRLRWMVQILSKEGYPVSFFTGTDSPLMKSAVKE
                     ASVILGPVPFTRDGVHLFQPPSHGMELSALLSCLMPGQYLFGGNLPDPVKEFCDNNDI
                     LWKDFMDMEEISLENAIATAEGAISLAVSQCPINLHRCRCLILGYGRCGQALADRLRG
                     MFADASVCEINPIRQTLAKTRGFDTFDPQKLEKYLPQAELIFNTVPAPILTDPLLIKQ
                     IRKDAVLFELASGSGCISEEACRQAGLCRIACPGLPGRFSPKASARILCRAVLQVLGE
                     GR"
     gene            6463..7050
                     /locus_tag="ACZPCG_09715"
     CDS             6463..7050
                     /locus_tag="ACZPCG_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HIR1285368.1"
                     /translation="MSLKGKTIGLAVTGSFCTFDKIKQTVQTLIQEEAELYPIFSSHV
                     QTTRSRFGSTDEYIRTISGLAGREPILTIEDAEPIGPKGYLDLLLIAPCTGNTLAKLA
                     NGITDTPVLMAAKAHLRNEKPLVIFLSTNDAMGMNFKNIGLLFPCKHVYFVPFSQDSP
                     QKKPCSLVARTELVIPTIKAALKGEQLQPVLYGPD"
     gene            complement(7063..7824)
                     /locus_tag="ACZPCG_09720"
     CDS             complement(7063..7824)
                     /locus_tag="ACZPCG_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009255252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR1285369.1"
                     /translation="MKECVWAIYFSPTGGTKKAAFYLAESLGKEVKEVDLSAPDFNGA
                     EFKKDDLVIVAAPAFGGRIPALMAERLKCTAGKGAAAVTAAVYGNRAYEDALVELNDA
                     LEEQGFEPFASVAVLAEHSMVREVAAGRPDAKDHEELSGFGAQILKKLEAAEITRPQV
                     PGNRPYKEWKKMPAVPVNGSGCTGCGLCAGLCPSGAISKENPSVTDPEKCILCLRCIA
                     VCPSGAKKLPERVQEMLNQKLLPLKGIRKENELFL"
     gene            complement(7829..>8853)
                     /locus_tag="ACZPCG_09725"
     CDS             complement(7829..>8853)
                     /locus_tag="ACZPCG_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007713106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HIR1285370.1"
                     /translation="VKADGTALGTVGGGAVEYQVTLACREAVKSKESSLREFTLTRGK
                     EADIGMVCGGDVTVYIQYVDPEMPGIQELIDQVLSVLDVDEDSWLIFDLTVEKSWKMG
                     LYSEKTGVLGLGNETAGAGWMKHSCPSGAAEMKDGRDASVCERVLPETVFGPEFTALF
                     GSQAVISRENDHFYYVEPLVQSGTAYVFGGGHVAQELVPVLKKVGFRCVIMDDREMFA
                     NRQVFPDADQVIVGDMERIGDYVSIRPQDYVCVMTRGHQFDYYVQKQALALKPCYLGV
                     MGSKNKIRVVTERLLEDGFSNEEIKACHMPIGLGIHAETPQEIAVSVAAEMISVRAER
                     LGKTRR"
BASE COUNT         2088 a         2634 c         1805 g         2326 t
ORIGIN      
        1 tacacgcaga ggatcagatc tgtatctccc ggtatcaccg gctctgccgg tcccggcacc
       61 ttcaccggaa gcctctttgc tccatcggcc tccactaaga cgagatccgc tttttctctg
      121 gcctgctggt aaaattccca gccccagaag gatatctttt tctctccggg ctttcgttcc
      181 tccggatttc ccgcaaccgc aatcccactg tcttttaatg tcttctccaa agcccccacc
      241 gaaggttccg gcacaaaaaa atgctccgga agggccatat gggttgtagg aatcacaaga
      301 accttaagtc ccagttcccg cccttcccat gccatacggc ggatcaggga ggtttttcct
      361 cctccgccgg tgaatgagat cacaaacgga cagccggata accggtattt ttctatttcc
      421 agcgccttga aaatactgtg aacggcctgt gtcaaacatg ctccgtaata atatgcttcc
      481 acgttctgtc ccccatcttc tcccattgtt ctgttttatt ttatcatgga gcaaatgacc
      541 gcacaaggaa tttttatgca tctaccgcat ccacagacgc aaaacagctg tatataaaaa
      601 tagcccgatt cctgcaggca gaaccctctg cccccaaaaa ccggactgct tccttttcaa
      661 aatgtatgtt tcatcagaca ttctgcattt ctatctcaat tgtttccatg actgcggatc
      721 acacggtaca tatcatagcc aaggcttgca aatacgatta ctacggcaat aaatcccatt
      781 ttctttcttc ctctcctgtt tcttatgatg gaattattat agcccaagaa ggatataacc
      841 gcaatctgca ttgttgacaa aaatttttag tcaatctaaa actttttgta ttaaataatt
      901 ttctttttgg aatgaaccat tttcctgtaa aatttctatg gacattttac cggatttgtt
      961 atatgatact tttagttagt atatactaac ctatatattt ccaaatacat acctgcaagg
     1021 gaggatattt catgagtgtt gttatcatcg gaggtcatga ccggatggtc acccagtata
     1081 aaaaaatatg caaaacctac aactgcaaag caaaggtttt tacccagatg agtgccagcc
     1141 tggacaagca gatcggaaca ccggatctgc tcgtactctt tacgaatacg gtatcccaca
     1201 aaatgatccg ctgtgctctg gatgaggtcc gtccggacac aacccagatc gtgcggagcc
     1261 atacaagcag tgcatctgcc ttaaacgaaa ttctgtctca gcgtaccgct gcctgttctt
     1321 ctatttaaaa atgcttcagg gcatcaatgg cttcgtcgat ctgttcttct gtattaaaat
     1381 gggaaaaact gaaccgtacc gccccctgct ccacggtttt aagcgcctca tgcatcaaag
     1441 gagcacagtg acctccagag cgggtggcaa tgtcatattc ctcagaaaga tagtcgctga
     1501 cctgagcgga atcctcttct cccagattca aagatacaat agcagccctg tctttctggg
     1561 aaaaatcccc atagatacgg atatccggaa tttccagcgc ctgctcataa aaacgccgca
     1621 taagctgctg ttccctcttt ctcaaatgct ccataccctg ttccttaata tacagaagag
     1681 cggcccgcag tcctgcaatc ccatgtccgt tcaaggtacc tgcttccagc gctgtcggca
     1741 tctgtgacgg atgctgcttt tcaaaggtca taataccgct gcctccggat aaaagaggcc
     1801 gtatattcac tcctgtgcca aggcagatcc cgccagttcc ctgaggcccc atcaggccct
     1861 tgtgaccggt aaagcagagg acatcgatct gatctctctc catatcaatc ggaaagagtc
     1921 ctgcggtctg agaggcatct acaataaaaa gagcgcctgc cctgcggcac cattctccca
     1981 cctgagccag atcctggcag tttccggtga gattggaggc atgagtcata accacagcct
     2041 ttgtgcccgg agtaatggcc ttctccatgg ctatcatatc cagccttcct tttctggaaa
     2101 tcccattaga aggatccttc tcacagccca caatggtcag cacagcgccc cttttttcca
     2161 tctcatacag aggtcggagc acgctgttat gttcaagcac ggtagtcact acccggtctc
     2221 ccggatcgat caggcctttg accgccatat ttaaactttc tgtggaatta gccgtaaatg
     2281 ccacctgctg cggtccggga gcatgaaaca gctctgccag aagctgtctt gtctcaaaaa
     2341 tgatcctggc tcccgttaaa gcttctctgt gcgttccccg ggagcagttt cccatggagt
     2401 tcatcgcctc tgccactgcc cgggccacca caggaggacg gtaaaaggag gtagccgcac
     2461 tgtcaagata gatcataatg cgcctccctt aaaagccctg tttctgttcc agatataaaa
     2521 gggcttctaa tacagaaccg ccgatcgccc tcgctttatc tgaaatggta aagcagtttt
     2581 catactcgct ctctcttgga tcaatatccg cgatcttaaa gccctttgcc accggataac
     2641 cgtcccggat cagcccacgc accaggcctg taagagaagc ttctaccact acctctccct
     2701 gccccgtaac aatgaccgcg acgggctgtc ccttttctac ccggtctccg atcctcactt
     2761 ttccaaaaag gatccccatg gccggcgaat ggatgacccg ttcctttccc gcgcctccga
     2821 tcagccccgg agttcctgta ttcggaaggg ccgttcccct ggaaatgatc cttcccaggt
     2881 tgtggccccg catggtctcg atcaccagat ccacatcttc cccggctgca aagcctggcc
     2941 ccaggccaat agtcttatcc gccatattcc ggttcgttcc taggttcttt tttgccagga
     3001 tcgcatccac cagagcccag ggacggactt cctttaatat ttcacacgtc tcatccatca
     3061 tcatggggat ggctcctgcc tctaagacag cctttgcctc tgcaaaatcg gataccttta
     3121 agcaggtcac accctcaatt tcactctgtc cgtcatagat tgcttcggaa aatgccacct
     3181 gcctgcggat ggcagaagga ctgcctgttt ccagcaccag caccggataa ccgcaccgat
     3241 gaaggcggtg gatggttcct gtggcaatat cccctccgcc tcttactatg atcagctgct
     3301 tttgtctgtt catgctcccc accttaaggc cgtaccagca cagctgcccc ggtcatcctt
     3361 tctgcaatct catacatatt ggtaacgctg cctactctca gccggtcccc gatcccataa
     3421 tggtttaagc aggtgccgca ggtaagaatt tctacgccct gagcctccag cgtcttaaga
     3481 tcctccagat tatccgagcc ctcacaggaa aggaaggcgc ctccattata gagaagaatg
     3541 gtttccggca gtacgtcctg ctgtgtaaga gcataaataa atcccttcat cagaagggtt
     3601 cccagcgccg gatctccctg ccccatttca cgggaagaaa tcacaaccac cattcccttc
     3661 ctgcgtccgt ccggatggca gacttcaggc tctttttccg gtaaagcatc tgtctgcggt
     3721 gcgctttctc cttcttttac tccctcacca gctgtaataa cagccacata ttctctgctg
     3781 ctcttttttt cagatgcaaa ggacagcccc ttgtgatctg ccagcttctt taaattctgt
     3841 actgcgatct cattatccac gaccacctct actgcttccc ccggcgccgc tgcctccagg
     3901 gcccgtttcg cctcaatcac cggcagcgga cactgctttc ccctttcatc cagtcttatc
     3961 atagcctgtt tcctcctcac ttctctttta ttctacaatc acggcctttt cctgccgcgc
     4021 ttcgatcctt cccacgatcc cgcagggaag ccccagttct tccagttcct taagtgctgc
     4081 ttcggcatct gcagcatcaa tggatgcaag cagtccgccg gaggtctggg gatcaaacag
     4141 gatttcctgc atggaaaagc cacagcttcc aaaatcaatc ttatcctgca catggttgcg
     4201 gtttctctga gccgctgctg tgatatagaa ttcctctgcc aatgcaggcg cctccggaat
     4261 aaccggaatg cgatccttat agatgcgggc tgtggcctga tccgttacca tttcacacag
     4321 atgccctaaa aagctgaagc ctgttacatc cgtacatccg tggagcctgt attttttaag
     4381 aatctcggat gcatatttat taagggttgt catggactgg accgccaggt catacgccgc
     4441 ctgaggtgcc gccttcatcc ttgctgccgt gcatacgatc cctacaccca gaggctttgt
     4501 gaggatcagg gcatcgccct ctttgcagtc gctgttggca aatacacggt caggatggat
     4561 aattcccgta acagaaaggc cgtattttac atcgctgtcc gcaatggaat ggccgcccgc
     4621 cagaacacct cctgcctctc gtaccttttc gctgccgccc tgcatgatct ttcccagaat
     4681 attcagatcc atctcttccg gaaagcatac gatatttaat gcagttttta cctctcctcc
     4741 catggcatag atatcactca gcgcattggc agccgcaatc tggccgaata tatagggatc
     4801 ctccaccatg ggaggaaaaa aatcaagtgt ctgcaccatg gccagatcat ctgtaagccg
     4861 gtatacggcc gcatcatcgg aaccgtcata tccgatcatc agattgggat cctttgtttt
     4921 gggcagccga tttaacacac gggccagtac ccccggcccc agcttggccg tacagcctcc
     4981 tcccctgcaa aatacgattt cctcactcat ttttttcatt tcctcccatc tctctcatat
     5041 gatttaacca ttcatccgaa aaatacgttt caaacagacg gtccagttcc gcttctgcat
     5101 ctcttcgaaa gcttaaataa gcttccatca gctccttcgc ctgaggcgta agggtggaac
     5161 ctcctccgtc tcttcctccg gtctcccggt ctgtcagcaa aaacccccac tccttttccg
     5221 ccacccggag cattttccat gccttactgt aggccatatt catagagcgg gccgctctct
     5281 gcagagatcc tgttttctct accgctctca gcaaagccac catccccgga ccaaaggcct
     5341 tttcctcaaa atacaggcgc aatgataaac cgtaatgaag cttattgtta tcttccatct
     5401 tctgccctcc cgttatactc ttttgtatat aacgatttct acaaaaaaac tggtctctgt
     5461 ctttttggat ccagccataa aagacgcagc cagtattttg tgtgctatgt gtaatagtat
     5521 accatgcttt ctcacgcttt tccagccctt tcatatatat aaattacaga cagaaataag
     5581 gcaggaacag ccatgagaac attttttgca gtcgggggcg atatgcgtct tcgatggatg
     5641 gtacagattc tttccaagga aggatatccc gtgtcttttt ttacaggaac ggattccccg
     5701 ctgatgaaat cggcagtcaa agaggcctct gtgatcctcg gccccgttcc ctttacccgc
     5761 gacggggttc atctgttcca gcctccctca cacggtatgg aactctctgc ccttctttcc
     5821 tgccttatgc ccggacagta tctttttggc ggaaatctcc cggatcctgt aaaagagttc
     5881 tgcgataata atgacattct atggaaggat tttatggaca tggaagaaat ttccctggaa
     5941 aatgccattg ccaccgcaga aggcgccatc tctctggcag tcagccagtg ccccatcaat
     6001 ctccatcgct gccgctgcct gatccttgga tatgggcgct gcggccaggc gctggctgac
     6061 cgcctgagag gaatgtttgc agatgcatcc gtctgtgaga taaatcccat ccggcagaca
     6121 cttgccaaaa cgcgtggctt tgacaccttt gatccccaaa aactggaaaa atatcttcct
     6181 caggcagagc ttatttttaa tacagttccc gctcctatcc ttacagatcc cctgcttatc
     6241 aagcagatcc gtaaagacgc ggtacttttt gaactggctt ccggctccgg ctgtatttct
     6301 gaggaagcct gccgtcaggc cggactttgc cggatcgcct gtccggggct tccgggacgt
     6361 ttcagtccca aagcctctgc caggatcctg tgccgggcag tactgcaggt ccttggagag
     6421 ggacgttaaa aactactaca tttataatag agggaaattc ttatgtctct gaaaggaaaa
     6481 acgatcggcc tggctgttac cgggtccttc tgtacctttg ataaaataaa acagaccgta
     6541 caaactctca tacaggagga agccgagctg taccccatct tttcctccca tgtgcagacc
     6601 acccgttccc gtttcggaag cacggatgaa tatatccgga ccatcagcgg tctggccgga
     6661 cgagaaccca ttcttaccat tgaggacgcg gaacccattg gccccaaagg ctatctggat
     6721 cttctgctca tcgccccctg tacgggaaat acccttgcca agctggcaaa cggcatcacg
     6781 gatacccccg tactcatggc tgccaaagcc catttgcgta atgaaaagcc tctggtgatc
     6841 ttcctttcca ccaatgatgc catgggaatg aattttaaaa atatcggact tctctttccc
     6901 tgcaaacatg tatattttgt tccattttcc caggattcac cgcaaaaaaa gccctgttcc
     6961 ctggttgcga gaacagagct tgtaattcca accataaaag ctgctctgaa gggtgaacag
     7021 ctccagcctg ttttatatgg tccggactaa ttaccccaaa ttttacaaaa acagctcatt
     7081 ttccttccgg atccccttta acggcagaag cttctggttt aacatctcct gaacacgttc
     7141 cggaagcttt tttgcaccgg aaggacagac agcgatgcag cgcaggcaca ggatacattt
     7201 ctccggatct gtcactgatg gattttcttt tgaaatagct ccagagggac agagtccggc
     7261 gcagagtccg cacccggtac agccgctgcc gtttaccggt actgcaggca tctttttcca
     7321 ttccttatat gggcggttcc ccggcacctg gggccttgta atttctgccg cttcaagctt
     7381 tttaaggatc tgtgctccaa aaccagaaag ctcctcatga tccttcgcat ccggacgtcc
     7441 tgccgccacc tcccgaacca tggaatgctc cgcaaggaca gccacagaag cgaaaggctc
     7501 aaagccctgc tcctccaatg catcatttaa ctccaccaga gcatcctcat aggccctgtt
     7561 tccataaacg gcagccgtaa cagcagcagc gccctttcct gcggtgcact ttaaccgctc
     7621 tgccatcagt gccgggatcc ttcctccaaa tgcaggagca gccacaatca ccagatcatc
     7681 ctttttaaat tctgcgccat taaaatcagg agcagaaaga tccacctctt ttacctcttt
     7741 tccaaggctc tctgccagat agaaggccgc tttttttgtt cctcctgtgg gactgaaata
     7801 tattgcccat acacattcct tcatgccttt acctccttgt ctttcccagt ctctcggccc
     7861 gcacagagat catctccgcc gcaacgctga ctgcaatctc ctggggtgtt tccgcatgga
     7921 ttcccaaacc gatcggcata tggcaggcct tgatctcctc atttgaaaat ccatcttcaa
     7981 gcagccgttc cgtcaccaca cggatcttgt ttttgcttcc catcacgccc agatagcagg
     8041 gctttaaagc cagagcctgc ttctgcacat aatagtcaaa ctgatggcct cttgtcatca
     8101 cgcatacgta gtcttgaggc cggatagaca catagtcgcc aatccgctcc atatctccca
     8161 cgattacctg atcggcatcg ggaaatacct gccggttggc aaacatctct ctgtcatcca
     8221 tgatcacaca gcgaaagccc acttttttta atacaggaac cagctcctga gccacatggc
     8281 ctccgccgaa cacatacgcc gtaccagact gaaccagagg ctccacatag taaaaatgat
     8341 cgttttctct ggaaatgacc gcctgggaac caaaaagcgc tgtaaactca ggcccaaata
     8401 ccgtctccgg caacacccgt tcacatacag aagcgtccct tccatctttc atttctgcgg
     8461 ctcccgaagg acagctatgc ttcatccacc ctgctcctgc cgtttcattt ccaaggccca
     8521 aaacgcctgt cttttcactg taaagcccca ttttccacga tttttccaca gtgaggtcaa
     8581 aaatcagcca gctgtcctca tccacatcaa gaacactcag aacctgatcg ataagctcct
     8641 ggatacctgg catttcagga tccacatact ggatgtacac cgtcacatct cccccgcaga
     8701 ccataccgat atcggcttct ttcccacggg ttaaagtaaa ctctcgaaga ctggactctt
     8761 tacttttaac tgcttccctg caggccagag ttacctgata ctccacagct cctccgccca
     8821 cagttccaag agccgttcca tctgctttta cca
//
LOCUS       DBMDUH010000114         8717 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_3684_length_8717_cov_5.110144, whole genome shotgun sequence.
ACCESSION   DBMDUH010000114 DBMDUH010000000
VERSION     DBMDUH010000114.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 8717)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8717)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8717
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3684_length_8717_cov_5.110144"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            241..918
                     /gene="deoC"
                     /locus_tag="ACZPCG_09730"
     CDS             241..918
                     /gene="deoC"
                     /locus_tag="ACZPCG_09730"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HIR1285371.1"
                     /translation="MKKELIYQHIDHTLLKAVSSWEDIHKLCEEAVAHGMASVCIPPS
                     YIKRVHEVYGDKLNICTVIGFPLGYNTTEVKRSEVEQAIAEGAKEVDMVVNLGDVKNG
                     DFDRVTEEIATLKRAAGDKILKVIIETCYLTEEEKIRLCRCVADGGADYIKTSTGFGT
                     AGAKIEDIRLFKENLPKDKDVKMKAAGGVRTREDLELFLEEGCERIGTSSAIKILEDG
                     ENNNGAY"
     gene            950..2077
                     /locus_tag="ACZPCG_09735"
     CDS             950..2077
                     /locus_tag="ACZPCG_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family lipoprotein"
                     /protein_id="HIR1285372.1"
                     /translation="MKKTMKKIGAALLAAMMVMSVTGCSQGGNTAGTSAAGEGASGAG
                     AEIAMIADGGSIDDQGFNQGTYEGVKQYGEEKGISYKYYKPVEKTTDAYLSAMELAIE
                     GGAKIIVAPGFRFTEAMYVAQDTWPDLKFVLLDGVPSSNGDTKIGENTVGIQYAEEQA
                     GFLAGYAAVVEGYKKLGFMGGVATPAVVRYGYGFIQGAEAAAKEMGVSDVQMNYYYTG
                     TFDATPETQTLAATWYQNGTEIIFACGSGVGQSVMAAADASASGKSIGVDIDWAGESE
                     TVVTSAMKSLSGTVYQMLEKFYSDSFPGGENIMMDASNKGVELPMETSRFETFDQAKY
                     DEVYGKLADGTFKPLRDVNEEGTSYTLDQISDGTIEVTEVK"
     gene            2114..3634
                     /locus_tag="ACZPCG_09740"
     CDS             2114..3634
                     /locus_tag="ACZPCG_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1285373.1"
                     /translation="MEYVIEMLGIRKEFPGIVANDNVTLQLKRGEIHALLGENGAGKS
                     TLMSVLFGMYQPEAGEIRKNGQVVHIKNPNDANKLGIGMVHQHFKLVHNFTVLENVIL
                     GVETTQKGLLKMDEARKKVVSLSEKYGLKIDPDALISDITVGMQQRVEILKMLYRDNE
                     ILIFDEPTAVLTPQEIDELIKVMKNLAAEGKSILFITHKLNEIKEAANRCTVLRKGRY
                     VGTIDVASTTKEEMSEMMVGRKVNLQIDKGGYNPGECVLAVEDLSVKNHFTGKLSVNH
                     VSFDVKAGEIVCIAGIDGNGQSELVYALTGLTPTESGSIHLKGQDFTHMSIRERNTHG
                     LGHIPEDRHKHGLVLDYDLAYNTVLQSYFEKKFQNHGFLKEREIYSYADGLIQSFDIR
                     CGQGGKTISRSMSGGNQQKIIIAREIDRNPDLLIAVQPTRGLDVGAIEYIHKQLVAQK
                     KAGKGVLLVSLELDEVMSISDRILVMYEGEIVADLKPEDVTVQELGLYMAGSKRRA"
     gene            3689..4741
                     /locus_tag="ACZPCG_09745"
     CDS             3689..4741
                     /locus_tag="ACZPCG_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1285374.1"
                     /translation="MAILLGMLVGLIILVASNPAQAGQGFTAILLGGLSNLKNMGQVL
                     YYATPLIMTGLSVGFASKTGLFNIGASGQFIVGAFAAVFVGVRFTFLPGALHWIVALL
                     AAILAGALWGMVPGILKATRNVNVVIGCIMMNYIGMYSVNALVSAFIYDPLQSQSLPV
                     DADAILPKMGMDMIFRYNNRPSSANAGILIAILSGVAIWVILQKTVFGYELKACGYNS
                     EAGRYAGMNEKKNVISSMAIAGALAGLGGGLLYLAGSGNGIQVVDTLAAEGFNGIPVA
                     LLAMNNPIGIIFSGIFIAYLKVGGDQMQLYNFVPEVIDIIISVIIYFSAFALVFRGVV
                     SKVFSGKKTDTADKTA"
     gene            4755..5708
                     /locus_tag="ACZPCG_09750"
     CDS             4755..5708
                     /locus_tag="ACZPCG_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1285375.1"
                     /translation="MSLLYFLVQQMMLFSIPLLIVALGGMFCERSGVINIALEGIMVL
                     GGFAGILFINLFQGVLPGQPILLIALLISMVTGMVVSLVHAYASVNMKANQSISGTAI
                     NMFAPAFAIFVARVIRNVQQIPFENQFRIEKVPVLGDIPVIGGMFFQNAYITTYLGIL
                     ILVVSCVVLYKTRFGLRLRSCGEHPQAAASVGISVYKMRYMGVLISGALAGIGGLVYI
                     IPTSTSFNASVSGYGFLALAVLIFGQWKPGKILLAALFFGIMKTISSAYSGIPILADF
                     GIPSSIYKMIPYLATLIVLALNTKGSQAPKASGVPYDKGAR"
     gene            5745..6491
                     /locus_tag="ACZPCG_09755"
     CDS             5745..6491
                     /locus_tag="ACZPCG_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006779818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HIR1285376.1"
                     /translation="MPFLNEEGKQYHINCKEGDVGRYVLLPGDPFRTDVIASMFDDAK
                     LVAHNRECKTWTGTLNGEKVSVTSTGMGSPSTAIVVEELIRCGADTFIRVGTSGRVCP
                     ASQDMSLNGVINTAAVRDEGTTISYVPLAYPAVADRHVVDALARAAKKLGYNFAEGIT
                     HSKDAFYAEIEPDNMPNSDYVKSRWQAWERSNVMCSEMEAAAIFIVSSIRNCRASAVM
                     AYKKVNLQAAMETAIEGLRILIEEDKKLGR"
     gene            6494..7936
                     /locus_tag="ACZPCG_09760"
     CDS             6494..7936
                     /locus_tag="ACZPCG_09760"
                     /inference="COORDINATES: protein motif:HMM:NF014081.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR1285377.1"
                     /translation="MERLLIKNIRYLYCMDEEDTFLENADIYIEGNVIKKIGTKLDVP
                     ADGVKVIDGTGKLALPGFINTHHHFYQNITRNIPIMQKGNLLGWLLYSYGAWENMEEE
                     DVKAAARLATAELLMTGCTTSMDFMYFFPHGKHDLMDAEFEAVKELGLRFHGFRGCMP
                     VMEGNLPAEMKERLGIDAGSLVESYDDILDSCDRTFQKYHDDSRFSMSRVGVGPTTVV
                     FENPEFMKELKKMADNRGGLCHTHLHPRPDEIKKCGELYNCRPHQWLEKIGWIDKNVS
                     FAHISRHNAEELDIVARNGASVTQSPSCHMRLGYPIAPALEARDRGIPVSIGVDGGAS
                     NDSGDMLGELRTMLYVHRIDGGHPDYGPERWINAKEVFEMATVNGAKCLNRDDIGMLK
                     EGMAADLVLFSMIQPGYAGALTDPRGALLYCGNNHLADTTIVNGNVLIENGIFLAGDL
                     NQIVEDANRSTARILKLVKDKTGIDYRMEA"
     gene            7942..8697
                     /locus_tag="ACZPCG_09765"
     CDS             7942..8697
                     /locus_tag="ACZPCG_09765"
                     /inference="COORDINATES: protein motif:HMM:NF036686.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maleate cis-trans isomerase family protein"
                     /protein_id="HIR1285378.1"
                     /translation="METGRNRTETVRFPKRIGMIVPSSNTVVEPICSRIFFGMEDVVT
                     VHYSRLEVVNVSLDDNSTRQFAVDTMLRAADQLAQAKVDAIIWNGTAGSWLGIERDRE
                     MCQKIEEKTKIPMSTASLGLVGSYQDFDIKRLHLVTPYIKEMNDAIIREYEKLGLTAA
                     GVTGLGITENTEIGSVTKEQLETLCCSTGAGHGEGIAVVCTNLAAAWRADLIEKRSGA
                     VVFDSVTAAVREALKLCGLEKLSFQGWGKLLDV"
BASE COUNT         2519 a         1522 c         2351 g         2325 t
ORIGIN      
        1 aatgggtgtt acaattgtaa caacttaata aataaaacat ttaacgttga tgagctcaaa
       61 aagccagccg tttcggttgg cttttttgtt ttttgtgcac aaagcgttga gtgtagaatt
      121 tatcagattc agaaaagtgt ctgccggcaa atcacttttt tgaataaaat gttttcgaaa
      181 acaaagcgca accagaaaaa tgtagattct gcagatgcag aggaaagagc aaatgaactt
      241 atgaaaaaag agcttattta ccagcatatt gatcacacat tattgaaggc ggtttcttcc
      301 tgggaagata ttcataaatt atgtgaagag gcagttgcgc atggaatggc atctgtctgt
      361 attccgccat cctatatcaa gagagtacat gaggtatatg gtgataagct gaatatctgt
      421 acagtaattg gtttccccct gggatacaat accacagaag tgaaacggtc agaagtggag
      481 caggcaattg cagagggagc caaagaagta gacatggttg taaatctggg agatgtaaaa
      541 aatggagatt ttgaccgtgt aacagaagaa attgctactc tgaaacgtgc agcaggagac
      601 aagatcttaa aggtaattat tgaaacctgt tatctgacag aagaagaaaa gattcgtctt
      661 tgcaggtgtg tggcagacgg aggagcagat tatatcaaga catctaccgg atttggtact
      721 gcaggagcaa agattgaaga tatccgtctt tttaaagaga atcttccaaa agataaggat
      781 gtaaagatga aggcagccgg aggcgtgaga accagagaag atctggaact gtttttagaa
      841 gagggatgcg agcggattgg aaccagttct gcaattaaga tactggaaga cggcgaaaat
      901 aataacgggg cttactaatt agagttcaga ttgagaaagg agaaggatca tgaaaaaaac
      961 gatgaaaaaa ataggtgcag cattattagc agcgatgatg gttatgtcag taacaggatg
     1021 ttctcagggc ggtaatacag caggtacttc agctgcagga gaaggtgctt ccggtgcggg
     1081 tgctgagatt gcgatgatcg cagatggcgg aagcattgat gaccagggat ttaaccaggg
     1141 aacatatgaa ggcgtaaaac agtatggtga ggaaaaagga atttcctaca aatattataa
     1201 accggtagaa aagacaacag atgcatattt gtcagccatg gaattggcaa tcgaaggcgg
     1261 agcaaaaatt attgtagcac ccggctttcg gtttacggag gcaatgtatg tagcgcagga
     1321 tacatggccg gatcttaaat ttgttcttct ggatggagtt ccttccagca atggagatac
     1381 aaagattggt gaaaatacag taggtattca gtatgcagag gaacaggccg gttttctggc
     1441 cggctatgca gcggttgtag aagggtacaa aaaacttggc tttatgggag gcgttgcaac
     1501 tccggcagtt gtacgttacg gatacggttt tatccaggga gctgaggctg ctgcaaagga
     1561 aatgggtgtg agtgatgtac agatgaatta ctattataca ggtacatttg atgctactcc
     1621 agaaactcag acactggcag caacctggta tcagaatgga acagagatta tttttgcatg
     1681 tggatccggt gttggtcagt ctgttatggc agcagctgat gcttctgcat ccggcaagtc
     1741 tattggcgta gatattgact gggcaggaga atctgagaca gttgttactt ctgctatgaa
     1801 atctttaagc ggtacagtat atcagatgct ggagaagttc tacagcgaca gtttcccggg
     1861 cggagagaac attatgatgg acgcatccaa caagggtgtg gaacttccga tggaaacctc
     1921 cagatttgaa acgtttgatc aggctaagta tgatgaggtt tatggaaaac tggcagatgg
     1981 cacatttaag ccgctccgcg atgtaaatga agagggaact tcctatacac tggatcagat
     2041 ttctgacgga acaatcgagg taaccgaagt aaaataaaga gtattcagct agtgggatgc
     2101 cgggagaatg aatatggaat atgtaattga aatgttggga atccgaaagg aattcccagg
     2161 aattgttgca aatgataatg taacacttca gctaaaacgt ggtgaaattc atgcattact
     2221 gggagagaac ggtgcaggaa aatcaacatt gatgagcgta ctgtttggta tgtatcagcc
     2281 agaagccggt gagatacgca agaatggaca ggttgtacat ataaagaatc ccaacgacgc
     2341 aaataagctg ggaattggta tggttcatca gcattttaag ctggttcata actttacggt
     2401 tctggagaat gtaattctgg gtgtggaaac cacccagaag gggcttctga aaatggatga
     2461 ggcccgcaaa aaggtagtaa gcctgtcgga aaaatatggg ctgaagattg atccagacgc
     2521 attgatttct gatattacag taggtatgca gcagagagta gagattttaa agatgctgta
     2581 tcgtgataat gaaatattga tttttgatga accaaccgct gttctgactc ctcaggagat
     2641 tgatgagctg atcaaggtta tgaagaatct ggcagcagaa ggcaagtcga tcctgtttat
     2701 tacccataaa ctcaatgaga taaaagaggc cgctaaccgc tgtacagtac tgcgaaaagg
     2761 aagatatgta ggtacgattg acgttgcttc caccaccaaa gaagaaatgt ctgaaatgat
     2821 ggtgggaaga aaagtaaatt tacagattga taaaggaggc tataatccgg gtgaatgtgt
     2881 gctggctgta gaagacttaa gcgtaaagaa tcattttaca ggaaagctgt ctgtcaatca
     2941 tgtatcgttt gatgtaaagg caggagaaat tgtctgtatt gcaggaattg acggaaacgg
     3001 acaaagtgaa cttgtatatg ctcttacagg actgacaccg actgagagcg gatcgattca
     3061 cttgaaaggt caggatttta ctcacatgag tatcagagag cgcaatactc atggacttgg
     3121 acatatcccg gaagaccgtc ataaacacgg attggttctg gactatgatc tggcatataa
     3181 tacggtactc cagagttatt ttgaaaaaaa gttccaaaat catggtttct taaaagagcg
     3241 ggagatttac agctatgcag acggactgat tcagtcgttt gatatccgct gcggacaggg
     3301 aggtaagacc atctccagaa gtatgtccgg aggcaatcag cagaagatca ttattgcaag
     3361 agagattgac cgtaatccgg atcttttaat cgctgtgcag cctacaagag gtctggatgt
     3421 aggtgcgatc gaatacatcc ataagcagtt agtcgcgcag aagaaggcgg gaaaaggggt
     3481 tcttctggta tccctggaac tggatgaggt tatgagtatc agtgaccgta ttctggtaat
     3541 gtatgaaggt gagatcgttg ccgatttaaa accggaggat gtaacggtac aggaactggg
     3601 actctatatg gcagggtcca aaaggagggc atagaacgtg aaaagaaaat ctcttccatc
     3661 cggcgtttcc agttttctgg cgtctgttat ggcaattctt ctgggaatgc ttgtcggatt
     3721 gattatttta gtagcaagta atccggcaca ggcaggtcag ggctttacag caattcttct
     3781 gggcggtctg tcaaacttaa agaacatggg gcaggtactg tactatgcaa cacctttgat
     3841 catgacaggt ttatctgtgg gatttgccag taagaccgga cttttcaata tcggagcatc
     3901 cggtcagttt atcgtcgggg cattcgcagc agtgtttgta ggtgtacgtt ttacattttt
     3961 accgggtgct cttcactgga tcgtagctct tttagcagcg attctggctg gtgctttatg
     4021 gggaatggtt cctggtattt taaaagcaac aagaaacgta aatgtggtaa ttggatgtat
     4081 catgatgaat tatattggaa tgtattctgt gaatgctctg gtgagtgcgt tcatttatga
     4141 tccgctgcag agtcagtctc ttccggtaga cgcagatgca attcttccaa agatgggaat
     4201 ggatatgatc ttccgctata ataaccgtcc atccagtgct aatgcgggaa ttctgattgc
     4261 aattctgtca ggtgttgcaa tttgggtcat cctccaaaag actgtgtttg gttatgaatt
     4321 gaaagcctgc ggctataaca gtgaagcagg ccgttatgca ggtatgaatg aaaagaaaaa
     4381 tgtgatttct tctatggcaa ttgcaggtgc tctggctggt ctgggcggcg gtttgcttta
     4441 ccttgcaggt tccggaaacg gaattcaggt agtagatacg ctggcagcag aaggctttaa
     4501 cggtattccg gtagcgctgc ttgctatgaa taatccaatc ggtattatct tctccggtat
     4561 ttttatcgca tacttaaagg tgggcggaga tcagatgcag ctgtataatt tcgttcctga
     4621 ggttatcgat attattattt ctgtaattat ctatttcagt gcatttgcgt tggtattcag
     4681 gggtgtagtc agcaaggtat tctcggggaa aaagacggat acggcagata agacggcgtg
     4741 aaaggagaag tgacatgagt ttattatatt ttttggttca acaaatgatg cttttttcca
     4801 tcccgctgct gatcgttgca ctgggaggaa tgttctgtga gcgaagcggt gttatcaaca
     4861 ttgctctgga gggcatcatg gtgctgggcg gatttgccgg gattctgttt atcaatctgt
     4921 ttcaaggtgt acttcccgga cagccgattt tgctgattgc actgctgatt tctatggtga
     4981 ccggaatggt ggtatctctg gttcatgcat atgcctctgt aaatatgaag gcaaatcaga
     5041 gtatcagcgg tacagcaatc aatatgttcg ctccggcatt tgctattttt gttgccagag
     5101 taatccgcaa tgtgcagcag attccgtttg agaatcagtt ccgaattgaa aaagtaccgg
     5161 ttttgggaga tattccggtg ataggaggaa tgtttttcca gaatgcctat attactactt
     5221 atctgggaat tctgattctg gtggtttcct gtgtagttct gtataaaaca agatttggac
     5281 ttcgtctgcg ttcctgtggt gaacatccgc aggcagcagc atctgtggga atcagcgttt
     5341 ataaaatgcg ttatatgggt gtactgattt caggcgcgtt agctggtatt ggcggactgg
     5401 tatacatcat tcctacttct accagtttca atgcatctgt atcaggatat ggattcctgg
     5461 ccctggctgt tttgatcttc ggtcagtgga aacctggaaa gatcctttta gcagctctgt
     5521 tttttggtat catgaagacc atatcttctg catactcagg aattccgatt cttgcagatt
     5581 ttggcattcc aagcagtatt tataaaatga ttccatattt ggcaaccctg attgttctgg
     5641 cactcaatac gaagggatcc caggcgccaa aggcatccgg tgttccttat gataaaggtg
     5701 cgagataaca cagactgtat ttatatgaac aggaggataa taatatgccg tttttaaatg
     5761 aggaaggaaa acagtatcat ataaattgta aggaagggga tgtgggtcgt tatgtgctgc
     5821 ttcctggaga tccattccgt acagatgtga tcgcatctat gtttgatgat gcaaaactgg
     5881 tggctcacaa cagggagtgt aaaacctgga ctggaacatt gaacggagaa aaggtatctg
     5941 ttacttctac tggtatgggc agcccgtcta cagcaatcgt agtagaagaa ctgattcgtt
     6001 gcggtgcaga tacgtttatt cgcgtgggaa cttccgggcg tgtgtgtccc gcctcccagg
     6061 atatgtcttt aaatggtgtg atcaacactg cagcagtacg ggatgaaggg acaacgattt
     6121 cttacgttcc tctggcttat ccggcagtgg ctgaccgtca tgtagtagat gcactggcca
     6181 gagcagctaa aaagctgggt tataattttg cagagggtat tacccattcg aaagatgcat
     6241 tttatgcgga gattgagccg gataatatgc ccaacagcga ctatgtaaaa tcccgctggc
     6301 aggcgtggga acgctcgaat gtaatgtgtt ctgagatgga ggctgctgcg attttcatcg
     6361 tttcttccat cagaaactgc cgtgcatctg ccgttatggc gtacaagaaa gtaaacctcc
     6421 aggctgcgat ggaaacagca attgagggtc tgcggatttt aattgaagaa gacaagaagc
     6481 tggggaggta acgatggaac ggttgctgat taaaaatatt cggtaccttt attgtatgga
     6541 tgaagaggat acatttctgg agaatgcaga catttatata gaaggaaatg tgatcaaaaa
     6601 aatcgggacg aagctggatg tgcctgctga tggggtaaaa gtgatagacg gaactgggaa
     6661 gctggcactt cctggattta tcaataccca tcatcatttt tatcagaata ttaccagaaa
     6721 tattccgatc atgcagaagg gaaatctttt gggctggctt ctttactcat acggagcatg
     6781 ggaaaatatg gaagaagagg acgtaaaggc ggcagcacgt ctggctacag cagagctcct
     6841 gatgacagga tgtaccacat ctatggactt tatgtatttc ttcccccatg gaaaacatga
     6901 tttaatggat gcagaatttg aggccgtaaa agagctggga ctgcggtttc acggattccg
     6961 tggctgtatg cctgtaatgg agggaaacct gcctgcagaa atgaaggaac ggctggggat
     7021 tgatgccggg agtctggtgg agtcctatga tgatattctg gattcatgtg acagaacatt
     7081 tcaaaaatac catgatgaca gccgtttttc catgagccgc gtaggcgtag gccctactac
     7141 tgtggtattt gagaatccgg aatttatgaa agaattgaag aagatggcag ataatcgcgg
     7201 cggtttgtgt catactcatc ttcatccaag accagatgag atcaaaaagt gtggagagct
     7261 gtataattgc agacctcatc agtggttgga gaaaatcggc tggatcgata aaaatgtatc
     7321 ttttgcccat atcagccgtc ataatgcaga agaactggat attgtagcga gaaacggcgc
     7381 gtctgtgacc cagtcgccgt cctgtcatat gagactggga tacccgattg ctccggcact
     7441 ggaagcgaga gaccggggga ttcccgtaag tatcggtgta gacggcggag ccagcaacga
     7501 cagcggagat atgctgggag agctgagaac catgctttat gttcaccgga ttgacggcgg
     7561 acatccagac tatggaccgg agcggtggat caatgcaaaa gaagtatttg agatggcaac
     7621 ggtaaacggt gcaaaatgtc tgaacaggga tgacattggt atgctgaaag agggaatggc
     7681 agccgatctt gtactgtttt ccatgataca gccggggtat gccggagcat tgacagatcc
     7741 aaggggagca ctgctgtact gtggaaataa tcatctggca gacacaacca tcgtgaatgg
     7801 aaatgtgtta atcgagaatg gcatattcct ggcaggagat ttaaatcaga ttgtagagga
     7861 tgccaatcgt tcaacagcaa ggatattaaa gcttgtgaag gataagacag gcattgacta
     7921 tcgtatggag gcataggcat catggagaca ggcagaaaca gaacagagac agtgcggttt
     7981 cctaagcgga ttggtatgat cgttccttcc tctaatactg tagtagaacc aatatgcagc
     8041 cgtatttttt tcggtatgga ggatgtagtt accgttcatt attccagact ggaggtcgta
     8101 aatgtttctc tggatgataa ctctaccaga caatttgctg tggatacgat gcttcgggca
     8161 gccgatcagc ttgctcaggc caaggtagat gcgattatct ggaacggaac ggcaggaagc
     8221 tggctgggaa ttgagcgtga cagagagatg tgccagaaga ttgaagaaaa aaccaagatt
     8281 cccatgagca cagcgtcttt ggggctggta ggaagttatc aggattttga tataaagcgt
     8341 ctgcatcttg tgacacctta tattaaagaa atgaatgacg caattattcg ggagtatgag
     8401 aagctggggc ttacagcagc aggagtgacc ggccttggaa tcacggaaaa tacagagata
     8461 ggcagtgtca cgaaggaaca gctggagacc ctgtgctgca gcacaggcgc aggccatggc
     8521 gaggggattg cagttgtctg tacgaacctg gctgctgcct ggagagcgga cttgatcgaa
     8581 aaacggtcag gagcggttgt gtttgattcc gtaaccgcag ctgttcggga ggcattgaag
     8641 ttgtgtgggt tagaaaaatt gtccttccag ggatggggaa agctgctgga cgtataagct
     8701 aagatggagg aaacaac
//
LOCUS       DBMDUH010000115         8642 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_3721_length_8642_cov_5.165437, whole genome shotgun sequence.
ACCESSION   DBMDUH010000115 DBMDUH010000000
VERSION     DBMDUH010000115.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 8642)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8642)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8642
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3721_length_8642_cov_5.165437"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="ACZPCG_09770"
     CDS             <1..434
                     /locus_tag="ACZPCG_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009267652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HIR1285379.1"
                     /translation="MLYGGIPLVVLREGANDKAAALQNLFNEIYLRDITKRNRVKNIG
                     ELEDLLNILSSAIGSLTNPEKLKNTFKTIKKSRITSATIKKYLDYFEDSFLIESAQRY
                     DIKGKAYIETPKKSERTRGRTRTRSGRLRSGTSFMMTTGSL"
     gene            480..620
                     /locus_tag="ACZPCG_09775"
     CDS             480..620
                     /locus_tag="ACZPCG_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285380.1"
                     /translation="MSMTRPGTAPSQRKRSFTAIWSPGRSEINGVKYSDIDYINRILK
                     VE"
     gene            1154..3151
                     /gene="uvrB"
                     /locus_tag="ACZPCG_09780"
     CDS             1154..3151
                     /gene="uvrB"
                     /locus_tag="ACZPCG_09780"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HIR1285381.1"
                     /translation="MQNEQKIFKLHSEYAPTGDQPQAIEALVKGFQEGNQFQTLLGVT
                     GSGKTFTMANVIAQLNKPTLVIAHNKTLAAQLYGEFKEYFPGNAVEYFVSYYDYYQPE
                     AYVPSTDTYIEKDSAINDEIDKLRHSATAALSERSDVIIVASVSCIYGLGSPIDYQEM
                     VISLRPGMEKDRDDVIRKLIEIQYDRNDMDFKRGSFRVKGDVLDIYPAYSDGQAFRVE
                     FFGDEVDRIMEIDTVTGEVKAQLGHVAIFPASHYVVPKEKMMEATEHILEELKERVSF
                     FKSEDKLLEAQRISERTNFDVEMMRETGFCSGIENYSRHLTGTAPGEPPCTLIDYFPK
                     DFLIIVDESHITLPQVRGMYAGDRSRKMTLVDYGFRLPSALDNRPLNFSEFESKIDQM
                     LFVSATPSVYEQEHELMRVEQIIRPTGLLDPEIFVRPVEGQIDDLISEVNKEVAGHHK
                     VLITTLTKRMAEDLTDYMREVGIRVKYLHSDIDTLERAQIIRDMRLDVFDVLVGINLL
                     REGLDIPEITLVAILDADKEGFLRSETSLIQTIGRAARNSEGHVIMYADSITDSMRVA
                     IEETKRRRQIQQRYNEEHGITPTTIKKAVRDLITISKAVENDSFADTKDPESMDEKEL
                     KKLSKELEKKMRQAAAELNFEEAARLRDRMVEVKKLLLDME"
     gene            3289..4470
                     /locus_tag="ACZPCG_09785"
     CDS             3289..4470
                     /locus_tag="ACZPCG_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HIR1285382.1"
                     /translation="MYTKKMFSLAMAAFLSAMAVFPVYAGTEKIDTVRLEFSYDKEPE
                     SGDDIGDISVRARDDSYDVDSVEYTNLEDKDVWTVGDVPEVKVELSASEGYRFSYTSK
                     SHFKISGCDAEFKKAKIYDDGDYMEVTVELDRIGGKLEAADNLDWNDSTAEWDEVEGS
                     KSYDVKLLRDDKTVTTVNTSNTYFDFSGYFNKEGDYTFRVRAIASYNDKAGEWSEDSS
                     SLYVDEDEAGSYGGSGRWVQEGGRWWYSYSTGGYPADGWKQIDGVWYLFDRSGYMLTG
                     WQKVDRQWYYLGSNGALTTGWQNVNGQWYYLNGSGEMQTGWQYVSGRWYYLNSSGVMQ
                     TGWLEQNGQWYYLDGSGAMTTGWQSVNGRWYYMGGSGVMFRNTWTPDGRYVDGSGAWV
                     Q"
     gene            complement(4550..5461)
                     /locus_tag="ACZPCG_09790"
     CDS             complement(4550..5461)
                     /locus_tag="ACZPCG_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5688 family protein"
                     /protein_id="HIR1285383.1"
                     /translation="MVYDVFLNKIKTGMEQALGKEYELSLHRITKNNGLILDGLCISK
                     DKNPVAPAIYLNNCYHQYLEGCSLEEITQELLELYRSNEALPTIHPARLSDYSAMKSR
                     TACRLINTAANETLLQDMPHIPWNDLSIVFYLFLQENDTGIMTAGIHTSHLNTWGISQ
                     DELFQAAISNVSQLFPPSITSMDCILEDLTDSVPDLNRQAMTPFYVLSNTCGINGAIC
                     MLYPDVLKHFSEGIERDVLILPSSIHEVLLLPDEEDISYDELSQMVAQINASEVLPQD
                     RLSNQIYRYSRRSDSIEIVSSCMLPLN"
     gene            5825..6871
                     /gene="hrcA"
                     /locus_tag="ACZPCG_09795"
     CDS             5825..6871
                     /gene="hrcA"
                     /locus_tag="ACZPCG_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003498602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HIR1285384.1"
                     /translation="MDTQLDNRKITILKAIIKTYLETGEPVGSRTISKYSELKLSSAT
                     IRNEMSDLEEMGYIIQPHTSAGRIPSDKGYRFYVDQIMQEKDNEVTEFKDMMVQKVDK
                     LELVLKKMAQVLAANTNYAAMISGPSYHKTKLKFIQLSRVEAHKLLVVVVVEGNIVKN
                     TMIDIAEELGEQELLNLNILLNSSLNGLTIEEINLDIISRLKEDAGIHSQVVELVLNE
                     VAEAIKAGGEDLQIYTSGATNIFRYPELSEGDKASKLIGTLEHKEMLQEFVSEVNTEN
                     GGDAGIQVYIGDETPVQSMKDCSVVTANYDLGGGLRGTVGIIGPKRMDYEKVLGTLRN
                     LMSQLDAAFKKDER"
     gene            6878..7468
                     /gene="grpE"
                     /locus_tag="ACZPCG_09800"
     CDS             6878..7468
                     /gene="grpE"
                     /locus_tag="ACZPCG_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003498600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HIR1285385.1"
                     /translation="MSEDNKKEEVTDIPETEEAADAVKEETAEAETQTEEAADNASKG
                     GFFKKKKDPRDEKIEELTDRVKRQMAEFENFRKRTEKEKSSMYEMGARDIIERILPVI
                     DNFERGLAVIPEDKKEDPIAEGMEKIYKQIQKVFEEAGVKPIEAAGKEFDPNFHNAVM
                     HVEDESLGENIVAEELQKGYMYRDSVVRHSMVKVAN"
     gene            7507..>8642
                     /gene="dnaK"
                     /locus_tag="ACZPCG_09805"
     CDS             7507..>8642
                     /gene="dnaK"
                     /locus_tag="ACZPCG_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HIR1285386.1"
                     /translation="MGKIIGIDLGTTNSCVAVMEGGKPTVIANAEGVRTTPSIVAFTK
                     TGERLVGEPAKRQAVTNADRTISSIKRHMGTDYKVDIDGKKYTPQEISAMILQKLKAD
                     AESYLGETVTEAVITVPAYFNDAQRQATKDAGKIAGLEVKRIINEPTAAALAYGLDNE
                     NEQKIMVYDLGGGTFDVSIIEIGDGVIEVLSTNGDTHLGGDDFDNAVTNWMIAEFKKA
                     EGVDLSADKMALQRLKEAAEKAKKELSTATTTNINLPFITATAEGPKHLDMNLTRAKF
                     DELTHDLIERTAIPVQNALKDAGITASELGKVLLVGGSTRMISAQEKVKQITGKEPSK
                     SLNPDECVAIGAAIQGGKLAGDAGAGDILLLDVTPLSLSIETMGG"
BASE COUNT         2581 a         1646 c         2346 g         2069 t
ORIGIN      
        1 atatgctata cggcggtatc cctctggttg tccttcgtga gggagcaaac gataaggcag
       61 ctgcattaca gaatctgttc aatgagattt atcttcgaga tattaccaaa cgtaaccgag
      121 tgaagaacat tggagaactg gaagatctgc tgaatattct ttcttctgcc attggttcgc
      181 tgaccaatcc ggaaaaactg aagaatacgt tcaagacgat aaagaaatcc agaatcactt
      241 ccgctaccat taagaaatat ctggattatt ttgaggattc ttttctgatt gaatcagcac
      301 aaagatatga tattaaagga aaggcatata tcgaaactcc aaagaaatcg gaacgaaccc
      361 gtggcagaac cagaacccgg agtgggagac tacggagcgg aacttcattt atgatgacaa
      421 cgggcagctt ataaagtgca cggagagcag ggggcagttt gacaaggcca cctatactta
      481 tgagtatgac gaggccggga accgcacctt cgcaaagaaa gagaagcttt acagctatct
      541 ggagtcctgg cagatccgaa atcaatggcg taaaatattc ggatattgat tatattaacc
      601 gtatcttaaa agtggaatga cagttaggaa gagatgttta tgctgacaac agagagatca
      661 tagcggatgg agtaccggaa atagaggagt atatgaacat ggtgtttcca gatatggaag
      721 aaaatgagct ctttaaaaac gaactgctgg agattgtggt ggatgtaaca ggattccttc
      781 cggagactgc gtaaaagcaa aagaacagat gttcactgct tctgtacttt tgaaagaact
      841 tgtgatatag tagacgcaaa gagtctactg tcaatggcag tcgccaaatg cctgcaagca
      901 gtcacttgac tcgttgcttt gtggtatacg aaagtgcaat gtctgagtcc tttttatatg
      961 gtttgttcct gtttcgtcca tatagaatta catgggtacg gcaggttgca aaaaaagcag
     1021 attgtgaaca aaattcgtcg tgggcctatt ttgccgcaat ctgagaaagc gccacgacga
     1081 aaatgaaaag ttccttgacg aatttttgcc ttataggcgt ccttttttac tttgaaatct
     1141 ggaggtggat tttatgcaaa atgaacaaaa aatcttcaaa ttacattccg aatacgcccc
     1201 caccggcgac cagccccagg ccattgaggc gttggtcaaa gggttccagg aaggcaatca
     1261 attccagact ttactagggg ttacgggttc cggaaagacc tttaccatgg ctaatgtgat
     1321 tgcccagtta aataagccga ctttggtaat agcgcacaat aagaccttag cagcacagct
     1381 ctatggtgag ttcaaagagt attttccggg taacgcagtg gaatattttg tctcctatta
     1441 tgactactac cagcccgagg cctacgttcc ctccacggac acctacattg aaaaggactc
     1501 cgccatcaac gatgagatcg acaagctgcg ccattcggct acagcagctc tttccgagcg
     1561 cagcgatgtg atcatcgtgg catctgtatc ctgtatttac ggtctgggaa gtcccattga
     1621 ttatcaggag atggttattt cactgagacc ggggatggaa aaggaccggg atgatgtgat
     1681 ccgcaagctg atcgaaatcc agtatgaccg caatgacatg gactttaagc ggggaagctt
     1741 tcgggtcaag ggtgatgttc tggacattta tccggcctac tcagacggtc aggctttccg
     1801 ggtggaattt tttggggatg aggtagaccg gatcatggaa attgataccg tgactggaga
     1861 ggtgaaggcc cagctggggc atgtggcgat ttttccggca tctcattatg tggttcccaa
     1921 ggaaaagatg atggaggcca cggaacatat tttagaggaa ttaaaggagc gtgtatcatt
     1981 ttttaagagt gaggataagc tgttagaggc tcagaggatt tccgaacgca cgaattttga
     2041 tgtggaaatg atgcgggaga caggcttttg ttcgggtatt gaaaattatt ccagacattt
     2101 gacgggaaca gcaccgggag agcctccctg taccctgatt gattatttcc ccaaagactt
     2161 tctcattatt gtagatgagt cccacatcac tctgcctcag gtacggggca tgtatgcggg
     2221 agaccgttcc agaaaaatga cgctggtgga ctatgggttc cgccttcctt ctgccctgga
     2281 caaccgtcct ttaaattttt cggaatttga aagcaagatt gatcagatgc tgtttgtatc
     2341 agctacgccc tctgtctatg aacaggagca tgagctgatg cgggtggagc agatcatccg
     2401 tccgaccggg cttctggatc cggaaatatt tgtccgtcct gtggaggggc agattgacga
     2461 tctgatttca gaggtgaaca aggaggtggc aggccatcat aaggttctaa tcactacttt
     2521 gacaaagcgg atggcagagg atcttaccga ttatatgcgg gaagtgggaa ttcgggtgaa
     2581 atatctccat tccgatattg ataccttaga gcgtgcgcag atcatccggg atatgcgcct
     2641 ggatgtgttt gacgtgcttg tagggattaa tcttttaaga gagggccttg atataccgga
     2701 gattacgctg gtggcgattc tggatgcgga caaggaaggt tttctccgtt cagaaacctc
     2761 acttattcag acaattggcc gtgcggcacg gaattctgag ggtcatgtga tcatgtatgc
     2821 cgatagcatc acagattcca tgcgggtggc gattgaggag acgaaacgcc gccgtcagat
     2881 ccagcagaga tataatgagg agcatggcat tacgcctaca accattaaaa aggcagtaag
     2941 agacctgatc accatttcaa aggccgtgga aaatgacagt ttcgcagata cgaaggatcc
     3001 ggaatctatg gatgagaagg aacttaaaaa gctttcaaag gaactggaaa agaagatgcg
     3061 tcaggcagca gccgagttaa actttgagga ggcggctcgg ctcagagacc gtatggtgga
     3121 agtgaaaaag ctgcttttgg atatggaata gtctgggaac cggttggggc tgtaaggaat
     3181 tgtaaactct ttgtaagaat tatgaaattg cataaatcca aactttcctt tataataaaa
     3241 tcatacaaaa gaaacaaaac gaatcttaaa tataaggagg agaggtatat gtatacaaag
     3301 aaaatgtttt ccctggcaat ggcagcgttt ttgtcagcta tggctgtatt tccggtctat
     3361 gcgggcacgg agaagatcga tacagtgcgt ttggaatttt cttatgataa ggagccggag
     3421 agcggagatg atattggtga tatcagcgta agggccagag acgattcata tgatgtggat
     3481 tccgtagagt acacgaatct ggaggataag gatgtctgga cggtaggaga tgttcccgag
     3541 gtaaaggtag aactttctgc ttctgaggga taccgttttt cctataccag caagagccac
     3601 tttaagataa gcggctgtga cgctgagttt aaaaaggcaa aaatttatga tgacggcgat
     3661 tatatggaag tgacggtaga gctggaccgt atcggaggca agctggaggc tgctgataat
     3721 ctggactgga atgacagcac ggctgagtgg gacgaggtag agggttccaa gagctatgat
     3781 gtaaagctgt taagagatga caaaacggtt acaactgtca atacatctaa tacgtatttt
     3841 gacttttccg gatattttaa taaagaagga gattatacct tccgtgtgag agctattgca
     3901 agctataatg acaaggcagg cgagtggtct gaggattctt cttccctgta tgtagatgag
     3961 gatgaggcag gttcttacgg tggaagcgga cgctgggtac aggaaggcgg acgctggtgg
     4021 tattcttact ctacaggcgg ttatccggca gatggctgga aacagatcga tggagtgtgg
     4081 tatctctttg accgttccgg atatatgctt actggctggc agaaggtaga ccgtcagtgg
     4141 tattatcttg gttccaatgg tgctcttaca accggctggc agaatgtaaa cggacagtgg
     4201 tattatctca atggaagcgg tgagatgcag acgggctggc agtatgtaag cggcagatgg
     4261 tattatttaa acagcagcgg cgtaatgcag acaggatggc tggagcagaa cggacagtgg
     4321 tattatctgg atggaagcgg cgctatgaca accggatggc agtctgtgaa tggcagatgg
     4381 tactatatgg gcggaagcgg agtgatgttt agaaatacct ggacaccgga tggaagatat
     4441 gtagacggat cgggagcctg ggttcagtaa ggattgtata aaggataggt aaaaagagga
     4501 gctgctgtaa cggatgaaag ggttcgtaca gcagctcctt ttgtatgctt taattgaggg
     4561 gaagcatgca ggaagatacg atctcaatcg aatcagatct ccggctgtag cgatagatct
     4621 ggttggagag gcggtcctga ggcagaacct cggaggcatt gatctgggct accatttggc
     4681 tgagttcgtc ataagaaatg tcttcttcat cgggaagaag cagaacttca tggatactgg
     4741 agggaagaat cagcacgtcg cgttcaatcc cttctgagaa atgtttaaga acatcaggat
     4801 agagcataca gattgctccg ttgattccgc aggtattgga aagaacatag aaaggggtca
     4861 tggcctgtct gttcaggtcg gggacggagt ctgtcagatc ctctaaaata cagtccatac
     4921 tggtaatgga cggaggaaag agctgggaaa cattggagat ggcagcctga aagagttcat
     4981 cctgggaaat accccaggta ttcagatgag aggtatggat tccggcagtc attataccgg
     5041 tatcattttc ctgaagaaac aggtagaata cgatggaaag atcgttccat ggaatatgag
     5101 gcatatcctg caaaagtgtt tcattggcag ccgtgtttat aaggcggcag gcggtccggg
     5161 atttcatggc gctgtaatcg gaaagccggg ccggatgaat ggtgggcaaa gcctcattgg
     5221 agcggtacag ttccaaaagc tcctgagtga tttcctccaa agagcagcct tccagatact
     5281 gatggtagca gttattgaga taaatagcag gagctaccgg atttttgtct ttggaaatgc
     5341 acagaccatc cagaatcagg ccgttgtttt tggtgatcct gtgcaggctc agttcatact
     5401 cctttcccag ggcctgttcc atgcccgttt tgattttgtt gagaaatacg tcgtatacca
     5461 aatgtatacc cccttagtac attatattta tgtaaactgc aaaaggcagc ttcatagcag
     5521 gtaagaaaaa tattgtccgg atatcattcc tgaacaggat ctgaaacgta tataaaagaa
     5581 tgtttagaag gttacaaaac agacagaaat tctgatttgt gaaataaaaa tgaatctatg
     5641 tccagaataa aggcagagag gacttttgtc aagagaaaac tgtatataca ggaaataaga
     5701 tcatgaaaaa aatgaaaaat cccttgacaa aacagagaga aaggaatata ttatgaaatg
     5761 tagatgttag cactccaaag cattgagtgc taataatcgc aggagaggaa ggtgacagga
     5821 aacgatggat acacagctgg ataaccggaa gatcaccata ttaaaggcga tcatcaaaac
     5881 ctacctggag acaggcgaac cggttggttc cagaaccatt tccaagtatt cagaattaaa
     5941 actgagttct gccaccatcc gtaacgagat gtcagatctg gaagagatgg gatatatcat
     6001 tcagccccac acttccgcag ggcgtattcc ttccgataag ggatacaggt tctatgtaga
     6061 ccagatcatg caggaaaagg acaatgaagt tacagaattt aaggatatga tggttcagaa
     6121 ggttgataag ctggaactgg tccttaaaaa gatggctcag gttctggcgg ccaataccaa
     6181 ttatgcggcc atgatcagcg gccccagcta ccacaagaca aagctgaaat ttatccagct
     6241 gtccagagta gaggcacata agcttctggt agtggttgtg gtagaaggaa atatcgttaa
     6301 aaataccatg atcgatattg cggaagagct gggggaacag gaactcttga atctgaacat
     6361 cctgttaaac agcagcttaa acgggctgac gattgaagaa atcaatctgg atatcatcag
     6421 tcgtctgaag gaggatgcag gcattcacag ccaggtggtg gagctggtct taaacgaggt
     6481 tgcggaagcg atcaaggccg gcggagaaga tcttcagatt tataccagcg gggctacgaa
     6541 tatttttcgg tatccggagc tttcagaagg cgataaggcc agcaagctga tcgggacact
     6601 ggagcacaag gaaatgcttc aggagtttgt ttccgaggtc aatacggaaa acggcggtga
     6661 cgccgggatc caggtctata tcggtgatga aacgccggtt cagtccatga aggactgcag
     6721 cgtggttaca gccaattacg atctgggcgg aggacttcgg ggaacagtcg gaattatcgg
     6781 accgaagcgt atggattatg aaaaggtcct tggaacgctg cggaatctga tgagccagct
     6841 ggatgcagca tttaaaaagg atgaaaggtg ataagcagtg agtgaagata ataagaagga
     6901 agaagtaaca gatattccgg agacagagga agcggcagac gctgtcaaag aggaaactgc
     6961 ggaagcagag actcagacag aagaagctgc ggataacgcg tcaaagggag gtttctttaa
     7021 gaaaaagaag gatccgagag atgaaaagat cgaggagctg accgaccggg taaaacgcca
     7081 gatggctgaa tttgaaaatt tcagaaagag aacggaaaag gagaaatctt ccatgtatga
     7141 gatgggcgcc agagatatca ttgagcgcat ccttcccgtg atcgacaatt ttgagagagg
     7201 cctggcagta atccctgagg ataaaaagga agatcccatt gcagagggca tggaaaaaat
     7261 ctacaagcag atccagaagg tctttgagga agccggagta aagcccattg aggcagccgg
     7321 aaaggaattt gatcccaatt tccacaatgc agtgatgcat gtggaggatg agagcctggg
     7381 agaaaatatc gttgcggagg agctccagaa gggatatatg taccgggaca gcgttgtaag
     7441 acacagtatg gttaaagttg caaactaaaa tattttatag attgatcaaa tcaggaggaa
     7501 ataactatgg gcaagattat tggtatcgac ttaggtacaa cgaatagctg cgttgccgtt
     7561 atggaaggtg gaaagccaac cgttatcgca aatgcagagg gcgtaagaac cacaccatct
     7621 atcgtggcat ttacaaaaac aggtgaaaga ctggtaggcg agccagcaaa gcgtcaggca
     7681 gttacaaacg cagaccgcac catttcttcc atcaagcgcc atatgggcac agactacaag
     7741 gtagacattg acggaaagaa gtacacacca caggagattt ccgccatgat ccttcagaag
     7801 ctgaaggcag atgcagagag ctacttaggc gagacagtaa cagaggctgt tatcactgtt
     7861 cccgcatact ttaatgacgc acagcgtcag gcaaccaagg acgcaggaaa gattgcaggt
     7921 ctggaagtaa agcgtatcat caacgagcct acagcggcag cgctggctta cggccttgac
     7981 aatgaaaatg agcagaagat catggtatac gacttaggcg gcggtacctt tgatgtttct
     8041 attatcgaga tcggtgacgg cgtaatcgag gttctttcca ccaatggcga tacccatctt
     8101 ggcggcgatg actttgacaa tgcagttaca aactggatga tcgctgagtt taagaaggca
     8161 gagggtgtag atctttccgc tgataagatg gctcttcaga gattaaagga agcagctgag
     8221 aaggcaaaga aggagctgtc tactgctact actaccaata tcaaccttcc gttcatcact
     8281 gcaactgctg agggaccaaa gcatctggat atgaacttaa ccagagcaaa atttgacgag
     8341 ctgactcatg acctgatcga gcgcaccgca attccggtac aaaatgcatt aaaggatgca
     8401 ggcatcactg cttctgagct tggaaaggtg ctgttagtag gcggttctac ccgtatgatc
     8461 tctgcacagg aaaaggtaaa gcagatcaca ggcaaagagc ccagcaagtc tttaaatccg
     8521 gatgagtgcg tagccatcgg tgctgcaatc cagggcggaa aactggcagg cgatgcaggc
     8581 gccggagata tccttcttct ggatgttacc ccactgtccc tgtccattga gaccatgggc
     8641 gg
//
LOCUS       DBMDUH010000116         8599 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_3736_length_8599_cov_6.683337, whole genome shotgun sequence.
ACCESSION   DBMDUH010000116 DBMDUH010000000
VERSION     DBMDUH010000116.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 8599)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8599)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8599
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3736_length_8599_cov_6.683337"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="ACZPCG_09810"
     CDS             complement(<1..446)
                     /locus_tag="ACZPCG_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007714798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HIR1285387.1"
                     /translation="METISLFFLIEAIGTIAFASSGAMTAIRKRLDLLGVIILGETTA
                     VGGGMLRDILLGRVPPSLFINPIYVIMAFVTVLVLFLIIRFNQHILTGPYIVIYERIM
                     NIFDAVGLAAFTVTGIDTAMMAGYGDYRFLSIFLGVLTGVGGGVLR"
     gene            complement(467..1474)
                     /gene="asnA"
                     /locus_tag="ACZPCG_09815"
     CDS             complement(467..1474)
                     /gene="asnA"
                     /locus_tag="ACZPCG_09815"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HIR1285388.1"
                     /translation="MELILPKKYDPRLTIRETQDAIKYIRDTFQKEMGRELHLERISA
                     PLFVEQSSGLNDNLNGVERPVQFDMAGLPGETLEVVHSLAKWKRIALKEYGFQPGEGL
                     YTNMNAIRRDEELDNLHSCYVDQWDWEKVITKEERTMETLMATVREIFKIIKHMQHEV
                     WYKYPQAVKELPDDIFFITTGELAQMYPDKTPKERENLITKEHGCVFLMQIGDKLADG
                     KPHDGRAPDYDDWKLNGDILFWFDTLNCALEISSMGIRVDEASLKEQLKKAGCEDRMN
                     LPYHRMLLNGELPYTIGGGIGQSRLCMLLLDRAHIGEVQASIWPADMRAVCSQHKIYL
                     L"
     gene            complement(1547..1777)
                     /locus_tag="ACZPCG_09820"
     CDS             complement(1547..1777)
                     /locus_tag="ACZPCG_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3006 domain-containing protein"
                     /protein_id="HIR1285389.1"
                     /translation="MICIVDRIEETTAVCQREDRSVFTLPLSCLPQGVKEGDVLREEN
                     GVFKTDPDETSERRKRIRQKMKNLFSLAPFFL"
     gene            complement(1826..2575)
                     /locus_tag="ACZPCG_09825"
                     /pseudo
     CDS             complement(1826..2575)
                     /locus_tag="ACZPCG_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595251.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
     gene            2886..3131
                     /locus_tag="ACZPCG_09830"
     CDS             2886..3131
                     /locus_tag="ACZPCG_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285390.1"
                     /translation="MARGVRRTHTEKLQSELSEVRAAISQYEDSLDELRSREKEINSQ
                     IEIERFKEVSELLKQRGMTLCDLKELLSSRETQAAGI"
     gene            complement(3136..4884)
                     /locus_tag="ACZPCG_09835"
     CDS             complement(3136..4884)
                     /locus_tag="ACZPCG_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003505747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-ATPase domain-containing protein"
                     /protein_id="HIR1285391.1"
                     /translation="MKSSNELKRLLESIDRKSYPAYKSAQGIYDFGDYQLCIDHVQGD
                     PFASPSKLSIFITHQKAGYPKTLFDAPHKKRAFEDDLVRRFYKETARYNFKAKGSGKS
                     GLISISCPGPEILERTACECCSDGIWIRLEAGFPANGRTINSGELIRILFDFLPQCVK
                     QVLFYKNRPVHEPEAVAELAEDQYFIRQELKRLELVSFVADGAILPRESGISSRPMKN
                     STPFSSPESMRITLTLPHHGNCSGMAVRRGITLIVGGGYHGKSTLLKALEAGVYDHIA
                     GDGREFVITDDTALKLRAEDGRSIRNVDISMFINDLPNKKDTRCFSTEDASGSTSQAA
                     AVIEGIESGSRVFLIDEDTSATNFMVRDDLMQKVINRSQEPITPFTNRAEDLFHKAGI
                     STVLVAGSSGAYFYIADTIIQMDSYVPLDITKKVKEICAGDNRPSIEPAPSFTLPKAG
                     RKFQIKAEKDHRKQDMNVKEGRRGREQGGRDDRIKVKVYGKDSIEVGRRPSELRFVEQ
                     LIDSEQTQALAQILRFCMEKRLLERYTVAETVAYIQKETAKGGLTAVSGCSYAAMGLC
                     MPRPQEIFACINRFRG"
     gene            5143..6333
                     /locus_tag="ACZPCG_09840"
     CDS             5143..6333
                     /locus_tag="ACZPCG_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HIR1285392.1"
                     /translation="MERKVGTISRGIRCPIIREGDDLAAIVVDSVLDAASSEGFELRD
                     RDVISVTESVVARAQGNYASIDAIAEDVKAKLGGETIGVIFPILSRNRFSICLKGIAR
                     GAKKIILMLSYPSDEVGNALVSLDQVDEAGINPYSDVLDLKRYRELFGENKHPFTGVD
                     YVDYYCELIREQGAEAEVIFANNPREIVKHTKNVLTCDIHSRMRTKRILKEAGAERVC
                     GMDDILTAPVNGSGCNEKYGLLGSNKSTEDTIKLFPRECSDLVYEIQKQILDRTGKHV
                     EVMVYGDGAFKDPVGKIWELADPVVAVANTEGLEGTPNEIKLKYLADNDFKDLSGAAL
                     KDAISQRIREKKSDLVGDMASQGTTPRKLTDLIGSLCDLTSGSGDKGTPIILVQGYFD
                     NYTN"
     gene            6469..7173
                     /locus_tag="ACZPCG_09845"
     CDS             6469..7173
                     /locus_tag="ACZPCG_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008399582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285393.1"
                     /translation="MTHTDQTLSARLAASERILIGIGPEWGLKSEKKKIRDCRLSDPE
                     QAEIKAAYEALYEMVKDKDYYLVTTLTDGAVYDTPFDRERITAPCGNIHWRQCSRACT
                     KDIWEEGELPDEFCPHCGAPLAGNTIKEENYIEEGYLPGWKRYLSWQAGTVNRTLTIL
                     ELGEGFASPTVMRWPFEKIIFFNRKALLYRVNEEFYQIPREVQERSIPVHGNSLEWIL
                     NEKTKEESECDGGNIQ"
     gene            7154..7828
                     /locus_tag="ACZPCG_09850"
     CDS             7154..7828
                     /locus_tag="ACZPCG_09850"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007714990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR1285394.1"
                     /translation="MAAISNDWLGPLSGEFKKPYYKKLYETVKHEYETRKIFPDPDDI
                     FNAFAFTPLEKVKVVILGQDPYHNDGQAHGLCFSVKTDVDVPPSLVNIYQELHDDLGC
                     YIPNNGYLKKWADQGVMLLNTVLTVRAHQANSHHDIGWEQFTDAAIRVLNEQDRPMVF
                     ILWGRPAQAKAAMLDNKKHLILKAPHPSPLSAYRGFFGSRPFSQTNYFLTQNGLDPID
                     WQIENI"
     gene            7920..>8599
                     /locus_tag="ACZPCG_09855"
     CDS             7920..>8599
                     /locus_tag="ACZPCG_09855"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HIR1285395.1"
                     /translation="MSLVETLKIIVLGIVEGFTEWLPISSTGHMILVDEIIRLDQPEA
                     FKEMFLVVIQLGAILAVAVMYFHRLNPFSPSKNRRQREATWALWIKIIIACIPAAVLG
                     LLLDDWMEAHLYNAYVVAAMLILYGILFIVLENSPITSSPSIQKVGQISVKTGFLIGV
                     FQLLSLIPGTSRSGSTILGAMILGCSRSAAAEFSFFLGIPVMFGASFLKIVKFFLDGN
                     SFTGSQIF"
BASE COUNT         2194 a         2023 c         2170 g         2212 t
ORIGIN      
        1 tctctgagaa cgccgccgcc tactccggta agaactccca gaaagataga caggaaacga
       61 taatcgccat agcctgccat catggcagtg tcgatgcctg tgaccgtaaa ggcagccagc
      121 ccgacggcgt caaaaatatt catgatccgt tcgtagatta caatgtaagg gccggtaaga
      181 atatgctggt taaaacggat gataagaaaa agaaccagca cggttacaaa ggccatgatc
      241 acatagatcg ggttgatgaa caggctggga ggcacccttc caagaagaat gtctctgagc
      301 ataccgccgc cgacggcggt tgtttccccc aggatgatca ctcccagaag atccagacgc
      361 tttcggatcg cagtcatagc accggaggag gcaaaggcaa tagtgccgat agcctcaatg
      421 agaaagaaaa gagaaatagt ttccatggcg gcgccctcgt ttctctttac agaagataga
      481 tcttatgctg gctgcataca gcccgcatat cagcaggcca gatactggcc tgtacttctc
      541 cgatgtgggc acggtcaagc aaaagcatac acagacggga ctgtccgata ccgcctccga
      601 tggtgtaggg aagttctccg tttaacagca tcctgtggta aggaaggttc atgcggtctt
      661 cgcagccggc tttttttaac tgctctttaa gagaagcttc atccacgcgg atgcccatgc
      721 tggagatttc cagggcacag tttaaggtat caaaccagaa caggatatcg ccgtttaatt
      781 tccagtcatc atagtccggt gcgcgtccgt catggggctt tccatctgcc agcttatcgc
      841 cgatctgcat taagaatacg cagccatgct ctttggtgat caggttttct ctctcctttg
      901 gcgttttgtc gggatacatc tgtgccagct cgcctgtagt aataaaaaag atgtcatccg
      961 gaagctcctt tacggcctgg ggatacttgt accagacctc atgctgcata tgcttgatga
     1021 tcttgaagat ttcccgtacc gtagccatca gggtttccat ggtgcgctct tctttggtga
     1081 tcaccttttc ccagtcccac tggtctacat agcaggagtg gagattatcc agctcttcat
     1141 ccctgcggat ggcgttcata ttggtataga gaccttctcc cggctgaaag ccgtattcct
     1201 ttaaggcaat gcgcttccat ttggccagtg aatggacaac ctccagagtt tcaccgggaa
     1261 ggccggccat atcaaactgg accggacgct ctacgccgtt taagttgtcg tttaaaccgc
     1321 tgctttgttc tacaaacaag ggagcggaga tacgctccag atgaagctca cgccccattt
     1381 ccttctgaaa ggtatcgcgt atgtacttga tggcatcctg tgtttcacgg atcgtaagcc
     1441 tggggtcata tttcttgggt aatatcagtt ccattgatcc atcctccata aaaaagctct
     1501 gtatgctgtg ggatacaggc gtgataaaat tactcctatg gtatcattaa aggaaaaaag
     1561 gtgcaagaga aaaaagattt ttcatttttt gccggatgcg ttttcttcgt tcactggttt
     1621 cgtccgggtc agttttgaaa actccgtttt cttcccggag gacatctccc tcctttacgc
     1681 cctgaggcag acaggacagc gggagagtaa agacagagcg gtcctctctc tggcatacag
     1741 ctgtggtttc ttcgattcgg tcaacaatgc agatcataaa atatccctcc tatagcgttt
     1801 ctataatgtt ttattttttg gtataggtcc aggtaagtcc ttccggagaa ctggatgcga
     1861 tgatggtacc cttttcatcc gtacgcagta cctgaatgcg gtgttcagcg tattttttta
     1921 aggtttcctc atggggatgg ccgtaggaat tgcccttgcc gcaggaaatc acggcccagg
     1981 atggagatac agcttctaaa aattcagggc tgctggaggt gcggctgcca tgatggcctg
     2041 attttaatac atcagccgaa agattcaggc cgctgcgtgc catatcttcc tcagcctctg
     2101 tttccgcatc gccgcacatg acaaaggaat gatttccatc tgtgaggcgg atgcccacag
     2161 accagtcatt cagctcatct gcgtaatcgc ccacaggacc taagacggta aactgggact
     2221 gtcccagatc ataggtatca ccgggaacgg gctttgtgat cttaagcccc tggccggcca
     2281 ctgcatccag aacatcttca aaggttgcag ttgtatgttc aaccggcggc ataataacgg
     2341 ttttggccgg aaatgtttcc agtacgtcgt ccagcccgcc gatatgatcc gagtggggat
     2401 gggtaccgat cacatagtcc agagaggtga cgccctgctc tttcaggtag tttacaaccg
     2461 tatcgccctg atcattttcc cctgcatcga ccagcataaa atgagttccg tcctggatca
     2521 ggatgctgtc tccctggccc acatcaataa aatgtacatt taaggtgccg ctgctgcttt
     2581 gtatttcagg gttcgtttta tcatttaact gcccttttac cgcttccttt gcataatccc
     2641 aggcagccga aactgcctga tcggctgcgg attcaaggta tttggaggct tcctcctttg
     2701 agcagccacc aagagagaga agagaagcgg tcagtaaaat agtccctatg aaacgatatg
     2761 tctgttttgt catgtcttat cagcctttct gttatatttt cccattctgt cccatatata
     2821 actattatga gggacgattc cccgaagaaa acgaaacata ctaaattata aaggagttaa
     2881 tacctatggc aagaggagta agaagaaccc acacagaaaa acttcagtct gagctttctg
     2941 aggtccgggc tgcgatttcc cagtatgagg acagcctgga tgagctgcgt tcccgggaaa
     3001 aggaaattaa ttcccagatc gagatcgagc gttttaagga agtcagcgag cttttaaagc
     3061 agcgcggaat gacgctctgc gatttaaagg agcttctgtc ttcaagggaa acacaggcag
     3121 ccggaatctg actgctcagc ctctgaaccg gttaatgcag gcgaaaattt cctgaggtct
     3181 gggcatacac agtcccatgg cggcatagga gcagccgctc acagcagtga ggccgccctt
     3241 tgccgtctct ttttggatat aggcaactgt ttcggcaacc gtataccgtt ccagaagccg
     3301 cttttccata cagaaccgca ggatctgcgc cagcgcctgg gtctgctcac tgtcaataag
     3361 ctgttctaca aacctcagtt ctgacgggcg gcgtcctact tctatggaat cttttccata
     3421 tacctttacc ttgatccggt catcccgtcc gccctgttct ctgccgcggc ggccctcttt
     3481 tacgttcata tcctgtttcc gatgatcttt ttccgctttt atctggaatt ttcttcctgc
     3541 tttcggaagt gtaaagctgg gagccggctc aatggatggc cggttgtctc cggcgcagat
     3601 ttcctttacc ttttttgtaa tatccagggg gacataggag tccatctgga taatggtatc
     3661 tgcaatataa aaataggctc ctgagcttcc ggctactaaa acggtagaaa tgcctgcctt
     3721 atgaaacaga tcttccgccc ggttggtaaa tggagtgatc ggttcctggc tgcggtttat
     3781 gaccttctgc atcagatcgt cccgtaccat gaaattggtg gcggaagtgt cctcatcaat
     3841 gagaaacacc ctgcttccgc tttcgattcc ctcgatgaca gcggctgcct gggaggtact
     3901 gccgctggca tcctctgtgg aaaagcatct ggtatccttt ttgtttggaa gatcattgat
     3961 gaacatggaa atgtcaacat tccggatgct tcttccgtcc tcagcccgaa gctttaaggc
     4021 tgtgtcatcg gtgatcacaa attcacgccc gtctccggcg atatggtcgt atactccggc
     4081 ctccagcgcc tttaacaggg tggattttcc gtgataccct ccgcccacga tcagggtgat
     4141 tccgcggcgg actgccatgc cggagcagtt tccgtgatga ggaagagtaa gtgtgatccg
     4201 catggattca ggggaagaaa agggagtgct gtttttcatg ggccggctgg atatgccgct
     4261 ttcccttgga agaatggcgc cgtctgccac aaaggagaca agctccagac gttttaattc
     4321 ctgacggata aaatactgat cctctgcaag ctctgccacg gcctctggtt catggaccgg
     4381 tctgttttta taaaaaagca cctgttttac acactggggc agaaaatcaa agaggatccg
     4441 tatcagttca ccgctgttga tggtgcggcc gtttgcggga aaaccggctt ccagccggat
     4501 ccagattcca tctgagcagc attcacaggc agtccgttcc aaaatttccg gtcccggaca
     4561 gctgatggaa ataaggccgc tttttccaga tccctttgct ttaaaattgt accgggcggt
     4621 ctccttataa aaccggcgca caagatcgtc ctcaaaggct ctttttttat ggggagcatc
     4681 aaaaagtgtt tttggatatc cggctttttg gtgggtaata aaaatactca gcttggaggg
     4741 agaagcaaag gggtctccct gcacatggtc aatacataac tggtaatcgc caaagtcata
     4801 gatgccctgg gcggatttat aggccggata gcttttccgg tcaatggatt ctaacagacg
     4861 tttaagctcg tttgatgatt tcatgcttct atacaccttt cgcatccgga atttttcccg
     4921 gactttcagg tatcatttta agcgaacaca cagaaaaatg caacgcctgc aaacgttcct
     4981 aaggttttct gatgaccttc aaaggcatcc tgcacaccag atttcaggga gccctgcaaa
     5041 aaaatattcc caaaaccaat aatttgagtt ataataaaat agagtcgttt tgtgtggctt
     5101 tttactccac aaaagaaatt tatagactag gagacaatac atatggaaag aaaagtagga
     5161 acaatctcaa gagggatccg ctgcccgatc atccgtgaag gcgacgatct tgctgcgatc
     5221 gtggtagaca gcgtactgga cgcagcttcc agcgagggct ttgaactgcg tgacagggac
     5281 gtaatctctg ttactgagtc tgtagtagcc agagctcagg gaaactacgc ttccatcgat
     5341 gccattgccg aggatgtaaa agcaaagctg ggcggagaga ccatcggcgt tattttccca
     5401 attttatcca gaaaccgttt ttccatctgc ttaaagggaa ttgccagagg cgcaaagaag
     5461 attattctga tgctcagtta tccaagcgat gaggtaggaa acgccctggt gtctctggat
     5521 caggtagatg aggctggaat caatccttac agcgatgttc tggatctgaa gcgttacagg
     5581 gagctttttg gagaaaataa gcatccattt acaggcgtag actatgtaga ttactactgt
     5641 gagctgataa gagagcaggg cgcagaggca gaagttatct ttgcaaataa tccaagagaa
     5701 atcgtaaagc acaccaagaa cgttcttacc tgcgatatcc attcccgtat gcgcaccaag
     5761 cgcatcttaa aagaagcagg agcagagaga gtctgcggta tggacgatat cctgacagct
     5821 cctgttaacg gcagcggctg caacgagaag tacggacttt taggctccaa caagtctacc
     5881 gaggatacca tcaagctgtt cccaagagag tgcagcgatc tggtatatga gatccagaag
     5941 cagatcctgg atcgtaccgg aaagcacgtt gaggttatgg tatacggtga cggtgcattt
     6001 aaagatccgg taggaaaaat ctgggagctg gctgacccgg ttgttgcggt tgcaaatacc
     6061 gagggtctgg aaggaactcc aaacgaaatc aagttaaaat atctggcgga caacgatttt
     6121 aaggatctgt ccggcgcagc attaaaggat gcgatctctc agaggatcag agagaagaag
     6181 tccgacctgg taggggatat ggcttctcag ggaacaactc cgagaaaact tacagatctg
     6241 atcggatctc tgtgcgacct gaccagcggt tccggcgata agggaactcc tattattctg
     6301 gttcagggat attttgataa ctataccaat taaagcaggc ttttttcaga gtgatgcagc
     6361 agcctgatgc ttatgtgggg caaaatccgg cagaggttct cgcaagggga tttctgccgg
     6421 atttttacca ggctgcagcg gcagaggaac gtacacaata tggaatatat aacacataca
     6481 gaccagaccc tgtcagccag actggctgct tcggagcgga tcctcatcgg gatcgggcca
     6541 gagtgggggc taaagtctga aaaaaagaaa atccgggatt gcaggctttc agacccggag
     6601 caggcagaga taaaggccgc ctatgaagcg ctgtacgaga tggtgaagga taaggattac
     6661 tatctggtca cgactcttac ggacggagcg gtttatgata cgccctttga cagagagcgc
     6721 atcacggctc cctgcgggaa tattcactgg cgtcagtgca gcagggcctg tacaaaggat
     6781 atctgggagg agggagaact tccagatgag ttctgccccc actgcggcgc tcctcttgcg
     6841 ggaaatacca taaaggaaga aaactatatt gaggaaggct atcttcccgg gtggaagagg
     6901 tatctttcct ggcaggccgg gactgtgaac cgtacactaa ctatattaga gctgggagag
     6961 ggctttgctt ctcctacggt tatgcgctgg ccttttgaaa agatcatttt ctttaacaga
     7021 aaggctcttt tgtaccgggt caatgaagaa ttttatcaga tacccaggga ggtacaggaa
     7081 cgcagcattc cggtgcatgg aaattctctg gaatggattt tgaatgaaaa aacaaaggaa
     7141 gagagtgagt gtgatggcgg caatatccaa tgactggctg ggccctttaa gcggggaatt
     7201 taaaaagcct tattataaaa agctgtatga aacagtaaaa catgaatatg agaccagaaa
     7261 gatatttccc gatccggacg atatcttcaa cgccttcgcc tttacacctc tggaaaaggt
     7321 aaaggtagtg atcctgggac aggatcccta tcacaatgac ggtcaggccc atggcctttg
     7381 cttctccgta aaaacggatg tagatgtgcc gccgtctctt gtaaatattt accaggagct
     7441 tcacgacgat ctgggctgct atattcccaa taacggctat ctgaaaaaat gggcggatca
     7501 gggagtgatg cttttgaata cggtgcttac ggtccgggcc catcaggcta actcccacca
     7561 tgatataggc tgggaacagt ttacggatgc agccatacgg gtactcaacg agcaggaccg
     7621 cccgatggta tttatcctgt ggggaaggcc ggcccaggcc aaagccgcta tgctggataa
     7681 taaaaaacac cttattttaa aggcgcctca tccaagcccg ctgtctgcct atcggggatt
     7741 tttcggaagc cgtcccttca gccagaccaa ttattttctc acgcagaacg gtctggatcc
     7801 cattgactgg cagattgaaa atatctgacc gtctgcagaa ggcatgggag agtgaggaaa
     7861 cgcatacgat ataccgtcgg aagacggttc taaaacatac ggtgtaaaaa ggagaaacta
     7921 tgagtttggt agaaacatta aaaatcatcg tactgggaat tgtggaggga tttacagagt
     7981 ggctgcctat cagcagcacc ggccatatga tcctggtgga tgagatcatc cgtctggacc
     8041 agccggaggc atttaaggaa atgtttctgg tagtgatcca gctgggggca atcctggcag
     8101 tggcagtgat gtattttcac aggctcaatc cattcagccc ttcaaaaaac cgcaggcaga
     8161 gggaagcaac ctgggctctg tggatcaaga tcatcattgc ctgcattccg gcggctgttc
     8221 tggggctttt gttggatgac tggatggagg cccatctgta taatgcctat gtggtagcgg
     8281 caatgctgat cctctatggt attttgttca ttgtactgga aaacagtccc atcacttcca
     8341 gtccatcaat ccagaaggtg ggacagattt ctgtgaaaac aggcttcctg atcggcgtat
     8401 ttcagctgct ttcgttgatt cccggtacct cccgctcagg ctctacgatt ttaggcgcta
     8461 tgatcctggg ctgttcaaga agcgctgccg ctgagttttc ctttttcctg ggaatcccgg
     8521 tgatgtttgg agccagcttt ttgaaaattg taaaattttt cctggatgga aattccttta
     8581 caggatctca gattttcta
//
LOCUS       DBMDUH010000117         8375 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_3824_length_8375_cov_6.174039, whole genome shotgun sequence.
ACCESSION   DBMDUH010000117 DBMDUH010000000
VERSION     DBMDUH010000117.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 8375)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8375)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8375
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3824_length_8375_cov_6.174039"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /gene="coaBC"
                     /locus_tag="ACZPCG_09860"
     CDS             <1..763
                     /gene="coaBC"
                     /locus_tag="ACZPCG_09860"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HIR1285396.1"
                     /translation="VIRPATGYLACGDTGAGKMPEPELLLEYIRCALAPKDLKGKKIL
                     VTAGPTREAIDPVRYITNHSTGKMGYAIAEAAAYRGADVTLVTGPVSIKAPLFVKTVP
                     AESAKQMYEAVTGAAKEQDIIIKAAAVADYRPAHQAEDKIKKSEGDMKIELERTDDIL
                     GWLGAHRHEGQFLCGFSMETRDLLANSRAKLERKNIDMIVANNLKTEGAGFGTDTNVV
                     TLITKEGQTELPKMTKQEVAHRLLDEIRNRLDFTL"
     gene            920..2524
                     /locus_tag="ACZPCG_09865"
     CDS             920..2524
                     /locus_tag="ACZPCG_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004853000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HIR1285397.1"
                     /translation="MSQTDDQMIEQIKKRRTFAIISHPDAGKTTLTEKFLLYGGAINL
                     AGTVKGRKAAKHAVSDWMDIEKERGISVTSSAMQFNYDGFCINILDTPGHQDFSEDTY
                     RTLMAADSAVMVIDGSKGVEAQTIKLFKVCVMRHIPIFTFINKFDREARDPYELLDEI
                     EEVLGIRTCPVNWPIGCGKNFKGVYDRFTKKISTFTAAMGGAKEVATREFEAESPEAE
                     SAVGSIYYQQLMDDIELLDGASDELDMDRVQRGDLSPVFFGSALTNFGVETFLEHFLK
                     MTTSPLPRKSLTGEIDPFRKDFSAFVFKIQANMNKAHRDRIAFMRICSGKFEAGMEVN
                     HVQGGKKIRLTQPQQLMAQDRKIVEEAYAGDIIGVFDPGIFSIGDTLCTSNEKFEFEG
                     IPTFAPEHFARVRQVDTMKRKQFIKGINQIAQEGAIQIFQEFNTGMEEIIVGVVGVLQ
                     FEVLTYRLQSEYNVEVILEKLPYEHIRWVENPQEVDVARLMGTSDMKRICDLKGNPLL
                     LFINSWSVGMVLDRNPGLKLSEFGTN"
     gene            2580..3026
                     /locus_tag="ACZPCG_09870"
     CDS             2580..3026
                     /locus_tag="ACZPCG_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HIR1285398.1"
                     /translation="MSKVEVVRAAMVQAMKAKDKARKESLSMLLSALKNAEIDKREPL
                     TEAEENAVVKKEIKQTQETYDLAPADREDIRSEAAQRLAVYKEFAPEDMSVDQIRQVI
                     AEVLAELGIDAPSPKDKGSIMKVLMPRVKGKADGKVVNETLAGMFK"
     gene            3067..4470
                     /locus_tag="ACZPCG_09875"
     CDS             3067..4470
                     /locus_tag="ACZPCG_09875"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006779802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HIR1285399.1"
                     /translation="MYRKEIDGFIDSHRQEMIDDICKLCRINSEKMPYTEGKPYGDGA
                     FDALHAALAMMEDYGFSITNYDNYVGTADLNDKERQLDILAHLDVVPAGEGWTVTEPF
                     EPVEKDGKLYGRGTADDKGPAVAALYAMRAIKELGIPVKKNVRLILGTDEECGSSDIE
                     VYYKREKEAPMTFSPDASFPVINIEKGRIPGGFSASFEASEALPKIVSIDAGIKVNVV
                     PGKAKAVVAGLEAEVLTQAADAVEKQTGLSFSFGETEDGIQITVEGKGAHASTPDEGN
                     NALTGLLSYLVSLPLADCPQLQAVKSLVTLFPHGDTRGKAVGIAMEDELSGPLTLAFD
                     MLTLDKDHLEGAFDCRACLCATEENSLNVIRARMAEEGLTLQADHMTAPHHVDGNSHF
                     VKTLLKAYEEYTGLEGHCESTGGGTYVHHLENGVAFGASMPGTDNKMHGADEFAVIDE
                     LLTSAKIFAQVIVDLCC"
     gene            4847..5863
                     /locus_tag="ACZPCG_09880"
     CDS             4847..5863
                     /locus_tag="ACZPCG_09880"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007157912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HIR1285400.1"
                     /translation="MDQNKEEAIIRLEGLGKQFATSNGTVTALDDINLEIRRGEIFGI
                     IGLSGAGKSTLVRCINYLEEPTSGRVIFEGKNLSAMKEKEKRLARQSMGMIFQQFNLL
                     AQRNVLQNVCFPMEIAHMDSRKAKERAKELLKLVGLEERMNAYPAQLSGGQKQRVAIA
                     RAMATNPRVLLCDEATSALDPNTTKSILELLKKINREMGITVIMITHEMAVIEAICDR
                     VAIIDHSHIAESGKVSDIFSGPKSDIGRQLILGDTSDQTKNFGGSRMIRVIFDGRESS
                     EPIIANMILACKAPVNIMHADTRDIEGKAMGQMLLQLPEDTVDADRICNYLRTAGVTF
                     EEVH"
     gene            5866..6531
                     /locus_tag="ACZPCG_09885"
     CDS             5866..6531
                     /locus_tag="ACZPCG_09885"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HIR1285401.1"
                     /translation="MTFDQTTIDMLLKGIWETIYMVSLSSFLSYVIGIPVGIALVVTD
                     RDGISPFPLFNKVLGVVINLLRSIPFIILLVMVIPFTRMIVGKIIGPNAAVVPLVISA
                     FPYISRMVESSLKEVDAGVIEAAKSMGASAGQIIWKVLLPEAKPSLLVGAAISITTII
                     GYSAMAGTVGAGGLGDIAIRYGYHRYETEMMLITVALLVVIVQIVQEVFMRISRKSDK
                     RIR"
     gene            6605..7492
                     /locus_tag="ACZPCG_09890"
     CDS             6605..7492
                     /locus_tag="ACZPCG_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR1285402.1"
                     /translation="MKRKTLETAVIAAALAASLLTGCSKPAEETTAAAEKQESKADAG
                     QEKKESPEKLEELTVGASPAPHAEILEAAREVLASKGYDLKIVEYTDYVLPNNALDSG
                     DLDANYFQHQPYLDSFNAQNGTELSGVAVIHYEPFGIYAGKSASLDAIPEKGTVLVPN
                     DLTNEARALLLLEAQGLITLKEDAGLEATKNDIVENPKNLDIVEIEAAQLPRSLQDGD
                     VAVINGNYAIEAGLKVSDALATEDAESLAAQTYGNVIAVRAGEEESPKTKALIEALTS
                     DEVKSFMESTYEGAVVPVF"
     gene            7609..7869
                     /locus_tag="ACZPCG_09895"
     CDS             7609..7869
                     /locus_tag="ACZPCG_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HIR1285403.1"
                     /translation="MTICDLKQKEVINIKDCKRLGFVGDVDFDMKTGCMTALIVPGPG
                     CICGFLGREKEYIIPFCDICQVGNDIILVDVKEKNVEEKVKC"
     gene            complement(8106..>8375)
                     /locus_tag="ACZPCG_09900"
     CDS             complement(8106..>8375)
                     /locus_tag="ACZPCG_09900"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HIR1285404.1"
                     /translation="LGADIAKIAVMPRCPEDVLTLLTATVTARRQLTCPVVTMAMAGT
                     GLISRVWGEIFGSCLTFGLAGQASAPGQIDARELKCVLDILHNNL"
BASE COUNT         2310 a         1819 c         2305 g         1941 t
ORIGIN      
        1 ggtcatccgt ccggcgacgg ggtatctggc ctgcggcgat acaggggcgg gaaagatgcc
       61 ggagccggag ctccttctgg aatacatccg atgcgccctt gctcccaaag acttaaaggg
      121 aaagaaaatt cttgttaccg caggccctac cagagaggcc attgatccgg tgcgctatat
      181 tacgaatcat tccacaggga aaatggggta tgccattgca gaggcggctg cataccgcgg
      241 agcagatgtg acccttgtca caggccccgt atccataaag gcgcctctgt ttgtgaaaac
      301 cgtaccggca gagagcgcaa aacagatgta tgaggcagtt acaggggcgg cgaaagagca
      361 ggatatcatt atcaaggcag cagcagtggc agattaccgt cccgcccatc aggcagagga
      421 taagattaaa aaatccgaag gcgacatgaa gattgaactg gaacgcacgg atgatatttt
      481 aggctggctg ggagctcacc gccatgaagg ccagttcctt tgcggctttt ccatggaaac
      541 aagagacctt ctggcaaatt caagggccaa actggaacgc aagaacattg atatgatcgt
      601 ggctaacaat ctgaaaacag agggagccgg cttcggtaca gacactaacg tagtgaccct
      661 gatcaccaaa gaaggacaga cagagcttcc caagatgacg aaacaggagg tagcccaccg
      721 cctgttggac gagatcagga accgtcttga ctttaccctt taatcataat aaaatatgta
      781 ggataccagg ggcaaaaggt tcgaaatccg acgcaagcct gcttgcagga ggattttgga
      841 tcttgggcgg tgccccaaca tcatatgaac agctcgtccc cttaaggggc gcaatcagca
      901 ggaacggagg ataagaccat tgtcacagac agatgatcag atgatagaac agataaaaaa
      961 gcgcagaacc tttgccatta tttcccaccc cgatgcaggt aaaaccacac tgacagagaa
     1021 attccttctt tatggaggcg ccatcaatct ggcaggtacg gtaaagggcc gcaaggcggc
     1081 aaagcatgca gtttccgact ggatggatat tgagaaggag agaggtattt ccgttacttc
     1141 ttccgccatg cagtttaatt atgacggatt ctgcatcaac atcctggata cgccgggcca
     1201 tcaggatttt tcagaggata cttaccgtac ccttatggcg gctgattccg cggtcatggt
     1261 aattgacggc tccaagggtg tggaggccca gactattaag ctctttaagg tctgtgtcat
     1321 gcgccacatc ccgattttta catttattaa caaatttgac cgtgaggcca gagatccata
     1381 tgagctttta gacgagatcg aggaggtgct gggcatccgc acctgtccgg tgaactggcc
     1441 catcggctgc ggaaagaatt tcaagggcgt ttacgaccgc tttacaaaga aaatctccac
     1501 ctttaccgct gccatgggag gcgcaaagga ggttgcaacc agggaatttg aggcagaaag
     1561 cccggaggca gagtctgctg tcggttccat ttattaccag cagctgatgg acgacattga
     1621 gcttttggac ggcgccagcg atgagctgga tatggaccgg gtgcagaggg gagatctttc
     1681 tcctgtattt ttcggttctg cccttacgaa tttcggtgtg gagacctttt tggagcattt
     1741 cctgaaaatg accacctctc ccctgccaag aaagtccctt acaggagaga tcgatccatt
     1801 ccgcaaggac ttttccgcct ttgtctttaa aatccaggca aatatgaaca aggcccacag
     1861 agacaggatc gcttttatgc gtatctgctc cgggaagttc gaggcaggca tggaagtgaa
     1921 ccacgttcag ggcggaaaaa agatccgctt aacacagcct cagcagctta tggcacagga
     1981 tcgtaagatc gtggaagagg cgtatgcggg cgatattatc ggcgtatttg atccgggcat
     2041 tttctctatc ggagatacgc tgtgcacatc caatgaaaag tttgaatttg aggggatccc
     2101 gacctttgcg ccggagcatt ttgcaagagt gcgccaggtg gataccatga agcgcaagca
     2161 gtttatcaag gggatcaacc agattgccca ggagggtgcg atccagattt tccaggaatt
     2221 taacacaggt atggaagaga tcatcgtagg cgtagtaggc gtacttcagt ttgaggtatt
     2281 aacctaccgt ctgcagagcg aatacaatgt agaggttatc ctggaaaaac tgccttatga
     2341 gcatatccgc tgggtggaga atcctcagga ggtggatgtg gcccgtctga tgggaacctc
     2401 cgacatgaag cgcatctgcg acttaaaggg aaatcctctg cttttattta tcaacagctg
     2461 gagcgtaggt atggtattag accgcaatcc gggtcttaaa ttaagcgagt tcggcaccaa
     2521 ttagcagaca ggtttcctgt ccggctgctg tgccctcata gataaaggag gttttagaga
     2581 tgtcaaaggt agaagtagta agagccgcta tggtacaggc catgaaggca aaggataagg
     2641 caagaaagga gtccctttcc atgctgcttt ccgctcttaa aaatgcggag atcgacaaga
     2701 gagagcccct gactgaggcc gaggaaaatg cagttgtcaa aaaggaaatc aagcagacac
     2761 aggagacgta tgacctagct cctgcagacc gtgaagatat ccgcagcgaa gctgcccaga
     2821 gactggctgt atataaggaa tttgctccgg aggatatgag tgtggatcag atccgccagg
     2881 tgatcgcaga ggttcttgca gagctgggga tcgatgcgcc ctctcccaag gataagggtt
     2941 cgatcatgaa ggttctgatg cccagagtga agggaaaggc agacggaaag gttgtaaacg
     3001 aaacattggc aggtatgttt aagtaaataa gtgcccatac aaaaagggcg gcaaaaggag
     3061 gacaatatgt acagaaaaga aatagacgga tttatcgatt cccacaggca ggagatgatt
     3121 gacgatatct gcaagctctg ccggattaac agtgagaaaa tgccctatac cgagggaaag
     3181 ccttatggcg acggcgcatt tgatgccctt cacgctgctc tggctatgat ggaggattat
     3241 ggtttttcca ttaccaatta tgataactat gtgggaacag ctgatttaaa tgataaggag
     3301 cgccagctgg atatcctggc acatctggat gtggttcccg caggagaggg ttggaccgtt
     3361 acagagccct ttgagcctgt ggaaaaggat ggaaagctgt atggccgcgg tacggcagat
     3421 gacaaggggc cggcagtggc tgctctttat gccatgcgtg ccataaagga gcttggcatc
     3481 cctgtaaaga aaaatgtgcg cctgattttg ggaaccgatg aggagtgcgg aagcagcgat
     3541 atcgaggtgt actataagcg tgagaaggaa gcacccatga cattttcacc ggatgcctcc
     3601 ttccctgtga tcaatattga aaagggacgc atcccagggg gcttttctgc ttcttttgaa
     3661 gcttctgagg ctctgccaaa gattgtttcc attgacgctg gcattaaggt aaatgtagtt
     3721 cccggcaagg caaaggccgt agtggcaggt cttgaagcag aggttctcac tcaggctgcg
     3781 gatgcggttg aaaagcagac gggactttcc ttcagctttg gagagacgga ggacggaatc
     3841 cagattactg ttgaaggaaa gggcgctcat gcatccactc cggatgaggg aaataatgct
     3901 cttacaggcc tgttatccta tctggtaagc ctgccgctgg cagactgccc tcagcttcag
     3961 gcagtaaaga gccttgtaac cctcttccct catggtgata ccagaggaaa ggctgtagga
     4021 atcgccatgg aggatgaact ttccggtcct ctgaccctgg catttgatat gctcaccctg
     4081 gataaagacc acctggaggg agcgtttgac tgtcgtgcct gtctgtgtgc tacggaggag
     4141 aacagcttaa atgtgatccg cgcccgtatg gcagaggaag gccttacctt acaggcagac
     4201 catatgacag cgcctcacca tgtagacgga aactcccatt ttgtaaagac acttttaaag
     4261 gcctatgagg agtataccgg attagaggga cattgtgagt ccacaggagg cggaacctat
     4321 gtgcatcatc tggaaaatgg agttgccttc ggcgcttcca tgccaggtac tgacaataag
     4381 atgcacggag ccgacgagtt tgcagtgatt gacgagcttc ttaccagtgc aaagattttt
     4441 gctcaggtaa tcgtagatct gtgctgttaa ttgcctaatt gcctttcaga gccggatggc
     4501 aggaaaaaat tcttgacaaa ccggtataag tgcggtatct ttaaaactga cattattcct
     4561 agcagtatta taggaaatga ttaccagtcc aggcaggagg attttcatga atcagttcaa
     4621 agccatgcag aatatggata tgatgaacag catgtacatg tgctgtgtat ccgcgtgctt
     4681 tgaaaactga atgggaaacg atctgtatgg atttggaatc atgatatcaa tatattttgg
     4741 agaacgtatc attcggggtt gcaggtccgt gcaggactgc ggccccgttt tgtttcttag
     4801 ccggaggatg ggaaacaggg gaaggattag agaaaggaat gaagaaatgg atcagaataa
     4861 agaagaggcc atcatacgcc tggagggtct gggcaaacag tttgccacgt caaacgggac
     4921 cgtgactgct ttagatgaca tcaatctgga aatccgccgg ggagagattt ttggtatcat
     4981 cggtctttcc ggagcaggaa agagtaccct ggtgcgctgt atcaactatc tggaggaacc
     5041 tacctccggc cgggtaatct ttgagggaaa aaatctttcc gccatgaagg aaaaggaaaa
     5101 gcgtctggca agacagtcca tgggaatgat ctttcagcag tttaacctgc tggcccagag
     5161 aaatgtgctg cagaacgtgt gcttccccat ggagattgct catatggaca gcagaaaggc
     5221 caaagagagg gcaaaggagc tgttaaagct ggttggactg gaagagcgga tgaacgcata
     5281 tccggcccag ctttccggcg gacagaagca gagagttgcc attgcaagag ccatggcgac
     5341 aaatcccaga gttttattat gcgatgaggc aacaagcgcc ctggatccca atacgacaaa
     5401 gtccattttg gagctgctaa aaaagattaa ccgggaaatg gggatcacag tgatcatgat
     5461 tactcatgag atggcagtca ttgaggccat ctgcgaccgg gtggcgatca tcgaccacag
     5521 ccatattgca gagtcgggca aggtatcgga tatcttttcc ggtcccaaat ctgatatcgg
     5581 acgccagctg attttaggag atacctctga tcagacaaag aatttcggcg gttcccgtat
     5641 gatccgtgtg atttttgacg gacgggaatc ctctgagccg atcatcgcaa atatgatcct
     5701 ggcctgcaag gccccggtga atatcatgca tgcggatacc agagatatcg agggaaaggc
     5761 tatggggcag atgcttctgc agcttcctga ggataccgtg gatgcagacc ggatctgcaa
     5821 ttatctgaga acagccggag taacatttga ggaggtgcac tagagatgac ctttgatcag
     5881 acaacgatcg atatgctttt aaaaggaatc tgggaaacca tatacatggt ttcactttcc
     5941 tcatttttat cctatgtgat cggtattccc gtgggaattg cccttgtagt cacagacagg
     6001 gatggaatca gtccctttcc gttgtttaac aaggtgcttg gcgttgtgat caatctcctt
     6061 cgctccattc ccttcatcat ccttcttgta atggtcatcc catttacccg catgatcgta
     6121 ggaaagatca tcggacccaa tgcagcggta gtgcctctgg tgatttccgc gtttccttac
     6181 atcagccgta tggtggagtc ctctttaaag gaggttgacg caggagtgat cgaggcagcc
     6241 aagtccatgg gtgcatctgc gggacagatt atatggaagg tgcttcttcc agaggccaag
     6301 ccatcccttt tagtgggggc tgccatttcc attacaacga ttatcggcta ctctgccatg
     6361 gcaggtacgg taggtgcagg cggtctggga gatattgcca tccgttacgg ttaccacaga
     6421 tatgagacag agatgatgct cattacagta gcccttcttg tagtgatcgt acagattgtt
     6481 caggaagtat ttatgcgcat atcaagaaag agcgataaga ggatccggta aaacggatct
     6541 taagcggctt aaaaaccgca gaatacatat cagggccctg ggcctgaaaa gaggaggaac
     6601 tattatgaaa agaaagacat tagaaacagc agtaatcgca gcagcattag ctgcatccct
     6661 gttaaccggc tgctccaaac cggccgagga gacaacagca gcggcagaaa agcaggaaag
     6721 caaagccgat gcaggacagg agaagaaaga gagccctgaa aaactggaag agctgaccgt
     6781 aggcgcaagc cctgcccccc atgcggagat tctggaggct gccagagagg ttttggcttc
     6841 taaaggctat gacttaaaga ttgtagagta cacagattat gtgcttccga acaatgcgct
     6901 ggattccggt gatctggatg caaactattt ccagcaccag ccttatctgg attcctttaa
     6961 tgcccagaac ggcacagagc tttccggtgt ggcggtgatc cactatgagc cctttggtat
     7021 ctatgcagga aagagcgctt ctttagacgc gattccggaa aagggaaccg ttcttgttcc
     7081 aaatgacctg acaaatgagg caagagccct tcttctttta gaggcccagg gcctgatcac
     7141 cttaaaggag gatgcaggtc tggaagcaac gaaaaatgat attgtagaaa atccaaagaa
     7201 ccttgatatt gtagagatcg aggcagccca gcttccccgt tccttacagg acggcgatgt
     7261 ggcagttatc aacggaaact atgccatcga ggcgggctta aaggtaagcg acgctttggc
     7321 aacagaggac gcggaatctc tggcagccca gacctacgga aacgtgatcg ccgtaagagc
     7381 gggtgaagag gaaagtccaa aaacaaaggc tctcattgag gcgcttacga gtgatgaggt
     7441 aaaatcattt atggaaagta cttatgaggg tgcggttgtc cctgtattct aaattttata
     7501 tttgaaattc cctgtgcata gggagcagaa tgggccggaa atgaatactt ccggctcttt
     7561 ctgcgtatat atggataaga aataaaactg ccaggcagga ggggaaaaat gacgatttgc
     7621 gatctcaaac agaaagaggt cataaatata aaggactgca aacgcctggg tttcgtagga
     7681 gatgtggatt ttgatatgaa aacgggatgc atgaccgctt tgatcgttcc ggggcccgga
     7741 tgcatctgcg gttttctggg gagggaaaag gagtatatca ttcccttttg cgatatctgc
     7801 caggtcggaa atgatattat cctggtggat gtgaaggaaa agaatgtaga ggaaaaggta
     7861 aagtgttaaa cgactgcctg ccagtgacgg gggatcactg gcaggcagtc gttttataag
     7921 aaaaagctgt atgaaaagct tgtgcgggct tttgactttg gaagggagtg ccggagctgt
     7981 tgggggggat gaaccggcat tctgtaagga aacactgact aaaaactaac acattttttg
     8041 ctgaaaggga aggggaatta ctgaaaatga aatatattta ccattataga aataccctat
     8101 gtctgctaca gattattatg aagaatatcc agcacacatt ttagctctct ggcatcgatc
     8161 tgtccgggag cggatgcctg tcctgccaga ccaaaagtaa gacaggagcc aaagatttcc
     8221 ccccagacac ggctgatgag ccctgttccc gccatggcca tggtgactac aggacaggtg
     8281 agctggcgtc gggctgtaac ggtagctgta agcagcgtaa gcacatcttc cggacaccgg
     8341 ggcatcacgg cgatttttgc aatatcggct cccag
//
LOCUS       DBMDUH010000118         8367 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_3826_length_8367_cov_4.702879, whole genome shotgun sequence.
ACCESSION   DBMDUH010000118 DBMDUH010000000
VERSION     DBMDUH010000118.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 8367)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8367)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8367
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3826_length_8367_cov_4.702879"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /locus_tag="ACZPCG_09905"
     CDS             complement(<1..1073)
                     /locus_tag="ACZPCG_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="HIR1285405.1"
                     /translation="MLVYLICYIVSFILAWQHIYVLSGLVLLGAALWLYIKDYMRTGN
                     LIHLRGLFCLFFVGGQGISCFKLSRLQTDWALRTWISLFLAVVFFWTVFEILTRLFDG
                     WSEKDMQSVYRFFSSGESPLQAKRILISMSVITAVSLLAFLFEAWRLGFVPMFSYGVP
                     HAYSEFHISGVHYFTVSCVLVPSLSVVYSVMTARRGRGLTRDRGFWLAVVLDVVALSI
                     PLLCVSRFQLILAAGMAVFTYISMTGSFSLIHAGILIVLLIPAYLILTVLRSHSVEYL
                     NGIFEMKNSHMPIFITQPYIYIANNYDNFNCLVEQLGRHSFGLKGLFPLWALTGLKFL
                     FPSLVNFPLYVTKEELTTVTLFY"
     gene            complement(1073..2794)
                     /gene="ptsP"
                     /locus_tag="ACZPCG_09910"
     CDS             complement(1073..2794)
                     /gene="ptsP"
                     /locus_tag="ACZPCG_09910"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HIR1285406.1"
                     /translation="MYKGTNASDGIGIGTAVIVEEAELIITRREVNDTEAEVQRFKGA
                     LETTIAETQKMAEDLAARVGEKEAEIMQGHMMLLSDPMLTGEIENSIQNDKVNSEFAI
                     ENVCNMYADMFASMGDELMQQRATDMRDIKVRMQRTLMGLQSVDVSALPAGTILVAKD
                     LTPSMTAGINPANVTGIVTELGGRTSHSAILARALEIPAVVAVNGLLDEVKDGDTVVL
                     DGSAGEVYVNPEAEVKAAYEAKREQFLMDKKELEKYIGQPSVTKDGVQVEIVANIGKP
                     EDIDKVLQYDAEGIGLFRTEFLFMDRTAMPTEEEQFEAYKKVAAAMNGKPVIIRTLDI
                     GGDKEIPYMGLEKDENPFLGYRAIRLCLDRKEDIYKPQLRALLRASAFGNIRIMIPLV
                     TCIDEFREAKALIEEIKKELDEKGIAYKKDIQVGIMVETAAASLIADIFAKEADFFSI
                     GTNDLTQYTMSVDRGNKKISYLYSTFNPAVLRSIRHIIACGRETGIMVGMCGEAASDP
                     MMVPLLLAFGLNEFSMSASAVLRTRKLVTGFSIKELQAVAEKAMSFATAAEVEAYMKE
                     FMEKMTD"
     gene            complement(2877..3140)
                     /locus_tag="ACZPCG_09915"
     CDS             complement(2877..3140)
                     /locus_tag="ACZPCG_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HIR1285407.1"
                     /translation="MVNEATIIKNPSGLHLRPAGILCKEALKFKSKISFRVRNVTANA
                     KSVLSVLGACVKCGEEVEFICDGSDEEEALKAIIRLVDDRLGE"
     gene            complement(3177..4484)
                     /locus_tag="ACZPCG_09920"
     CDS             complement(3177..4484)
                     /locus_tag="ACZPCG_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HIR1285408.1"
                     /translation="MEKKLINNLKLKILSVFIAFFVWLAVVNISNPDITATQIVSLDV
                     INESVLTSSKKTYELLDDRSTVTVSYKVRTLDAGGVSPSDFRAYIDLADMYEPTGAVP
                     VKIEVKNNRVDSVEAKPGVIRVRTEDLQEKPFELTPKYVGKAEEGYRQGSVSLSPSKI
                     VVSGPQSLVGQISSVGIEIDLSGATEDLTGTRPIKCYDANGEEIALDERVTLSRTEAD
                     YDQSILKVKNLGLNFETEGRVAEGYRYTGIESSINSVEVVGLKSDLAGINSITIPKTE
                     LNMDGADSDRKVNIDLKQYLPDGVSLAGEESQITVTIKVEPLETKDIVLGADAVRQVG
                     ASSHYSYQFDRDQITVTIKGLAEDLDQLTEESLEAEVDVSDMQPGTHTAEVTFQLGAA
                     YELVSCDPLQIIVHDRTEESKESMETESQAETEEESASASEQS"
     gene            complement(4462..5349)
                     /gene="cdaA"
                     /locus_tag="ACZPCG_09925"
     CDS             complement(4462..5349)
                     /gene="cdaA"
                     /locus_tag="ACZPCG_09925"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HIR1285409.1"
                     /translation="MDIFLEVLDKLSSWLSLLPRISAKNIVEIAIISFLVYELLVWIK
                     NTRAWTLLKGLLVIFAFSVVVVLFKLDTILWIIKNITAIAVTALLVVFQPELRKALEQ
                     LGSQNIITNFLPVDSSGKEGSGFNERTVNELVRATFEMAKVKTGALMVIERGTSLKEI
                     ERTGIEINGLISSQLLINIFEHNTPLHDGAVVIRGNRVAAATCYLPLSDNMSINKALG
                     TRHRAAVGVSESTDSLTIVVSEETGRVSVAQEGHLQTVSDAETLRTILSGIRETAEPS
                     GSRFRLWKGRLINGKKADK"
     gene            complement(5383..6354)
                     /locus_tag="ACZPCG_09930"
     CDS             complement(5383..6354)
                     /locus_tag="ACZPCG_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008397452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1285410.1"
                     /translation="MTGLLLWKEHLKGFYQKYNQLLHSLIRFLFTLCTVLSINSTVGY
                     MAKLKNPLVVFMVCLVGTLLPNCMLVFLIGGFLAVHLYAVSVEMALIAVLVMMIIGIL
                     YYGFKPGDSWLMVLTPLAFLFKIPYAVPLLVGLGGSLSSVIPVGCGIFFYYLIMYVKQ
                     NAGVLTGEAAVDMVQRYSQIVRSVFLNQTMTVMIATCAVGILVVYLIRRLSIDYAWLA
                     AIVTGSVAELVVIFIGDFVFGVSAAPLEMVVGMLVSAALAALYNFFIFSVDYSRTEYV
                     QFEDDDYYYYVKAVPKMTMSTTDVRVKKINTHRRSAREREPGGTRQP"
     gene            complement(6351..6782)
                     /locus_tag="ACZPCG_09935"
     CDS             complement(6351..6782)
                     /locus_tag="ACZPCG_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285411.1"
                     /translation="MLNEEKIRYMTELAIFEKNEGRKIFPVNRFFRSDYVGGQMFRGF
                     FGYTFSFLLIVFTWAMYRLDVILGGAGPDTVLGWIKNGTFAYVGGLVLYLAVTWKISS
                     RRYEIAARSQTMFTARLKHLIRRYGKDQPDTGREKRGGRAI"
     gene            complement(6895..>8367)
                     /locus_tag="ACZPCG_09940"
     CDS             complement(6895..>8367)
                     /locus_tag="ACZPCG_09940"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIR1285412.1"
                     /translation="EAEKREEAAYGELEGLLEETSYQFPLLWTGCWGDCLTGLLVAEG
                     VWDKEQCSRVLREISGFVGKMAQYRLMQEKYSYVMTHDRESGLLNYQSYIRCLSHENQ
                     EIYSSMGVAGIHIWDFKGYNQRYGHAGGDRLLAQLGEKIREIFKGADCYRISGAGFLI
                     RFADVTKDRFEEQGRRFQMEAETLCPGQILCEYVWTAQVQAPMKLQQQVEEKLQIAMN
                     QRSTRAYGGRTPAAARMLSELKQAIKRGGFTAFFQPKADLKTGRICGAEALVRYQHGR
                     KGVITPDKFLPEIEKAGLIRYIDLFVLEESCRLMRQWLDEGWEGFPVSLNYSRITILE
                     PGILEETCGITDRYRIPHRLISIEITETISAVDNISLKAITTAFTEAGYGISLDDFGT
                     EYSNIHVLYDLPIKILKIDRSIVTDMFHNPKAGIVAQSVFAACKSLKIRTVAEGVETK
                     EQADILKEMGCDMIQGYYINKPLPGDCFKETCLSFSDIQK"
BASE COUNT         2155 a         2281 c         1723 g         2208 t
ORIGIN      
        1 tcgtaaaata aggtgaccgt cgtcagttcc tcttttgtta catataatgg aaagttcacg
       61 agagacggaa acaggaattt aaggcctgtc agcgcccata atggaaacag tcccttgagt
      121 ccgaagctgt ggcggcccag ctgttctacc aggcagttga agttgtcgta attattagca
      181 atgtagatat aaggctgggt gatgaaaatg ggcatatggc tgtttttcat ctcaaagatg
      241 ccgtttaaat attctacact gtggcttctt aatacggtga ggatcaggta ggccgggatt
      301 aaaaggacga tcaaaattcc tgcgtgtatc agactgaagc ttccggtcat agagatatag
      361 gtgaataccg ccatgcctgc tgcaaggatc agctgaaatc tggatacaca taaaagcggg
      421 atagaaagag ccaccacatc caaaaccact gccagccaaa agcctctgtc tcttgtaagt
      481 cctcttcccc gtctggctgt cattacgctg taaaccacgg aaagactggg aaccagtacg
      541 caggatacgg taaagtaatg aactccgctg atgtggaact ctgaataggc gtgaggtacg
      601 ccgtaagaaa acatgggaac aaagcccagt ctccaggcct caaataaaaa ggccagaagg
      661 gatactgcag tgatgacgct catggaaatc aggatgcgct ttgcctgcag aggcgattcc
      721 ccggaggaaa aaaagcggta aactgactgc atgtcctttt ccgaccagcc gtcaaaaaga
      781 cgggtgagta tctcaaagac cgtccagaaa aaaacgacag ccagaaaaag gctgatccag
      841 gtcctaagcg cccagtcagt ctgaagcctg gaaagcttaa agcaggagat cccctgtccg
      901 cccacaaaaa agagacagaa cagtccccga agatggatca ggtttcctgt tctcatataa
      961 tcctttatat acagccacag agctgctcct aagagcacaa ggccggacag cacataaatg
     1021 tgctgccagg ccaggataaa acttacaatg taacaaatga ggtaaactaa catcagtcgg
     1081 tcatcttctc cataaattct ttcatataag cttccacttc tgccgctgta gcaaagctca
     1141 ttgccttctc tgccactgcc tgcagctcct taatgctgaa gcctgttacc agctttctgg
     1201 tgcgcagcac tgcagaggcg ctcatactga actcatttaa gccaaaggca agcagcagcg
     1261 gaaccatcat gggatcgctg gcagcctcgc cgcacattcc taccataatg cctgtttccc
     1321 gtccgcaggc aataatatgg cggatacttc taaggacagc cggattaaag gtggagtaaa
     1381 ggtaggaaat ctttttattt ccgcggtcta ccgacatggt atactgggtc aggtcattag
     1441 ttccgatgct gaagaaatct gcttcctttg caaaaatatc agcgatcagg gatgctgccg
     1501 cagtctccac catgatgcct acctgaatat ccttcttata ggcaattccc ttttcatcca
     1561 gttccttttt gatctcttcg atcagggcct ttgcctctct gaactcatcg atacaggtta
     1621 caagaggaat catgatgcgg atgtttccaa atgcactggc tcttaaaagg gcgcgcagct
     1681 gaggcttata gatatcctcc ttacgatcca ggcataaccg gatagcacgg tagccaagga
     1741 atggattttc atccttttca agtcccatat aaggaatttc cttatcgcct ccgatatcca
     1801 gggtacggat gatcaccggc tttccgttca tagcggcagc taccttttta taagcctcaa
     1861 actgctcctc ctctgtaggc attgcagtgc ggtccatgaa taagaactcc gtacggaaca
     1921 gaccgattcc ctctgcgtca tactgaagca ccttatcaat atcctcaggc tttccgatat
     1981 tggctacaat ctcaacctgt acaccatcct ttgtcacaga gggctgtccg atatattttt
     2041 ccagctcctt cttatccatt aaaaactgct ctctctttgc ctcgtaggca gcctttacct
     2101 ctgcctccgg atttacataa acctctccgg cgcttccgtc aaggacaacc gtgtctccat
     2161 cctttacctc atctaacagg ccgtttacgg caactaccgc aggaatctcc agggctcttg
     2221 caagaattgc actgtgggag gtacgtccgc caagttctgt tacaataccg gttacattcg
     2281 ccggattgat gcctgctgtc atagacgggg tcagatcctt tgccaccagg atcgttcctg
     2341 ccggaagggc agatacgtcc acagactgaa gtcccatcag ggtacgctgc atacgcacct
     2401 taatgtcccg catatcggtc gctctctgct gcatcagctc atcgcccatg gacgcgaaca
     2461 tatctgcata catattgcag acattttcaa tggcaaactc actgtttacc ttatcattct
     2521 gaatagaatt ttcaatctcc cctgtcagca tcgggtcaga taaaagcatc atatgaccct
     2581 gcatgatctc ggcttccttt tcacccacac gggcagccag atcctcagcc attttttggg
     2641 tttcagcaat ggttgtttcc agcgctccct taaaacgctg cacctctgcc tctgtatcgt
     2701 ttacttctct ccttgtaatg ataagctctg cttcctccac aatcaccgca gtaccgatgc
     2761 cgataccgtc agaagcattt gttcccttat acatatgtat ttcctcctta atattttgaa
     2821 atcccggccc ataccggtat ttccttattt atactcacaa cctttggttg ccgtcatcat
     2881 tctcccagac ggtcgtccac cagacggata atggctttaa gagcctcttc ctcatcggaa
     2941 ccatcacaga taaattccac ttcctcaccg catttgacac aggctccaag aacgcttaaa
     3001 acactctttg cattggccgt aacgttgcgg accctgaagg atatttttga tttaaacttt
     3061 aacgcttcct tgcacaaaat tcctgccggc cttaagtgaa ggccggatgg atttttaatt
     3121 atagttgcct catttaccat ataacttcac tcctgtctta tccataatac acgctgctat
     3181 gactgctcgg aagcagaggc gctttcttcc tctgtctctg cctggctttc cgtttccatg
     3241 ctctcttttg attcctctgt ccggtcatgg acaatgatct gaagaggatc acagcttacc
     3301 agctcatagg cggctcccag ctggaacgtt acttctgccg tatgggttcc gggctgcata
     3361 tcgcttacat ccacctctgc ctccaggctt tcctcggtca gctgatccag atcctccgcc
     3421 agccccttga tggtaaccgt gatctgatcc cggtcaaact gatagctata gtggctggat
     3481 gcacctacct ggcggactgc atcggctccc agcacaatat cctttgtttc aagaggctct
     3541 accttgatgg taaccgtaat ctgactttcc tctcctgcaa gagatactcc gtccggcaga
     3601 tactgtttca gatcaatatt gaccttgcgg tctgaatcag ctccgtccat attcaactct
     3661 gtcttcggaa tggtaatgga attgattccc gccagatcgg atttaagtcc gactacttcc
     3721 accgagttaa tgctggactc aatccctgta tagcggtatc cttctgccac acggccctct
     3781 gtttcaaaat tcaatcccag attctttacc ttgagaatag actgatcata atccgcctcc
     3841 gtccggctta aagtaacccg ttcatccagg gcaatctcct cgccattggc atcgtaacat
     3901 ttaatgggcc tggtaccggt aagatcctcc gtcgccccgc ttaagtcgat ttcaatcccc
     3961 acgctgctga tctgtcctac cagagactgg gggccgctta caacgatctt agacggtgac
     4021 aaactgacgc ttccctggcg gtagccttcc tccgcctttc ctacatattt aggcgtaagc
     4081 tcaaaaggct tctcctgcag atcctctgtc cttacacgga tcactcccgg cttggcctct
     4141 accgaatcca cacggttatt tttaacctct atcttgacag gaactgcacc tgtaggctca
     4201 tacatatctg ccagatcaat ataggctctg aaatctgaag gagaaacacc tcctgcgtcc
     4261 agagtccgga ccttgtagga aacagtaacc gtactcctgt cgtctaaaag ctcataggtc
     4321 tttttactgg aggtaagcac agattcattg atcacatcca gagacacgat ctgggttgct
     4381 gtaatatcag ggtttgatat atttaccaca gccagccata caaagaaagc gatgaatacg
     4441 gaaagaattt tcagttttaa attatttatc agcttttttt ccattgatca gccttccctt
     4501 ccataaccta aaccgactgc cagacggttc ggctgtttcc cggatcccgg acaggatcgt
     4561 tctgagggtt tccgcgtcag ataccgtctg aagatgtcct tcctgagcaa cggaaacacg
     4621 tcccgtttcc tctgatacaa caatcgtcag gctgtctgta ctttcactaa cgcctactgc
     4681 cgcacggtgg cgtgttccca gagccttgtt gatggacata ttatccgaca gaggcaggta
     4741 gcaggtagct gctgctaccc gatttccccg gatcaccact gctccgtcat gaagaggggt
     4801 attgtgctca aatatattga tcagaagctg gctgctgatc agtccgttga tctcaattcc
     4861 cgtacgctca atctccttta aggaggtgcc gcgttcaatc accatcagag ctcccgtctt
     4921 tacctttgcc atttcaaagg tggcgcggac cagctcattg accgtacgct cattaaatcc
     4981 gcttccttct ttaccggaag agtctaccgg aagaaaattg gtaataatat tctggcttcc
     5041 cagctgctcc aaagcctttc tgagttccgg ctgaaataca actaaaagag ccgttaccgc
     5101 aatggctgta atatttttga tgatccagag aatggtatcg agcttaaaca gcaccacaac
     5161 cactgaaaag gcaaaaatca ccaaaaggcc ttttagaaga gtccaggctc tggtgttttt
     5221 gatccagacc aaaagctcgt agacaagaaa agaaataata gcgatctcca ctatattttt
     5281 ggctgagatc ctgggcagca gagacagcca ggatgacagc ttatccagca cttccagaaa
     5341 aatatccatc ttctatcgcc ctcctctcac aaatatcata tgttacggct gccttgttcc
     5401 tcccggctca cgctctcttg cgctccgcct gtgcgtattg atctttttta cccgcacatc
     5461 tgtggtgctc atagtcatct ttggaacagc ttttacatag tagtaatagt catcgtcctc
     5521 aaactgcaca tactccgtgc gggagtagtc tactgaaaaa ataaagaaat tatacaaagc
     5581 cgccaaagcc gccgacacca gcatccccac aaccatttca agaggtgctg ccgacacgcc
     5641 aaacacaaaa tcaccgataa agatcaccac aagctctgct acagaacctg tcacaatggc
     5701 tgccagccag gcatagtcaa tggaaaggcg gcggatcagg tataccacaa gaatacctac
     5761 cgcacaggta gcgatcatca ccgtcatggt ctgatttaaa aatacggacc gcacaatctg
     5821 gctgtagcgc tgtaccatgt ccacggcggc ttctcctgtg agaacgcctg cattctgctt
     5881 tacatacatg ataaggtagt aaaaaaagat gccgcagccc acagggatca cggaacttaa
     5941 gcttcctccc agtcctacca gaagcggaac cgcataaggg attttaaaca gaaatgccag
     6001 cggcgtaaga accataagcc agctgtctcc cggtttaaag ccataataaa ggatgcctat
     6061 tatcatcatc acaagaaccg cgatcagcgc catttctaca gacaccgcat acagatgaac
     6121 tgccaaaaat ccccctatga gaaataccag catacagttg ggaagcagcg ttccgaccag
     6181 gcagaccata aaaaccacca gtggattctt aagttttgcc atatatccaa cagtagaatt
     6241 gatgcttaaa acagtgcaca atgtaaacag aaagcggatc agcgaatgga gcagctgatt
     6301 atatttctga taaaatccct tcagatgctc tttccagagc agcagtcctg tcatatagct
     6361 cttcctcccc gtttttctct gcctgtatcc ggctggtctt ttccatatcg tctgatcaga
     6421 tgttttaagc gggctgtaaa catcgtctgg ctccgtgcgg ctatctcata gcgccgggag
     6481 ctgatcttcc aggtaacggc aagatataaa accagacctc ccacatatgc aaacgtcccg
     6541 tttttaatcc agccaagaac cgtgtcgggc ccggccccgc ccaagatcac atccaatctg
     6601 tacatcgccc aggtgaaaac gatcaaaagg aagctgaagg tatagccgaa aaagcccctg
     6661 aacatctgac cgcccacata atcgctccta aaaaaacggt ttacgggaaa aatctttctg
     6721 ccttcattct tttcaaatat ggccagctct gtcatatagc ggatcttctc ttcattcagc
     6781 ataggaacgc tccttattat atgcccggaa atgatttaca ggcacaggat tctcaaaata
     6841 tgtcaattca gtataccaca actttttata ttttgcgaca ctttaaatga acttttattt
     6901 ttgtatgtcc gaaaaggaca gacaggtttc tttaaagcaa tcgcccggaa gcggcttatt
     6961 aatatagtaa ccctggatca tatcgcagcc catttctttt aaaatatctg cctgttcctt
     7021 tgtctccacg ccctcggcca ccgtacggat cttaaggctt ttgcaggccg caaatacgct
     7081 ctgggccaca attcctgcct ttggattgtg gaacatatct gtgacaatgc ttcggtctat
     7141 tttcaggatc ttgatcggga gatcataaag cacatggata ttgctgtact ccgttccgaa
     7201 atcgtccagg gaaataccat agccagcctc tgtaaatgct gtggtaatgg ccttcaggct
     7261 gatattgtct actgcgctga tagtttctgt aatctcaatg gaaataagcc tgtgcggaat
     7321 acgataacgg tctgtaatac cgcaggtttc ttctaatatc cccggctcta aaatcgtaat
     7381 tctggaataa ttaagagaaa caggaaatcc ttcccagcct tcatccagcc actggcgcat
     7441 caggcggcag ctctcctcca gcacaaaaag gtcaatatag cggatcagcc cggccttttc
     7501 aatttccggc agaaatttat ccggcgtaat tacacccttt cttccatgtt gatagcgcac
     7561 cagcgcctct gctccacaga tacggcctgt ttttaagtct gccttgggtt ggaaaaaagc
     7621 agtaaacccg cctcttttta tcgcctgttt aagctcagaa agcattcggg ctgcggcagg
     7681 cgtccttccg ccataggccc tggtacttct ctggttcatg gcgatctgca gtttttcctc
     7741 cacctgctgc tgaagcttca tgggcgcctg aacctgagct gtccacacat attcgcagag
     7801 gatctgcccc gggcacagcg tttctgcttc catctgaaac cgcctgcctt gttcctcaaa
     7861 ccggtctttt gttacatctg caaaacggat cagaaagccc gctccactga tgcggtagca
     7921 gtccgccccc ttaaaaattt cccgtatctt ctctcccagc tgtgctaaaa gccggtcgcc
     7981 tcctgcatgg ccatagcgct gattatatcc cttaaaatcc cagatatgga tccctgctac
     8041 tcccatggat gaatagattt cctgattttc gtggctcagg caccggatat aggactgata
     8101 atttaaaagc ccggattccc tgtcatgggt cataacatag ctgtattttt cctgcatcaa
     8161 acggtactgg gccatctttc ccacaaagcc ggaaatttcc ctcaatactc tggaacactg
     8221 ttccttatcc catacccctt ctgctacaag caggcctgta aggcaatctc cccagcagcc
     8281 agtccacaaa agcgggaatt gatacgatgt ttcttccagc aggccttcca gctccccata
     8341 ggcagcttcc tccctctttt ctgcttc
//
LOCUS       DBMDUH010000119         8314 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_3858_length_8314_cov_5.438208, whole genome shotgun sequence.
ACCESSION   DBMDUH010000119 DBMDUH010000000
VERSION     DBMDUH010000119.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 8314)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8314)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8314
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3858_length_8314_cov_5.438208"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1112)
                     /locus_tag="ACZPCG_09945"
     CDS             complement(<1..1112)
                     /locus_tag="ACZPCG_09945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1285413.1"
                     /translation="MEQNQRFLLEKPVFPLLVSMAVPVMLSMLINSLYNVVDSIWVSR
                     LGTDAITAVSLAFPLQNIIMALGVGLGVGVSSLISMSLGAGNRNKADHLASAGLSLAL
                     IHCLLFIALGIFITRPFLGMFTDNPQIFEWACSYTFIVLCLSSGQVLQMYMEKLFQGV
                     GRMKTTMFLMASGCLINIVLDPILIFGWFGAPAMGVSGAAVATVIGQFGALLLYILVY
                     LKTNLGVSIRPRLMNLNRELIRSIYSIAIPSSLLLAMPSLLTGILNGILTTFGSVYVA
                     AFGLYFKLQTFVNLPCNGLLQGMRPIIGYNYGAERHDRVRSTIRCSLGLVTVVTLTGT
                     IASIFFPGPILRIFDADPELMQYGTEALRMIGPSFL"
     gene            complement(1271..1343)
                     /locus_tag="ACZPCG_09950"
     tRNA            complement(1271..1343)
                     /locus_tag="ACZPCG_09950"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1308..1310),aa:Lys,seq:ctt)
     gene            complement(1394..2323)
                     /locus_tag="ACZPCG_09955"
     CDS             complement(1394..2323)
                     /locus_tag="ACZPCG_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006771009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR1285414.1"
                     /translation="MIYFTAALKTPSIPTLEETQAIVQEVHEDLQQLKPNAILETIRS
                     WAPGLLSLSYRILFAAIIIIIGNRIAFYLRRFLKRTFDRMNIDLSLSKFLISLANAIT
                     YAIVIFMALEKIGIPSTSIIALLGSATLAIGLSLQESLGNFAGGILILVMRPFGIHDY
                     IVSEGVEGQVLNIGLVYTTLLTVDNRRITIPNGTLSNAVITNVTAQEKRRIDLTIGIS
                     YSSDLKQAKAILERIYREDPQIIQDEPITVYVSELAESSVIIGSRGWAATEDYWSARW
                     RIIEKTKLEFDAAGIEIPYNQMDVNIFQKTIDK"
     gene            complement(2405..3475)
                     /locus_tag="ACZPCG_09960"
     CDS             complement(2405..3475)
                     /locus_tag="ACZPCG_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1285415.1"
                     /translation="MINHKITCQIIEKLQERFSLPISICDLSGRTIASTEPSCIGEMN
                     LLAIESLNLNAKVTTAPGTYFQNASTAVPLRLQGSRIGAVVIEPSSSANLHMAELLGN
                     TIELLYEELLSFQKRQNQSQERSQFLFECLHQQAPYTENFIKRGEFFGIQLKGKQTLI
                     LMERKTDDQFTSISMLQNLLDEHDIILPLSENQVLLVLKENVTFEKKYHRILSAAADC
                     HMGICSGEMHLNTAYQAALESLKLGKALFPDEHLHSYDKMKLAIALSKTAVPGLEQEF
                     QQLCRQGKNAQLAETAVAYLRLNGDIQKICEHLHIHRNSIPYRLHRIQEICGRNLTNS
                     YDMLCLYASYICHIKKQIQEKS"
     gene            3717..4790
                     /locus_tag="ACZPCG_09965"
     CDS             3717..4790
                     /locus_tag="ACZPCG_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR1285416.1"
                     /translation="MRYVDCASETHNERAFCEYMERELTELGISFERQELDHQIVTDG
                     WNILAKVPGKPDKKPFLFVFHLDTVVPGNNVESYIENGRIQSRGNSVLGADGKLAIAV
                     VMEAVSRLLRAGEMNRPVEMLFTVCQELGLLGAKYADYSRIDSEEAIVIDHYVTGEVL
                     MCTPSRMYLHVELIGQEAHVIRDPELGINALKTAAEIIHQIPTGQVSKDQTINVFDLV
                     SLSQSNAIPKYARFDVEIRSFGQELLDRTNAFLQKIIKDTAAQMGCRCSVREEMDLPE
                     TDYSENKDMLKRLERIYEMTGLQMRPARSFGILDATCTNRLGIRTIPIGMNIYYSHST
                     REYVEVKDIKIMLDLAENILRYF"
     gene            4810..6207
                     /locus_tag="ACZPCG_09970"
     CDS             4810..6207
                     /locus_tag="ACZPCG_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HIR1285417.1"
                     /translation="MKNEKKKTGKALNPFIPLVLMVLACAIASYFVVPGAYDRETVNG
                     VTRVMADSYHAVERTPISFFNLFRSIPEGLTATANMMFCVMIIGGIVEIYKRTDTVGA
                     AINSVLRVSEKLSSQMIIAVVMIVFFIFGGILGWSEHIIPFVPIIISLAISLGYDSLV
                     GMAISGFACLISFAVAPFNVYTVGISHTIAELPMFSGWELRFAALGCVWALSLFWVMR
                     YAKKVKANPSKSLVKDVDTSSLRIPVDPSLKFDLPKKVSFVVLGVSILFTIYGILKLS
                     WSYTEMATTFMVGGVIVAIINRVKMEDAINMILDGMRGAFVGALIIGVARAVQWTMTN
                     GGLIDPLVHGLSSLMGSASAYVSTVGMFVVNFFINALIPSGSGQATAVMPIMVPLADM
                     LNITRQTAVLAFQFGDGISNTFWFTNGTLLIYLSLGKVPLKSWYKFILPLHGMFLVLQ
                     LVFLFIAVQTGYGPF"
     gene            6239..7405
                     /locus_tag="ACZPCG_09975"
     CDS             6239..7405
                     /locus_tag="ACZPCG_09975"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 metallopeptidase family protein"
                     /protein_id="HIR1285418.1"
                     /translation="MNVLEEVKKYKDYAVAMRREFHRHPELSWQETETAARIRRELDE
                     MGIPYEEVVGTGTIATLKGKKDHPVIGLRCDIDALPIKEVKELPYRSENDGVMHACGH
                     DGHISMLLTAAKVLSDHRDELNCTVKLIFQPAEEKTNGALKMLETGRVGHLDTVCGMH
                     IFPFLDAGTISVDPGPRYTSAAFMNITIKGRSGHGAMPHYAVDPIYVGAKVVDALQSI
                     ASRETNAMDTVVVSICTFHSGTMANIFAETAELSGTVRTFNPELRKQLPGMIERIIKS
                     TCEAYRADYEFDYYCDIPATINDEHCSEIAAGSVKKLLGEEGLMKYAGTPGGEDFSYF
                     LERFPGVYAFVGCRNESKGCSYSLHHERFDLDEDALVNGAAFYVQYLLDAQEQF"
     gene            7512..>8314
                     /locus_tag="ACZPCG_09980"
     CDS             7512..>8314
                     /locus_tag="ACZPCG_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009536817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR1285419.1"
                     /translation="MNTRFYNIRLLTMDGTMDVVPGEVWVEGNRISYAGPSAFMGDAN
                     TDAITPPVWDREIDGNGNLLMPGFKNAHTHSAMTFLRSYADDLPLMDWLHTQVFPMEA
                     KLQPGDAFWLSRLAVMEYLTSGMTAAFDMYPWPEEMARACVESGFRTVLVSGLNDFVS
                     SPEGLEEDYLKFNSYDPLVSYQLGFHAEYTTSRKILEAVADLAHKYKAPVFTHNSETK
                     GETEGCLERYGMTPTAFLDSLGMFEYGGGGYHCVHITDEDMDIFKKHDMT"
BASE COUNT         2313 a         1619 c         2162 g         2220 t
ORIGIN      
        1 atcaaaaaac tggggccgat catgcgcagg gcttctgtcc catactgcat cagctccgga
       61 tctgcatcaa aaatacgcag aatcgggccg ggaaagaaaa tggatgcaat tgttccggtg
      121 agtgtaacaa cggttaccag gccaaggctg cagcgaatgg tactgcggac cctgtcatgg
      181 cgttcggctc catagttata cccgataatg ggacgcattc cctggagaag gccgttgcag
      241 ggaaggttta caaaggtttg gagtttaaaa tacagtccaa aggctgctac atagacgctt
      301 ccaaaggtag tcaggattcc atttaaaatg ccggttaaca gggaaggcat ggctaaaagc
      361 agactggatg gaattgcaat actataaata gaacgaatca gttcgcggtt cagattcata
      421 agtctgggcc gaatggatac gcccagattg gtttttaaat acacaagaat atatagaagc
      481 aacgccccaa actggccgat cactgttgca acggcagcgc cggatacacc catggcggga
      541 gcgccgaacc agccgaaaat aaggatcgga tctaaaacaa tgttgatcag acagccgctt
      601 gccatgagaa acatggtggt tttcatccgc cctacaccct gaaagagttt ttccatgtac
      661 atttgcagaa cttgaccgga agagagacag agcacaatga acgtatagct gcaggcccat
      721 tcgaatattt ggggattatc tgtaaacatc ccaagaaatg gtcttgtaat aaaaatgccc
      781 agagcaataa ataacagaca gtgtatcagg gccagagaga ggccggcaga tgccagatga
      841 tcggccttgt tcctgtttcc tgctccaaga ctcatggaaa ttaaggagct gacacctacg
      901 cccaagccca ctccaagagc catgatgata ttctgaagag gaaaggctag tgaaacggca
      961 gtgatggcat ctgtgcccag tctcgatacc cagatactgt ctacaacgtt atagagagaa
     1021 ttgatcagca tggacagcat cacaggcact gccatggaaa caagaagagg aaaaaccggc
     1081 ttttccagca aaaaacgttg attctgttcc ataataaaac ctcctgtaag gacaaaaaaa
     1141 gccctcccgc ctgttttgat gcgggaaggc ttgcatgcca ttgataaatg gaaaatgtat
     1201 aaaattatat gttcgcaata gaaaagtccg accccgcagc ctctaatatc tgcaaaatca
     1261 gacctctcag tgcgcctcca gggactcgaa ccctggacaa atagattaag agtctactgc
     1321 tctaccagct gagctagagg cgcatgtcta tgtgttgttg tgtgacacga tagacattat
     1381 aggggataga aagttatttg tcaatagttt tttgaaaaat atttacatcc atctgattat
     1441 aaggaatttc aatgcctgca gcatcaaatt ccaatttggt tttttcgata atccgccagc
     1501 gggcgctcca gtaatcctct gtagcggccc atccacggga accaatgatt acagagcttt
     1561 cagcaagctc gcttacataa acagtaatgg gttcatcttg aatgatttga ggatcttctc
     1621 tgtaaatgcg ttccagaatc gcttttgcct gttttaaatc tgaagagtag ctgatgccta
     1681 tagtcagatc aatgcgccgc ttttcctgag ctgtcacgtt ggtaattaca gcatttgaca
     1741 gggtgccgtt agggatggtg attcgacggt tgtcaacggt gagcagggta gtgtatacaa
     1801 ggccgatatt taaaacctgc ccctctaccc cctcggatac aatatagtca tggatcccaa
     1861 agggccgcat gaccagaatc aggatacctc ctgcaaagtt tcccagactt tcctgcagag
     1921 aaaggccgat tgccagagtg gcggagccca gcaatgcgat gatggaggtc gatggaattc
     1981 ctattttttc cagtgccata aagataacaa tagcataggt aatggcattt gcgagagaaa
     2041 taagaaattt gctcagactc aggtctatgt tcattcggtc aaaggtgcgt tttaaaaacc
     2101 ggcggagata aaatgcaatt cggttgccaa tgataataat aatggcagcg aagagaatgc
     2161 gatagctgag tgataaaagt cccggagccc aggaacgtat ggtttctaaa attgcgttag
     2221 gtttcagctg ctgaagatct tcgtggactt cctgtacaat agcctgcgtt tcctccagcg
     2281 tagggattga aggggtcttc agggctgcag taaaatagat cattcaaaaa ttctcctgtt
     2341 tttaaatagt gtaaaatatt attataacat aaaagagcat actgttaaaa tacatcctgc
     2401 aaaattatga tttttcctga atttgctttt ttatatggca gatataggac gcatataagc
     2461 acagcatatc ataagaattg gtcaggttgc gtccgcagat ctcctgaata cggtgcaggc
     2521 ggtaggggat gctgtttctg tgtatatgga gatgttcaca gattttttgt atatccccgt
     2581 ttaaacggag ataggcaact gccgtttcag ccagctgggc atttttgcct tgtctgcaaa
     2641 gctgctgaaa ttcctgctcc agtccgggaa cggcagtttt ggataaggcg atagccagct
     2701 tcattttgtc atatgaatgc aggtgttcat ctggaaataa agccttccca agcttcagac
     2761 tttccagagc ggcctggtaa gcggtattca gatgcatttc ccccgaacag atgcccatat
     2821 ggcagtctgc ggcggcagag aggatgcggt gatatttttt ttcaaaagtt acgttttctt
     2881 ttaaaacaag aagaacctga ttttctgaaa gagggaggat aatatcgtgt tcatccagaa
     2941 ggttctggag catggagatg gaggtaaact ggtcatcggt ttttctctcc atcaggataa
     3001 gggtctgctt cccctttagc tgtataccaa aaaattcacc tcgcttgata aaattttctg
     3061 tatagggggc ctgctggtgg aggcattcga ataaaaactg gctgcgttcc tgggattgat
     3121 tctggcgttt ctggaaggaa agcagttctt cgtaaagcag ttctatggta ttgcccaaca
     3181 gttctgccat atgaaggttg gcagagctgc tgggttctat aacaacagcg cctatacggc
     3241 ttccctgaag gcggagagga actgctgtac tggcattctg aaagtatgtt ccgggtgctg
     3301 ttgtaacctt ggcgttcaga ttcagggatt caatggcaag aagattcatt tctccgatac
     3361 aggatggctc ggttgaggca atggtgcggc cgctgaggtc gcatatgctg atggggagcg
     3421 aaaatcgctc ctgcagtttt tctataatct ggcaggttat tttatggtta atcacgggtt
     3481 tcctccaatg atttttcata tattataata aaatggcgca aaaactgcaa gatatacgga
     3541 ataaattgtg caaatgcata aaaatagcgg gagatattac atggtttgga caatagtaaa
     3601 tctgatatgg aaatgttaaa ataaaatcac cttaaaaaag tgtgaagaaa aagccggagt
     3661 gaagtatttc agaaggaaag gagggaagag caatagacga gactgttttg cggtttatgc
     3721 gttatgtgga ctgtgccagt gaaacccata atgaaagggc gttctgtgaa tatatggagc
     3781 gggagctgac agaactgggc atttcgtttg aaagacagga gctggatcat cagattgtga
     3841 cagacggatg gaacattctg gcaaaagtac cggggaagcc agataaaaaa ccgtttctgt
     3901 tcgtatttca tctggatacg gtagttccgg gaaataatgt ggagagctat attgaaaatg
     3961 gacgtattca aagccggggc aacagtgttc tgggtgcaga cggtaaactg gctatagcgg
     4021 tggtgatgga ggctgtcagc aggctgcttc gtgccggaga gatgaaccgg cctgtggaga
     4081 tgctgtttac tgtctgccag gagctggggc ttcttggggc aaagtatgcg gattattcca
     4141 gaattgacag tgaagaggca attgttatcg atcattatgt gacaggagaa gtgctgatgt
     4201 gtactccgtc tcggatgtat cttcatgtgg agctgatcgg acaggaggct catgtgatcc
     4261 gggatccgga actgggaatc aatgcattaa agacagcggc agagattatt catcagattc
     4321 ctaccggtca ggtcagtaaa gaccagacca tcaatgtatt tgatctggtt tctctttcac
     4381 agtcgaatgc gattccaaag tatgcaagat ttgatgtgga gatccgaagc tttggacagg
     4441 agctgctgga tcgtaccaac gcgtttcttc agaaaatcat aaaggataca gcggcgcaga
     4501 tgggatgcag atgcagcgtg cgggaagaga tggatctgcc ggaaacggat tacagtgaaa
     4561 ataaggatat gctgaaacgt ctggagcgta tttatgaaat gaccggactg cagatgcggc
     4621 ctgcccgttc ctttggaatt cttgatgcaa cctgcacaaa ccggctggga atcaggacga
     4681 ttcctattgg gatgaacatt tattattcac acagcacgag ggaatatgta gaggtaaaag
     4741 acataaagat aatgttggat ctggcggaaa atatccttcg gtatttctga ccggagaaag
     4801 gggaaacaga tgaaaaatga aaaaaagaaa acaggcaagg cgctgaatcc gtttataccg
     4861 ttagtattaa tggttctggc ctgtgcaatt gcatcttatt ttgtagtacc aggggcgtat
     4921 gacagggaga cggtaaatgg agtaacccgc gttatggcag acagctacca cgctgtggaa
     4981 agaaccccca tctctttctt taatctgttc cgttcaattc cggaggggtt aacggcgaca
     5041 gccaatatga tgttctgcgt tatgatcatc ggcggtattg tagagattta taagcggaca
     5101 gatacggtag gagcagcaat taacagtgta ctccgtgtat cggaaaaact cagcagccag
     5161 atgattattg ctgtagttat gattgtgttc ttcatttttg gaggcattct gggatggagt
     5221 gagcatatta tccccttcgt tccgattatt atatctctgg ctatttcact gggatatgat
     5281 tcccttgtgg gaatggcaat ttccggcttt gcctgtttaa tcagctttgc ggttgctcct
     5341 tttaatgtgt atacggtagg tatttctcat acaattgcag agcttcccat gttctccgga
     5401 tgggagctgc gttttgcggc tctgggctgt gtatgggcgc tgagtctgtt ctgggtaatg
     5461 agatatgcca aaaaagtaaa ggcaaatcct tctaagagtc tggtgaagga tgtagatacc
     5521 tcttctttga gaataccggt tgatcccagt ttgaaatttg accttccaaa gaaagtttcc
     5581 ttcgttgttc tgggcgtatc tattcttttt acaatttatg gaattctgaa gctgagctgg
     5641 tcttatactg agatggcaac tacctttatg gtgggcggtg ttattgtagc tattattaac
     5701 agggtgaaaa tggaagatgc catcaatatg attctggatg gaatgagagg agccttcgta
     5761 ggagctctga ttatcggtgt tgcaagagcg gtacagtgga ccatgaccaa tggcggtctg
     5821 attgatccgc tggtacacgg actttccagt ttgatgggaa gtgcatccgc atatgtgagt
     5881 acagtcggta tgtttgtggt aaacttcttt atcaatgcat tgattccatc gggctccggc
     5941 caggcaaccg cagttatgcc gattatggtt cctctggcag atatgctgaa tattacccgc
     6001 cagactgccg tattggcttt ccagtttggt gatggtattt ctaacacatt ctggtttaca
     6061 aacggaacgc tgctgattta tctgtctctg ggcaaggtgc cgttaaagag ctggtataaa
     6121 tttattctgc cgctgcatgg tatgttcctg gttctgcagc tggtattcct gtttattgca
     6181 gtacagaccg gatatggtcc gttttaaaag aacgtaaaga ggggagagag aacttgctgt
     6241 gaacgtactg gaagaagtta aaaaatataa agattatgct gttgccatgc ggcgggaatt
     6301 tcaccgccat ccggagctga gctggcagga aacagaaaca gcggcccgga tacgcagaga
     6361 gcttgatgaa atggggattc cctatgaaga ggttgtggga acgggaacca tagcgacact
     6421 aaagggaaaa aaggatcatc cggtgattgg tcttcgctgt gatattgatg cgcttcccat
     6481 aaaagaagtg aaggaactgc cgtaccgttc tgaaaacgac ggggtgatgc acgcctgcgg
     6541 ccatgacggg catatcagta tgcttttgac agcggctaaa gtattgtctg atcacaggga
     6601 tgagctgaac tgtacagtaa agctgatttt tcagccggct gaagagaaaa caaacggcgc
     6661 actaaaaatg ttggagactg gaagagtggg acatctggat acagtttgtg gaatgcatat
     6721 tttcccattt ttggatgctg gaactatttc tgtagatccc ggtcccagat atacctcggc
     6781 tgcgtttatg aatatcacaa ttaagggcag aagcggtcac ggagcaatgc ctcattatgc
     6841 agtggatccc atttatgtag gtgcaaaagt agtggatgct cttcagagca ttgccagcag
     6901 ggaaacgaat gctatggata cagtggtagt aagtatctgt accttccatt ccggaaccat
     6961 ggccaatatt tttgcagaga cagcagaatt aagcggaact gtgagaacct tcaatccgga
     7021 gcttcgcaaa cagctgccgg gaatgattga gagaattata aagtctacct gtgaagcgta
     7081 cagggcagat tatgaattcg attattactg tgatattccg gcaacgatca acgatgagca
     7141 ttgcagtgag atcgcggcag gttctgtaaa aaagcttctg ggagaagagg ggctgatgaa
     7201 atacgctggc actccgggag gagaggactt ttcctatttt ctggaacgct tccccggtgt
     7261 gtatgcattt gtgggatgcc gcaatgaatc aaaaggctgc tcttattcac tgcatcatga
     7321 acggtttgat ctggatgagg atgcgctggt taatggagct gcattttatg tacagtattt
     7381 attagatgca caggaacagt tttagaaagg cagaaactgc tgtaccgcaa cgtggtatgg
     7441 cagtttttta tgtacatgaa aatcaagata tgatacaata ttgataaaaa ttaagaaata
     7501 ggagaagggc tatgaataca cgcttttaca atatccgtct tctgacaatg gatggaacta
     7561 tggatgtggt gccaggagag gtttgggtgg aaggaaacag gatttcctat gcaggcccgt
     7621 ccgcttttat gggagatgcc aatacggatg ctatcacccc tcctgtctgg gatcgtgaaa
     7681 tcgatggaaa tggcaatctt ctcatgccgg gctttaaaaa tgcccatact cattccgcta
     7741 tgacgtttct gcgttcctat gcggatgacc tgccgcttat ggactggctc catactcagg
     7801 tatttcctat ggaagcaaag ctgcagccgg gagatgcatt ctggctgtcc agactggctg
     7861 taatggagta tctgaccagc ggcatgacgg cagcttttga tatgtatccc tggccggagg
     7921 aaatggcaag agcctgtgtg gaaagtggtt tcagaaccgt tttagtaagc gggctgaatg
     7981 attttgtgtc atctcctgag gggctggagg aggattatct gaaattcaat tcctatgatc
     8041 cgttggtaag ctatcagctg ggctttcatg cagagtatac caccagccgg aagattctgg
     8101 aggcggtagc agatctggct cacaagtata aggctcctgt gtttacccac aattcagaga
     8161 caaagggtga aacagaagga tgtctggagc gctatgggat gacgccgact gcatttttag
     8221 attctctggg aatgtttgaa tacggcggag gcggttatca ctgtgtccac atcacagacg
     8281 aggatatgga tatctttaaa aagcatgaca tgac
//
LOCUS       DBMDUH010000120         8310 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_3859_length_8310_cov_6.292727, whole genome shotgun sequence.
ACCESSION   DBMDUH010000120 DBMDUH010000000
VERSION     DBMDUH010000120.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 8310)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8310)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8310
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3859_length_8310_cov_6.292727"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="ACZPCG_09985"
     CDS             complement(<1..470)
                     /locus_tag="ACZPCG_09985"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HIR1285420.1"
                     /translation="MAAQQQKQNYQRILEHTLEEIGKNGAVPTLLLHSCCAPCSSYVL
                     EYLSQYFEITVFYYNPNIYPETEYRERAREQERLICEMKTENPIHFLEGTYDPSVFYA
                     MARGHEKDLEGGERCFRCYELRLREGAAVAKQGGFDYFTTTLSISPLKNAQKLN"
     gene            complement(567..1709)
                     /gene="rpoD"
                     /locus_tag="ACZPCG_09990"
     CDS             complement(567..1709)
                     /gene="rpoD"
                     /locus_tag="ACZPCG_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HIR1285421.1"
                     /translation="MEKDDFLKKLEKLVEHAKSKHNTLDAGEINDFFTGDNLSPEQMD
                     QIYSYLENRNIDVVPVLDEAMLNADPALDDIGLDLDLDDDSFMKDAEEEEIDLDAVDL
                     LEGIGTEDPVRMYLKEIGTVPLLTADEELELAQRKADGDESAKERLIEANLRLVVSIA
                     KRYTGRGMSFLDLVQEGNLGLIKGVEKFDYTKGYKLSTYATWWIRQSVTRALADQART
                     IRVPVHMVETINKMSKMQRKLTLELGYEPSVTELAEALDMSEDKVMEIMQIAREPASL
                     ETPIGEEDDSNLGDFVADSNVVTPEGNVESVMLREHIDALLGDLKERERQVIVLRFGL
                     EDGHPRTLEEVGKEFNVTRERIRQIEAKALRKLRNPVRSKRIRDFL"
     gene            complement(1787..2680)
                     /locus_tag="ACZPCG_09995"
     CDS             complement(1787..2680)
                     /locus_tag="ACZPCG_09995"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008722807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIR1285422.1"
                     /translation="MKQMLFVFNPRSGKEQIRGSLLRILDIFTKDGYDIHVHVTQSQG
                     DASRIVKELGEGMDLILCSGGDGTLNETICGMLALKDQPPLGYIPAGSTNDFAVSLQI
                     PKDMERAALNVVNGAPFAVDVGSFCGDRNFVYIAGFGAFTEVAYATSQDRKNLLGHPA
                     YMLEALKSISSIKPYHMLVQWEEGELEEDFVFGMVTNTRSVGGFKNLVNQTVALNDGA
                     FEVLLIRMPKTPGDLSNIISHLFLKEEPNEYVYRFKTASICFQSAAPVDWVLDGEYGG
                     ARTQAQIGCLREKIRILVDRC"
     gene            complement(2677..3816)
                     /gene="mutY"
                     /locus_tag="ACZPCG_10000"
     CDS             complement(2677..3816)
                     /gene="mutY"
                     /locus_tag="ACZPCG_10000"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006780940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HIR1285423.1"
                     /translation="MCDGRLHFYDRLQILEEKDRSLEQRERLRAVEKPLLAWYASRAR
                     KLPWREDPKPYRVWISEIMLQQTRVEAVKPYFERFMEAFPEIRDLAQAEDDRLMKLWE
                     GLGYYSRARNLKAAAIQVMERFNGALPASYEALLSLPGIGSYTAGAIASIAYGIPVPA
                     VDGNVLRVVSRILADSSDIRKASVKTSMEALLKEVMPKDCAGSYNQALIETGALVCIP
                     GGEPKCRECPMESVCLTAQNGLWKKIPWKSPPKERKVEERTVFIIEYQEKAAIRKRPP
                     KGLLASLYELPNAEGKLSPKEAEAYLAVSEDDLVCLKPLPDARHVFSHIEWRMSGYHA
                     VLKEPWRELPDGVFMASREEIEKQYPLPNAFSAYREILFKKEDGQ"
     gene            complement(3809..4789)
                     /locus_tag="ACZPCG_10005"
     CDS             complement(3809..4789)
                     /locus_tag="ACZPCG_10005"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin--[biotin carboxyl-carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HIR1285424.1"
                     /translation="MKTEILKMLKETPDFLSGQQLCEGLGVSRTAVWKAIRQLEEEGY
                     AIEAVRNRGYRLVASPDVITAPELKTVLSTVWLGSQVNCFYETDSTNRQARKLAEEGA
                     PHGTLVAADSQSAGKGRRGRAWASPRGVGVWMSFILRPELSPSKASMLTLVAGMAVVA
                     GIRQISGLSPQIKWPNDAVVDGKKICGILTEMSTEEDSIRYVVVGIGINVNTESFPPE
                     ISDTATSLKLSLGHELKRAPLIGAVLKSFEQYYSRFMEYGDMTGLMDEYNEMLANRKR
                     QVRVLDPHGSYEGMALGIDKEGCLLVKREDDSVSAVISGEVSVRGIYGYV"
     gene            complement(4806..5114)
                     /locus_tag="ACZPCG_10010"
     CDS             complement(4806..5114)
                     /locus_tag="ACZPCG_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6637 family protein"
                     /protein_id="HIR1285425.1"
                     /translation="MQGQRGRNERRAGVFLDMAHLILGIAIVIFAVLSFISPEGNRML
                     FPLVFLLAALLNGIKGIFELKTCARDKKRFRLGIFQLLLGAGLGLLGVLSAVSLWFHG
                     "
     gene            complement(5163..6152)
                     /gene="argF"
                     /locus_tag="ACZPCG_10015"
     CDS             complement(5163..6152)
                     /gene="argF"
                     /locus_tag="ACZPCG_10015"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - L-arginine biosynthetic process
                     via ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HIR1285426.1"
                     /translation="MNLKGRNFLTLKDYTQEEILYLLDLAAELKDKKKKGILTDTLKG
                     KNVALIFEKTSTRTRCAFEVAAHDLGMGSTYLDPSGSQIGKKESIADTARVLGRMYDG
                     IEYRGFGQEIVEELAHYAGVPVWNGLTNEDHPTQMLADLLTIREHLGRLKGVKLVYMG
                     DARYNMGNSLMIACAKMGMHFTACAPKKYFPDPALVKECEAFAAESGGTITLTEDVAE
                     AVKGADVIDTDVWVSMGEPDEVWEERIRELSPYKVTKEVMDGAGSQAIFLHCLPSFHD
                     LKTKIGKEMGERFHVSELEVTDEVFESSQSVVFDEAENRMHTIKAVMLATLGE"
     gene            complement(6255..7394)
                     /locus_tag="ACZPCG_10020"
     CDS             complement(6255..7394)
                     /locus_tag="ACZPCG_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003525373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR1285427.1"
                     /translation="MKLQLDKGRRYGIVLEGGGARGAYQIGAWKALKEAGIQIQGAAG
                     TSVGALNGALICMDDFETAEKIWENIRYSQVMDVDDKRMEALREWNLKNGTAVRLSDL
                     LNGVKRLLKEGGFDITPLRELIRGAIDEAKIRTSDRDLYTVTYSLTEHQPLVVDIKEV
                     PPGQICDMLMASAYLIGFKPEKLGGKYYMDGARVNNVPVDVLIDRGYEDIIVLRIYGY
                     GVDTERRLQVPDGVRLYHVAPRQELGGILEFDRKKARRNMLLGYFDAMRMLYGLEGRI
                     YYLDAPESEEYYFNRLMAEVLELMDWCGMGAEHTENKEGGSLRIYMEELFPRLAAKLG
                     LKEKWDYRQLYFSLLEEAAKKYRVSRFRIYTPDQLLTVLRRRSWR"
     gene            complement(7391..7609)
                     /locus_tag="ACZPCG_10025"
     CDS             complement(7391..7609)
                     /locus_tag="ACZPCG_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HIR1285428.1"
                     /translation="MTGIAGWFFAYLFFMAVELFFRSLISLWFCAGALGAWIGALMGR
                     TAEEQLFVFLLVSVFSLWIIGAAARRRR"
     gene            complement(7666..>8310)
                     /locus_tag="ACZPCG_10030"
     CDS             complement(7666..>8310)
                     /locus_tag="ACZPCG_10030"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIR1285429.1"
                     /translation="VTPSFESQKENREWLHSYSYEIVTEEVMKAMADTKTPQGVLAVA
                     RQKKYTLEELLKPASSGTPLLLMLLENIQDPGNLGTILRAGEGAGVTGVIMSRDTVDI
                     YSPKVIRSTMGSVLRMPFLYTEDFTAVCKRLGREGVRLFAAHLKGMNNYDRQDYTGNV
                     GFLIGNEANGLTEAAAEAADCCIQIPMAGKVESLNAAVAASVLMFEAARQRRNI"
BASE COUNT         2001 a         2429 c         1697 g         2183 t
ORIGIN      
        1 tcattaagct tttgggcgtt ctttaaaggg ctgatagaga gtgtagtggt aaaatagtca
       61 aaaccgccct gttttgccac agctgctcct tcccggagac gcagctcata gcagcgaaag
      121 cagcgttctc ctccctccag gtccttttca tgtccccttg ccatggcata aaaaacggat
      181 ggatcgtatg ttccttccaa aaaatgaata ggattttctg tcttcatctc acagatcagc
      241 cgttcctgct ccctggcccg ttccctgtat tctgtttccg ggtaaatatt gggattgtaa
      301 taaaaaacag tgatctcaaa atactgtgac agatactcca atacatagct gctgcaggga
      361 gcgcagcagc tgtggagtaa aagagtcgga acggctccgt tttttccaat ttcttccaat
      421 gtgtgttcta aaattctctg ataattctgc ttctgctgct gtgcagccat aacaatttcc
      481 tcctttacag cagggacaaa cgcatcaata tcccgccacg cacaaaaggg ctgcttttgt
      541 gagcagccct ttttatttta tttcatttac aggaaatccc tgatgcgctt gcttcttacc
      601 ggattgcgca gcttgcgcag agccttggcc tcgatctgtc ggatacgctc tctggtaaca
      661 ttaaactctt ttcccacttc ctcaagggtt ctgggatggc cgtcttccag accaaaacgc
      721 aggacaatga cctggcgctc tctctctttt aaatcgccta agagggcatc gatatgttct
      781 cttaacatca cagactctac atttccctca ggggtcacta cgttgctgtc cgctacgaaa
      841 tcgcccagat tggagtcatc ctcttcgccg atgggcgtct ctaaggaagc cggttcgcgg
      901 gcgatctgca tgatctccat caccttgtcc tcagacatat cgagagcttc tgccagctcg
      961 gtgacagaag gctcatagcc cagctccaaa gtcagcttac gctgcatctt tgacatctta
     1021 ttgatcgtct caaccatatg caccggcaca cggatggttc tcgcctgatc agccagagct
     1081 cttgtcactg actgacggat ccaccaggtc gcatatgtac ttaacttata accctttgta
     1141 taatcaaatt tctcaacgcc tttgatcagc cccagattgc cttcctgcac cagatcaagg
     1201 aagctcatgc ctcttcccgt gtagcgcttt gcaatgctga ccacaagacg caggtttgcc
     1261 tcgatcagtc tctcctttgc agattcatct ccgtctgcct tgcgctgtgc aagctccagc
     1321 tcttcatctg ccgtaagaag gggtacggta ccaatctcct ttaagtacat acgaaccggg
     1381 tcttcggttc cgatgccctc cagaagatcc actgcatcca ggtcgatttc ttcttcctct
     1441 gcatccttca tgaagctgtc atcgtccaga tcaagatcca ggccaatgtc atccagagcg
     1501 ggatctgcat tcaacatcgc ttcgtccagt acgggaacta cgtcaatatt acggttctcc
     1561 aggtaactgt aaatctgatc catctgctcg ggagacaggt tgtcccctgt gaaaaagtca
     1621 ttgatctcgc ctgcatccag cgtattgtgc ttggatttgg catgctccac cagcttttcc
     1681 agttttttta aaaaatcgtc cttttccaat gataaacgtc ctttcattta aaggtaaatg
     1741 tggaaactct cacaacttat cattatatac catttgtttc gggatttcaa catctatcga
     1801 caagaatcct tattttctcc cttaaacagc cgatctgagc ctgggttctg gctccgccat
     1861 actctccgtc cagaacccag tccaccggag ctgccgactg aaaacagatg gaagctgtct
     1921 taaaacggta tacatattca ttcggttctt cctttaaaaa cagatgggaa atgatattgc
     1981 tcagatctcc gggagttttt ggcatacgaa tcaaaagcac ctcaaaggca ccatcattta
     2041 aggcaacagt ctggtttacc agatttttaa agccgcccac gctccttgta ttcgtcacca
     2101 tgccgaacac gaaatcctcc tctaattccc cctcttccca ctgcactaac atatggtatg
     2161 gcttaatgga agaaatactt tttaaggcct ccagcatata ggccggatgg cccagcagat
     2221 tctttcgatc ctgggaggta gcataggcca cctctgtaaa agcgccgaaa ccggcaatat
     2281 atacaaaatt ccgatccccg cagaagcttc ccacatccac tgcaaaaggc gccccgttta
     2341 caacattaag agcagcccgc tccatatcct tcgggatctg aaggctgact gcaaaatcat
     2401 tggtagaacc cgcaggaata taccccaatg gcggctgatc cttaagagcc agcatcccgc
     2461 agatggtttc atttaaggtt ccgtctcctc cgctgcacag gataaggtcc atgccctctc
     2521 ccagctcttt tacgatacgg gaggcatctc cctggctctg ggttacatga acatggatat
     2581 catacccatc ctttgtaaaa atatccagga tccgcaaaag gcttccccgg atctgttcct
     2641 ttccggagcg cggattaaat acaaagagca tttgcttcac tgtccatcct cctttttaaa
     2701 caggatctct ctgtaagcgg aaaatgcatt gggaagggga tactgttttt ctatctcctc
     2761 tctggatgcc ataaaaacac cgtccggcag ttccctccag ggttctttaa ggacagcatg
     2821 gtatccgctc atacgccact caatatgaga aaacacatga cgggcatctg gaagaggttt
     2881 caaacatacc agatcatcct cagacactgc cagatacgcc tccgcctctt ttggcgacag
     2941 ctttccctct gcattgggaa gctcataaag agaggccaaa agcccctttg gaggcctttt
     3001 gcggatggcg gccttctcct gatattcaat aataaatacc gtcctttctt ccaccttccg
     3061 ctccttgggc ggggacttcc acgggatttt cttccaaagg ccattttggg ccgtaaggca
     3121 tacggattcc attgggcatt ccctgcattt gggttctccg ccggggatgc acaccagcgc
     3181 tccggtctca atcaatgcct gattatagct tcccgcgcag tccttcggca tcacttcctt
     3241 taaaagagcc tccatggatg tttttacaga ggcttttcgg atgtccgagg aatctgccag
     3301 aatacgggaa acgacccgaa gcacatttcc atccacagcc ggaacgggga taccgtaggc
     3361 aatcgaagca atggctcccg ccgtataact gccgattccg ggaaggctca aaagcgcttc
     3421 ataggaagca ggaagcgctc cgttaaagcg ctccatcacc tgaatggcag ctgcttttag
     3481 atttctggcc cggctgtaat agcccagccc ttcccacagt ttcatcaggc ggtcatcctc
     3541 tgcctgcgcc agatcacgaa tttcgggaaa cgcctccata aaccgctcga aatacggctt
     3601 tactgcctct acccgggtct gctgcagcat aatttccgat atccataccc tgtagggctt
     3661 tggatcttct ctccagggca gctttctggc tctggaggcg taccaggcaa ggagcggctt
     3721 ttctaccgcc cgaagccgct ccctctgctc aagagaccga tccttctctt ccagaatctg
     3781 gagcctgtca taaaaatgca gccttccgtc acacatagcc gtatatcccc cgcacagata
     3841 cctctcctga aataactgct gatacagaat catcctctct cttcaccaaa agacagcctt
     3901 ctttatcaat tccaagagcc ataccctcat aggaaccgtg agggtctaac acgcgaacct
     3961 gtcgtttccg gtttgcaagc atttcattgt actcatccat aagccctgtc atatcaccat
     4021 attccataaa acggctgtaa tactgctcaa agctcttaag caccgctccg atcaaaggcg
     4081 cccgtttaag ctcatggccc agagacagct ttaaagaggt agcagtgtct gaaatttcag
     4141 gtggaaagct ttctgtattt acattgatac cgatccccac tactacatag cggatggaat
     4201 cctcctctgt gctcatctcc gttaagatgc cgcaaatctt tttcccatcg accacagcat
     4261 catttggcca cttgatctga ggggacagac ccgaaatctg ccggatcccg gccaccactg
     4321 ccatcccggc caccagcgtc agcatagagg cttttgacgg agacaactca gggcgaagta
     4381 taaaactcat ccagactccc acaccgcgag gagaggccca ggcccgtcct cttcttcctt
     4441 ttcctgcgct ctggctgtct gctgcaacaa gagttccatg gggtgcaccc tcctccgcca
     4501 gttttctggc ctgacggtta gtggaatctg tctcataaaa gcagttcacc tgagacccca
     4561 gccacacggt ggaaagaact gttttaagtt ccggggctgt gatcacatcc ggagaagcca
     4621 cgagacggta tcctctgttt ctcaccgcct caatggcata gccctcctcc tccagctggc
     4681 gtatcgcttt ccatacagct gttctggaaa cccccaggcc ctcgcacagc tgctggcccg
     4741 acagaaaatc tggtgtttcc ttcagcattt ttaaaatttc tgttttcatt cttctctccc
     4801 atcatctatc cgtgaaacca tagacttacg gcgcttaaca cgccaagaag cccaagacct
     4861 gctccaagaa gcagctggaa gattcccagc ctgaaccgct tcttatctct ggcacaggtc
     4921 ttaagctcaa atatgccttt gatcccattt aacagcgccg ccagcaaaaa taccagggga
     4981 aacagcatcc tgttcccttc ggggcttata aaggacagca cggcgaatat gacaatggcg
     5041 atcccaagga tcaagtgcgc catgtcgaga aataccccgg cacggcgctc atttcttcct
     5101 ctctggccct gcatatctgc attctccatt tctccggatg cagaccttac agaacccaga
     5161 gcctactccc caagggttgc aagcattacg gccttaatgg tgtgcatccg gttctcagcc
     5221 tcgtcaaata caactgactg gctggactca aatacttcat ctgttacttc cagctctgac
     5281 acatggaagc gctcgcccat ctcctttcca atctttgtct taagatcgtg gaaggaaggc
     5341 aggcagtgaa ggaaaatggc ctgggaacct gccccgtcca tcacttcctt tgttacctta
     5401 taggggctta attcccggat tctctcttcc catacctcgt ctggttctcc catggatacc
     5461 caaacgtctg tatcgatcac atctgcgccc tttacagcct ctgccacatc ctctgtcagg
     5521 gtgatcgtac cgccgctttc tgccgcaaag gcctcacatt cttttaccag tgccggatcc
     5581 gggaaatact ttttcggtgc acaggcagta aaatgcattc ccatcttggc acaggcgatc
     5641 ataagggaat tacccatatt atagcgcgca tcgcccatat acacaagctt aactccctta
     5701 agccggccca gatgttctct gatagtgagc agatccgcca gcatctgggt agggtgatcc
     5761 tcatttgtaa gtccgttcca tacaggaact cctgcataat gagccagctc ctctacaatc
     5821 tcctgtccaa agcctctgta ctcaataccg tcgtacatcc ggcccagaac tctggctgta
     5881 tctgcaatac tctccttttt tccgatctga gagccggatg gatccagata agtggaaccc
     5941 attcccagat catgggctgc cacctcaaag gcacagcggg ttctggtact ggtcttttca
     6001 aagatcagcg ccacattttt tccctttaag gtatccgtaa gaatcccttt tttcttcttg
     6061 tcctttaatt ctgccgccag atccagcaga tacaggattt cctcctgtgt ataatctttc
     6121 aatgtcaaaa aatttcttcc ctttaaattc atagcatcct ccgtgatctt ttccagactt
     6181 atttggccag aaatttatat ttatgcatct tttatgcata atataccacc atgagaatgc
     6241 gatgtcaaga atttttatct ccagctgcgc cttcttaaaa ccgttaaaag ctgatccggc
     6301 gtatagatcc gaaaccggct gacccgatat ttctttgcag cttcttccag aagggaaaaa
     6361 taaagctgcc tgtaatccca tttttccttt aatcccagct ttgcggcaag acgcggaaac
     6421 agctcctcca tataaatccg cagggaaccg ccctctttat tttctgtatg ttccgccccc
     6481 atcccgcacc agtccataag ctccaaaact tcagccatca gacggttaaa ataatactcc
     6541 tcgctttcag gagcatccag ataatagatc cttccctcca gcccatagag catacgcatg
     6601 gcatcaaaat atccaaggag catattcctt ctggcctttt tccggtcaaa ttccagaatg
     6661 cctcccagtt cctgccttgg cgctacatga taaaggcgaa cgccgtcagg cacctgaaga
     6721 cggcgttccg tatctacccc atagccgtag atccgaagga cgataatatc ctcatacccc
     6781 cggtcgatca gcacatctac aggcacatta ttgaccctgg caccgtccat gtaatacttt
     6841 ccgcccagct tttccggttt aaagccgatc agatacgcac ttgccataag catgtcgcag
     6901 atctgtcccg gaggtacttc ctttatatca accacaagag gctgatgttc cgtcagggaa
     6961 taggtaactg tatataaatc cctgtcagag gtgcgtattt tcgcctcatc aatcgccccg
     7021 cggatcagct cccgcaaagg tgtgatatca aagccgcctt cttttaacag gcgttttact
     7081 ccgtttagaa gatccgaaag tcttacggct gtaccgtttt ttaagttcca ctccctcaaa
     7141 gcttccattc ttttgtcatc tacatccata acctgggaat agcggatatt ttcccagatt
     7201 ttctccgccg tttcgaagtc atccatacag atcagggcgc cgtttaaagc tcccacagag
     7261 gttcctgctg ccccctgtat ctgtatgccc gcttctttta aagccttcca ggctccgatc
     7321 tgatacgctc ctctggctcc tccgccctcc agtacaatac cgtacctgcg gcctttatcc
     7381 aactgcagct tcaccttctc ctccttgcgg ccgctccgat gatccacagt gaaaaaacag
     7441 acacaagaag aaacacaaaa agctgctcct ctgccgtccg ccccataaga gcccctatcc
     7501 aggctcctaa agcccctgca caaaaccaga gcgaaataag gctgcggaaa aagagctcca
     7561 cagccataaa gaataaatat gcaaaaaacc agcctgcaat ccctgtcatg gattttctcc
     7621 tttcaggggc ctcccacgct caggccacgc cgcagactgt ctctgtcaga tatttctgcg
     7681 ctgtctggct gcctcaaaca tcagcacaga agctgctacc gccgcattta aggactcaac
     7741 ctttcctgcc atgggaatct ggatacagca gtctgccgcc tctgccgctg cctctgtaag
     7801 accgtttgcc tcatttccaa tgagaaagcc cacatttcca gtataatcct ggcggtcata
     7861 attattcatt cccttcaaat gtgccgcaaa aagacgcacg ccctctctgc caagcctttt
     7921 gcagacagct gtaaaatcct ccgtataaag gaagggcatt cgcagcacgg aacccatggt
     7981 agagcggatc accttggggc tgtagatatc tacggtgtca cggctcatga tcacaccggt
     8041 tactcctgcc ccctctccgg cccgaaggat cgtccccaga ttgccgggat cctgaatatt
     8101 ctcaaggagc atgagaagaa ggggagttcc ggaagatgct ggctttaaaa gctcctccag
     8161 ggtgtatttc ttctgccttg ccaccgccag cacaccctga ggcgtctttg tatccgccat
     8221 ggccttcata acttcttcgg tgacaatttc gtaggaatag gagtgaagcc actccctgtt
     8281 ttccttctgg ctctcaaagg acggcgtaac
//
LOCUS       DBMDUH010000121         8097 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_3979_length_8097_cov_9.396948, whole genome shotgun sequence.
ACCESSION   DBMDUH010000121 DBMDUH010000000
VERSION     DBMDUH010000121.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 8097)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8097)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8097
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3979_length_8097_cov_9.396948"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /gene="jag"
                     /locus_tag="ACZPCG_10035"
     CDS             <1..777
                     /gene="jag"
                     /locus_tag="ACZPCG_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HIR1285430.1"
                     /translation="SAKTVDEAVTKALIELKTTSDKLEYEVVEEGSKGFLGLGAKPAV
                     IRAKKKETVADGAIEFLTQIFDAMGMDVSIKSEYNEGEQELSLELEGGDMGILIGKRG
                     QTLDSLQYLVSLIVNKETDGYLRVKLDTENYRERRKETLETLAKNIAYKVKRTKRPVS
                     LEPMNPYERRIIHAAIQNDKYVITRSEGEEPFRHVVIALKKETGAGERRGRYDRSRGG
                     RYERTGGYRGNTRRNDSYQAASLSEHNAETAGGSGQETEE"
     gene            865..2244
                     /gene="mnmE"
                     /locus_tag="ACZPCG_10040"
     CDS             865..2244
                     /gene="mnmE"
                     /locus_tag="ACZPCG_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HIR1285431.1"
                     /translation="MKTETIAAIATGMSNSGIGIVRISGDQAFEVIDRIYRNKKGQAG
                     TLWDAKSHTAHYGHIYDGDERIDEVLVLIMRGPNSYTAEDTVEIDCHGGIRMVQRILE
                     TVLHNGARTAQPGEFTKRAFLNGRLDLAQAEAVAELIHASNDYALKSSVSQLGGSVSG
                     KIRALRETILYETAFIESALDDPEHISLEGYGDRLLKVLEPVTEEIEKLIASADDGRV
                     MTEGVRTVILGKPNAGKSSLMNVLLGEERAIVTDIAGTTRDTLEEHINLQGISLHVVD
                     TAGIRDTEDVVEKIGVNRAMKAAKGADLIIYVADGSRPLDESDREIMELIHDKKAIVL
                     LNKTDLELTVDKEALEEQCGHRVIPISAKEEHGIGDLEKEIQSMFYHGEIAFNDQVYI
                     TNARHKEALEAALRSLRMVRSSVLDGMPEDFYSIDLADAYEQLGLILGEAVDDDVVNE
                     IFSKFCMGK"
     gene            2268..4139
                     /gene="mnmG"
                     /locus_tag="ACZPCG_10045"
     CDS             2268..4139
                     /gene="mnmG"
                     /locus_tag="ACZPCG_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HIR1285432.1"
                     /translation="MPYLEESYDIVVVGAGHAGCEAALACARLGLETIMFTVSVDSIA
                     LMPCNPNIGGSSKGHLVRELDALGGEMGKNIDKTFIQSKMLNESKGPAVHSLRAQADK
                     QAYTQAMRQTLEQTEHLTIRQAEVSELIVKEGAVRGVKTFSGAVYHAKAVVLCTGTYL
                     RARCIYGDVSNPTGPNGLQAANHLTQSLIDNGIEMFRFKTGTPARVDKRSIDFAKMEE
                     QFGDERIVPFSFSTDPDSIQKEQVSCWLTYTNGETHQIIRDNLDRSPLFSGAIEGTGP
                     RYCPSIEDKVVRFPDKERHQVFVEPEGLYTNEMYLGGMSSSLPEDVQYAMYRTVPGLE
                     NVTIVRNAYAIEYDCINALQLKSTLEFKRIRGLFAGGQFNGSSGYEEAAVQGFMAGVN
                     ASMEVLGREQVVLDRSQAYIGVLIDDLVTKENHEPYRMMTSRAEYRLLLRQDNADLRL
                     REIGHKIGLVSDEEYEKLLSKRQAIEEEIRRLENTAIGGTPAVQAVLEQYGSTPLKSG
                     STLAELVRRPELDYEKIREIDKKRPDLPFDVREQVNIEIKYEGYIKRQRQQVAQFKKL
                     EGRHLPENFDYSQVKSLRREAVQKLNQIQPSTVGQASRISGVSPADISVLLVYLGRE"
     gene            4177..4941
                     /gene="rsmG"
                     /locus_tag="ACZPCG_10050"
     CDS             4177..4941
                     /gene="rsmG"
                     /locus_tag="ACZPCG_10050"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009263543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HIR1285433.1"
                     /translation="MTESFAEKMKAGTRELNITLSQRQLEQFFKYYEMLVETNKVMNL
                     TAITEEGDVVAKHFCDSLCLVKAYHELLDRESEGEAKDMKTVSVIDVGTGAGFPGIPL
                     KIAFPQIKLTLLDSLNKRVKFLEQVCRELELENVQCIHGRAEDAGRMKEHREQYDLAV
                     SRAVANLASLSEYCIPFVKKGGFFIPYKSGEIEEELNQGKKAAAILGGEINEVVRFSL
                     PEGDGARSLIRIRKVKPVSSRYPRKAGMPGKEPLGK"
     gene            5089..6171
                     /locus_tag="ACZPCG_10055"
     CDS             5089..6171
                     /locus_tag="ACZPCG_10055"
                     /inference="COORDINATES: protein motif:HMM:NF012742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR1285434.1"
                     /translation="MNIKEIARVAGVSPATISRVLNGSGYVKEETKKKVMAAVEKNNY
                     TPSLIARSLSGRDSFSIGVILPSASQRVYSGILEGICRLAQPFGYNIVLMNTGGDIKK
                     QHEFLEAAENQRMKGLIVAPAASDDRGTRDKLLHLEEQGMPVILTEGNIKGAPFDCVF
                     GDNWRGAYEGAESLMDAGHKKIAVIREKGNFREDRERFRGFMQAMEDHGLEVSDEYVI
                     YNGESGAETADAIARLLEENTPPTALFALSGAGTCSCMKCLERKGLTAGEDLGFMGFD
                     DQPVLYELGYRLSVVERDAVEQGREAMRLLQERFDEKEQERRIGKRVMVPCRLILRGS
                     GNMKTVTESARKKKLAGKKEKESPER"
     gene            complement(6168..6998)
                     /locus_tag="ACZPCG_10060"
     CDS             complement(6168..6998)
                     /locus_tag="ACZPCG_10060"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015573241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HIR1285435.1"
                     /translation="MLRIGCHLSSAKGYCSMAKDAAKIHANTFQFFSRNPRGGNAKEI
                     NPEDVALFLKKVQEQNLIPILCHAPYTLNACSADPHLRDFARKTMADDLARMEYTPGN
                     LYNFHPGSHVKQGVDVGISYIADMLNAILKPEQTTTVLLETMAGKGSEIGRSFEELRQ
                     ILDRIDLSDHMGICMDTCHVWDAGYDIANHLDDVLAEFDHIIGLNRLKAIHLNDSMNP
                     LGSHKDRHARLGEGQIGLPSLKAIINHPALRHLPFYLETPNDLDGYAREIAMMREAWS
                     "
     gene            complement(7097..8056)
                     /locus_tag="ACZPCG_10065"
     CDS             complement(7097..8056)
                     /locus_tag="ACZPCG_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR1285436.1"
                     /translation="MKTRNKLLTLLILAAGDAAATACINKAIKLSAISRHILENESSA
                     SLCYHWRFGNIHYTKEGSGKPILLIHDLSPASSGYEWKEFARRLAKDRTVYTIDLLGF
                     GRSEKPNLTYTNYLYVQLISDFIKSEIGHRTDVAATGDSSSIAVMACSNSPELFDRLL
                     LINPESLLSSSLVPGKNARLYKLILDLPIAGTLIYNIACSRKNLTQDFMSRCFCNPYT
                     VNSRLVDAYYEAAHLGASPKSIYASVKCNYTKCNITTALRRIDNSICLFGGAELDCMS
                     ETMEDYQNYNPAIETVPVPKAKHLPHLESPARFYELFRPYLEN"
BASE COUNT         2297 a         1579 c         2355 g         1866 t
ORIGIN      
        1 tctgcaaaaa cagtggatga agcggttacg aaagctctga tcgaacttaa gacaaccagc
       61 gataaactgg agtatgaggt tgtggaggaa ggaagcaaag gctttctggg gctgggcgca
      121 aagccggctg tgatccgcgc aaagaagaag gaaacagtgg cggacggggc cattgagttt
      181 ctgacccaga tatttgacgc tatgggtatg gacgtatcca ttaagtcgga atataatgaa
      241 ggggagcagg agctgtcctt ggaattagag ggcggagata tgggaatcct gatcggcaag
      301 agaggtcaga ccctggattc tcttcagtat cttgtaagcc ttattgtaaa caaggaaaca
      361 gacggttatc tgagagtcaa actggatacg gaaaattacc gtgagcgcag aaaagagact
      421 ctggagaccc tggcaaagaa cattgcctac aaggttaaga gaaccaaacg tcccgtttct
      481 ctggagccca tgaatcccta tgagagaagg atcatccatg cggcgatcca gaatgataag
      541 tatgtgatta cccgcagtga gggggaagaa cctttccgcc atgtggtaat cgctcttaag
      601 aaggaaacag gcgccggtga gcgcagggga cgctatgacc gcagccgcgg cgggcgctat
      661 gagcgtaccg gcggctaccg gggaaatacc cgcaggaatg actcctatca ggcggcttcc
      721 ctgtcagagc ataatgctga gacagccggc ggcagcggac aggaaacaga ggagtaatcc
      781 ggtaaatgaa catggggagg ctgttgcaga gtgatttgcg gcggcctctt tttgcgctga
      841 aagccagaaa ggcgaggagt gaaaatgaaa acagaaacca ttgctgccat tgccacaggc
      901 atgagcaatt cgggaatcgg cattgtaagg atcagcggag atcaggcctt tgaggtcatt
      961 gaccgaatct atcggaataa aaaggggcag gcaggaacat tatgggacgc aaagtcccac
     1021 acagcccatt atggacatat ttatgatggt gatgaaagga tcgatgaggt tctggtgctt
     1081 attatgcggg ggccaaacag ctatacagca gaggataccg ttgagattga ttgtcatggc
     1141 ggcattcgca tggttcagcg gattctggaa acggtccttc ataacggggc cagaaccgcc
     1201 cagccgggag agtttaccaa gcgcgccttt ttaaacggaa ggctggatct ggcccaggca
     1261 gaggcagtgg cagaactgat ccatgcatcc aatgactatg ccttaaagag ctctgtgagc
     1321 cagctgggag gatcggtatc gggaaagata cgggctctca gggaaaccat tctttatgag
     1381 actgccttta ttgagtcggc tctggatgat ccggagcaca tttcactgga ggggtacggg
     1441 gacaggcttt taaaggtgct ggagccggtg acagaagaga ttgaaaagct gatcgcctcg
     1501 gcagatgacg gaagagtgat gacagagggc gttcggacgg tgatccttgg aaaacctaac
     1561 gcaggaaaat cttccctgat gaatgtgctt ttaggggaag agagggctat tgtaacggat
     1621 attgcaggta ctaccagaga taccttagag gagcatatta accttcaggg gatcagcctg
     1681 catgtagtgg atacggcggg aatccgggac acggaggatg tagtggaaaa gatcggcgta
     1741 aaccgtgcca tgaaggcagc aaaaggagcg gatctgatca tttatgtggc agacggctcc
     1801 cgcccgctgg atgagagcga ccgggagatc atggagctga tccatgataa gaaggccatt
     1861 gtgcttttaa ataagacgga tctggagctt actgtagata aggaggcttt agaggagcag
     1921 tgcggccacc gtgtaatccc tatttccgcc aaggaggagc atgggatcgg agatctggaa
     1981 aaggagatcc agtccatgtt ttatcatgga gagattgcct ttaatgacca ggtatatatt
     2041 acaaatgccc gtcataagga ggctttggaa gctgccttaa ggagccttcg gatggtgcgt
     2101 tcaagtgtgc ttgatgggat gccggaggat ttttattcca tagatcttgc agacgcctat
     2161 gaacagctgg gactgatctt aggagaagca gtggatgatg atgtggtaaa tgagatcttc
     2221 tcaaagttct gtatgggaaa atgagaggac aggaaggaag gaaaaagatg ccgtatttgg
     2281 aagaaagtta tgatatcgtg gtggtgggag caggccatgc gggctgcgag gcggcgttag
     2341 cctgcgcgcg gcttgggctt gaaaccatca tgtttacggt cagtgtggac agcattgccc
     2401 tgatgccctg caatcccaat ataggaggca gctccaaggg ccatctggtc cgtgaactgg
     2461 atgcgcttgg gggagaaatg ggcaaaaata ttgataagac ctttatccag tccaagatgt
     2521 taaatgaatc caaggggccg gcggttcact ccctcagggc ccaggcggat aagcaggcct
     2581 atactcaggc catgaggcag accctggaac agaccgaaca cctgaccata cgccaggcag
     2641 aggtatcgga gctgattgta aaagagggcg ctgtaagagg cgtaaagacc ttttccggcg
     2701 ctgtatacca cgcaaaggcg gtcgttttgt gtacaggcac ctatctgcgg gccagatgca
     2761 tttacgggga tgtaagcaat cctacagggc ctaatggcct tcaggcggcc aaccacctga
     2821 cacagtctct gattgataat gggatcgaga tgttccggtt taagacggga actccggcca
     2881 gagtggataa gagaagcatt gattttgcaa agatggagga gcagtttgga gatgagcgga
     2941 ttgtgccgtt ttcattttca accgatccgg acagcataca gaaggagcag gtttcctgct
     3001 ggctgaccta taccaatggg gagacccatc agatcatccg ggataatctg gaccgttcgc
     3061 cgctgttttc aggagccatt gagggaacag gccctcgata ttgtccgtct atcgaggaca
     3121 aggtagtgcg ttttcctgat aaggagcgcc atcaggtgtt tgtggagccg gagggacttt
     3181 ataccaatga aatgtatctg ggcggaatga gcagctctct gccggaagac gtgcagtatg
     3241 ccatgtaccg caccgtaccg gggttagaga atgtaacgat cgtgcgcaat gcctatgcga
     3301 tcgagtatga ctgcatcaat gccctgcagt taaagtctac cctggagttt aaacgcatca
     3361 gaggcctgtt tgcgggcggt cagtttaacg gaagctccgg atatgaggag gcagcagttc
     3421 agggctttat ggcgggcgta aacgcgtcta tggaggtgct gggaagagag caggtagtgt
     3481 tagaccggtc acaggcctat atcggcgttc tgattgacga tctggtgacc aaggaaaacc
     3541 atgagccata ccggatgatg acttcaaggg cagagtaccg gcttttgctg cgccaggata
     3601 atgccgattt acggcttcgg gaaatcggtc ataaaatcgg actggtaagc gatgaggagt
     3661 atgaaaagct gctctctaaa cgtcaggcca tcgaagaaga aatacgccgt ttagaaaata
     3721 ctgccatcgg cggaacgccg gcagtccagg cagttctgga gcagtacgga agtacgcctc
     3781 ttaaatcagg aagtacgctg gcagagctgg tgcgccggcc ggagctggat tatgaaaaga
     3841 tcagggaaat tgataaaaaa aggccggatc ttccatttga tgtgcgggag caggtaaata
     3901 ttgaaatcaa gtatgagggc tacataaaga ggcagagaca gcaggtagcc cagtttaaga
     3961 agctggaagg caggcatctg ccggaaaact ttgattacag ccaggtaaag agcctgcgtc
     4021 gggaagctgt tcagaagctg aatcagatac agccgtctac cgtgggacag gcatcacgga
     4081 tttccggggt atctccagcg gatatatcag tgcttttagt gtatctggga agagagtaaa
     4141 agatggtttt tacatataaa agagaggacg ggatcaatga cagaatcatt tgctgagaaa
     4201 atgaaggccg ggacaaggga gctgaatata accctgagcc agaggcagtt ggaacagttt
     4261 tttaaatatt atgaaatgct ggtggagaca aataaagtga tgaatctgac agccattaca
     4321 gaggaagggg acgtggtggc aaagcatttc tgtgacagtc tttgcctggt aaaggcgtat
     4381 catgagcttc tggatcggga gtctgaaggt gaagctaaag atatgaaaac ggtttctgtg
     4441 attgatgtgg gaaccggagc cgggttccct ggaatcccgc ttaagattgc atttcctcag
     4501 attaaactca cgctgctgga ttcgctgaat aaaagagtaa aatttttaga gcaggtatgc
     4561 agagagctgg aactggagaa tgtacagtgc atccatggaa gggcagagga tgcaggacgg
     4621 atgaaagagc acagggagca gtatgatctg gcagtgtcca gagcagtagc aaatctggca
     4681 tcgctgtcag aatattgtat tccttttgta aaaaaagggg gattctttat tccttataaa
     4741 tcaggagaaa ttgaggaaga gctgaatcag ggaaagaaag cagcagcaat tctgggagga
     4801 gaaataaacg aagtggtgcg cttttcttta ccggaaggag atggagccag aagtctgatc
     4861 cgtattcgta aagtaaagcc ggtatcttcc agatatccaa gaaaagcggg aatgccgggg
     4921 aaagaacctc tgggaaaata ggcgcgaagg cagagcatat gtgggtgaga tcagtgccgc
     4981 agaagagatg gaaaacggtt acacgtactt tatgacgcat gatgcaaaaa caggggatac
     5041 actatgaaaa cgattacaga aaatacagta caggcaggac aaggtcgcat gaatattaaa
     5101 gaaattgccc gggtggcagg agtatctccg gctaccatat cccgtgtact aaatggttcg
     5161 ggatatgtga aagaagagac aaagaaaaag gtaatggcgg cagttgagaa aaataactat
     5221 acgcccagtc tgattgcaag gagtctgagc ggaagagaca gcttcagtat tggagtgatt
     5281 ctcccttccg caagtcagag agtttacagt gggattctgg aagggatctg ccgtctggct
     5341 cagcctttcg gctacaatat tgttcttatg aacacaggag gggatataaa gaagcagcat
     5401 gagtttctgg aggctgctga aaaccagaga atgaaagggc ttatcgttgc tcccgcagct
     5461 tctgatgaca gggggacgag agataaactt ctccatctgg aagagcaggg aatgccagtg
     5521 atcttgacag aagggaatat aaaaggagca ccgtttgact gtgtgtttgg agataactgg
     5581 aggggggctt atgaaggcgc tgagtccctt atggatgcag gccataaaaa gattgcagtc
     5641 attcgtgaaa aaggcaattt cagagaggac agggagcgtt ttcggggctt tatgcaggca
     5701 atggaggatc atggcctgga ggtttccgat gaatatgtaa tatacaacgg agaaagcgga
     5761 gcagaaacag cagatgcgat tgcgcggctt ttagaagaaa atacaccgcc tacggctctg
     5821 tttgcactga gcggggcagg aacatgcagc tgcatgaaat gtctggaaag gaaggggctg
     5881 acggcaggag aagatctggg ctttatgggc tttgatgatc agccggtttt atatgagctg
     5941 ggatatcgtc tttcggttgt ggagcgggat gcagtggaac agggaaggga agcgatgaga
     6001 ctgcttcagg aacgatttga tgaaaaagaa caggaacgca gaatcggaaa gcgtgtaatg
     6061 gttccatgca ggctgattct gcgtggatcc gggaatatga aaacggttac agaaagtgcc
     6121 agaaaaaaga aattggcagg aaaaaaggaa aaagaaagcc cggagcgtta actccaggct
     6181 tcgcgcatca tggcaatctc acgggcatag ccatccagat cattcggtgt ttccaaatag
     6241 aagggaagat ggcgtaaggc gggatgattg ataatggctt ttaaggacgg aaggccaatc
     6301 tggccttcgc ccaaccgggc atggcggtct ttgtgagaac caaggggatt catgctgtca
     6361 ttgagatgga tggctttcag acgatttaaa ccaatgatgt gatcaaattc tgcgagaaca
     6421 tcatccagat ggtttgcgat atcatagccg gcatcccata catgacaggt atccatacag
     6481 atccccatat ggtcggacag gtctatccgg tcaagaatct ggcgaagttc ttcaaagctg
     6541 cggccgattt cgcttccttt tccagccatg gtttctagga ggacagtagt ggtttgttcc
     6601 ggttttaata tggcatttaa catatctgcg atgtaggaaa ttcccacatc aacaccctgt
     6661 ttcacatgac ttcccggatg gaagttataa agatttccgg gagtatattc catccgtgcc
     6721 agatcatctg ccatagtttt tctggcaaaa tccctgagat gaggatcggc agagcaggca
     6781 ttcagagtat agggggcatg acagagaatg ggaatcagat tctgttcctg caccttttta
     6841 agaaatagag ctacatcctc aggattgatt tctttggcat ttcctccgcg ggggttgcgt
     6901 gagaaaaact gaaaggtgtt tgcatgtatt ttggcagcat cctttgccat ggaacagtag
     6961 cctttggcag aggaaagatg acagccgatt cgtaacatat aggagcctcc tttaacattg
     7021 tttttatgta aaattgatcg gcagtttctg cagaaatgtt tcacgtgaaa catttatagt
     7081 agaaactgcc ggtattttaa ttttccagat aagggcggaa taattcataa aatcgagcag
     7141 gggattccag atggggcaaa tgtttcgcct taggaactgg cactgtttcg attgcaggat
     7201 tgtaattctg ataatcttcc atggtttcag acatacaatc caactctgct ccgccaaaaa
     7261 gacagatgct gttatcaatc cggcgcagag cggttgtaat attgcatttc gtgtagttac
     7321 attttacact ggcataaatg gattttggag aagctcccag atgggctgct tcatagtaag
     7381 catcaaccag ccgggaatta acagtgtagg gattacagaa gcagcggctc ataaaatcct
     7441 gagtaagatt ttttcggctg caggctatat tgtaaatcag agttcctgca atgggaagat
     7501 ccaaaatcaa cttataaagc cgggcatttt ttcccggaac cagactgctg gagagcagag
     7561 attcgggatt gatcagaaga agccggtcaa agagctccgg actattgctg caggccatga
     7621 cagcaatcga agaggagtct ccagttgccg ccacatcggt gcggtgaccg atttctgatt
     7681 taataaaatc tgaaatcagc tggacgtaaa gatagtttgt gtaggtgaga ttcggctttt
     7741 ctgaacggcc aaatcccaac aggtcaatgg tatagacggt tcgatccttt gcaagacgtc
     7801 gggcaaattc tttccattca taaccgctgg aagcggggga gagatcatgg atcagaagaa
     7861 tcggtttacc actgccttct tttgtatagt gaatgtttcc aaagcgccag tgatagcata
     7921 gagaggcgct gctttcattt tcaagaatgt gtctggaaat cgcagacagt ttaatggcct
     7981 tgttgataca ggcagtggcg gcagcatcac ctgcagccag tatgagcaga gtcaataatt
     8041 tatttcttgt tttcattcgt tttatctcct gaaaaagtaa tgataggtgt cgtgtgc
//
LOCUS       DBMDUH010000122         8029 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_4015_length_8029_cov_7.745207, whole genome shotgun sequence.
ACCESSION   DBMDUH010000122 DBMDUH010000000
VERSION     DBMDUH010000122.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 8029)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8029)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8029
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4015_length_8029_cov_7.745207"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(99..230)
                     /locus_tag="ACZPCG_10070"
     CDS             complement(99..230)
                     /locus_tag="ACZPCG_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285437.1"
                     /translation="MITLPVAVEQETVWLNRNQMAELFGRDIKTIGKHINNALNEEL"
     gene            complement(350..1741)
                     /gene="gltA"
                     /locus_tag="ACZPCG_10075"
     CDS             complement(350..1741)
                     /gene="gltA"
                     /locus_tag="ACZPCG_10075"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006774570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HIR1285438.1"
                     /translation="MDVLKRVPIREQEPKVRAANFEEVCLGYTQEEAQEEASRCLNCK
                     NAQCVKGCPVSINIPKFISEVKEGKFADAAATIAESSALPAVCGRVCPQESQCEGKCI
                     RGIKGEPVSIGKLERFVADWSRENGVVPAKPEKTNGIKVAVIGSGPSGLTCAGDLAKM
                     GYEVTIFEALHEPGGVLTYGIPEFRLPKTRVVRPEIENVKKLGVKIETDVIIGKSVTI
                     DELMDEEGFKAVFIGSGAGLPKFMGIPGENANGVFSANEYLTRSNLMKAFRDDYDTPI
                     FLGKKVAVVGGGNVAMDAARTALRLGAEVHVVYRRSEAELPARVEEVHHAKEEGVQFD
                     LLTNPVEILTDENDWVTGMVVRKMELGEPDASGRRRPVEVEGSDYTIEVDTVIMSLGT
                     SPNPLISSTTEGLQTNKWKCIIADESNGKTTKEGVYAGGDAVTGAATVILAMEAGKAG
                     AKGIDEYLKGKEA"
     gene            complement(1741..2631)
                     /locus_tag="ACZPCG_10080"
     CDS             complement(1741..2631)
                     /locus_tag="ACZPCG_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HIR1285439.1"
                     /translation="MYKIVKAECLADKIYLMDVEAPRVARACQPGEFVIVKIDEVGER
                     IPLTICDFDREKGLVTIVFQTVGASTQRMAELKAGDYFQDFVGPLGQPSEFVKEDIEE
                     VKKRKYLFVAGGVGSAPVYPQVKWMHEHGIEADVIEGSKTKDLLILEDELKAVAGNLY
                     VTTDDGSYERKGMVTDVIKDLVENQGKKYDVCVAIGPMIMMKFVCKLTKELGIPTIVS
                     LNPIMVDGTGMCGACRVTVGGEVKFACVDGPEFDGHLVDFDQAMKRQQMYKTEEGRAI
                     LKAQEGATHHGGCGNCGGDE"
     gene            complement(2719..3630)
                     /locus_tag="ACZPCG_10085"
     CDS             complement(2719..3630)
                     /locus_tag="ACZPCG_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HIR1285440.1"
                     /translation="MKNDGTVFTLENEELLITVDRHGAELTRIYDKKAGRDLLWGADP
                     AVWNRHAPVLFPFVGKSFDGKYTHEGKEHGITPHGFARDMEFEPLLCDMDECWFRLKD
                     TPDTYGVYPFHFELEIGHRLTGRTIEVMWRVKNPDSGELLFMIGGHPAFQVPEGKTIY
                     DYTLVFNKEGKEAGNHQEELHYLAPNDQGYQVEEKTGSLKLKDGQVPVTKGFFDQILT
                     YMFDGAQISSVGLLVDETPYVTIDCGDFPYLGVWTIEATHPFVCLEPWYGLCAPDGYK
                     GELKDRPGIQKLEAWGTWEKSYSIRIE"
     gene            complement(3747..4244)
                     /locus_tag="ACZPCG_10090"
     CDS             complement(3747..4244)
                     /locus_tag="ACZPCG_10090"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HIR1285441.1"
                     /translation="MNLIVSADKHWSIGKNGRPLVNIPAERQLLLKETAGKVVVMGRK
                     TLETLPGGQPLGGRTNVVLSADPSFKARGAVVCRSMEETLAYLEEFDPKDIYILGGES
                     IYRQFLPYCDTIHLTAIDYVYDADLRFPVDLDQAPDWKLAEEGEEQTYFDLCYTFCRY
                     VRTGM"
     gene            complement(4248..4763)
                     /locus_tag="ACZPCG_10095"
     CDS             complement(4248..4763)
                     /locus_tag="ACZPCG_10095"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006776461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HIR1285442.1"
                     /translation="MNLIAAADENWAIGKDGGLLAHLPGDMKFFRETTKEKVVVMGRK
                     TLESFPGKKPLKNRVNIVLTRNTDYAPEGVTLCGSVEEALELLKQYDSEDVFIIGGGT
                     VYRAFLPYCKKAYITHIFHTFPADTVFPDLDREPGWKQTKVLGTGEDNGIRYEFRLYE
                     QQPVSDGKGGI"
     gene            complement(5094..6488)
                     /locus_tag="ACZPCG_10100"
     CDS             complement(5094..6488)
                     /locus_tag="ACZPCG_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HIR1285443.1"
                     /translation="MVTLIQTVYQFLWGDLITLPLPGGAGISFSLLVLLLIPTGIYFT
                     LRTRFLSLRLFKDMLRVTLERKDGETTALSACQTLVVSTATRVGMGNLVGVVAAISAG
                     GAGAVFWMWVTALLGSTTAFIEATLAQLYKEEDPLYGGYRGGPAYYMHKFFQKNKKQT
                     RWFPLSVLFAISGLICWCGISQVISNSVASSFENAFNIPPLYSTIVLVILGAVIVLRK
                     NATVKALDAIVPVMAGLYLLITVGVILLNIGQIPAVLERIFSEAFGLRQAVAGGIGAV
                     IMNGVKRGLFSNEAGSGSAPCAAATAKTDHPAKVGLVQALGVFIDTIVICSCTAFLML
                     LAPDEITGSLAGMDLLQAAMGYHLGKFGVIFIAVILWLFSFSTFLGILYYARSNVSYL
                     FGDSWAAQTAYKVLALVMLFIGGLAAYTIVWDLGDVGVGLMTIFNLIALYPMAGEALA
                     SLKDYEEKRAKGVL"
     gene            6635..7558
                     /locus_tag="ACZPCG_10105"
     CDS             6635..7558
                     /locus_tag="ACZPCG_10105"
                     /inference="COORDINATES: protein motif:HMM:NF015431.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1285444.1"
                     /translation="MNIDTKNMETFIQVAELCSFTKAAKKLGYSQSTVSFQIRQLEEM
                     LNVRLLERINHTVALTEAGRKVLNYAHRLEQLTVDMEKELRPQPSVSGHVRMALADSL
                     ACWILREHYENFRAAYPGITLKMATASTEELFRMLNQNETDLVYTLDHHVYNSDYIIG
                     AEERIQAHFVAAPGFLPFSSLTVPVRELITFPFLLTEKGMSYRRLMDEHLARFSLEVR
                     PVLEFGSADLLCTLVTQGAGVSFLPDYVTEAALKSGRLVRLSVPEFKPELWRQLLYHK
                     DKWVSPAMQTVIEYFSGLSPAHTDASDHMLL"
     gene            7581..>8029
                     /locus_tag="ACZPCG_10110"
     CDS             7581..>8029
                     /locus_tag="ACZPCG_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HIR1285445.1"
                     /translation="MNQSESSTPGDRFAQILQFQTPAALAWIRGNTPYAPGLSGMVRF
                     YDTPYGGVLIEGEFFRLPNKGISGSTDFYALHIHENGDCSAGFTRTGGHYNPTGTSHP
                     WHSGDLLPVMGNSGYGWLSFYDKRFTVKEIMGRSVVLHSGLDDFTSQP"
BASE COUNT         1924 a         2202 c         1783 g         2120 t
ORIGIN      
        1 cccgtacggg tatctacata ggacaatagg gattcttcct gatgtccggt gcaagacatg
       61 aacaggctac atagaatcag cagataagat agggtacttt acaattcttc attcaaagcg
      121 ttatttatat gcttaccaat cgtttttata tctctaccaa ataattcagc catttgattt
      181 ctatttaacc aaactgtttc ttgttctact gcaacaggaa gcgttatcat tttatcacat
      241 tttcccacta tagtcttgtg ttttataatg agtttttatt gagaatacaa aaaggatgcc
      301 agtcaatgct tccctgatct gacatccttt actgctttat ttatattctt caggcctctt
      361 tccccttcag atattcatca atacccttcg cgcctgcctt acctgcctcc atagccagga
      421 tcacggtagc tgcgcctgtt acggcatcgc cgccggcgta tacgccttcc ttggtggtct
      481 ttccattgct ttcatctgca atgatgcatt tccacttatt ggtctgcagt ccttcggttg
      541 tagaagaaat cagcgggttt ggtgaagttc ccagagacat gatcaccgta tctacctcga
      601 tggtgtagtc ggagccttct acctctaccg gtctgcggcg tccggatgca tccggctcgc
      661 caagctccat cttacggact accatgccgg ttacccagtc attttcgtcg gtcaggatct
      721 ctaccggatt ggtaagaagg tcaaactgta cgccctcttc ctttgcgtgg tgaacctcct
      781 ccacacgggc cggaagctct gcctcgcttc ttctgtatac aacatgtacc tctgcgccca
      841 gacgaagtgc ggttctggca gcgtccatgg ctacgtttcc gccgcctact acggctacct
      901 ttttgcccag gaagatcggt gtgtcataat cgtctctgaa tgccttcatc aggttgcttc
      961 tggtcaggta ttcatttgcg gagaatacgc cgttggcatt ttcaccgggg attcccatga
     1021 acttgggaag tcctgcgccg gagccgataa atacggcctt aaagccctct tcgtccatca
     1081 gctcgtcaat ggttacggac tttccgatga tcacgtctgt ctcgatctta acgcccagct
     1141 tctttacatt ttcaatctca ggacgaacga ctctggtctt tggcagacgg aattccggga
     1201 taccgtaggt taatacgccg cccggctcat gaagtgcctc aaaaatcgtt acctcataac
     1261 ccatctttgc cagatcgccc gcacatgtca gaccggaagg gccggaaccg atgacagcta
     1321 ccttgatacc gttggtcttt tcaggctttg cgggaacgac gccgttttct ctggaccagt
     1381 ccgctacaaa acgctccagc tttccgatag aaaccggctc gcccttgatg ccgcggatac
     1441 attttccctc gcactggctc tcctgaggac atacacggcc gcagacagcg ggaagggcgc
     1501 tggactcagc aatggtagcg gctgcatccg caaatttgcc ctcttttacc tctgaaataa
     1561 attttgggat attgatggat acgggacagc cctttacgca ctgtgcgttc ttgcagttta
     1621 agcagcggga cgcctcctcc tgtgcttctt cctgtgtata gccaaggcag acctcttcaa
     1681 agttagctgc tcttactttt ggctcctgtt ctctgatggg tactctcttt aaaacgtcca
     1741 ttattcgtca cctccgcagt ttccgcagcc gccgtgatgg gtggctcctt cctgagcctt
     1801 aaggatggct ctgccttcct ctgtcttgta catctgctgg cgcttcatgg cctggtcgaa
     1861 atctaccaga tggccgtcaa actccggtcc gtctacacag gcaaatttca cctctccgcc
     1921 aacggttaca cggcaggcgc cgcacatgcc ggttccgtct accatgattg gatttaaact
     1981 tacaatggtc ggaattccca gctcctttgt aagcttgcat acgaatttca tcatgatcat
     2041 ggggccgatg gctacgcaga cgtcatactt cttgccctga ttctccacca gatccttgat
     2101 cacgtctgtt accatgccct tgcgctcata ggagccgtcg tctgtggtca catagagatt
     2161 tccggctact gcctttaatt catcctccag gatcagcaga tcctttgtct tggagccctc
     2221 gatcacatcc gcctcaatgc cgtgctcatg catccatttt acctgagggt atacgggagc
     2281 agagcctacg cctcctgcca caaagagata ctttctcttc tttacttctt cgatgtcttc
     2341 ctttacaaac tcagatggct gtcctaaggg gcctacgaaa tcctggaaat aatctccggc
     2401 ttttaattcc gccatgcgct gggtagatgc gcctacggtc tggaatacga tggtcaccag
     2461 tcccttttct ctgtcaaagt cgcagatggt cagaggaata cgctctccca cctcgtcaat
     2521 ctttacgata acaaattctc ccggctggca ggctctggct actctgggcg cctctacgtc
     2581 catgagatag atcttgtccg ccagacattc tgccttaacg atcttgtaca tgattttcct
     2641 cctctttttc actcataccc caaaaccgca gatgcgcttc taatggcatg tgtatttata
     2701 agactcagct cctcggttct attcgatccg gatgctgtag ctcttctccc aggttcccca
     2761 ggcttccagc ttctggatgc ccggccggtc ttttaattct cccttgtatc cgtcgggagc
     2821 gcacaggccg taccatggct ccaggcatac aaatggatgg gttgcctcga tggtccatac
     2881 gcccaggtat ggaaaatcgc cgcagtctat ggtcacatag ggcgtttcgt caaccagaag
     2941 gcccacgctt gaaatctgcg ccccgtcaaa catataggta aggatctggt caaagaatcc
     3001 ctttgttacc ggaacctgac cgtccttaag ctttaagctg ccggtctttt cctccacctg
     3061 atatccctgg tcattgggcg ccagatagtg aagctcctcc tgatgatttc ccgcttcttt
     3121 tccttcttta ttaaatacca gcgtataatc gtaaatggtc tttccctcag gtacctgaaa
     3181 ggccggatgg ccgccgatca taaaaagaag ctccccggaa tcgggatttt ttactctcca
     3241 catcacttca atggtccgcc ccgtaagccg atgccctatc tccagctcaa aatggaaggg
     3301 gtaaacccca taggtatccg gcgtatcctt taaccggaac cagcattcat ccatatcgca
     3361 gagcagaggt tcaaattcca tatctctggc aaatccgtgg ggcgtaatac catgctcttt
     3421 cccctcatgg gtatattttc catcaaagct ctttcctaca aacgggaaaa gtacaggcgc
     3481 atggcgattc cagactgccg gatctgctcc ccagagaaga tctctgccgg cctttttgtc
     3541 ataaatcctt gtaagctctg ccccgtgacg atccacggtg atcagcagtt cctcattttc
     3601 aagtgtaaat acggttccat cattcttcat aagtcatccc ccctttctga tgtctgcatc
     3661 attgaatctg ttgtgaaaat gtattgctgc acagacttca tattatcatt ttccaatgaa
     3721 tgttacaact gtttttaagg attttgtcac atcccggttc tcacatagcg gcaaaaagta
     3781 tagcagaggt caaaataagt ctgttcttcc ccctcctctg ccagtttcca gtccggagcc
     3841 tgatccagat ccacagggaa acgcagatct gcgtcataaa cataatcaat ggcagtgaga
     3901 tgaatggtat cgcagtaagg caggaactgc cggtaaatgc tttctccgcc caggatataa
     3961 atatcctttg gatcaaactc ctctaaatat gcaagggtct cttccatact gcggcagacc
     4021 acagcgcctc tggccttgaa ggacggatct gcggaaagaa ccacattggt acgccctccc
     4081 agaggctggc ctccgggcag tgtttccaga gttttccttc ccatcacaac caccttcccg
     4141 gcggtttcct ttaataaaag ctggcgctcc gccggaatat ttaccagtgg gcggccattt
     4201 ttcccgatcg accagtgttt atccgctgat acaataaggt tcatctgcta aatcccccct
     4261 ttcccgtccg ataccggctg ctgctcatac aggcggaact cgtagcggat cccattgtcc
     4321 tctcccgttc ccagcacttt tgtctgcttc catcccggtt cccgatccag atccggaaat
     4381 accgtatccg caggaaaggt atggaaaatg tgggtaatat aagctttttt acaataagga
     4441 agaaatgctc tgtagaccgt tccgcctcca atgataaaaa catcctcgct gtcatactgc
     4501 tttaaaagct ccagcgcttc ctccacgctg ccgcacagag ttacaccctc aggagcatag
     4561 tccgtatttc gggtgagcac aatattaacc ctgtttttta agggcttttt gcccggaaag
     4621 ctctccaggg tcttacgccc catgactacc accttttcct tcgtggtttc cctgaaaaat
     4681 ttcatatccc ccggaagatg agccaaaagc cccccatcct ttccaatggc ccagttttca
     4741 tctgctgctg ctatcaaatt catgcacata tctccaatct gccgtaatgt atccattcta
     4801 tcacatttgc ctaaaaaata cataacaaaa atctcccaga atcatcatcc tgagagattt
     4861 tattatgtat ttttcgatat gcctggcttc tggagtctgg tgtccagagc gactttgaag
     4921 gccattagaa aaccttaggg gattaaattt ttcgttaaaa gccatatcaa acctgtctga
     4981 gcgcagcgag tttttgatat ggcttttgct tttagaaaaa tttcagcccc ttagtttttc
     5041 ttatggccgg aaaccggagc tctggacacc agactccaga cctcgatgct atttcaaagc
     5101 actccctttg ccctcttttc ctcataatcc ttcagagaag ccagcgcttc tcctgccatc
     5161 ggatacagag cgatcagatt gaagatcgtc atcaggccaa cgcctacatc acccaaatcc
     5221 catacgatgg tgtaggcggc cagtccgccg ataaagagca tcaccagcgc cagaacctta
     5281 taggcggtct gggcggccca gctgtctccg aacagatagg aaacattgct gcgggcatag
     5341 tacaggattc caaggaaggt ggaaaaactg aacagccaca ggatcactgc aataaagatc
     5401 acaccgaact tgccaagatg atatcccatg gcagcctgta aaagatccat tcctgccaga
     5461 gagccggtaa tctcatccgg agctaagagc attaagaatg cggtacagct gcagattaca
     5521 atggtatcaa taaatacgcc caaagcctga accagtccca ccttggcagg atgatcggtc
     5581 tttgcggtag ctgcggcgca gggagcggag ccggaaccgg cctcattgga aaagagtccg
     5641 cgctttacac cattcatgat cactgcgccg attccgccgg ctactgcctg acgcagtcca
     5701 aaggcctctg aaaagatacg ctcaagcaca gccggaatct ggccgatatt taacagaatc
     5761 actcccactg tgatcagcag atacagaccg gccattacag gcacaatggc atccagggct
     5821 tttaccgtgg catttttacg aagaacgatc acagcgccca ggatcaccag tacaatggtg
     5881 ctgtaaagag gcggaatatt aaatgcattt tcaaaggagg aggccactga attgctgatt
     5941 acctggctga tgccgcacca gcagatcagg cctgatattg caaagagcac agaaagaggg
     6001 aaccagcggg tctgcttctt atttttctgg aaaaatttat gcatatagta ggcaggacct
     6061 ccccggtaac cgccgtaaag aggatcctct tccttataaa gctgagccag cgtagcctca
     6121 ataaaggcgg tagtggaacc cagaagggct gtgacccaca tccagaatac ggctccggct
     6181 ccgcctgcgg aaatcgcagc cacaacgcct accagatttc ccattcctac acgggtggca
     6241 gtggaaacta ccagcgtctg acaggcagaa agcgcagttg tttctccatc cttccgctcc
     6301 agagtaaccc tcagcatatc cttaaataaa cgcagggaaa ggaaacgggt ccgcagagta
     6361 aaataaatac ctgtagggat cagcaaaagc accaaaagag aaaagcttat gcctgcgccc
     6421 ccaggcagag gcagggtgat cagatctccc cacagaaact gatacaccgt ttgaattaac
     6481 gtaaccatag ttttagacac tccttctata tagaacatag attttttctg tgttccagta
     6541 tataatagtt attatcattt gtaaaattga tagtttaaat ctcaactatc tacattttcg
     6601 attgttagca ggataaagta caggagcgaa agaaatgaac atagatacga aaaatatgga
     6661 aacctttatt caggtagcgg aactgtgcag ctttacaaaa gctgccaaaa agctgggcta
     6721 ctcccagtct accgtgtcat tccagatccg ccagctggaa gaaatgctga atgtgcggct
     6781 tttggagcgg atcaatcata cggtcgcact gacagaggcc ggccggaagg tgctcaatta
     6841 tgcccatcgt ctggaacagc tgacagtgga catggaaaaa gaattaaggc cgcagccctc
     6901 tgtaagcggc catgtgcgca tggctctggc agattccctt gcctgctgga ttctgcggga
     6961 gcactatgaa aatttccgtg ctgcctatcc gggaattact ctgaaaatgg caactgcaag
     7021 cacggaggag ctgttccgga tgctgaatca aaatgaaaca gacctggtct atacgctgga
     7081 tcatcatgtt tacaacagcg actatatcat cggtgcggaa gaacggatcc aggcccattt
     7141 cgtggcagct cccggctttc ttcccttttc ctctctgact gtaccggtcc gggaactgat
     7201 cacctttcca tttctcctga cagaaaaggg gatgagctac cgccgtctga tggacgagca
     7261 tctggcccgg ttctccctgg aggtacgccc ggttctggaa tttggcagtg cagatctttt
     7321 atgtaccctt gtgactcagg gcgccggagt gtcatttttg cctgattatg taacagaagc
     7381 cgccctaaaa agcgggcggc tggtacggct ttctgtgccg gaatttaagc cggagctttg
     7441 gaggcagctt ctctaccaca aagacaaatg ggtatctccg gccatgcaga cggtgatcga
     7501 atatttttcc ggcctttccc cggcacatac ggatgcttcc gatcatatgc tgttgtaaat
     7561 ggcactttca ggagattttt atgaatcaat ctgaatcctc cactcccggc gaccgcttcg
     7621 cccagatcct gcagtttcag acacctgcgg ctctggcctg gatccgcgga aacacgccct
     7681 atgctcccgg cttaagcggt atggtgcgtt tttatgatac gccttacggc ggtgttttga
     7741 tcgaagggga atttttccgg ctccccaata agggaatttc cggctccacc gacttctacg
     7801 ccctgcatat ccatgaaaat ggagactgct ccgcgggatt cacacgaaca ggcggccact
     7861 ataatcccac aggcacatca cacccctggc attccggcga tcttctgcct gttatgggaa
     7921 acagcgggta tggatggctt tctttctatg acaagcgttt tacagtaaag gaaatcatgg
     7981 gacggtctgt ggtcctccac agcggactgg atgactttac cagccagcc
//
LOCUS       DBMDUH010000123         8016 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_4020_length_8016_cov_5.348073, whole genome shotgun sequence.
ACCESSION   DBMDUH010000123 DBMDUH010000000
VERSION     DBMDUH010000123.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 8016)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8016)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8016
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4020_length_8016_cov_5.348073"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1089
                     /locus_tag="ACZPCG_10115"
     CDS             <1..1089
                     /locus_tag="ACZPCG_10115"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR1285446.1"
                     /translation="QEQPQAATEAQETAPNTMPGSGNTTTVSTVTDVSAIVEKAMPSI
                     VAITDTMTIQQRDFFGRTQAYQTQSSGSGIIVGKNDTELLIATNNHVVAGSTDLTVTF
                     SDNKDVSAAIKGTDSATDLAIIAVKLSDIPSDTMSKIQIATMGDSDNAKVGQQVIAIG
                     NALGYGQSVTVGYVSALDRQITDENGIVHTYIQTDAAINPGNSGGALIDLQGNVIGIN
                     AAKTASTEVEGMGFAIPITSAREILDGLMTKKTREAVDEASQGYLGIQGTNIDAGTSQ
                     AYGMPVGVYVYKIVPGGAAASSDLKEKDIITKFDGQSITTMEELKDMLAYYEGGDEIT
                     LTVQSLVNGAYVEHEVSITLGTKPAANS"
     gene            1180..1512
                     /locus_tag="ACZPCG_10120"
     CDS             1180..1512
                     /locus_tag="ACZPCG_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007711114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HIR1285447.1"
                     /translation="MKLIYAIVRNDNEDDVVSQLTQHRYSVTRLSTTGGFLKKGNTTL
                     MIGAEDDKVEEVISIIKQECGQHQKLTVNMPYISGTTMVNYATMPMTVDVGGATIFVI
                     NVDRYEKI"
     gene            1702..2697
                     /gene="pta"
                     /locus_tag="ACZPCG_10125"
     CDS             1702..2697
                     /gene="pta"
                     /locus_tag="ACZPCG_10125"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008726001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HIR1285448.1"
                     /translation="MAVIDIIKEKARADKKTIVLPESMDRRTWVAAEKILKEGLANLI
                     IIGTPEDIGEHSEGLDVSGATIVNPQTYEKTQEYIDLFVELRKAKGMTPEKARETILS
                     DYAYYGCLMIKNGDAAGMVSGACHSTANTLRPCLQVIKTAPGTKLVSAFFLMEVPNCD
                     LGDNGTFVFADCGLNQNPNPEELAAIAVSSAESFKMLTGHEPKVAMLSHSSKGSAKHA
                     DVDKVVEATRIAKEMAPELALDGELQLDAAIVPEIGASKAPDSKVAGHANVLVFPDLD
                     AGNIGYKLVQRLGKAEAYGPMCQGIARPVNDLSRGCSADDIVGVVAITAVQCQNM"
     gene            2716..3912
                     /locus_tag="ACZPCG_10130"
     CDS             2716..3912
                     /locus_tag="ACZPCG_10130"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009429378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016774 - phosphotransferase activity,
                     carboxyl group as acceptor [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HIR1285449.1"
                     /translation="MKILVINCGSSSLKYQLIDMENENVIAKGLCERIGIDGSKLTHQ
                     PEGKDKFVLEQPMPNHTVAISLVMEALQDADHGVITNTDEISAIGHRVLHGGMVYSDS
                     VVVNDDVKRVIRECFDLGPLHNPANLMGIEACEEAMPGKPNVAVWDTAFGMAMPEKAY
                     MYAIPHEYFEKYSIRRYGFHGTSHMFVSGEAIKFGGLDPENAKVIVCHLGNGASVSAS
                     IGGKCVDTSMGLTPLEGLIMGTRSGDVDPAVVQFIANKENMDVNEVLNVLNKKSGVLG
                     MTNGISSDFRDIENAAKEGNHLAEVALDAFVYRVAKYVGAYTAAMNGVDAIAFTAGVG
                     ENDKATRKKVCEYLGYLGVKIDDAANDVRGKRTVISAPDSKVKVMLIPTNEELAIARE
                     TLRLVK"
     gene            4032..4559
                     /locus_tag="ACZPCG_10135"
     CDS             4032..4559
                     /locus_tag="ACZPCG_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HIR1285450.1"
                     /translation="MLINLSELFPVEGKSRTYTVPLEMTSFHAADGDYEIVEKEPVTL
                     TVTNEGNKVFTVIGEAALTLSMVCARCLEPVRVSFNLVIDQEVDMKQTGEDRIADLDE
                     QFYIKGYNLDVDQLVGNELTLNLPMRVLCSEDCKGICNRCGTNLNRGTCDCAGKSLDP
                     RMSVIQDIFKQLKEV"
     gene            4563..4745
                     /gene="rpmF"
                     /locus_tag="ACZPCG_10140"
     CDS             4563..4745
                     /gene="rpmF"
                     /locus_tag="ACZPCG_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HIR1285451.1"
                     /translation="MSICPKNKSSKARRDSRRANWKMSAPNLVKCSKCGALMMPHRVC
                     KACGSYNKREIVSVED"
     gene            4990..6048
                     /locus_tag="ACZPCG_10145"
     CDS             4990..6048
                     /locus_tag="ACZPCG_10145"
                     /inference="COORDINATES: protein motif:HMM:NF014647.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HIR1285452.1"
                     /translation="MKKLTALMLAAAMSMTALTACGSKTEESAAAPASTEAAKEEGGK
                     AAEGGEAKETSGLKVAAIFGGAINDGNWNETQYNGLKSIEEAGAEIAYMENISDTDAA
                     EAARTYASEGFNLVYLTTNSYQDYCTPVAKDFPDTTFVQINGTIVDGNFISVRIADEE
                     QGFLQGVAAALLSETGKVGFVGGQEINPIKLGSSGFQQGVDYVNSTYGLNVEATRINT
                     GSFTDVNQAKETAISIIEGGADVISPMANDASVGIMEAGEEKGVMAVGCGLGQESSAP
                     TATKIIIVKDVAIAIKSTFDAYAKGELNPTEVETYGANKGVVYATEWFGDVNDEAKTR
                     LQETIDKMAKGEVEISLQ"
     gene            6139..7224
                     /locus_tag="ACZPCG_10150"
     CDS             6139..7224
                     /locus_tag="ACZPCG_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1285453.1"
                     /translation="MQKEIVKKLKELTFPLASIVVALVLGAVILSAMGYDAGKAYSSL
                     LKGAFGSKNSIAETLIQTTPLIFTALSYAIALRCGMVNLGAEGQLYIGAILGSLAGSH
                     IHGLPPVIHILFVLLCGFIGGALWGLIVAVLKMKFGASELITTIMLNYIALEFVSFCV
                     TNPPFRDATPGAAPRMPSVVDAVKLPIILPQTRLHLGIIFALIAVLIYYVFMWKTTRG
                     YEMRVIGMNPNAGRYSGMNIPVNSILSMFLAGGFAGLGGIINIIGLQYFLTEGFSNNF
                     GFSGVAVALLGGCSPVGIIVSGILFGALNAGGVKMQLLAKVPSASIYMIQGMIIIFAV
                     SRELFIWFGRKLQKEKAHGVTGGENHG"
     gene            7217..>8016
                     /locus_tag="ACZPCG_10155"
     CDS             7217..>8016
                     /locus_tag="ACZPCG_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1285454.1"
                     /translation="MADFITNFLTADIRTATPLLLAALGLVFSERAGIVNIGTEGIML
                     VGALLGCLGAALTGSSAMGALLAIAGGFAMALIFAFFTITLRSNQIVVGTAFNIFAGG
                     FTITLNRLLLNGEKVAGYEAIPIPGLSKIPILGEVLFNWSFPVYLAFLSVPICWFVLQ
                     RTNIGLKVRAVGEYPKACDTVGINVFRIRYGAVLFSGMMAGFAGSFVSMGQLSSFTEG
                     MVSGKGFMALAVCVFGNYSPKTVLWAALLFGAADALKYRLLTTGIGSY"
BASE COUNT         2161 a         1719 c         2085 g         2051 t
ORIGIN      
        1 caggaacagc cgcaggctgc aacagaggct caggaaacgg cgccaaatac tatgccgggc
       61 agcggaaaca caaccacagt ttccactgta acggatgttt ctgcgattgt ggagaaggcc
      121 atgccgtcta ttgtagcgat tacagatacc atgaccatcc agcagaggga tttctttgga
      181 aggacacagg cgtaccagac acagagcagc ggttccggca ttattgtagg aaagaatgac
      241 acagagcttc ttattgccac caataaccat gtagtagccg gttctaccga tcttacagtt
      301 acattttcag acaacaaaga cgtttctgcc gctattaagg gtacagattc agccacggat
      361 ctggcgatca tcgctgtaaa actttcagat attccttccg ataccatgag caaaatccag
      421 attgctacta tgggagattc ggataatgca aaagtaggac agcaggtaat tgctatcgga
      481 aatgctctgg ggtatggcca gtctgttacc gtaggctatg tcagtgcctt agaccgccag
      541 atcacggatg aaaacggcat tgtccatacc tatatccaga ccgatgcggc cattaatccc
      601 ggaaacagcg gcggcgccct cattgacctt cagggaaatg tgatcggtat caatgcagcc
      661 aaaactgctt ccacagaggt agaaggtatg ggctttgcaa tccctattac cagcgcccgg
      721 gaaattttag acggtctgat gaccaagaaa acaagagaag cggtagatga agcatctcag
      781 ggctaccttg gcattcaggg aaccaatatt gatgcaggaa cctcacaggc ttatggcatg
      841 ccggtaggcg tatatgtata caagattgtt cctggaggtg cagcagcatc ttcggatctg
      901 aaggaaaagg atatcattac caaatttgac ggccagtcca ttaccacgat ggaggagctg
      961 aaggatatgc tggcctatta tgagggaggg gatgaaatta ctcttacggt acagtctctg
     1021 gtaaacggtg catacgtaga gcatgaagtg tccattacac tgggaacaaa gccggcagcc
     1081 aactcataaa aataatccgt aaaaataaaa atttttacac tatttttcag aattgtgtga
     1141 tataatagct tcaataaacc gaaaaaggag gtttggctta tgaagttaat atatgcaatc
     1201 gtacgcaatg acaatgagga tgatgtggta agccagctta cacaacaccg ctacagcgta
     1261 acccgcctgt ctaccaccgg aggtttcctg aaaaaaggaa ataccacact gatgatcggt
     1321 gcagaagacg ataaggtaga agaggttatt tccatcatta agcaggagtg cggacagcat
     1381 cagaagctga ctgtaaatat gccctatatc tccggtacga caatggtaaa ttatgcaacg
     1441 atgcccatga cggtagatgt gggcggcgca accatctttg tgataaatgt agaccgttac
     1501 gaaaaaatct gaccgccccc gcggcgtatc ggcagtgaaa gcccccggaa gagaaaaact
     1561 cattttcggg gactttttgt gttctgcggt acaggtattc aaaaaaatgc aaaaatttat
     1621 gaaaatcgac ttgtttccgg ttacgggtgc gtatataata agactatatg ctttaacata
     1681 aggaaagggg taaataagat tatggcagta atcgatatta tcaaagaaaa agcaagagca
     1741 gataaaaaaa ccatcgttct tccggagtcc atggatcgca gaacatgggt agctgctgaa
     1801 aagattttaa aggaaggact ggctaacctg atcatcatcg gtacgcctga ggatatcggc
     1861 gagcacagcg agggcctgga tgtttccggc gctaccatcg taaaccctca gacctatgag
     1921 aagactcagg aatatattga tttattcgta gagctgagaa aggcaaaggg gatgactcct
     1981 gagaaggcaa gggagactat cttaagcgat tacgcttact atggctgcct gatgatcaag
     2041 aacggagatg ccgccggaat ggtttccggc gcctgccatt ctacagccaa tactttaaga
     2101 ccctgccttc aggttattaa aacagctccc ggcaccaagc tggtttccgc tttcttctta
     2161 atggaagtgc cgaactgtga tctgggcgac aacggaacct tcgtattcgc tgactgcggt
     2221 ttgaaccaga atccgaatcc ggaagagctg gcagcgatcg ctgtttcctc tgcagagagc
     2281 tttaagatgc ttacgggcca tgagccaaag gttgccatgc tgtcccattc ttccaaggga
     2341 agcgcaaagc atgctgacgt tgacaaggta gtagaagcta cacggatcgc aaaggagatg
     2401 gctcctgagc ttgctttaga cggcgagctt cagctggatg ctgcgatcgt tcctgagatc
     2461 ggcgcttcca aggctcctga cagcaaggta gcaggccatg caaacgttct ggtattcccg
     2521 gatctggatg caggcaacat cggctacaag ctggttcaga gacttggcaa ggcagaggct
     2581 tacggcccaa tgtgccaggg cattgccaga cctgtaaacg atctgtccag gggctgctct
     2641 gctgacgaca tcgtgggtgt tgtggcaatt acagccgtac agtgccagaa tatgtaaaag
     2701 gagaaaatat ctatcatgaa gattttagtt atcaactgcg gaagttcctc tttaaagtac
     2761 cagctgatcg atatggaaaa tgaaaatgta atcgcaaagg gcctttgcga gcgtatcggt
     2821 attgacggat ccaagctgac ccaccagcca gagggcaagg acaagttcgt gcttgagcag
     2881 ccgatgccaa atcataccgt agcgatcagc cttgttatgg aagctctgca ggatgcagac
     2941 catggcgtga tcacaaacac agatgagatt tctgcgatcg gccaccgagt acttcacggc
     3001 ggtatggtat acagcgattc cgttgttgtg aatgatgacg taaagcgtgt gatccgcgag
     3061 tgcttcgacc ttggacctct gcacaaccct gcaaacctga tgggtatcga ggcctgcgag
     3121 gaggctatgc ccggaaagcc aaacgtagct gtatgggata ctgcctttgg tatggctatg
     3181 cctgagaaag cttatatgta tgcgattcct catgagtatt ttgagaaata cagcatccgc
     3241 cgctacggct tccacggaac cagccacatg ttcgtatccg gcgaggcgat caagttcggc
     3301 ggtctggatc cggagaatgc aaaggtgatc gtatgccatt taggaaacgg cgccagtgta
     3361 tctgcttcca tcggcggcaa gtgcgtggac accagcatgg gacttactcc tcttgagggc
     3421 ctgatcatgg gtaccagaag cggtgacgta gaccctgctg tcgtacagtt tattgcaaat
     3481 aaagagaaca tggatgtaaa cgaggttctc aatgtattaa ataagaagtc cggcgttctg
     3541 ggcatgacaa acggtatctc cagcgacttc cgtgatattg agaatgctgc aaaagagggc
     3601 aaccatttag cagaggtagc tctggatgca ttcgtatatc gtgtagctaa gtatgtaggc
     3661 gcttacactg cggccatgaa cggtgtggat gccattgcct ttacagccgg cgtaggtgag
     3721 aatgacaagg caaccagaaa gaaagtctgc gagtacctgg gctatctggg cgtaaagatc
     3781 gacgatgctg ctaatgatgt aagaggaaag cgcaccgtta tttctgctcc tgattctaag
     3841 gttaaggtaa tgctgatccc aaccaatgag gagctggcaa tcgcaagaga gacccttcgc
     3901 cttgtaaagt aaagctgctt gacagcggtt tttgcctgta aaaaagagtt cggtaaaaat
     3961 atggcaaaat tttgttgaca aaccccatgc acctatgtat aataacaaag gtgcatatca
     4021 aggagtatga tatgctgatc aacttatcag aactgtttcc cgttgaggga aagtccagaa
     4081 cttacacagt cccattggag atgacttcat ttcacgctgc tgacggagac tatgagattg
     4141 tggaaaagga acccgtaacg ctgactgtta caaatgaggg taataaggtg tttaccgtga
     4201 tcggagaggc agcgctcacg ctgtcgatgg tctgcgccag atgccttgaa cctgtaaggg
     4261 tttcctttaa tcttgtgatc gatcaggaag ttgatatgaa gcagaccggt gaagaccgga
     4321 ttgcagatct ggatgagcag ttctatatca aaggttataa tctggatgtc gaccagctgg
     4381 ttggtaatga gttaaccctg aacctgccta tgagagttct ctgcagtgaa gactgtaagg
     4441 gaatttgcaa tagatgtggc acaaatctca accgtgggac ttgtgactgc gcaggcaagt
     4501 cactggaccc gagaatgtca gttatccagg atatttttaa acagttgaag gaggtgtaaa
     4561 ccatgtctat ttgtccaaag aataaatctt ctaaagcaag aagagacagc cgtagagcaa
     4621 actggaagat gagcgcacca aatctggtta agtgcagcaa gtgcggagcc ctgatgatgc
     4681 ctcatagagt atgtaaagct tgcggaagct ataataagcg cgagatcgta agcgtagagg
     4741 actaatttct ccttactcaa ttaaaaataa aaagatcttc ggaagcaatt ctggagatct
     4801 tttttaattt ctgcatttcg ctttatttcc tttatttttc gtgtttttcg ttctttttta
     4861 taaaaaaaga gttaatatat tgacaatttt atccagattt gccataatag aggtgttaag
     4921 taattgatgt ttctcaaatc cccatttctt gcgaaaaagg aggggaaaaa caaaaaggag
     4981 gacagattta tgaaaaaatt aacagcactg atgcttgcgg cagctatgtc aatgacagca
     5041 ctgaccgctt gcggcagcaa aacagaggag tctgcagcag ctccggcatc taccgaggca
     5101 gcgaaggaag agggcggcaa ggccgctgag ggcggggagg cgaaggaaac ttccggctta
     5161 aaggttgcag ctatctttgg aggagcgatc aatgacggaa actggaatga aacccagtac
     5221 aatggcttaa aatccattga agaagccggt gcagagattg cttatatgga aaacatcagc
     5281 gatacagatg ctgctgaggc agcccgtaca tacgcttccg agggctttaa tctggtttat
     5341 ctgaccacca attcctatca ggattactgt actcctgttg caaaggactt cccggataca
     5401 acctttgtgc agatcaacgg aaccatcgta gacggcaact ttatttctgt ccgcatcgca
     5461 gatgaggagc agggcttttt gcagggtgtt gcagcagctc tcctgtctga gactggaaag
     5521 gttggctttg taggcggaca ggagatcaat cccattaagc tgggatcctc cggtttccag
     5581 cagggtgttg actatgtcaa ttccacctac ggcttgaatg tagaagcaac ccgcatcaac
     5641 acgggaagct ttacagatgt aaaccaggca aaggaaactg ccatctcaat catcgagggc
     5701 ggagccgatg ttatttctcc tatggcaaat gacgccagcg taggcatcat ggaagcagga
     5761 gaggagaaag gcgttatggc ggttggctgc ggccttggac aggaatcatc tgctcctact
     5821 gctactaaaa ttatcattgt aaaggatgtt gccattgcaa ttaagtctac ctttgacgca
     5881 tatgcaaagg gtgagttaaa tccaaccgag gtagaaactt atggagcaaa caagggtgtt
     5941 gtttatgcaa ccgagtggtt cggtgatgta aacgatgaag caaagacaag gcttcaggaa
     6001 accattgaca agatggcaaa gggagaggtt gagatttctc ttcagtaatt gaaccatggt
     6061 cattgaattg gagctgtcag gaacgtttct ggcagctcct tttgtcaaaa gtgcctgaac
     6121 aaaaggatgg aaaaagatat gcagaaagaa attgtgaaaa aattaaagga attaaccttt
     6181 ccgcttgcct caattgtcgt tgcgctggtg ctgggagcgg tcatactgtc agctatgggc
     6241 tatgatgcag gaaaagcgta ttccagcttg ttaaaggggg catttggaag caaaaactcc
     6301 attgcagaaa ccctgatcca gacaacccct cttattttca ctgctctcag ctatgctatt
     6361 gccctgcgct gcggtatggt aaacctgggc gcggaggggc agctttatat cggagcgatt
     6421 ctgggttctc tggccggctc ccatatccat ggacttccgc cagtgatcca tatcttgttc
     6481 gttcttctgt gcggctttat cggcggtgct ttgtggggat tgatcgttgc ggttttaaag
     6541 atgaaatttg gagccagtga gctgatcacc acgatcatgc tcaattatat tgctctggag
     6601 tttgtaagtt tctgcgtgac aaatccgcct ttccgtgatg caactccggg agctgcgccc
     6661 cgtatgcctt ccgttgtaga tgcagtgaag cttccgatca ttcttcctca gaccaggctt
     6721 catctgggta tcatctttgc tttgatcgca gtgctcattt actatgtatt catgtggaag
     6781 acgaccagag gctatgagat gcgtgtgatt ggcatgaatc ccaacgcagg ccggtattcc
     6841 ggtatgaata tccccgtcaa ctctatcttg tccatgtttt tggcaggtgg ctttgcgggt
     6901 cttggcggta ttatcaatat tatcggcctg cagtacttcc ttacagaggg cttttccaat
     6961 aactttggct tcagcggtgt tgcagtggcc cttcttggcg gctgcagccc ggtggggatc
     7021 atcgtatcgg gtattctgtt cggagccctc aatgcaggag gtgtgaagat gcagctgctt
     7081 gcaaaggttc cttctgcttc tatttatatg atccagggta tgatcattat ctttgctgtc
     7141 agccgggaac tgtttatctg gtttggcagg aagttacaga aagaaaaagc ccacggggta
     7201 acaggaggtg aaaaccatgg ctgattttat tactaacttt ctgacagcgg atatcagaac
     7261 tgccacaccg cttcttctgg ctgctcttgg actggttttc agcgagcggg ccggtatcgt
     7321 aaatatcgga acagagggta ttatgctggt aggagctctt ttgggctgtc ttggcgcggc
     7381 tcttacgggt tcctctgcca tgggagctct tttagcaatt gcaggcggct ttgctatggc
     7441 tctgatcttt gcttttttta ccattaccct gcgttccaat cagatcgtag taggaactgc
     7501 ctttaatatt ttcgcaggcg gctttaccat cacattaaac aggcttctgt taaatggtga
     7561 gaaggtagct ggctatgaag cgattcctat tcccggattg tcaaagattc ccattttggg
     7621 agaagtgctg tttaactggt cgtttccggt ttatctggct tttttaagtg taccgatatg
     7681 ctggtttgtc cttcagcgca ccaatatcgg ccttaaggta agggcagtgg gagagtatcc
     7741 caaggcctgc gatacggtgg gcatcaatgt attccgcatt cgctatggag ccgttctgtt
     7801 ttccggtatg atggcaggct ttgcaggctc ttttgtttcc atggggcagc tttcttcctt
     7861 tacagagggc atggtttccg gcaaaggctt tatggccctg gctgtgtgtg tctttggaaa
     7921 ttattccccc aagacggtac tttgggctgc gctgctgttt ggcgcggcag atgcgttaaa
     7981 ataccgtctg ctgacaaccg gcatcggaag ctatta
//
LOCUS       DBMDUH010000124         7982 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_4039_length_7982_cov_5.564522, whole genome shotgun sequence.
ACCESSION   DBMDUH010000124 DBMDUH010000000
VERSION     DBMDUH010000124.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 7982)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7982)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7982
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4039_length_7982_cov_5.564522"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(181..753)
                     /gene="sigK"
                     /locus_tag="ACZPCG_10160"
     CDS             complement(181..753)
                     /gene="sigK"
                     /locus_tag="ACZPCG_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigK"
                     /protein_id="HIR1285455.1"
                     /translation="MKTFPKPLSQMEEKECLERCKGGDQSARNMLIEHNMRLVAHVVK
                     KYQCPDYETEDLLSVGTIGLIKAVNTFNADKGSKLATYAARCVENEVLMKLRSNKKLA
                     REVSLYEPIGVDKDGETVSLMDVIEMENPETIDGLILDQDIREMYRAFDRCLTDPEKQ
                     VISMRYGLFRGREHTQREIAARMGISRSYV"
     gene            complement(817..2136)
                     /locus_tag="ACZPCG_10165"
     CDS             complement(817..2136)
                     /locus_tag="ACZPCG_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1285456.1"
                     /translation="MKDLTREHPRHTLWRFLLPMLASVMFQQFYSVADSSIAGRFAGE
                     DALAAVGASYPITVIFMAFAVGSNLGASVVVSRLFGAGDLKRMKSAVSTAFIACGVLS
                     AVLTLFGWFFCSWMMELVHTPENIFSEGELYLKIYVYGLTFLFLYNVCTGIFTALGDS
                     RTPLYFLIGSSLGNIFLDFWFVAGLGLGVAGVAWATFIAQGISAVLALVTLRFRLTKI
                     SCEGRPRLFDWVLLGQIAAIAVPSILQQSVLSVGNMFVQEIVNRYGSAVIAGYSGAIK
                     LNTFAINSFMALGSCLSSYTAQNLGAGKRERLPAGFHTGIGLSLFAALPFVILYAFFG
                     RQMMGLFLSGESKDAIEAGVLFLRIVSPMYFMIAVKLMTDGIIRGAGAMKYFVIATVP
                     DLILRICMAELLTPHFGSTGIWMAWPFGWIAATVLTLIFYRRIILEK"
     gene            complement(2284..3462)
                     /locus_tag="ACZPCG_10170"
     CDS             complement(2284..3462)
                     /locus_tag="ACZPCG_10170"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HIR1285457.1"
                     /translation="MKELELKYGCNPNQKPSRIFMENDRELPITVLSGRPGYINLLDA
                     LNGWQLVKELKEATGLPAATSFKHVSPAGAAVGLPMDEVLRKIYWVDDLGELSPLACA
                     YARARGADRMSSFGDFISLSDVCDVDTARLIKREVSDGVIAPGYEPEALEILKTKKNG
                     NYNVIQIDPDYEPEALERKQVFGVVFEQGHNNLKIDKTLLEHIVTKEKELPEDAARDL
                     LISLIVLKYTQSNSVCFAKGGQAIGIGAGQQSRVHCTRLAGSKADNWYLRQAPQVLNL
                     PFIDGIRRADRDNAIDLYIGEDYMDVLAEGRWERIFKEKPPVFTKEEKRAWLDGLTDV
                     ALGSDAFFPFGDNIDRAYKSGVKYVAQPGGSVRDDQVIETCDQYGMVMAFTGIRLFHH
                     "
     gene            complement(3494..4207)
                     /locus_tag="ACZPCG_10175"
     CDS             complement(3494..4207)
                     /locus_tag="ACZPCG_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP cyclohydrolase"
                     /protein_id="HIR1285458.1"
                     /translation="MNVRSLEQELKTNAYPGRGIVIGETGDGRYAVTAYFIMGRSANS
                     RNRVFVTEGEGIRTQAFDPEKLEDPSLIIYAPVRVLGNKTIVTNGDQTDTIYEGMDHQ
                     LTFEQSLRSREFEPDAPNYTPRISGILHTENGRYSYAMSILKSNQGDPSSCLRFTYAY
                     QNPAAGMGHFIHTYMHDGSPLPSFEGEPKAVAIEGTIDEFTSMVWNSLNEDNKVSLFV
                     RYIDIATGETETRIVNKNQ"
     regulatory      complement(4265..4366)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01750"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ZMP/ZTP riboswitches appear to regulate genes
                     involved in one-carbon metabolism; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="5-aminoimidazole-4-carboxamide
                     ribonucleotide (ZMP)/
                     5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl
                     5'-triphosphate (ZMT)"
                     /db_xref="RFAM:RF01750"
     gene            complement(4458..5843)
                     /locus_tag="ACZPCG_10180"
     CDS             complement(4458..5843)
                     /locus_tag="ACZPCG_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1285459.1"
                     /translation="MKFPDLLIMSMNNLRRRKLRTVLTVLGVIIGTAAIVVMVSLGIG
                     LNEMTMEQIASWGSLTTIEVYSQSSGGGMQMMGAAMSSQNSESEPNYITDKVIDNFKR
                     IPHVTGVSPVLNMNVVMRQGAYISTYVQLKGVSQSYLEQLELAEGRLPQPGELGLVFG
                     NGVIRDFTNAKTGKGYWDTGEMPDVDPMGKPMFVIFDMDAYYQSQGSGSSSDGTPVKP
                     PKKYMIETTGMLAGGENGYSNYSWYVFTDIDGLKAQLKKVFKKGTPIPGQPTNKKGKP
                     LNELVYNSAEVFVDDMENVTQVQEQLAAMGYQVNSQMDFLESSRQQSNMVQAVLGGIG
                     AVSLFVAAIGIANTMMMSIYERTKEIGVMKVLGCDMGNIRNMFLIESGFIGFMGGTIG
                     VALSYGVSAIVNRFVNMSQSMGLSGDLSRIPPWLSMAAIGFAVFVGMAAGFMPAVRAM
                     KLSPLAAIRNE"
     gene            complement(5830..7665)
                     /locus_tag="ACZPCG_10185"
     CDS             complement(5830..7665)
                     /locus_tag="ACZPCG_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002573309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COG1361 S-layer family protein"
                     /protein_id="HIR1285460.1"
                     /translation="MKAWKKAKALLMLAAAFMAAAGGIPSMASDFKTGYNTNASDNDH
                     IFTTKSPKGNIGKSMSISFRVRATDEDMDNLKVSLLETTEFQQIESKGESDYTVDYYP
                     FEIMETTFVGKNVGNIKAGNVKSVSLSARVRRDAAQGYYSIPIQLEWDGGYDVDYINI
                     WISTSSTTSEDEEEDKKEGNYFVIGENQSTPRGVYPNVIDFGVNFRNKRETTAQDVTI
                     SMGLSEDDAKFPFEINDGNYDRTFERVSPGETVAAQYSMAIREDSYTGYYPIKFTITF
                     RLSSEGDLHTEEGVFYVHVVSKDKEDDLGEFDANDRVRARVIVEGYHTVPEEVYAGNE
                     FELVLNMKNASTTVPASNILFNLESEKVSDSAVFTTESGTASMVVDSLAPGQTTEIRA
                     KFTAKAGVDQRSYGITVKEKYDSPEFKNAEESIIVDIPVKQYARLSTSNIDVMPDTMS
                     VGSESNVMFGINNTGKVILYNVNVTFEGDSIKPVDTYVGNIKPGETGNVDTMLTGIAP
                     TMDDGTIRLQIHYEDENGQAAEPVEKELNLMVTEEMETEFDPSLTMDPELMEPEKPSF
                     FEAYKMWILMGGAAAAGAVGFVTVRHIRKKRRAAKEKEDIEDEIS"
     gene            complement(7662..>7982)
                     /locus_tag="ACZPCG_10190"
     CDS             complement(7662..>7982)
                     /locus_tag="ACZPCG_10190"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007717989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1285461.1"
                     /translation="DQEKHMPNQMSGGQQQRVGIARALVVDPQIIFADEPTGNLDSKT
                     TMEVLRLMQRIVREQKQTLVMVTHDNNLASYADRRIRIVDGKITGIETGGRSALDETE
                     EKTV"
BASE COUNT         1956 a         2195 c         1678 g         2153 t
ORIGIN      
        1 atactgccgc agaaaattag cgatatttct aaggcaaatg aatctgaaga ttcctaagac
       61 tgtactttga caccagaagc gacgaagtta tgatgagtgg ccgctaaact taataatcaa
      121 aacactgatg cccggggagc ctatctggcc ctccgggcat cgtttacatt agtggcggcg
      181 ctatacatag gatctggata tccccatacg ggctgcaatt tccctctgtg tatgctccct
      241 gccccgaaag aggccgtacc gcatggaaat gacctgtttt tccggatctg tcaggcagcg
      301 gtcaaaggcc cggtacattt ccctgatatc ctgatccaag atcagcccgt ctatggtttc
      361 gggattttcc atctcgatca cgtccatcag acttacggtt tctccgtcct tgtctactcc
      421 gatgggttca tagagggata cctctcttgc aagtttctta ttgcttctga gcttcatcag
      481 cacttcgttt tccacgcacc gggccgcgta cgtcgccagt tttgaacctt tgtctgcatt
      541 aaatgtattg actgctttta tcagtccgat ggttcccacc gacagaagat cctctgtttc
      601 gtaatccggg cactgatatt tttttaccac atgggctacc agacgcatat tatgctcaat
      661 gagcatattt ctggcagact ggtcgccccc tttgcagcgc tccagacact ccttttcttc
      721 catctgggat aatggttttg gaaaagtttt caaagaaagc cacccctgca tattcttact
      781 tcatcatatg ccggagcagc ttgccaggtg tacgtactat ttttccaaaa tgatcctgcg
      841 gtaaaaaatc agagtgagca ccgttgctgc gatccatcca aagggccagg ccatccagat
      901 tccggtgctt ccaaaatgcg gggttaaaag ttctgccata cagatcctta agatcagatc
      961 cggcaccgta gcgatcacaa aatatttcat agccccagct ccccggataa tgccgtctgt
     1021 catcagcttt accgctatca taaagtacat ggggcttacg atccttaaaa ataatacacc
     1081 tgcttcgatc gcatctttgc tctccccact caaaaacagc cccatcatct gccttccaaa
     1141 aaaagcatac aaaatcacaa atggaagggc cgcaaacagc gacaagccga tccctgtgtg
     1201 aaatccggcc ggaaggcgct ccctttttcc tgcccccagg ttctgggccg tatagctgga
     1261 aagacagctt cccaacgcca tgaaggaatt gatggcaaag gtattgagct tgatcgcccc
     1321 ggaatatcct gcgatcaccg cagagccgta ccggtttacg atctcctgga caaacatatt
     1381 tcccacagac aggacgctct gctgaaggat gctgggaacc gcaatagctg caatctggcc
     1441 cagaagcacc cagtcaaaca gccgcggcct tccctcacag gaaatcttcg taagccggaa
     1501 cctcagcgtc acaagtgcca gtacagcgga aatcccctga gcaataaagg tcgcccaggc
     1561 cacgcctgcc actcccaggc caagtcctgc cacaaaccag aaatccagaa aaatatttcc
     1621 cagagaagaa ccgatcagaa aatacaaagg cgtcctggaa tctcccagtg ccgtaaagat
     1681 tcccgtacat acattgtaca aaaacaaaaa ggtcagtcca tatacataaa tcttaagata
     1741 gagctcacct tctgaaaaaa tattttccgg cgtatgtacc agctccatca tccagctaca
     1801 gaaaaaccag ccaaacagcg ttaaaaccgc gcttaacaca ccgcaggcaa taaaagccgt
     1861 gctgactgca gatttcatcc tctttaaatc tccggctcca aacagccgtg acaccacaac
     1921 cgatgctccc agattgcttc ctacggcaaa ggccataaat atcactgtaa tcggatagga
     1981 ggcacctact gccgccagcg catcttctcc tgcgaaccgt cctgcaatgc tgctgtccgc
     2041 aacactgtaa aactgctgaa acattacgct tgccagcatg ggaagaagaa agcgccacag
     2101 ggtgtgacgc ggatgctctc ttgtcagatc tttcattctc attatcctcg tttcatgctg
     2161 gtataatttc ccataatttt cctttttaaa tcggataaaa ggcccatgga ccatttggcc
     2221 catgagccgt gtcttttggc gggatccttt cagctgctcc gatggatgca gtacagctat
     2281 gctttaatga tggaacaggc ggattcccgt aaatgccatc accatgccgt actgatcgca
     2341 ggtttcgatc acctggtcat cccggacaga gcctcccggc tgtgctacat attttacacc
     2401 gcttttataa gccctgtcaa tattatctcc gaaggggaag aaagcgtccg agccaagagc
     2461 tacgtccgta aggccgtcca gccatgcccg cttttcctct tttgtaaata cagggggctt
     2521 ttccttaaaa atgcgctccc atcttccctc tgccagcaca tccatataat cctcgccaat
     2581 gtataaatca atggcattat ctctgtctgc ccggcggata ccgtcgataa agggcagatt
     2641 cagcacctgg ggtgcctggc gcagatacca gttatctgcc ttgctgcctg ccagtctggt
     2701 gcagtgtact ctggactgct gtcctgctcc gattccgatg gcctgaccgc cttttgcaaa
     2761 gcagacggaa ttggactggg tatattttaa gacaatgaga gaaatcagaa gatctcttgc
     2821 tgcatcctct ggaagttctt tttcctttgt aacaatatgc tcgagcagag tcttgtcaat
     2881 ctttaaatta ttgtgaccct gttcgaagac aacgccaaat acctgcttgc gctccaaagc
     2941 ctctggctca tagtccggat cgatctggat cacattgtaa ttgccgtttt tcttggtctt
     3001 taagatttct aacgcctccg gctcgtatcc gggagcgatc acgccgtctg aaacctctct
     3061 cttaatcagc cgggctgtgt ccacgtcgca gacatccgat aatgaaataa aatctccaaa
     3121 agaagacata cggtctgcgc ctctggctct ggcataggca caggctaagg gagaaagctc
     3181 tccaagatca tccacccagt aaattttcct taacacctca tccatgggaa ggcccacggc
     3241 ggcgccagca ggcgaaacgt gcttaaagga ggtggctgcc ggaagtcccg ttgcctcctt
     3301 taactctttt accagctgcc atccgtttaa ggcatccaaa aggttgatat aacccggtct
     3361 tccgcttaaa actgtaatgg gaagctctct gtcattttcc atgaaaatcc gtgaaggctt
     3421 ctgattgggg ttgcatccgt attttaattc cagttctttc atgcttctct ctcctgactt
     3481 atttttaaaa tggctattga ttcttattta caattcttgt ctctgtctca cctgtagcaa
     3541 tgtcgatata tcgcacaaag agggatacct tgttatcctc atttaagctg ttccagacca
     3601 ttgaggtaaa ttcgtcaatg gttccctcaa tggctacagc ctttggctca ccctcaaagc
     3661 ttggaagagg actgccgtca tgcatatagg tatggatgaa atggcccatt ccggctgcag
     3721 ggttctgata tgcataggta aaacgcagac aggaggaggg gtcaccctga ttgctcttta
     3781 aaatagacat ggcatagctg taacggccat tttccgtgtg aaggataccg gaaatccggg
     3841 gcgtatagtt gggagcatct ggctcaaact cacggctgcg cagagactgc tcaaaggtaa
     3901 gctgatgatc cattccctca taaatggtat ccgtctgatc accgtttgtt acaatggttt
     3961 tattgcccag cacacggacc ggagcataga tgatcaggct gggatcttcc agtttttcag
     4021 gatcaaatgc ctgagtacgg attccttctc cctctgtcac aaatacgcgg ttgcggctgt
     4081 ttgcgcttct tcccatgata aaataggcgg tcaccgcata tctgccatcc ccagtctctc
     4141 cgatgacgat cccccgtccc ggatacgcat tggtctttaa ttcctgttcc agtgatctta
     4201 cattcatgtt ttggcctcca tatataaaag taaaataaaa aaccggtctc ctggtacaca
     4261 ttgaaaaagg tgcccaggcg gccggctcgt aactgcgaac atattctgtc ccgtttcgcg
     4321 tgctccctgt ggttattccc accatccgcc agtcacacgc tcgctcattc gctttcagta
     4381 tatatgattt ctgcttatat tgcaaattaa ttttagaagc attacttatt atttctgctt
     4441 atgaaccgcc ctgtgcccta ttcgttccga atagctgcca gagggcttaa tttcatagcg
     4501 cggactgccg gcataaagcc tgcggccatg cctacaaaaa cagcaaagcc aatggctgcc
     4561 atggaaagcc atggtggaat ccgggacaga tctcccgaaa gccccatgga ctgggacata
     4621 tttacaaaac ggtttacaat cgcggaaaca ccatagctta aagccactcc tatcgttccg
     4681 cccataaaac cgataaagcc tgattcaatc agaaacatat tccgtatatt tcccatatcg
     4741 cagcccaaaa ccttcatcac accgatttcc ttggtacgtt cataaatcga catcatcatg
     4801 gtattggcaa taccgatggc ggctacaaaa agggataccg cgccgatccc gcccaaaaca
     4861 gcctggacca tgttggactg ctgtctggag ctttccagaa agtccatctg gctgtttacc
     4921 tgatagccca tagctgccag ctgttcctgt acctgggtca cattttccat atcgtctaca
     4981 aagacctctg cgctgttgta aaccagttca tttaaaggct ttcccttttt attggtgggc
     5041 tgtccgggga tgggtgttcc ctttttaaat acttttttta actgggcttt caggccgtca
     5101 atatccgtaa atacatacca ggaataattg ctgtagccgt tttcccctcc tgccagcatt
     5161 cccgtagttt cgatcatgta ctttttaggc ggctttacag gagtcccgtc tgaagagctg
     5221 ccggaaccct gactttgata gtaagcatcc atgtcaaaaa tgacaaacat gggttttccc
     5281 ataggatcca catccggcat ctctcccgta tcccagtagc cctttccggt cttggcattc
     5341 gtaaaatcac ggattacccc atttccaaat accagcccca gttctcccgg ctgcgggagc
     5401 cggccctctg ccagctctaa ctgctccaga taggactggc ttactccctt taactggaca
     5461 taggtagaaa tataggcccc ctggcgcatg accacattca tgtttaaaac aggagaaaca
     5521 cccgtcacat gaggaatccg cttaaaattg tctattacct tatctgtgat ataattcggt
     5581 tccgattcgc tgttctgaga ggacatcgcc gctcccatca tctgcatccc tccgccggaa
     5641 ctctggctgt acacttcaat ggtagtcaga ctcccccagg acgcaatctg ttccatagtc
     5701 atctcattta aaccgattcc cagggaaacc ataacgacga tcgcagccgt tcctatgatg
     5761 actcccagta ccgtcagcac cgtccgcagc ttcctgcgcc ttaaattatt catgctcatg
     5821 atcagaagat caggaaattt catcttctat atcctctttc tcctttgccg ccctgcgctt
     5881 tttacggata tgacgcacgg tgacgaaacc tacagcccct gcagccgctg ctcctcccat
     5941 cagaatccac attttatatg cttcaaaaaa ggacggcttc tccggctcca taagctcagg
     6001 atccatagta agggacggat caaactctgt ttccatttcc tccgtcacca tcagattcag
     6061 ttctttttcc accggctccg ccgcctgtcc gttttcatcc tcatagtgga tctgaagacg
     6121 gatggtgccg tcatccatag tcggagcaat gcccgtaagc atggtatcca catttcccgt
     6181 ctctccgggc ttaatattgc ctacatatgt atccacaggc ttgatggaat ctccctcaaa
     6241 ggttacgttt acattataaa ggatcacctt tcccgtgttg ttgatgccaa acatcacgtt
     6301 ggattcagag ccaaccgaca tggtatccgg cattacatca atattgctgg tgctcagacg
     6361 ggcatactgc tttacgggaa tatccacaat aatgctttcc tctgcatttt taaactcagg
     6421 gctgtcatac ttttctttta ccgtaatgcc ataggagcgc tgatccacac ctgccttggc
     6481 tgtgaattta gcccggatct ctgtggtctg gcccggagcc agagaatcca caaccataga
     6541 ggccgtaccg gactctgtgg taaaaacagc gctgtcggaa accttttccg attccagatt
     6601 aaacaggata ttcgaggccg gaaccgtagt agaggcattt ttcatattta atacaagctc
     6661 aaattcattg cccgcataga cctcctccgg caccgtatga tatccctcta cgatcactct
     6721 ggcccggact ctgtcatttg cgtcaaattc ccccaggtcg tcctccttat ccttggacac
     6781 cacatgaacg taaaatacgc cctcctctgt gtgaaggtcg ccctcagagc ttaagcggaa
     6841 ggtaatggta aatttgatcg gataatagcc tgtataggaa tcctcacgga tggccatgct
     6901 gtactgggca gccacggttt ctcccggact gacccgctca aaggttcgat cataattgcc
     6961 gtcattgatc tcaaagggga atttggcgtc gtcttccgaa agccccatgg aaatggttac
     7021 atcctgggct gtggtttctc ttttattgcg gaaattgacg ccaaaatcaa tgacattggg
     7081 ataaactccc ctgggtgtgg actgattttc accgatcaca aaataatttc cctctttttt
     7141 atcctcttcc tcatcctcag aggtagtgga actggtggaa atccagatat tgatgtaatc
     7201 cacatcatag cccccatccc actctaactg aatggggatg ctgtaatagc cctgggcggc
     7261 gtctctgcgg actctggcgg aaagggaaac actttttaca ttgcccgcct ttatatttcc
     7321 tacatttttc cccacaaagg tggtttccat aatttcaaat ggatagtaat caactgtata
     7381 gtcgctttca ccttttgact cgatctgctg aaattccgtt gtttcaagaa ggcttacctt
     7441 tagattatcc atatcctcat ccgtcgcccg aacccggaat gaaatgctca tagacttgcc
     7501 gatattcccc ttgggagact tggttgtgaa tatatgatca ttatcactgg catttgtgtt
     7561 gtagcctgtc ttaaaatcac tggccataga cgggatgcct cccgctgctg ccataaaagc
     7621 cgctgcaagc ataagcaggg cctttgcctt tttccatgct ttcatactgt tttctcctcc
     7681 gtctcatcta aggctgaccg gcccccggtc tctatgcccg tgatcttccc gtccacgatc
     7741 cggatcctgc ggtctgcgta ggaagccagg ttgttgtcat gggtaaccat cacaagtgtc
     7801 tgcttctgtt ctcttacaat gcgctgcatt aaccgcagta cctccatggt ggtcttggaa
     7861 tccagattcc ccgtaggctc atccgcaaaa atgatctgcg ggtctaccac cagagccctg
     7921 gcgatgccca ctctctgctg ctggcctccg gacatctggt ttggcatatg cttttcctga
     7981 tc
//
LOCUS       DBMDUH010000125         7908 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_4074_length_7908_cov_5.494044, whole genome shotgun sequence.
ACCESSION   DBMDUH010000125 DBMDUH010000000
VERSION     DBMDUH010000125.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 7908)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7908)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7908
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4074_length_7908_cov_5.494044"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1796)
                     /locus_tag="ACZPCG_10195"
     CDS             complement(<1..1796)
                     /locus_tag="ACZPCG_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR1285462.1"
                     /translation="MVNITKFAVRKPVTIIMCLITIAYFGIQALMGTKVELTPEMELP
                     VLLISTVYAGATPEDINDLVTTKQEDAITSLDGVKNVYSFSQENVSIIMVEYEFGTNI
                     DTAYINLKKAIDGTKRDMPEDVEDPNILEMDMNASPVMTLAVGGQVDGNLYTYVENNV
                     QPELEKLSSVGEVSLAGGQKEYVSVCLDPEKMAQYNLSLSTIGQLVGAADFAIPAGDV
                     NVGKQELNVSAGNDYDDVESLNNVAIPLADGNVIHLSDIATVSQTLEEENSIGRYNGK
                     DVVSIAIKKQQSSTAIDVSRQVTKQIEKLKAANPGLEFTVVNDSSDMINESIVNVYQT
                     MFMAIILSMFILWLFYGDLRASVIVGTSIPISIMLTLIAMSFMGFSLNVISLTSLVLG
                     VGMMVDNSINVLDGCFRAKEKMNFYDAAIEGSRVMINSIIGGTATTCVVFIPLSLLSG
                     MSGQLFKQLGFTIVFSLIASLFSAVTIVPLCYYQWHPIEKEKAPVAGFIRGCQEWYRA
                     HMPSIIPKTGMIIGGSLLLLVASFGLASQLRMELMVAVDEGIVDMEIKTKPGLSIAAV
                     NEAVAEVEQLVAQDEEVDHYLLTYGASGLSMGG"
     gene            complement(1800..2927)
                     /locus_tag="ACZPCG_10200"
     CDS             complement(1800..2927)
                     /locus_tag="ACZPCG_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR1285463.1"
                     /translation="MKKSSKRLIAGAVVLLVAAGFVFKLVSGKEEAPQYETRPTVAVQ
                     TPVTGDITLYTELTGTVEPVSRASVMPKISGEILEVNFQAGDTVQAGQVLCKIDSDAL
                     RTMELQMQSASVAADTAARELARLQPLFDGGFVSQQQFQQAQDAATSARLAYETAKTQ
                     YDLQMEYTTVTAPIGGVIESRGIELHDHVSPSAPICVISGGDQLQVNFGVTEKVMKNM
                     NVGDALTVEKNGSTYEGNLTEIGAMVNASGLYDAKAAVSQGASLTNGAKVKLTVVMDR
                     AEGAMTVPVDAVNYDNGNAFVYCYEDGTAKKTMVEAGIYDSQNMEIISGLEADSQVIV
                     TWSNELVDGQQVLLDDGQKEESSEGNADQETSAAESEKAGE"
     gene            complement(2961..3578)
                     /locus_tag="ACZPCG_10205"
     CDS             complement(2961..3578)
                     /locus_tag="ACZPCG_10205"
                     /inference="COORDINATES: protein motif:HMM:NF012655.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR1285464.1"
                     /translation="MPTEKFMNLGDYKKQAILDAMRAEFMTTPYSEIRISSLIKRAGI
                     SRASFYLYFQSKEDLFTCMMHQLRERLVQGLVEAFQEQKSSFDASMSRLVNRIMDDEI
                     YRDCWEFCKRVMEDGTCREVAACAEREYDKEDLREKTEKKCYEAMDHSGYPGLDKEKL
                     RYAIGMGMLVILRISMLYLDHAADPKRLKEMAARQFAILDRGIRD"
     gene            complement(3727..4329)
                     /locus_tag="ACZPCG_10210"
     CDS             complement(3727..4329)
                     /locus_tag="ACZPCG_10210"
                     /inference="COORDINATES: protein motif:HMM:NF025636.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR1285465.1"
                     /translation="MASFTKFAILKVFGDLAASRPVDKITVKDITDQCGISRNTFYYH
                     YQDIYQVLKAYVQYSAEHVIELMVEDEGEDGKAGLKEIRYLEANRELLCNLYRSAANE
                     EVRNCLQSASQIIFDRLIESVSQGMEVQAEDKKILSAVCQYTVRGIMTSWMEEDGMLN
                     GETLEQVLVRLDYLFKGAIREALMRSASREQGLLPESKML"
     gene            complement(4397..5317)
                     /locus_tag="ACZPCG_10215"
     CDS             complement(4397..5317)
                     /locus_tag="ACZPCG_10215"
                     /inference="COORDINATES: protein motif:HMM:NF025616.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HIR1285466.1"
                     /translation="MKKRFVGLLAVVLVLFGFTACGGAASGAAADKMEIAETMAVSPP
                     AAAGGHMPEAPALEETERETGETLTSRSQTRKLIRTVELQMETGEFDAFLQAVGEKTE
                     TFGGYTERSDVMGKQADSEGNPILRTASLAVRIPTGRLDEFITDLEGKGSIVNRSENT
                     EDVTLQYSDIESRKKTLEMEQDRIWALLEKADTLDAVIALEKRLSEIRYDLESMESQL
                     RLYDNQVEYSILRLDLSEVTNYSPTEPQTIWQRIRTGFVENAHRAAGLFSSLFVGIII
                     SVPFWVPAAAILFIFYFIGRKYAKKHGEKK"
     gene            5526..5837
                     /locus_tag="ACZPCG_10220"
     CDS             5526..5837
                     /locus_tag="ACZPCG_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HIR1285467.1"
                     /translation="MEAKNPAACSCHKTKERSEKEYKDLIHRLNRIEGQVRGIKSMVE
                     KNAYCPDILIQTAAVTAALNSFSRTLLSSHIHTCVVNDIREGNDQVIDELMNTLQKLM
                     K"
     gene            5855..>7908
                     /locus_tag="ACZPCG_10225"
     CDS             5855..>7908
                     /locus_tag="ACZPCG_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR1285468.1"
                     /translation="MKQYSVTGMSCAACSARVEKAVNHVPGVTSCSVSLLTNSMGVEG
                     NADPKAVIAAVEQAGYGASEKGISDHASARTSENEDMLADHETPILKKRLLCSAGFLI
                     VLMYFSMGHMMWGWPVPPFFADNHVAVGILQLLLTGIVMVINQKFFINGFKSLFHGSP
                     NMDTLVALGSTAAFVYSTYALFAMTGAQLQGDMDGVMTYMHEFYFESAAMIVTLITVG
                     KMLEARSKGRTTDALKGLMKLAPKTATLIKNGAETEVSIDQVQIGDIFVVRPGENIPV
                     DGIVLEGNSAVNESALTGESIPVDKSQGDRVSAATSNQSGFLRCQATRVGEDTTLSQI
                     IQMVSDAAATKAPIAKAADRVSGIFVPAVITVSIITTIVWLLAGQGLGFALARGISVL
                     VISCPCALGLATPVAIMVGNGMGAKNGILFKTAVSLEETGKMQIIALDKTGTITEGQP
                     KVTDIVPADGYTDGSLLSLAHALEAKSEHPLAKAVLKRAEENQMEPEAVTDFKALPGN
                     GLSAVRTRDHHVLTGGSLSFISSRTPISQNMKKQAEALAEEGKTPLLFTENDRPAGII
                     AVADTIKEDSPQAIKELKNMGIHVVMLTGDNERTARAIGRQAGVDEVIAGVLPEGKES
                     VIRTLKKKGKTCMVGDGINDAPALTRADMGIAIGAGTDIAIDAADVVLMKSRLLDVPA
                     AIR"
BASE COUNT         1974 a         2130 c         1699 g         2105 t
ORIGIN      
        1 cctcccatgc tcagaccact ggctccgtag gtcagaagat aatggtctac ctcctcgtcc
       61 tgtgctacca gctgctctac ctcagctact gcttcattta ctgcagcaat ggaaaggcct
      121 ggttttgtct tgatctccat atccacaatg ccttcgtcta ccgctaccat cagctccatt
      181 ctaagctggg aagcaaggcc gaaggaggcc accaaaagca gaaggcttcc gccgatgatc
      241 atacctgtct ttggaatgat ggaaggcata tgtgcacggt accactcctg acagccgcgg
      301 ataaagcccg ctacaggagc cttctctttc tctatcggat gccactgata atagcacaga
      361 ggcacgatgg tcacggcaga gaataaagat gcgatcaaac tgaatacgat ggtaaatccg
      421 agctgtttga atagctgtcc agacataccg ctcaacaggg aaagcgggat aaatacaacg
      481 caggtggtcg ctgtaccgcc gatgatggaa ttgatcataa cacggcttcc ctcgatggcc
      541 gcatcgtaga aattcatctt ttcctttgcg cggaaacatc cgtccagtac gttgatggag
      601 ttatctacca tcatacctac gcccagaacc agagaagtca atgaaataac gttcagcgag
      661 aatcccataa agctcatggc gatcaaggtc agcatgatag aaataggaat agaagtacca
      721 acgataacgg aagcacgcag atctccgtag aacagccaca ggataaacat ggacaggata
      781 atcgccatga acatggtctg gtatacgttg acgatggatt cgttgatcat atcgctggaa
      841 tcatttacaa ccgtaaattc cagtcccgga ttggctgcct tcagcttttc aatctgcttt
      901 gttacctgtc ttgatacatc aattgccgtt gagctctgct gctttttaat ggcaatcgaa
      961 acaacatcct ttccgttata acggccgatg gaattttctt cttccagtgt ctgggaaacg
     1021 gtcgcaatat ctgacagatg gattacattg ccgtctgcca gagggatagc aacattattc
     1081 aggctttcca catcgtcata atcgttgccc gcgctgacgt tcaattcctg ctttcctacg
     1141 tttacatcac cggcaggaat agcgaaatcc gctgcgccta ccagctgtcc tatcgtagaa
     1201 aggcttaagt tatactgggc catcttttca gggtccagac aaacgcttac atattccttc
     1261 tgtccgccgg caagggaaac ttcacctacg gagcttaatt tttccagttc cggctgaaca
     1321 ttgttttcta catatgtata aaggtttccg tctacctgtc cgccgactgc aagcgtcata
     1381 acaggagaag cattcatatc catctcaagg atattgggat cttccacatc ttccggcata
     1441 tcccgcttgg ttccgtcaat ggcttttttc aggttgatgt atgccgtatc aatattggtt
     1501 ccgaactcat actccaccat gatgatggaa acattttcct gggaaaagct gtatacattt
     1561 ttaacgccgt ccagtgaggt aatggcatct tcctgtttcg ttgttacaag gtcgttgata
     1621 tcctcagggg tagcgcccgc atatacggta ctgatcagca gaacaggaag ttccatttcc
     1681 ggcgtcagct ctaccttcgt ccccatcaga gcctgaatac cgaaatatgc aatggtaatc
     1741 aggcacatga tgatggttac aggcttgcgt accgcaaatt tagtaatatt aaccattcct
     1801 tattcacctg ctttctctga ctctgcggca gatgtttcct gatccgcatt tccttcgctg
     1861 ctttcttcct tctgtccgtc atccaaaagc acctgctgtc cgtctaccag ctcattgctc
     1921 caggttacga tcacctggct gtctgcttcc agtccggaaa taatttccat attctgtgaa
     1981 tcataaatac cggcttctac catggttttt ttagcagtgc cgtcttcata gcagtataca
     2041 aaagcgttac cgttgtcata attgaccgcg tctacaggaa ccgtcattgc accctctgcg
     2101 cgatccatta caacggtcag ctttaccttt gctccgtttg taagactggc tccctggctg
     2161 acagctgcct ttgcatcgta taagccggat gcatttacca tggctcctat ttccgtcaga
     2221 ttgccttcat aggtgcttcc gttcttttct accgtcaaag cgtcgcctac gttcatattt
     2281 ttcattacct tctctgttac cccgaaattc acctgaagct ggtctcctcc ggaaattacg
     2341 cagatagggg cagaagggct tacatggtca tgaagctcaa tccctctgct ctcgatcacg
     2401 ccgccgatcg gagcggtcac tgtggtatat tccatctgaa ggtcatactg ggtttttgcc
     2461 gtttcataag ccagacgggc gctggtggcc gcatcctgag cctgctggaa ctgctgctgg
     2521 gaaacaaatc cgccgtcaaa caaaggctga agacgcgcca gctctctggc tgccgtatct
     2581 gcggctacgg atgcggactg catctgaagt tccatggtcc ttaacgcatc tgaatcaatt
     2641 ttacacagca cctgaccggc ctgtaccgta tcgcctgcct ggaaatttac ctctaagatt
     2701 tcgccgctga tctttggcat tacagacgct ctggatacag gctctacggt tcctgtaagt
     2761 tcggtataga gtgtaatgtc tcctgttacc ggtgtctgta cggctaccgt aggacgtgtt
     2821 tcatactgcg gggcctcctc tttgcctgac accagtttaa atacaaaccc tgccgccact
     2881 aaaaggacta ccgctcctgc gatcagtctt ttgctgcttt ttttcatttt tttctgcctt
     2941 cctttctttt atacacgatt ttaatcccgg attcctctgt caagtatggc aaactgcctg
     3001 gctgccatct ccttcagcct ttttggatct gccgcatggt ccagatacag catggatatc
     3061 ctgaggatta ccagcatccc catgccgatg gcataccgca gcttttcctt atcaagccct
     3121 ggatatccgg aatgatccat ggcctcataa catttttttt ctgtcttttc ccgcaggtct
     3181 tctttgtcat attcccgctc tgcgcaggct gccacctctc tgcaggtccc atcctccatc
     3241 acccgcttgc agaactccca gcagtctctg tagatctcat catccataat ccggtttacc
     3301 agccggctca tggacgcatc aaatgaactt ttctgttcct ggaaggcttc taccagcccc
     3361 tgcaccagcc gctctctcag ttgatgcatc atacaggtaa acaaatcttc tttgctctgg
     3421 aaatacagat aaaaactggc tctggatatg cctgcccgct tgatcaggct gctgattcgg
     3481 atctccgagt aaggcgtagt catgaattct gcgcgcattg catccaggat tgcctgtttc
     3541 ttgtaatcac ccagattcat gaatttctct gtgggcattt ttttcatctc ctttaccatt
     3601 aatcattata tggacttttg tctaattgac aataggcaaa ataaaatcaa atgcacaaaa
     3661 tttgtgcatt tgacccatat gtgtactatt ttttactttt atttcacaat ttacagcaga
     3721 cttgctttac aacattttgc tttctggcag aagcccctgt tctctggacg cgctccgcat
     3781 caacgcttct cttatggccc ctttaaacaa atagtccagg cggaccaaaa cctgctccag
     3841 tgtttccccg ttcagcattc cgtcctcttc catccagctg gtcatgatcc cccgaaccgt
     3901 atactggcac accgcactga gtatcttttt atcctctgcc tgtacctcca ttccctgaga
     3961 tacgctttca atgaggcggt caaaaataat ctggctggca gactgcaggc agtttctgac
     4021 ctcctcgttg gcagcagagc gatacagatt gcataaaagc tctctgttgg cttccagata
     4081 ccggatttcc tttaagcctg ccttaccatc ctccccctca tcctctacca tcagctcgat
     4141 cacatgctca gccgaatact ggacataggc tttgagcacc tggtaaatat cctgataatg
     4201 gtaataaaac gtatttctgg aaataccgca ttgatctgta atatccttta ccgtaatttt
     4261 atctaccggt ctggaagctg ccagatcccc gaacactttc aagattgcaa atttcgtaaa
     4321 tgatgccatg ggcagacatg tcctccttta attaaatagg agtccattat accgtaaaat
     4381 ggtccaaact gcaaattcac ttcttttccc catgtttttt cgcatatttt cggcctataa
     4441 agtaaaagat aaaaagaatc gccgccgcag gaacccaaaa cggaaccgat attatgatcc
     4501 ctacaaaaag agaagaaaac aaacctgctg ccctgtgggc attttccaca aatccagtgc
     4561 ggatcctctg ccatatggtc tgaggctctg tgggactgta atttgttact tctgacagat
     4621 ccaaacgcag aatactgtat tccacctgat tatcatacaa gcgcagctgg gattccatgg
     4681 attccagatc atagcggatc tcagacagcc gtttttccaa ggcgatcact gcatccagag
     4741 tatcagcttt ttccaaaaga gcccagatcc tgtcctgctc catttccaac gtctttttcc
     4801 tgctctcaat atcactgtac tgaagggtaa cgtcctctgt gttttccgaa cgattcacta
     4861 tacttccttt tccttccagg tctgtaatga attcatccag acggcccgtc gggatcctga
     4921 ctgcaaggct ggcagtacgc aaaatgggat ttccttcgga atctgcctgt tttcccatca
     4981 catctgaccg ctccgtatat cccccaaaag tctctgtctt ttccccgacc gcctgaagaa
     5041 acgcgtcaaa ttctcctgtc tccatctgaa gttctaccgt gcggatcagc tttcgtgtct
     5101 gagaccggga tgttaacgtt tctcccgttt cccgttctgt ttcttccaaa gcaggagctt
     5161 ccggcatatg tcctcctgca gctgccggag gactgacagc catcgtttca gctatctcca
     5221 ttttatccgc agctgcaccg gaagcagcgc ctccgcaggc tgtaaaccca aacaggacaa
     5281 gcaccaccgc caataatccc acaaatcttt ttttcatgct cttctcctcc tttcctttct
     5341 gatcccaaca tatcatattt tttctttcct ctcaatgtcc ctgagaaaat cgtaagagga
     5401 acgacagctt ttgttatatt tttttaatat tcctcttgca acatacccca tcagggtata
     5461 taatatcact ataccccacc agggtatatt ctcatcccaa tcccgtatca gacaggagga
     5521 cttatatgga agcaaaaaat ccagcagcat gcagctgcca taagacaaaa gagcgttccg
     5581 aaaaagaata taaagatctt atacatcgct taaaccggat cgaaggacag gtgcgcggca
     5641 tcaaaagcat ggttgaaaaa aatgcctact gcccggatat actgatacag acagccgccg
     5701 ttaccgctgc tctcaacagc ttcagccgca cccttctttc cagccatatc cacacctgcg
     5761 ttgtaaatga tatcagggaa ggaaacgatc aggtaatcga cgagctgatg aacaccctgc
     5821 agaaactgat gaaataaagg aggcattgat gcttatgaaa caatattccg taaccggcat
     5881 gagctgcgcg gcctgcagcg cccgggtgga aaaagccgtg aatcacgtcc ccggcgttac
     5941 ttcctgttcc gtgagccttt tgacaaattc catgggcgta gaaggaaacg cagatccaaa
     6001 ggctgtcata gccgcggtag agcaggcagg atacggcgca tctgaaaagg gcatatcgga
     6061 tcatgcttct gcccggacat ctgaaaacga agatatgctg gctgaccatg aaactcccat
     6121 actgaaaaaa aggctgctct gttccgcagg ctttctcatt gtcctcatgt atttttccat
     6181 gggccatatg atgtggggct ggccggtgcc cccatttttt gcagataatc acgttgcagt
     6241 agggattttg cagcttcttc ttacaggaat tgtaatggta atcaaccaga aattttttat
     6301 caacggcttt aaaagcctgt ttcacggctc tcccaacatg gataccctgg tagccttagg
     6361 ctccacagcc gcttttgttt acagcaccta tgcccttttt gccatgaccg gagcacagct
     6421 tcagggcgat atggacggcg tcatgaccta tatgcatgaa ttttattttg agtccgccgc
     6481 tatgatcgtc accctgatca ccgtaggcaa gatgctggag gcacgctcca aggggcgcac
     6541 caccgacgcc ctaaaaggcc ttatgaaact ggctcccaag acggccacgc ttataaaaaa
     6601 cggagctgag acagaagtct ccatcgacca ggtacaaatc ggggatattt tcgttgtacg
     6661 tcccggagaa aatatacccg tagatggtat tgtattagaa ggaaacagcg cagtaaacga
     6721 atctgctctt acaggagaaa gcatccctgt tgacaaatca cagggtgacc gggtatctgc
     6781 cgctacctcc aaccagtctg gctttttacg ctgccaggcc acacgggtag gagaagatac
     6841 caccctctcc cagatcatac agatggtaag cgatgctgct gccaccaaag ctcccattgc
     6901 caaggcagca gaccgggtat ccggcatttt tgttccggca gttatcactg tttccattat
     6961 taccacgatc gtatggctcc ttgcaggcca aggtctgggt tttgcactgg caagaggaat
     7021 ctctgtactt gtcatcagct gcccctgtgc actgggactt gcaacccccg tagccattat
     7081 ggtcggaaac ggaatgggcg ccaaaaacgg aattctcttt aaaacggctg tttctctgga
     7141 agaaacagga aagatgcaga tcattgccct ggataagaca ggcaccatca cagagggaca
     7201 gccaaaggta acggatatcg ttcccgcaga cggctataca gacggctctc ttctttccct
     7261 ggctcacgcc ctggaggcca aaagcgaaca tcctctggca aaagccgtgt taaagcgggc
     7321 cgaagaaaac cagatggagc ctgaggctgt aacagacttt aaggcacttc ccggaaacgg
     7381 gctttctgct gtccgcacca gagatcacca tgtacttacc ggaggaagcc tttcctttat
     7441 cagcagccgt acgcccattt cacaaaatat gaaaaagcag gccgaagcgc tggctgagga
     7501 gggcaaaaca ccgcttctct ttacggaaaa tgatcgtcct gcaggtatca ttgccgtggc
     7561 agacaccatc aaggaggaca gcccacaggc aatcaaggag cttaaaaaca tgggaatcca
     7621 tgtggtaatg ctcacaggag acaatgagcg gacagccaga gccattggcc gccaggccgg
     7681 agtggacgag gtaatcgcag gcgttctccc cgaagggaag gaaagtgtga tccgcacctt
     7741 aaagaaaaag ggcaaaacct gcatggtagg cgacgggatc aacgatgctc ccgctctcac
     7801 aagggctgat atgggaattg ccataggagc cggaaccgat atagccatcg acgcggctga
     7861 tgtggttctg atgaaaagcc gcctcttaga cgttcctgcg gccatacg
//
LOCUS       DBMDUH010000126         7887 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_4090_length_7887_cov_7.963653, whole genome shotgun sequence.
ACCESSION   DBMDUH010000126 DBMDUH010000000
VERSION     DBMDUH010000126.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 7887)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7887)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7887
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4090_length_7887_cov_7.963653"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="ACZPCG_10230"
     CDS             complement(<1..917)
                     /locus_tag="ACZPCG_10230"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1285469.1"
                     /translation="MLHSHSPLPREPFHLKQSVVETVRTLPTTIIYMAAATLVSSLMF
                     HFTDNLINVSIVYILAIILHARATSCYGAGIIASLYSVFWVNYAFSVPYMKLNFTLSG
                     YPLTFFGMAFISCFTSSICIMISRQTNLIREQDKKLMNAEKETMRANLLRAVSHDLRT
                     PLTTIIGSSVACLEQQNTMTAEEQRQLISNIEEDAQWLLNMVENLLSVTRIQDTDGAP
                     AVVKTEEPLEEVISEAVQRFRKRFPLAVVHVSVPDAFIMVPMDAKLIEQVINNLLENA
                     AFHGNSQEPIELTAAIHDKILSVTIKDYG"
     gene            complement(957..2540)
                     /locus_tag="ACZPCG_10235"
     CDS             complement(957..2540)
                     /locus_tag="ACZPCG_10235"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR1285470.1"
                     /translation="MKRLFPFFICLSLILSFTPITAAAAVPWPTGVSIEADGGILMDA
                     DTGSILYGKNENQAYYPASITKILTALIVLEECELDEMVTFSHDDVYNVEAGSSSAGI
                     DEGDVLTVRDCLYALLLASANESANALACHVSGSREAFAELMNQKARSLGCTGSNFAN
                     PSGLNDENHYTTAHDMALITQEAIKNPDFVEISGTRSYQMAPTKKNAEGGYVANHHNM
                     VKQNTSVTYPGAFAGKTGYTSLAGNTLVTCAERDGITLIAVVLNGHQSHYRDTKALFD
                     FGFSNFQSLKAADHETTYRSLENDMTIAGMTAKDSISLSLDPDSRVILPREAVFSDLT
                     PSLSYELGQDAPKHAIARISYTYEGHETGQVYLCTTAQVNRISAPDTVISSDAALSES
                     SDAANLSTAPSKASVPSETESPDPVLQIQRRSAQPDAAGTEDKGTLFGLPLWLTVSLA
                     GGFAVILGAGIFLFLHNRQKEQEDLMRRRQRRLERLEDMGFSSSDFDQMLSKKRSSIP
                     SYRPRRRTKGRSFRKNRRR"
     gene            complement(2552..3244)
                     /locus_tag="ACZPCG_10240"
     CDS             complement(2552..3244)
                     /locus_tag="ACZPCG_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HIR1285471.1"
                     /translation="MKYLHRLTGILMAFCLMIVLFITSIEAVVYWTPGYFEKEYTKYE
                     VLKDLPAMTMEDLLEVTDEMMDYLRGDREDLHVMTVMDGQEREFFNAREIAHMEDVQG
                     LFLAAISIRRVCLVLCASALVFMAVTKADMKRLLPPSVCLGTGLFFGLIAALAAIIST
                     DFTKYFIMFHHIFFSNDLWILDPSTDMLINIVPEGFFMDTAGRIAFTFGSLSLILFAV
                     CLALTLKHKHRP"
     gene            complement(3523..4851)
                     /locus_tag="ACZPCG_10245"
     CDS             complement(3523..4851)
                     /locus_tag="ACZPCG_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HIR1285472.1"
                     /translation="MKTFKLVIAGGGSTYTPGIVKSLLVQKDRFKLNELVLYDNNAKR
                     QEDVGVIVRHVVEMFDPDVKLTLTTDPETAMTDADFIFAQMRVGLYAMREQDEKIPLR
                     YGVVGQETCGPGGLAYGLRTIYPMVELIDYCEKYAKPTYWIVNYSNPAAIVAKATFRL
                     RPHARILNICDMPVAIERNMAEILGCDRHDLEVDYFGLNHFGWFTKVRLNGTDVTEEL
                     KSYVAENGYMPKNEKSDVMHSDPSWLHTYANSRHICSAFHDYLPNTYMQYYLLGDEVV
                     ESSNPNHTRANEVMEGREKRIFDAVADYRAGKEVDLTKFFGGVHGEFIVDVAMSLAFD
                     LRKRYLVMVENRGAIENLPDDAMVEIPAYITDRGAEPVRVGEIPTFYKGLIEQQEAVE
                     KLVVEAAIEHSYEKALMAFTLSKTVPSLLVAKKILDDMIEANKEFWPELH"
     gene            complement(4881..6473)
                     /locus_tag="ACZPCG_10250"
     CDS             complement(4881..6473)
                     /locus_tag="ACZPCG_10250"
                     /inference="COORDINATES: protein motif:HMM:NF014437.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HIR1285473.1"
                     /translation="MKAKVMNAMQSFSKALMGPVLFLPIAGMLQALSSVMCNTAFVSE
                     GGILYTAGKFINGGVSAVIGNLGILFCVGVPMSLAKKKKADAAFVSLVSYLIWLAANS
                     RWLSVAGLMAEGDTASALYGTGQTICLGYHVTDMGVFLGMILGVIVALVHNRFIDTEF
                     KGAMAMYGNSKFVFIVLLPIVLVLALAAAYIWPVAAAAINALTGIMAGAGAFGVFLYG
                     FLNRILIPTGLHHLVWSPFLYSAIGDSMIIGGEQIVGAKPVFLALLSDPAVASMPDSA
                     RFLTYGLMKTFGVIGVALAFIATAKKEKKAATKAQVIPATLTASLVGVTEPLEFTFLF
                     AAPMLWVIYSVMDGLFQMIVYLLNVRVCATNGIIDFLVLNLPAGIGRTHWPMYVVAGL
                     IEIVVMYFLFRFLIVKFNLKTPGREDGEDVIDLAANAAAVKSQIRSGNKAEAKKEADA
                     ETAARIIEGLGGPDNIENIGNCMTRLRVEVKDASVVKDKEWFAPTGAAGLVVKGNNIQ
                     IIYGPVVSKKRSIVEKALGREE"
     gene            6725..7450
                     /locus_tag="ACZPCG_10255"
     CDS             6725..7450
                     /locus_tag="ACZPCG_10255"
                     /inference="COORDINATES: protein motif:HMM:NF013577.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HIR1285474.1"
                     /translation="MKHDIYKLAEQYHLNESEQQMFQSLLEAADQNIQYSVREFAARN
                     YVSPAALIRLSKKMGYTGYTDMVYRLGFLVQNEINNKNHESDITAFIGEIPFEKMNRF
                     PELLKKHRHNPILITGTGFCAPLRDFFVRKLLVMGYCAIGSNNYEVYEGNALSAGMVI
                     AISKSGTTDTIIKPVRDACAHGIDVVAFTGGAHSMIADMAELSFVLLDDKILDDRNLT
                     ANYFYARVLILFEYLMDMTWETE"
     gene            complement(7546..>7887)
                     /locus_tag="ACZPCG_10260"
     CDS             complement(7546..>7887)
                     /locus_tag="ACZPCG_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009244493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1285475.1"
                     /translation="IFFPGPILRIFDADPELMQYGTEALRMIGPSFLISSVAFIAAGV
                     FEALGQGRPSLIISLLRQLVIIVPLGWILSRFMGPAGIWISFPIAELIAAVIAWKMMG
                     KIYADHPENTH"
BASE COUNT         2106 a         1731 c         1963 g         2087 t
ORIGIN      
        1 tttccatagt ctttgatcgt gacagaaagg attttatcat gaatggcagc tgtcaattcg
       61 atgggctcct ggctgtttcc gtggaaggcg gcattttcaa gtaggttatt gatgacctgt
      121 tcaatgagct ttgcatccat gggaaccata ataaaggcat cgggaacact tacatgaacc
      181 acagccaagg gaaagcgctt gcgaaatcgc tggactgcct cagaaatgac ttcctccaag
      241 ggctcttcag tctttaccac agcaggtgca ccgtccgtat cctgaatccg ggtaacggac
      301 aggagatttt cgaccatatt taagagccat tgagcatcct cttcaatatt tgaaatcagt
      361 tgacgctgtt cctccgctgt catggtattt tgctgttcga gacaggcaac gctggagcct
      421 atgattgtgg taagaggcgt tctcagatcg tgggacacag ccctcagaag gttagccctc
      481 atggtttctt tttctgcatt catcagtttt ttgtcctgct cacggatcag atttgtctgg
      541 cgggaaatca tgatgcagat gctgctggta aaacaggaga taaatgccat cccgaaaaag
      601 gtaaggggat agccgctgag agtaaaattc agcttcatgt agggcacaga gaaggcataa
      661 tttacccaaa agacactgta cagggaagcg ataatgcctg cgccgtagca gctggtagcg
      721 cgggcatgaa ggatgatagc aaggatatag acaatggata cattgatcag attgtctgtg
      781 aaatgaaaca tcaggctgga aaccagagtt gctgctgcca tataaatgat ggtggtagga
      841 agggtccgta cggtttccac aacagactgt ttcaggtgaa atggttctct ggggagaggg
      901 gaatgggagt gaagcatggt cggaagtcct tatctcagtg tagtaaagtt tacaggctat
      961 ctgcggcggt tttttcggaa cgagcgccct tttgtccgcc tgcgggggcg gtaggatggt
     1021 atagagcttc tctttttgga cagcatctga tcaaaatccg aggaggagaa gcccatatct
     1081 tctaaccgtt ctaagcgtct ctgccggcgg cgcatcagat cctcctgttc tttctgcctg
     1141 ttatgaagaa agaggaagat tcctgcgccg agaataacag caaaaccgcc ggcaagagag
     1201 actgtcagcc acaggggaag gccaaaaagt gtgcctttgt cttccgtacc tgcagcatcc
     1261 ggctgtgcag agcgcctttg gatctgtagg acaggatcgg gggactctgt ttcagatgga
     1321 accgatgctt tcgatggagc cgtcgaaaga ttggctgcat cagaagactc agagagagcg
     1381 gcgtctgaag aaatgaccgt atccggcgca cttatccggt taacctgagc cgttgtgcac
     1441 aaatatacct gtcctgtttc atgaccctca taggtatagg aaatgcgggc aatggcatgt
     1501 tttggagcat cctggccgag ttcgtaggac agactgggag tcagatcgga aaatacggct
     1561 tccctgggaa ggattacccg gctgtctgga tcaagggaca gggaaatgct gtcctttgcg
     1621 gtcattcctg caatggtcat gtcattttcc agagagcggt aggtggtttc gtggtctgcg
     1681 gcctttaagg actgaaaatt ggaaaaacca aagtcgaaaa gagcttttgt atcccggtaa
     1741 tgagactgat ggccgttcag aacaacagca atcagagtaa taccgtcacg ttcggcacag
     1801 gttaccaggg tatttcctgc cagggaggtg tatcctgttt ttccggcaaa ggctcccgga
     1861 taggtaacag atgtattttg ttttaccatg ttatggtggt tggcaacata gccgccctca
     1921 gcattttttt tggtaggcgc catctggtag ctgcgtgtgc cactgatttc cacaaaatca
     1981 ggatttttga tggcttcctg tgtgatcagc gccatgtcgt gagctgtggt gtagtgattt
     2041 tcgtcattta agccgctggg attggcaaaa ttacttcctg tacagcccag gctcctggct
     2101 ttttgattca tgagctctgc aaaagcttct ctggaaccgc ttacatgaca ggccagagca
     2161 ttggcgctct cattggcaga ggctaaaagc agagcataga ggcagtcgcg gacggtcagc
     2221 acatcgccct cgtcaattcc tgcggagctg cttcctgcct ctacattgta cacgtcatca
     2281 tgggagaaag taaccatttc atccagctca cattcctcca gtacgatcag cgctgtgagg
     2341 attttggtga tactggccgg ataataggcc tgattttcat tttttccata tagaatgctt
     2401 ccggtgtcag catccatcag gataccgccg tctgcttcaa tggaaacgcc ggtgggccag
     2461 ggaacggctg ccgcagctgt aataggcgta aagctcagaa tcaggctaag gcatataaaa
     2521 aagggaaaaa gtcgtttcat acgttacact cctatgggcg gtgcttgtgt ttcagagtta
     2581 aagccagaca gactgcaaac aggataagag acaggctgcc aaaggtaaat gcaatccgtc
     2641 cggctgtgtc cataaaaaag ccttcaggca caatattgat cagcatatcg gtggacggat
     2701 ccaggatcca cagatcgttg ctaaagaaaa tatggtggaa cataataaaa tattttgtaa
     2761 aatcggtgga gatgatggca gccagggccg cgatcagacc gaaaaacagc ccggttccca
     2821 ggcagacgga aggaggaaga aggcgtttca tatcagcctt tgtaactgcc ataaagacaa
     2881 gggcagaggc acacagaacc agacatactc tccggatgga gatggctgcc agaaacagtc
     2941 cctgaacatc ctccatatgg gcgatttctc tggcattgaa aaactcccgc tcctgtccgt
     3001 ccatgacggt catcacatga agatcttcac ggtctccccg cagataatcc atcatctcat
     3061 ctgttacttc taacagatct tccatggtca tggcaggaag atccttaagt acctcatatt
     3121 ttgtgtattc tttttcaaaa tatccgggtg tccagtatac gacagcttct atggaggtga
     3181 taaacaggac gatcatcagg cagaatgcca tcagaatgcc ggtcaggcgg tgaagatatt
     3241 tcatatcggg aagtcctcct ttgggatggg tatggttttg attcacataa aagaaaaagc
     3301 ccgacttcgc agcttgctga cgatctgcag agtccgacct ttaaacgcgc ctttataaat
     3361 cactaagggc tgcatacgtt attaaaaaga cgcataaact acctatttta tatcatgtaa
     3421 taggagagtt gtcaatgttg aaactttatg aaaaaagcca tatcaaacat ttgccgggat
     3481 acgggcgggt ttggtatggc ttttgtttgg atgtaaaagg atttaatgaa gctcaggcca
     3541 gaattctttg ttggcttcga tcatgtcatc taggatcttt ttggcaacca gaaggctggg
     3601 gacagttttt gagagggtaa atgccatcag tgctttttcg taggaatgct cgatggctgc
     3661 ctcaactacc agcttttcaa ctgcttcctg ctgctcgatc agacccttat agaaggtagg
     3721 aatctcacct acacgcacag gttcagctcc gcggtctgta atataggcag gaatttcaac
     3781 catggcatcg tcaggcagat tctcaatcgc ccccctgttt tctaccatta caaggtagcg
     3841 cttgcgcagg tcgaaggcca ggctcattgc tacgtctacg ataaattcgc cgtgtacgcc
     3901 gccaaagaat ttggtcagat cgacttcttt tcctgcgcgg taatctgcta ctgcatcaaa
     3961 aatccttttt tcgcggcctt ccattacctc gttggcgcgg gtgtggttgg gattgctgct
     4021 ttcaaccact tcatcgccca gaagataata ctgcatatag gtatttggca gataatcatg
     4081 gaatgcagag cagatatgtc tggaattggc atatgtatga agccaggagg gatcactgtg
     4141 cattacatcg ctcttttcat ttttgggcat atatccgttt tctgccacat aggactttaa
     4201 ttcctctgtt acgtcggttc cattgaggcg cactttggtg aaccatccga aatggttcag
     4261 gccgaaatag tctacttcca gatcatggcg gtcacagccc agaatctcag ccatgtttct
     4321 ctcgattgcc acaggcatat cgcagatatt caggatgcgg gcatgaggac gcaggcggaa
     4381 ggtggctttt gccacaatcg cagccggatt agagtagtta acgatccagt aagtaggctt
     4441 tgcatacttt tcgcagtagt caatcagttc taccataggg taaatggtac gcaggccgta
     4501 agcaagaccg ccggggccgc aggtttcctg gcctactacg ccgtatctta acggaatctt
     4561 ctcatcctgt tcgcgcatgg cgtacagtcc tacacgcatc tgtgcaaaga taaaatctgc
     4621 atctgtcatg gcggtttccg ggtctgtggt aagagtcaat tttacatccg gatcaaacat
     4681 ctctactaca tggcggacga taacgcccac gtcttcctga cgtttggcat tgttatcata
     4741 gagaaccagt tcattgagtt taaaacggtc tttctgtacc agaagacttt tgacgattcc
     4801 cggagtgtag gtgctgccgc ctccggcgat gacaagttta aatgttttca ttatatacct
     4861 cctgagtgat tacaagtgag ttattcttca cgtccaaggg ctttttcgac gatggaacgt
     4921 tttttgctga caacagggcc atagataatt tgtatattat ttccttttac aacaagacca
     4981 gctgctcctg ttggggcaaa ccattcttta tcctttacaa cagaggcgtc ttttacctct
     5041 acgcgcagac gggtcatgca gtttccgata ttttcaatat tgtcaggtcc gcccagtccc
     5101 tcgatgatgc gggctgcggt ttcagcatca gcttctttct ttgcttctgc tttatttccg
     5161 gagcggatct gggattttac tgctgctgca ttggcagcca ggtcgattac atcctcgccg
     5221 tcttcgcgtc ccggtgtttt caggttgaat ttcacgatca ggaaacggaa gaggaaatac
     5281 attacaacga tctcaatcag acctgcaact acatacatag gccagtgtgt ccgtccgatt
     5341 cctgccggaa ggttcagtac aaggaagtcg ataatgccgt tggtagcaca gacacggaca
     5401 tttaacagat atacgatcat ctggaacagt ccgtccatga cagagtagat gacccacagc
     5461 atgggagctg caaacaggaa ggtaaactcc agtggctctg ttacgcctac taaagaggca
     5521 gtaagtgtgg ccgggataac ctgagccttt gtggcggcct ttttctcttt tttggcagta
     5581 gcgatgaaag ccagagcaac accgatgacg ccaaaggtct tcataagtcc ataggttaag
     5641 aatcgggcag agtcgggcat ggaagcaaca gcagggtcgc tgagaagagc caggaaaaca
     5701 ggctttgcgc ctacgatctg ctctccgcca atgatcatgc tgtctccgat tgcggaatag
     5761 aggaagggag accataccag atgatggagg ccggtaggga tcagaatacg gtttaaaaat
     5821 ccatacagga atactccaaa tgcgccagcg cctgccataa tgcctgtgag ggcattgatt
     5881 gctgcagctg caacaggcca gatataggca gctgccagtg ccagcactaa tacaatggga
     5941 agaagcacga taaatacgaa cttgctgttt ccatacatag ccatggcacc cttaaattca
     6001 gtgtcaataa agcggttgtg gactaaggct acgattacgc ccaggatcat gcccaggaat
     6061 acgcccatgt ctgttacatg gtagccaaga cagatcgtct ggcctgtacc gtagagtgcg
     6121 ctggcagtat caccttccgc catcagtccc gcaacactga gccagcgaga gttggctgca
     6181 agccagatca gataactgac aagtgatacg aatgcggcat ccgctttttt cttttttgca
     6241 agactcattg ggacgcctac acagaacagg attccaagat tgccgattac tgcgctgact
     6301 ccgccattga taaattttcc agctgtatat aagatacccc cctctgatac aaatgctgtg
     6361 ttgcacatta cagaagacag agcctggagc ataccggcaa taggtaagaa tagaacaggg
     6421 cccatcaaag ctttggaaaa gctctgcatg gcgttcataa cttttgcttt catgattcat
     6481 tctccttttt cattcgtttt ctgtcggaag gcgccattcc gtttcgatgt ttgaattata
     6541 tcattttgaa taaaaaaatc tactattttg tgatagtttt aaacatattc acatagcttg
     6601 aaacatgttt cattaatgaa atcggatgta atgagaattg gttgcgcaga tggacagagt
     6661 atcgtataat ttcagtgaaa attacacaaa atatgggttt gggataaaat agagggaatg
     6721 agagatgaaa cacgatattt ataagctggc agaacaatat catttaaatg aaagtgaaca
     6781 gcagatgttt cagtcgcttt tagaggcggc agaccagaat atacagtaca gcgtaaggga
     6841 gtttgcagca cggaattacg tttcgccggc agcactgatc cgtctgagta aaaagatggg
     6901 atatactggt tatacggata tggtatatcg actgggattt ctggtacaga atgagataaa
     6961 taataagaat catgagtcag atattacagc ttttatcgga gagataccat ttgaaaaaat
     7021 gaatcgtttt ccggaacttc tgaaaaaaca cagacataat cccattttga ttacaggtac
     7081 cggattctgt gcccccttaa gggatttttt tgtgcgcaag cttctggtta tgggttactg
     7141 tgccattgga tcgaataatt atgaggtata tgagggaaat gccctttcgg caggaatggt
     7201 aattgctatt agcaaaagcg gcacaacaga cacgattatt aagcctgtcc gggatgcctg
     7261 cgcacatgga attgatgtgg tggcttttac gggaggagcc cattctatga ttgctgatat
     7321 ggcagaatta tcatttgtac ttttggatga taaaatatta gatgatcgga atcttacagc
     7381 taattatttt tatgcaagag ttcttattct atttgagtat ctgatggata tgacatggga
     7441 aacagaataa agtataagat aaaaacagga cgccgcactg caaatgatcg gtttatgatc
     7501 gtaattgctg tgcggagttt gttttttaga gtttatgtta atgagttaat gtgtattttc
     7561 aggatgatcc gcataaattt tccccatcat tttccaggca atcactgcgg ctataagctc
     7621 tgctatgggg aaggagatcc agatgcctgc gggtcccata aagcgtgaga ggatccatcc
     7681 cagaggaaca atgattacaa gctggcgcag aagggagatg atcagggaag ggcgtccctg
     7741 tcccagagcc tcaaaaactc cggctgctat gaaagccaca gaggaaatca aaaaactggg
     7801 gccgatcatg cgcagggctt ctgtcccata ctgcatcagc tccggatctg catcaaaaat
     7861 acgcagaatc gggccgggaa agaaaat
//
LOCUS       DBMDUH010000127         7662 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_4195_length_7662_cov_7.034387, whole genome shotgun sequence.
ACCESSION   DBMDUH010000127 DBMDUH010000000
VERSION     DBMDUH010000127.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 7662)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7662)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7662
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4195_length_7662_cov_7.034387"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="ACZPCG_10265"
     CDS             <1..327
                     /locus_tag="ACZPCG_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005428653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIR1285476.1"
                     /translation="ELKHGTISLIEDGTLVIGVLTQSQLYEKTLSNMAECKSRGAYLM
                     GLTSYGKYDIEDAVNFAVYVPRTDEHFSASLAVIPLQLLGYYVSVAKGLDVDKPRNLA
                     KSVTVE"
     gene            593..925
                     /locus_tag="ACZPCG_10270"
     CDS             593..925
                     /locus_tag="ACZPCG_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285477.1"
                     /translation="MQNEYLARDLSTNGCFPKAWQRSDHRFRLLKDGGEDAVNREILA
                     SSISDNITSKAFSIASAEAFNIYVLNHDRDLKKCILSLDSHNYYMMNIMDYLTGNTSC
                     EASQADGF"
     gene            897..1133
                     /locus_tag="ACZPCG_10275"
     CDS             897..1133
                     /locus_tag="ACZPCG_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285478.1"
                     /translation="MKLHKLMDFNRAFYSYDTLDGANCQTVFGEKMTQKEAAVQAVKK
                     IGLNQISEIPKGIFGLLPEYKGMFERRYGLLREL"
     gene            complement(1213..3483)
                     /locus_tag="ACZPCG_10280"
     CDS             complement(1213..3483)
                     /locus_tag="ACZPCG_10280"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HIR1285479.1"
                     /translation="MTKKELETALCTACPKAIADCTDKELYTALLAISQQAAAKKEEN
                     AGKKKLYYISAEFLIGKLLSNNLINLGLYDDVKAILEENGKSLAAVEEAEPEPSLGNG
                     GLGRLAACFLDSIATLGLNGDGIGLNYHFGLFKQLFKDNKQEEAPNPWIENTSWLTKT
                     DVTYQVPYKNFTLTSRMYDIAVTGYHGRTNHLHLFDVESVDESIVEDGISFDKTDVAK
                     NLTLFLYPDDSDREGRILRIYQQYFMVSNAARLILEEAAAKGSDFYDLPDYAVIQIND
                     THPTMVIPELIRLLTTEHGMEMDDAIEIVSRTCAYTNHTILAEALETWPMEFFKEAVP
                     QLIPIMEVLDDKVRRKFDDPSVYIIDKDDRVHMAHIDIHYGFSVNGVAALHTDILKET
                     ELNHFYKIYPEKFNNKTNGITFRRWLLHCNPQLSDFITSFIGEGYKRDADELKKLDQP
                     AISCNLKHLYRLLSIKDQKKAELAQYLKETQGLEINPNSIFDIQIKRLHEYKRQQLNA
                     LYLIHKYLEIKKGHKPTTPITAIFGAKAAPAYVIAKDIIHLILCLQQIIKEDPEVSPY
                     LNVVMVNNYNVTLAEKLIPASDISEQISLASKEASGTGNMKFMLNGAVTLGTDDGANV
                     EIHELVGDSNIYIFGDSSDTVIRRYKRGDYHSRSYYENSPALKEAVDFIISKKMLAVG
                     DKENLERLYNELLNKDWFMTFPDFAAYCRTKEKAFSDYEDRASWAKKMLVNISRAGYF
                     SSDRTIEEYNRDIWKL"
     gene            3616..4494
                     /locus_tag="ACZPCG_10285"
     CDS             3616..4494
                     /locus_tag="ACZPCG_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007716974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIR1285480.1"
                     /translation="MEKGDMIRQEKYHETKKHGLREFPFNIYPCSIPGDFQNVALHWH
                     EEMEIISIKKGRGNVVVDRVVYEVEEGDVMAVFPGQLHAIYGFEDQRMDYENIIFSLS
                     LLMGAEEDLCTERFLFPLAREQVREPLFKRRGMEGYEAFAGCIQALDCVSEERGTGYQ
                     LAVKGQLYTFLYLAFQSGLTLWEKRPGRARERIKQVLGYIEEHYGEEFDISQAAGMCF
                     YSQSHFMKYFKQYTGQPFVSYLNDYRLSRAGGFLRTTGDTVTEIAVRCGFDNLSYFNR
                     LFRRKYGMTPKEYRES"
     gene            4572..6122
                     /gene="malQ"
                     /locus_tag="ACZPCG_10290"
     CDS             4572..6122
                     /gene="malQ"
                     /locus_tag="ACZPCG_10290"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HIR1285481.1"
                     /translation="MRECGILLPVASLPSRYGIGAFSKEAYEWIDRLKQAGQGFWQVL
                     PFGPTGYGDSPYQSFSAFAGNPYFIDLEQLIREGLLTKEECDKADFGDSRRYISYDRI
                     YRERFPLLRKAYDRWKDGLARKGTAGKESAENTCGVLEQVHGLALETLGPETREYCFY
                     MALKDHFQSKSWNLWERGIRLREPEALKAYGSLLTDEIGFYEFQQMKFEEQWTALKNY
                     ANKQGIRIIGDIPIYVAFDGADSWCHPELFQFDEENLPKAVAGCPPDAFAETGQLWGN
                     PLYDWGYHEKTGYEWWIRRMEYSLRMYDVVRVDHFRGFEAYYSIPYGDATAEFGHWEK
                     GPGMALFQALEAHFGDELPVIAEDLGFLTPEVYELLEKTGFPGMKVLEFAFDDSEESG
                     YLPHNYTENCVVYTGTHDNDTLTGWFDSMSKKNRSFAREYTGSRRVPKKELHWQFIRL
                     ALESPARLAVIPIQDYLGLGTEARINEPSTLGKNWKWRLLPGEVDQALIEKCRRLAKI
                     YGRLAKNS"
     gene            6219..7649
                     /locus_tag="ACZPCG_10295"
     CDS             6219..7649
                     /locus_tag="ACZPCG_10295"
                     /inference="COORDINATES: protein motif:HMM:NF039231.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HIR1285482.1"
                     /translation="MKKETNVKRTGCYIRRNRFILGAAAGLLLAAALPFGALGAEKGW
                     QMVDGAERYVDETGGIVTEQWKKRDGKSYYLDENGEIAADAWIQGTWHVDETGAMEKE
                     TWIYEDGSSQMAKGWYYLGPSGKAEKNDWKKLGDVRYSFDSQGRMRTGWHYENGDIYY
                     LGDENQGYTQIGWRYLESDGKKGPAEGSVSKALAPGEPGGQWYYFNSSGKARRAEVGS
                     YKESEIDGHRYYFDANGVMATGWRCVREQAKPGDGTGISRFVYLGGKDEGMLKSQWLE
                     TEERPWDSPEWEQALSKEARRTKNASETAQTKEDMTRRFYLQHDGTPAFLSADAVQIW
                     DAVTKLDGAYYFFDSYGVQQSGLIRMTSGGKTETAWFDPDKDGVLLTGKAEAVKDKNG
                     KTFAFCAETSGSLKGTGVNGEKDGFLYYNGLLAAAPEGTGYASFKIGDRIWTADEKGR
                     IQTEDKMRKAETAPLPAAGWEISYVR"
BASE COUNT         1970 a         1562 c         2187 g         1943 t
ORIGIN      
        1 gagttaaagc acggaaccat aagcctgatc gaggacggta cgctggtgat cggagtgctc
       61 acacagagcc agctttatga aaaaacgctg tccaatatgg ctgagtgcaa aagccggggg
      121 gcttatctga tgggcttgac ttcttatgga aaatatgata tagaggatgc ggtaaatttt
      181 gcggtatatg tgcccagaac cgatgaacat ttttctgcca gtctggcagt gatccctctt
      241 cagctgcttg gatattacgt tagtgtggca aaaggactgg atgtagataa gccgagaaat
      301 ctggcaaaat ctgttacggt agagtaaagg gcgcagatcc gattacaaag gagattgaga
      361 gaagctggga gtgattgcgg ctgccatgga atttgtttgt aaaatacaac cgatgccaga
      421 atgacgatta tccgtagaaa gagccgcatt gtcgtaccgc tgtgcgtctt taacagatgt
      481 atattgggtc agatataagg gcgagagggt taaatttgat gagatcaatt tatatgataa
      541 tcatctcagc aataccttta tggatattac attgagagga agacaatata ctgtgcagaa
      601 tgaatatctg gcaagagatc tttctaccaa tggttgtttt cctaaggcat ggcagcggtc
      661 tgaccatagg ttcaggttgc tgaaagacgg aggagaggat gcggtaaaca gggaaattct
      721 tgccagcagt atcagcgaca atattacatc aaaggcattt tctattgcat cggcagaggc
      781 atttaatata tatgtcttaa atcatgatcg tgatttaaag aaatgtattt tgtcattaga
      841 ctcccacaat tattacatga tgaatataat ggattatttg acagggaata cgtcctgtga
      901 agcttcacaa gctgatggat tttaaccgtg cattttattc atatgatact ttggatggag
      961 caaactgtca gactgtattt ggggaaaaaa tgacgcagaa agaggctgca gttcaggcag
     1021 taaagaagat tgggctgaat cagatcagcg aaatcccgaa aggtattttt gggctgctgc
     1081 cggagtataa aggtatgttt gaaaggcggt atgggcttct gcgggaatta tgaggtgtat
     1141 ggagattagt tttgccccct cccaaccggc tccgaacgaa tcggaacccc ggctgtgagg
     1201 gggcgttttt atttacagct tccagatatc cctgttatac tcctcaatgg tccggtctga
     1261 ggagaagtat cctgccctgc tgatatttac cagcattttc ttcgcccagg aggcccggtc
     1321 ctcatagtcc gagaaggcct tttccttggt acggcagtag gcggcaaaat cagggaaggt
     1381 catgaaccag tccttattta aaagctcatt gtaaaggcgt tccagatttt ccttatcgcc
     1441 cacagcaagc attttcttac tgatgataaa gtctacggcc tcctttaagg caggggagtt
     1501 ttcgtagtag gatctggaat ggtagtcccc tctcttatag cggcggatga ccgtatcgct
     1561 ggagtcgccg aagatataga tgttggagtc gcctaccagc tcgtggatct ccacattggc
     1621 tccgtcatcc gttcccaggg tgacggctcc gttcagcatg aatttcatat ttcccgtgcc
     1681 gctggcttcc ttggaggcca gcgagatctg ttcggagata tcgctggccg ggatcagctt
     1741 ttctgccaga gtcacgttgt aattatttac cattactaca tttaagtagg ggcttacctc
     1801 aggatcctcc tttatgatct gctgcaggca gaggatcaga tggataatgt ctttggcgat
     1861 cacataggca ggcgcagcct ttgcaccgaa gatggccgtg atgggagtag tgggcttatg
     1921 gccttttttg atctccagat atttgtggat caggtacagg gcgtttaact gctggcgctt
     1981 gtactcgtga aggcgcttga tctggatgtc aaagatggaa ttgggattga tttccagtcc
     2041 ctgtgtttcc tttaaatact gtgccagctc tgcttttttc tgatccttaa tggaaagcag
     2101 gcggtacagg tgctttaaat tgcaggaaat ggcaggctga tccagttttt tcagttcatc
     2161 tgcatccctt ttatagcctt ccccgataaa gctggtgata aaatcagaga gctgtggatt
     2221 gcagtggagg agccagcggc ggaaggtgat cccattggtt ttgttattaa atttttcagg
     2281 atagattttg taaaaatggt tcagctccgt ttcctttaaa atatcggtgt gcagagcggc
     2341 aactccgttt acgctgaagc cgtagtggat atcaatgtgg gccatgtgaa cccggtcgtc
     2401 cttatcaatg atatagacgg aaggatcgtc aaatttacgg cgtaccttgt catccagcac
     2461 ctccataatg gggatcagct ggggcaccgc ctccttgaaa aattccatgg gccaggtttc
     2521 caaggcctca gcaaggatag tgtggttggt gtaggcgcag gtgcggctga cgatttcaat
     2581 ggcgtcgtcc atttccatgc catgctctgt ggtcagcagg cggatcagct ccgggattac
     2641 catagtagga tgggtgtcat tgatctggat gacagcatag tcgggcagat catagaaatc
     2701 agagcctttt gcagcagcct cttccaggat cagccttgcg gcgttgctta ccatgaaata
     2761 ctgctggtag atgcgcagga tccgtccctc cctgtcgctg tcatcggggt agaggaacag
     2821 agtcaggttt tttgccacat ccgttttgtc aaaggaaata ccgtcctcta caatggattc
     2881 gtccacggat tccacatcaa ataaatggag atgattggta cggccgtgat agcctgttac
     2941 cgcgatatcg tacatgcggg aggtaagggt gaagttttta tagggaacct gataggtcac
     3001 atcggttttt gtaagccagg aggtgttttc aatccaggga ttgggagcct cctcttgttt
     3061 attatcctta aagagctgct taaaaaggcc gaagtgatag ttcagcccga tgccgtcgcc
     3121 gtttaagcca agagtggcaa tggagtccag aaagcaggct gccagacgtc ccaggccgcc
     3181 gtttcctaag gatggctctg gctctgcttc ctcgactgct gccagagatt tgccattttc
     3241 ttccagaatc gcctttacat catcataaag gcccagattg atcaggttgt tggaaagaag
     3301 ctttccgatg agaaattcag cagagatata gtacagcttt ttctttccgg cgttttcttc
     3361 cttttttgca gcggcctgct gggaaatggc aagaagggct gtatagagtt ctttatccgt
     3421 acagtcggcg atcgctttag gacaggcggt gcacagtgct gtttcaagtt ctttttttgt
     3481 catatcagat ccatcctttc tgctcggtgc aggagttatt tatattacag ttattctatc
     3541 aggtggcttg gaaaaaatct tgcagaatga tgggagaata tagtacaata cgatcaaaag
     3601 gaggtggaag gcagcatgga aaaaggcgat atgataaggc aggaaaagta ccatgaaacc
     3661 aagaagcacg gactgcgcga attccctttt aatatctatc cctgttccat tccgggggat
     3721 tttcagaatg tggctctcca ctggcatgag gaaatggaga tcatttccat aaaaaagggt
     3781 cggggcaacg tggttgtaga ccgggtggtt tatgaggtgg aggaagggga tgtgatggct
     3841 gtctttcccg gccagcttca tgcaatttac ggatttgagg atcagagaat ggattacgaa
     3901 aatatcattt tcagcctgtc acttctcatg ggggcagagg aggacctctg tacggaacgg
     3961 ttcctttttc ctcttgccag agagcaggtg agggagccgc tttttaaacg gagaggaatg
     4021 gagggatatg aggcgtttgc aggatgcatt caggcgctgg actgtgtatc ggaggagagg
     4081 ggaaccgggt atcagctggc agtgaagggg cagctttata cctttcttta tctggccttt
     4141 cagtccggcc tgacgctgtg ggaaaaacgg cccggacggg ccagggagcg gattaagcag
     4201 gtgctgggat acattgagga gcattacgga gaggagtttg atatcagcca ggccgccggg
     4261 atgtgctttt acagccagtc tcattttatg aaatatttta agcagtatac gggacagccc
     4321 tttgtgagct atctgaacga ttaccgtctt tcccgtgcag gcggattttt aagaaccacg
     4381 ggagacacgg tgacggagat tgcagtccgg tgcggttttg ataatctgtc ctattttaac
     4441 cgtctgttcc gcagaaagta cggtatgacc ccgaaggagt atcgggagtc ttaaggccgg
     4501 cagtccaaag cgattgattt tgtctccgtt tttccgtata atgatgtgga ttgcaaaaag
     4561 ggggttattt tatgcgcgaa tgtggaattt tgcttcctgt ggcaagcctg ccctcacggt
     4621 atggcatcgg agccttttca aaggaagcct atgaatggat cgaccgttta aagcaggcgg
     4681 gacagggctt ttggcaggtg cttccctttg gccctacggg ctatggggat tcgccctacc
     4741 agtccttttc cgcttttgcg ggaaatccgt attttatcga cctggagcag ctgatccggg
     4801 agggcctttt gacaaaggaa gaatgcgata aggctgattt tggggattcc aggcggtata
     4861 tttcctatga ccggatctac agggagcggt ttccgctgct tagaaaggcc tatgatcggt
     4921 ggaaggatgg gctggcccgg aaggggactg cggggaaaga atctgcagag aatacgtgcg
     4981 gcgttctgga gcaggttcac ggtctggccc ttgaaactct tgggccggaa acaagggaat
     5041 actgctttta tatggctttg aaggatcatt tccagtcaaa aagctggaat ctctgggagc
     5101 gcgggatccg tcttagagag ccggaggcgc tgaaagccta cggcagcctt ctcaccgatg
     5161 agatcgggtt ttatgagttt cagcagatga aatttgaaga gcagtggaca gcgttaaaaa
     5221 attatgccaa taaacagggg atccggatca tcggagatat tcccatttat gtagcttttg
     5281 acggcgcgga cagctggtgc catccggagc tgtttcagtt tgatgaggaa aatctgccaa
     5341 aggcagtagc gggctgtccg ccggatgcct ttgcagagac ggggcagctt tggggaaatc
     5401 ctctttatga ttggggatat catgagaaaa ccggctatga atggtggatc cggcggatgg
     5461 aatacagcct tcggatgtat gacgtagtgc gggtcgacca tttcagaggc tttgaagcct
     5521 attattccat tccctacggc gatgcgacag cagagttcgg acactgggaa aaggggccgg
     5581 gaatggcgct gtttcaggcg ctggaggcgc actttgggga tgagcttccg gtgatcgcag
     5641 aggatctggg ctttcttacc cctgaggtct atgagctttt ggagaaaaca ggatttccgg
     5701 ggatgaaggt tttggagttt gcctttgacg attcagagga aagcggctat cttcctcata
     5761 attatacgga aaactgtgtg gtttatacgg gaacccatga caatgatacg ctcacaggct
     5821 ggtttgattc catgagcaaa aagaaccgca gctttgccag agagtatacg ggaagccgcc
     5881 gggttccaaa gaaggagctt cactggcagt ttatccgtct ggccctggaa agtccggcca
     5941 gactggctgt gatccccatt caggattatc tgggccttgg cacagaagcc cggatcaatg
     6001 aaccttccac attagggaaa aactggaaat ggaggctgct tccgggagaa gtagatcagg
     6061 ctttgattga aaaatgccgc aggctagcaa agatttacgg cagactggca aaaaacagtt
     6121 aagttaataa aatcttctga ttataatcgg taaaaattca tggattttgt gtcattttct
     6181 gataaaataa agataagaat aatcaggagg tggcggctat gaagaaggaa acgaatgtaa
     6241 aaagaacagg atgctatatc agacggaatc gctttatttt aggagcagca gcgggccttc
     6301 ttctggcagc tgcccttccc tttggagcgt tgggagcaga aaaaggctgg cagatggtgg
     6361 acggagcaga gcgctatgtg gatgagaccg gcggtatcgt tacggagcag tggaaaaaac
     6421 gggacggcaa atcctattat ctggatgaaa acggtgagat tgcagcagac gcctggatac
     6481 agggaacctg gcacgtagat gaaacaggag ccatggaaaa ggaaacatgg atctacgagg
     6541 atggttcctc tcagatggcg aaaggctggt actatctggg acccagcggc aaggcggaaa
     6601 agaacgactg gaaaaagctg ggagatgtcc ggtacagctt tgacagccag ggacggatgc
     6661 gcacaggttg gcattatgaa aatggagata tctattatct gggagatgaa aaccagggat
     6721 atacccagat cggctggcgt tatctggaaa gcgacgggaa gaaggggccg gcagaaggct
     6781 ctgtttccaa agcgctggct cccggagaac ccggaggaca gtggtattat tttaactctt
     6841 cgggaaaggc cagacgggca gaggtgggct cctataagga aagcgagatt gacggccatc
     6901 gttactattt tgatgccaac ggcgtgatgg cgaccggatg gcgctgcgta agggaacagg
     6961 caaagcccgg agacggcaca ggaatcagcc gctttgtgta tctgggcgga aaagacgaag
     7021 ggatgcttaa gagccagtgg cttgaaacag aggaacggcc ctgggacagc ccggaatggg
     7081 agcaggcctt atcaaaagaa gcccgccgta ctaaaaatgc ttccgagacg gcacagacaa
     7141 aggaagatat gacacgtcgt ttctatctgc agcatgacgg tactcctgct tttttatcag
     7201 cagatgccgt gcagatctgg gatgcagtga caaagctgga tggagcctat tatttctttg
     7261 attcctacgg ggtacagcag agcgggctga tccgcatgac ctcaggaggg aaaaccgaga
     7321 cggcctggtt tgatccggat aaggacggcg tccttttgac aggaaaagca gaagccgtaa
     7381 aggataaaaa cggcaaaaca tttgccttct gtgcagaaac ctcaggctcc ttaaagggaa
     7441 ccggggtaaa cggagaaaag gacggctttc tttattataa cggccttctg gcggctgccc
     7501 ctgaaggaac cggctatgcg tcctttaaga tcggagacag gatctggacg gcggatgaaa
     7561 aggggcggat ccagacagag gataagatga gaaaggcaga gacagcaccg cttccggcag
     7621 caggctggga gatatcgtat gtgagataaa gaggttgcca aa
//
LOCUS       DBMDUH010000128         7596 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_4222_length_7596_cov_4.325284, whole genome shotgun sequence.
ACCESSION   DBMDUH010000128 DBMDUH010000000
VERSION     DBMDUH010000128.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 7596)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7596)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7596
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4222_length_7596_cov_4.325284"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="ACZPCG_10300"
     CDS             complement(<1..260)
                     /locus_tag="ACZPCG_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1285483.1"
                     /translation="MKLNRTALRTVDILKLVSKKPDGITLDQICEQLSIPKTSAYDIL
                     TTLVHTGMVHVAREQKQRYTIGLTAYRVGINYTNNMDFIGTI"
     gene            complement(307..1791)
                     /locus_tag="ACZPCG_10305"
     CDS             complement(307..1791)
                     /locus_tag="ACZPCG_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HIR1285484.1"
                     /translation="MQDIVKNNQNDNVAVALRPLNKGEVLQAAETAVTLMEDIPQGHK
                     FALREIKSGEEVVKYGFRIGFAKEDIQPGQWVHVHNVKTALGDVLTYDYEPEGHQDVE
                     PTEHTYFEGYRRTDGKAGIRNEIWIIPTVGCVNSIAKALETAAKKFVGGNVEDVIAFQ
                     HPYGCSQMGDDQENTRKVLADMIHHPNAGGVLVLGLGCENSNIPVLMDYIGAYDEQRV
                     KFLQCQDVEDEMEEAMKLIGELAAYAGAFSREKIDASELIIGMKCGGSDGLSGITANP
                     VVGAFSDLLVSKGGTTILTEVPEMFGAETLLMNRCATPELFDKTVDLINNFKNYFTSH
                     NQTIYENPSPGNKKGGISTLEDKSLGCTQKSGSALVKGVLEYGEPVKVKGLNLLSAPG
                     NDLVAATALAVSGAHMVLFTTGRGTPFASPVPTVKISSNSKLAGHKNNWIDFNAGRMV
                     EDKTKEELAEELLGYVLEVASGRKVKSEEAGFHDMAIFKQGVTL"
     gene            2004..3005
                     /locus_tag="ACZPCG_10310"
     CDS             2004..3005
                     /locus_tag="ACZPCG_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR1285485.1"
                     /translation="MNIYDVSKHAGVSIATVSRVLNGNPNVSERTREKVLRVMDELGY
                     TPNVFARGLGLDTMQTIGIMCSDSSDPYLAGAIYYLERELRSHNYDSILCCTGYDLKV
                     KQKYFNLLRSKRVDAIILAGSKFVELCPKDNDYILKAAHDIPVMLVNGYLEGENIYST
                     VCDDYSAVYDAVSRLIRSGSRRILYLYTSYSYSGLNKMDGYKAALTSCGLPVREDLIH
                     QCSKNLEDARDLLLRLSKQGLDFDAVVTSDDSLAVGAVKYAHAKGISVPDQLSIIGYN
                     NSLLSRCTDPEITSIDSRVETLCTTTVKTLMGVFSGESVPSRTTIASDLIKRNTTNF"
     gene            3028..4443
                     /gene="uxaC"
                     /locus_tag="ACZPCG_10315"
     CDS             3028..4443
                     /gene="uxaC"
                     /locus_tag="ACZPCG_10315"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002593418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HIR1285486.1"
                     /translation="MKQFLDKDFLLSTEMAQKLYHDYAETMPILDYHCHINPQEICED
                     RKFENITQVWLGGDHYKWRQMRSNGVDEKYITGDATDREKFQAWAETLPKLIGNPLYH
                     WSHLELRRYFGYEGYLNGDTAEEVWNLCNAKLQEDSMTVRNLIRQSNVTLICTTDDPV
                     DTLEYHKKLAEDTSFEVQVLPAWRPDKAMNVEKPTYAAYIKQLSEVSGVEIKDFASLK
                     EALKNRMEFFNSMKCCVSDHALEYVMYAPASDAEVDAIIAKGLKGEAVSREEELKYKT
                     AFMLFVAREYNRMGWIMQLHYGCKRDNNAYMFEQLGPDTGFDCINNYAPSAQMADFLN
                     ALSATNEIPKTIIYSLNPNDNASIGTILGCFQEKFPGKIQQGSAWWFNDHKTGMTEQM
                     TSLGNLGCLGNFIGMLTDSRSFLSYTRHEYFRRIMCELIGGWVDNGEYPADMKSLETI
                     VKGICYNNAVNYFGFDLKTVE"
     assembly_gap    4462..4471
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4528..5166)
                     /locus_tag="ACZPCG_10320"
     CDS             complement(4528..5166)
                     /locus_tag="ACZPCG_10320"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1285487.1"
                     /translation="MINIRNEEKADYAIVEQITREAFYNMYVPGCVEHYLVHIMREHK
                     DFIPELDFVLELNGTVIGNIMYTKAWLLDEEGNKKEILTFGPVCIKPGYQRRGYGRKL
                     IEHSFEKAVKLGYDTVVIFGSPVNYVGCGFKSCKKYNVGTENGKYPSAMMVRELIPGA
                     LDGHKWIYQDSPVMAISEEEAQKYDDTLEKMEKKCQPSQDEFYIMSHSFIEE"
     gene            complement(5289..5948)
                     /locus_tag="ACZPCG_10325"
     CDS             complement(5289..5948)
                     /locus_tag="ACZPCG_10325"
                     /inference="COORDINATES: protein motif:HMM:NF012655.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR1285488.1"
                     /translation="MKRDHLSKGRIQRLLEGWSFLLPERMERVDKIALTSERLFTSGG
                     LTMTDTKESILKAALGLFAVKGYEAVSVSQIAGTLGMTKGALYKHYKNKRDIFDSIFT
                     YVCELDAERSRKSGVPEKEFSEMPDSFSDVSPKSLKEYMLSQFHYWSMDETACNFRKM
                     LTLEQYKTTEAGTLYEKVLTDGPLTYIEDIFREMIIHGKLIKKSPDSWQLSFMCRFIC
                     Y"
     gene            complement(5948..6303)
                     /gene="rnpB"
                     /locus_tag="ACZPCG_10330"
     ncRNA           complement(5948..6303)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACZPCG_10330"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            6572..>7596
                     /gene="yedE"
                     /locus_tag="ACZPCG_10335"
     CDS             6572..>7596
                     /gene="yedE"
                     /locus_tag="ACZPCG_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YedE family putative selenium transporter"
                     /protein_id="HIR1285489.1"
                     /translation="MKETLNSKKGLLVWGGLLGAVAVWLAIMGNPGNMAFCIACFIRD
                     IAGAAKLHTAPIVQYMRPEIAGLIAGAFIISMATGEYRSTAGSSPMIRFLLGIITMIG
                     ALVFLGCPLRMVIRMAAGDLNAYMGLIGFVLGVGTGCIFLKKGFSLGRSFDTKKENGY
                     IIPAIFLSLLVLSIATPLFAASTEGPGSLHAPILVSLAAGLIFGAAAQKARACFAGGI
                     RDVILMKNFDLLSVIAGLFGVMLVYNLVSGNFKPGFEGQPIAHAETLWNILGTYTVGF
                     AAVLAGGCPLRQLILAGQGSSDAAVNVLGMLVGAALCHNFGLASSGTGTTPAGRIMCI
                     VCIAVLFA"
BASE COUNT         1849 a         1901 c         1671 g         2165 t
ORIGIN      
        1 tctatggttc cgataaaatc catattgttg gtataattga ttcctacccg ataggcggta
       61 agccctatgg tataacgctg cttctgctct ctggccacat gaaccatccc cgtatgcacc
      121 agagttgtaa gaatatcata ggcgcttgtc tttgggatgg aaagctgctc acagatctga
      181 tccagcgtaa tgccgtccgg cttttttgac accagtttta atatatccac cgtccgcagt
      241 gccgtcctgt ttaatttcat cgtgtctcct tattatatgc agtccggact acctgcccgg
      301 ttcattttac agggtcacgc cctgtttaaa gatagccata tcatggaaac ctgcttcctc
      361 agattttacc ttgcggccgg aagcaacctc cagcacatag ccaagcagtt cctctgccag
      421 ttcttccttt gtcttatcct cgaccatacg gcctgcgtta aaatcgatcc agttattctt
      481 gtggcctgca agcttggaat tactggaaat ctttacagta ggaaccgggg atgcaaaggg
      541 tgtgccgcgg ccggttgtaa agagcaccat atgggcgccg gacacagcca aagccgtagc
      601 tgccaccagg tcattgccgg gagcacttaa aagatttaag ccctttacct taaccggttc
      661 gccgtactcc agcactccct ttacaagagc gcttccggac ttctgggtgc agcctaagga
      721 cttgtcctcc agagtggaaa taccgccctt tttgttaccg ggggatggat tttcataaat
      781 ggtctggtta tggcttgtga agtaattttt aaaattattg atcaggtcta cggtcttgtc
      841 aaaaagttcc ggagttgcac agcggttcat aagaagggtc tcagcgccga acatctcagg
      901 cacctcagtc aggatcgttg taccgccctt gcttactaaa agatcggaaa atgctcctac
      961 taccggattg gccgtgattc cggaaagtcc gtcggagccg ccgcatttca tgccgataat
     1021 cagctcgctg gcatcaattt tctctctgga aaatgctcct gcataggctg ccagctctcc
     1081 gatcagcttc atggcctcct ccatctcgtc ctctacatcc tggcactgga ggaacttcac
     1141 tctctgttcg tcatacgctc cgatatagtc cataagcaca ggaatattgc tgttttcgca
     1201 gcccagtccc agaacaagca cgccgcctgc atttggatgg tggatcatat ctgccagaac
     1261 cttacgggta ttctcctgat cgtctcccat ctgggagcag ccgtatggat gctggaatgc
     1321 gatcacgtcc tctacattac ctcccacaaa tttcttagca gccgtttcca gggcctttgc
     1381 aatggagttc acacagccta cggtaggaat gatccagatt tcattgcgga ttcctgcctt
     1441 cccgtcggtt ctgcggtagc cttcaaaata tgtgtgctct gtgggttcta catcctgatg
     1501 gccttccggc tcataatcat aagtaagaac atctccaaga gccgtcttca cattatgaac
     1561 gtgaacccac tgtcccggct ggatatcttc cttggcaaaa ccgatgcgga agccgtactt
     1621 tacaacctct tctccgctct ttatttccct cagcgcaaac ttgtggccct gggggatatc
     1681 ttccataagg gtcactgcgg tttctgccgc ctgcagtacc tctcccttat ttaaagggcg
     1741 cagtgcaacg gcaacattgt cattctgatt gttctttaca atatcctgca tgatcgcttc
     1801 atgctcctgt tctttacttg gtgctctctg gcctgacagt cttttgggga tgccggtcag
     1861 gtctatgtaa gtgcttacat tcattttatc cattttcccg taagaagtca ataaaaaaat
     1921 agaaaactct tgcaatattt tccgaaaaaa tgtataataa agttagcttg taagcgctta
     1981 cagaatgtga ggttattcca tacatgaata tatatgatgt atcaaaacat gccggagtat
     2041 ccattgccac ggtttcccgt gtattaaacg gcaatcccaa tgtcagcgag cgtacccgtg
     2101 aaaaggttct gcgcgtcatg gatgaacttg gctatacacc caatgtattt gccagaggac
     2161 tggggcttga caccatgcag accataggga tcatgtgttc cgattcctcc gatccctatc
     2221 tggccggtgc catctactat ctggaacggg aacttcgcag ccataactat gattctattc
     2281 tctgctgtac gggttatgat ctgaaggtca agcagaagta ttttaatctg ctgcgctcca
     2341 agcgtgtaga cgccattatt ctggccggtt caaaatttgt ggagctttgc cccaaggata
     2401 atgattatat cttaaaagct gcacatgata tacctgtcat gcttgtaaac ggttatctgg
     2461 agggggaaaa tatctacagc acagtctgcg atgattattc tgctgtgtat gatgcggttt
     2521 cccggctgat ccgttccgga agcaggcgga ttctttatct gtataccagc tattcttaca
     2581 gcgggttaaa caagatggat ggatataagg cagctcttac ttcctgcgga cttcccgtgc
     2641 gggaggatct gatccatcag tgttccaaaa atcttgagga tgccagagac ctgctcctga
     2701 gactgtcaaa acaggggctt gacttcgatg cagttgtaac ctcggatgat tccctggcag
     2761 taggagcggt taaatacgcc catgcaaaag ggatttccgt accagaccag ctcagtatca
     2821 tcggctataa taattctctt ttatcccgct gtacagatcc tgagatcact tctatcgaca
     2881 gccgggtgga gacactgtgt acgactacag taaaaacact tatgggagtt ttcagcggcg
     2941 aatctgtgcc cagccggact acgatcgcct ctgatcttat aaaaagaaac accacaaact
     3001 tttaacattt aaggaggctc tactaaaatg aaacagtttt tagacaagga tttcttatta
     3061 tctaccgaaa tggctcagaa gctttatcac gactatgctg agaccatgcc tatcctggac
     3121 tatcactgcc atatcaatcc ccaggaaatc tgtgaggatc gcaaatttga gaatatcact
     3181 caggtatggc tgggaggcga ccactacaaa tggcgtcaga tgcgttccaa cggcgttgat
     3241 gaaaaatata tcaccggcga cgctacggac agggagaaat tccaggcttg ggccgagacc
     3301 cttccaaagc tgatcggcaa ccccctgtac cactggagcc atcttgaatt aagaagatac
     3361 ttcggctacg agggctactt aaacggcgat accgctgaag aagtatggaa tctgtgcaac
     3421 gcaaagctgc aggaggattc catgactgta cgcaacctga tccgccagtc caatgttacc
     3481 ttaatctgca ccaccgatga tccggttgac accctggaat accacaagaa gctggctgag
     3541 gatacttcct ttgaggtaca ggttcttccc gcatggagac ctgataaggc tatgaacgtt
     3601 gaaaagccaa cctatgcagc ttatattaaa cagctctctg aggtaagcgg cgttgagatc
     3661 aaggattttg cttctttaaa agaggcctta aagaaccgta tggaattctt taattccatg
     3721 aagtgctgtg tatctgacca tgctcttgag tatgtaatgt acgctcctgc ttctgacgca
     3781 gaggtggacg ccatcatcgc aaagggctta aagggcgaag ctgtttcccg cgaagaagag
     3841 ttaaagtaca agaccgcgtt catgctgttt gttgcaagag aatacaacag aatgggctgg
     3901 atcatgcagc ttcactacgg ctgcaagcgc gacaacaacg catatatgtt tgagcagctt
     3961 ggtcctgata ccggctttga ctgtatcaac aactacgctc caagcgcaca gatggcagac
     4021 ttcttaaatg cattaagcgc taccaatgag attccaaaga ccatcatcta ctccttaaat
     4081 ccaaatgata atgcttccat cggcaccatc ctgggctgct tccaggagaa gttccccggc
     4141 aagatccagc agggttccgc atggtggttc aatgatcaca agaccggcat gaccgagcag
     4201 atgacctctc ttggaaactt aggctgcctc ggaaacttca ttggtatgct gaccgactcc
     4261 agaagcttcc tgtcctacac cagacatgag tatttccgcc gcattatgtg cgaactgatt
     4321 ggcggctggg tagacaacgg cgaatatccg gcagatatga agtccttaga gaccatcgta
     4381 aagggtatct gttacaacaa tgctgtaaat tactttggat ttgatttaaa gacggtagag
     4441 taatttacag cattgttgta annnnnnnnn nttaaacgta tattcaaaat gggaggttcc
     4501 ctttgacggg gcctcccatt ttcgatccta ttcttctata aacgaatggc tcataatata
     4561 aaattcatcc tggctgggct ggcatttctt ttccatcttt tccagagtat catcatattt
     4621 ctgtgcctct tcttcactga ttgccatcac cgggctgtcc tggtaaatcc atttgtgtcc
     4681 gtcaagagcg ccgggaatca gttcccttac catcattgca gaaggatatt ttccattttc
     4741 cgtacctaca ttgtattttt tgcagctctt aaagccgcac cccacataat tgaccgggct
     4801 tccaaatatc accactgtgt catatccaag cttaacagcc ttttcaaagg aatgttcgat
     4861 cagctttctg ccatagcccc ttctctgata accaggctta atgcagaccg ggccaaaggt
     4921 caaaatctcc tttttatttc cttcttcatc caaaagccat gcttttgtgt acatgatatt
     4981 tcctattaca gttccattta actccaggac aaagtccagc tccggtataa aatccttatg
     5041 ttcccgcata atatgcacca gataatgctc gacgcatccg ggaacataca tattgtaaaa
     5101 ggcttctctt gtgatctgct caacgattgc gtagtctgct ttctcttcat ttctgatatt
     5161 gatcatttcc tgcattcttg tttcctcctc aggcaataaa tatttgcgaa aaaaatcatc
     5221 catctgcttc tcgtagacgg cttccatctc ttctttgtct ttcttacgtc cagttctgtc
     5281 ggccatgttt agtaacaaat aaaacggcac ataaaactca actgccagct gtctgggctt
     5341 ttttttataa gcttgccatg tattatcatt tcacgaaaaa tatcttctat gtaagttagc
     5401 ggaccgtctg ttaaaacctt ttcatacaat gtgcctgctt ctgtggtttt atactgttcg
     5461 agggtcagca tttttcgaaa attgcaggca gtttcatcca tactccaata atgaaactgg
     5521 gaaagcatat actccttcag gcttttggga gatacatctg aaaaggaatc tggcatttcg
     5581 gaaaactcct tttcagggac gccgcttttt ctggagcgtt ctgcatccag ttcacagaca
     5641 tatgtaaaaa tagaatcaaa gatatctctt ttatttttgt aatgcttata taatgcgcct
     5701 tttgtcatgc ccagagtccc tgcaatctgg ctgactgaaa ctgcttcgta gccctttacg
     5761 gcaaataatc ccagcgcagc ttttaaaata ctttcttttg tgtccgtcat agtaagacct
     5821 ccagaagtaa acaatcgttc acttgtcaag gctattttat ctaccctttc catcctttca
     5881 ggcaacaaaa aagaccaccc ctccaaaagc cgctgtatgc gtcctttgct tagatgatct
     5941 ctcttcatgt gtgagcaaaa cgataagccg ggttatgtcg ttggatggcc atctatctag
     6001 aactgctgtc gccagcagtc tcaagcaacc tacccggaag ctgacgggcc gccatatgct
     6061 tcctgtttgg tcttgcttcg agtggggttt acatgtgccc cgcctgttac cagccgggcg
     6121 gtagtctctt acactgccct tccaccctta cctgcctgct ttcgcagact ggcggtatat
     6181 ttctgttgca ctgtccctgg agtcgcctcc gccagacgtt atctggcacc ctgccctgtg
     6241 aagcccggac tttcctctcc tgcggcctct cggtatcgca gcagcggcca tctgtcttac
     6301 tcacaactat agtagtttaa cacaaaaccg gcggcatgtc cagtttttga gaatttttct
     6361 tcaactcatt tttcctgaat tggaaaatta tacaaaaacc agttgacctc cccttctgtt
     6421 aatgctacaa tttaatcaga aaaaggaata cgcagttgga gcttcctaaa tgtttcagta
     6481 aaagccggct cggccaccgt cccagagtgg tcaggccgtt ttttattgcc tgcgttattc
     6541 tttttttaat ataacgtttg gagggaatga aatgaaagaa acactcaatt caaaaaaggg
     6601 gctgcttgta tggggcggcc tgctgggagc cgtggctgta tggctggcta ttatgggaaa
     6661 tcccggaaat atggcatttt gtatcgcctg ctttatccgc gacattgcag gcgcagccaa
     6721 gctgcacacg gcacccattg tacaatatat gcgccctgaa atcgcaggcc tgatagccgg
     6781 agctttcatt atttccatgg ccaccgggga ataccgctct accgccggct cctctcccat
     6841 gatccgcttt cttttgggaa ttattactat gatcggcgct ctggtttttc tgggatgtcc
     6901 attgcgcatg gtcatccgta tggccgccgg agatctgaat gcctatatgg gactgattgg
     6961 ttttgtgctg ggtgtaggca ccggctgtat ctttttgaaa aagggcttca gtctgggacg
     7021 atcctttgat acgaaaaaag aaaacggcta cataattcct gccattttcc tgagtcttct
     7081 ggttttaagc atcgctactc ctctttttgc agcaagtaca gaaggtcccg gaagccttca
     7141 tgctcccatc ctcgtatccc tggcagccgg tctgatcttc ggcgccgctg ctcagaaagc
     7201 aagagcctgc ttcgcaggcg gtatccgcga tgtgatcctg atgaaaaact ttgatctttt
     7261 aagtgtaatt gccggacttt ttggcgtaat gctggtttac aacctggtat ccggcaattt
     7321 taaaccggga tttgagggcc agcctattgc ccatgcagaa actctctgga atatccttgg
     7381 cacctatacc gtaggctttg cagccgtatt agccggaggc tgtcctctgc gccagctgat
     7441 tctggcaggc caggggtctt cggatgccgc tgttaatgtg cttggcatgc tggttggtgc
     7501 agccctgtgc cacaactttg gcctggcttc cagcggaaca ggaaccacgc ctgcgggacg
     7561 gatcatgtgt atcgtatgta ttgcggtgct ctttgc
//
LOCUS       DBMDUH010000129         7554 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_4243_length_7554_cov_8.024822, whole genome shotgun sequence.
ACCESSION   DBMDUH010000129 DBMDUH010000000
VERSION     DBMDUH010000129.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 7554)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7554)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7554
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4243_length_7554_cov_8.024822"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="ACZPCG_10340"
     CDS             complement(<1..923)
                     /locus_tag="ACZPCG_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285490.1"
                     /translation="MKKKSIFGCLAIICLLLVLIPPVLTRAEKHSLITWEKKSKNVVS
                     MYLELQEKEAFEEIKAFSLTLNFDSKEDLSEEPVFKFGSDFKGEKVSVHEERYNEEDQ
                     TMKLYVAGRDTVLEKGEKVLLGTITIKSDENVTISVDENDFQIVDRFHEKGMITEFGD
                     FEPYEMILKKEEETTVPETTVPETTVPETTIPPSYEDSEDEEEVYEYEVQPQKEEAPE
                     PNGQWMQNEKGWWFAPLDGTYPADSWYECSSNGFKAWYYFNAEGYMNSGWFTDRDGNT
                     YFLHDQNDSRAGSMYTGWNWINGKCYYFQDTP"
     gene            complement(935..6442)
                     /locus_tag="ACZPCG_10345"
     CDS             complement(935..6442)
                     /locus_tag="ACZPCG_10345"
                     /inference="COORDINATES: protein motif:HMM:NF024794.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HIR1285491.1"
                     /translation="MKRQLACVLAACIGLQLPLGAFNAYGAQLPESAGPMEALEESKI
                     PGLESDSRDEETQTEETEKETIPTASSSESHRQEDEEDGGTETESVPTASPSEPVKPG
                     KEHRGAVMVEIRTLLPVMSAVDFNVELNEVVSKKARANEDGDIYQDSLTISLMPEEES
                     GEGQQENETFENVPNGMYVLEVTAEGYLPYEQTIEVRDDTSKIMILNDYGDYEYSSSA
                     HPGLIRMGDVDGSGVIDKEDMNALVDAVYEGNLTGMDEEGMDLNRDGEIDLVDLQYFA
                     SLYKGKEGSDNKYPVSTIERSITVQEGDVTLASPSEATKSNATKVEGTVQDLFNNDTE
                     SSITLEHADSEIDEPISEAYPVELELDLSRQPVIGGIVIQSPPDSEGAISTGEVEAEL
                     TDGEKIAVTIDRYARTKKRRSFRSGATAMIDADGAILIDLKNQKPVKKIRIKVTGTVS
                     EGNLAEISQVEFLGDMKDRIPPPAQNIPQNVSAQIGSKIFTLTWDKEVNITGYEVSIT
                     NNGKTEIIETTVNTLTVDEFNGKELLNYETFLVKVRSVNGSWKSSYSAQIAVTPEPDG
                     PPPAPENISIEGGYKSLHISWKKMKDTKSYSLYYRKAGTEDEFTMLGELTGTSTTISG
                     LEDKTEYEIYMIGHNPLGDSPQSVIYSGSTITVEAARMPAYRLINTEPKEGQALTEHI
                     KEVSIVTGSVTSVPGDVADGDPYSLWELDDWDSGAYYSYRGPIVTLDQPYIMDTIAFT
                     AGPNQKNDFNAVRVRAWNGEENEPQIINGRLTVREGLDKNGKKYYEFLADKPFEADKI
                     QLNTAVPSSTRYISIAEIRFYEYDELLNEVLSLYTDDLHMTLREDVTQEQITELRIRA
                     DEVDPVSGEMTPKRDLILKELDNAEKILDHNESYKVIHIDTGVSKKYDSHITFSAGLN
                     GWQPLGIAALAGEKVVVYVGAPGRRTGDNTNLDLYATQYHSEASHLQKKVTSLKVGFN
                     EITVPAVSSLGVEKGGALYIEYTGNNPNEIYAVRVIGGSQYPVLDVTRAETAEERKEL
                     TDAYVAEMAEYVQKIEEMHNENSDSEDSHSAISGLDYDERNCILGATDIVLDQMMFSI
                     PIKQVYKSIAGNGESQDEAAEKLYQSLMAMDEMIHLFYQHKGLNAAPVTGGKTYEKDK
                     LPTTRLNIRYQRMFAGAFMYAGGLHIGIEWDSCALLGGSEPIQSDNGKYVSGSYFGWG
                     IAHEIGHIINEGAYAVAEVTNNYYSILAQAKDTNDSVRFKYEDAYRKVTSGTKGGSSD
                     QLGMYWQLHLAYDDGYNFKTYEDYGEQRKNLIFARIDSYARDISRAPAPDGVKLTLDG
                     ADKDNKLMRLACAAAEKNVLEFFTRWGMIPDAVTRKYAEQFDAEERTIYYINDEARAY
                     RAEGGSSIAESVEVTATAHQDETDPGRVTLTMEAHGKDGAAMSGTLFGYEITRIQRRY
                     GKEERQVVGFTQEDTFTDVISGINNRVVGYEVRGIDWCMMPTKAYVLADEILVSHDGS
                     MDKADWSITVNTWSKTDEEVNGDVNSEENQFNQSCSGTVSSAKTMIDNDLDTVYEGTV
                     KAEERTEDAQAVISLGRIEAIAGVKYTYKGTGEPIRAYSISISEDGTDWKEIKKGTFR
                     LENGVAAVHFDKENDGRYYIYDAAYVKITALGSDRFSASEIDILSPIGDSVQLDQFGI
                     LTEDAVFEHSGSDNGSEEGTAAYSGEKRTGSNATRIPKGSIVFTGRYKGNPAYNMVIL
                     YDEKGNVVGGKDKDGDTAADQLILAPDPKDGQLGEVSEGSWIYYIEPKDQNDMVERPE
                     KVRAELYRVQNGETNEGDRLVSDTPFMAVPAVPDPIPTIKLENSQTPNNGE"
     gene            complement(6610..>7554)
                     /locus_tag="ACZPCG_10350"
     CDS             complement(6610..>7554)
                     /locus_tag="ACZPCG_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HIR1285492.1"
                     /translation="FDPKGKSDREIMAFCQSFMTELYRHIGKDTDVPAGDIGVGGREV
                     GYMFGQYKRLTGLYEGVLTGKGLTFGGSLARTQATGYGLVYMLDEMLKHNGKEIAGKT
                     VIVSGSGNVAIYAVEKAQQFGAKVVAMSDSNGYVYDPEGIKLDVVKEIKEVRRGRIKE
                     YAEAVSSAVYTEGKGIWTVPCDIALPCATQNELNLEDAKALLANGCFAVAEGANMPST
                     REATDLFVEKKILFMPGKAANAGGVATSGLEQSQNSLRLSWTFEEVDERLHKIMADIF
                     AKAADAAERYGVPGNYVAGANIAGFEKVVEAMLGQGIV"
BASE COUNT         1695 a         2069 c         1506 g         2284 t
ORIGIN      
        1 ggagtatcct ggaagtaata gcactttcca ttgatccagt tccagcccgt gtacatagaa
       61 ccggctctgc tgtcgttctg gtcatggaga aaataagtat ttccgtcacg gtctgtaaac
      121 cagccgctgt tcatgtagcc ctcggcatta aaataatacc atgccttaaa accatttgag
      181 gagcattcat accagctgtc tgcaggatat gtgccgtcta aaggagcgaa ccaccagccc
      241 ttttcgttct gcatccactg accgttcggc tcaggagcct cctccttttg aggctggact
      301 tcatactcat aaacctcttc ctcatcctcg ctgtcctcat aagatggagg aatcgtagtt
      361 tccggaacag ttgtttccgg aacggtagtt tctggaacgg ttgtttcttc ctctttttta
      421 aggatcattt catatggctc aaaatctcca aactctgtga tcatcccctt ttcatggaac
      481 cggtctacaa tctggaaatc attctcatct acggaaatcg tcacattttc atccgatttt
      541 atggtaatgg ttccaagaag gaccttttct cccttttcaa ggacggtatc ccttccggct
      601 acatacagct tcatggtttg atcttcctca ttgtaccgtt cttcatgaac cgataccttc
      661 tcccccttaa aatcagatcc gaatttaaaa acaggctcct ctgacagatc ttccttactg
      721 tcaaaattca gtgtcaggct gaaggcctta atttcctcga acgcttcctt ttcctgaagc
      781 tccagataca tacttactac atttttgctc ttcttttccc atgtgatcag gctgtgcttt
      841 tccgcccgtg tcagcacggg cgggatcagc acaagaagaa ggcagataat ggccagacag
      901 ccgaaaattg atttcttctt catatttttc tatcttattc tccgttgttt ggtgtttgac
      961 tgttttccag cttgatggtc ggaataggat ccggaactgc cggaaccgcc ataaatggag
     1021 tatcgcttac aaggcggtcg ccttcattgg tttccccatt ctggacacga taaagctctg
     1081 cccgaacctt ttcaggacgt tccaccatgt cattctgatc cttaggctca atatagtaaa
     1141 tccagcttcc ttcagagacc tctcctaact gtccgtcctt tggatcggga gccaggatca
     1201 gctgatctgc agctgtatct ccgtccttgt cctttccgcc gaccacattg cccttttcat
     1261 catagaggat caccatgtta taagcaggat ttcctttata gcgtcctgta aatacgatgg
     1321 aacccttggg gattctggtt gcattgcttc cagtacgttt ttcgccggaa tacgctgctg
     1381 tgccttcttc actgccatta tcgcttccgg aatgctcaaa caccgcatcc tctgtcagga
     1441 ttccaaactg atccagctga acactgtcgc cgatggggct gagaatatcg atctccgaag
     1501 ctgagaagcg gtcagaacca agagcagtga tcttcacata ggctgcatca tagatatagt
     1561 accttccgtc attttccttg tcaaaatgta cggctgcaac gccattttcc agccggaagg
     1621 ttcctttttt aatctccttc cagtctgttc cgtcttcgct gatggaaatg gagtaagccc
     1681 taatcggctc cccggttcct ttatatgtgt atttcacgcc tgcaatggct tctattctgc
     1741 caagggaaat gactgcctga gcgtcctcgg tcctttcctc cgcttttact gttccctcat
     1801 acacagtatc cagatcattg tcaatcatgg tttttgcgct gctgacagtt ccgctgcagg
     1861 actggttaaa ctggttttcc tcgctgttta catccccgtt tacctcttca tccgttttag
     1921 accaggtatt tacggtaatg ctccagtctg ccttatccat gcttccgtca tgggaaacca
     1981 ggatctcatc agccaggaca taagcctttg taggcatcat gcaccagtcg attcctctta
     2041 cctcgtatcc aaccaccctg ttattgattc cggaaatcac atctgtaaag gtatcctcct
     2101 gggtaaagcc cacaacctgg cgctcttcct ttccgtagcg gcgctggatc cgggtaatct
     2161 catagccgaa aagcgttcca ctcatggcag ctccgtcttt tccatgagct tccatggtaa
     2221 gggtcacgcg tcctggatct gtttcatcct gatgcgctgt ggctgttact tctacagatt
     2281 ctgcgatact gcttccgccc tctgcacggt atgccctggc ctcatcattg atatagtaaa
     2341 tcgttctctc ttctgcgtca aactgctctg cgtatttcct tgtaaccgca tcagggatca
     2401 ttccccagcg tgtgaagaat tcaagcacgt tcttttcagc agccgcgcag gccagacgca
     2461 taagcttatt gtccttatcc gctccgtcca gagtcagctt tactccatcc ggtgcaggtg
     2521 cacggcttat atctcttgcg taagaatcaa tgcgggcaaa gattaggttc tttctctgct
     2581 ccccataatc ctcataggtc ttaaagttat atccatcgtc ataagccaga tgaagctgcc
     2641 agtacattcc cagctggtcg ctgcttccgc cctttgttcc tgaagttacc tttctgtaag
     2701 cgtcctcata tttgaaacgc acagaatcgt tggtatcctt tgcctgagcc aggatcgaat
     2761 agtagttatt tgtgacctca gccacagcgt acgcgccttc gttgatgata tggccgattt
     2821 catgggcaat tccccatcca aagtagcttc cgcttacata tttgccgtta tccgactgga
     2881 tcggctctga tccacctaac agcgcgcagg aatcccactc aataccaata tgaagaccgc
     2941 ctgcatacat aaatgcgcct gcaaacattc tctgataacg gatatttaaa cgtgttgttg
     3001 gaagcttgtc cttctcatag gtttttcctc cggtaaccgg agccgcattt aatcccttat
     3061 gctgatagaa cagatgaatc atctcatcca tagccataag agactgatac agcttctctg
     3121 ctgcttcatc ctgagattca ccatttcccg caatggactt atatacctgc ttaatgggaa
     3181 tcgaaaacat catctgatcc aatacaatat ccgttgcacc taaaatacag tttctttcgt
     3241 catagtccag accggaaatg gcgctgtgag agtcctcact gtcagagttc tcattatgca
     3301 tctcctctat cttctggacg tattccgcca tttctgccac ataggcgtct gtcagttctt
     3361 tgcgttcctc tgcagtctct gcccgtgtca catccagaac cggatactga gaaccgccga
     3421 ttacgcggac tgcataaatc tcattggggt tatttccggt atactctata tacagagctc
     3481 cgcctttttc cacacccaga cttgaaacgg caggaactgt aatttcatta aagcctacct
     3541 tcagggatgt tacttttttc tgtaaatggc tggcttctga atggtactga gttgcataaa
     3601 gatccagatt tgtattgtct ccagtacgtc ttccgggagc tcctacatag actacaacct
     3661 tctctccggc cagagctgct attcccagag gctgccatcc attgagaccg gcgctgaagg
     3721 taatatggct gtcatatttc ttggataccc cagtatctat atgaatcacc ttataagact
     3781 cattatgatc caggatcttc tccgcattgt ccagctcctt caggatcaga tctctcttgg
     3841 gagtcatttc tccgcttaca ggatccacct cgtcagccct gatcctcagc tcagtgatct
     3901 gttcctgtgt aacatcctcg cgaagggtca tatgcagatc gtccgtatac agggacagaa
     3961 cctcatttaa aagctcatca tactcataaa aacggatttc tgcaatactg atatagcggg
     4021 tagagctggg tacagccgta ttaagctgaa tcttatctgc ctcaaatggc ttatctgcca
     4081 gaaattcata atatttcttt ccgtttttat ccagaccctc ccggacagtg agacgcccgt
     4141 ttatgatctg aggttcattt tcttctccgt tccaggcacg gacacggact gcattaaaat
     4201 catttttctg attcgggcct gcagtaaatg caatagtatc cataatatag ggctgatcca
     4261 gcgtcacaat agggcctctg taactatagt aagcgccgga atcccagtca tccagctccc
     4321 ataaagaata gggatcaccg tctgccacat cccccggaac acttgttaca gaaccggtga
     4381 caatggatac ctctttaata tgctctgtta atgcctgtcc ttccttcggc tctgtgttga
     4441 tcagacggta agcgggcatt cttgccgctt ccactgtaat cgtacttcca gagtatatca
     4501 cagactgagg actgtctccc aaaggattat gaccgatcat atagatctca tactccgtct
     4561 tatcttccag accgctgatg gttgtgcttg tgcctgtaag ctcaccaagc attgtaaact
     4621 cgtcttctgt acccgccttg cggtaataca ggctgtagct ctttgtatcc ttcatctttt
     4681 tccatgagat atgaaggctc ttatagcctc cctcaatgga aatattttcc ggtgctggcg
     4741 gcggaccgtc cggttccggt gttacggcaa tctgagcgct gtaactgctc ttccagcttc
     4801 cgtttacaga acgaaccttt accagaaacg tctcataatt caacagttct tttccattaa
     4861 attcatcaac tgtcagcgta tttaccgttg tttcgataat ttctgttttt ccattatttg
     4921 taatggaaac ctcatagcct gtaatattta cctctttatc ccatgtaagg gtaaagatct
     4981 tgcttccgat ctgtgcgctt acattctgag ggatattctg ggctggcggc gggatgcggt
     5041 ccttcatatc gcctaaaaat tccacctggc tgatttctgc cagatttcct tccgatacgg
     5101 ttcccgttac cttaatacgg atctttttca caggcttctg attcttcaga tcaatcagga
     5161 tcgcaccgtc tgcatcgatc atggcagtcg caccgcttcg gaaggaacgg cgctttttag
     5221 ttctcgcata cctgtcaatg gttactgcga tcttctcgcc gtcagtcagc tctgcttcta
     5281 cctcaccagt gctgatcgcg ccctcggaat caggcggaga ctgaattaca attcctccga
     5341 tcaccggctg tctggaaaga tccagctcca gttcaaccgg ataagcctct gaaattggtt
     5401 catctatctc gctgtccgcg tgctccagtg ttatggagct ttctgtatca ttattaaaca
     5461 gatcctgtac cgttccctct acctttgtgg cattgctttt tgtcgcttca cttggagatg
     5521 caagggttac atcgccttcc tgaaccgtga tcgaacgttc aatcgtagaa accggatatt
     5581 tattatcgct gccttccttt cctttataaa ggctggcaaa atactgcaga tccaccaggt
     5641 cgatttctcc gtcacggttt aaatccatgc cttcctcatc catacctgtg agattgccct
     5701 cataaacagc atctaccaga gcattcatat cctctttatc aatcacgccg cttccgtcca
     5761 catcacccat acggatcagg cccggatgag cggaggaaga atattcataa tcaccgtaat
     5821 catttaaaat catgatcttg gaggtatcat ccctgacctc tatggtctgt tcatatggaa
     5881 gatatccttc ggctgttacc tcaagcacat acattccatt tggaacattc tcaaaggtct
     5941 cattttcctg ctgtccctct cccgattcct cttcaggcat caggctgatg gtaagagaat
     6001 cctggtaaat atccccgtct tcgttcgctc ttgccttttt ggatacgact tcatttaatt
     6061 caacattaaa atccacagca gacattacag gcagcagggt ccgtatttcc accataacag
     6121 cgcctctgtg ctcttttccc ggctttaccg gctcactggg acttgcagtg ggaactgatt
     6181 ctgtctctgt tcctccatcc tcttcatctt cctgccgatg gctttcagat gaacttgcag
     6241 tgggaatcgt ttctttctct gtttcctcag tctgcgtttc ctcgtccctg ctgtctgatt
     6301 ccagaccggg gattttgctt tcctccaggg cttccattgg ccctgctgat tccggcagct
     6361 gcgctccata ggcgttaaag gcgccaaggg gaagctgcag gccaatacag gcagccagca
     6421 cacatgccag ttgtcgtttc atttctgctt ctcctcttct tttttgtttc gtttttgtaa
     6481 ccaaatgcac cttagtatat caggaaatgt ttgtcatttc aattgttttt ttacttttat
     6541 ttccatataa aaactcccgc gcatataccg tttccgttta tgcgcgggag ttcatttttt
     6601 cattctgttt tatacaattc cctgacccag catagcctca acaaccttct caaagcctgc
     6661 aatattggcg cctgccacat agtttccggg aacgccgtag cgctctgccg catccgcagc
     6721 ctttgcaaaa atatcggcca tgatcttatg aagtctctca tctacttcct caaaggtcca
     6781 ggacaatctt aaagagttct ggctctgctc cagaccggag gttgcaacgc cgcctgcatt
     6841 tgctgcctta ccgggcataa agagaatctt cttctcaaca aagagatcag ttgcttctct
     6901 ggtggaaggc atatttgcac cctctgccac tgcaaagcag ccatttgcca gaagagcttt
     6961 tgcatcctcc agattcagct cgttctgcgt tgcacatgga agagcaatat cgcagggaac
     7021 tgtccagatg ccttttcctt ctgtatatac agcagaagaa actgcctctg catactcctt
     7081 aatgcggcct ctgcgcactt ccttgatctc ctttacaaca tccagcttaa tgccttccgg
     7141 atcgtataca tagccgttgg aatcgctcat ggcaactacc tttgcgccaa actgctgtgc
     7201 cttctcaact gcatagatgg caacatttcc ggaaccagat acgattaccg tctttcctgc
     7261 aatctccttg ccgttgtgct ttaacatctc atccagcatg tatacaaggc catagccggt
     7321 cgcctgggtt cttgccagag aaccgccgaa ggtcagtccc tttccggtga gaacgccctc
     7381 atataagccg gtcagtctct tgtactggcc gaacatatat ccgacctctc ttccgcctac
     7441 gccgatatcg cctgcgggaa cgtccgtatc cttgccgata tgacggtaca gttctgtcat
     7501 aaagctctgg cagaaggcca tgatctcacg gtcggatttg ccctttggat caaa
//
LOCUS       DBMDUH010000130         7458 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_4303_length_7458_cov_6.890852, whole genome shotgun sequence.
ACCESSION   DBMDUH010000130 DBMDUH010000000
VERSION     DBMDUH010000130.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 7458)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7458)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7458
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4303_length_7458_cov_6.890852"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="ACZPCG_10355"
     CDS             <1..816
                     /locus_tag="ACZPCG_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004082434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1285493.1"
                     /translation="EYIQIEAFNQDTLRTLNLYDNNNGYNNAAAILADTNYFPGIDIV
                     KFGENISIIQKRATFENRSILEVYEKSIDVFRDYYQYEVIQGADREKVEKIPEAAFRE
                     AIANALIHRAWDIASQIRVLMFDDRIEVISPGGLPSGITEEEYLSGKLSVLRNRNLAN
                     VFYRLGFVEIFGTGITRIKHLYEEGLIKPDFEVSENTIKIVLPVFEKNLNLTENEQTV
                     YKFLSKTMLKPISEIAPYVPFGRSKTTQLLKGMEQKGIVVVEGKGRGTKYILK"
     gene            complement(966..1910)
                     /locus_tag="ACZPCG_10360"
     CDS             complement(966..1910)
                     /locus_tag="ACZPCG_10360"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HIR1285494.1"
                     /translation="MGMKCIGVDVGGTSVKMGIFETTGELLDKWEVKTRKEENGKYIL
                     PDVAASIREKVSAMGLDLKTDIAGAGLGVPGPVMPDGSVEVCVNLGWKNVNPQKELSA
                     LLDGIPVKSGNDANVAALGEIWQGGGKGYDNLVMITLGTGVGGGVILDQKIVAGRHGL
                     AGEIGHIHVRDEEKEYCNCGGQGCLEQICSATGIAREARRKMAASETASVLRQYGDNV
                     SAKNVLDAAKEGDELALEVMAVVSHYLGLALSQAALIADPDIFVIGGGVSKAGQFLID
                     MVQKDYDHFMTISKNKARLGLATLGNDAGIYGAARLIL"
     gene            complement(1947..3236)
                     /locus_tag="ACZPCG_10365"
     CDS             complement(1947..3236)
                     /locus_tag="ACZPCG_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5711 family protein"
                     /protein_id="HIR1285495.1"
                     /translation="MGSNRSMSRENKKKQLRSQMVSSSVSSPEDEDSEELIKKAHQKS
                     VKRRVRIGAVIGVLILAAVGGFIYYDRYMSFADYELVWETDFNQGEPGEAAAGEGNFS
                     GFADFADGVIKYTKDGASYLDSRGKAVWILSYEMKHPMITVNGDFAAIGDRQGNSIYI
                     CDKNGIQGQATTSLPVLELSVSAKGVTAAVEEDSKASYIYLYKKDGNPLDIYVKSLLS
                     GDGYPVDVSLSPGGTQWITSFMYLEDGMIKNKVVFYNFGLGKNDPKRVVGVFMPQDLS
                     DAMAGRVRFMDDSHAVIFTDKGLQFFSTRIETSPESTAQILLDENIRSISYTDQYAAV
                     VTDNSEGSEPYRLHVYRADGSQVFETAFSFQYSGFDIDEDLVLLYNDNSCLVYNMAGT
                     EKFKGSFDFPVSKVSAGNMPGTLLVMGPQKMQEIRLR"
     gene            complement(3306..4445)
                     /locus_tag="ACZPCG_10370"
     CDS             complement(3306..4445)
                     /locus_tag="ACZPCG_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR1285496.1"
                     /translation="MGELYELFPKLPKNIRQIGERDQVLKLYVEDYVNTYLKQLRPAK
                     GADLRVGLLLGNREIHEDVPYVFVDGALEMETVAKDGERAVFTEDAWQKAYQEVENRF
                     PKRTVQGWFLCGNPGCSLNPLNYWQEHSHYFTGKNQLMYLNSGLEGEEAVYITSSDGF
                     YKLRGYSIYYERNQMMQDYMISRKDISRVETGVNDRAIQDFRQKMEERRGEAIRRQST
                     VGTLSAVCTALAVTVLAGGVAMFNNYKKMHEMESVIASVLPEGRIKEAKAGVWPIEEK
                     FPKVTVGEKPSFVTPETMREAAETETAVISSEAPENVPEETALMETEEADAPAYKVYT
                     VGEGETLYGICFKVYQNVNKLDEICRINELRDQNSIYAGQKLLMP"
     gene            complement(4622..5131)
                     /gene="yyaC"
                     /locus_tag="ACZPCG_10375"
     CDS             complement(4622..5131)
                     /gene="yyaC"
                     /locus_tag="ACZPCG_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HIR1285497.1"
                     /translation="MNLNRMIMDEMEEKGKEGVMFLCIGTDRSTGDSLGPLVGHKLRR
                     QRLKGAAVIGTLDKPVHAMNLELYIRYIQAHYSRYVVVAIDASVGSLDHVGYATLGRG
                     ALQPGLGVSKELEAVGDISITGIVGGAGSRDPVMLQSVRLSMVMKMADCICESISLVE
                     RFWENAAII"
     gene            complement(5312..6232)
                     /locus_tag="ACZPCG_10380"
     CDS             complement(5312..6232)
                     /locus_tag="ACZPCG_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HIR1285498.1"
                     /translation="MKETALVIMAAGIGSRFGGGIKQLAPVGPNGEIIMDYSIHDAIE
                     AGFNKVVFIIRKDLEKDFKEIIGDRIAKQIPVIYAYQELDALPEGYFVPEGRKKPWGT
                     GQAVLMIKGLIDCPFLVINADDYYGKEGFKRIHDYMVNEMKDDADVYDICMGGFVLEN
                     TLSDNGSVTRGVCHVENGFLKNVTETYDIRRSGDGLAAADEAGNPVSVQADQPVSMNM
                     WGLPVRFLEELEHGFPKFLDGLKPEDIKAEYLLPKIIDDLVQEGKAKVRVLDTPDKWF
                     GVTYQEDKQAVVDAIRGLIRDGVYKEKLFG"
     gene            complement(6313..>7458)
                     /locus_tag="ACZPCG_10385"
     CDS             complement(6313..>7458)
                     /locus_tag="ACZPCG_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HIR1285499.1"
                     /translation="GERLYEYLSRCCERCGGSLADQIQIAREEVLPGRFNVMARIPGK
                     TNGPGLIYICHMDTVMLGDGWDETTPPLGAVVRETEEQGKTVVRLYGRGACDMKSGLA
                     CALAAFEAQVRETGKRGELPNRSFSIICTVDEEDFMRGVEHAIRSGWITKEDWVLDTE
                     PTDGQIQVAHKGRTWFELTVTGITAHASNPWKGADAIAAMAEVISSIRKEIAACPPHH
                     DLGISTVTFGQIQGGYRPYVVPDSCKVWIDMRLAPPVDTAAACAMVEGAIKKAEKEIA
                     GVKGSYVLTGDRPYVEKDPDSPLLSRLKEAVKAVVGEEAAVGTFNGYTDTAVAAGMLG
                     NHNCMSYGPGSLELAHKPNESVPYEDVKRVQGVLTELAGNILWRKPM"
BASE COUNT         1874 a         2111 c         1445 g         2028 t
ORIGIN      
        1 gagtatatcc aaatcgaagc attcaatcag gatacattaa gaacattaaa cttatatgac
       61 aataacaatg gatataataa tgcagcagca attctagccg ataccaatta ttttccggga
      121 attgatattg taaaattcgg tgaaaatatc agcatcattc aaaaaagggc aacctttgaa
      181 aacagatcga tattggaagt ttatgaaaag tcgatcgatg tattcagaga ttattatcaa
      241 tatgaagtaa tacagggtgc tgaccgggaa aaggtagaaa aaataccaga agcggctttt
      301 cgggaagcga ttgcaaatgc tttgattcac agagcctggg atatagcatc gcaaatccgg
      361 gttttaatgt ttgatgaccg gattgaggtt atctctccgg gagggctacc atccggaatc
      421 acagaggaag aatatttatc aggaaaactt tctgtattaa gaaacaggaa cctcgcgaat
      481 gtattttaca gattaggctt tgtcgaaata ttcggaacag gaattacaag aataaaacat
      541 ctttatgaag aaggacttat caaaccggat tttgaagtat ccgaaaatac aattaaaatt
      601 gtgcttcctg tttttgagaa aaatctgaat ttaacggaaa acgaacaaac ggtatataaa
      661 tttttaagta aaacaatgct gaaaccaatc agtgaaattg ccccctatgt cccctttgga
      721 agatcaaaga caacgcagct tctgaaaggt atggagcaaa aaggaattgt ggtcgttgaa
      781 gggaagggac gggggacaaa gtatatactg aaatagatca tagtttaaaa acgaatgtcc
      841 agcctgccaa aaaaagcggg aagattacca gagaaaatgt gatgtctctc tccggtatct
      901 tcccgccttt tattatgacc tcgaccgtac ctgtttccaa tctttccctc ttaaatttcc
      961 cctcactaca aaatcagtct tgctgcccca tagattcctg cgtcatttcc cagagttgcc
     1021 agacccagcc tggctttatt tttggaaatc gtcataaaat gatcatagtc cttctgaacc
     1081 atatcaataa ggaactggcc tgcttttgat acgccgccgc cgatcacgaa aatatcagga
     1141 tctgcgatca gagcagcctg ggacagcgcc agacccagat aatggcttac cacagccata
     1201 acctccaatg ccagttcgtc tccctccttg gccgcatcca gcacgttttt cgcacttaca
     1261 ttgtcaccgt actggcggag aacggaagcc gtctcgctgg cagccatctt tcttctggct
     1321 tctcttgcaa tgcccgtagc ggagcagatc tgctccagac atccctgacc gccgcagttg
     1381 cagtattcct tttcctcgtc ccttacatgg atatgtccga tctctccggc cagaccgtga
     1441 cgtccggcca cgatcttctg atccaggatc actcctccgc ctactccggt accaagagta
     1501 atcatcacaa ggttatcata gccttttccg cctccctgcc atatctcgcc aagggcagct
     1561 acattggcat catttccgct ctttacagga atgccatcca gcagggcact gagctccttc
     1621 tggggattta catttttcca tcccagattt acgcagacct ctacgcttcc gtccggcatc
     1681 accggccccg gcactcccag tccggctcct gcaatatccg ttttcagatc cagccccatg
     1741 gctgatacct tttcacggat ggaagcagcg acatccggaa gaatatattt tccattttct
     1801 tcctttcttg tttttacctc ccacttatcc agcaattctc cggttgtctc aaagattccc
     1861 atctttacgc tggttcctcc tacgtctaca ccgatgcatt tcatccccat agtatcttct
     1921 tcctttctgt ctgatttttg cagctgttac cgcagcctga tctcctgcat cttctgaggc
     1981 cccatgacta aaagagtacc cggcatattt ccggccgaaa ccttggatac aggaaaatca
     2041 aaacttcctt taaatttttc tgttcccgcc atattataaa ccaggcagga gttgtcatta
     2101 taaagcagaa ccagatcctc atctatatca aaacccgaat actggaacga gaacgcagtc
     2161 tcaaacacct gactgccgtc tgcccggtac acatgaagcc gataaggctc tgaaccctcg
     2221 gaattatctg tgaccacagc cgcatactga tcggtataac tgatactgcg gatattttcg
     2281 tctaacagga tctgagccgt ggattccgga gacgtttcaa tacgggtcga aaaaaactgc
     2341 agtcccttgt ccgtaaaaat caccgcatgg ctgtcatcca taaaccgcac ccggcctgcc
     2401 atggcgtctg aaagatcctg gggcataaat acgccgacta cacgcttggg atcatttttt
     2461 cccagaccaa agttgtaaaa tacaacctta tttttaatca tgccatcttc taaatacata
     2521 aaagaggtga tccactgggt tcctccggga gacaggctta catcaactgg ataaccgtcg
     2581 ccggacagaa gcgatttcac atagatatca agaggatttc cgtccttttt ataaaggtaa
     2641 atgtagctgg ctttagaatc ctcctccaca gccgcagtaa cgccctttgc tgaaacagaa
     2701 agctctaaaa ccggcagaga agttgtcgcc tgaccctgaa ttccattttt atcacagata
     2761 taaatggaat ttccctgacg gtctccaatg gcggcaaaat caccgttgac cgtaatcatg
     2821 ggatgtttca tctcatagct taaaatccat acggcctttc ccctggaatc cagataggag
     2881 gctccatcct tggtatactt gatcacaccg tcggcaaaat ctgcaaatcc ggaaaaattc
     2941 ccttccccgg cagcagcttc gcccggctct ccctgattaa aatccgtctc ccagacaagc
     3001 tcatagtctg caaatgacat atatcggtca taatatataa atccgcccac agcggccaaa
     3061 atcagaaccc cgatcactgc tccaatgcgg acccggcgtt ttacgctttt ctgatgggct
     3121 ttctttatca gctcttcgct gtcctcatcc tccggcgaag aaacggagga agaaaccatc
     3181 tggcttctca gctgcttttt tttattttca cggctcattg aacggttgct gcccattttg
     3241 tactcctgaa tgtttgatat acctgttaag tatacaacat tttcggaggt tgcgcactaa
     3301 tattttcacg gcataagaag cttctggcct gcatagatgc tgttctgatc cctcaattcg
     3361 ttgatccggc agatctcgtc cagcttattt acattttggt aaaccttaaa acagatgcca
     3421 taaagtgttt cgccctcccc aacggtataa accttatagg cgggagcatc agcttcctcc
     3481 gtttccatga gtgctgtttc ttccgggaca ttctccggag cttcactgga aatgactgca
     3541 gtttcagtct ccgcagcttc tctcatcgtt tcaggagtca caaaagaagg cttttccccc
     3601 accgtaacct tagggaattt ttcctctata ggccagaccc ctgcttttgc ttccttgatc
     3661 cttccctccg ggagcaccga tgcaatcaca ctttccattt catgcatctt tttatagtta
     3721 ttaaacatcg ctacaccgcc tgccaaaaca gttacagcca aagctgtgca cacagccgaa
     3781 agcgtaccca ccgtactctg gcggcggatt gcttctcctc tgcgctcttc cattttttgg
     3841 cgaaaatcct ggatggcgcg gtcatttacc cctgtttcca ctctggaaat atcctttcgg
     3901 gaaatcatat aatcctgcat catctgattg cgctcatagt aaatactgta acccctgagt
     3961 ttataaaatc cgtctgagga cgtaatatag accgcttcct caccctccag gccgctgttt
     4021 aaatacatca gctgattttt tcctgtaaaa taatggctgt gttcctgcca gtagtttaat
     4081 ggatttaaac tgcagcccgg attcccgcat aaaaaccacc cctgtaccgt tcgtttggga
     4141 aaccggtttt ccacttcctg ataggccttt tgccatgcat cctctgtaaa gactgccctc
     4201 tccccatcct ttgcaaccgt ttccatttcc aaagccccgt ctacaaatac atacggcaca
     4261 tcctcatgga tctccctgtt tcccagaaga agccccaccc gcagatccgc cccttttgcg
     4321 ggccttaact gctttaaata tgtattgaca taatcctcta catacagctt taaaacctgg
     4381 tcacgttctc cgatctgccg tatatttttc ggcagcttcg ggaacagttc atataattct
     4441 cccatctgct caccccttta taccgatgcg aaacctataa agcgcgtttc gcctctgatc
     4501 agcggccgtc aaatgtctgc acacgcaggc gtgacagcca ttttcctcgt cgccacaaca
     4561 aaaaacatac ctgaaaaatc caggtatgtc tcagtataac aggtattttg cgcaaagttt
     4621 gtcatattat ggctgcattt tcccaaaatc gttcgacaag ggaaatactt tcgcagatgc
     4681 agtccgccat tttcatcacc atggacagcc ggacactttg aagcatcaca ggatcacggc
     4741 tgcctgcgcc ccctacgatc ccggtgatcg agatatctcc tactgcctct aactcctttg
     4801 ataccccaag ccccggctgc agcgctcccc gccccaaagt agcatagccc acatgatcca
     4861 ggctgcctac cgaagcgtca atcgctacca ccacataccg ggaataatgg gcctggatat
     4921 atcggatata aagctccaga ttcatggcat gtaccggctt atccagagtt ccgatcacag
     4981 cggctccctt taaacgctgc cttctcagct tgtgtccaac caaaggcccc aggctgtccc
     5041 ctgtagagcg gtctgtgccg atacagagga acatgacgcc ttcttttcct ttttcttcca
     5101 tttcgtccat gatcatacgg ttcaggttca cggcaaaacg ttctgcctca aactctcccg
     5161 ctgtatcata gtatgatata tcttcccggc tggaaatacc gaatgttttc cacaatttca
     5221 tgtctgtact gtctccttat ccacggaggc tgaactattt tcaccgtttt ttatagtata
     5281 gaccctgaaa atgggaaata tgcatggctt tttaaccgaa cagcttttct ttgtatacgc
     5341 cgtcccggat cagtccccgg atcgcgtcta ccaccgcctg cttatcctcc tgataggtca
     5401 caccgaacca tttatccggt gtatccaata cgcggacttt cgccttcccc tcctgtacca
     5461 gatcgtcaat aatcttcggg agcaggtatt ctgccttaat atcctcaggc tttaagccgt
     5521 ctaaaaactt cgggaaccca tgttccagct cctctaagaa acgcaccgga agaccccaca
     5581 tattcatgga aaccggctga tctgcctgta cagatacagg atttcccgcc tcatctgctg
     5641 ctgccagtcc gtccccgctt ctgcggatat cataagtttc cgttacgttt ttaaggaatc
     5701 cgttttccac atgacacacg cctctggtaa cgcttccgtt gtcgcttaag gtattttcaa
     5761 gcacaaatcc gcccatgcag atatcataaa catctgcatc atccttcatc tcatttacca
     5821 tgtaatcgtg gatccgctta aagccctctt ttccatagta atcgtctgca ttgatcacca
     5881 ggaacggaca gtcaatcagc ccctttatca tcagaacagc ctgaccggtt ccccagggtt
     5941 ttttgcggcc ctcaggcaca aaatagccct ctggaagagc gtccagctcc tgatatgcat
     6001 aaataaccgg gatctgcttg gcaatacggt ctccgataat ctccttaaaa tccttttcca
     6061 gatctttacg gataataaat acgactttgt taaatccggc ctcaatggcg tcatggatag
     6121 aataatccat aataatctct ccatttggcc caacaggagc cagctgcttg attccgcccc
     6181 caaaccggct gccgattccg gccgccataa ttactaatgc tgtttccttc ataatcttgt
     6241 ttcctccttt taaggtgcca ctattatact acaaaatcct tattgatgga aatggtattt
     6301 tccctgatac ttctacatgg gtttcctcca caaaatattc ccggccagct ctgtaagcac
     6361 cccctggacc cgtttcacat cctcataagg tacggattca ttaggcttat gagccagctc
     6421 caggctgccg gggccatatg acatacagtt atgatttccc agcatccctg ctgctaccgc
     6481 cgtatctgtg tagccgttaa aggttcctac cgccgcttcc tctcccacaa cggcctttac
     6541 agcctctttt aaacgggaca acagcggtga atccgggtcc ttttctacat agggtctgtc
     6601 tcctgtaagc acatagcttc ccttaactcc tgcgatttcc ttctctgcct tttttatcgc
     6661 cccttcaacc atcgcacagg cagccgccgt atccactggg ggcgccagac gcatatcaat
     6721 ccatacttta cagctgtccg gcactacata aggcctgtag cctccctgga tctggccaaa
     6781 agtaacggta gaaatcccca gatcatggtg gggcggacag gctgcaatct cctttcgtat
     6841 gctgctgatc acctccgcca tggctgcaat ggcgtctgct cccttccatg gattgctggc
     6901 gtgggcggtg atgcccgtaa cggtcagttc aaaccaggtg cggcccttgt gagccacctg
     6961 aatctgtcca tctgtaggct ctgtatccaa aacccagtcc tccttcgtga tccagccgga
     7021 acggatggca tgctccactc cccgcataaa atcctcttca tccacggtac agattatgga
     7081 aaatgaccgg ttaggaagct ctcccctttt tccggtctcc cgtacctgag cctcaaaggc
     7141 agcaagggca caggctaacc ccgacttcat atcacaggct cctcttccat acaggcgcac
     7201 caccgttttt ccctgctcct ctgtttcccg gaccaccgct cccagaggcg gcgtcgtttc
     7261 atcccagcca tctcccagca taaccgtatc catgtggcag atatagatca gtcccggtcc
     7321 attcgtcttt cccggtatcc gcgccatcac attaaaccgg cccggaagta cctcctcccg
     7381 tgcgatttgg atctggtctg ccagagaacc gccacaccgt tcacagcacc ggcttaaata
     7441 ctcataaagc cgttcccc
//
LOCUS       DBMDUH010000131         7456 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_4304_length_7456_cov_6.553909, whole genome shotgun sequence.
ACCESSION   DBMDUH010000131 DBMDUH010000000
VERSION     DBMDUH010000131.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 7456)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7456)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7456
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4304_length_7456_cov_6.553909"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="ACZPCG_10390"
     CDS             <1..573
                     /locus_tag="ACZPCG_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HIR1285500.1"
                     /translation="LGIFVSAALAPKTSDDQPGLLYEGIDYRAIGEAADRVMLMAYEW
                     GYSQGPPMAVAPIHMVRRVADYALSRIPAEKISLGIPNYGYDWPLPYERGRTRAVTLN
                     TRQAIQLAIDHGSVIYFDELAQSPYFHYWQYGIRHEVWFEDVRSLKAKFELIREKKLT
                     GAGFWQLMYFYRPGWLLLTRMFALAKENFI"
     gene            647..1633
                     /locus_tag="ACZPCG_10395"
     CDS             647..1633
                     /locus_tag="ACZPCG_10395"
                     /EC_number="1.4.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003525383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047850 - diaminopimelate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate dehydrogenase"
                     /protein_id="HIR1285501.1"
                     /translation="MSIRIGILGYGNLGRGVECAVKHNPDMELKAVFTRRDPSSLSLL
                     TEGVKVYSVNDILTMKDEIDVMIICGGSATDLPKQTPEMAKYFNVIDSFDTHAKIPEH
                     FEAVDRSAFESGHVAVISVGWDPGMFSLNRLYADAVLPGGKDYTFWGRGVSQGHSDAI
                     RRIPGVRDARQYTIPVESALEAVRSGSQPELTTRQKHTRECFVVAEEGADQDAIREAI
                     VTMPNYFADYDTTVHFISQEELLRDHAGIPHGGFVIRTGTTGMKDEHSHVIEYSLKLD
                     SNPEFTASVLAAYARAAYRMNKEGAKGCKTVFDIAPAYLSPKDGAYLRKHML"
     gene            1644..3653
                     /locus_tag="ACZPCG_10400"
     CDS             1644..3653
                     /locus_tag="ACZPCG_10400"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-containing oxidoreductase family
                     protein"
                     /protein_id="HIR1285502.1"
                     /translation="MTDIRRTTCPYDCPASCGLLATIENGRVAGVAGDPEHPASKGLI
                     CKKMQQYQLSVNSPDRILFPMRRTGNKGEGKFEPITWEEAVREIAGHFRQIIRENGPL
                     AILPSYYSGTMGVIQRKCGDAFFNRLGARPLILRLCANAKGMGYASVMGQTPCLEPEE
                     LKSSDLILVWSSNVKATRLHVMPVLKEARAAGKKVLLIEACAREMDAYCDETILIRPG
                     TDGALALAMMHVLEEKGLADEVFLRERCEGYEAFLPIVRKSTPKWAEQITGIPEKNIR
                     RLALFFGQAKAPAILLGSGFSRYGNGGMTSRLITVLSAFTGAWGKPGGGICAAEHNGG
                     PFVDAKRVTRPDFRKVSGPGVNINCLGSALTAEGEDAVKAFFVYGGNPVGSVCDQKAI
                     LKGLMREDLFTVVHERFLTDTARYADILLPAVFSVEQTDCFRAYGYRTFSVAKKLVEP
                     QGECKSNWELFGLLAEAMGFEEPYFKRTEEEMLEELLSHPEEGLLELTEKQWKFLREN
                     GTVRQPFGNHGHFKTPSGKLRIVDQEEKEPVPCYKEPYGGAYPLSLISIPDSHTLNSI
                     FLERRDLVQKRGPAALMLHPLDAAERKVREGDMVTAWNDLAEVDFRAVITDRIAKGTA
                     AVSGIYSSSQTGSALLFNALNHERLSDMEATTLNDNRIDVKRRMA"
     gene            3680..4951
                     /locus_tag="ACZPCG_10405"
     CDS             3680..4951
                     /locus_tag="ACZPCG_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003510052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HIR1285503.1"
                     /translation="MKTLTRKGKFLILSLFPLYFIVIGLVVQPFPDILKGLIRIVIEP
                     DLLITDYFAVGGIGAAMINAGIITLISLAMIYFLKMEMSGHTITAVFLMFGFSLFGKN
                     IMNIWAILAGVFLYSRYHKTSFHRYLYIGLYGTSLSPIMTQIMQIGHLPVYARVLISL
                     AVGISIGFVLPPLSTHVHYAHNGYSLYNVGFAAGIIATVVVSLEKSFGIRIESRLIWS
                     KGNQELFAVLLSLFFGTMIAAAVAVRGKTLWESYRRILADPGLAGADFFKAEGGATTV
                     FNMGINGLFATFFVLMVGGDMNGPTICGILTIVGFSSTGKHIRNIAPVMFGVYLASFT
                     KKWNINDPSPILALLLSTTLAPVAGKFGVVAGLIAGYLHSSVALQVGIVYGGVNLYNN
                     GFAGGIVAIFLVPVIQSICDRRARARGGLSL"
     gene            5031..5279
                     /locus_tag="ACZPCG_10410"
     CDS             5031..5279
                     /locus_tag="ACZPCG_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285504.1"
                     /translation="MEDRRYDDTVENPKDRGGFLWGLLGCCIPVVGLVLFLVWRDEKP
                     KTAKAAGIGALVSVILTILIYVGVFVMSIATAALIGGM"
     gene            5293..5688
                     /locus_tag="ACZPCG_10415"
     CDS             5293..5688
                     /locus_tag="ACZPCG_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006778568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HIR1285505.1"
                     /translation="MGRFLRIFFGCHARPDRSFYWNGKPFPICARCTGELAGMIAGIP
                     LILLWGDMKLRLMILFMLPMTVDGFLQLFTSYESKNGRRFATGVLFGLAFDSLLIHFH
                     RACVMAAGWIVKLFIEDSGAVERAMELFL"
     gene            complement(5726..6085)
                     /locus_tag="ACZPCG_10420"
     CDS             complement(5726..6085)
                     /locus_tag="ACZPCG_10420"
                     /inference="COORDINATES: protein motif:HMM:NF018685.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HIR1285506.1"
                     /translation="MARTSAAGRRTCFLIIVLLLLAAVLMGYQYLFPAPKGQTAHIFV
                     DGKEAASFPLNKDLEYRVSGADGGTNLLIIQDGQIWCQEADCPDKNCVRQGKKQLSTD
                     TIICKPHKMAVTITGEE"
     gene            complement(6085..6579)
                     /locus_tag="ACZPCG_10425"
     CDS             complement(6085..6579)
                     /locus_tag="ACZPCG_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285507.1"
                     /translation="MKQNHLMKHNLAGFILMAALSLVFIAASGSIYQLLRGPVTVNQP
                     EFPLKDGQYSYDSEFDSKGFYNHVELTVENGIITACSWDCFGEDGIGKQQLSLDGLYI
                     MTEDGPTWKAQSEALAAYVIEHQTVSQLADENGYAKDAIASVSINVSGFINGINECLK
                     QAAA"
     gene            6668..7174
                     /locus_tag="ACZPCG_10430"
     CDS             6668..7174
                     /locus_tag="ACZPCG_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HIR1285508.1"
                     /translation="MNIRTRRVAVYGLLIALAFVFGYIERILPIYLGAPGVKLGLANL
                     VTILSLYVLGLWPAVWISLARILLSGFAFGGMSSILFSLAGAALSLLVMWICRRFSLF
                     DMVGVSILGGVFHNIGQFLMAAYVVKTFGVFSYLPVLLFAGTAAGAAIGLLGGLVLKR
                     IGNVIKNL"
BASE COUNT         1827 a         1637 c         2062 g         1930 t
ORIGIN      
        1 ttggggattt ttgtcagtgc tgccctggcg ccaaagacct ccgatgacca gccggggctt
       61 ctctatgaag gcattgatta ccgggccatc ggcgaagcgg cagacagggt gatgcttatg
      121 gcctatgagt ggggatattc ccaggggccg cctatggcag tggcgcccat ccatatggtc
      181 cgtcgggtgg cggattatgc attaagcagg atcccggcag aaaagatcag ccttggcatt
      241 ccaaattatg gatatgactg gccccttcct tatgagcggg gacgcacacg ggcagttaca
      301 ttaaataccc gccaggccat acagctggcc attgaccatg gctctgtgat ctattttgat
      361 gagctggcac agtctcccta ttttcattac tggcagtacg gcatccgcca tgaggtatgg
      421 tttgaggatg tgcgaagctt aaaggccaag tttgaactta taagggagaa aaaactcact
      481 ggcgcgggtt tctggcagct gatgtatttt taccgccccg gctggctgct tcttacgcga
      541 atgtttgcgc ttgcaaagga aaatttcata tagtatactt tttacagtgg ccggaacgga
      601 tatctgcatc cggctaaaag accctaagag aaaggaagac aaaattatga gcatcagaat
      661 cggaatcctt ggatacggaa acctgggaag gggagtggag tgtgcagtta agcacaatcc
      721 ggatatggaa ctgaaggcag tgtttacccg gagagaccca tcgtcccttt ctctcctgac
      781 agagggagta aaggtatatt cagttaatga cattctcaca atgaaagatg agattgatgt
      841 aatgatcatc tgcggaggca gcgctacgga tcttccaaag cagacaccgg aaatggcgaa
      901 gtattttaat gtgatcgaca gttttgatac ccacgcaaag attccggagc attttgaggc
      961 agtagaccgg agcgcttttg agagcggcca tgtggcagtg atctctgtgg gctgggatcc
     1021 cggtatgttt tccttaaacc gtctttatgc ggatgcggtc cttcccggag gaaaggacta
     1081 taccttctgg ggcagaggcg taagccaggg acattctgat gccatccgcc ggatccctgg
     1141 tgtaagagat gcgagacagt atacgattcc ggttgagagc gcattggagg cagtgcgcag
     1201 cggaagccag ccggagctta ctacacggca gaagcacacc agagagtgct ttgtagtggc
     1261 agaagaggga gccgatcagg atgcaatccg tgaagccatc gttactatgc ccaattattt
     1321 tgcagattat gacacaacgg tgcattttat cagccaggaa gagcttttaa gagatcatgc
     1381 gggaatccct cacggcggct ttgtgatccg cacaggaact acaggcatga aggatgagca
     1441 ttcccatgtg atcgagtaca gcttaaagct ggattctaat ccggagttta ctgcttccgt
     1501 ccttgccgcc tatgcaagag ctgcctaccg tatgaataag gagggcgcaa agggctgcaa
     1561 gaccgtattt gatattgctc cggcctactt aagcccaaag gacggcgcat atctgaggaa
     1621 gcatatgctg taggaggaat ggaatgactg atatccgcag gactacctgc ccctatgact
     1681 gcccggcctc ctgcggcctt ttagccacca tagagaacgg gcgcgtggca ggggtggccg
     1741 gggatccgga gcatccggcc tcaaaaggat tgatctgtaa aaaaatgcag cagtatcagc
     1801 tgtccgtcaa cagcccggac cggatccttt ttcctatgag acggactggg aataaggggg
     1861 aagggaagtt tgaacctatc acctgggaag aggcggtaag ggagatcgca ggacattttc
     1921 ggcagatcat aagggaaaat ggcccgttgg cgatccttcc gtcctattat tccggcacta
     1981 tgggcgtgat ccagcgaaag tgcggggatg ccttttttaa ccgtctggga gcacgcccac
     2041 ttattttaag actctgcgcc aatgcaaaag gaatgggata tgcctctgtc atggggcaga
     2101 ccccctgcct ggagccggag gaattaaaaa gcagtgatct gatcctggtg tggagcagca
     2161 atgtaaaggc cacacgcctc catgtgatgc ccgttttaaa ggaagcaagg gctgccggga
     2221 aaaaagtgct ccttattgaa gcctgcgcca gagaaatgga cgcctactgt gacgaaacga
     2281 ttctgatccg tcccggtaca gacggagcac tggctcttgc catgatgcat gtgctggagg
     2341 aaaaagggct ggcagatgag gtgtttttaa gagaacggtg tgagggctat gaggcttttt
     2401 tgccgattgt gagaaaaagc actccaaagt gggccgaaca gatcacaggc atacctgaaa
     2461 aaaacatccg caggctggct cttttcttcg gacaggccaa agctccggcg attcttttgg
     2521 gaagcggctt ttccagatat ggaaatggag gaatgacctc ccgcctgatc acggttttat
     2581 ctgcatttac aggcgcctgg ggaaagccgg gcggcggtat ctgcgcagcg gagcataatg
     2641 gcggtccctt cgtggatgcg aaacgtgtta caagaccgga tttcaggaag gtttcagggc
     2701 ctggcgtgaa catcaactgt ctcggttcgg cgcttacggc ggaaggagag gatgcagtga
     2761 aggccttttt cgtctatgga ggcaatcctg tgggctctgt gtgtgatcag aaggcgattc
     2821 ttaaggggct gatgcgggag gatttattta cagtggtaca tgagcggttt ctcaccgata
     2881 cggcccgcta tgcagatatc cttcttccgg cagttttttc cgtagagcag accgactgtt
     2941 tcagggcata tggctatcgg acctttagcg tagcaaaaaa actggttgag cctcagggag
     3001 agtgcaaaag caactgggag ctctttggac ttctggcaga ggccatggga tttgaagagc
     3061 cgtattttaa gcgtaccgag gaagaaatgt tagaggagct tttaagccat cctgaagagg
     3121 ggcttttgga acttacagaa aaacagtgga agtttctgcg ggaaaatggc acagtacgtc
     3181 agccctttgg gaatcatggc cattttaaaa cgccttccgg taagcttcgg attgtcgatc
     3241 aggaggaaaa ggaacctgtg ccctgctata aggaacctta cggcggagcg tatccgttaa
     3301 gcctgatcag catacctgat tcccatacct taaattctat ttttctggag cgcagggatt
     3361 tagtacaaaa gcgggggccg gctgccctga tgctccatcc cctggatgcc gcagaacgaa
     3421 aagtccggga gggagatatg gttactgcat ggaatgatct ggccgaggta gactttcggg
     3481 cggtgatcac agaccggatt gcaaagggaa cggctgctgt ttctggcatt tacagcagtt
     3541 ctcagacagg ctccgccctt ttatttaatg ctctgaacca tgagaggctt tccgatatgg
     3601 aggccacgac actgaatgat aaccggattg atgtaaaaag gaggatggcg tgaaacacca
     3661 cctggaacat tttcatgaaa tgaaaacact gacccggaag ggaaaatttc ttattttatc
     3721 cctgtttcca ctgtatttta ttgtgatcgg ccttgtggtc cagccttttc ctgatatcct
     3781 taaggggctc atccgcattg tcatagaacc cgatcttctg atcacggatt actttgccgt
     3841 aggcggaatc ggagcagcca tgatcaatgc aggaatcatc acgctgatca gtctggcgat
     3901 gatttatttc ctgaaaatgg aaatgagcgg ccatacgatc acggcagtgt ttctcatgtt
     3961 cggcttttcg ctgttcggaa agaatattat gaatatctgg gccattctgg caggtgtatt
     4021 tctgtattcc agatatcata agacatcctt tcaccggtat ctttatatcg gcctttacgg
     4081 aaccagcctt tcgcctatta tgacgcagat catgcagatc ggccatctgc cggtctatgc
     4141 cagagttttg atctctctgg cggtggggat cagcattggt tttgtgctcc cgcctttaag
     4201 tacccatgtc cactatgcgc acaatggata ttccctttat aacgtaggtt ttgcagcagg
     4261 gattatcgct acggtggtcg tatctctgga aaaatccttt gggatccgga ttgaatcaag
     4321 gctgatctgg tccaagggca accaggaact ctttgcagtg cttttgtcct tgtttttcgg
     4381 aactatgatc gcagcagcag ttgctgtgag aggaaagacg ctgtgggaat cctaccgccg
     4441 gatcctggca gatccaggtt tggcgggagc cgactttttt aaggctgagg gcggggcaac
     4501 tacggtcttt aatatgggga tcaatggcct gtttgccacc ttttttgtgc tcatggtagg
     4561 cggagatatg aacgggccta caatctgcgg tatccttacg atcgtgggct tcagctctac
     4621 gggaaaacat atacggaata tagcaccggt tatgtttggc gtatatcttg caagctttac
     4681 caaaaaatgg aatatcaatg atccgtctcc cattctggcc cttcttcttt cgaccacgct
     4741 ggcgccggtt gcgggaaaat tcggagtcgt ggcgggactt attgcagggt atctccattc
     4801 ctctgtggct ttgcaggtgg ggatcgtcta tggaggcgtg aatctttaca ataatgggtt
     4861 tgcaggggga attgtggcaa ttttcctggt gcctgtgatc cagtcgatct gtgaccgcag
     4921 agcaagagca aggggaggac tgtcgctgta ggggagaaag cttaaagcga aggtatcttg
     4981 ccgattaagc aagaaatctg tataatattg agaaaaaggg aggaaatcat atggaagaca
     5041 gaagatacga cgatacggtg gaaaatccaa aagacagagg cggttttttg tggggccttt
     5101 tgggatgctg cattccggtg gtaggcctgg tcttatttct ggtatggaga gatgaaaagc
     5161 ccaaaacggc gaaagcggca ggcatcggag cactggtatc cgtgatcctt accatactca
     5221 tctatgtagg tgtattcgtt atgtccattg ctacggcagc gctgattggc ggaatgtaag
     5281 gagtaaaaga ggatggggcg tttccttcgg atcttttttg gctgtcatgc ccgtccggac
     5341 cgttcctttt actggaacgg aaaaccgttt cctatctgtg cccgatgtac gggggagctg
     5401 gccggcatga tcgccgggat acctctgatc cttctatggg gagatatgaa gctgcggctg
     5461 atgatccttt ttatgcttcc tatgacagta gacggttttt tacagctttt tacatcctat
     5521 gaaagcaaaa atggcaggcg gtttgcaacg ggggttctgt ttggcctggc ctttgacagc
     5581 ctgctcattc atttccacag agcctgtgtg atggcagccg ggtggatcgt aaagctgttt
     5641 atagaggaca gtggggctgt agagagagcc atggaactgt ttttgtgata cggataccag
     5701 agggataaaa aacaggagag gaagtttatt cctctcctgt gatcgttaca gccattttat
     5761 ggggtttaca tatgatggta tcggtggaaa gctgtttttt tccctgccgg acacagtttt
     5821 tatcgggaca gtcggcctcc tgacaccaga tctgtccgtc ttggatgatc aaaagatttg
     5881 ttccgccgtc tgctcccgaa accctgtatt ccagatcctt atttaaagga aaggaagcag
     5941 cctcttttcc gtccacaaaa atatgagccg tctggccttt gggagcaggg aaaagatact
     6001 gatatcccat aaggactgcg gccagaagaa gcagaacgat tattaaaaaa caggttctcc
     6061 ttccggctgc cgaagtgcgg gccatcaggc tgccgcctgc tttaagcact cattgatccc
     6121 gttgataaag ccggatacat tgatgcttac agaagcaatg gcatctttgg catagccgtt
     6181 ttcatctgcc agctggctta ccgtctgatg ttcgatcaca taggcagcca gagcttccga
     6241 ctgggctttc catgtggggc cgtcctcggt catgatgtac agaccgtcca gagagagctg
     6301 ctgtttgcca atgccgtcct caccgaagca gtcccagctg caggcagtga tgatgccgtt
     6361 ttcaacggtc agctccacat ggttataaaa acccttggag tcaaattcgc tgtcatagga
     6421 atactggccg tcctttaaag gaaattccgg ctggtttaca gtaacaggtc cccgaagcag
     6481 ctggtaaata gatccagaag cagctataaa taccagagac agggctgcca taaggatgaa
     6541 gcctgccagg ttatgtttca tgaggtggtt ttgcttcata gtcagataca ctcctttttg
     6601 cgtggatgtt agattcccgt ttattttatc atacaaacag aacggttgta aaggaggaac
     6661 ttgctgtatg aatatacgaa cccgccgggt ggcggtttat ggacttttga ttgctcttgc
     6721 ctttgtattc ggctatattg aacgtattct tcccatctat ctgggagcgc cgggggtgaa
     6781 gctgggactt gcaaatctgg tgacgatcct gtccctttat gttttgggat tgtggccggc
     6841 agtatggatc agccttgccc ggatccttct ttccggcttt gcctttgggg gaatgtcctc
     6901 cattctgttc agcctggctg gagcggccct aagcctgctt gttatgtgga tctgccgccg
     6961 tttttctctt tttgatatgg tgggagtgag catactggga ggcgtatttc acaatatagg
     7021 ccagtttttg atggcggctt atgtggtcaa aacctttggc gtgttttctt atcttcccgt
     7081 gctgctgttt gcaggaaccg ctgccggggc agccataggg cttttaggcg gtctggtgtt
     7141 aaaacggatc gggaatgtga taaaaaatct gtaatctgat cggcattgca ttaaataagc
     7201 aaaaattgac tcattaaaac cgaaaaatcc ataaaaattg taatatgaca gaaaaaaatg
     7261 acattgtcat accgaagcgt tccttttata atagaggtat aaagtcaggc cggttttctg
     7321 gttaaaaaag ccgtcttttc aataaaaaat cacagggagg ttacagacat gatcagtaaa
     7381 gcagtcaaag atctggtaag ctatgggctg aaaacgggac ttctgaagga ggaggatgcg
     7441 gtttatgcca gaaacc
//
LOCUS       DBMDUH010000132         7360 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_4344_length_7360_cov_7.796666, whole genome shotgun sequence.
ACCESSION   DBMDUH010000132 DBMDUH010000000
VERSION     DBMDUH010000132.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 7360)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7360)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7360
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4344_length_7360_cov_7.796666"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(139..567)
                     /locus_tag="ACZPCG_10435"
     CDS             complement(139..567)
                     /locus_tag="ACZPCG_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HIR1285509.1"
                     /translation="MKVLKYSRQREAIKASLMNRCDHPTADALYASIREEFPNISLGT
                     VYRNLNLLVETGEIRKLSCGSGADHYDGDVSPHYHFVCRECGHVYDMDIQEMDELNAA
                     VQSHAPGHIDSHSILFFGQCQSCMDMENPKKDQKTIDKTA"
     gene            complement(666..1628)
                     /locus_tag="ACZPCG_10440"
     CDS             complement(666..1628)
                     /locus_tag="ACZPCG_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007719484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1285510.1"
                     /translation="MINFLNLEYFLAAAEELNFTRAAKRLYISQQSLSNHISNMEKEF
                     DVVLFNRTSPLTLTYAGQALKVRARELLDLKDETYREIADIKDFSTGQLSIGVSHTRG
                     RFILPEILPTYQSEFPGIELHLIEGNSSELANDLLHGTIDLMIDLLPFTAENVDTVPI
                     CQEEILLVVPDEVLKKAHPDGWDELRKKMEQGADVRLLENCPFVLLNKGNRVRTIADE
                     IFEDAQVTPHIVLETENIETALALSVKGMGITFYPRMFMSPDHAANTNKNPLTHQTGL
                     NLYPLNYSRAHGTLGLGYHKGHYMSRATHEFIRIALERIPYKND"
     gene            complement(1625..2416)
                     /locus_tag="ACZPCG_10445"
     CDS             complement(1625..2416)
                     /locus_tag="ACZPCG_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HIR1285511.1"
                     /translation="MIDLLDLHTHTIASGHAYNTLYEMAQSAAEKGLALYGCSEHAPA
                     MPGSCHKFYFSNFDVIPRVIRGIPVLMGTELNILDHTGRVDLEDHYLQKIDYAIASLH
                     TPCIDNHGTVSDYTNAYLGVMENPLIHIIGHPDDSRLPADWTAVAAAAAKHHKLLELN
                     SHSLAPNSSRKGARENYEQLLTACMRYGTSIIIDSDAHCENDVGNHEMAHRLLADLHF
                     PEELVVNTSLSKAAAFIPSLDRLLKEEPELFYNALSHPQGGNNRT"
     gene            2653..3414
                     /locus_tag="ACZPCG_10450"
     CDS             2653..3414
                     /locus_tag="ACZPCG_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HIR1285512.1"
                     /translation="MSEDMNKELETEVKAEEPVETMEDYAAELEASYAALDQRRDMPE
                     DDSAEGQKWAEFAQMMEDKTVLKVKISEAVKGGVVAFVDEVRAFIPASQLSADYVEKL
                     EDWQGKHVEAVIITVDPEKKRLVLSGRQVERQKREEARKERLAQFKPGDVVEGTVDSL
                     KDYGAFVKLAEGVDGLVHISQISTQRIKHPGAVLKEGQTVKVKILSIADGKISLSMKV
                     LAEQETAREEHETFNYKETASATTGLGDLLKGLKL"
     gene            3432..4079
                     /locus_tag="ACZPCG_10455"
     CDS             3432..4079
                     /locus_tag="ACZPCG_10455"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HIR1285513.1"
                     /translation="MINIPKTNEQIDQWRSVMFLYDSALKKMNTKIEILNNEFINRYD
                     YNPIEHIKSRLKTADSIVNKLKKDGREVSIENMQEYLSDIAGIRIICSFTSDIYQIAQ
                     IIAGQRDVTVLHVKDYIKYPKPNGYKSYHMVVTVPVYLSDGAVETKVEIQIRSVAMDF
                     WASLEHKIAYKFEGNAPENLLMELKDCADMVDMLDKKMFSLNEAIIAFAKNQQEE"
     gene            4226..4678
                     /gene="dtd"
                     /locus_tag="ACZPCG_10460"
     CDS             4226..4678
                     /gene="dtd"
                     /locus_tag="ACZPCG_10460"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HIR1285514.1"
                     /translation="MRAVVQRVTQASVTVDGELLGRIGKGLLILLGVADGDTRELTEK
                     MADKICRLRIFEDENGKTNLSLADVGGELLVISQFTLYADCRKGNRPSFVKAGAPQMA
                     EKLYEHFLARCREQADVVEKGQFGADMKVELLNDGPFTLMLDSEELFK"
     assembly_gap    4680..4779
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4780..5229
                     /locus_tag="ACZPCG_10465"
     CDS             <4780..5229
                     /locus_tag="ACZPCG_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002605627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HIR1285515.1"
                     /translation="RRKTVELVLYYGTNDPGMKKHEMMMKSVLVRMGVKIRNVKPEQV
                     METIGCLADMPGFQERKNVDEPLPVIPEQMLVMKDFTGSRIDTLLYQLRKAGVPKIDL
                     KAIVTEHNCGWSFYKLYEELKEEHRLMTEQARASQMEAGASDGEGSL"
     gene            5226..6668
                     /locus_tag="ACZPCG_10470"
     CDS             5226..6668
                     /locus_tag="ACZPCG_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002605303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR1285516.1"
                     /translation="MIDPVRERQLQELDTVGICTRILYQARSELYVNMHFLDVSLSSL
                     GFEADWGRNGISTDGAVIFYDPEFLLNLYKKGRVCVNRAYLHMVFHCLFCHMYTRKGR
                     KKELWDLACDIAMEAVIDGLYQKCVHVPQSPLRRETYLRLKRQMEEPEEKNKIQTERL
                     DGRDGIPKQGRTGSKVLTAERIYRALSRMELSERRLGQLQAEFKVDDHDLWEQDENSR
                     QAITRQNQWNDKREKMQTQMETMGSQDESQENESLQDMLQAENRERYDYRQFLRRFSV
                     LKEEIEVDPDSFDPVFYSYGMSLYGNMPLIEPLETKEVFRIEDFVIVIDTSMSCSGEL
                     VQRFLEETYDVLSESDTYFRKIRIHILQCDDKVQSDVLIECAEDLKAYMEHFQITGFG
                     GTDFRPAFEYVNELRAKREVDRLRGLIYFTDGKGIYPVQAPVYDAAFVFIEHLYSDES
                     VPAWAMKVVLEEEQLMEISGGDSKRKKDRE"
     gene            6665..>7360
                     /locus_tag="ACZPCG_10475"
     CDS             6665..>7360
                     /locus_tag="ACZPCG_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR1285517.1"
                     /translation="MNIKRAKEEIKDTAAAYLLKDDCGEYVIPSIRQRPVLLIGPPGV
                     GKTQIMEQIARECGLGLVAYTITHHTRQSAVGLPFIEHKVYGGKEYAVTEYTMSEIVA
                     SVYDRIRDTGLKEGILFIDEINCVSETLAPAMLQFLQCKTFGTHKIPEGWIIVAAGNP
                     PEYNKSVREFDVVTLDRIKKITVEPDLKVWKEYAYAQNLHPAVLSYLDIRPQYFCQVE
                     TTVDGKRFVTPRGW"
BASE COUNT         2002 a         1445 c         2011 g         1802 t
ORIGIN      
        1 ccgcaaactg tacaaaccca tttcttcata gtcttgatct cctttattat atagtgtgtg
       61 ggaaccgggg atgccccgga accctgttaa cattgcgaat caataatagt aatgattact
      121 gatatcaata taaatgattt atgctgtttt gtcaatagtt ttttgatctt tttttggatt
      181 ttccatatcc atacaggact ggcactggcc aaaaaacaga atggaatgag aatcaatatg
      241 tcccggcgca tggctctgga ctgctgcatt taattcatcc atttcctgaa tatccatatc
      301 atagacatgg ccgcattctc tgcatacaaa atggtagtgg ggcgatacat cgccgtcata
      361 gtggtctgct ccgcttccgc agcttaattt acggatctcg cccgtctcca ccagcagatt
      421 taagttccga taaacggttc ccaggctgat attgggaaat tcctcccgaa tgctggcgta
      481 tagggcgtct gccgttggat gatcgcaccg gttcatcaag ctggctttga tggcttccct
      541 ctgacggcta tactttaaaa ctttcatagg cgtctccttc taatataata atgataataa
      601 ttactgttta tattataagg agttctgctt ttgatgtcaa ggcaaaacag ataaaaacat
      661 cagcttcagt cattcttgta aggaatcctc tccaacgcaa tcctgataaa ctcatgggtt
      721 gccctggaca tatagtgtcc cttgtggtag cccagcccca gagtgccgtg ggcccgggag
      781 tagtttaatg gatagagatt cagccccgtc tgatgggtca ggggattttt gttggtattt
      841 gcggcatgat ccggggacat gaacatccgg ggataaaagg tgatccccat tcccttgacg
      901 ctgagtgcaa gtgccgtttc tatgttttcc gtctccagaa cgatatgggg agttacctga
      961 gcatcttcaa aaatttcatc tgcaatggtg cgcacccgat ttcccttatt taaaagtaca
     1021 aaggggcagt tttcaaggag acgtacatcg gcgccctgct ccatcttttt gcgaagctca
     1081 tcccacccat cgggatgggc ttttttcagg acttcatccg gaaccaccag aaggatctcc
     1141 tcctggcaga tgggtacggt gtctacattt tctgccgtaa agggaagcag atcgatcatc
     1201 aggtcgatgg ttccatggag gaggtcattt gccagttcgc ttgaattgcc ttcgatcaga
     1261 tgaagttcga tgcccggaaa ttcagactgg taggtaggga ggatttccgg caggataaaa
     1321 cggcctcttg tgtgcgaaac gccgatggaa agttggccgg tggagaaatc cttgatatca
     1381 gcgatttccc ggtaggtttc atcctttaaa tccaaaagtt ctctggcccg aacctttagg
     1441 gcctgccccg cataggtcaa agtcagcggg gaggtgcggt taaaaaggac aacgtcaaat
     1501 tccttttcca tattggaaat gtgattgctt aaggactgct gggaaatata gagccgtttg
     1561 gcggcccgcg taaaattcag ctcttcggca gctgccagaa agtattccag atttaaaaag
     1621 ttgatcatgt tcggttgttt cctccctgag gatgagataa tgcgttataa aaaagctccg
     1681 gttcttcctt taaaagccgg tccagagacg ggataaaggc tgcggctttt gagagagagg
     1741 tatttactac cagttcctcg ggaaaatgga gatcagctaa aagcctgtgg gccatttcgt
     1801 ggtttcccac atcattttca cagtgtgcgt cgctgtctat aatgatagag gtcccatagc
     1861 gcatacaggc agtcagcagc tgttcataat tttctctggc accttttctg gaagagttgg
     1921 gagcaagaga gtggctgttg agttccagga gtttatgatg ctttgcagcc gcagcagcta
     1981 ctgcggtcca gtcggcaggc aggcgggaat catcgggatg gccgatgatg tggatcagag
     2041 ggttctccat gacgcccaga taggcatttg tatagtcgga tacagtgccg tgattgtcga
     2101 tacagggggt gtggaggctg gcaatcgcgt aatctatttt ttgcaggtaa tgatcctcta
     2161 agtctactcg tcctgtatga tcaagaatgt tcagctcggt tcccattaaa accgggattc
     2221 cacggatcac tctggggatc acgtcaaaat tactgaaata gaatttgtgg cagcttccgg
     2281 gcatagcggg agcatgttcg gaacagccgt aaagggctag tcctttttcg gcagcggact
     2341 gggccatttc ataaagcgtg ttgtaggcat ggccgctggc gatggtatgt gtatgaagat
     2401 ctaaaagatc gatcatgaaa atatcctcct tatgaaataa atgcaagtgt tcttcctaaa
     2461 atatagcaca ggaaactttc cggtacaaag gaaaaaacag caaaaataaa aaacaggaat
     2521 aatttttctg aaaaagtata gaaatatcag gtggtttgct gatataatgt aaaaagctga
     2581 atatccagac agtctgaaag gatcaaaaca gactgagaca gggaatgaaa agcggaaggt
     2641 gaggagaaga acatgagcga agacatgaat aaggaattgg agacagaggt taaggcagag
     2701 gagcctgttg aaaccatgga ggattatgcg gctgaactgg aggcttccta tgcggctctg
     2761 gatcagagaa gagatatgcc ggaggacgac agcgctgaag gacagaagtg ggctgagttt
     2821 gctcagatga tggaggataa gaccgttctc aaggtaaaga tttccgaggc tgtaaagggc
     2881 ggggttgttg cctttgtaga tgaggtaaga gcatttatcc cggcgtctca gctgtcagcg
     2941 gattatgtag aaaagctgga ggactggcag ggcaagcatg tggaggctgt gatcattaca
     3001 gtagatccgg aaaagaagag actggttctg tctggccgtc aggtggaacg ccagaagagg
     3061 gaagaggccc gcaaagagcg tctggctcag tttaagccgg gtgatgtagt agagggcacc
     3121 gtagacagct taaaggatta cggcgctttt gtgaagctgg cagagggcgt agacggactt
     3181 gtgcatattt cccagatcag tacccagcgc atcaagcatc cgggagctgt tctcaaggaa
     3241 ggacagaccg taaaggtaaa gatcctttcc attgcagacg gaaagatcag cctgagcatg
     3301 aaggttctgg ccgagcagga gactgcgcgt gaagagcatg aaaccttcaa ctataaagag
     3361 accgcttcag ccaccacagg cctgggggat ctgttaaagg gactgaagct gtaggagagg
     3421 aagggatagg aatgatcaat atccccaaaa cgaacgaaca gatcgaccag tggcgttctg
     3481 tgatgtttct ctatgattca gccttaaaaa agatgaatac aaagattgaa atcctaaata
     3541 atgagttcat aaacaggtat gattacaatc cgattgagca tattaaatca aggctgaaga
     3601 cagcggacag tattgtaaat aaactgaaaa aggacgggcg tgaggtaagc attgaaaata
     3661 tgcaggagta tctgagcgat attgcgggta tccgcattat ctgctccttt acatctgata
     3721 tttatcagat cgctcagatt attgcggggc agagggatgt tacagttctt catgtaaagg
     3781 actatatcaa atatccaaag cccaacggat ataagagcta ccatatggtg gtaactgtgc
     3841 cggtttacct ttcagacgga gccgtggaaa caaaagtgga gatccagatc cgctccgttg
     3901 ccatggattt ctgggccagt ttagagcaca aaattgctta caaatttgaa ggaaacgctc
     3961 cggagaacct tctgatggaa ttaaaagact gtgcggatat ggtagatatg ctggataaaa
     4021 agatgttttc gttaaatgag gccatcatag cctttgcgaa aaaccagcag gaagaatagc
     4081 ccggagctcc gggagaaaaa taaaacctgc gccagccggc ggccttatgg aaccggctgg
     4141 ctctttcttt ttccggtgct ttgcaaaacg cgggagaagt gcaggagagg atgagaatgt
     4201 atcacacaca ggaggacgag agaaaatgag agcagttgtt cagagagtga cccaggcaag
     4261 tgttacagta gacggagaac ttttgggaag aattggaaaa ggccttctga ttcttctggg
     4321 cgtggctgat ggggatacca gagagctgac ggaaaagatg gcagacaaaa tctgccgcct
     4381 gcgtattttt gaggatgaga atggaaaaac caatttaagt ctggcggatg tgggaggaga
     4441 gcttcttgta atcagccagt ttacccttta tgcagactgc cgcaagggca accgccccag
     4501 ctttgtaaag gcaggagcac ctcagatggc ggaaaagctc tacgagcact ttctggcccg
     4561 gtgccgggaa caggcggacg tggtggaaaa agggcagttt ggcgctgata tgaaggtgga
     4621 gcttttaaat gacggtccct ttacgctgat gctggacagt gaggagctgt ttaaataaan
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna ggagaaagac agtggagctt
     4801 gtattatatt atgggacgaa tgatcccgga atgaaaaagc atgaaatgat gatgaagagc
     4861 gttctggtgc gtatgggtgt gaaaatccgc aatgtaaaac cggagcaggt tatggaaacc
     4921 ataggctgtc tggctgacat gccgggcttt caggagcgaa agaacgtgga tgagcctctt
     4981 cctgtgatcc cggaacagat gctggtgatg aaggatttta ccggcagccg gatcgacacc
     5041 ctgttatacc agttaagaaa agccggggtg cctaagatcg atttaaaagc cattgtgacg
     5101 gagcacaact gcggatggag cttttataag ctgtatgagg agcttaagga ggagcaccgg
     5161 ctgatgacag agcaggcccg ggcgtcgcag atggaggccg gagcgtctga tggagaaggt
     5221 tctttatgat tgatcccgta agagagagac agcttcagga actggatacg gttggaatct
     5281 gtacccggat cttgtatcag gccagaagcg agctgtatgt aaatatgcac tttctggatg
     5341 tgtccttaag cagcttgggt tttgaggcag attggggccg gaatggtata tccacagatg
     5401 gggcggtgat cttttatgat ccggaatttc ttttaaacct ctataaaaag ggccgtgtat
     5461 gcgtcaaccg tgcctatctg catatggtgt ttcactgcct gttctgccat atgtatacaa
     5521 gaaaggggcg aaagaaggag ctgtgggatc tggcctgcga tattgcgatg gaagcggtga
     5581 tcgacggcct gtaccagaaa tgcgtccatg tgcctcaaag ccctcttcgc cgcgaaacct
     5641 acctgcgcct gaaacgtcag atggaggagc cggaggagaa aaacaaaatt cagacagaaa
     5701 ggctggatgg cagagatggt attcccaaac agggaagaac cggcagcaag gttcttacgg
     5761 cagagcgcat ttaccgggcc ttaagccgga tggagctttc tgaacggaga ctgggacagc
     5821 ttcaggcaga atttaaggtg gacgaccatg atctctggga acaggatgag aattcccgtc
     5881 aggccattac acgccagaac cagtggaatg ataaacggga aaaaatgcag actcagatgg
     5941 aaactatggg ttctcaggat gagtcacagg aaaatgaaag tcttcaggat atgcttcagg
     6001 cagaaaacag ggagcggtac gattaccgtc agtttctccg gcgtttttct gtgcttaaag
     6061 aggaaattga ggtggatccg gactcttttg atccggtttt ttattcctat ggaatgtccc
     6121 tttatggaaa tatgcctttg atcgagcctc tggaaacaaa agaggtattt cggattgagg
     6181 attttgtgat tgttattgac accagtatgt cctgttccgg ggaactggtt cagaggtttt
     6241 tagaggagac atatgatgtg ctttctgagt cagatactta tttcagaaag atacggattc
     6301 atattttgca gtgtgacgat aaggtgcagt cggatgtgct gattgaatgt gcagaggatc
     6361 taaaggctta tatggagcat tttcagatca ctggctttgg aggaacggat ttccgcccgg
     6421 cctttgaata tgtaaatgag cttcgggcca aaagagaggt agacaggctt cggggactga
     6481 tctactttac ggatggaaaa ggaatctatc cggttcaggc tccggtttat gatgctgcct
     6541 ttgtatttat tgaacattta tattccgatg aatcagtgcc tgcctgggcg atgaaggtgg
     6601 ttctggagga ggagcagctt atggagattt cggggggaga ttcgaagaga aagaaggaca
     6661 gagagtgaat attaaacgag ccaaggaaga gataaaggat accgcagcag cctatctttt
     6721 aaaggatgac tgcggagaat atgtgatccc ctccatccgc cagcggccgg tgcttttgat
     6781 cggcccgccg ggagtgggaa aaacccagat tatggagcag attgccagag aatgcggttt
     6841 gggtctggtt gcctatacca ttactcacca tacaaggcag agcgccgtgg gtcttccgtt
     6901 tattgaacat aaggtttacg gaggaaagga atatgcggtg acggagtaca caatgagcga
     6961 aattgtggct tcggtgtatg acaggatccg cgatacaggg cttaaggaag ggatcctttt
     7021 tatcgacgag atcaactgcg tatcggagac attggctcct gccatgctgc agtttcttca
     7081 gtgcaaaacc ttcggaaccc ataagatacc ggagggatgg atcatcgtgg cagcgggaaa
     7141 cccgccggag tacaataaat ctgtccggga atttgacgtg gtaacactgg atcgaatcaa
     7201 aaaaattacc gtagagccgg atctgaaggt atggaaggaa tatgcctatg cgcagaatct
     7261 ccatccggcc gttttatcct atctggacat ccgtccccag tatttctgcc aggtagagac
     7321 caccgtggat ggaaagcggt ttgttacgcc cagaggctgg
//
LOCUS       DBMDUH010000133         7318 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_4374_length_7318_cov_7.124428, whole genome shotgun sequence.
ACCESSION   DBMDUH010000133 DBMDUH010000000
VERSION     DBMDUH010000133.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 7318)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7318)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7318
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4374_length_7318_cov_7.124428"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1871)
                     /locus_tag="ACZPCG_10480"
     CDS             complement(<1..1871)
                     /locus_tag="ACZPCG_10480"
                     /inference="COORDINATES: protein motif:HMM:NF016330.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HIR1285518.1"
                     /translation="MTDWKTILAGADDEYLASLSNKGTVKRAHKDRETTPCEILDTGD
                     QGINVKLGEEELLIAPVLAESRCSCPSRSICRHIVQAILVLKDMYEAGEGGIKEEKKE
                     KSEALPSEELWQAVLEIGNQKIIRALGMKKLGEVLGQIRAGKRADIARSSTVLVELPD
                     SELQVKFLLPLEYSSCTCKKKEFCAHRAAAALWCRMEAGTLCLEEFEKEEEEPKADLN
                     RIREKAGEMKAALEDILASGLSRTASQSADTLERLAVISHNEGLASFEDAFRSLQNGY
                     EAYFSRSSSVNAAGLMDESARLYEQICRLSRVKTDGELRNLGGVFHMDYEPAGDMELI
                     GITAEAFQSRNGYQGETVYFLEKNTKKWYTYTSARPVYYDSRGRRGRMEKAAAPWGLN
                     VSLEELAGTEVCLKQAKAGKTGRLSSSQDTKGMITGKHSFCPEDAGKWLYKDFGALFK
                     EQCFLKNSWLKREEETLRIVCVQAAAWDAARFSQAEQKFSMAVFDSNGKELLTEMAYS
                     RREDANIRYLERIADQVEKGEIPIPAIAGKLYLKDGKMILHPLEIFQWGQEAGKREAF
                     ADEWVSDSPQERERVLKINAMEQMYRLLEEVSQQIEDLYQSGFDAVHDSTLGMLRQWA
                     "
     gene            complement(1868..2995)
                     /locus_tag="ACZPCG_10485"
     CDS             complement(1868..2995)
                     /locus_tag="ACZPCG_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR1285519.1"
                     /translation="MRDEKEILNRWRLALGRYAEGPVHFSEGSLPYGEMDQVLEFLYS
                     REYGEEQEIRKDRGGSGDPSSLTVPSWIEKLRRLFPKSTAEIMERHALEKYNMTELFT
                     DPEVLKRMEPNRELLKMVLELKSMMKGPVLEMAREVVKKVAEELTKELEQEIRPALTG
                     RMDRFSGSPVRSMRNLDIKKTIRMNLKHYDVEKRQLLLDQVYFYGRLKRHSPWNVIIC
                     VDESGSMMDSVIHSAVMAGIFARLPVMDVKLVIFDTSVVDLSGYVEDPVETLMSIQLG
                     GGTDIGGALRYCETLIEAPARTIVVLVSDLYEGVSQANMYGAVKDIVESGARLVALTA
                     LDRQADPVYDRNAAEKMARLGAHVGAMTPEQLAEWMGKIIA"
     gene            complement(2985..5324)
                     /locus_tag="ACZPCG_10490"
     CDS             complement(2985..5324)
                     /locus_tag="ACZPCG_10490"
                     /inference="COORDINATES: protein motif:HMM:NF040143.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HIR1285520.1"
                     /translation="MAEIILLPVRHHSPACAWHIVRMIEKLKPDAVLIEGPENAGSLI
                     PAMIHEETKAPFAVYYSYQDQAGEIGGGEEYYKCYYPFLDYSPELAALRTCRDLGIPG
                     NFMDLPYREILAACEKSRSEGLTGDRLLSDGRFFQKLCQKTGLRSFDEFWEKYFEIQG
                     LCMESETWFEMLLGYCRMIREDTPPEQICSEGCEAREQFMAGRLKKKAAEVGEEGLVL
                     GITGGFHTPALAEYLREQTKLKEWKEQAKKGEEGIYLMPYSMEETDAWGGYASGMPFP
                     GFYQRIWEKLEENKEKEQPQKGVYEGAVLDFLIETGRDGRKKDGVPTAYDEICALDQA
                     RGLASLRDKREPGAWELKDAVLSSFIKGECSLSSDKPLRILKKHMTGTRLGKLCKQAE
                     VPPLIQDFERQCARFGIRSRSAMEIKRVLTPFSNEKHREESKFLNRMVFLQTEFARKT
                     KGPDLRLGRDRNMMRETWICRFRPSVAAALMDVSVRGAVIEEAVTSLVREELKTESDA
                     GKAALLLTSVFEMGLDQEMEPVYEVVSRIILEDTRFFAVAEALSRLRMLKELQGLYRV
                     NLPFERLIAGCYEKLVILLPSMARIKDEDLESAMKAMKLLYQTGGQTGCSREAYFEAL
                     ERMREDGKLHPGLEGCIHGILFGCGREEAYEAEAAGRGYITGTREQLLKTAVFLRGLF
                     FTARDLIFMGQGMIPMLDAFFSQVEDGEFLELLPQLRLAFGAFTPGELKRVGNLAAGL
                     HREKSLEKETPPVLPGVFAYGKELENFVKLSMEGEPDER"
     gene            complement(5312..6427)
                     /locus_tag="ACZPCG_10495"
     CDS             complement(5312..6427)
                     /locus_tag="ACZPCG_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1285521.1"
                     /translation="MTEQQWIKPPVEIRYAEELKALKENDTGPRPLNWQLSPKAVRTF
                     ILGSSKEIPYSGGACRIRKKYFGNDALVERSIVTLAGNRGLMLVGEPGTAKTMLSELL
                     SAAISGVSTNTIQGTAGTTEDMIKYSWNYALLLAKGPVREALVPSPLYTGMEKGILTR
                     FEEITRTPAEIQDSLISVLSDKLLNIPELGDEGFLFAAPGFNVIGTANTRDKGVNEMS
                     SALKRRFNFETVQPVREVSMEKQIILNEVESLAKESHMEMELDESAAELLASTYHELR
                     EGVSSLGHRIDRPGAVMSTAEAVSVYYQTMISGYYYGNKTMDIDCLVQNLTGAISKEN
                     RDDLEKVKAYFGTVIRDKSSREGGIWSRYYDARKWLK"
     gene            6524..>7318
                     /locus_tag="ACZPCG_10500"
     CDS             6524..>7318
                     /locus_tag="ACZPCG_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIR1285522.1"
                     /translation="MGFVDLHTHSDASDGSFTPAHLVHLAKEAGLCAIALTDHDTTAG
                     IADAVDAGKELGVEVIPGIEVSGNYEGHEIHILGLFINPDDPELKAFLKDMRSRRNRR
                     NEEMFRRLAADGISFTDEELFGSNPQTVITRAHVARAMVQRGFCSSANQAFKKYLEYG
                     GRYCPPKEETEPESIVKILLKNGAFAALAHPFLYKLGDKQTKALITRLAEAGMQGLEV
                     YHSSNYPLESRKLQKMAAGLKLLPTGGSDFHGSCKPDISIGTGRGGL"
BASE COUNT         1638 a         2211 c         1558 g         1911 t
ORIGIN      
        1 gcccattgcc gaagcatccc aagggtgctg tcatgaacgg cgtcaaaccc gctctggtac
       61 agatcctcga tctgctggct gacctcttcc agaaggcggt acatctgctc catggcattg
      121 attttaagta cccgttcccg ttcctgcggt gaatctgaaa cccattcatc tgcaaacgct
      181 tcccgtttgc cggcttcctg cccccactga aaaatctcca gaggatgaag gatcattttc
      241 ccatctttta agtacagctt gcctgcaatg gcaggaatgg gaatttctcc cttttccacc
      301 tgatctgcaa tccgctccag atagcggata ttcgcgtcct cccgcctgct gtatgccatc
      361 tctgtcaaaa gttctttccc attggaatca aaaacagcca tggaaaattt ctgctctgcc
      421 tgagaaaaac gggccgcatc ccaggctgca gcctgaacgc acacgatccg cagcgtctcc
      481 tcctcccgtt tcaaccagct gtttttcaaa aaacactgtt ctttaaaaag cgctccaaag
      541 tctttataaa gccattttcc cgcgtcctcc gggcagaagc tgtgttttcc tgttatcatg
      601 ccttttgtat cctgactgga tgacagacgg cctgtttttc cggcttttgc ctgttttaag
      661 cagacctctg ttccggccag ttcctctaag gatacattta atccccaggg agcggctgcc
      721 ttttccatcc gcccccgtct tccccggctg tcatagtaca ccggacgggc gctggtatat
      781 gtataccatt tctttgtatt cttttccaga aaatataccg tttctccctg ataaccgttt
      841 ctgctctgaa aggcctccgc cgtaatgccg atcagttcca tatctcctgc cggttcatag
      901 tccatgtgaa agactcctcc caggttccta agctccccgt ctgttttcac ccggctcaaa
      961 cggcagatct gttcataaag gcgggcgctt tcatccatca gccctgcggc atttacagag
     1021 gatgaacggc taaaatacgc ttcatatcca ttttgaaggc tccggaacgc atcttcaaag
     1081 gaggccagtc cttcattgtg gctgatcact gccagccgtt ctaaggtatc ggcactctga
     1141 ctggcagtgc gcgaaaggcc ggaagctaat atatcctcta atgcagcctt catctcaccg
     1201 gccttttccc gtatccggtt cagatccgct ttcggttctt cctcttcctt ttcaaattcc
     1261 tccaaacaca gagttcccgc ctccatacgg caccacaaag ccgcagcagc cctgtgagcg
     1321 caaaattcct tctttttgca ggtacaggag gaatattcca gcggaagcag aaattttacc
     1381 tgcaactctg aatccggaag ctccaccaaa accgtgctgg aacgagcgat atctgccctt
     1441 ttccccgccc ggatctgccc cagtacctcc ccaagcttct tcatccctaa tgcccgtatg
     1501 atcttctggt tcccaatctc cagaacggcc tgccaaagct cctctgaggg cagagcttcg
     1561 gatttttctt tcttctcctc ttttatcccg ccttctcccg cttcatacat atccttaaga
     1621 accaaaatag cctgcacaat atgcctgcat atggatctgg acggacagct gcaccggctt
     1681 tccgccagca ccggagcgat cagaagctct tcctctccaa gctttacatt tatgccctga
     1741 tcccctgtat ccaaaatctc acagggagta gtttctctgt ccttatgggc ccgctttacc
     1801 gtacccttat tgctgagact ggccagatat tcgtcatccg caccggccag tatggttttc
     1861 caatctgtca tgctatgatt ttccccatcc attctgccag ctgctccgga gtcatagccc
     1921 ctacatgagc ccccagccgc gccatctttt ctgctgcatt tctgtcatat accggatctg
     1981 cctgacgatc cagagctgta agagctacca gtctggctcc gctttccaca atatccttca
     2041 cagcgccgta catatttgcc tggcttacac cctcataaag atcggatacc agcaccacaa
     2101 tggttctggc cggagcttcg atcagggttt cacaatacct taaagctcct ccgatgtccg
     2161 tgccgccgcc cagctggata ctcataagcg tttcgaccgg gtcttccaca tatccgctta
     2221 aatccaccac actggtatca aaaataacca gcttcacatc catcaccgga agcctggcaa
     2281 aaatccccgc catgacagca ctgtggatca cgctgtccat catggagccg ctttcatcaa
     2341 cacagatgat tacattccat gggctgtggc gtttcagcct gccgtaaaaa tagacctgat
     2401 ccagcagaag ctgcctcttt tccacatcat aatgctttaa attcatccga atggtcttct
     2461 ttatatccag attccgcata gagcgcaccg gactgcccga aaaacgatcc atgcggcctg
     2521 taagcgcagg ccgtatctcc tgctccagct cctttgtaag ctcttctgcg acctttttta
     2581 ccacctcacg ggccatttca agaaccggcc ccttcatcat ggatttcagt tccagcacca
     2641 tttttaacag ctccctgttg ggctccatgc gctttaatac ctcaggatcc gtaaaaagct
     2701 ccgtcatatt gtacttttcc agagcatggc gctccataat ctctgcggtt gacttgggaa
     2761 acaggcggcg cagtttctca atccaggagg gtacggtaag gctggaggga tcgccgcttc
     2821 ccccgcggtc ctttcggatc tcctgttctt ccccgtactc tctggaataa aggaactcca
     2881 gcacctgatc catttcacca tagggcagac tgccctctga aaaatgcacg ggcccttcgg
     2941 cataccggcc cagagccaga cgccagcggt tcagtatctc tttttcatct ctcatccggc
     3001 tctccctcca tggatagttt tacaaagttt tccagttctt tcccataggc aaacacaccc
     3061 ggaagtactg gcggcgtttc tttttccaga cttttttccc ggtgaagccc ggccgccaga
     3121 tttccaaccc tctttaactc tcccggagta aatgcgccaa aggccagacg aagctggggc
     3181 agaagttcta aaaattcccc atcctccacc tgggagaaaa acgcatccag catgggaatc
     3241 atgccctgcc ccataaaaat gagatctcta gctgtgaaaa aaaggcctct gagaaaaaca
     3301 gccgttttaa gaagctgctc tctggttccg gtaatgtatc cccggcccgc agcctccgct
     3361 tcatacgcct cttccctgcc gcagccaaac aggatcccgt gaatacatcc ctccaggccc
     3421 ggatggagct ttccatcctc ccgcatccgc tctaaagcct caaaatatgc ctccctgctg
     3481 cagcctgttt gccctccggt ctgatagaga agcttcatgg ccttcatggc gctttccaga
     3541 tcctcatcct taatccgggc catggaggga agaaggatca ccagcttttc ataacagcct
     3601 gcgatcagcc gttcaaaagg aagatttacc cggtaaagcc cctgaagctc tttcagcatc
     3661 cgcaggcgtg acaatgcctc tgccactgca aaaaaccggg tatcctccag aatgatccgg
     3721 ctgaccactt cataaacagg ctccatctcc tgatccagtc ccatttcaaa cacagaggta
     3781 agcaaaaggg cggctttccc ggcatcactc tccgtcttaa gttcctctct tacaaggctt
     3841 gtcactgcct cctcaatcac tgccccccgg acggatacgt ccataagggc tgctgctacg
     3901 gagggtctga agcggcagat ccaggtttcc cgcatcatat tccggtctct tcccagacgc
     3961 agatccggtc cctttgtctt tctggcaaac tctgtctgaa gaaataccat cctgtttaaa
     4021 aatttgctct cttccctgtg tttttcgttg gaaaatgggg tgagtacccg cttgatctcc
     4081 atagcagacc ggcttcggat gccgaacctg gcgcactgcc tttcaaaatc ctgaatcaga
     4141 ggcggtacct cagcctgctt gcagagcttt cccaaccggg ttcctgtcat atgctttttg
     4201 agaatccgaa gtggcttatc cgatgataag gagcactcgc ccttaataaa ggaggaaagc
     4261 accgcatcct ttaattccca ggctccgggc tctctcttat cccgaaggga tgcaagccct
     4321 cttgcctgat ccagagcaca gatctcatca taggcggtgg gaacgccgtc cttttttctc
     4381 ccgtcccttc cggtctcaat caggaaatca aggactgctc cttcatacac tcccttctgc
     4441 ggctgttcct tctctttatt ttcttccagt ttttcccata ttctctggta aaagccggga
     4501 aaaggcatac cgctggcata accgccccag gcgtctgtct cctccatgga atagggcatc
     4561 aggtaaatgc cctcctcccc cttttttgcc tgttccttcc attctttaag ctttgtctgt
     4621 tccctgagat actctgccag cgccggagta tgaaatccgc ctgtgattcc aagcaccagc
     4681 ccttcttctc ccacctcggc cgcctttttc ttaagccttc ctgccataaa ctgctctctg
     4741 gcctcgcagc cctctgaaca gatctgctcc ggcggggtat cctcccggat catccggcag
     4801 tagccaagaa gcatttcaaa ccaggtttcg ctctccatgc acaggccctg gatttcaaag
     4861 tacttctccc aaaattcatc aaaggagcgc agaccggttt tctggcagag cttctgaaaa
     4921 aaacggccgt ccgacagcag ccgatcccct gtaaggcctt cgctgcggct cttttcacag
     4981 gcagccagaa tctccctgta aggcagatcc ataaaattac ctgggattcc cagatctctg
     5041 cacgtcctca gggctgccag ctccggcgaa taatccagaa agggataata acacttatag
     5101 tattcttcac cgccgccaat ctctcctgcc tgatcctgat aggaataata tacggcaaag
     5161 ggcgccttgg tctcctcatg gatcatggcc gggatcaggg agcctgcatt ttcaggccct
     5221 tcgatcagaa ccgcatcagg ctttaatttt tctatcatcc gcacaatatg ccaggcgcag
     5281 gccgggctgt gatggcgcac gggaagaagg attatttcag ccatttcctt gcgtcgtagt
     5341 atctgctcca tattccgccc tctctgctgc tcttgtcacg gatcactgtt ccaaaatagg
     5401 cctttacctt ttccagatca tccctgtttt cctttgaaat tgctccggta agattttgta
     5461 ccaggcagtc aatatccatg gttttatttc catagtaata acctgaaatc atggtctgat
     5521 agtacacaga tacagcctct gccgtactca taaccgctcc cggccggtca atccggtgtc
     5581 ccagggaaga tactccctcc cgcagttcat ggtaggtaga agccagcagc tctgccgcac
     5641 tctcatccag ctccatttcc atatggcttt cttttgccag gctttccacc tcatttagaa
     5701 tgatctgctt ttccatggaa acctcacgca ccggctgaac cgtttcaaag ttaaaacggc
     5761 gttttaaggc gctgctcatc tcatttacgc ccttatcccg tgtattggcc gttccgatta
     5821 cattaaatcc gggagcggca aagagaaaac cctcatcgcc cagctccgga atatttaaaa
     5881 gcttatcgct taaaacgctg atcaggctgt cctgaatctc tgccggagta cgggtgatct
     5941 cctcaaagcg ggtaaggatt cccttttcca tgcccgtata caaaggcgac ggcaccagag
     6001 cctcgcgcac cggccccttt gccagcagca gcgcataatt ccatgaatac ttgatcatat
     6061 cttctgtggt tccggctgtt ccctggatcg tattggtgct gacgccgctg atagccgcag
     6121 agagaagctc cgacagcatg gttttggccg ttcccggctc tcccaccagc atcagccccc
     6181 tgtttcccgc cagcgtaacg atgctgcgtt ccaccagagc atcatttcca aaatatttct
     6241 tccggatccg gcacgcgccg cccgaatagg gaatctcctt tgaacttccc agaataaagg
     6301 tgcgcacagc ctttggtgaa agctgccagt ttaggggtct ggggcctgta tcattttctt
     6361 ttaatgcctt tagttcctct gcataccgga tttccaccgg cggtttgatc cactgctgct
     6421 ccgtcatctg tatgtcctcc tttatatcag gaaaatatat atcagtatag caaaattccg
     6481 tctgctgtgc tactatatat acaaaaaaag gaagtgaata tccatgggtt ttgttgattt
     6541 gcatacacac tctgatgcat cagacggcag cttcactccg gcccatcttg tgcatctggc
     6601 aaaggaggcc ggtctttgcg ccatcgccct taccgaccat gatacaactg ccgggattgc
     6661 cgatgccgtt gatgccggaa aagagctggg agtagaggtt atccccggca ttgaagtatc
     6721 cggtaattac gaaggccatg aaatccatat tttaggactt tttatcaatc cggacgatcc
     6781 ggagcttaaa gcatttctca aagacatgag aagccgccgg aaccggcgca atgaggagat
     6841 gttccgccgt ctggcagcag acggaatctc atttacagat gaagagctgt tcggttccaa
     6901 tccccagacc gttatcacaa gagcccatgt ggcccgtgcc atggttcaga gggggttctg
     6961 ttcttctgcg aatcaggcct ttaaaaaata tctggaatac ggtggccgct actgcccgcc
     7021 caaggaggag accgagccgg aatccattgt aaaaatcctg cttaaaaacg gggcttttgc
     7081 agctttggcc catccctttt tatacaagct cggagacaaa cagacgaaag ccctcattac
     7141 ccggctggct gaggccggaa tgcagggatt agaggtctac cattcttcta attaccctct
     7201 ggaaagccgc aagctgcaga aaatggctgc cggccttaag cttcttccta caggaggctc
     7261 tgatttccac ggctcatgca agccggatat ttcaatcggc acaggccggg gcggactg
//
LOCUS       DBMDUH010000134         7279 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_4399_length_7279_cov_4.642240, whole genome shotgun sequence.
ACCESSION   DBMDUH010000134 DBMDUH010000000
VERSION     DBMDUH010000134.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 7279)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7279)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7279
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4399_length_7279_cov_4.642240"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="ACZPCG_10505"
     CDS             <1..711
                     /locus_tag="ACZPCG_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIR1285523.1"
                     /translation="ITIQSSLSSENREQIDRMAAELCEEYIARSQVTANKHDMKALFK
                     IGYGLYVVTSNDGTRDNGLIVNTVTQLTDTPLRVAVNINKANYSHHVIKQTGIMNVNC
                     LSVEAPFKIFETFGFQSGRQADKFAGMKICRSDNGLAFLPKYINAFMSLKVDQYVDLD
                     THGMFICSVTEARVMSDRETMTYTYYQNNVKPKPQTDGKKGFVCKVCGYIYEGDTLPD
                     DIICPLCKHGAADFEPIE"
     gene            801..1916
                     /locus_tag="ACZPCG_10510"
     CDS             801..1916
                     /locus_tag="ACZPCG_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR1285524.1"
                     /translation="MEKIRMAVIGIGTMGKKYAKMIQEGRISGMCLGAVCCRSHESSQ
                     WAKAELGGELPVFQDTEKLFDASELFDACLIVTPHKLHPEMIIQAFDKGKHIFCDKPI
                     GVSMGQCLKIRQKAEEKDLVFAMMFHQRMYPKYERIHEFLESGTLGQIRRILMENSRY
                     YRTWHYHKSGSWRSSWRGEGGGALLNQGQHLLDIWQWLFGMPESLYALIPYGKYNDFS
                     VDDEATIVMKYPDKKTAVFILTTGEGTWTERLEIVGSKGTLLLEDSHLTLHLYDRDLE
                     EYKRTASCNSREELKERVTEETFPQDEEPYEEMLADFAQAVLEKRPVRVNGLEGLNSL
                     ELTNAAYLSAWMGAPVSLPIDEVQYERELEKRIREEI"
     gene            1913..2974
                     /locus_tag="ACZPCG_10515"
     CDS             1913..2974
                     /locus_tag="ACZPCG_10515"
                     /inference="COORDINATES: protein motif:HMM:NF013567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR1285525.1"
                     /translation="MRPLSFVIIGSGFRAMFYGRIARRYPELFTLKYILCRSEEKAAK
                     VHGETGFPTTVSDEDCKNSKPDFVVVAVNKGNIADVAEKWALMGYPVLTETPIGCSLK
                     QLRRIWELKEKYHCKIQVCEQYFHCPTLRAGLHQIQNEAIGQPDSMYLSLVHEYHGAS
                     LIRKYLLMGHERFSVIGKQFTFPLTETDSRQGAITDGSVSSRGRIMALFEFASGKTAL
                     YDFSGEQYRSFIRSRHVTVRGQKGELTDQILYRLTEENFPETLYIMPELDSRYRQLET
                     KRLRDCVRGWKPELYLDEIEDEYAIACMLYDMREYLDSGKEIYPLRDALQDSYMALLL
                     REAVMSEGCEIHSQPMPWN"
     gene            3088..5025
                     /locus_tag="ACZPCG_10520"
     CDS             3088..5025
                     /locus_tag="ACZPCG_10520"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016439890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HIR1285526.1"
                     /translation="MKKGKMWKAGAAGIMAAGTGAVLMMASRKKGRMHRLTDSNSSCK
                     NKGSKGENQRIYYSKGNYEAFARPEKPEGIEEKSAWLIGGGLASLAAACFLIRDAGMS
                     GDKIHILEASDRTGGACDGILDSSRGYIMRGGREMENHFECLWDLFRSIPSLDTPGAS
                     VLDEFYWLNKADPNYSLCRVTEKQGKPACTQGYFGLSEKAGRQIMRLFFTCDEDLYDK
                     TIEDVFDNEVFDSPFWLYWRTMFAFENWHSALEMKLYLRRFVHHIGGLPDFSALKFTR
                     YNQYESLILPLKRYLEEAGVEFRFGVAVTNVEFDIREGKKTAKFLDCMINGAKAQIPL
                     TEQDLVFITNGSCTEGTVYGDQDHKPDPKRQSEAEHCWTLWKNIAVQDTSFGRPEKFC
                     SNREKTCWESATITTSDETILSYIKKIARRDPRTGKVVTGGIVSCQDSGWLLSWTVNR
                     QGQFKAQKKEEVCIWLYGLFPNGVGNFVPKPMSECTGREITEEWLYHLGVPADQIHDL
                     AAWGAVCVPAMMPYITAFFMPRREGDRPDVIPEGSVNAAFIGQFAQTPRDTVFTTEYS
                     VRTAMEAVYGLCGVDRGVPEVWGSAYDMRELLNASVKLMDGVSPLKKRLPACFEKAGK
                     MAIKKIEHTEIGELLRECGVL"
     gene            complement(5105..5968)
                     /locus_tag="ACZPCG_10525"
     CDS             complement(5105..5968)
                     /locus_tag="ACZPCG_10525"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005341341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HIR1285527.1"
                     /translation="MKPFKEGKVREIYDNGDSLIMVATDRISCFDVILHNVVAGKGTV
                     LTQMSRFWFDFTKDILPNHMISVDVKDMPEFFQQPEFEGKSMMCKKLTMIPVECIVRG
                     YITGSGWKSYQENGTVCGIHLLEGLQESEKLPEPIYTPSTKAEIGDHDENISFEQSVS
                     HLEKYFPGKGEHYATLIRDYTIALYKKCADYALSRGIIIADTKFEFGLDEDGNVVLGD
                     EMLTPDSSRFWPAQGYEPGHSQPSFDKQFARDWLTAHPGNDWTLPEEIVEKTIDKYLQ
                     SYEMLTGTKLQ"
     gene            6231..>7279
                     /locus_tag="ACZPCG_10530"
     CDS             6231..>7279
                     /locus_tag="ACZPCG_10530"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014026.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HIR1285528.1"
                     /translation="MIRQKKTHSKKKGAIRPGLVITSLLLAVSIGVTGTAVYFKDIVN
                     LYLAADRTRVSEEEMEKTAQESQLLSHRIQEEGSVLLKNTKDTLPLSDSVRQVNVFGW
                     ASTQWLGGGSGSGRITQVKTDFLAALRDAGVSYNEELIGMYREFQPKRPYSDTLNSWP
                     QESCRLYEPHISDREYYTEELLDHARDYSDTAIAVIGRFAGESNDCPKVQYKQTKKGG
                     EITEDPTRTYLDLSKEEEDLLRYLGVSYKNVILVLNTGNVMALGAIERLSGIDACLYA
                     GLTGESGAGVLPKILWGRVSPSGRTTDTWAYDFSTAASYVNAGGEGVGRYTNAETLYP
                     ADGTKCGNLGENFAY"
BASE COUNT         2052 a         1474 c         1932 g         1821 t
ORIGIN      
        1 attaccattc agtcctcact gagcagtgag aacagagagc agattgacag aatggctgct
       61 gaactctgtg aggagtatat tgcccgttct caggtcacgg caaataagca tgatatgaag
      121 gctctcttta agatcggcta tggtctttat gtagtgacct caaatgacgg aacaagggat
      181 aacggcctca ttgtaaatac ggtaacgcag ctaacagata cgcctctgcg ggtagctgtg
      241 aatattaaca aggccaatta ttcccatcat gtgatcaagc agaccggtat tatgaatgta
      301 aactgcctgt ccgtggaagc tcccttcaag atatttgaaa cctttggatt ccaaagcgga
      361 cgtcaggcag ataaatttgc aggtatgaag atctgccgtt cggacaatgg cctggctttc
      421 ctgcccaagt atatcaatgc ctttatgtca ttaaaggtag atcagtatgt ggatctggat
      481 actcacggta tgtttatctg ctccgtaaca gaagcaaggg tgatgagcga tcgggagacc
      541 atgacctaca cctattatca aaacaatgta aagccgaagc ctcagacaga tggcaagaag
      601 ggctttgtgt gcaaggtatg cggctatatc tatgagggag atactctgcc ggatgatatt
      661 atctgcccgt tgtgcaagca tggcgcagct gattttgaac ccattgagta ggctgcagaa
      721 taactaaatg atgaatcagg cgggccggga aacgttttcc ggcccgcctg ctgcttaaaa
      781 cagaaaggaa gggaagacgg atggaaaaaa tcagaatggc cgttattggg atcggtacaa
      841 tgggaaaaaa atatgcgaaa atgatacagg agggaaggat ttccgggatg tgtcttgggg
      901 cagtctgctg caggagccat gaaagcagtc agtgggcaaa agcggagctt ggcggagagc
      961 ttcctgtctt tcaggacaca gagaagttgt ttgatgcctc agagctgttt gacgcctgtc
     1021 ttatagttac gccccataag ctgcatccgg agatgattat acaggcattt gataagggaa
     1081 agcatatatt ctgtgataag cccataggcg tttccatggg gcagtgcctg aagatccgtc
     1141 agaaggcaga agagaaggat cttgtttttg caatgatgtt tcatcagagg atgtatccaa
     1201 agtacgaacg gatccatgag tttcttgaaa gtgggactct gggacagatc cgccgcattc
     1261 tgatggagaa ctccagatat tacagaacct ggcattatca taaatccgga agctggcgaa
     1321 gcagctggag gggagaagga ggcggtgcac ttttaaacca gggacagcac ctgcttgata
     1381 tctggcagtg gctttttgga atgccggaaa gcctttacgc tttgattccc tatggaaaat
     1441 ataacgattt ttcagtggat gatgaggcta cgattgtgat gaaatatccg gataagaaaa
     1501 cagcagtatt tattcttact acgggagaag gaacctggac cgaacggctg gaaattgtgg
     1561 gatctaaggg aacgcttctt ttggaggaca gccatctgac ccttcatctg tatgatcggg
     1621 atctggaaga atataaaagg acggcttcct gcaattccag agaagaactg aaggagagag
     1681 ttacagagga aacatttcct caggatgaag aaccctatga ggaaatgctg gcggattttg
     1741 cacaggctgt tttggaaaaa cgtccggtca gagtcaatgg gctggaaggc ttaaattctc
     1801 tggagcttac caatgccgcc tatctttctg cctggatggg agcgcctgtt tctcttccga
     1861 tagacgaagt gcagtatgag agggagctgg aaaaacggat cagggaggaa atatgagacc
     1921 attgtcattt gttatcatcg gctccggctt cagagccatg ttttacggga gaattgccag
     1981 acggtatccg gagctgttta cactaaaata catattatgc agatctgagg agaaggctgc
     2041 aaaggttcat ggggaaacag gctttcccac aactgtatca gacgaggact gcaaaaactc
     2101 caaaccggat ttcgtggttg tggcagtaaa taaagggaat attgcagatg tggcagagaa
     2161 atgggcgttg atgggctatc ctgtgcttac tgaaacaccg attggatgca gtctgaaaca
     2221 gttaagacga atctgggaat taaaagagaa ataccattgt aaaattcagg tctgcgagca
     2281 gtattttcat tgtcccacac tcagggcagg actgcatcag attcaaaatg aagccatagg
     2341 gcagccagac tccatgtatc tgtcgctggt acatgaatac catggggcca gtctgatcag
     2401 aaaatatctt ttgatgggac atgaacgctt ttctgttata ggaaagcagt ttacgtttcc
     2461 gctgacagag acagattccc gtcagggggc tattacggat ggttctgtaa gcagccgggg
     2521 gagaatcatg gctctgtttg aatttgcttc cggaaagacg gcgctttatg atttttcggg
     2581 ggaacagtac cggtctttta tacgcagccg ccatgtgacc gttcgcggac agaaaggaga
     2641 gcttacggat cagattcttt acaggcttac agaagaaaat ttccctgaaa ccctatatat
     2701 tatgccggaa ttagattcac ggtacagaca gcttgaaaca aaacgtttaa gggattgtgt
     2761 ccggggctgg aagcctgagc tgtatctgga tgagattgag gatgaatatg ccattgcctg
     2821 tatgctttat gatatgagag agtatctcga ttcgggaaaa gaaatttatc ctttaaggga
     2881 tgctcttcag gacagctata tggctctgct gctccgggag gctgtcatgt cagagggctg
     2941 tgagattcat tcccagccaa tgccctggaa ttagaaaaac gcttttcgtt taaaaatccg
     3001 gctttcggta cagactatca tcagatctgc agcatgggcg aatgaaaggc ctgtgccgga
     3061 tacttggtaa aaacgaaagg aaagtctatg aaaaaaggaa aaatgtggaa ggcgggggct
     3121 gcagggataa tggctgcggg aacaggagca gtccttatga tggccagccg gaaaaaggga
     3181 cggatgcaca ggcttacgga cagcaattcc tcctgtaaaa ataaagggtc aaagggagaa
     3241 aatcagagga tttattattc caaaggcaat tatgaggcat ttgcccgtcc tgaaaaacct
     3301 gagggaatag aagaaaagtc ggcctggtta atcggcggtg gtctggcttc tctggcggcg
     3361 gcctgttttc tgatccggga tgccggaatg tccggggata aaatccatat tctggaagcc
     3421 tctgaccgta cagggggagc ctgcgacgga attctggatt catcgagagg atatattatg
     3481 cgcggcggac gtgagatgga gaatcatttt gaatgcctgt gggatctgtt tcgatccatc
     3541 ccttcgcttg atacgccggg ggcctcggtg ctggatgaat tttactggct taataaggct
     3601 gatcccaatt attccctctg cagggtaacg gaaaaacagg gaaagcctgc ctgtacacag
     3661 gggtattttg gtctcagcga aaaggcggga cgtcagatta tgaggctgtt ttttacctgc
     3721 gatgaagacc tgtatgataa aaccatagaa gatgtatttg acaatgaagt atttgactcg
     3781 cccttctggc tttactggcg caccatgttc gcttttgaga actggcacag cgctctggaa
     3841 atgaagcttt atctcaggcg ttttgttcat catatcggtg gacttcctga tttttcagca
     3901 ttgaaattta cacgctacaa tcagtatgaa tcactgatcc ttcctctgaa acggtatctg
     3961 gaagaagccg gtgtggaatt tcgctttgga gttgcagtga caaatgtgga gtttgacatc
     4021 agagagggaa aaaagacggc aaaatttctg gactgcatga taaatggcgc aaaagcacag
     4081 attcctctta cagaacagga tctggtattt attacaaatg gaagctgtac agagggaacg
     4141 gtttacggag accaggatca taaaccggac ccgaaaagac agtcggaggc tgaacactgc
     4201 tggacactct ggaaaaatat agcagtacag gatacgtcct ttggacgtcc ggaaaaattc
     4261 tgctccaacc gtgaaaagac ctgttgggaa tcagccacaa ttactacatc ggatgagacg
     4321 atcctttctt atataaaaaa gatagcccga agagatccgc gtacaggaaa ggtagttacg
     4381 ggaggaattg tcagctgtca ggattccggc tggcttttaa gctggacggt caaccgccag
     4441 ggtcagttta aggctcagaa gaaagaagag gtatgcatct ggctctatgg attatttcca
     4501 aatggagtgg gaaatttcgt gcccaagcct atgagtgaat gcacgggaag ggaaatcacg
     4561 gaggaatggc tgtatcatct gggagttccg gctgaccaga ttcatgatct ggctgcttgg
     4621 ggagcggtct gcgttccggc tatgatgccc tatattacgg cctttttcat gcccagaaga
     4681 gagggagacc gcccggatgt aattcctgaa ggaagcgtaa atgccgcctt tatcggccag
     4741 tttgcccaaa ctcccagaga cacggttttt acaacggagt attccgtgcg cacggctatg
     4801 gaggctgttt acggcctgtg cggcgtagac cggggggtac cggaggtatg gggcagcgct
     4861 tatgatatga gagagctttt aaatgcctct gtgaagctta tggatggagt ttcaccttta
     4921 aagaaaaggc ttccggcatg ctttgagaaa gcagggaaaa tggcgattaa aaaaattgaa
     4981 catacggaga taggagaact tctcagggaa tgcggagtgc tgtagcatta taaacaacag
     5041 gcaaaatcct gtataaaagg gttctgcctg aatgtttgtc tcagcggtat gggacagagg
     5101 ccgcttactg taatttggtt cctgtaagca tttcatagct ctgaagatat ttgtcaatag
     5161 ttttttccac aatctcctca ggaagggtcc agtcattgcc cggatgagcg gtcagccagt
     5221 ctctggcaaa ctgcttgtca aaggaaggct gggagtggcc gggttcatag ccctgtgcag
     5281 gccagaagcg ggaactgtca ggcgtcagca tctcgtcgcc taaaaccaca ttgccatcct
     5341 catccagacc aaattcaaat ttggtatcag caatgatgat gccacggctt aatgcatagt
     5401 ctgcacattt tttatagaga gcgatcgtat aatcgcggat cagggtcgcg taatgctcgc
     5461 cctttccagg aaaatatttc tccaggtggg aaacactctg ctcaaaagaa atattttcat
     5521 catgatcacc gatttcggcc ttggtagaag gggtgtagat gggttcagga agcttttctg
     5581 attcctgtaa gccttcaaga agatggatgc cgcatacggt gccgttttcc tgatagctct
     5641 tccagccgct gcctgtgata tagcctctca cgatgcactc taccgggatc atggtaagct
     5701 ttttgcacat catgcttttt ccttcaaact caggctgctg gaaaaattca ggcatatctt
     5761 ttacatctac ggaaatcatg tggttgggaa ggatgtcctt tgtaaaatca aaccagaatc
     5821 tggacatctg ggtcagtacc gtacccttcc cggccacaac gttgtgcagg attacgtcaa
     5881 agcagctgat ccggtcagta gcaaccataa tcaggctgtc gccgttgtca taaatctcac
     5941 gaacttttcc ttctttgaac ggtttcatgt catatacctc gctatatgta aaattttgat
     6001 gtagcttcta tctaaggata aacgaaatcg gctgtaaaaa tcaataccca gattgaaaat
     6061 tataccgcag cagacgcaaa aaaacaaagg ttctctttta tgcacaataa atagccgtaa
     6121 aaataaaata gtcaggacaa atttaccaca ggctaaaaag aatgattgat attatggcaa
     6181 atccgcgtta aactaacgga gaagtgcaaa aaagacagga gtttgacctt atgatacgac
     6241 agaaaaaaac acattccaaa aagaaaggtg ctataaggcc gggccttgtg attacttccc
     6301 ttcttcttgc cgtcagtatt ggagtaacgg gaacggcggt ttattttaaa gatatagtaa
     6361 atctgtatct ggcagcagac cgtaccagag tatcggaaga ggagatggaa aagacagcgc
     6421 aggagagtca gcttttgtct cacaggatac aggaagaggg aagcgtactc ttaaaaaaca
     6481 ccaaagatac acttcctctt tctgattcgg tccgccaggt aaatgtattt ggctgggcgt
     6541 ctacccagtg gctgggcgga ggctccggtt ctggacggat cacccaggtt aagacggatt
     6601 ttctggctgc cttaagggat gcgggggttt cttataatga agaactgata gggatgtacc
     6661 gtgaatttca gcccaaacgt ccttatagtg ataccttaaa cagctggccg caggaatcct
     6721 gccgccttta tgaacctcat atttcagacc gggagtatta tacagaggaa cttttagacc
     6781 atgccagaga ctattcggac acagcgatcg ctgtgatcgg ccggtttgca ggcgagagta
     6841 atgactgtcc caaggtacag tataagcaga caaaaaaagg gggagaaatt acagaggatc
     6901 caacccggac ctatctggat ctgtctaaag aggaagagga tcttttgcgc tatctgggtg
     6961 taagctataa aaatgtgatc cttgttttaa atacgggaaa tgtgatggct ctgggtgcga
     7021 tcgaacgtct ttccggcatt gatgcctgcc tttatgcagg ccttacggga gaatcaggag
     7081 cgggcgtact gccgaaaatc ctctgggggc gcgtaagccc ttccggacgg acaacggata
     7141 cctgggctta tgatttttcc acagccgcca gctacgtcaa tgcaggagga gagggtgtgg
     7201 gacgctatac caacgcagag acgctctatc ctgcagatgg cacgaaatgc ggcaatctgg
     7261 gagaaaattt tgcctatga
//
LOCUS       DBMDUH010000135         7261 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_4415_length_7261_cov_8.428073, whole genome shotgun sequence.
ACCESSION   DBMDUH010000135 DBMDUH010000000
VERSION     DBMDUH010000135.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 7261)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7261)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7261
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4415_length_7261_cov_8.428073"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /gene="tadA"
                     /locus_tag="ACZPCG_10535"
     CDS             complement(<1..563)
                     /gene="tadA"
                     /locus_tag="ACZPCG_10535"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HIR1285529.1"
                     /translation="MTEQEIALANAKGLETRRRRWEQRNEKKRLAALAAEEAARAARR
                     ADEAFMKEAVRQAKKAAAIGDVPIGCVIVKEGRIISRGYNRRNADKTVLSHAEITAIK
                     RACKKEGDWRLEDCTLYVTLEPCPMCAGAIVQARIPRVVIGSMNAKAGCAGSVLNILK
                     EPGFNHQVKVETGILGEECSCLMTDFF"
     gene            664..1359
                     /locus_tag="ACZPCG_10540"
     CDS             664..1359
                     /locus_tag="ACZPCG_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005952066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HIR1285530.1"
                     /translation="MKLCGLQKTTLLDFPGKVAATIFTGGCSFACPFCHNSELLGRDT
                     EALCSQEEVMTFLEKRKNILDGVCITGGEPTLQPDLEDFIRFVRSLGLAVKLDTNGYQ
                     PNVLINLCEKGLLDYVAMDIKAGKSHYAHVSGRTGLNLERIDQSIRFLLNGSVPFEFR
                     TTVVKGLHDKSDFEEIGPWIQGCGQYFLQSYQESGQVLSPDSCKSFSREEMEEFARIV
                     SPYAGIVRLRGID"
     gene            complement(1375..1821)
                     /locus_tag="ACZPCG_10545"
                     /pseudo
     CDS             complement(1375..1821)
                     /locus_tag="ACZPCG_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577112.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6128 domain-containing protein"
     gene            2869..3594
                     /gene="nagB"
                     /locus_tag="ACZPCG_10550"
     CDS             2869..3594
                     /gene="nagB"
                     /locus_tag="ACZPCG_10550"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HIR1285531.1"
                     /translation="MRICKAKNYDEMSRQAANVISAQVIMKPNCVLGLATGSSPIGTY
                     KQLIEWYNKGDLDFSNVKTVNLDEYKGLTKDNDQSYYYFMYNNLFKHINIDLNNTNIP
                     DGTEPDSAIECARYNRVIDDIGGIDLQLLGIGHNGHIGFNEPDSEFAKMTHCVDLTES
                     TIEANKRFFASIDDVPRQAYTMGIKTIMQAKTILLIASGADKARILREALCGPVTPQV
                     PASILQLHNNVIVVADEAAMAEF"
     gene            complement(3686..4237)
                     /locus_tag="ACZPCG_10555"
     CDS             complement(3686..4237)
                     /locus_tag="ACZPCG_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptM family lipoprotein"
                     /protein_id="HIR1285532.1"
                     /translation="MKKWLLGMMAAVMAVSLAACTPTTEKQKEETTTVSAENPEPAVV
                     PTTGGASDKVPDPNVEPVAVISIYHKGDGDSLVQEMDSLDDDDLNAQALVDRMIGYGV
                     FTEGTEVISFDKTGEEENAKGVLNLNQAKNNEGVTETQFLVEIGNTFIENFELSTLTV
                     QINGQDMEGASALEYVTAFEDVE"
     gene            complement(4254..4712)
                     /locus_tag="ACZPCG_10560"
     CDS             complement(4254..4712)
                     /locus_tag="ACZPCG_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002605672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR1285533.1"
                     /translation="MKGRDVETYDTLNDVLVNLFRDINDIEEKAITSYENKDLTSNDM
                     HVIHAIGVGTKKNMSSIAKELSVTVGTLTISMNSLVKKGYVVRSRSTEDRRVVHISLS
                     EKGIKAYEHHKYFHEQMIQGVMDVLTEEEMNALVKALTKLTSWFRSFEKN"
     gene            complement(4799..6235)
                     /gene="murD"
                     /locus_tag="ACZPCG_10565"
     CDS             complement(4799..6235)
                     /gene="murD"
                     /locus_tag="ACZPCG_10565"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004465252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HIR1285534.1"
                     /translation="MIEKIDPWVRGKKILLLGFGREGKSTWEVLRRLGTFQSVDIADQ
                     SAPGELPEGIGKWISGPDYQSCLNDYDVVFKSPGVVLEKPVQDYSCQILSQTEVFFRC
                     FRDQIIGITGTKGKSTITTLLYHLLKHAGYDTVLAGNIGIPVFDLMEEIVPETKIVCE
                     LSCHQLEYMTVSPHIAILSNIHEEHLDHYGTMEKYVRAKEHIFLNQTPEDVLICSVQC
                     LPEPGECVSRLITAKSSFPEPPLERFWKTEGNLADRKKREEAPVLYVEEKDGVTSICY
                     EGRRYKIPTGQIRLLGQHNYFDIGMAYAVCSLLGMAEETFTEGLKEYEPLPHRLQFLG
                     EIQGVKYYDDSISTICETAIQALKTLKDTDTILIGGMDRGIDYRELIEYLLQSQVPHI
                     ILMEATGKRIFEEIAADYPDFENPSRLLLAEHLKDAVCLAKKVTRPGRSCVMSPAAAS
                     YGIFRNFEERGEEFKRLVLENEQQDTVF"
     gene            complement(6254..>7261)
                     /locus_tag="ACZPCG_10570"
     CDS             complement(6254..>7261)
                     /locus_tag="ACZPCG_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6395 domain-containing protein"
                     /protein_id="HIR1285535.1"
                     /translation="SDVVCDDPEFIFKQGRLSSVSGSEKGVLVPVGGGKDSAVTLELL
                     KGAGVPVYAYIINSRGATIHTTEAAGLDEDHVIYVKRTLDQNMLELNRQGYLNGHTPF
                     SALVAFSGIMAARMAGLSYVALSNESSANESTVKGSTVNHQYSKSFKFEEDFHRYEAE
                     YLPGSAYYFSMLRPLSEFQIARFFAQQKQYHAIFRSCNAGSKTDSWCGHCPKCLFVYL
                     ILSPFLSEEEVEKIFGRNMLQDEEMKPLLDQLTGIQEEKPFECVGSRDEINSAIVLTI
                     ERMEKEGKKLPLLFEHYKETGLYERYQTEKNKFSAYFDSSNLVPEPWEKFVRERCTKE
                     A"
BASE COUNT         1803 a         1948 c         1455 g         2055 t
ORIGIN      
        1 ttaaaaaaat ccgtcatcag acaggaacat tcttccccaa gaatacctgt ttctactttt
       61 acctgatgat taaatcccgg ctctttaaga atatttaata ccgaaccggc acagcccgcc
      121 ttcgcattca tggatccaat gacaacacgg ggaatcctgg cctgtacaat ggctccggcg
      181 cacatgggac agggctccag cgtgacatac agcgtacaat cctccagccg ccagtctccc
      241 tcttttttgc aggctctttt tatggctgta atttctgcat gggaaagaac cgttttatct
      301 gcattccgac ggttgtagcc tctggaaatg atacggccct cttttacaat cacgcagccg
      361 attgggacgt ctccaatcgc agccgctttt tttgcctgac gaaccgcttc cttcataaag
      421 gcttcatctg cccggcgggc tgctcttgca gcctcctctg ccgcaagggc agccagacgt
      481 tttttctcat tcctctgctc ccagcggcgt ctgcgggttt caagtccctt tgcatttgcc
      541 agagcaattt cctgttctgt catacgatca atctccttat atgcgttgag gtcaaacgac
      601 ttgtgaccct aatatcctat tataaataaa atcataaatt ttgcaaagga aggatcaatc
      661 aatatgaaat tatgcgggct tcagaaaaca acgcttctgg actttccggg aaaggtagct
      721 gccacgatct ttaccggcgg atgcagcttt gcctgccctt tctgccacaa cagcgagctg
      781 cttggacgcg atactgaggc actttgctct caggaagaag taatgacatt tttagagaaa
      841 cgaaagaaca tattggatgg tgtctgcatc accggaggag aaccaactct gcagccggat
      901 ctggaggatt ttatacggtt tgtccgcagt cttgggctgg cagtaaagct ggatacaaac
      961 ggataccagc caaatgtgct cattaatctt tgcgaaaaag ggcttttaga ctatgtagct
     1021 atggatatca aagcaggaaa aagccattat gcgcatgtaa gcgggcgcac cgggctgaat
     1081 ctggaacgaa tcgaccaaag tatccgtttt cttttaaatg gttctgtccc ctttgaattt
     1141 cgtacgaccg ttgtaaaagg cctccatgat aaaagtgatt ttgaggaaat cgggccatgg
     1201 atccaggggt gcggacaata ctttctgcag tcttatcagg agtccggcca ggtactgtcc
     1261 cccgattcct gcaaaagctt ttcccgggaa gaaatggagg aatttgcccg gattgtatcc
     1321 ccctatgccg gaatcgtcag gctccgtgga atcgactgat ccatgcatgg cctgtcagtc
     1381 cgtattttcc atactgacat ctgcatacca gtaaccgaac cggccgtttt gaaccggctg
     1441 ttttttggca agaacaaaat gcggaaaccc gaacatggaa gccatatatt tttcgctgct
     1501 gtaataatgg cccggaaccc ccagaatata gcgtacccgt ttgcgcgcct catcgcctac
     1561 acgggcaaat accagatacc ggtaattata atagccatga agcagaaagc tgttgtttcc
     1621 atacgtccag ttttccctcg gtaaaaggcc gatatcctga ggacggatgg tgagaatctc
     1681 gatggtaccc ggactgtcaa aagcctgtat cttgggatag cgctttcgga aggcttccca
     1741 caaaagctcc ggactgccgg cgctttcttc ctgctccaga cgcgccagct tttcgggatt
     1801 ttctaaagga aattcctcct cctgaggcaa agaaacgcga gccggatggg agactaatgg
     1861 ctctgatggc tcctgcgctg cctcctctct ggcctgttct ttttcttcca caggcttctc
     1921 atgtccggcc ggcagtattc ccaatatatt tcctacttta tttaggttct gcaaaagcat
     1981 ttgggtaccg cttcccattc tctgattatc ttccggatga tcctgaccgc ctgcttccac
     2041 atcttcttcg ctatgcgggg cctctgatcc ttccttttca tcttttaaag ccatctctgc
     2101 cttttcaatc tgtatttcat cttcctccgg ctctttcgtc tcctccctgg ccgcttcccg
     2161 ttccttttct tctaaaacta atttcgctct ctccgcctcc agacgttcct cctctgcctg
     2221 aatttcctga ttcagttccg tcagctttct atggatctgc tcgctctcat cccctacatt
     2281 ctgagacgtt acctctgcca gatctacctc tgcggcctga gtaactgcat cctcccaaat
     2341 cgtcttatac acccgccagt cttcttcctt ttcatggacg gaaaggccat aacagatttc
     2401 catagcacag ccagatcctc caatattttc catcggaatc acagctctga actcgcccgc
     2461 tccattgtga ataaaaatat tacccagaaa aatctcttta tcaggcgcca gcagatacac
     2521 ccccagatcg cttcctcctg aataaacctt ttttacattt acattcagcc tacactggcc
     2581 gcttctggtt tccagcttgg caaatcctat atttttcccc tttgcgcttc cttcatatgc
     2641 ataaatataa gaaatcaatc ttctataatt caccattttc ctactccttg caccatttcc
     2701 aaatcgctcc accccagacc tgtctgcctg tccccagatg gcagcccagt ttccgctgtt
     2761 accattattt atatgttttt ccccttgaaa gtatgttgag aaaatgctat tctatatgga
     2821 gaaaattgca aaaataattt tcattttacg gaaaggttgg atattattat gagaatctgc
     2881 aaagcaaaaa attatgatga aatgagccgc caagctgcca atgtcatttc cgcacaggta
     2941 atcatgaagc ctaattgcgt gctgggactg gctaccggtt cttcaccgat cggtacctac
     3001 aagcagctga tcgaatggta caacaagggc gatctggatt tctccaatgt aaagacagta
     3061 aacctggacg agtacaaggg acttacaaag gataacgatc agagttacta ttattttatg
     3121 tataataatc tctttaagca tatcaatatt gacttaaata acacaaatat tcctgatgga
     3181 acagagccgg acagcgccat tgagtgtgcc cgctacaacc gtgtgatcga tgatatcggc
     3241 ggcattgatc tgcagctttt aggaatcgga cacaacggcc acatcggttt caatgagcct
     3301 gattctgaat ttgcaaaaat gactcactgc gttgatttaa cagagagcac cattgaggca
     3361 aacaagcgct tcttcgcttc tatcgatgat gttcccagac aggcttatac catgggtatc
     3421 aagaccatca tgcaggcaaa gacaatcctt ctgattgcca gcggtgcaga taaggcccgc
     3481 atcctccgcg aagctctctg tggccctgta acccctcagg ttcccgcttc tatcttacag
     3541 cttcacaata atgtaatcgt agtggctgac gaagcggcta tggctgagtt ttaatcatta
     3601 aaaagcacta aaaaactgac agaaaacggt tcatctaaag ccgcttcctg tcagttttat
     3661 tttatctgtt ttatttgaag cttatttact ccacatcctc aaacgccgtc acatattcaa
     3721 gcgcggacgc gccttccata tcctgtccat tgatctggac tgtaagtgtg gaaagctcaa
     3781 aattctctat aaatgtattt ccaatttcta ccaaaaattg agtttctgta acaccttcgt
     3841 tatttttcgc ctgatttaag ttcagaacgc cttttgcatt ttcctcttcg ccggttttat
     3901 caaaggaaat aacctctgtt ccctcggtga aaacgccgta tccgatcatc ctgtctacca
     3961 gtgcctgagc attgaggtcg tcgtcgtcaa gagaatccat ctcctgaacc aggctgtctc
     4021 cgtcgccttt gtggtaaata gaaataacag ctacaggttc tacatttgga tcgggaacct
     4081 tgtctgaagc cccgccagta gtcggaacga ctgccggttc cggattttct gcagatactg
     4141 tggttgtctc ctccttctgc ttctcagtag taggcgtgca ggctgccaga gaaactgcca
     4201 taactgccgc catcataccc aataaccatt ttttcattta aaaactgcct cctttaattc
     4261 ttctcaaagc tacggaacca ggaggtcagc tttgtcagcg cttttacaag agcgttcatc
     4321 tcttcctctg tgagaacgtc catcactccc tgtatcatct gttcatgaaa atacttatgg
     4381 tgctcgtatg ccttgattcc tttttcagaa agtgaaatat gaaccacccg cctgtcctct
     4441 gtgctgcggc tccgcaccac atagcccttt tttacaaggc tgttcataga gatggtcagt
     4501 gttcctaccg tcaccgacag ctcctttgcg atggatgaca tatttttctt cgttccaacg
     4561 ccgatcgcat ggatgacatg catatcatta ctggtcagat ccttgttctc atagctggta
     4621 atggccttct cttcgatatc attgatatcc cggaacagat ttaccaatac atcgttcagt
     4681 gtatcgtaag tctccacgtc tcttcctttc actttcctca gtatccttct cagatacgct
     4741 ctactgattg tacagtattt tcctgtgtca tacaagtcct aaaatgctta taaaattctt
     4801 aaaaaactgt gtcctgctgc tcattttcaa gcaccagacg tttaaattcc tcgccgcgct
     4861 cttcaaaatt ccggaatatg ccgtagctgg cagctgcagg cgacataaca cagctgcggc
     4921 caggccttgt aaccttcttg gcaagacaaa ctgcgtcctt cagatgctct gccaacagca
     4981 gtctggacgg attttcaaaa tcaggataat ctgccgcgat ttcctcaaaa atcctctttc
     5041 ctgtggcctc catcaatata atatggggca cctgcgactg taacagatat tcgatcagct
     5101 ctcggtaatc aattcctctg tccatgccgc cgattaagat ggtatccgtg tccttcagag
     5161 tttttaacgc ctgaattgct gtctcacaga ttgtagaaat ggaatcatca taatatttaa
     5221 cgccctgtat ttctcccaga aattgtaagc gatgaggaag gggctcatac tccttaaggc
     5281 cttctgtaaa cgtttcctct gccattccca gcaggctgca cactgcataa gccataccaa
     5341 tatcaaaata attatgctgg cccaaaaggc ggatctgacc ggtcgggatc ttataacgac
     5401 ggccctcata gcagatggat gtaactccat ccttctcctc cacatacaaa acaggggctt
     5461 cttcccgttt ttttctgtct gccagattcc cctctgtttt ccagaaacgc tcaagcggcg
     5521 gctcagggaa ggaggacttt gcagtaatca gtctggaaac acactccccc ggctcaggca
     5581 gacactgaac gctgcagatc agcacatcct ccggggtctg atttaagaaa atgtgctcct
     5641 tggcccgtac atatttttcc atcgtaccat agtgatccag atgctcctca tggatatttg
     5701 ataaaatggc aatatggggt gaaacggtca tgtattccag ctgatggcag gaaagctcac
     5761 acacaatctt tgtttccggt acgatctctt ccataagatc gaacacggga attccgatat
     5821 ttcctgccag aaccgtatcg tatcctgcat gtttgagcaa atgatacagc agcgtggtaa
     5881 tcgtactctt tcccttggta cccgtgatac cgataatctg atcgcggaag cacctgaaaa
     5941 acacctctgt ctgggaaagg atctgacagc tgtaatcctg caccggcttt tccagtacca
     6001 ctccgggact tttgaaaacc acatcataat cattcagaca gctctggtaa tccggcccgc
     6061 tgatccattt tccaatccct tccggcagct ccccgggcgc cgactgatcc gcgatatcca
     6121 cggactgaaa ggttcccaga cgccgcagca cctcccatgt ggatttcccc tctcttccaa
     6181 agcccagcaa aagtatcttt ttcccccgta cccagggatc aattttctct atcacagtcc
     6241 gtctcctcgt ctcctatgct tcctttgtac acctttccct gacaaatttt tcccagggtt
     6301 caggcaccag attgctgctg tcaaaataag cggaaaactt atttttctct gtctgatacc
     6361 gctcatacag gcctgtttct ttataatgtt caaataaaag cggaagcttc ttcccctctt
     6421 tttccatccg ctcaatcgtt aatacaatgg cagaattgat ctcatcccgg cttcccacac
     6481 actcaaaggg cttttcctcc tgaatacccg taagctgatc caaaagaggc ttcatctcct
     6541 catcctgcag catatttctt ccgaaaatct tctccacctc ttcctccgaa aggaacgggg
     6601 acaggatcag atatacaaag aggcacttgg ggcagtgtcc gcaccagctg tctgttttgc
     6661 tgcctgcatt gcagctcctg aaaatggcat ggtactgctt ctgctgtgca aaaaagcggg
     6721 caatctgaaa ctcgcttaag gggcgcagca tactgaaata gtacgcgctt cctggaagat
     6781 attctgcctc gtagcggtga aaatcctcct cgaatttaaa actcttggaa tactgatgat
     6841 tgaccgtact tccctttacg gtgctctcat ttgcactgga ttcatttgac agtgccacat
     6901 aggaaagtcc ggccattctg gctgccataa tgcccgaaaa agctaccagc gcagaaaacg
     6961 gagtatggcc gtttaaatag ccctgacggt ttaattccag catattctga tccagcgtac
     7021 gttttacata aatcacatga tcctcatcca gtccggctgc ctctgtggta tggattgtgg
     7081 ctcctctgga attgataata taggcatata caggaacgcc tgcgcccttt aaaagttcca
     7141 gggtcactgc ggaatcctta cctcctccga caggaaccag cactcccttt tccgaaccgc
     7201 tgacggagga aagacgcccc tgtttaaaaa taaattccgg atcatcacag acaacatcgg
     7261 a
//
LOCUS       DBMDUH010000136         7257 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_4418_length_7257_cov_5.088038, whole genome shotgun sequence.
ACCESSION   DBMDUH010000136 DBMDUH010000000
VERSION     DBMDUH010000136.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 7257)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7257)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7257
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4418_length_7257_cov_5.088038"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..893
                     /locus_tag="ACZPCG_10575"
     CDS             <1..893
                     /locus_tag="ACZPCG_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HIR1285536.1"
                     /translation="MCGSAGTGKSMVASRIPTILPGLTREEDIEISRVYSVCGKLPPG
                     RPLLSKRPFRSPHHTISPQGLTGGGRFPVPGEFSLASGGVLFLDELPHFSRGAIEALR
                     QPMEERKIVISRVWGSCEFPADFLIVGAMNPCPCGFFPNRNRCSCTEAQIQGYLRRLS
                     RPILERFDMGVEAAPVSFRELEETPAENESSKEIRTRVERAVKIQKKRFEGTKIRFNS
                     RMGPTEIRRFCPLGEREREFAEKVYEIRGLSARSYHKVLKTARTIADLAGEKNIRREH
                     LAEAFGYRTLEGRLWGREKN"
     gene            872..1984
                     /gene="dprA"
                     /locus_tag="ACZPCG_10580"
     CDS             872..1984
                     /gene="dprA"
                     /locus_tag="ACZPCG_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007718022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HIR1285537.1"
                     /translation="MGTGEELERLFLHWLIRMEGMGAVTVKRIGAFAGSYRNAYYIEG
                     MELQKAGILKTEESCIRYDAWKKEFYPMEEEYYRLAEKGIRFITHLDPDYPSRLLSMY
                     DFPAALYVKGDLPKEDEPAAAIIGARECSAYGRQTAEHMAKELAEAGVTIISGLARGI
                     DGAGHKGALDGGGKTFAVLGCGVNICYPRSNYSLYTQIGEQGGLISEYPPDTPPLARN
                     FPMRNRIISGLSDAVLVIEARKKSGSLITAQTGLDQGKDIYAVPGRITDSLSEGCNQL
                     IASGAFVLLSAENLLENMGISYKKKDRAGGKIEKGLAKREKMVYSCLDSEPLHAEEIA
                     ARAGLSVSQSMDVLLTLELEGYAVRTTGLYYVRGDL"
     gene            2046..4157
                     /gene="topA"
                     /locus_tag="ACZPCG_10585"
     CDS             2046..4157
                     /gene="topA"
                     /locus_tag="ACZPCG_10585"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004611389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HIR1285538.1"
                     /translation="MANHLVIVESPAKVKTIKKFLGSSYEVDASGGHVRDLPKSQLGF
                     DPEHDYEPKYITIRGKGDVLAKLRKEVKKADKIYLATDPDREGEAISWHLMKALKLDE
                     VKNKSVYRISFNEITKNAVKASLKAPRTLDMNLVNAQQTRRMLDRMVGYRISPLLWAK
                     VKRGLSAGRVQSVALRMICDREDEINAFIPEEYWNLDASFKIAGMKKPLTARYYGPAD
                     SGKEEKAKGAKTVIRSKAELDAVMKDLENVSYLVDEVKKGERVKKAPIPFTTSTMQQE
                     ASKALNFSTQKTMRLAQQLYEGVEVKGHGTIGLITYLRTDSTRIAEEADAAARAFVKE
                     HYGENYMAAGEPAKKNAGKIQDAHEAIRPTDISLTPVMVKESLPRDLFRLYQLIWKRF
                     AASRMAPAVYETTMVKIRGGNQLFSAAASRLSFDGFMSVYVDADDEEEKGQTMGAMEK
                     GSALSLEKLEPSQHFTQPPAHYTEASLVKALEEQGIGRPSTYAPTITTIIARRYVAKE
                     NKNLYVTELGEVVNRIMKQSFPSIVDVSFTANMESLLDKVADGTVAWKTVVSNFYPDL
                     DEAVTIAEKELESVKIEDEVTDEICELCGRNMVIKYGPHGKFLACPGFPDCKNTKPYL
                     EKIGVPCPKCGKEVVRKKTKKGRLYYGCEANPDCDFMSWQKPSTQKCPKCGSYMVEKG
                     SKLLCADETCGYRMDPAEKEK"
     gene            4277..5065
                     /gene="codY"
                     /locus_tag="ACZPCG_10590"
     CDS             4277..5065
                     /gene="codY"
                     /locus_tag="ACZPCG_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HIR1285539.1"
                     /translation="MSVQLLDKTRKINKLLHNNNSHKVVFNDICKVLSEILLSNILVI
                     SRKGKVLGVSLCPGVDEIEELIEDQVGGFVDKMLNERLLSVLSTKENVNLATLGFAEE
                     NVKKYQAIITPIDIAGERLGTLFIYKSDSQYDIDDIILSEYGTTVVGLEMMRSVNEEN
                     AEETRKVQIVKSAISTLSFSELEAIIHIFEELNGNEGILVASKIADRVGITRSVIVNA
                     LRKFESAGVIESRSSGMKGTYIKVLNDVVFDELKAIKASNSNLK"
     gene            5282..6034
                     /gene="rpsB"
                     /locus_tag="ACZPCG_10595"
     CDS             5282..6034
                     /gene="rpsB"
                     /locus_tag="ACZPCG_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HIR1285540.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAPYIYTERNGIYIIDLQ
                     KSVGKVDEAYKAVSDIAAEGGTILFVGTKKQAQEAIKTEAERCGMYYVNERWLGGMLT
                     NFKTIQSRIARLKEIEAMSEDGTFDVLPKKEVIELKKEWDKLEKNLGGIKDMKKLPDA
                     IFVVDPKKEHICIQEAHTLGITLLGIVDTNCDPEELDYVIPGNDDAIRAVKLIVSKMA
                     DAVIEAKQGEVLEGAMEIEVPADAFEAEGAEA"
     gene            6065..7003
                     /gene="tsf"
                     /locus_tag="ACZPCG_10600"
     CDS             6065..7003
                     /gene="tsf"
                     /locus_tag="ACZPCG_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HIR1285541.1"
                     /translation="MAVTAAMVKELREMTGAGMMDCKKALAATDGDMEKAVEFLREKG
                     LAGAAKKAGRIAAEGIVDTLLTADEKTAVVVEVNAETDFVAKNAVFQAYVADVAAQAL
                     ASSAADMDSFMAEKWAKDESLTVKEALSSQISIIGENMNIRRFEKVTEENGFVASYIH
                     AGGKIGVLVDVETNVVNDEIKEMARNVAMQAAALKPQFTSRDEVDEAFIEHEKSILMA
                     QIQNDPKEASKPEKVIQGMIQGRINKELKEFCLLDQVYVKAEDGKQSVAQYVAAVAKA
                     NNADVKIKKFVRFETGEGLEKKNEDFAAEVAAQMGK"
BASE COUNT         2094 a         1459 c         2023 g         1681 t
ORIGIN      
        1 tgatgtgcgg cagtgcggga accggaaaat ctatggtagc ctcccgtatc cccacgattc
       61 ttcctggtct caccagagaa gaggatatag agatatccag agtttacagc gtctgcggca
      121 agcttccccc tgggcgtccg ctgctttcta agcggccctt ccgcagtccc caccacacga
      181 tttctcctca gggccttaca ggaggcggcc gctttccggt tcccggagaa ttttcacttg
      241 cttcgggagg cgtgctgttt ttagatgagc tgcctcattt cagccgcggc gccatagagg
      301 cgctgcgtca gcctatggaa gaacggaaaa ttgtgatcag ccgtgtctgg ggaagctgtg
      361 agtttccggc ggattttctc attgtgggag ccatgaatcc ctgcccctgc ggcttttttc
      421 cgaaccgtaa ccggtgcagc tgtacagagg cgcagatcca gggatattta agaaggctgt
      481 cccgccctat tttagagcgc ttcgatatgg gggtggaggc ggctcccgtt tcctttcggg
      541 agctggaaga gactccggcg gaaaatgaat cctcaaaaga gatccgcaca cgggtggaac
      601 gggccgtaaa aatacagaaa aagcgctttg aaggaacgaa aatccgcttt aacagccgga
      661 tggggccgac ggaaatccgg cgtttttgtc cgttgggaga aagggaacgg gagtttgccg
      721 aaaaagttta tgaaataaga ggattaagtg ccagaagtta tcataaggtt ttaaagacag
      781 ccagaaccat agcggatctg gcaggagaga aaaatatcag gcgggaacac ctggcagagg
      841 catttggata ccgcacactg gagggacgat tatggggacg ggagaagaat tagaacggct
      901 gtttttgcac tggctcatcc ggatggaggg aatgggagct gtgactgtaa aacggatcgg
      961 agcatttgca ggaagctatc gaaatgcata ttatatagaa ggaatggaac ttcaaaaagc
     1021 ggggatctta aagacagagg aaagctgcat acggtatgat gcctggaaaa aggaatttta
     1081 tccgatggaa gaggaatatt accgtctggc agaaaaagga atccgtttta tcacgcatct
     1141 ggatccggat tatccgtccc gtcttctttc catgtatgac tttccggctg ctttgtatgt
     1201 gaaaggagat ctcccaaaag aagatgaacc ggcggcggct attatcggtg ccagggagtg
     1261 ttcggcttat ggccggcaga cggcagagca tatggcgaaa gagctggcag aggcaggcgt
     1321 aacgatcatc agcggcctgg ctcggggaat cgatggagcc ggacataaag gcgctttgga
     1381 tggaggtgga aaaacctttg cagttctggg ctgcggcgta aatatctgtt atcccagatc
     1441 caattacagc ctgtatactc aaattgggga acagggcggt cttatatcgg agtatccccc
     1501 ggataccccg cctctggccc gcaatttccc catgagaaac cggatcatca gcggtctttc
     1561 agacgcagtt ttggtgatag aggccagaaa aaagagcggt tccctgatta cagcacaaac
     1621 aggactggat cagggaaagg atatttatgc ggttccggga cggattacag attccttaag
     1681 cgaaggctgt aatcagctta tcgcttccgg tgcttttgtc ctcctgtctg cggaaaacct
     1741 tttggaaaac atgggcattt cctacaaaaa gaaagaccgg gcagggggaa aaattgagaa
     1801 gggacttgca aagcgtgaaa aaatggtgta tagttgcttg gattccgaac cgctccatgc
     1861 agaggaaatc gcagcgaggg caggactttc tgtaagccag tccatggatg tccttctcac
     1921 tctggagctg gaaggctatg cagtgcggac aacaggtctg tactatgtca gaggggattt
     1981 atgatatggc cccaagggga aactgacaga aagaatcagt aacacaaaat aaggagtttt
     2041 acaccatggc gaatcatctg gtaatcgtag aatcacctgc aaaggtgaaa accataaaaa
     2101 agtttctggg aagcagctac gaggtagacg cttccggagg ccatgtaagg gatctgccta
     2161 aaagccagct tggctttgat ccggagcatg attatgaacc gaaatatatt acgatccgcg
     2221 gaaagggaga tgttcttgcg aagctgcgca aggaggtaaa aaaggcagat aagatctatc
     2281 tggcaacgga cccggaccgt gagggagaag ccatttcatg gcatctgatg aaggctttaa
     2341 agctggatga ggtaaagaat aaatccgttt accgcatcag ctttaatgag attactaaaa
     2401 atgcagtaaa ggcttcctta aaggctccga gaacgctgga tatgaatcta gtcaatgctc
     2461 agcagacccg caggatgttg gaccgtatgg taggctaccg catcagtccg cttttatggg
     2521 caaaggttaa gagaggctta agcgccggcc gcgttcagtc tgtggccctg cgcatgatct
     2581 gcgaccgtga ggatgagatt aatgcattta ttcctgagga atactggaac ttggacgcgt
     2641 cctttaaaat tgccggaatg aaaaagcctc tgactgcccg ttattacggc cctgcagaca
     2701 gcggaaagga ggaaaaggca aagggcgcaa agacggtgat ccgcagcaag gcagagctgg
     2761 atgcggttat gaaggattta gaaaacgtat cctaccttgt ggatgaggtg aaaaagggag
     2821 agcgtgtgaa aaaagcaccg attccgttta ccaccagtac catgcagcag gaagcctcaa
     2881 aggctcttaa tttttcgact cagaagacca tgcgcctggc tcagcagctt tatgagggcg
     2941 tagaggtaaa ggggcacggc accattggtt tgatcactta tctgcgaacc gattctaccc
     3001 gtatcgcgga agaagcagat gctgcggcaa gagcctttgt aaaagagcac tatggagaaa
     3061 actatatggc tgcaggcgaa ccggccaaaa agaatgcggg aaagatccag gatgctcatg
     3121 aggctatccg ccctaccgat atatccctta ctcctgtaat ggtaaaggag tctcttccaa
     3181 gagacctttt ccgtctgtat cagctgatct ggaagcgttt tgctgccagc cgcatggctc
     3241 cggccgtgta tgagacgacc atggtaaaaa tccggggagg aaaccagctg ttcagtgcag
     3301 ctgcgtcccg gctgtccttt gacggtttta tgtctgtata tgtagatgca gatgatgaag
     3361 aggagaaggg ccagaccatg ggagccatgg aaaagggaag tgcgctttct ctggaaaaac
     3421 tggagcccag ccagcatttt acccagccgc cggctcacta tacagaggct tcccttgtaa
     3481 aggcgctgga ggagcaggga atcggacgtc ccagtaccta tgcgcccacc attactacca
     3541 ttattgccag acgctatgtt gcaaaggaaa ataaaaatct ttatgtgaca gagctgggag
     3601 aggtagtaaa ccggatcatg aagcagtcct tcccaagcat tgtagatgtg agctttaccg
     3661 caaatatgga atcccttctg gataaggtgg cagacggcac ggtggcatgg aaaaccgtgg
     3721 taagtaattt ttatccagat ctggacgagg cggtaacgat cgcggaaaag gagctggaat
     3781 cggttaagat cgaggatgag gttacggatg aaatctgtga actctgcgga aggaacatgg
     3841 tgatcaaata cggtccccat ggaaagttcc tggcctgccc tggctttccg gactgcaaaa
     3901 atacgaaacc ctatctggag aagatcggcg ttccctgtcc caagtgcggc aaagaggtgg
     3961 tgcgcaagaa gacgaaaaag ggccgtcttt attacggctg tgaggcaaat ccggactgtg
     4021 acttcatgtc ctggcagaag ccttccactc aaaaatgtcc caagtgcggc tcttatatgg
     4081 tagaaaaggg cagtaagctg ctctgcgcgg atgaaacctg cggttatcgt atggatccgg
     4141 cagaaaaaga aaagtagaaa aagacaaata tcttaaaata atcgaaaact tcaaaaaact
     4201 cttgttattg ctttggaatt gtgctaaaat tcaattatta gtatagtata cctttgagat
     4261 aaggagggtt tcgattatga gcgttcaatt gttggacaaa acaagaaaaa tcaacaagct
     4321 attgcataac aacaattcac ataaggttgt ttttaatgac atctgcaaag ttttgagtga
     4381 gatcctgtta tccaatatcc ttgttatcag caggaagggc aaagtccttg gcgtaagtct
     4441 ctgcccaggt gtggatgaga tcgaggagct gatcgaagat caggtaggcg gctttgttga
     4501 caaaatgtta aatgaacgtc ttctcagtgt tttgtctaca aaggagaacg taaacctggc
     4561 aacgctgggc tttgcggagg agaacgtgaa aaagtatcag gccattatta cccccatcga
     4621 tattgcaggg gagcgtctgg gtacattatt tatttataaa tcagacagcc agtatgatat
     4681 tgatgatatc atcctcagcg aatacggcac tacggtggta ggacttgaga tgatgcgctc
     4741 tgtgaatgag gaaaatgcag aggagacaag aaaagttcag attgtaaagt ctgccatcag
     4801 caccctgtct ttctcggagc tggaagccat tatccatatt tttgaagagc ttaacggaaa
     4861 tgagggcatt ctggtagcca gcaagattgc agacagggtt ggtattaccc gctctgtgat
     4921 cgtaaatgca cttcgcaagt ttgaaagcgc cggtgtgatt gagtcacgtt cctccggcat
     4981 gaagggtacc tatatcaagg ttctcaacga cgtggtgttt gatgagttaa aggccattaa
     5041 ggcgtctaat tcaaatctga aataggattt tatcaaaaag ataaaggaca gtttgcaaaa
     5101 agtgcttgca agctgtcttt ttttatgata tagtagaaag gctgaaaaaa cacgtctgtg
     5161 gattttttgg aaaggtgcct ggggcagagc ctgaggtctt ctgaagaagc atgagaagca
     5221 ggcggacgat gccggaaccc atgctcagcg gttcggctaa aaaccaaacg gaggtataaa
     5281 catgagcgta atttctatga agcagttact ggaagctggt gtacacttcg gtcaccagac
     5341 aagaagatgg aaccctaaga tggctccata catctacaca gagagaaacg gcatctacat
     5401 cattgactta cagaagtctg taggaaaggt agatgaggct tacaaggctg tatctgatat
     5461 cgcagctgag ggcggcacta tcctgttcgt aggtacaaag aagcaggctc aggaagcaat
     5521 caagactgag gcagagcgct gcggtatgta ctatgtaaac gagagatggt taggcggtat
     5581 gctgaccaac ttcaagacca tccagagccg tattgcaaga ctgaaagaga tcgaggctat
     5641 gtctgaggac ggaacctttg atgttcttcc aaagaaggaa gtaatcgagt taaagaagga
     5701 atgggacaag ttagagaaga acctgggcgg aatcaaggat atgaagaagc ttccagacgc
     5761 aatcttcgta gtagatccca agaaggagca catctgtatt caggaggctc atactctggg
     5821 tatcaccctg ttaggtatcg ttgatacaaa ctgcgatcct gaagagttag attatgtgat
     5881 ccctggcaat gacgacgcta tcagagccgt taagctgatc gtttccaaga tggctgacgc
     5941 tgtgatcgag gctaagcagg gcgaagtttt agagggcgca atggagatcg aagtacctgc
     6001 agatgcattt gaagcagagg gtgcagaggc gtaattaaaa cttttaatat tggaggatga
     6061 caagatggca gtaaccgcag caatggtaaa agagttgaga gagatgaccg gtgccggaat
     6121 gatggactgc aagaaggctc ttgcagctac agacggcgat atggaaaagg ctgttgagtt
     6181 cttaagagaa aagggacttg caggcgcagc taagaaggct ggacgtattg cggctgaggg
     6241 tatcgtagat acattactga ctgctgatga gaagaccgca gtagtagtag aggttaatgc
     6301 tgagactgac ttcgttgcaa agaatgccgt attccaggca tacgttgcag acgtagcagc
     6361 acaggctctg gcttcttccg cagctgatat ggacagcttt atggctgaga agtgggctaa
     6421 ggatgagtcc ctgactgtaa aagaggctct ttcctctcag atttccatca tcggcgagaa
     6481 catgaatatc cgccgttttg agaaggtaac agaggagaac ggttttgttg cttcctacat
     6541 ccatgcaggc ggaaagatcg gcgttctggt tgacgttgag accaacgttg taaatgacga
     6601 gatcaaagag atggctagaa acgtagctat gcaggcagct gcattaaagc cgcagttcac
     6661 cagcagagat gaggttgacg aggctttcat cgagcatgag aagagcatcc tgatggctca
     6721 gatccagaat gatcctaaag aggcttccaa gcctgagaag gtaatccagg gaatgatcca
     6781 gggacgtatc aacaaagaat taaaggaatt ctgtctgtta gatcaggttt atgtaaaggc
     6841 tgaggatggc aagcagagtg tagctcagta tgttgcagct gttgcaaagg caaacaatgc
     6901 agacgttaag atcaagaagt tcgttcgttt cgagaccggc gagggtcttg agaagaagaa
     6961 tgaggatttt gcagctgagg ttgcggctca gatgggtaag taatttccgg aataaaatct
     7021 aggttaaaac ccttgaaaag ctcagaaatg ggcattttca agggtttttt gcggtttgaa
     7081 acggatttgc gctttacgat gcattaatct atagattata atgcaggtgt atatcaggat
     7141 ctggtttggc ctgtactgga gcagggttct gcatagacat ggctatgagc agattaccca
     7201 ggtgatggaa aagaacacag actaaaggta agacagaaac acgctaagga aggaaaa
//
LOCUS       DBMDUH010000137         7114 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_4497_length_7114_cov_4.087409, whole genome shotgun sequence.
ACCESSION   DBMDUH010000137 DBMDUH010000000
VERSION     DBMDUH010000137.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 7114)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7114)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7114
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4497_length_7114_cov_4.087409"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            129..235
                     /locus_tag="ACZPCG_10605"
                     /pseudo
     CDS             129..235
                     /locus_tag="ACZPCG_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148688.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            443..649
                     /locus_tag="ACZPCG_10610"
     CDS             443..649
                     /locus_tag="ACZPCG_10610"
                     /inference="COORDINATES: protein motif:HMM:NF024835.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1285542.1"
                     /translation="MKISYNKLQKLMADNQMKRSDLMRVAEFSPYAATKLNKNEPVSL
                     GVLMRICEVFHCDIGDICEVILEE"
     gene            684..932
                     /locus_tag="ACZPCG_10615"
     CDS             684..932
                     /locus_tag="ACZPCG_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285543.1"
                     /translation="MTNKELLQQIHRDNREIKRNFQHLANIGLMGVLGNFAAEAKEKG
                     DGEGKTLIRAGLFCIFISEILILISEINDCRKARTKEE"
     gene            1041..1769
                     /locus_tag="ACZPCG_10620"
     CDS             1041..1769
                     /locus_tag="ACZPCG_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR1285544.1"
                     /translation="MSGHSKFANIKHKKERNDAAKGKIFTVIGREIAVAVKEGGPDPA
                     NNSKLRDVIAKAKANNMPNDTIDRGIKKAAGDANSVNYETLTYEGYGPNGVAIIVDTL
                     TDNKNRTAANVRSAFTKGSGNVGTPGSVSYMFDKKGQIIIDKEECEMDPDELMMIALD
                     AGAEDFSEEEDSYEVITAPDDFSAVREALEAQNIPMMEADVTMIPQTWVELTDEDSIK
                     KMNRILDLLDEDDDVQAVYHNWDE"
     gene            complement(2047..3009)
                     /locus_tag="ACZPCG_10625"
     CDS             complement(2047..3009)
                     /locus_tag="ACZPCG_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HIR1285545.1"
                     /translation="MNNHPIYPYPDTLVSAAETRQEHTPVTPLPNPGEGGPVYDGSEP
                     AIPLPNPGEGGPVFDGAEPVIPLPNPGEGGPVFDGPGWNTNGHFHTHFHPPVFRPIFP
                     AFGSSITVIPGISFPCYNCTASGNFGRVRVLNASTGYQPFNVSLGGWMIAGQLANGDI
                     TSYVQAASGRRTMTISGANGYVYIQKSITIRPGASMTVAIINTASGLDLKEISDASCS
                     APSGSACLRTCNLSLNLGPFDVTLENSSGSYTPFTNVAYKNVTSYATVYPNWYQIYIS
                     RTGSFPGNAIAAASATLEANVSYTLYLFNAPTATEGLRAMIVSN"
     gene            3144..3419
                     /locus_tag="ACZPCG_10630"
     CDS             3144..3419
                     /locus_tag="ACZPCG_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HIR1285546.1"
                     /translation="MNEEKKRMAATALGLPKDVVLGDVLVRFTGFHEVCIENYRSILM
                     YTDTLVRIQARNCRVTLHGRELRIRYYNQDAMGVCGQIQSMEFEPLL"
     gene            3446..4669
                     /locus_tag="ACZPCG_10635"
     CDS             3446..4669
                     /locus_tag="ACZPCG_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HIR1285547.1"
                     /translation="MLLWLFRSIKGYVRICLKDGSPERFLNLCSARGFKIWNIVCRDK
                     GEYEAYMTVADVRRVKPLVRKSGVRLRITGRFGFPFFIFRNRKRWYYAAGLGTFFILL
                     YGMTLFIWDIEFEGNYRYTEDNLRICLEKMDLHCGILKKKINCEEVETFIRTDLPEVT
                     WVSASVTGTRLVVKIRENQVLSAVPQIDETPCDLVAGTSGVIRSMVVRSGIAQVSVGD
                     TVEKGQILVSGRVPVTGDNGDEITSHLVRADADIEAETVIEYEKEIPFSRMLRIPTGQ
                     TRKGIYVKAGPWSAAALFPALGKKEEWDYYMEEHQAVLFSDYCLPLYLGVIEGKRMIS
                     YEKAYTKQELALLAERKEKEEAEKLEEKGVQILENNDRIEKSVSGYRIKGEMVALGPI
                     SQCRPVEEISKESEE"
     gene            4677..5672
                     /locus_tag="ACZPCG_10640"
     CDS             4677..5672
                     /locus_tag="ACZPCG_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007711744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HIR1285548.1"
                     /translation="MSVIETMIDIPAEHEQNVCGQFDAYLKKIERTLHVTMIARNGEI
                     KIIGPEKAVSQARSVFNNLIQLSMRQNAITEQNVDYALSLSFTEEDGQILEIDKDIIC
                     RTITGKPVKPKTLGQKHYVDAIRKKMIVFGIGPAGTGKTYLAMAMAIQAFKNGEVGRI
                     ILTRPAIEAGEKLGFLPGDLQSKIDPYLRPLYDALYQIMGAESYLHNSEKGLIEVAPL
                     AYMRGRTLDNAYIILDEAQNTTPAQMKMFLTRIGFGSKVIITGDQTQKDLPSGAVSGL
                     DTALKVLKRIDDIGFCYLTSSDVVRHPLVQKIVQAYDDYEKKAEPKKRTPRDKGR"
     gene            5678..6175
                     /gene="ybeY"
                     /locus_tag="ACZPCG_10645"
     CDS             5678..6175
                     /gene="ybeY"
                     /locus_tag="ACZPCG_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HIR1285549.1"
                     /translation="MTISIEYEAEKKLELPYETIIKDVVEASLDYEKCPYEAEVNVIL
                     TDNETIREINRDHRQIDSATDVLSFPMVDYEAPSDFDHVEDAVEDYFNPETGELMLGD
                     IVISVEKVEEQAEKYGHSQTRELAFLVAHSMLHLCGYDHMEEEERLLMEERQRAILET
                     RGYTR"
     gene            6295..>7114
                     /locus_tag="ACZPCG_10650"
     CDS             6295..>7114
                     /locus_tag="ACZPCG_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HIR1285550.1"
                     /translation="MKTMNKKGAKRAADFVVQGSILAMAGILVRIIGMLYRMPLNDII
                     GKQGNGYYTSAYNVYNILLILSSYSMPVAISKMISSRLARGEYENCRRILRAGLLYAT
                     IAGGIAGCALWFGADLFAGLMKTPFSSYALKTLAPTIWIMAYLGVLRGYFQGTGTMVP
                     TAVSQILEQIVNAVVSVVAAGLLFQTGAAMNAARGAQNYSYALGAAGGTIGTGAGAAT
                     ALIFFVMLTLSKGREVLGEETVPVHTADGSGRAKAESYGRLMYVLTITVLPIIVS"
BASE COUNT         2072 a         1290 c         1981 g         1771 t
ORIGIN      
        1 attttcttgt ctgtatatct gaaaaagaaa tacaatacta aaacttcctg ccattgtttt
       61 aacaaaatag ttcattttct cacccccttt ttccaaggag aggactgaaa aagtaggtat
      121 aggtggggga gataatagaa gggcagctga tgttggctca tgtccatatg ttagtgagca
      181 tcccgccgat ataagtatat tgagttttat gggggatctg aagggaaaaa gcgcatggat
      241 tagcgctttg aggtgtggac aagaaggccg ggcttagagc ctgtgtcccg agttatccat
      301 tttacggata gcagcggata ttatggtaaa aagtgaaata aatcggctat attcattgaa
      361 aatttaacga tttttatata caatgttaaa tatgctattt tggcttggta ttgggacaaa
      421 aagtgaaaaa ggggtacgga agatgaagat aagttataat aagcttcaaa aattgatggc
      481 agataatcaa atgaaacgat ccgatttaat gagagttgcg gaattttcgc cgtatgcggc
      541 aacaaaatta aataaaaatg aaccggtttc tcttggggtg ctgatgcgaa tctgtgaggt
      601 atttcattgt gatattggag atatctgtga ggttatttta gaggagtaat tggatcagag
      661 gttaaaaaag aaaggtgata gatatgacaa acaaagaatt actacagcaa attcacagag
      721 acaacagaga aattaaaaga aattttcagc atctggccaa tattggactt atgggagtgc
      781 ttggaaactt tgctgcggag gcaaaggaaa agggcgatgg ggaaggaaaa acactcatta
      841 gagcagggct gttctgtatc ttcatatctg aaattctgat tttgatcagt gaaatcaatg
      901 actgcaggaa agccagaacg aaagaggaat gaaattgaaa atctgagcac aattttctgc
      961 ttgcaagatg aaatgaatcg tgataggata gaaaagatat cagtactgaa aaagaagata
     1021 aaagaaaggg tgttatacca atgtcaggac attcaaaatt tgccaacatt aagcataaaa
     1081 aggagcgcaa tgatgctgca aagggaaaaa tctttacagt tatcggccgt gaaattgcgg
     1141 ttgctgtaaa ggaaggcggt cctgatcctg ccaataacag taagctgcgt gatgtaattg
     1201 caaaggcaaa ggcaaacaat atgccaaatg ataccattga ccgcggtatc aaaaaggctg
     1261 ccggtgatgc caactctgta aactatgaaa ctctgaccta tgagggctat ggtcccaatg
     1321 gcgttgcaat tatcgtagat actctgaccg acaataaaaa tcgtacagca gcaaatgtaa
     1381 gaagtgcttt tacaaaggga agcggaaatg taggtactcc tggaagcgtg tcctatatgt
     1441 ttgataaaaa gggtcagatc attattgaca aggaagaatg tgagatggat ccagatgaat
     1501 tgatgatgat tgctctggat gcaggcgcag aggatttttc cgaagaggag gacagttatg
     1561 aggtgattac agccccggat gatttttctg ctgtccgtga ggcgctggag gctcagaata
     1621 tccccatgat ggaagccgat gtaacaatga tccctcagac ttgggtagag cttacagatg
     1681 aggattccat taagaagatg aaccgtattc tggatctgct tgatgaggat gatgacgttc
     1741 aggcggtgta ccacaactgg gatgaataat tatgcgaatg acttatttcc tgaaaaaggc
     1801 ttaaaatcta gcttttaggg taagggattc aagaaaatgc ataagagatt gcataaaatc
     1861 cgtatcagtg atgatatacg ggcaaatttt taaacccttc gagatgggtg tgcaaagacc
     1921 tctttttgtt tatcgtaatt gacagacaaa aggaggtctt ttttatcatc agctgccggc
     1981 ggtattacgg tgattaaaag gagtatgggc tgcacaccaa tttaagtgcg gcagcccttt
     2041 cagaggtcag tttgatacga tcatagcccg caggccttct gtagctgtag gagcattgaa
     2101 aagataaagc gtataggaaa cattggcttc cagggtggca gaagcagcgg ctatcgcatt
     2161 tccggggaag gaaccggtac gggagatata gatttggtac cagttgggat aaacggtagc
     2221 atatgaggtc acatttttat aagcaacatt cgtaaatggt gtataagagc cgctgctgtt
     2281 ttcaagggtc acgtcaaagg gtcccagatt cagggacaga ttgcaggttc tgaggcaggc
     2341 ggaaccggat ggtgcgctgc aggaagcatc ggagatttcc ttgagatcca gtccggaagc
     2401 agtgttgata atggcaaccg tcatagaggc tccgggacgg atggtaatag atttttggat
     2461 ataaacatat ccgttggcac cagaaatggt catggtgcgc cgtccggagg ctgcctgaac
     2521 gtaactggtg atatctccgt tggccagctg tcctgcgatc atccagccgc ccagggatac
     2581 attaaagggc tgatatccgg ttgaggcgtt aagtacccgc acccgtccga aattgccgga
     2641 agcagtacag ttgtagcagg ggaagctgat gccgggaatt acggtaatgg aggagccgaa
     2701 agcaggaaag atgggacgga atacaggagg gtggaaatgg gtatggaaat gaccattggt
     2761 attccagccc ggtccgtcaa agaccggtcc gccttctccg ggatttggca aagggataac
     2821 aggctctgcc ccatcaaaga ccgggcctcc ctcgccggga tttggcaggg gaatggctgg
     2881 ttctgagccg tcataaaccg ggcctccctc gccgggattt ggcagaggcg ttacaggtgt
     2941 atgttcctga cgggtttcag cagcgcttac cagggtgtca gggtatgggt aaatgggatg
     3001 gttattcatg gatacctccc ggttgattgt tcattacagt gtatgctgtg ctgctgcctg
     3061 gtgtgcaaag ggctgccgga ggtattttcg gatgctgaaa gccatatact gttacagtga
     3121 acgatcagga gggagaacag agcatgaatg aagaaaaaaa gcgcatggca gcaacggctc
     3181 tggggcttcc caaggatgtg gttttgggag atgtactggt tcgttttacc ggttttcacg
     3241 aagtttgtat tgaaaattac agaagcatcc ttatgtatac agatactttg gtgaggatcc
     3301 aggcaagaaa ctgccgtgta actctccacg gccgggaact gaggatccgg tattacaatc
     3361 aggatgcaat gggggtatgc ggtcagatcc aatcgatgga atttgaaccg ctcctgtagg
     3421 aaacatataa aaggagggag agcctttgct tttgtggctg tttcgcagca taaagggata
     3481 tgtacggatc tgtttaaaag acggctcgcc ggaacgtttt ttgaatcttt gcagtgcaag
     3541 agggtttaaa atctggaaca tcgtctgccg tgataaggga gaatacgaag catatatgac
     3601 tgtggcggat gtcaggaggg taaagcctct tgtgagaaaa tccggtgtcc gcttaaggat
     3661 cacagggcgc ttcggttttc ctttttttat tttcagaaac aggaaacgct ggtattatgc
     3721 agccggactg ggaacttttt tcatactgtt gtatggtatg actcttttta tctgggatat
     3781 tgaatttgaa gggaattaca ggtacacaga ggataatctc aggatctgtc tggaaaagat
     3841 ggatctgcat tgcggtattt taaagaagaa gataaactgt gaggaagtgg agacatttat
     3901 acgaacggat cttccagagg ttacctgggt atcggccagt gtgacaggaa cccgtctggt
     3961 agtaaagatc agggaaaatc aggttttatc tgctgtgccc cagatagatg aaacaccctg
     4021 tgatctggtg gcagggacgt ccggagtgat tcgctctatg gtagtcagaa gcggtattgc
     4081 tcaggtatct gtaggagata cggtagagaa aggacagata ttagtcagcg gccgtgttcc
     4141 tgttacggga gataacggag atgagataac atcccatctg gttcgggcag atgcagatat
     4201 cgaagcggaa acagtgattg agtatgaaaa agagatccca ttttcaagaa tgctgcggat
     4261 tcccaccgga cagacgagaa aggggattta cgtaaaggcg ggcccatggt ctgctgctgc
     4321 gctttttcct gctttaggga aaaaagagga gtgggattat tatatggagg agcaccaggc
     4381 agtactgttt tctgattact gtcttccgct ttatctggga gtgatagagg gaaaacggat
     4441 gatttcctat gaaaaagcct atacgaaaca ggagttggct cttctggctg aacgaaaaga
     4501 aaaggaggaa gcggaaaaat tagaggaaaa gggggtacaa attcttgaaa ataatgatag
     4561 aatagaaaag agtgtatccg gataccggat caagggggag atggtggcgt tagggcctat
     4621 cagtcaatgc cggccggtgg aggaaatctc taaagagtca gaggagtgaa gaccgaatga
     4681 gtgtaattga aacaatgatc gatattcccg cagaacatga acagaatgtc tgcggtcagt
     4741 tcgatgcgta cttaaagaaa atagagcgca cccttcatgt gacgatgatc gcaaggaacg
     4801 gcgagattaa gatcatcggg ccggaaaagg cggtatccca ggcgagaagt gtatttaaca
     4861 atctgatcca gctttccatg cgtcaaaatg ccattacaga gcagaatgta gattacgctc
     4921 tgtcattatc atttacagaa gaagacggac agattttgga gattgataag gatatcatct
     4981 gccgaaccat tacgggaaag ccggtaaagc ccaaaacgct tggacagaag cattacgtgg
     5041 atgccatacg caaaaagatg atcgtctttg gaatcggccc ggcgggaacg ggaaagacgt
     5101 atcttgctat ggctatggcg atccaggcct ttaaaaacgg agaggttggg cggatcatcc
     5161 ttacacgtcc ggcgattgag gcgggggaga agctgggatt tttgccggga gatcttcaga
     5221 gcaagatcga tccctatctg aggcctcttt atgatgctct gtatcagatt atgggagcgg
     5281 agagttatct gcacaattca gaaaaggggc tgattgaggt ggcgccatta gcttatatga
     5341 gaggacgcac cctggataat gcctatatta tcctggatga agcccaaaat accactccgg
     5401 cccagatgaa aatgttcctg acccgtatcg gcttcggctc caaggttatt atcacaggtg
     5461 atcagaccca gaaggatctg ccgtcaggag ctgtgtccgg actggatacg gccttaaaag
     5521 tattaaaacg cattgatgac atcggctttt gctatcttac cagttcggat gtggtgcgcc
     5581 atccgctggt tcagaagatt gtacaggctt atgatgacta tgagaagaaa gcggagccta
     5641 aaaagaggac tcccagagat aaaggaaggt aaatacgatg accatttcca ttgaatatga
     5701 ggcagaaaag aagctggagc ttccctatga aaccattata aaggatgtgg tggaagcgtc
     5761 actggactat gaaaaatgcc cttatgaggc agaggtaaat gtaatcctta cggataatga
     5821 gaccatccgg gagatcaacc gggatcacag gcagattgat tcggctacgg atgtgctgtc
     5881 ctttcctatg gtagactatg aggctccttc agattttgac catgtagagg atgcggtgga
     5941 ggattatttt aatccggaga caggagaact gatgctggga gatattgtga tctccgtaga
     6001 gaaggtagaa gagcaggctg aaaagtacgg ccattcccag accagggagc tggcattttt
     6061 agtggctcac agcatgcttc acctctgcgg ttacgatcat atggaggaag aggaacgtct
     6121 gctgatggag gaacgccaga gagcgatcct ggaaaccagg gggtataccc gctgatgcag
     6181 aaagcaggaa tgtgcctgtt ttatctatga cgcttctgac ggtctctatc acagatacca
     6241 gggactttga tcggatcgtg cttaagtgag atacgaaaaa ggagcaaaaa acagatgaaa
     6301 acaatgaata aaaagggagc caaaagagct gcagattttg tggttcaggg cagtattctg
     6361 gctatggcag gcatcctggt gcggatcatc gggatgcttt accgtatgcc cttaaatgat
     6421 attattggaa agcagggaaa tggatattat acctcagcgt ataatgtata caatattctg
     6481 cttatccttt cttcctacag tatgccggtg gctatttcca agatgatttc ttcacgcctt
     6541 gcaaggggag agtatgagaa ctgccgcagg atcctgcggg cgggtctttt gtatgccacc
     6601 attgcgggcg gaattgcagg ctgtgcactg tggtttggag cagatttatt tgcagggctg
     6661 atgaaaacgc ctttttccag ctatgcctta aaaaccctgg cgccgacgat ctggatcatg
     6721 gcctatctgg gagttttaag aggttatttt cagggaacag gcaccatggt gccaacggcg
     6781 gtgtctcaga ttctggaaca gattgtcaat gcagtggtaa gcgtagtggc cgccggcctt
     6841 ttgtttcaga caggagcagc tatgaatgca gcgagagggg cgcagaatta ttcctatgcg
     6901 ttaggagcgg caggaggaac tataggaaca ggagcaggag cagctacggc ccttattttt
     6961 tttgtgatgc tgactctttc aaaagggcgg gaagtactgg gagaggaaac ggttccggtg
     7021 cacaccgcgg atggctcagg cagggcaaag gcggaaagct acggccgtct tatgtatgtg
     7081 ctgacgatca cagttcttcc gatcattgta agca
//
LOCUS       DBMDUH010000138         6932 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_4624_length_6932_cov_3.877910, whole genome shotgun sequence.
ACCESSION   DBMDUH010000138 DBMDUH010000000
VERSION     DBMDUH010000138.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 6932)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6932)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6932
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4624_length_6932_cov_3.877910"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            30..350
                     /locus_tag="ACZPCG_10655"
     CDS             30..350
                     /locus_tag="ACZPCG_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HIR1285551.1"
                     /translation="MADEKNLNEEEEMTVTLTLDDDTEVECVVLTIFTAGERDYIALL
                     PMEGEDSEEGEVYLYRYSETEDGTPVLDNIEDDDEYEIVADAFDEMLDDQEYDELVGE
                     DEVE"
     gene            555..1235
                     /locus_tag="ACZPCG_10660"
     CDS             555..1235
                     /locus_tag="ACZPCG_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1285552.1"
                     /translation="MNGLKILVVDDEARMRKLVKDFLANKGFYVEEAGDGEEAVDKFF
                     AQKDIALVLLDVMMPKMDGWEVLKTIRKYSQVPVIMLTARSEERDELQGFSLGVDEYI
                     SKPFSPKILVARVEAILRRSNASGGDALSVGGILIDKAAHQVTIDGKEVELSFKEFEL
                     LSYFVENQGIALSREKILNNVWNYDYFGDARTIDTHVKKLRSKMGDKGDYIKTIWGMG
                     YKFEVSGQ"
     gene            1232..2755
                     /locus_tag="ACZPCG_10665"
     CDS             1232..2755
                     /locus_tag="ACZPCG_10665"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1285553.1"
                     /translation="MKRSIRFRFTTLFIGMLAAVLLAIWVINSFFLEQYYINDKVKIL
                     QDAYEQLNDCAVEQALEGRNIIDDLPSFYSKDGEQTEVSRMLRVMNEQYNTTVVLIDS
                     LNDRTFPTFWDNRLLSDLVRQYILGRSMPDTEAIISEENYKIEKRYDKHNNGYYLQNW
                     GFFDDNRTIFIMSMPIASIHESVALSNRFMIYVGSAALVLGAVFMYFAGKRITSPILS
                     LAALSERMSELDFDARYTADSQDEIGVLGRSMNTLSEKLKETIGALKTANNELQQDIE
                     EKIRIDEARRDFIANVSHELKTPIALIQGYAEGLTEGMAEDPESRDYYCEVIMDEAGK
                     MNKMVKQLLTLTALEFGNDKPVIEQFDLTGLIHGVLSSASILLQQKEAEVEFEPSGPV
                     MVWADEFKIEEVITNYLNNAMNHLNGKRKIKIRLEKHENEVTVFVFNTGNHIPEEDLD
                     KLWTKFYKVDKAHTREYGGSGIGLSIVKAIMDSHHKACGVRNAEEGVEFWFTLDCSL"
     gene            2888..4183
                     /gene="guaA"
                     /locus_tag="ACZPCG_10670"
     CDS             2888..4183
                     /gene="guaA"
                     /locus_tag="ACZPCG_10670"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HIR1285554.1"
                     /translation="MKQDMIVILDLGSTENTVLAREIRAMGVYSEIYPHDITAEELKA
                     LPNVKGVVIYGGPNHIVDGVRIDVNEEIYSAGYPVMSIDHEPAKCQEAYGTWEEALPH
                     LRAFVFDTCHAEANWNMKNFVEDQIELIRRQVGDKKVLLALSGGVDSSVVAALLIRAI
                     GKQLVCVHVNHGLMRKGESESVIEVFKNQMDANLIYVDATERYLTKLEGVADPEQKRK
                     IIGAEFIRIFEEEARKLEGIEFLGQGTIYPDIVESGTKTAKVVKSHHNVGGLPEDLQF
                     KLVEPLRQLFKDEVRQCGLELGLPDEMVFRQPFPGPGLGVRCLGAITRERLEAVRESD
                     AILREEFHNAGLDKKVWQYFTVVPDFKSVGVRDNARSFEYPVIIRAVNTIDAMSATIE
                     QLEWPVLLKITDRILKEVKNVNRVCYDLSPKPTATIEWE"
     gene            4382..4972
                     /locus_tag="ACZPCG_10675"
     CDS             4382..4972
                     /locus_tag="ACZPCG_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003502574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HIR1285555.1"
                     /translation="MKKDYSKLWVLFKSMFALSACTFGGGFVIVSLMKKRYIEELKWL
                     EEDEMLDVTAITQSAPGPLPVNASVIIGYRIAGIIGSLTAILGTILPPMIIISVISLF
                     YEQFRTNPYINTALQVMRAGVAAVIFDVVINLAANVVHTRRSLYIGMMMIAFIATYVL
                     DVSAMIIILTCLGIGLIDLMIQTTKNKKGEMRNVIG"
     gene            4959..5531
                     /locus_tag="ACZPCG_10680"
     CDS             4959..5531
                     /locus_tag="ACZPCG_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HIR1285556.1"
                     /translation="MLLAKLFIAFIQVGLFSVGGGYAAIPLIQEQIVNIYGLMTLSEF
                     SDLITVAEMTPGPISINSATFVGMRIAGIPGVLLCTIGCIIPSFCICLLLAHFYYKYR
                     NVDGVQVVLGSMRPAVVALIASAGASILMLGLFQAELYNVEFNNFRFIEFSIFAGSLF
                     ILRRYKTSAIAIILGSGMIGTLIYVLLGAV"
     gene            5547..6419
                     /locus_tag="ACZPCG_10685"
     CDS             5547..6419
                     /locus_tag="ACZPCG_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006778576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1285557.1"
                     /translation="MTLRHLMIFTAVVDEGGMNKAAKTLHLSQPNISQAIAELEQHYG
                     VKLFERLSQRLYLTKEGELLLSFSRHILDSYKQMEEVMNQAVEKTQIQIGCSVSVGTC
                     LINRILDKAERVLPKCEFQVKVTNSSEIEQALLTNQIDIGMIEGVVSDENLIAAPMCK
                     DELVIVCGKAHPFAKKESISLKALEGQNYVSRESGSTERNQYEQFLEQKGIRLKRIFC
                     STNTEAIKNAVVHGRGIAIFSKRMIEKEAVEGSLIIVPVEDIHIIRDFHFIRHKNKYM
                     SKTLQIMQDICTDL"
BASE COUNT         2100 a         1197 c         1732 g         1903 t
ORIGIN      
        1 cagatcatat ctgagaaagg aaacccaata tggcagacga gaagaattta aatgaagaag
       61 aggaaatgac agttaccctt accctggatg atgacaccga agtggaatgt gtcgttctta
      121 caatttttac agcaggcgag agggattata ttgctcttct tcctatggag ggagaggact
      181 ctgaggaagg cgaagtatac ctgtaccgct acagcgagac agaggacgga actcccgttc
      241 tggacaacat cgaggacgat gatgagtacg agatcgtagc agatgctttt gacgagatgt
      301 tagacgatca ggagtacgat gagctggtag gagaggacga ggtagaatag gctcctttcc
      361 tgaagttaaa gtttcatagc aacggattta aaagactgtc ctggctgaac aaaacggccg
      421 gacagtcttt tttgttatgg tgatgaggaa aatggctgtc tgaacctgtg aaacttatgt
      481 gaaaacgtag aaatgccctg ttttttctgc taaaatggta tcatcatcag atatggaaac
      541 aggaggagcc ggccatgaac ggattaaaaa tactggtagt agatgacgag gccagaatgc
      601 gcaagcttgt aaaagacttt cttgcgaaca aggggtttta tgtggaggaa gcaggagacg
      661 gggaagaggc cgttgataaa ttttttgccc agaaggatat tgctttggtg cttttagatg
      721 taatgatgcc gaaaatggac ggttgggagg tattaaagac catacgcaaa tattcccagg
      781 ttccggttat catgctcaca gccagaagcg aggaacggga cgagcttcag ggcttttctc
      841 tgggcgtgga cgaatacatt tcaaagccat tcagtcctaa aatcctggtg gcccgcgtgg
      901 aggcgattct gcgcagatcc aatgcctccg gaggagatgc tttaagcgtt ggaggtattc
      961 tgattgataa ggcggcccat caggtaacga tagacggcaa agaggtggag ttaagcttta
     1021 aggagtttga gcttctttcc tattttgtag aaaatcaggg aattgctctt tccagagaga
     1081 agattttaaa taatgtgtgg aattatgact attttggtga tgccagaacc attgataccc
     1141 atgtgaaaaa gcttcgcagc aagatgggcg ataaaggcga ttatatcaag accatctggg
     1201 gaatgggata taaatttgag gtgagcggac aatgaagcgt tccatccgtt ttcgttttac
     1261 gacgctgttt atcgggatgc tggcagccgt cctgcttgca atctgggtga tcaacagctt
     1321 ttttctggag cagtactata tcaatgataa ggtgaaaatc cttcaggatg cctatgaaca
     1381 gctcaatgac tgtgcggtag agcaggcgct ggaagggaga aatattattg atgacctgcc
     1441 aagtttctat tccaaggacg gagaacagac cgaggtaagc cggatgctgc gtgttatgaa
     1501 tgaacagtac aatacaacgg tggtgctgat tgacagctta aatgaccgta cttttcccac
     1561 attctgggat aacaggcttt tatccgatct ggtccgtcag tatattttag gcagatcaat
     1621 gccggataca gaagccatta tttctgagga aaattataaa atagaaaaaa gatatgataa
     1681 acataacaat ggatattatc tgcagaactg gggctttttt gatgacaaca gaaccatttt
     1741 tattatgtcc atgcctattg ccagtatcca tgagagtgtg gccctgtcga atcgttttat
     1801 gatctatgtg ggaagcgcag cccttgtgct gggagccgta tttatgtatt ttgcgggaaa
     1861 aaggattact tcgcctattt tgtcccttgc agccttatct gaacggatgt ccgagctgga
     1921 ttttgatgcc agatatacgg cggattctca ggatgagatc ggtgtgctgg gccggagtat
     1981 gaataccctt tcggaaaagc tgaaggaaac cataggggct ttaaaaacag ccaataatga
     2041 gcttcagcag gacattgagg aaaagatacg gattgacgag gcacgcaggg attttattgc
     2101 caatgtatcc catgagctta agacgcctat tgcattgatt cagggatatg cggagggcct
     2161 tacagaaggc atggcagagg acccggagag tcgtgattat tactgcgagg tgatcatgga
     2221 tgaggccggg aaaatgaaca agatggtaaa gcagcttctt accctgaccg ccctggagtt
     2281 tggaaatgac aagcccgtca tcgagcagtt tgaccttaca gggctgattc atggagtgct
     2341 ttcctccgca tccattctgc ttcagcaaaa agaggcagag gtggagtttg agccttcagg
     2401 gccggtgatg gtatgggcgg atgaatttaa gattgaagaa gtcataacca attatctgaa
     2461 taatgccatg aatcatttga atggaaaacg aaagataaaa atccgtttgg aaaagcatga
     2521 aaatgaagtg actgtctttg tctttaatac aggaaaccac attccagagg aggatctgga
     2581 taagctctgg acaaaatttt ataaggttga taaagcccat accagagaat atggagggag
     2641 cggcattggc ctttccattg tgaaagccat tatggattcc caccataagg cctgcggggt
     2701 cagaaatgca gaggaaggcg tagagttctg gtttacctta gactgcagcc tgtagcaggc
     2761 atcttgtgaa aaacagagaa gcattatttg cgtatttcgt caaagtttta cttgtataac
     2821 agcatggaat cctgtatgct gtagttatag aaattttaaa tcatgtgatg acacaggagg
     2881 aagatttatg aaacaggaca tgattgtcat tttggatctg ggaagtacag agaatactgt
     2941 gcttgccaga gaaattcgtg ccatgggtgt atacagcgag atttatcccc atgacattac
     3001 ggcagaagag ttaaaggcac ttccaaatgt aaaaggtgtt gtgatctacg gaggaccaaa
     3061 ccatatcgta gacggcgtac gcattgatgt aaatgaggaa atctacagcg ccggttatcc
     3121 ggttatgtcc attgaccatg agcctgcaaa gtgtcaggaa gcttatggca cctgggaaga
     3181 agctcttcct cacctgaggg cctttgtgtt tgatacctgt catgcagagg caaattggaa
     3241 tatgaagaac ttcgtagagg atcagattga attgatccgc cgtcaggtag gtgacaagaa
     3301 ggttctttta gctctttcag gcggtgttga cagttctgtt gtggcagctc ttttgatcag
     3361 agctatcggc aagcagcttg tctgcgtgca tgtaaatcac ggcctgatga gaaaaggcga
     3421 gtcagagagt gtgatcgagg tatttaaaaa tcagatggat gcaaacctga tctatgtaga
     3481 tgcgacagag cgttatctca caaagctgga gggcgtagcc gatccggaac agaagagaaa
     3541 gatcatcggc gccgagttta tccgtatttt tgaggaagaa gcaagaaagc tggagggaat
     3601 cgaatttctg ggtcagggaa ccatttaccc ggatatcgta gagagcggaa ccaagactgc
     3661 caaggttgta aagtcccacc acaacgtagg aggactcccg gaggatctgc agtttaagct
     3721 ggttgagcct ctgcgccagc tttttaagga cgaggttcgt cagtgtggtc tggagctggg
     3781 acttcctgat gagatggtgt tccgtcagcc attcccgggt cctggtctgg gtgtacgctg
     3841 tctgggagca attacaagag aacgtctgga ggctgtccga gaatccgacg ctattttacg
     3901 tgaagaattc cacaatgccg gactggacaa gaaggtatgg cagtacttta ccgtagtgcc
     3961 ggatttcaag tctgtcggag tccgcgacaa tgcaagaagc ttcgagtatc ctgtgatcat
     4021 ccgtgctgtc aacaccatag acgccatgag cgccaccatc gagcagctgg agtggccggt
     4081 actcttaaag attacagacc gcattttgaa agaagtgaaa aatgtaaacc gtgtctgcta
     4141 tgatttgagc ccgaagccta cggctacgat tgagtgggaa taaaataaaa aggagccgtg
     4201 aaacctgaaa aatcaaaggt ttcacggctc ctttttttgt gggcggaact aaaatggaac
     4261 agaaaaacac agcatatttc ctagttatat attttatata taagttttat aaaaactatg
     4321 tattttacat ataattaatt cgatgataca atccaagtgt caaacaaaag gaggaaatat
     4381 aatgaaaaaa gattacagta agctgtgggt tttatttaag tcaatgtttg cattgagtgc
     4441 atgtaccttt ggcggaggat ttgttattgt ttctttaatg aagaaaagat atattgaaga
     4501 attaaaatgg ttagaagaag atgagatgtt agatgtaact gcaattacac aatccgctcc
     4561 cggtcctttg ccggtaaatg catctgtcat aatcggatat cggattgcag gcattatagg
     4621 ttctttgaca gcgattttag gaaccatcct tccgcctatg atcatcattt ctgttatttc
     4681 tttattctat gagcaatttc gtacgaatcc atatatcaat actgcattac aggtcatgag
     4741 ggccggagtt gcagctgtca tatttgatgt tgtgatcaac ttagcggcaa atgttgtcca
     4801 tacaagacgc agcctgtata taggcatgat gatgattgcg tttattgcaa catatgtatt
     4861 ggatgtcagc gctatgatta tcattcttac atgtcttgga atcggactga ttgatctgat
     4921 gattcaaaca acaaaaaaca aaaaggggga aatgagaaat gttattggct aaactgttta
     4981 ttgcgtttat tcaggttggt ttattcagtg tgggaggcgg ttatgctgca attccgttga
     5041 ttcaggagca gattgtaaat atttatggat tgatgacctt aagtgagttt tcagatctta
     5101 ttacagttgc agaaatgact ccgggtccga tttccatcaa ttccgcaact tttgtaggta
     5161 tgcggattgc aggaattccg ggtgtgctgc tgtgtacgat tggatgtatc attccatcat
     5221 tttgtatttg cttactatta gcccattttt attataaata tagaaatgta gacggggtac
     5281 aggttgtgtt gggctccatg cggccggcag tagttgcgct gattgcttct gccggagctt
     5341 caatcttaat gctggggctt tttcaggcag aactttataa tgttgagttc aataattttc
     5401 gttttataga attttcgatt ttcgccggtt ccttatttat actgcgaagg tataaaacaa
     5461 gcgccattgc aattattctg ggaagtggta tgataggtac gttgatatat gtattattag
     5521 gcgctgttta aaaggaaagg atttttatga ccttacgaca cttaatgatt ttcactgcag
     5581 ttgtagatga aggcgggatg aataaggccg caaaaacact gcatctttct cagccgaata
     5641 tcagtcaggc tatcgcagag ctggagcagc attatggcgt gaaattgttt gaacgtcttt
     5701 cgcaaagatt atatctgaca aaagaaggag agctgttgct ttccttttca cgccatattt
     5761 tagattccta taaacaaatg gaagaagtga tgaatcaggc ggtggaaaaa acacagatac
     5821 aaataggctg cagtgtttct gtggggacgt gtcttattaa ccgtatttta gataaggctg
     5881 aaagggttct tccgaaatgt gaatttcaag tgaaggttac aaattcatcg gaaattgagc
     5941 aggcattatt aacgaatcag attgatattg gtatgattga gggagttgtt tcagatgaaa
     6001 atttaatagc ggctcccatg tgtaaagatg aacttgtcat tgtgtgcgga aaagcacatc
     6061 catttgcaaa gaaagaaagt atttctctaa aggcgttaga gggacaaaac tacgtcagca
     6121 gagaaagcgg aagtacagag cggaatcagt atgagcagtt cttagaacaa aaaggaatta
     6181 ggctgaaacg aattttctgc agtacaaata cggaggcaat aaaaaatgca gtagtacatg
     6241 gcagaggaat cgcaatattt tcaaaacgaa tgattgaaaa agaagcagta gagggaagtt
     6301 tgattatagt gcctgtagag gatattcata taatcagaga ttttcatttt attagacata
     6361 aaaataaata tatgtcgaaa acattgcaga ttatgcagga tatctgtact gatttataat
     6421 ggaacggcag gttttatatc cggatatccg gatgataatc tgaagataaa gaattttgta
     6481 acagttcagc attgcatctt cttgcccact tacagcggac aagaagcgtc gggcgtccgc
     6541 cctatgaagc ttcaggcaga aaaattctta tgaaagcaag ctttcaacgc ttttttctgc
     6601 ctgaaacttc gcatggctga actgttacaa gaattttgaa aaaacttaaa aaaactgttg
     6661 acatttggcg atacatctgg taagataatc ttcgctgtcg ggaacgacag aaacacaaaa
     6721 gaaacgacat taaaaaatgt caaaataaat ttaaaaaagt tattgacaaa cgaaaaataa
     6781 tcggttataa taaataaccg ttgcggacga agagtcacac aacagcaaag ttccttgaaa
     6841 attgaagatt aaacagtatg taaaaccctg aaaattctaa caattcaaaa ggtttggttt
     6901 agaccttagg atttgagaaa attcagaaca aa
//
LOCUS       DBMDUH010000139         6895 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_4646_length_6895_cov_5.193848, whole genome shotgun sequence.
ACCESSION   DBMDUH010000139 DBMDUH010000000
VERSION     DBMDUH010000139.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 6895)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6895)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6895
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4646_length_6895_cov_5.193848"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1283)
                     /gene="kdpB"
                     /locus_tag="ACZPCG_10690"
     CDS             complement(<1..1283)
                     /gene="kdpB"
                     /locus_tag="ACZPCG_10690"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HIR1285558.1"
                     /translation="MAREKHTEFITKDILKSSVKGAFQKLDPRYMIKNPVMFVVEVGF
                     FITLLLSIFPSMFGDGGELRIYNVIVTLILFVTILFANFAESVAEGRGKAQAETLKKT
                     KKDTTARILDKAGNETVISASQLKKGDVVLVEAGELIPNDGEVIEGIASVDESAITGE
                     SAPVTREAGGDFSSVTGGTTVVSDWLKIRITSEPGQSFLDKMISLVEGASRQKTPNEI
                     ALNTLLVSLTIIFLIVVVTLHCFADYSQTRIPISTLIALLVCLIPTTIGGLLSAIGIA
                     GMDRVTRFNVIAMSGKAVEACGDVDTMILDKTGTITYGNRLAAEFYPVKGVLKEDLID
                     YAVMTSLKDTTPEGKSVVELGNTMGTRIDPSAASQMEFVEFTAQTKMSGVNLSNGMEV
                     RKGASEAIKARVRELNGTIPKDLEEIVTGISKLGGT"
     gene            complement(1312..3039)
                     /gene="kdpA"
                     /locus_tag="ACZPCG_10695"
     CDS             complement(1312..3039)
                     /gene="kdpA"
                     /locus_tag="ACZPCG_10695"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HIR1285559.1"
                     /translation="MLQIMVALVIYMVLVIPMGTYLYHITTGRKTFADPVFDRIDNGI
                     YRICKIDRQGMNWKQYAVSLLTTNAVMIFAGYLILRLQGSLFLNPNGTGAMEPTLAFN
                     TIISFMTNTNLQHYAGESGLSYLSQTLVIIFMMFTSAATGYAACMAFIRGITGTGKDM
                     GNFYEDMIRIMTRVLIPISIVIALILVSQGTPENYTANLTVQTLEGKNQDIAMGPMAA
                     LVAIKHLGTNGGGFLGANSSTPLENPTILSNMVEMYAMAILPGACVIVFGRMFHDKNK
                     KAQAEKKGTRPIFGRQAGPVFGAMAILFLVGLMICFFAEKAGNPVLEQAGLSQAMGNM
                     EGKEVRFGISQSALFTTVTTSFTTGTVNNMHDSLTPLGGMVPLLHMMLNCVFGGKGVG
                     FMNMIMYAILAVFLCGLMIGRTPEYLGKKIEGREMKLIVIVLILHPLLILGFSALAVV
                     NQAGLAGITNPGFHGLSQVLYEFASSAANNGSGFEGLADNTGFWNMTTGIVMFLGRYI
                     PIIAQIAIAGSLLAKRRMNESVGTLRTDNVLFMLILVFIVYIFAALTFFPALALGPIA
                     EHLILWYPM"
     gene            complement(3091..3183)
                     /gene="kdpF"
                     /locus_tag="ACZPCG_10700"
     CDS             complement(3091..3183)
                     /gene="kdpF"
                     /locus_tag="ACZPCG_10700"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0043462 - regulation of ATP-dependent
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HIR1285560.1"
                     /translation="MDMAGMIFLGLVLAGLAGYLVYALVYPEKL"
     gene            complement(3228..3353)
                     /locus_tag="ACZPCG_10705"
     CDS             complement(3228..3353)
                     /locus_tag="ACZPCG_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285561.1"
                     /translation="MMDVLVMDALMIGTLLAGFGLLRLFLGWCIDQTEGTGKKNQ"
     gene            complement(3450..3644)
                     /locus_tag="ACZPCG_10710"
     CDS             complement(3450..3644)
                     /locus_tag="ACZPCG_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285562.1"
                     /translation="MNTGRGAFGRIEAAFDRRAEKFTFRHPYLTILIMFIAVPFGMLA
                     AVSALTFLAAFPAALLLGLV"
     gene            complement(3822..4739)
                     /gene="metA"
                     /locus_tag="ACZPCG_10715"
     CDS             complement(3822..4739)
                     /gene="metA"
                     /locus_tag="ACZPCG_10715"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004414 - homoserine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase MetA"
                     /protein_id="HIR1285563.1"
                     /translation="MPIKVQRDLPAKAILESENIFIMDEDRAMRQDIRPLEILILNLM
                     PLKEETETQLLRALSNTPLQVDCTFLMLSTHVSKNTSASHLNKFYVTFESIRRKKFDG
                     MIITGAPVENLEYEEVNYWEELSVIMEWSKTHVTSTLHICWGAQAGLYYHYGIPKYSR
                     ESKLSGIYSHRVLDRKVPLVRSLNDTFLAPHSRYTEVRREDIRKHPELMILAESDEAG
                     VFLVMSRDGRQIFVQGHPEYDRMTLNNEYHRDLKKGLDPALPCNYYENNDPFSIPALT
                     WRNTSNTLYTNWLNFYVYQITPYLLDCEE"
     gene            complement(4756..6708)
                     /gene="ligA"
                     /locus_tag="ACZPCG_10720"
     CDS             complement(4756..6708)
                     /gene="ligA"
                     /locus_tag="ACZPCG_10720"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HIR1285564.1"
                     /translation="METKIQRMKELIPVLKEAGKAYYQESREIMSNFEYDRLYDELTS
                     LEEETGVILSGSPTQQVGYEVLSELPKETHEAPMLSLDKTKSTEDLQEWLGSQKGLLS
                     WKMDGLTIVLTYENGSLIKAVTRGNGEIGEVITANARAFSNIPLSIAYKGQLVLRGEA
                     VITYSDFEKINSQIGDADAKYKNPRNLCSGSVRQLNSQITAERNVHFEAFALVRAEGV
                     DFKNSRKCQFEWLKSQGFEVVHYEEVTAENLPEKIEWFGKETEHNDIPSDGLVLLYDD
                     IAYGESLGRTAKFPRNAIAFKWQDEVRETTLSYIEWSASRTGLINPVAVFEPVELEGT
                     TVSRASVHNISIMESLELGEGDRITVYKANMIIPQIADNLTRSGVKHIPEHCPVCGGP
                     TKIRQINDVKSLYCTNPDCQAKKIKSFTLFVSRDALNIDGLSEATLEKFISMGFIHEY
                     ADMFHLEAHKDAIVEMEGFGQKSYDNLIASVKKASQTTLPRVIYGLGIAGIGLANAKM
                     LCRHFRYDFKAMRNSGLEELTAVDGIGEVLARAWMDYFADKKNNDMVDRLLEELSIEE
                     EPVQEGEKPFEGLTFVITGSVEHFANRKELQELIESKGGKASGSVTSKTAYLINNDTT
                     SNSSKNKKAKELGIPIISEKEFLNML"
BASE COUNT         1706 a         1836 c         1446 g         1907 t
ORIGIN      
        1 gtaccgccca gcttggaaat tcctgttaca atttcttcca gatctttagg gatggttcca
       61 tttaattccc ttactctggc cttgatggcc tcggaggcgc cctttcttac ctccataccg
      121 tttgaaagat taacgccgct catcttggtc tgagccgtaa actccacaaa ttccatctga
      181 ctggctgccg aagggtcgat cctggttccc atggtatttc ccagttctac taccgatttt
      241 ccttccggag tggtatcctt taatgaggtc atcaccgcat aatcgatcag gtcttccttt
      301 aaaacaccct ttaccggata aaactccgca gccagacggt ttccatatgt aatggttccc
      361 gtcttatcaa ggatcatggt atctacgtct ccgcaggcct ctacggcctt accgctcatg
      421 gcaattacat taaaccgggt cacacggtcc attcccgcaa taccgatagc agaaagaagg
      481 ccgccgatgg tagttgggat cagacatacc agaagggcaa tcagggttga aatggggatc
      541 cgggtctgag aataatctgc aaagcagtgc agcgtcacta ccacaatgag gaaaataatg
      601 gttaagctta caagaagggt atttaaagcg atctcattgg gcgtcttctg tctggacgcg
      661 ccctctacaa gtgaaatcat cttatctaag aaggactggc ctggttctga ggtgatccgg
      721 atctttaacc agtctgatac caccgtagtt ccgcctgtga cagaagaaaa atcaccgcct
      781 gcctctcttg tgacaggggc agattcaccg gtaatggccg attcatccac agaagcaatt
      841 ccttcaatca cctctccgtc attggggatc agctctcctg cttctactaa tactacatct
      901 cccttcttta actgggaagc cgagatcacc gtttcgttcc ctgccttatc cagaatccgg
      961 gccgtggtat cctttttcgt cttttttaac gtctctgcct gagcttttcc acggccctct
     1021 gcaaccgatt ctgcaaagtt tgcaaacagg attgtcacaa acaggatcag cgttacgatc
     1081 acattataaa tccgaagctc accgccgtct ccaaacatgg aaggaaaaat gcttaacagc
     1141 agggtaatga aaaatccgac ctccaccaca aacatcaccg gattctttat catatatctg
     1201 ggatccagct tctgaaaggc ccctttaacg gagcttttca gaatatcctt tgtaataaat
     1261 tcagtatgtt tctctcttgc catagaaatt cctttctacc tgtttccctg cctacatggg
     1321 gtaccacagg atcagatgct ctgcgattgg tcccagagcc agagccggga agaaggtcag
     1381 agccgcaaaa atatatacga taaacacaag aatcagcata aagagcacat tgtccgtgcg
     1441 cagcgtaccc acgctttcat tcatgcggcg ctttgcaaga aggcttcctg caatggcgat
     1501 ctgggcaata ataggaatat agcgccctaa aaacattaca atgccggttg tcatattcca
     1561 gaatcccgta ttgtccgcca gcccctcaaa gccggagccg ttgtttgccg ctgaagaggc
     1621 aaattcatag agcacctggc ttaagccgtg aaagcccgga ttggtgattc ctgcaagtcc
     1681 cgcctggttt accactgcca gagcggaaaa gcccaggatt aaaagaggat gaaggatcag
     1741 tacgatcacg atcagcttca tttccctgcc ttcaatcttc tttcccagat attccggcgt
     1801 acggccgatc ataagtccgc agaggaaaac tgccaggatc gcatacatga tcatgttcat
     1861 aaatccgacg cccttgccgc cgaatacgca gttaagcatc atgtgcagca gcggcaccat
     1921 accgcctaag ggagtgaggg aatcatgcat attgttgacc gtaccggtgg taaaggaggt
     1981 ggtaacggtg gtaaagagcg ccgactggct gattccaaac cgtacctctt tgccttccat
     2041 atttcccatg gcctgactta aacctgcctg ctccagaacc ggatttccgg ccttttcagc
     2101 aaaaaagcag atcataagcc ccactaaaaa cagaattgcc atggctccga atacagggcc
     2161 tgcctgacgg ccgaatatgg gacgggtgcc cttcttttct gcctgcgctt ttttattctt
     2221 atcatggaac atacgtccaa agacgatcac acaggctccg ggcagaattg ccattgcata
     2281 catttctacc atattggaaa gaatggtcgg gttctccaga ggagtcgatg aattggcccc
     2341 caaaaagccg cctccgttgg ttcccagatg cttaatggca accagcgctg ccatcggtcc
     2401 catggcgata tcctgatttt ttccctctaa cgtctgcacc gtcagattgg ctgtataatt
     2461 ttccggtgtt ccctggctta caaggatcag tgcgatcaca atggaaatgg ggatcagcac
     2521 acgggtcatg atacggatca tatcctcata gaagtttccc atatcctttc ccgttccggt
     2581 tattccgcgg ataaaggcca tgcaggccgc atatcccgtg gccgccgagg taaacatcat
     2641 aaaaatgatt accagcgtct ggcttaaata ggaaagaccc gactctccgg catagtgctg
     2701 aaggtttgta tttgtcataa agctgatgat cgtattaaag gcaagagtcg gttccatggc
     2761 tcctgtccca ttgggattta aaaacaaact tccctgaaga cgcaggatca aatatcccgc
     2821 aaagatcatt accgcattgg ttgtcagcag acttaccgca tactgtttcc agttcatgcc
     2881 ctgtctgtca attttacaga ttctgtaaat tccgttatcg atcctgtcaa atacaggatc
     2941 cgcaaatgtt ttccggcctg tggttatgtg atacaggtac gttcccatgg gaatcaccag
     3001 aaccatatag atcacaagcg ccaccataat ctgcagcatc gcttttcttc ctcctgtctt
     3061 tcatttggcg tatccgaaag gaaaccgcgc ctagagtttt tctgggtata ctagagcata
     3121 caccagatat ccggcaagtc ctgccaggac aagtcctaaa aaaatcattc ccgccatatc
     3181 catttcctcc ttattacctg cattctcatt tgatcaaacc attccgctta ctggttcttt
     3241 tttccggttc cctctgtctg gtcgatgcac catccgagaa aaagtctcag cagtccaaag
     3301 cctgccagca gagtacctat cattaaagca tccattacca aaacatccat cattgtcttt
     3361 cctcctttcg gtattcaaga gattatcaca gttataataa aataggcatt aagcatccga
     3421 ccctctctgt taagattcca taaatgcttt tataccagtc caagcagcag cgctgccgga
     3481 aaagccgcta aaaaagtcag ggccgatacg gctgccagca tcccaaatgg cactgcgata
     3541 aacatgatca gtatggtaag atagggatga cgaaatgtaa atttctcggc ccggcggtca
     3601 aaggcagcct ctattctgcc aaatgctcct cttcctgtat tcataatgcc tgcctccttc
     3661 ccggtcatgg atcaggaaga cgttttcctg tcctgtgtat gtatcatagc atgggaggga
     3721 ttaagagggt aaaagggtca gatggcggag gattaagatt ttgttaggat aaaaaatgac
     3781 acccgccatt gcagcgagtg ccatttttta tacttatctt cctactcctc acaatccagc
     3841 aaatacggcg taatctggta cacataaaag ttcagccagt tcgtatacag cgtattggac
     3901 gtatttctcc aggtcagcgc cggaatggaa aagggatcat tattttcata gtaattacag
     3961 ggaagagcag gatccagccc tttcttcaaa tccctgtggt attcattatt cagcgtcatg
     4021 cggtcatatt ccggatggcc ctggacaaaa atctgccggc catcccggct cataaccagg
     4081 aatacgccgg cctcatcgga ctctgccagg atcatcagtt ccggatgctt ccttatgtct
     4141 tctctgcgga cctccgtata ccgggagtgc ggcgctaaaa aggtgtcatt taagcttctc
     4201 acaagaggaa ccttccgatc cagaactcta tggctgtaga tccctgacag cttgctctcc
     4261 ctgctgtatt tgggaatccc atagtgataa taaagcccag cctgagcccc ccagcagata
     4321 tgaagggtgg aggtgacatg agtcttgctc cactccatga taacagacag ctcctcccag
     4381 taattcactt cctcatattc cagattctcc accggcgcac ctgtgatgat cattccgtca
     4441 aatttcttcc tgcggatgct ttcaaaggta acataaaatt tatttaaatg actggccgaa
     4501 gtattttttg aaacgtgggt ggacagcatg agaaaggtgc agtccacctg caggggagta
     4561 ttggaaagag ccctcagaag ctgggtttcc gtttcttcct ttaagggcat caggtttaaa
     4621 atcaagatct ccaaaggccg gatatcctgc ctcatagctc tgtcttcatc cataataaat
     4681 atattttctg attccaggat tgcctttgcg ggcagatccc tctgtacttt gatcggcata
     4741 taaaccctct ccctcctaga gcatgttcag gaattctttt tctgaaataa tcggaatccc
     4801 cagctctttt gcctttttat tttttgatga attagatgtg gtgtcattat tgatgagata
     4861 tgcggttttg gaggttacgg agccggatgc tttgccgccc ttgctttcga tcagctcctg
     4921 gagctccttg cggttggcaa aatgctccac cgatccggta attacaaagg taagcccttc
     4981 aaagggcttt tctccctcct gcaccggctc ctcttctatg gacagctcct ctaacagccg
     5041 gtctaccata tcattatttt tcttatctgc aaaataatcc atccacgccc gggccagcac
     5101 ctctccgatc ccgtccacag ccgtcagctc ctccagaccg ctgtttcgca tggctttaaa
     5161 atcatatcgg aaatgacggc agagcatctt tgcatttgcc aggccgattc ctgcgattcc
     5221 caatccgtag atcactctgg gaagggtcgt ttgagaagct ttcttcacgg aagcaatcaa
     5281 attgtcatag gacttctgtc caaagccctc catctctacg atcgcatctt tatgggcctc
     5341 cagatggaac atatccgcat attcgtggat aaagcccatg ctgataaatt tttccagagt
     5401 tgcctctgaa agtccgtcaa tatttaatgc atccctgctt acaaacaggg taaagctttt
     5461 gattttcttt gcctggcagt ccggattggt acaatacaga ctctttacat cattgatctg
     5521 ccggatcttt gtaggacccc cgcagaccgg acaatgctcc ggaatatgct tcacgccgct
     5581 gcgggtcaga ttatccgcaa tctgtggaat gatcatattg gccttataga cggtaatcct
     5641 atccccttct cccagctcaa ggctctccat aatgctgata ttgtgaacgc tggccctgct
     5701 caccgtagtt ccctccagtt ccacaggttc aaacacagcc accggattga tcagacccgt
     5761 tctggaggcg ctccactcga tataggaaag ggtggtttcc cgaacctcat cctgccactt
     5821 aaatgcgatg gcattgcgcg gaaatttcgc cgtgcgtccc agtgactctc cgtatgcaat
     5881 atcatcatag agaagaacca ggccgtctga agggatatca ttgtgctctg tctccttccc
     5941 aaaccactcg atcttctcag ggagattttc tgctgtcact tcctcatagt gaaccacttc
     6001 aaagccctgg ctctttagcc attcaaactg gcattttctg gaatttttaa agtcaactcc
     6061 ctcagcgcgc accagtgcaa atgcctcaaa atgtacattt ctttctgctg taatctgact
     6121 gttgagctgg cgtacggagc cgctgcacag atttctgggg ttcttgtatt tggcgtctgc
     6181 atccccgatc tggctgttaa tcttttcaaa atccgaatat gtgatcacag cttcgcccct
     6241 gagcaccagc tgccccttat aggcaatact taaagggata ttggaaaatg cccttgcatt
     6301 ggctgtgatc acctccccga tctctccgtt tcctctggtt actgccttga ttaaacttcc
     6361 gttttcatag gtcagtacaa tggtaagccc gtccattttc caggaaagaa ggcctttctg
     6421 gctgccaagc cactcctgta aatcctctgt gctttttgtc ttatcaagcg acagcatagg
     6481 tgcctcatgt gtttccttgg gaagctcgct taaaacctca tagcctacct gctgggtagg
     6541 gcttcccgac aaaatcaccc ctgtttcctc ttccagtgat gtcagctcat catagagcct
     6601 gtcatattca aaattgctca taatctcccg gctctcctga tagtaagcct ttccggcttc
     6661 cttaaggacg ggaatcaatt ccttcattct ctggattttc gtttccattc acttctcctg
     6721 tctgtttcgc attatttatt tttaagtctt aaatctcttt cggacaaact ttttgactgt
     6781 tcaagctgcg ccagaagcgt cctgtattcc tctcctgtta tttccctgaa agagccttcc
     6841 tttaagttcc ccagatgaat attcataatc cgcacccgtt tcagagtgcg cacat
//
LOCUS       DBMDUH010000140         6869 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_4670_length_6869_cov_6.196809, whole genome shotgun sequence.
ACCESSION   DBMDUH010000140 DBMDUH010000000
VERSION     DBMDUH010000140.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 6869)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6869)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6869
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4670_length_6869_cov_6.196809"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            327..602
                     /gene="spoIIID"
                     /locus_tag="ACZPCG_10725"
     CDS             327..602
                     /gene="spoIIID"
                     /locus_tag="ACZPCG_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007719085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HIR1285565.1"
                     /translation="MKEYIEERAIAIANYIIDHNATVRQTARRFGISKSTVHKDVTDR
                     LEHINPSLAAQARVVLDINKSERHIRGGLATREKYQHRLAICSKENS"
     gene            complement(693..1490)
                     /gene="ucpA"
                     /locus_tag="ACZPCG_10730"
     CDS             complement(693..1490)
                     /gene="ucpA"
                     /locus_tag="ACZPCG_10730"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009429448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HIR1285566.1"
                     /translation="MAKLDGKVALITGAAAGLGEGISTAYAKYGAKLIMVDLSPAVEE
                     TAEKLRKEYNSEIITVVANVADRAQMDAAAAKGIEAFGEINVACCNAGVCRLAPFEEM
                     DDATRDFHIDVNIKGVWNTCKAVIPYMLKQGGGNIVIASSVTGDIVADAGEAAYAMTK
                     AALVGLTKCLAVEYANRNIRVNCSQLGYARTPMVEKMALESNPENPEAAIADIAVGVP
                     MKRLAKPIEVGELFAFLGSDESSYLTGSQIVIDGGSTLPETMSIGTN"
     gene            complement(1563..2969)
                     /locus_tag="ACZPCG_10735"
     CDS             complement(1563..2969)
                     /locus_tag="ACZPCG_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR1285567.1"
                     /translation="MEDKQNKSNGLNKSLKLIFVYTVATGSIFTFVNYWDSVFYGYCG
                     SGTFLAFALMTVAILPVALVYSELASLFHTSGGELIYNTVGINKHVGFLASWLIMAAW
                     ISVPPAVVMAIMTWVNKTLGLNLGTWPMVGCAAVLLVLYFLMSIQNVQFLVKAQAGML
                     FCNITVTILTGFILLFSGHWHLENFGNIFTTTLESVNGIPGWIIGMALLITPYFGFET
                     VPQMVEEGDFPIKNSKKAICGSILTCGCIYVFYYFCVAGLDGLKDIHANFAQDGFLTI
                     ATMQNVLGWTFWPIIVGILSCLLGMGASLLGFWMSTVRMLYSMANKNFLPKVFAKVNK
                     NQQPMLPNIFLLVLSLIFIVLQNSSDFMSAFFNLMSFGCACAYALTMISAVCIHHKHP
                     EWKSANTVKGGDAFRVFAMLIAVAIAFFCTLGQGIDSWICFGVYLGIGVVIWLWMLLV
                     NWKKHPVEIETPDGVQQF"
     gene            complement(3189..3761)
                     /locus_tag="ACZPCG_10740"
     CDS             complement(3189..3761)
                     /locus_tag="ACZPCG_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing response regulator"
                     /protein_id="HIR1285568.1"
                     /translation="MYRVVVADDDGKACKKAVEFLQNNGYQVVGEAADGLEAVMVCRR
                     ERPDFVIMDLSMPVMTGLEAIRVIHREKLAGFIIVLTSCRNKALLHQAAQEDIMGYLV
                     KPVEESALLGAVEVALCQEDHIMKAERECEKTKKALEDRKYIEKAKGLLMEHRKLSEN
                     EAYSYIRRQAMDKEQTMAEIARILLMAYNM"
     gene            complement(3951..4283)
                     /locus_tag="ACZPCG_10745"
     CDS             complement(3951..4283)
                     /locus_tag="ACZPCG_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285569.1"
                     /translation="MRKRWNVLLIAGAVMALAVGCGAKEETKSVQETSAVSETARTEG
                     TTAHTEETEKAPEAEKKESEVQAFHAADETARSPSMAGFVLTGENGGPNIVFGMRDGK
                     LAVLGINY"
     gene            4582..5289
                     /gene="pnuC"
                     /locus_tag="ACZPCG_10750"
     CDS             4582..5289
                     /gene="pnuC"
                     /locus_tag="ACZPCG_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016296800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HIR1285570.1"
                     /translation="MKSFTTLKNYFSKTELILWSTSVVLILIAFCLFDRTSYMTLWTS
                     LIGVTSLIFCAKGNPMGQLLMVLFSLLYGMISLNVSYYGEMITYLGMTMPMAVFSLIT
                     WLKNPFNGNRSEVEIYHIKRHDIVLMSVLTAVVTILFYFILKAFNTANLIPSTISVTT
                     SFLAVYLTFKRSPYYAAGYAANDLILIILWTMASMEDISCLSVTVCFAAFFVNDIYGF
                     LNWRRMGQRQAAVAAGS"
     gene            complement(5302..6078)
                     /locus_tag="ACZPCG_10755"
     CDS             complement(5302..6078)
                     /locus_tag="ACZPCG_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR1285571.1"
                     /translation="MTVILSNFGVGGLVGLTGVAGFLLPIVYTGLLQMSVTQGLALSF
                     AAFILSGALGSFNYKKAGNLDVSFGLRLSAGSMAGAVLGVKLNLIIPEEQVKILLYVV
                     VLLSGISILLRKDGKAAETEKKAAAGAAGAKEKGFRLEKHLVPTLLLGFVTGLICALS
                     GAGGPILVMPLLVVFGTGVRVAVGIALFNSIFIGIPACIGYMSQCDVPALLPVLAAAL
                     ASHGVGVYLGSKNAVRINQGVLKRGIAVFSILIAVWKLFL"
     gene            6430..>6869
                     /locus_tag="ACZPCG_10760"
     CDS             6430..>6869
                     /locus_tag="ACZPCG_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
                     /protein_id="HIR1285572.1"
                     /translation="MTNYKPKDFAGLLGVSVKTLQRWDREGILKANRTPTDRRYYTYD
                     QYLQFKGIHTENDDRQTVIYARVSTRNQTDNLQNQAAFLRQFCNARGMIVDQCIEEYG
                     SGLNYNRKKWNQLLDEVMEQKIRTIIVTHKDRCCHSLGLKELLG"
BASE COUNT         1967 a         1711 c         1288 g         1903 t
ORIGIN      
        1 gattttgaca tttgattcct cattatttaa agacatattt tataagtata ccattttttc
       61 agggctcttg caatattttc aatgcagaaa acagcaaatt ccagttcatc ggtatttttt
      121 atttttatat cattcaacat cgtcagctcc cctccgtcat tcatattttt gatctcagca
      181 tcttgaggtt aaagtaaata cttctgtata cttttaattt ccatggaaca agtatatgac
      241 aaccttctgc ttttggctat ctccccaatg cataaaatac cacagcttgt aatatgatgg
      301 taaaaacgtt ctcagggtga aataccatga aggaatatat tgaagaacgg gccattgcta
      361 ttgcgaatta cattatcgac cacaacgcta ccgtgcgcca gacagccaga cggtttggaa
      421 tttccaagtc tacggtacat aaggatgtca ccgatcgttt ggaacacatc aacccatccc
      481 tcgccgccca agccagagta gtgctggaca tcaacaaatc cgaacggcat atccggggcg
      541 gactggctac aagggaaaaa taccagcatc ggctggcaat ctgcagtaaa gagaacagct
      601 aaacaaatac ttatgtgtac tatatacatc acaaaaaccg gcagaccctc acaatctgcc
      661 ggtttttata tcaattattc taatttcaaa gcttagttgg ttccaatcga cattgtctca
      721 ggcaatgtgc ttccgccatc aataacaatc tgagaacctg tcagatagct ggattcgtcg
      781 cttcccagga atgcaaagag ctctcctacc tcgattggtt ttgcgaggcg cttcatggga
      841 acgccgactg cgatatctgc gattgctgcc tccggatttt caggattaga ttccagagcc
      901 atcttttcaa ccataggtgt acgcgcatag cccaactgtg agcagtttac acggatgttg
      961 cggtttgcat actcaacagc cagacatttt gtcaggccaa ccagagcagc ctttgtcata
     1021 gcatacgctg cctcacctgc gtctgctaca atatctccgg ttacagagga agcgatcaca
     1081 atattgcctc cgccctgctt taacatatat gggattactg ccttacaggt gttccataca
     1141 cctttgatat ttacatcgat atggaaatct ctggtagcat catccatctc ctcaaaagga
     1201 gccagacggc atacacctgc gttgcagcag gctacattga tctctccaaa agcctcaatg
     1261 ccctttgccg cagctgcatc catctgtgcg cggtccgcta cattggcaac tacggtgata
     1321 atctctgaat tgtattcctt gcgcagtttt tcagctgttt cttctacagc aggagagaga
     1381 tctaccatga tcagctttgc tccgtatttt gcatacgcag tagaaattcc ttctccaagt
     1441 ccggcagcag ctccggtaat cagtgcaact tttccatcca gtttagccat tttatcttct
     1501 cctttacttc attcatttat tttcagacgt ttcgtttcgt ccggattaac tgttttacaa
     1561 acttaaaatt gctgtactcc atccggtgtt tcaatctcaa ccggatgttt cttccagttt
     1621 accagaagca tccacagcca gatcactaca ccgatgccca aatatactcc aaagcagatc
     1681 caggaatcaa ttccctggcc caatgtacag aaaaatgcaa tcgcaactgc aatgagcatc
     1741 gcaaacacac gaaatgcatc accgccctta acggtattcg cactcttcca ctcaggatgc
     1801 ttgtgatgaa tgcacacagc tgaaatcatt gttaaagcat acgcacaggc gcagccaaaa
     1861 ctcatcagat tgaaaaatgc actcataaaa tcggaagagt tctgaagtac aataaagatc
     1921 aaagataata ccagcagaaa aatgttagga agcatcggct gctgattttt atttaccttg
     1981 gcaaatactt ttggaaggaa attcttattt gccatggaat acagcatacg tacggtagac
     2041 atccagaatc ccagcaaaga tgctcccata cccagaaggc aggaaagaat acctacaata
     2101 atcggccaga aggtccatcc caatacattc tgcatggtcg caatggtcag gaaaccgtcc
     2161 tgtgcgaaat tggcatgaat gtccttcaga ccatccagac ctgctacaca gaaataataa
     2221 aatacataaa tacagccgca ggtgagaatg gaaccgcaga ttgctttttt agaattttta
     2281 atagggaaat ctccttcttc taccatctga ggaacggttt caaatccaaa atacggagtg
     2341 atcaaaagag ccattccaat gatccagccc ggtataccgt tcacagactc caacgttgta
     2401 gtgaaaatat ttccaaagtt ttccagatgc caatgaccgg aaaacagaag aataaatcca
     2461 gtcagaatgg ttactgtgat attacaaaac agcatacctg cctgagcctt taccagaaac
     2521 tgcacattct ggatactcat taaaaaatac agaaccaaga gtacagctgc acatcccacc
     2581 atgggccaag taccaaggtt taaacccagc gttttgttta cccaggtcat aattgccata
     2641 accactgccg gaggaacgga aatccacgct gccataataa gccaggaagc caggaaacca
     2701 acgtgcttat tgatccctac ggtattgtag atcagctcgc ctcctgaggt atggaacaag
     2761 gatgccagct cactgtatac cagagctacc ggaaggatcg ccactgtcat cagggcgaat
     2821 gccagaaacg taccgctgcc gcaataacca taaaatacag aatcccaata gtttacaaat
     2881 gtgaaaatag atcctgtcgc aaccgtataa acaaaaatca atttcagact cttattcaaa
     2941 ccattgcttt tattttgttt gtcctccatg taaaacacaa cccctttact tttactttgc
     3001 aatttttcag catacaaaaa agcccaaaat cccccctata cttaaaagag aaaatctgga
     3061 cttcgatgtc gctgttcgct gatatgcgcc gttgtatatc agttatgttt tttaaagaga
     3121 tatcgcttta attgctttca atcataccac tttgctcaaa gtttgtcaag taattaacaa
     3181 tttttatcct acatgttata cgccattaac aaaatccggg caatttctgc catggtctgc
     3241 tccttatcca tagcctgacg gcgaatatag gaatatgcct cattttcaga aagcttcctg
     3301 tgctccatta aaagcccctt cgccttttca atgtattttc tgtcctccag cgcttttttt
     3361 gttttctcac attccctttc tgccttcata atatgatctt cctgacaaag agctacctcc
     3421 accgctccca aaagcgctga ttcctctacc ggcttcacaa gatatcccat aatatcctcc
     3481 tgggctgcct gatgcagcag cgctttattg cggcaggaag taagcacaat gataaaacct
     3541 gccagcttct ccctgtggat cacccgaatt gcctcaagcc cggtcataac aggcatactc
     3601 aaatccataa tcacaaaatc cggacgttcc ctgcggcata ccataactgc ttccaggccg
     3661 tctgctgctt ctcccacaac ctgataccca ttattctgaa gaaattccac tgccttttta
     3721 caggctttac catcatcatc tgcaaccaca acccgataca taattccccc ttcatccaat
     3781 actccaaact taaaactgga aatttttgtc tatttattaa caattttcac tatgtgaaat
     3841 cattgtatca tggggggaat aaaattgcaa cttaaggcag ccggtatcac tgcataaagc
     3901 ctgcaacagc ctggttcaga tgaatcaaac gcctctggat ccgttcatct ttaatagtta
     3961 atccccaaca ccgccagttt cccgtctctc atcccaaata caatattggg cccgccgttt
     4021 tctccagtaa ggacaaaacc agccatactt ggggagcgtg ccgtctcatc tgccgcgtga
     4081 aaagcctgta cttcagattc ctttttctca gcttcaggag ctttttccgt ttcttctgta
     4141 tgtgctgtcg ttccttccgt cctggctgtt tctgatacag cgcttgtctc ctgtacgctt
     4201 tttgtttctt cctttgctcc gcatccaaca gccagtgcca ttacagctcc tgcgatcaat
     4261 aatacattcc atctcttcct catatttcaa ttctccttcc cagtttattt tacaattttc
     4321 ttttgttttg caaatttcag attagtttca gggttttaaa aatttttctt gatttttctc
     4381 aaaagtgccc gtatactgta tcacgaagcg gacgccgcag gagcgcccca tacgtttcag
     4441 acagcaaact gctgtgcata agatcaaaaa ttatctgcca ccgggtagga atgttgaaag
     4501 cattcacata cgcatcgata ggtctttgaa gatgtgtata tgaatgcttt ttatatatca
     4561 ccggcagata aggaggacat catgaaatca tttaccacac tcaaaaacta cttttcaaaa
     4621 acagagctga ttctctggag tacctcagtc gttttgattc tgattgcttt ctgtctcttt
     4681 gaccggacca gttatatgac cctttggacc tcattgatcg gagtgacttc tctcatcttc
     4741 tgtgccaagg gaaatcccat gggacagctt ttgatggtgc tgttcagcct gctttacgga
     4801 atgatttccc tgaacgtttc ttattatgga gaaatgatca catatctggg aatgaccatg
     4861 cctatggctg tgttctctct gatcacatgg ctgaaaaatc catttaatgg aaatcgttcg
     4921 gaagtcgaaa tctaccatat caaacggcat gatattgtgc ttatgtccgt tttgactgca
     4981 gtcgtgacca tcctgtttta ctttatttta aaggccttta acactgccaa tttaatcccc
     5041 agcacgattt ctgtaactac cagcttcctg gctgtatatc taacctttaa gcgcagtccc
     5101 tactatgcgg caggatacgc cgccaatgac ctgatcctga tcatactgtg gactatggcc
     5161 tcaatggaag atatcagctg cctgtctgta accgtatgct ttgccgcctt ttttgtaaat
     5221 gatatttacg gctttttaaa ctggcgcagg atgggacagc gacaggctgc ggtggctgca
     5281 ggcagctaaa aagctgtccc cctaaaggaa cagcttccag actgcaatca atatagaaaa
     5341 tactgcgatc ccccgtttta aaacgccctg atttatgcgc actgcgtttt tgctccccag
     5401 atacacgcca acgccatggc ttgccagagc ggcagccaac acaggcagga gcgccgggac
     5461 atcgcactgg ctcatgtagc cgatacaggc cggaatcccg ataaaaatgg agttaaacag
     5521 ggcaattcct actgccacac gaacgccggt gccgaatact actaaaagcg gcatcaccaa
     5581 tattgggccg cctgctccgg acaaagcaca gatcaaaccc gtaacaaacc ccagaagcag
     5641 ggtggggaca agatgcttct ccagtcgaaa gcccttctcc tttgccccgg cggccccggc
     5701 agccgccttt ttctccgtct cagcagcttt tccgtccttt ctcagcagaa tggaaatacc
     5761 ggacaacagc accaccacat acagaagtat cttgacctgc tcttccggga taatcaaatt
     5821 cagctttacg cccagaaccg cacctgccat acttcctgca cttaagcgca gaccaaagga
     5881 cacgtccaga tttccagctt ttttataatt gaaagatccc agcgcgcccg acaggataaa
     5941 ggccgcaaag ctcagtgcaa gtccctgggt gacgctcatc tgcagaaggc ctgtatacac
     6001 gatcggaagg agaaatcccg ccactccggt aagccctacc agaccgccga ctccgaaatt
     6061 ggataatata actgtcaacc agaaacagaa aaccttagag gattaaattt ttcgttaaaa
     6121 gccatatcaa acctgcctga gcgcagcgag tttttaatat ggcttttgct tttagaaaaa
     6181 tttcagcccc ttaggttttc ttatggccgg aaaccggagt cctgaacacc agacgccaga
     6241 gtgcggtgta aagtgcggaa actcaaagtc aattttcctc ttgtttccct tcccgaaaac
     6301 agttataatt ctctttactg cccattttca ggctaaaaag aggaaatatc aagtaatcaa
     6361 aatcattgat attagtgctt aaaagtgttt gaatatactc gaggaggatt atttatgaac
     6421 acttctaata tcactaatta caaaccaaaa gattttgctg gattattagg tgtctctgtt
     6481 aaaacgctgc agcgttggga tagagagggg attctgaaag caaaccgtac tccaactgat
     6541 agacgttatt acacttatga ccaatatctt caatttaaag gcattcatac cgaaaatgat
     6601 gatcgtcaga ctgttattta tgccagagta tctacaagaa accaaacgga taatctacaa
     6661 aaccaggcag cgtttttacg tcagttttgt aacgccaggg gaatgatcgt agaccaatgc
     6721 attgaagagt atggaagtgg tcttaattac aaccgtaaaa agtggaatca attattggat
     6781 gaagtgatgg aacaaaaaat caggacgatt atagtcacgc ataaagatag atgttgccat
     6841 tcattaggct taaaagaatt gcttggatt
//
LOCUS       DBMDUH010000141         6804 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_4716_length_6804_cov_4.072206, whole genome shotgun sequence.
ACCESSION   DBMDUH010000141 DBMDUH010000000
VERSION     DBMDUH010000141.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 6804)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6804)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6804
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4716_length_6804_cov_4.072206"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(156..1022)
                     /locus_tag="ACZPCG_10765"
     CDS             complement(156..1022)
                     /locus_tag="ACZPCG_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007712120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HIR1285573.1"
                     /translation="MSKEKTEVTVDRILSSKNGRWPVFIKLFVLVAAAGIISEIIGTK
                     TIPLGSVGKVNITAMVFAMLIAVLVTPDALGKIFSPLKSICSEKEVELSEAALSFMLL
                     ILGVKLGTSSGPNILKILQAGPALILQEFGNLGTMLVGLPIAMLLGMKREAFGATVSI
                     CREPSLGLINERYGANSPEFLGCMGTYMVGNLFGAVFFGVLASLAVNTGLHPYSLAMA
                     CGMGSGSMMTASSASLAATVPEMAEEIMAYASMSNICSNIDGLYMECFIAIPVAERLY
                     RVYSKLLRRKDA"
     gene            complement(1061..1888)
                     /locus_tag="ACZPCG_10770"
     CDS             complement(1061..1888)
                     /locus_tag="ACZPCG_10770"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007712122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HIR1285574.1"
                     /translation="MTKLKAAMLQSHVYSDKAENIRALCVNLEELAKEKPDLVTLPEM
                     FNCPYQSSNFPVYAEKEGEETWQALSSLAAKYGIYLSAGSVPETDEQGHIFNTAYVFD
                     RNGKQIAKHRKMHLFDIEVEGGQSFKESDTLTPGDQVTVFDTEFGKMGICICFDFRFP
                     ELARLMALEGARVILVPAAFNMTTGPAHWEILFRTRALDNQVYTLGTAPARDADAGYT
                     SWGHTIAVSPWGDIMGQMEEREGFMIQTLDLDKVKKVRQELPLLKNRRSDIYMLKKQ"
     gene            complement(1928..3034)
                     /locus_tag="ACZPCG_10775"
     CDS             complement(1928..3034)
                     /locus_tag="ACZPCG_10775"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR1285575.1"
                     /translation="MNYMELDTPALIIDREIMMDNLRFMQDYADRKKVALRPHTKTHK
                     MPKLALIQEDLGAKGITVAKVGEAEVMAAAGLKNIFIANEIVGAVKLARIRKLAESID
                     ISFGLDSIDQAKMIEAAFEGADKKAQVLIEIEVGEERSGVIEQDDYIKLLHYLKTCKN
                     IYLKGIFSHDGNSYGAADISECRNIHLTAQKRTLEFARLAKEEGMELETVSIGSTPSM
                     MHDFPILEGVTEIRPGTYIFMDASQANAYGSLDRNAATVLTTIISRPTAERVITDVGA
                     KGITAQTRSKGFCATQGLGLIKGWPDVEIFDVYDEHAIIYNQQFRDHVKVGDKVEIIP
                     NHICPVVNLHEYAYLVSKGQVIEKISVECRGKLL"
     gene            complement(3041..3433)
                     /locus_tag="ACZPCG_10780"
     CDS             complement(3041..3433)
                     /locus_tag="ACZPCG_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007712126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR1285576.1"
                     /translation="MNKPEVIETKYSVRDGGHYVPGIKYNGVLYISGQLSIDPEIGKV
                     PEGGVKAEAAQALKNLHTVLKEAGLNKEDVLQCRVYLPDVAYWGEFNQVYSEFFGAHK
                     PARVVVPTNHLYGGCLVEIEAVAACGKE"
     gene            complement(3860..5218)
                     /locus_tag="ACZPCG_10785"
     CDS             complement(3860..5218)
                     /locus_tag="ACZPCG_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HIR1285577.1"
                     /translation="MEREKFSSRLGFILISAGCAIGLGNVWRFPYIVGKYGGAAFVLI
                     YLAFLLVLGLPIVVMEFAVGRASKKSAALSFDLLEPKGSKWHLTKYIAMAGNYMLMMF
                     YTTVAGWMLLYFFKMLRGDFNGLDSAGTAEEFSKMLADPVIMTVFMVVIVLVCFTICV
                     QGLVSGVERITKIMMLLLFLLMVVLAVHSVLLEGGAPGLEFYLKPDFGKMKEAGLGEA
                     IFAAMGQSFFTLSIGIGALAIFGSYIGKEKRLTGEAISITLLDTSVAFMSGLIIFPAC
                     FAYGIEPTSGPSLIFITLPNVFNHMAGGQLWGALFFVFMTFAALSTVIAVFQNIISFA
                     TDLTGCSVKKAVFWNVIVIILLSMPCVLGFNVLSGIQPLGPGTTIQDLEDFIVSNNLL
                     PIGSVLYLLFCTSRYGWGFKNFVNEANEGEGIAFPTWARVYVSYILPLIVLFILIEGY
                     IG"
     gene            complement(5353..>6804)
                     /locus_tag="ACZPCG_10790"
     CDS             complement(5353..>6804)
                     /locus_tag="ACZPCG_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR1285578.1"
                     /translation="FKGDKEPDIDLNFSGEYQSKAHDYTEVIFGKGQTFRAGTIGTLA
                     DKTAFGYVKKYYEERGTRKRNCEINRIVQGCVGVRRTTGQHPGGIIVLPLGEEIYSFT
                     PVQHPANDMTTSTITTHFDYHSIDHNLLKLDILGHDDPTMIRMLQDLTGLDPVKDIPL
                     DSPEVMSLFQSTEALGITPDDIGGCPLGALGVPEFGTDFAMQMLLDTKPKYFSDLVRI
                     AGLAHGTDVWLGNAQTLIQEGKATIQTAICTRDDIMVYLIARGMEQGLSFTIMESVRK
                     GKGLKPEWEEEMKAHGVPDWYIWSCKKIKYMFPKAHAAAYVMMAWRIAYCKIFYPLAY
                     YAAFFSIRANAFSYEIMCQGRDKLEYYLADYKKRADTLSKKEQDTLRDMRIVQEMYAR
                     GFDFMPLDIYRAKARDFQIIDGKLMPSLSSIDGLGEKAADAVVDAVKDGPFLSVEDFS
                     SRTRVNGTICALMSDLGLLKDLPQSNQLSLFDL"
BASE COUNT         1907 a         1741 c         1287 g         1869 t
ORIGIN      
        1 tgccatacca ctaactgcct tacccacatt aatctccgca cctttaataa caacccgccc
       61 ggcataaatg agcattacag aaagaccgca gagaaacagc acctttaact gatttaatgt
      121 attttcaact tttgctgaca taaacatatc ctcccttatg catctttgcg tcttaacagt
      181 ttgctgtata ctcgatatag cctttccgct actggaattg cgataaaaca ctccatataa
      241 agaccatcaa tattactgca gatattagac atggaagcat aggccataat ttcctctgcc
      301 atctccggga cagttgcagc aaggcttgca gatgatgcag tcatcataga accagaaccc
      361 atgccacagg ccattgccag cgaatatgga tgaagacctg tattaactgc cagagatgca
      421 agaactccaa aaaatactgc gccaaacaga tttcctacca tataggttcc catacagcca
      481 agaaattccg gtgaattggc tccataacgt tcgttaatca gtcccagaga cggctcgcgg
      541 cagatactca ccgttgcgcc aaaggcttcg cgcttcattc caagaagcat ggcaatcgga
      601 agacccacca gcattgttcc cagatttcca aactcctgca gaatcagtgc tggtccagcc
      661 tgaagaattt tcaaaatatt aggtccggaa ctggttccta attttacgcc cagaatcaaa
      721 agcataaaag aaagagctgc ctcactcaat tcaacttcct tttcactgca gatactcttt
      781 aatggtgaaa atattttccc caaagcatcc ggtgttacca gtactgcaat aagcatggca
      841 aataccatgg cagtaatgtt taccttccca acacttccaa gaggaattgt ttttgtgcca
      901 ataatctccg aaataatacc ggcagctgct acaagaacaa aaagtttaat gaatacaggc
      961 caacgaccat ttttactgct taaaatccgg tctacggtta cctctgtttt ctcttttgac
     1021 attcttgaaa acctcctttg tttttgctcc cattatcccc ttactgcttt ttcaacatat
     1081 aaatatcact tctcctgttt tttaacaaag ggagctcctg tctgactttt tttactttgt
     1141 caagatccag agtctgaatc atgaagccct ccctctcttc catttgtccc attatgtcac
     1201 cccagggcga aactgctatt gtatgccccc aggatgtata acctgcatca gcatcccttg
     1261 caggggctgt tccaagggta taaacctgat tgtccaacgc tctggtccgg aaaaggattt
     1321 cccaatgagc cggtcccgtt gtcatattaa aggcggcagg taccagaatc acccgagcgc
     1381 cctcaagcgc catcagacgc gccagttccg gaaaacgaaa atcaaaacaa atacagattc
     1441 ccatttttcc aaattcagta tcaaacactg tcacctgatc accaggcgtc agcgtatcgg
     1501 attctttaaa ggactgtccg ccttcgacct ctatatcgaa cagatgcatt ttccggtgct
     1561 ttgcgatctg ttttccgttg cggtcaaata cataagctgt attaaagata tgcccctgtt
     1621 cgtctgtctc cggaacagat cccgctgaaa ggtaaattcc atattttgct gccagggagg
     1681 aaagagcctg ccaggtttct tctccttcct tttcggcata gacagggaaa ttgctgctct
     1741 gataggggca gttaaacatc tcaggcagcg ttaccagatc cggcttctct tttgccagtt
     1801 cttccagatt tacgcataga gcacggatat tttctgcttt atccgaatat acatgtgact
     1861 gcagcattgc ggcttttaat tttgtcatat gcattcctcc aaaataatct ataatagccg
     1921 atttacatta caaaagtttt cccctgcatt ctacagatat tttttcaatc acctgtccct
     1981 tcgatactag atatgcatat tcatgaagat tcactaccgg acagatatga ttcgggataa
     2041 tttccacctt atctcccact tttacatgat cacggaattg ttgattataa ataatcgcat
     2101 gttcatcgta cacatcgaaa atctcaacat ctggccatcc cttaataagc ccaagaccct
     2161 gtgtggcaca aaaacctttg cttctggtct gtgctgtaat tccctttgca ccaacatctg
     2221 taatcacccg ttcagcagtc ggacggctga taatggttgt aagaactgtc gcagcgtttc
     2281 tgtccagaga gccataggca tttgcctgag aggcatccat gaaaatgtaa gttcccggac
     2341 gtatctccgt aactccctcc agaatcggaa aatcatgcat catcgatgga gtagaaccga
     2401 tactgaccgt ttcaagctcc atcccctcct cttttgccaa acgagcaaat tccagtgtcc
     2461 gtttctgagc cgtcagatga atattccgac attctgaaat atctgccgct ccatatgaat
     2521 tgccatcatg agaaaaaatg cctttcagat aaatattttt gcatgttttc agataatgaa
     2581 gcagttttat ataatcatcc tgttcgatta cgccggaccg ctcctcgcct acttcaattt
     2641 ctataagcac ctgcgccttc ttatctgcac cttcaaatgc ggcttcaatc attttcgcct
     2701 gatcaatcga atctaggcca aaggaaatat caattgattc tgctagctta cgaattcttg
     2761 caagctttac ggctccaaca atttcatttg caataaaaat atttttaagc cctgcagcag
     2821 ccattacctc tgcttcccca accttagcta cggtgattcc ttttgctcca aggtcctcct
     2881 gaatcagcgc aagcttcggc attttatgag ttttagtatg cggccgcagc gccacctttt
     2941 ttctatctgc ataatcctgc ataaatctga gattatccat cataatctca cggtcaatta
     3001 tcagagcagg cgtatcaagt tccatatagt tcataaattt ttactccttt ccgcaggcag
     3061 caaccgcttc tatttccaca agacaacctc catacagatg attcgtcgga acaacaaccc
     3121 tggcaggttt atgcgctcca aaaaattctg agtatacctg attaaactct ccccagtaag
     3181 caacatctgg aagatatacc ctgcattgga gaacatcttc tttatttaag cctgcttctt
     3241 ttaaaactgt atgcagattc ttcaatgcct gggctgcctc tgcttttact cctccctcag
     3301 gaacttttcc tatttccgga tcaatcgaca attgaccgga tatgtacaaa acaccattgt
     3361 attttatgcc cggtacataa tgtcctccgt ctctgacaga atattttgtt tcgataactt
     3421 ctggtttgtt cattgtactg cccccttatg gattgataat catattatag aatattttat
     3481 atataatgtc aataattatt tctatatagt tctgtatttg atagatattt ttactgcttt
     3541 ttatattaaa aactcgattt aatttgcaaa aatatacaat tttatttatc cgcaaaaatc
     3601 gttccttata taaatgtata attttttata taagcgagtg agacaggact cttagacatc
     3661 aaatgagcca gagtatagga aactctggct cataattgtt tctgcatgat ttttacactt
     3721 tcctgttatc caaaaaagct cccacataac gtgtatttaa atagctgcga aaaagttcaa
     3781 acccattttt accccctgtt tacaattttc aaaaaacctg cccaaataga tgagcaggtt
     3841 ctttgagaac atctgtaaat tatccaatat aaccttcaat caaaataaac agcacaatca
     3901 gaggaaggat ataggacaca tagactctgg cccaggtcgg gaaagcaatc ccctctcctt
     3961 catttgcctc atttacaaag tttttaaagc cccatccata ccggctggta cagaacagca
     4021 gatacagcac acttccgatg gggagaaggt tattgctgac aataaagtct tccagatcct
     4081 gaatggtggt tcccggtccc agaggctgaa taccgctcag gacgttaaag cccagaacac
     4141 acggcatgga cagcaggatg attacgatca cattccagaa cacagccttt ttaacggaac
     4201 agccggtcag gtctgtggca aaggaaatga tattctggaa cacggcgatt accgtagaca
     4261 gggccgcaaa ggtcataaat acaaagaaca gagcgcccca cagctgtccc cctgccatat
     4321 ggttaaatac atttggaagg gtaatgaaga taagggacgg accggatgta ggctcgatcc
     4381 cataagcaaa gcaggctggg aaaatgataa gaccggacat aaaagcaact gaggtatcta
     4441 acagagtaat gctgatggcc tctccggtaa gtctcttttc cttgccgata tagcttccga
     4501 aaatcgcaag agcgccgatt ccgatactta aggtaaagaa ggactggccc atagctgcaa
     4561 agatcgcttc tcccagtccg gcctccttca tctttccgaa atccggcttt aaataaaact
     4621 caagacccgg agcaccgccc tctaacagta ccgaatgaac ggccaatacc accattaaaa
     4681 ggaacaaaag gagcatcatg atctttgtga tacgctctac gccggataca agtccctgca
     4741 cgcagatggt aaagcatacc agaacaatga caaccataaa tactgtcatg atcaccggat
     4801 cagccagcat tttgctgaat tcctctgccg taccggcaga atcaagtcca ttaaagtcgc
     4861 cccggagcat tttaaaaaag tacagaagca tccagccggc tactgtcgtg taaaacatca
     4921 tcagcatata gtttccggcc attgctatat attttgtcag gtgccatttg cttcccttgg
     4981 gttccagcag atcaaaggac agagccgcac tctttttgct ggcccggccc acggcaaact
     5041 ccatcactac aataggaagt cccaggacca gaagaaaagc aaggtagatc agcacaaagg
     5101 cggctccacc gtattttccc acaatatagg gaaaacgcca tacattgccc agaccgatgg
     5161 cgcatcctgc tgaaatcagg ataaagccca gcctggaaga aaacttttca cgttccattt
     5221 ctcatatctc ccgtaatttt tttgtattcc ggcccttatg cattcccccg caaagccgga
     5281 aaagagcttt ttataaaaaa agctcttccg tatcataaca cagattaaat aaaatggcaa
     5341 cggctgtctt ttttacagat caaacagaga aagctgattg gactggggca gatcctttaa
     5401 aagtcccaga tccgacatca gggcacagat cgttccgttt acgcgggtac ggcttgaaaa
     5461 atcctccaca gacaggaatg ggccgtcctt taccgcatca acgaccgcat ccgctgcctt
     5521 ttctcccaga ccgtcaatac tgcttaaaga aggcatcagc ttaccatcaa tgatctggaa
     5581 atcccttgcc tttgccctgt agatatcaag aggcataaaa tcaaagcctc tggcgtacat
     5641 ttcctgaaca attctcatat ctcttaatgt atcctgttcc ttttttgaca gggtatctgc
     5701 ccgtttttta taatctgcca gatagtactc cagcttgtcc cggccctggc acatgatttc
     5761 ataggaaaat gcattagcgc ggatactgaa aaaggctgca tagtaggcta acggataaaa
     5821 aatcttgcag taggcgatcc gccacgccat cataacgtaa gctgccgcat gggccttggg
     5881 gaacatgtat ttaatctttt tacaggacca gatataccag tcaggcaccc cgtgggcttt
     5941 catttcctct tcccattcag gttttaatcc ctttcccttc cgcacactct ccatgatcgt
     6001 aaaggaaagc ccctgctcca tccctctggc gatcagataa accataatat catctcgggt
     6061 acagatggcg gtctgaatgg ttgccttccc ctcctggatt aacgtctggg catttcccag
     6121 ccatacatcc gtaccgtgag ccagtcctgc gatccggacc aggtcggaaa agtacttcgg
     6181 ctttgtatca aggagcatct gcatggcaaa atccgtacca aactcaggta cgcccaatgc
     6241 tcccagcgga cagccgccga tatcgtccgg agtgatcccc agcgcctcag tgctctgaaa
     6301 caggctcata acctccggac tgtccagagg gatatcctta accgggtcaa gaccagtcag
     6361 atcctggagc attcgtatca tggtgggatc atcgtgtccc agaatatcca gctttaacag
     6421 gttgtggtca atggaatgat agtcaaagtg ggtcgttatg gtggaggtcg tcatatcgtt
     6481 ggccgggtgc tgcaccggag taaaggaata aatctcctct cccagcggaa gcacgataat
     6541 gcctccggga tgctggcctg tggtacggcg cacgcctacg cagccctgga cgatccggtt
     6601 gatctcacag ttgcgctttc tggttcccct ttcctcataa tattttttta catagccaaa
     6661 ggccgtctta tctgccagag taccgatggt acctgcccgg aaggtctgac cttttccaaa
     6721 aataacctct gtgtaatcat gagccttact ctggtactca ccggaaaagt ttaagtcaat
     6781 atcaggctcc ttatcgccct taaa
//
LOCUS       DBMDUH010000142         6727 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_4763_length_6727_cov_5.561274, whole genome shotgun sequence.
ACCESSION   DBMDUH010000142 DBMDUH010000000
VERSION     DBMDUH010000142.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 6727)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6727)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6727
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4763_length_6727_cov_5.561274"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1092)
                     /gene="ffh"
                     /locus_tag="ACZPCG_10795"
     CDS             complement(<1..1092)
                     /gene="ffh"
                     /locus_tag="ACZPCG_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HIR1285579.1"
                     /translation="MAFESLSDKLQNIFKNLRGKGRLSEADVKAALKEVKMALLEADV
                     SFKVVKQFISSIQERAVGEEVFGSLTPGQTVIKIVTEELIKLMGSETTEIALKPASEI
                     TVIMMAGLQGAGKTTTTAKIAAKLKAKGRKPLLAACDVYRPAAIKQLQVNGEKVGIPV
                     FTMGDKISPVDIAKGAIEHASKNGFNVVILDTAGRLHIDESMMDELVGIREAVEVYQT
                     ILVVDAMTGQDAVNVAGTFNDKVGIDGVILTKLDGDTRGGAALSIRSVTGKPILYIGM
                     GEKLSDLEQFYPDRMASRILGMGDIQSLIEKAAAEVDEEQAKELSQKLRKAEFDYNDF
                     LTQMQQIKKMGGMGSILSMMPGVGSQLSGI"
     gene            complement(1126..1470)
                     /gene="ylxM"
                     /locus_tag="ACZPCG_10800"
     CDS             complement(1126..1470)
                     /gene="ylxM"
                     /locus_tag="ACZPCG_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048500 - signal recognition particle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxM family DNA-binding protein"
                     /protein_id="HIR1285580.1"
                     /translation="MEKIVEQGLLYDFYGELLTEHQRRIYEDMVFNDLSPSEIAKEQG
                     ISRQSVHDLKKRCDRILEEYEEKLHLVAKFMKIRDMVRELDGLAETCTKTRDIQLMDE
                     IRSLSGKIMAAL"
     gene            complement(1512..2984)
                     /locus_tag="ACZPCG_10805"
     CDS             complement(1512..2984)
                     /locus_tag="ACZPCG_10805"
                     /inference="COORDINATES: protein motif:HMM:NF018837.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HIR1285581.1"
                     /translation="MGLFSFGRKKLEDGFEFVIDGIYALKGNSAVAAGKLTRGRLIPG
                     TEAICLNELGEPVFKCRISGIEQGTKILRAASYDMKGTYGAHYGFKLDGVTKEQIPAE
                     GAVLVSVTEELEEALKDAAPVSSVKAKASVENTVVVRDAQAQNALLMAARAQEKAGRP
                     AGPLGAEREEQLSAVLEETLSEAVLVPLSIQESIFLLCRLQQMNHQEPVSGYEDKIQM
                     LYDTVLEKLKAAPSLFILLDEGSSMPFMIGDTVDVYSAKELALKALHFYAEQYHRHLV
                     LREIPREHTGLPGRLSLFAWLYYLGMEKLLIDNGSYQLFVNRSDLLEHPEEEAGQPLE
                     VPVVNPSLRCAMAEYLQEIRWNVSYSEREANVEAKKEHLKKELLQAKLLVPVKFEKTE
                     GLNKGQNTIDAEKQDNLFFPRIENNEHKQFLPMFTDWLEFQKAYKREEWGCMVFSLAD
                     GLRAAAGDPAVINPLTENLILDRALVKEIAEMYRSSSKNA"
     gene            complement(3088..4707)
                     /locus_tag="ACZPCG_10810"
     CDS             complement(3088..4707)
                     /locus_tag="ACZPCG_10810"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003503389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HIR1285582.1"
                     /translation="MKMTAIQPESWKEDYRQFEQATEQFYSGEMDAKTYKGISGGFGS
                     YAQKGGKASMLRLRMPGGVMTKEKLAFVVQSIKEYEVKQVHLTTCQTLQLHDLSKDQV
                     CELAVKALSCGIVTRGGGGDFPRNVMVSPLAGVEEGEYFDVLPYALKAADYLMSFIKG
                     KKLPRKLKVGFSNTPKNVTHATYRDLGFAAREDGTFDVYSAGGLGNKPAFGVRVAEGV
                     APDQILYYIRAMHELFCAYGNYENRAKARSRFMQEALGGAEAYKEAFLKKLDEVYAEE
                     GDLTLEMGETSLSEEAVQDQSTAFAASREILFASISDPYMRKRVIPQKQNGLYSVACH
                     PLGGSPEPSLFADLYEVIESIPGAELRLSPEEMFYVINCRAEDVKKVLEVTSSEKAVC
                     SCSRFEESVACIGASICQQGVRDSQALLHRLIEMEREEGFEDGILPQIHISGCPSSCG
                     THQTGVIGFRGGVKSVNKEVKPAFNLFVKGCCFQGKERMGEELGAILEENIPAFLKAL
                     GHAVQESGLDFEGWYAKDAEGIRKIAENFLA"
     gene            complement(4652..>6727)
                     /locus_tag="ACZPCG_10815"
     CDS             complement(4652..>6727)
                     /locus_tag="ACZPCG_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HIR1285583.1"
                     /translation="SKAYPKYLEEAREYLKRRLFEETGKSADVFVLADLLEVKHPEWA
                     NAIEGYLGNNKLSLVVSPRYARAALKIYSELDPKKYYSVAVLDTEKAASSQAAVLKNS
                     LAEEIGVKEAFLAPYMELLLGKVIKCRSIEELSDCKIGITPECLLYHNLRLQHINPEH
                     YTRMAYIGKDSLRKRIRLVQEELEETKKARLPLEAVLADCRRISGLEALKGPAEEYMQ
                     WLEEIKAWKKKESEKKQLSRRLEELKAQNIGQLEEERSAVLEQCEGVKRERDRLRELI
                     LGRKNELERFSHSITELEETAASQNGQLEENAEHDRELSAYLAEREAIRYDRERETYE
                     NKCRILTEQSNKARETLMNLRTEYIRSYPGRNVPVSARDNEAYDSLLDRLSCDDLERF
                     KEQAARQARSAVEHFKDDFMYKIRSAIKEAMLRTEELNRIISRLDFGKDKYRFVISRS
                     KGADGRYYDMFMDESLEVDPSRLDHSFENQLNLFTMEHENHYGDRINELISIFIPPEN
                     AAPEQLEEARRNMEKYSDYRTYLSFDMQQLVQNGDEVIKIRLSKMLRKNSGGEGQNPL
                     YVALLASFAQAYHIGQPAGLRKSPSIRLVILDEAFSKMDAEKVASCIELIRGLGFQAI
                     ISATNDKIQNYLENVDRTFVFANPNKKAISIQEFERGRLKEFTQERKVIYEDDSNSAG
                     ELERGLQTV"
BASE COUNT         1598 a         1894 c         1348 g         1887 t
ORIGIN      
        1 aatgccggaa agctggcttc caacaccggg catcatggaa agaatacttc ccataccgcc
       61 catcttttta atctgctgca tctgggttaa gaagtcattg tagtcaaact ctgccttgcg
      121 cagcttctgt gaaagctcct ttgcctgctc ctcgtctacc tcagccgccg ccttctcgat
      181 caaggactgg atatctccca ttcccaagat tcttgaagcc atacgatccg gataaaactg
      241 ttccaaatca gaaagctttt ctcccatacc aatgtagagg atcggctttc ctgtcacgga
      301 acggatggaa agagccgcgc cgcctctggt atcgccgtcc aacttggtaa ggattacgcc
      361 gtcaatgccg accttgtcat taaaggtacc agctacattt accgcatcct gaccggtcat
      421 ggcgtccact accagaatgg tctgatatac ttctactgct tcccggatgc ctaccagctc
      481 gtccatcata ctttcatcaa tatgaagacg gcccgcagta tccagaatca ctacgttaaa
      541 gccatttttt gaggcgtgtt cgatggcgcc ctttgcaata tccacaggac tgatcttatc
      601 gcccatggta aatactggga ttcccacctt ttcgccattg acctgcagct gcttgatggc
      661 tgcgggacgg tatacatcgc aggccgcaag aaggggcttt ctgccctttg cctttagctt
      721 ggctgcaatc tttgccgtcg tggtcgtctt acctgcaccc tggagacctg ccatcataat
      781 gaccgtaatc tcacttgcgg gttttaaggc aatctctgtt gtctcagaac ccataagctt
      841 tatcagctct tcggttacaa tcttgattac cgtctgtccc ggtgtcaggc ttccaaatac
      901 ctcttcgccg accgcccgtt cctgaatgga acttataaac tgctttacta ccttgaagct
      961 tacgtcggct tccaaaagag ccatctttac ttcctttaag gcagctttta catcggcctc
     1021 agacaggcgg cctttgccgc gcaggttttt gaaaatattc tgcaatttat cggataagct
     1081 ttcaaaagcc attgataacc tcctgaaggt cttttgctgt agggatcaca gcgctgccat
     1141 gatctttccg gacaggcttc ggatctcatc catcagctgt atatcccggg ttttggtaca
     1201 ggtctctgcc agaccgtcca gctccctgac catatcacgg attttcatga attttgccac
     1261 aagatgcagc ttttcttcat attcctccag tatacggtca cagcgctttt tcagatcatg
     1321 gacgctttga cggctgattc cctgctcctt tgcaatctcg ctgggagaaa ggtcattaaa
     1381 caccatgtcc tcatagatgc ggcgctggtg ctccgtcagc agttctccat aaaaatcgta
     1441 cagcagaccc tgttcaacga tcttttccat ttcaaccacc tgtgttatca tacacgatag
     1501 ctgcaatctt gtcaagcatt tttacttgac gaacggtaca tttctgcaat ttcttttaca
     1561 agagctctgt ccaggatcag attttccgtc aaaggattga taacagcagg atctcccgct
     1621 gctgcccgaa gtccgtctgc caggctgaac accatgcagc cccactcttc cctcttatat
     1681 gccttttgaa attccagcca gtctgtaaac ataggcagga actgcttatg ctcattgttt
     1741 tcaatccgcg ggaagaagag gttgtcctgt ttctctgcat caatggtatt ctgtccctta
     1801 tttaacccct ctgttttctc aaattttacc ggcaccagaa gctttgcctg caaaagttct
     1861 ttctttaagt gttccttttt cgcttccaca ttggcctcgc gctctgagta agacacattc
     1921 cagcggattt cctgcagata ctctgccatg gcacagcgca aagaaggatt aaccacaggc
     1981 acttccaatg gctgtcctgc ttcttcttcc ggatgttcaa ggagatctga ccgatttaca
     2041 aaaagctgat aggagccatt gtcaataaga agtttctcca tgccaagata gtacagccat
     2101 gcaaaaaggc ttaatcggcc cggaaggccg gtatgctccc ttggaatctc ccgaagcacc
     2161 agatgacggt gatactgctc tgcataaaaa tgaagggctt taagagccag ctcctttgca
     2221 gaatatacat caaccgtatc tccgatcata aaaggcatac tggagccttc atccaaaaga
     2281 ataaacagag aaggcgctgc tttcagcttt tcaagaaccg tatcataaag catctgaatc
     2341 ttatcttcat atccgcttac aggctcctga tgattcatct gctgcagacg gcaaagcagg
     2401 aaaatggatt cctgaataga aagcggaacc agaactgctt ctgaaagtgt ttcttccaaa
     2461 acagcgctta actgctcctc ccgctctgct cccaggggac cggcgggccg gccggctttt
     2521 tcctgtgctc tggctgccat aagaagtgca ttctgagcct gtgcatcccg gactactacc
     2581 gtattttcca cagaagcttt ggccttaaca gaagaaacag gtgctgcatc ctttaatgcc
     2641 tcctccagct cctctgttac agataccagc acagctccct ctgccgggat ctgctccttt
     2701 gtcacaccat ccagtttaaa gccgtaatga gccccatagg ttcccttcat atcataagac
     2761 gcagcccgca aaatcttggt tccctgctca ataccggaaa tccggcactt aaacaccggc
     2821 tcacccaatt catttaagca gatagcttct gtccctggga tcagacgccc ccttgtaagc
     2881 tttcccgcag ccacggcgct gtttcccttc agagcgtaaa tgccgtcaat gacaaattca
     2941 aatccgtctt caagtttttt tcttccaaat gaaaataatc ccattgtttc ctcctggttg
     3001 ttccggaata tccatcattc cggtatgtct ggtagagatt atacaacact cccgcttaaa
     3061 aggaaagacc ttttaaaaag tgccctttta tgccaggaag ttctctgcaa tcttgcggat
     3121 tccttccgca tcctttgcat accagccttc aaaatcaagg ccgctttcct gtaccgcatg
     3181 gcctaaggcc tttaaaaatg ccggaatgtt ctcttcaagt atcgctccca gctcttctcc
     3241 catccgttct tttccctgga agcagcatcc ctttacgaaa agattaaaag caggtttgac
     3301 ttctttattt actgatttta caccacctct gaagccgatt actcctgtct ggtgagtgcc
     3361 gcaggaagat ggacagccag aaatatggat ctgaggcagg ataccgtctt caaagccctc
     3421 ttcacgctcc atttcgatca gacggtgaag cagagcctgg gaatctctta caccctgctg
     3481 gcagattgat gcgccgatac atgctaccga ttcctcaaaa cggctgcagg agcagaccgc
     3541 cttttcagaa gaggtaacct ctaacacctt ttttacatcc tctgcccggc agttgatcac
     3601 ataaaacatt tcttccggag acaaacgaag ttccgcgccg ggaatagatt ctatcacctc
     3661 ataaagatct gcaaaaagag aaggctccgg gcttcctcca agcggatggc aggctacgct
     3721 gtaaagcccg ttctgtttct gagggattac ccgctttctc atataaggat ctgaaataga
     3781 ggcaaataaa atctccctgg aagcagcaaa agctgtactc tgatcctgta ccgcctcttc
     3841 ggataaggaa gtctctccca tttccagagt aagatctcct tcctccgcat aaacctcgtc
     3901 cagcttcttt aaaaatgctt ccttatacgc ctcagcacct cccagagctt cctgcataaa
     3961 acggcttctg gctttggccc tgttttcata attgccatac gcacagaaca gctcatgcat
     4021 ggctcggata tagtaaagga tctgatccgg agccactccc tctgccaccc ttacgccaaa
     4081 agcaggctta tttcccagac cacctgcgct gtatacgtca aaggtgccat cctctctggc
     4141 cgcaaagcca agatccctgt acgtcgcatg cgtgacattc tttggcgtat tagaaaatcc
     4201 caccttcagc tttcggggca gttttttccc cttgataaag ctcataagat aatcagccgc
     4261 ttttaaagca taaggaagaa cgtcaaaata ttctccctcc tccacaccgg ccagaggaga
     4321 aaccattaca tttctcggaa aatctccacc accgcctctt gtgacaatcc cgcaggaaag
     4381 ggcttttaca gccagctcac aaacctgatc ctttgacaga tcgtgaagct gcagtgtctg
     4441 acaggttgtt aaatgaacct gtttaacctc atattcctta atactctgta ccacaaaggc
     4501 cagtttttcc tttgtcatca caccgcccgg catacgcaga cgaagcatgc tggcctttcc
     4561 gcccttttga gcatagcttc cgaaaccgcc cgaaatgccc ttataagttt tggcatccat
     4621 ctctccgctg taaaactgct ccgtggcctg ttcaaactgt ctgtagtcct ctttccagct
     4681 ctccggctga attgctgtca tcttcataga taaccttcct ttcctgtgta aactctttta
     4741 agcgcccccg ttcaaattcc tgaatggaaa tcgctttttt gtttggattt gcaaatacaa
     4801 aggttcgatc cacattttcc agatagttct ggatcttatc gttggtagcg ctgataatcg
     4861 cctggaagcc cagtccccgg atcagttcaa tgcagcttgc caccttttcc gcatccatct
     4921 tggaaaaggc ctcgtctaag atcaccaggc ggatcgacgg gctttttcga agtcctgcag
     4981 gctgtccgat atggtaagcc tgtgcaaaac ttgcaagaag cgctacgtac aggggatttt
     5041 gcccctctcc gccggaattt ttccgaagca ttttgcttaa acggatcttt ataacctcgt
     5101 ctccgttctg aaccagctgc tgcatatcaa aagaaagata ggtccggtaa tcggaatatt
     5161 tttccatatt ccgtctggcc tcctcaagct gctccggcgc agcgttttcc ggcgggataa
     5221 aaatgctgat cagctcgtta atcctgtctc cgtaatgatt ttcatgctcc atggtaaaaa
     5281 ggttcagctg attttcaaag ctgtgatcca gccgggaggg atctacctct aaagattcat
     5341 ccataaacat atcataatac cgtccgtctg ctcccttaga ccgcgaaatc acaaacctgt
     5401 atttatcctt tccaaaatcc agacggctga tgatccggtt cagttcttca gtccgcagca
     5461 tggcctcctt aatagcgctg cggatcttgt acataaaatc atccttaaaa tgctccactg
     5521 ccgatcgggc ctgcctggca gcctgctcct taaaccgttc cagatcatca caggaaaggc
     5581 ggtcaagaag actgtcatac gcctcgttat ctctggcact taccggcaca ttgcggcccg
     5641 gatagctgcg gatatactcg gtacgaagat tcataagcgt ttctctggcc ttattactct
     5701 gttctgtcag gatcctgcat ttattttcat aggtttcccg ctctctgtca tagcgtatgg
     5761 cctccctttc tgccagataa gccgaaagtt ccctgtcgtg ctccgcattt tcctcaagct
     5821 gtccattctg ggatgctgct gtttcttcca gctctgtaat agaatgggaa aaacgctcca
     5881 attcattttt gcggccaagg atcagctccc tcaggcggtc ccgttcccgt ttcactcctt
     5941 cacactgctc aagaacagcc gaccgttcct cttccagctg tccgatattt tgggctttca
     6001 gctcctctaa ccgtctggat aactgcttct tttcggattc ctttttcttc caggccttaa
     6061 tttcctccag ccactgcata tattcctctg ccggcccttt taacgcctca agccctgata
     6121 tccgcctgca gtccgcaaga accgcttcca ggggcagtcg ggctttttta gtctcctcca
     6181 gttcttcctg aaccagccgg atcctctttc gcaggctgtc ctttccaatg tacgccatac
     6241 gggtataatg ctccggattg atatgctgaa gacggagatt atgatataaa aggcattctg
     6301 gagtaatacc gattttgcag tctgaaagct cctcaatgga gcggcatttg atcacttttc
     6361 caagcaaaag ctccatatat ggagcaagaa acgcttcttt aaccccgatt tcctctgcca
     6421 gagaattttt aagtaccgca gcctgagaag aagcagcttt ctctgtatcg agaaccgcca
     6481 cactgtaata ttttttggga tccagctcac tgtaaatctt taaagcggcc ctcgcatacc
     6541 tgggggaaac caccaggctt aatttgttgt ttcccagata cccctctatg gcattggccc
     6601 attccggatg ctttacttcc agaagatcag ccagcacgaa tacatctgcg ctttttcctg
     6661 tttcctcaaa caggcgccgt tttaaatact ctcttgcctc ctccagatat ttcggatagg
     6721 ccttgct
//
LOCUS       DBMDUH010000143         6689 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_4795_length_6689_cov_6.836369, whole genome shotgun sequence.
ACCESSION   DBMDUH010000143 DBMDUH010000000
VERSION     DBMDUH010000143.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 6689)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6689)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6689
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4795_length_6689_cov_6.836369"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="ACZPCG_10820"
     CDS             complement(<1..942)
                     /locus_tag="ACZPCG_10820"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HIR1285584.1"
                     /translation="MAKEMIAMLLAGGQGSRLYALTQKLAKPAVPFGGKYRIIDFPLS
                     NCVNSGIDTVGILTQYQPLVLNEYIGNGQPWDLDRLHGGVHVLPPYQKASGSDWYKGT
                     ANAIYQNISFIERYDPQYVIILSGDQICKQDYSDFLRFHKEKNAEFSVAVMEVPWEDA
                     SRFGLMVADENDRITEFQEKPKNPKSNLASMGIYIFNWDILRKYLIEDEEDPDSENDF
                     GNNIIPNLLKDGRRMYAYHFDGYWKDVGTIPSLWEANMEVLDPEHSGINLFDENWKIY
                     SRNSGMTGHKISDGGVVENSMITDGCRIAGTVRHSVLF"
     gene            complement(1158..1748)
                     /locus_tag="ACZPCG_10825"
     CDS             complement(1158..1748)
                     /locus_tag="ACZPCG_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HIR1285585.1"
                     /translation="MSLTPPITIRPVSTADSQALLDIYRPYVEHTAITFEYEVPTAEE
                     FTRRIRHTLKRYPYLAAEKDGQLLGYAYAGPFHERAAYDWCAEVSIYIRTDARGTGIG
                     KKLYEALEHALTAQNILNLYACIAWPDKEDEYLTRNSADFHRHLGYELAGMFKNCGYK
                     FDRWYSMIWMEKQLGPHSGHAEPIIPFQELLHRMPV"
     regulatory      1891..2060
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2176..3657
                     /locus_tag="ACZPCG_10830"
     CDS             2176..3657
                     /locus_tag="ACZPCG_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HIR1285586.1"
                     /translation="MIEFLNWLDGVLWGIPLIVLMISTGIYFTVRSGFFQFRRFGWIL
                     KNTGGTILNKKSQKQEDNAKGMLSSFEAISTAIGGTVGFGNIAGVATAVAAGGPGAVL
                     WMWLSACLGMILKQVEVTLGCYYRHTNEKGEYYGGPTYYMERGLGEERRWGKLWLIPA
                     VIFGAGIFSTFFVTSSTLTASQVVAGAFKMDTVNIGGFQIEGVILVGAALCVLTYIVT
                     DGGTKKIASLFSKLVPLMSVFYILMGIGMILANLSRVPSVFAAIVTNAFTGTAAIGGF
                     AGCAVSEMIRVGMARSVYSNEAGWGTSPMIHSSARTPHPVAQGLWGSFEVFFDTFVVC
                     SITSVSVLLSGNWTNGTDGGTLALSAFSQSFGTAGSLLLALIMVIFTVTTSGGWFTYY
                     LSILEHLCKKEGPMKKVMLKLFYAVRALPGLIWTIYLVKTNNQGFIWTVVDITSAIPT
                     FVNVAVILLLSKDYFRLLKDYKARYLGEGTAELDACLFYEEKH"
     gene            3675..5288
                     /locus_tag="ACZPCG_10835"
     CDS             3675..5288
                     /locus_tag="ACZPCG_10835"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002600790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR1285587.1"
                     /translation="MKHIYFNGKVYTGSLPLAEAFAVENGKFIFAGSSKSVLKLAGEG
                     DVCTDLKGRFVCSGFNDSHMHLLGYGNSLCSARLAARTESLADMLQCLKEFYRENPCA
                     EGEWLMGRGWNQDYFTDADRMPDRHDLDQVSEDVPVCAVRACGHCLVVNTKALQLLGI
                     TKDTPQPEGGCIGMDEEGPDGRFFDNAMDLVYHAIPVPDKEGIKNRIRLACRALNSYG
                     VTSSQTDDYCMYRDVPWQTVNEAYRELEAEGELTVRVYEQCNFTELSELKAFVEAGNV
                     TGSGTELFKVGPLKMLGDGALGARTAYLSRPYADDPSTCGIPVFSQEVFDEMIGYANA
                     HGMQAAVHAIGDACLDRVLDAYEKALKEHPREDHRHGIVHCQITRPDQLERIARLKLH
                     VYAQSIFLDYDNHIVKDRVGEELASTSYSWKTLMKQGVSVSNGTDCPVELPDALASME
                     CAVTRTTIKDHVGPYLPDEAFTVQEALDSYTIRGAEGSFEEKIKGKICPGMLADFVIL
                     EQSPFETAPEQLHTIPVHAAYLGGRKVFG"
     gene            5297..5932
                     /locus_tag="ACZPCG_10840"
     CDS             5297..5932
                     /locus_tag="ACZPCG_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006444940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HIR1285588.1"
                     /translation="MTKKELALKLIERLKKAYPEASCTLDYNQAWKLLVGVRLAAQCT
                     DERVNIVTEKLYKKFPDVDALAAASPEEIEEIVRPCGLGRSKARDISACMRILKEQYG
                     GQVPETFDDLLKLPGVGRKSANLIMGDVFGKPAIVTDTHCIRLVNRMGLVEGIKDPKK
                     VEMALWKLIPPKEGSDFCHRLVFHGRAVCSARTKPDCEHCCVSDLCARNGV"
     gene            5998..>6689
                     /locus_tag="ACZPCG_10845"
     CDS             5998..>6689
                     /locus_tag="ACZPCG_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR1285589.1"
                     /translation="MLEDEWIYVVEEGDTVDEIAAKTGRPVGELIWANQLEYPYRLAV
                     GQSLYVGAEGSIGAGSPLLYSFGYAYPFIDRDTLDETLPFLTDIYIFSYGFTAEGDLV
                     SPHIEDTPVIQSARTAGVRPVMVLTPLGPDGRFNNRLITELLSNDRAVRRLVWQLMAV
                     IEQKEYEGLDIDFEYVPAGDRERFAEFVRLCAGVFRALGIFVSAALAPKTSDDQPGLL
                     YEGIDYRAIGEAA"
BASE COUNT         1656 a         1455 c         1874 g         1704 t
ORIGIN      
        1 gaagagtacg gagtggcgta cggtgcctgc aatacggcag ccgtcggtga tcatggagtt
       61 ttctacaacg cctccgtctg aaatcttgtg tccggtcata ccgctgttac ggctataaat
      121 cttccagttt tcgtcaaaca ggttgatgcc gctgtgctcc ggatccagaa cctccatatt
      181 ggcctcccag agagaaggaa tcgttcctac atccttccag tagccgtcaa agtggtaggc
      241 gtacatcctg cgtccgtcct ttaagagatt agggatgata ttgttgccga aatcattttc
      301 agaatccgga tcttcctcat cttcaatgag atattttctt aaaatatccc agttaaagat
      361 atagataccc atagatgcca gattggattt aggatttttg ggtttttcct ggaattcggt
      421 gatgcggtcg ttttcatccg ctaccatcag gccaaagcgg gaagcatcct cccaggggac
      481 ttccatgaca gccacgctga attccgcatt cttttcctta tggaagcgga gaaaatcgct
      541 gtaatcctgc ttacagatct gatcgccaga aaggatgatc acatactggg gatcatagcg
      601 ttcaataaag gatatattct gataaattgc attggctgta cccttgtacc agtccgatcc
      661 ggaggctttc tgatagggcg gaagcacatg aactccgccg tgaaggcggt ccagatccca
      721 gggctgtcca tttccgatat attcattcag taccagaggc tgatactggg tcaggatacc
      781 tactgtgtca attcctgaat ttacgcagtt tgacagcgga aagtcgataa tacggtattt
      841 tccgccaaaa ggaactgcag gttttgccag cttttgagtc agggcataaa gccgtgaacc
      901 ctggccgccg gcaagcagca ttgcgatcat ttcttttgcc atttcaaatt ctccccttgt
      961 atagtagtga cgcctcggtc cgcagaacac ataagccact ctgcttaccg ggcagcattc
     1021 ccaacgaaaa acatttcagg aatgttctgt tagaattgta gcacaaatcg gtaaaaacgg
     1081 acggtatttt gtgaaaaaag aacatgaatg caatattcaa aagacaaagt gcaaaatcat
     1141 gcataataga agatgcttta aacaggcatc cggtgaagca gttcctggaa agggataatg
     1201 ggttctgcat ggccggaatg ggggccaagc tgtttttcca tccagatcat actgtaccag
     1261 cggtcgaatt tatagccgca gtttttaaac attcctgcca gttcatagcc cagatggcgg
     1321 tggaaatcag cgctgtttct ggtaagatat tcatcttcct tatccggcca tgcgatgcag
     1381 gcgtaaagat tgaggatatt ctgggcagta agtgcgtgct ccagagcttc atagagtttt
     1441 tttccaatcc ctgtgccgcg ggcatcagtc cgtatataaa tggaaacctc agcgcaccag
     1501 tcataggctg cccgttcatg aaaagggcct gcataagcat agcctaaaag ctgtccgtct
     1561 ttttctgctg ccagataggg gtagcgcttt aaggtgtggc ggatccggcg ggtaaattcc
     1621 tccgcagtgg gaacctcata ttcaaaggta atggcagtat gctccacata gggacggtaa
     1681 atatcaagga gagcctggct gtcagcggtg gaaacggggc ggatggtgat gggtggtgtg
     1741 agtgacataa tgtgtacctc cggataaaac atatgatttt ttagtatagg acgaaaaaaa
     1801 gattgtatca agtgaaattt ggccgaagaa ataaacttgc aaaaacagtt gacagccagc
     1861 gtgaaaccag ctatgatact acaaaaagag tacggcaata gaggtgcgga tactatcagt
     1921 acctgtttgg aggcagcgaa gccagggaag agcagggaaa ggaggagccg ccgaaatgta
     1981 ctgctttcgc aggcagtatg ttggggtgca gtcgaacagg ctgcacactc tcaccaaacg
     2041 gtggaggcgc tattgtttca tggatggatg atttatggag cagtgggatg caaaaaccgc
     2101 tgctcctttt ttgcgggcag agcccaggtg gaatagattg ctgtacataa acaaaagaaa
     2161 agggggatca taaaaatgat cgaatttctc aactggttag acggcgtcct gtgggggatt
     2221 ccgttaatcg tactgatgat ttctaccggt atttatttca cagtccgttc cggattcttt
     2281 cagttcaggc gctttggctg gattttaaag aataccggtg gcacgatttt aaacaaaaaa
     2341 agccagaagc aggaggataa tgcaaaggga atgttaagtt cctttgaagc aatttccaca
     2401 gcaatcggcg gtacggtagg ctttggaaat attgccggtg tggctacggc agttgcggca
     2461 ggaggccctg gcgctgtgct gtggatgtgg ctgtctgcct gtcttggaat gatcttgaag
     2521 caggtagagg taacgctggg ctgctattac cgccatacca atgaaaaggg tgaatattac
     2581 ggcggcccta cctattatat ggagcgggga ctgggagagg agcgccgctg gggaaagctg
     2641 tggctgatac cggcagttat tttcggcgca ggtattttct ctacattctt tgtaacctcc
     2701 tctacgctga ctgcttccca ggtggtagca ggagccttta aaatggatac ggtgaatatc
     2761 ggaggctttc agattgaggg cgttattctg gtgggagcgg ctctttgcgt tctgacctat
     2821 attgtaacgg acggaggcac caaaaagatt gcgtctttgt tcagcaagct ggtcccctta
     2881 atgagcgttt tctacatttt aatgggaatt ggcatgattc tggcaaatct ttccagagtg
     2941 ccctctgtgt ttgctgccat tgtaacaaat gcgttcactg gtacggcagc catcggcggc
     3001 tttgccggct gtgcagtcag tgagatgatc cgggtaggta tggcacgttc cgtatactcc
     3061 aatgaggccg gctggggtac ttctcccatg atccattcct ctgccagaac accccatcct
     3121 gttgcacagg gactttgggg ttcctttgaa gtattttttg atacctttgt agtctgctcc
     3181 atcacatctg tttctgtgct tttaagcgga aactggacca acggcacaga cggcggcact
     3241 ctggcattaa gcgccttttc ccagagtttt ggtactgcag gaagcctgct tttagctttg
     3301 atcatggtta tttttactgt caccacctcc ggcggctggt ttacctacta tctgtcaatc
     3361 ctggagcacc tttgcaaaaa ggaaggcccc atgaaaaaag tgatgctgaa gctgttttac
     3421 gcagtccgtg cgcttccggg ccttatctgg acgatttatc tggtaaagac aaataatcag
     3481 ggctttatct ggaccgtggt agacattact tctgccattc ccacctttgt gaatgtggct
     3541 gtgatcctgc tgctgtcaaa ggactatttc cgtctgttaa aggattataa ggcacggtat
     3601 cttggagagg gaacagcaga gctggatgcc tgcctgttct atgaagaaaa gcactgacga
     3661 aaggagaatg tgccatgaaa catatttatt ttaatggaaa ggtttataca ggcagtcttc
     3721 cgctggctga ggcctttgcg gtagaaaatg gtaaatttat ctttgccggt tccagcaaat
     3781 ccgttctaaa actggccggg gagggagatg tgtgtaccga tcttaagggc cgttttgtat
     3841 gcagcggttt taatgacagc catatgcatc ttttaggata cggaaacagc ctgtgttctg
     3901 cccggctggc tgcccgtaca gagagcctgg cggatatgct tcagtgcctg aaggagtttt
     3961 acagggagaa tccctgcgca gagggcgaat ggctgatggg ccggggatgg aatcaggatt
     4021 attttacaga tgcagaccgg atgccggatc gtcatgatct ggatcaggta tcagaggacg
     4081 tgcccgtctg tgcggttcgg gcctgcggtc actgtctggt cgtaaataca aaggcattac
     4141 agctgcttgg catcacaaag gatacgcccc agccggaggg cggatgtatt ggtatggatg
     4201 aggaggggcc ggacggccgt ttctttgaca atgccatgga tctggtgtac catgcaattc
     4261 ccgttccgga taaggaaggg atcaaaaaca ggatccgtct ggcctgcagg gctttaaatt
     4321 cctacggtgt taccagcagc cagacggatg actactgcat gtaccgggat gtgccgtggc
     4381 agactgtgaa tgaggcctac agggagctgg aggcagaggg agagcttacg gttcgtgtct
     4441 atgaacagtg taacttcaca gagctttcag agcttaaggc ttttgtagag gcgggaaatg
     4501 tcacgggcag cggtacagag ctttttaagg tcgggcctct taagatgctg ggagacggag
     4561 cattgggagc cagaactgcg tatctgagcc gcccttatgc ggatgatccg tccacctgcg
     4621 ggatccctgt attttcccag gaggtttttg acgaaatgat cggctatgca aatgcgcacg
     4681 gaatgcaggc agccgtccat gccatcggag atgcctgtct ggaccgtgtt ttagatgcct
     4741 atgaaaaggc attaaaggag catccgagag aggatcaccg ccatgggatt gttcactgcc
     4801 agattacccg tccggatcag cttgagcgga ttgcccgctt aaagcttcat gtgtatgccc
     4861 agagtatttt tcttgattat gataatcata ttgtaaaaga cagagtggga gaggaactgg
     4921 catcgaccag ctacagctgg aaaaccctta tgaaacaggg tgtttctgtc agcaacggta
     4981 cggactgtcc ggtggagctg cccgacgctc tggcttctat ggagtgtgcc gtgacccgca
     5041 ctaccataaa ggatcatgta ggaccctatc ttccagatga agcttttaca gtgcaggaag
     5101 ctcttgacag ctatacgatc cgtggggcag aaggaagctt tgaggagaag ataaaaggaa
     5161 agatctgtcc cggtatgctg gcagactttg tgatcctgga gcagtcgccc tttgaaacgg
     5221 ctccggaaca gcttcatacg ataccggttc atgctgcgta tctgggagga agaaaggtat
     5281 ttggataaat atggaaatga cgaaaaagga actggcctta aagctgatcg agcgtctgaa
     5341 gaaagcctat ccggaggctt cctgtaccct ggattataat caggcgtgga agcttttggt
     5401 aggcgtccgt ctggcggccc agtgtacgga tgagcgggtc aacattgtaa cggagaagct
     5461 gtacaaaaag tttccggatg tggatgccct ggcggctgct tcgccggagg agattgagga
     5521 gatcgtaagg ccctgcggcc tgggacggag caaggccagg gatataagcg cctgtatgcg
     5581 gatcttaaaa gagcaatatg gcggacaggt gcctgaaacc tttgatgatc tgttgaagct
     5641 tccgggtgtg ggacgtaaaa gcgccaatct gatcatgggt gacgtatttg gaaagcctgc
     5701 cattgtaaca gatacccatt gcatccgtct ggtgaaccgt atgggattgg tagagggcat
     5761 aaaagatcct aaaaaggtgg aaatggctct ctggaagctg attcctccaa aggaaggcag
     5821 tgatttctgc caccgtctgg tattccacgg aagagcagtc tgcagtgcga ggacaaagcc
     5881 ggactgcgaa cactgctgcg tatcagacct gtgcgccagg aatggggtct gaaagaaaag
     5941 agggaaaact tcctttctgc ctgcatatag ttttacaaaa agcaaagagg cagaaacatg
     6001 ctggaagatg aatggattta tgtagtagaa gaaggggata cagtggatga gatagccgca
     6061 aaaaccggaa ggccggttgg agaactgatc tgggccaacc agctggaata tccctaccgc
     6121 ctggcagtgg gccagtctct ttatgtgggg gcagaaggca gtataggggc cggaagtccc
     6181 cttctttatt cctttggata tgcctatcct tttattgatc gggatacatt ggatgaaacc
     6241 ctgccatttc ttacagatat ttacattttc tcctatggtt ttacggcaga gggggatttg
     6301 gtctctcctc atatcgagga tacgccggtc atccagtccg ctcggactgc gggcgtccgt
     6361 ccggtgatgg tactcacgcc tctgggcccg gacgggcggt ttaataaccg gctgatcaca
     6421 gagcttcttt cgaatgaccg ggccgtgcgc cggctggtct ggcagctgat ggctgtgata
     6481 gagcaaaagg agtatgaggg actggatatt gattttgaat atgtgccggc cggtgacagg
     6541 gagcggtttg cggagtttgt ccgcctctgt gcaggggtgt ttagggcatt ggggattttt
     6601 gtcagtgctg ccctggcgcc aaagacctcc gatgaccagc cggggcttct ctatgaaggc
     6661 attgattacc gggccatcgg cgaagcggc
//
LOCUS       DBMDUH010000144         6653 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_4825_length_6653_cov_4.952686, whole genome shotgun sequence.
ACCESSION   DBMDUH010000144 DBMDUH010000000
VERSION     DBMDUH010000144.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 6653)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6653)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6653
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4825_length_6653_cov_4.952686"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            191..1321
                     /locus_tag="ACZPCG_10850"
     CDS             191..1321
                     /locus_tag="ACZPCG_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016288024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HIR1285590.1"
                     /translation="MNPSNVYFTDFHTIAFGDSLPTKLKKLIKKAGIENLDLDGKFVA
                     IKMHFGELGNISYLRPNYARAVVDVVKELGGKPFLTDCNTMYPGSRKNALEHMECAWE
                     NGFTPLTVGCPIIIGDGLKGTDDVDVPVEGGEYVKAAKIGRAVMDADVFISLTHFKGH
                     ESTGFGGAIKNIGMGCGSRAGKTEQHSSGKPHITPELCRGCRKCQKECANNGLIFNED
                     TRKMEVNLDGCVGCGRCLGACNFDAIAFNDNAANALLNCRMAEYTKAVIDGRPNFHIS
                     LIVDVSPNCDCHGENDIPILPNIGMFASFDPLALDQACVDACLAAAPMPGSQLARNMA
                     KKDFVDHHDHFRNSTPESEWKSCLEHAEKIGLGSREYTLIKM"
     gene            complement(1423..2520)
                     /gene="trpS"
                     /locus_tag="ACZPCG_10855"
     CDS             complement(1423..2520)
                     /gene="trpS"
                     /locus_tag="ACZPCG_10855"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HIR1285591.1"
                     /translation="MGKIILTGDRPTGRLHVGHYVGSLKRRVELQNSGQFDEIYIMIA
                     DAQALTDNADNPEKVRQNIIEVALDYLACGLDPEKSTLFIQSQIPELCEMTFYYMDLV
                     TVSRLQRNPTVKSEIQMRNFETSIPVGFFTYPISQAADITAFKATTVPVGEDQAPMIE
                     QTREIVHKFNSVYDGSLVEPEILLPDNKACLRLPGIDGKAKMSKSLGNCIYLSDSEEE
                     IKKKIMSMYTDPNHLRVQDPGQVEGNPVFIYLDAFCTDAHFEKYLPEYKNLDELKAHY
                     MRGGLGDVKVKKFLNNVIQDELRPIRERRKEFAKDIPAVYRILEEGSIKAEKKAAETL
                     AEMKRAMKINYFEDKELIAEQAARFKSESEA"
     gene            complement(2628..3719)
                     /locus_tag="ACZPCG_10860"
     CDS             complement(2628..3719)
                     /locus_tag="ACZPCG_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIR1285592.1"
                     /translation="MQELKVTANEAGQRLDKLLTKYLNQAGKGFLYKMMRKKNITLNG
                     KKCEGAERLEEGDVIRLFLADETIAKFSQITADLSFAPSASKVPQALKTPQTLNGRPL
                     SESSTVCCKKTGKENSAQAVKLDIIYEDSHILIVNKPSGMLSQKAKESDISLNEHILN
                     YLIQSGHLPVEQLRTFKPSICNRLDRNTSGLVAAGVSLAGLQGMNRIFKDRSIHKYYQ
                     CLVKGEVKEKQLITGFLKKDEASNKVEIHSKQTADSVPIMTEYVPLKSNGKVTLLQVT
                     LITGRSHQIRAHLASIGHPILGDFKYGDRRVNERVRSNYKITSQMLHSFRLVMPEDLP
                     APLHYLGKKEFSAPLPESFLRVMKGEGLA"
     gene            complement(3745..4536)
                     /locus_tag="ACZPCG_10865"
     CDS             complement(3745..4536)
                     /locus_tag="ACZPCG_10865"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR1285593.1"
                     /translation="MMIAVVTGATSGMGRECAIQLWEQFKGLDEIWAIGRRKERLEQL
                     KDFIGIPVRTFDLDLTEEKDLERLKAALTQAGPQVKFLVNAAGFGKIGGVRELSVSDQ
                     TGMAELNCTALTAVTSMVLPFMPNNSRILQFASSAAFLPQPGFAVYAATKAYVLSYSR
                     ALNRELKDRGISVTAICPGPVKTEFFDIAQTTGEIPLYKQFVMAKPDRVVKKAIRDSV
                     SRRELSVYGWTMKAFYLLTKLLPHRLILDVMERIEYAPKDSRWES"
     gene            complement(4545..6488)
                     /locus_tag="ACZPCG_10870"
     CDS             complement(4545..6488)
                     /locus_tag="ACZPCG_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HIR1285594.1"
                     /translation="MIHDYLPVTRQDMETRGWDQCDFVYVTGDAYVDHPSFGTAIISR
                     LLEAHGYRVGIISQPDWKTKESIQTFGRPRLGFLVSAGNMDSMVNHYSVSKKRRKEDA
                     YTPGGVMGKRPDYAVVVYCNLIRSVYKDVPIIAGGIEASLRRLAHYDYWSNRLKRSIL
                     LDAQADLISYGMGEHSIIEIADALDSGLDVKDITFIDGTVYKTKQLENVYDYKLLPDY
                     EELVRDKKRYAESFFIQYSNTDPFSGKRLVEPYDGKLFVVQNPPSKPLTKQEMDDVYA
                     LPYMRSYHPSYEALGGVPAIREIKFSLISNRGCFGACSFCALTFHQGRIIQARSHESL
                     VEEAKRLTEEPDFKGYIHDVGGPTANFRFPACEKQLTKGACPSKQCLFPEPCKNLKAD
                     HSDYVALLRKLRAIPKVKKVFIRSGIRFDYLLADTSRKFLKELCEYHVSGQLKVAPEH
                     VSDAVLNRMGKPKNAVYRRFVKEYKEMNERLGKDQYLVPYLMSSHPGSTMKEAVELAE
                     YLRDLGYMPEQVQDFYPTPSTISTCMYYTGYDCRTMEKVYVPVNPHEKAMQRALIQYR
                     DPKNYDLVTEALRIAGRTDLIGYDKKCLVRPGGTKDYRSKNSSGGSKGGKGTNSRPVQ
                     KTDKGQPSGKKKAIRNIHKKKGR"
     gene            complement(6501..>6653)
                     /locus_tag="ACZPCG_10875"
     CDS             complement(6501..>6653)
                     /locus_tag="ACZPCG_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HIR1285595.1"
                     /translation="LVFEDVPAGIMAGKRAGMTVYAVEDTFSKAMRKEKEALADGFIC
                     DFRELL"
BASE COUNT         1627 a         1700 c         1511 g         1815 t
ORIGIN      
        1 taaatagttt tcctccaccc cagccgaccc catagctgta ttctgacatt tttattatat
       61 agctttaagt tttctatgta aatcgaaata ttgcgaaaat cccatcattg ccatttcttc
      121 ccctttgctt tataataaaa aaggaactgt aaacccttac ataaaaagca aatgattacg
      181 aggtgaacca atgaatccgt caaacgttta tttcacagat tttcacacaa ttgcctttgg
      241 cgacagtctt cctaccaagt taaaaaagct gatcaaaaag gctgggattg aaaacctgga
      301 tctggatgga aaatttgttg cgatcaagat gcatttcgga gagctgggaa atatcagcta
      361 cctgcgtccc aattatgcca gggccgttgt tgatgtagta aaagagctgg gcggaaagcc
      421 cttccttaca gactgtaata ccatgtatcc gggtagcaga aaaaatgccc tggagcatat
      481 ggagtgcgca tgggaaaacg gctttactcc tttgaccgtg ggatgtccca tcattatcgg
      541 agacggcctt aagggaaccg atgatgtaga tgtgccggta gaaggcggcg aatatgtaaa
      601 agctgcaaag atcggacggg cagtgatgga tgctgacgta tttatcagcc tgacccattt
      661 taaaggccac gaatccacag gatttggagg cgctatcaaa aatatcggta tgggctgcgg
      721 ctccagagcc ggaaagaccg aacagcacag cagcggaaaa ccccatatta cccctgagct
      781 ttgcagaggc tgccgcaaat gccagaagga atgtgccaac aatggcctta tcttcaatga
      841 agacacccgc aagatggagg tcaatctgga cggctgcgta ggctgcggac gctgtctggg
      901 agcctgcaac tttgacgcca ttgcctttaa tgacaacgcg gccaatgccc ttctcaactg
      961 ccgcatggct gaatacacca aagctgtaat cgacggacgg ccaaacttcc atatttccct
     1021 gatcgtggat gtatccccca actgtgactg ccatggtgaa aatgatatcc cgatccttcc
     1081 aaatatcggc atgttcgcat cctttgatcc tctggcctta gaccaggcct gcgtggacgc
     1141 ctgtctggca gccgctccca tgcccggaag ccagctggca agaaatatgg caaagaagga
     1201 ttttgtggat caccatgacc atttccgcaa ttccactcct gaatccgagt ggaaaagctg
     1261 cttagagcat gcggaaaaga tcggactggg aagcagggaa tacaccttga tcaaaatgta
     1321 atatgtgatc cgcaagaaac aacaaaacag gagcatatca ggctttttgc agccggatgt
     1381 gctcctgttt gactgtggtg cgcctgacgg cacacgtttc atttatgctt ctgactcgct
     1441 cttaaatctg gctgcctgct ctgcgatcag ttccttgtcc tcaaagtaat tgatcttcat
     1501 ggcgcgcttc atctctgcca gtgtttctgc tgcctttttc tcagctttga tgcttccctc
     1561 ttccaggatc ctgtatacag cgggaatatc ctttgcaaac tcttttctgc gctcacggat
     1621 ggggcgaagc tcatcctgga tcacattgtt taaaaatttc ttaactttta cgtctcccag
     1681 tccgcctctc atgtaatgag cctttaactc atccagattt ttgtactccg gcagatactt
     1741 ttcaaagtga gcgtccgtac agaaagcatc cagatagata aatacaggat tcccttccac
     1801 ctgtcccgga tcctgaactc ttaagtggtt gggatcggta tacatggaca tgatcttctt
     1861 tttaatctcc tcctcagaat cagacaggta aatgcagttt ccaagggact tgctcatttt
     1921 tgcctttccg tcgattcccg gcagacgcag acatgcctta ttgtctggaa gaagaatctc
     1981 aggctctacc agacttccgt catagacgga gttgaattta tggacgatct ctctggtctg
     2041 ctcgatcatg ggcgcctggt cctctcctac cggcacggtc gtcgccttaa aggcagtaat
     2101 gtccgcagcc tggctgatgg gataggtgaa aaagccaacc ggaatgcttg tttcaaagtt
     2161 gcgcatctga atctcagatt ttaccgtagg atttctctgg agacgggaaa ccgtcacaag
     2221 atccatatag tagaaggtca tctcacagag ctcagggatc tgggactgga taaacagagt
     2281 agacttctca ggatccagac cgcaggccag gtaatcaagg gctacttcga tgatattctg
     2341 gcgtactttt tccggattat ctgcattgtc tgtcaatgcc tgagcgtctg cgatcataat
     2401 atatatttca tcaaactgac cggaattctg cagttccact ctgcgtttta atgagcctac
     2461 ataatgtcca acatgaaggc ggcctgtagg acggtcgccg gttaatatga tttttcccat
     2521 tgtattttat cctctcatct ccagttaaaa accggagttt ataataaata agcatacccc
     2581 aaaggtgata ttctgtcaag cacggaagag gctgtttcca gccttgctta tgccagtcct
     2641 tcgcctttca taactcttaa aaatgactcg ggaagaggcg ctgaaaactc ctttttgcca
     2701 agataatgaa gaggcgcagg aagatcttca ggcataacca gacggaagga atgaagcatc
     2761 tgggaggtga ttttgtagtt tgaccgcaca cgttcattga cccttctgtc accatactta
     2821 aaatcaccta aaataggatg gccgatggag gctaaatggg cccggatctg atggctgcgc
     2881 ccggtaatca gcgttacctg caaaagggtc acttttccgt ttgatttaag gggaacatac
     2941 tccgtcataa tgggtacgct gtctgctgtc tgctttgaat ggatctctac tttgttggaa
     3001 gcttcatcct tctttaaaaa gcctgtgatc agctgttttt ctttcacctc tcctttaacc
     3061 aggcactgat aatatttgtg gatgctcctg tctttgaata tgcggttcat cccctggagt
     3121 cctgccagtg atacgcctgc cgccacaagt ccgctggtat tccggtccag acggttgcag
     3181 atagagggct taaaggtacg cagctgctca accggcagat ggccggactg gatgaggtag
     3241 tttaagatat gttcatttag ggaaatatca ctctcttttg ctttctgaga aagcatgccg
     3301 gagggcttgt tgacgatcaa aatatgactg tcctcataga tgatatccag ctttactgcc
     3361 tgggcgctgt tttcctttcc cgtctttttg cagcagaccg tgctgctttc tgatagcggc
     3421 cgaccgttca acgtctgcgg ggtttttaaa gcctgaggga cttttgatgc agacggggcg
     3481 aaggatagat cggctgtgat ctgggaaaat ttagcaatcg tctcatccgc cagaaacagg
     3541 cggatcacat ctccttcctc cagtctctcg gctccctcac acttctttcc gttcagcgta
     3601 atgttttttt tacgcatcat tttatataaa aagccttttc ctgcctgatt cagatatttg
     3661 gttaaaagct tgtctaaccg ctgtccggcc tcatttgccg tgaccttcaa ttcctgcatg
     3721 gtctttttct ccttatatcc tgatttagga ttcccaccgg ctgtcctttg gcgcgtattc
     3781 gatccgttcc attacatcca ggatcaggcg gtggggcaat agctttgtaa gcagatagaa
     3841 agccttcatg gtccagccat aaacagaaag ctcacgtctg gatacactat cccggatggc
     3901 tttttttacc acccggtccg gctttgccat cacaaactgc ttataaaggg ggatttcacc
     3961 tgttgtctgg gcaatatcaa aaaattccgt tttcacaggt ccggggcaga tggctgttac
     4021 agaaatcccc cgatctttta attcccggtt taaggctctg ctatagctta gtacgtaggc
     4081 ttttgtcgcc gcatagaccg caaagcccgg ctgtggcaga aaggcggcgc tggaggcaaa
     4141 ctggaggatc cggctgttat ttggcataaa gggaagaacc atagacgtaa ctgccgtaag
     4201 ggccgtgcag ttaagctctg ccattcctgt ctggtctgat acagaaagtt cccttacacc
     4261 gccaatcttt ccaaaaccgg cggcatttac aagaaatttt acctgaggcc cggcctgcgt
     4321 aagagctgcc tttaatcgtt caaggtcttt ttcttctgta agatccagat caaaggtgcg
     4381 tacaggaata ccgatgaaat cctttaactg ctccagacgc tcctttctgc gtcctatggc
     4441 ccaaatctca tccaggcctt taaactgctc ccacagttga atggcgcatt ctctccccat
     4501 tcctgaggtg gctcctgtca ctacagcaat catcataata agccctacct tcccttcttt
     4561 ttatggatat tgcgaatggc tttcttcttt cctgacggct ggcctttgtc tgttttctgg
     4621 acaggccggc tgtttgtccc ctttcctccc tttgatccgc cggaactgtt tttgctcctg
     4681 tagtccttcg ttccacccgg acgcaccaga cacttcttat catagccgat cagatccgtc
     4741 cttccggcaa tccgcagcgc ctctgtcaca agatcgtagt ttttcgggtc gcggtactgg
     4801 atcagcgccc tctgcatggc tttttcatgt ggattgaccg gcacatagac tttttccatg
     4861 gtacggcagt catagccggt atagtacata caggtggaaa tagtagaggg cgtggggtaa
     4921 aagtcctgta cctgctccgg catatagccc agatcccgca gatattctgc cagctccacc
     4981 gcttccttca tggtagaacc ggggtgggag gacatcagat agggaaccag atactgatcc
     5041 tttcccagac gctcattcat ctccttatat tccttgacaa acctgcggta gaccgcattt
     5101 ttcggctttc ccatacggtt taacacagca tcagaaacgt gctccggagc tacttttaac
     5161 tgtccgctta cgtggtattc gcacagctcc tttaaaaatt ttctgctggt gtccgctagc
     5221 aggtagtcaa aacggatccc ggaacggata aatacctttt tcaccttcgg aatagcccgc
     5281 agcttcctca aaagggccac ataatcggaa tgatctgctt tcagattttt acatggctca
     5341 ggaaacaggc actgctttga gggacaggct cccttcgtaa gctgcttttc acaggccggg
     5401 aaacggaaat tagccgtagg cccgcctaca tcatggatat agcccttaaa atcaggctct
     5461 tctgtcaaac gctttgcctc ttccactaag gattcatggc ttctggcctg aatgatccgt
     5521 ccctgatgga aggtaagggc gcagaaactg caggcgccaa agcagccccg gttgctgatg
     5581 aggctgaact tgatctcacg gatagccgga acaccgccaa gagcctcata agaggggtga
     5641 tagcttctca tatacggcag agcatagacg tcatccatct cctgcttcgt aaggggcttt
     5701 gaaggcggat tctgcaccac aaaaagtttc ccgtcatagg gctccaccag acgttttcca
     5761 gaaaatggat ctgtattgct gtactggata aaaaagcttt ccgcatacct ttttttatca
     5821 cggacaagct cctcataatc cggcagaagc ttgtagtcat agacattttc cagctgcttt
     5881 gtcttataaa ccgtaccgtc aataaaggta atatccttca catccagacc gctgtctaag
     5941 gcatctgcta tctctatgat ggagtgctct cccattccat aggaaataag atccgcctgg
     6001 gcgtctaaaa ggatcgagcg ctttaaccgg ttggaccagt agtcatagtg ggccagacgg
     6061 cgcaggctgg cttcaatgcc tccggcgata ataggaacat ccttgtagac ggagcggatc
     6121 aggttgcagt aaaccacaac cgcataatcc ggccgttttc ccatgacgcc tcccggagta
     6181 taggcatcct ctttccggcg ctttttcgat acggaataat ggttcaccat ggagtccata
     6241 tttcccgcag aaactaaaaa gcccagcctg gggcgtccga atgtctgtat actttccttt
     6301 gttttccagt ccggctggga aatgatcccc acacggtagc cgtgggcctc caaaagcctt
     6361 gaaataatgg cggttccaaa ggaagggtga tctacatagg cgtcgcctgt cacatagaca
     6421 aaatcacact gatcccagcc tcttgtttcc atatcctgcc gggtaaccgg caaataatca
     6481 tgtatcatac gctctgctcc ttataaaagc tctctaaaat cacagataaa gccgtctgcc
     6541 agagcttcct tttctttccg catggctttg gaaaatgtat cttccactgc gtacaccgtc
     6601 atcccggctc tctttccagc catgattcca gccggaacat cctcaaacac cag
//
LOCUS       DBMDUH010000145         6643 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_4831_length_6643_cov_6.965301, whole genome shotgun sequence.
ACCESSION   DBMDUH010000145 DBMDUH010000000
VERSION     DBMDUH010000145.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 6643)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6643)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6643
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4831_length_6643_cov_6.965301"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="ACZPCG_10880"
     CDS             <1..504
                     /locus_tag="ACZPCG_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008975356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR1285596.1"
                     /translation="GAQERRRRAGTENVPGIVGFGKAVQLAMDEMEERTKKEVEMREY
                     LMEKVLREVPFTRINGSRTSRLPNNINFAFQFIEGESLLIKLDMAGICGSSGSACTSG
                     SLDPSHVLLAIGLPHEIAHGSLRLTLSEENTMEEMDITADKIKEIVAYLRELSPLYED
                     YMRKHSR"
     gene            537..974
                     /gene="nifU"
                     /locus_tag="ACZPCG_10885"
     CDS             537..974
                     /gene="nifU"
                     /locus_tag="ACZPCG_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HIR1285597.1"
                     /translation="MYTEKVMDHFEHPRNVGELENPSGVGTVGNPKCGDIMRIYLDID
                     ENQIIRDVKFKTFGCGAAVATSSMATELVKGKTVQEAMQVTNKAVMEALDGLPPVKVH
                     CSLLAEEAIHAALWDYAQKNGITIEGLEKPKDNIEEEEEEEDY"
     gene            971..2047
                     /gene="mnmA"
                     /locus_tag="ACZPCG_10890"
     CDS             971..2047
                     /gene="mnmA"
                     /locus_tag="ACZPCG_10890"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HIR1285598.1"
                     /translation="MSKKVVVGMSGGVDSSVAAWLLKEQGYDVIGVTMQIWQDEDTEV
                     QEAEGGCCGLSAVDDARRVAMELGIPYYVMNFKEEFKCRVMDYFAQEYLAGRTPNPCI
                     ACNRYVKWESLLQRSLAIGADCIATGHYARIAKLENGRFALETSVTAAKDQTYALYNL
                     TQDQLSRTLMPVGDYTKDEIRQMAQKLGLPVASKPDSQEICFIPDHDHASFIENYTGK
                     ESKPGNFVDLKGNVIGRHKGITHYTVGQRKGLNLAMGHPVFVTALRPETNEVVIGDSQ
                     DVFTSTVRCNRLNWMAVDGLHGRETRVKAKIRYNHGGAMCTIREIENGEVECRFDEPV
                     RAAAPGQAVVFYENGYVVGGGTIL"
     gene            2044..3342
                     /locus_tag="ACZPCG_10895"
     CDS             2044..3342
                     /locus_tag="ACZPCG_10895"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HIR1285599.1"
                     /translation="MRQRGGAERAKTVRMFFWKRGVLAAAMAALILSGCSEYESSEEL
                     RAQAGTEAQADGEVQAAAEVRTSKQTETGADISGRSQSRTRAAVPEFETVSDRVYVNT
                     EGGSVRIRSACDTADASNILANVPDGTSLSRTGKNADWTRIQWEGKTGYISTAYISET
                     AKQKKTQISAGSSSQGGAIAENGRQVLLDPNWDYAEFAKITSGTAVLYQSQTAERKNL
                     TICVNAGHGTKGGASVKTQCHPDGSPKVTGGTTGAGAVTAAAVSGGMTFDDGTPESSV
                     TLSMALILKDKLLAKGYDVLMIRETEDVQLDNIARTLIANNASDCHIALHWDSTSSNK
                     GAFYMSVPNNAAYRNMEPVKSNWQKHNALGESLVAGLKEKGVKIFSGGSMEMDLTQTS
                     YSTVPSVDIELGDKASDHSQAALKQLADGLAAGIDQYFAG"
     gene            3499..4335
                     /locus_tag="ACZPCG_10900"
     CDS             3499..4335
                     /locus_tag="ACZPCG_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6612 family protein"
                     /protein_id="HIR1285600.1"
                     /translation="MRKMKRFFAVLLSAALLCPMTAYADNSEDAKALYHQVSETQKSI
                     TDMNAFADFKISIGGSILEDTGVDAMNLRMEMNMKMNHMTEPQQMRYMMYSRTTMDGM
                     EEPMIISAYYQDGCYYQDSMGQKVKMPMDIGTMMEQSMASAMAFEDEFENYLSNFRLW
                     DEGENKVIGFVAEADQMNEYFQMVMSSTGMSGLLDTAGITMGDVIYEYVIDPAGNCIK
                     MRMKMDMAMEADGESITMKLDGDIGIADPGQPVEVPTVPDPEAYQDFETLIAEAVQAE
                     QQ"
     gene            4474..5292
                     /locus_tag="ACZPCG_10905"
     CDS             4474..5292
                     /locus_tag="ACZPCG_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002605628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HIR1285601.1"
                     /translation="MSDTQNTVAAVKKELLTRLGHDADIFVLISLCTREPYVVCDPET
                     FDDEVFLYLNEEEAKTAAAGLAEQKIPVSVAKVNGKDMLVFFTSLYAMGVNALIVEMN
                     GEKASMQVGDIVKRKSPDDMPQGTVWVENPSLHLTAIYLAQEMRRPAAEAEAEARAER
                     IKELNEELTADFRKGSYIFAIQKEEKGIPLVKMKDGQLFQPAFTDILEFQKFNRDNKL
                     RPVVVEAEKVPTVLAAEAGGIILNVLGVNLPLTVNRPGVQRPEAPAAEAVPEQA"
     gene            5289..>6643
                     /locus_tag="ACZPCG_10910"
     CDS             5289..>6643
                     /locus_tag="ACZPCG_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR1285602.1"
                     /translation="MKKTVVGLLAHVDAGKTTLSEAILYGAGAIRRLGRVDDRDTFLD
                     TFELERARGITIFSKQAVFSMGDTEVTLMDTPGHVDFSAEMERTLQILDYAVLVINGA
                     DGIQGHTETLWRLLKRYQIPAVLFVNKMDQAGTDKERLMAELKDRLDEGCVDFGGWQA
                     DGGEWNPGTDERESFAENLAVCDEAVLETYLETGEIGREEIIRMTAGRKLFPVFFGSA
                     LKMKGIESFLRGLFEIVRAPEGSSSFGAKIYKISRDSQGNRLTHLKVTGGVLRVKDAL
                     IGEEKVNQIRIYSGSKYETVQEAQTGTVCAVTGLLSSAPGQGLGAEAASELPVLEPVL
                     TYRILLPEGTDVHKSLRDLRQLEEEEPELHILWEEATGEIKAQVMGEVQIEILKTLIR
                     ERYGLEAEFGTGNIVYKETILEPIEGVGHFEPLRHYAEVHLLLEPGEPGSGLQFDTVC
                     S"
BASE COUNT         1796 a         1411 c         1950 g         1486 t
ORIGIN      
        1 ggagcccagg agcgcagacg cagggcggga acggaaaatg ttccaggcat tgtgggcttt
       61 ggaaaagcgg ttcagcttgc catggatgag atggaagagc ggaccaaaaa ggaagttgag
      121 atgcgggagt atcttatgga aaaagtgctg cgggaggttc cctttacccg gatcaacggt
      181 tcccgtacca gccgtctgcc gaataatatc aattttgcct tccagtttat tgagggtgag
      241 tcccttttga tcaagctgga tatggccgga atctgcggtt ccagcggatc ggcctgtacc
      301 tcaggctcct tagatccttc ccatgtgctt cttgccattg gtcttcccca tgagattgcc
      361 cacggctctc tgcgtctgac cttaagtgag gaaaatacca tggaggaaat ggatataaca
      421 gcagataaga ttaaggaaat cgtagcatat ttaagagagc tttcccctct gtatgaggat
      481 tatatgagaa aacacagccg ttaaagggcg aaaatgacga acaaggagaa gagacgatgt
      541 atacagaaaa agtaatggat cattttgaac atcccagaaa cgtaggagag cttgagaatc
      601 caagcggcgt aggaacggtg ggaaatccca agtgcggcga catcatgcgt atttatctgg
      661 atattgacga aaaccagatc atccgggatg taaagtttaa aacctttggc tgcggagctg
      721 ccgtagcaac cagcagcatg gcaacagagc ttgtaaaggg aaaaacggtg caggaagcca
      781 tgcaggttac aaataaagct gtgatggaag cacttgacgg actgcctcct gttaaggtac
      841 actgttcatt gttagcagag gaggccattc acgccgccct ctgggattat gcacagaaaa
      901 acggtattac cattgagggt ctggaaaagc ccaaggataa tatcgaagaa gaggaagaag
      961 aggaagacta ttgagcaaaa aagttgtagt aggcatgtcc ggaggagtcg attcctccgt
     1021 ggcagcctgg ctgttaaagg agcagggtta tgatgtgatc ggagtaacca tgcagatatg
     1081 gcaggatgag gataccgagg tccaggaggc agagggaggc tgctgcggtt tgagcgcagt
     1141 agatgacgcc cgcagagtcg ccatggaact gggtatccct tattatgtta tgaattttaa
     1201 agaggaattt aagtgcaggg taatggatta ttttgcccag gagtatctgg cggggcgcac
     1261 ccccaatcca tgtatcgcct gcaaccgcta tgtgaaatgg gagtccctgc tgcagagaag
     1321 ccttgcgatc ggggcggact gcattgcaac aggccattat gcaaggattg caaagctgga
     1381 aaacggccgt tttgcccttg aaacctctgt gacggcggca aaggatcaga cctatgccct
     1441 ttataatctg acccaggatc agctgtcaag gacattgatg ccggtaggag attacacaaa
     1501 ggatgagatc cgccagatgg cccagaagct gggccttccg gtggcatcca agccggacag
     1561 ccaggaaatc tgttttattc cggatcacga ccatgcctcc tttattgaga attatacagg
     1621 caaagaatca aagcccggaa attttgtaga tcttaaggga aatgtgatcg gccgccataa
     1681 gggcattacc cattatacgg tcggccagag aaagggctta aatctggcta tgggccatcc
     1741 ggtctttgtc acggccctgc gtccggagac aaacgaggtg gtgatcgggg acagtcagga
     1801 tgtatttacc agtaccgtgc gctgcaatag gctcaactgg atggctgtgg acgggcttca
     1861 cggcagagag acgagagtga aggccaaaat ccgctacaat cacggcggag ctatgtgcac
     1921 tatccgcgaa atagaaaatg gagaggtaga atgcaggttc gatgagcctg tacgggctgc
     1981 ggctccggga caggctgtgg tattttatga aaatggctat gtagtgggag gaggaactat
     2041 cctatgaggc agaggggcgg agcagaacgc gcaaaaacag tccgaatgtt tttctggaag
     2101 agaggtgttt tagccgctgc aatggccgcg cttattttat caggctgttc agagtatgag
     2161 tccagtgaag aactgcgggc acaggccggt acagaggcgc aggctgatgg agaagtacag
     2221 gctgctgcag aagttcggac atccaaacag acggagaccg gagcggatat ttccggccgc
     2281 tcccagtcca gaacacgggc tgcagtgccg gaatttgaaa cggtttccga tcgggtttat
     2341 gtaaatacag agggcggctc tgtgcggatc cgcagcgcct gtgatacggc ggacgcctct
     2401 aatatcctgg caaatgttcc cgatggcaca tccctttccc gaacgggtaa aaatgcagac
     2461 tggacaagga tacagtggga ggggaagaca ggatacattt ctactgcgta tatttctgag
     2521 acagcaaaac agaaaaaaac tcagatttct gccggcagca gctctcaggg cggcgccata
     2581 gctgaaaatg gcaggcaggt tttattggat ccgaactggg attatgcaga gtttgcaaag
     2641 attacctccg gaacggcggt tctctatcag agccagacgg cagagagaaa gaaccttacc
     2701 atctgcgtca atgcaggtca tggtaccaaa ggaggcgctt ctgtaaagac tcagtgtcat
     2761 ccagacggca gccccaaggt cacaggaggc actacgggag cgggagctgt gacagccgcg
     2821 gcggtatccg gcggcatgac ctttgacgac ggtacgcctg agagcagcgt taccctttcc
     2881 atggcgctca ttttaaagga taagctgctg gcaaagggct atgatgtgct gatgatccgt
     2941 gaaacagagg atgtccagct ggataatatc gccagaacac ttatcgccaa taatgccagc
     3001 gactgccaca ttgcgcttca ctgggattcc acctccagca ataagggagc cttttacatg
     3061 agtgttccaa acaatgccgc ttaccggaat atggagccgg taaaatcaaa ctggcagaag
     3121 cataacgcgc tgggagaaag cctggttgcg gggttaaagg aaaagggggt taagatcttt
     3181 tccggcggct ccatggagat ggatctgacc cagacctcct attctaccgt tccgtctgtg
     3241 gacattgaat tgggagataa ggcctctgac cattcccagg ccgcgttaaa acagctggcg
     3301 gatggcctgg cagccggtat agaccagtat tttgccggat agggaacaga tgaaaaaata
     3361 tcagaattct taagagaaaa gatgaaatcg taagaaatat atttgcgttt tacatggaca
     3421 gcagagctgt tttggagtac aatagcagta tcatcagtga caatttaaga ggaatttctt
     3481 taccgaggag gttacgctat gagaaaaatg aaacgtttct ttgctgtcct gctatcagcc
     3541 gcgctgcttt gccctatgac cgcctacgca gataattctg aggatgcaaa agctctttat
     3601 caccaggtat ccgaaactca gaaatccatt acagacatga acgcctttgc tgattttaag
     3661 atttccattg gaggcagtat cctggaggat acaggcgtag atgccatgaa tctgcgtatg
     3721 gaaatgaata tgaagatgaa ccatatgaca gagcctcagc agatgcgcta catgatgtat
     3781 tcccgtacga ctatggacgg catggaagag ccgatgatca tttccgcata ttatcaggac
     3841 ggctgctact atcaggattc catgggacag aaggtaaaga tgcctatgga tataggaact
     3901 atgatggagc agagcatggc atccgctatg gcctttgagg atgagtttga gaattattta
     3961 tccaatttcc gtttatggga tgagggagaa aataaggtga tcggctttgt ggctgaagcc
     4021 gaccagatga atgaatattt ccagatggtg atgagttcta ccggcatgag cggactgtta
     4081 gatacggcag gcattaccat gggcgatgtg atctatgaat atgtgattga tccggcagga
     4141 aactgcatta agatgcgcat gaagatggat atggcaatgg aagcagatgg tgaaagcatc
     4201 accatgaagc tggacggcga tatcggcatc gcagacccgg gccagcccgt agaggttccc
     4261 acagttcctg atccggaggc atatcaggat tttgaaactc tgatcgcaga ggctgtacag
     4321 gcagagcaac agtaaaaaga caggaaattt aaaagcaggt tatgaagaac cggcgcagac
     4381 caaaagcgcc ggttcttttt cctgaactaa ttgcaccctt ttgcggaatg tggtacaatt
     4441 ttacaagaat gtgttgataa ggagaaaatg aaaatgagtg atacgcaaaa caccgttgca
     4501 gctgtaaaga aggagcttct tacccggctg ggacatgacg ctgatatttt tgtactgatt
     4561 tccctgtgca caagggaacc gtatgtggta tgtgatccgg aaacctttga tgacgaggta
     4621 ttcttatact taaatgagga ggaggcaaaa acagcagcag ccggcctcgc agagcagaag
     4681 atccctgttt ccgttgcaaa ggtaaacggt aaggatatgc tggtattttt taccagtctt
     4741 tatgctatgg gcgtgaatgc cctgatcgtg gagatgaacg gggaaaaggc ttccatgcag
     4801 gtaggagata ttgtaaagcg caagtctccg gatgatatgc cccagggaac cgtgtgggtg
     4861 gaaaatccat cccttcatct gactgccatc tatctggctc aggagatgag acggccggca
     4921 gctgaggcag aagcagaggc gcgggcagaa cggatcaaag agctcaatga ggagctgaca
     4981 gccgatttcc gcaagggcag ctatattttt gccattcaga aagaggagaa ggggattcct
     5041 cttgtaaaga tgaaggacgg acagctgttc cagcctgctt ttacagatat tctggagttc
     5101 cagaaattta accgcgacaa taagctgcgc ccggttgtgg tggaggcaga aaaggtgcct
     5161 accgtactgg cggctgaagc aggcggcatt attttaaatg tgctgggagt aaaccttcct
     5221 ttgactgtaa accgtccggg ggttcagaga ccggaagcac ctgcggctga ggctgtgcct
     5281 gagcaggcat gaaaaaaaca gttgtaggtc tgttggccca tgtagacgcc ggaaagacta
     5341 ccctgtcgga ggctatcctt tacggcgccg gagccatccg caggctgggg agagtggacg
     5401 acagggatac gtttttagat acctttgagc tggagcgggc cagagggatt accattttct
     5461 ccaaacaggc cgttttttcc atgggcgata cggaagtgac ccttatggat acgccgggcc
     5521 atgtggattt ttcagcagaa atggagcgca cccttcagat cctggactat gccgttctgg
     5581 tgatcaatgg agcggacggc atccagggcc atacggaaac gctgtggagg ctgctaaagc
     5641 gctatcagat tccggccgtt ttgtttgtca acaaaatgga tcaggcgggt acggataagg
     5701 agcgcctgat ggcagagcta aaggatcgtc tggatgaggg ctgtgtggat ttcggaggct
     5761 ggcaggcaga tgggggagaa tggaatcccg gaacagacga gagggagagc tttgcagaga
     5821 acctggcggt ctgtgatgag gcggtgctgg aaacatatct ggagacagga gagattggca
     5881 gggaggagat tatccgcatg acagccggcc ggaagctttt tcctgtattt ttcggatcag
     5941 cccttaagat gaaggggatc gagagctttt taagggggct ttttgaaatt gtccgggctc
     6001 cggagggaag ctcttctttt ggtgccaaaa tctataaaat atccagagac agtcagggaa
     6061 accgtctgac ccatttaaag gttaccggag gagtccttcg tgtgaaggat gcgctgatcg
     6121 gggaagaaaa ggtcaaccag atccggattt attccggatc aaagtatgag acggttcagg
     6181 aggcgcagac aggtacggtc tgcgcagtta ccggccttct ttcctctgct ccgggacagg
     6241 ggctgggagc agaggctgct tctgagcttc cggtactgga accggtgctg acctatcgca
     6301 ttcttcttcc ggaaggtacg gatgtgcata aaagcctccg ggatctgcgc cagctggagg
     6361 aggaggagcc ggagcttcac atcctctggg aagaggcgac cggtgagatc aaggctcagg
     6421 ttatgggcga agtacagatc gagattttaa aaacgctgat ccgtgagcga tatggtcttg
     6481 aggcagagtt cggcacggga aatatcgtgt ataaggaaac aatcctggag cccatagagg
     6541 gcgtgggcca ttttgaacct ctgcgccatt atgcggaggt gcatcttctt ctggagcctg
     6601 gggaaccggg aagcggcctg cagtttgaca cagtctgcag cga
//
LOCUS       DBMDUH010000146         6580 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_4873_length_6580_cov_4.542522, whole genome shotgun sequence.
ACCESSION   DBMDUH010000146 DBMDUH010000000
VERSION     DBMDUH010000146.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 6580)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6580)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6580
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4873_length_6580_cov_4.542522"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(69..584)
                     /locus_tag="ACZPCG_10915"
                     /pseudo
     CDS             complement(69..584)
                     /locus_tag="ACZPCG_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591717.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
     gene            complement(601..2658)
                     /gene="recG"
                     /locus_tag="ACZPCG_10920"
     CDS             complement(601..2658)
                     /gene="recG"
                     /locus_tag="ACZPCG_10920"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HIR1285603.1"
                     /translation="MSGHQPVSALKGIGEKTGKLFEKLGVITIDDLISYYPRAYDTYE
                     EPVPIGQLKEQTVMSVASVLSKPPDLLRTGKVQMVSAMIRDLTGTLQLAWFNMPYMRA
                     NVKSGQMYVFRGRVVKKRGRLMMEQPEVFTREAYDALEHSMQPVYGQTRGLSNKTIVK
                     AQKQALEMRRLEREYMPAVLRRKYELAEINYALEHIHFPSDEKELLFARRRLVFDEFF
                     MFLVGVRRLKEHREKEMSSWGLKECLEVLKLRERLPYALTGAQERTLKEVFSDMAGGR
                     VMNRLIQGDVGSGKTIIAILALLQAAYNGCQGALMVPTEVLARQHFESMQKLFTENGI
                     DKVPVLVTGSMTAKEKRLAYEKIASHEADIIVGTHALIQEKVVYDRLALVITDEQHRF
                     GVGQRELLSNKGNTPHVLVMSATPIPRTLAIILYGDLDISVIDELPAGRQEIKNCVVD
                     ISYRPKAYAFIQKQVEMGHQAYVICPMVEPSEMIEAENVVDYTKTLKKVFPESISVEY
                     LHGKMKAKEKNKIMERFAAGEIDVLVSTTVIEVGVNVPNATVMMIENAERFGLAQLHQ
                     LRGRVGRGKAQSFCIMVNGNGGKDTMERLDIMNRSNDGFYIASEDLKLRGPGDIFGVR
                     QSGELEFKLADIFTDANLLKQVSEEVNAILDQDPGLEGEEYRELKQKIEQYLGDREEH
                     LNL"
     gene            complement(2655..3536)
                     /locus_tag="ACZPCG_10925"
     CDS             complement(2655..3536)
                     /locus_tag="ACZPCG_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR1285604.1"
                     /translation="MIIGALEAGGTKMVCALGKEDGSIIDQISIPTTTPEETIPKIIE
                     YFKDKGIEALGIGAFGPVGVDPASENYGYILETPKLAWRNKDLLGSLKKGLKVPMAID
                     TDVNGSCLGEVTYGCAKGLNSVIYITIGTGVGVGVWVNGGLLHGMLHPEGGHILLNRH
                     PEDTKGGVCPSHPNCFEGFASGPSIEARWGKKAVDLKEQSEVWELESFYIAQALADYI
                     MILSPQKIILGGGVMHQEQLFPLIRRKVKELVNGYICSKELDDIDNYIVPASLNDDQG
                     IMGAIRLGLNALEQEKS"
     gene            complement(3558..4265)
                     /locus_tag="ACZPCG_10930"
     CDS             complement(3558..4265)
                     /locus_tag="ACZPCG_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007711469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HIR1285605.1"
                     /translation="MNDEFRMEQRTVNQAGESLGRYTAKTFGWMFAGLLITLIVAISM
                     YVTGAFIYVIRIPAWNFLLLAAELILVISLSARLEKMSVTAAKSMFFLYSAVNGIVFS
                     LYFLMFDLLSLWIAFGITAMFFGVMALIGYFGNINFSALRPFMTGGLIFLFVFWLLAM
                     FIDLSAFETMVCGVGIFLFLIITAYDAKKIQAYYAYYGQNSEMAAKASVFSALQLYLD
                     FINLFIYILRFVGNRKD"
     gene            complement(4288..5991)
                     /locus_tag="ACZPCG_10935"
     CDS             complement(4288..5991)
                     /locus_tag="ACZPCG_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HIR1285606.1"
                     /translation="MGISTIDAGMVKKAFLAGAKGLEAKKDWINELNVFPVPDGDTGT
                     NMTMTIMAAAKAVAELEDPDMEQLAKAISSGSLRGARGNSGVILSQLLRGFTKEIQGT
                     ASIDVTILANAMVRGTETAYKAVMKPKEGTILTVAKGMADKAIEMAAQTDDIEIFAKA
                     VIEEGDYVLSQTPEMLPVLKQAGVVDSGGQGLMQVVKGAFDGLTGKCSDFSFEDAGIL
                     KPSSEKALQSAGASRTDIDTADIRFGYCTEFIIKLEKEYSQKDEDELKKYLGSIGDSL
                     VVVSDDEIVKIHVHTNHPGLAFEKGLTYGSLSRMKVDNMREEHEERVIQDSERLAKEQ
                     AAQPNPAEEETGERKPYGFITVSCGDGLSEIFKGIGADYLIEGGQTMNPSTEDMLNAI
                     KKVNADHIFILPNNKNIIMAANQARDLTEDKEIIVIPSKTVPQGITALVNFIPDLTPE
                     ENLENMTAEMERVQTAQITYAVRNTSIDGMEIHEGDIMAIGDHGMLAVDTSVLGAAKA
                     ALEAMLNEDSELVTIYYGSDVTEADAEAFRKQAEEEFPDKEIELQYGGQPIYYYMISA
                     E"
     gene            complement(6005..6361)
                     /locus_tag="ACZPCG_10940"
     CDS             complement(6005..6361)
                     /locus_tag="ACZPCG_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HIR1285607.1"
                     /translation="MKGRISNKMGEIQISPEVIALYAGTIAVECFGIVGMAAVSMKDG
                     LVKLLKRESLTHGITVDIRENRISIDFHVIVSYGVSISAVADNLIESVKYRVEEFTGM
                     EIEKINIFVEGVRVID"
BASE COUNT         1620 a         1789 c         1306 g         1865 t
ORIGIN      
        1 atgtgatttt ctgaacataa tacactagaa tgttttgcgt aaaatgtcaa aatatagaaa
       61 atatctgtct attccactac aactactttt ccgatccgct gtttttctgc ttttacatca
      121 tctgcatatc ccaaagcagc cattccatat acgatatgat ctgccggaat gccaaaatca
      181 tttaaaattt cacgaatgga aggctcatcg cagattccct gaagctgatt gatctacaca
      241 gagccaatcc caagagaatg agcggccaga aagatatttt ccatagcgca ggcattgtcc
      301 tcttttccat gaggattatc cctctgattg gaaggaatga tcaaaatctc cggctgatac
      361 atatcataat tttcccggcc cagtatcttt cctgcggcct ctgccagcct ctgcaccttt
      421 tttctgtttt ttaccacggt aaactgccag gtctgttttc ccattccgct gggagcatag
      481 agagccgcct taaggatctg atccagatct gtctgcggaa tctcctgaac cttaaacttt
      541 cttgtacttc ttcttgtaag gattgtctgc attgtttcgt tcatcttcac cgtctccttt
      601 ttaaagattt aaatgttcct ccctgtctcc aaggtactgc tcaatttttt gcttaagctc
      661 cctgtattcc tccccttcaa gaccaggatc ctgatccagt atcgcattta cttcctctga
      721 tacctgtttt agaagattgg catccgtaaa gatatctgcc agtttaaact ccagttcccc
      781 gctctggcgt acgccgaaga tatctcccgg cccgcgaagc tttaaatctt cagaagcaat
      841 ataaaaaccg tcattggaac ggttcataat atccagccgt tccatggtat ccttaccgcc
      901 gtttccattc accataatac agaaggactg ggctttccca cggcccacgc ggccccggag
      961 ctgatgaagc tgggccagtc cgaagcgctc tgcattttca atcatcatga cagtggcatt
     1021 ggggacattc accccaacct ctatgacagt ggtagatacc agcacatcaa tctctccggc
     1081 agcaaagcgt tccatgatct tattcttctc ttttgccttc atttttccgt ggagatattc
     1141 aacagaaatt gattcaggaa atactttttt cagcgttttc gtgtaatcca ccacattttc
     1201 cgcctcgatc atctcgcttg gctctaccat gggacagatc acataggcct gatggcccat
     1261 ttccacctgc ttctggataa atgcatatgc ctttggccgg taactgatat ccaccacgca
     1321 gtttttaatt tcctgccgtc ccgcaggcag ctcgtcgatc acggaaatat ccagatcccc
     1381 ataaaggata attgccagag ttcttggaat gggggttgcg ctcataacaa gtacatgggg
     1441 cgtatttccc ttgttgctta aaagctctct ctggcccaca ccgaaccggt gctgctcatc
     1501 cgtaatcact aacgccagac ggtcatagac caccttttcc tggatcagcg catgggttcc
     1561 cacgataata tccgcctcat gggaagcgat tttttcataa gccagccgct tttcctttgc
     1621 ggtcatggag ccggtaacaa gcacaggaac tttgtcaatt ccattttccg taaacagctt
     1681 ttgcatggat tcaaaatgct gtctggcaag cacctccgta ggtaccataa gagctccctg
     1741 gcagccgtta taagcggcct gcaaaagggc aagtatggca ataatggtct ttcctgagcc
     1801 tacatctccc tgtatcagcc ggttcatcac ccggcctccc gccatatcag aaaatacctc
     1861 ttttaaagtc ctctcctgag ctcctgtaag ggcataggga agcctttccc tgagttttaa
     1921 aacctccagg cattccttta agccccagga agacatttct ttttccctgt gctccttcag
     1981 ccgtctgacg cctacaagaa acatgaaaaa ttcatcaaac acaaggcggc gtcttgcaaa
     2041 aagaagctcc ttttcatcag atggaaaatg gatatgctcc agcgcatagt taatctctgc
     2101 cagctcgtat tttctgcgga gaacggccgg catatattcc cgttccagcc ggcgcatttc
     2161 aagcgcctgt ttctgggcct tcacaatggt tttattgctc agccctctgg tctgcccgta
     2221 taccggctgc atggaatgct ccagcgcatc ataggcctct ctggtaaaaa cctccggctg
     2281 ttccatcatc aggcggcccc gttttttcac cacccgcccc cggaatacat acatctggcc
     2341 gctttttaca tttgcccgca tatagggcat attaaaccag gcaagctgca gtgttcctgt
     2401 cagatcacgg atcatcgctg ataccatctg cacctttccg gtgcgcagaa gatccggagg
     2461 ctttgaaagc acagaagcca cgctcattac ggtctgttcc tttaactgtc caatgggaac
     2521 cggttcttca taggtgtcat atgctctcgg ataataggaa atgaggtcat caatggtgat
     2581 gacccctagc ttttcaaaca gttttcctgt tttctctcca attcccttta aagcgcttac
     2641 aggctgatgg ccgctcatga tttttcctgc tcaagggcat ttaatcccag gcggatcgcg
     2701 cccatgattc cctgatcgtc atttaagctt gcaggaacaa tgtaattgtc aatatcatcc
     2761 agctctttgg agcagatata gccgtttaca agctccttta ccttccttcg gattaacggg
     2821 aacagctgct cctgatgcat aacgccgccg cccaggataa tcttctgggg cgacaaaatc
     2881 ataatataat ctgccagagc ctgagcaata tagaagcttt caagctccca tacttcagac
     2941 tgctccttta aatcaactgc cttttttccc catctggctt caatggaagg gccgctggca
     3001 aagccctcaa agcagtttgg atggctgggg cagactcctc cctttgtatc ttcgggatgg
     3061 cggtttaaca ggatatggcc tccctccgga tgaagcatcc cgtggagaag tcctccattt
     3121 acccacacgc ctacgccaac tcccgttccg atggtaatat aaatcacact gtttaaacct
     3181 ttggcgcagc cataggtcac ttctcccaaa caggagccgt ttacgtccgt atcaatggcc
     3241 atgggaacct taagcccttt tttcaggcta cccagaagat ccttatttct ccaggccagc
     3301 ttaggagttt ccagtatata tccgtagttt tcagaagcag gatccactcc caccgggcca
     3361 aaggctccga tgccaagggc ttcgatcccc ttatccttaa aatactcaat gatctttgga
     3421 atggtttcct ccggtgtggt agtcggaatg gagatctggt ctataatact cccgtcttcc
     3481 tttcccaggg cgcagaccat cttggttccc cctgcttcca gtgctccgat tatcattttg
     3541 gctttatccc cctcttatca gtcttttctg tttcctacaa aacgcagaat atagataaac
     3601 aggttaatga aatccagata cagctgcagt gctgagaaaa cggaagcctt tgctgccatc
     3661 tcggaattct gtccgtaata agcatagtat gcctggattt tctttgcgtc gtaagctgta
     3721 ataattaaaa agaggaaaat tcccacaccg cagaccattg tttcaaaagc gcttaaatca
     3781 ataaacatgg ctaacagcca gaaaacaaac aggaagatca ggccgcctgt cataaaagga
     3841 cgcagagcgg aaaaattgat atttccaaaa tacccgatca gtgccatcac cccgaaaaac
     3901 atagctgtaa ttccaaatgc gatccacagg gaaagaaggt caaacatcaa aaaatacagg
     3961 ctgaatacga taccatttac agcagagtac aggaaaaaca tacttttcgc tgcagttaca
     4021 gacatctttt ccagtctggc agacagtgaa attactaaaa tcagttcagc cgccagcaga
     4081 aggaagttcc acgccggaat ccggattaca tagataaatg ctcctgtaac atacatactg
     4141 atggcaacga tcaatgttat aagcagtccc gcaaacatcc agccaaaggt tttagcagtg
     4201 tatcgcccca gagattcccc cgcctgattg actgtcctct gttccattct gaattcatcg
     4261 ttcatacttg cttttctcct ttcatttcta ctctgcagat atcatataat aataaatagg
     4321 ctgaccgccg tactgcagtt cgatttcctt atccgggaat tcctcttcgg cctgctttct
     4381 gaaggcttct gcatctgcct cggtcacatc gcttccataa taaatagtaa ccagctcgct
     4441 gtcctcattt aacatcgcct caagagccgc cttcgctgct cctaaaacag atgtgtccac
     4501 tgccagcatc ccatgatcgc cgattgccat aatatcgccc tcatggattt ccattccgtc
     4561 gatggaggta tttcgcaccg cataggtaat ctgggctgtc tgcacccgct ccatctcggc
     4621 tgtcatattt tccagattct cctccggcgt cagatctgga atgaaattca ccagagccgt
     4681 gatgccctga ggcacagttt tggaaggaat aacgataatc tccttatcct ccgtcagatc
     4741 acgagcctga ttggccgcca tgataatatt tttattgttc gggagaatga aaatgtgatc
     4801 ggcattgacc tttttaatgg cgtttaacat gtcctccgtg ctggggttca tggtctgtcc
     4861 gccttcaatg aggtaatcgg ccccgattcc cttgaaaatc tcgcttaagc catcgccgca
     4921 ggatacggtg atgaatccat agggcttccg ctcgccggtt tcctcctctg cagggttggg
     4981 ctgggcagcc tgctcctttg ccagacgctc tgaatcctgg atcacccgct cctcatgctc
     5041 ttcccgcata ttgtcaacct tcatacggga aagggagccg taggtaagtc ccttttcaaa
     5101 ggccagtccc ggatgattgg tgtgaacatg gatctttacg atctcatcat ctgaaaccac
     5161 taccagagaa tctccgatag agcccagata cttcttaagc tcatcctcat ccttctggct
     5221 gtattccttt tcaagcttaa tgataaattc ggtacagtaa ccgaagcgga tatccgctgt
     5281 gtcaatatcc gttctggaag ctccggcaga ctgaagggct ttttcagaag aaggttttaa
     5341 aatgcctgcg tcctcaaagg agaaatcact gcattttccc gtaaggccgt caaaagctcc
     5401 ctttactacc tgcatcaggc cctgaccgcc ggaatccact acgcctgcct gctttaatac
     5461 gggaagcatc tccggtgtct ggcttaaaac gtagtcgccc tcctcaatga ctgcctttgc
     5521 aaaaatttca atatcatccg tctgagcggc catttcgatg gctttgtccg ccatgccctt
     5581 ggcaaccgta agaatggtgc cttcctttgg cttcataact gccttgtagg cggtttctgt
     5641 accgcgcacc atggcatttg ccagaatggt cacatcaata gacgctgtgc cctggatctc
     5701 ctttgtaaag cctctcaaaa gctgtgacag gataactccg gaatttcctc tggcgcctct
     5761 taaagagccg gaagaaatag cctttgccag ctgctccata tccgggtctt ccagttctgc
     5821 taccgctttg gcagccgcca tgatcgtcat cgtcatattc gtcccggtat ctccatccgg
     5881 taccggaaaa acattcagct cattgatcca gtctttcttg gcctcaaggc cctttgctcc
     5941 tgccagaaag gcttttttta ccatcccggc atcaattgta cttataccca cgggttgctt
     6001 cctcctaatc aatcactctg acaccttcta caaagatatt gatcttttcg atctccatgc
     6061 cggtgaattc ttctactctg tacttcacac tttcgatcag attgtcagcc actgctgaaa
     6121 tgctgacccc atacgatacg atcacatgaa agtcaatgga tatcctgttt tctctgatat
     6181 ccacagtgat gccgtgtgtc aggctctctc ttttcagcag cttcacaaga ccgtctttca
     6241 tacttacggc cgccataccc acgataccaa agcactctac tgcaatggtt cctgcatata
     6301 aagcaatcac ttccgggctg atctggatct cgcccatctt attgctgatt cgtcccttca
     6361 tatacacgcc tcctgttttc atattaagga cagtatacac tatttcccga aaaaaagaaa
     6421 taaatttttg ttttttcact tgcaaaaatt gaaaacatct ggtatcatta cttttgttgt
     6481 ggatttttct attttctaca agaaatccaa gtaacgttta aggaggtgca atagtatggc
     6541 taagtgtgca atctgtgaaa aggcagctca tttcggaaac
//
LOCUS       DBMDUH010000147         6539 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_4897_length_6539_cov_4.660454, whole genome shotgun sequence.
ACCESSION   DBMDUH010000147 DBMDUH010000000
VERSION     DBMDUH010000147.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 6539)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6539)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6539
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4897_length_6539_cov_4.660454"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="ACZPCG_10945"
     CDS             complement(<1..920)
                     /locus_tag="ACZPCG_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015572954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HIR1285608.1"
                     /translation="MENTKKITWYTLAFMAFSTVWGFGNVLNGFVYFNGIQVIFSWIL
                     MFALYFVPYALMVGELGSAFKTSGGGVSSWIHETISPKFAYYAGWTYWACHVTYIASK
                     GSGGLKALSWMFFQNGTTYDQFPTIWIQLATLAALLFFCWVASRGLNPLKKLATIAGT
                     SMFVMSILYILMMFAAPAINPNGGYVTMEFSKETLIPQFNLGYFTSLSILVFAVGGCE
                     KISPYVNKVKDPSRGFPKGMIALAIMVMVCAILGTIAMGMMFDPAVINESSESFNSYV
                     SNGAYWSFQKLGEYYHVGNLLLIIYAACNA"
     gene            1084..1557
                     /locus_tag="ACZPCG_10950"
     CDS             1084..1557
                     /locus_tag="ACZPCG_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285609.1"
                     /translation="MKKWLRYTVLILLLWLLFVILCLVIPPLFHKEAGYGPGEDAEGD
                     SFSTPERILCIDDNKEALIWRLRLIGAAKERIILTTFDFRDEESGQDIMAALWEAEIQ
                     TTGFWDIIRKVITRIGICWSMERSRDKVVLFRRWKHIFMRFGISPAVRSLMQKAV"
     gene            1584..>2076
                     /locus_tag="ACZPCG_10955"
     CDS             1584..>2076
                     /locus_tag="ACZPCG_10955"
                     /inference="COORDINATES: protein motif:HMM:NF024491.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HIR1285610.1"
                     /translation="MKERYPEAFDRIYSKEDWEKATMETRGIELWTNPTEPENKEPVV
                     WERMIAAMEGEEDILVQTPYMICSRKMYEDLRSVCEKGARVDIVINAVESGTNPFGCT
                     DYLNQKKKICQTGVHIYEYLGDQAQHTKSLAVDCPELNASIREQARPLKEKSRHMSPD
                     GRTE"
     assembly_gap    2077..2176
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2177..2375
                     /locus_tag="ACZPCG_10960"
     CDS             <2177..2375
                     /locus_tag="ACZPCG_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HIR1285611.1"
                     /translation="LAVDCPELNASIREQARPLKEKSRHMSPDGRTEDGQNYEPREQD
                     MAKRAVYALLRIVISPFRHVL"
     gene            complement(2384..3679)
                     /locus_tag="ACZPCG_10965"
     CDS             complement(2384..3679)
                     /locus_tag="ACZPCG_10965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1285612.1"
                     /translation="MNTLRMLHYFNFVMATIFVVFYFYQIGYLLLGLWKRHTKETDVS
                     HALHRYAAVISARNEANVIGGLIASLKAQDYPSELLDIYVIADNCTDNTAQVALEAGA
                     KVYRRFNRQLIGKGYALDYLFKCLSASGKDVYDGYFVFDADNYVDPNFVREMNNTFDG
                     GHYAALTSYRNSKNFGANWISAGYGLWFLREARFLNFPRMLLGTNCAISGTGFLVSGE
                     VIREKGGWPFHLLTEDIEFSVNCAIDGKVIGYCDKAVIYDEQPVDFKQSWRQRLRWSK
                     GFYQVDWHYSLDLIRGCFRPGRKGFSCYDIFMTVAPGMFFTLAALIINVVMLGAYLTE
                     PGYIARMIVEENIFFLGRTLGMFYVSLLIYGAITMAVEWKQIAMSAGRKILYTFTFPI
                     FMFTYIPISAVALVKKVEWKPICHGNTAGELIPNKGQGR"
     gene            3965..5860
                     /locus_tag="ACZPCG_10970"
     CDS             3965..5860
                     /locus_tag="ACZPCG_10970"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase domain-containing protein"
                     /protein_id="HIR1285613.1"
                     /translation="MAYKFDAGFLKRIIERMQSSLYITDPVTHEILYTNGYIKDAFHC
                     EKPEGKYCWQILQKDMKGPCSFCKIPDLYNKELGEAVVWREHNEVSGRDYINYDWLEE
                     WNGRRYHIQNSVDITSFLQLSIEASIDELTGLLNRNAGKNHLSEVLSSLKEDGLCTVA
                     LCDLNGLKWVNDTYGHLEGDRFLKVTACTMQEGMEASDFAFRLSGDEFILVFQNKDSD
                     QAAGILEEILARLKERRQSVGIDYEATFSYGLVTVNGGHRLSVSDVLSVADMQMYIRK
                     RDYHILSGKQAARRLSKEAATRGNAFSYHKDQLLDALSESIDDYIFVGNLKTGEFRYT
                     WQMAEDFGLPGQVVKNAASFWKRRIHPDDARMFLDSNQEIADGRADRHAIVYRAKAAD
                     GTWKHLLCRGRMYRDGNGRPELFAGVIRNLDKEHEKSLMAPKPGIARTTVGQKELDSF
                     LTTAIIRASKGSADKTLGEKELLFSFRFNLTRERLEELWGLDEKLNRESSDRSLSFLL
                     GYINSHIASEDDRKRFGLRYNREEILRDITLKRAVPSFEFREKDREGRIRWARMDFHA
                     VRQNDGDLLLYGYARNIDEQKKRELALEYKAGTDIVSGFYDQTTVRLMIQSILENKTA
                     KTVFMLR"
     gene            5869..>6539
                     /locus_tag="ACZPCG_10975"
     CDS             5869..>6539
                     /locus_tag="ACZPCG_10975"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR1285614.1"
                     /translation="MDDFSKTGKNWSRGEKEEVLRQASTILRSSLPPLSVAGRLEQDV
                     FLIFYFDISSADEIREEAGRLRRLLCRTYQAGNELLYMSWSAGLCCSCEETVTYDQLY
                     HRSLKALERAKYEGKDQMKVYGERSETAERQKLSWELLRECFKIGDTGIIPPAFGEAA
                     AAQRLSFIPAEEASLLWERLGARSGQEQVFEAEKREEAAYGELEGLLEETSYQFPLLW
                     TGCWG"
BASE COUNT         1840 a         1326 c         1700 g         1573 t
ORIGIN      
        1 atggcattgc aggctgcata gataattaac agcaggtttc ctacatggta gtattcaccc
       61 agtttctgga aggaccagta agcgccgttg gatacatagg aattaaagct ttcagagctt
      121 tcgttgatca cagcaggatc gaacatcata cccatagcga tggttccaag gattgcgcag
      181 accataacca taatggcaag agcgatcata cccttgggga aaccgcggga aggatccttt
      241 accttattta cataagggga gatcttctca cagccaccta cggcaaatac caggatggat
      301 aaggaggtaa agtatcccag gttaaactga gggatcagtg tctccttgct gaattccatg
      361 gtaacatagc cgccgtttgg attgatggca ggagctgcaa acatcatcag gatatacagg
      421 atggacataa cgaacatact ggttccggca atggttgcaa gcttttttaa tgggtttaag
      481 ccgcggcttg ctacccagca gaagaataaa agcgcagcca gtgtagccag ctggatccag
      541 atggttggga actggtcata agtagttccg ttctggaaga acatccagct taatgccttt
      601 aagccgccgc ttcccttgct ggcgatatag gtaacgtggc aggcccagta agtccagcct
      661 gcgtagtagg caaatttcgg gctgatcgtt tcgtggatcc aggagctgac gccgccgccg
      721 gaagttttaa aagcagatcc cagctcgcct accataaggg catagggcac aaaatacagt
      781 gcgaacatca ggatccagct gaaaataacc tgtataccgt taaagtatac aaagccgttg
      841 agtacatttc cgaagcccca gaccgtagaa aaggccatga atgccagggt gtaccaggtg
      901 attttctttg tgttttccat tgataacctt ccttttctaa aatgtttaac tttactagga
      961 tatcatattt ctatgaaata ttcaaatgaa agtaatattt ttttattaaa atttttaaga
     1021 atttttattt atctgtgata tacttctttt tataacatct gaaaatgaaa ggggaagctg
     1081 caaatgaaaa aatggcttcg atataccgtt ctgatattgc ttttgtggct tttattcgtg
     1141 atcctttgtc tggtgatccc gccgctgttt cataaagaag caggatatgg cccaggggag
     1201 gatgcagaag gagatagctt tagtacgccg gaacggattc tctgtattga tgataataaa
     1261 gaggctctga tctggagact gagactgatc ggggctgcta aagaacggat cattctcact
     1321 acctttgatt tcagagatga ggaaagcgga caggatatca tggcggcttt gtgggaggca
     1381 gaaatacaga caaccggttt ttgggatatt ataaggaaag ttataacgag gatagggatc
     1441 tgttggtcta tggagagaag ccgggacaag gtagttcttt tcaggcgctg gaagcatatt
     1501 ttcatgagat ttggaatcag ccctgctgta aggagtttga tgcaaaaggc ggtataggcg
     1561 ggctggagca gtgctgtgag cgggtgaaag agcggtatcc ggaagccttt gaccggattt
     1621 attcaaaaga ggactgggag aaggctacca tggaaaccag ggggattgaa ctgtggacga
     1681 atcccacaga gccggaaaat aaggagcctg ttgtatggga aaggatgatc gcagccatgg
     1741 aaggggaaga agatatcctg gtacagactc cctatatgat ctgcagcaga aagatgtatg
     1801 aggatcttcg ctctgtctgt gaaaagggcg ctagagtgga cattgtgatc aatgccgtag
     1861 aaagcggcac caaccccttt ggatgtaccg attatcttaa tcagaaaaag aagatctgcc
     1921 agactggagt ccatatttat gagtatctgg gagaccaggc gcagcatacc aaatcgctgg
     1981 cagtagactg ccctgaatta aacgcctcta tccgtgagca ggcacggcca ttaaaagaaa
     2041 aaagccgcca catgagcccg gatggccgga ctgaggnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnngctg gcagtagact gccctgaatt aaacgcctct atccgtgagc
     2221 aggcacggcc attaaaagaa aaaagccgcc acatgagccc ggatggccgg actgaggacg
     2281 gacaaaatta tgagccgagg gagcaggata tggcaaaaag ggctgtatac gcgcttcttc
     2341 ggatcgtaat cagtcctttc cgccatgtgc tgtaggggaa atcctatcgc ccctgtccct
     2401 tgtttggaat cagctctccg gccgtatttc catggcagat gggtttccat tctacctttt
     2461 tgacaagggc aacggcagag atcggaatat aggtaaacat aaaaatcggg aaggtgaagg
     2521 tataaaggat cttcctgccc gcagacatgg cgatctgttt ccattctacc gccatggtga
     2581 tagcgccgta gatcagaagg ctcacataaa acattccaag cgtccttcca agaaagaaga
     2641 tattttcctc cacgatcatc cgggcaatat agccgggttc tgtaagatag gcgcccagca
     2701 tgaccacgtt gatgatcagt gctgccaggg taaaaaacat tcccggtgct accgtcataa
     2761 aaatgtcata gcaggagaaa ccctttctgc ccggacggaa gcagccgcgg atgagatcca
     2821 ggctgtagtg ccagtcaacc tgataaaatc ccttagacca gcgcagtctc tgtctccagg
     2881 actgtttaaa gtctacgggc tgttcatcgt agataacggc cttgtcacag tagccgatca
     2941 cttttccatc aatggcgcag tttacagaaa attcaatatc ctcggtcaga agatggaagg
     3001 gccagcctcc cttttcacgg atgacttctc cggataccag aaacccggtg ccggaaatgg
     3061 cacagttggt tcccaaaagc atacggggga agttaaggaa gcgggcttcg cgcagaaacc
     3121 acagtccata tccggcagag atccagttgg ctccgaagtt tttggaattg cggtagctgg
     3181 tcagagccgc atagtggccg ccgtcaaatg tattgttcat ttcccgtacg aaattgggat
     3241 ctacataatt atcggcatca aatacaaagt agccgtcata aacatccttt ccggaagcgg
     3301 acagacattt aaacagataa tccaaagcgt agccctttcc gatcagctgg cggttaaagc
     3361 ggcggtatac cttggcgcct gcctctaagg ctacctgagc cgtattatcg gtacagttat
     3421 cggcgatcac atagatatca agaagctcgg aagggtaatc ctgggccttc aggcttgcga
     3481 tcaggccgcc gattacattt gcctcatttc tggcagaaat cacagctgca tagcggtgaa
     3541 gggcatggga gacatctgtc tcttttgtgt gccgtttcca cagacctaac agcagatagc
     3601 caatctgata gaaataaaac acaacaaaga tagtggccat tacaaaatta aaataatgaa
     3661 gcattcgcag tgtattcata tctaacctct ttacatgata tttggatcct gtttgtgata
     3721 cagaatcaca gtattagaat caatataaaa aaatagcata tttttaaaat aatacaagag
     3781 ttttaagcaa attcataaaa aattaatatt tacttcatat agtattcaat tatgaatgat
     3841 aaacagcgta tctgtctggt tcactattta cattacatgg aactacagat ataataaatg
     3901 acagaataca tgaactttca ggagtgaaag gcagggaaaa aagatggcag gggggtgcag
     3961 ctgtatggct tataaatttg atgcgggatt tttaaagagg atcatagaac ggatgcagtc
     4021 cagtctgtat attacggacc ccgttaccca tgagatcctt tataccaacg gatatatcaa
     4081 agatgcgttt cattgtgaaa agccggaggg gaaatactgc tggcagattc tacaaaagga
     4141 tatgaagggt ccctgttcct tctgtaaaat tcctgatctg tataacaaag aattgggaga
     4201 agctgttgtc tggcgggagc ataatgaggt ttcagggcgc gattatatca attatgactg
     4261 gctggaggaa tggaacgggc gcaggtatca catacagaac tctgtggata ttacttcttt
     4321 tcttcagctg tccatcgagg cctctattga cgaactgacc gggctcttaa accgaaatgc
     4381 gggaaaaaat catctaagcg aggttctttc ctccttaaag gaggacgggc tttgtacggt
     4441 agctctttgc gatctaaatg gattgaaatg ggtcaatgac acatatggcc atctggaagg
     4501 cgaccgtttt ttaaaggtta cggcctgtac catgcaggaa ggaatggagg cttctgattt
     4561 tgctttccgc ctcagcggag atgagttcat tctggttttc cagaataagg attctgatca
     4621 ggcagccggt attcttgagg aaatccttgc gcgcttaaag gagcgcagac agtccgttgg
     4681 catcgattat gaggcaacct tcagctacgg tctggtgact gtgaatggag gccacaggct
     4741 ctctgtttcc gacgttcttt ccgtagcgga tatgcagatg tatatacgca agagagatta
     4801 tcacatatta tccggaaagc aggcagccag acggctcagc aaagaagcgg cgacgagagg
     4861 aaacgctttt tcctatcata aagaccagct tttagacgcc ctgtcggaga gtattgacga
     4921 ttatatcttt gtaggcaatc tgaagacagg agagtttcgt tatacctggc agatggcgga
     4981 ggactttggg cttcccgggc aggtggtaaa aaatgcagct tccttttgga aacgcaggat
     5041 ccatccggat gacgcaagaa tgtttttgga cagtaatcag gagattgcag acggaagggc
     5101 agaccgccat gccattgtat accgggcgaa ggcagcagac ggcacatgga agcatctttt
     5161 atgcagaggg cggatgtaca gagatggaaa tggccgcccg gagctgtttg caggggtgat
     5221 ccgcaatctg gataaagaac atgaaaagag cctgatggct ccaaaaccgg gaattgccag
     5281 aactacggtg ggacaaaagg agctggattc ctttttgacg acggctatta tacgggcttc
     5341 gaagggaagt gcggataaaa ctctgggaga gaaggaactt cttttttcct tccgtttcaa
     5401 tctgaccaga gaacggctgg aggagctttg ggggttggat gagaagctta acagagagag
     5461 cagtgacaga agcctttcct tccttttagg ctacatcaac agccatattg ccagcgagga
     5521 tgacaggaag cggtttggac tcagatataa cagagaagag atcttgcggg atatcacttt
     5581 aaaacgggca gttccgtcct ttgaattcag ggaaaaggac agggaaggaa ggatccggtg
     5641 ggccagaatg gatttccatg cagtgcggca gaatgacgga gatcttcttt tatatggcta
     5701 tgccagaaac attgatgagc agaaaaagag agagctggcg ctggaataca aagccggaac
     5761 tgatattgtc agcggttttt atgatcagac aaccgtccgc cttatgatcc agagcatact
     5821 ggaaaataaa acagcaaaaa cggtattcat gctacgctga tcctggatat ggatgatttt
     5881 tcaaaaacag gaaaaaattg gtccagaggc gaaaaggaag aggttctccg tcaggccagc
     5941 acgatcctgc gcagcagcct tccgcccctt tccgtggcag gacggctgga gcaggatgta
     6001 tttctcatct tttactttga tatctcctca gcagatgaga tcagggagga ggctggcaga
     6061 ctgcgcaggc tgctgtgccg gacgtatcag gcggggaacg agcttttgta tatgagctgg
     6121 tcggcaggtc tttgctgcag ctgtgaggaa accgttacat acgaccagct gtatcacagg
     6181 agtttaaaag ctctggaacg ggctaaatat gagggcaaag accagatgaa ggtctatggg
     6241 gagaggagtg agacagcaga gaggcagaag ctttcctggg agcttttaag agaatgcttc
     6301 aaaattgggg ataccggtat tataccaccg gcctttggag aagcagcggc tgcgcagagg
     6361 ctttctttta ttccagcaga agaagcttct cttttatggg aacgtctggg tgcaagatct
     6421 ggacaggaac aggtgttcga agcagaaaag agggaggaag ctgcctatgg ggagctggaa
     6481 ggcctgctgg aagaaacatc gtatcaattc ccgcttttgt ggactggctg ctggggaga
//
LOCUS       DBMDUH010000148         6531 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_4905_length_6531_cov_7.021617, whole genome shotgun sequence.
ACCESSION   DBMDUH010000148 DBMDUH010000000
VERSION     DBMDUH010000148.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 6531)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6531)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6531
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4905_length_6531_cov_7.021617"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            142..312
                     /locus_tag="ACZPCG_10980"
                     /pseudo
     CDS             142..312
                     /locus_tag="ACZPCG_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585867.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
     gene            321..650
                     /locus_tag="ACZPCG_10985"
     CDS             321..650
                     /locus_tag="ACZPCG_10985"
                     /inference="COORDINATES: protein motif:HMM:NF014373.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HIR1285615.1"
                     /translation="MLVCMGAAIVMAMGHYMQEAFQTGDPARLGAQVISGIGFYSAGI
                     LATLAVYLIMARFKKLEYKFILEDVYCGVYVEFNGTVTISDIAQRISGYGLEIEEVQM
                     GKPGYGA"
     gene            849..1538
                     /gene="nth"
                     /locus_tag="ACZPCG_10990"
     CDS             849..1538
                     /gene="nth"
                     /locus_tag="ACZPCG_10990"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HIR1285616.1"
                     /translation="MPKIKRETKTARSQRVAHILEALDREYGTELICYLNHETPWQLL
                     IAVILSAQCTDARVNIVTADLFQKYPSLEAFAAADVKEMEQDIHSIGFYHNKAKNIIL
                     CARDLLERFSGEVPESLEDLTSLAGVGRKTANVIRGNVYHVPSIVVDTHVKRISRKLG
                     LTLEEEPEKVEYDLMKVLPKDHWILWNIHIITLGRTVCTARKPKCGECFLKEYCPSCE
                     EPVVFDGGKAR"
     gene            1535..2314
                     /locus_tag="ACZPCG_10995"
     CDS             1535..2314
                     /locus_tag="ACZPCG_10995"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006782714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HIR1285617.1"
                     /translation="MMTSYIALDLETTGLESKTEKITEAAALKVIDGTVRERFVTLIN
                     PGRPISEKITQLTGITDEMVRTAPLMENVIGELLAFCGGLPLLGHNILFDYRFLKQAA
                     VNSQLECEFEAVDTLTLCRHLMPPDEKKNLSASCTWFQIPQSAAHRAEADAESAHLLY
                     EKLKALYGKEREELFVPCPLIHKAKREQPATKRQKEYLQDLIKCHRIDITLQTDTLSR
                     SEASRLIDQIILQRGRLTEHSLSSPYNGFVGGSGRRKGSQD"
     gene            2503..4182
                     /locus_tag="ACZPCG_11000"
     CDS             2503..4182
                     /locus_tag="ACZPCG_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HIR1285618.1"
                     /translation="MKNKMWKTALTVLAAAGAVWAFPGTAMAAPVLPDGIRVQGQDLA
                     GKTCGEARDVIETYEEELGNCPVLLTLDGQEKETTAKELGLYWSNENQIEETLKEYAG
                     GSLIRQYMVKKDLARAPVELAVETAVDPEKVTAFVDNHSQDILTQPQNASIRRENGAF
                     VITEAVSGRAVDTEATVQALNEALARQQEGTVRADAVITEQEPEITSEELASIQDVLG
                     TCTTDFSSSGAARSTNLSVGAAKINGRVMMPGEVLSGYECLQPFTTSNGYKTAAAYEN
                     GKVVDSIGGGVCQIATTLYGASLEAEVEIVQRQNHSMIVTYVEPSMDAAIAGTYKDIK
                     IKNNYSTPIYVEAYTSGKKLTFTLYGKETRPEGRKVEYISETIGTTSPGEPQLIVDNT
                     LAPGAKVRVQSSHTGLRSRLWKVVTVDGVETERTLLNKDTYNASKAVYRVGPEAPVNA
                     PVQTDPAGGQTDGAGENTSPESPGAENVAGGPGMPGESGTVPGGGPGAPAGGTSGSGA
                     ASGGPGAPEGAGGSSGAVSGGGPGASPEAGGSAAAPGGGPGVPALSGEVAQ"
     gene            4179..4772
                     /locus_tag="ACZPCG_11005"
     CDS             4179..4772
                     /locus_tag="ACZPCG_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HIR1285619.1"
                     /translation="MRTAGQGPGGFEKRDGHINPGQELIFAGSAGLAGARRILEAKKE
                     ILSRRFAPSFLRELKEEEYSVKSWLDQATAEKKSPVTAWEFAGEGGVLAALWNFSGIY
                     CTGLDVDLRLFPVHQAAIEVCELFELNPYRLYSENCVLLAADSGSRLVRELKQAGIPA
                     AVIGWAEKGIARKLRHGEEAAGYLERPQPDELLKLGL"
     gene            4816..5298
                     /locus_tag="ACZPCG_11010"
     CDS             4816..5298
                     /locus_tag="ACZPCG_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HIR1285620.1"
                     /translation="MREKILAVIEKNSRIDIKDLAVLLGESEIAVANEIADMEKEHII
                     CGYHTLINWDNTSEEKVVALIEVKVTPQRGMGFDKIAERIYQYSEVNAVYLMSGSFDF
                     TVFIEGKTMRQVAQFVSDKLAPMEAVLSTATHFVLKKYKDHGTIISEPVQDERMLITP
                     "
     gene            5295..6464
                     /locus_tag="ACZPCG_11015"
     CDS             5295..6464
                     /locus_tag="ACZPCG_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008726189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR1285621.1"
                     /translation="MRNPLSDKIVSIPPSGIRKFFDIVNEMEGAISLGVGEPDFDTPW
                     HIREEGIYSLEKGRTFYTSNAGLKSLKDEISRYLSRRFDVSYDSDKEIMVTVGGSEAI
                     DAAMRAMLNPGDEVLIPQPSYVSYVPCAVLADGVPVVIELEEKDHFKLTPEKLLEKIT
                     DKTKILVLPFPNNPTGAIMERHELEEIAKIVVEKDIFVISDEIYSELTYTDNHVTIAS
                     FPGMKERTVLINGFSKSYAMTGWRLGYAAAPEIILKQMLKIHQYAIMCAPTTSQYAAV
                     AALRDGDNDVEMMRESYNQRRRYLLNAFKEMGIDCFEPLGAFYTFPNISRFGMTSEEF
                     ATALLKEEKVAVVPGTAFGACGEGFVRISYAYSLKSLKEALGRIERFVKNHDGKA"
     gene            6470..>6531
                     /locus_tag="ACZPCG_11020"
     CDS             6470..>6531
                     /locus_tag="ACZPCG_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HIR1285622.1"
                     /translation="MMNIVLLEPEMPSNTGNIGRT"
BASE COUNT         1752 a         1403 c         1912 g         1464 t
ORIGIN      
        1 tggcccatct ggagccggcg ggggacggaa gttttctgct gtttgactat aaaaaccgcg
       61 acccgctgac ctataatgca aggatggttc gggttgagat ttgctaaaat gtatttttga
      121 gaaggagaga cagaggctga tatgccaacg attaaagata tagcagcccg tgccggagta
      181 tcccacggcg cggtttctaa tgtattaaat aaaaggggaa atgtgagtgc tgaaaagatc
      241 cagttagtgg agcaggccgc cagggaactg ggctaccagc tcaatgccca ggcccagcag
      301 ctgcgggcag gagaacctat atgctggtat gtatgggagc cgccattgtg atggctatgg
      361 gccactatat gcaggaggcg tttcagaccg gagatccggc caggctggga gctcaggtaa
      421 tcagcggcat tggcttttac agcgcaggaa ttctggctac gcttgcagta tatctgatta
      481 tggcacggtt taagaagctg gaatataaat ttattctgga ggatgtgtac tgcggcgtct
      541 atgtagagtt taacggtaca gtgacgattt ctgatattgc acagaggatc tcgggttatg
      601 gcctggagat tgaagaggtt caaatgggaa agccgggata tggtgcttaa gcagatggag
      661 gccatagagg gcgtccggta cgcaaaatac atattttaaa gattatggaa aaggcgctgc
      721 ctgtctggga ttatctcaga tgggtggtgc tttttgcaaa ggctacaata taaaagaagc
      781 ttgaaaaacc ggcatcatat gccgtatact gaaatggact gaaatataag gaatgaggag
      841 cgttttatat gccaaagata aaaagggaga caaaaacggc ccgcagccag cgggttgctc
      901 atatactgga agccttagac cgggaatacg gcacggagct gatctgttat ctgaaccatg
      961 aaacaccgtg gcagctgctg atcgccgtga ttttaagtgc ccagtgcaca gatgccaggg
     1021 taaatatcgt aacagccgac ctgtttcaga agtacccttc tctggaggcc tttgccgcag
     1081 cggatgtaaa agagatggag caggatatcc attccatcgg gttttaccat aataaggcaa
     1141 agaatatcat tctctgcgcc agagatttgc tggaacggtt ttcaggagag gtaccggagt
     1201 cgctggagga tctgacctcc cttgcaggcg tgggaaggaa aacagctaat gtgatccggg
     1261 gcaatgttta tcatgtgccg agcattgtgg tggataccca tgtaaagcgg atttcccgaa
     1321 agcttggcct gaccttggag gaggagccgg aaaaggtaga gtatgatctg atgaaggtgc
     1381 tgccaaagga ccactggatt ctgtggaata tccatattat tacactggga agaactgtct
     1441 gtacggccag aaaacccaaa tgcggagaat gctttttaaa ggaatactgc ccgtcctgcg
     1501 aagagccggt ggtttttgac gggggtaagg cgcgatgatg acttcttata ttgcccttga
     1561 tctggagacg actggcctgg aatcaaagac ggaaaaaatt acagaggcag cggccttaaa
     1621 ggtgattgac ggaacggtgc gggagcggtt tgtgacgctg atcaatcctg gaaggcctat
     1681 ttcagagaaa attacgcagc ttacgggaat tacagatgag atggtacgga cagcgcctct
     1741 catggaaaat gtgatcggag aacttctggc cttctgcgga ggtcttccgc ttttgggcca
     1801 caatatcctc tttgattacc gatttttaaa gcaggcagca gtaaacagcc agctggaatg
     1861 tgagtttgag gcggtcgata ccctgacgct gtgccgtcat ctgatgccgc cggatgagaa
     1921 aaagaattta tccgcttcct gtacctggtt tcagattccc cagtctgctg ctcatcgggc
     1981 agaggcggat gcagaatcag cccacctgct ttatgaaaag cttaaggccc tttatgggaa
     2041 ggaacgggag gagctttttg ttccatgtcc gctgatccac aaggcaaaac gggagcagcc
     2101 cgctacaaaa aggcaaaaag aatacttgca ggatttgata aaatgtcata gaatagacat
     2161 aactctgcag acggatactt taagcagaag cgaggcgtcc agactgattg atcagatcat
     2221 tctgcagcgg ggaagactta cagagcattc tctgtcttcc ccatataacg gttttgtggg
     2281 cggttccggc agaaggaagg gatcgcagga ttaaaagagg tgaaaaaatg ctgaacatga
     2341 gagaccataa gacaaagaag atcatttcaa ccattattgt tgtgatcctg gtactggcta
     2401 tgatcgtacc gatggcttta agtgcactga tgtattaaag gatctgtgat acgggccaaa
     2461 atgccggaaa aatggcttcc ggaatgaaaa ggatatgtga gaatgaaaaa taaaatgtgg
     2521 aaaacagccc tgactgtcct ggcggcagcc ggggcagtgt gggccttccc tggtacagcc
     2581 atggcggctc ctgtacttcc cgacgggatt cgtgtacagg ggcaggatct ggcaggaaaa
     2641 acctgtgggg aggcccgtga tgtaatagag acttatgagg aggaattggg aaactgtccg
     2701 gttctcctga ccctggacgg acaggagaag gagaccacgg caaaggagct gggactttac
     2761 tggagcaatg agaaccagat tgaggaaacg cttaaggagt acgccggagg cagcctgatc
     2821 cgccagtata tggtaaagaa agatcttgcc cgggctccgg tagagcttgc ggttgaaaca
     2881 gcagtagatc ctgagaaggt aacagctttt gtggataacc acagccagga tatcctgacg
     2941 cagcctcaga atgcctccat acgccgggaa aacggcgctt ttgtgattac agaggcagtt
     3001 tccggccgcg cggttgatac ggaagctacg gttcaggcgc tgaatgaggc gctggctaga
     3061 cagcaggagg gaacagtccg ggcggacgcg gtgatcacgg agcaggagcc ggagatcaca
     3121 tcggaggaac tggcgtccat ccaggatgtg ctgggcacct gtaccacaga cttttccagc
     3181 agcggagcag cccgttccac caatctttcc gtgggagcgg ccaagatcaa cggcagggta
     3241 atgatgcccg gagaagtgct ttctggctat gagtgcctgc agccctttac aacttccaat
     3301 ggatacaaga ctgcagccgc atatgaaaac ggcaaggtgg tagacagtat cggaggaggc
     3361 gtctgccaga tcgcgaccac gctgtacggc gcgtctctgg aagcagaggt ggaaattgtt
     3421 cagaggcaga accattccat gatcgttacc tatgtggagc cttccatgga tgcagccatt
     3481 gcgggaacct ataaggatat taaaattaaa aataattaca gtactcccat ctacgtagaa
     3541 gcctatactt ccgggaaaaa gctgaccttt accctctatg gaaaggaaac gcggccggag
     3601 ggacgaaagg tggagtatat ctcagaaacc ataggtacta ccagtcccgg tgaacctcag
     3661 ctgatcgttg acaatactct ggctcccgga gcgaaggttc gggtgcagtc ttcccatacc
     3721 ggactgcgat cccggttgtg gaaggtggtt acggtagacg gtgtggagac ggagcgcacg
     3781 ctgttaaata aagataccta taatgcttca aaggcagtct accgtgtggg tccggaagcg
     3841 ccggtcaatg cgcctgtgca gaccgatccc gcaggaggac agactgacgg agcaggggaa
     3901 aatacttctc ctgaatcacc cggtgcagaa aatgtggcag gtggccccgg aatgccggga
     3961 gaaagcggaa ctgtcccggg aggcggcccg ggagcaccag caggcggcac aagcggcagc
     4021 ggggcggcct caggcggtcc gggagcaccg gagggtgcag gaggaagcag cggagcagtt
     4081 tccggtggag gccccggagc atcgccagag gccggaggca gcgcagcagc tccgggaggt
     4141 ggcccgggag ttcccgccct atcaggggag gtggctcaat gagaacagca gggcaggggc
     4201 cgggcggctt tgaaaagcgg gacggccata taaatccggg acaggagctg atttttgcag
     4261 gttctgccgg tctggcagga gccagacgca tcctggaagc aaaaaaggaa atactttcca
     4321 ggcgatttgc tccctcattt ttaagagaac tgaaagaaga ggaatattcg gtaaaaagct
     4381 ggctggatca ggcgacagcg gaaaaaaaaa gtccggttac agcctgggag tttgcagggg
     4441 aaggcggcgt actggcagca ctctggaatt tttcaggcat ctactgcaca ggactggacg
     4501 tggatctgcg ccttttccct gtccatcagg cggcaatcga agtctgtgag ctgtttgaat
     4561 taaatcccta ccggctttac agtgaaaact gtgtgctcct tgcggcagac agcggcagcc
     4621 gcctggtgcg ggaattaaag caggcgggga ttccggcggc agtgatcgga tgggcggaaa
     4681 aaggcattgc aagaaagctc cgccacggtg aggaagcagc cggatatctg gagcgtcccc
     4741 agccggatga gcttttaaag ctgggactgt aatatatgcc ctcaggggat ttttttagca
     4801 gaatggagga tggttatgag agaaaaaatt cttgcggtga ttgaaaaaaa cagtaggatc
     4861 gatataaagg atctggcggt tttactgggc gaaagtgaga ttgcagtggc aaatgagatt
     4921 gcagatatgg aaaaagagca tattatctgc ggttatcata ccctgatcaa ctgggataat
     4981 accagtgagg aaaaggtagt tgcgctgatc gaggtaaagg taacgcctca gcggggcatg
     5041 ggctttgaca agatcgcaga gcgcatttac cagtacagcg aggtaaacgc tgtgtatctg
     5101 atgagcggtt cctttgattt taccgtattt atcgaaggaa agaccatgcg ccaggtagca
     5161 cagttcgttt ccgacaagct ggctcccatg gaggctgttt taagcacagc tacacatttt
     5221 gtactgaaaa aatataagga tcacggcact attatcagcg agccggtcca ggatgaaagg
     5281 atgctgatta ccccatgaga aatcccttat cagataagat cgtaagtatc cctccttctg
     5341 ggatccggaa attctttgat attgtaaacg agatggaggg agccatttct ctgggcgtag
     5401 gcgagcctga ttttgacaca ccctggcata tcagggagga gggcatctat tctctggaga
     5461 aaggacgtac tttttatacc tctaatgcag gccttaagtc cttaaaggac gagatcagcc
     5521 gttatttaag ccgccgtttt gatgtaagct atgattcaga caaagagatc atggttacgg
     5581 tgggcggcag tgaagccatt gatgcagcca tgcgtgccat gttgaatccc ggggatgaag
     5641 tcctgattcc ccagcccagc tatgtttcct atgtgccctg tgccgtgctg gcggacggtg
     5701 tgccggtggt gattgaactg gaggaaaagg atcattttaa actgacccct gagaaacttc
     5761 tggaaaagat taccgataaa acgaaaatcc tggttcttcc atttccaaat aatcccactg
     5821 gagcgatcat ggagcgtcat gagctggaag agattgctaa gattgtggtg gaaaaggata
     5881 tttttgtaat ttcagacgaa atctattccg agcttacata tacagataat cacgttacca
     5941 tcgcttcctt ccccggaatg aaggaaagaa cggtattgat caacggcttt tccaaatcct
     6001 atgccatgac aggctggcgt ctgggatatg cggcggctcc ggagattatt ttaaagcaga
     6061 tgttaaagat ccatcagtat gccatcatgt gtgctcccac taccagccag tatgcggcgg
     6121 tcgctgccct tcgggacggg gataatgatg tggaaatgat gcgtgaatct tataaccagc
     6181 gccgccggta cctcttaaat gcatttaagg aaatggggat cgactgtttt gagccgttgg
     6241 gcgcctttta caccttcccc aatatcagcc gatttggtat gacctctgag gaatttgcca
     6301 cagcgctgtt aaaagaggaa aaggtagctg tggtgccggg aacggccttt ggagcgtgcg
     6361 gagaagggtt tgtgcgtatt tcctatgcgt actccctaaa gagcttaaaa gaggccttag
     6421 gccgcattga gcgctttgta aagaaccatg acggaaaggc gtgacagcta tgatgaatat
     6481 tgtactttta gaaccggaaa tgccttctaa tacaggcaac atcggccgca c
//
LOCUS       DBMDUH010000149         6508 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_4915_length_6508_cov_5.208366, whole genome shotgun sequence.
ACCESSION   DBMDUH010000149 DBMDUH010000000
VERSION     DBMDUH010000149.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 6508)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6508)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6508
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4915_length_6508_cov_5.208366"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="ACZPCG_11025"
     CDS             complement(<1..971)
                     /locus_tag="ACZPCG_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HIR1285623.1"
                     /translation="MSAASIKDFLKRKNVSITVQTYLIDALGAMAFGLFASLLIGTIF
                     GTLGQQFHIEIFNVIADYAKSATGAALGIAIAHALKAPQLVLFSAATVGIAGNALGGP
                     VGALVATIAAAELGKIVSKETRLDILVTPGVTIISGVLVAQFVGPGVAGFMSWFGNLV
                     KTATEMQPFIMGILVSALIGVALTLPISSAAICIALSLDGLAGGAATAGCCAQMIGFA
                     VMSYRENKIGGLMAQGLGTSMLQMGNIVLNPRIWIPPTLASMITGPIATMVFHLENIP
                     AGSGMGTCGLVGPIGIYTAMGGGTSMWLGILFVCFLLPAVLTFIFGEA"
     gene            complement(1192..1929)
                     /locus_tag="ACZPCG_11030"
     CDS             complement(1192..1929)
                     /locus_tag="ACZPCG_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285624.1"
                     /translation="MKKRFITTAAMAAITATMLLNGCGSSQTASTSAPQTETQAPSTE
                     AKPDETEETTAAPETGGSETEALAEDVVRTEAVYVKDSEFAEDGRLVFHNDTHGDFIC
                     NISDFTVLPEELKDGETYVISHSMVMTMSLPGQLPQVYSIRTADDTYQDSIGTVQAIE
                     EENVLFEQADGNQFMISKDSVDDIEKLQEGDVCVVSHTEAMTRSMPGIYMEVNRVDVL
                     PQADSETKEASAETLTEIKEAETTKAE"
     gene            2155..3942
                     /gene="pepF"
                     /locus_tag="ACZPCG_11035"
     CDS             2155..3942
                     /gene="pepF"
                     /locus_tag="ACZPCG_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002593498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HIR1285625.1"
                     /translation="MKKRDEIPAEATWKLEDMVENREAWEKLYEEAVRETDRYQEFKG
                     TLGRSADRLFEGLSFDDSISQKIERLYVYARMRSDEDTAGQQAQDMFSRAQNLSFRAG
                     ELSSYMVPEILSIPDEQLEAFQREKKEFGLYGRLLSQLLKRKAHTLSDELEALLAASQ
                     EATQGGAQVFRMFNNADVRFPSITGEDGRELPVTHGTYISLMEHPDRRIRREAFQSLY
                     SVYRQYSNTLAAAFGANVKQAVFYAKARKYTSSREYYLSSNEVPEGVYDRLVSSVRKS
                     LPLMHRYMSLRKRLLGVDELHMYDVYVSLTSQGNTHYTFEEAKKIVKEGLAPLGEEYL
                     SLLETGFEGRWIDVYENEGKRSGAYSWGIYGCHPYVLLNFHGTLNDVFTLAHEMGHAI
                     HTWYSNENQPYVYAGYKIFVAEVASTCNEALLIRYLLKQCKEKKERAYLLNHFLESFR
                     ATLFRQTMFAEFEKTVHELGAAGESLTSEKLCGIYQKLNEDYFGPDMVSDSEIGYEWE
                     RIPHFYTPFYVYQYATGFSAAVAISRRIMEGEPGILEGYKRFLSGGCSMEPVELLKLC
                     GVDMSESRPVDQAMEFFEELLDEFEVCMK"
     gene            4005..4694
                     /locus_tag="ACZPCG_11040"
     CDS             4005..4694
                     /locus_tag="ACZPCG_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008399178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HIR1285626.1"
                     /translation="MLGTIANTGAILTGCVVGSTLKKGIGEKYKTVMMDSMGLAATAL
                     GINSIAEAMPESQYHVLFIVSLALGGLIGTGLDLEAVFDRAVGRISGSSELSKGLSTA
                     ILLFCAGTLSILGPMQSALLGDNTFLYTNAVLDGITSTVLSSAFGFGIAIAAAVLFCW
                     QGSIYLLASVIEPFITDALMTEISLVGGILILSSGLGILGIKQIRTLNLLPALLVPPV
                     VIALLIAVGMG"
     gene            4728..>6508
                     /locus_tag="ACZPCG_11045"
     CDS             4728..>6508
                     /locus_tag="ACZPCG_11045"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002605184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HIR1285627.1"
                     /translation="MNWNFYFSGILYAKIREETEALTVRILDIETGNLGEERPFGEKE
                     NVFLQKLCSRSIAGFFEKKAACEEKIAQEPEVFTLDGERFTNRRRESYIQRDKKFPMD
                     LFYENGKIWAVLMSGRDYTGILVKEGMEENTVLKIWDEVYPDRQCFGVRFLGTEFVKT
                     RDGEMLASDVYLPSEGQRFPTVLIRTPYGKSRGLEGYYRFVQRGYAVVIQDVRGREDS
                     TGKWMPQYYEIEDGDDALNWIAAQSWSDGSVGMTGGSYLGYVQWAAAASGNPHLKAML
                     SSVCAGSAFVDLPRRGGCFDSGMLAWCFSVSEQRMRPDLMEQENWDEILDIRPIEDIP
                     KKALGRELPFLTEWLRHKDMDEFWKKGNWHLNYKGGPVPALIMSGWFDDNGMGTSEAL
                     ELVKDWPEGTWKAVLGPWKHSGNADYDLHGIHMGPDALRYDMDILCMKWLEHFLKGIE
                     NGIERTPALEYYTLGDNTWKNGQCWPPANTEEAILYLNGAEDDSAAGNGAACGHGSLS
                     FRAPETGGRDSYLYDPGNPSVHIIDMSENELEVPEDYTVIEQREDMLSYTTEPFKESF
                     TITGELAVKLYVSCDCPDTDFMVRITDV"
BASE COUNT         1673 a         1328 c         1816 g         1691 t
ORIGIN      
        1 gcttctccga aaataaaggt caatacagcg gggaggagga agcagacgaa aaggattccc
       61 agccacatac tggttccgcc tcccatggca gtgtagattc cgatggggcc taccaggccg
      121 caggttccca ttcctgaacc ggcaggtata ttttccagat ggaataccat agtggcgata
      181 gggcctgtga tcatggatgc aagtgtgggg ggaatccaga tccggggatt taaaacaata
      241 tttcccattt gaagcatgct ggttcccagc ccctgagcca tcaggccgcc gattttattt
      301 tcccggtaac tcataacggc gaagccgatc atctgtgcgc agcagcctgc ggtagcggcg
      361 cccccggcca gaccatccag agaaagggca atacagatgg ctgcgctgct gatgggaagg
      421 gttaacgcga cgccgatgag ggcagaaacg aggattccca tgataaaggg ctgcatttct
      481 gtagcagttt ttacaagatt gccgaaccag ctcataaagc ctgccacccc cggtcctacg
      541 aactgtgcta ccagcacgcc ggagatgata gtaactcccg gagtgaccag aatatccaga
      601 cgggtttctt ttgacacgat ttttcccagc tctgcggctg cgatagtagc taccagagcg
      661 cctacaggcc ctcccagggc gtttccggca atacctacgg ttgcggcgga aaagaggaca
      721 agctggggag cttttaaggc atgggcaatg gcaataccca gagccgcgcc ggtagcgctt
      781 ttggcatagt ctgcgattac attaaaaatt tcaatgtgga actgctgtcc aagagttccg
      841 aaaatggtgc cgatcagaag ggaagcaaaa aggccgaagg ccatggctcc caaggcatca
      901 atcagatagg tctggacggt gatgctgaca tttttccgtt taaggaaatc cttgatactg
      961 gctgctgaca taatagattc tccctttttt ctgctgccgg agccaaaatc acagggaaat
     1021 cctgcagatg tcggttccag gcaaggtttg ctagggattt tatcacatat ttacgaaatt
     1081 gcaaggattt tgaagaattt cacaagatat tgttaagaaa ataacaaaaa aatacctccc
     1141 tgaatgacag gcaaaacagc tctgtgaatc agggaggtat ttatataaga attactctgc
     1201 ctttgttgtt tctgcttcct ttatttctgt aagggtttcg gcagaagctt cttttgtctc
     1261 agaatctgcc tggggaagta cgtctacgcg gtttacctcc atataaattc cgggcatgga
     1321 acgggtcatg gcttctgtgt ggcttactac acatacgtcg ccttcctgaa gcttctcaat
     1381 gtcgtccaca ctgtctttgc tgatcataaa ctgatttccg tcagcctgct caaagagcac
     1441 attttcttct tcgatggcct gtacggtacc gatggaatcc tgataggtat catctgcagt
     1501 gcggatggaa tatacctgag gaagctgtcc gggaagggac atggtcataa ccatggaatg
     1561 gctgatcaca taggtttctc cgtcctttaa ttcttccggt aaaacagtga agtcgctgat
     1621 attgcagata aaatctccgt gggtatcatt gtggaagacc agcctgccgt cctctgcaaa
     1681 ttcactgtct tttacataaa cagcttctgt gcggactacg tcctcggcca gagcctctgt
     1741 ttctgatccg ccggtttccg gcgctgcagt agtttcttca gtctcatcag gtttggcttc
     1801 tgtagaagga gcctgtgttt ctgtctgagg agcagatgta gaggctgtct gggaactgcc
     1861 gcatccgttt aataacatgg ttgctgtgat agctgccata gctgctgtgg tgataaatct
     1921 ctttttcata attaaaatct ccttttgagt ttgttttctt tttacatgta tgattatagc
     1981 ggatagatat tacaaaaacg atcactttct tttgactatt ttataataat ttctgacttt
     2041 tttgttaagg aggaggggaa atattaaaaa atgataggga aatctggttt acaacccctt
     2101 ccaatgtgat aaaataagga atgattttgc aatggaagga gccttgggct tatgataaaa
     2161 aagagagatg aaataccggc ggaagccacc tggaagctgg aggatatggt agagaatagg
     2221 gaagcgtggg agaagctgta tgaagaagcg gtccgggaaa cagaccgcta tcaggagttt
     2281 aagggaacac tgggacggtc ggcggatcgg ctttttgagg ggctttcatt tgatgattct
     2341 atttcacaga aaatagaacg gctgtatgta tatgccagaa tgcgttccga tgaggacacg
     2401 gcgggacagc aggcccagga tatgttttcc agagcccaga acctgtcttt tcgggcagga
     2461 gagctgtcgt cctatatggt gccggagatt ctttcaatcc cggatgaaca gctggaagcg
     2521 tttcagaggg agaaaaagga gtttgggctg tatggcaggc tgttaagcca gctgcttaag
     2581 agaaaggccc atacgctttc cgatgagctg gaggcgcttc tggctgcttc tcaggaggcc
     2641 acgcaggggg gagcacaggt attccgtatg tttaataatg cagatgtgcg ctttccatcc
     2701 attaccgggg aggatgggag agagcttcct gtaacccatg gaacctatat ttccctgatg
     2761 gagcatccag accgcaggat acgcagggag gcatttcaaa gtttgtatag tgtatacaga
     2821 caatattcaa acaccctggc agctgctttt ggcgcaaatg tgaagcaggc tgtattttat
     2881 gccaaagcac gaaagtatac ctccagccgg gaatattatc tgagttcgaa tgaggtgcct
     2941 gaaggggtgt atgaccgtct ggtgtcatcg gtgaggaaga gccttcctct tatgcaccgc
     3001 tacatgtccc tgagaaagag gcttcttggg gtggatgagc ttcatatgta tgacgtttat
     3061 gtttctctta catcgcaggg gaatacacat tatacctttg aagaggcaaa gaaaatagtg
     3121 aaggagggac tggctccact gggggaggaa tatctgtccc tgcttgagac aggctttgag
     3181 ggccgctgga ttgatgtata tgaaaatgaa ggaaagagaa gcggagctta ttcctggggg
     3241 atttacggct gccatcccta tgtgctgtta aatttccacg gcactttaaa tgatgtattt
     3301 acactggctc acgagatggg gcacgccatt catacctggt attccaatga aaaccagccc
     3361 tatgtctatg caggctataa gatttttgtg gcagaggtag cgtctacctg caatgaggcc
     3421 cttctgatcc gctatctgct gaagcagtgt aaagaaaaga aggaacgggc gtatttgtta
     3481 aaccattttc tggaaagctt ccgggcaact ctgttccgtc agaccatgtt tgcggaattt
     3541 gaaaagacag ttcatgagct gggagcagcg ggagagagct tgacttctga aaagctgtgc
     3601 ggaatttacc agaaattaaa tgaggattat tttgggccgg atatggtttc tgattcggaa
     3661 attggttatg aatgggaaag gattccccat ttttatacac ctttttatgt atatcagtac
     3721 gcaaccggtt tttcggctgc cgttgccatc agccgccgga tcatggaagg ggagccgggg
     3781 attttagagg gctataagcg gtttttaagc ggaggctgca gtatggagcc ggtggagctt
     3841 ttaaagctgt gcggcgtgga tatgtctgag agcaggccgg tggatcaggc aatggagttc
     3901 tttgaagagc ttctggatga atttgaggta tgcatgaaat gacaatggac cggacggaag
     3961 gtgatttaaa gtaagggtaa atttggaaac aggaggaatg tattatgttg ggaaccattg
     4021 caaatactgg cgcaattctc acgggctgcg tagtgggaag caccttaaag aagggaatcg
     4081 gagaaaagta taaaactgtg atgatggatt ccatggggct ggcagccaca gcccttggaa
     4141 tcaattccat tgcagaagcc atgcctgaat cacagtatca tgttctgttt attgtaagtc
     4201 tggccctggg cggcctgatc ggtaccgggc ttgatctgga ggctgtcttt gaccgggctg
     4261 tgggaaggat ttccggcagc tctgagctgt ctaagggact gtccacagcg atcctgctgt
     4321 tctgtgcagg cacgctttcg attctgggac ccatgcagag cgcccttctg ggagataata
     4381 ccttccttta taccaatgct gttctggacg gaattacttc tacggtactc agctctgcct
     4441 ttggcttcgg tatcgccatt gcggcagcgg tgcttttttg ctggcagggg agtatctatc
     4501 tgctggcaag tgtgattgaa ccctttatta cagacgctct gatgacggaa atcagtctgg
     4561 taggcggaat tttaatttta agcagcggtc tgggcatact gggaatcaag cagatccgta
     4621 cattgaatct gctcccggcc cttttggtgc cgcctgtggt gattgccctt ctgatagcag
     4681 tggggatggg atgaggagta catatgccga ttggaggata ataaaatatg aactggaact
     4741 tttacttttc cggtatttta tatgcaaaga tacgggaaga aacggaagct ctgacggtcc
     4801 ggattctgga tattgaaaca ggaaatctgg gcgaagaaag acctttcgga gaaaaggaaa
     4861 atgtattttt acagaagctg tgcagccgca gcatcgcagg cttttttgaa aagaaagcag
     4921 cctgtgagga gaaaattgca caggagccgg aagtctttac cttggacggg gaacggttta
     4981 caaaccgtcg gagagagtcc tatattcaga gagataagaa atttccaatg gacttatttt
     5041 atgaaaatgg aaaaatctgg gcagttttaa tgagtggacg cgattatacc ggcattctgg
     5101 taaaagaggg tatggaagag aatacggttc tgaaaatctg ggatgaggta tatcctgaca
     5161 ggcagtgttt tggtgtccgg tttctgggaa cagagtttgt aaagaccagg gacggggaaa
     5221 tgctggcttc ggatgtatat cttccctcag agggacagcg ttttcccacg gttctgatcc
     5281 gtacccctta tggtaaatca agagggttgg aaggatatta ccgctttgtc cagagaggct
     5341 atgcagtggt gattcaggat gtaagaggac gggaggacag tacagggaaa tggatgcccc
     5401 agtattatga aattgaagat ggagatgatg ccttaaactg gattgctgcc cagtcctgga
     5461 gcgacgggag tgtgggaatg accggaggct cctatctggg atatgttcag tgggctgctg
     5521 ctgccagcgg aaatcctcat ctgaaggcta tgctttccag tgtgtgcgcc ggaagcgcct
     5581 ttgtagacct tccaagaagg ggcggctgct ttgactcagg tatgctggca tggtgttttt
     5641 ctgtgtcaga acagaggatg cgtccggatc ttatggaaca ggagaactgg gatgagattc
     5701 tggatatccg tcccattgag gatattccga aaaaggctct ggggcgggag ttgccctttc
     5761 tgacagaatg gctgaggcat aaggatatgg acgaattctg gaaaaaggga aactggcatc
     5821 tcaactataa gggcggaccg gttcctgcgc tgatcatgtc cggatggttt gatgataacg
     5881 gaatgggaac ctcagaggct ctggagctgg taaaggactg gcctgaggga acctggaagg
     5941 cagtgctcgg tccctggaag cacagcggca atgcagacta tgatctccac ggtattcata
     6001 tggggccgga tgccctgcgc tatgatatgg atatcctctg tatgaagtgg ctggagcatt
     6061 ttttaaaggg aatagagaat ggcattgaga ggacgcctgc gctggaatac tatactctgg
     6121 gtgataatac ctggaaaaac gggcagtgct ggccgccggc caatacagaa gaagcaatcc
     6181 tttacctgaa cggagcagag gacgattcag ctgccggaaa tggagccgcc tgcggtcatg
     6241 gaagcctgag ctttagggct cctgaaacag gtggaaggga ttcctatctt tatgatcccg
     6301 gaaatccgtc cgtacatatt atagatatgt ctgaaaatga actggaggtt ccggaggact
     6361 atacggtgat cgaacagcgg gaggatatgc tttcctatac cacagaaccc tttaaggagt
     6421 cctttacgat tacaggcgaa ctggcagtga agctgtatgt ttcctgtgac tgtcctgata
     6481 cagattttat ggtccgaatt acagatgt
//
LOCUS       DBMDUH010000150         6485 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_4931_length_6485_cov_4.498120, whole genome shotgun sequence.
ACCESSION   DBMDUH010000150 DBMDUH010000000
VERSION     DBMDUH010000150.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 6485)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6485)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6485
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4931_length_6485_cov_4.498120"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(99..3476)
                     /locus_tag="ACZPCG_11050"
     CDS             complement(99..3476)
                     /locus_tag="ACZPCG_11050"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR1285628.1"
                     /translation="MDIREDLNIYMQIIRLLDESVNGYLYACDLQDDRMYFTDKICRR
                     FALPFSGEQGIPFKEWEAVIYDRDVKLIKDDMEAVRSGKRNGCDIECRMIDREGNRVG
                     VHWRATVHADKSGKPAVLLGSMDELVLGQRIDSLTGLWNYDKCTEDLESAVLKEEGYF
                     IVFGIDNFKHINVKNGRPFGNDVLKRMTESLEKNADCSVTLYRLDGDCFAATLPGKGK
                     KEVFSFYDTVRQEMEKECTLSAGAAAYSPNRGMDGWTVYQFAENALDRAKKEGKNMLI
                     FFSPEDYQKKLDQIELLDELKSAVDQGCRGFYLCYQPQIDRKNYGLYGAEALLRYESG
                     KRGMVGPDQFIPLLEQSGLICEVGLWVLRTAIRQSVLWRRILPSFHISVNISYVQLRQ
                     KEIEEEVLGILREEGLPGDALTLEVTESMQLQDYTYYNKIFYDWKRKGIQIAIDDFGT
                     GYSSLSYLKSIDIDETKIDRCFVNRIQKNAYNYRLLDNVIQLAHSARIRVCCEGVETE
                     EELFVLKELCPDVLQGYLFARPCRKEEFEQTYLNRDSKAYKERRERLLKFRCPVARDG
                     KLDGTQERTEETYEDILEHTSLGLWRICADFETECYELYADPVMLRVMGVETALSPKE
                     CYFHWYNRINDGYYNYINLAIQSAVETGKITQVEYTWNHPSRGEVTVRCMMIRAKREN
                     STVWLEGYHRIISDLERPDFLPGGLKSEIFEYNEHKKTIYFHTKRQMIAGDKIREEDF
                     PECWIRKQIVHPHFADEFQTMFRDVKDKWNRAGSEMLFRTKSGSYEWFKVKTRHLSDK
                     KEDVYTVAVLMDPADQERAIELEYMKKTDFYEALLSETVAHAEIDVESGHIMEAAGLW
                     AFVQSKDQEQDFESIVQQQMRSDQIFCEDRELYESYMDLSYMKEMYKSGTNTVELCFR
                     HYVKERLCWMKLVIHVFQDHYTENMYALVYLKNIDAEKRRELAAETAAKSDPLTRVYN
                     RSSFEEEVSDFMTTGEDSARGALLILDLDDFKQVNDRFGHLKGDEVLKSLAEVLRHTF
                     RSHDLIGRLGGDEFLVFVKGIENKEILDRRMDQLFSRLNDSCGDFLFCSAGICFVSGE
                     GFDYQKALKCADTALYESKKKGKRRYSYYEKMEDMKV"
     gene            complement(3518..4624)
                     /locus_tag="ACZPCG_11055"
     CDS             complement(3518..4624)
                     /locus_tag="ACZPCG_11055"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024126.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR1285629.1"
                     /translation="MSNTGERILPVSRYYDSLNGMKRKYDRLARQEAFSGTNVEEWTG
                     WKNKEKKRLWELLGMDKMEICSPCPEIAERVMLDGGILREKLLLQVEPDIWMPVYLLI
                     PPGSGRHSDCVLAPHGHQGAGKYSVAGCSEIPAVAEAIDRFHYDYGLYLARLGYVAVC
                     PDARGFGERRDLAFQKDEENAFMRGTCFYLAHMAEPLGMTVAGMNTWDLMRLIDYLEE
                     RGEWNTEHLGCVGFSGGGLQTLYLAALDERVRYAVISGYLYGFKDSLLELNGNCSCNY
                     VPGLWNALDMGDIASLIAPRPLVIQSGRADHLNGPRGIVNAEEQVEIVRKAYGLNGAA
                     ECLIHRCYEGGHQFHEEDLEEDLKRMKFFTQISG"
     gene            complement(4617..5267)
                     /locus_tag="ACZPCG_11060"
     CDS             complement(4617..5267)
                     /locus_tag="ACZPCG_11060"
                     /inference="COORDINATES: protein motif:HMM:NF016726.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HIR1285630.1"
                     /translation="MLRYDGPFITFLMKLGDMIILHVLWLLCSLPVITAGPALAAAHY
                     AALRIVRDEGGSVCSMFFKAFRINFFQALPLGVISLLAGAVIGTDLYLLSGPVQPKGA
                     AGLFMLALTVFLSILYGILMLWLWPVLVTFENTWMGTIKSAYMMAASNWGATSAMILQ
                     DLVLGIAAVFSIAFLPQAAIIFLIFGCPLFFVVNAFHIRRAFDRIRENSSKEDEDE"
     gene            complement(5274..>6485)
                     /locus_tag="ACZPCG_11065"
     CDS             complement(5274..>6485)
                     /locus_tag="ACZPCG_11065"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1285631.1"
                     /translation="AVVAFRAKIHRENRSFRQGMEETSARVMEMVEMVPVARAHALED
                     KEAARISALLEETANRGYRLDMVQAHFGAVSWVVFQVFQVVCLAVSGFMAMSGLIKVG
                     DVTFYQSSFTTVVNQFSALINLLPILTKGLESVSSIGEVLSSNEIEENEGKEELLSLR
                     GEYAFKQVSFSYPGSSKEVLHGLDFTVKQGETIALVGESGSGKTTILNMLIGFILPSG
                     GQLMLDGKDMKGLNLRTYRRFLSVVPQTPVLFTGTVRENITYGLEHVSEEQIAQAVEA
                     ANLSEVVKRLPQGLDTMIEEHGANLSGGQRQRISIARALIRNPQVIILDEATSALDSI
                     SESEIQEALERLTKGRTTFIVAHRLSTVRGADRILVIGQGRIREQGSYQELMAKKGEF
                     YQMEQLQRMVR"
BASE COUNT         1635 a         1836 c         1252 g         1762 t
ORIGIN      
        1 cgatcatcaa gaaaaacgaa aaagctgccg gctgaaaaaa aatacctgaa aaaagccctg
       61 tgcatccgtt catccggatc tcacagggct ttttcctttc agactttcat atcttccatt
      121 ttttcgtaat agctgtacct tctttttccc ttcttcttgc tctcatacag ggccgtatct
      181 gcgcatttga gcgctttttg ataatcaaag ccttctcccg atacaaaaca gataccggca
      241 ctgcagaaaa gaaagtcccc gcaggaatca ttcagccgac taaacagctg atccattctc
      301 ctatccagga tttccttatt ttcaatgcct tttacaaata caagaaattc atctccgccc
      361 agacgtccaa tcagatcgtg gcttcggaag gtatggcgca gcacttctgc cagtgatttc
      421 aatacctcat ctccctttaa atggccaaag cggtcattga cctgtttaaa atcatctaaa
      481 tccaggatca gcagcgctcc ccttgcagaa tcttccccgg ttgtcataaa atcagatacc
      541 tcttcttcaa agctgctccg gttgtatacc ctggtaagag gatcactttt ggccgctgtc
      601 tcagccgcaa gctctctcct cttttcagca tcaatatttt tcagatacac aagagcatac
      661 atattttctg tataatgatc ctgaaatacg tggatcacca gcttcatcca gcagagacgc
      721 tctttcacat agtggcggaa gcacaattca accgtattgg ttccgctttt atacatttcc
      781 ttcatatagg aaagatccat atacgattca taaagctccc gatcctcaca aaaaatctga
      841 tcacttctca tctgctgctg aacaatgctt tcaaaatcct gttcctgatc cttgctctgg
      901 acgaatgccc acagccctgc tgcctccata atatggccgc tttccacatc tatctctgca
      961 tgggccacag tttccgaaag aagagcctca taaaaatccg tctttttcat atattccagc
     1021 tctatggcac gttcctgatc tgcaggatcc atgagcactg caactgtata aacatcctcc
     1081 tttttatcgc tgaggtgacg ggtctttacc ttaaaccact cataggaacc ggatttggtc
     1141 cgaaaaagca tctcgctgcc tgcccgattc cacttatcct taacatcccg gaacatagtt
     1201 tgaaattcat ctgcaaaatg aggatggaca atctgttttc ggatccagca ttccggaaaa
     1261 tcctcttccc ggatcttatc tcccgcgatc atctgacgct tcgtatggaa ataaatggtt
     1321 ttcttatgct cgttgtattc aaagatctcg ctcttaagac cgccaggcag aaaatcaggc
     1381 cgttccagat cgctgatgat ccggtgatac ccctccagcc agaccgtgct gttctccctt
     1441 ttggcccgta tcatcataca gcgtaccgta acctcacctc tggaaggatg attccatgta
     1501 tactcgacct gtgtaatctt acccgtttca accgcactct ggatcgcaag gttaatatag
     1561 ttgtaatatc cgtcattgat cctgttgtac cagtggaaat aacattcttt tggcgaaaga
     1621 gccgtttcca ctcccatgac ccgaagcatc accggatctg catacagctc gtagcactct
     1681 gtttcaaaat ctgcgcatat cctccacagt cccaggctgg tatgctccag aatgtcttca
     1741 tacgtctcct ccgtacgctc ttgtgtacca tccagctttc cgtctctggc aaccggacac
     1801 ctgaatttta acagccgctc tctccgttcc ttataggcct ttgaatcccg gttcagatag
     1861 gtctgttcaa attcctcttt tctgcagggt ctggcaaaaa gatatccctg aagcacatcc
     1921 ggacacagtt cctttaaaac aaacagctct tcttccgtct caacgccttc acagcagaca
     1981 cgtatccggg cgctgtgagc cagctgtatt acattatcca aaagcctgta gttgtaggca
     2041 tttttctgga tacgattcac aaaacagcgg tcaatctttg tttcatcaat atcaatgctt
     2101 tttaaatagc ttaagctgga gtatcctgtt ccgaaatcat caatggcaat ctggattcct
     2161 tttcgcttcc agtcataaaa gatcttatta taataggtat agtcctgaag ctgcatactt
     2221 tccgtcactt ccagagtgag agcgtctccc ggcagtcctt cttccctgag aatacctaaa
     2281 acctcttcct cgatttcctt ttgacgaagc tgcacatagg atatatttac gctgatatga
     2341 aagcttggaa gaatcctacg ccataatacg ctctgtctga tggctgtcct cagtacccat
     2401 aatcctactt cacagatcag gccgctctgc tccagcagag gaataaactg atcgggccct
     2461 accatcccgc gttttcctga ttcatagcga agcagcgctt cggctccgta caatccgtag
     2521 ttttttctgt caatctgggg ctgataacag agataaaatc ccctgcagcc ctgatcaaca
     2581 gctgacttaa gctcatccaa aagctcaatc tgatccagtt ttttctgata atcttcggga
     2641 gagaagaaaa taagcatatt ttttccctct ttttttgccc ggtcaagggc attttccgca
     2701 aactggtata cggtccagcc atccatgcct cggttagggg aataagcggc agctcccgct
     2761 gataaagtgc actctttttc catctcctgc ctgacggtat cgtaaaagga gaatacttcc
     2821 tttttccctt ttcccggaag ggttgctgca aaacagtctc catccagccg atacagcgta
     2881 accgaacagt ctgcattttt ttccagactt tctgtcatcc gctttaaaac atcatttcca
     2941 aagggtcttc cattttttac attgatatgc ttaaaattat caattccaaa aacgataaaa
     3001 tatccctctt cttttaaaac agcagattcc agatcctctg tacacttatc ataattccac
     3061 aatccggtca gactgtcgat cctctgtccc aggacaagct catccatgct ccctaaaagg
     3121 actgccggct ttcctgactt atccgcatgc accgttgcac gccaatgaac gcccacccgg
     3181 tttccttccc tgtcgatcat ccggcattcg atatcacagc cgtttctttt acccgaacgg
     3241 accgcctcca tatcgtcttt tatgagcttt acatcccggt cataaataac agcttcccat
     3301 tctttaaacg ggattccctg ctctccggaa aaaggcagtg caaatctgcg gcagatctta
     3361 tctgtaaaat acatgcggtc atcctgcaga tcacaggcat aaagatatcc attgacgctt
     3421 tcatccagaa gacggatgat ctgcatatat atattcaaat cttcccttat atccatatgt
     3481 atcaaatctc ctttattgat actatgtatt ttatcatcta accggagatt tgtgtaaaaa
     3541 atttcattct tttcagatct tcttccaggt cctcttcatg gaactgatgg cccccttcat
     3601 aacagcggtg gatcagacat tctgcggcgc cgtttaaccc ataggctttt cgtacgatct
     3661 ccacctgttc ctctgcattg acaatgcccc ttgggccgtt taagtgatct gccctgccgg
     3721 actggatcac aagtggtctg ggcgcaatga gggacgcaat atctcccata tccagcgcat
     3781 tccagagccc cgggacataa ttgcagctgc aatttccatt taattccaga agagagtctt
     3841 taaatccata cagataaccg ctgatcactg cataacgtac acgctcatcc aatgcagcca
     3901 gatacagcgt ctgaagtcct ccgccagaaa agcccacgca gcccagatgc tctgtattcc
     3961 attctcccct ctcttccaga tagtcgatca ggcgcatcag atcccaggta ttcattcctg
     4021 ccacagtcat tccaaggggc tctgccatat gggccagata gaaacaggtt ccgcgcataa
     4081 atgcattttc ctcatccttc tgaaaagcca gatccctccg ctcgccaaag cctctggcat
     4141 cgggacagac ggcaacatat cccagccggg ccagatagag gccgtaatcg taatggaaac
     4201 ggtcaatcgc ctctgccact gccggaatct cactgcagcc ggccacactg tattttcccg
     4261 ccccctgatg tccatgggga gcaaggacac aatcggaatg acgtccgctg cccggcggga
     4321 tcagcagata caccggcatc catatatccg gttccacctg caaaagaagc ttttccctca
     4381 ggattcctcc gtccagcatg actcgttccg ctatttcagg gcagggggag cagatttcca
     4441 ttttgtccat tcccaaaagc tcccacaagc gtttcttttc cttatttttc catcccgtcc
     4501 attcttccac gttcgttcct gaaaaagcct cctgccttgc cagacggtca tattttcgct
     4561 tcatcccgtt taggctgtca taatagcggc tcactggcag gatccgttct cctgtgttac
     4621 tcatcttcgt cctccttact gctattttcc cttatcctgt caaaggctct gcgtatatgg
     4681 aaggcattga ccacaaaaaa cagcggacag ccgaaaataa gaaaaataat ggccgcctgg
     4741 ggcagaaagg caatactgaa tactgcagcg atccccagta caagatcctg aaggatcatg
     4801 gcagaggttg ccccccagtt tgatgctgcc atcatgtatg cactttttat ggttcccatc
     4861 catgtatttt caaaggttac aagtaccggc cacagccaca gcataagaat cccgtaaaga
     4921 atggaaagaa acacggtaag agccagcata aaaagtcccg ccgctccctt tggctgtacc
     4981 gggccggaaa gcaggtacag atcggttccg atcaccgcac cggctaaaag acttataact
     5041 cccagaggaa gcgcctgaaa aaaattaatc cggaaggctt taaaaaacat ggagcacaca
     5101 gagccgcctt catccctgac aatccgcagg gctgcataat gggcggctgc caaagcaggt
     5161 cctgccgtta taaccggcag gctgcagagg agccagagta cgtgaaggat gatcatgtct
     5221 cccagtttca ttaaaaacgt tataaatggt ccgtcatatc gcagcatatt ttcctacctc
     5281 accattctct gaagctgttc catctgataa aattctcctt tttttgccat cagctcctga
     5341 tagcttccct gttccctgat ccttccctgt ccgatgacaa ggatcctgtc ggctccgcgc
     5401 acggtagaaa ggcggtgagc cacaataaag gtagtccttc cctttgtgag gcgttctaag
     5461 gcctcctgga tctccgactc agagatgctg tccagagccg acgtagcctc atccagaata
     5521 atgacctgag gattgcggat cagcgccctt gcaatggaaa tcctctggcg ctggcccccg
     5581 cttaaattgg ctccatgctc ttcgatcatg gtgtcaagcc cctggggcag cctttttacc
     5641 acctcggaca gattggctgc ttctaccgcc tgcgcgatct gttcctcgct cacatgttcc
     5701 agaccatagg taatattttc ccgcaccgtt ccggtaaaca gtaccggcgt ttggggcacc
     5761 acggacagaa aacggcggta ggttcttaaa ttcaggccct tcatgtcttt cccgtctaac
     5821 ataagctgtc ccccagaagg caggataaaa ccaatgagca tatttaaaat agttgtcttt
     5881 ccagagcccg actcgcctac caaagcaatg gtttcaccct gttttaccgt aaaatccaat
     5941 ccgtggagca cttctttgct gctgcccgga taggaaaaac ttacctgttt aaaagcgtat
     6001 tctcctctta gggaaagcag ctcttccttt ccttcatttt cctctatctc attcgaggac
     6061 agtacctccc cgatggagga aacggattcc aatccctttg taagaatagg aagcagattg
     6121 atcagagcag aaaactgatt cactaccgtc gtaaagctgg actgataaaa ggtcacatcc
     6181 cccaccttta tgagaccaga catagccata aaaccagaaa ccgccagaca taccacctga
     6241 aatacctgaa ataccaccca gcttacagcc ccaaagtggg cctgcaccat atccagccgg
     6301 taaccccggt tggctgtttc ttcaaggagg gcgctgatcc tggccgcttc cttatcctcc
     6361 agggcatgag ccctggcgac aggaaccatt tctaccatct ccatcaccct tgcgctggtt
     6421 tcctccattc cctgacggaa ggagcggttt tccctgtgga tcttggctct gaatgccact
     6481 accgc
//
LOCUS       DBMDUH010000151         6449 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_4949_length_6449_cov_7.998267, whole genome shotgun sequence.
ACCESSION   DBMDUH010000151 DBMDUH010000000
VERSION     DBMDUH010000151.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 6449)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6449)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6449
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4949_length_6449_cov_7.998267"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="ACZPCG_11070"
     CDS             <1..702
                     /locus_tag="ACZPCG_11070"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1285632.1"
                     /translation="FRFPDADENTLSHLAFQCEKGTTTAIIGGTGSGKSTIAKLILRF
                     HDVTSGRICLDGTDIRELPQKKLRDAVSYVPQKAWLFSGTIEENLRYGNPNASEAELR
                     TALDTAQAGFVYDLPDGLASHTAQGGTNFSGGQKQRLSIARALAKKAAVYIFDDSFSA
                     LDAKTDAALRHALKDATKDAAVIIIAQRVSTILHADQILVLDDGKIAGLGTHSWLLEH
                     CPVYKDIVRSQLRDA"
     gene            737..2545
                     /locus_tag="ACZPCG_11075"
     CDS             737..2545
                     /locus_tag="ACZPCG_11075"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1285633.1"
                     /translation="MENDQNYFDTEVFEAKHMKKTILRFIRSMGKQKWRLTVVFICIA
                     FYTYFTVMAPLYSADVVDLIWNQMKEAMQTGQHFQITWEFGGRQLFSLLLMFLACGIF
                     YSLQSFLMANFAEKLNLQLRGEISEKLNRLPLSYFDRQQPGAIMSRATNDLEKMSEAL
                     QTGLLRLFMAVGTILGSLSIMAYFDGFLTAVFLFFTAISLLAAKAASQKTLRYAARRQ
                     DCTGKLNSLIEEAYSGRMLIKAFNREEESLAQIRQAAVELADASEKTDWIINAINPGI
                     RVINRFGQVAVAVLGGVMLLKGHLTPGRFQAFFQYANQAGEPITELSYMINSLQSALA
                     SVERVYELLDEEEILPDPEHPACLPAPKGHVDFSHIRFGYTPERTLMSDISFSVKEGQ
                     KIAIVGATGAGKTTLINLLMRFYEIGGGTILLDGVDTRSLSRAGLRQAFGMVLQDTWL
                     FEGTIAENIAYGKPDASREEIIAAAKAARADFFIRTLPKGYDTVLGSDAETISAGQRQ
                     LLTIARVMLCDPAILILDEATSSVDTRTEMEISLAMNTLMKGRTSFVIAHRLSTIVDA
                     DLILVMQNGTIIEQGNHKRLLESGGAYAELYNSQFA"
     gene            complement(2551..4884)
                     /locus_tag="ACZPCG_11080"
     CDS             complement(2551..4884)
                     /locus_tag="ACZPCG_11080"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR1285634.1"
                     /translation="MAYSQKRPAEAKKLRVVGAPLIKRDAKALVTGKPVYTDDLAMKD
                     CLIVKVLRSPYAHALIREIDCAVASKVQGIECILTWKDVPKKRFTMAGQTYPEPSPYD
                     RLILDQRVRFVGDAVAIVAGEDEACVDRAMRLIKVKYEVLEPVLDMHDAKDGDILVHP
                     EEDWKALCPVGADNMRNLCACGKDEAGDLEAAFAGSSHVIERVYHTKANQQAMMETFR
                     AFTYMDIYGRLVVTASTQVTFHVRRILAHALDVPKSKIRVIKPRIGGGFGAKQTVIAE
                     VYPAIVTMKTGKPAKIVYSRYESQIASSPRHEMEVKVKLGCDDEGILQAIDVYTLSNT
                     GAYGEHGPTTVGLSGHKSIPLYGTPKAFRFAYDVVYTNRMSAGAYRGYGATQGIFAVE
                     SAIDELAAELHMDPVKLREKNMIREGQVMPAYYGETAESCTLDRCVDRVKEMIGWDEK
                     YPRKDMGNGKVRGVGIAMAMQGSSISKVDVASVTIKVNDDGFYSMTIGASDMGTGCDT
                     TLAQVAAECLNCEMDDIVVYGVDTDISPYDSGSYASSTAYLTGNAVVKTCETLKKKII
                     KKAAEYLSCGEDELEFTGKSVKRLTPVPEGSGFENEISLLDIGNRAMCFNNEALQATE
                     SCTSPVSPPPFMAGAAEVEVDMETGQIDLIQFVSAVDCGTPLNENLARVQTEGGLVQG
                     IGMALYEDVTYLKNGHVCENSLMQYKLPTRLDIGRVQVELECSYEPTGPFGAKSIGEI
                     VINTPSPAIANAVYNAVGVRIRELPITAEKIFNGLNQ"
     gene            complement(4884..5351)
                     /locus_tag="ACZPCG_11085"
     CDS             complement(4884..5351)
                     /locus_tag="ACZPCG_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR1285635.1"
                     /translation="MEIRFRLNGKQVQAEVRPDTVLLDLVRELGCYSVKRGCETANCG
                     LCTVWLDGKPVLSCSTLALRADGRHVTTLEGLQKEAEEFGMFLAAEGGEQCGFCSPGF
                     IMNVLAMERELKDPTEDEIKEYLAGNLCRCSGYMSQLRAVKKYLDAKQKGGRD"
     gene            complement(5351..6142)
                     /locus_tag="ACZPCG_11090"
     CDS             complement(5351..6142)
                     /locus_tag="ACZPCG_11090"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR1285636.1"
                     /translation="MFRADDYVKVTSLAEAYELCQKRSSLVVGGMVWLKMTNVTKRMV
                     IDLSGLGLDQIEENEEEFSIGAMCSLRQLEIHEGLNTYFGGIFKECTRHIVGVQMRNC
                     ATVGGSVYARFGFSDILTCLLALDAYVELYHGGMVPIKEFAARPVHRDDRDILVRVVI
                     KKDGRKAAYISQRNSSTDFPLLAVCAANLGNQWFVSIGARPAKAALVEFTEDNFDTIK
                     EMAKQAADAVSYGDNTRGSGQYRHSLAVVYVRRLMERLKGRSVSR"
     gene            complement(6237..>6449)
                     /locus_tag="ACZPCG_11095"
     CDS             complement(6237..>6449)
                     /locus_tag="ACZPCG_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HIR1285637.1"
                     /translation="AVRTEDYTAAVKTGTAEFETGKETHAWFTGFAPAEAPKIAVTVL
                     VEESGSGGKIAGPVARAMFDAYLLKK"
BASE COUNT         1479 a         1778 c         1528 g         1664 t
ORIGIN      
        1 ttccgcttcc cggatgcgga tgagaatacc ttaagtcatc tggcctttca atgtgagaaa
       61 ggcactacaa ctgccattat cggcggtacg ggaagcggaa aatccaccat tgcaaagttg
      121 attctgcgct ttcacgatgt tacctccgga cggatttgtc tggatggaac cgatatccgg
      181 gaacttcccc agaaaaagct tcgtgacgca gtatcttatg taccccagaa agcctggctc
      241 ttttccggca ccattgaaga aaacctgcgg tatggaaatc ccaacgcctc ggaggcggag
      301 ctgcggacag ctctggatac agctcaggcg ggctttgtat atgacctgcc ggatggcctg
      361 gcttcccata cagctcaggg gggcacgaat ttttcaggcg gacagaagca gcgcctttcc
      421 atcgccaggg cactggcaaa aaaagccgct gtttatatct ttgacgacag tttttccgct
      481 ctggacgcaa agacagacgc agctctccgc catgctttaa aggatgccac gaaagatgcg
      541 gctgtcatca tcatcgccca gcgtgtaagc accattcttc acgcggacca gatcctggtg
      601 ctggatgacg ggaaaatcgc aggactgggc actcattcct ggcttttgga gcactgtccg
      661 gtatataagg atattgtgcg ttcccagctt cgtgacgcct gattttgtca tattttaagg
      721 gaggttcatt gattttatgg aaaatgacca gaattatttt gatacagaag tatttgaagc
      781 aaagcatatg aaaaagacca ttctgcgctt tatccgctct atggggaagc agaaatggag
      841 actgaccgtt gtatttatct gtattgcctt ttatacctat tttacggtca tggcgccctt
      901 atacagtgca gatgtggtag acctgatctg gaaccagatg aaggaggcaa tgcagaccgg
      961 acagcatttt cagatcacct gggagtttgg cggaaggcag cttttctccc ttcttctcat
     1021 gtttttagcc tgcggtatct tctattccct ccaaagcttt cttatggcaa actttgcaga
     1081 aaaattgaat ctgcagctgc ggggggagat cagcgagaag ttaaaccgtc ttcccttgtc
     1141 ctactttgac cgccagcagc cgggagccat catgagccgg gccaccaatg acctcgaaaa
     1201 aatgtccgag gctcttcaaa cgggacttct gcgcctgttt atggctgttg gaaccatcct
     1261 gggttctctt tctatcatgg cttactttga cggattcctc actgcggtct ttttgttctt
     1321 caccgcaatt tccctgctgg cagccaaagc cgcttcacaa aagaccctgc gctatgcggc
     1381 cagacgccag gactgcacgg gaaaactcaa cagccttatc gaagaagcat acagcggccg
     1441 tatgctgatc aaagccttta accgggaaga ggaaagtctg gcccagatcc gccaggccgc
     1501 tgtggagctg gcagacgctt ccgaaaaaac agattggatc atcaatgcca ttaaccccgg
     1561 aatccgcgtt atcaaccggt tcggacaggt tgctgtggct gttttgggag gtgttatgct
     1621 cttaaagggc cacctgaccc cgggacggtt ccaggcgttc ttccagtatg ccaatcaagc
     1681 cggagaaccc attaccgagc tgtcctatat gatcaattcc ctccagtctg cccttgcatc
     1741 ggtggaacgg gtctatgagc tgctggacga agaagaaatc ctaccggatc ctgagcatcc
     1801 ggcctgtctt cccgccccaa agggtcatgt ggacttctct catatccgtt tcggctacac
     1861 tccggagcgc accctcatgt ctgatatcag cttttccgta aaagaagggc agaaaatcgc
     1921 tattgtaggc gctacgggtg cgggaaaaac cacgctgatc aatctgctga tgcgtttcta
     1981 tgaaatcggc ggcggaacca ttttactgga cggcgtagac acccgctcct taagccgggc
     2041 cggccttcgc caggccttcg gcatggtact gcaggacacc tggctttttg aggggaccat
     2101 tgcggaaaat attgcctatg ggaagccgga tgcttccaga gaagagatta ttgctgctgc
     2161 aaaggcagcc agagccgact tttttatccg tacgctgcct aagggatacg atacggttct
     2221 tggaagcgat gcggaaacta tttccgcagg acagcgccag ctccttacca tcgccagggt
     2281 catgctctgc gatcctgcca tcctgatcct ggatgaggct acctcttctg tggatacccg
     2341 gacggaaatg gagatcagcc ttgccatgaa cacactgatg aagggccgta cttcttttgt
     2401 gatcgcccac cgtctgtcta ccattgtgga tgcggatctg atcctggtaa tgcagaatgg
     2461 tacgattatc gagcagggga atcacaaacg cctcctggaa tcaggaggcg cttatgcgga
     2521 actgtataac agtcagtttg cttaatttaa ttactgattt aaaccattaa atatcttttc
     2581 agccgtaatc ggaagctctc tgatccggac gccgactgca ttgtatactg cattggcaat
     2641 ggccggggac ggagtattga ttacgatctc accaatggac ttggcgccaa aggggcctgt
     2701 cggctcatag ctgcattcca gctctacctg caccctgcca atgtccagac gggtggggag
     2761 cttgtactgc atcagggaat tctcgcagac atggccgttt ttcagatagg ttacatcctc
     2821 gtaaagagcc attccgatgc cctgtaccag tccgccctct gtctgtactc ttgccagatt
     2881 ttcatttaac ggcgtaccgc agtcaacggc tgatacaaac tggatcagat cgatctgtcc
     2941 ggtttccata tctacctcta cttctgccgc acctgccatg aacggaggcg gagatacggg
     3001 agatgtacag gattcggttg cctgaagtgc ctcgttatta aagcacatag ctctgtttcc
     3061 gatatccaga agagaaatct cattttcaaa tcctgagcct tcgggcaccg gagtgagacg
     3121 ctttacggat tttccggtaa attccagttc atcctcgccg cagcttaagt attctgcggc
     3181 cttctttata atctttttct ttaaggtctc gcaggtctta accactgcgt ttccggtcag
     3241 gtaggccgta ctggaagcat aggaaccgga gtcataggga gaaatatccg tatctacgcc
     3301 gtagacaacg atatcatcca tctcacagtt cagacactct gccgctacct gcgccagggt
     3361 ggtatcgcag cccgttccca tatcggatgc accgatggtc atggaataaa aaccgtcatc
     3421 atttaccttg atggttacag aagccacatc tacctttgaa atagatgaac cctgcatagc
     3481 catggcaatt cctacgccgc ggacttttcc atttcccata tcctttctgg gatacttctc
     3541 atcccagccg atcatttcct ttacacgatc cacacagcgg tccagcgtgc agctttctgc
     3601 cgtttctccg taatatgcag gcattacctg tccttcacgg atcatatttt tttcacgcag
     3661 cttaaccgga tccatatgaa gctctgcagc cagctcgtca atggcagact ctacggcaaa
     3721 aataccctgg gtcgcaccat agccgcggta tgcgcctgca gacatacggt tggtgtagac
     3781 tacatcatag gcaaagcgga aggcctttgg agttccgtac agaggaatag atttgtgtcc
     3841 ggaaagtcct acggtcgtcg gcccgtgctc gccgtaagcg cctgtgttgg acagggtgta
     3901 aacgtcgatt gcctgtaaaa tgccttcgtc gtcgcagcct aattttacct ttacttccat
     3961 ctcatggcgg ggagaggaag caatctggga ctcgtagcgg ctgtaaacga tctttgcggg
     4021 ctttcctgtc ttcatggtaa cgatggcagg gtaaacctct gcgattacag tctgcttggc
     4081 tccgaagccg ccgccgatcc tgggcttgat cacacggatc tttgactttg gcacatccag
     4141 cgcatgagcc aggatccggc gcacatggaa ggtaacctgg gtagatgcgg ttacaaccag
     4201 tcttccgtag atatccatat aggtgaaggc ccggaaggtc tccatcatgg cctgctgatt
     4261 tgcctttgta tggtaaacac gttcaattac atggctgctg cctgcaaatg cggcttccag
     4321 gtctccggcc tcatccttac cacaggcaca gagattgcgc atattgtcag cgccaacggg
     4381 acagagggcc ttccagtcct cctcaggatg aactaaaatg tctccgtcct ttgcatcatg
     4441 catatcaaga acaggctcta atacctcata ttttaccttg atcaaccgca tggcccggtc
     4501 tacacaggcc tcgtcctcac ctgctacaat ggctaccgcg tcgcccacga agcgcactct
     4561 ctgatcaagg atcagacggt cataggggct gggctccgga taggtctgtc ctgccatggt
     4621 aaagcgcttc ttcggcacat ccttccaggt aaggatacat tcgattccct gtaccttgga
     4681 ggctacggcg cagtcgatct cacggatcag cgcatgggca taggggctgc gcagaacctt
     4741 tacgatcagg cagtccttca tggcaagatc atccgtatac accggctttc ccgtcacaag
     4801 agctttcgca tctctcttga tcaggggtgc gcctactaca cgcagctttt tggcctcagc
     4861 gggccgcttc tgtgaatatg ccatcagtcc ctgcctcctt tctgctttgc atccagatac
     4921 tttttaactg cacgaagctg ggacatatat ccgctgcagc ggcagagatt tccggctaaa
     4981 tactccttga tctcgtcctc tgtaggatcc tttaactccc gctccatggc cagtacgttc
     5041 ataataaagc ccgggctgca gaagccgcac tgctctccgc cctcagcggc taaaaacatg
     5101 ccaaattcct ctgcctcctt ctgaagtccc tccagagtag ttacatgacg tccatccgct
     5161 ctgagagcca gagtggaaca tgacaatacg ggctttccgt ccagccagac agtacacagg
     5221 ccacagtttg ctgtttcaca gcctcttttt acgctgtagc agcccagttc acgcaccaga
     5281 tcaagaagca ccgtatccgg acgcacctct gcctgaacct gttttccatt taaacgaaag
     5341 cgaatctcca ttaccggctc acactccttc cctttaagcg ctccatgagg cgtcttacat
     5401 acacaactgc caggctatgg cggtactgtc cgctgcctct tgtattatct ccataggaaa
     5461 ctgcgtctgc tgcctgcttt gccatttcct ttatggtgtc aaaattatct tctgtaaatt
     5521 ctactaacgc tgcctttgcc ggtctggctc cgatggatac gaaccactgg ttgcccagat
     5581 ttgcagcgca gactgcaagc agcgggaaat ccgtactgga atttctctgg ctgatatagg
     5641 cagcctttct tccgtccttt ttgatcacca cacggacaag gatatctctg tcatccctgt
     5701 gaaccggacg ggctgcgaac tctttgatcg gcaccatgcc gccgtggtac agctctacat
     5761 aagcgtcaag agccagcagg caggtaagga tatcggaaaa tccgaaacgg gcatatacgc
     5821 ttccgcctac agttgcgcag ttgcgcatct gaactcctac gatatgtctg gtgcactcct
     5881 taaaaatgcc tccgaaatat gtattcagtc cttcatgaat ttccaactgt ctcaggctgc
     5941 acatagctcc tatggaaaat tcctcttcat tttcttcaat ctgatccagc ccaagaccgg
     6001 ataagtcgat gaccatacgc tttgtcacgt ttgtcatctt cagccacacc atgcctccta
     6061 ctaccaggct gctgcgtttc tggcacagct cgtatgcctc agccaggctg gttaccttaa
     6121 cataatcatc tgctcgaaac acctgatcgc tcctttcgtc atttgcaacg tttccttttg
     6181 tatcagacaa acagaggcat tatagcacga aataccgctg ttttctacat agttcgctat
     6241 tttttcaaaa gatatgcgtc aaacatggcc ctggcaacag gccctgctat ctttcctccg
     6301 cttcccgact cctccaccag cactgtaact gcaatctttg gagcttctgc cggggcaaag
     6361 cccgtaaacc aggcatgcgt ttcctttccg gtttcaaatt ccgcagttcc tgttttaact
     6421 gcagcggtgt aatcctctgt acggaccgc
//
LOCUS       DBMDUH010000152         6395 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_4991_length_6395_cov_4.620114, whole genome shotgun sequence.
ACCESSION   DBMDUH010000152 DBMDUH010000000
VERSION     DBMDUH010000152.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 6395)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6395)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6395
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4991_length_6395_cov_4.620114"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(124..315)
                     /locus_tag="ACZPCG_11100"
     CDS             complement(124..315)
                     /locus_tag="ACZPCG_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285638.1"
                     /translation="MVDIYCNGGAFLGVDMDYAILAEVMRDNIIKPNDFIPIQFSDGS
                     RGAVRKKSINGFCESQEEL"
     gene            complement(361..870)
                     /locus_tag="ACZPCG_11105"
     CDS             complement(361..870)
                     /locus_tag="ACZPCG_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HIR1285639.1"
                     /translation="MNFKEAFAKMKQGNKVKLPSWGGYWYWDSEKETIMIQCRSEDSD
                     QGALLDIRDTQRVEYTMMNMQSDDWMIADSKNCPVLGGEATFSFGDAIKYMKRGFRVK
                     RQGWNGKNQYIQLAAGISYKSPEGDIVNCEHEAIGNKAIAFVGTSGVQMGWLASQADM
                     LADDWVFAD"
     gene            complement(882..1178)
                     /locus_tag="ACZPCG_11110"
     CDS             complement(882..1178)
                     /locus_tag="ACZPCG_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HIR1285640.1"
                     /translation="MKKLFISQPMNGRTDEEILKERKEVIKKAESMIGEPVEVIDSFF
                     ETVPENVTPLWYLAKSLELLSTADVAYFAEGWQEARGCKIEHECAVQYGIDRIE"
     gene            complement(1364..1630)
                     /locus_tag="ACZPCG_11115"
     CDS             complement(1364..1630)
                     /locus_tag="ACZPCG_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009245469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6275 family protein"
                     /protein_id="HIR1285641.1"
                     /translation="MGSQEFLNICKAKVAEYYNQKKDKTDTAPVMTVNDVFVVWYSKS
                     LQNHKALLSTPVSDGMYYELTYNGNKKELYFDAYKKWENICYPM"
     gene            complement(1691..1849)
                     /locus_tag="ACZPCG_11120"
     CDS             complement(1691..1849)
                     /locus_tag="ACZPCG_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285642.1"
                     /translation="MAFRRYPEEIEKLFKICEPYEDRVENGELKDAPPEVIEAFEKTK
                     KWAWEQEQ"
     gene            complement(1853..3469)
                     /locus_tag="ACZPCG_11125"
     CDS             complement(1853..3469)
                     /locus_tag="ACZPCG_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002603319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HIR1285643.1"
                     /translation="MEYWQERQEDMYKAGEMQVNKYFTRLEKAFNQTRRELQKTIDAF
                     YFRYAEENGLSFAAAQKKLDAEELGELRDFIDLAMQNIGRYNQQVNNMSIKARITRYQ
                     ALEAQVDAILRQLYAIDYGAEAEKTMQEVYGDTYYRTWYNADQYHGFHAEFAQVSPTV
                     VEKLLEYPFNGAAFSERLWKQKDHLQAQLMEAVTTMLIQGRHPSTLTKDFAKKMQSKK
                     FDAYRLLHTESSFLMSEATHAGYKEDGVEKYEILATLDSKTCNVCGELDNKVYEVGKE
                     VTGVNMPPFHPLCRCTTVPHYDDTPTEGLTRVARDSETGKNYEVPADMSWKEWKKEYV
                     DKQEENQKRKQQAYRPVSRGESTFVEIKPNQKISIQKVDGYSDKVYISDNASIKPRAL
                     HTINLHTQRALEQWGIPLERKPKIVIVSPDELPTAYGKYDAITNTVYYIPQIVDKKVV
                     EKLGDVEYHEMWHMRQAENFRKKHGEIIRENYGKYIEYSCQKAKNVIDQAGIDRYNVG
                     EISDYAKQMYNRQRYDEVEAEYIVANRKKV"
     gene            complement(3469..4950)
                     /locus_tag="ACZPCG_11130"
     CDS             complement(3469..4950)
                     /locus_tag="ACZPCG_11130"
                     /inference="COORDINATES: protein motif:HMM:NF016988.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046798 - viral portal complex [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0051027 - DNA transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HIR1285644.1"
                     /translation="MDILFDPNKNRMTNMQLCRLYMDEFVRSSERKWMLDGEAYYRVD
                     NPEIMNRKMYRYREEKTTGQIEKVLDRSKPNNKRAHGFMHLLIEDKTNYLLSKPYTLT
                     CEESEEYLSMVQDTLGKNFQNRRLMRLGVSASNGGIAWLHPYIDEQGKFQTMIIPPEQ
                     GIPLWRDNDHEELNGFIWFYNVQVIEGQEQKTVTKVEYWLSEAVAYYISDSEGEGWDL
                     RLDSERYLDAVADENREFMEHFHIGNEAGNWGKVPFVPFKNNDYELPDLKFVKSLIDG
                     YDKARSDVANFLDEVRSIVYALKGYGGHDLGEFMRDLNYFRAISLDEDGGAEAITAPV
                     DIAAAKDDFDTLRKDIYDFGQGVDKNSDKFGNNPSGIALKFVYSGLDLKCNRMENAFK
                     AGMERLLCFVDKYLELIGAGSHPGCEIDIVFNRDIAINESQAITDCAASKGIISDETI
                     IKNHPWVEDATEELERLNAQREAEKAELSDMFPPNPDGEDGEA"
     gene            complement(4961..6394)
                     /locus_tag="ACZPCG_11135"
     CDS             complement(4961..6394)
                     /locus_tag="ACZPCG_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HIR1285645.1"
                     /translation="MKERISQTKQKIEKLKGQRNILTKVQIFKFQPFSRKQKQILTWW
                     VPDSPVKDYDGIIADGAIRSGKTVCMSLSFVMWAMSTFSGQNFAMCGKTIGSFRRNVL
                     FWLKLMLKSRGYRVVDHRADNMVEISRGQVTNYFYIFGGKDERSQDLIQGITLAGLFC
                     DEVALMPESFVNQATGRCSVTGSKYWFNCNPDGPYHWFKVNWIDKAIGYLGKKKVAKI
                     REEAKATGKEPDLKKLLYVHFTMDDNLSLSEEIKARYLSVYTGVFFKRYIMGLWAMAE
                     GVIYDMFDPDKHTVDTEALAAAYKAQTGHDFWTGDKYVSCDYGTQNPTAFLFWQQGAD
                     KKWYCRREYYYSGRDKGRQKTDAEFSADLKKWLGNIEIRAVILDPAAASFKAQLEKDG
                     FKVKKAKNDVLDGIRLVATLLLTGSIFIDKSCENLIKEFASYIWDAKAADKGEDKPVK
                     EHDHALDALRYFCMTIIKMRPGIRILK"
BASE COUNT         1518 a         1780 c         1192 g         1905 t
ORIGIN      
        1 ttcggttgct cctttcgatt catcttcgct tgttatcccg gtcgcgtccg gtgatgtccc
       61 gttctttttc gcctgcggta ccggaaaggc gaaaaaataa cgctcaggtc tatacctgaa
      121 cgcttataac tcttcttggc tttcgcaaaa gccatttatg cttttctttc taacagctcc
      181 tctgcttcca tcagaaaatt ggataggaat aaaatcattc ggctttataa tgttatcccg
      241 catgacctct gccaaaatgg catagtccat atcaacccca agaaatgctc cgccattaca
      301 ataaatatca accatagggt tcacctgcgt gtctatgact aattttataa cttgctatga
      361 ctaatcagca aatacccagt catccgccaa catatccgcc tgtgacgcaa gccagcccat
      421 ctgaactcca cttgtaccta caaaagcgat agccttattt ccaattgctt catgctcaca
      481 attcacaata tctccttcgg gagacttata ggaaatccca gcagcaagct ggatatactg
      541 attttttccg ttccatccct gtcttttaac cctaaatcct cgcttcatgt acttaatagc
      601 atcaccaaaa gagaatgttg cttctccacc aagcactgga caattcttac tatcagcaat
      661 catccagtca tcggactgca tattcatcat agtgtactcc actctctggg tatcacggat
      721 atcaagaagc gctccctgat cgctatcctc tgatcggcac tgtatcataa tggtttcttt
      781 ttctgaatcc caataccaat atcctcccca tgatggcaat tttactttgt tcccctgctt
      841 catctttgca aatgcttctt taaaattcat tttccaatct cctattcaat tctgtcaatt
      901 ccatattgta ccgcacattc atgctctatc ttgcaccctc tggcctcctg ccaaccctct
      961 gcaaaatatg ccacatctgc cgtggacagc aattccagtg attttgccag ataccacaga
     1021 ggcgtaacat tctctggaac agtctcgaaa aaagaatcaa tcacttctac tggttccccg
     1081 atcatgcttt cagctttttt gataacctct tttcgttctt ttaaaatctc ttcatctgtc
     1141 ctgccattca taggctgtga tataaataac tttttcatct cctgatctcc ttcccacatt
     1201 tcacacaccg cctcacatac ccgccataag ggccatggcg gcggcaccag tgctttctat
     1261 actgatgttc acatccaggc tttgcccgac agaagaaccg tttcagccat cttaaaaatc
     1321 tacccataat tctctccttc ctgttgcgac gtcgcaacgc ccattacatg gggtaacaga
     1381 tattttccca cttcttataa gcatcaaagt acagctcttt tttgttgccg ttgtacgtca
     1441 actcataata cataccatca gatacaggcg tactgagcag ggccttatga ttctggaggc
     1501 tcttgctgta ccagactaca aacacatcat tcaccgtcat aaccggagct gtgtctgtct
     1561 tatccttctt ctggttgtaa tactctgcta ccttggcttt gcagatgttt aaaaactcct
     1621 gacttcccat cgtaattcct ccttaatttt gcgtacaaaa ataccaccgg ccattactga
     1681 ctggtggtat ctactgttcc tgttcccaag cccatttttt tgttttctca aatgcctcta
     1741 ttacttctgg gggtgcatct ttaagctctc cattttcaac cctgtcttca tagggttcac
     1801 atatcttaaa taacttttca atctcttcag gataccgacg aaacgccact gatcacacct
     1861 tctttctgtt tgctacgata tactcagcct caacttcatc atatcgctgt cggttataca
     1921 tttgtttagc ataatcacta atctcaccaa cattataccg atcaattcca gcttggtcaa
     1981 ttacattctt cgccttttgg caagaatatt ctatgtattt cccataattt tctcgaatga
     2041 tctcgccatg tttctttcgg aagttttctg cctgcctcat gtgccacatt tcgtgatact
     2101 ctacatctcc caatttttcc acaacttttt tatcgacaat ctgtggaata taatacacag
     2161 tgttagttat tgcatcatat tttccataag ctgttggcag ctcatccgga gaaacgatga
     2221 ctattttcgg ttttcgctct aaaggaattc cccactgttc taaggccctt tgcgtatgca
     2281 gatttatcgt gtgcagtgca cgtggtttga tgcttgcatt gtccgatata taaactttat
     2341 ccgaataacc atcaaccttt tgaatgctga ttttctggtt tggttttatc tccacaaatg
     2401 tgctttcacc tcttgaaact ggtctgtatg cctgctgttt ccgcttctga ttctcttcct
     2461 gcttatcaac atactccttt ttccactcct tccagctcat atctgctgga acctcatagt
     2521 tctttcctgt ctcagaatcc ctggccactc tggtcaatcc ttcggtcgga gtatcatcat
     2581 agtgcgggac agtcgtacag cggcacaatg ggtggaaggg aggcatatta acgccagtga
     2641 cctcttttcc aacctcatac accttattgt ccagttctcc gcaaacattg caagtcttac
     2701 tatccagtgt ggcaaggatc tcatatttct ccacaccatc ctctttgtat ccggcgtggg
     2761 tggcctcact catcaggaag gaactctctg tatgcaggag ccggtaagca tcgaactttt
     2821 ttgattgcat cttcttggca aaatccttag tcagcgtgga tgggtgtctg ccctggatca
     2881 gcatcgtggt cactgcttcc atcagctggg cctgaagatg gtccttctgt ttccagagcc
     2941 gttcggaaaa tgctgcacca ttgaacggat attccagaag cttttccaca acagttgggc
     3001 ttacctgtgc aaactccgcg tgaaaaccat gatactgatc tgcattgtac caggtacggt
     3061 agtaagtatc cccatagact tcctgcatgg tcttttccgc ctctgcccca tagtcgatgg
     3121 catacagctg ccggagaatc gcgtctacct gtgcttccag agcctgatag cgggtgatcc
     3181 gggccttgat agacatatta ttgacctgct gattgtaacg cccaatattc tgcatggcca
     3241 gatcgataaa gtcccgcagc tctcccagtt cctctgcatc cagcttcttc tgggcggctg
     3301 caaaggatag cccgttctcc tctgcatacc ggaagtaaaa cgcatctatg gtcttctgga
     3361 gttctctacg cgtctgatta aaggcttttt ccagacgtgt gaagtatttg ttgacctgca
     3421 tctccccggc cttgtacatg tcctcttggc gctcctgcca gtattccatt aagcctcacc
     3481 gtcctcgcca tctgggttcg gcgggaacat atcggataat tccgccttct cggcttcccg
     3541 ctgggcgttt aaacgctcaa gctcttctgt agcatcctca acccacggat ggtttttaat
     3601 gatagtctcg tctgaaatga tacccttgga tgcggcgcag tcagtaatag cctggctctc
     3661 gttaattgcg atatcccggt taaagacaat gtcgatctca catcctgggt gactgccagc
     3721 cccgatcaat tccagatact tatccacgaa acaaagcaac cgttccatgc ccgccttaaa
     3781 cgcattctcc atccggttgc atttcaaatc caaaccagag tacacaaact tcaaggcaat
     3841 accagaaggg ttattaccaa atttatcact gttcttgtct acgccctgtc caaaatcata
     3901 gatatctttc cgcagcgtgt caaagtcgtc cttggccgct gcgatatcca caggtgctgt
     3961 gatcgcctct gccccgccat cctcatccag agagatcgcc cggaaataat tcagatcccg
     4021 cataaactcg cccagatcat ggccgccgta tcctttcaag gcatacacaa tagaacgtac
     4081 ctcatcaagg aaattggcta catcagaccg ggccttgtca tatccatcaa tcaacgactt
     4141 tacaaattta agatccggca gctcgtagtc attgttttta aatgggacaa atggaacctt
     4201 gccccaattt ccggcctcgt tgccgatatg aaaatgctcc ataaactcac ggttctcatc
     4261 tgccaccgca tccagatagc gctcagaatc caaccgtaag tcccatccct cgccttctga
     4321 atcagagata tagtaagcga cggcctccga aagccagtat tccacttttg tgaccgtctt
     4381 ctgctcctgt ccctcgatga cctgaacatt ataaaaccag ataaagccat tcagctcttc
     4441 gtgatcgtta tccctccaaa gtgggatccc ttgttcaggc ggaatgatca tagtctggaa
     4501 ttttccctgc tcatcgatat acggatgcag ccatgcaatc ccgccattgc tggcagatac
     4561 acccagacgc atcaggcgcc tgttctgaaa gtttttccct aaggtatcct gtaccataga
     4621 cagatactct tctgattcct cgcaggtcag tgtataaggc tttgaaagca gataattggt
     4681 cttatcctca atcagcaagt gcataaaacc atgcgcacgc ttattattag gcttggatct
     4741 atccagtacc ttttctatct gcccggtagt cttctcttcc cggtagcggt acatcttccg
     4801 gttcatgatc tccggattgt ctacacggta atacgcctcc ccgtccagca tccacttccg
     4861 ctctgaagat ctcacaaact cgtccatgta cagtcggcat agctgcatat tagtcatccg
     4921 gtttttattt gggtcaaata atatatccat ccaatccacc tcacttcaaa atccggattc
     4981 ccggacgcat cttaattatt gtcatgcaga aatacctgag ggcgtcaagt gcgtgatcgt
     5041 gctctttcac cggtttgtcc tctcccttgt ctgctgcttt tgcgtcccag atataggatg
     5101 caaactcttt gatcagattt tcgcaagact tatctataaa aatagaaccc gtaagcagca
     5161 gcgtagccac caaccggatc ccatctaaaa catcattttt cgctttttta actttaaagc
     5221 cgtccttttc caactgggcc ttgaagctgg cggctgccgg atccagaatt acagctctga
     5281 tttctatatt ccccagccat ttttttaagt ctgctgaaaa ctctgcatct gttttttgcc
     5341 tccctttatc tcttccagaa taatagtatt cacggcggca gtaccatttc ttatcagctc
     5401 cctgctgcca gaataggaag gccgtcggat tctgggtacc atagtcacag ctgacatact
     5461 tatcgccggt ccagaagtca tgccccgtct gtgccttata tgcggcagcc aaagcctctg
     5521 tatcaacggt gtgcttatcc ggatcaaaca tatcgtagat gacgccctca gccatcgccc
     5581 agagtcccat gatatagcgc ttgaagaata cgccggtata tacgctcagg tacctggcct
     5641 tgatctcttc cgacaggctc aggttgtcgt ccatagtgaa gtggacatac agcagcttct
     5701 taagatccgg ttccttccct gtggcctttg cttcctcacg gatcttggcg acctttttct
     5761 tccccaggta gccgatggcc ttatcaatcc agtttacctt aaaccagtga taggggccgt
     5821 ccgggttgca gttgaaccag tactttgatc ccgtcacgga gcagcgacca gttgcctggt
     5881 tgacgaagct ctcaggcatc agcgccacct catcacagaa caggcccgcc agggtaatgc
     5941 cctgaatcag atcctgactg cgctcatcct taccgccgaa gatgtagaag taattcgtga
     6001 cctgtcccct gctgatctcc accatgttgt cggctcgatg atccaccaca cgataaccgc
     6061 ggcttttaag catcagcttc agccaaaata aaacgttacg ccgaaaggag ccgatggtct
     6121 ttccgcacat ggcaaagttc tgacctgaga aagtactcat ggcccacatc acaaaagaca
     6181 gggacataca gacggtctta ccggatcgga tggccccatc tgctataatg ccgtcataat
     6241 cctttacagg gctgtcaggc acccaccatg tcaggatctg cttctgcttg cgggaaaatg
     6301 gctgaaactt gaagatctga acctttgtca ggatattgcg ctggcctttc agcttttcaa
     6361 tcttctgttt tgtctgactg atccgctcct taatc
//
LOCUS       DBMDUH010000153         6363 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_5018_length_6363_cov_5.751677, whole genome shotgun sequence.
ACCESSION   DBMDUH010000153 DBMDUH010000000
VERSION     DBMDUH010000153.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 6363)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6363)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6363
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5018_length_6363_cov_5.751677"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            271..1074
                     /locus_tag="ACZPCG_11140"
     CDS             271..1074
                     /locus_tag="ACZPCG_11140"
                     /inference="COORDINATES: protein motif:HMM:NF040203.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285646.1"
                     /translation="MKKWKSIRLMCLIGAVLLQMENMDALAAEPGGSDPGWICLDGNW
                     YWQKADMTAATGWLSAGGRTYYLREDGVMATGFQKVGNEWYYFHEDGGMNLGDLELDN
                     AVYTFSAEGALTGASWKENTGGGAYFAGCYDTQEQILFEYLCDEKRDQYFDARPDREH
                     EYDGDMHTSYDRYAGLKMDVKLNRIAAARLKEAMENGYADGKNSTYKELYIRNCEDGE
                     AAFDKIMDVLEKQYSRGEAYQDTLDYYRYLGMAHYEADGRHWFMVVLKR"
     gene            1111..3318
                     /locus_tag="ACZPCG_11145"
     CDS             1111..3318
                     /locus_tag="ACZPCG_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003510418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR1285647.1"
                     /translation="MKEKLKFVHKKKEKQTENGLTERLSVRLARFILRRQKWIESIFV
                     AGCVFSFIAMLFVNVNYDLTEYLPREAASRIGLDRMEETFGYPGTARVMVKDVSLYEA
                     KQYKDQIEAVDGVDQVLWCDAAVNIYAGEDFISMDDIEDYYKDRCAVMDITFDEGDTA
                     KRTSRAVDEIRNLLGEKGCYTGMAVQNKSLKENIESEMQLIMLVAVIMIFTILCITTA
                     AWSEPFLFLIVMGVAILLNQGTNIFLGTVSFLTNNVAMVLQLATSMDYSIFLLDAFER
                     ERKKGLGNKEAMTNAIDAAINSIFASSLTTVVGFLALVSMKFSIGFDMGLVLAKGIVF
                     SLLTVVFFMPAMILKFADWNEKTAHRPFLPAFNRLSRGIYRFRYAALIIMALLAPPAY
                     VAQGMNDYLYGNSAVGASEGTRVYEDEKEIAAIFGRSNMLLVMYPNTSMVTEKALSGE
                     LEDLPYVKSVTSMANTLPEGIPEEFLPYSLTNELHRGDKGRMLVYIRTKTESREAFQD
                     TGKIEEIVKKYYPEDSFVLGEAPSTMDIKTFITADNTRVNILSLLGVFLVVMFSFRSL
                     LVPVIVMIPIEAAIFLNMAMPYLAGDTMVYMGYIIVSSIQLGATVDYSILLTNSYMAC
                     RKEMEKKKACIQAVKMSCTSIFTSGTIMILAGYIIHFISNTAAIGDLGHLIGRGALFS
                     VILVLTVLPSLLVLFDSIITRKEWERIRKKFMGKRRSLTDSKMEGMEAKSNEN"
     gene            3308..5533
                     /locus_tag="ACZPCG_11150"
     CDS             3308..5533
                     /locus_tag="ACZPCG_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285648.1"
                     /translation="MKIRKRQMMAAVLAAVVTAGNAPLSYGAPAAVETDETMYVNLDV
                     YGKAQKVNVVKSCSLNGVTDFTDYGNYLAVENMSTQDEPQLGEGEVVWKLPPDRRERF
                     YYKCTLDQEQTALPWNFDVSYKLNGVPTDGEKLAGASGLVEIHIKAEPNEQADLYYRN
                     NMLLLVAVPVDMSKCFSVEAEGSQTQNMGELTAVVFTALPGEEGDYTVRIGSDSFETT
                     GVIMAMTPGTVEDLEHIKDLKEAKDTWQDAGDELYDSLEQMAQSIEDMRGGVDQMQSG
                     LNAAENARQKWSSSKDAILAGNDRSLESLRAMSGQLETLVPHLQTAKEEAEIVHKSMG
                     DIVGTLGEMQEPLGKLYTRLRNIKSSTASMGEQLPDLRDDLGYLIETNASFQVQTTEA
                     LEALGQLIGELEEYDDEALEIEVPEIEIPEDQGEEELEEPEASEPEGSEKPEESEEQE
                     KPEGSEEPEAPDASEPEKDENLEINEPENENTGSQTISRAVVPKAQIEKREVPFVASS
                     GSQLQQLMQMLRKIDKDSRKFTQTAGNLMEDISDSAQYSADLVDNMDFLIEDLTALYD
                     SLDTYYPDLQSALDDLSALTERTTEALNSTTDTLTLVQNTLKASSDEMDVAAKDSLMA
                     SMELLNKSLNVLDSTAGIRRAGRTMKDVIDSQLDKFDTENRFLFMDPDAEKVSFTSSK
                     NPEPRTLQVVLRTDEISLENEEHKTLDAETEEPEVSPLKRMWNVLVKMWRSLVDIFKN
                     R"
     gene            5616..6179
                     /locus_tag="ACZPCG_11155"
     CDS             5616..6179
                     /locus_tag="ACZPCG_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HIR1285649.1"
                     /translation="MELTKQEKTKYRLAASVKECMKTAPVDKITVKNIVEGCGLTRQT
                     FYRNFLDKYDLINWYFDKLVLQSFEQIGMGHTVGESLTQKFAFIRAEKVFFTEAFRSD
                     DRNSVKEHDFELILQFYRDLINRRTSRPLSEEIQFLLEMYCRGSVYMTVKWVLGGMKD
                     SPEEMAKKLVEALPPKLARVFDELQLL"
BASE COUNT         1807 a         1189 c         1770 g         1597 t
ORIGIN      
        1 agtcttttaa ttgtttccgc gttttgcgga ttcttcggtc gatcagttct gttcccattt
       61 tcataacctc cttatgtatg ggattttgta taataatgaa cataaaaatc gaatatgttg
      121 attaacgtac attgaatgtg aaatcaatca ttgttatttt aaacattcat tagtataatc
      181 tattatagtt aagtaatgaa catatgtcaa ttatcgttca cagagttgag aaaataaaca
      241 tgaccagcaa agaatcagga gatcggttgt atgaaaaaat ggaagagtat caggttgatg
      301 tgcctgatcg gggcggttct gcttcaaatg gaaaatatgg atgcgctggc ggcagagccg
      361 ggaggttctg atccgggatg gatctgtctg gatggaaatt ggtattggca gaaagcggat
      421 atgacagcag ctacaggctg gctttcagct ggaggaagaa cttactatct gagggaggac
      481 ggggtcatgg cgaccggatt tcaaaaggta ggaaatgagt ggtattattt ccatgaggac
      541 ggtggaatga atctgggaga tctggagctt gataatgcgg tatatacctt ttcggcagaa
      601 ggtgctctta ccggagcctc atggaaggaa aatacgggag gaggagccta ttttgcaggc
      661 tgctatgata cgcaggagca gattttgttt gagtatttat gcgatgaaaa gcgggatcag
      721 tattttgacg ccagaccgga ccgggaacat gagtatgacg gggatatgca tacgtcctat
      781 gaccggtatg cgggcttaaa aatggatgta aagctgaacc ggattgcggc tgcccgcctt
      841 aaggaggcca tggaaaatgg gtatgcagat gggaaaaaca gcacttataa ggagctttat
      901 atacgaaatt gtgaggatgg ggaagctgct tttgataaaa tcatggatgt gctggaaaaa
      961 cagtacagca gaggagaagc ttatcaggat acgctggatt attatcggta tttgggtatg
     1021 gcccattatg aggcggacgg acgtcactgg tttatggtgg tactgaagcg ctgaatgaaa
     1081 tgaaccccgg gatgttagga gcaggatcaa atgaaggaga aattgaaatt cgtacataaa
     1141 aagaaagaga agcagacaga gaatggcttg acggaacggt tgtctgtccg ccttgcccgc
     1201 tttattttaa gaaggcagaa atggattgaa agcatatttg tggcaggatg tgttttttcc
     1261 tttattgcaa tgctgtttgt aaatgtgaat tacgatctga ctgaatatct gccaagggag
     1321 gcggcatccc gtattggatt ggaccgtatg gaggagacgt tcggatatcc gggaaccgca
     1381 cgggtgatgg taaaggatgt aagtctttat gaggcgaagc agtataagga tcagatagag
     1441 gctgtggacg gagtggatca ggtactctgg tgtgatgctg ccgttaatat ttatgcagga
     1501 gaggacttta tctccatgga tgacatagag gattactata aggatcgctg cgcagtaatg
     1561 gatattactt ttgatgaagg agatacggcc aagagaactt cccgtgctgt ggatgagatc
     1621 cggaatcttt taggggaaaa gggctgctat accgggatgg ccgtgcagaa caaatcctta
     1681 aaggaaaata tagaaagcga aatgcagctg atcatgctgg tagcggtgat catgattttt
     1741 acgatcctat gtattaccac ggccgcctgg tccgaaccgt tcctgttttt gatcgttatg
     1801 ggcgttgcca ttcttttaaa tcagggaacc aatatttttc taggaactgt atcctttctt
     1861 accaacaatg tggctatggt gctgcagctt gcaacgtcca tggattattc cattttcctg
     1921 ttggatgcat ttgaaaggga gaggaaaaag gggctgggaa acaaagaggc catgacaaat
     1981 gccattgacg ctgccatcaa ctctattttt gccagcagcc ttactaccgt agtaggcttt
     2041 ctggctctcg tatccatgaa gttcagcatt ggttttgata tgggactggt gcttgcaaag
     2101 gggattgtat tcagtcttct tactgtggta ttttttatgc cggccatgat cttgaagttt
     2161 gcagactgga atgaaaagac ggctcaccgt ccctttcttc cggcgtttaa ccgtttaagc
     2221 cggggaattt accggtttcg ctatgcggcc ttgatcatta tggctctttt ggcgcctccg
     2281 gcttatgtgg cccaggggat gaatgattat ctttacggaa acagtgcagt gggtgccagt
     2341 gagggaacaa gggtttatga ggatgagaag gagatagcag ccattttcgg gcggagcaat
     2401 atgctcctgg tgatgtatcc aaacacctcc atggtgacgg aaaaagcact gtccggggag
     2461 cttgaggatc ttccctatgt aaaaagcgtc acttccatgg ccaatacgct tccggagggt
     2521 atccctgagg aatttcttcc atattctctt accaatgagc ttcacagggg agataaaggc
     2581 aggatgctgg tgtacatccg tacaaagaca gagagcaggg aagcatttca ggatacaggg
     2641 aagattgaag aaattgtaaa gaaatattat ccggaggatt cttttgtgct gggagaagct
     2701 ccctctacta tggatattaa aacctttatt acagccgata atacacgcgt aaatatcctg
     2761 tccctgctgg gcgtattttt agtcgtcatg ttcagtttcc gttctctttt ggtgccggtg
     2821 atcgttatga tccccattga ggcggctatt ttcctgaata tggcaatgcc gtatctggcc
     2881 ggagatacca tggtatatat gggatatatc attgtgagca gtattcagct gggagccact
     2941 gtggattatt ctatcctgct gacgaacagc tatatggcat gccgaaaaga gatggagaag
     3001 aaaaaagcct gcattcaggc agtgaaaatg tcgtgtactt ccatttttac ctcaggaact
     3061 attatgattc tggccggtta tattattcat tttatatcga atacggctgc catcggagat
     3121 ctggggcatc tgatcggccg tggagctctt ttcagtgtga tcctggtgct gacagtgctt
     3181 ccgtcccttc ttgttctctt tgattcgatc attacaagaa aagaatggga acggatacga
     3241 aagaagttta tgggaaagcg gcgttctttg acagacagca aaatggaagg aatggaggcg
     3301 aagagcaatg aaaattagaa aaagacagat gatggcagct gttcttgcag ctgtggttac
     3361 agccggaaat gcgcccctgt cctatggcgc tccggctgca gtggaaacgg atgagaccat
     3421 gtatgtgaat ctggatgttt acggaaaggc tcaaaaggta aatgtagtaa agagctgcag
     3481 tttaaacggc gtcacagatt ttacagatta tggaaattat ctggcggtgg aaaatatgtc
     3541 aactcaggat gaaccgcagc tgggagaggg ggaagtggtc tggaagcttc cgccggatcg
     3601 cagggaacgc ttttactata aatgtacgct ggatcaggag cagacagcgc ttccgtggaa
     3661 ttttgatgtg tcctataagc tcaacggggt tcccacagac ggggaaaagc tggccggagc
     3721 ttccgggctg gtggaaattc acataaaggc tgagcctaat gaacaggcag atctgtatta
     3781 ccgtaataat atgcttttgc tggtggcagt tcccgtggac atgtccaagt gcttcagcgt
     3841 ggaagcagag ggatctcaga cccagaatat gggggagctt acagcggttg tatttacagc
     3901 tcttccgggg gaagaggggg attatacggt gcgcatcggt tcggattcct ttgagacaac
     3961 gggagtcatc atggcaatga cgccgggaac ggtggaggat ctggagcata ttaaggattt
     4021 aaaagaggca aaggatacct ggcaggatgc gggagatgaa ctgtatgata gcttagagca
     4081 gatggctcag tccatagagg atatgagggg cggagttgat cagatgcagt ccggcctgaa
     4141 tgcagcagag aatgcaaggc agaaatggag cagctcaaag gacgccatac tggcaggaaa
     4201 cgatcggtca ctggaatccc tcagggctat gtccggccag ctggaaaccc tggtgcctca
     4261 tctgcagacg gcaaaggaag aggcagagat cgtacataaa agtatgggcg acattgtggg
     4321 aacactggga gagatgcagg agcctctggg aaagctgtat acaagactga gaaatattaa
     4381 gagcagtacg gcttcgatgg gagaacagct gccggatctg agagacgatc tgggttatct
     4441 gatagaaacg aacgcatctt ttcaggtgca gaccacagag gctttggagg ctctgggaca
     4501 gctgattggg gagctggagg aatatgatga tgaggccttg gaaatagaag tgccagagat
     4561 tgaaattcca gaggatcagg gagaggaaga gttagaggaa ccggaagctt cggaaccgga
     4621 gggctcagag aaaccagagg agtcggagga gcaggagaag ccggagggct cagaagaacc
     4681 ggaggcaccg gacgcttcgg aaccagagaa agacgaaaac ttggaaatca atgagcctga
     4741 gaacgagaat accggcagcc agaccatcag ccgtgcggtt gttccaaagg cacagattga
     4801 aaaacgagag gtgccttttg tggcttcttc aggctcccag ctgcagcagc tgatgcagat
     4861 gctccgaaaa atcgacaaag acagccggaa atttacccag acagccggaa acctgatgga
     4921 ggatatttcc gattctgccc agtacagtgc ggatctggtg gacaatatgg actttcttat
     4981 tgaggatctt acggcgctct atgattcgtt ggatacatac tatccggatc ttcagagcgc
     5041 attggacgat ctgagtgcac ttacggaaag aaccacggag gctttaaaca gcactacaga
     5101 taccctgaca ctggttcaga atacgcttaa agcctccagc gacgagatgg atgtggcagc
     5161 aaaagacagc cttatggcaa gtatggagct tttaaataaa agtctgaatg tgcttgacag
     5221 tacagccggg atccgtcggg ccggccgtac catgaaggat gtgatcgaca gccagcttga
     5281 taaattcgat acggaaaaca gattcctgtt tatggatccg gacgcggaaa aggtatcctt
     5341 tacatcttct aaaaatccgg aacccagaac cctgcaggtt gtgctccgca cagatgagat
     5401 cagtttggag aatgaggaac acaagacgct ggacgcagaa acagaggagc cggaggtaag
     5461 tcctctgaaa cggatgtgga acgtactggt aaaaatgtgg cgttctctgg tggacatctt
     5521 taaaaacaga tagaaaagca gctgattttc tggtatgctt aagaaagagc aggcggtatt
     5581 ctctttgcac agcagtgccg gaaaggatga gcttcatgga acttacaaaa caggaaaaaa
     5641 caaaataccg gctggcagct tcggtgaagg agtgtatgaa gactgctccg gtagataaga
     5701 taaccgtaaa aaatattgtg gaaggctgcg gccttacccg ccagaccttt taccggaatt
     5761 ttctggataa atacgactta attaactggt attttgacaa gctggttctt cagtcctttg
     5821 agcagatcgg catggggcat actgtgggag aaagcctgac acagaagttt gcgtttatcc
     5881 gtgcagaaaa ggtcttcttt acggaagcat ttcgaagcga tgaccgcaat tccgtaaagg
     5941 aacatgattt tgagctgatc cttcagtttt acagggatct gatcaaccgc agaaccagcc
     6001 gtcctttaag tgaagaaata cagttccttc ttgagatgta ctgccgtggt tccgtctata
     6061 tgactgtaaa atgggtttta gggggcatga aggactcgcc tgaggaaatg gctaaaaagc
     6121 tggtagaagc cctgcccccc aagcttgcca gagtatttga cgaattgcag cttttgtaac
     6181 atctgcataa accattttgt gacaattcac aaaaaagaca tagaaagtga cagactaccc
     6241 tgtttgtcac ttttattttt tttaggcgct gttataatat taacaaacag ggaagatcct
     6301 gagaaagaaa aggagaagag gaaccatggc aaacagaatt gtattaaatg aaacttctta
     6361 tca
//
LOCUS       DBMDUH010000154         6240 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_5103_length_6240_cov_5.589673, whole genome shotgun sequence.
ACCESSION   DBMDUH010000154 DBMDUH010000000
VERSION     DBMDUH010000154.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 6240)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6240)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6240
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5103_length_6240_cov_5.589673"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(78..518)
                     /locus_tag="ACZPCG_11160"
     CDS             complement(78..518)
                     /locus_tag="ACZPCG_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285650.1"
                     /translation="MISEKNLIGNVKEKKSNYDLMTAVMICLGKKASTDTNVLKLLNV
                     LLSTETASEDKCQILEEDFKIKMTQTLESEVSLMCNLSKGVEEQGIQKGLQKGLEQGI
                     QAMIATLKKLQIPTETILIETCEKFGLTREDAEKYLKETVSSFV"
     gene            complement(515..889)
                     /locus_tag="ACZPCG_11165"
     CDS             complement(515..889)
                     /locus_tag="ACZPCG_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285651.1"
                     /translation="MNTDTTIAKNIRVTDEKASYDAACKRLLSEKIILAWIMKNCVEE
                     YKNCDVNEIAENYIEGQPQVGETAVAPDETNQTSRIHGTGVEDATLSEGTITYDIRFL
                     AAAPVSGELIRRRTVKTALPVM"
     gene            complement(1028..1318)
                     /locus_tag="ACZPCG_11170"
     CDS             complement(1028..1318)
                     /locus_tag="ACZPCG_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003523167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285652.1"
                     /translation="MNETTSLTCQTQCPTACPCPILLSIASSIAHEEDALACILNAEC
                     AKINRVIAASSDYEALIAIDTSVQATLERVIELEGILKTKLDSILPFLNECI"
     gene            complement(1408..2874)
                     /gene="abc-f"
                     /locus_tag="ACZPCG_11175"
     CDS             complement(1408..2874)
                     /gene="abc-f"
                     /locus_tag="ACZPCG_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HIR1285653.1"
                     /translation="MSMIKAEHLTFSYPSSSDPIFEDVNFQIDTDWKLGFVGRNGRGK
                     TTFLNLLMGKYEYRGTIHSSVSFDYFPYEVSDPSKMTLEIMYEICPDAQDWEFMKELS
                     CLQTDCELLWRPFSTLSKGEQTKALLAALFLNQGHFLLIDEPTNHLDAAARETVAAYL
                     KKKKGFILVSHDRFFLDSCVDHILSLNRADIEVQSGNFSSWMTNFTYRQACELAQNEQ
                     LKKEIGRLKQASARTAGWSDRIEASKIGSGCADRGFVGHKSAKMMQRAKSIQSRQQKA
                     IEEKSGLLKNTETAEALKLLPLRYRSQTLISCCDLSIRYGDRAVCGPFSFTLEQGERL
                     ALNGKNGSGKSSLLKLILGEDIKKTGTISIGSGLIISYVSQDTSHLSGTLSDYASQCR
                     IDESLFKAILRKLDFERIQFTKDMAQFSGGQKKKVLIAKSLCEQAHLYLWDEPLNFID
                     IYSRMQIEQLILDFRPTMIFVEHDRAFRETIATKTLEL"
     gene            complement(3123..3363)
                     /locus_tag="ACZPCG_11180"
                     /pseudo
     CDS             complement(3123..3363)
                     /locus_tag="ACZPCG_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008752575.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
     gene            complement(3356..3625)
                     /locus_tag="ACZPCG_11185"
     CDS             complement(3356..3625)
                     /locus_tag="ACZPCG_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000987 - cis-regulatory region
                     sequence-specific DNA binding [Evidence IEA];
                     GO_function: GO:0015643 - toxic substance binding
                     [Evidence IEA];
                     GO_process: GO:0044010 - single-species biofilm formation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HIR1285654.1"
                     /translation="MAQATLTARVDEKDKINFDAFCSNVGLNTSTAINLFVKAVLREN
                     RIPFEIAQSSDPFFSQSNLEYVKKSIQELRTGKGTAHELIEVDDE"
     gene            3837..4325
                     /locus_tag="ACZPCG_11190"
     CDS             3837..4325
                     /locus_tag="ACZPCG_11190"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR1285655.1"
                     /translation="MERSKLTTLCYMEREGEWLMLHRVSKKNDVNKDKWIGIGGHFEE
                     NESPEECLLREVKEETGLTLTSWRFRGLVTFRSTGGPVEDMEYMCLYTADGFEGEMAD
                     CNEGTLEWVKKEAVCGLNIWEGDKIFFRLLNENAPFFSLKLTYEGDRLIEAVLDGKAL
                     IA"
     gene            complement(4322..5965)
                     /locus_tag="ACZPCG_11195"
     CDS             complement(4322..5965)
                     /locus_tag="ACZPCG_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HIR1285656.1"
                     /translation="MEKIYPMLFTTWKTYSKEQFLKDLIAGIVVAIIALPLSIALALA
                     SGVGPEQGIYTAIVAGFIISFLGGSQVQIAGPTAAFATIVAGIVARNGMDGLAAATIL
                     AGIILIIMGLCRFGGLIKYIPFTITTGFTSGIAVTIVIGQLKDFFGVTYPEGMPTIET
                     MEKLKAFLAGIHTFNTDSLIVGLVCLAILIIAPRFTEKIPGSLLAVIGGILMVQLLPL
                     QVNTIGDLYTISNKLPSFHMPQLSFEMIQTALPDAFTIAILAAIESLLSCVVADGMIN
                     GKHRSNMELAAQGAGNIASALFGGIPATGAIARTAANVKNGGRTPVAGMIHAVVLVLI
                     LIILMPYAAMIPMPAIAAILFMVAYNMCQWRTFTELVKTAPKSDIAVLILTFLLTIVF
                     DLVIAIEIGLLVAVLLFMKRMSDVTEVYSWKYAEDEEEAEREKLRKLSPHINVYEISG
                     PLFFGAADVIGRIAVKDHAKCLILRMRSVPALDSTALNAMTDLYKNCEKRGITLILSH
                     VNQQPMKVMEKAGFAQKVGQENFCENIDRAIKRAEELVH"
BASE COUNT         1630 a         1333 c         1522 g         1755 t
ORIGIN      
        1 acaagcaagg gcagaacctc aaaaaatcgg ctctgcccag aataactaat gacatatctt
       61 atatcagttt tttgagttta cacaaaactt gaaacggtct cttttaaata cttttctgcg
      121 tcttcccttg tcaaaccaaa tttttcacat gtttcaatta agatggtttc tgtgggtatt
      181 tgcaactttt tcaaagtagc tatcattgcc tgaattcctt gctccagtcc tttttgcagt
      241 cctttctgaa tgccctgctc ctctacacct ttacttaaat tacacataag cgacacctca
      301 ctttctaatg tctgtgtcat ttttattttg aaatcttctt ctaatatctg gcatttgtcc
      361 tctgaagctg tttctgtcga aagaaggaca tttaacagct ttaacacatt tgtatctgtg
      421 gatgctttct ttccaagaca aatcatgaca gctgtcatta agtcataatt cgacttcttt
      481 tcttttacat ttcctatcag atttttctct gagatcacat aaccggtaat gctgttttca
      541 cggttcttcg gcggattaat tctcctgaca ccggagcagc tgccagaaaa cggatatcat
      601 aagtaatcgt gccctctgat aatgtggcat cctctacccc tgttccatga attctggaag
      661 tctgatttgt ttcatctggt gcaacggcag tttcacctac ttgcggctgt ccttcgatat
      721 agttttccgc tatttcattg acatcacaat ttttgtattc ctctacgcaa ttcttcatga
      781 tccatgctaa aatgattttt tctgataaaa gccgcttgca ggctgcatca tagcttgctt
      841 tttcatcagt tacacggata ttctttgcta ttgttgtgtc agtattcacg ccatcacctc
      901 ctgtagttat tgttattgta ccacaatatt ctttgagaaa ctatagaata tttgttaact
      961 gaaataaaaa gggggttatg aaaggaaaaa tattccggag acagatgctt tcggaaagga
     1021 acgaatgtca gatacactcg tttaaaaacg gaaggatgga atcaagcttt gtctttaaaa
     1081 tgccttccag ttcgatcact cgttcaagag tagcctgaac agaggtatca atggcaatga
     1141 gcgcctcgta gtcgctggaa gcggcaatta cccggttgat ttttgcgcat tctgcattta
     1201 aaatgcaggc cagagcgtct tcctcatggg cgatggagga tgcgatgctt aaaaggatgg
     1261 ggcaggggca ggcagtaggg cactgggtct ggcaggtcag agaagttgtt tcattcatag
     1321 tgattatctc ccttcttccc gatgatggat cgggatacta taatatatgg acaaggagaa
     1381 aagttgtgac agcctggccc aaaacagcta cagctccagg gttttagtgg cgattgtttc
     1441 acgaaaggcc cggtcgtgct ctacaaagat catagtaggg cgaaagtcta aaatcagctg
     1501 ttcaatctgc attctggaat aaatatcaat aaaatttaaa ggttcatccc acagatacag
     1561 atgggcctgt tcgcagagac tctttgcgat cagcaccttt tttttctgtc ctccggaaaa
     1621 ttgggccata tcctttgtaa actggatccg ctcaaagtcc agctttcgga gaatcgcttt
     1681 aaagaggctt tcatcaatcc tgcattggct ggcatagtcg gacagtgttc cgcttaaatg
     1741 ggaggtgtcc tgggaaacat aggagataat aaggccggag cctatagaaa tggttccggt
     1801 tttctttata tcttctccaa ggatcagctt taagagactg ctcttgccgc ttccgttttt
     1861 tccgtttaag gcaaggcgct ctccctgttc cagagtaaag gaaaacggcc cgcagacggc
     1921 acgatccccg tatcggatgg agaggtcaca gcaggaaatc agggtctggc ttcggtaacg
     1981 caggggcaga agcttaaggg cttctgctgt ttcagtgttt ttcagaagac cggatttttc
     2041 ctcaatggct ttttgctgcc tggactgaat ggatttggcg cgctgcatca ttttcgcaga
     2101 tttgtggcct acaaatcccc ggtctgcgca gccgcttccg attttggagg cttcgattcg
     2161 gtcggaccag ccggcagtac gggcagatgc ctgctttaga cggccgattt cctttttgag
     2221 ctgttcattc tgggccagct cacaggcttg cctgtatgta aaattagtca tccaggagga
     2281 aaaattgccg ctctgtacct cgatatcagc ccggtttaac gaaagaatgt gatccacgca
     2341 gctgtccaaa aagaagcggt catgggaaac caggataaag ccctttttct tcttgaggta
     2401 agctgcgacg gtttctctgg cagctgcatc caggtggttg gtaggctcgt cgatcagaag
     2461 aaaatggccc tgatttaaga aaagtgcagc gaggagcgct tttgtctgtt cgcctttgga
     2521 cagagtggaa aatggcctcc agagcagttc gcagtccgtc tgcagacagg agagttcctt
     2581 cataaattcc cagtcctgag catcgggaca gatttcatac atgatttcca gtgtcatttt
     2641 agagggatct gaaacctcat aggggaaata gtcaaaggag acagaagaat ggatggttcc
     2701 ccggtattca tattttccca tgagcagatt taaaaatgtg gttttccccc tgccgtttct
     2761 gcctacaaag cccagcttcc agtcggtatc gatctgaaaa ttcacatctt caaaaatggg
     2821 gtcggagctg gatgggtagg aaaaggtaag atgttccgct tttatcattg acataatgtg
     2881 cacctccggt aaaaaaatga gagccgcagg aaagcagatc ctgcggctca tcataaaatt
     2941 acccctgaaa gacagaggcg tatattttag tgagtggaca gatatacgac tttcttgcag
     3001 cggacgcacg gataccgtaa ataaaatgcg gtattctggc gtcattttat agttatctgc
     3061 aagcgtcgat tctcatcctt tcacctcttt ctgttctggt atagatttat gtatatcata
     3121 gatcagggga agatgcaaga ggaataggta tcatttacaa ggcgatcctt atcattaatt
     3181 ctgtggctga attcaccctg taagtcgcct gtcagcggtt cgggtttacc gattccctcc
     3241 atacaaccat ttcgttcaat atctttaata agctgattga tgcgccggat ggtttttttt
     3301 gtcctgagat tgccagtaaa ggtaatcttc ccaggcatca tcagaccaga ttttttcact
     3361 catcatctac ctcaatcagt tcgtgggcag taccttttcc tgtgcgtagt tcctgaatgg
     3421 atttttttac atattccaga ttagactggg agaagaatgg atccgaactt tgagcgattt
     3481 caaatgggat tcggttttcg cgtaaaactg ctttcacaaa aagattgatg gcagttgagg
     3541 tgtttaaacc tacgttggaa caaaaggcat cgaagttgat tttatctttt tcatctaccc
     3601 gcgcggttaa agttgcctgt gccatagata taactccttt ctgtgttcta tttgttataa
     3661 ttataacact attgtatgca aatagcaagt aaatgcaata caaaataaaa ttgtgtgaaa
     3721 ggcagacatg atattcgccg gaattgccat tgaaaattgc ttcggtatct gtataatatt
     3781 aggaagatag tacgcaggat aatacgcaag ttactgttca acaggaggac aaacacatgg
     3841 aacgctcaaa gctgacgact ctctgctaca tggagcggga gggagaatgg ctgatgcttc
     3901 acagagtgag caagaagaat gatgtaaata aagacaaatg gatcggcatt ggaggccatt
     3961 ttgaagaaaa tgaaagccct gaggaatgcc tgttgaggga ggtaaaggag gaaacgggcc
     4021 tgacacttac ttcctggcgg ttccgggggc ttgttacatt ccgttctacc ggaggtccgg
     4081 tggaggatat ggagtatatg tgtctgtaca cggcagacgg ttttgagggt gagatggctg
     4141 actgcaatga gggtactctg gaatgggtaa agaaggaggc agtgtgcgga ctgaatatct
     4201 gggagggaga taagatattt ttccgccttt taaatgaaaa tgcccccttc ttttcactga
     4261 agctgaccta cgaaggcgac cggcttattg aggctgtgct ggatgggaaa gcgcttatcg
     4321 cttaatgcac aagctcctca gcccgtttga tcgccctgtc aatattttca cagaagtttt
     4381 cctggcctac cttctgagca aagcctgctt tttccattac cttcatgggc tgctggttta
     4441 catgggagag aataagggtg attccccgtt tctcacagtt tttgtacagg tcggtcatgg
     4501 cgtttaatgc agtgctgtcc agagccggaa cgcttctcat acgcaggatc aggcattttg
     4561 catgatcttt tacggcaata cgtccaatca catcggcggc tccgaagaac agggggccgc
     4621 tgatttcata tacattgatg tgaggggaca gctttctaag tttttcgcgt tctgcctcct
     4681 cttcatcctc tgcatatttc cagctgtaga cctccgttac atcgctcatg cgcttcataa
     4741 acagaagcac agccactaag aggccgattt cgatagcgat taccaggtca aagacaatgg
     4801 ttaagaggaa ggtaaggatc agaacggcga tatcgctttt gggagcggtt tttaccagct
     4861 ctgtgaaggt tctccactgg cacatattgt aggcgaccat aaagaggata gcggcaatgg
     4921 cgggcatggg gatcatggct gcatatggca tgagtataat caggataaga acaagaacca
     4981 cagcgtggat catgcctgca acgggagtac ggcctccgtt ttttacattg gccgcggtac
     5041 gggcgatggc gcctgtagcc gggatgccgc caaaaagggc ggaagcgata tttccggcgc
     5101 cctgggccgc cagttccata ttagaacggt gttttccatt gatcatgccg tctgctacca
     5161 cacaggagag cagagactca atggccgcca ggatagcgat ggtaaaagcg tcaggaaggg
     5221 cagtctggat catttcaaag cttaactgag gcatatggaa ggaaggaagc ttattgctta
     5281 tggtgtagag atctccgatg gtattgacct gaagggggag aagctgcacc atgagaatgc
     5341 ctccgatgac ggcaagaaga gagccgggga ttttttccgt aaaccggggt gcgatgataa
     5401 gaatagccag gcatacgagg cccacgatca gcgagtctgt attgaatgta tggatgcctg
     5461 ctaagaaggc tttcagcttt tccatggttt caatggtggg cattccttcc ggataggtta
     5521 cgccgaaaaa gtccttgagc tgtccgatga cgatggttac ggcaataccg gaggtaaagc
     5581 cggttgtgat ggtaaatggg atgtacttaa tcagcccgcc gaaccggcag agtcccatga
     5641 tgattaaaat gatgcccgca aggatggtag cggctgccag tccgtccatg ccgtttcggg
     5701 ctacaatgcc cgctacgatg gtggcgaagg ctgctgtggg gccggcgatc tgaacctggc
     5761 ttccgcccag gaatgagatg ataaagccgg ctacaatggc agtgtagata ccctgttccg
     5821 ggcctacgcc ggaggcgagc gccagggcga tggacagcgg aagggcaatg atggcaacta
     5881 cgatgcctgc gatgaggtct tttaaaaatt gttctttgct gtaggttttc catgttgtaa
     5941 acagcatggg ataaattttt tccataatca gttccccttt ttcatattga taggcaggaa
     6001 aatgctccct gccctacctt attatacagg cgcaggatgg tatgtccagc aaaaaggcag
     6061 aaaatattgt attttttgag gaactgtata tttcacagaa tcctgaggat actttataag
     6121 gcagcttaca gacacccatt cataaaggag gaactaccat gaaagtaaac ggaacgaacg
     6181 aatgtatcca gtgtaccatc aagaattgtg cgtatcatgc agataccacc gattactgta
//
LOCUS       DBMDUH010000155         6117 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_5213_length_6117_cov_4.344249, whole genome shotgun sequence.
ACCESSION   DBMDUH010000155 DBMDUH010000000
VERSION     DBMDUH010000155.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 6117)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6117)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6117
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5213_length_6117_cov_4.344249"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1035
                     /gene="eno"
                     /locus_tag="ACZPCG_11200"
     CDS             <1..1035
                     /gene="eno"
                     /locus_tag="ACZPCG_11200"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HIR1285657.1"
                     /translation="MDPCNIYEVDHAMIKADGTADRSKLGANAILAVSMASAAAAANA
                     LGLPLYRFLGGVNAGCMPLPMMNIINGGAHAANTVDVQEFMIMPAGAESFREGLRWCA
                     EVFHALAAFLREKGLATAVGDEGGFAPDLESDEEAILCILEAVKRAGYEPGRDFVLAL
                     DAAASEWKGERTGEYILPKCGKKFTSSELTEHWKKLCETYPIYSIEDGLDEEDWEGWK
                     YMTKELGNRIQLVGDDLFVTNTKRLKKGIGWGCGNSILIKPNQIGTISETLEAVRLAR
                     RSGYTAVISHRSGETEDTAIADLAVALNTGQIKTGAPSRSERVAKYNRLLRIEEELGE
                     RAEFLGKGAL"
     gene            complement(1030..3183)
                     /locus_tag="ACZPCG_11205"
     CDS             complement(1030..3183)
                     /locus_tag="ACZPCG_11205"
                     /inference="COORDINATES: protein motif:HMM:NF024242.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1285658.1"
                     /translation="MLISAAIFYHTSYHHLKKGLHSQTVSVLSSSVRQMDDLLKQIHT
                     ASLQLSSSSSFKALADSENMETESFSYSAWRVQEQLKLILPSKELNTGGQLFIYLKNS
                     GYILSSNFFSELELARKHDSRYTFSVDLMSPSAWRTFIPVNEGDSFLYICPVSSSLIS
                     INGTVNSVLCFMVDRNSMERLFPEIRLSDDYQICALDKDGSPCFSIRGSASPEIDLQL
                     LFGLSYSDKTADFKDKGRSISAVQTVSDYNGWNWYLIQPSSTLYYSAENYQRLSIVLI
                     ILTVLLESGFIVLLTAYNSRSVADLSSELENQHHLASALTTMVEQAKPLVSESYIRKV
                     MEGNITTNEQMERITSELGLKREGCRYQVLYIRAAPRHAHNLDAEALKLCIENHDILV
                     QEALKRYFPDTGHIYKPGDAAFACLIAVPSARSDNENTEQNIQIFRSVHQELLNLYDI
                     EIRGGLGNICEVVPYIWKSYQEAHNAWSMTTDQRCIISSLRLDSSTDTYYFPESLAVQ
                     LSGFISTGSSSQTEALFSCLKEENLEKRTLSFTQLRWLVSDIRTVLFRKRLSVDPSLT
                     DAPERKELLDLIDRQFENEISLDSLQSIALQLCSFYGSCGDSNELIQKIQNYLNRNYM
                     DSSLSLSKISEEFHISENYFSFLFKKEVSENFSVYLEKLRMAKAKELVLESSISISEL
                     YQYTGYNNAASFRRAFKKKFGVSPKEMREKAASGL"
     gene            3542..5107
                     /locus_tag="ACZPCG_11210"
     CDS             3542..5107
                     /locus_tag="ACZPCG_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013282391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR1285659.1"
                     /translation="MNKRVRCTAAFAAAVMAASLLGGCGQNGVTAPENVEKYTADGEL
                     IKPEKITVMADGTVFTKRNGRDAFEARWEELTGIDIEFIQPDHSAYYDVVGQTFASGV
                     KNWPDVILLSSTYYSGYAEEGALWDLTETYENSDLKKSGRLTGEDVIDGMRIGGRLYG
                     LPVNRGGGCITYVKKKWLDNCNLEVPTNYDEYMNMLKAFTEGDPDGNGVNGDTYALSA
                     AGFMGEESPYTNYLPEFYQESYPSFTKDENGVWYDGFMEQEFKDSLLRLRDAYVNGYV
                     DKETLTNGTSDVRNKFYEDRCGVFTYWSGTWAENLRSNLAKNGLDDELVPLPPIKELG
                     NYIERTPAVWCITNSCKYPEAVYKYFIESMMDGGDMQTLWTYGVEGVHWSVQAETVCG
                     NTYEQGQFHGLESMDKPGTQYTKGHMDPTLSIVEWENDPGIDSVAPAAKRSQEIFNEN
                     CKQSLMVPSTTEMATYNGDLTTLKRSIVADVVVQGMPVEEAYQRFEKEHGVKWSNMIV
                     DSLNRLAEAESSR"
     gene            5135..6088
                     /locus_tag="ACZPCG_11215"
     CDS             5135..6088
                     /locus_tag="ACZPCG_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1285660.1"
                     /translation="MKKETSNPGTGTVHKKSLKQQIYYYRSFYIMFIPVFIFAVVFHY
                     LPMLGIRYSLFSYKGIKAPEFIGLANFERMFSMPGFWSAFGNTILLSVVKLLLNTFMA
                     VLISILLNELRFMKFKKVTQTIVYLPHFMSWVVTASVFTLILSPGDAGLVNSILIKLG
                     IIDEGIYFLGDKEWWRTMFYAINIWKDTGWGTIIFMATLSGINPELYEAASMDGANRW
                     NQIRYITLPALANTIITVLILNLAKVMNLFESVFVLQNDAVIQKADVLQTYIYTQTFN
                     SGALPDFGYTTAVSVTASVVGCILVLLCNRASYKVRGRGIV"
BASE COUNT         1733 a         1226 c         1620 g         1538 t
ORIGIN      
        1 atggatccct gtaatattta tgaggtggat catgccatga tcaaggcaga cggcacagca
       61 gaccggtcaa agctgggagc caatgccatt ttggcggtat ctatggccag tgcagcggca
      121 gcggccaatg ccctgggact tccgctgtac cgttttctgg gaggcgtcaa tgcgggctgc
      181 atgccgcttc ccatgatgaa tattataaac ggaggagccc atgcggcaaa tacggttgac
      241 gtacaggaat ttatgatcat gcccgcagga gcagaaagct tcagggaagg actgcgctgg
      301 tgcgcggagg tgtttcatgc cttagccgca ttcttaagag aaaaggggct ggctactgct
      361 gtaggcgacg aagggggatt tgctccggat cttgaaagcg atgaggaagc aatcctctgt
      421 attttagagg cggtaaaacg ggccggatac gagccgggaa gggattttgt gcttgctttg
      481 gatgcggcgg ccagcgaatg gaaaggggaa aggacgggag aatatattct tcccaagtgc
      541 ggaaaaaagt ttacctcttc agagctgaca gagcactgga agaaattgtg tgaaacgtat
      601 cccatatatt ccattgagga tggtcttgat gaagaggact gggaaggatg gaagtatatg
      661 acaaaggaac tgggaaacag gatccagctg gtaggcgatg atctttttgt taccaataca
      721 aagcggctga aaaagggtat tggatgggga tgcggtaatt ccattttgat caagcccaat
      781 cagatcggaa cgatttcaga aacgctggaa gcggtccggc tggcccgccg gtccgggtac
      841 acggcggtta tttcccaccg ctccggagaa acggaggata cagcgatcgc cgatctggca
      901 gttgccctga atacgggaca gatcaaaaca ggggctccca gccggagcga acgggtggca
      961 aaatacaacc gtcttttaag gattgaagaa gagctgggag aaagagcaga gtttttggga
     1021 aagggcgctt tatagaccgg aggctgcttt ttcacgcatt tcttttggtg atacgccgaa
     1081 ttttttctta aatgcccggc ggaaggaagc cgcattgttg tagcccgtat actggtacag
     1141 ctcagaaatg gaaatcgagg attccagtac cagttccttt gcctttgcca tccgcagttt
     1201 ttccagatat acggaaaaat tttcagaaac ttctttttta aaaagaaagc tgaagtaatt
     1261 ttccgaaata tggaattcct cagagatttt agaaagagat aaggaggagt ccatataatt
     1321 acgattcaga taattctgga ttttctggat cagttcattg gagtcgccgc aagagccata
     1381 aaagctgcac agctgcagtg cgatggactg cagggaatcc aggctgatct cattttcaaa
     1441 ctgacggtcg atcagatcca acagctcctt tcgttccgga gcatctgtta aggatgggtc
     1501 aacagacagg cgttttctga aaagaaccgt tcttatatcg gaaacaagcc agcgaagctg
     1561 tgtaaaggaa agggtgcgtt tctccaggtt ttcttccttt aaacaggaaa aaagagcttc
     1621 tgtctgagaa ctgcttccgg tagaaataaa gccgcttagc tgtacggcaa gactttcagg
     1681 aaaatagtat gtatcggtgg aggaatcaag acgcaggctg ctgatgatac agcgctgatc
     1741 ggtagtcatg gaccatgcgt tgtgggcttc ctggtatgat ttccagatat agggaactac
     1801 ttcacagata tttcccagac cgcccctgat ctcaatatca taaagattta aaagctcctg
     1861 atgaaccgaa cggaatatct gtatattctg ttcggtattt tcattgtccg aacgagcgga
     1921 gggaacggca attaaacagg caaaggcagc atcgccgggt ttatagatat gtccggtgtc
     1981 gggaaagtag cgcttcagcg cttcctgtac caggatatca tggttttcaa tacacagctt
     2041 tagggcttct gcatccaggt tatgggcatg gcggggagct gcccttatat aaagaacctg
     2101 atagcggcat ccttcccgtt ttaagcccag ttcagaggtg atccgctcca tctgttcatt
     2161 cgttgtaata ttaccctcca tcactttacg gatataggat tccgatacaa gaggctttgc
     2221 ctgttctacc atagtagtca gggcagaagc cagatgatgc tgattttcca gttcactgct
     2281 cagatcggca acggatctgc tgttataagc cgttaaaagg acgataaagc cactttctaa
     2341 cagtacggtc agaatgatca gtacaatcga aagccgctgg tagttttcag cagaataata
     2401 aagggtagaa gagggctgaa tgagatacca gttccagccg ttatagtccg atactgtctg
     2461 tactgcgctt atggaacgcc ccttgtcttt aaaatcagcc gttttatccg aataggaaag
     2521 accgaaaaga agctgcaggt caatctcagg agaggcagag ccgcggatgg aaaaacaagg
     2581 gctgccatct ttatccaatg cacagatctg atagtcgtct gacaggcgga tttcaggaaa
     2641 aaggcgttcc atgctgtttc gatcaaccat aaagcagagg acgctgttta ccgttccatt
     2701 tatggaaata agagagctgc ttacagggca gatatagaga aagctatctc cttcattgac
     2761 cggaataaat gttctccatg cagacgggga cattaagtcg acggaaaatg tgtagcggga
     2821 atcatgcttt ctggcaagct ccagttcgga aaagaagttg gaggataaga tatatccgct
     2881 gtttttcaga tagataaaca gctgtccgcc tgtattcagc tcctttgaag ggagaatcag
     2941 tttaagctgc tcttgaaccc tccatgcgga gtaagaaaag gattccgttt ccatattctc
     3001 agaatctgcc agggctttaa aagaagaact ggaagaaagc tgcaggctgg cagtatggat
     3061 ctgttttaaa agatcatcca tctgacggac agaggatgag agtacgctga ctgtctggga
     3121 atggagccct ttttttaaat gatggtagga tgtatgataa aaaatagctg cgctgatcag
     3181 catcagggcc agaataaaac tgtatgacag tgcaagcctg tttttaaagg atatgttttt
     3241 tagtgttttc ataggtgcca ttataatata agaacgggag agaatcaact gaaaaagaga
     3301 aaaatgtatg ttatccgtaa aaaatgtgtg ttttgcagtg cagatcttaa tggaaacgtg
     3361 aaaaaacttt gaatgctata aagaaaaaga aatagacgta aaaacaaaga atttcatgaa
     3421 aaatgcacaa tgaaatgaaa ttttaacagt tgatatgaag cagtaagagt gataattttg
     3481 tagacacata aaaacgtgtg gtgaaatcag catacgaaat ctatgatctg gagggaaaac
     3541 catgaacaaa agggttaggt gtaccgcagc cttcgctgca gcggtcatgg cagcgtctct
     3601 gcttggcggc tgtggtcaga atggcgtgac agcacctgaa aatgtggaga agtacacagc
     3661 agacggagaa ctgatcaaac cggaaaaaat tacggttatg gcagacggta cggtttttac
     3721 gaaacggaat ggacgcgatg cctttgaggc gcgctgggaa gagctgacag ggattgatat
     3781 tgaatttatc cagccggatc acagcgctta ttacgacgta gtaggacaga cattcgcttc
     3841 tggcgttaaa aactggccgg atgtgatcct gttaagttct acctattaca gcggctatgc
     3901 ggaggaaggt gctctgtggg atttgacaga gacgtatgaa aattctgatt taaagaagtc
     3961 cgggcggctg acaggagaag atgtcattga cggaatgcgc atcggcggac ggctttacgg
     4021 acttcctgta aaccgcggcg gcggatgtat tacctatgta aaaaagaagt ggctggacaa
     4081 ctgtaatctg gaagtgccga caaactatga cgaatacatg aatatgttaa aagcatttac
     4141 agagggagat ccggacggaa acggtgtgaa cggcgataca tacgctcttt ccgcagccgg
     4201 ctttatgggt gaggaatctc cttataccaa ctacctgccg gaattttatc aggaatccta
     4261 tccttccttt acaaaggatg agaacggagt gtggtatgac ggctttatgg agcaggagtt
     4321 taaggattcg ctgcttcgtt taagagatgc ttatgtaaac ggatatgttg ataaggaaac
     4381 gctgaccaac ggcaccagcg atgtgcgcaa caagttttac gaagaccgct gcggcgtatt
     4441 tacctactgg tccggaacct gggcggaaaa cctgcggagc aatctggcaa agaatggact
     4501 ggatgacgag ctggttcccc ttccgccgat caaggagctg ggaaattata tagagagaac
     4561 gccggctgtc tggtgtatta ccaattcctg taaatatccg gaggcagtgt acaaatattt
     4621 tattgaatcc atgatggacg gcggcgatat gcagacgctc tggacatatg gcgtagaggg
     4681 cgtgcactgg tctgttcagg ctgagacggt ctgcggaaac acctatgagc aaggccagtt
     4741 ccatggtctg gaaagtatgg ataagccggg aacccagtac acaaagggac atatggatcc
     4801 gacgctttcc attgtagagt gggaaaatga tcccggtatt gactccgttg ctccggctgc
     4861 caagcgttca caggagatat ttaatgaaaa ctgcaaacag tcccttatgg ttccctccac
     4921 aacggaaatg gctacctata acggcgatct gacaacctta aagcgttcca ttgtggcaga
     4981 tgtagtcgtt cagggaatgc cggtagagga ggcataccag aggtttgaaa aggagcacgg
     5041 tgtaaagtgg tctaatatga tcgtggattc ccttaaccgc ctggcggaag cagagtcatc
     5101 caggtaaaca aaagagaaat gggaggaaaa tagaatgaaa aaagaaacat caaacccggg
     5161 aacgggaacg gtgcataaaa aatctctgaa gcagcagatc tattattaca gaagcttcta
     5221 tattatgttt atccctgtat ttatttttgc agtcgtattt cactatctgc caatgctggg
     5281 aatccgttat tccctgtttt cctataaggg cattaaggcg ccggaattta tcggtctggc
     5341 aaactttgag cgtatgtttt ccatgccggg cttctggagc gcgtttggaa atacgatcct
     5401 gttaagcgta gtaaagcttc ttctgaatac gtttatggcc gtgctgatct ccattctgct
     5461 caacgagctg cggtttatga agtttaaaaa ggtaacccaa accattgtgt atctgcccca
     5521 ttttatgtca tgggttgtaa ctgcctctgt atttaccctg atcctgtctc ccggtgatgc
     5581 aggactggta aattcaatcc tgatcaagct gggcattatt gacgagggaa tctatttcct
     5641 gggagataag gaatggtgga ggaccatgtt ctatgcgatc aatatctgga aggatacggg
     5701 ctggggaacc atcattttca tggctacctt atcgggcatc aatccggagc tttatgaggc
     5761 tgcttccatg gacggagcaa accgctggaa tcagatccgc tacattacgc tgccggctct
     5821 tgccaatacc attattacgg ttttgatctt aaatcttgcc aaggttatga atctgtttga
     5881 atcggtattc gtactgcaga atgatgcggt gatccagaag gcggacgttc ttcagaccta
     5941 tatctatacc cagaccttta attcaggcgc gcttccggat ttcggttata ccacagcggt
     6001 cagcgtgacg gcgtcggttg taggctgtat tctggtgctg ctgtgcaacc gggcaagcta
     6061 taaggtccgc ggcagaggca ttgtatagga ggaataaaaa ttatgagaac gaaaaga
//
LOCUS       DBMDUH010000156         6115 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_5217_length_6115_cov_6.099268, whole genome shotgun sequence.
ACCESSION   DBMDUH010000156 DBMDUH010000000
VERSION     DBMDUH010000156.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 6115)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6115)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6115
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5217_length_6115_cov_6.099268"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            114..869
                     /locus_tag="ACZPCG_11220"
     CDS             114..869
                     /locus_tag="ACZPCG_11220"
                     /inference="COORDINATES: protein motif:HMM:NF013539.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HIR1285661.1"
                     /translation="MNLDLNKLTCGVSLTDTEQAVLEYLLLHLDEALKLGVRGVAKAN
                     FTSTSTVMRLSRKLGYNGFIEMYYKLLGAVGEGSQSYELNEGFISHFAGEGAVSMENY
                     RSLRLAAEKIYQTDQVVFIYGMGFSSMMAEYLAKKLLVLGIKCIFSDGADSIGVFENN
                     LEDIGVFIVFSRSGRSPYVLNRVKMAEENTVFTVGFTNDGASPLKEHCGCIIEVPDDN
                     PLDDRNMKPTLFFSKIMTMIELLVYEYYQVSLG"
     gene            999..2582
                     /locus_tag="ACZPCG_11225"
     CDS             999..2582
                     /locus_tag="ACZPCG_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HIR1285662.1"
                     /translation="MKEKVMSTMQAFSKAMFMPVLILPIAGLLIAVGNVFTNARLIEI
                     LPFLDNPVTKGFGTILSGALVAILNNLGLIFCAGIAVGLAKKKKAEAGFTAMLAFLVF
                     INAMNKFMDLRGILADPAALRGTGQAMVLGIQILDMGVFLGMILGCVTAYVHNRFIDT
                     EFNNAFQIYGGTRFVFIVLIPVVILLAVVLTYVWPVVQSGIDQLGYFINQAGNFGIFL
                     YGALERLLIPTGLHHLVYTPFLYTNLGGTAEVAGQVFEGCRNIYYAEMADPNVAMLSA
                     SVVWDARGLSKMFGLVGACLAMYHTARPENRTKIKAILIPAAFTSFIAGVTEPIEFSF
                     LFTAPILFVAHAVLSGLGMVVFNLLNCRAIGPNGFIDFVLYNLPLGIEKTAWPTYLLI
                     GVGEFVVYYLLFRFMIAKFNLKTLGRESEGMEMKLHTKDEYKEKQEGKKNAKGEEINA
                     ALIVEALGGAGNIVKVDNCFTRLRLIVKDNTLVQDDVLKQKTGAAGVMKKGENVQVIY
                     GLHINQVRQAVDRELGLDQ"
     gene            2607..3941
                     /locus_tag="ACZPCG_11230"
     CDS             2607..3941
                     /locus_tag="ACZPCG_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017212113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HIR1285663.1"
                     /translation="MAKKAFDVVIAGGGSTYTPGIVKMMLEHQEDFPLNSVTLYDNDG
                     ERQAVIGEALEILMKEEAPHIHFSYTTDPAEAFTGKDFCMAHIRVGKYAMRQLDERIP
                     LKYGVVGQETCGPGGIAYGMRSITGMLEIIDYMVKYSPECWMLNYSNPAAIVAEACRV
                     LRPDARVINICDMPVGTRRRMAYIVGKEYDDLDVRYFGLNHFGWWTSVKDKTTGEDYM
                     PKLLEYVSENGYLTQKAIETQHMDESWQATHRKAKDLLALDPQFLPNTYLKYYLYPDY
                     VVEHSDPNHTRADEVIEGREKEVFGAAREIIRKGTATGGEFTVDNHASFIVSLAKAIA
                     YNTHEIMLCIVENKGAISNFDSDAMVEVPCVVGSNGPEAMCQGEIPTFERGMMQEQLA
                     VEKLVVEAYVEKSYLKLWQALTLSKTVPSAGVAKQILDELIVANKEYWPELK"
     gene            complement(4023..5069)
                     /locus_tag="ACZPCG_11235"
     CDS             complement(4023..5069)
                     /locus_tag="ACZPCG_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005427582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HIR1285664.1"
                     /translation="MTFYQELQLNSAGSKALIRITRDKKERMRHILIYNFKVYLVVAF
                     CFAAVTLFSMIFGNENSIAGVVVLLALLVLRQADFGIHTSHGLLCIAGIFGILMTGPR
                     LSNMVSPFAAFFINLICILLLMLLGCHNVIMSNHSTFVLSYLILQGYDVSGYSWMMRT
                     AGLFVGMLLCMAVFYKNQKHRLHKREFKDLFHEFDPRSLRNRWYIKLTLCVSTVLLIA
                     SLLGIPRAMWAGIACMSVCLPFQKDLVKRAHIRWIYNILGCMIFLVIYAVLPKELHPM
                     IGLLGGIGVGYSAGYPWQTVFNTFGALSIASGLFGAAGAVILRIATNAGAAFYTALMG
                     SLIDRASDRLFCRE"
     gene            complement(5266..6115)
                     /locus_tag="ACZPCG_11240"
                     /pseudo
     CDS             complement(5266..6115)
                     /locus_tag="ACZPCG_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604671.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
BASE COUNT         1635 a         1294 c         1537 g         1649 t
ORIGIN      
        1 ggtttgtgag cagatctggt acagctaccg ggatggcagg tatattgacc tgatcgaccg
       61 gatcgtggca gttggatatc tgcagaagct gcccgagcag gaaggagaaa tttatgaatc
      121 tggatctgaa taagctgacc tgcggtgtgt ctttgacgga tacagagcag gcggtgctgg
      181 aatatctgct gcttcatctt gatgaggctt tgaagctggg ggtccgggga gttgcaaagg
      241 ctaattttac ttctacttct acggtgatgc gtctttccag aaagctgggc tataacggtt
      301 ttattgagat gtattataag cttttggggg ctgtgggaga aggaagccag agctatgagc
      361 tgaacgaggg ctttatcagc cattttgcag gagagggagc tgtttccatg gaaaattacc
      421 gcagcctccg tctggctgca gaaaagatct accagacaga tcaggtggtt tttatctatg
      481 gcatgggatt ttcatccatg atggcagaat atctggcaaa aaagctgctg gtattgggaa
      541 tcaagtgcat tttttctgat ggggctgatt ccattggagt ctttgagaat aatctggagg
      601 atatcggcgt ttttatcgtt ttttccagat cgggccgttc tccctatgtg ctcaaccgtg
      661 tgaaaatggc agaggagaac acagtgttta ccgtgggctt taccaatgac ggggcaagcc
      721 ccttaaagga gcactgcggc tgtattatag aggtgccgga tgacaatcct ttagatgaca
      781 gaaacatgaa gccgaccctg tttttttcta agataatgac catgatcgag cttttggttt
      841 atgaatatta ccaggtttct ctgggataag aaaacgtgtt acacatccgg gtaacacgtt
      901 ttcttttgtc tgaaatctat gtacttttgc ccgttgtctg cgtataatcc aatcatcgac
      961 cacgcggggt taaagttaga aaaacggaga ggtatgaaat gaaagaaaaa gttatgagca
     1021 caatgcaggc attttccaaa gctatgttca tgccggtgct gatccttccg atcgccggtc
     1081 ttctcattgc agtgggaaat gtatttacaa acgcaagact gatcgagatc cttccattcc
     1141 tggataatcc ggtaacaaag ggcttcggaa ccatcttatc cggcgctctg gtggctattt
     1201 taaataacct gggactgatc ttctgtgcag gaatcgcagt tggactggca aagaagaaaa
     1261 aggcggaagc cggatttact gccatgctgg catttctggt atttatcaat gccatgaaca
     1321 aatttatgga tcttcgcggt attttagctg atccggcagc cttaagagga accggacagg
     1381 cgatggttct gggaatccag attctggata tgggcgtatt cctgggaatg atcctgggct
     1441 gcgtgaccgc gtatgtacac aaccgtttta ttgatacaga atttaacaat gcgttccaga
     1501 tttacggcgg aacacgtttc gtatttatcg ttctgattcc tgtggttatc ctgttggcag
     1561 tagtgctgac ttatgtatgg ccggtggttc agtccggtat tgaccagctg ggatatttca
     1621 tcaaccaggc tggaaacttc ggaatcttcc tttatggagc attagagcgt ctgctgatcc
     1681 ccacaggact tcaccatctg gtatacactc cgttcctgta caccaatctt ggcggtactg
     1741 ctgaggtggc aggacaggta tttgagggct gccgcaacat ttactatgca gaaatggcag
     1801 atccgaacgt agcgatgtta tctgcttctg ttgtatggga tgcaagagga ctttccaaga
     1861 tgttcggtct ggtgggcgca tgtctggcta tgtaccatac tgcaagaccg gagaaccgta
     1921 caaagatcaa ggcaatcctg atcccggcgg catttacctc ctttatcgca ggcgtaacag
     1981 agcccatcga gttctctttc ctgtttacag cgcctatcct gtttgtagcg cacgctgtat
     2041 taagcggttt gggtatggtt gtatttaacc tgctgaactg ccgtgccatc ggaccgaatg
     2101 gttttattga ctttgtcctt tacaacctgc cgttggggat tgaaaagaca gcatggccga
     2161 cgtacctgct gatcggtgta ggcgaatttg tagtttacta cctgctgttc cgttttatga
     2221 tcgccaagtt caacttaaaa actctgggac gtgagtctga gggcatggaa atgaagcttc
     2281 ataccaagga tgagtacaag gagaagcagg aaggcaaaaa gaacgcaaag ggcgaagaaa
     2341 tcaatgcagc tctgatcgtg gaagccctgg gcggagcagg caatatcgta aaggtagaca
     2401 actgctttac ccgtctgcgt ctgattgtaa aggataatac tctggtacag gatgatgttt
     2461 taaagcagaa gacaggagct gccggagtga tgaaaaaggg agaaaacgta caggtaattt
     2521 acggtctcca cattaaccag gtgagacagg cagtggatag ggaactgggc ctggatcagt
     2581 aaagtttaga aagggagtat gcagatatgg caaagaaagc atttgatgta gtaatcgcag
     2641 gtggcggaag cacctataca ccgggaattg tgaaaatgat gctggagcac caggaggatt
     2701 ttccgttaaa ttccgttacc ctttacgaca atgacgggga gcgtcaggct gtcatcgggg
     2761 aagcgctgga gatactgatg aaggaggaag caccccacat tcatttctcc tacaccacag
     2821 atcctgcgga agcatttaca ggaaaggatt tctgtatggc acatatccgt gtgggaaaat
     2881 acgccatgcg ccagttagat gagagaatcc ccttaaagta tggtgtggtt ggtcaggaaa
     2941 cctgcggacc gggcggtatt gcctacggta tgagaagcat caccggtatg ctggagatca
     3001 ttgactatat ggtaaaatat tctcctgagt gctggatgtt aaactactcc aatccggccg
     3061 ctatcgtggc agaggcctgc cgggtactgc gtcccgatgc aagagtcatc aatatctgcg
     3121 atatgcccgt aggaaccaga agaagaatgg cctacattgt gggaaaagag tatgatgatc
     3181 tggatgtgcg ctatttcggc ctgaaccatt tcggctggtg gaccagcgta aaggataaga
     3241 ccacaggcga ggattatatg ccaaagctct tagagtatgt gtctgaaaac ggttacctta
     3301 ctcagaaggc cattgagact cagcacatgg atgagagctg gcaggcaacc cacagaaagg
     3361 caaaggacct tctggcactg gatccccagt ttttaccaaa tacttatctg aaatattatc
     3421 tttatcccga ctatgtggta gaacattccg acccaaacca cacccgcgcc gacgaggtga
     3481 tcgaaggccg tgagaaagag gtattcggcg cagccagaga gatcatccgc aagggtacgg
     3541 ccaccggcgg cgagtttacc gtggacaatc acgcttcctt tatcgtgtct ctggcaaagg
     3601 ctatcgccta caacacccat gagatcatgc tgtgcatcgt ggagaacaag ggcgcgatca
     3661 gcaactttga ctccgacgcc atggtagagg ttccctgtgt agtaggaagt aacggtcctg
     3721 aggctatgtg ccagggcgag atccccacct ttgagcgcgg gatgatgcag gagcagctgg
     3781 cagttgagaa gcttgtggta gaggcttatg tagagaagag ctacttaaag ctgtggcagg
     3841 ctcttactct gtccaagacc gtgccaagcg ccggtgtggc aaagcagatc ctggatgagc
     3901 ttattgtggc aaataaggag tattggccgg agcttaagta atattttaaa atgaaaaaca
     3961 gctcagggtg tgctgcctga aaatggcggc ggcccttggg ctgttttttg attttccata
     4021 ttctattccc tgcagaaaag ccggtcggaa gccctgtcaa tgagactgcc catcagagct
     4081 gtatagaagg cagcgcctgc attggttgca atgcgcagga tcacagcgcc ggccgcaccg
     4141 aaaagtccgg aggcaatgga gagggcgccg aaggtattaa ataccgtctg ccaggggtat
     4201 ccggcagagt atcccactcc gatgccgcct aaaagtccga tcatgggatg gagttcttta
     4261 ggcagaacgg catagattac aagaaagatc atgcatccca ggatgttgta gatccagcgt
     4321 atgtgagccc gttttacaag atccttctgg aagggaaggc acacggacat acaggcaatt
     4381 ccggcccaca tggcccgtgg gataccgaga agggatgcaa ttaaaagtac cgtagataca
     4441 cataaggtca gcttgatata ccaccgattt ctcagggagc ggggatcaaa ttcgtggaac
     4501 agatccttaa attctctttt gtgaagccgg tgtttctgat ttttataaaa tactgccata
     4561 cataaaagca tacctacaaa aagcccggcc gtacgcatca tccaggaata gccgcttaca
     4621 tcatagccct gaagaatcag ataggacagc acaaaggtag aatggttgga catgattaca
     4681 ttgtggcagc ctaaaagcat caaaagcagg atgcagatca gattgataaa aaaggcggca
     4741 aagggagata ccatattgga cagtctgggg ccggtcatga gaatgccgaa gatgccggca
     4801 atacataaaa gcccgtgaga ggtatggatt ccaaaatcgg cctggcgcag cactaaaaga
     4861 gccagcagca caactactcc ggcgatactg ttttcatttc caaatatcat actgaacagc
     4921 gtgacggcgg caaagcagaa ggcgacaacc agatagacct tgaagttgta gataagaatg
     4981 tggcgcatac gttccttttt gtcccgggtg atccggatca gggctttgga accagcggaa
     5041 tttaattgca gttcctgata aaatgtcatt tgtaaaaacc tgccactcaa aatgtgttca
     5101 actatactca aacacatcga gaaattaaag tcctcacgga tactttttac tgcttcattg
     5161 tgtatgcgtt ggcagttgct ggcaacttgc tactaacagg ttcttgtaca ctccacagtc
     5221 gtcaattccc gaaatagcct tcggtacata cttacacttg cctgatcatg caattttgta
     5281 agttactgca tcttttaaat taagactcgc attaagatct ctatcctcta cataactaca
     5341 actgcatttg tagatcctgt ctgaaagttt taagtccttt tttatagaac ctcaacaatg
     5401 acatatttta gaagatggat aaaacctgtc tgcaattctt aattcaatgc cattatcatc
     5461 acacttcact ttcaactttg tcctaaactc atttctgtga tgcaactgct tttgaaagat
     5521 gccggttctt catcattcct gatacattca gatcctcaat cgttatataa gacggcttgg
     5581 ttttcactat ctcagcgatg gccttattga tatgatcagt tcggatattg tctatccgat
     5641 gatgaagttt ctgtacctta agcttttgct tttgtatatt tgctttttga gtggactctc
     5701 ctttctttaa attttcatac ttacgggaga gacatctctg ctcccggatc agttgttttt
     5761 caagtttttt cagtcttgct catttattaa tgttcttata tttcttacca tttgagagaa
     5821 tggcaaaatc ttttaatcca aggtctatgc caatcccttc gctgtagata tcggttgttt
     5881 tgttatccgg tatttctatc agaactgaaa cataaaacct gtccgaagta catttttaca
     5941 tccgacttac cctttttttg aatttaggaa agccgctttg atgtttgaaa aatcttgtaa
     6001 atgcggtctg tccatgattt acagactggg ttactgcttt tgaatatgcc tccttgatcc
     6061 atgcatattc cgggtgatcc ggcagatatt cattattaag ccagactcta aattg
//
LOCUS       DBMDUH010000157         6059 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_5259_length_6059_cov_3.656260, whole genome shotgun sequence.
ACCESSION   DBMDUH010000157 DBMDUH010000000
VERSION     DBMDUH010000157.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 6059)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6059)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6059
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5259_length_6059_cov_3.656260"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(156..959)
                     /locus_tag="ACZPCG_11245"
     CDS             complement(156..959)
                     /locus_tag="ACZPCG_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HIR1285665.1"
                     /translation="MDRIEEICDKIGQCDDNIIEQLVARMRCIQDIIEYKKEKGIPIL
                     QPEQEKKQEDNLKRKLGDNPFEEEILDIFKYIVKNSRRIQAKALFNYNIFLIGFMGAG
                     KSTVARELKKQLEMNYVEMDQLIVEQRGMAITDIFEEYGETYFRNLESNVLIELQKRK
                     QTIVSCGGGIVVREDNKDHMKKNGRVVFLTATPNTVYERVKNSTERPILNNNMNVEFI
                     SQLLEKRRALYEEAADITVATDGKSVSEICDEIVSELIAYDLRLNKKES"
     gene            complement(1049..1849)
                     /locus_tag="ACZPCG_11250"
     CDS             complement(1049..1849)
                     /locus_tag="ACZPCG_11250"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR1285666.1"
                     /translation="MGPVTVSFCRYGIALICLYGILKLKGCHKKVQKEHWPYLMILGG
                     LGYFAAIILQLAGTARLSGSLSSLMNSLNPVAISVMAAIFLKERITWKNAIGIVISLA
                     GVYVILGTGELQADLMGILFSAGSVILWSAASVSIRRISEYYDPVQTALYGMAIALIL
                     NVPAAVFENMFIFRSHPTEKALLACLFVAVVGTAVAHTFWNKSLQQLSASTCSMFYPL
                     QPLVSAVLGVVLLHEIITWQFVAGAVLICSGIVINFLPFMPNKEKIAR"
     gene            complement(1994..2860)
                     /gene="aroE"
                     /locus_tag="ACZPCG_11255"
     CDS             complement(1994..2860)
                     /gene="aroE"
                     /locus_tag="ACZPCG_11255"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HIR1285667.1"
                     /translation="MEISGHTQMLCLIGSPVGHSGSPAMYNYSFQKLGLDYVYLAFDI
                     KEEDTSKAINALRLLNVKGFNITMPGKTAALSCCDELSKAAQLIGAVNTVVNENGKLI
                     GHNTDGLGWVRNCREHGFEIRGKKMTIAGSGGAATAIEITSALEGMSEISIFARKDTF
                     FANAEATVEKIRRHVSGCRVNLYDLEDRDLFYHEIEDSHIFTNATRVGMKPMDQESLI
                     ENPEVFRSDLTVSDVVYNPRETRLLSDAAVAGCKVIPGIGMLLWQGAEAFKLYTGKEM
                     PVKEIMEKYFAV"
     gene            3528..3923
                     /locus_tag="ACZPCG_11260"
     CDS             3528..3923
                     /locus_tag="ACZPCG_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285668.1"
                     /translation="MIFLPFSILSSQNPLVFLLFPALCILFLFLQFLFVRLLFRIAFY
                     KDYASFNQMEKESLSSYYQNLNGTLCAMQNIRHDIKNIFFTMGNFVNESENQEMKDFF
                     WGKIFPCSQDTIRQSELLSSICQLPSEPL"
     gene            3954..4346
                     /locus_tag="ACZPCG_11265"
     CDS             3954..4346
                     /locus_tag="ACZPCG_11265"
                     /inference="COORDINATES: protein motif:HMM:NF025854.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HIR1285669.1"
                     /translation="MQQKCSISLHIQVLPETWQTGMDLIDLTRILGILIDNAIEEVLL
                     VPDGIIEVRITGNACGCSYTIRNSVTPRTIQQGIHAGISTKGEGRGKGLSTVRELLSP
                     YHNATLNSCLQGDIFIQSLTLSWQDPLS"
     gene            4477..>6059
                     /locus_tag="ACZPCG_11270"
     CDS             4477..>6059
                     /locus_tag="ACZPCG_11270"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012869.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1285670.1"
                     /translation="MKRLIWKPFFHLLLQARLPWFYILVYTLLSMAASQLYLLFPSYT
                     RQIMNGSLNPALIYTVIAVVSGQACITSVSQFILAAASAKVSLRLRELIWRQILRLPV
                     PFFDAQPSKDLISRVTQDTAKLSDLAVSFPGNVLSSLYTFFGSFVLLFSYHWKLAALE
                     AVMIPLLYALGVWQGKIQFSWNERIQGKNAGLTGHLSEILSNLPLVKEFCAEEWEKKR
                     GNHWIHQLFQVNISNSLITNLLLQLKNLASLLHTTAMIGLGLWMISHNEITLDIWVAF
                     YLYSQNLLNSFSRIMSIWGTVKSSQGAVRRITELMAEQTEPDDPCTGSDGMKGDILFE
                     NVSFSYENQPVLKNLTFTAPEGKTTALVGPSGAGKTTFFQLLERFYSSDQGSIRMGKT
                     DIFKLPLVQWRRMIGYGPQSPYLFSGTIRENILYGIHRPVTEQELLQAIEDSQCSDFI
                     RAMPHGLMTRIGEGGSGLSGGQRQRISLARLFLQNPKILLLDEVTANLDPESEYRVEQ
                     ALARLRKGRTTFVIAHRTSA"
BASE COUNT         1563 a         1575 c         1097 g         1824 t
ORIGIN      
        1 ttttgcggct tttttccgtt tttttttttt tttacttcac gatttaccct tttacatttt
       61 ctgtcgaaat atgggattaa cccccacttt taatagtttt gtaataaaaa agtttctgtc
      121 cgataattct attgcgaaac agaaactctc ttttatcatg attctttttt atttaatctc
      181 aggtcataag caatcagctc tgaaactatt tcatcacata tttcggaaac agattttcca
      241 tctgttgcta ctgtgatatc cgctgcctct tcatacaaag ctctgcgttt ttctaaaagc
      301 tgtgaaataa actctacatt catattatta ttcaggatcg gacgttctgt actgtttttc
      361 acccgctcat aaacagtatt gggcgttgca gttaaaaaca caacccgccc atttttcttc
      421 atatgatcct tattatcttc ccggactaca atacctcctc cacaggaaac gatcgtctgt
      481 ttacgctttt ggagttcaat cagcacattg ctttccagat tacgaaaata tgtttcacca
      541 tattcctcaa aaatatctgt gatggccatt cctctctgct ccacgatcag ctgatccatt
      601 tcaacgtaat tcatttccag ctgttttttt aattctctag ccacagtgct tttccctgcg
      661 cccataaagc caataaggaa aatattataa ttaaacagag ctttagcctg aatccttcgg
      721 ctgttcttca caatgtattt gaagatatcc agtatctcct cctcaaaggg attatctccc
      781 agcttacgct tcaggttatc ctcctgcttt ttttcctgct ccggctgcag gatcggaatt
      841 cccttttcct tcttatattc aataatatcc tgaatacatc tcattctggc aaccagctgc
      901 tcaatgatat tgtcatcaca ctgtccaatt ttatcacata tttcttcaat tctgtccatt
      961 gcactctcct ctgtcttctt cgtcacacga attatttttg aatgcaatta tatcagcaga
     1021 acctaaaaag tgcaatgatt ttatcgcttt aacgcgctat tttctcttta tttggcataa
     1081 aaggcaggaa attaatcaca attccagaac agatcagtac agcgcctgct acaaactgcc
     1141 atgtaataat ctcatgaaga agcactaccc caagtactgc tgataccaac ggctgtaaag
     1201 ggtaaaacat agaacaggta cttgcactca gctgctgaag gctcttattc caaaaggtgt
     1261 gtgctaccgc agttccaact accgcaacaa acaggcaggc cagcaatgct ttttctgtag
     1321 gatggcttct gaaaatgaac atattctcaa atactgctgc cggtacgttt agaatcaggg
     1381 caatggccat accgtaaaga gcggtctgaa ccggatcata atattcggag attctgcgaa
     1441 tggatacaga agcagctgac cagagaatta cagagcctgc ggaaaacagg attcccatga
     1501 ggtcagcctg cagctctccc gttcctaaaa taacatatac gcctgcaaga gatataacaa
     1561 tgccgatcgc atttttccat gtgatccttt cctttaaaaa tatcgccgcc ataacagaaa
     1621 tcgcaacggg attcaaagaa ttcatcaggg aagaaagcga tcctgacaat ctggcggttc
     1681 cggcaagctg cagtataatt gctgcaaaat atcccaagcc tcccagaatc atcaaatacg
     1741 gccagtgctc tttctgaact tttttatgac atccttttaa ctttaaaata ccatacagac
     1801 agatcagagc tattccatac cggcaaaaag aaactgttac cggccccata acctccaaag
     1861 catacttgcg ctcacaacat aaatacttcc ccagcaaaaa aaggtaatca acaaatacag
     1921 ataccctaca ttacgcttca tgttccctgt tccttctttc tgttatcagc agctttattt
     1981 tatgggcggt tcattatacc gcaaaatatt tttccattat ttccttaacc ggcatttcct
     2041 tacctgtata aagtttaaat gcctctgccc cctgccataa aagcatccca attcccggaa
     2101 tcactttaca ccctgccacc gcagcatctg acaggagtct ggtttctctc ggattataaa
     2161 ctacatctga cacagtcaga tcactgcgaa aaacttcagg attttcaatc aggctctcct
     2221 gatccatagg cttcattccc acccgggtgg cattggtgaa aatatgactg tcttctattt
     2281 catgataaaa taaatccctg tcttccaaat catacagatt cacccggcat cctgaaacat
     2341 gcctgcggat cttttctacc gtagcctctg catttgcaaa aaaagtgtcc tttcgtgcaa
     2401 atatagaaat ctcactcatc ccttctaaag cagatgtaat ctcaatggca gtagccgctc
     2461 caccggaacc ggcaatcgtc attttctttc cccttatttc aaagccatgc tcccggcagt
     2521 ttctgaccca gcccaaaccg tccgtgttat ggccaattag tttgccattt tcatttacta
     2581 ccgtatttac cgctccgata agctgtgctg cttttgaaag ctcatcgcag caggaaagcg
     2641 ctgccgtctt tcccggcata gtaatattaa agccctttac gtttaataac cgaagcgcat
     2701 taatagcctt tgatgtatcc tcttctttta tatcaaaagc caaatacaca taatccagcc
     2761 ccaatttttg aaaactataa ttatacatag ccggcgatcc cgaatgcccg acagggcttc
     2821 ctatcaggca cagcatttgc gtatgtccgc taatttccat aactcatttc ctccctgtct
     2881 taattttggt ctattttacc ccaatctcat agaatatgca agtgcattat ttctttatca
     2941 aatttaaaaa acagggttat tcagaacaag gccttttcct tcttatggct ttatcgaaaa
     3001 atccctggag cgtccaaaga tgattcatga atttacccgc tgtttgacgc ttgccgctcc
     3061 ccaaaaaatg atgaaaaccc cacgtctttc attgaaatcc cctttctttt ttcagccttc
     3121 tttttctctc aaagactgtt taactctgcc tccgtactgc tgggacacct ctggctgttc
     3181 tggctgattt tctgcgtata ttacactgcc gtaaaattaa gcctccacag cctgcgcgga
     3241 aaccagtttt cctgctatct ctcctttgta atcctgtccg tttctgccta cggattcttt
     3301 ctggtaaact attttgccgt cccttatctt caaaaagcag tcggaagcta tgcgctgggt
     3361 ctcatcggca ctaccgggct gtattctctc ctgcttcttg gagcgatcct tttttccgtc
     3421 cgcgtttttt taaaccgatc gaacagttaa tacagcttgg aaagaaatat cccgctatcg
     3481 aaccctacta ttttctcttt tccgccttaa tccttgtgtt ctgtacgatg atctttttac
     3541 ctttttccat cctgtcctcc cagaacccgc tggtttttct tttatttccg gctctgtgca
     3601 tcttatttct gtttctgcag tttttattcg tccgcctttt gttccggatc gccttttata
     3661 aagattatgc ttcctttaat cagatggaaa aggaaagtct ttcctcctac tatcagaacc
     3721 tgaacggaac actctgtgcc atgcaaaata tccgccatga cataaaaaat attttcttca
     3781 ctatgggaaa ttttgtaaat gaaagcgaaa accaggagat gaaagatttt ttctggggaa
     3841 aaatctttcc ctgctcccag gataccatcc gccaaagcga actgctgtca tccatctgcc
     3901 agctgccctc cgaacctctg taggccttct tcgttttaaa aacttctcag gctttgcagc
     3961 aaaaatgttc catttccctg cacattcagg ttctccctga aacctggcag accggtatgg
     4021 atctgatcga tctaacccgt attctgggaa tcctgattga taatgcaatt gaagaggttc
     4081 ttctggttcc cgatggcatc atcgaagtcc gcattacagg aaatgcctgc ggctgctcct
     4141 atacaatccg aaattccgtt accccgcgta caatccagca gggcatccat gccggaatca
     4201 gcaccaaagg agagggacgc ggaaaagggc tttccactgt ccgtgaactt ttaagccctt
     4261 atcacaatgc caccctgaat tcctgccttc agggggatat ctttatccag agccttaccc
     4321 tgtcatggca ggatcctctg tcctgaaccc tcagaaaatg tcctgaactc aatctataat
     4381 cctgttttta cgacaccgca cacgccattt aagccaaatc ccacacggat ttggcttttt
     4441 atgttatgat ccgtcagacg aaaggagacg cgatctatga aacgacttat ctggaaacca
     4501 tttttccatc tgctgcttca ggccagactt ccctggtttt atatcctggt ctatacgctt
     4561 ctgtctatgg cagcaagcca gctgtatctg ctttttccct cctatacccg gcagatcatg
     4621 aacggcagcc tgaatccggc gcttatttat acagtaattg ccgttgtttc aggacaggca
     4681 tgtattacct ctgtcagcca gtttatttta gcagctgcaa gtgcaaaggt ttctctgcgc
     4741 ctgcgcgagc ttatctggag acaaatcctc cgccttcctg ttcctttttt tgacgcacag
     4801 ccctcaaagg atctgatcag ccgcgtaacc caggacaccg caaagctcag tgacctggct
     4861 gtttcttttc ccggaaatgt gctgtcctcc ctctacacgt ttttcggttc ctttgtgctc
     4921 ctcttttcct atcactggaa gcttgctgcg ctggaagccg ttatgatccc ccttctgtat
     4981 gctctcggcg tatggcaggg aaaaatccag ttttcatgga atgaaaggat tcagggaaaa
     5041 aacgccgggc ttaccggtca tctgtccgaa atcctgtcca atctgcctct ggtaaaagaa
     5101 ttttgtgcag aagaatggga aaagaaacgg ggaaaccact ggattcatca gttgtttcag
     5161 gtaaatattt ccaattctct tatcactaat cttctgctcc agttaaaaaa ccttgcttca
     5221 ctgctccata caactgccat gatcgggctg ggactgtgga tgatctccca taatgaaatc
     5281 acccttgata tctgggttgc attttatctc tactcccaga accttttaaa ctcattttcc
     5341 agaatcatga gcatctgggg aaccgttaaa tccagtcagg gagcagtccg ccggatcaca
     5401 gagcttatgg cagaacagac ggaacctgat gatccctgca ccggttcaga cgggatgaaa
     5461 ggagatatcc tgtttgaaaa tgtatccttt tcctacgaaa atcagcctgt actgaaaaat
     5521 cttaccttta cagccccgga ggggaaaaca acggccctcg tcggtccaag cggggccggt
     5581 aaaactactt tttttcagct tttggaacgc ttctattctt cggatcaggg aagtatccgc
     5641 atgggtaaga cagatatttt taaactccct ctggtacagt ggcggcgtat gatcggctac
     5701 gggccccaga gcccctatct tttttccgga acgatccgcg aaaatatcct ttatgggatt
     5761 caccggcctg taactgaaca agagctttta caggccatag aggattccca gtgcagtgat
     5821 tttatacgtg ccatgcccca cggccttatg acccggatcg gagaaggcgg cagcgggctc
     5881 tccggagggc agcgccagcg tatctctctt gcgcggctgt ttctccaaaa ccccaaaatc
     5941 cttcttttgg atgaggtgac tgcgaacctt gatccggaat cagaatacag ggtggaacag
     6001 gcccttgccc gtctcagaaa aggacggacc acattcgtca tcgcccacag aacgtccgc
//
LOCUS       DBMDUH010000158         6008 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_5297_length_6008_cov_7.196208, whole genome shotgun sequence.
ACCESSION   DBMDUH010000158 DBMDUH010000000
VERSION     DBMDUH010000158.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 6008)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6008)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6008
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5297_length_6008_cov_7.196208"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="ACZPCG_11275"
     CDS             complement(<1..262)
                     /locus_tag="ACZPCG_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285671.1"
                     /translation="MAFKVVLVDDDLLVLQDLETLADWGKYGFTIAQTFTNGVAALRG
                     IREIRPDFIVKLIRFHESLLLQCRRVRMISYLERKYQDKAYRD"
     gene            complement(462..2216)
                     /gene="thrS"
                     /locus_tag="ACZPCG_11280"
     CDS             complement(462..2216)
                     /gene="thrS"
                     /locus_tag="ACZPCG_11280"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HIR1285672.1"
                     /translation="MKIILPDGNVQEAEDELRAIRHTASHVLAQAVKRLYPETKLAIG
                     PAIDDGFYYDFDREGGFTPEDLEKLEAEMKKIIKENIPLKPFVLPRDEAIKLMQEKGE
                     PYKVELIEDLPEEETISFYQQGDFTDLCAGPHIMYTKGVKAFKLTSIAGAYWRGSEKN
                     KMLTRIYGTAFANKEDLENYLTMLEEAKKRDHRKLGKELGLFMFTEEGPGFPFFLPKG
                     MTLKNTLIDYWREIHLRENYQEVSTPVILSRKLWETSGHWDHYKDNMYTTVIDEEDYA
                     IKPMNCPGGMLVYKSQPRSYRDLPLRVGELGLVHRHEKSGQLHGLMRVRCFTQDDAHI
                     FMTQEQITEEIKNVTRLINEVYTLFGFEYFVELSTRPEDSMGSDEDWELATNGLRNAL
                     EEMGLDYIVNEGDGAFYGPKIDFHLRDSIGRTWQCGTIQLDFQLPQRFEAEYVAEDGT
                     KKRPIMIHRVCFGSIERFIGILIEHFAGKFPVWLAPVQVKVIPVSEKSMEYATGVYEK
                     LRAAGIRTELDHKDEKVGYKIRQAQLEKVPFMLVLGEKEAAEGAITVRSRDKGDLGSA
                     QLDEFIADVKKMVESRER"
     gene            2694..3116
                     /locus_tag="ACZPCG_11285"
     CDS             2694..3116
                     /locus_tag="ACZPCG_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HIR1285673.1"
                     /translation="MKLVIIDGQGGKMGRTVIEQLKKNLPDLEIYAIGTNSIATSAML
                     KAGADYGATGENPVIVGARTADIIIGPIGIVIADALMGEITPSMATAIGQSPAYKILI
                     PVNRCNHYIPGCGDHNLGEYIKMVCEETEKEVKKRENR"
     gene            3103..3336
                     /locus_tag="ACZPCG_11290"
     CDS             3103..3336
                     /locus_tag="ACZPCG_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007708358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285674.1"
                     /translation="MRTVEFKMERPGLVSPGDKVKIIEREGTSSYSYIIDPAVAMSGC
                     FAARDRIKSEYGIVKDVRENARGFYVVVDFDED"
     gene            complement(3333..5180)
                     /locus_tag="ACZPCG_11295"
     CDS             complement(3333..5180)
                     /locus_tag="ACZPCG_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5050 domain-containing protein"
                     /protein_id="HIR1285675.1"
                     /translation="MGRRRKSDSSGKTILLVLLAAFFLIAAIYVVVQVIGTIHKDYSL
                     IEQKEDSSTETAQTIEIEHEEETQGWNETERGWTYKLDDETWATDQWLEIEGFLYHFN
                     EDGIMNQGKWQKEGQIYTCHDVKGYLKNIETDLDYVPDDKGENLDSLARTNAFWCFLD
                     DEDTGIFKTILYRKTVENKVKPLGDENAPERTTKYSLRADGDYVYYLPLVKENEKGKL
                     TESEKALCGRLVRMIPGQAVKEIIAEDVDGYLIVDGTIYYAQGGKIHSATSGTETAVG
                     EESYKVQIRDGSCYLTDSLGNIVTLENGTVMEMGDRSYRLEENGAIRYVKRRDVSVNG
                     VTFRLKGEGTGMMVSMESDGASRAAITGEYGVQSFCIVDNFIFYCAYVDKGANGEWYS
                     RIFRADLNGEGKTPVSGMFPGTITNLYYFENEGQMYGEYYPEIWKNAYGTIVNVSQSG
                     TIYRINDANVRTGIKVSGNDMLELVMAQDGKLICLWHDCSWSPKAGITKVLWSRPVEF
                     SASEKISVETVSKLPEQTAAPAEKETTKVAVETVPVNPQQVSGSNQISPAETTAAPVK
                     NPNQPADTPGTSVQTPKQETILGTEAPGKSIQTNPSETKSDEVKIVPLG"
     gene            complement(5266..5338)
                     /locus_tag="ACZPCG_11300"
     tRNA            complement(5266..5338)
                     /locus_tag="ACZPCG_11300"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5303..5305),aa:Lys,seq:ttt)
     gene            complement(5354..5426)
                     /locus_tag="ACZPCG_11305"
     tRNA            complement(5354..5426)
                     /locus_tag="ACZPCG_11305"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5391..5393),aa:Phe,seq:gaa)
     gene            complement(5453..5526)
                     /locus_tag="ACZPCG_11310"
     tRNA            complement(5453..5526)
                     /locus_tag="ACZPCG_11310"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5490..5492),aa:Met,seq:cat)
     gene            complement(5579..5660)
                     /locus_tag="ACZPCG_11315"
     tRNA            complement(5579..5660)
                     /locus_tag="ACZPCG_11315"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5624..5626),aa:Tyr,seq:gta)
     gene            complement(5703..5775)
                     /locus_tag="ACZPCG_11320"
     tRNA            complement(5703..5775)
                     /locus_tag="ACZPCG_11320"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5740..5742),aa:Val,seq:tac)
     gene            complement(5814..5887)
                     /locus_tag="ACZPCG_11325"
     tRNA            complement(5814..5887)
                     /locus_tag="ACZPCG_11325"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5851..5853),aa:Asp,seq:gtc)
BASE COUNT         1534 a         1651 c         1116 g         1707 t
ORIGIN      
        1 ggtccctgta tgccttatcc tgatattttc gttccagata ggaaatcatc cttacccgac
       61 ggcactgaag gagcaggctt tcatgaaaac gtatcagctt tacaataaaa tccggtctta
      121 tctctctgat tccccgcaat gctgccaccc cgttcgtaaa ggtctgagca atagtaaatc
      181 cgtattttcc ccagtctgcc agtgtttcca gatcctgaag caccagcagg tcatcatcca
      241 ccaaaaccac cttaaatgcc acgtttcctc ctccatacag ccaaatcttt gccgcccagt
      301 tactgagact gataactctt atatacacat attttaccat gtggggaatt ttgtgtcatc
      361 tatgagattt tatcaggtat tcttcccctc ctgtaaaaaa caggcgcacc cggtaaacga
      421 atgcgcctgt tttatcattt tataatcttc agaaaataca attatctctc tctgctttct
      481 accattttct tcacatctgc gataaactca tccagctgtg cgcttcccag atcacccttg
      541 tcacggcttc tcactgtaat tgcgccctca gcggcctcct tctcgcccag taccagcatg
      601 aagggtacct tttcaagctg tgcctgacgg atcttgtagc ccaccttctc atccttgtga
      661 tccagctctg tacggatacc ggctgctctc agcttctcat atacacctgt tgcatactcc
      721 atagattttt cagatacagg aattaccttt acctgaaccg gagccagcca tacagggaac
      781 ttacctgcaa aatgctcgat cagaataccg atgaagcgtt caatggaacc aaagcataca
      841 cggtgaatca taatcggacg cttctttgtg ccgtcctcag ccacatattc agcctcaaaa
      901 cgctggggca gctggaaatc caactgaatg gttccgcact gccatgtacg tccgatggaa
      961 tctctcagat ggaagtcaat cttaggacca tagaatgcac cgtcgccttc attaacaata
     1021 tagtccagcc ccatttcctc cagagcgttt ctcaggccgt tggtagccag ctcccaatcc
     1081 tcgtcagatc ccatggaatc ctcaggtctg gtagacagct ctacgaaata ttcaaagcca
     1141 aaaagggtat aaacctcatt gatcagtctt gtaacgttct taatctcctc agtaatctgc
     1201 tcctgagtca taaagatatg ggcgtcgtcc tgggtaaagc agcgcacgcg catcaggccg
     1261 tgaagctgac cgctcttctc gtgacggtga accagaccca gttcgcctac tctcagagga
     1321 agatctctgt aggaacgcgg ctgagacttg tacaccagca taccgccggg gcagttcatt
     1381 ggcttaattg cgtagtcttc ctcatcgatc acagtggtat acatattgtc cttatagtga
     1441 tcccagtgac cggaggtctc ccacagcttg cggcttaaga tcacaggagt ggaaacctcc
     1501 tgataattct ctcttaaatg gatctctctc cagtagtcga tcagggtatt ctttaaggtc
     1561 atgccctttg gaaggaagaa tgggaaacca gggccttcct ctgtaaacat aaacagaccc
     1621 agctccttgc ccagctttct gtggtcgcgc ttctttgcct cctccagcat ggtcaggtag
     1681 ttttccagat cctccttatt tgcaaatgca gttccataaa ttctggtcag catcttattc
     1741 ttctcgctgc ctctccagta agcgcctgca atgcttgtga gcttaaatgc ctttacgccc
     1801 tttgtataca tgatatgggg gcctgcgcac agatctgtaa aatctccctg ctgataaaag
     1861 ctgatggtct cctcttcagg cagatcctca atcagttcta ccttgtaagg ctcgcccttt
     1921 tcctgcatta acttgattgc ctcatctctt ggaagcacaa atggctttaa tggaatattt
     1981 tccttgatga tcttcttcat ctcagcctct aacttctcca gatcctctgg ggtaaatccg
     2041 ccctctctgt caaagtcata gtaaaagcca tcatcaatag ccggtccgat ggctaatttt
     2101 gtctccggat acagtctctt tacagcctgt gccagcacat gggaagcggt atgacggatc
     2161 gcccttaact catcctctgc ctcctgtaca tttccatctg gtaaaatgat tttcatggta
     2221 aaactccttt cttaatcgtc aggccccttt tcagagccct attatgatct ttgaaaatgg
     2281 tcccgttcgt tctggcctgc ggccaaataa aaaccccctg gacacatttc catgtccaag
     2341 ggtgcatata cacggttcca ccttgatttt cactcattga tcccttaacg cggggtacgg
     2401 tgccatttcc tctgttttca ctcaaacgcc aaaggcgcag ccacgaaaac agcatccttg
     2461 ccacagctca ggactggtct tcctctgcat tttccacaca gcctttccac cacccggcta
     2521 ctctctgtct gttccctgca aagtacttgt tccttcaaag cttcctgcat ttctgctcat
     2581 ttctgattat aacatctaaa ggttatttgt caagactgct tttccttgag tttcccatcc
     2641 atctctggta atattctata gtaaatcaca gcagaaagaa ggtattttca caaatgaaac
     2701 ttgtcatcat agacggtcag ggcggtaaaa tgggccgaac cgtcattgaa cagctgaaaa
     2761 aaaatctccc agatcttgaa atctacgcca ttggaaccaa cagtattgct acctctgcca
     2821 tgttaaaggc aggcgcagac tacggcgcta ctggtgaaaa tcccgtaatc gtaggagccc
     2881 gcacagccga tattatcatc ggccctatag gaattgtaat tgcagatgcc ctgatgggcg
     2941 aaataactcc gtctatggct actgccatcg gacaaagccc cgcttataaa attctcatac
     3001 cagtcaaccg ctgtaatcac tatatccccg gctgtggaga tcacaacctt ggagaataca
     3061 tcaaaatggt atgcgaagaa acagaaaagg aggtcaaaaa acgtgagaac cgttgaattt
     3121 aaaatggaac gtcccggact ggtaagccct ggcgacaaag taaaaatcat tgagcgcgaa
     3181 ggtaccagca gttacagcta tattattgat cctgctgtcg ccatgtccgg ctgttttgca
     3241 gcccgggacc gcattaaatc cgaatacggt attgtaaagg atgtccgcga aaatgcccgg
     3301 ggcttctatg tggtagtaga ttttgatgaa gattagccta aaggcacaat cttcacctca
     3361 tctgactttg tttctgaagg attcgtctgt atactctttc ccggagcctc tgttcctaaa
     3421 attgtttcct gcttgggagt ctgaaccgaa gtgccgggag tatctgccgg ctggttcgga
     3481 ttcttcacag gtgcagctgt tgtttctgcg ggagatattt gatttgaacc ggacacctgc
     3541 tgcggattca ccggaaccgt ctcaacagct acctttgttg tctctttctc agcaggagct
     3601 gcagtctgct caggcagttt tgaaaccgtt tccaccgaaa tcttttcaga tgccgaaaat
     3661 tccaccggac ggctccaaag caccttggta atccctgctt tcggactcca gctgcagtca
     3721 tgccacagac aaatcagctt gccatcctga gccatcacaa gctccagcat atcattaccg
     3781 cttaccttta ttcccgtacg cacattcgca tcgttgatcc tgtaaatggt tccactctga
     3841 ctcacattta caatcgtacc ataggcattc ttccatatct ctggataata ttctccgtac
     3901 atctgccctt cattttcaaa ataataaaga ttcgttatgg ttcccggaaa cattccgcta
     3961 accggcgttt ttccttctcc attaagatct gctctgaaaa tccggctgta ccattctcca
     4021 tttgctcctt tatccacata agcacagtaa aaaataaaat tatccacaat acagaagctt
     4081 tgtacaccat attctcccgt aatggctgct ctggaagctc catccgattc catcgacacc
     4141 atcataccgg ttccctctcc ctttaaacgg aaagtaacac cattaacaga tacatctctg
     4201 cgctttacat agcgaattgc cccgttctcc tccagacggt agcttctgtc acccatttcc
     4261 atcacagttc cattttcaag tgtcacaata tttcccagac tgtctgtcaa ataacagctt
     4321 ccgtcccgga tctgaacctt atagctttct tcaccaactg cggtttctgt tcctgatgtg
     4381 gccgaatgga tctttccccc ctgggcatag taaatcgttc cgtctacaat cagataccca
     4441 tcgacatcct ctgcaataat ctcctttaca gcctgtcccg gtatcatccg taccagccgc
     4501 ccgcaaagcg ccttttcaga ttctgtcagc ttgccttttt cattttcctt aaccaaagga
     4561 agataataca cataatctcc gtccgctctc agagaatatt ttgtagttct ctcaggagca
     4621 ttctcatccc ccaggggctt aaccttattt tcaaccgttt tacgatacag aatcgtttta
     4681 aaaatccccg tgtcctcatc atccagaaaa caccagaaag catttgttct tgcaagactg
     4741 tccagatttt ctcccttatc atcaggaaca taatccaaat cagtttctat gttctttaga
     4801 tagcccttca catcatgaca ggtataaatc tgtccttcct tctgccactt tccctgattc
     4861 atgattccat cttcattaaa atgatataaa aaaccttcaa tctccagcca ctgatctgtt
     4921 gcccaggttt catcatccag tttatatgtc catcccctct cggtctcatt ccatccctgt
     4981 gtttcttcct catgctcaat ttcaatggtc tgtgctgttt ctgtactgct gtcctctttc
     5041 tgctcaatca aactgtaatc cttatgaatt gtaccaatta cctgaacaac cacataaatt
     5101 gccgcaatca aaaagaatgc tgccagcagc actaaaagaa tcgtcttgcc actggaatct
     5161 gactttcttc ttctgcccat atcttaatcc tttctctgaa attcactttt cagcataaac
     5221 aaaaaaagcc gtttttcttt gcaaatacaa tgaaaaacga cctactgagc gtgccgggat
     5281 tcgaacccgg gacaacttga ttaaaagtca agtgctctac cgactgagct acacgccctt
     5341 attaaattta aaatgcccaa agtcggaatc gaaccaacga cacgaggatt ttcagtcctc
     5401 tgctctacca actgagctat ctgggctttt tcttatgcct gagcataaaa aattgcgggg
     5461 gcaggatttg aacctacgac cttcgggtta tgagcccgac gagcttccag actgctccac
     5521 cccgcgctaa catatttagt ataaatgcct gatgacaatt tgtcaacctg cattttaatg
     5581 ggcgaaggtg gattcgaacc accgaaggca gtgccagcag atttacagtc tgtccccttt
     5641 ggccactcgg gaattcgccc atattgatat tatatacatt ttttgataaa taatacattc
     5701 aatggggcct acagggctcg aacctgtgac cctctgcttg taaggcagat gctctcccag
     5761 ctgagctaag accccaactg taaaattatc agcaaatgta ttaagaagaa aaacgacccg
     5821 gaacgggttc gaaccgtcga cctccgccgt gacagggcgg cgctctaacc agctgagcca
     5881 ccgggccatt tttaaaggca tatacacacc ttcaaaacca catactgaac aaccaagcca
     5941 caaccgtttt ccctaaacct tctggtcaag ccctcgaccg attagtatca gtcagctcca
     6001 tgcattgc
//
LOCUS       DBMDUH010000159         6005 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_5302_length_6005_cov_4.772019, whole genome shotgun sequence.
ACCESSION   DBMDUH010000159 DBMDUH010000000
VERSION     DBMDUH010000159.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 6005)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6005)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6005
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5302_length_6005_cov_4.772019"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="ACZPCG_11330"
     CDS             <1..672
                     /locus_tag="ACZPCG_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1285676.1"
                     /translation="RDLLSGIIVMTALYSINLRITGKANLPIFSKETIFSNEFLSANV
                     PEAFKPYTTTLILLVLAVLCKVLLDAYLKTRSGYLLRAVGDNETLVTSLAKDKGLVKI
                     IGLAIANGFAALAGCVYCQQKGFFEISAGTGTMVIGLANVIIGTQLFKRFGFVKSTTA
                     VIIGSILYKACVSAALLVGDLKIAGLNLSVPVTASDLKLITSVLFLIILVLSPAGGKK
                     VKSHA"
     gene            665..1438
                     /locus_tag="ACZPCG_11335"
     CDS             665..1438
                     /locus_tag="ACZPCG_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1285677.1"
                     /translation="MLELKHIHKYYNTGTVNEMCLFEDFNLTIRDHEFVSVVGSNGSG
                     KTSLLNIICGSIPLDEGTIRIGNEDITKMAEYKRQRRIGRVYQNPAMGTCPSMTIAEN
                     MALADNKGKFFNLRPGKNKQRIDFYRESLRTLGLGLEDKMDVKVGVLSGGQRQAMALL
                     MSTMTPIEFLILDEHTAALDPKTAETIMELTDRIVREKNLTTIMVTHNLRYAVDYGNR
                     LLMMHQGKAIMDKAGTEKEETRIDDILKKFNEISIECGN"
     gene            complement(1497..2210)
                     /locus_tag="ACZPCG_11340"
     CDS             complement(1497..2210)
                     /locus_tag="ACZPCG_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285678.1"
                     /translation="MITLRRYYFCVAAICAAAVIGISGCAMNTKAESAQTQADASQPE
                     SAEAATVVEAVTGPIESMEETPNEELQPVRIYGSASHMEDGRLSIDNRSGVSAEGEII
                     LNISQESTLILDAVSGTPLTLEDIQDGETIYAYIGPAMTMSLPPMTNAAIIFANIPAD
                     FKVPDYVEADSVITDAGSSQTVLTASDGTEYILAEDCDIFPYLTRNIVTLDDLTQGKK
                     VVVWSGDDNTATKIMVFAE"
     gene            complement(2328..3362)
                     /locus_tag="ACZPCG_11345"
     CDS             complement(2328..3362)
                     /locus_tag="ACZPCG_11345"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HIR1285679.1"
                     /translation="MAEYTSRSFDLLTLGQLLLRLSPPDNDRLSRGDTFVKQVGGAEL
                     NVAVGASLLGLRTGVISKLPSHDIGSFMKGKIRSFGVSDDFFMYDDSPSARVGIYYYE
                     NGAYPRKPKVIYDRMNSSFFSLNIDEIPKEVYTASKCFHTTGITLALSPQIRETTIEM
                     IKRFKEAGTLVSFDVNFRGNLWSGDEARECILKILPYVDIFFCSEDTARLTFLKTGTA
                     KEMMKSFTEEYPISIVASTQRIVHSPKRHTFGSVIYDAARDTYYEEEPYRDIEVVDRI
                     GSGDAYISGALYGLLSSGGDCSKAIAFGNATAAVKNTIPGDLPSSNLEEIQTIIKAHN
                     QSGPQSEMAR"
     gene            complement(3486..4517)
                     /locus_tag="ACZPCG_11350"
     CDS             complement(3486..4517)
                     /locus_tag="ACZPCG_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013498824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HIR1285680.1"
                     /translation="MAKIVTMGEIMLRLSTPNNEKFIQADEFDVNYGGGEANVAVSLA
                     NYGHDVEFVTAVPANPIGDCAVAALRKYNVGTKHIARSGERLGIYFLETGSAMRASNV
                     VYDRANSAIATATADEFNFDEIFKDADWFHFTGITPAVSDAAIELTEAALKAAKAHGV
                     TVSVDLNFRKKLWSSEKAQKVMTNLMQYVDVCIGNEEDAEKVLGFKPGNTDVTSGELE
                     LAGYVDIFNQMADKFGFKYIISSLRESHSASDNGWSACIMDGKTREFYHSRKYHITPI
                     VDRVGGGDSFAGGLICGLADGKDMKAALEFAVAASALKHTIPGDFNLVTRADVDNLAG
                     GDGSGRVQR"
     gene            complement(4603..5256)
                     /locus_tag="ACZPCG_11355"
     CDS             complement(4603..5256)
                     /locus_tag="ACZPCG_11355"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002600110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HIR1285681.1"
                     /translation="MKKEQVLARLKEDCLVAVVRAKNLEQGEKVIDSIVAGGINFIEI
                     CMTVDEGSPIDLIANMVEKYKDNDKVVIGAGTVLDPETARMAIMAGANYVVSPGLNLE
                     TIKMCNRYRVPMLPGVMTPTEAIAAMEAGCDVIKIFPGNIVGPAAISSFKGPLPQGEF
                     MPSGGVDVDNVDKWIKAGAYAVGTGSSLTKGAKTGDFAAVTAKAKEFVDAVAAARAK"
     gene            complement(5403..>6005)
                     /locus_tag="ACZPCG_11360"
     CDS             complement(5403..>6005)
                     /locus_tag="ACZPCG_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008716703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HIR1285682.1"
                     /translation="HVAREQKQRYTIGLTAYRVGINYTNNMDFIGTIDPVLKAFSKEV
                     GKTVFFGVRADDEIVYICKYEPENPIITTATVGTKNPIYCTSLGKAILAYSEEEERLA
                     LTERIHFRAFTERTIRDKACFLDELEKVKEKGYALDARELEEHMECVGAPVFGPDGTV
                     MGAISVSSLYKPTEDYEALGRRVCEKAAMLSRLLGYIGKR"
BASE COUNT         1532 a         1527 c         1305 g         1641 t
ORIGIN      
        1 cgcgatcttc tctccggcat tattgtcatg acagccctct attccatcaa tctgaggatc
       61 acgggaaaag cgaatcttcc gattttcagc aaggaaacta ttttttcaaa tgaatttctc
      121 tccgcaaacg taccggaagc ttttaagccc tataccacca cactgattct tctggtactg
      181 gccgtgctct gcaaggtgct gctggacgcc tatttaaaaa caagatccgg ctatctgctc
      241 cgggcggtag gcgacaacga gacattagtc acttcgcttg ctaaggacaa ggggctggta
      301 aaaatcatcg gtctggcaat tgccaatggc tttgcagccc tggccggctg cgtatactgc
      361 cagcagaaag gcttttttga aatctctgcc ggcacaggaa ccatggtgat cggtctggca
      421 aatgtaatca tcggaacaca gctttttaag cggtttggct ttgtaaaatc caccacagcc
      481 gttatcatcg gctctatcct ctataaggcc tgtgtctctg ctgctcttct tgtaggcgat
      541 ctgaagattg ccggcctgaa cttgtctgtc ccggttactg cttctgattt aaaactgatc
      601 acctccgtac tgtttttgat catccttgta ttaagccctg cgggcggaaa gaaggtaaag
      661 agccatgctt gagctgaaac acatccataa atactacaat acgggtacgg tcaacgaaat
      721 gtgcctgttt gaagacttta acctgacaat ccgggatcac gaatttgtat ccgttgtagg
      781 aagcaatggt tcaggaaaga cttccctttt aaatatcatc tgcggcagta tccctttaga
      841 tgaagggacg atccgcatcg gcaatgagga tattactaaa atggcagaat acaaacgcca
      901 gcgccgcatc ggccgtgtct accagaatcc ggctatgggc acctgcccct ctatgacgat
      961 tgcagaaaac atggcattgg cagacaataa gggcaaattt tttaatctgc gccccggaaa
     1021 aaataaacag cggatcgatt tttaccgtga aagcctccgc acactgggtc tgggactgga
     1081 agataagatg gatgtaaagg taggcgttct ctccggcggc cagcgccagg ccatggcgct
     1141 gcttatgtcc accatgactc cgatcgaatt tctgatcctg gatgagcaca cggctgcact
     1201 ggacccaaaa accgcagaaa ccatcatgga gctcacagac cgcattgtaa gggaaaagaa
     1261 tctgactacc attatggtaa cccacaatct gcgctatgca gtggactacg gaaaccgcct
     1321 gctgatgatg catcagggca aagccattat ggacaaagcg ggaacagaaa aagaagaaac
     1381 aagaattgat gatattctga aaaaatttaa tgaaatcagc atagagtgcg gaaattagta
     1441 aaaaaagaat caggcagccg gccatttccg gcaaagcctg attctttttt atgggattat
     1501 tctgcaaata ccatgatttt tgtggctgta ttgtcatctc ccgaccatac gaccaccttt
     1561 ttaccctggg tcagatcatc cagagttaca atatttcttg taagatatgg gaaaatgtca
     1621 cagtcctctg ccagaatgta ctctgtgcca tctgaagccg taagcaccgt ctgggagctg
     1681 cctgcatctg taatgacaga gtctgcctcc acataatcag gtaccttaaa atccgcagga
     1741 atattggcaa aaatgatagc agcattggtc atgggcggaa ggctcatcgt catagccgga
     1801 ccaatgtacg catagatcgt ctccccatcc tggatatcct ctaatgtaag gggcgtacca
     1861 gaaactgcat ccaggatcag ggtggattcc tgagagatat ttaggatgat ctctccctca
     1921 gcgctcacgc cggaacggtt atcaatggaa agacgcccgt cctccatatg ggatgccgaa
     1981 ccgtaaatcc gtacaggctg cagctcctcg tttggagttt cttccatgct ttcaatgggg
     2041 cccgttaccg cttccactac tgtggcggcc tcagcagact ctggctgaga tgcatctgcc
     2101 tgagtctggg cactttctgc ttttgtattc atggcacagc cgctgatccc gatcaccgct
     2161 gccgcgcaaa ttgctgctac gcaaaaatag tatcttctca atgtaatcac tcctttaatc
     2221 acggtttccc gccggttaca ttttgaagta tatactattt ttcccaaaaa tccaataaag
     2281 taattcttaa catttctgac gtaagaatta aactaccgtc ttctctccta tcttgccatc
     2341 tcgctctgag gaccggactg gttatgagct ttgatgatgg tctggatctc ttccagatta
     2401 gaggacggaa gatctcccgg aatggtattc tttactgctg cggtagcatt tccgaaggca
     2461 atggcctttg agcagtcgcc gccgctgctt aaaagcccgt aaagcgcacc agaaatataa
     2521 gcgtctccgc ttccgatccg atctaccacc tcgatatccc tatatggctc ctcctcataa
     2581 taggtatctc tggcagcatc atagattaca gaaccgaagg tatgtctctt ggggctgtgt
     2641 acaattcgct gagtggatgc cacaatggaa atcggatact cctccgtaaa gctcttcatc
     2701 atctcttttg ctgtgcctgt ctttaagaag gtaaggcgtg ctgtatcctc agaacagaaa
     2761 aagatatcaa catacggaag gatcttaagg atacactctc tggcctcatc tccgctccat
     2821 aagttgccgc ggaagtttac gtcaaaggaa accagggttc ctgcttcttt aaaacgcttg
     2881 atcatctcaa tggttgtctc acggatctgc gggcttaaag caagggtgat gcctgtggtg
     2941 tggaaacatt tggaggcagt atagacctct tttggaatct cgtcaatatt gaggctgaaa
     3001 aaggaactgt tcatacggtc atagatcacc tttggttttc tgggatatgc tccattttca
     3061 tagtaataaa tgccaacgcg ggcagaggga gaatcatcat acataaaaaa gtcatcgctg
     3121 acgccaaagg aacggatctt tcccttcata aagcttccaa tatcatggga gggaagcttg
     3181 gagataacac cagtgcgaag tcccagcaag gaagcaccta ccgccacgtt taattctgct
     3241 ccgcctacct gctttacaaa ggtatcacct cttgaaaggc ggtcgttatc cggcggagaa
     3301 agccttaaaa gaagctgtcc aagggtcaga agatcaaagc ttctactcgt atattctgcc
     3361 atatttgtat cctcctaaat atttttcatt aaatgacagg gagcctgtct gaaaatccat
     3421 ctttgaaacg caattttcaa acaggctccg ctgtttacag agtatatatt gtgttcgtaa
     3481 agtgattatc tctgaacacg gccagatccg tcgccgcctg ccagattatc aacatctgca
     3541 cgggtaacca ggttgaagtc gccaggaatg gtgtgcttta atgcggaagc tgctactgca
     3601 aactccagag ctgccttcat atccttgccg tcagccagac cgcagatcag accgcctgcg
     3661 aaggagtcgc cgccgcctac acggtcaacg attggagtga tatggtactt tctggagtga
     3721 tagaactcac gggtctttcc atccatgatg catgcagacc agccattatc ggaagcgctg
     3781 tggctttcac gcagagagct gatgatatac ttgaagccaa acttatcagc catctgattg
     3841 aagatatcaa catagcctgc cagctccagc tcgccggagg taacatctgt gttgcctggc
     3901 ttgaatccaa gaaccttctc agcatcctct tcgttgccaa tgcaaacgtc tacatactgc
     3961 atcaggttag tcataacctt ctgagccttc tcggaggacc acagcttctt acggaagttt
     4021 aagtctacgg aaacagttac gccgtgagcc ttagctgcct ttaaagctgc ctctgtcagc
     4081 tcgattgctg catcgctgac agctggagta atacctgtga aatggaacca gtcagcatcc
     4141 ttgaaaatct catcaaagtt gaactcatct gctgtagctg tagcgatcgc actgtttgca
     4201 cggtcataaa ctacattgga agctctcatt gcagaaccgg tctctaagaa gtagataccc
     4261 agtctctcgc cgcttcttgc gatatgcttt gtacctacat tgtactttct taaagctgct
     4321 actgcacagt caccaatcgg atttgccggt actgctgtaa caaactcaac atcatggcca
     4381 tagttagcca gagatactgc tacgttagcc tcgccgccgc cgtagttaac atcaaactcg
     4441 tctgcctgga taaatttctc attattcggg gtagataatc ttaacatgat ctcgcccatg
     4501 gtaacgatct ttgccatttt tataatctcc ttattctctt actctatctg cgccgcccgg
     4561 atcaaccggg cggtggcttc tttgtctcag cgaaatgatt tcttatttag ctcttgctgc
     4621 agctactgcg tctacaaact ccttagcctt tgcggtaaca gctgcgaaat cgccggtctt
     4681 agcgcccttt gttaagctgc ttccggttcc aactgcgtat gcgcctgcct tgatccactt
     4741 gtcaacgtta tcaacatcaa cgccgccgga tggcataaac tcaccctgag gaaggggacc
     4801 cttgaaggag ctgattgctg ccggtcctac gatattgcct gggaagatct tgattacgtc
     4861 gcatcctgcc tccatagctg caatagcctc ggttggagtc ataacgcctg gcagcatagg
     4921 tactctgtag cggttgcaca tcttgattgt ctcaaggttt aagcctgggc ttactacata
     4981 gttagcacct gccatgatag ccattcttgc agtctcagga tccagaacag tacctgcgcc
     5041 gattactacc ttatcattat ccttatattt ctctaccata tttgcgatca gatcaatggg
     5101 gctgccctca tcaacggtca tacagatctc gatgaagttg atgccgccgg ctacgataga
     5161 atcgatcacc ttctcgccct gctccaggtt ctttgcacga accacagcta ccagacagtc
     5221 ttctttcagt cttgccagaa cttgttcttt tttcatttct cttctccttc tttcatcttt
     5281 gttcgtaaat acgaatatac ttcatattta cgatttttta taattaaatt ctaatagtta
     5341 ccgcatgatt tgtcaacagg aaaccagcga aaaaatgtac atttttacac gcaatccgcc
     5401 cattatcttt tacctatata tcccaaaagc cttgaaagca tggcggcctt ttcacagact
     5461 cttcgtccca gcgcttcata atcctctgtt ggcttataaa ggctggaaac gctgatcgct
     5521 cccataaccg ttccatccgg gccaaaaacc ggagcgccga cacattccat atgctcctcc
     5581 agttctctgg cgtccagggc atagcccttt tcctttactt tttccagttc atccagaaaa
     5641 caggccttat cccggatggt acgttctgta aatgccctga aatggatccg ctccgtaaga
     5701 gccagccgct cctcctcttc tgaataagcc aggattgctt ttccaagtga ggtgcagtaa
     5761 atagggtttt tagtgcctac cgtggctgtt gtgataatgg gattttccgg ctcatattta
     5821 cagatatata cgatctcgtc atccgcccgg acgccgaaga atacggtctt tcccacttct
     5881 tttgaaaaag cctttaaaac cgggtctatg gttccgataa aatccatatt gttggtataa
     5941 ttgattccta cccgataggc ggtaagccct atggtataac gctgcttctg ctctctggcc
     6001 acatg
//
LOCUS       DBMDUH010000160         5878 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_5409_length_5878_cov_4.034620, whole genome shotgun sequence.
ACCESSION   DBMDUH010000160 DBMDUH010000000
VERSION     DBMDUH010000160.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 5878)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5878)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5878
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5409_length_5878_cov_4.034620"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1629
                     /gene="nikA"
                     /locus_tag="ACZPCG_11365"
     CDS             <1..1629
                     /gene="nikA"
                     /locus_tag="ACZPCG_11365"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR1285683.1"
                     /translation="VSTMKKRFPALVLGGILALGLLSGCGQKEAASSVSTAASEPAAA
                     VNGSGQELVFVNYRDIRDLNPHLYAGEMYAQSILYDTLVSITEDGYEGCLAESWTISE
                     DGKIYTFKIRDGVTFSDGTVCDANAILANFNAIIENKNRHTWLEMMNLLVGVSAPDDH
                     TFVIELSEAYYPMLTELGCIRPFAMISPNCMIDGSTKDGVSGYIGTGPYVLTDFETDQ
                     YAVFERNENYWGEKPEIERITVKVIPDNQTRIMALESGEIDLIFGKNMLDADAISQYV
                     DSDKFEVALSNPTSTRHIVLNTTNEILSDTAVRQALQHATNRTAISEGIFYGLEQPAD
                     TLYAATVPYCDVELTPYEYSTETASSMLDEAGWIMGSSGIREKGGKKLELDLLYNSDS
                     VTEKTISEYLQSEYLKLGISLNIHGEEEQSYRDNMKAGNFDMVFNICWGMPYDPQSSL
                     AAMRAPVYGDFAAQQGLADKKEIDQAITDILTTTDEEERQELYRFVLTRLHEDAVYIP
                     LTFECNKALYTKNLQGVHFGTDQYDVPFSDMYFE"
     gene            1709..2647
                     /gene="opp1B"
                     /locus_tag="ACZPCG_11370"
     CDS             1709..2647
                     /gene="opp1B"
                     /locus_tag="ACZPCG_11370"
                     /inference="COORDINATES: protein motif:HMM:NF045469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt ABC transporter permease"
                     /protein_id="HIR1285684.1"
                     /translation="MKRYVIRRLMMAVPLLLAISFICFLFINLIPSNPAEVALRVQQM
                     PVITEEAIARMEEMLGLNKPFLIRYFDWLAGCLQGDFGISYVNPKRTVAGEILRCLPA
                     TLQLAGASFVIVLLLSIPIGFLCAVYKDGWFDKMMRSIVFVTTAMPPYWVGLLLMWAI
                     GVKFGWLPTNGNGTWKHLILPAFTVALSYISTYIRLIRNNMLENMKQDYVLYANVRGL
                     SPKSIWVKHVLKNSMHTCIVAMGMSIPQLISGTIVVENVFSWPGLGTLCISSIFNRDY
                     PVIQTYVLLIGVLFVVFNLIFDILQTVSDPRLRKED"
     gene            2653..3477
                     /gene="opp1C"
                     /locus_tag="ACZPCG_11375"
     CDS             2653..3477
                     /gene="opp1C"
                     /locus_tag="ACZPCG_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt ABC transporter permease"
                     /protein_id="HIR1285685.1"
                     /translation="MAKRFFQNRTAVFTTFAVLLIALIGIFAPVIAPNDPYETNIINK
                     FASYSVQYPLGTDQLGRCVLSRMIFGIRPTLGLAALTMAGTIGIGAVLGIIAGYFRGI
                     AEEIIMRIVDVMLSFPSQIMVFAVVALLGVSVQNVIIANVFIKWAWYARMIRTGVMQY
                     RDKNFVQFSRCVGMPERFILFRHLLPSITSDLAVLASLDIGWAIINISTLSFLGLGVQ
                     APTPEWGAMLNEAKEVLTRNPTQMLAPGIAIVALVCCFNLLGDAMRDVLDPKEVAK"
     gene            3474..4313
                     /locus_tag="ACZPCG_11380"
     CDS             3474..4313
                     /locus_tag="ACZPCG_11380"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1285686.1"
                     /translation="MTPVFELNHLQVSLRGKKRNTDLVKGVSFSVKQGECLGILGESG
                     SGKSMSIKAAMGLLDKNFVVSGSAKFQGDELVGKNAEQLRRLRGGQVGIVLQNPMTCF
                     DPLYRIGNQIAETFAAHQKWDDGEIKERSIEMLDKMQIRNPEEALEKYPHQLSGGMLQ
                     RIMIGIAMSMEPSLLIADEPTTAIDAITQFDILNEFAAIKQKHKTAMIFISHDLNAVS
                     QIADKIVVLNQGTVVDQGDFHHIIHHAADPYTRLLIEKRTDVMRKYRQALEGGECSHA
                     QAG"
     gene            4297..5091
                     /locus_tag="ACZPCG_11385"
     CDS             4297..5091
                     /locus_tag="ACZPCG_11385"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1285687.1"
                     /translation="MLKLDKICVSFRRECRDKLFGHTKQQVLFDVSAEVKKGTCLGIL
                     GESGSGKSTMGRVLCGLLKPDSGTVFLNGVSVYGSKQGRKNLQDRLSIVFQDYTTSAN
                     PRFRVADILKEGLFVRQRRERKKLNQEKEIRRLLELVGLSYDFAGRFPHELSGGQLQR
                     ICIARAVACNPELILFDEAISSLDAHTQVQIMDLLLDLKEKLGLTYIFITHDLTSITY
                     LCDDVLFLYQGRVTEYLPVKQLSEAKDIYARKLLESIIVFDTEEAS"
     gene            5088..>5878
                     /locus_tag="ACZPCG_11390"
     CDS             5088..>5878
                     /locus_tag="ACZPCG_11390"
                     /EC_number="3.4.17.-"
                     /inference="COORDINATES: protein motif:HMM:NF048461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HIR1285688.1"
                     /translation="MIVCGYEIQAGEKKHIKIPVLQAEAMEGICICGKNQGKTLVITA
                     GVHGCEYVGIEAAKRMAEFLDPMELAGNVILIPLVNRCGFFDGRKQVVPEDEKNLNRV
                     FPGKSNGTISERIAYTMEEYIYPCVDFLIDLHSGDSNEDLIPLVFYPTKGEKAVNDKA
                     LQAAKVLSVPYRVSSQAKNGLYSWAVQKGIPSLLIERGSGGLWSENEVERCMEDMHRI
                     MAFLGIKQGSYEKTSQADIKEAVYEEAVSDGFWYPSITAGKRVCCG"
BASE COUNT         1633 a         1122 c         1522 g         1601 t
ORIGIN      
        1 gtaagcacta tgaaaaaaag atttccggca ctggttctgg gaggaatcct ggcgcttggt
       61 cttttaagcg gatgcggaca gaaagaagct gcatcatcgg tctcaacagc agcatcggaa
      121 ccggccgctg cagtcaacgg ttccggccag gaactggttt ttgtaaatta tcgggatatc
      181 cgggatttaa atccacatct gtatgcagga gaaatgtatg cccagagcat tttgtacgat
      241 actctggtga gcattacaga agacggatat gaaggatgcc tggcagaaag ctggaccatc
      301 agcgaggatg gaaaaatata tacatttaaa atccgcgatg gagttacttt ttcagatgga
      361 acagtctgcg atgccaatgc aatcttagcg aattttaatg caattattga gaataaaaat
      421 cgtcatacat ggctggagat gatgaacctg ctggttgggg tatcggctcc ggatgaccat
      481 acatttgtaa tagaattgag tgaagcctat tatccaatgc ttacggaatt aggctgtatc
      541 cgtccttttg ccatgatttc tcccaattgc atgattgatg gaagtacaaa agacggagtg
      601 agcgggtata tcggaaccgg cccttatgtg cttactgatt tcgagacaga tcaatatgcc
      661 gtatttgagc gcaatgaaaa ttactgggga gaaaagcctg aaattgaaag gattaccgta
      721 aaagtaattc ctgataacca gacccgtatt atggcgctgg agtctggaga gatcgatctg
      781 atttttggta aaaatatgct ggatgcagat gccatcagtc agtatgtgga cagtgataaa
      841 tttgaagttg ccctttccaa tcctacctct acaagacata ttgtattaaa cacgacgaat
      901 gagattctca gtgatacggc tgtccgtcag gcactgcagc atgcgaccaa cagaacggca
      961 atttcggaag gtatttttta tgggctggag cagccggcag atacgcttta cgcagctacg
     1021 gttccttatt gtgatgtaga actgacacca tacgaatata gtacagaaac cgcatccagt
     1081 atgctggatg aagcaggatg gattatggga agcagtggca tcagggagaa aggcggaaaa
     1141 aaactggaac tggatttatt gtataacagc gatagtgtaa cggaaaaaac catttctgaa
     1201 tatctgcaga gcgaatacct gaagcttgga atttccctca acatacatgg tgaggaggag
     1261 cagtcctaca gggacaatat gaaagcaggt aattttgata tggtattcaa catctgctgg
     1321 ggtatgcctt acgatccaca gtcttcgctg gcggcaatga gagcaccggt ttacggagat
     1381 tttgcggcac agcagggact ggcagataag aaagaaattg atcaggcaat tacggatatc
     1441 ctgactacca cagatgaaga ggaacgtcag gaactgtacc ggttcgttct cacccgtctc
     1501 cacgaggatg ccgtatatat tcctctgact tttgaatgca acaaggcgct ttatacaaag
     1561 aatttacaag gcgttcattt tggaacagac cagtatgatg ttccgttttc tgacatgtat
     1621 tttgaataat caggaaaagc cgtttcttct gagaagcggc tttttaaaag ataagacctg
     1681 aaaatctata agaaagaggg gaaaagctgt gaaacgatat gtcattcgcc gtctgatgat
     1741 ggctgttcca ttgctacttg cgatttcctt tatctgtttt ctgtttatca atctgattcc
     1801 atctaatccc gcagaggttg ccctaagggt acagcaaatg cctgtgataa cagaagaggc
     1861 cattgccagg atggaggaaa tgcttggtct gaataagccg tttttgatcc ggtattttga
     1921 ctggcttgca ggatgcctgc agggagactt tggaatcagc tatgtgaatc ccaaaagaac
     1981 tgtagccgga gaaattttaa gatgtcttcc ggccaccctg cagcttgcgg gagcttcctt
     2041 tgtgatcgta ttgcttttga gtattccaat cggatttttg tgtgcagtat acaaggatgg
     2101 atggtttgat aagatgatga gaagcattgt ctttgttacc actgccatgc cgccttactg
     2161 ggttggactg ctgctgatgt gggcaatcgg tgtaaaattt ggatggctgc ctaccaatgg
     2221 aaacggaaca tggaagcatt tgatccttcc tgcatttaca gtggcattaa gctatatatc
     2281 gacctatatc cggctgatac gcaataatat gctggagaat atgaaacagg attatgtcct
     2341 ttatgccaat gtcagaggat tatctccaaa atcgatctgg gtaaagcatg ttctgaaaaa
     2401 ttccatgcat acctgcattg ttgcaatggg aatgagtatc cctcagctga tatcaggtac
     2461 cattgttgta gaaaatgtat tttcatggcc gggacttgga actctgtgca tcagttctat
     2521 ttttaacagg gattatccag ttattcagac ctatgttttg ctgatcggtg ttctttttgt
     2581 agtatttaat ttgatatttg atattttgca gacagtgtct gacccacggc tgagaaagga
     2641 ggattagaga acatggcaaa gcgttttttt cagaacagaa cggcagtttt tacaacgttt
     2701 gcagttttgc tcattgctct tattgggatt tttgctccgg ttattgcccc gaacgatccc
     2761 tatgaaacca atattatcaa taagtttgct tcctacagcg tccaataccc ccttggaacg
     2821 gatcagctgg gcagatgtgt gctttcccgc atgatctttg gaatccgccc caccttgggg
     2881 ctggcagctc ttactatggc aggaaccatt gggattgggg cagtcctggg cattatagcc
     2941 ggatatttca gagggattgc agaggaaatt attatgcgga tcgtagatgt gatgctctcc
     3001 tttcccagcc agatcatggt atttgcagtg gtggccctgc tgggagtcag tgtccagaat
     3061 gtgatcattg ccaatgtgtt tataaaatgg gcctggtatg cccgcatgat ccgtaccgga
     3121 gttatgcagt acagggataa aaattttgta cagttttcca ggtgcgtagg catgccagag
     3181 cgttttatcc tgttccgtca tctgctgccg tccatcactt ctgatctggc agttctggct
     3241 tctctggaca ttggatgggc gattattaac atctctacat tatctttttt gggactggga
     3301 gttcaggcac ccacaccaga atggggcgct atgttaaatg aggctaagga ggttctgacc
     3361 agaaatccaa ctcagatgct tgcgccagga attgcaatcg ttgcccttgt gtgctgcttt
     3421 aatctgctgg gagatgcgat gcgggacgtt cttgatccaa aggaggtggc aaagtgacac
     3481 cagtatttga attgaatcat cttcaggtat ccctgcgagg caaaaaaagg aatacggatt
     3541 tagtgaaagg cgtctctttt tcggtaaagc agggggagtg cctgggaatt ttgggggaat
     3601 caggaagcgg aaaatccatg tcaattaagg cagccatggg gcttcttgat aagaattttg
     3661 ttgtttccgg aagcgcgaaa tttcagggag atgagctggt tgggaaaaat gcagagcagc
     3721 tgcgcaggct gagaggcggg caggttggaa tcgtactgca aaatcccatg acctgtttcg
     3781 atcctctgta ccgtatcgga aaccagattg cagaaacatt tgcagctcat caaaaatggg
     3841 atgacggaga aataaaagag agatccattg aaatgttaga taaaatgcag atccgaaatc
     3901 cagaggaggc actggaaaaa tatccacacc agctttccgg cggaatgctg cagcgtatta
     3961 tgattggaat tgccatgtcc atggagccgt ctctgctgat tgcagatgag cctaccactg
     4021 caatcgatgc tattacacag tttgatattt taaatgaatt tgcagccatt aaacaaaagc
     4081 ataagaccgc gatgatcttt atttctcacg accttaatgc tgtttcgcag atagcagata
     4141 aaattgtggt tctgaaccag ggaacagtag tggatcaggg ggatttccat cacattatcc
     4201 accatgcggc agatccgtat acaaggctgt tgattgaaaa acggacggat gttatgagaa
     4261 aatacagaca ggcgctggaa ggaggagagt gcagtcatgc tcaagctgga taagatatgc
     4321 gtcagcttcc gcagggaatg ccgggacaag ctgtttggac atacaaaaca gcaggtttta
     4381 tttgatgtat cagcggaggt gaaaaaagga acctgcctgg ggattctggg tgaatccgga
     4441 agtggaaaat ctactatggg cagggtgtta tgcggacttt taaaaccgga ttcgggaacc
     4501 gtattcctca atggagtttc cgtatacggt tcaaaacagg gacgtaagaa tctgcaggat
     4561 cggttaagta ttgtttttca ggactataca acctcagcca atccccgttt cagagtggcg
     4621 gatattttga aggaaggcct ctttgtccgt caaagacgag agaggaaaaa gctgaatcag
     4681 gaaaaggaga tccgccggct gcttgagctg gtaggcctgt cctatgactt tgccggtcgg
     4741 tttccccatg aactgtcagg aggccagctt cagcggatct gcattgcccg ggcggttgcg
     4801 tgtaatccgg agttgatctt atttgatgag gcgatttcct cactagatgc ccatacccag
     4861 gttcagatca tggatctttt gcttgattta aaggaaaagc ttggattgac ctatattttc
     4921 attactcatg atcttacatc tataacctat ctttgtgatg atgtgctgtt tttgtaccag
     4981 ggaagggtga cagagtatct tccggtaaag cagctaagcg aagcaaagga tatctatgcc
     5041 agaaagcttt tagaatctat tatcgttttt gacacagagg aggcatcatg atcgtttgcg
     5101 gatacgagat acaggcgggg gaaaagaaac atataaaaat tcccgtctta caggcggagg
     5161 caatggaagg aatttgtatc tgtggaaaaa atcaggggaa aactctggtc ataacggcag
     5221 gagttcacgg ctgcgagtat gtggggatag aagcagcgaa gcgaatggct gagtttcttg
     5281 accccatgga attggcggga aacgtgatat tgatccctct cgtaaacaga tgcggatttt
     5341 ttgacggcag aaagcaggtg gttccggagg atgaaaaaaa tttaaacagg gttttccctg
     5401 gaaaatcgaa tggaacgatt tctgaacgga ttgcttatac catggaagaa tatatctatc
     5461 cctgtgtaga ttttctgatt gatcttcaca gcggagacag caatgaagat ttgattcctc
     5521 ttgtttttta tcccacaaag ggagagaagg cggttaatga taaagcatta caggcagcca
     5581 aagtattatc cgtaccttac agggtaagct ctcaggcgaa aaatggatta tacagctggg
     5641 ccgtacagaa ggggattccg tctcttttga tcgaacgggg ttcaggcggt ctgtggtcgg
     5701 agaatgaagt ggagcggtgt atggaggata tgcaccgcat tatggcattt cttgggataa
     5761 aacagggttc atatgaaaaa acatcgcagg cagatataaa agaagccgtt tatgaggagg
     5821 cagtctctga cggtttttgg tatccctcta ttacagcggg gaagcgggta tgctgtgg
//
LOCUS       DBMDUH010000161         5845 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_5437_length_5845_cov_7.726149, whole genome shotgun sequence.
ACCESSION   DBMDUH010000161 DBMDUH010000000
VERSION     DBMDUH010000161.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 5845)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5845)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5845
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5437_length_5845_cov_7.726149"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            165..635
                     /locus_tag="ACZPCG_11395"
     CDS             165..635
                     /locus_tag="ACZPCG_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AfsR/SARP family transcriptional regulator"
                     /protein_id="HIR1285689.1"
                     /translation="MYRKISLVDAEKVYIKTFGRFDLYYRGKEIDFSNAKAKELLAVL
                     VDQGGREISLHEMAQILSDGEDDETARQAVHVAWSRLKKTLVSYGLGDIVRKGRGFYA
                     LNRRRIRCDCWEMLEENEDRYFMGEYMPEYSWAEYLQSFFWKLCKKNGTKIKTL"
     gene            780..1304
                     /locus_tag="ACZPCG_11400"
     CDS             780..1304
                     /locus_tag="ACZPCG_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AfsR/SARP family transcriptional regulator"
                     /protein_id="HIR1285690.1"
                     /translation="MSQREGQNIFIKTFGGFDVFVDGKQVHFSSEKAKEMLAVLVDKR
                     GSSVSLSQMAWLLYEQSPEQTAKNNLRVIYYRLKKILENNGIGGMLIKKRGSFAVDAS
                     SFICDVYEFVEGNPDYVPLFLGNYMSGYEWGQEVVPYLNNLYCQYCETPMNGLSDQVH
                     ETILSQGKKGGKRK"
     gene            1301..2023
                     /locus_tag="ACZPCG_11405"
     CDS             1301..2023
                     /locus_tag="ACZPCG_11405"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HIR1285691.1"
                     /translation="MTDQLEMIDIHAHILPGIDDGARNWEETGRLLEAAWAQGVRHII
                     ATPHFSRKTDMEQLRQLKAGVRELAHRKGLELEISLGQELRYFEELPLYLEQGRALTL
                     AESRYALVEFKPGDGFQTIRRAVRELVQYGFIPVLAHAERYLCLRETGRTEELVQSGA
                     CLQVNAESLTGGLFDRTAAWCLKEIQKGRIHFAASDMHRPDTRPPRLEEAFKKLSKKR
                     SCPDAATIFGKNQRYILENKIL"
     gene            2060..2806
                     /locus_tag="ACZPCG_11410"
     CDS             2060..2806
                     /locus_tag="ACZPCG_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0061097 - regulation of protein tyrosine
                     kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YveK family protein"
                     /protein_id="HIR1285692.1"
                     /translation="MEKKFDDNDEIQIDLLELAYALKKKLWLIILGLIVGAFGAGLYS
                     KILLTPIYKSTAMVYVLSKETTLTSLADLQIGSQLTKDYSVLVTSRPVLEQVIEKQQL
                     DMRVDELERMISIGNPTDTRVLTITISDTDPIRAQALAEEVAKTSSDYIGDIMEMVPP
                     KIIEEGVPAEKPSSPNVKKNAAMGGLAAAVLISGIICLGVIMDDTIKTEEDVEKYLGL
                     TVLASIPDVDETGKSENEKGRKKKKTKGAV"
     gene            2810..3571
                     /locus_tag="ACZPCG_11415"
     CDS             2810..3571
                     /locus_tag="ACZPCG_11415"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HIR1285693.1"
                     /translation="MEMRLELKNTERNDYYYEEAIKTLRTNIQFCGSSLKTILFTSTV
                     SGEGKSATTVAVASSLGSIGKRVLLVDADIRKSVLVKRHEISGRPNGLSQYLSGQKKL
                     GEVIYHTNMENVDMILAGPYSPNPAELLEDELFKVLLENARELYDYIIIDTPPMLNLI
                     DGAIIANQCDGAVIVVESGGISYRMAQKVKSQLEKSGCRILGAVLNRVGGEYGSGYYK
                     KYYGKYYKKYYGKYGKYERYGYDSPKERENIEESR"
     gene            3555..4604
                     /locus_tag="ACZPCG_11420"
     CDS             3555..4604
                     /locus_tag="ACZPCG_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIR1285694.1"
                     /translation="MKKADKKKREWLVTAAVILAILFAAVSWNLYQEKKLAAAEAQLW
                     REQAGTSQKEGKAGYGQVQNTIEYKGKTYRRNTYVKAILCMGIDRREPLTETKTAGAG
                     GQSDGIFLVAQDTARDRVQVLAIPRDTMTPIPLTDLSGNILGYDTQHLTLAYAYGDGR
                     EKSCQFMCQAVTELLDGLQIDGYMAVSMGALKPLNDAVDGVTVTIDDPELEKRDPAFK
                     PGTSVTLMGDQAEAFLRYRDTRKSQSAVARLERQKGYIAGYAEAVKKKASREEGVALK
                     LLETAAPYMVTNLSKDQYLDMVMAFIGSSQQLGGDDIRTLPGEGAETRIYDEYHPDPE
                     GVTQMVLDLFYRVEE"
     gene            4639..5319
                     /locus_tag="ACZPCG_11425"
     CDS             4639..5319
                     /locus_tag="ACZPCG_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285695.1"
                     /translation="MKKKAVVIAMAAALAVSQYAAVFAANSPGTGVVIPNEDSDGDYD
                     IGERVDQVTGGSSSASGTGNSSAATPGGQTAAQITVPTESGTASQSGGQAVVGDTGIE
                     FVQGSESAVSGLPETVVSTINAINTGTGLAEAGTGLDLSGYNALVGTTAVMTYQAGTR
                     VEKTGATNMPVYVPNLVEGLGTVQVLFYNNMTSKWELITPTSVDVAKKMIHVTIPNSG
                     TFSVIYKR"
     gene            5438..5650
                     /locus_tag="ACZPCG_11430"
     CDS             5438..5650
                     /locus_tag="ACZPCG_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285696.1"
                     /translation="MRKMMECLMVVLMVWSVFQIRNCITLQDVQYAQDRIHWDILAYE
                     LLFLASCFGFVTAMGTGILKKNHSEE"
     gene            5657..>5845
                     /locus_tag="ACZPCG_11435"
     CDS             5657..>5845
                     /locus_tag="ACZPCG_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HIR1285697.1"
                     /translation="MLIRNKSFYMFADAIAVVVSFILALLGRFASLDAGDFQLGNWYG
                     CILMLFIYIVVILFYQPDQ"
BASE COUNT         1762 a         1196 c         1607 g         1280 t
ORIGIN      
        1 ctttgaatga agaattgtaa agtacttctg caactgtcgc aaaatttgtg acagttcaaa
       61 aattagtaat ttaactatct ttttactgga aaaacacctc tttctgtgtt agtaaaaaaa
      121 tatgttcttg cttccggaaa taccgggggg ggggtggtat ataaatgtac cgtaaaataa
      181 gtttagtgga cgcagaaaag gtttacatta aaacatttgg gagatttgac ctgtattata
      241 gggggaaaga gattgatttt tcaaatgcca aagcaaaaga actgctggca gttctggtag
      301 accagggtgg cagggaaatc agcctgcatg agatggccca gattctttcg gacggggaag
      361 atgatgaaac ggccagacag gcagttcatg tggcgtggtc caggctcaag aaaacgctcg
      421 tcagctatgg gctgggagat atcgtgagga aaggccgcgg attttatgcg ctgaaccgcc
      481 ggcggatccg gtgcgactgc tgggagatgc tggaggagaa tgaagaccgt tactttatgg
      541 gcgaatatat gccggagtac agctgggcag aatacctgca gtcctttttt tggaaattat
      601 gtaaaaaaaa cggtactaaa ataaaaacac tttgaaaatg ttacgaaaat acaggaaaca
      661 tgcataacaa tgcaaaactg taacggtact gataaggttc atggtgtatc ttattaaagg
      721 atagttaata aaaattaagt ttttattaag aatacgatta aaagcgggag agatatttaa
      781 tgagtcagag ggaagggcaa aacatattta taaaaacctt tggcgggttc gatgtatttg
      841 tagacgggaa acaagtccat ttttccagtg aaaaggcaaa ggaaatgctg gcggttctgg
      901 tggataagag agggagcagc gtatccttat cacagatggc ctggctgctg tatgaacagt
      961 caccggaaca gaccgccaaa aataatcttc gggtgattta ttaccggctg aagaaaatac
     1021 tggagaataa cgggattggc ggcatgctga tcaaaaagag aggaagtttt gcggtggacg
     1081 ccagcagctt tatctgtgac gtatacgaat ttgtggaagg gaatcccgac tatgtcccct
     1141 tgtttctggg aaactatatg tccggttacg agtgggggca ggaggtagtg ccttatttaa
     1201 acaaccttta ctgccagtac tgtgaaacgc ctatgaacgg actttccgat caggttcatg
     1261 agacgattct cagtcagggg aaaaaaggcg gaaagagaaa atgacggatc agctggaaat
     1321 gatcgatatc cacgctcata ttctgccggg aattgacgac ggagccagaa actgggagga
     1381 aaccggccgt ctgctggagg cagcctgggc gcagggagtc cgccatatta tcgcaacccc
     1441 ccatttttcc agaaagacgg atatggagca gttaagacaa ttaaaagcag gcgtccggga
     1501 gctggctcac agaaaagggc tggaacttga aataagcctg ggacaggaac tgcggtattt
     1561 tgaagaactg ccgctgtatc tggaacaggg cagagcgctc acactggcag aaagccggta
     1621 tgctctggtg gaattcaaac cgggagatgg atttcagacc ataagacggg cagtccggga
     1681 actggtccag tacggattta ttcccgtatt ggctcacgca gagcggtatc tttgccttcg
     1741 tgaaacaggc aggaccgagg agctggttca gagcggagcc tgtctgcagg tcaatgcaga
     1801 aagcctgaca ggcggtctgt ttgaccggac ggcagcctgg tgcctgaaag aaatccagaa
     1861 ggggaggatc cactttgccg ccagcgatat gcaccggccg gatacaaggc cgcccaggct
     1921 ggaagaggca tttaaaaaac tgtcaaaaaa gcgcagctgt ccagatgcgg ccaccatatt
     1981 tgggaaaaat cagagatata ttctggaaaa caagatactg tagcaaaact attatgaaaa
     2041 gaaaaggaaa tattatacca tggaaaagaa gtttgatgac aatgacgaga tacagatcga
     2101 tctgctggaa ctggcctatg ctttaaaaaa gaagctgtgg ctgatcatcc tggggctgat
     2161 cgtaggagct ttcggagcgg ggctgtacag caagatcctg ctgacgccca tttataaatc
     2221 caccgcaatg gtctatgtac tttcaaagga aacaactctt acctcactgg cagacctgca
     2281 gatcggcagc cagcttacca aggactacag cgtgctggta accagccgtc cggtattgga
     2341 gcaggtgatt gaaaaacagc agttagacat gagggtggat gaactagagc gtatgatcag
     2401 cattggaaat ccgacagata cccgtgtgct cactattacc atatcggata cagatccgat
     2461 ccgcgcccag gcgctggcag aagaggtggc aaagacttct tccgattaca taggcgatat
     2521 tatggaaatg gttccgccca agatcatcga ggaaggcgta ccggcagaaa aaccaagttc
     2581 tccaaatgtg aaaaagaatg cggctatggg cggtctggca gcagccgtac tgatcagcgg
     2641 aattatctgt ctgggagtga tcatggacga cactataaag acagaagaag acgtggagaa
     2701 atatctggga cttactgtac tggcttctat tccggatgtg gatgaaaccg gcaaatcaga
     2761 aaacgaaaag ggaagaaaaa agaagaaaac gaagggagcg gtgtaaaaaa tggagatgag
     2821 actggaatta aaaaatacgg aacgaaatga ttattactat gaagaagcca ttaagacact
     2881 gcgtaccaat atccagttct gcggcagcag cttaaaaacc attctcttta caagtaccgt
     2941 atcgggagaa ggaaaaagcg ctactacagt agccgtagct tcttctctgg gaagtatcgg
     3001 gaaaagggtt cttctggtag acgcggatat ccgcaaatcc gtactggtga agcgtcatga
     3061 gatcagcggt cgtcccaacg gcctgtccca gtatctcagc ggccagaaaa agcttggaga
     3121 agttatttac cacacaaata tggaaaatgt ggacatgatc ctggcaggcc cctattctcc
     3181 caatccggca gagctgttag aggatgaact gttcaaagtc ctgctggaaa atgcaaggga
     3241 attgtatgat tacatcataa tcgatacccc tcccatgtta aaccttattg acggcgcgat
     3301 catcgcaaat cagtgtgacg gagctgtgat cgtagtggaa agcgggggca tcagctaccg
     3361 tatggcccag aaggtaaaat cccagctgga aaagagcgga tgccggatcc tgggggcagt
     3421 cttaaaccgg gtgggaggag agtacggctc aggctattat aagaagtatt acggcaaata
     3481 ttataagaaa tattacggaa aatacggtaa gtacgagcgt tacggatacg attccccaaa
     3541 agaaagagaa aacattgaag aaagcagata agaaaaagag agaatggctg gttacagcag
     3601 cggttattct ggcaatcctg tttgcagctg tttcctggaa tctgtatcag gaaaaaaagc
     3661 tggctgccgc cgaggcacag ctctggagag agcaggccgg tacatcacag aaagagggga
     3721 aagccggata cggacaggta caaaacacca tcgaatacaa aggaaaaacc tatcgccgga
     3781 atacctatgt aaaagccatt ctgtgcatgg ggatcgaccg gagggaacct ctgacagaga
     3841 cgaaaacagc gggagcgggg ggacagtccg acggcatctt tctggtcgcc caggacacgg
     3901 ccagagaccg ggtgcaggtt ctggccatac caagagacac catgaccccc attcccctta
     3961 cagatctgag cggaaatatc ctgggctatg atacgcagca tctcacactg gcctatgcct
     4021 atggggacgg aagagaaaaa agctgccagt ttatgtgcca agcggtgaca gagcttctgg
     4081 acggccttca gatcgacgga tacatggctg taagcatggg agcgctgaaa cctcttaatg
     4141 acgcggtaga cggcgtaacc gtaaccattg acgatccgga gctggaaaaa agagatccgg
     4201 cctttaaacc cggaacctcc gtaaccctga tgggcgatca ggccgaagct ttcctgcgct
     4261 acagggatac aagaaaaagc cagagcgccg tagcccgtct ggagcgccag aagggatata
     4321 tcgccggtta tgcggaggcc gtgaagaaaa aggcctcacg ggaagaggga gttgcattaa
     4381 agcttctgga aacggcagct ccctatatgg taacaaatct ttccaaggat cagtatctgg
     4441 atatggtgat ggcatttatt ggaagcagcc agcagctggg gggagatgat atccgcacac
     4501 ttccgggaga aggagcagag acaaggatct atgacgaata ccatccggat ccggaaggag
     4561 taacacaaat ggttctagac ctgttttaca gggttgaaga atagaacaaa tttatcagga
     4621 aaggaaaggt gaacgatcat gaagaaaaag gcagtagtta ttgcaatggc agcagctctt
     4681 gcggtttcac agtatgcagc cgtatttgcg gcaaacagtc cgggaaccgg agttgtgatt
     4741 cccaatgagg acagcgacgg cgattatgac atcggagagc gcgtggatca ggtaacaggc
     4801 ggatcttcct cagcatcagg cacaggaaat tcctcagccg ccacgccggg aggacagacc
     4861 gcagcccaga ttacggttcc cactgaaagc ggaacagcca gccagagcgg cggccaggcc
     4921 gtagtaggag atacaggcat cgagtttgta cagggaagcg aaagcgcggt aagcggactt
     4981 ccggaaaccg ttgtatccac cattaatgcc attaataccg gaaccggtct ggcagaggcc
     5041 ggaaccggtc tggacttaag cggctacaat gccctggtag gaaccaccgc agttatgacc
     5101 tatcaggccg gaaccagagt ggaaaaaaca ggagctacca atatgcctgt gtatgtccca
     5161 aatctggtag agggtctggg aaccgtacag gtgctcttct acaacaacat gacttccaag
     5221 tgggagctga tcacgcctac atctgtggat gtggcaaaga agatgatcca tgtgaccatc
     5281 ccgaactcgg gaacattttc ggttatctat aagcgctgac agagcgtgta aggataatat
     5341 gcgccggttt ccatgatcgt gaggccggcg cagtcacaaa acgctgtttt gaaaacacac
     5401 aaaggaaaag ggggacatat taatgaaatc tgcaagtatg agaaaaatga tggaatgttt
     5461 gatggttgta ctgatggtat ggtctgtatt tcagatcaga aactgtatta cattacagga
     5521 cgtccagtat gctcaggaca ggatccactg ggacatactg gcatatgaac tgctctttct
     5581 ggcgtcctgt tttggttttg tgacagctat ggggacagga attttaaaaa agaaccacag
     5641 tgaggagtaa ttgattatgc tgatccggaa caaatcattt tatatgtttg cagacgccat
     5701 tgctgtggtt gtatcattta tacttgccct gctgggcaga tttgcttctc tggatgcagg
     5761 ggacttccag ctgggaaact ggtacggctg tattttgatg ctttttattt acattgtggt
     5821 tattttattc tatcagccgg accag
//
LOCUS       DBMDUH010000162         5833 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_5447_length_5833_cov_5.402477, whole genome shotgun sequence.
ACCESSION   DBMDUH010000162 DBMDUH010000000
VERSION     DBMDUH010000162.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 5833)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5833)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5833
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5447_length_5833_cov_5.402477"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(185..1549)
                     /locus_tag="ACZPCG_11440"
     CDS             complement(185..1549)
                     /locus_tag="ACZPCG_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015851 - nucleobase transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease family protein"
                     /protein_id="HIR1285698.1"
                     /translation="MGKTAKTAGNAADCSINNIYRLEGRVPIGKAIPFGLQHILAMFV
                     SNLAPITIIAGASKTPLTPAQLGMLLQNAMFVAGIATMIQLYPIWRIGSKLPVVMGVS
                     FTFVTVLSTISANYGYPAVVGAVLVGGLFEGTLGLMAKYWRKIITPIVAASVVTAIGF
                     SLFTVGARSFGGGYNDDFGSAQNLILGTVTLVACLLWNIFAKGYLKQLSVLAGLVVGY
                     ILAITMGKVDLSMIMSGGIISLPHLMPFKPEFYPGAIISACIIFLVSAAETIGDTSAL
                     VAGGLNRKITGEEISGSLGCDGYGSVISGLFGCPPVTSFSQNVGLVNMTKVVNRFTIM
                     TGAACMILAGLLPPVGNFFASLPDAVLGGCTIMMFGTILTSGIQMVADCGFSQRNITI
                     AALSLSIGIGFTTASEVGIWDIFPPMIQSVFSANVVAVVFVVSIILSLILPKDMDVKK
                     IDQQ"
     gene            complement(1630..2121)
                     /gene="xdhC"
                     /locus_tag="ACZPCG_11445"
     CDS             complement(1630..2121)
                     /gene="xdhC"
                     /locus_tag="ACZPCG_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002197 - xanthine dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006144 - purine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase subunit XdhC"
                     /protein_id="HIR1285699.1"
                     /translation="MLKIIHMKVNGKEHELAVDERESLLDTLRLRLGLTSVKKGCEVG
                     ECGACTVLVNGEAIDSCIYLTMWADGKSVMTVEGLKGPNGELSPIQKAFIEEAAVQCG
                     FCTPGLIMSAVEIVGTGKKYNREELKKLISGHLCRCTGYENILNAMERIVEEVYQTTH
                     KEA"
     gene            complement(2114..3004)
                     /gene="xdhB"
                     /locus_tag="ACZPCG_11450"
     CDS             complement(2114..3004)
                     /gene="xdhB"
                     /locus_tag="ACZPCG_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002197 - xanthine dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006144 - purine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase subunit XdhB"
                     /protein_id="HIR1285700.1"
                     /translation="MFDIKSFYEAKDVQDAVQALVNEPDAEVISGGTDVLIRVREGKD
                     AGKALVSIHNIPELKGVNVLENGDLWIGAATAFSHITNDPLIQKLIPMLGEAVDMVGG
                     PQIRNTGTIGGNICNGATSADSASTMWTLNAKVILVGPEGERIVPICEFYTGPGRTVR
                     AREEVCTGFLIAKEDYEGWYGQYVKYGKRKAMEIATLGCAVRVKLSEDKKVIEDVRLG
                     YGVAGPTPLRCRNAEEGLKGARVDDKEAAEQFAKTALTEVNPRSSWRASKEFRLQLIE
                     ELAKRALAESITRAGGNCNA"
     gene            complement(3025..5451)
                     /gene="xdhA"
                     /locus_tag="ACZPCG_11455"
     CDS             complement(3025..5451)
                     /gene="xdhA"
                     /locus_tag="ACZPCG_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002197 - xanthine dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006144 - purine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase subunit XdhA"
                     /protein_id="HIR1285701.1"
                     /translation="MKVVGQEITRVDAFGKVTGEAKYTADLEPRDILHGKVVHSTIAN
                     GLVKSFDLDEALKVPGVVKIVTCFDVPDIQFPTAGHPWSVELKHQDICDRKLLNQRVR
                     LYGDDIAAVVAENEVAAAQAARLIKVEYEEYEPIVTVEAALKPEATPLHPDLRKDNVV
                     VHSHMTMGSSDFTFEEGRKKAIEEYGEENLVEIDETYDTPRISHCHIELPVSWSYVDT
                     NGKVTIVSSTQLPHIVRRVTAQALGIPVGKVRVIKPYIGGGFGNKQDVLYEPLNAFLS
                     MSVGGRAVRMEISREETISGTRTRHAIKGVCKGIVTKDGTVLARRLEAFANNGGYASH
                     GHAICANCGNVFKDLYKDRLGTEVDCWTVYTSSPTAGAMRAYGIPQADWFAECLTDDM
                     ADAVGMDPCEFRLKNCLDAGFVDPANGITFHTYGLTECIKKGREYIHWDEKREAYKNQ
                     TGPVRRGVGMAMFIYKTGVYPISLETASARITLNQDGSIQLSMGATEIGQGADTVFTQ
                     MAAETTGISVDRVYINSTQDTDVTPFDTGAYASRQTYVSGMACKKCALEFRQKVLEYA
                     AYMLNHDVTDLSKTVYADLVKEAAGKVRQVLGLHAEEEITWEMLDIADSQVVVNGGNP
                     VLFDVAVVADTAFYSLDHSIHITAEVTNQCKDNTFASGCCFVDLEIDMPLGLVTVKDV
                     INVHDSGILINPKTAAGQVHGGMSMSLGFGLSEELLVDEKTGKPLNNNLLDYKIPTAM
                     DAPELHVEFIQLDDPTGPYGNKSLGEPPAIPVAPAIRNAILHATGVHMNTAPMTAQRL
                     IAAFKEKGLI"
     regulatory      complement(5631..5726)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
BASE COUNT         1473 a         1569 c         1221 g         1570 t
ORIGIN      
        1 agatgattct aaaaagacct ctcagttttt tcttcatatg tatgtccctt tcttctaaaa
       61 caatggcccc atattctttc ccgagagaaa atacgcggcc agccccaaag aagctaaacc
      121 gcgtattatt atctcatata ttttcttttc tggcaagtat acgaagctgc ctccattctg
      181 gccgctactg ctgatcgatc tttttcacgt ccatatcttt tggaaggatc aggcttaaaa
      241 taatagatac tacaaacact accgctacca cattggcaga aaatacggac tggatcatcg
      301 gcgggaagat atcccagatc ccgacctcgc tggctgtggt aaaaccgatt ccgatggata
      361 aggacagcgc tgcaatggta atatttctct gagagaaacc gcagtcagcc accatctgaa
      421 taccagaggt taagatagtt ccaaacatca tgatcgtaca tccgcctaat actgcgtccg
      481 gaagggaagc aaagaaattt cccaccggcg gaagaagtcc tgctaaaatc atgcaggcag
      541 cgcccgtcat gatcgtaaaa cggttcacta cctttgtcat attgacaagg cctacgttct
      601 gtgaaaaaga agttacagga ggacatccaa agagaccgga aataacggaa ccatagccgt
      661 cacagcccag agaacctgag atttcctctc ctgtgatctt ccggtttaat ccgcctgcta
      721 ccagagcaga ggtatcgccg atggtctcag ccgcagacac caggaaaatg atacatgcgg
      781 agataatcgc tcccggataa aattccggct taaagggcat cagatggggc aaggaaatga
      841 ttccaccgga catgatcata gacagatcta cctttcccat ggtgatcgcc agaatatatc
      901 ctactaccag gccggctaaa acggaaagct gctttagata tccctttgca aaaatattcc
      961 acaaaagaca ggcaaccagt gttacggttc caaggatcag gttctgggca gaaccgaaat
     1021 catcattata gccgccgcca aaagatctgg ctcctacggt aaagagtgaa aaaccgatgg
     1081 ctgttactac agaggccgct acgatcggag taatgatctt tctccagtat tttgccatca
     1141 gtcctaaggt tccttcaaag agacctccca ccagaaccgc tcctactaca gccggatagc
     1201 cgtaattggc tgaaatggta ctgagcaccg ttacgaaggt aaaacttaca cccataacca
     1261 caggaagctt tgatccgatc cgccagatgg gataaagctg gatcatggta gcaatgcctg
     1321 ctacaaacat ggcgttctgc agaagcatac ccagctgggc cggtgtaagc ggcgtcttgg
     1381 acgctcccgc aataatggtg atcggcgcca ggtttgaaac gaacatggct aaaatatgct
     1441 gcagtccaaa gggaatagcc tttccgatgg gcactcttcc ctccagtctg tatatgttgt
     1501 taatactgca gtctgctgca tttcctgctg tttttgctgt tttccccata cgatttaccg
     1561 cctccattct catgccggca gctgtcttta tttatctccc cagaccagac atctgccggc
     1621 acactcattc tatgcttctt tatgtgtggt ctgatatact tcttccacaa tccgttccat
     1681 agcgtttaaa atgttctcat agcccgtgca gcggcacaga tgaccggaaa tgagcttttt
     1741 aagctcttcg cggttatact tctttcccgt tcccacgatc tcaaccgcac tcatgatcag
     1801 acccggcgta cagaagccgc actggacggc cgcctcctca atgaatgcct tctggatcgg
     1861 agaaagctcg ccgtttggcc cctttaagcc ttctacggtc attacgctct ttccatctgc
     1921 ccacatggtc agataaatac agctgtcaat ggcctctccg tttaccagta ccgtacaggc
     1981 tccgcactct cctacctcac agcccttttt caccgaagtc agccccaggc gcagacgcag
     2041 agtatctaaa agggactctc tctcgtccac tgccagttca tgctcttttc cgtttacttt
     2101 catatgtatg atcttaagca ttgcaattac ctcctgctct tgtgatagat tctgccagtg
     2161 cacgctttgc taactcctca atgagctgta agcggaattc cttggatgct ctccaggaag
     2221 aacggggatt tacttctgtg agcgctgttt ttgcaaactg ctctgccgcc tctttgtcat
     2281 ccactcttgc tccttttaag ccttcctctg cgttccggca gcgaaggggc gtaggtcctg
     2341 ccactccgta gcccagacgc acatcctcaa tgaccttctt gtcttctgaa agctttaccc
     2401 ggactgcaca gcctaaagtg gcaatctcca tggcctttct ctttccgtac tttacatact
     2461 ggccgtacca gccctcataa tcctcttttg caatgagaaa gcccgtacat acctcttctc
     2521 tggctcttac ggtccgtccg ggacctgtat aaaactcgca gatgggaacg attctctccc
     2581 cttccggtcc cactaggatc actttggcat ttaaggtcca catggtagaa gctgaatctg
     2641 cagaagtagc tccattgcag atatttccgc cgatggttcc tgtgttgcgg atctgaggtc
     2701 cacccaccat atccactgcc tcacccagca tggggatcag cttctggatc aggggatcat
     2761 ttgtaatatg ggaaaatgca gtagctgctc cgatccagag atctccgttt tccagaacat
     2821 ttactccctt aagctccgga atattgtgga tagatacaag ggcttttcct gcatcctttc
     2881 cctctcttac acggatcagt acgtcggtac caccgctgat cacctctgca tccggctcat
     2941 ttaccagagc ctgcacagca tcctgtacat cctttgcctc gtaaaatgat tttatatcaa
     3001 acatgcctta tttctccttc cttcttaaat aagtcccttt tctttaaacg ctgcgatcag
     3061 acgctgggct gtcataggcg ctgtattcat atgtacgccg gttgcatgaa ggatggcgtt
     3121 ccggatcgcc ggagcaaccg gaattgccgg cggctcgccc agagatttgt ttccataagg
     3181 gcctgtagga tcgtctaact ggataaattc cacatgaagc tccggagcat ccatagcggt
     3241 agggatcttg taatccagaa ggttattatt taacggtttt ccggtctttt catctactaa
     3301 aagctcctca gaaagtccaa aaccaaggct catgctcata ccgccgtgta cctgtccggc
     3361 ggctgtcttc ggattgatca ggataccgga gtcatggaca ttgatcacat cctttacagt
     3421 cacaagaccc agaggcatat cgatttccag atccacaaag cagcagccgg aagcaaaggt
     3481 attgtccttg cactggtttg taacctctgc tgtgatatgt atagagtggt ctaaggaata
     3541 gaacgctgta tcagctacta ccgccacatc aaagagtacc ggattgccgc cgtttacaac
     3601 cacctggctg tccgcaatgt ctaacatctc ccaggtaatt tcctcctctg catgaagccc
     3661 cagaacctga cgtaccttac cggcagcctc ctttacaaga tctgcataga ccgtctttga
     3721 aagatctgtc acatcatggt tcagcatata ggcagcatac tctaagacct tctgacggaa
     3781 ctctaaggca cactttttgc aggccatgcc ggatacatag gtctgacggg acgcataagc
     3841 acctgtatca aagggagtta catccgtatc ctgtgtggaa ttgatatata ccctgtctac
     3901 gctgattcca gtagtctctg ccgccatctg ggtgaatacg gtatctgctc cctgtccgat
     3961 ttccgtagcg cccatggaaa gctggatgga accgtcctga tttaaagtga tgcgggcaga
     4021 agcagtttcc agagaaatgg gataaacgcc tgttttgtaa ataaacatcg ccattcccac
     4081 acctcttctc accggtccgg tctgattctt ataggcctct ctcttttcgt cccagtgaat
     4141 gtactcccgg ccttttttaa tgcactcggt caggccatat gtatggaatg taataccgtt
     4201 ggccggatcc acaaagcctg catccagaca gttctttaag cggaattcac agggatccat
     4261 accgactgca tctgccatat catctgtcag acactctgca aaccaatctg cctgggggat
     4321 gccgtatgct ctcatggctc ctgccgtagg ggaagaggta taaacggtcc agcagtctac
     4381 ctctgtaccc agccggtctt tatacagatc cttaaataca tttccgcagt tggcacagat
     4441 agcgtgacca tgggaggcat agccgccgtt attggcaaag gcctccagcc gtcttgcaag
     4501 tacggttccg tcctttgtaa caataccctt gcacacgccc ttgatcgcat ggcgggtacg
     4561 ggttccagaa atggtttcct cgcggctgat ctccatccgg acggctcttc cgcctacact
     4621 catggaaagg aaggcattta acggctcata aagtacatcc tgcttgtttc caaaaccgcc
     4681 tccgatatag ggcttaatca cgcgaacctt accaactggg attcccaatg cctgagccgt
     4741 tacacggcgt acaatatggg gaagctgcgt ggaagaaaca atggtcactt ttccgtttgt
     4801 atccacatag gaccaggaaa cgggaagctc aatatggcag tgggaaatcc ttggagtatc
     4861 atatgtttca tcaatctcca ccagattttc ctctccgtac tcttcaatgg ctttctttct
     4921 tccctcttca aaagtaaaat cagaagatcc catggtcata tgggaatgaa cgactacgtt
     4981 gtcctttctg agatcgggat gaagaggtgt tgcctccggc tttaatgccg cttctacggt
     5041 aacaatgggt tcgtactcct cgtactcaac cttgatcaga cgagccgcct gagccgcagc
     5101 cacttcattt tcagccacaa ctgccgcaat atcatcccca taaaggcgga ctctctgatt
     5161 taaaagcttt ctgtcacaga tatcctgatg ctttaactcc acgctccagg gatgacctgc
     5221 ggtgggaaac tggatatccg gaacatcaaa acaggtgacg atctttacca ctcccggcac
     5281 ctttaacgcc tcgtccagat caaagctctt tacaaggcca tttgcaatgg tggaatggac
     5341 gacttttcca tgaaggatat ctctgggttc caaatctgcg gtatacttgg cttcaccggt
     5401 cactttaccg aaagcgtcca cgcgtgtgat ttcctggcca acaactttca ttgtatttcc
     5461 tccttacttt actgtctgtg tctgcatccc gaacgatcgt tccgattctt tcatcctcgg
     5521 ataaacggac agctctcttt ttataccaat gccgcaagca tgaatttttc aggtaaaaat
     5581 aactgttaaa atccaacaac gcccagctta taaacaggta caaaaaaaga gctgactaca
     5641 taaatgtatg tagtcaactc ttaaggtagt tggtagagac tctgggccaa tccccaggat
     5701 atacatatca gacaatttgc tttattgaat tatctatatt atataccctt aaaaaaattt
     5761 agtctatctg catttttctc aaatattttc tttattttta tgcatttttc acaaaaacaa
     5821 cctaaaaatt ttt
//
LOCUS       DBMDUH010000163         5820 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_5457_length_5820_cov_4.860990, whole genome shotgun sequence.
ACCESSION   DBMDUH010000163 DBMDUH010000000
VERSION     DBMDUH010000163.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 5820)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5820)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5820
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5457_length_5820_cov_4.860990"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(443..513)
                     /locus_tag="ACZPCG_11460"
     tRNA            complement(443..513)
                     /locus_tag="ACZPCG_11460"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(479..481),aa:Gly,seq:ccc)
     gene            complement(580..1785)
                     /locus_tag="ACZPCG_11465"
     CDS             complement(580..1785)
                     /locus_tag="ACZPCG_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1285702.1"
                     /translation="MKQTMNETKPSGWQRRTILFLTSQCITLFGSTLVQMAVVWYATM
                     ETASGAWVAAFTVCSYLPQFLISFSGGVWADRYSRKLLIAGADALTALATLAMLLAVP
                     HITSKPLFLGGLLLMSVIRSLGAGIQTPAVNAVIPQLVPEEHLMRCNGINAAMQSAVQ
                     FAAPAAAGAIFAVSTLDTTLMIDIVTAILGIGLLSCLSIPSCKSQGQCSSFIQDMKAG
                     VNYAFSHRTIGRLLILYGLFTFLCVPAGFLAGLFVRRSFGDTYWYLTAVEVIGFGGMI
                     LGGILMSLKNNGQSKTRLLSWGLFSFGAFAIGMGLSAYFIPYLIMMFFYGIALTVVQT
                     SITTLIQEHTDVSMQGRVFGLLGTMYSGFLPLGMALFGPMADAFPLSWIMIGSGGALI
                     LMGLYSKLL"
     gene            1945..2139
                     /locus_tag="ACZPCG_11470"
     CDS             1945..2139
                     /locus_tag="ACZPCG_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285703.1"
                     /translation="MYKKDICEKKKQERDCLVMDLDLVIGGRPDRVRELLAHLDFDMH
                     PPGPELKRLAEELEKKLEAQ"
     gene            2238..4157
                     /locus_tag="ACZPCG_11475"
     CDS             2238..4157
                     /locus_tag="ACZPCG_11475"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit B"
                     /protein_id="HIR1285704.1"
                     /translation="MATEQYNADSITVLEGLEAVRKRPGMYIGGVGTKGLNHLIYEIV
                     DNAVDEHLAGFCSKIWVKLEKDGSCTVKDNGRGIPVEMHQKGVSAARIVLSTLHAGGK
                     FDNEAYKTSGGLHGVGSSVVNALSEYMDVKISRGGLVYHDAYEKGRPVVELEQGLLPV
                     VGKSRESGTQINFLPDGEIFEKTRFKADWLKSRLHETAYLNPNLTIFYENHRSGEEEA
                     ITYHEPEGIISYVKELNSGKTPVHEPIYFKGEVDRVEVEAAIQFVDTFEENILGFCNN
                     IFTQEGGTHLAGFKTRFTAMINSYARELGILKEKDPNFTGADTRNGMTAVIAVKHPDP
                     IFEGQTKTKLASADATKAVFTVAGDELQLYFDRNVEVLKGVIGCAEKSAKIRRAEEKA
                     KTNMLTKSRFSFDSNGKLANCESRDASKCEIFIVEGDSAGGSAKTARNRQYQAILPIR
                     GKILNVEKASMDKVLANAEIKTMINSFGCGFSEGYGNDFDITKLRYDKIILMTDADVD
                     GSHIDTLLLTFLYRFMPELIYDGHVYIAMPPLFKVIPKKGEEQYLYDEKELERYRRTH
                     KGDFTLQRYKGLGEMDAEQLWETTLDPERRVLKRVEIEDARLASEVTEMLMGSEVGPR
                     RQFIYEHADEAEIDA"
     gene            4172..>5820
                     /locus_tag="ACZPCG_11480"
     CDS             4172..>5820
                     /locus_tag="ACZPCG_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIR1285705.1"
                     /translation="MAEKILRTEYSEEMQRSYMNYSMSVITARAIPDARDGLKPVQRR
                     VLYDMSQLRLDYDKPHRKSARIVGDTMGKYHPHGDSSIYDTLVVMSQEFKKGMALVDG
                     HGNFGSIEGDGAAAMRYTEARLEKFAQEVYLKDLDKTVNFVPNYDETEKEPEVLPVRV
                     PNLLVNGAEGIAVGMSTSIPPHNLGEVIDAVTAYIDNPAITAAELMEHMPGPDFPTGG
                     IIANKSDLPEIYESGTGKIKLRGRFEVELGKRKADKDKLVITEIPYTMIGAGINKFLV
                     DVADLVESKKLTDVVDISNQSNKDGIRIVLELRKDADIDRIKNILYKKTKLEDTFGIN
                     MLAIADGRPETLNLKGILRNFLEFQYQNTERKYNVLLEKELEKKEIQEGLISACDCID
                     LIIAILRGSKSLKDAKACLISGDVSNIHFRMPGFEEEAKKLHFTERQASAILEMRLYK
                     LIGLEILALEKEHKETLKRIESYKKILGSRGVMNRVIKDDLAAIRKEFAVPRRTRIED
                     GPEAVYVENAVPVQEVVFVMDRFGYCKLLDKSTYERNQETV"
BASE COUNT         1658 a         1211 c         1573 g         1378 t
ORIGIN      
        1 tagtttcctg aaggcttatg tttaaatgga tttttgtatc cgctaatcaa agaatagtag
       61 ctgatatgtt ccagcgtatg gattgcagtt gggacatctg aaatggttaa ttgtttttcg
      121 ttttgtaatt tttcaatttg ctggtcgtat gtaagaaatt ttttctccat atgttactcc
      181 tgtatagtaa aaaggagccc acgcatgagc tcctccggct acgggcccct caagcatccg
      241 cagcacaacc actaaagata gtatatgggg ttgacttcag aaagtcaagc tgtttttagt
      301 ggtagtatta gtttattcat ataagcatca aatattcgaa aatagaagca tgtgataatg
      361 aagttaatag tatcatttta ttatcaatga gaatacaaaa acccagaaaa ccgttcaaaa
      421 cgataatcca ggctttaaat agagcagatg acgggaatcg aacccgcctc ctcagcttgg
      481 gaagctgata ttctaccgat gaactacatc tgcgtgatag aatcattata tcagccccaa
      541 acggtttttg caagagaatt tttggaaaaa tttataattt tataacaatt tagaatataa
      601 tcccataagt atcagagcgc cgccggatcc gatcatgatc caggaaagag ggaaagcgtc
      661 tgccatggga ccaaagaggg ccattcccag gggaagaaag ccggaataca tggttcccaa
      721 aagaccgaat acacggccct gcatggagac gtctgtgtgt tcctggataa gcgtggtgat
      781 ggaggtttgc accacggtca aagcaatccc atagaaaaac atcatgataa gatagggaat
      841 aaaataagca gacagtccca tcccaatagc aaaggctcca aagctgaaaa gcccccatga
      901 taaaaggcgg gttttgcttt ggccgttatt tttaaggctc atcaggatcc cgcccaggat
      961 cattcctcca aagccgatca cctccacggc ggtaaggtac cagtaggtat ccccaaagct
     1021 tctgcgcaca aaaagtccgg ccaggaagcc cgcaggaacg cataggaagg taaaaagccc
     1081 atataggatc agaagccttc cgatagtacg gtgggaaaag gcatagttca ctccggcctt
     1141 catatcctga ataaatgagg aacactgtcc ctggctttta caggagggaa tggacaggca
     1201 ggataaaagg ccgattccca aaatggcagt cacaatatcg atcatcaggg ttgtatccag
     1261 ggtgctgact gcaaagatgg ctccggcggc agcaggagcg gcaaactgta ccgcagactg
     1321 catggctgca ttgattccgt tgcagcgcat caggtgctcc tctggtacca gctgaggaat
     1381 gactgcattg acagcagggg tctgtatgcc ggctccaagg gaacggatga cagacattaa
     1441 aagaagccct ccaagaaaaa gcggtttgga ggtgatgtgc gggactgcaa gcagcatggc
     1501 cagagtagcc agcgccgtaa gggcatctgc tcctgcgatc agcagttttc tgctgtaccg
     1561 gtccgcccat acgcctcctg aaaaggagat gagaaactgg ggcagatagg agcacactgt
     1621 aaatgctgca acccaggctc cgcttgccgt ttccatggtg gcgtaccaga ccacggccat
     1681 ctgtaccagg gtggaaccaa atagggtaat gcactggctg gtcagaaaca ggattgtccg
     1741 tctctgccag ccgcttggtt ttgtttcatt cattgtttgt ttcattgaat cgtatcctcc
     1801 tgtattttta gccgcggatg ttccatatta gcagactttt tctaaaaata caagttttta
     1861 tttccggatt cagccagagg tataatcagg aatagatccg gccattcttt ttataaaaga
     1921 ataatttttc agaagggaaa tgttatgtac aaaaaggata tctgtgaaaa aaagaaacag
     1981 gaaagagact gccttgttat ggatcttgat cttgtgatcg gcggccgccc ggaccgcgtc
     2041 cgggagcttt tagcacacct tgattttgat atgcatcctc cggggccgga gctgaaacgc
     2101 cttgcagaag agctggaaaa gaagcttgag gcccaatgaa cttgaaaaaa tcattcaaat
     2161 atgatttaat actatgagac aaaccggcgc gcaccttaat ttttaagggg cgtgcttcat
     2221 gaatatggag gataagaatg gcaacagaac aatataatgc ggacagtatt accgtcttag
     2281 agggcctgga ggccgtccgc aagcggccgg gtatgtacat aggtggcgtt ggtaccaagg
     2341 gactgaacca tctgatctat gagattgtag ataatgcagt ggacgagcat ctggctggct
     2401 tttgctctaa gatctgggtg aaactggaaa aggacggttc ctgcactgta aaggacaatg
     2461 gacgagggat tccggtggaa atgcaccaga aaggcgtttc ggcagccaga atcgttcttt
     2521 ccacccttca tgcgggcggc aagtttgaca atgaggcata taaaaccagc ggcggtcttc
     2581 acggcgtagg ctcctctgtg gtaaatgccc tttcggaata tatggatgta aagatcagcc
     2641 ggggcggtct ggtctatcat gatgcctatg agaagggccg tccggtggtt gagctggagc
     2701 agggccttct tccggttgtg ggaaagagca gggaaagcgg cacccagatc aattttcttc
     2761 cggatggaga aatctttgaa aagacacgct ttaaggcaga ctggctgaaa agccgtctcc
     2821 atgagacagc ctatctgaat cccaatctga cgatttttta tgagaatcac cggagtggag
     2881 aagaggaagc cattacttac catgagccgg agggaattat ttcttatgtg aaagaattaa
     2941 attcaggaaa aactcccgtt catgagccca tttattttaa aggagaggta gaccgggtag
     3001 aggttgaggc agctatccag tttgtagata ccttcgagga gaatatcctt ggcttctgta
     3061 ataatatttt cacccaggag ggcggcaccc atctggcagg ctttaaaaca cgttttactg
     3121 ctatgatcaa ttcttatgcc agagagcttg gtattttaaa ggaaaaagat cctaacttta
     3181 cgggagcgga cacccgaaac ggtatgacag ctgtgatcgc agtgaagcat ccggacccta
     3241 tctttgaggg acagaccaag acaaagctgg caagcgcaga cgctaccaag gcagtattta
     3301 ccgtagcggg agatgagctg cagctgtact ttgaccggaa tgtggaggtc ttaaagggag
     3361 tgatcggctg tgcggaaaag tctgcaaaga tccgcagggc tgaggaaaag gcaaagacaa
     3421 atatgctgac caaatcccgt ttttcttttg acagcaacgg aaagcttgca aactgtgaga
     3481 gccgggacgc ttccaaatgt gaaattttta tcgtagaggg agattctgcc ggcggctctg
     3541 caaagacagc cagaaaccgg cagtatcagg ccattctgcc tatccgcgga aagattttaa
     3601 atgtagaaaa ggcttctatg gataaggtgc tggcgaatgc ggagatcaag accatgatta
     3661 attcctttgg ctgcggcttt tcagagggtt atggaaatga ttttgatatc acgaagctgc
     3721 gctatgacaa gattatcctc atgacggatg cggatgtgga cggaagccat attgatacgc
     3781 tgcttcttac ctttttatac cggtttatgc cggagctgat ctatgacggc catgtgtata
     3841 ttgccatgcc gcctctgttt aaggtaatcc ccaaaaaggg ggaggagcag tatctgtatg
     3901 atgaaaagga gctggaacgt tatcgcagga cccacaaggg tgattttacc ctgcagcgct
     3961 ataagggtct tggtgaaatg gatgcggagc agctctggga aacgaccctg gatccggaac
     4021 gccgggtatt aaagcgggtt gagatcgagg acgcaagact ggcttctgag gtgacggaga
     4081 tgctgatggg cagcgaggta ggcccccgcc gccagtttat ctatgagcac gccgatgagg
     4141 ctgagatcga cgcataacag gagaggacaa aatggcagaa aagatattac ggacagaata
     4201 cagcgaggaa atgcagcgca gctacatgaa ttattccatg agcgtcatta ctgcaagagc
     4261 gattcccgac gccagggacg gcttaaagcc cgttcagcgc cgggtgctct atgatatgag
     4321 ccagctgcgc ctggattacg acaagcccca caggaaatcc gcccgtatcg taggcgatac
     4381 catgggtaaa taccatcccc acggcgacag ttctatctat gatacgctgg tagttatgag
     4441 ccaggaattt aaaaagggaa tggctctggt agacggccat ggcaatttcg gctctattga
     4501 gggtgatgga gcggcggcta tgcgatacac ggaggcaagg ctggagaaat ttgcccagga
     4561 ggtctattta aaggatttgg ataagaccgt aaatttcgtt cccaattatg atgaaacgga
     4621 gaaggagccg gaggttcttc ccgtccgggt gccgaacctt ctggtaaacg gagctgaggg
     4681 aattgcggtg ggcatgagca ccagtatccc ccctcacaat ctgggagagg taattgatgc
     4741 ggtaactgcc tatattgaca atcccgcaat cactgcggca gagctgatgg agcatatgcc
     4801 ggggccggat ttccccaccg gaggcattat cgcaaataag agcgaccttc cggagatcta
     4861 tgaaagcgga acgggaaaaa taaagctcag aggccgtttt gaggtggagc ttggaaagcg
     4921 caaggcagat aaggacaagc tggtgatcac ggaaattccc tataccatga tcggagcagg
     4981 tatcaataag tttctggtgg acgtagccga tctggtagag tctaaaaagc ttacggatgt
     5041 ggtggatatt tccaatcagt ccaataagga cgggatccgt atcgtactgg agctgagaaa
     5101 ggacgcggat attgaccgga tcaagaacat tttatataag aagacaaagc ttgaggatac
     5161 ctttggcatc aatatgctgg ctattgcgga cggacgtccg gaaacgctga atttaaaggg
     5221 gattctccgc aattttctgg aattccagta tcagaatacg gagcgtaaat acaatgtgct
     5281 tctggaaaag gaactggaaa agaaggaaat tcaggagggt ctcataagcg cctgtgactg
     5341 cattgacctg attatcgcaa ttttaagagg ttctaaaagc ttaaaggatg cgaaggcctg
     5401 tctgatatct ggtgatgtga gcaatatcca tttccggatg ccgggatttg aggaggaggc
     5461 aaagaagctc cactttacag agcgccaggc gtctgctatt ttggaaatgc ggctgtacaa
     5521 gctgatcggt ctggaaattc tggctctgga aaaggagcat aaggaaacct taaaacgaat
     5581 tgaaagctac aaaaagatcc tgggcagccg tggggtgatg aaccgagtga tcaaggacga
     5641 tctggcagct atccggaagg aatttgcagt cccaagacgg acgcggatcg aggacggccc
     5701 ggaggcagtt tatgtggaaa atgcagttcc ggttcaggag gttgtttttg tgatggatcg
     5761 tttcggctat tgtaagcttt tggataagtc tacttacgaa cggaatcagg aaacggtgga
//
LOCUS       DBMDUH010000164         5792 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_5488_length_5792_cov_8.876999, whole genome shotgun sequence.
ACCESSION   DBMDUH010000164 DBMDUH010000000
VERSION     DBMDUH010000164.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 5792)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5792)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5792
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5488_length_5792_cov_8.876999"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            61..1140
                     /locus_tag="ACZPCG_11485"
     CDS             61..1140
                     /locus_tag="ACZPCG_11485"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR1285706.1"
                     /translation="MRRIGILTSGGDCQALNATMRGVAKSLYNMLDDVEIIGFEDGYK
                     GLMYADYHIMKPSDFSGILTQGGTILGTSRQPFKQMRVPDENGLDKVESMKHTYRKLK
                     LSCLVVLGGNGSQKTANLLSEEGLNVVSLPKTIDNDLWGTDMTFGFHSAVEIATNAID
                     CIHTTAASHGRVFIVEVMGHKVGWLTLYAGISGGADIILLPEIPYDINVVCDAITKRS
                     KAGKRFSILAVAEGAISKEDAGMTKKELKEKKKNGVVYPSVAYEIGARIQEVTGSEVR
                     VTVPGHMQRGGEPCPYDRVLATRLGAAAARLIADEEYGYMVALKNNEITKVPLSEVAG
                     KLKTVDPGCSMIKEAKMVGISFGDE"
     gene            1153..2844
                     /gene="dnaX"
                     /locus_tag="ACZPCG_11490"
     CDS             1153..2844
                     /gene="dnaX"
                     /locus_tag="ACZPCG_11490"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009268759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HIR1285707.1"
                     /translation="MSYMALYRKWRPVSFEDVKGQDPVVQTLKNQITSDRIGHAYLFC
                     GTRGTGKTSIAKIYARAVNCEHPIDGSPCNQCASCRAILEGSSMNVVEIDAASNNGVE
                     NIRDIREQVQYPPTEGKYRVYIIDEVHMLSIGAFNALLKTLEEPPAYVIFILATTEVH
                     KIPITILSRCQRYDFKRISLETIADRLRELTQAEQIQAEDKALMYIAKAADGSLRDGL
                     SLLDQCVAFHYGKLLTYENALEVLGAVDSSVFSRMFNAVADGRTRDCITELEEIVIQG
                     RELGQFVTDFIWYLRNLLLVQSADDPEGLVDLSEENLKQLKADAVKTDGNTLMRYIRV
                     FSDLSSQLRYASQKRVLLEVALIRLTHPAMEPDLDSVLQRIHQLEEQLEDIEARGISA
                     GQGTYGAAGSMAAFGTCGLPGQPSAGSDPAGSLQQSPSGDSGQTSAGISMGSASETIS
                     LPKAQLEDLNLIRNEWAKIVRSIGGGARSYLRDTVVEPGGEGCLTIVFLDPMSYDMGK
                     RPTVIGELERKVEAAYGKSIYFKTRLAGRGERLDTIYVTKEDLEEKIHMDITYED"
     gene            2903..3259
                     /locus_tag="ACZPCG_11495"
     CDS             2903..3259
                     /locus_tag="ACZPCG_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HIR1285708.1"
                     /translation="MAKRGGFPGGMPGNMNNLMKQAQRMQRQMEETTKALEEKEYTAA
                     AGGGAVTVTVSGKKEVVSIKLAEEVVDPDDIEMLQDLIVAATNEAFRQMEADSAEAMS
                     RLTGGLGGALGGGFPF"
     gene            3259..3855
                     /gene="recR"
                     /locus_tag="ACZPCG_11500"
     CDS             3259..3855
                     /gene="recR"
                     /locus_tag="ACZPCG_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007707929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HIR1285709.1"
                     /translation="MDYYSSQISRLIEELSKLPGVGAKSAQRLAFHIINMPKEQVEQL
                     AGAMTGARNNVRYCKECFTLTDKELCPICSSTKRDHSTIMVVENTRDLVAYEKTGKYN
                     GVYHVLHGAISPMLGIGPNDIKLKELMQRLQLDVEEVIIATNSSLEGETTAMYISKLI
                     KPTGIRVTRIASGVPVGGDLEYIDEVTLLRALEGRTEL"
     gene            3886..>5792
                     /locus_tag="ACZPCG_11505"
     CDS             3886..>5792
                     /locus_tag="ACZPCG_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285710.1"
                     /translation="MDKYEFNLRIDQIKKKIASEDFETAMKIADTIDWRRVRNINILS
                     MVSGVYEKNGEYQEAKDILLLAFERAPMGKRLLYKLAELAIKEGSPDEAEDYYREFCE
                     LAPEDPRQYILRYLILGAKGAPVEQLIHTLEQYCNVELDEKWLYELAELYNAAHMEQL
                     CVMTCDKIMLMFGTGKYVDKAIDLKVQYAPLTDYQMDLVENKDKYEARLKAVETEYNK
                     KKPMVLPIDEEDAQAEQEAEDAYVYQQEAAYTKEPEPEEKVLEEQPETVFDDEETGEE
                     EAFSNAVQEPEEEQELSCEEEESEADSEPEVSSEPEVVSGSSKEDETLRAQLHEAEAQ
                     EDLAKEMSRLAEEHGYSEETQAAQTQVLADIRHLKKGPMYSGSNHLMIEADEPEKGFE
                     LAVKTLKRIHQETGAKNQAAKITGARLNNRGIFTLSDKLTGKDLIIEQAGDMNESILQ
                     ELNQLMARDETGMSVVLIDTAERLEELHKTYPGLAKRFECIGTSVKEQEKEAEPDNRP
                     VRAVRLHEAPASAAPVRMKKMVEQEAIETKGNVLPSRPAYQAAFHPSETEEEKRPAAP
                     SEKAGETREAVQAAKPAAAKRRQVIPLVPEPPVQTEKEPVLEEPEQEMDIDEFAKYAC
                     EYAGSIDCSISG"
BASE COUNT         1659 a         1175 c         1643 g         1315 t
ORIGIN      
        1 gtatcagtgc ccacacggcc ggtatgcaga gtctgacact ctgaaatgga ggaacgcaat
       61 atgagaagaa taggaatctt aaccagcggt ggcgactgcc aggcattaaa cgctactatg
      121 cgcggtgtgg caaaatcctt atataatatg ctggatgatg tagagatcat tggttttgag
      181 gacggttata aggggctgat gtatgcagat taccatatta tgaagccatc cgatttttcc
      241 ggtattctca ctcagggcgg aacgatcctg ggaacttccc gccagccttt taagcagatg
      301 cgtgtgccgg atgagaatgg tctggataag gtagaatcta tgaagcatac ctatagaaag
      361 ctgaaactca gctgtctggt cgttctcggc ggaaacggaa gccagaaaac agccaatctc
      421 ctcagcgagg agggccttaa cgtggtttcc ctgcccaaaa ccattgataa tgatctctgg
      481 ggaacggata tgaccttcgg atttcacagt gcagtggaga ttgcgaccaa tgccattgac
      541 tgtatccata ccacagccgc ttcccatggc cgtgtattta ttgtagaggt tatggggcat
      601 aaggtaggct ggcttaccct ttatgcaggc atttctggcg gagccgacat tatccttctt
      661 cctgaaatcc catatgatat caacgtagtc tgcgatgcga tcacaaagcg ttccaaagca
      721 ggcaagcgtt tttctattct ggcagtggca gagggggcta tttcaaaaga agatgccggc
      781 atgacgaaaa aggaattaaa ggaaaagaaa aagaacggag tggtttatcc gtcggttgcc
      841 tatgagattg gagcaagaat ccaggaggtg acaggaagcg aggtccgcgt cactgttcca
      901 ggacatatgc agcgaggagg agaaccttgt ccttacgacc gtgtgcttgc taccagactg
      961 ggtgcggctg cagccagact gattgcagat gaggaatatg gctatatggt ggctttaaaa
     1021 aataatgaga ttaccaaggt gcctctctct gaggtggcag gtaaattaaa gaccgtggat
     1081 cctggatgct ccatgataaa ggaggcaaaa atggtcggga tcagtttcgg agacgaataa
     1141 ggagcaggcc ctatgtcata tatggcttta taccggaaat ggcggccggt gtcctttgag
     1201 gatgttaagg gccaggatcc ggtagtacag acactaaaaa atcagattac ttcagatcgg
     1261 atcggtcatg cctatctgtt ctgcggaacc agaggtacgg gaaaaaccag tattgcaaaa
     1321 atctatgcca gagcagtaaa ctgtgagcat ccgatcgatg gaagtccatg caaccagtgt
     1381 gcttcctgcc gcgccatttt agagggaagc tctatgaatg tggtggagat tgatgcggct
     1441 tctaacaatg gcgtggaaaa tatccgggat atcagggaac aggtacagta tccgccgaca
     1501 gagggaaaat acagagttta catcattgat gaggttcaca tgctttccat tggagccttc
     1561 aatgctttgt taaaaacatt ggaggagccg ccggcttatg tgatttttat tctggcgact
     1621 acagaggttc ataagattcc tattacgatt ttatcccgat gccagagata tgattttaag
     1681 cgcatttctc tggaaaccat tgcagaccgg ttaagagagc tgactcaggc agagcagatc
     1741 caggcagaag ataaggcgct aatgtatatt gcaaaggctg ctgatggctc tttgcgagac
     1801 ggcttaagcc tgcttgatca gtgtgttgcc ttccattacg gcaaacttct gacttatgaa
     1861 aatgcccttg aggttctggg ggcagtggat tccagcgtat tcagccgtat gttcaatgcg
     1921 gttgcagatg ggagaacccg cgactgcatc acagaactgg aagaaattgt aattcagggc
     1981 cgggagctgg gacagtttgt tacagatttc atctggtatc tgcgaaatct tcttctggtt
     2041 cagagtgcag atgatccgga ggggttagtg gatctgtcgg aagagaatct aaagcagctg
     2101 aaggcagatg ctgttaagac agacggaaat acccttatgc gctatatacg ggtattttca
     2161 gatttgtcca gccagctgcg ctatgccagc cagaaacgtg ttttgctgga agtcgctttg
     2221 atccgtctga cacacccggc tatggagccg gatctggatt ctgttttaca gaggattcat
     2281 cagctggagg aacagttaga ggatatagag gccagaggaa tcagcgcagg ccaggggact
     2341 tatggagcgg caggcagtat ggctgccttt ggaacttgtg gccttccggg gcagccgtca
     2401 gccggctccg atcctgcagg cagtttgcag cagtcccctt ccggtgattc gggacagacg
     2461 tctgccggaa tttcaatggg ttcagcgtca gaaaccattt cccttcccaa agcgcagctg
     2521 gaggatttaa acctcatacg caatgaatgg gccaaaattg tccgcagcat cggcggcgga
     2581 gccagatcct atctgcggga taccgtagta gaacccggag gggaaggctg tcttaccatt
     2641 gtatttttag accctatgag ctatgatatg ggcaagcgcc ctacggtgat tggagaattg
     2701 gagcggaaag tagaggctgc ctacggcaaa tccatttatt ttaaaacaag actggcaggc
     2761 cggggtgaac gtctggatac tatttacgtt acaaaagagg atctggaaga aaagatccat
     2821 atggatatta cttacgaaga ctgatatatt ttcatataaa acaggcctgt aaaggccaga
     2881 cagaaagagg aggaacatca ccatggcaaa acgtggcgga ttcccgggcg gtatgccggg
     2941 aaatatgaat aatcttatga agcaggcaca gcgtatgcag cgccagatgg aagagacaac
     3001 aaaggcatta gaggaaaagg aatatacagc agcagcaggc ggcggagcgg taacagtaac
     3061 ggtttccggc aaaaaggaag tagtttctat taagctggca gaagaggtag tggatccgga
     3121 tgatattgag atgcttcagg atctgatcgt ggcagctacc aatgaggcct tccgccagat
     3181 ggaggccgac agtgcagagg ccatgtccag actgaccgga ggactgggcg gtgctttagg
     3241 cggaggcttt cctttctaat ggattattac agcagtcaga tcagcagact gatcgaagaa
     3301 ttgtccaagc tccccggagt aggcgcaaaa tctgctcaaa gacttgcgtt tcatatcata
     3361 aatatgccta aggagcaggt agagcagctg gcaggagcca tgaccggcgc cagaaataat
     3421 gtccgctact gcaaagagtg ctttacgctc acagacaagg agctttgtcc aatctgcagc
     3481 agtacaaaac gcgaccacag tacgattatg gtagtagaaa acacccgcga cctggtggct
     3541 tatgagaaaa ccggcaaata taacggagtc tatcatgtgc tccatggagc gatttctccc
     3601 atgctgggga ttggccctaa tgatattaag ctgaaggagt taatgcagcg ccttcagttg
     3661 gatgtggagg aagttattat tgcaaccaat tccagtctgg aaggcgaaac gacagcaatg
     3721 tatatcagca agctgatcaa gccgaccggg atcagagtta cacggatcgc cagcggtgtg
     3781 cccgtaggcg gagatctgga atacatcgac gaggtaacgc tgcttcgggc gctggaagga
     3841 aggaccgagc tttaaaatgc cgcttacggc agggagatgc ataagatgga taaatatgag
     3901 tttaatttaa ggattgatca gattaagaag aaaattgcct cagaagattt tgagacagcg
     3961 atgaagatcg cggataccat tgactggaga agagtccgca atatcaatat cctgtctatg
     4021 gtttccgggg tttatgaaaa aaatggagaa tatcaggaag caaaggatat tctccttctg
     4081 gcttttgaac gggcccctat gggaaagcgc cttctgtaca agctggccga gctggcaatt
     4141 aaagaaggaa gtccggatga ggcagaggat tattaccggg aattttgtga gctggctccg
     4201 gaagacccca gacagtacat tctgcgctat ttgattttgg gagctaaggg agcgccggta
     4261 gagcagctga tccatactct ggagcagtac tgcaatgtag aactggatga gaaatggctt
     4321 tatgagctgg cagagcttta taatgcggcc catatggagc agctttgtgt gatgacctgt
     4381 gacaagatta tgcttatgtt tgggacagga aaatatgtag acaaggcgat tgacttaaag
     4441 gttcagtatg ctccccttac agactatcag atggatctgg tggaaaataa ggataaatat
     4501 gaggcccgat tgaaggctgt agaaacagaa tataataaga aaaagcctat ggttcttccg
     4561 attgatgagg aagatgcaca ggcagagcag gaagcagagg atgcttatgt gtatcagcag
     4621 gaagcagcct atacaaaaga gcctgaaccg gaagagaaag ttttagagga gcagccagag
     4681 acggtgtttg acgacgaaga aacaggggaa gaagaagcct tttccaatgc tgtacaggag
     4741 cctgaagagg aacaggagct gtcctgtgag gaagaagaat cagaagcgga cagtgagccg
     4801 gaggtgtctt ctgagccgga agttgtttcc ggctcatcaa aagaagacga aactctccgt
     4861 gcacagcttc acgaggccga agctcaggag gatcttgcga aggaaatgtc ccggctggca
     4921 gaggagcatg gatacagcga ggaaactcag gcggcccaga cacaggtgct ggcggatata
     4981 cgtcatttga aaaaaggacc gatgtatagc ggatcaaatc atctgatgat tgaggcagat
     5041 gaaccggaaa agggttttga gctggcggta aagacattaa aacggattca tcaggaaaca
     5101 ggcgctaaaa atcaggctgc aaagattaca ggagcccgtt taaataacag aggaattttt
     5161 actctgtcag acaagcttac gggaaaagac ctgattatcg aacaggccgg agatatgaat
     5221 gagtcgattc ttcaggagct caatcagctt atggcaagag atgaaaccgg tatgagtgtg
     5281 gtgctgatcg ataccgcaga gcgtttggag gagctccaca aaacctatcc gggactggct
     5341 aagaggtttg agtgcattgg gacctctgtg aaggagcagg agaaagaagc agagccggat
     5401 aaccgtccgg ttcgtgcggt gaggcttcat gaagctcctg catctgcggc acctgtccgc
     5461 atgaaaaaga tggttgagca ggaagcgata gagacgaagg gaaatgttct tccgtcccgt
     5521 cctgcgtatc aggcagcttt tcacccttct gaaacggaag aagagaagag gccggcagca
     5581 ccatccgaaa aagctggaga aacaagagag gctgtacagg cagctaagcc ggctgcggcg
     5641 aaacgcagac aggtaattcc tctggttccc gaacctccgg tacagactga gaaagagcct
     5701 gttttggaag aacctgagca ggaaatggat attgatgagt ttgcaaaata tgcctgtgaa
     5761 tatgccggca gtattgactg cagtatttca gg
//
LOCUS       DBMDUH010000165         5779 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_5503_length_5779_cov_6.001585, whole genome shotgun sequence.
ACCESSION   DBMDUH010000165 DBMDUH010000000
VERSION     DBMDUH010000165.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 5779)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5779)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5779
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5503_length_5779_cov_6.001585"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="ACZPCG_11510"
     CDS             complement(<1..476)
                     /locus_tag="ACZPCG_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HIR1285711.1"
                     /translation="MVRVKIGTDIREYPQGTTWAQVAEEYQSGFEHDILLVQIGGKLQ
                     ELHKTVRDGSLRFITAEEKPGMSAYQRSATLLMLKALYAVAGAERVEKVIVDFSVGKG
                     FFVEARGAFTVDDGFLEQVKAKMEEYVNRKIPIQKRSVSTDDAIELFHDHHMYDKER"
     gene            complement(533..1342)
                     /locus_tag="ACZPCG_11515"
     CDS             complement(533..1342)
                     /locus_tag="ACZPCG_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HIR1285712.1"
                     /translation="MKCSDIIKILQETAPESCACDWDNPGLLAGRSDKEVKKIYIALD
                     ATDEVVDAAIREKADLLLTHHPLIFRPVKKVNDQNFITRRLVRLIQADISLYAMHTNF
                     DAAPEGMAALAAERLDIREIRPLECMGEMKENGTSVPYGIGAVGSLYEPMTVNGLAEK
                     VKKAFDLPFVLVYGPGLMDKEVKRAALCPGSGSSVIEQAVLAGADVLITGDIGHHPGI
                     DAAARGLAVIDGGHYGLEHIFIQHMAEVLREKLKGEAALVLAEPAWPSVLL"
     gene            complement(1329..2063)
                     /locus_tag="ACZPCG_11520"
     CDS             complement(1329..2063)
                     /locus_tag="ACZPCG_11520"
                     /EC_number="2.1.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007717152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0160105 - tRNA
                     (adenine(22)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase"
                     /protein_id="HIR1285713.1"
                     /translation="MTNHTANFVPRLSERLKLVASFVPQDSRIADIGTDHGFVPICLA
                     KEGRIKSALAMDVRTGPLERAEAHIKEAGISDIPISTRLSDGLKELKPQEADTVIIAG
                     MGGELEIKILQEGRHLWSTIRRFILSPQSDLEKVRRFLSEEGFLIEKEAMLREEGKYY
                     TVMSVVRGAMVYNRTIEYRYGKYLLEQKEPVLKEYLKKEEERVLAILASLSAKEKGEL
                     TKGQEKARQSLLEELSQIREAEYEVQ"
     gene            complement(2099..3955)
                     /gene="dnaG"
                     /locus_tag="ACZPCG_11525"
     CDS             complement(2099..3955)
                     /gene="dnaG"
                     /locus_tag="ACZPCG_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HIR1285714.1"
                     /translation="MYYPDEVIEEVRMKNDIVDVISGYVKLQKKGANYFGLCPFHNEK
                     SPSFSVSPGKQMYYCFGCGAGGNVLTFVMEYENYTFQEALTALADRAGVSLPKMEYSK
                     EAREQAEFRSRLLEVNKLAANYFYYQLKQPQGKAGYEYFKEKRGLTDETILRFGLGYS
                     NKTSDDLYRFLREKGYEDSFLKETGLVTVEERGGRDKFWNRVMFPIMDVNNRVIGFGG
                     RVMGEGEPKYLNSPETRLFDKSRSLYGLNYARTTREKFMLICEGYLDVISLHQAGFTN
                     VVASLGTAFTSQHAGILKRYTDQVILTYDSDGAGVKAALRAIPILREAGLSARILNMS
                     PCKDPDEFIKQMGPDAFRKRILEAKNSFLFEIDVLKRQYALDDPEQKTKFYQETARKL
                     LQFGEPLERDNYLQAVAREHMIKEDELRQLVNRMGMQLGLKAGDSYREDAGGRGYRET
                     APDEGASFKGASEGRTGNGDGTKSRRRPEREDGIRRSQRLLLTWLIEDPALFDKIRGV
                     ITADDFVEELYHQVAVMVFEGHEQGSLNPAAILSRFINDDDQYREVAALFNASLKESL
                     SNEEQKKAFSETVLKVRRHSLDTASRNAKDIAQLQEIIKQQAALKQISISLD"
     gene            complement(3980..4987)
                     /locus_tag="ACZPCG_11530"
     CDS             complement(3980..4987)
                     /locus_tag="ACZPCG_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HIR1285715.1"
                     /translation="MNVRESLEALEEQTLSPYASFSSRTRGRDHAEPLCDIRPEYQRD
                     RDRILHCKAFRRLKHKTQVFLAPEGDHYRTRLTHTLEVSQIARTIAKSLRLNESLTEA
                     IALGHDLGHTPFGHSGEAVLDQICEDGFTHYEQSVRIVEVLEKDGKGLNLTWEVRDGI
                     LNHRTAGHPSTLEGAIVRLSDKIAYINHDIDDAIRARMFAEEELPKEYTDVLGNSVRE
                     RLNRMIRDLILNSMDQPQIIMSSDMEEAMKGLRRWMFEHVYRNDIPKAEESKAQEMIA
                     RLYEYYMKHADRLPMEYILLMVNGEKKSRVVCDYIAGMSDIYAIDRFEALFVPKRWNV
                     Y"
     gene            complement(5109..>5779)
                     /locus_tag="ACZPCG_11535"
     CDS             complement(5109..>5779)
                     /locus_tag="ACZPCG_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009263203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR1285716.1"
                     /translation="VPYADQGLNKIPDEVSDEQALFVGDVLATGFWAARISEITEEDT
                     VLIIGGGPTGICTLLCVMLKKPEKILLCEQDEDRRRFIKEHYPEVIILSPEQIREAVK
                     RESRHGGADVVLEVAGTEDTFRMAWECARPNGVVTIVALYDKPQVLPLPEMYGKNLTF
                     KTGGVDGCDCEEILSLIREGKIDTTPLITHRFALSEIDAAYDVFENRKDHVIKAAVVP
                     DRQM"
BASE COUNT         1335 a         1670 c         1185 g         1589 t
ORIGIN      
        1 cgctctttat catacatatg atgatcgtga aacagctcga tcgcatcatc cgtggataca
       61 ctgcgctttt ggatgggaat tttccgattt acatattctt ccatttttgc ctttacctgc
      121 tccaaaaaac catcgtccac agtaaatgcg cctcttgcct ctacgaaaaa accctttcct
      181 acggaaaaat ccacgatcac cttttccacc cgttctgccc cggccactgc atagagggcc
      241 tttagcatca gcagggtagc gctgcgctga taggcagaca ttcccggctt ttcctctgca
      301 gtgataaagc gcagactgcc gtccctgacg gtcttgtgaa gttcctggag ctttccacca
      361 atctgtacca gcaaaatatc atgttcaaaa ccactctgat actcttctgc cacctgcgcc
      421 caggtcgttc cctgaggata ttctcttatg tctgttccaa ttttcacccg taccatatgc
      481 ttacctcccc acttctgatt ttctgctgcc gctataagca gctgccgctt tcctataaaa
      541 gaacggacgg ccaggccggc tctgccagaa caagcgctgc ctcacctttt agtttttccc
      601 gcagaacctc agccatatgc tggataaaaa tatgctccag cccgtaatgg cctccgtcaa
      661 tgacggcaag tcccctggct gccgcgtcaa ttcccggatg atggccgata tcaccggtga
      721 tcaaaacatc ggctcctgcc aaaaccgcct gctcaataac gcttgatccg gagccgggac
      781 agagagctgc ccttttcact tccttgtcca tcagtcccgg cccgtaaacc agcacaaaag
      841 gaagatcaaa agctttcttt accttctccg ccagaccgtt caccgtcata ggctcataaa
      901 ggctgcccac agccccgatt ccgtagggga cggatgttcc attttctttc atctccccca
      961 tacactctaa aggacgtatt tcccttatat ccagccgctc tgctgccaga gctgccattc
     1021 cctcaggcgc ggcgtcaaaa tttgtatgca tggcatacag ggaaatatct gcctggatca
     1081 gccgcaccag ccgccttgta ataaaattct ggtcatttac cttcttaaca ggacggaaaa
     1141 taaggggatg atgggtaaga agaagatccg ccttttcacg aatggctgca tctacaacct
     1201 catccgtggc atccagggca atgtatatct tttttacctc cttatcgctt cttcccgcta
     1261 acagaccggg attatcccaa tcgcaggcgc agctttccgg agccgtttcc tgtaatatct
     1321 ttatgatgtc actgcacttc atattctgcc tccctgatct gtgataattc ttctaaaagg
     1381 gactgtctgg ctttttcctg cccttttgta agctcccctt tctcttttgc ggaaagcgat
     1441 gcaaggattg ccaaaacccg ttcctcctct ttttttaaat attcttttaa aacgggttcc
     1501 ttctgttcaa gaaggtattt tccatagcgg tattcaatgg tcctgttata aaccatagct
     1561 ccccgcacca cgctcataac cgtatagtat tttccttcct cccggagcat ggcctctttt
     1621 tcaatgagaa acccctcttc tgaaaggaag cggcgtacct tttcaagatc tgactgaggc
     1681 gaaaggataa aacgccggat cgtgctccat agatgccttc cctcctgcag aatctttatc
     1741 tccaattcgc ctcccattcc ggcgatgatc accgtgtctg cctcttgggg ctttaactct
     1801 tttaatccgt cgcttaaacg ggtgcttata gggatatctg aaattcccgc ctcctttatg
     1861 tgagcctccg cccgttccag agggcctgtc cgcacatcca tggcaagggc gctttttatg
     1921 cggccttctt ttgccagaca gatgggaaca aagccgtggt ccgtaccgat gtctgcgatc
     1981 cggctgtcct ggggcacaaa ggaagccacc agcttcaggc gctcagacag acggggaaca
     2041 aaatttgctg tatgattcgt catattcatt ttcctgttcg tgtaataatt tttatgcgct
     2101 aatccagaga aatgctgatc tgctttaagg cagcctgctg ctttatgatc tcctgcagct
     2161 gggcgatatc ctttgcattg cggctggccg tgtcaagact gtgtctgcgc acctttaaaa
     2221 ccgtttctga aaaagctttt ttctgctcct cattgcttaa agactccttc aagctggcgt
     2281 taaaaagagc cgccacctcc cggtactgat cgtcatcatt gataaaacgg ctgaggatcg
     2341 cagccggatt taaactgccc tgctcatggc cttcaaagac catcaccgcc acctgatgat
     2401 acagctcttc cacaaaatca tctgcggtta taactccgcg gatcttatca aacagagccg
     2461 gatcctcgat cagccaggtt aaaagcagac gctgggagcg gcgtataccg tcttctcttt
     2521 ccggacggcg gcgggatttc gttccatccc cgttccctgt ccggccttca gatgcacctt
     2581 taaaggaagc cccttcatcc ggtgcagttt ctctgtagcc acgtcctccc gcgtcctctc
     2641 ggtagctgtc acctgccttt aatccaagct gcatacccat gcggtttacc agctggcgca
     2701 gctcatcctc tttaatcata tgctctctgg ctactgcctg aagataattg tcccgctcta
     2761 agggttcccc gaactggagc agctttctgg ctgtttcctg ataaaacttc gttttctgct
     2821 ccggatcatc cagcgcatac tgccgtttca gcacatcaat ctcaaacaga aagctgtttt
     2881 ttgcctccaa aatccgcttt ctgaacgcat ccggccccat ctgtttgata aattcatctg
     2941 gatccttaca ggggctcata tttaaaattc tggctgaaag ccctgcttcc ctgagaatag
     3001 gaatggctct taaggctgct tttacaccgg ctccatcgct gtcataggtt aagatcacct
     3061 gatccgtata ccgcttcaaa atccccgcat gctgactggt aaaggcagtc ccaagagagg
     3121 ccactacatt ggtaaaaccg gcctgatgca aagagatcac atccagataa ccctcgcaga
     3181 tcagcataaa cttctccctg gtggttctgg cataatttaa gccgtaaagg ctgcggcttt
     3241 tatcaaagag cctggtttcc ggagagttta gatactttgg ctccccttct cccatcacac
     3301 ggcctccaaa gccgatcaca cggttattta catccataat gggaaacatg acacggttcc
     3361 agaacttgtc cctgccgccg cgttcttcta cggtcacaag gcctgtttcc tttaaaaagc
     3421 tgtcctcata gcccttttcc cttaaaaacc ggtacaggtc atcactggtc ttattggaat
     3481 agcccaaacc aaagcgcagg atggtctcat ccgtaagccc gcgcttttcc ttaaaatact
     3541 catatccggc ctttccctgg ggctgcttca actgataata aaaataattg gcagccagct
     3601 tgtttacctc aaggagcctg gagcgaaact ctgcctgttc tctggcctct ttgctgtact
     3661 ccatcttggg gagactgacc ccggcccggt ctgccagagc ggtaagcgcc tcctgaaaag
     3721 tatagttttc gtattccatc acaaaggtaa gcacatttcc gcccgccccg cagccaaagc
     3781 agtagtacat ctgcttgccc ggagaaacgg aaaaggaagg cgatttttca ttgtgaaacg
     3841 ggcacaggcc gaaatagtta gctccttttt tctgcagttt tacatatcct gagatcacat
     3901 ccacgatgtc atttttcatt ctcacttctt ctatgacttc atccggatag tacatgcatc
     3961 ttctcccttc cgacttgttt tagtatacat tccaccgttt aggcacaaac agcgcctcaa
     4021 accggtcaat ggcataaatg tcactcattc ccgcaatata gtcgcagacc acccgggact
     4081 ttttctcccc atttaccatt aaaagaatat actccatggg aagacggtcc gcatgcttca
     4141 tataatattc atacagtctg gcgatcatct cctgggcctt gctttcttct gcctttggaa
     4201 tgtcatttct atatacatgt tcaaacatcc accggcgcag gcccttcatc gcctcctcca
     4261 tatctgaaga catgataatc tgaggctgat ccatactgtt taaaataaga tcccgtatca
     4321 tacggttcaa ccgttcccgc acactgtttc caagaacatc cgtatactcc tttgggagct
     4381 cctcttctgc aaacatacgg gcacggatcg catcatcaat atcgtgattg atataggcaa
     4441 tcttatccga cagccggaca atggcaccct ccagcgtgga tggatggcct gcggtccggt
     4501 gatttaaaat cccgtcccgg acttcccagg ttaaattaag accctttcca tctttttcca
     4561 gaacctctac gatgcggacg ctctgttcgt aatgggtaaa gccatcctcg cagatctgat
     4621 ctaaaacagc ttctcccgaa tgtccaaagg gcgtatggcc cagatcgtgt cctaaggcaa
     4681 tggcctctgt gaggctttca ttcagacgca gagacttggc aatggttctg gcaatctggg
     4741 atacctccag agtatgcgtc agccgggtgc ggtaatgatc tccctccggc gccaaaaaca
     4801 cctgggtttt atgcttcagc ctgcggaatg ccttacagtg gaggatccga tccctgtctc
     4861 tctgatactc cggccgtata tcacagagcg gttctgcatg atcccttccc ctcgtccggg
     4921 aagaaaagga agcatatgga cttaaggtct gttcctccag ggcctctaat gactctctaa
     4981 cattcattcc gcacctcttt tctcttatat ctgcctgatc tgtaaacaaa agcaccggct
     5041 gcgtcttttt gcagtcgatg ctcttattat atcacaaatg tatttgaatg aacagcgcgg
     5101 cttttcccct acatctgcct gtccggcact accgctgcct tgatcacatg atctttgcgg
     5161 ttttcaaaca catcataggc agcgtcaatc tctgacagag caaaccggtg tgtaattaat
     5221 ggtgtggtat caatcttccc ctcccggatc agcgaaagaa tttcctcaca gtcacagcca
     5281 tctacgccgc cggttttaaa ggtcagattt ttcccataca tttcaggaag aggcaatacc
     5341 tgaggcttgt catagagagc cactattgtt acaactccat tggggcgggc acactcccag
     5401 gccatgcgga aggtatcctc tgtccctgct acctccagaa ccacatcggc ccctccatgg
     5461 cggctttctc tctttaccgc ttcccggatc tgctctggag aaaggataat tacctctgga
     5521 taatgctcct ttataaaacg gcgtctgtct tcatcctgtt cacagagcag aatcttttcc
     5581 ggcttcttta acatcacgca cagcagggta cagatgcctg tggggccgcc gccaatgatc
     5641 agcacggtgt cttcttctgt aatttctgat attctggctg cccagaagcc ggtcgccagc
     5701 acatccccca caaaaagagc ctgctcatca ctgacctcat ccggaatttt atttaatccc
     5761 tgatccgcat agggaacac
//
LOCUS       DBMDUH010000166         5718 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_5574_length_5718_cov_6.533559, whole genome shotgun sequence.
ACCESSION   DBMDUH010000166 DBMDUH010000000
VERSION     DBMDUH010000166.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 5718)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5718)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5718
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5574_length_5718_cov_6.533559"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            294..1010
                     /locus_tag="ACZPCG_11540"
     CDS             294..1010
                     /locus_tag="ACZPCG_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285717.1"
                     /translation="MGTQYIRWFDEYAGSFFKVSARDERPYICGERCWQLRCEYLHQN
                     KGFLNDENEDTVHFHLGVNCGTSLCELDRTRGQADRSDIRIDIEQFCLRMYQAAKSYY
                     DQVHKEKNFSLYNTPVLDFIQIRQKVRTDDLLIIMHGNKAYANGLKAVLEAFPGHILC
                     FSTWEEKRKKLGNRKPSIWILSEKLAEEADPTGNSQDASRVILLTGKEWLPKEKAGLT
                     VLTMPLKPEALRRAVKEHLQ"
     gene            1021..1194
                     /locus_tag="ACZPCG_11545"
     CDS             1021..1194
                     /locus_tag="ACZPCG_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285718.1"
                     /translation="MQNMIITVLAGIILAAAIRLRKKNRELGRLKEQIHQVKEETKAL
                     QAQVQTLRIRISD"
     gene            1511..1675
                     /locus_tag="ACZPCG_11550"
     CDS             1511..1675
                     /locus_tag="ACZPCG_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285719.1"
                     /translation="MIFSAIVLAFGSRPDTALWETLTEKEGGYRIGDGLKAGDAKKAI
                     YEVTRLALEL"
     gene            1725..2489
                     /locus_tag="ACZPCG_11555"
     CDS             1725..2489
                     /locus_tag="ACZPCG_11555"
                     /inference="COORDINATES: protein motif:HMM:NF014101.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HIR1285720.1"
                     /translation="MAYAINEPKEVSTMPRPKKCRKVCQMPQIREFHPLGRACGDPAV
                     ILTVDEYEAIRLIDRQGFSQEECSAYMQVARTTVQLIYNSARKKIAEALVEGRVLRIE
                     GGDYQLCSGEEEYCGCGGCKKHRLGCAQKLTKEEKRMKIAVPLDENRQDVCIVLARAP
                     YFMFRENGRDTIVENPAAKALGGAGIQAAQFLVDSRIDTLLTVRCGENAAEVFKAAGI
                     KIYKSANKAAADDLAALEEGRLEELTHFHGGYHGVQ"
     gene            2486..3310
                     /locus_tag="ACZPCG_11560"
     CDS             2486..3310
                     /locus_tag="ACZPCG_11560"
                     /inference="COORDINATES: protein motif:HMM:NF013793.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1285721.1"
                     /translation="MKIACLSGKGGAGKTLAAVNLATAAGNAVYIDCDVEEPNGHLFL
                     KPDRTEEVTVSALLPEFDPGRCTGCKKCVEFCRFHALMYIREKPMVFSEVCHSCGGCS
                     LVCPENAVTEREKPVGRLQIGMAGDVKTVTGILNPGEASGIPVIREALKQAEGLTIID
                     GPPGSACSVMESVMDADFCILVAEPTAFGFHNFQMVYELVTLLGRKCGVIINKEETPY
                     EPLEDFCREHDLKILCRIPYSPRIGAMAAAGELVSRKDPELGRLFADVLKQIGGVL"
     gene            3307..4188
                     /locus_tag="ACZPCG_11565"
     CDS             3307..4188
                     /locus_tag="ACZPCG_11565"
                     /inference="COORDINATES: protein motif:HMM:NF013793.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HIR1285722.1"
                     /translation="MKHLLILSGKGGTGKTTTAAAFIHFSQTRAMADCDVDAPNLHLV
                     AAPPVSPEISEFLGGEKAVVDPDKCVGCGICASHCRFNALEKEEGRYRVNEYACEGCG
                     VCAYVCPQKAVYLAEDVAGRKELYLGERIFSTAVLKMGRGNSGKLVTDVKMAMLKHAP
                     ETELAIIDGSPGIGCPVIASVSGMDLVLIVAEPSQSGISDLKRLVKTARTFQASLAVC
                     VNKWDVSPEHARDIKEFCKEEDIPFAGVIPYDKNVSLAVNAGKSIADIDCPARDALKN
                     IFETVNQILKKDEKGVR"
     gene            4191..4553
                     /locus_tag="ACZPCG_11570"
     CDS             4191..4553
                     /locus_tag="ACZPCG_11570"
                     /inference="COORDINATES: protein motif:HMM:NF014620.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HIR1285723.1"
                     /translation="MKIAVAAMGSEVAGHFGHCENFIFFDAEDGKIVAENSVPNPGHR
                     PGFLPNFLADNGAQVIISGGMGGGAVDIFNERGVEVIVGVQGDARTAVETYLRGELIS
                     TGSICHNHEHADECGEHH"
     gene            4655..5488
                     /locus_tag="ACZPCG_11575"
     CDS             4655..5488
                     /locus_tag="ACZPCG_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIR1285724.1"
                     /translation="MVQIITDSSVMYTEDEAKAMGFEAIPLCIHIGDLEGRDLQIDME
                     EFYRRIQEGQVPRSSQPPIGEVLDAYARYPQDEIVHISIAEGLSGTYHGACSAREMTE
                     NPERITVFNSRTLCGPHRYMTEQAQKMKEEGRSASEILEWLKEAAQKTESFLIPQDFS
                     FLKRGGRLTPVAAALGSVLKLKPVMKLTEDGTRIDNFFVKRTMSAAVKGIMEYMKQKD
                     IGQNHILYIVHAAAKKEAENIRTMFETAFAGLEIHILELSPVFVAQGGPKCVAIQYIH
                     R"
     gene            complement(5566..>5718)
                     /locus_tag="ACZPCG_11580"
     CDS             complement(5566..>5718)
                     /locus_tag="ACZPCG_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HIR1285725.1"
                     /translation="GIYTAMGGGTSMWLGILFVCFLLPAVLTFIFGEALRKMGWIKDG
                     DLKLDL"
BASE COUNT         1594 a         1144 c         1589 g         1391 t
ORIGIN      
        1 cttagatgaa ggctgaccat atattctccc tgattatgta aaaataaact gaaataatca
       61 tatatcaatc gatgtaaaaa tgcaagatac acctacagct taatctcaac cctgtaatca
      121 tcatcgatca atctaaagtt tacatgatgc gcctgagcca gccttagaac ctgggcgttt
      181 tccagattgt gtccagattt attatttgcg gggggattgc ctttccggag cttgtaaaac
      241 gatacaggaa cgggcgggtc atgctggaca ggcagaaaaa tcctactcgc gatgtgggga
      301 cacagtatat ccgctggttt gatgaatacg caggcagctt ctttaaggta tctgccaggg
      361 atgagcgtcc ctatatctgc ggggaacgct gctggcagct tcggtgtgaa tacctccatc
      421 aaaataaagg atttttaaat gatgaaaatg aggatacagt tcattttcac ctaggtgtaa
      481 actgtggcac atctctctgt gaactggaca gaacaagagg ccaggcagac aggagcgaca
      541 ttcggattga tatcgagcag ttttgtctga ggatgtatca ggcggcaaaa agctactatg
      601 accaggttca taaggaaaaa aatttcagcc tttacaatac gccggttctc gattttatcc
      661 agatccgtca gaaggtacga acggatgacc tgcttattat catgcatgga aacaaagctt
      721 atgccaatgg acttaaggct gttttagaag cgtttcccgg tcatatcctt tgtttttcca
      781 cctgggaaga gaaaagaaaa aaactgggaa atcggaagcc atctatctgg atcctgtcag
      841 agaaattggc agaggaggca gatccaaccg ggaatagcca ggatgcatcc agggtgattc
      901 ttctgaccgg aaaagaatgg ctgccgaagg agaaggccgg cctgacggtt cttactatgc
      961 cattgaagcc ggaggcttta cgcagagcgg taaaagaaca tttgcagtag aggagataag
     1021 atgcagaata tgatcataac tgttttggca ggaattattt tggcagcagc catccgtctg
     1081 cgtaaaaaaa acagagagct tgggcggtta aaggagcaga ttcatcaggt gaaggaggag
     1141 acgaaagcgc tgcaggctca ggtacagacc ctccggataa ggatttccga ttaggaatct
     1201 gcggaggacg agctgtggga ttgtgcaaat accattcatc tgtatgcagc actttctaaa
     1261 gaggaagcga ggcagccgtc tttcagggaa aagcaggaag agattttaca ggcagatgtt
     1321 taaaatgtct gcggcttcct gaagcgtgga tgtttcctta agaactcttt gcagcggtct
     1381 gagcggcact gctgcagctg ctcagtgcat tttttataag tcgggtttga aataatcatt
     1441 ctggcctgcg gcgctcttac gattgttccg gacatcccgg gaataaaaca ggataatgta
     1501 tatctggtaa atgatattct cagccattgt tcttgccttt ggttccagac cggatacagc
     1561 cctttgggaa actctgacag agaaagaagg gggatatcgt attggagacg gccttaaagc
     1621 aggagatgca aagaaagcaa tctatgaagt aaccagattg gcattggagc tgtagaaaat
     1681 gttattgaca tatgccgtaa acagagttat actccaaaat ataaatggca tatgccataa
     1741 acgagccgaa agaggtgagt acgatgccaa ggcccaaaaa gtgccgaaag gtgtgccaga
     1801 tgccgcagat aagggaattt catcctttgg gcagagcctg cggtgatccg gcagtgatcc
     1861 tgactgtgga tgaatatgag gcaatccgcc ttatcgacag acaggggttt tcccaggagg
     1921 aatgcagtgc ctatatgcag gttgccagaa ctacggttca gcttatctat aattccgccc
     1981 ggaaaaagat agcagaggca ctggtagagg gacgggttct gcgcattgag ggcggggact
     2041 atcagctttg cagcggagaa gaagagtact gcggctgcgg aggctgtaaa aaacaccgtc
     2101 tgggctgtgc acaaaagctg acgaaggagg aaaaaaggat gaaaatcgca gttccgttgg
     2161 atgaaaacag acaggatgtg tgtattgttt tggcccgcgc accctatttc atgttccgtg
     2221 aaaacggcag ggatacaatc gtggaaaatc cggcagccaa agcattggga ggcgcaggga
     2281 ttcaggcagc gcagtttctg gtggacagcc gtatcgatac tctgcttaca gtccgctgcg
     2341 gagagaatgc ggcagaggtc tttaaggccg caggtataaa aatttataaa tccgcaaata
     2401 aagccgctgc ggatgatctg gctgcattgg aggagggaag gcttgaggag ctgactcact
     2461 tccacggcgg ctaccatggt gtacaatgaa aatcgcctgc ttgagcggca agggcggtgc
     2521 ggggaaaact ctggctgcag tgaatcttgc aacagcagcc ggaaatgcag tatatattga
     2581 ctgtgatgtg gaagagccca acggacattt atttctgaag cccgatcgga cagaggaggt
     2641 aacagtaagc gctcttttgc cggagttcga tcccggccgc tgcaccggct gtaaaaaatg
     2701 cgtggaattc tgccgcttcc atgctctgat gtatatcagg gaaaaaccca tggtattttc
     2761 tgaggtctgc cacagctgcg gcggctgcag tctggtttgc ccggaaaatg ctgttacaga
     2821 aagagaaaag ccggttggaa ggctgcaaat cgggatggcg ggagatgtga aaacagtgac
     2881 cggtatttta aatcccggag aggcgtcagg catccctgtt attcgggaag cgcttaaaca
     2941 ggcagagggg ctgacgatta tagacggccc tcccggaagc gcctgcagtg tgatggaaag
     3001 tgtgatggat gcggacttct gtattcttgt ggcagagcct acagcctttg gctttcacaa
     3061 tttccagatg gtttatgagc ttgtgactct gttggggaga aaatgcggcg ttattatcaa
     3121 taaagaagag actccctatg aaccgctgga ggatttttgc agggagcatg atcttaaaat
     3181 actgtgccgt atcccctatt ctcccaggat cggagccatg gcggcagcag gagaactcgt
     3241 ttcccgaaaa gaccctgagc ttggcagact gtttgcagat gtgctgaagc agattggagg
     3301 tgtcttatga aacatctttt gatcctcagc ggaaagggag gcactggaaa gactaccacc
     3361 gcagcggctt ttattcattt ttctcagaca agggcaatgg cagactgcga tgtggatgcg
     3421 cctaaccttc atctggtagc ggctcctccg gtttctccgg aaatttccga atttctggga
     3481 ggggagaagg ctgtggttga tcctgataaa tgtgttggct gcgggatctg tgcgtcccac
     3541 tgccggttta atgctcttga aaaggaagag ggaagatatc gggtaaatga atatgcctgc
     3601 gagggctgcg gtgtgtgcgc ttatgtatgt ccccaaaagg cagtgtatct tgcggaggat
     3661 gtggcaggca gaaaggaact gtatctgggc gagaggatat tttctacggc tgttttgaaa
     3721 atgggccggg gaaattccgg aaagctggta acagatgtga agatggccat gctgaagcat
     3781 gctccggaaa cggaactggc cattattgac ggatctcccg gcattggatg cccggtgatt
     3841 gcgtctgtca gcggaatgga tctggtgctt atagtggcag aacccagcca gtcgggaatc
     3901 agcgatttga aacggctggt aaaaaccgcc agaacatttc aggcaagttt ggcagtctgt
     3961 gtcaataaat gggatgtaag tccggaacat gcgcgggata taaaggagtt ctgcaaagaa
     4021 gaggatattc cgtttgcagg cgtgattccc tatgataaaa acgtatcatt ggcagtgaat
     4081 gcaggaaaaa gcattgcaga tatagactgc cctgccagag atgcgttaaa aaatatattt
     4141 gaaactgtaa accagatctt aaagaaagat gaaaaaggag taagatgatt atgaaaattg
     4201 cagtagcagc tatgggcagc gaggttgccg gacattttgg acattgtgag aactttatct
     4261 tttttgatgc agaggacggt aagattgtgg ctgaaaattc cgtccccaat ccgggccacc
     4321 gtcccggctt tctgccgaat ttcctggctg ataacggagc ccaggtaatt atctccggcg
     4381 gaatgggcgg aggagctgta gatatcttta atgagcgcgg tgtagaggtg attgtaggtg
     4441 ttcagggtga cgccagaact gcagtagaaa cctatcttcg gggcgagctg atttctaccg
     4501 gatcgatctg ccacaatcat gagcatgcag atgagtgcgg cgagcatcac taaagattga
     4561 aaaatgattc tccgcctatt gacattttta tacaaatatt gcaaactata ataagtagtt
     4621 tctgaaacta tattataggg cggaggaatt agatgtggta cagattataa cggattcttc
     4681 tgtgatgtat acagaagatg aggcaaaagc aatggggttt gaggcaattc ccctgtgcat
     4741 acatatagga gatctggaag gccgggatct tcagattgat atggaggaat tttaccggcg
     4801 tattcaggaa gggcaggttc ccagatcctc tcagcctccc atcggggagg tgctggatgc
     4861 atatgccaga tatccgcagg atgagattgt ccatatttcc atagcagaag gcctttccgg
     4921 cacctatcac ggagcatgca gcgccagaga aatgacagaa aaccctgagc ggattacggt
     4981 gttcaacagc agaactctgt gcgggcctca tcgatatatg acagaacagg cgcagaagat
     5041 gaaggaagag ggaagaagtg cttctgagat tctggaatgg ttaaaggagg ctgcccaaaa
     5101 gacagagtcc tttttgatcc cgcaggactt ttccttttta aagagaggcg gaaggctgac
     5161 tcccgttgca gcggcccttg gttctgtttt gaaattaaag ccggttatga agctgacgga
     5221 ggatggaacc agaattgaca atttttttgt aaagcgtacc atgtctgccg ctgtaaaagg
     5281 aattatggag tacatgaaac agaaggacat cgggcagaac catattttat atattgtcca
     5341 tgctgcggcg aaaaaagagg cggagaatat tcgtactatg tttgaaaccg catttgcagg
     5401 tctggaaatt catatactgg agctgagtcc ggtgttcgtg gctcagggag ggccgaagtg
     5461 tgtggcaatt caatatattc accgttaaat tttgaatgta tttgtaccgg tatataaaaa
     5521 ctcctggctg attaccctgt acgggatcag tcaggagttt ttagtttaca gatccagctt
     5581 taaatcgccg tccttaatcc agcccatttt ccgaagcgct tctccgaaaa taaaggtcaa
     5641 tacagcgggg aggaggaagc agacgaaaag gattcccagc cacatactgg ttccgcctcc
     5701 catggcagtg tagattcc
//
LOCUS       DBMDUH010000167         5671 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_5633_length_5671_cov_5.503232, whole genome shotgun sequence.
ACCESSION   DBMDUH010000167 DBMDUH010000000
VERSION     DBMDUH010000167.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 5671)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5671)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5671
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5633_length_5671_cov_5.503232"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1557
                     /locus_tag="ACZPCG_11585"
     CDS             <1..1557
                     /locus_tag="ACZPCG_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990112 - RQC complex [Evidence IEA];
                     GO_function: GO:0001968 - fibronectin binding [Evidence
                     IEA];
                     GO_process: GO:0072344 - rescue of stalled ribosome
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein"
                     /protein_id="HIR1285726.1"
                     /translation="NNKPSPLTAPNFCMLLRKHIGSARILSITQPGLERILEFELEHL
                     DEMGDLCRKKLIVEIMGKHSNIIFCKEDGTIIDSIKHVSANMSSVREVLPGREYFIPQ
                     TVAKENPLTVTEADFCRIIKAAPMTVQKALYNHFTGISPIVAEEVCHLASIDSDLSAN
                     GLSEAELTHLAHTFCLLMEDVKEGRFSPAIIYDEEGPLEYASIPLTCLEGRGCRREGW
                     SSISALLENYYASRDSRTRIRQKSADLRRIVQTALERNYKKYDLQLKQLKDTEKREKY
                     RIYGELLNTYGYELAGGEKSFSCINYYTGEEISIPLDPQLSAKDNAKKHFDKYNKLKR
                     TFEALTQLTDQTKAEIDHLESISSALDIALAENDLVQIKEELMEYGYVKKRRPNDKRP
                     KITSKPFHYISSDGYHIYVGKNNYQNEELTFRFANGNDWWFHAKGIPGSHVILKNDGK
                     GELPDRAFEEAGALAAFYSKGRENEKVEIDYIQKKNIKKVTGAAPGFVIYHTNYSLVA
                     IPSCNLKEAE"
     gene            complement(1705..1881)
                     /gene="rpsU"
                     /locus_tag="ACZPCG_11590"
     CDS             complement(1705..1881)
                     /gene="rpsU"
                     /locus_tag="ACZPCG_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HIR1285727.1"
                     /translation="MSNVIVKDNESLDSALRRFKRNCAKAGIQQEIRKREHYEKPSVR
                     RKKKSEAARKRKYN"
     gene            complement(2045..4693)
                     /gene="alaS"
                     /locus_tag="ACZPCG_11595"
     CDS             complement(2045..4693)
                     /gene="alaS"
                     /locus_tag="ACZPCG_11595"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HIR1285728.1"
                     /translation="MKEPQYRGVNELRRMFLEFFESKGHLAMKSFSLVPHNDNSLLLI
                     NSGMAPLKPYFTGQEIPPRRRVTTCQKCIRTGDIENIGKTARHGTFFEMLGNFSFGDY
                     FKTEAIHWSWEFLTEVVGLDPDRLYPSIYVDDDEAFEIWNKEIGIPADRIFRFGKEDN
                     FWEHGSGPCGPCSEIYYDRGEKFGCGKPGCTVGCECDRYIEVWNNVFSQFDNDGHGNY
                     SELKQKNIDTGMGLERLAVVVQGVDSLFTVDTNKALLDRVCALSGKEYQKEHETDVSL
                     RIVTDHIKSCTFMISDGIMPSNEGRGYVLRRLLRRAARHGRKLGIEGKFLADLSKTVI
                     DLSKDGYPELEEKQSMIFKVLSEEEDKFNKTIDQGLAILAEMEASMVSAGEKTLSGEN
                     AFKLYDTYGFPLDLTREILEEKGCGVDEEGFNAAMKEQKDKARKARKATNYMGADVTV
                     YQSIDPAITSEFVGYDKLSVDSKISVLTTEEELVEALTDGQTGTIITDETTFYGTMGG
                     QQGDKGIISNENGEFKVKDTIHLQGGKIGHVGVMTKGMLRVGEAVTLSVCERNRALTC
                     RNHSATHLLQKALRTVLGSHVEQAGSYVDAGRLRFDFTHFSAMTPEEIKKVEDMVNAE
                     ISASLPVETQVMTLDEAKKTGAMALFGEKYGEKVRVVKMGDFSTELCGGTHVANTSAI
                     ASFKILSEAGIAAGVRRIEALTSDGLMKHYAEVEEELHEAAKAAKTTPASLTSRIEAL
                     LEEIKALSSENEKLKSKLANDSLGDVMSQVKEVNGVKVLASRVADVDMNGLRNLGDQL
                     KDKLGEGVVVIASVMDGKVNLMATATEAAQKKGVHAGNLIKAIAGLVGGGGGGRPNMA
                     QAGGKNPAGVDEALKKAVETVEEQTK"
     gene            4911..>5671
                     /locus_tag="ACZPCG_11600"
     CDS             4911..>5671
                     /locus_tag="ACZPCG_11600"
                     /inference="COORDINATES: protein motif:HMM:NF013703.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1285729.1"
                     /translation="MNQSPSQTTHLFTNRQITAMLLPLMAEQLLITLMGMVNTFMVAH
                     VGSYAISAVSLVDSINILVIQAFYALSTGGTIVCAQYLGQKKTGHAIRSAEQTLFIVT
                     LISLVTAAFCILFDRQILRLIFGTVDEEVFNSARTYFFYSSLSYPFIALYDAGSSIFR
                     AQKNTGFPMAMSLIANIANVIGNIILVLILGQGVAGAAISTLAARALCALLVLLRLVK
                     PRENQPVYVANYTRIRPHMPLIRRILHIGIPAGIE"
BASE COUNT         1365 a         1523 c         1281 g         1502 t
ORIGIN      
        1 aataataaac ccagccctct gaccgccccc aatttctgta tgcttttaag aaagcatatc
       61 ggaagcgcca ggatcctttc catcacccag ccggggctgg agcgtatcct ggaatttgag
      121 ctggagcatc tggacgaaat gggcgacttg tgcagaaaaa agctgatcgt agagatcatg
      181 ggaaagcaca gcaatatcat tttctgtaag gaagacggca cgatcattga cagcatcaag
      241 catgtgtccg ccaatatgag ttctgtaagg gaggttctgc ccggaaggga atactttatc
      301 cctcaaacag tagcaaaaga gaatcctctg accgtcacgg aagcggattt ctgccggatc
      361 ataaaagccg cgcccatgac ggttcagaag gccctgtaca atcatttcac aggcatcagc
      421 cccattgtgg cagaggaggt ctgtcatctg gcttccatcg attccgacct gtctgccaac
      481 gggctgtctg aggcagaact gacccatctg gcccatacct tctgcctgct gatggaggat
      541 gtaaaggaag gacgcttctc tcctgctatt atctatgatg aggaaggccc tctggaatac
      601 gcttccattc ccctgacctg tctggaagga aggggctgcc gcagagaggg ctggtcttcc
      661 atcagcgctc ttctggaaaa ttactatgcc tccagagaca gccgcacaag gatccgccag
      721 aaatccgccg acctgcgccg gatcgtccag accgccctgg agcgcaatta taaaaaatac
      781 gatctgcagt taaagcagct aaaggatacg gaaaagcggg aaaagtaccg gatctacgga
      841 gaactgttaa atacctacgg ctacgagctg gcaggcggtg agaaatcctt ttcctgcatc
      901 aactattaca caggggaaga aatctctatc cccctggatc ctcagctttc tgcaaaggac
      961 aatgcaaaga agcattttga caagtataat aaattaaaga ggacctttga ggccttaacc
     1021 cagctgaccg accagacaaa ggcggaaatc gaccatctgg aatccatcag ctctgccctg
     1081 gatatcgctc tggctgaaaa tgatctggtg cagatcaagg aggaactgat ggaatacggc
     1141 tatgtgaaaa agcgccgtcc aaacgataag cggccaaaga tcaccagcaa gcccttccat
     1201 tatatttcct ctgacggata ccacatctat gtgggcaaaa acaactatca gaatgaggag
     1261 ctgaccttcc ggtttgcaaa tggaaatgac tggtggttcc acgccaaggg catacccggc
     1321 tcccatgtga ttttaaaaaa tgacgggaaa ggcgagctgc cggaccgggc ctttgaggag
     1381 gcgggcgcac tggctgcctt ctattccaag ggccgcgaaa atgaaaaagt ggaaatcgac
     1441 tatatccaga aaaaaaatat caaaaaggtt acaggagccg caccgggctt tgtgatctac
     1501 cacaccaatt attctttggt tgcgatacct tcctgcaatt tgaaagaagc agaatagcga
     1561 aacaggcccc gattttcaca ccggggcctg tcgtttttta tcgtatcatt ttatactgtc
     1621 acaaataaaa cagccagcca tcggaatctc tcccgacagc cggctgattt actttactca
     1681 aaatcaacta atcccgtgcg tgaattagtt atacttgcgc ttacgagcag cttcagactt
     1741 cttcttacgt cttacgcttg gcttctcgta atgctctctc ttacgaattt cctgctgaat
     1801 gccagccttt gcacagtttc ttttgaatct gcgtaaagca ctgtctaagc tctcgttatc
     1861 tttaacaatt acgtttgaca tcgctcacac ctaacctccc tccagttgtg taattgtgca
     1921 taatacaact gtttgggtat ttttagcact gctatgatta tagcataaaa atcccatacg
     1981 tcaagaaaaa aattatattt ttttcttatt tttatttcca cagtaaactt tcgggcaaaa
     2041 tggattattt tgtctgctct tctactgttt caaccgcttt cttaagggcc tcgtctactc
     2101 ctgccggatt ctttccgcct gcctgagcca tgttgggacg gcctccgccg cctccgccta
     2161 ccagaccggc aatggccttg atcagattgc ctgcgtgaac gcccttcttc tgagcagcct
     2221 ccgttgcggt agccatcaga tttacctttc catccattac agatgcgatt acaactacgc
     2281 cctcgcccag cttatccttt aactggtcgc ccaggtttct caggccgttc atatctacat
     2341 ccgctactct ggaagccaga acctttacgc cgtttacttc ttttacctgg ctcattacgt
     2401 cgcccaggga gtcatttgcc agcttgctct ttaatttttc attttcagag ctcagagcct
     2461 taatttcctc taaaagagcc tcaatccggg aggttaagga tgcaggagtg gtcttggctg
     2521 cctttgccgc ctcatgaagc tcctcttcca cctctgcata gtgcttcatc agtccgtcgg
     2581 aggtaagagc ctcaatacgt cttacgcctg ctgcaatgcc tgcttcagaa aggatcttaa
     2641 aggaagcaat ggcgctggta tttgccacat gggtaccgcc gcaaagctct gtggagaaat
     2701 ctcccatctt taccacgcgg accttctcgc cgtatttttc accgaagagg gccatagcgc
     2761 ctgtcttctt tgcctcatcc agggtcataa cctgtgtttc aaccggaagg gaagcgctga
     2821 tctctgcatt taccatatct tccacctttt tgatctcctc aggagtcatg gcggagaaat
     2881 gggtaaagtc aaaacggagt ctgcctgcgt ccacatagga gcctgcctgc tctacatggc
     2941 ttccaagaac ggttcttaaa gccttctgaa gcagatgggt cgcactgtgg tttctgcagg
     3001 ttaaggcgcg gtttctctcg catacggaca aagttacggc ttctccaacg cggagcattc
     3061 cctttgtcat cacgcctaca tggccgatct ttccgccctg aaggtgaatg gtgtccttaa
     3121 ccttgaactc gccattttca ttggaaatga tacccttatc gccctgctgg ccgcccatgg
     3181 tgccatagaa agtggtttca tctgtgataa tggttccggt ctggccgtct gtaagcgcct
     3241 ctacaagctc ctcctctgta gtcagaacgg aaatcttaga atccacgctc agcttgtcat
     3301 agcctacgaa ttcagaggta atagccggat cgatggactg gtatacggtc acgtctgcac
     3361 ccatgtagtt ggttgccttg cgggccttgc gggccttgtc cttctgctcc ttcatggcag
     3421 cattaaagcc ttcctcatca acgccgcagc ccttttcctc caggatctct cttgtcagat
     3481 ccagagggaa gccgtaggta tcgtaaagct taaatgcatt ttctccggat aaggtctttt
     3541 caccggcaga aaccatggaa gcctccatct cggcaagaat ggcaagaccc tggtcgatgg
     3601 tcttattaaa cttatcctct tcctcgctca ataccttaaa gatcatggac tgcttctctt
     3661 ccagctccgg ataaccgtcc ttggaaaggt cgattacggt ctttgaaagg tctgccagga
     3721 actttccttc gattcccagc ttgcgtccat ggcgggcagc gcggcgcagc aggcggcgca
     3781 gcacatagcc tctgccctcg ttggaaggca tgatgccgtc ggaaatcata aaggtgcagg
     3841 acttgatatg gtctgtgaca atacgcaggg aaacgtcggt ctcatgctcc ttctggtatt
     3901 ccttgccgga cagggcgcat acgcggtcta agagagcctt gtttgtatcc accgtaaaca
     3961 gggaatctac gccctgaacc actacagcca ggcgctccag tcccataccg gtatcaatat
     4021 ttttctgctt taattcggaa taattgccgt ggccgtcatt gtcaaactgg ctgaatacgt
     4081 tgttccaaac ctcgatatag cggtcgcact cgcagcctac ggtacatccg ggttttccgc
     4141 agccgaactt ctcgcctctg tcatagtaga tctcagagca ggggccgcag gggccggaac
     4201 cgtgctccca gaagttgtcc tcttttccaa agcggaaaat acggtctgca ggaataccga
     4261 tttccttatt ccagatctca aaagcctcgt cgtcatctac atagatggag gggtacaggc
     4321 ggtccggatc caggccgact acttctgtta aaaattccca ggaccagtga atggcctctg
     4381 tcttaaagta atcgccaaag gagaagttgc ccagcatctc aaagaaggtg ccgtggcggg
     4441 ctgtctttcc gatattttca atatcgccgg tgcggatgca cttctggcag gtagtaactc
     4501 ttcttctggg agggatctcc tgtccggtaa agtaaggctt taagggagcc ataccggagt
     4561 tgatcagcag aaggctgttg tcattatgag gcacgagtga aaagctcttc attgccagat
     4621 gtcccttgct ctcaaaaaat tcaaggaaca tacggcgcag ttcgttcact ccgcggtact
     4681 gtggttcttt cactttctct tcctccatat cctttgacgc gcaatatacg cgcatcttaa
     4741 tttgaaatca taaccataag cgacatttta ccatattgac ttgtatttat caagatattt
     4801 gccctctatt tcgttattat ctccattgtg gcacgtttcg gagtatgcta cacttactct
     4861 cagaataata tggcgccgca gcgaatgcgc cggaaaggat ccttttcccc atgaaccaat
     4921 ccccatccca aaccacccat ctttttacca accgtcagat caccgccatg ctgcttcccc
     4981 tgatggctga acagcttttg atcaccctta tgggcatggt aaataccttt atggtagccc
     5041 atgtaggctc ttatgccatt tccgccgtat ccctggtgga ttccatcaat attctggtga
     5101 tccaggcctt ttacgccctg tctaccggcg gaaccattgt ctgcgcccag tatctggggc
     5161 aaaaaaagac agggcatgcc atccgctctg ctgaacagac cctgtttatt gtaacgctga
     5221 tctctcttgt aacagctgca ttctgcatcc tgtttgaccg gcagatttta aggctgatct
     5281 tcgggacagt ggatgaagaa gtatttaact ctgctcgtac atactttttc tattcatccc
     5341 tctcctaccc atttatcgcc ctgtatgatg ccggttcctc catcttcaga gcacagaaaa
     5401 ataccggctt tcccatggcc atgtccctga tcgcaaacat tgcaaatgtg atcggaaata
     5461 tcattctggt gctgatcctg ggacagggtg tagcaggcgc cgccatctcc actctggctg
     5521 ccagagcctt atgtgctctg ctggttctct tgcgccttgt aaagcccaga gaaaatcagc
     5581 cggtctatgt agccaattat acccgcatac gcccccatat gcctttgatc cggcggattc
     5641 tccatatcgg tattcccgca ggcattgaaa a
//
LOCUS       DBMDUH010000168         5632 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_5673_length_5632_cov_4.807991, whole genome shotgun sequence.
ACCESSION   DBMDUH010000168 DBMDUH010000000
VERSION     DBMDUH010000168.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 5632)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5632)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5632
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5673_length_5632_cov_4.807991"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="ACZPCG_11605"
     CDS             <1..212
                     /locus_tag="ACZPCG_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285730.1"
                     /translation="LLYYHDSGISREELLDMMYGSGEYANPAGSLRALVFRLRRIMEE
                     CLPAGSISRRREAFTAGSAKALAYM"
     gene            134..1060
                     /locus_tag="ACZPCG_11610"
     CDS             134..1060
                     /locus_tag="ACZPCG_11610"
                     /inference="COORDINATES: protein motif:HMM:NF015649.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HIR1285731.1"
                     /translation="MSSCRKHIQTEGGVYRWVSKSVSVYVDARDFQATAEKGLEKENE
                     EELLRACGLYQGEFLAQLAGEKWVTIVSVRCQELYFRCLRSLSRQMQENREYQSLLNI
                     VNNACQLYPYEECQIMKMDCLIAMKRFREAMQVYKWVVRQYFEEQGLPPSEKMLERFR
                     TMSSQIRYTTDMLKDVRESLKERDAVNGPYYCSYPSFIDSYRLLNRLSERISFEKVLI
                     CIFFVDIRGKALDDGNDLLKEASSQLRKAVGISLRKGDLFTCYSPSQYLILLSGADQE
                     ECQNIFQRIEQNLRCWEDWRRIRLKYEVLSQV"
     gene            complement(1063..2235)
                     /locus_tag="ACZPCG_11615"
     CDS             complement(1063..2235)
                     /locus_tag="ACZPCG_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR1285732.1"
                     /translation="MLNQFIFQNYRIFQNETLLDFFPAPINEHKRSLLTAPGDQETFL
                     PVIALYGPNGCGKSSVLEALWGLCRFMDKDCSLILSSSRSSCRLDPEAAKKPISFDLL
                     FRNGSFLFRYQLQILKGDIKEEHMFYGKPGTEDAGVLFSRTGNDIHLGTAAGGLVLTG
                     VPSAAPLLPILSGRSGTPCIQAAAGWFSQADFFRSNRTNDVPLLPEKEEKKGRLCRLL
                     QNMDIDILDYSYRKESGFSQSSLFLTHGRPGHPTFAVSYEEESEGIRKLLCLLPPVLK
                     SLDCGGLLLADDLDQALHPHLLRYLISLYKNREKNPHNAQLVFTAHNTAILTPGVMRR
                     DEIYLCCRPEGKDAVLYPLSSYKKENGLIPRNDEAYGKQYLEGRYGAAPKLTEADA"
     gene            2641..>5632
                     /locus_tag="ACZPCG_11620"
     CDS             2641..>5632
                     /locus_tag="ACZPCG_11620"
                     /inference="COORDINATES: protein motif:HMM:NF021028.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HIR1285733.1"
                     /translation="MEGQKRPRVFLQKRYNTQKRFLALMLAFAMIFTSVGTDLNVAYA
                     AEGNRVDFEIYGADLVEAINDAVETQSPVTADDLNFTNGAIEKFDALFFGEGKVYEVF
                     PPVEGGDMDAEVRVFVRLPEDADDMYMVTGDEEVIFLYINNGEDTISCSTKIIRTVDG
                     EEKVKSTKRITVKSYEDKFGEEESNIISKPEETLPAVPETSAPESQPEETPAETLNPA
                     ETTAPEETTIPEETEAPDATEESSETEETTEAPESTEAQETETSEETKEEVTEAETEA
                     TEAEKEETEAVEETEEPETETDEEKSQDDSAVTLSSISRHQVPVVAVKEDAELIETEE
                     VSETEAEEETEKETEAETEETEAEETEKETTAAPEKETTAAEETTSKETTAAPEESTE
                     ATEETSTEAESSEEATESEEATTAPETQETEETTEAETEVPAETETTAPAETETQAPV
                     QSATPAEPEEGSKPAATDTDLVGMGWCSTAKAYTTTLKALKVFESQIELTADVAGAEN
                     VTVTLSAMPGVVPEGSYVEAEAITDEDQLNLMKKEADKKLMKKNLAAVDIFAADVMLY
                     DADGNEIWPDGNVKVTFEGTGIDGGSSTVYHINEGSTYAKIEAEETYYYEVNFYYNNG
                     QEDVLISGVQYVKEGEMAIVPDAPEQEGKNFVGWEPELGPITEDTNFYAQYAENAEQI
                     HLTVNYQYKDGSSAAQPWVAEVQAGVKCDYTVESPSIDGFEPDQKEVVFKDAYDTDQT
                     VTVTYTGAEGRYHIEHWLLDVQGNKTGEPAETETAVGEVGNYTQAVSKEYEGFTAREI
                     SQVKIGSDGNTVVEVLYERNSYTLTWNTGEGGSYIAPSEIVYGAAVETPAKDPSRLGY
                     DFAGWENLPATMPAQDTVVTAKWNPAQKADYKVIYWKETLTKGEYAVGEITYGKGGFV
                     GNNIPYSTQKNYEGFKLNETKSEGNVEITPDGMAVKNVYYDRETYTIKFMSKQRDKLE
                     SPCDKRKTRTVANGTGLVFGAPYLT"
BASE COUNT         1731 a         1117 c         1534 g         1250 t
ORIGIN      
        1 tcctgctgta ttatcacgac agcggcatca gccgggagga actccttgat atgatgtacg
       61 ggagtgggga atatgccaat ccggcgggga gtctcagagc gcttgtattc cgtctccgcc
      121 gtattatgga ggaatgtctt cctgccggaa gcatatccag acggagggag gcgtttaccg
      181 ctgggtcagc aaaagcgtta gcgtatatgt agacgccaga gactttcagg caacagccga
      241 gaaaggactg gagaaagaaa atgaggaaga actcctccgt gcctgcggac tttatcaggg
      301 ggaatttctg gcacagctgg ccggagaaaa gtgggttaca attgtaagtg tccgctgcca
      361 ggaactttat ttccgctgcc tgcgcagcct cagccgtcag atgcaggaaa acagagagta
      421 ccagtccctt ttgaatattg tcaataatgc ctgccagctt tatccctatg aagaatgtca
      481 gatcatgaaa atggattgtc tgatcgccat gaagcgtttc agggaggcaa tgcaggtgta
      541 taaatgggtg gtgcgtcagt attttgaaga acagggactt ccaccttcag aaaagatgct
      601 ggaacgtttc cgcactatga gcagccagat ccgttatacg acagatatgc tgaaggatgt
      661 gcgggaaagc ttaaaggaac gggatgcagt caacggtccc tattactgct cctatccctc
      721 ttttattgac agctaccgtc ttttaaaccg gctttccgag cggatttctt ttgaaaaggt
      781 gcttatctgt atattttttg tagatataag gggaaaagcg ttagatgatg gaaatgatct
      841 tctaaaggaa gcctccagtc agctgagaaa ggctgtggga atctccttgc gcaagggaga
      901 tctgtttacc tgctacagtc caagtcagta tctgatcctg ttaagcggag cagatcagga
      961 ggagtgtcag aacatttttc aacggatcga gcagaatctg cgctgttggg aagactggcg
     1021 cagaatccgc ttgaaatatg aagttttgag tcaggtctga attcaggcat ctgcctctgt
     1081 aagttttggg gctgctccat agcggccttc cagatactgt tttccatagg cctcgtcatt
     1141 tcttgggatg aggccgtttt cctttttata ggaggagagc gggtagagga cggcatcttt
     1201 tccctcagga cggcagcaca gataaatctc gtccctgcgc atcactccgg gagtgaggat
     1261 cgccgtattg tgggcagtga aaacaagctg cgcattgtga ggattttttt cacggttttt
     1321 atacagagat atgagataac gcagcaggtg ggggtgaagg gcctgatcca gatcatcagc
     1381 cagcaaaagc cctccgcagt ctaggctttt aagaaccggt ggaagaaggc agaggagctt
     1441 tctgatcccc tcagattctt cttcatagga caccgcaaag gtgggatggc ccggacggcc
     1501 gtgggtgaga aagagggagg actgagaaaa gcccgattct tttcggtagg aatagtccag
     1561 tatatcaata tccatatttt gtaaaagacg gcacagccgt cctttttttt cttctttttc
     1621 cggcagcaga gggacgtcgt tcgttctgtt tgaacggaaa aagtcagcct gggagaacca
     1681 tcctgcggca gcctggatac agggagtgcc agaacgtcct gacaaaatcg gaagaagggg
     1741 agcggcagag ggaactcctg taagaaccag tccgccggcg gcagttccca gatggatatc
     1801 gtttcctgtg cggctaaaga gcacgcctgc atcctccgta ccgggttttc cgtaaaacat
     1861 atgctcctct ttaatgtctc cctttaaaat ctgaagctgg tatcggaaaa ggaagcttcc
     1921 atttctgaaa agcaggtcaa aggaaattgg cttttttgct gcttccggat ccagacggca
     1981 gctgctgcgg gagctggaga gaatgagaga gcagtccttg tccataaatc ggcacagtcc
     2041 ccaaagagct tccaggacag aggacttccc acagccattt ggcccataca gagcgatcac
     2101 ggggaggaag gtttcctgat cccccggagc ggtcagaaga gagcgtttgt gttcattgat
     2161 gggggcaggg aaaaaatcaa gaagcgtttc attttgaaaa atacggtaat tttgaaaaat
     2221 aaactgattc agcatagcag taaccctcca aaaacatgag aaaaaaactc ataaaataca
     2281 gctgatttta tattaacaca gcagaaaaac aaatgcaata aatgagaaaa aatctcacaa
     2341 aaattccgcc agggagggaa tgaaacgaat gggttattcc tatctgcagc gttgaatata
     2401 agataagata gagaaaatat aatgagaaaa aattcaatgg taaaaaatag aaatataacg
     2461 cctggtataa cgcggtatct gttatgatag ggttaagcaa attttagaag gaaaatggaa
     2521 ataaattaat ctttttttaa tgaaacctta atttgtaacc agattgtaac tcctgtatgg
     2581 tatgatttgc ttatacggac cggggaggcc gtaaaaatct taagagagag gagaaaaagt
     2641 atggaaggac agaaaagacc aagggtgttt ctgcagaagc gctataacac ccaaaagcgt
     2701 ttcctggcgc tgatgctggc gttcgcgatg atctttacca gtgtgggtac agatctgaac
     2761 gtggcctatg cagcagaagg aaacagagtc gacttcgaga tctacggagc agatctggtg
     2821 gaagccatca atgatgcagt agaaacccaa agcccggtga cagcggatga tctgaacttt
     2881 accaatggag ccattgaaaa gttcgatgcc ctgttctttg gcgagggcaa ggtctacgaa
     2941 gtatttcccc cggtggaagg cggagatatg gacgcagaag taagagtgtt cgttcgtctt
     3001 cctgaggatg ccgatgatat gtatatggtc acaggcgatg aggaagtcat cttcttatac
     3061 ataaataacg gagaagacac cattagctgt tccacaaaga tcatccgcac ggtagatggg
     3121 gaagaaaagg tcaagagcac caagcggatc acagtcaaga gctatgaaga caaatttggt
     3181 gaagaagaga gcaatatcat ttcaaagccg gaagaaacat tgccggctgt gcctgaaaca
     3241 agtgctccgg aaagtcagcc ggaggaaacc ccggccgaaa cactgaaccc ggctgagaca
     3301 acagctcctg aggagaccac catccctgag gaaacggaag ctcccgatgc gacagaagaa
     3361 tcttccgaga cagaagaaac aaccgaagcg ccggagagca cagaggctca ggaaacagaa
     3421 acctccgaag aaacaaagga agaggttaca gaggctgaaa cagaagccac agaggcagag
     3481 aaagaggaaa cggaggctgt tgaggagact gaagagccgg agacagagac tgatgaggaa
     3541 aaatctcagg atgattccgc agtaaccctt tcctccattt cccgtcatca ggtacctgtg
     3601 gtagctgtga aggaagatgc agagctgatc gaaacagagg aagtttccga gactgaagct
     3661 gaggaagaaa cagaaaagga gaccgaggca gagaccgagg aaactgaggc tgaagaaacc
     3721 gagaaggaaa ctacagctgc tcctgaaaaa gagacaacgg ccgctgagga aaccacttcc
     3781 aaagaaacaa ccgcagcgcc ggaggaaagc acagaagcaa cagaggaaac ctccacagag
     3841 gctgaaagta gtgaagaggc tacagagtct gaggaagcta caactgctcc tgaaactcag
     3901 gagacagagg aaactacaga agctgagacc gaagttcctg ccgagacgga gacgacagcg
     3961 cctgctgaga cagagactca ggcacccgtt cagtcggcaa ctccagctga accggaagag
     4021 ggaagcaagc ccgctgccac agataccgac ctggttggta tgggatggtg cagtacggcg
     4081 aaggcgtata cgacaacttt aaaggcttta aaggtatttg aaagtcagat tgagcttaca
     4141 gcagatgtag ccggtgctga aaatgttacg gttacactga gcgcaatgcc tggagtagtt
     4201 ccggaaggaa gttatgttga ggcagaggcg attacagatg aagatcagct gaatttgatg
     4261 aagaaggaag ctgataaaaa gcttatgaag aaaaatttgg ctgcagtaga tatctttgca
     4321 gctgatgtta tgctttatga cgcagacggc aatgagattt ggccggacgg aaatgtaaag
     4381 gttacatttg agggaaccgg aatagacggt ggttcttcga ctgtatacca tataaatgaa
     4441 ggttccacgt atgctaagat tgaagctgag gaaacctatt attatgaagt aaacttctat
     4501 tacaacaatg gacaggaaga tgtacttatc tctggcgttc agtatgtaaa agaaggtgaa
     4561 atggcaatcg taccggatgc tcctgaacag gaaggaaaga attttgtggg atgggagccg
     4621 gaattgggcc cgatcacaga agacacaaac ttttatgctc agtatgcaga aaatgctgaa
     4681 cagatccatt tgacagttaa ctaccagtat aaggatggtt catctgcagc acagccgtgg
     4741 gttgcagagg ttcaggcagg tgtaaaatgt gattataccg tagaatcacc gtcaattgat
     4801 ggatttgagc cagatcagaa agaagtcgta tttaaggatg catatgatac agaccagaca
     4861 gtaacagtaa catatacggg tgctgagggc agatatcata ttgagcattg gttattagat
     4921 gttcagggaa ataaaaccgg agagccggca gagacagaga cagcagttgg tgaggtagga
     4981 aactatactc aggctgtatc taaggagtat gagggcttta ctgcaagaga gatttctcag
     5041 gtgaagattg gctctgacgg caatacggtt gtagaggttc tgtatgagcg aaatagctat
     5101 actttgacct ggaataccgg agagggtgga agttatatcg caccgtctga aatcgtatat
     5161 ggtgcagcag ttgaaacacc tgcaaaagat ccgagcagat taggatatga ttttgcaggc
     5221 tgggagaatc tgcctgcaac aatgcctgcg caggatacag tagtgacagc caagtggaat
     5281 cctgctcaaa aagcagacta caaggttatc tactggaagg aaactctgac aaagggtgag
     5341 tatgcagtag gtgagattac ctatggtaaa ggcggattcg ttggaaacaa tatcccatat
     5401 tctacacaaa agaactatga gggcttcaag ctgaatgaga ctaagtcgga agggaatgta
     5461 gaaatcacac ctgatggtat ggcggtaaag aatgtatatt atgaccgcga gacgtatacc
     5521 attaagttta tgagtaaaca gagagacaag ctcgaaagtc cctgtgacaa aagaaaaacc
     5581 cgcaccgtgg cgaacggtac gggtctagtg tttggtgccc cttatctgac tc
//
LOCUS       DBMDUH010000169         5558 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_5744_length_5558_cov_5.251787, whole genome shotgun sequence.
ACCESSION   DBMDUH010000169 DBMDUH010000000
VERSION     DBMDUH010000169.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 5558)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5558)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5558
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5744_length_5558_cov_5.251787"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            87..926
                     /locus_tag="ACZPCG_11625"
     CDS             87..926
                     /locus_tag="ACZPCG_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012200743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1285734.1"
                     /translation="MRTKRRTVFDVISVILFVMISLIMLIPIYKVLVDSFDLKTAYGM
                     RLLPQQFGLDGYISVFTNPTLFQPFLISCVTTIAGTFAGLAISTLGAYVLIQWKMPGR
                     KLLANFLLFTMIFNGGMIPTYLVMKTLGLTNTLWVVILLPAINVYNLVLMRNFFEGIP
                     ASLFESAEIDGCSPMGTFFKIVLPLSKAALTAIGLMFAVQYWNDYTNYKLYITNENLY
                     NFQMKLRSLILGSDLPSAVGSATENTIKNAAVIVAILPFVIIYPFCQKYFISGVNIGA
                     VKE"
     gene            949..2502
                     /locus_tag="ACZPCG_11630"
     CDS             949..2502
                     /locus_tag="ACZPCG_11630"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006771411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HIR1285735.1"
                     /translation="MNTGTYKNPILYADYSDPDAIRVGEDYYMVASSFTYLPGVPLLH
                     SRDLVHWEIMNYCVPALPFEKYDRPSHGSGTWAPSIRYHNGEFFVFVPLPDEGIFVAR
                     SKDPKGTFQLNRLCEAKGWIDPCPFWDEDGRAYMIFAYARSRCGIKHRLALVEMDPEC
                     TQLKGEPKVIFDGTQLAPTSEGPKLYKKDGWYYVLMPSGGVASGWQSALRAKKIWGPY
                     EYKIVMHQGNTSVNGPHQGAWVDTPEGSSWFIHFQDVGELGRIVHLQPMCFQDGWPFI
                     GCEKNGDMIGEPVEEWEMPATGQPAFRIADSDEFETGRPGLQWQWQANPDEAFFARER
                     EMGTLKLACLANSERENLLWYAPNVMTQIPQSNAFTADVKLRLSEGAGTEDSDGDMAV
                     LGMTGHHYAFCGLEREGGAVWLRLYEGVVTGKEFEGEAMEGLAASREWKQDKALWLRM
                     ELREDKTFSFSVSDDGSTFLPFGPSFHLAKGTWTGAKLCLWACNRNNTVSGGWGEYDY
                     IHIRHKKEC"
     gene            2465..4267
                     /locus_tag="ACZPCG_11635"
     CDS             2465..4267
                     /locus_tag="ACZPCG_11635"
                     /inference="COORDINATES: protein motif:HMM:NF016497.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HIR1285736.1"
                     /translation="MITYTSDTKKSAEEAFQAALLGDLDKVKQLAGHTFHLETCDEKG
                     NSLLHYAVRSKNLELVRFLTEAGGLDLTWANADLVTPFDLAHIQAKEYPGDLAAEEME
                     AWIASVCGFSHEACYRNPVVRGMYPDPSIVRVREDYYMVNSSFIFFPGIPVSHSRDLV
                     HWETIGYVAADPDWARKYLGPLEGGRGFWAPDISYHDGWFTVCATLRMNDDADCIQTQ
                     FVARSRKPEGPYEKPVIHKVLGIDPSIFHDEDGRTYMLLNRGARIMEISEDGKECLSE
                     PELIWYGWDKHGPEGPHILKKDGWYYCFLAEGGTGIGHKITVARSRQLMGPYEDCPYN
                     PIMTQKDPAAAIQCCGHGKPVSTPDGRWYMVYLCSRYLDGKWGILGRETCLDEITWTP
                     DGWPLVNQRKGPSYMAKLPLNGLQKPDPVKLPYDGWLCPRTIDRERSFVSPEGILRIR
                     GEGKDLNDRSCVSLLVKRQPDFNFTCEYEMWAPDEDGYRDAGLTLYYDENTFIKFGIK
                     DRCLFVREYVDNAYVRCLEQDFEPGSSHVYLKIVTEGLKRSFYINGTVVGVLDHVTSL
                     CSEGLKKGKRFTGAMTGVYNNGTNFICWRQHRSI"
     gene            4224..4880
                     /locus_tag="ACZPCG_11640"
     CDS             4224..4880
                     /locus_tag="ACZPCG_11640"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012200747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HIR1285737.1"
                     /translation="MERILYAGDSTVAFNKINTYPQTGMSQALPLYLKDNIQVISFAK
                     NGRSTRSFIAEGRLAAIEKELKKGDYLFVQFGHNDSKADEARHTDPDTDFKENLRMFA
                     ETAKACGAKAVFITPIARRLFDENGTFKPGSHGAYPRAMKEAAKEAGVPVVDLTTVTE
                     QYLAQVGELWSRPLFVWPKDNTHLKYEGAVMMAGFLAEGLRALGEPYSSLLAEEKEEQ
                     "
     gene            4882..>5558
                     /locus_tag="ACZPCG_11645"
     CDS             4882..>5558
                     /locus_tag="ACZPCG_11645"
                     /inference="COORDINATES: protein motif:HMM:NF012869.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HIR1285738.1"
                     /translation="MDRYQEMERRFAACYQKHGKHSVRVLLEFYRGQYHKFLLSTLFF
                     LLKHSPALLSPLLIANVVNGVIEGGEAGSRAIFINVGIWLGLLSIHLPANWIHNQLKS
                     RVIRNTEAGLRAALVRKLQELSIPYHTGSQSGRLQSKIMRDVEAVETLSSQLFVNLLN
                     IVMNLVITLSITAVKNRLILVFFILVAPVAAGAVVAFRAKIHRENRSFRQGMEETSAR
                     VMEMVEMV"
BASE COUNT         1357 a         1275 c         1562 g         1364 t
ORIGIN      
        1 gttgtaggct gtattctggt gctgctgtgc aaccgggcaa gctataaggt ccgcggcaga
       61 ggcattgtat aggaggaata aaaattatga gaacgaaaag aaggactgtt ttcgatgtga
      121 tttcggtcat cctgtttgta atgatcagcc tgatcatgct gatccccatt tataaggttc
      181 tggtggattc ctttgattta aagacggctt atggtatgcg gcttctgccg cagcagttcg
      241 gcctggacgg atatatttct gtatttacca atcccactct gttccagccg tttctgattt
      301 cctgtgtgac gaccattgcg ggaaccttcg caggacttgc catttccact ctgggcgctt
      361 acgtgctgat ccagtggaag atgccgggac gtaagctgct tgcaaatttc cttttgttta
      421 ccatgatctt taacggcggt atgatcccta cctatctggt tatgaagacc ctgggcctga
      481 ccaataccct ctgggttgta atcctgcttc ccgcgatcaa cgtgtataat ctggtgctga
      541 tgagaaactt ttttgaggga atcccggcca gcctgtttga gtctgcggag attgacggct
      601 gttctcccat gggaacgttc tttaagatcg tccttccctt atccaaggct gctttgacgg
      661 ccatcggact gatgtttgcg gttcagtact ggaatgatta caccaattat aaactttaca
      721 ttacaaatga aaacctgtat aatttccaga tgaagctgcg ctccctgatc ttaggaagcg
      781 atcttccttc cgcagtggga agcgcaacgg agaacacaat taagaatgcg gctgttatcg
      841 tagccattct tccgtttgtg atcatttatc cattctgcca gaaatacttt atttccggcg
      901 tgaatatcgg cgctgtcaaa gagtgacaga cgcagggagg agaaatgtat gaacactgga
      961 acttacaaaa atccaatttt atatgcagac tactccgatc cggatgcaat ccgggtcggg
     1021 gaagactatt acatggtggc atcttcgttt acatatcttc cgggggtgcc tctccttcat
     1081 tccagggatc tggtccactg ggagatcatg aactactgcg tgccggccct gccgtttgaa
     1141 aaatatgacc ggccatccca tggctcggga acctgggcgc cctccatccg gtatcataac
     1201 ggggagtttt ttgtctttgt gcctctgccg gatgagggga tttttgtggc gcgctccaaa
     1261 gacccgaaag gaacctttca gctgaaccgt ctctgtgaag ccaaaggctg gatcgatccg
     1321 tgtccgttct gggatgagga tggaagagcc tatatgatct tcgcctatgc aagaagccgc
     1381 tgcgggatca agcaccgtct ggctctggtg gagatggatc cggaatgtac acagctaaag
     1441 ggagagccaa aggtgatctt tgacggcact cagctggcgc ccaccagcga aggccccaaa
     1501 ctgtacaaaa aggatggctg gtattatgtg ctgatgcctt ccggcggtgt tgcctccggc
     1561 tggcagtctg ccctgcgggc aaagaagatc tgggggccct atgagtataa aattgtaatg
     1621 catcagggaa atacatcggt caacggccct catcagggtg cctgggtgga tacgccggaa
     1681 ggaagcagct ggtttatcca ttttcaggat gtaggagaat tagggcgcat cgttcatctg
     1741 cagcccatgt gcttccagga tgggtggccc tttatcggct gtgaaaaaaa cggagatatg
     1801 atcggagagc cggtagaaga gtgggagatg cctgcgacag gccagcccgc cttccggatc
     1861 gcggattcgg atgaatttga aaccggacgt ccggggctgc agtggcagtg gcaggccaat
     1921 ccagacgaag ctttttttgc acgggaacgg gaaatgggaa cccttaagct ggcctgtctg
     1981 gccaactcgg agagggagaa tcttctgtgg tatgctccca atgtgatgac ccagatccct
     2041 cagtccaacg catttacggc agacgtaaag cttaggctgt cggagggcgc tgggacagag
     2101 gacagtgacg gagatatggc cgttttgggc atgacgggcc atcactatgc gttctgcggc
     2161 ctggaacggg aaggcggagc ggtctggctg cgtctttacg aaggcgttgt cacaggaaaa
     2221 gagtttgaag gcgaggcgat ggagggactg gcagcctcaa gggagtggaa acaggataaa
     2281 gctctgtggc tgcgcatgga gcttagggaa gataagactt tttctttttc agtttctgac
     2341 gacggcagta ccttccttcc cttcggccct tccttccatc tggcaaaggg gacctggacc
     2401 ggggccaagc tctgcctgtg ggcctgcaac cggaacaata ccgtatcagg aggatgggga
     2461 gaatatgatt acatacacat cagacacaaa aaagagtgct gaagaagcct ttcaggcggc
     2521 tcttttgggc gatctggaca aggtaaaaca gctggcaggc cataccttcc atctggaaac
     2581 ctgtgatgag aaagggaaca gccttcttca ttatgcggta agaagcaaaa atcttgagct
     2641 ggtgcgtttc cttacagaag cgggagggct ggatctgacc tgggccaatg cagatctggt
     2701 aacgcccttt gatctggctc atatccaggc caaggagtat ccgggcgatc tggcggcaga
     2761 ggaaatggaa gcctggattg cttccgtctg cggcttttcc catgaagcct gctaccgcaa
     2821 tccggtggtc cgggggatgt acccagatcc cagcattgtg cgtgtgagag aagactacta
     2881 catggttaat tccagcttta tcttttttcc gggaattccg gtgtcccatt ccagagatct
     2941 ggtccactgg gaaaccattg gatatgtggc ggcggatccg gactgggcca ggaagtatct
     3001 ggggccgtta gagggcggaa gaggcttctg ggcgccggat atcagctatc atgacggctg
     3061 gtttaccgtc tgtgccaccc ttcggatgaa tgatgacgcg gattgtatcc aaacccagtt
     3121 tgtggcccgt tccagaaagc cggaaggccc ttatgagaaa ccggtgatcc acaaagtttt
     3181 aggaatcgat ccctccattt tccatgatga ggacgggcgc acctatatgc ttcttaaccg
     3241 cggagcacgc atcatggaga tctctgagga cgggaaagaa tgtctttcag aaccggagct
     3301 gatctggtat ggctgggata agcatgggcc tgaggggcct catattttga aaaaggacgg
     3361 ctggtattac tgtttcctgg ctgagggtgg aaccgggatc ggacacaaaa ttacggtggc
     3421 ccgctcccgg cagcttatgg gcccctatga ggactgtccc tataacccga tcatgaccca
     3481 gaaggatccg gctgcagcca tccagtgctg cggccatgga aaaccggtca gtactccaga
     3541 cggacgctgg tatatggtat atctctgttc gcggtatctg gacggaaaat ggggaatttt
     3601 gggaagagaa acgtgtctgg atgagattac ctggacaccc gatggctggc cgctggtaaa
     3661 tcagagaaaa ggcccctctt atatggcaaa gcttcctctt aatggattgc agaaaccgga
     3721 tccggttaag cttccttatg acggctggct ctgtccaaga accattgaca gagagcgtag
     3781 cttcgtttct cccgaaggaa tccttcgtat ccggggagaa ggaaaggact taaatgacag
     3841 aagctgcgtc agccttctgg taaaacgcca gccggatttt aactttacct gcgaatatga
     3901 aatgtgggct ccggatgagg atggataccg ggacgccgga cttaccttat attatgatga
     3961 aaacacattt ataaaatttg gcataaagga tcgctgcctg tttgtcagag aatatgtgga
     4021 caatgcctat gtccgctgtc tggagcagga tttcgagccg ggaagcagcc atgtttacct
     4081 taaaatagtc acagaagggc ttaagcggtc tttctatatc aatggaaccg ttgtcggcgt
     4141 tttggatcac gttacatccc tttgttcaga gggattaaaa aaaggaaaac gctttacagg
     4201 agcaatgaca ggagtgtata acaatggaac gaattttata tgctggagac agcaccgtag
     4261 catttaataa gatcaataca tatccccaga ccggtatgag ccaggctctt ccgctgtatc
     4321 tgaaggataa tatccaggta ataagctttg caaaaaacgg ccgcagcacc aggtccttta
     4381 ttgcagaggg acggcttgcc gccattgaga aggaacttaa gaagggggat tatctgttcg
     4441 tccagttcgg tcacaatgat tcaaaagcag atgaagcgcg tcacaccgat ccggatacgg
     4501 attttaagga aaatcttcgg atgtttgcag agacagcaaa ggcctgcggt gcaaaggccg
     4561 tatttattac ccccattgcc agacgtctgt ttgatgaaaa cggaactttt aaaccgggaa
     4621 gccacggtgc ctatcccagg gcgatgaaag aggcggcaaa ggaagcaggc gttccggttg
     4681 tggatcttac tactgtgaca gaacagtatc tggcacaggt gggggaactg tggtctcgtc
     4741 cgctgtttgt gtggccaaag gacaataccc atttaaaata tgaaggggcc gttatgatgg
     4801 ccggattcct tgcagagggc cttcgggcat tgggagagcc atacagcagc cttctggcag
     4861 aggagaagga ggagcagtaa aatggatcgg tatcaggaga tggagcgccg ctttgcagcc
     4921 tgttatcaaa agcatgggaa gcactctgta cgggtgcttc tggaattcta caggggacag
     4981 tatcacaaat ttcttctctc cactctcttt ttcctgctga aacattcccc ggccctcctt
     5041 tcccctcttt tgatcgcaaa tgtagtcaat ggggtgatcg agggaggaga agccgggagc
     5101 agggcaattt ttattaatgt gggaatctgg ctggggcttt tgagcatcca tctgcctgca
     5161 aactggatcc acaatcagct gaaaagccgt gtgatccgaa atacggaagc cggacttcgg
     5221 gcggctctgg taagaaaact ccaggagctg tccattcctt atcatacggg cagtcagtcg
     5281 ggacgccttc agtcaaagat catgcgtgat gtggaggcgg tggaaaccct gtcatcccag
     5341 ctttttgtca atctgcttaa tattgtgatg aatctcgtta tcacactgtc tatcacagcc
     5401 gtaaaaaaca ggctcatcct tgtgtttttt attctggttg ctccggtggc tgccggagcg
     5461 gtagtggcat tcagagccaa gatccacagg gaaaaccgct ccttccgtca gggaatggag
     5521 gaaaccagcg caagggtgat ggagatggta gaaatggt
//
LOCUS       DBMDUH010000170         5314 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_5997_length_5314_cov_5.596585, whole genome shotgun sequence.
ACCESSION   DBMDUH010000170 DBMDUH010000000
VERSION     DBMDUH010000170.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 5314)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5314)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5314
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5997_length_5314_cov_5.596585"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            641..2020
                     /locus_tag="ACZPCG_11650"
     CDS             641..2020
                     /locus_tag="ACZPCG_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1285739.1"
                     /translation="MKKELMKRVAAAGLAVSMVAGLTACGPTGKPEETKKEETKAEGG
                     ETAAAADKEEAAASDGEVTLRFSWWGGDARHKATEAACQAFMEKYPNIKVECEYGAWD
                     GWAEKVATQLSGGTAPDLMQVNWNWLYQFSSDGSKFVDLTQFADVINMENYPANLLEQ
                     CVVGGKQQAIPIGTTGKCFYWNKTTFDKAGIALPTSWDELIAAGTTFKEKLGDEYYPL
                     AMYEYERMLLMMYYLEGKYGKEWAENNTLNYSLEEVKEGLEFINSLEDAHVLPSIAQL
                     KGDGATVLEKNPGWINGQYAGFYEWDSAQEKFANSLEGDQEFVLGEFLKGGEYEAGLT
                     KISSCFAITETSEHKKEAAMLLDFLINDPKGIELMGTERGMLANTYANEVLLGLGKLE
                     GLTYEGNQAVMKVANFALDPNFENSALKDSTGIYYEVFEGLSSGQDVEMLAQYLIDSI
                     NEVYASNPY"
     gene            1992..3155
                     /locus_tag="ACZPCG_11655"
     CDS             1992..3155
                     /locus_tag="ACZPCG_11655"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88/105 protein"
                     /protein_id="HIR1285740.1"
                     /translation="MKFMQAIHIKLGKAVIMENREIINAYIDKWIEDYNSRQRAVWNY
                     EDGCVMLGAQYLYEATGDEKYINCIRTFMDRYIQEDGTIRYYVPEDYNLDKVNNGRVL
                     YFLYDKTGEEKYKTALDHIRGQLETHPRTDCGSFWHKQIYPYQIWLDGLYMGQPLYLE
                     YEKRFNGGERYNDITGQFLNARKYLFDEEKKLYYHAYDEKRQMIWADKTTGCSHNFWL
                     RSIGWYLMALIDCCGLIPEESFEHLSTYKRLFKEALAGIMQYQDEKTKLFYQLVDMPE
                     LEGNYLETSGSAMIAYAILKGCRLGILQEEKWRRRGEEIFDGLEREKLQKDEDGWHLT
                     GICLVAGLGPKDERDGSVEYYLSEPVVSDEEKGVGVFMMAYGEYLKLEKQDEA"
     gene            3171..4076
                     /locus_tag="ACZPCG_11660"
     CDS             3171..4076
                     /locus_tag="ACZPCG_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1285741.1"
                     /translation="MQKKTKKMTRASRQNIGYAYIAIWIIGFLVFKFYPFMSSLFYSF
                     TNYNLFKESVENIGFANYEKIVDTAKYLKSYIVTFKYAFMTVPLKLLFALFIAYLLNF
                     KIKGINLFRTAYYIPSILGGSVAIAVLWQFLFQNEGLVNTIIGLFGVKPVNWLGSPDY
                     ALFVICLLRVWQFGSAMVIFLAALKGVPQDLYEAASIDGAGKFTQFLKVTIPLITPVI
                     FYNLVTQLCQAFQEFNGPFMITKGGPLGSTTLISILIYQNAFKTYDMGLASAMAWLLF
                     FIVAVLTAVSFISQKHWVYYGDEER"
     gene            4085..4933
                     /locus_tag="ACZPCG_11665"
     CDS             4085..4933
                     /locus_tag="ACZPCG_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1285742.1"
                     /translation="MTRQQKYKIQTIARYIILIAVGLVMIYPMIWMVGASFKATNAEI
                     FGSIGFIPKEFTLDGYKNGWFASTYQFGRYMVNTYKFVIPKVIGTVVSSAIAAYGFSR
                     FQFKARGFWFALMLSTLFLPQVVLNVPQFLLFTKWGWVDSYLPLVVPSFFACDTYFVF
                     MLVQFMRGISRELEEAAEIDGCNSFKRFIYVVLPMVQPSLVSCALFQFMWSSNDFMGP
                     LIYLNSTKKFTASLGLRMIMDTESGFDWNKTLALSVITLIPSLVIFFLAQDQFVEGIA
                     AGGVKG"
     gene            4962..>5314
                     /locus_tag="ACZPCG_11670"
     CDS             4962..>5314
                     /locus_tag="ACZPCG_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006775651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HIR1285743.1"
                     /translation="MKFQIIFQTSRKVTIEFLDFGIYFTEKKYRLLLNGKEILTSDRV
                     VQTISGLTPDTEYELTAVADGEAEETVRFKTDYEFVTLNVRRFGARGDGKHDDTVAIQ
                     AAVSSCPEHGRVYIP"
BASE COUNT         1540 a          967 c         1316 g         1491 t
ORIGIN      
        1 gtattcagag gtcctgttgc atacactttt tggggtgtta caatttccag tacagtgggc
       61 atttgcgttg tcagaccaat ctggtttgca aaagtggcgc cagaaaaata tccataggtt
      121 tctccggcat tttttatata ttttcgtata ataactttca ttggatctaa atagcttttt
      181 ttcaggatac gggatgcctt gggaagataa taaaccccgg tatcaaaccg aataatatct
      241 cctgactgaa ccattctctt gaagtattgc cgcagagcat tatcgctgat accttctaac
      301 tgaatttcac tgataaaaat aggctcatta tatccatagg ctttttctcc atgatactat
      361 ttttatacta tataagtcaa taaatatgtc aaaatatagt ttggatgatt tgaaaaggat
      421 ttattcatag gcggtgaaaa aggtatgtca aaccggagaa tagatataga aaatgtatgt
      481 atacatatat aaaataatag tgtatacata caaaataaac aataatctat tgacattgca
      541 taaactatgt actaatataa aactacaaat aaaagggagt gggctgtttt tgtgagaaac
      601 agctgcattc cggaacaatg tgtgaaacag gaggaagaaa atgaaaaagg aattgatgaa
      661 gcgtgtggca gcagcaggtc tggcagtatc tatggttgca gggcttacag cctgcggacc
      721 tacaggaaag cctgaagaga ccaagaagga agagacaaag gctgagggcg gagagactgc
      781 cgcagctgcc gataaggaag aggccgcagc ttctgacgga gaagttactc tgcgtttcag
      841 ctggtgggga ggcgatgcca gacataaggc aacagaggca gcatgtcagg catttatgga
      901 aaaatatcct aatatcaagg tagagtgcga gtacggagcc tgggacggat gggctgagaa
      961 ggtagctacc cagctcagcg gcggtacagc acctgatctg atgcaggtta actggaactg
     1021 gctgtatcag ttctccagcg acggttctaa attcgtagat ctgacccagt tcgcagatgt
     1081 aatcaatatg gaaaactatc cggcaaatct tctggagcag tgtgtagtag gcggcaagca
     1141 gcaggcgatt ccgatcggta ctacaggaaa gtgcttctac tggaataaaa ccacctttga
     1201 caaagccggt attgcccttc ctacaagctg ggatgagctg attgcagcag gaaccacatt
     1261 taaggagaaa ttaggcgatg agtactatcc tctggctatg tatgaatatg agagaatgct
     1321 tctgatgatg tattatctgg aaggaaaata cggaaaagaa tgggcagaga acaatacctt
     1381 aaattattct cttgaggaag taaaggaagg tcttgaattt attaattcct tagaggatgc
     1441 acatgttctt ccaagcattg ctcagttaaa gggcgacggc gctacagttc tggagaagaa
     1501 tccgggttgg atcaacggac agtatgccgg cttctatgag tgggattctg ctcaggagaa
     1561 atttgctaac tccctggaag gcgatcagga gtttgtactt ggtgagttcc ttaagggcgg
     1621 agagtatgag gcaggactta caaagatttc ttcctgcttt gcaattacag aaacctctga
     1681 gcacaaaaaa gaggctgcta tgctgttaga tttccttatc aatgatccaa agggtattga
     1741 gcttatgggc acagagcgcg gtatgctggc taacacttat gcaaatgaag ttctgttagg
     1801 tcttggcaag ctggaaggcc ttacctatga gggcaatcag gcagttatga aagttgcaaa
     1861 ctttgcactt gatccaaact ttgagaattc cgcattaaag gattctacag gtatctacta
     1921 tgaggtattt gagggactca gcagcggaca ggatgtagag atgctggcac agtatctgat
     1981 tgacagtatc aatgaagttt atgcaagcaa tccatattaa gcttggaaag gcagtaatca
     2041 tggaaaacag agaaataata aatgcttata tagataagtg gattgaagat tataattcca
     2101 gacagcgtgc tgtctggaat tatgaagatg gctgtgttat gttaggagcc cagtatctgt
     2161 atgaagccac cggagatgaa aagtacatca actgcatccg gacatttatg gaccggtata
     2221 tacaggagga tggaaccatc cgctactatg ttcctgagga ttacaatctg gacaaggtaa
     2281 acaacgggcg ggtgctgtat ttcctttatg acaaaacagg ggaagaaaag tataaaactg
     2341 cgctggacca tatcagagga cagctggaaa cacatccccg tacagactgc ggaagcttct
     2401 ggcataagca gatctatccg tatcagatct ggctggacgg attatatatg gggcagcctc
     2461 tttatctgga atacgaaaag agatttaacg gcggagagcg ctacaatgat attacaggcc
     2521 agtttttaaa cgccagaaaa tatctgtttg atgaagagaa aaaactgtat taccacgcat
     2581 atgacgagaa gcgccagatg atctgggcag acaaaacaac aggctgctcc cacaatttct
     2641 ggctgcgctc cattggatgg tatctgatgg cgctgattga ctgctgcggc ctgattcccg
     2701 aggagagctt tgaacatctg agtacatata agcggctgtt taaagaagct ctggcaggaa
     2761 ttatgcagta tcaggatgaa aaaacaaagc tgttttatca gctggtagat atgcctgagc
     2821 tggaaggcaa ttatctggaa acgtctggat ccgctatgat tgcatatgcg attttaaagg
     2881 gttgccgtct gggtatttta caggaagaaa aatggcgcag gagaggcgaa gagatctttg
     2941 acggcctgga aagagaaaag ttacagaagg atgaagatgg atggcatctg acgggcatct
     3001 gtctggtggc tggattaggc cccaaggatg agcgggatgg aagcgtagaa tactatcttt
     3061 ccgaaccggt ggtttcagat gaagagaagg gcgtaggagt tttcatgatg gcctacggcg
     3121 aatatctgaa gctggaaaaa caggatgaag catagaatgg agaatgcctt atgcagaaaa
     3181 agacaaaaaa aatgacccgt gcatccagac agaacatagg atatgcatat attgctatct
     3241 ggatcatcgg ttttctcgtt tttaaatttt atccgtttat gtcctctctt ttctatagct
     3301 ttacgaacta taatctgttc aaggaatctg tagaaaatat aggatttgca aattatgaga
     3361 agattgtcga tacggcaaaa tacttaaagt cctatattgt aacctttaag tatgccttta
     3421 tgacagtacc cttaaaactg ctgtttgcgc tgtttatcgc atatcttctc aactttaaga
     3481 tcaagggaat taaccttttc cgtacagcgt attatatccc gtctattctg ggaggttcag
     3541 ttgcgattgc tgttttatgg cagttccttt tccagaatga aggtctggtt aacactatca
     3601 tcggcctgtt tggagtaaag cctgttaact ggctgggaag cccggattat gcactgtttg
     3661 taatctgcct tctccgtgta tggcagtttg gatcagctat ggttattttc ctggctgcat
     3721 taaagggcgt tccgcaggat ctgtatgaag cggcgtccat tgacggagca ggaaaattta
     3781 cacagttttt aaaggttacc attccgctga ttacaccggt tattttctac aatctggtta
     3841 ctcagctgtg tcaggcattc caggagttta atggaccgtt tatgattacc aagggaggcc
     3901 ccttaggttc tacaaccctg atttccatcc tgatctatca gaatgcattt aagacatatg
     3961 atatgggact tgccagtgct atggcatggc tgctgttctt tattgttgca gtgctgacgg
     4021 cagtttcgtt tatcagtcag aagcattggg tgtattatgg tgatgaagaa aggtaggaat
     4081 taaaatgaca agacaacaaa aatacaaaat tcagaccata gcacgctaca taattctgat
     4141 tgcagtgggg cttgtaatga tctaccccat gatatggatg gtaggagctt cctttaaagc
     4201 aaccaatgcg gagatttttg gctccatcgg ttttattccg aaggagttta cgctggacgg
     4261 ctataaaaac ggctggttcg ccagtaccta tcagtttggc agatatatgg tcaataccta
     4321 taaatttgtt ataccaaagg taataggaac cgttgtttcc tcagccattg cagcatatgg
     4381 tttttcacgg tttcagttta aggcccgggg cttttggttt gcactgatgc tttccacgct
     4441 gtttctgcct caggtagtgt taaatgtgcc tcagttctta ttgtttacaa aatggggctg
     4501 ggtggattcc tacctgcctt tagttgtgcc gagctttttt gcctgtgata cctactttgt
     4561 atttatgctg gttcagttca tgagaggaat ttcaagagag ctggaggagg ctgcggagat
     4621 tgacggctgc aattccttta agcgtttcat ttacgtagtg cttcccatgg ttcagccttc
     4681 actggtatcc tgcgcgctgt tccagtttat gtggtcctcc aatgacttca tgggtccgtt
     4741 aatttactta aattctacaa agaaatttac agcttctctg ggcctgcgta tgatcatgga
     4801 tacggaaagc ggatttgact ggaataagac gctggcatta tctgttatta cactgattcc
     4861 atccctggtt atattcttcc tggcgcagga tcagtttgtg gaaggtattg cagccggagg
     4921 agtaaagggt taaagacgcg aattgtaaga aaggacgcaa catgaaattt cagattatat
     4981 ttcagacatc cagaaaggtc acgattgaat ttttggattt tggaatttat tttacagaaa
     5041 agaagtaccg tctgttgtta aatggaaaag aaattcttac ttccgatcgg gtagttcaga
     5101 ccatttccgg tctgacgcct gatacggaat atgagcttac tgctgtggca gatggggaag
     5161 cagaagagac ggttcgtttt aagacagatt atgaatttgt aaccctgaat gtacgccgtt
     5221 tcggagcgag aggcgatgga aagcatgatg atacagttgc tattcaggcg gcggtgtcat
     5281 cctgtccgga gcacggaaga gtgtatattc ccaa
//
LOCUS       DBMDUH010000171         5308 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_6002_length_5308_cov_4.825043, whole genome shotgun sequence.
ACCESSION   DBMDUH010000171 DBMDUH010000000
VERSION     DBMDUH010000171.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 5308)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5308)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5308
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6002_length_5308_cov_4.825043"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="ACZPCG_11675"
     CDS             <1..428
                     /locus_tag="ACZPCG_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIR1285744.1"
                     /translation="ILGGFYGGLGGAIGMTIGDLFDPVYVIYAPKTFILKLCIGLITG
                     FVAHRLGHIDESSDQGHVLRFAAAGAVCGLVFNMIFDPLFGYYYKLLILGKPAAELAL
                     VWNVASTSINAVLSAIASLVIYMPLRRALIRSGLVDKLK"
     gene            441..1286
                     /locus_tag="ACZPCG_11680"
     CDS             441..1286
                     /locus_tag="ACZPCG_11680"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HIR1285745.1"
                     /translation="MPNYHQKKIAMINDLSGYGRCSLTVALPILSAMGIQCCPVPTSI
                     LSNHTGFPVYYFDDYTDHMEPYIEKWKELGLTFDGIATGFLGSERQIEIVKDMIAAFR
                     TPGTKVLVDPIMGDHGIAYATYTPSMCSRMRELAAMADILTPNLTEACILADVPYESG
                     EMSYEALAGLVRRLQKLGPSAVVITGIRKGPDLVNAMAEGEGEVSFLSRKAAGEERPG
                     TGDVFSSIIAGGCIRGWSLKRSVNLAADFVRDCIVRSGELQIPVKNGVCFELLLGSLC
                     QMTDK"
     gene            1393..2682
                     /gene="serS"
                     /locus_tag="ACZPCG_11685"
     CDS             1393..2682
                     /gene="serS"
                     /locus_tag="ACZPCG_11685"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HIR1285746.1"
                     /translation="MLDLKFVRENPDIVKQNIKNKFQDGKLPLVDEVIALDAENRATQ
                     KEADDLRASRNKLSKQIGALMGQGKKEEAEEVKKQVSANSARLAELEAKESELAEKIL
                     KIMMTIPNIIDPSVPIGKDDSENVEIEKFGEPVVPDFEIPYHTDIMERFNGIDLDAAR
                     RVAGNGFYYLMGDIARLHSAVISYARDFMIGRGFTYCVPPFMIRSNVVTGVMSFAEMD
                     AMMYKIEGEDLYLIGTSEHSMIGKFIDTITPEAELPMTLTSYSPCFRKEKGAHGIEER
                     GVYRIHQFEKQEMIVVCRPEESPVWYDKLWQNTVDLFRSLDIPVRTLECCSGDLADLK
                     VKSCDVEAWSPRQKKYFEVGSCSNLGDAQARRLKIRVNGEDGTKYLAHTLNNTVVAPP
                     RMLIAFLENNLQADGSVKIPEALRPYMGGMEVMIPQK"
     gene            2697..3065
                     /locus_tag="ACZPCG_11690"
     CDS             2697..3065
                     /locus_tag="ACZPCG_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR1285747.1"
                     /translation="MVKFNHFNFNVLNLEKSLEFYEKALGLKPVREKDASDGSFKLVY
                     LGDGLSDFTLELTWLKDRTESYNLGECEFHLAFHVDDYDAIHKKHQEMGCICYENPAM
                     GIYFISDPDGYWIEIVPENK"
     gene            complement(3147..3581)
                     /locus_tag="ACZPCG_11695"
     CDS             complement(3147..3581)
                     /locus_tag="ACZPCG_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HIR1285748.1"
                     /translation="MLNISGLKEGIVLDHIEAGKSLDIYYHLGLDKLDCQVAIIKNAR
                     SNKMGRKDIIKIEGGLDKLDLDILGYIDHNITVNIIRDDRIAEKKALKLPKKITNVIH
                     CKNPRCITSIEQELPHIFYLADEKTETYRCQYCEEKYSEYNQ"
     gene            complement(3575..4498)
                     /gene="pyrB"
                     /locus_tag="ACZPCG_11700"
     CDS             complement(3575..4498)
                     /gene="pyrB"
                     /locus_tag="ACZPCG_11700"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HIR1285749.1"
                     /translation="MRHLLNPLDFSVEEIDDLLALARDIEKNLPRYAHVCDGKKLATL
                     FYEPSTRTRLSFEAAMMNLGGNVLGFSSANSSSAAKGESVADTIRMISCYADICAMRH
                     PKEGSAFVAAQKAEIPVINAGDGGHQHPTQTLTDLMTIRSLKGRLDNLTIGLCGDLKF
                     GRTVHSLISAMVRYPGVKFVLISPPELRIPDSIREDVLEANNIPFEEVGNLDDALGSL
                     DILYMTRVQKERFFNEEDYIRLKDCYILTRKKMKLAKPDMYILHPLPRVNEISVEVDD
                     DPRAAYFKQAQYGVYVRMALIMTLLEVEKPC"
     gene            4779..>5308
                     /locus_tag="ACZPCG_11705"
     CDS             4779..>5308
                     /locus_tag="ACZPCG_11705"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009262446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1285750.1"
                     /translation="MTETEKKAPIPVIRVRNLYKIYRVGETKVRALNGVDFEIYKGEF
                     VAIVGTSGSGKSTLLNMLAGLEKPTKGEIIIGKVHMERLTEKQLVAFRREKVGFIFQS
                     YNLLNTMNAVENVALPLSFRGVAKRVRLKKAREFLALMGVADQEKHMPNQMSGGQQQR
                     VGIARALVVDPQIIFA"
BASE COUNT         1374 a         1169 c         1407 g         1358 t
ORIGIN      
        1 tgatcctggg cggcttttac ggcggtctgg gcggagccat cggcatgacc attggggatc
       61 ttttcgatcc tgtctatgta atatatgccc ctaagacctt tatcctgaag ctctgtatcg
      121 gtctgatcac cgggtttgtg gctcacaggc tggggcatat cgatgaaagc agcgaccagg
      181 gccatgtgct gcgctttgca gcagcaggag cggtctgcgg tcttgtcttt aatatgatct
      241 ttgatccgct gtttggatat tattacaagc ttctgatcct tggaaagccg gcggcagagc
      301 tcgcccttgt atggaacgtg gcctccacct ccatcaatgc agttctttcc gccattgctt
      361 cccttgtgat ctacatgccc cttcgccggg ctttgatccg atccgggttg gttgacaagc
      421 tgaaataagg agtatccgtt atgccaaatt accatcagaa gaaaattgca atgataaacg
      481 atctttccgg gtacggccgc tgttctctta cagtggctct tcccatttta tcggccatgg
      541 gcatccagtg ctgtccggtg ccaacctcga tcctttccaa tcacacaggc tttccagtgt
      601 attattttga tgattatacc gaccatatgg agccctatat tgagaaatgg aaggagctgg
      661 ggcttacctt tgacggcatt gccacaggat ttctgggctc ggagcgccag attgagattg
      721 tgaaggatat gatcgcagct tttcgcacgc ctgggacaaa ggttctggta gatcccatta
      781 tgggagatca cggaatcgcc tatgcgacct acacgccgtc catgtgcagc cggatgcggg
      841 agctggctgc catggccgac atattgactc ccaaccttac agaggcctgt attctcgccg
      901 atgttcccta cgagagcggg gaaatgagtt atgaggcgct ggccggcctt gtaagacgtc
      961 ttcagaagct ggggccttcc gctgttgtga tcacagggat ccgaaaggga cctgacctgg
     1021 taaatgccat ggcagaggga gaaggtgagg tttccttttt gagccgcaaa gccgcaggag
     1081 aggaacggcc cggtacggga gatgtgtttt cctccattat cgccggggga tgcatccggg
     1141 gctggagcct aaagaggtct gttaatctgg cggctgattt cgtaagagac tgcattgtgc
     1201 gttccggaga gcttcagata ccggttaaaa acggagtctg ttttgaactg cttttaggca
     1261 gcctgtgcca gatgacggac aaataggcgc cggaagggag ctttttcaaa aaaaggcttt
     1321 tatttcactg gaaaatgtta tatactcata aacaagtatg taaatacagt aaggagatcc
     1381 aggaggagta tcatgttaga tttaaagttt gtcagagaaa accctgacat cgtaaagcag
     1441 aatattaaaa ataaatttca ggacggaaaa cttcccctgg tagacgaggt gatcgcgctg
     1501 gacgctgaaa accgcgctac ccagaaggaa gctgacgatc tcagagccag cagaaacaag
     1561 ctgtccaagc agatcggcgc tctcatggga cagggaaaga aggaagaggc agaagaggtg
     1621 aaaaaacagg tcagcgccaa ttccgccaga ctggctgagt tagaggcaaa ggagagcgag
     1681 cttgccgaga agatcttaaa gatcatgatg accatcccaa atatcatcga tccaagcgta
     1741 cccatcggta aggatgacag tgaaaatgta gagatcgaga agttcggtga gccagtggtt
     1801 cctgattttg agatccccta ccatacagat atcatggagc gttttaacgg cattgatctg
     1861 gatgctgcca gaagagtagc aggaaacggc ttctactatc tgatgggcga tattgccaga
     1921 cttcactctg cagtgatttc ctatgcaaga gacttcatga tcggacgcgg ctttacctac
     1981 tgtgtacctc cctttatgat ccgcagcaat gtagtaacag gcgttatgag ctttgccgag
     2041 atggatgcca tgatgtataa gatcgagggc gaggatctct atctgatcgg taccagcgag
     2101 cattccatga tcggaaagtt tattgacacc attacgccgg aggcagagct tcccatgacg
     2161 ctcaccagct attccccatg cttccgcaag gagaagggtg ctcacggcat tgaagagaga
     2221 ggtgtatacc gcatccacca gtttgagaaa caggagatga tcgttgtctg cagaccggag
     2281 gaaagccctg tatggtatga caagctgtgg cagaataccg tagatctgtt ccgttccctg
     2341 gatatcccgg taagaacctt agagtgctgc tccggcgatc tggctgatct taaggtgaag
     2401 tcctgtgatg tagaggcatg gtccccacgg cagaagaaat attttgaggt gggaagctgt
     2461 tccaacttag gagatgctca ggcccgccgc ttaaagatcc gtgtaaatgg cgaagacgga
     2521 accaaatatc tggcacatac tttaaacaac accgtagtag cgcctccccg tatgctgatc
     2581 gctttcctgg agaacaatct gcaggcagac ggcagcgtaa agatccctga ggcattaagg
     2641 ccttatatgg gcggaatgga agttatgatt cctcagaaat aaacaggagt aaatatatgg
     2701 taaaattcaa tcattttaat tttaatgtac tgaacctgga aaagagcctg gagttttatg
     2761 agaaagccct gggacttaag ccggtaagag aaaaggatgc ttctgacggc tcctttaagc
     2821 tggtgtattt aggagacggt ctcagcgact ttactctgga gctgacctgg cttaaggacc
     2881 gcacagaatc ctacaatctg ggtgagtgcg aattccatct ggctttccat gtggatgatt
     2941 atgacgcgat ccataaaaag caccaggaaa tgggctgcat ctgctatgaa aatccggcta
     3001 tgggaattta tttcatttcc gatccggatg gatactggat tgagattgtt ccggaaaaca
     3061 aataaagaag gaaaaaggaa accggcctcc gctttaaagc ggggagccgg ttttttaacg
     3121 ggctgcgtcc aactggacgg agcgtattac tgattgtact cgctgtattt ctcctcacag
     3181 tactggcagc ggtaggtttc tgtcttttca tctgccagat agaagatgtg tggaagctcc
     3241 tgttcgatgg aggtaatgca gcgcggattc ttgcagtgga tcacgttggt gatctttttg
     3301 ggaagcttta aggccttctt ttcagcaatt ctgtcatcac ggatgatatt tacggtgatg
     3361 ttgtgatcga tatagcccag aatatccaga tccagcttat ctaaaccgcc ttcgatcttg
     3421 ataatgtcct tgcggcccat tttgttgctt cgggcatttt taatgatggc aacctggcag
     3481 tctaatttgt caaggcccag gtgatagtaa atatccaggc tctttcctgc ctcaatgtgg
     3541 tcaaggacaa tgccttcttt taatccgctg atatttaaca tggcttttct acctccagta
     3601 atgtcatgat cagtgccata cggacatata cgccgtactg ggcctgtttg aagtaagcag
     3661 ctcttggatc gtcatctacc tccacagaga tttcgtttac tcttggaagg ggatgaagga
     3721 tgtacatatc cggctttgcc agcttcatct ttttgcgtgt caggatatag cagtccttta
     3781 agcggatgta atcctcttca ttgaagaagc gctccttctg cacgcgggtc atataaagga
     3841 tatccaggct tcccagagcg tcgtccagat ttcctacctc ttcaaatggg atgttgttgg
     3901 cttccagtac gtcctcgcgg atgctgtctg gaatacgaag ctccggcggg gagatcagaa
     3961 cgaattttac acccggatag cggaccatgg cgctgatcaa agaatgaaca gtacggccga
     4021 atttaaggtc gccgcacagg ccgatggtta aattgtccag gcgtcccttt aaggagcgga
     4081 tggtcataag gtctgtcaga gtctgggtag gatgctgatg gccgccgtca cctgcattga
     4141 tgacaggaat ttctgccttc tgggctgcta caaatgcgga accctccttg ggatgacgca
     4201 tggcgcagat atcagcgtag caggaaatca tacggatggt gtcggcaacg ctttctccct
     4261 ttgcagcgga actggaatta gcggaagaaa agccgagaac attgccgccc aggttcatca
     4321 tggctgcttc aaagcttaag cgtgttctgg tgctgggctc ataaaacagg gtagcaagct
     4381 ttttgccgtc gcaaacgtgc gcatatctgg gaagattttt ctcgatgtct ctggctaatg
     4441 ctaaaaggtc atcgatttcc tccacagaga agtctaaggg attcagtaag tgtctcatgt
     4501 gattctcctt tgccttttct attttatttt aagtgatttt gggaaatagt ccacactgaa
     4561 ccagtgcgat agccggacag ccagatatgg cactgatttg tgcctcgtca tattcttaca
     4621 tagaatgaca aagaagtcaa gtagaatttg ggcggccgta aaaaatattt aaggaacttt
     4681 aaagaaaaaa gggacagcgg ctattgacgt tttatggtat actgtctaaa gacagacaac
     4741 acaaccatgc cttttacagg caggcaaagg agagttcatt gacagaaaca gagaagaaag
     4801 ctccgattcc cgtaatccgt gtaaggaatc tgtataagat ttaccgggtg ggagagacaa
     4861 aggtacgggc attaaacggc gtggattttg aaatctataa gggagagttt gtagccattg
     4921 taggaacctc cggttccggc aaatccaccc ttttgaatat gctagccgga ctggagaagc
     4981 ccaccaaagg cgagatcatc ataggaaagg ttcatatgga acggctgacg gaaaaacagc
     5041 ttgtggcatt caggagggaa aaggttggat ttattttcca gtcctataat cttttaaata
     5101 ccatgaatgc cgttgaaaat gtagcccttc ccttatcgtt ccggggagtg gccaaaagag
     5161 tgcggcttaa aaaggccagg gaatttctgg cgctgatggg ggtggcagat caggaaaagc
     5221 atatgccaaa ccagatgtcc ggaggccagc agcagagagt gggcatcgcc agggctctgg
     5281 tggtagaccc gcagatcatt tttgcgga
//
LOCUS       DBMDUH010000172         5229 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_6085_length_5229_cov_7.045827, whole genome shotgun sequence.
ACCESSION   DBMDUH010000172 DBMDUH010000000
VERSION     DBMDUH010000172.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 5229)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5229)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5229
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6085_length_5229_cov_7.045827"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="ACZPCG_11710"
     CDS             <1..203
                     /locus_tag="ACZPCG_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HIR1285751.1"
                     /translation="PHIEGYEKILQAAMECNFEKAYEAFMKDPLVHGRISFGDGEKLL
                     KEMIGNTRKYLPEEWEKVFLKK"
     gene            401..1225
                     /locus_tag="ACZPCG_11715"
     CDS             401..1225
                     /locus_tag="ACZPCG_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HIR1285752.1"
                     /translation="MIEQKRLQSHPPICTVPEEQKEKIMEIAMRAGHILLENGAEISR
                     VEETIDRICRHYGIESANSFVLSNGIFSTMGNERESFFARVQHIPVSGARLNRVAAVN
                     QLSREIEEGKHTVEELGRCLDDIEQMPGKRKGVQIGASAVGSGAFCYLLGGDLADSAA
                     AFLSGLVLYVYILYIGKNLSKLVQNILGGALVTFCCVVLYHLGLGHHISSMIIGSVIP
                     MIPGVAFTNGIRDITDGDYIAGSVRMMDAGLVVLSIALGMGFVLTMYYRLTGGNLL"
     gene            1222..1683
                     /locus_tag="ACZPCG_11720"
     CDS             1222..1683
                     /locus_tag="ACZPCG_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007716888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HIR1285753.1"
                     /translation="MNAFPLGELAGEMAAAAVGTAAFSVLFSVPKKYYGLCAACGTIG
                     WLVYFCFHWDGLSPAESTFVSAAAVIFLSRLFAVWERCPATLFMIPGIFPIVPGAGIY
                     WTAHYIVTDQLALASRTGFEAVKAAAAIVLGIIFVFEIPQRFFGRIAKRKE"
     gene            1686..2417
                     /locus_tag="ACZPCG_11725"
     CDS             1686..2417
                     /locus_tag="ACZPCG_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HIR1285754.1"
                     /translation="MIEICCGSYEDALAAWKGGAERIELNSAVYLGGLTPSIGSLRLV
                     KENTGLKVISMVRPRGAGFSYTEAETEQMFADARILMENGSDGLAFGFLTSEGKIDSE
                     RTGRMIRLIHEFHGEAVFHRAFDCVEDPYEAIEELIRLGTDRILTSGLKEKAAAGAGL
                     LKELQENYGNQIQLLAGSGINADNAKALLEETGLTQVHSSCRDWKRDETTTGPWVNYC
                     FAPVPHENDYDAVNETLVRKLKGSV"
     gene            2414..3280
                     /locus_tag="ACZPCG_11730"
     CDS             2414..3280
                     /locus_tag="ACZPCG_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003525438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIR1285755.1"
                     /translation="MMKKNSKTLRYLMTAAAVTLSALLQAFAMNVFLTPTGILPSGFT
                     GVARLTEQITGLLGIHFSTSLGMIALNVPVAIFCYRHIGKKFVIFSMIQVFLASFLLK
                     LSLFEPLFEDALLNICFGGFIYGLAIVVALKGNASTAGTDFIALYVSNRLGKAIWQYV
                     FAFNAGMLCVFGYLFGWKYAGYSIMFQFISTKTIDSFYHRYKRVTLQVTTEEPEAVVK
                     AYVGQYRHGISVLDGHGGYSGKKMSLLHTVVSAYEVQDIVHLMRQVDPHVIVNVLPTE
                     TFFGGFYQKPIE"
     gene            3402..4310
                     /gene="rfbA"
                     /locus_tag="ACZPCG_11735"
     CDS             3402..4310
                     /gene="rfbA"
                     /locus_tag="ACZPCG_11735"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HIR1285756.1"
                     /translation="MKGIILAGGSGTRLYPLTKVTSKQLLPIYDKPMIYYPLSVLMNA
                     GIRDILIISTPEDTPRFKALLGDGHPFGINLSYAVQPSPDGLAQAFIIGEEFIGGDSV
                     AMVLGDNIFHGQGLTKRLKAAAAREKGATVFGYYVDDPERFGIVEFDEKGKAISIEEK
                     PEKPKSNYCVTGLYFYDNRVVEYAKNLKPSARGELEITDLNRIYLENGELDVILLGQG
                     FTWLDTGTHESLVEATNFVKTVETHQHRKIACLEEIAYLNGWITKEQVLETYEVLKKN
                     QYGKYLKDVLDGKYVDKLKEQQSLEE"
     gene            4316..>5229
                     /gene="rfbB"
                     /locus_tag="ACZPCG_11740"
     CDS             4316..>5229
                     /gene="rfbB"
                     /locus_tag="ACZPCG_11740"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HIR1285757.1"
                     /translation="MKIIVTGGAGFIGSNFVHHMVNKYPDYEIINLDLLTYAGNLENL
                     KPVENKPNYKFVKGDIADRAFIFDLFEKEKPDMVVNFAAESHVDRSITDPEAFVRTNV
                     MGTTTLLDACRTYGIKRYHQVSTDEVYGDLPLDRPDLFFTETTPLHTSSPYSSSKASA
                     DLFVLAYHRTYGLPVTVSRCSNNYGPYHFPEKLIPLIISRALANEELPVYGTGENVRD
                     WLHVADHCEAIDLVIHKGREGEVYNIGGHNERTNLEVVKTILKALDKPESLIRFVTDR
                     PGHDMRYAIDPTKIETELGWKPKYNFDTG"
BASE COUNT         1366 a         1112 c         1407 g         1344 t
ORIGIN      
        1 tgcctcatat tgaaggttat gaaaaaattt tgcaagctgc aatggaatgt aactttgaaa
       61 aagcctatga agcttttatg aaagatcctt tggttcatgg gcgtatcagt tttggagatg
      121 gagaaaaact tctgaaagaa atgattggta ataccagaaa atatcttcct gaagagtggg
      181 aaaaagtatt tcttaaaaaa tgatatgaag taaacaaaaa ggagtatgat tcagttcgta
      241 ctcctttaat cattatattg tggtatatgt tttctttaaa tgcagcatga aaaaggataa
      301 aagtaatatt cggatattcg gattgcaagg aatggttgtt ttcatgggga attcagagta
      361 caataaaaat atgtgtttaa taagcaggag ataatataaa atgatcgaac agaaacgact
      421 acagtcccat ccccccatct gcaccgtccc tgaagaacag aaagaaaaaa tcatggaaat
      481 cgccatgcgt gccgggcata tccttttgga aaatggggcg gagatttcac gagtggagga
      541 aactattgac cggatctgcc gtcattacgg gattgaatcc gccaattcct ttgtactgag
      601 taacggtatt ttttctacca tggggaacga aagggaaagc ttttttgcca gggtacagca
      661 cattcctgtc agcggggcgc gcttaaaccg tgtggcggct gtaaatcagc tttccaggga
      721 gattgaagag ggaaagcata cggtagagga gcttgggaga tgcctggatg atattgaaca
      781 gatgcccgga aagagaaagg gcgttcagat cggggcttcg gcggtgggaa gcggggcgtt
      841 ctgctatctg ctgggcggtg atctggcaga cagcgcagct gcttttctgt ccggccttgt
      901 gctgtatgtc tatattttat atatagggaa aaatctgtca aaactggtgc agaatattct
      961 gggaggagct ctggtgacct tctgctgcgt ggtgctttac catctggggc tgggccatca
     1021 catcagctcc atgatcatcg gttctgtgat ccccatgatt ccgggagtgg cgtttacaaa
     1081 cgggatccgc gatattacgg atggagacta cattgccggt tctgtgcgga tgatggatgc
     1141 agggctggtg gttttaagca ttgccctggg aatgggattt gtacttacca tgtactacag
     1201 attgacagga ggaaatcttt tatgaatgcg tttccattgg gagaactggc aggggaaatg
     1261 gctgccgccg cagtaggaac ggctgctttt tcagtcctct tttctgtgcc gaaaaaatat
     1321 tacgggcttt gtgctgcctg tggtaccatc ggctggctgg tgtatttctg ttttcactgg
     1381 gacggacttt cgcccgcaga gtctacgttc gtttccgcag ccgccgtaat ctttctgtcc
     1441 aggctctttg cggtctggga gcgctgtccg gctaccctgt ttatgattcc aggtattttc
     1501 cctattgttc ccggagccgg catctactgg accgcccact acattgtcac cgaccagctg
     1561 gcgctggcaa gccgcacggg atttgaggcc gtgaaggcag cggcagccat tgtgctggga
     1621 attatatttg tatttgaaat cccccagagg ttttttggga ggattgcaaa gagaaaggag
     1681 tagttatgat agaaatatgc tgcggaagct atgaggatgc cctggcggcc tggaagggag
     1741 gcgcagagcg gatcgaatta aacagtgcgg tgtatctggg aggcctgacg ccttccatcg
     1801 gaagtctgcg gctggtaaag gaaaatacgg gattgaaggt gatttccatg gtacggccca
     1861 gaggagcggg atttagctac acagaagcgg aaacagagca gatgtttgca gacgcccgta
     1921 ttttgatgga aaatggaagc gacgggcttg cttttggctt tttgacatct gagggaaaaa
     1981 ttgacagtga aaggacaggc cgtatgatcc gcctgatcca tgaatttcat ggggaagccg
     2041 tattccacag ggcttttgac tgtgtggagg atccatatga agccattgag gagctgatcc
     2101 gcctgggaac agaccgtatt ctcaccagcg ggcttaagga aaaggcagct gccggagccg
     2161 gtcttttaaa ggagcttcag gaaaactatg gaaaccagat ccagcttctg gcaggaagcg
     2221 gcatcaacgc agataatgca aaagcgctgt tggaggaaac cggccttacc caggttcatt
     2281 cctcctgcag ggactggaag cgggatgaaa ctaccacagg cccttgggtg aattactgct
     2341 ttgctccggt gcctcatgaa aatgattacg atgcagtaaa tgaaacgctg gtaagaaagc
     2401 tgaaagggag tgtatgatga aaaagaacag taaaacgctg aggtatctga tgacagcggc
     2461 ggccgtaact ctttccgctc ttttgcaggc ctttgctatg aatgtttttt taacgcctac
     2521 ggggattctt cccagcggct ttacgggagt ggcccgtctg acggagcaga ttacaggcct
     2581 gttggggatt catttttcta cttccctggg aatgatcgct ttaaatgtgc cggttgccat
     2641 tttctgttac cgccacattg gaaagaaatt tgtgattttc tctatgatcc aggttttttt
     2701 ggccagcttc cttttgaaac tgtccctgtt tgagcctttg tttgaggatg cgcttttaaa
     2761 tatctgcttt ggaggcttta tctacggtct ggctattgtg gttgcattaa aaggaaacgc
     2821 ttctaccgca gggacggact ttatcgccct ttatgtgtca aaccgtctgg gaaaggccat
     2881 ctggcagtat gtatttgcct ttaacgcagg aatgctttgt gtgttcggtt atctctttgg
     2941 ctggaaatac gcgggctatt ccattatgtt tcagttcatt tccacaaaga ccattgacag
     3001 cttttatcat cggtataagc gggtaacgct tcaggtgaca acggaggagc cggaagcggt
     3061 ggtaaaggcg tatgtaggtc agtaccgtca cggaatctct gtcctggacg gccacggagg
     3121 ctacagcgga aagaagatga gcctgctcca caccgttgtc tccgcgtatg aggttcagga
     3181 tattgtccac ctgatgcgcc aggtggatcc ccatgtaatt gtaaatgttc tgcccacaga
     3241 aacatttttc ggcggctttt atcagaaacc cattgaataa aaatccttaa tattcccaaa
     3301 atattgcttc cagtatttga aaatcagggg attgtatggt atagtgggga aagaaataat
     3361 ggtcggatcc gctgcgcggc ggatctgcaa ggaggaaata tatgaaggga atcatacttg
     3421 ccggaggaag cggaaccaga ctgtatccgc tgacaaaggt tacatccaag cagcttctgc
     3481 ctatttacga taaacccatg atctattacc cgctgtctgt gctgatgaat gcaggaatcc
     3541 gggatatact gatcatttcc acaccggagg acacgccccg gtttaaagcg cttttgggag
     3601 acggacatcc ctttggcatc aatttaagct atgcggttca gccaagcccc gacggactgg
     3661 ctcaggcctt tatcatcggg gaggaattta tcggcggcga ttccgttgcc atggttttag
     3721 gcgataatat tttccatgga cagggtctta caaagcggtt aaaggctgcg gctgccaggg
     3781 aaaagggcgc aaccgtgttc ggctactatg tggacgatcc ggagcgtttc ggcattgtgg
     3841 agtttgatga aaagggaaag gctatttcca tcgaagaaaa gccggagaag ccaaagtcaa
     3901 actactgtgt cacaggactt tatttctacg acaatcgtgt ggtagagtac gcaaagaact
     3961 taaagccttc tgccagagga gagctggaga tcacagactt aaaccgcatc tatctggaaa
     4021 acggcgagct ggatgtgatc cttttgggac agggctttac ctggctggat acgggaaccc
     4081 atgagtcttt agttgaagct accaatttcg taaagaccgt agaaacccac cagcacagaa
     4141 agattgcctg cctggaggaa atcgcctact taaacggctg gatcacgaag gagcaggttc
     4201 ttgaaaccta tgaagtgctt aagaaaaatc agtacggaaa gtatttaaag gatgttctgg
     4261 acggcaaata tgtggataag ctgaaggaac agcagagttt ggaggaatag aatcaatgaa
     4321 gatcatcgtt accggcggag ccggatttat cggaagtaac tttgtacacc atatggtaaa
     4381 taaatacccg gattatgaga tcatcaatct agatcttctg acctatgcag gcaatctgga
     4441 gaatttaaag ccggttgaaa ataagcccaa ctacaagttc gtaaaaggcg atattgcaga
     4501 cagagcattt atcttcgatc tgtttgagaa ggaaaaaccg gatatggtgg tgaactttgc
     4561 ggctgagagc catgtagacc gttctattac tgatccggaa gcctttgtgc gtaccaatgt
     4621 aatgggaact actacactgc tggatgcctg ccgtacctac ggtatcaagc gctatcatca
     4681 ggtgtcaacg gatgaggtgt acggagatct gccattagat cgtccggatc tcttctttac
     4741 agagacgacc cctcttcaca catccagccc ttattcttcc tccaaggcaa gcgcagacct
     4801 tttcgtgctg gcttaccaca ggacctacgg gctgcctgtg accgtatccc gctgctccaa
     4861 caactacggt ccctatcatt tcccggagaa gctgatcccc ctgatcatca gccgtgctct
     4921 ggcaaatgag gagcttcccg tatacggaac cggtgaaaat gtccgtgact ggctccatgt
     4981 ggcagaccac tgcgaggcca ttgatctggt gatccacaag ggacgcgagg gcgaggttta
     5041 caatatcggc ggacacaacg agcgcaccaa tctggaggtt gtgaagacca tcttaaaggc
     5101 cctggacaag ccggaaagcc tgatccgctt cgttacagac cgtccgggtc acgatatgcg
     5161 ctatgccatt gaccccacca agatcgagac agagcttggc tggaagccaa agtataactt
     5221 tgacacagg
//
LOCUS       DBMDUH010000173         5123 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_6229_length_5123_cov_4.514337, whole genome shotgun sequence.
ACCESSION   DBMDUH010000173 DBMDUH010000000
VERSION     DBMDUH010000173.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 5123)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5123)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5123
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6229_length_5123_cov_4.514337"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            40..549
                     /locus_tag="ACZPCG_11745"
     CDS             40..549
                     /locus_tag="ACZPCG_11745"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0141098 - tRNA
                     (cytidine(34)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HIR1285758.1"
                     /translation="MMNIVLLEPEMPSNTGNIGRTCVATNTRLHLIEPLGFKLNEKAL
                     KRAGLDYWDKLDVSIYSDYQDFLEKNPQAAGNMYFATTKAHKVYSDVIYSPDCYLMFG
                     KESAGIPEEILVENEEHCIRIPMWGDIRSLNLSNSAAIVLYEALRQNGFEKMELAGEL
                     HHLSWNSQI"
     gene            658..1917
                     /locus_tag="ACZPCG_11750"
     CDS             658..1917
                     /locus_tag="ACZPCG_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HIR1285759.1"
                     /translation="MSGLQKWLEKWLMPIARVLEKQKHLQAVKDGIVGIVPIIIIGSF
                     CLVPTGIGNLVGGGLMEWVNAHNNILTFPTYFTTNVMSLYAALYIANSLAKRYKLKNS
                     LIGASAILVHLLLSVTVTEDGTWMVTYLAAEGLFTAIISAIFAVEITRFCDKNHLTIK
                     MPESVPPMVADSFSILVPLIIEVLVATGVAALCQNIAGMVFPQVIMKVLAPAIASMDS
                     LPMVMLVVFLTQLLWMFGLHGPAITSSVWAPFAIANATANAAAVAAGQDPTHVFTFGF
                     YYGFLQVSGSGITLGLVLLMMFSKAKSLKTIGRVGIIPSLFGINEPIIFGTPIIMNPF
                     LMIPFVLGPVLIAAVNYLAMSTGLVGLPLWEAPGFLPPGFQAFLLTLDWKAAVMSVLN
                     VVVMTVIYYPFFKMMEADELKKEMAGK"
     gene            2003..3946
                     /locus_tag="ACZPCG_11755"
     CDS             2003..3946
                     /locus_tag="ACZPCG_11755"
                     /inference="COORDINATES: protein motif:HMM:NF019884.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HIR1285760.1"
                     /translation="MLSIRQKTIVRYILGHPGGVAGHQLSELLRVSGKTIRNDISDIN
                     RWMKEYEIRICASQKEGYYIPEAYRNHMMSVLENQADPDIKWEIHTPQERRLAIMGRV
                     LGHPGIRLDQIADRFCVSEQTLYKDFIHLKNTLSETCGFNQFVMKSHCLYVQAEEYDI
                     LRLIFRLISSCIMSSGQMMDSLLMHWMQGIVNLGEIYTFYGYTVQYCREKKLIIPDPV
                     LYISSWLIFYVNMRREEAHFLEKEDRSLPEDELSGFLQYMDKSLFLELDSCDFAFLYD
                     YLKAIGFPAEGGMEDQEAEELAACFQEKLWKQNQIKLSEREETADLYRSFVGDLKGLI
                     LRIHLEAQLFEFTSKAEQDTLREAFGAMLLMGVLIREKYGVDVTPAELRRLSGYIEAG
                     CPSEQLRVSIQMVTGSDSSFYYSVSRWIRNNFSENVRICGVCPGYLLESSIEKNEPDI
                     LMAVTPLNIQSSIPQITLTIPFSVEEGNRLWRFIEDLSLKKQTEFVLESFFNHDRVVF
                     LEGNPSWEEMAGAGCRNLENTGCVRAWKEHLREFSEKEAWSKSPVANGFCFLYPREKK
                     DLKDGISLVIARNRRSDIRVMMAAAFAPGNYGKQEALYGFFRRFWKEQFLAETLQKEK
                     TASDVLERIRSSVQDEMKYKIKI"
     gene            3963..4259
                     /locus_tag="ACZPCG_11760"
     CDS             3963..4259
                     /locus_tag="ACZPCG_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HIR1285761.1"
                     /translation="MRVLVVCNAGMSSSILVKKMKDYAASQGEELMIKAVSSAAAENE
                     AGEWDVCLVGPQLMHAVDEIAEAVEVPTEAVDMRTYAMADGEGALKQAKALLEK"
     gene            4275..5012
                     /gene="deoD"
                     /locus_tag="ACZPCG_11765"
     CDS             4275..5012
                     /gene="deoD"
                     /locus_tag="ACZPCG_11765"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016297137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HIR1285762.1"
                     /translation="MATIHNEAEPEQIAPVVLMPGDPLRAKLIAEEYLENCILFNQVR
                     NMLGYTGWYKGHRISVMGGGMGCPSTGIYAHELFCKYNVDSIIRVGSCGAFSEEVAMR
                     DLLLIRSSWSESSFARAYSGFEGDTAFPSEDLNGLLSQAAETLGQSLKQVKVHTADSF
                     YKKDKEANFKIRDQWGCVAEEMESFALFHVAEVSGKKAAALLTVSDHALTRKKISAEE
                     RQNSFRSMIETALLGAVLRLEEMEKRL"
BASE COUNT         1415 a          930 c         1328 g         1450 t
ORIGIN      
        1 gcgctttgta aagaaccatg acggaaaggc gtgacagcta tgatgaatat tgtactttta
       61 gaaccggaaa tgccttctaa tacaggcaac atcggccgca cctgcgttgc aaccaataca
      121 aggctccatc tgatcgagcc tttgggcttt aagctcaatg aaaaggcctt aaaacgggcc
      181 ggactggatt actgggataa gctggatgta agcatataca gcgattacca ggattttctc
      241 gaaaaaaatc cacaggctgc aggcaatatg tattttgcca caaccaaggc ccataaggtt
      301 tattctgacg taatctacag cccggactgt tatctgatgt tcggcaaaga aagcgccggt
      361 attcctgagg aaatcctggt ggaaaatgag gagcattgca tccgcatccc catgtggggc
      421 gatatccgct ccttaaacct ctccaattct gccgccattg tgctttatga ggccctgcgc
      481 cagaatggat ttgaaaagat ggagctggcg ggagagctgc atcatctttc ctggaatagc
      541 cagatctaat atttataaat ttaccttttc tgttaggaat ttaattattt gtgataaaat
      601 ccctcctatt ttataatcgc tcttagataa aatacacatc tgaggaggga ttccattatg
      661 agtggacttc aaaagtggct tgaaaaatgg ctgatgccta tagcacgtgt tctggagaaa
      721 caaaaacatc ttcaggccgt gaaagatggt atagtaggta ttgtgccaat cattatcatc
      781 ggctccttct gcctggttcc tacaggtatt ggcaacctgg tagggggagg actgatggaa
      841 tgggtaaatg cccacaacaa cattcttaca tttcccacat attttacaac gaacgtaatg
      901 tctttgtatg cggcgcttta cattgccaat tctcttgcaa agcgttataa gctgaagaat
      961 tcgctgattg gtgcttctgc aattctggtt catctgctgt tatctgtaac tgttacagag
     1021 gatggaacat ggatggtgac atatttggcg gcagagggct tatttactgc tattatttct
     1081 gcgatttttg ctgtagagat tactcgattc tgtgataaga atcacctgac gatcaaaatg
     1141 cccgagagtg tacctcctat ggtagcagac agcttttcta ttctggttcc actgattatt
     1201 gaggttcttg tcgctacagg tgtggcagct ttgtgccaga atatagcagg aatggtattt
     1261 ccgcaggtga ttatgaaagt gctggctcct gccattgcgt ccatggacag ccttccaatg
     1321 gtgatgctgg tggtgttcct tacacagctt ttatggatgt ttggacttca cggtcctgcg
     1381 atcacatcca gcgtgtgggc gccttttgca atcgccaatg ctacagccaa tgcagcagca
     1441 gtagctgccg gacaggatcc gacacatgta tttacctttg gtttttatta cggattttta
     1501 caggtaagtg gctctggaat tactctggga ctggtacttc ttatgatgtt ttctaaggcg
     1561 aagtccttaa aaaccattgg gcgggtagga attattcctt ctctgtttgg aatcaacgaa
     1621 ccgattattt ttggaacgcc aatcatcatg aacccatttc tgatgatacc gtttgtactt
     1681 ggccctgtac tgattgcggc agtgaattat ctggcaatgt ctacaggttt ggtgggactg
     1741 cctctgtggg aggcccccgg atttcttcct cctggttttc aggctttttt acttacgctg
     1801 gactggaaag cggccgttat gtctgtgctg aacgtagtcg ttatgacggt tatttattat
     1861 ccattcttta aaatgatgga ggcagatgag ctgaagaagg aaatggcagg aaagtgacag
     1921 aaacagcttt tcagacagac tcggatttag tagtgtgtcc tgaaaagata tgatataatt
     1981 gacagcaaag ggagggattc acttgctgtc aatacgtcag aaaacaatcg tacgttatat
     2041 tttgggccat ccgggcggtg tagcaggtca tcagttgtca gaacttctgc gtgtcagcgg
     2101 aaaaaccata cgcaatgaca tttcagatat aaaccgttgg atgaaggagt atgaaatacg
     2161 catctgtgct tcgcagaagg aagggtatta tataccagag gcatatcgga atcacatgat
     2221 gagcgttttg gaaaatcagg cggatcctga tataaaatgg gagatacaca cgcctcagga
     2281 acggcgtctt gcaattatgg gcagagttct ggggcacccc ggaatcaggc tggatcagat
     2341 tgcagatcgc ttttgcgttt cggagcagac gctatacaag gattttatac atcttaaaaa
     2401 taccttgagt gaaacctgcg gctttaacca gtttgtgatg aagagtcatt gtctgtatgt
     2461 tcaggcggag gagtatgata ttttaagact gatctttcgc ctgatctcca gctgtattat
     2521 gagcagtggt cagatgatgg attctcttct catgcattgg atgcagggga ttgtcaattt
     2581 gggtgagata tatacatttt atggatatac agttcaatac tgccgagaga aaaagctgat
     2641 aattcctgat cctgtactgt atatttcgtc ctggcttata ttttatgtaa atatgagacg
     2701 ggaggaagca cattttttgg aaaaggaaga cagaagtctt ccggaagatg aactttccgg
     2761 ttttttgcag tatatggata aaagtctgtt tttggaactg gattcatgtg actttgcctt
     2821 tctctatgat tatttgaaag cgataggctt tccggcagag ggagggatgg aggatcagga
     2881 ggcagaagaa ctggcagcct gctttcagga gaaactgtgg aagcagaatc agattaagct
     2941 ttcagaacgg gaagagactg cagatctgta tcgatcattt gtgggagatc tgaaaggttt
     3001 gattctcagg attcatctgg aagcgcaatt gtttgagttt acttcaaagg cagagcagga
     3061 tacgctgaga gaggcttttg gagcaatgct ccttatgggt gttttgatca gagaaaaata
     3121 tggcgtggat gttacaccgg cagaattgag acgtttatcc ggttatatag aagctggatg
     3181 ccccagtgaa cagctcaggg taagcattca gatggtaaca ggatctgaca gcagctttta
     3241 ttatagtgta tcacgttgga tcagaaataa tttttcagag aatgttcgca tctgtggagt
     3301 ttgtccggga tatcttttgg aatcttccat agagaaaaat gaaccggata ttttaatggc
     3361 tgtaacgccg cttaatatac agtcatcgat tccgcagatt acccttacaa ttccattttc
     3421 ggtggaggag ggtaacaggc tttggagatt tatagaggat ctttctctga agaaacagac
     3481 agagtttgtt ctggaaagtt tttttaacca tgatcgagtg gtgtttttgg agggcaatcc
     3541 ctcctgggag gaaatggctg gcgccggatg tagaaatttg gaaaatacgg ggtgtgtccg
     3601 agcgtggaag gagcatttaa gagaattttc ggaaaaagaa gcatggagta aaagtcctgt
     3661 ggcaaatggc ttttgctttt tatatccccg cgaaaaaaaa gatcttaagg atggaatcag
     3721 tctcgtgata gccagaaaca gacggtctga tatccgggtt atgatggcag cggcttttgc
     3781 tccgggaaac tatggaaagc aggaagcttt gtatggtttt ttcagaaggt tttggaaaga
     3841 acagtttctg gcagaaacgc ttcagaaaga aaaaacggcc tctgatgtac tggaaagaat
     3901 aagatcatct gttcaggatg aaatgaaata taaaataaaa atttagcagg aggaaaaaga
     3961 aaatgagggt tttagttgta tgcaatgcag gaatgtcaag ttcgattttg gtgaaaaaaa
     4021 tgaaggatta tgccgcttct cagggagaag aactgatgat caaggcagtc agcagtgcgg
     4081 cggcagagaa tgaagccggc gaatgggatg tatgtctggt aggccctcag ctgatgcatg
     4141 ctgtagacga aattgcagaa gcagtagaag ttcctacaga ggcggtggat atgcgtactt
     4201 acgcaatggc tgacggagaa ggtgctctga agcaggctaa ggcgcttttg gaaaaataac
     4261 aggaggataa atctatggcg acaattcata atgaagctga accggaacag attgcgccgg
     4321 tggttttaat gccgggtgat ccgcttaggg ccaagctgat tgcagaggaa tatctggaga
     4381 attgcatact gtttaatcag gtgagaaata tgctcggtta tacaggttgg tataagggcc
     4441 ataggatatc tgttatgggg ggcggcatgg gctgcccctc aactggtatc tatgcccatg
     4501 aacttttttg taaatacaac gtagattcga ttatacgggt aggatcatgc ggggcttttt
     4561 cagaagaggt tgccatgagg gatctgcttt tgatccgctc ctcatggagt gaatcatcgt
     4621 ttgccagagc ctacagtggt tttgaaggag atacagcctt tccttccgaa gatttaaacg
     4681 gacttttatc ccaggcagca gaaacattgg ggcagtcttt aaagcaggta aaggtacata
     4741 ctgcagacag cttttacaaa aaggataaag aagccaattt taaaatcagg gatcagtggg
     4801 gctgtgtggc ggaagagatg gagagttttg ctttatttca tgtggcagag gtaagcggaa
     4861 aaaaggctgc tgcattgctt acagtcagcg atcatgctct caccagaaag aagatatctg
     4921 cagaggaacg tcagaattcc ttccgctcta tgattgaaac agcgcttttg ggggctgtgc
     4981 ttaggttgga agagatggaa aagcgtttgt aaaataaaat tcccatacaa aaacccgaca
     5041 gtccgtctca atagccggga ctgccgggtt ttatgtaggt gctttctgct gctcaggctg
     5101 ttttatgcct gagcaggatt ata
//
LOCUS       DBMDUH010000174         5068 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_6309_length_5068_cov_7.559694, whole genome shotgun sequence.
ACCESSION   DBMDUH010000174 DBMDUH010000000
VERSION     DBMDUH010000174.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 5068)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5068)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5068
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6309_length_5068_cov_7.559694"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="ACZPCG_11770"
     CDS             <1..600
                     /locus_tag="ACZPCG_11770"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016094 - polyprenol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HIR1285763.1"
                     /translation="IIPDGNRRWAVREGMDKADGYDHGIAPGMELFRMCRELGVEEMT
                     FYGFTVDNTKRPSAQRRAFTAACVAAVEELSKEDASLLVLGNTKSSVFPREFLPYTTR
                     RSFGKGGMKINFLVNYSWEWDFGLKDGLLGEKLKTAEVSRIDLIIRWGGRRRLSGFLP
                     VQSVYSDIYVVDDYWPDFKPSHVEEALEWYSRQDVTLGG"
     gene            complement(624..1553)
                     /locus_tag="ACZPCG_11775"
     CDS             complement(624..1553)
                     /locus_tag="ACZPCG_11775"
                     /inference="COORDINATES: protein motif:HMM:NF013627.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285764.1"
                     /translation="MKKHTLQTLCTAALISLGLTFTAMGEERITDVSLIFSYDQSPEG
                     GKEVGELTAVSSSSQFTVEGSGYLKDDDTWSYGEWPVGEVELSANEGYYFPTAARSLF
                     HLSGDGVQYQSARTEDEGSTLILHVRLPRIDGAIPAPLTADWSGHTAVWDEVDGADQY
                     EIQLLKDRRTLTTLTSSAASCSFENWINNEGSYTFRVRAYNRKDRKNSGWCSDAEPLV
                     ITPEEAWFHSDGIWKEENGKHLFLYPNGAFPQMAWRQIDGKWYFFDGAGYMVSQCYVR
                     SKSSDLYHWIGTDGVWDKNGDTKQPNRAQYEVY"
     gene            complement(1656..1970)
                     /locus_tag="ACZPCG_11780"
     CDS             complement(1656..1970)
                     /locus_tag="ACZPCG_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285765.1"
                     /translation="MDINQLMNSLKETYGFSKTEWNELNGEPLAEFSLWLTEEHISRF
                     IEKAGRLTAIIESCANMVSICEPELKDTLMATTIQCCGANQLKIRTSESMLKMLVGSI
                     FD"
     gene            complement(2070..2672)
                     /locus_tag="ACZPCG_11785"
     CDS             complement(2070..2672)
                     /locus_tag="ACZPCG_11785"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HIR1285766.1"
                     /translation="MSGKIITIARQFGSGGREAAMRTAKELNIPFYDRELVEMAAEKM
                     GHASVSVEKVDESALMGFLADYHVPREPNSVTGYGLSLPDSTYVTQTIIIETLARKGP
                     CVIVGRCGNYALRSRNDVIDLFICANMEDRIHRIMERYHFSEKDAINAIHSTDKRRKH
                     YYENYTHQKWGSIDSHQMLLNVSRLGMDRTVEIIKQLYNH"
     gene            complement(2784..3209)
                     /locus_tag="ACZPCG_11790"
     CDS             complement(2784..3209)
                     /locus_tag="ACZPCG_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285767.1"
                     /translation="MRELAAFYCPKCGYYAYYQTSRHPQCPRCNSKDTMNMVRMHYKE
                     FMNMSCQERDDYLGNEILKQNPSLMERITAPHKKYNSREVIAEMNMEIMRLDTENKIL
                     SDTVKWMHDTIWEMMREQRKAQKQADASCKKPDAHDNHT"
     gene            complement(3518..4834)
                     /locus_tag="ACZPCG_11795"
     CDS             complement(3518..4834)
                     /locus_tag="ACZPCG_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006773940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HIR1285768.1"
                     /translation="MKKQTKLLAVLSTAALAAALSFPAYAKNAGWAQENGNWYYYDSY
                     GEPLTDTWKKSGDDWYYLDSDGIRATSTQIDEYYVDEDGKRVTMKWISVENEDYWSEQ
                     DEPEFLYYYYGRDGRALTSTWASINGQWYYFNEDSIMETGSIQVDGFNYYLGEDGSRK
                     TGWVLLEDETDDPEIIESWYYFDNSGKRIEHAVDKKINGNYYTFVDGKMQTGWFKLPA
                     AENAATASEAVKADASSISGYQYYDEDGKRASGWRTIEGAEGISEEAEVYKFYFKNGV
                     PHHAQSGLELFSIDSKKYAFNTKGEMQTGKQSVNIGDGASANFYFGEDGAMKTGKQTI
                     YDEDLGETQNWYFHTDGSKKGQGYHGIKDNTLYIHGLRQEADPDLRLVPADLDGTRYL
                     INTNGAIQKAGSSSKSNAKPELGAGYKDFKDENDTIWTVNTSGIIQ"
BASE COUNT         1200 a         1164 c         1287 g         1417 t
ORIGIN      
        1 attattcccg acggcaatcg ccgctgggcg gtgagagagg gaatggataa ggcagatggc
       61 tacgatcacg gcatcgctcc gggaatggag ctgtttcgga tgtgccggga gctgggggtg
      121 gaggaaatga cgttttacgg ctttaccgtg gacaatacaa aacgcccctc tgcccagcgc
      181 cgtgcattta cagccgcctg cgtggcggca gtagaggagc tctcaaagga ggatgcaagt
      241 cttttagtgc tgggaaatac gaagtcatct gtatttccca gagaatttct gccctataca
      301 acccgccgca gcttcggaaa gggaggtatg aagatcaatt tccttgtaaa ttacagctgg
      361 gaatgggatt tcggcttaaa ggacgggctg ctgggagaga agctgaaaac cgctgaagtg
      421 tcccggatcg acctgattat acgctggggc ggcaggcggc ggctgtcagg ctttctccct
      481 gtgcagtccg tgtattcgga tatctatgta gtagatgact actggccgga ttttaagcct
      541 tcccatgtgg aggaggccct tgagtggtat agcaggcagg atgtaacgct gggaggatag
      601 ggaagtttct ccggcaaagg aaactaatac acctcatact gtgcccgatt cggctgtttc
      661 gtatccccgt ttttatccca cacgccgtct gttccgatcc agtgatagag atcggaggat
      721 tttgaacgga cgtagcattg ggaaaccata tatcctgcgc cgtcaaagaa gtaccatttt
      781 ccatcaatct gcctccaggc catctgtggg aaagcgccgt ttggatacag aaagaggtgt
      841 tttccatttt cttccttcca gatgccgtcg ctgtggaacc aggcttcctc tggggtgatg
      901 acaaggggct ctgcatcgga gcaccaaccg ctgtttttcc tgtcttttct gttatatgcc
      961 cggacacgga aggtatagga accttcattg ttgatccaat tttcaaagct gcaggaggca
     1021 gcagagctgg taagggtggt aagagtgcgt ctgtccttaa gaagttgtat ttcatactgg
     1081 tctgccccat ctacctcatc ccagacggct gtatgaccgc tccagtccgc tgtaagggga
     1141 gcaggaatgg ccccgtcgat tctgggcagg cggacatgaa ggatcagagt gcttccctcg
     1201 tcttctgtgc gggcgctctg atactggacg ccgtctccgg aaagatggaa aaggcttctg
     1261 gcggcagtag ggaaataata cccttcattt gcagaaagtt ccacttctcc cacaggccat
     1321 tctccgtagc tccaggtatc gtcatccttc agatatccgg aaccttccac ggtgaactgg
     1381 ctgctgctgg aaacggcggt cagttctccg acttcttttc cgccttcagg actttgatcg
     1441 taactgaaaa taagggaaac atcggtgatc cgttcttctc ccatggcagt aaaggtcagg
     1501 cccagggaaa taagggcagc tgtgcagagg gtctgcagcg tatgtttctt cataagaatt
     1561 ctcctttccg gatatgcatt gcccggcaca caggaagagc gcacgctttc cccgctgccg
     1621 ggcaataacc acagtatgtc atattcacga agagatcagt caaaaataga acctaccaac
     1681 attttcagca tggattcaga ggtgcggatc ttaagctggt ttgccccgca gcactggatg
     1741 gtggtagcca tcagggtgtc ctttaattcc ggttcacaga tgcttaccat gttggcgcag
     1801 gattcgatga tggcggttaa acggccagct ttttcgataa agcgggaaat gtgctcctct
     1861 gtaagccaaa gggaaaattc ggcaaggggt tcgccgttta attcattcca ctccgttttg
     1921 gaaaatccat aggtttcttt gagactgttc atcagttgat tgatatccat ttcatactcc
     1981 tcatttgtat aagatcagtc cccttaaggg gcagtataca ataatatatg acatacgatg
     2041 ggatatttca agtgaaaacg ggcaaaaatt caatgattgt aaagctgttt gatgatttct
     2101 acggttcggt ccatgcccag acggcttaca tttaagagca tctggtggga atcaatggaa
     2161 ccccatttct ggtgcgtgta gttttcataa taatgctttc tgcgcttatc cgtggaatgg
     2221 atggcattta tggcatcttt ttcggagaaa tgataccgtt ccatgatccg gtggatccga
     2281 tcctccatat tggcgcagat aaacaggtcg atgacgtcat ttctggagcg cagggcataa
     2341 ttgccgcagc gtcctacgat gacgcagggg cctttccggg caagcgtttc aatgatgatc
     2401 gtctgggtta cataggtact gtccggcaga gaaagtccgt atccggtgac ggaattgggt
     2461 tcccgcggta catggtaatc agccagaaat cccatcaggg ccgattcgtc tactttttct
     2521 acgcttactg atgcatggcc cattttttct gcagccattt caaccagctc ccggtcataa
     2581 aaggggatat ttaattcttt ggcggtacgc atcgcagcct cccgtccgcc gcttccaaat
     2641 tgccgtgcaa tggttatgat cttaccgctc atagtaccgc tcctttcatt tcactgcatc
     2701 tgaaaagtga aaatgcacaa tacaatgttc tgtaagttca ttatacatta cataacgcac
     2761 aaagggaaga gggattttgg cgtctatgta tggttgtcat gggcatcagg ctttttgcag
     2821 gaagcgtccg cctgtttctg cgccttccgc tgttcccgca tcatttccca gatggtatca
     2881 tgcatccatt ttacggtatc gctgagaatt ttgttttccg tatctaaccg catgatttcc
     2941 atattcattt ctgcaatgac ttcacggctg ttgtatttct tatggggagc tgttatgcgc
     3001 tccatcaggg aaggattttg ctttagaatt tcatttccaa ggtaatcatc gcgctcctga
     3061 cagctcatat tcataaactc tttgtaatgc atccgcacca tattcatagt atcctttgaa
     3121 ttacatctgg gacactgggg atgacgggat gtctgataat atgcataata tccgcatttg
     3181 ggacagtaaa aagcagcaag ctctctcatt tatataatcc tcctactaca aacttgatca
     3241 gtctataata aataacgcat gagagcggaa aatataccgc tgttttttaa aatatttcaa
     3301 aaaaagcaaa ataaacaaaa aaaatggcga atttctggaa aatcagacgt tctgttgaat
     3361 aagtttagca tttatgggcg aatttgtaaa gaggagctta atgtaaaggc catatcaggg
     3421 acagtttgta taaaaactga tctgatatgg cctttaagga aatattttta ttttccgccc
     3481 gtcacagggt gaacggatgt gggtgagagg cggtgggtta ctggatgatg ccgctggtgt
     3541 ttacggtcca gatggtgtca ttttcatcct tgaaatcctt ataacctgct ccaagctcag
     3601 gttttgcgtt ggatttggag gaggagccgg ctttctggat agcaccgttg gtattgatca
     3661 gatagcgggt accgtccaga tcagccggaa ccagacgcag gtcgggatca gcctcctgac
     3721 ggagtccgtg aatgtagagg gtgttatcct tgatgccgtg atatccctgt ccctttttgg
     3781 agccgtcggt gtggaagtac cagttctggg tttcacccag gtcttcgtca tagattgtct
     3841 gcttccctgt tttcatagcg ccgtcttcgc cgaaatagaa gttggcagag gcgccgtcgc
     3901 cgatgtttac agactgcttt ccggtctgca tttcgccttt tgtgttaaag gcgtacttct
     3961 tggagtcgat ggagaagagc tccaggccgg actgagcgtg atggggaacg ccgtttttaa
     4021 agtaaaactt gtaaacctcg gcttcctcgc tgatgccttc agcgccttca atggttctcc
     4081 agccggaggc acgctttccg tcctcatcat aatactgata accggaaata gaggaggcgt
     4141 cggctttgac agcctcggaa gcggtggcgg cgttttctgc tgcgggaagc ttaaaccagc
     4201 cggtctgcat ctttccgtct acaaaggtat agtaattgcc gtttattttc ttatcaaccg
     4261 catgttcgat gcgctttccg ctgttatcga agtagtacca ggattcaatg atctccggat
     4321 cgtctgtttc atcttctaaa agcacccagc cggtcttgcg gcttccatct tcgcccagat
     4381 agtaattgaa gccgtctacc tgaatggagc cggtttccat gatggaatcc tcgttgaaat
     4441 aataccactg tccgttaatg gaggcccagg tggaggttaa ggctcttccg tctcttccat
     4501 aataatagta gagaaactcc ggctcatcct gttcagacca gtaatcttca ttttctacgg
     4561 aaatccattt catagtaacg cgttttccgt cttcatctac atagtattcg tcaatctgag
     4621 tggaagtggc acggattccg tcagaatcca gatagtacca gtcgtcacca gacttcttcc
     4681 aggtgtcggt aaggggctcc ccgtaggagt cataatagta ccagttgccg ttttcctgtg
     4741 cccagcctgc atttttggca tatgcaggaa aagaaagggc tgcagcaaga gctgcggtgg
     4801 ataatacagc cagaagcttt gtctgttttt tcataataat aattctctcc ttttgaaatg
     4861 tgtgtgtcgg ccgaaaatcc gttgttgcat tacgaggctc attatagcgg gtgagcggcc
     4921 ggatttccag tgaggcttac tggaaggaat ggaaggactt tcccggcagc cgggaacccc
     4981 tgccagggaa aatggtagta ggctttttaa tggttttgtg atatactcac cgtatgatgt
     5041 tgggggcaga tgtgctgtcc cctttgat
//
LOCUS       DBMDUH010000175         4860 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_6567_length_4860_cov_6.847867, whole genome shotgun sequence.
ACCESSION   DBMDUH010000175 DBMDUH010000000
VERSION     DBMDUH010000175.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4860)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6567_length_4860_cov_6.847867"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="ACZPCG_11800"
     CDS             complement(<1..677)
                     /locus_tag="ACZPCG_11800"
                     /inference="COORDINATES: protein motif:HMM:NF024214.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR1285769.1"
                     /translation="MEKTGAVIVAAGHKNRGTAFLPMLPVGDSTVIRRIIITMRRVGI
                     DPIVVVTGKQGDELEKHISDLEVISLRNPDYETEQMFDSISMGLRYIRGLCTQTLILP
                     AKFPLLLRDTLEKMLSEEGDIIRPVFDGKRGHPVLISEHAAGKVLEYTGNRGLRGALF
                     EAEQCFVTKEVPVEDQGIIFAVETDEDSTEREMEKQKIQVHPEVRLVVRRNDPFFGPL
                     LARFLTVV"
     gene            complement(679..1830)
                     /locus_tag="ACZPCG_11805"
     CDS             complement(679..1830)
                     /locus_tag="ACZPCG_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HIR1285770.1"
                     /translation="MALETEHRLLLGVEIKKKLKEFSLELSFEAEKGCLGILGPSGCG
                     KSMTLKSIAGIVTPDSGRIALQYAQGEAAGGRVLFDSSLKINETPQIRRVGYLFQNYA
                     LFPNMTVEENIAAGLKGKGARAAAGGRKSQACSPQAIKQKVQEMAERFRLIGLEKHYP
                     AQLSGGQQQRTALARILAYEPEILLLDEPFSAMDTYLREKLRLELAEALKDYDGVSIL
                     VTHDRDEAYQLCPEILLMDQGKVLARGKTRDIFAWPQTCKAARLTGCKNISRIRRMGE
                     RHICALDWGGLELMADRLVGEEITAVGIRAHDFEPLSRQKAAAYAYENCIPVEEPQVS
                     EMPFEWYITLKNGLWWKREKTIHTHNSAGVIPEYLRVDPSAILLLKGEL"
     gene            complement(1820..2497)
                     /gene="modB"
                     /locus_tag="ACZPCG_11810"
     CDS             complement(1820..2497)
                     /gene="modB"
                     /locus_tag="ACZPCG_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HIR1285771.1"
                     /translation="MDWSPVLISMKTASLSIFITFFLGIFAAGVVIRLKNETSKIICD
                     GILTLPLMLPPTVAGFFLLYLFGVKRPIGQFFIDFFGVKIAFSWGATVLAAVVMSFPL
                     MYRSARGAFEQVDPDLTAAARTLGMSEWEIFWKVLFANATPGILSGGVLAFARGLGEF
                     GATSMIAGNIAGKTRTLPMAVYSEVAAGNMGAADRYVAVIVVIAFISILLMNLGALRA
                     GGRKHGA"
     gene            complement(2525..3415)
                     /gene="modA"
                     /locus_tag="ACZPCG_11815"
     CDS             complement(2525..3415)
                     /gene="modA"
                     /locus_tag="ACZPCG_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR1285772.1"
                     /translation="MRKQLYAVAAAILAAGMLAGCSGGAKETEEQTSVQAEKETETAK
                     IPAAAEAETEAASEKEETELLVAAAASLKNAYEEELIPMFEAQYPGVIVTGVYDSSGK
                     LQTQIEEGLEADVFMSAATKQMDALNEKGLIAEDTIADLLENKIVLIVPTGNEAGLEA
                     FEDIEKASSIALGDPASVPAGQYAEEVLTSLGIWDRIQDKVSFGTNVTEVLNQVAASS
                     ADAGIVYATDAASMAEQVDVIAEAPEGSLKKKVIYPVAVLKGSAHSEEAADFVEFLKT
                     EEAMKVFEAYGFSKGNEILP"
     gene            complement(3471..3971)
                     /locus_tag="ACZPCG_11820"
                     /pseudo
     CDS             complement(3471..3971)
                     /locus_tag="ACZPCG_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
     gene            complement(4000..>4860)
                     /locus_tag="ACZPCG_11825"
     CDS             complement(4000..>4860)
                     /locus_tag="ACZPCG_11825"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002593452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990140 - molybdopterin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0061798 - GTP 3',8'-cyclase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HIR1285773.1"
                     /translation="GEPLVRRDCPQLVRMLKNVPGIEKVTLTTNGVLLEQYLGELLNA
                     GLDAVNISLDTLDPGLYETITGADALDKVLGIISRADRLPIPVKVNAVSIDFDNWKDG
                     KERDKNGWRAMAALAEQYPVDVRFIEMMPIGYGKQFQGIDHRTLLLQLKEMYPDLETD
                     HEVHGFGPAVYYKSQRFKGSIGLISAIHGKFCSSCNRVRLTAQGYLKTCLCYEDGVDL
                     KQVLREEISRPEKESHFIWPEGLDVRDEGLQSRLRTVMEKTILKKPMAHCFETPGRIT
                     ESHNMSSIGG"
BASE COUNT         1127 a         1467 c          996 g         1270 t
ORIGIN      
        1 accacggtga gaaaacgggc aagcagcggc ccaaagaagg gatcatttct ccgaacgacc
       61 agacgcacct ccggatgcac ctggatcttc tgtttttcca tttcccgttc cgtactgtct
      121 tcatccgttt ctactgcaaa gataattccc tgatcttcca caggtacttc ctttgtcaca
      181 aagcactgct ccgcttcaaa aagtgctccc cgcaagcccc gatttcccgt gtattcaagc
      241 actttcccgg ctgcgtgctc cgatataaga accggatggc ctctttttcc gtcaaaaacc
      301 ggccggataa tatcgccctc ttctgaaagc attttttcca gcgtatcacg caaaagaagc
      361 ggaaatttag caggaaggat cagtgtctgt gtacacagtc ctctgatgta gcgaagaccc
      421 atggaaatgc tgtcaaacat ctgttccgtt tcgtaatcgg gattgcgcag ggaaataacc
      481 tccagatcgc ttatatgctt ctccagctca tctccctgtt ttcccgtcac taccacaata
      541 ggatcaatcc ccacccgcct catcgtaata atgatccgac ggatcacggt gctgtcccct
      601 accgggagca tgggcaaaaa ggccgttcct ctgtttttat ggcctgccgc cacaatgact
      661 gctcctgttt tttccatatc agagttctcc ttttaacagt aaaatagccg acggatctac
      721 ccgaagatac tccgggatca ctccggcaga attgtgggta tgaatcgtct tttctcgttt
      781 ccaccacaga ccgtttttca gggtaatata ccactcaaag ggcatttcag acacctgggg
      841 ttcttccaca ggaatacagt tttcatatgc gtatgcagct gccttctgtc tgcttagcgg
      901 ctcaaaatca tgagcccgga tccccactgc agtaatctcc tcccccacca gacggtctgc
      961 catcaactct aagccgcccc agtccaaagc gcagatatgc cgttcaccca tccgcctgat
     1021 cctggaaata tttttgcagc ccgtaagtct tgcagccttg caggtttggg gccaggcaaa
     1081 aatatctctc gtctttcccc tggccagaac ctttccctga tccatcaaaa gtatctccgg
     1141 acagagctgg tatgcctcgt ccctgtcatg agttaccaga atggaaacgc cgtcataatc
     1201 ctttaatgct tctgccagct ccagacgcag cttttccctc agatatgtat ccattgctga
     1261 aaacggttca tctaaaagca ggatttccgg ttcataggcc agaatccggg ccagtgctgt
     1321 tctctgctgc tggccgccgg acaactgagc gggataatgc ttttccagtc cgatcagccg
     1381 gaaacgctcc gccatctcct gcaccttctg cttaatggcc tgaggggagc aggcctggga
     1441 cttcctgccc cccgctgcag cccttgcgcc ctttcccttt aagcctgcgg caatattttc
     1501 ctccaccgtc atattgggga acagggcgta attctggaac agatagccta cccggcggat
     1561 ctgaggcgtt tcattgatct taagggagga atcaaacaga acccgtcccc cggctgcctc
     1621 cccctgagca tattgaaggg caatccggcc ggaatccggc gtcacaatgc ctgcaatgga
     1681 ttttaaagtc atgctctttc cacagcccga aggtcctaaa atacccagac agcctttttc
     1741 cgcctcgaag gaaagctcca gagaaaattc cttcagcttc tttttgattt caactcccag
     1801 caaaagcctg tgttctgtct caagcgccat gctttcttcc tcctgccctt aaggctccca
     1861 gattcataag cagaatagag ataaaggcaa tgaccacaat gacggccaca taccggtctg
     1921 cagcccccat attgcctgcg gccacctctg aatacactgc cattggtagg gttcttgtct
     1981 ttcccgcaat attacccgcg atcatagaag tggctccaaa ttctcccaga cctctggcaa
     2041 aggccaggac accgccgctt aagatgccgg gagtggcatt ggcaaaaagc accttccaga
     2101 aaatttccca ctcactcatc cccagagtcc ttgcagccgc agtcagatcc ggatccacct
     2161 gctcgaatgc ccccctggca gagcggtaca tcagcggaaa tgacatcacc accgccgcca
     2221 gtaccgtagc tccccaggaa aaagcaatct ttactccgaa aaaatcaata aaaaactgtc
     2281 caatggggcg ttttacccca aacagataga gcagaaaaaa gccggccacc gtaggcggaa
     2341 gcatcagggg aagagtcaga ataccgtcac atatgatttt tgacgtctca tttttcagac
     2401 ggatcacaac ccccgctgca aaaatcccca gaaaaaaggt gataaagatg gacaggctgg
     2461 ctgtcttcat ggatatcaga accggtgacc agtccataat acgtcctcct tttcatttac
     2521 agatttaagg caggatttca tttcccttgg agaagccata agcctcaaat accttcatgg
     2581 cttcctctgt ttttaaaaac tccacaaaat ctgccgcttc ttcagaatgt gcgctgcctt
     2641 ttaacacagc tacaggataa attaccttct ttttcagact tccctcggga gcctcagcaa
     2701 ttacatctac ctgctctgcc atgctggctg catctgtggc atatacgata cctgcgtctg
     2761 cactggaagc cgctacctgg tttaaaacct ctgttacatt ggttccaaag cttaccttat
     2821 cctggatccg atcccagatt ccaagacttg tgagcacttc ctctgcatac tgaccggccg
     2881 gtacgctggc cgggtcgccc agtgcgatag aggatgcttt ttcaatatct tcaaaagctt
     2941 caagccctgc ttcatttcct gtgggaacaa tcagaacgat cttattttcc agaagatccg
     3001 caatcgtatc ctcagcaatc aacccctttt catttaaagc atccatctgc tttgtggctg
     3061 cggacataaa tacatctgcc tccagtcctt cttcgatctg ggtctgaagc ttaccggagc
     3121 tgtcatagac gcctgttact ataacacccg gatactgagc ctcaaacatg gggatcagtt
     3181 cctcttcata ggcatttttt aaactggcgg cagctgccac taaaagctct gtctcttctt
     3241 tctctgaagc ggcttctgtt tctgcctcag cggctgccgg aatcttcgcg gtctctgttt
     3301 ccttctctgc ctgaacactt gtctgttcct cagtctcctt tgctcctcct gagcatcctg
     3361 ccagcatccc tgctgctaaa attgctgctg ccactgcata aagctgtttt ctcattttcc
     3421 gctcctcttc tttctgattt tttccagtta tttactcatt cctattggcc ttatctggca
     3481 cattctgagg cactgcctct taaaatccga atcccgtggt caagagaatc caaaatataa
     3541 cccagactct cccttaccgc cttcggactt cccggaaggt tgacgattac ggtctgtctg
     3601 cggatcactg atactccacg gctgagcatg gcccggttcg tcactgccat ggactgatac
     3661 cggatggcct cggcaattcc gggagccaac cggtctgcca ctgccagagt cgcctccgga
     3721 gtctgatccc gcatggaaaa tcctgtaccg ccgcttgtaa ccaccagatc tgcccggact
     3781 tcatctgcca gacgaatcag ctctgtctta agttcctccg gctcatcagg aatgatcagc
     3841 gtctccacta cctcatagcc tgcctcctcc agcatctctt ttgctgcagg gccgctttca
     3901 tccacacgct ctcccctggc tcccttatca ctcaccgtaa taacagctgc cttaaatgac
     3961 ttttcctcag atccgtttct ctccttcgca tccatccttt tatcctccga tcgagctcat
     4021 attatgtgat tccgtaatcc ttcccggtgt ttcaaagcag tgggccatgg gctttttaag
     4081 gatcgttttc tccatcacag tcctcaaacg gctctgaagc ccttcgtcac gaacatccag
     4141 cccttccggc cagatgaagt ggctttcctt ttccggccgg gatatctcct ctctgagcac
     4201 ctgtttcaga tccacgccat cctcataaca cagacaggtt ttcaaatatc cctgagcggt
     4261 cagacgcacc cggttgcagg aagaacagaa ctttccgtga atagcgctga tcaagccaat
     4321 gctgccttta aacctttggg atttataata aacagccggg ccaaaaccat ggacttcatg
     4381 gtctgtttca agatccggat acatttcctt cagctgcaaa agcagcgttc tgtgatcaat
     4441 gccctgaaac tgctttccat aaccaatggg catcatttca ataaagcgca catcaaccgg
     4501 atactgctct gccagtgctg ccatggcccg ccagccgttc ttgtcccgct cttttccatc
     4561 cttccagttg tcgaaatcaa tggataccgc attgaccttc accgggatgg gaagcctgtc
     4621 tgccctgctt atgattccca gaaccttatc cagcgcatct gccccggtaa tggtctcata
     4681 aagccccgga tccagagtat ccagactgat attgaccgca tccagccccg cattcaaaag
     4741 ctcacccaga tactgttcca gaagaacacc gttggtggtg agagttacct tctcaatacc
     4801 ggggacattt ttgagcatcc gtactaactg ggggcagtcc ctgcgcacca gcggctctcc
//
LOCUS       DBMDUH010000176         4850 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_6580_length_4850_cov_6.293325, whole genome shotgun sequence.
ACCESSION   DBMDUH010000176 DBMDUH010000000
VERSION     DBMDUH010000176.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 4850)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4850)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4850
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6580_length_4850_cov_6.293325"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..143)
                     /locus_tag="ACZPCG_11830"
     CDS             complement(<1..143)
                     /locus_tag="ACZPCG_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008723835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin-like domain-containing protein"
                     /protein_id="HIR1285774.1"
                     /translation="MKKWVCTVCGYVYEGEQAPEKCPQCGVPASKFKEQAADQMAWAC
                     EHEV"
     gene            374..682
                     /locus_tag="ACZPCG_11835"
     CDS             374..682
                     /locus_tag="ACZPCG_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285775.1"
                     /translation="MKTENTLARNLDFAADKASYDAACKRLLANKEILAWIMKSCIEE
                     YRECSVKDIAEYYIEEEPQIAHTAVNSDERSANGSDTIRNLQMDTMKRYARFTRSGSV
                     "
     gene            679..1161
                     /locus_tag="ACZPCG_11840"
     CDS             679..1161
                     /locus_tag="ACZPCG_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285776.1"
                     /translation="MNPPKRRKNTINRYSITEECMVGEEKEKKENYDLMTAVIICLGD
                     SENEHYSGIIKLLDVLLSDEQDPQKKKHILEEDFSVKMTRDLEREVSLMCNLSKGIED
                     RGMEKGLEQGLEQGMEHGLLSSIRNLMETMSWSSGQAMDALKIPRENRERYEQLLKQL
                     "
     gene            complement(1229..2644)
                     /locus_tag="ACZPCG_11845"
     CDS             complement(1229..2644)
                     /locus_tag="ACZPCG_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005331574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1285777.1"
                     /translation="MNQFPSQGQTGSASNVPANPLGTLPINQLLARFAIPSIIAMLVS
                     ALYNIVDQFFIGRSVGMLGNAATNVAFPLTITCTALSLMFGIGGAANFNLSMGHGERE
                     DAARYAGNAVFMLFFTGLILCLGVRLFLKPLMIAFGATAEVLDYSLVYTGITSLGFPF
                     LILTTGGSNLIRADGSPRFSMVCTLTGALINTILDPLFIFTFHMGMAGAALATIIGQI
                     VSGIMVIIYLTRFKTVHLTFDYLRPIPSHIKAIISLGMAPFFNQIAMMVVQIVMNNVL
                     RYYGGQSHYGSEIPLACAGIISKVNMIFFSLVIGLSQGLQPIVSFNYGAGKYRRVRSA
                     YLKAVCIATCISSVSFLCFQLIPRQIISIFGSGSEEYFRFAEQYFRIFLFFTFLNGLQ
                     PITANYFTSIGKAGTGIFISLTRQIIFLLPLILILPMFLGIEGVMYSAPVADLMAAVL
                     AVFFVSRELKRMKQMEHLSEA"
     gene            complement(2666..4006)
                     /locus_tag="ACZPCG_11850"
     CDS             complement(2666..4006)
                     /locus_tag="ACZPCG_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HIR1285778.1"
                     /translation="MSHSSTTHRDSWKSNWGFVLACIGSAVGMGNIWMFPTRVSKYGG
                     GTFLLPYFLFVIIIGLSGVIGEMAFGRATRSGPIGAFGQAIASRGKNPKIGQAIGYLP
                     VIGSLALAIGYSVIVGWILKYAAGSLTGSVLAQADIEGFGQAFGQMAAPFGNNFWQTV
                     GIVITFIIMVCGISGGIEKINKVIMPLFFFLFLGLAVYMAFQPGAAEGYRYIFRIEPE
                     GLADPMTWVFALGQAFFSLSLAGNGTLIYGSYLKDSEDVTSAARKVAFFDTMAALLAA
                     LVIIPAMATTGSQLNEGGPGLIFIYLPNLFKSMPGSRILVIVFFTAVFFAGISSIINL
                     FEAPIATLEQHFKLSRRVSVTIAASLALAVGLCIQGIVSGWMDFVSIYICPLGAGLAG
                     IMFFWVYGSDFAKKEAEKGRPKPLGSWFRFMTAYVFCGLTIAVFILGIIFGGIG"
     gene            complement(4247..4792)
                     /locus_tag="ACZPCG_11855"
     CDS             complement(4247..4792)
                     /locus_tag="ACZPCG_11855"
                     /EC_number="1.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003021715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HIR1285779.1"
                     /translation="MKKWVCTVCGYVYEGEQAPEKCPQCGVPASKFKEQAADQMAWAC
                     EHEVGVAQGSPEDIMADLRANFEGECSEVGMYLAMSRVAHREGYPEIGLYWEKAALEE
                     AEHAAKFAELLGEVITSSTKKNLEMRVAAENGATAGKFDLAKRAKAANLDAIHDTVHE
                     MAKDEARHGRAFEGLLKRYFG"
BASE COUNT         1468 a         1066 c         1197 g         1119 t
ORIGIN      
        1 acctcatgct cgcaagccca agccatctga tcagcagcct gctccttgaa cttggaagca
       61 ggaacgccgc actgaggaca tttctcagga gcctgctcac cctcataaac gtaaccgcaa
      121 actgtacaaa cccatttctt catagtcttg atctccttta ttatatagtg tgtgggaacc
      181 ggggatgccc cggaaccctg ttaacattgc gaatcgataa tagtaatgat tactgatatc
      241 aatataaatg atttatgctg ttttgtcaac agcttttttt aaaaaattta agtttcgttt
      301 tcatagtctg gcgtggatat tgatagtatg gtagaattta agaaggaaaa cggttgaaag
      361 gggatgcagg caagtgaaaa cagaaaatac tttagcaaga aatctggatt ttgctgcaga
      421 taaggcatct tatgacgcgg cctgtaaacg gcttctggct aataaagaga ttttagcctg
      481 gattatgaaa agctgtattg aggaatatag agaatgcagt gttaaggaca ttgcagagta
      541 ttatattgaa gaggaaccgc agattgccca tacagcagta aattcggatg aacggtccgc
      601 gaatggttcc gatacgattc ggaatttaca gatggacact atgaaaagat atgcaaggtt
      661 tactcgatct ggatctgtat gaatccacct aaacggcgga aaaatacgat taaccgatat
      721 tcgattacag aagagtgcat ggtaggagag gaaaaggaga agaaggaaaa ttatgacctg
      781 atgacggcag ttataatctg tctgggagat tcagagaatg aacattattc aggtattata
      841 aagcttttag atgtccttct ttctgatgag caggatccgc agaagaagaa acatatttta
      901 gaggaagatt tttcagttaa gatgacaagg gatttagaac gggaggtatc gctcatgtgt
      961 aatcttagta aaggaattga agacagggga atggaaaaag gattggagca ggggctggaa
     1021 cagggcatgg aacatggtct tctttcctcc atccgcaatc tgatggagac catgagctgg
     1081 tcctccggtc aggcgatgga tgcccttaag attcccaggg aaaaccggga acggtatgaa
     1141 cagcttttaa aacagctata gtcagataaa tgtaaaatcc atacagcccc ggcagatata
     1201 caatgatact gtcggggctg tgtggatttt atgcttcaga cagatgctcc atctgtttca
     1261 ttcgctttag ctctctggac acaaagaaga ctgccaaaac agcggccatc agatctgcca
     1321 caggagccga gtacataacg ccctcaattc ctaagaacat aggaagaatg aggattagag
     1381 gcagaaggaa gataatctgg cgtgtgaggg atataaatat acccgtacct gcctttccaa
     1441 tggaggtaaa atagttggcc gtaatgggct gaaggccatt taaaaatgta aagaaaagga
     1501 aaatacggaa atactgctct gcaaagcgga aatattcctc acttcctgaa ccgaaaatgg
     1561 agatgatctg tctggggatc agctgaaagc ataaaaagga tacagaggaa atacaggtgg
     1621 cgatacatac cgctttcaga taggcggaac gtacacggcg gtattttcct gctccgtagt
     1681 taaagctgac aatgggctgc agtccctggg aaagaccgat gaccagggag aagaagatca
     1741 tgtttacctt gctgatgatt ccggcacagg caagaggaat ctcactgccg taatgagact
     1801 gtccgccgta atagcgaagt acattattca taacaatctg taccaccatc attgcaatct
     1861 ggttaaagaa gggtgccatt cccagggata tgatcgcttt aatgtgggaa ggaatgggac
     1921 gaaggtagtc aaatgtcaaa tggactgttt taaagcgggt cagatagatg attaccatga
     1981 taccggatac aatctggccg ataatggttg ccagagcagc accggccatt cccatatgaa
     2041 aagtaaagat aaacaaaggg tctaagatcg tgttgatcag tgcgcctgta agtgtgcaga
     2101 ccatggagaa acggggactt ccgtctgcac ggatcaggtt agagcctcca gtagtaagga
     2161 tcaggaatgg aaaaccaagg gaagtaatgc ctgtgtatac aagagagtaa tctaaaactt
     2221 ctgctgtggc tccaaaggct atcatgagag gctttaaaaa taagcgtact cccaggcaga
     2281 ggatcaatcc tgtaaaaaac agcataaaaa cagcatttcc cgcataacgg gcagcatctt
     2341 cccgttcgcc atggcccatg gaaagattaa agttagctgc gccgccgatg ccaaacatca
     2401 gggaaagggc ggtacaggtg atggttagag gaaatgcaac attggtagca gcatttccaa
     2461 gcattcccac actgcggccg ataaaaaact gatctacgat attatacagg gcactgacca
     2521 gcatggctat gatgctggga atggcaaagc gggctaaaag ctggttaata ggcagagtgc
     2581 ccaggggatt tgcagggaca ttggaagctg atcccgtctg tccctgtgat ggaaattgat
     2641 tcattttaga tacctctctt ttgtatcagc caatgcctcc gaagataatt cccagtataa
     2701 atactgcgat ggtaagtccg cagaagacat aggcggtcat aaagcggaac caggaaccaa
     2761 ggggttttgg gcggcccttt tccgcttctt tttttgcaaa atcgctgcca tatacccaga
     2821 aaaacatgat tcctgccagg ccggcgccca atggacagat atagatagaa acgaagtcca
     2881 tccagccgga tacgattccc tggatgcaaa ggcctactgc gagagccagc gacgcagcaa
     2941 tagtgacaga tacacggcgg ctcagtttaa aatgctgttc caaagtggca ataggagctt
     3001 caaacaggtt gataatggat gaaataccgg caaaaaatac ggctgtaaag aaaacgatta
     3061 ccaggatgcg gcttccgggc atggatttaa acaggttggg cagataaata aagataagtc
     3121 cgggaccgcc ttcatttaac tgagaaccgg tggtagccat ggcgggaatg ataacaaggg
     3181 ctgcaagaag agcggccatg gtgtcaaaga aggccacttt gcgggctgca ctggtaacat
     3241 cctcgctgtc ttttaaatag gagccgtaga tcagagtgcc gtttcctgcc agagacaggg
     3301 agaaaaatgc ctgtccaagg gcaaaaaccc aggtcatggg atctgccagt ccttccggtt
     3361 caatacggaa gatgtaacga tatccttctg ccgcccccgg ctggaaagcc atatatacag
     3421 caagccctaa aaacaggaaa aagaacaggg gcataataac cttattgatt ttttcaattc
     3481 ctcctgaaat accacagacc atgatgataa aggttattac gatgcctaca gtctgccaga
     3541 agttattgcc aaagggagct gccatctgcc caaaggcttg tccgaagcct tcaatatctg
     3601 cttgggccag caccgagccg gtaagggaac ctgcggcgta tttgagaatc cagccaacga
     3661 tgacagaata accaatcgcc agagccagag atccgatcac gggaagatag ccgatcgcct
     3721 gtccgatctt gggatttttc ccgcggcttg ctatggcctg gccaaaggct ccgatggggc
     3781 cggaacgggt ggcccgcccg aatgccattt caccgatgac gccggaaaga ccaatgatga
     3841 ttacaaaaag aaaatagggc agcaagaagg tgccgcctcc gtactttgag accctggtgg
     3901 ggaacatcca gatattgccc atgcctactg cagagccgat gcaggccagg acgaaccccc
     3961 agttggactt ccaggaatcc ctatgggttg tacttgagtg actcacaaaa aacctcctgt
     4021 agcgccataa aggagtgatg aggcgctgaa aaatgatatg ttcagtgaaa acgcatatat
     4081 cacgattcac caattctatt gtaaccccaa acagaacgtt tgtctatcag gtgatggaag
     4141 gcgaagagga gggaaataac agtatacagc atggatgctg gaaaatgaag aaagagggaa
     4201 aagacgaatc ctttccctct cagatatcag gtttatcgaa ttttaattag ccaaagtatc
     4261 tctttaacag accctcaaat gctctgccgt gtctagcctc atccttagcc atctcatgaa
     4321 cagtgtcatg gattgcatcc aggttagcag ccttagctct cttagccagg tcgaacttgc
     4381 cggcagtagc gccgttctca gcagcaactc tcatctccag gttcttcttg gtggagcttg
     4441 taataacctc gcctaagagt tcagcaaact tagcagcatg ctcagcctct tccagagcag
     4501 ccttctccca gtacaggccg atctcaggat agccctctct gtgagcgact ctggacatag
     4561 ccaggtacat accaacctca gagcactcgc cctcaaagtt agctcttaag tcagccatga
     4621 tatcctcagg ggaaccctga gcaacgccaa cctcatgctc gcaagcccaa gccatctgat
     4681 cagcagcctg ctccttgaac ttggaagcag gaacgccgca ctgaggacat ttctcaggag
     4741 cctgctcacc ttcataaacg taaccgcaaa ctgtacaaac ccatttcttc atagtcttga
     4801 tctcctttat tatatagtgt gtgggaaccg gggatgcccc ggaaccctgt
//
LOCUS       DBMDUH010000177         4830 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_6607_length_4830_cov_4.423134, whole genome shotgun sequence.
ACCESSION   DBMDUH010000177 DBMDUH010000000
VERSION     DBMDUH010000177.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 4830)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4830)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4830
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6607_length_4830_cov_4.423134"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="ACZPCG_11860"
     CDS             <1..188
                     /locus_tag="ACZPCG_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285780.1"
                     /translation="LSDNWSLAAFASIQSVTEVSPKVSPIFKKHSIYKEKGDFLTVSK
                     TVDGGSSPSSPADYLKK"
     gene            277..1416
                     /locus_tag="ACZPCG_11865"
     CDS             277..1416
                     /locus_tag="ACZPCG_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4317 domain-containing protein"
                     /protein_id="HIR1285781.1"
                     /translation="MNKKECNEIKKLFTPANCAISRICGCYVDSEKNKKTELKEAFLS
                     LPEEEAFKYFTIFRNGLSGTVGKNLLNMEFPLHTESEGGTQNFLLKLRDSELKDEALI
                     EEFYDKIIANFDYGENYYIILIHCAYDIPAKATDGSEMFDASDYVYEFIQCTICPVKL
                     SKAGLCYNSMTNAIENRIRDWLVEAPINSFLFPAFNDRNTDIHSLLFYAKNPEQLPDS
                     LIDEVLGCGIPMSAKSQKETFQAIVEETLGENCDFETVKNIHENLSELVEETKDEPVP
                     LTLDKYQVKKLLENNGASPEKLEEFEERYKDVENTPETSFVAANVVNTKIFEIKTPDV
                     TIKVAPDKTYLVENRMIDGRPCIVIAINEHVEINGISCRPIAVQK"
     gene            1534..1956
                     /locus_tag="ACZPCG_11870"
     CDS             1534..1956
                     /locus_tag="ACZPCG_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUMOD1 domain-containing DNA-binding protein"
                     /protein_id="HIR1285782.1"
                     /translation="MSRTIKAGHVYRQYSRDGMFIADYKSSRLAYEATGVSLGSIARA
                     AHGERKTGGGYLWRVVPENTPEDPISVDLTSRIGYHDKRPLIQMTLDGEPVAEFISIA
                     HASRSLGISRRSLSCALTGAQKTAGGFIWKEKESEENK"
     gene            2131..3369
                     /locus_tag="ACZPCG_11875"
     CDS             2131..3369
                     /locus_tag="ACZPCG_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008725137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR1285783.1"
                     /translation="MGKKRNIIVGQSGGPTAVINSSLAGVYKTAKERGFHKVYGMLHG
                     IEGFLDEMYVDLSTQIHSDMDIELLKRTPSAFLGSCRFKLPEIHENPEIYEKIFSIMN
                     KLEIEVFIYIGGNDSMDTIKKLSDYAIVKGHEQKFLGVPKTIDNDLALTDHTPGFGSA
                     AKYIAASTKEVIRDAMGLSYKRKMITIMEIMGRNAGWLTGATALAKTEDCCGPDLIYL
                     PEIPFDVDRFLKKCSDLLEKKPSIVIAVSEGIKVPNGRYVCQLAGGSDYVDAFGHRQL
                     AGTAGYLASFLAAKTGCKTRAVELSTLQRSASHMASRVDINEAFMVGGAAVKAADEGD
                     SGRMVVIDRVSDDPYMSAAGIYDVHKIANNEKLVPRSWVNREGNYVTREFIDYVEPLI
                     QGDYQPFMVNGLPQHLVLKR"
     gene            3376..4065
                     /locus_tag="ACZPCG_11880"
     CDS             3376..4065
                     /locus_tag="ACZPCG_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285784.1"
                     /translation="MRQWKKWAVFIGTAVCLVLGSLYLYTLFRPGLWMQETFLYRQKD
                     GSFTGADGETDYKMQIRRQDRAAWIEFSVNETKKLYEVRWDPDNSRGKIFEDGALVFE
                     GEAHLVGDTWLLTGDGEEVYFDMQIKNGISTEHSDEELFPDYSCIYRWAVNENCQKRG
                     RVEVLPVIAFFGVLLALDTAFPDLLFKFQHGLAVEGGEPSEFYRMGQFIGRIAMVLGI
                     IVGVAASFHAP"
     gene            4097..4759
                     /locus_tag="ACZPCG_11885"
     CDS             4097..4759
                     /locus_tag="ACZPCG_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285785.1"
                     /translation="MNEWNKEIQNENELQVVISVSKAEELGKWLGILFWMVIPSIAGA
                     VLSNQAVAAWLPFLYLPGRIISMVTLCAYGYILLKLSSESSRYRMAAICCLASVAVDA
                     GSSLVPEGAAMASLIGLLSFPVMILAFYGEYSEYMGHSEVICALDEEMAVKWKRLWKW
                     FVYSFGLMISSVLLVMLNGMLGITALLVGTIGTLIVSVMKLTCLYRTAKIFWEYATES
                     SR"
BASE COUNT         1358 a          959 c         1275 g         1238 t
ORIGIN      
        1 aattgtctga taattggtct ttagcggcat ttgccagtat tcaaagtgtc accgaagtgt
       61 caccgaaagt gtcaccgatt tttaaaaaac atagtattta caaggaaaaa ggggattttc
      121 ttacggtctc caaaaccgta gatgggggtt cgagcccctc ttcccctgct gactatttga
      181 aaaaatagcc caacccgcaa gaagtccgga gtactgtaga gtattccggt tttttgtatt
      241 taaacaagga aatcggaaca ggagatacag aaatttatga ataagaagga atgtaatgag
      301 atcaaaaaac tttttacccc tgcaaactgt gccatcagcc ggatctgcgg ctgctatgta
      361 gattcagaga aaaacaaaaa gacagaatta aaggaagcct tcctttcttt gccggaggag
      421 gaggcattta agtattttac cattttcaga aacgggcttt cgggaacagt ggggaaaaat
      481 cttttaaata tggagtttcc tttacatacg gaatcagaag ggggcaccca gaacttcctt
      541 ttaaagctga gagacagtga attaaaggat gaggcgctca tcgaggagtt ttatgataag
      601 attattgcca attttgatta cggtgagaat tattatatca ttctcatcca ctgtgcctat
      661 gatattcctg caaaggccac agacggttcg gagatgtttg acgcgtctga ttatgtatat
      721 gagtttatcc agtgtaccat ctgtcctgtg aagctttcaa aggcagggct ctgttataat
      781 tccatgacaa atgccattga aaaccgcatc cgggactggc tggtggaggc gcccatcaac
      841 agcttcctgt tcccggcttt caatgaccgg aatacagata ttcacagtct gctcttttat
      901 gccaaaaatc cggagcagct gccggacagc ctgattgacg aggtattagg ctgcgggatt
      961 cccatgtcgg ccaagagcca gaaggaaact tttcaggcga tcgtggagga aacactggga
     1021 gaaaactgtg attttgaaac cgtaaaaaat atccatgaaa atttaagcga actggtagag
     1081 gagacaaagg acgagccagt gcctttgact ctggataagt accaggtaaa gaagctcctt
     1141 gaaaataacg gcgcatcgcc ggagaagctt gaggagtttg aggagcgcta taaggatgtg
     1201 gaaaatacgc cggaaacaag ctttgtggca gctaatgtgg taaacacaaa gatatttgaa
     1261 ataaaaacac cggacgtgac catcaaggtg gctccggata agacgtattt ggtggaaaac
     1321 aggatgatcg acggacgccc ctgcattgtg attgccatca atgagcatgt ggaaatcaac
     1381 ggtatttcct gtcgtcccat tgcagtacag aaataggctt tctgcacacc caaagtaagg
     1441 cagtctatgc acactttatg ctgtatggac aaaaaaagta atagttgcta taatgactac
     1501 agccatatta caaaaggaag ggtgaactta cagatgagtc gtacaattaa agcaggtcat
     1561 gtatatcgac agtattcccg cgacggaatg tttattgcag attataaaag ctcccgtctg
     1621 gcttacgaag cgacaggcgt atcgttaggt tctatcgcca gagctgccca tggagagcgt
     1681 aaaacaggag gcggttatct gtggcgggtc gttcctgaaa atactcccga agatccgatc
     1741 agcgtagatc ttaccagccg gatcggctac catgacaagc gtccgctgat ccagatgacg
     1801 ttagacggcg aaccggttgc tgaatttatt tctattgctc atgccagccg aagtctgggc
     1861 atcagcagaa gaagcctttc ctgtgctctt accggagcac agaaaacggc cggaggcttt
     1921 atctggaagg aaaaagaatc agaagaaaac aaataaaatt gtataatata cataaaatac
     1981 agcgcaaaat acgggcataa tctgaaaaag tgctggaaaa atcccctttc ctatagtaca
     2041 ataaggacac gaggcagaca gccgggacgg ttaaaggttc cccttcgtct gtaaggttgt
     2101 ctgtccatga acaggaaagg ggattttgtt atgggaaaaa aacgcaatat tatcgtagga
     2161 cagtctggag gccctactgc tgtgatcaat tccagccttg caggtgtata caagactgca
     2221 aaggagagag gcttccataa ggtttacggt atgcttcacg gcattgaagg ttttttggat
     2281 gagatgtatg tggatttgtc cacgcagatt cattcggata tggatattga gctgttaaag
     2341 cgtactccgt ctgccttttt aggttcctgc cgttttaagc tgccggagat ccatgaaaat
     2401 cccgaaatct atgaaaagat cttttccatt atgaataaac tggagatcga agtatttatc
     2461 tacatcggcg gaaatgattc tatggatacc atcaaaaagc tttccgatta tgcgatcgta
     2521 aagggacatg agcagaagtt tttaggtgtt ccaaagacca ttgacaatga tctggcgctt
     2581 acagaccata cccctggctt tggaagcgcg gctaaatata ttgcagcctc cacaaaagaa
     2641 gtgatccggg atgccatggg cctttcctac aagcggaaaa tgattactat catggaaatc
     2701 atgggacgga atgcaggctg gcttacggga gctacggctc ttgcaaagac agaggactgc
     2761 tgcggtcccg atctgatcta ccttccggag attccctttg atgtagaccg gtttctgaaa
     2821 aaatgcagtg acctgctgga aaagaagcct tccattgtaa ttgcagtttc tgagggcatc
     2881 aaggtaccga acggacggta tgtatgccag ctggccggag gaagcgatta tgtagatgcc
     2941 tttggtcaca gacagctggc cggaaccgca ggctatctgg ccagtttcct ggccgccaag
     3001 acaggctgca agaccagagc tgtagagctt tctaccctcc agcgcagcgc ttcccatatg
     3061 gcctcacgcg tggatatcaa tgaagccttt atggtgggag gcgccgcggt aaaagcggca
     3121 gacgaagggg attccgggcg tatggttgtt atcgaccggg tatcggatga cccctatatg
     3181 agcgcggcag gaatttatga tgtgcataag attgcaaata atgaaaagct ggttcccaga
     3241 tcctgggtaa accgggaggg gaattatgta accagagaat ttattgacta cgtagagcct
     3301 ctgatccagg gcgattatca gccatttatg gtaaacgggc ttccccagca tctggtgctg
     3361 aaacggtagg taaatatgag acagtggaaa aaatgggcgg ttttcatcgg aacggccgta
     3421 tgtctggttc ttggttcgct atatctgtat actctgttcc gacccggttt atggatgcag
     3481 gaaacgtttc tgtaccgtca aaaggacggg agctttacag gagcggatgg agagacggat
     3541 tataaaatgc agatccgccg ccaggacagg gctgcatgga ttgagttttc tgttaatgaa
     3601 accaagaagc tttatgaagt cagatgggat cctgataact ccagagggaa gatttttgaa
     3661 gacggggctc tggttttcga gggggaagcc catctggtgg gagatacctg gcttttaacg
     3721 ggggatggag aagaagtata ctttgatatg cagataaaaa acggtatatc tacggagcat
     3781 tccgatgagg agctttttcc tgattacagc tgtatctacc gctgggcggt gaatgagaac
     3841 tgtcagaaac ggggaagggt ggaagtattg cctgtgatcg ctttttttgg tgtactgctg
     3901 gctctggata ctgcattccc ggatctgctt tttaaatttc agcacggcct tgcagtagaa
     3961 ggcggagaac ccagcgagtt ttatcgcatg gggcagttta taggaagaat cgctatggtt
     4021 ttgggaatca ttgtgggggt agcggccagt tttcatgcac cctgatcccg gtaaaaggaa
     4081 caaaggaggg actggtatga acgaatggaa caaagaaatt caaaatgaaa atgaacttca
     4141 ggttgtgatt tctgtttcca aagcagagga gctgggaaaa tggctgggaa tactgttctg
     4201 gatggtaatt cccagtatag caggcgctgt gctgagcaat caggcggtgg cagcctggct
     4261 gccttttttg tatctgccgg ggcggatcat cagtatggtt acgctgtgcg cgtatggcta
     4321 tatactttta aagctgtctt ctgaaagcag ccgctaccgt atggcagcga tttgctgtct
     4381 ggcatccgtt gcagtggatg caggaagctc tctggttccg gagggcgctg ctatggcttc
     4441 gctgatcgga ctgctttcct tccctgtgat gatcctggca ttttacgggg aatattccga
     4501 gtatatgggc cattccgaag taatttgtgc gctggatgaa gagatggctg taaaatggaa
     4561 acggctctgg aagtggtttg tgtattcatt tggattaatg ataagctccg ttcttctggt
     4621 gatgttaaac gggatgctgg gaattacggc gcttttggta ggtaccatcg gaacactgat
     4681 cgtaagcgtt atgaagctga cctgcctgta ccgcactgca aaaatattct gggaatatgc
     4741 aacggagagc agcagataac gggggagcaa aaaagacgaa aacgtttgca aaactctcac
     4801 gaaaatttct gttatctctg attgtattta
//
LOCUS       DBMDUH010000178         4776 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_6696_length_4776_cov_5.285989, whole genome shotgun sequence.
ACCESSION   DBMDUH010000178 DBMDUH010000000
VERSION     DBMDUH010000178.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4776)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4776
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6696_length_4776_cov_5.285989"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(247..1359)
                     /locus_tag="ACZPCG_11890"
     CDS             complement(247..1359)
                     /locus_tag="ACZPCG_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HIR1285786.1"
                     /translation="MPPISVLMKPSSGMCNMHCDYCFYCDEAQKRLQKSYGFMSDETL
                     KNVIRKTILRAEGVISYAFQGGEPTLRGLDFFKKAVAYEKQYNRNHIKVQNAFQTNGY
                     LLDDEWCAFFKENNFLVGLSLDGIRETNDIYRHAAGCGSVFERIFGAAKLLQKHGVEF
                     NILTVVTGNTAAHITEIYEFYRRNGLGYQQYIACLDPLEEPHGQNPYALSPKQYGEFL
                     ITLFHLWYKDWKRGRQPYIRQFENYIGILMGYRPEACDQCGICNIQNAVEADGSVYPC
                     DFYVLDEYKLGNFNTNQLDSIDRKRTEIGFIERSKKLKKSCLSCPYFSICRGGCQRNR
                     DLDMASGLYENYFCESYKMFFEACLPLMSEVAAAAQ"
     gene            complement(1371..2915)
                     /locus_tag="ACZPCG_11895"
     CDS             complement(1371..2915)
                     /locus_tag="ACZPCG_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR1285787.1"
                     /translation="MIKVLIADDEPLVRAGIKTVLPWNMYGFDVISEAADGKEAYEKI
                     LKLKPDILITDIKMPGMDGITLLKRLKQEKISIQSLVLSCFDEFELVREAMKYGAHDY
                     IRKLSIDPAKLLEVLKEMKEAISDQPEKNASFSLNTDDLKYLFIKRLQNQGFEDHEQV
                     KNVLHNIKLDISIQDYHLIRFSFEPDTAPITDTNRKNMIYNLLNQICERYSGQELFSL
                     DEKGYLLISNTKKDLLLCRQIGAAMKQYANQTVYFGISPLLKDETSFKAGMKQAEEAL
                     SACIFYERSEPLPYPLLTSVGFPFITDSQAQELYIALSSGNSEKSSELVQRFLLWLKS
                     GLFYPFQCYSYMEEILNIFIRVARELNFSLYQMTADENDILRRIRQAHTLRTCQKILS
                     DFIMEFSEFVRDKRSGERSEILKIKEYVQLHYSENIDLNLVAGLVNVTPSHLSNLFKK
                     ETGTNFSSYLTDVRMQAAGKLLKSPDMLIYEVAEKTGYSNGGYFGKAFKKYWGVSPEE
                     YKKEKR"
     gene            complement(2890..4605)
                     /locus_tag="ACZPCG_11900"
     CDS             complement(2890..4605)
                     /locus_tag="ACZPCG_11900"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1285788.1"
                     /translation="MIKHTFMKLNLSCRLFLIIIVLFLVPYLTLFSWVYKKAETIIRD
                     KAQSLERENLNQTKNDIESLCLNMIQASDYLISLNNYGVLYREASTKGYSYLKCWQDA
                     NEQIQNVNNSLLNSNADISVLSNDQLLYSTLPQQKFRFEDFFAEQVVSSVYFTNAHQS
                     YRRFESNQTFVSYIRKLPPFSPKSFYLVISLPASSFSRLLGAAAGNMELWDTSGYSIC
                     GIHTPKDVKYFQEEISIPLSGWRLTDTISTEFLYQDIYELRTFTFVVSLGLLMICLLT
                     TFFAIYSQLRPLFKLKEQMQQVMAGNLNAEIATTDSKDEISSLSRTFNNMVTEIGHLI
                     EEIQVTQKRESELRFEMLLAQINPHFLFNTLNSIKWMSVMSGTEHITNTITALGRLLE
                     ISMNKVNDVLPIEEELENIKSYIKIQQVRYPGRFDVAYHIEEGILKEHTLKLILQPLV
                     ENSILHNIEARDFLMIDISGRCENGIIILQVQDNGTGMDADTMKEILKPKKQGKKGYV
                     FSGLGVSNVQERIQLAYGPDYGLQYDSDGNSFTTVTIRFPQHKQIDSLLSERRENNDK
                     GTDCR"
BASE COUNT         1339 a          975 c         1054 g         1408 t
ORIGIN      
        1 tttcttttgt tttgccatag taaaaggccc tcctaatgat atttattgta tcatagaagg
       61 accttactta ggttatttta cagaaaaagt ttcataggct cgaatcagat cacattcttc
      121 tgaaagaaca ttcaatgccc ggtaagtcag ataaaataca ttagtaagtc tgaaataaag
      181 aaactctaaa tattccataa attctgaaga gaaaaggaat atttagagtt ttctttttag
      241 taacaatcat tgcgcggctg cagcgacttc actcatgagc gggaggcagg cttcgaaaaa
      301 cattttataa ctttcgcaga aataattttc ataaagccca cttgccatat ccagatcacg
      361 gtttctctgg catccaccgc gacagatgga aaaatatgga caggacaggc agctcttctt
      421 cagttttttg gagcgttcaa taaaaccgat ttccgtccgc ttcctgtcaa tcgaatccaa
      481 ctgatttgta ttaaaatttc ctagtttata ctcatccagt acatagaagt cgcagggata
      541 cacactgccg tctgcttcca cggcattttg tatgttgcag ataccgcact ggtcgcaggc
      601 ctccgggcgg tatcccatta ggatgccgat gtagttttca aactggcgga tgtacggctg
      661 gcggcccctt ttccagtctt tataccatag gtggaatagt gtgatcagaa actccccata
      721 ctgttttggt gagagggcat aaggattttg cccatgaggt tcctctaagg gatccagaca
      781 ggcgatatac tgctgatatc ctaagccgtt tctacggtaa aactcataga tttcagtgat
      841 atgtgcagcg gtatttcccg ttacaacggt aaggatatta aattctacac catgcttttg
      901 cagaagtttg gcagcgccga aaatgcgctc aaacacggat ccgcacccgg cagcatggcg
      961 gtaaatatca ttggtttctc tgataccgtc taaggaaagc ccaactaaaa agttattttc
     1021 cttgaaaaat gcacaccatt catcatccaa aagataacca tttgtctgaa aagcattctg
     1081 caccttgatg tgattgcggt tatactgttt ttcataggca accgccttct tgaaaaaatc
     1141 gagtccccga agggtaggtt cgccaccctg aaaagcatag gaaataacgc cttccgcccg
     1201 caggatggtc tttcgtatta catttttgag tgtctcatcc gacatgaacc cataggactt
     1261 ctgcagacgt ttctgggcct catcacaata aaagcaatag tcgcagtgca tgttgcacat
     1321 gccggatgat ggtttcataa gcacgctgat tggtggcatg ataaaaactc ctaacgtttt
     1381 tcttttttat attcttccgg ggagacgccc cagtattttt taaaagcttt tccgaagtat
     1441 ccgccattgg aatatcccgt cttttcagca acttcataaa tgagcatgtc cggggatttt
     1501 aaaagtttgc cagctgcctg catccgcaca tcggtgaggt aggaagagaa gttggtgccg
     1561 gtttctttct taaaaagatt gcttaaatgg gatggagtga cattcacgag accggcaacc
     1621 aggtttaagt cgatattttc gctatagtga agttggacat attcttttat cttcaaaatt
     1681 tcagacctct ctccggaacg tttatcgcgg acaaattctg aaaactccat aataaaatca
     1741 gacaggattt tctggcaggt ccgtaaggta tgggcctggc ggatgcgcct tagaatgtca
     1801 ttttcatccg ctgtcatctg gtaaagggaa aaattcagtt ccctggccac ccggatgaaa
     1861 atgttcagga tctcttccat ataagagtaa cactgaaaag gatagaatag gccgctttta
     1921 agccatagta aaaagcgctg gaccagttcc gaagattttt cactgtttcc ggaggaaagg
     1981 gcaatataaa gttcctgggc ctgtgaatcc gtaatgaatg gaaagcctac ggaggtaagc
     2041 agcggataag gcagcggttc acttcgctca taaaaaatgc aggcagaaag ggcttcttcg
     2101 gcctgtttca ttccggcttt aaaagaagtt tcatccttta ataaaggact gataccgaaa
     2161 tatacggtct gattggcgta ttgtttcatg gcagctccga tctggcggca gaggagaaga
     2221 tctttctttg tgttggatat aagaaggtat cctttttcat caagggaaaa cagttcctgt
     2281 cctgagtaac gttcgcagat ctggtttaac aggttataga tcatgttttt gcgattggta
     2341 tccgtaatcg gagcagtatc tggttcaaag gaaaaacgga tcaggtgata atcctgaatg
     2401 gaaatatcca gtttgatgtt atggagtaca ttcttcacct gctcgtgatc ctcaaacccc
     2461 tgattttgaa ggcgcttgat aaaaagatat ttcagatcgt ctgtatttaa agaaaaggaa
     2521 gcgttctttt ccggctggtc agagatggcc tctttcattt cctttaagac ttccagtagt
     2581 ttggccggat caatagaaag ctttcggata taatcatggg caccgtactt catagcttca
     2641 cggacaagtt caaactcatc aaagcagctt aggactagag actggatgga tattttttcc
     2701 tgtttcaggc gtttgagtaa agttattccg tccattcccg gcattttgat gtcggtgatc
     2761 agaatgtcgg gtttgagttt taagattttt tcgtaagctt ctttgccatc ggccgcttct
     2821 gagatcacat cgaaaccata catattccat ggaaggacgg ttttgatgcc ggcccttacc
     2881 aatggttcat catctgcaat cagtaccttt atcattgttc tctctccttt cggacagaag
     2941 gctgtctatc tgtttatgtt gcgggaaccg gatcgtcacg gttgtaaaag agttgccgtc
     3001 gctgtcatat tgtaagccgt aatcaggacc ataagccagc tgtatgcgtt cctggacatt
     3061 gctcacgccg agaccggaga agacataccc ctttttaccc tgctttttgg gttttaggat
     3121 ttctttcata gtatctgcat ccatgcctgt tccattatcc tgaacctgca ggatgataat
     3181 gccgttttca caccggccgg aaatatcaat catgagaaaa tcccgtgctt caatattgtg
     3241 gagaatggaa ttttccacca ggggctgtag gatcagtttt aaggtgtgct cttttaaaat
     3301 tccctcctct atgtggtagg ccacatcgaa acggccggga tagcgcacct gctggatctt
     3361 gatataactt ttgatgtttt ccaactcttc ttcaatagga agcacgtcat tgaccttatt
     3421 catgctgatc tcaagaagcc ggccaagagc ggtaatggtg ttggtgatgt gttctgtccc
     3481 ggacattacg gacatccatt tgatggagtt tagggtatta aagagaaaat ggggattgat
     3541 ctgggccaga agcatttcaa agcgcagctc actttcccgt ttctgagtga cctgtatttc
     3601 ctcaatcaga tggccaatct ctgtaaccat gttgttgaag gttcgggaaa gactgctgat
     3661 ctcatctttg gaatcggtag tggcgatttc ggcattcagg ttcccagcca taacctgctg
     3721 catctgttct ttcagcttaa aaagtggacg cagctgcgaa tagatagcga aaaatgtggt
     3781 cagaagacat atcattaata gtcccaggga tacaacaaac gtgaatgtgc gcagttcata
     3841 gatatcctga tagagaaatt ccgtagaaat ggtatccgtc aggcgccagc ctgaaagtgg
     3901 aatggagatt tcttcctgga aatatttaac atctttggga gtatggatcc cgcagatgga
     3961 ataaccggaa gtatcccata attccatgtt ccctgctgca gctcctaaca gacgggaaaa
     4021 actgctggca ggaagggaga tgaccagata aaagcttttc ggtgaaaatg gcgggagctt
     4081 tctaatatag gaaacgaaag tttgattgct ttcaaaacgg cggtaactct ggtgggcgtt
     4141 cgtaaaatat accgacgaaa ctacctgctc cgcaaaaaaa tcctcaaagc ggaatttctg
     4201 ctggggaagc gtggagtaga gcagttggtc attggataaa acactgatgt cagcattgga
     4261 atttaacagg gaattattta cattctggat ctgctcattg gcatcctgcc agcattttaa
     4321 ataggaatat ccctttgttg aggcttcacg gtaaagaaca ccgtaattat ttaaggatat
     4381 cagataatca gaagcctgaa tcatgtttag gcagagactc tcaatgtcat tttttgtttg
     4441 gttcagattt tctcgttcca gtgactgagc cttgtctcgg ataatggttt cagctttttt
     4501 gtacacccat gaaaataggg tcagataagg aacgaggaac agaacaataa taataaggaa
     4561 taaacggcag gacaggttta gtttcataaa agtatgtttt atcataataa atcctccctc
     4621 ttccttatat tgttgagatt ttaaagcggt atcttgtgtt taaagtatac aatagtgaaa
     4681 gttcaaaaaa aagtatcttt gaaaattgcg taaaaaagta ccatttttca aaaaaatatt
     4741 accacaagcg taaaaaaata ccaatttgcc ttttta
//
LOCUS       DBMDUH010000179         4762 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_6721_length_4762_cov_7.925767, whole genome shotgun sequence.
ACCESSION   DBMDUH010000179 DBMDUH010000000
VERSION     DBMDUH010000179.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 4762)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4762)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4762
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6721_length_4762_cov_7.925767"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1054)
                     /locus_tag="ACZPCG_11905"
     CDS             complement(<1..1054)
                     /locus_tag="ACZPCG_11905"
                     /inference="COORDINATES: protein motif:HMM:NF015445.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HIR1285789.1"
                     /translation="MVNRKKRLFFLTAAAVLCLIAASCGSASDGFDGRMAEPSITLLL
                     KNNLPDESITTKAVEWFSEQVEERTEGRVKIEVLNNADMGDGRACLEQLQYGGTDIVK
                     ADVSALGNFVEEFNVLGMPYIYEDPEHFWAVHNGPIGEELLRGAQMAELGMYGLTYYD
                     GGSHCFYSSRGEIHSPGDLEGLDVRVQQSETALSMARALGARPVTADYSELYRTLQSK
                     EADAAENSIVNYYAQAFYEVAPYFTEDNHTRSADVLVMSLESRKKLSEGDLLILDKTA
                     EESCEYQRKLWAEEEAAVREALLQKNAVITQLTDSEYEAFRQVCEQIWYSYRDGRYID
                     LIDRIVAVGYLQKLPEQ"
     gene            complement(1095..1925)
                     /locus_tag="ACZPCG_11910"
     CDS             complement(1095..1925)
                     /locus_tag="ACZPCG_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HIR1285790.1"
                     /translation="MRKKQVVLMNFSGIYETESFYETVPQENRIWLDCRTIPGTNCYC
                     DEEGERELKERMEPLGAEGIHFLDSGNYHYVTKLWLDQVKEPFDLLVLDHHTDLQQPM
                     FGDILSCGGWIRASLEENPYLNRVFLAGPPCEAVKEAENELPEELWKKVVWIPEEALK
                     EKWGAGVLKYARCFGDSGCAGDIRRLEDSEDPVYLSIDKDVLSPDFARTNWDQGQAGI
                     AETADFIRTFLKGRTLIGADICGENPEGAEGPEAEEERRINDTANGLLLETVLGCFDE
                     "
     gene            complement(1943..2500)
                     /locus_tag="ACZPCG_11915"
     CDS             complement(1943..2500)
                     /locus_tag="ACZPCG_11915"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003526032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HIR1285791.1"
                     /translation="MIYQTVYESPVGALTLTASEDALTALSFPQERYPEDVERLTKER
                     EVCIPAADKIPRILRETREWLDIYFSGKRPDFTPALAPQGSDFRKVVWEELLKIPYGC
                     TITYGQIAKQVADRKGILRMSAQAVGGAVGHNPIGIIIPCHRVVGTGGNLTGFGGGIR
                     TKEALLKLEGMDMGRFYIPKKGTAL"
     gene            complement(2671..3039)
                     /locus_tag="ACZPCG_11920"
     CDS             complement(2671..3039)
                     /locus_tag="ACZPCG_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR1285792.1"
                     /translation="MNVIDTKNAPGAIGPYSQAFEVNGVIYTSGQIPVNPADGTVADG
                     IAAQAEQSCKNVGAILEAAGSGFDKVFKTTCFLADMGDFAAFNEVYAKYFTSKPARSC
                     VAVKTLPKGVLCEIEAIAVK"
     gene            complement(3129..4445)
                     /locus_tag="ACZPCG_11925"
     CDS             complement(3129..4445)
                     /locus_tag="ACZPCG_11925"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HIR1285793.1"
                     /translation="MKIQIPAAAQEIVEKLCEHGYEAYLVGGCVRDMLLGREPGDWDI
                     TTSAKPEEVKAVFRRTVDTGIKHGTVTVMMGKEGYEVTTFRIDGDYTDGRHPDSVTFT
                     PDLIEDLKRRDFTINAMAYNRDRGLVDAFGGRKDLEDGVIRCVGAAEERFTEDALRIL
                     RAIRFSAQLGFEIEEHTENAIKKIAPNLVHVSRERVQVELTKLLISPLPGRIRQVYET
                     GISPWVSETFHRIPEISGGSCFHIPSSVPAEKSLRWAVFMRKCEPKEAVLVLKALKLD
                     NDTIYRVKTLVQWIKVSLCGEPTEDSIQSDKDGAAIQAGYLKKELSKHNDRIKTGLRR
                     AMSRMEPELFDDLLRVKLCLAEECPPAAECLRALCCLRDEIRRDGDCISLKMLAVTGN
                     DLIKAGMKPGKEVGTRLQQFLELVLEDPVRNTKEYLLSLLSSVSDA"
     gene            complement(4445..>4762)
                     /locus_tag="ACZPCG_11930"
     CDS             complement(4445..>4762)
                     /locus_tag="ACZPCG_11930"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566114.1"
                     /note="catalyzes the formation of prolyl-tRNA(Pro) from
                     proline and tRNA(Pro); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HIR1285794.1"
                     /translation="CLDELEAKAGELLETIQKDMLEKARAHREAHTYTATEWDEFVDT
                     INNKPGFVKAMWCGCRECEDKIKEETGATSRCMPFEQENLSDHCVCCGKPAKKMVYWG
                     RAY"
BASE COUNT         1089 a         1451 c          945 g         1277 t
ORIGIN      
        1 cctgctcggg cagcttctgc agatatccaa ctgccacgat ccggtcgatc aggtcaatat
       61 acctgccatc ccggtagctg taccagatct gctcacaaac ctgacggaat gcttcatatt
      121 cactgtctgt cagctgagtg atcacagcat ttttctgtaa aagcgcttct ctcacagccg
      181 cctcttcctc agcccacagc ttcctctgat attcgcagga ttcctccgcc gtcttatcca
      241 gaatcagcaa atctccttcg gacagctttt tccggctttc caggctcatc accagcacat
      301 ctgcagaccg tgtgtgattg tcctctgtaa aataaggcgc cacctcataa aatgcctgag
      361 cataataatt tacaatggaa ttttccgccg cgtctgcctc ctttgactgc agcgtacggt
      421 acagttcgga ataatccgca gttaccggcc ttgcccccag tgctcttgcc atagaaagcg
      481 ctgtttccga ctgctgaacc cgtacgtcaa gtccctcaag atctccagga ctgtgtattt
      541 cccccctgga actgtaaaag cagtggcttc ctccgtcata gtaggtcaat ccgtacatcc
      601 caagctctgc catctgcgct ccccggagca gttcttcacc gatcgggcca ttatgaaccg
      661 cccagaaatg ttccggatcc tcataaatat acggcattcc cagtacatta aattcctcaa
      721 caaaatttcc cagagccgaa acatctgcct ttacgatatc tgttcctccg tactgaagct
      781 gctctaaaca ggcgcgcccg tcccccatat ccgcattatt caatacttct atttttaccc
      841 gtccctccgt ccgctcctct acctgctccg aaaaccattc caccgctttt gttgtaatgc
      901 tttcatctgg aagattattc tttaaaagaa gcgttatcga aggctctgcc atccgaccgt
      961 caaagccatc ggaagcagaa ccgcaggatg ccgctatcaa acaaagtaca gcggcagcag
     1021 tcagaaaaaa cagccgtttt tttctattca ccatgatttt ttccttttgt ttatcttatc
     1081 gcagcagcca tcatttattc atcaaagcag ccaagaaccg tctctaaaag cagcccattg
     1141 gcagtatcat tgatccgccg ctcctcctct gcctccggcc cctctgcgcc ttccgggttt
     1201 tcaccgcata tatctgctcc gatcagtgta cgtcctttaa gaaaagtcct gataaaatcc
     1261 gccgtttccg ctatgcctgc ctgtccctga tcccagttgg tgcgggcaaa atcgggcgaa
     1321 aggacatcct tatcaatgga gagatacaca ggatcctcag aatcctccaa tcgccggata
     1381 tctccggcgc atccagaatc cccaaagcat ctggcatatt ttaaaactcc ggctccccac
     1441 ttctccttaa gtgcctcctc cggaatccat accacctttt tccacagttc ttccggaagc
     1501 tcattttccg cttctttcac tgcctcacag ggtgggcctg ccagaaaaac ccggttcaga
     1561 taaggattct cctccaaaga ggcccgaatc catcccccgc aggagagaat atctccaaac
     1621 atgggctgct gcagatctgt gtgatgatcc agtaccagaa gatcgaaggg ttccttcacc
     1681 tgatccagcc acagctttgt gacataatga taattgccgg aatccagaaa atggatcccc
     1741 tccgctccaa gaggctccat cctttccttc agctcccttt ccccctcttc gtcacagtag
     1801 cagttggtgc cgggaattgt tctgcagtcc agccatattc tgttttcctg cggaaccgtt
     1861 tcataaaagc tctctgtttc gtagatcccg ctgaaattca tcagaaccac ctgttttttt
     1921 ctcatttgtt tctggctcct ttttacagtg ctgttccctt cttcggaata taaaaccggc
     1981 ccatatccat gccctccagt ttaagcagtg cttcctttgt ccgaatcccg cctccaaatc
     2041 cggttagatt tcctcccgtt cctacaaccc ggtgacaggg aatgatgatg cctatgggat
     2101 tatgtcctac cgctcctccc actgcctggg cagacatccg caaaatgcct tttcggtctg
     2161 ccacctgctt tgcaatctgg ccataagtta ttgtacagcc ataggggatt tttaacagtt
     2221 cctcccagac cacctttctg aaatcacttc cctgcggcgc cagagccggg gtaaagtccg
     2281 gcctttttcc tgaaaagtaa atatccagcc attcccttgt ttctctcaga attcggggga
     2341 ttttgtccgc agcaggaata catacctcac gctctttcgt cagccgctct acatcttcgg
     2401 gataccgctc ttgtggaaag gaaagcgccg tcaaagcatc ctctgaagct gtaagagtca
     2461 aagcacctac aggggactca tacactgtct gataaatgat tcttcctttg tcatccatcc
     2521 gctcaaattc tcctgctctc ccagatcata ccgtttttcg tgccgctccc gccatctgtc
     2581 cgatcaatcc cggcccagag tccggtccgc cttcaggcaa agaaaaagga gccctgcggc
     2641 tcccttccct tcttatcacc tgcaggcaga ttactttact gcgattgcct cgatctcaca
     2701 taaaactccc tttggaagag tctttactgc tacgcagctt cttgccggct tggaggtaaa
     2761 atactttgca tatacttcat taaatgctgc aaagtcgccc atatctgcta aaaagcaggt
     2821 ggtcttgaat accttatcga agccgctgcc tgctgcctcc aggatagcgc ctacgttctt
     2881 acagctctgc tctgcctgag cggcaatgcc gtctgctact gtaccgtctg ccggatttac
     2941 aggaatctgt ccggaagtat agattacccc atttacctca aatgcctgag aataaggtcc
     3001 gattgcacct ggtgcgttct ttgtgtcaat tacgttcatt ccgaaaacct cctaatacct
     3061 taataaggcc tattatagcc gacttttata cattatgcaa tgttttattc ttaatgcaga
     3121 tcctatgttt atgcatcact tacagaggac aacagagaca gcagatattc cttcgtattc
     3181 ctcaccggat cctccagcac cagttccaga aactgctgca gccgggttcc aacctctttt
     3241 ccgggcttca tccccgcctt tataagatca ttccccgtaa ctgccagcat tttaaggctt
     3301 atgcagtcgc catcccggcg gatctcatct ctgagacagc aaagtgctct caggcactcc
     3361 gccgcaggcg gacactcctc agccagacac agctttactc taaggagatc gtcaaacagc
     3421 tccggctcca tccggctcat agcccggcgc agtccggttt ttatcctgtc attatgcttg
     3481 gaaagctctt tttttaaata accagcctgt atagctgcac cgtctttatc ggactgtata
     3541 ctgtcctctg tcggctcccc gcacagggaa actttaatcc actgaaccag agtctttacc
     3601 ctgtaaatag tatcattatc cagcttcagc gccttaagca ccaaaacagc ctctttaggc
     3661 tcgcatttcc gcataaaaac cgcccagcgc agtgattttt ccgccggaac agaggacggt
     3721 atgtgaaagc aggagccgcc ggaaatctcc gggatccggt gaaaggtctc tgatacccag
     3781 gggctgatcc ctgtctcgta aacctgccgg atgcgtccgg gaagggggct tataagaagc
     3841 tttgtaagct ccacctgaac ccgctcccgg cttacatgga ccagattggg agcaattttt
     3901 tttatggcat tttccgtatg ctcctcaatt tcaaacccaa gctgggcgga aaaacggatg
     3961 gctcttaaaa tgcgcagagc gtcctctgta aagcgctcct ctgccgctcc cacacagcgg
     4021 atcacaccat cctccagatc cttccggcct ccaaaggcat ccacaagacc gcgatcccgg
     4081 ttataagcca tagcattgat ggtaaaatcc cggcgcttta agtcctcaat gagatccggc
     4141 gtaaaggtca cgctgtccgg atgacggcca tcagtataat cgccgtcaat ccggaaggta
     4201 gtaacctcgt agccttcctt tcccatcatt acagtcaccg tgccgtgctt gattcctgta
     4261 tccacggttc tgcggaatac agccttgacc tcctccggtt ttgcactggt ggtaatatcc
     4321 cagtctcccg gctcccgtcc cagaagcata tcccgtacgc agccgcctac cagatacgcc
     4381 tcatatccat gctcacaaag cttttctaca atctcctgtg cagcagcggg aatctgtatt
     4441 ttcatcaata tgcccgtccc cagtaaacca tctttttcgc cggcttgccg cagcatacgc
     4501 agtggtcgga gagattctcc tgctcaaagg gcatacatct ggaggtagcg cctgtctctt
     4561 ccttaatctt atcctcacac tcacggcagc cgcaccacat agcctttaca aaacctggct
     4621 tattgttaat ggtatccaca aactcatccc attctgtagc agtataggta tgagcctctc
     4681 tgtgagctct ggccttttcc agcatatcct tctgaatggt ttcaagcagt tcgccggcct
     4741 ttgcctccag ctcgtccagg ca
//
LOCUS       DBMDUH010000180         4638 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_6902_length_4638_cov_6.329072, whole genome shotgun sequence.
ACCESSION   DBMDUH010000180 DBMDUH010000000
VERSION     DBMDUH010000180.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 4638)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4638)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4638
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6902_length_4638_cov_6.329072"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            125..964
                     /locus_tag="ACZPCG_11935"
     CDS             125..964
                     /locus_tag="ACZPCG_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016441306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR1285795.1"
                     /translation="MKMQKMKHEITEIAPNTWCLSEFRLVNAFLAEGKEKAALIDTGC
                     GIGNLGETVKELTQKPVEIFLTHGHADHSGGIYTLPQSPVYMMKEDEKLFQEMPATNE
                     MRRFYVKTRVPVRYPGEGNVEAITALIPEQEPEYTGEYTAVKDGDIFELGERTLTALH
                     TPGHSEGSVCYLDSQSRVLFSGDTVNHSIILMRQPDNDKRLIRIYHESLKKIWEQQEK
                     FDRLAIGHDGILLDKAIVKDYLELTGGLLDGSIVGKYEEIGFRKGDVARLGQAELWYQ
                     CDE"
     gene            complement(1123..1404)
                     /locus_tag="ACZPCG_11940"
     CDS             complement(1123..1404)
                     /locus_tag="ACZPCG_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009219357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000987 - cis-regulatory region
                     sequence-specific DNA binding [Evidence IEA];
                     GO_function: GO:0015643 - toxic substance binding
                     [Evidence IEA];
                     GO_process: GO:0044010 - single-species biofilm formation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HIR1285796.1"
                     /translation="MATTNINVRIDTELKQSAEALFADLGLNMSSAITMFLKSAVSHD
                     GIPFEVKRITPNAETRAALAEYGEMKKHPDKYKRYSSFEDLMDEVLADA"
     gene            complement(1495..3333)
                     /locus_tag="ACZPCG_11945"
     CDS             complement(1495..3333)
                     /locus_tag="ACZPCG_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002605670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HIR1285797.1"
                     /translation="MTLSTHTKKHLWLQTLLALLLSISLLTGCAAPAAPAPSEASSQA
                     EAAASAYEQYDEDTLKVQSDFDAFTDQLFCSEVAQSPITLHYTLADPSSYGITDYPRD
                     LGTVSVEDIKKDLTDVRELYSRLTALDSRKLREDQLLTYTILSSYLNTCLSLEGLELY
                     DQPLSATLGIQSQLPILLSEYQFYTRQDVEDYLSLLSSIKTYYDSIIAFEQQKSEASL
                     GLCDAVIDRIIPSCSAYLIDADHSFMAETFASRLNRIDGLTEQEKNDYISRNKAALNE
                     HFVPAYESLIEGLKTLKGKGTNGGGLCHFPEGKRYYEYLVNAQTGTSYKDIPSLKKAV
                     MDQMADDLTAVDELLTADPSLSEKILSYRFSPEKPEEILEALKTQCQQDFPELTGYSY
                     TIKSVPKPLESILSPAFYLTVPLDRPQDNSIYINNGSTGSSSLYTTIAHEGHPGHMYQ
                     NQYFNQNQHCNLRSILDFKGYSEGWATYVEHYAYTLENNGLEEGVGELLHHNAAFTLA
                     LYAILDISIHYEGWDMEQVKNYLDLYFKITDPDVVSSIYYEIMENPANYLTYYTGYLE
                     ILTMEREARQTLGANYSQLEFNRFLLDMGPAPFGVIRTYFVKWLVK"
     gene            3617..>4638
                     /locus_tag="ACZPCG_11950"
     CDS             3617..>4638
                     /locus_tag="ACZPCG_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR1285798.1"
                     /translation="MCQDCKKPYYITTAIAYASGKPHIGNTYEIVLADSIARYKRAKG
                     YDVFFQTGTDEHGQKIEEKAEAAGVTPQEFVDRSAGEIRRIWDLMNTSYDKFIRTTDK
                     DHEAQVQKIFKKLYDQGDIYKGAYEGLYCTPCESFWTPSQLVDGKCPDCGREVQPAKE
                     EAYFFRMSKYAPRLIEYINEHPEFIQPVSRKNEMMNNFLLPGLQDLCVSRTTFSWGIP
                     VDFDPKHVTYVWLDALTNYITGIGYDCDGNHTDQFKKYWPADLHLIGKDIIRFHTIYW
                     PIFLMALDLPLPKQVFGHPWLLQGDGKMSKSKGNVLYADTLVDFFGVDAVRYFVLHEM
                     PFENDGV"
BASE COUNT         1263 a          959 c         1206 g         1210 t
ORIGIN      
        1 atgattggcg ctcttctttc atttgtctgc tttaaattta tttacaatgt aagcgatgca
       61 cgaatgagag aaatcagcga ggaacttcgc agaaaaagcg aggagacaga ggagagaatt
      121 ttaaatgaaa atgcagaaaa tgaagcatga aataactgag attgcgccca atacctggtg
      181 tctgagtgag ttcaggctgg tgaatgcctt cttggcagaa ggaaaagaaa aggccgcttt
      241 aattgatacc ggctgcggga ttggaaatct gggagagaca gtaaaagagc tgacgcagaa
      301 gccggttgaa atttttctga cccatggtca tgcagatcac agcggaggga tctataccct
      361 tccccaaagc ccggtatata tgatgaaaga ggatgaaaaa ctgtttcagg aaatgcctgc
      421 tacgaatgag atgcgccggt tttatgtaaa gacccgggtg ccggtccgtt atccgggtga
      481 aggcaacgtg gaagcgataa ccgctctgat acctgagcag gaaccggaat atacagggga
      541 atatacggct gttaaggatg gggatatttt tgagctgggg gagagaacat taacagcatt
      601 acatacgccg ggacattcag aaggaagcgt atgctatctg gacagtcaaa gccgcgtgtt
      661 attcagcgga gatacggtga accatagcat tattttaatg cgtcagcctg ataatgacaa
      721 acgtctgatt cggatttatc atgaaagcct gaagaaaatc tgggaacagc aggaaaaatt
      781 tgacagactt gcaatcggac atgacggaat ccttttagat aaggcaatag taaaagatta
      841 tctggaactg acgggaggac ttctggatgg aagcattgtg ggaaaatatg aagagatagg
      901 attcagaaag ggagatgttg cacgtttggg acaggcagaa ctgtggtatc agtgtgatga
      961 ataagagggg gtaaacgcaa aagggagcgg tttaaggaat cgctcccttt tgactgcttc
     1021 tgtgctgcca gagtattaat aactgcggtg aggcattcca gtttaagccc tcgttttttg
     1081 gcgagtttca gatctcttct gaattggttg gagggaacaa tgttaagcat ctgcaagtac
     1141 ctcatccatt aaatcctcaa aagatgagta tctcttatat ttgtccggat gtttcttcat
     1201 ctcaccatac tctgcaagtg ccgctctggt ttcagcattt ggtgttatcc gctttacttc
     1261 aaaaggtata ccatcgtggc tgacagcgct tttaagaaac atagtaattg cagatgacat
     1321 attaagtccg agatccgcaa acagtgcttc agcagactgt tttagttctg tatcaatacg
     1381 aacattgata ttagttgtag ccatgaaaat tacctccttt catttaactt tagtatatgc
     1441 aatgatgcaa acattgtcaa taaaatgttt tacattgttt tgcatacata taatttactt
     1501 caccagccac ttcacaaaat acgtccggat taccccaaaa ggcgccggcc ccatatccag
     1561 aagaaagcgg ttaaattcca gctgggagta atttgcgcca agtgtctgtc tggcctcccg
     1621 ttccatggtg aggatttcca gataacctgt atagtaggta aggtaattgg cgggattctc
     1681 cattatttca taatagatag aggataccac atcagggtct gtgatcttaa agtaaagatc
     1741 cagataattc tttacctgct ccatatccca gccctcatag tggatgctga tatccagaat
     1801 ggcgtaaaga gccagagtaa aggccgcatt gtgatgcagc agttctccta cgccttcctc
     1861 cagaccatta ttttccagag tataggcata gtgctccaca taggtagccc atccctcgga
     1921 atagccctta aaatcaagga tacttcttaa attacagtgc tgattctggt taaaatactg
     1981 gttctgatac atatggcccg gatggccctc atgggcaata gtggtgtaga ggctggagct
     2041 tccggtggaa ccgttgttga tgtaaatgga attgtcctga ggacggtcta agggcacagt
     2101 gagataaaag gccggactta aaatactttc cagaggcttg ggaacagact taatggtgta
     2161 ggaatagccg gttaattcgg gaaaatcctg ctggcactga gtttttaagg cttccaggat
     2221 ctcttcgggc ttttcgggag aaaagcggta ggaaaggatt ttttcactca gagagggatc
     2281 tgctgtgagc agctcatcga ctgcggtgag atcatccgcc atttgatcca tcactgcctt
     2341 tttcagggaa gggatatcct tataggacgt gccggtctgg gcgtttacca ggtattcata
     2401 gtacctcttt ccttcgggaa aatgacacag gccgccgcca ttggttccct ttcccttcag
     2461 ggtttttaag ccctcgatca ggctttcgta ggcaggcacg aaatgctcgt tcagagcagc
     2521 tttatttcgg gagatatagt catttttttc ctgctctgta aggccgtcga tcctatttaa
     2581 acgggaagca aaggtttctg ccataaagct gtgatctgcg tcgatcagat aggcgctgca
     2641 ggatggaatg atccggtcga tgactgcgtc gcacagtccc aggctggcct cggatttctg
     2701 ctgttcaaag gcaatgatgc tgtcgtagta ggtttttatg gacgataaaa gagacaggta
     2761 gtcttctaca tcctgacggg tataaaactg gtattcagat aagagaatgg gaagctggga
     2821 ttgtatgccc agagtagcag agagcggctg gtcatagagt tccagtccct ccagagacag
     2881 gcaggtattt aagtatgatg aaagaatggt ataggtaaga agctggtcct cgcgcagctt
     2941 ccggctgtcc agcgcagtca ggcggctgta cagctccctg acatcggtca ggtctttttt
     3001 tatgtcctca acagaaacag tccccagatc tctgggatag tcggtgatgc cgtatgagga
     3061 tggatctgcc agagtgtagt gaagcgtaat cggggactgg gcgacttcag agcagaagag
     3121 ctggtccgta aaggcgtcga agtcactttg gacttttaag gtgtcctcgt catactgttc
     3181 ataggctgag gcagcggctt ccgcctgaga ggaagcttca gacggggcgg gcgcagcggg
     3241 agcggcgcag ccggtcagca gggaaatgga caggagcagg gctaaaagag tctgcagcca
     3301 taaatgtttt tttgtatggg ttgatagggt catgtgttat attcctccga aatttgataa
     3361 ggtatgtttt tgcgggatat tcatccgatg tgaactcagg tacaaaggcg ggcttacaag
     3421 cgggcttgac gcccgacttt gtacctgagt tctcgccatc ctgaattgta gtatatatta
     3481 tacgagcgga aaaaacaact tatgaggggc gctgtcttga cgaaaatgtg ggaaacgctt
     3541 atacttaaac cattaaaacg ggcaggagcg ccctgcgctt tgaatgaccc gaatccagaa
     3601 aaaagaggaa aataaaatgt gtcaggactg taaaaaacca tattatatta caacagccat
     3661 tgcatatgcc tccggtaagc cccatatcgg caatacctat gagatcgtgc tggctgacag
     3721 tatcgcaaga tataaaagag caaagggcta tgatgtattc ttccagaccg gtacggacga
     3781 gcatggacag aagatcgagg agaaggcaga ggctgcaggc gttacgcctc aggagtttgt
     3841 agaccgctcc gcaggagaga tccgccggat ctgggatctg atgaatacct cctatgacaa
     3901 gtttatccgc accacagata aggatcacga ggctcaggtt cagaagattt ttaaaaagct
     3961 ttatgatcag ggcgatatct acaagggcgc ttatgagggc ctttactgta ctccctgcga
     4021 gtccttctgg accccgtccc agctggtgga tggaaaatgt cccgactgcg gccgtgaggt
     4081 gcagcctgcc aaggaggagg cttatttctt ccgtatgagc aaatatgctc caaggctcat
     4141 tgaatatatc aacgagcatc cggagtttat ccagccggta tcccgcaaaa atgagatgat
     4201 gaacaacttc ctgcttccgg gcttacagga tctgtgcgta tccagaacca cattttcatg
     4261 gggtatcccg gtagactttg acccaaagca cgttacctat gtatggctgg acgctcttac
     4321 caactatatt acgggaatcg gttatgattg tgacggaaat catacagatc agtttaagaa
     4381 atactggcct gcagaccttc atctgatcgg taaggacatt atccgtttcc ataccattta
     4441 ctggcctatt ttcctgatgg ctctggatct gcccctgcca aagcaggtgt tcggccatcc
     4501 gtggcttctt cagggcgacg gcaagatgag caagtctaag ggaaatgtgc tttatgcaga
     4561 tactctggtg gatttcttcg gcgtagacgc tgtgcgctac tttgtgcttc atgagatgcc
     4621 atttgaaaat gacggcgt
//
LOCUS       DBMDUH010000181         4584 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_6999_length_4584_cov_6.517511, whole genome shotgun sequence.
ACCESSION   DBMDUH010000181 DBMDUH010000000
VERSION     DBMDUH010000181.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 4584)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4584)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4584
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6999_length_4584_cov_6.517511"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            56..919
                     /locus_tag="ACZPCG_11955"
     CDS             56..919
                     /locus_tag="ACZPCG_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005342602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1285799.1"
                     /translation="MTAVLKHELRNYFHTLTAYVFGAFLLAFIGLGATLYNLQAAVSN
                     FEFVLSFGSLVFVVIVPILTMRVIAEEKKQRTDQLLYSLPITTTEVVLGKYLALLVVY
                     LIPLAVVSVYPLIFARYGDVYLLTSYGSIFAFFVLGAALIALGVFISSLTDNQGFAAG
                     IGIAVILLNYYSASLSEYVSSTPAGALIAAFALVIVLGVVIRHLTKNEHLAYGFYFLA
                     GGAVLILYLADPEAFSGLLPSVMKTLSLFERFYVFVNGVFDLTAIVYFVTFAAFFLFL
                     SVQSLEKRRYN"
     gene            919..2337
                     /locus_tag="ACZPCG_11960"
     CDS             919..2337
                     /locus_tag="ACZPCG_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005331026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HIR1285800.1"
                     /translation="MKQNRNAFCGGAYSLILSAVVLTILIVVNILVNALPENLTKYDI
                     SAAKLYSITSNTKAVVNGLDEDVTIYWIVQADKEDAVIENLLNKYDSLSDHIQIVKKN
                     PDVYPTFAEQYTDEEAANNSLVVECGERSRFIGYDDIYVQEADIYSYSYNTSFDGEGA
                     ITSAIDYVVNEEQPKVYLLEGHGERELPASFKEQIEKENIQLEQLSLLNVDSIPEDAA
                     GIMIYAPSSDISGEEQTMLADYTKKGGKLFAAAGPVKEGNLDNLHGLLEEYGVTAQDG
                     IVVEGDRERYALQTPYALLPQMESSDITDALIEEHYYPILPIVQGLKVDETKAAGRVT
                     TLLSTTDQAFSKMAGYELSTYEREEGDVDGPFAAAVSISCENEGRILWFGSSDFLEDM
                     YNALSSGANGDLGMNGLSSMIGESEAMAIRSKSLNYNYLTISESASAMLQAVMIGVFP
                     LAYLGIGICVVMRRRRMQNEAG"
     gene            2324..3754
                     /locus_tag="ACZPCG_11965"
     CDS             2324..3754
                     /locus_tag="ACZPCG_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009298926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HIR1285801.1"
                     /translation="MKRAKRLYILLGVLAVLSVAAIAVIQREEHKEKIKDSGETILQI
                     PYEGVTALSWENESESLSFHKGEDGVWTYDEDEKFPVSEEKIKELLEPFEEFAAAFVI
                     EEVEDYSQYGLNDPVCTIHLENGEQELEIKLGNFSNMDSQRYVSIGDGKAYLVKDDPL
                     NYFDAVLKDMLKQDEIPELETVSSLRFTGPETYEAVRIEDSGASYSSEDVYFKKDEEN
                     LLPLDPVQVDEYIRTLQNLDLSDYASYYVSEEEWSTYGLDAPELSLEADYTFENEDKE
                     NVSGTLTVSVSRDPKEKEKAEKKENFEENSGEEEEITAYARVNNSQIAYKLTAEDYKG
                     LMAMKYDDLRHKEVFWGDTEEITGIDISLEGADYSLTSKGKKDDRTWTYQEEEIEADE
                     LLFALEGLKADRFTEEEPGQKEEIRLTLHLDNEVSPQVTIVLYRYDGSSCLAEADGKT
                     VSLIPRSQAVDLIEAVNTIVLGNTED"
     gene            3899..>4584
                     /locus_tag="ACZPCG_11970"
     CDS             3899..>4584
                     /locus_tag="ACZPCG_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002593968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HIR1285802.1"
                     /translation="MDRLGLICRNILEEPYITQRELAKRMELSLGTINGLIKECTEKG
                     YIREDEDQRGKRLALTETGQAVLAPYKVDGALIIAAGFGSRFVPLTFETPKGLLEVFG
                     ERMIERQIRQLHEAGVYDITIAVGYLKEKFEYLIDKYDVKLLYNPEYSCKNTLATVYR
                     ARHLLKGRNMYILSSDNWLRDNMYHAYECGAWYSASFQKGDTKEWCFTFTKKGRITDV
                     NVGGRDQWVM"
BASE COUNT         1273 a          972 c         1186 g         1153 t
ORIGIN      
        1 cccaaacaga agatattaaa accgaggaat ccgaagcagg agaggaggaa atggcatgac
       61 cgccgtatta aagcatgagc tccgcaatta tttccatacc ctgaccgcct atgtattcgg
      121 cgcatttttg ctggccttta tcggtctggg agccacctta tataatcttc aggcagcagt
      181 aagcaatttt gagtttgtat taagcttcgg cagcctggta tttgtagtaa ttgtacctat
      241 tttaaccatg cgcgtcattg cagaggagaa gaagcagaga acagaccagc tgttatactc
      301 ccttcccatt accaccacag aggttgtact gggaaaatat ctggcgcttc ttgttgtata
      361 tctgatccct ttggcggttg tttccgtata tccgctgata tttgcccgct atggagatgt
      421 ataccttttg acctcctacg gctctatctt tgcctttttt gttttgggcg cagccttgat
      481 cgctctggga gtattcattt cctccctgac agacaatcag ggctttgcgg caggaatcgg
      541 cattgcggtg atcctgctca actactacag cgccagtctg tccgagtatg tatcttctac
      601 tccggcaggc gccctgatcg cagcctttgc tctggtgatc gtcctgggag tggtaatacg
      661 ccatttaaca aaaaatgaac atctggctta tggattttat ttcctggctg gcggcgcagt
      721 gcttatttta tatctggcag acccggaagc tttttccgga cttctgccct cagttatgaa
      781 aacgctttcc ctgtttgaac gtttttatgt gtttgtaaac ggggtgtttg atctcactgc
      841 gatcgtatat tttgtgacct ttgccgcatt tttcctgttt ttatcggtgc agtcactgga
      901 aaaaaggagg tataactgat gaaacagaac cgtaatgcct tttgcggcgg agcctattcc
      961 ctgattcttt ccgcagtggt tcttacaatc cttattgtag tcaatatact ggtaaacgcc
     1021 cttccggaga atctgacaaa atacgatatc agcgcggcaa agctctattc cattaccagc
     1081 aatacaaagg cggtggtaaa cgggctggat gaggatgtaa ccatttactg gatcgtgcag
     1141 gcagacaaag aggatgcggt cattgaaaac ctgttaaata aatacgacag cctttcagac
     1201 catatccaga tcgtaaagaa aaatccggat gtatatccta cctttgcaga gcagtacaca
     1261 gatgaagagg ccgccaacaa cagcctggtt gtagaatgcg gcgaacgcag ccgttttatc
     1321 ggttatgatg atatttatgt tcaggaggca gatatctact cctattccta caatacctcc
     1381 tttgacggag agggagccat tacttctgcc attgactatg tggtaaatga agagcagccc
     1441 aaagtatatc ttttagaggg acatggggaa agagaacttc ccgcctcctt caaggagcag
     1501 attgaaaagg aaaatatcca gttagagcag ctgtctcttc tgaatgtaga ttccattcct
     1561 gaggatgcag ccggaattat gatctatgcg ccctccagcg acatttccgg agaagaacag
     1621 accatgcttg cagattacac caaaaaggga ggaaagcttt ttgcggcagc agggcctgta
     1681 aaggaaggaa atcttgacaa ccttcacggc cttttagagg aatacggcgt taccgcacag
     1741 gacggaatcg tagtagaggg agacagagag cgctatgctc ttcagacgcc ttacgccctt
     1801 cttcctcaga tggagagcag cgatattaca gacgctctca ttgaagagca ctattatccc
     1861 attcttccca ttgtccaggg attaaaggta gatgaaacga aagccgcagg ccgggtgaca
     1921 acccttctgt caaccacaga ccaggctttc agcaagatgg caggctatga gctttccacc
     1981 tatgaacggg aggaaggaga cgttgatggc ccctttgcgg cagctgtgtc gatttcctgt
     2041 gaaaatgaag gacggatcct ctggtttggc tcctcagact ttttagagga tatgtataat
     2101 gccctttcct ctggtgcaaa cggggatctg ggaatgaatg gtctttcttc catgatcgga
     2161 gagagcgagg ctatggcgat ccggagcaag tccttaaact ataattatct taccatcagc
     2221 gaatctgctt ctgccatgct tcaggcggta atgatcggtg ttttcccgct tgcatacctg
     2281 ggaattggca tctgtgtggt aatgagaagg aggagaatgc agaatgaagc gggctaaacg
     2341 gttatatatc cttctgggag ttctggcggt gctgtccgtg gcggccatcg cggtgatcca
     2401 gagggaggag cataaggaaa agatcaaaga cagcggtgaa accatccttc agattcccta
     2461 tgagggtgtc acggcccttt cgtgggaaaa tgagtcggaa agcctttcgt tccataaggg
     2521 agaggacgga gtctggacct atgacgagga tgaaaaattt cctgtgagcg aagaaaagat
     2581 aaaagaactc ttagagccct ttgaggaatt tgcagcagcc tttgtgatcg aagaggtaga
     2641 ggattacagt cagtacggtc tgaatgatcc tgtctgcacg attcatctgg aaaatgggga
     2701 gcaggagctt gaaataaagc tgggcaattt cagcaatatg gattcccagc gctatgtgtc
     2761 catcggagac ggaaaagctt atctggtaaa ggacgatccc ctgaattatt ttgacgcggt
     2821 gttaaaggat atgctgaagc aggatgagat ccctgagctt gagacagtaa gcagcctgcg
     2881 gtttacagga ccggagacct atgaagcagt ccgcatagag gacagcgggg cttcctacag
     2941 cagcgaggat gtttatttta agaaggatga ggaaaacctg cttcccttag atccggtaca
     3001 ggtggatgaa tatatcagaa ccctccagaa tctggatctg tctgactacg cttcctacta
     3061 tgtttcagag gaagagtgga gcacctatgg actggatgcg ccggagctgt ccttagaggc
     3121 cgattatacc tttgaaaatg aggacaaaga gaacgtatcc ggtacgctta ccgtaagcgt
     3181 aagccgtgat ccgaaggaaa aagaaaaggc agagaagaag gagaactttg aggaaaacag
     3241 cggggaagag gaagaaatta ccgcatacgc aagagtaaat aattcccaga tcgcctataa
     3301 gctgactgcc gaggattaca aaggcctgat ggccatgaaa tatgatgacc tgcgccataa
     3361 agaagtattc tggggcgata cagaggagat cacaggaatt gacatttcct tagagggagc
     3421 tgactacagc cttacttcca aaggcaaaaa ggatgaccgc acctggacgt atcaggagga
     3481 ggaaatagag gcagatgaac tgctctttgc tctggaaggc ttaaaagcag accgctttac
     3541 agaagaggag ccaggacaaa aggaggaaat ccgcctcact ctccatctgg ataatgaagt
     3601 ttctcctcag gtaaccatcg ttctttatcg ctatgacggc agcagctgtc tggcagaggc
     3661 tgatggaaag accgtttctc tgattcccag atctcaggca gtggatctca tcgaggcagt
     3721 aaataccatt gttttgggaa atacagagga ttagcccttg catttccctg cgtccgtgct
     3781 ataatgttca tataaaataa aaatataata gaaatgaaca aaaatgtcag tggacagggg
     3841 agcaaagatt cctctgtccg ctgacagtgc atatacagga tgaagaatgg aggatcatat
     3901 ggacagattg gggttgatat gcagaaacat tttggaggag ccatatatta ctcagcggga
     3961 gctggcaaag agaatggagc tatctttagg gaccattaac gggctgatca aagaatgtac
     4021 ggaaaaaggc tatatcagag aagatgagga tcagagagga aaacggcttg ctcttacaga
     4081 aacgggacag gctgttttag cgccctataa ggtagacgga gctctgatca ttgccgcagg
     4141 ctttggctcc cgttttgtcc ctcttacctt tgaaacgccc aaggggcttt tagaggtatt
     4201 tggagagcgt atgatcgaac gccagatccg ccagctccat gaggccggag tatatgatat
     4261 taccattgca gtcgggtatc tgaaagagaa atttgaatat ctgatcgaca aatatgatgt
     4321 aaagcttctc tacaatccgg agtattcctg caaaaatacc ctggctacgg tttacagagc
     4381 cagacatctg ttaaagggcc gcaatatgta cattttatcc tctgataact ggctgcggga
     4441 caatatgtac cacgcctatg agtgcggagc ctggtacagc gcctccttcc agaaggggga
     4501 taccaaggag tggtgcttta cctttactaa aaagggaagg attacagatg taaatgtagg
     4561 cggaagggat cagtgggtga tgta
//
LOCUS       DBMDUH010000182         4486 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_7162_length_4486_cov_6.740479, whole genome shotgun sequence.
ACCESSION   DBMDUH010000182 DBMDUH010000000
VERSION     DBMDUH010000182.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 4486)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4486)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4486
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7162_length_4486_cov_6.740479"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="ACZPCG_11975"
     CDS             complement(<1..209)
                     /locus_tag="ACZPCG_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007705268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HIR1285803.1"
                     /translation="MKVFENDADWEERAWGLAKKLVSIDSSDPGAYEGEIGERLYEYL
                     SRCCERCGGSLADQIQIAREEVLPGR"
     gene            complement(206..367)
                     /locus_tag="ACZPCG_11980"
     CDS             complement(206..367)
                     /locus_tag="ACZPCG_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285804.1"
                     /translation="MTNVRKLWTFADGMFTVPEGRFVVKGESASGLVLPEKEAYNSSI
                     GTRMGGSRI"
     gene            500..1363
                     /locus_tag="ACZPCG_11985"
     CDS             500..1363
                     /locus_tag="ACZPCG_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIR1285805.1"
                     /translation="MKEIKNPFWRTAAEYGTITISIWIMVIGIYFFKFPNHFAFGGIT
                     GFSTVISEMTQWSASDFTFITNMSLLVLGFIFLGRSFGLKTVYATVVMSVSLSLLERI
                     CPLTKPLTSEPLLELMFAIFLPAIGTAILFNIGASSGGTDIIAMIMKKHTSMNIGTVL
                     MLVDVASVLASFFIFDAETGLFSTIGLAAKSLVIDDVIENINLCKCFNIICDNPDPIC
                     DYIIHTLNRSATVYHAEGAFSHNDKTVIMTTMKRSQALKLRNYIRKIEPTAFMLISNS
                     SEIVGKGFLAS"
     gene            1485..2867
                     /gene="rpoN"
                     /locus_tag="ACZPCG_11990"
     CDS             1485..2867
                     /gene="rpoN"
                     /locus_tag="ACZPCG_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HIR1285806.1"
                     /translation="MDLKLQTRQTQTLSQRMIQSAEILQMTAQELNTYINETALENPV
                     MEISDSSSGGPDRESIEQQQWLNSFNEENYYLYQRQNNDDDYDFKDSWNIDTDDGETL
                     EDYLWSQLISEELSADDTQIIHYMLESLDDRGYLTESPGDIAADLKTDEAHILKLLAQ
                     LQKLDPAGVFARNLEECLNLQLERRGLLTPVMKTLTCQCLEMIAKNQIPAIARKLRLS
                     PAEAADCCQIIRSLNPKPGMSFSSREQLRYIIPDVTIVKFKDHFDILLNEAMYPAIEI
                     SSYYRKMSQEPESEEVKEYLGNKLRQAEWIRQCIAQRSLTLMDVSKAILEHQEAFFSK
                     GSNHLVPLRMTDIADRLGIHESTVSRAVSHKYLQCSWGVYPLSFFFSRSVSRSENGLN
                     SASHGINAAAIKLALRKIIAGEDPKKPYSDRLLSEKLASQGITISRRTVAKYREEEGI
                     LDASGRKEYI"
     gene            2965..3037
                     /locus_tag="ACZPCG_11995"
     tRNA            2965..3037
                     /locus_tag="ACZPCG_11995"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2998..3000,aa:Phe,seq:gaa)
     gene            3042..3112
                     /locus_tag="ACZPCG_12000"
     tRNA            3042..3112
                     /locus_tag="ACZPCG_12000"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3074..3076,aa:Gly,seq:tcc)
     gene            3136..3207
                     /locus_tag="ACZPCG_12005"
     tRNA            3136..3207
                     /locus_tag="ACZPCG_12005"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3168..3170,aa:Gly,seq:gcc)
     gene            complement(3257..4123)
                     /locus_tag="ACZPCG_12010"
     CDS             complement(3257..4123)
                     /locus_tag="ACZPCG_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007714973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR1285807.1"
                     /translation="MAVCGGICWGFSGCCGQFLFETRGAKAPWLVALRLLFAGLIMIV
                     MGFALHGKRNLDVLKKKADLLRLLIFAFCGISLCQFTYFMAIEASNAGTATVLQYLSP
                     ILILSAVCIKEKRRPVGLELGAIGLSLLGTFVLGTHGNIHTFYITGSALFWGLAAAVS
                     AVLYNMLPGSLILRYDIYQVLGFGMFFGGVLMCLIFRPWEYELIWDGGTWGALVSVIV
                     IGTAIAFGLYLQGVSMIGPLKGSILASVEPLSAVIISFFWLGTPFVMPDFLGFGMILG
                     AVLLLTAAQGRR"
     gene            complement(4199..>4486)
                     /locus_tag="ACZPCG_12015"
     CDS             complement(4199..>4486)
                     /locus_tag="ACZPCG_12015"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007705195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HIR1285808.1"
                     /translation="GQVFYYKNSQKGKTLAEILQKRFNYVLGEKNSRQAKANGNYYLL
                     LHVKPPIAIVECGFLSNRAEAAKLQDEVYQDRMAWTVHMGIMEFLNRDFAG"
BASE COUNT         1207 a         1170 c          934 g         1175 t
ORIGIN      
        1 cggcccggaa gtacctcctc ccgtgcgatt tggatctggt ctgccagaga accgccacac
       61 cgttcacagc accggcttaa atactcataa agccgttccc caatctcgcc ttcataagcg
      121 cccggatccg agctgtctat ggacaccagc ttttttgcca gcccccaggc ccgctcctcc
      181 cagtccgcat cattttcaaa taccttcata tcctgcttcc tcccattctt gttccaatac
      241 tgctattata ggcttccttt tccggtaata caagaccgct ggcagattct ccctttacta
      301 caaaccgtcc ttccggaaca gtgaacatac cgtctgcaaa tgtccacagc ttgcggacat
      361 ttgtcacaac cttttcgttg tacctatttt agtaaaaaag gtggttgcgc ttgtagtaaa
      421 acacgcttat tatatggttg aatcaaaatg catattttga atgaacaaga gtattaaaaa
      481 ggaggtttat tctttcatca tgaaagaaat caaaaatcca ttctggcgca cagccgctga
      541 atatggaact atcacaatca gtatctggat catggttata ggaatctatt ttttcaaatt
      601 cccaaaccac tttgcctttg gcgggatcac cggcttttcc accgttatca gtgagatgac
      661 ccaatggtcg gccagtgatt ttacattcat tacaaatatg tcccttctgg ttcttggctt
      721 tatctttctt ggaagaagct ttggactgaa aaccgtttat gccaccgttg ttatgtccgt
      781 cagtctttcc ctcctggagc gtatctgccc attgacaaag cctctgacct cagagccgct
      841 tctggaactg atgtttgcaa ttttcctgcc tgccatcgga accgctattt tattcaatat
      901 cggagcatcc agcggcggta cggatatcat tgccatgatc atgaaaaagc acaccagcat
      961 gaatataggc accgtactga tgctggtaga tgtggcatcc gttctggctt cctttttcat
     1021 ctttgatgcg gaaacaggac tgttctccac catcggcctt gcagctaaat cccttgtgat
     1081 cgatgatgtg atcgaaaata tcaatctctg caaatgtttt aatatcatct gcgacaatcc
     1141 cgatcccatc tgtgattaca tcatccatac cttaaaccgc agcgccacag tctaccacgc
     1201 ggagggagca ttttcccaca atgacaagac cgtgatcatg actaccatga aacgcagtca
     1261 ggccttaaag ctgcgcaact atataagaaa aattgaaccc actgccttta tgctcatctc
     1321 caattccagc gagatcgtag gaaaaggctt tctggcaagc tgatctggaa tttccagcgc
     1381 ctcctttatc tttatgttaa gcggggcgct ctttttgcat tcaaaaacag aacgatctgt
     1441 gatatactta aaccaatcat atcttacgaa agaaggtgct ttccatggat ctgaagcttc
     1501 agacccgaca gactcagacc ctatcccaac gcatgataca atctgctgag atcctgcaga
     1561 tgactgccca ggaattaaac acatatatca atgagacggc actggaaaat cccgtgatgg
     1621 aaatatctga ttcttcttcc ggagggccgg atcgggaatc cattgaacag cagcagtggc
     1681 ttaattcctt taatgaagaa aactattacc tctaccagcg gcagaacaac gatgatgact
     1741 acgattttaa ggattcctgg aacattgata cggatgacgg cgagacgctg gaagactatc
     1801 tctggtccca gctgatctca gaggagcttt ctgccgatga tacacagatt attcactaca
     1861 tgctggaatc gttagatgac agaggctatc tcaccgaatc tcccggagat attgcggcag
     1921 atcttaaaac agacgaagca catatcctta agcttcttgc gcagctccag aaactggacc
     1981 ctgcaggagt ttttgcccga aatctggaag agtgtttaaa tcttcagctg gaacgccgcg
     2041 gcctccttac acctgtgatg aaaacattaa cctgtcagtg cctggaaatg attgccaaaa
     2101 atcagatacc agccattgcc agaaagcttc gtctgtcccc tgcggaagcc gcagactgct
     2161 gccagatcat ccgctccctg aatcccaaac ccggaatgtc cttttccagc cgggaacagc
     2221 tccgctatat tattccggat gtaaccattg tcaaatttaa agaccatttt gatatcctct
     2281 taaatgaagc catgtatccg gccattgaaa tcagcagcta ttaccgcaaa atgagccagg
     2341 aacctgagtc tgaggaggta aaagaatatc tgggaaataa gcttcgtcag gcagaatgga
     2401 tccgccagtg catcgcacag agaagcctca ccttgatgga tgtgtccaag gctattctgg
     2461 agcatcagga agcatttttc tcaaagggtt ccaatcacct tgtcccgctg cgcatgactg
     2521 atatcgcaga ccggctgggg atacatgagt ctacggtcag cagagccgtc agccataaat
     2581 atctccagtg ctcctggggt gtatatcccc tgagcttttt cttttcgcgc tccgtttccc
     2641 gcagtgaaaa tggtttaaac tccgcctctc atggcatcaa tgccgcagcc atcaaactgg
     2701 ctctcaggaa aatcatagcc ggagaagacc ccaaaaagcc ctacagcgac cgccttctaa
     2761 gcgaaaaact ggcttcccag ggtatcacca tctccaggag aaccgttgca aaatacaggg
     2821 aagaggaagg aattttagac gccagcgggc gaaaagagta catttaacaa ttttttttaa
     2881 atattttacc aaaagacttg acagcttaca aaaaaaaggg tagaatatca ccgttgtcag
     2941 cgattttcaa tcactgatac ggatgcccag atagctcagt tggtagagca gaggactgaa
     3001 aatcctcgtg tcgttggttc gattccgact ttgggcactg cgcaggtgta gttcaatggt
     3061 agaacactag ccttccaagc tagatacgtg ggttcgattc ccatcacctg cttgaaaaca
     3121 gaatatctgt tttatgcgcg agtggctcag tggtggagca ccaccttgcc aaggtggggg
     3181 ccgcgggttc gaatcccgtc tcgcgctttt tttattttca gcggaagcct ttattttcaa
     3241 aggtttccgc tgttttttat cttctcccct gcgccgccgt caaaagcaaa actgctccta
     3301 agatcattcc aaatcccagg aaatccggca ttacaaaagg agttcccagc cagaaaaatg
     3361 agatgattac cgcagataaa ggttctacgc ttgccaggat gctccccttt aaggggccga
     3421 tcatggacac gccctgaaga tacagtccaa aggcaatggc tgttcctatt acaatcacgc
     3481 tgactaatgc tccccaggta cctccgtccc atatgagctc atattcccac gggcggaaaa
     3541 tcagacacat gagaacacct ccgaagaaca ttccgaatcc caacacctga tatatgtcat
     3601 aacgcaggat caggcttccg ggaagcatat tgtaaagtac cgcgctgaca gcggctgcaa
     3661 gcccccagaa aagagcgctt cccgtaatat agaaggtatg tatattgcca tgagtcccaa
     3721 ggacaaaggt tcccagaagg gaaagaccta tggctcccag ttccagtcct accggacggc
     3781 gtttttcctt tatgcagact gcagacagta tcagaatggg ggacagatac tgcagtaccg
     3841 tagccgttcc cgcattcgat gcctcaatgg ccataaaata cgtaaactgg cacaggctga
     3901 tgccgcagaa ggcaaagatc agcaggcgaa gcagatccgc ttttttcttt aatacatcca
     3961 gatttcgttt tccatgaagg gcaaatccca tcacgatcat aataagccct gcaaaaagca
     4021 ggcgcagcgc caccagccat ggcgctttgg ctccccttgt ctcaaataaa aactgtccgc
     4081 agcagccgga aaaaccccag cagattcctc cgcagactgc cattacagtc cccagtgttt
     4141 cttttttctt ctgttccatt tcaaattttc tcccttgtta tcttatcctt ttgtctgttt
     4201 atcctgcaaa atctctattt aaaaactcca taattcccat atgcaccgtc cacgccattc
     4261 ggtcctgata cacttcatcc tgcagtttgg ccgcctcagc ccggttgctg aggaaaccgc
     4321 actctacaat ggctatgggc ggttttacat ggagaaggag gtaataattt ccatttgcct
     4381 tggcctgccg actgttcttc tctcccaaaa cataattaaa acgcttttgg agaatttccg
     4441 ccagcgtttt tcctttttgt gaatttttgt aataaaatac ctgacc
//
LOCUS       DBMDUH010000183         4454 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_7217_length_4454_cov_4.880083, whole genome shotgun sequence.
ACCESSION   DBMDUH010000183 DBMDUH010000000
VERSION     DBMDUH010000183.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4454)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7217_length_4454_cov_4.880083"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(158..376)
                     /locus_tag="ACZPCG_12020"
     CDS             complement(158..376)
                     /locus_tag="ACZPCG_12020"
                     /inference="COORDINATES: protein motif:HMM:NF042639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6578 domain-containing protein"
                     /protein_id="HIR1285809.1"
                     /translation="MKCTVFYESWQLECCGKAFSIGDIVKWFVYKIEREQILAPVDLG
                     TIDYCYEAHDENWEKLFVRKALKRKSKI"
     gene            complement(467..1063)
                     /locus_tag="ACZPCG_12025"
     CDS             complement(467..1063)
                     /locus_tag="ACZPCG_12025"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HIR1285810.1"
                     /translation="MNEWNKAQAGYLYDANYDREVVENRTKCADLCYEFNQCKPSDTK
                     KQAEIIKKIIGCIKGDFVITAPFYCDYGSNITIGNNFYTNHNCTILDGAKVTFGDNVF
                     IAPNVVFSTAGHAIDAGQRAKGLEIALPITVGDNVWIGANVSVLPGVSIGSNTIIGAG
                     SVVNKDIPDGVIAAGVPCRVIRRITEKDRQKYPEYIEI"
     gene            complement(1117..1908)
                     /locus_tag="ACZPCG_12030"
     CDS             complement(1117..1908)
                     /locus_tag="ACZPCG_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285811.1"
                     /translation="MNVMKIVSPKTFIVSLLLGILVLYKVLSIDGFTDILWISLMGYW
                     TIRGMRVAFSQESAVLIFAAGYAVWIALYISKHKKKAVETGSWETGKLNADNERAWKL
                     SDLCHNVIYFIAAVFLIFYFIFGDPMIYINNYRLRTAMTKIQNDRVTLEETIPFQWTR
                     VYSFGPYFPLKDIERITGSKSPALDDSISEGMVSLIFMDHGRVVSSVCRYPSSTGYSL
                     NLAVNAASDFDQYDNYSRMDYGDLTEFEVTLEDGIVNLTADKPDI"
     gene            2128..3003
                     /locus_tag="ACZPCG_12035"
     CDS             2128..3003
                     /locus_tag="ACZPCG_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR1285812.1"
                     /translation="MKKRVVCAALVSIMTMLLAACSGGAVSAPAETKAESTEAKTEAA
                     ETTEKAAEAEGETKAEGGTLIVGFDQDFPPMGFMGDDGEYTGFDLELAQEAAKRLGLE
                     YKPQPIAWDAKDMELESGNIDCIWNGFTMTGREDGYTWSEPYMENSQVFVVAGDSGIK
                     SQADLAGKVVECQVDSSAEAALKEVPDLTATFAQLLTTADYNTAFMDLEQGAVDAIAM
                     DVVVAGYQISQRNADFVILEDSLAAEEYAIGFKKGNTELKDKVQGALEEMAADGTLKT
                     ISEKWFGKDITTIGK"
     gene            3020..3679
                     /locus_tag="ACZPCG_12040"
     CDS             3020..3679
                     /locus_tag="ACZPCG_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR1285813.1"
                     /translation="MGKMTLSQMVAQLAGGMGTSIQIFVVTLVFSLPLGLIVAFGRMT
                     KNGFLRNLVKVYISIMRGTPLMLQLMVVYFGPYYLFGIRVGSGYRLWATFIGFVINYA
                     AYFAEIYRSGIQSMPQGQYEAAYILGYSRFQTFFKIILPQVIKRILPSITNEVITLVK
                     DTSLAFTLSVVEMFSVAKALASSQTSMIPFVAAGLFYYIFNLIVACTMEWMEKKLEYY
                     R"
     gene            3699..>4454
                     /locus_tag="ACZPCG_12045"
     CDS             3699..>4454
                     /locus_tag="ACZPCG_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIR1285814.1"
                     /translation="MKLLELNHVEKSFGDLKVLKDISMSVEDGEIVSIIGPSGSGKST
                     LLRCATMLETMDRGEVVYLGKKAVWGNDSGSIQYASPAELKEIRSQYGLVFQNFNLFP
                     HFSVMKNITDAPIHVQKRNKEEVYSEARELLKKMGLEDKEDAYPYQLSGGQCQRVAIA
                     RALALKPRILFFDEPTSALDPELTGEVLKVIRSLADLHIAMVIVTHEMAFARDISHRV
                     VFMAGGVIVEEGTPEEVFSSDNERTQSFLGRYGT"
BASE COUNT         1253 a          990 c          981 g         1230 t
ORIGIN      
        1 tcataccaca taataacatc aagaaacgct aagatattac agactatact gcacccaaaa
       61 ggcagcttca aaaacggaag ctatttgaat gtaacttctt cctgtttgga atgtctgatt
      121 gaacattcat ttaactcaac aatataccct gttgctctca tatcttcgat tttcttttca
      181 aagcctttcg aacaaataat ttttcccaat tttcatcatg agcttcataa caatagtcaa
      241 tcgttccgag gtctacaggg gcaagtatct gttcacgttc tattttataa acgaaccact
      301 tcactatatc acctatagaa aaagctttac cacagcattc cagctgccaa ctctcataaa
      361 atactgtaca tttcattttc actgtcacct tacaactttc gactttcctg tttctatttc
      421 agctttcagc ccagttaatt cccaattccg taaaccagaa aattcgctat atttctatat
      481 actctggata tttttgcctg tctttctccg ttattctgcg aattaccctg cagggaacgc
      541 ctgcggcaat tactccatca ggtatatctt tattcacaac gcttccagca cctattattg
      601 tattgcttcc gatcgaaact cccggcagca cagatacatt tgcaccaatc catacattgt
      661 caccgacagt aattggaagt gcgatttcca gcccctttgc tctttgcccg gcatctatgg
      721 catgaccagc cgttgaaaaa acaacattag gagcaataaa tacattatcg ccaaaggtaa
      781 ccttagcccc atccaatatt gtgcagttgt ggtttgtgta gaaattattc ccaatcgtta
      841 tattgctccc atagtcgcag taaaagggcg ctgtaattac aaagtctcct ttaatacatc
      901 caattatttt ctttataatt tccgcctgtt ttttagtatc cgatggtttg cactgattaa
      961 attcatagca caaatcagcg catttcgttc tattttccac aacttccctg tcatagttgg
     1021 cgtcatataa atagcctgcc tgagctttat tccattcgtt catttcattc cctcatcctt
     1081 aagtttatac catggttcat acgcagcgga tcccctctaa atatccggtt tatcagctgt
     1141 cagattaaca attccatctt ccagcgttac ttcaaattcg gttaaatctc cataatccat
     1201 ccttgaatag ttatcatatt gatcaaaatc agaagcagca ttaacagcaa gatttaatga
     1261 ataccctgta gatgaaggat atctgcatac actggaaacg actctgccgt gatccataaa
     1321 tatcaggctt accattcctt cactaatgct gtcatcaagc gcagggcttt ttgatccggt
     1381 aattcgttcg atatccttta aagggaaata gggtccaaag gagtatactc tggtccattg
     1441 aaatggtatg gtttcttcca gtgtaacccg gtcattctgt atctttgtca ttgctgtcct
     1501 cagcctgtaa ttatttatat aaatcatcgg atccccgaaa ataaaataaa agatcagaaa
     1561 cacagcggca ataaaatata taacattatg acaaagatct gagagcttcc atgcccgttc
     1621 attatcggca tttaattttc cagtttccca acttccggtt tcaaccgctt ttttcttatg
     1681 ctttgaaata tacagagcaa tccacacggc atagcccgct gcaaagatta aaacagccga
     1741 ctcctgtgaa aatgcaaccc gcattcccct tatggtccaa tatcccatca gactaatcca
     1801 cagtatgtcg gtaaatccat caatggacaa cactttatag agaacaagta ttccaagcaa
     1861 taatgaaacg ataaatgttt taggacttac tattttcatt acgttcactc cattcttcta
     1921 aaaaccatga taccacactc ccaccaccat ggaaaggctg cgggaaaata acttcggaag
     1981 tacggaagcg gaaataattt tcattttccc ctttacaaaa atactttagt atgctaatat
     2041 gataacacat tagcaaatga gagccgacac gatttatgga gattaaaagc aggaagccaa
     2101 aggctcgcca tcgaggagga aacgattatg aaaaaaagag ttgtatgtgc cgcacttgta
     2161 tctataatga ccatgttact ggcagcctgc tccggtggag ccgtatctgc accggctgag
     2221 acaaaggctg aatccacaga agcaaaaaca gaggcagcag agaccacaga aaaagccgct
     2281 gaagcagaag gagaaacaaa ggcagaaggc ggtaccctga tcgtgggatt tgatcaggat
     2341 ttcccgccga tgggatttat gggagatgat ggcgagtata caggctttga cctggagctg
     2401 gcacaggagg ctgcaaagcg cctgggtctg gagtataagc cccagcctat cgcatgggat
     2461 gcaaaggata tggaactgga gtcgggaaat attgactgta tctggaatgg ctttaccatg
     2521 accggccggg aggacggata tacatggagc gagccctaca tggaaaattc tcaggtgttt
     2581 gtagtggcag gggattccgg aattaaaagc caggcagacc ttgcaggaaa ggtagtggag
     2641 tgtcaggtgg attcctccgc ggaagcagcc ttaaaggagg ttcctgatct gacagccacc
     2701 tttgcacagc ttcttactac cgcagattac aacacagcct ttatggatct ggagcagggc
     2761 gcagtagacg ccattgccat ggatgtagtt gtggccggat atcagatcag ccagagaaac
     2821 gcagattttg ttatactgga ggattcgctg gcggcagagg agtatgcgat cggctttaag
     2881 aagggaaaca cagagttaaa ggataaagtc cagggcgcat tggaggagat ggctgcggac
     2941 ggcaccttaa agaccatctc tgaaaaatgg tttggcaagg acattacgac aattggaaaa
     3001 tgaaagcagg tagtttaaaa tgggtaaaat gacactttcc cagatggttg cacagttagc
     3061 aggaggcatg ggaaccagca tacagatatt tgtggttacg cttgtatttt ctctgccttt
     3121 gggtctcata gtggcttttg gccgtatgac gaaaaacggt ttcctcagaa atctggttaa
     3181 ggtttacatt tctattatga ggggaacgcc cctgatgctg cagctcatgg tggtttattt
     3241 tggcccttac tatcttttcg gcatccgtgt aggaagcggc taccgtctct gggctacctt
     3301 tatcgggttt gtgattaact atgccgccta ttttgcggaa atttaccgca gcggcattca
     3361 gtctatgcct cagggacagt atgaggcggc ctatatcctg ggctacagcc ggttccagac
     3421 cttttttaag atcattctgc ctcaggtgat caagcggatc ctcccctcga ttaccaatga
     3481 ggtgattacc ttggtaaaag acacgtcgct ggcgtttacc ctaagcgtag tggagatgtt
     3541 ttcagttgca aaggcgctgg catcctctca gaccagtatg attccctttg tggcagccgg
     3601 ccttttctat tacatcttca atctgattgt ggcctgcacc atggaatgga tggaaaaaaa
     3661 gctggagtat taccgttaga aacaggagga tgtgggaaat gaagcttttg gaattgaatc
     3721 atgtggaaaa gtccttcggc gatctgaagg tattaaagga tatatcaatg agcgtggagg
     3781 acggggagat cgtttccatc atcggccctt caggctccgg caaatcaacc ctcctgcgct
     3841 gtgccaccat gctggaaacc atggatcggg gcgaggtggt ttatctggga aaaaaggcgg
     3901 tatggggaaa tgattccggt tccattcagt atgcatctcc tgcagaatta aaggaaatcc
     3961 gcagccagta tggtctggta tttcagaatt ttaatctgtt tcctcatttt tcggtgatga
     4021 aaaatatcac agatgcgccg atccatgtac agaagcgcaa taaggaggag gtctattcag
     4081 aagccagaga gctgttaaaa aagatgggac ttgaagataa agaagacgcc tatccatacc
     4141 agctttccgg cggtcagtgc cagagggttg ctatcgcaag agccctggcc ttaaagccca
     4201 ggatcctgtt ttttgatgag cctacctcag ctctcgatcc ggagcttacg ggagaggtgt
     4261 tgaaggtgat ccgctccctg gcagatctcc atattgccat ggtgatcgta acacatgaga
     4321 tggcctttgc cagagatatt tcccacaggg tcgtctttat ggccggaggg gtgatcgtgg
     4381 aagagggaac accagaggag gtattttcct cagataatga gcggacgcag agctttctgg
     4441 ggagatatgg aacg
//
LOCUS       DBMDUH010000184         4300 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_7501_length_4300_cov_5.040962, whole genome shotgun sequence.
ACCESSION   DBMDUH010000184 DBMDUH010000000
VERSION     DBMDUH010000184.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 4300)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4300)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4300
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7501_length_4300_cov_5.040962"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            37..1170
                     /locus_tag="ACZPCG_12050"
     CDS             37..1170
                     /locus_tag="ACZPCG_12050"
                     /inference="COORDINATES: protein motif:HMM:NF021028.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HIR1285815.1"
                     /translation="MEYSLNGGAWKKGYSPPVKYERGETVILPEKSELVYGGYSFSGW
                     FRSPDFSGEPAVQIGPDESGDILLYARWECDHSQGTDMKYDGQTHWFYCRVCGKITEY
                     GNHSFSSLLIKEPDCITNGIHRYSCRCGYEYDAPDVAALGHAWKNGLDYNETYHVRFC
                     SRCRAEREKADHSLLWQENGSQCWQGCSGCGYTRNRYNISLMYNIGAMAASDWWSQSN
                     QIKVESRSVSIRPGTMLLVSADYRVDSSPFRFNVDFYPDEAGCEIHLSGETYRQHINW
                     WTGAMPGYATQFRLFDNLNTSCNPSNRAILENCRIYKVNTAAGVWMPAKKGLYRIPEG
                     MTATPSQAGKKTPSQAGRKTPSQAAKKTPSQIKRKSPSQLCRF"
     gene            complement(1174..1821)
                     /locus_tag="ACZPCG_12055"
     CDS             complement(1174..1821)
                     /locus_tag="ACZPCG_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004842258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR1285816.1"
                     /translation="MNLSNTQLFRGLEEHEITSLLSCLNTVTRSYKKGEIILSEGMIT
                     ENIGVVLSGMVLISYGDIWGNTSILGNAAPGCVFAEVYACIPGQPLLITVSAAEPATV
                     LFMNVGRVLTSCSNTCPFHARLARNLLTVCADKSLQLSRRMKHTTSKSIRGRLMSYFS
                     ECAKQFGSSSFIIPYNRQQLADYLGVDRSTMCNELSKMQRDGIIEYEKNRFLLKM"
     gene            2785..4152
                     /locus_tag="ACZPCG_12060"
     CDS             2785..4152
                     /locus_tag="ACZPCG_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009250339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1285817.1"
                     /translation="MVRSPSLVDMTSGSIIKKALIFAIPICVGNILQQLYSTVDTLVI
                     GNYCDAAALAAVATSSQPLEILLCIFLGIGSGISILVSQAMGRNDGKRMGRLTSTAVW
                     FLYLCAVPLSVVGVLAGPWLLKLMKVPADAMEGAVEYLRITTLGCLGNMGYNLNAGIL
                     RGLGNSSATLLLLMITCLVNIVLDLVFVAVFGMGVAGAALATAIAMIMSWLFSILYIR
                     KHYKELAMTFLPHSYDRAMVRQILSVGLPLGLNSALYSVGHLVMQTLFNTQGSAFVAG
                     CSVAGKVNAIANIAITSFSSAATIFAGQNLGAEDYRRLRRGALIIPVFSGAITLAAGL
                     LLTAGCRPVLRFFTRDEQVLEMAVHYIRVVLPFTWCYAVFNGIICFVNGIGEIRYPTI
                     VNLLMLWAVRIPAACLIGWLGYGRNVMAGIPISFVVGLLAMLLYFRTDKWKELCRKAE
                     KQEKK"
BASE COUNT         1143 a          839 c         1097 g         1221 t
ORIGIN      
        1 agggggcgca caaagtgaag tggcaaggga tattctattg aatacagtct taatggagga
       61 gcatggaaaa aaggatatag tcctccggtg aaatacgaaa gaggagagac tgtgattctg
      121 cctgaaaagt cagaactggt atatggagga tatagctttt caggatggtt tcgatcccct
      181 gatttcagtg gagagccggc tgtacagatc gggccggatg aatcaggtga tattttactg
      241 tatgccagat gggagtgtga tcattcccag ggaaccgata tgaaatatga tggtcagaca
      301 cactggtttt actgcagagt atgcgggaaa attacagaat acggaaatca tagcttttcc
      361 tctttgctga ttaaggagcc cgactgcatc acgaatggaa tccacagata ttcctgccga
      421 tgtggatatg agtatgatgc gccggatgtg gcagctctgg gtcatgcatg gaaaaatggt
      481 ctggattata atgagacgta tcatgtccgt ttttgcagca gatgtcgggc tgagagagaa
      541 aaggcagacc acagcctttt gtggcaggaa aatggttctc agtgctggca gggatgcagc
      601 ggctgcggat atacccggaa cagatataac atttctctta tgtacaatat tggagcaatg
      661 gctgcatcgg actggtggag ccagagcaat cagattaaag tggaaagcag gagcgtatcc
      721 atccggccgg gtacgatgct gttggtttca gcagattaca gggtggacag cagtccgttc
      781 cggtttaatg ttgatttcta tcccgatgaa gccggatgtg aaattcattt gtcgggagaa
      841 acttacagac agcacataaa ctggtggacc ggagctatgc ccggatatgc aacgcagttc
      901 agattatttg ataacttgaa tacctcctgt aatccttcaa acagggctat tttggaaaac
      961 tgcaggattt ataaagtgaa tacggcagca ggagtatgga tgccggcaaa gaagggactg
     1021 tatcgtatcc cggaagggat gacagcaact ccgtctcagg caggaaagaa aacgccgtct
     1081 caggccggta gaaaaacacc gtctcaggca gctaaaaaga ctccttctca aatcaaaaga
     1141 aaatccccgt cacagctttg ccgcttctaa cattcacatt ttcagcaaaa atcgattctt
     1201 ctcatattca atgatcccat ctctctgcat tttagaaagc tcattgcaca tggtactgcg
     1261 gtccacaccc agatagtctg ccagctgctg gcggttatag ggaattatga aggaactgct
     1321 tccaaactgt ttggcacatt ctgaaaaata ggacatcaga cgcccgcgga tggattttga
     1381 ggtagtgtgc ttcattcttc gggaaagctg aaggcttttg tcagcgcata cagtcagcag
     1441 gtttctggcc agccttgcat gaaaggggca ggtattggag caggaggtca agacccgccc
     1501 cacattcata aacagcacgg ttgcgggctc ggcggcagat acagtgatca gcagaggttg
     1561 tcccggaata caggcatata cttcggcaaa tacgcagccg ggtgctgcat tgcccagaat
     1621 acttgtattt ccccagatat caccgtagga gataaggacc ataccggaga gtacaacacc
     1681 gatattttcc gtgatcattc cctcggagag aatgatttct ccctttttat aactgcgtgt
     1741 cacagtattc agacagctta aaagagatgt gatttcgtgt tcctccagtc cacggaacag
     1801 ctgtgtattg gataagttca ttttttctcc ttttgttgta ataacaacag attttcatgt
     1861 ttgattatat cataataagt accgaagaag atgatttaaa gttgtttttt gattaaatgc
     1921 gtaatggata ctggaacttt aggataagag ccgtcttttg cggctctttt gtgtttgtgt
     1981 gtcacgggcg tgccttaact gtaagcaaat gttgtgaaaa atttgacagt tataatacat
     2041 actccatctg acagtcatat ggctcctctt cgacatgctt ccggctgtat atccttgtat
     2101 ccacacatcc aagagcgtcc ttccaattcc cattctccgc atatcctccg atacaaatct
     2161 ttttaaagta ttctgataaa aagcaccgca gacaaagcca ttggagcaga gcagggattt
     2221 taaatgggag ataatttgcc gatagtcatc ttctgaccag tcatctgtaa agggatcatc
     2281 ccggatgatc cagcttccat gctctctgcg ccagcatttt acaacggtct gatggcggat
     2341 gaagtgatca aaaaagcttc ggttcatgtc cttggtgtgc aaaattttat aagatatcat
     2401 agttttgttc cttgctgaat gtttgttgcc aagcttgctg catgtattct catgataccg
     2461 catccaggtt tgtatttcaa gtttcagaaa ctccgcccgt tttccagatt gtggacagta
     2521 gtttttatga cgcctgttgt ttttggaaaa aaggaaggta gctgcgggga taattgcatt
     2581 gttttttggg gagaagtacc gaataccctt ccgatttttg gaggtacaat taccataggg
     2641 ggtgtgctgc tgtattcctg tgtggagaag aggcgatgaa aaataagatt gaagttgtaa
     2701 tataacagtg ccggatattt cttgcatacg agaatagttt gtattagaat agactacatc
     2761 agttataaaa cggaggaagt atttatggtg cgttccccat cgctggtgga tatgaccagc
     2821 ggctctatta taaaaaaggc tcttattttt gccattccta tttgtgtggg aaatatcttg
     2881 cagcagcttt acagtacagt agatacgctg gttattggga attactgtga tgcagcagct
     2941 ttggcggcgg tggctacaag ttctcagccg ttggaaattc tgctttgcat ctttctggga
     3001 atcggatcgg ggatttcgat tctggtttcc caggccatgg gaagaaatga cggaaagcgg
     3061 atggggaggc ttacctccac ggcagtatgg tttttgtatc tctgtgcggt tcctctttct
     3121 gtggtggggg tgctggcagg tccctggctt ttaaagctga tgaaggtgcc ggcagacgcc
     3181 atggagggag cggttgagta tctcaggatc actacgttgg gctgtctggg aaatatggga
     3241 tacaatttaa acgccgggat attaagaggg cttgggaaca gcagtgcaac cctgcttctt
     3301 ttgatgatta cctgcctggt aaatattgtg ctggatctgg tttttgtggc tgtattcgga
     3361 atgggagtgg caggggcagc tctggcgaca gccatcgcaa tgataatgtc ctggctgttc
     3421 agtatactgt atatcaggaa gcattacaaa gagctggcta tgacgtttct gccccatagc
     3481 tatgacaggg caatggtcag acagattctg agcgtggggc ttcctctcgg attaaactca
     3541 gctttatatt cggtcggcca tctggtgatg cagacgttgt ttaataccca gggttctgct
     3601 tttgtggcag gttgttctgt tgcgggaaag gtgaatgcta ttgctaatat tgcgataact
     3661 tccttttcct ctgcggcaac catttttgca ggtcagaatc ttggggcaga ggattacaga
     3721 aggcttcgca gaggagcttt aataattcca gtattttcag gagctattac cctggctgcc
     3781 ggtcttttgc ttacagccgg ctgccgtccg gtgctccgtt tttttacaag ggatgaacag
     3841 gttcttgaga tggcagttca ctatattcgc gtggttctgc catttacctg gtgctatgcg
     3901 gtatttaatg ggattatctg ctttgtgaat ggaattggag agatccgtta tcctaccatt
     3961 gtgaatctgc tgatgctgtg ggcagtccgg attcccgcag cctgtctgat cggatggctg
     4021 ggatatggaa gaaacgtaat ggcaggaatc cccataagct ttgtagtcgg tcttctggcc
     4081 atgcttctgt atttccgaac agataaatgg aaggaattgt gcaggaaggc agagaagcag
     4141 gaaaaaaaat gaaagaagta acaaaatacg ctctgacaga agagaaaatg tctgtcgggg
     4201 cgtattttat ataaatatgt gaaaaatatt actatataag taacaaataa taaaatataa
     4261 tacgatattc acaataatca gaaactattt acatatcatt
//
LOCUS       DBMDUH010000185         4132 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_7820_length_4132_cov_5.863806, whole genome shotgun sequence.
ACCESSION   DBMDUH010000185 DBMDUH010000000
VERSION     DBMDUH010000185.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4132)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7820_length_4132_cov_5.863806"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="ACZPCG_12065"
     CDS             complement(<1..394)
                     /locus_tag="ACZPCG_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015513688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HIR1285818.1"
                     /translation="MKHIFLNLKRFDVPKEYGGVNAIAAVKDWGSYIVEQTQKGLMEY
                     GTEDVEFAMFFPEAHILTAAGTLCEGSPVKVGCQGVFRQDTAVGGNFGAFTTSRTANA
                     ARALGCSYVLIGHCEERKDKAGILAAGGG"
     gene            complement(391..1584)
                     /locus_tag="ACZPCG_12070"
     CDS             complement(391..1584)
                     /locus_tag="ACZPCG_12070"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006781534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HIR1285819.1"
                     /translation="MRDNVLVVHGGGPTAVLNASLYGVLLEARESGAVGAVYGAIGGS
                     EGILKERFLNLSRFPEEELKKLLTTPGTAIGSSRYALEEEDYKAMEAIFRKHHIRYVL
                     FNGGNGTMDACGKLVRALGPDSDIRVAGIPKTIDNDIGITDHTPGYGSTARYIARTTA
                     EIGADVKSLPIHVCILEAMGRNAGWITAASALARKKHGDAPHLIYLPERPFREEEFLA
                     DVKELYDRLGGVVVVASEGLKDESGKPVVPPIFQTGRSVYYGDVGAYLAELVIKNLGI
                     KARSEKPGLCGRASISLQSPVDREEAVLAGREALKAAMEGCTGIMIGFARREQEKYEV
                     CPIRIPVEQVMLTERTVPASYINNRGNDVTDAFVSWCRPLTGEEIPDYIDFQEIYRER
                     REDRE"
     gene            complement(1605..2426)
                     /locus_tag="ACZPCG_12075"
     CDS             complement(1605..2426)
                     /locus_tag="ACZPCG_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase family"
                     /protein_id="HIR1285820.1"
                     /translation="MKHDYYSYTKEQLLENPKMRLFCLEDNAAVFKQMAEQMAEEIKA
                     HNAEGKHTVFICPVGPVGQYPYFVDLVNEQNISLKNVWFINMDEYLDDEKRWVPEDHP
                     LSFRGFMNRTVYDRIRPELVMPKEQRVFPDPFDTERVPKLIEELGGVDIAFGGIGING
                     HVAFNEADASLTNEGFLAQKTRVLAITPETRTANAIGDFNGALEDMPHYCVTIGIYEI
                     AHARKIRLGCFRNWHRAVVRRAAYGEPSSDFPVSLLQSHPDLTLTFTDYVAALSD"
     gene            complement(2530..3618)
                     /locus_tag="ACZPCG_12080"
     CDS             complement(2530..3618)
                     /locus_tag="ACZPCG_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015513692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1285821.1"
                     /translation="MKKRFLAAAMAAVMALSVTACSGGSSAPETKAPESAEASKAEET
                     SKAETEKETKAEEEKTEAEPITLNIAYMPNYGSLWSVAAAQAQGFMEEEGIKLNLVSF
                     ADGPTIIAAMESGSIDIGYIGQGAHKLCVNGQAKIFALSHISNGDAVIGGSSVKTMED
                     LKGKKVAYSSGTSSEDILNNTLTKAGMTMSDIQAVDMDASGIVTAMISGGVDACATWS
                     PNSLKIMEEIPEATKLADNMTFKDTTVSLASWIVTPKYAEENQDTILRFTKALFKGMD
                     YAADDHYEETAKAVADITKTDEASVFDQRGDADWLTGKEVSEGAADGTVENYYKIQQE
                     NFIAGGQVEKEVPVADYVMLDNMIEAGK"
     gene            complement(3680..>4132)
                     /locus_tag="ACZPCG_12085"
     CDS             complement(3680..>4132)
                     /locus_tag="ACZPCG_12085"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1285822.1"
                     /translation="DDEAREVAMKYIKMVQLEDFVDAYPKELSGGMKQRVAIARAYAV
                     QPEVLLMDEPFGALDAQTRTQLQSELIKTWQEEKKTCFFITHDVEEAVILATKVIIMS
                     ARPGRIKKIVDINLPYPRTQEMKMELPFLDLKTYIWGEVYKEYLEVRK"
BASE COUNT          939 a         1214 c          838 g         1141 t
ORIGIN      
        1 ctcctcctcc cgctgcaagg atccctgcct tatcctttcg ttcctcacag tggccgatga
       61 gcacataact gcatcccaaa gctctggctg cattagctgt gcggctggta gtaaaggctc
      121 cgaagttccc ccccactgcc gtatcctggc ggaatactcc ctggcagcct acctttaccg
      181 gactgccttc gcagagagtc cctgcagctg tgagtatgtg ggcttcgggg aaaaacatgg
      241 caaattccac atcctctgtt ccatactcca taagcccctt ctgagtctgc tccacaatat
      301 aggaacccca gtccttcacg gcagcaatag cgtttacgcc gccatactcc tttggaacat
      361 caaaacgctt cagatttaaa aagatatgct tcattctctg tcctctcttc tttccctgta
      421 tatctcctga aaatcaatat agtccggtat ttcctctccg gtaagagggc ggcaccagct
      481 tacaaaggca tccgtcacat catttcccct gttattgata taggaagccg gaacagtccg
      541 ttctgtaagc atcacctgtt ccaccggaat acggatgggg cagacctcat atttctcctg
      601 ctccctgcgc gcaaagccga tcataatgcc tgtacagccc tccattgcag cctttaaggc
      661 ttctcttcct gcaagcaccg cctcctcacg gtccaccggg gactgaaggg agatggatgc
      721 tctgccgcaa agccccggct tttcgcttct ggccttaatt cccagatttt tgatcaccag
      781 ctctgccaga taagctccca cgtctccgta gtagacggaa cggcctgtct ggaaaatggg
      841 aggcacgaca ggctttccgc tttcatcctt taagccctcg ctggcgacca caaccacacc
      901 gcccagcctg tcatacagct ccttcacatc tgccagaaat tcctcttccc tgaaaggacg
      961 ctccggaaga tagatcaggt gcggcgcatc cccgtgtttt ttcctcgcca gagctgaggc
     1021 cgctgtaatc cagcctgcat tgcgccccat ggcctctaaa atacacacat gaatgggaag
     1081 agatttcaca tccgccccta tctcagccgt tgttctggct atataccggg ccgtgctgcc
     1141 gtagcccgga gtatgatctg taatgccgat atcattatca atggtctttg ggattcctgc
     1201 cacccggata tctgagtctg gacctaaagc cctcaccagc tttccgcagg cgtccatcgt
     1261 gccgtttccc ccgttaaaaa gcacatatcg gatatggtgc ttacggaaaa tggcctccat
     1321 tgccttataa tcttcctcct ccagcgcata tcgggaagag ccaatggcag ttcccggcgt
     1381 ggtaaggagc tttttcagct cttcttcagg aaaccgggac agatttaaaa accgttcctt
     1441 taaaattccc tcgcttccgc cgatggctcc gtaaactgcg cccactgcgc cgctttccct
     1501 tgcttcaaga agtacgccgt acagcgatgc attcagcact gccgtaggcc ctcctccatg
     1561 aacaaccagt acattgtccc tcataaacca acctgccttt cttctcagtc agacagagct
     1621 gccacataat ccgtaaaggt aagggtaaga tccgggtggc tctggagcag gcttacggga
     1681 aaatcagagg acggttcacc gtatgcggcc ctccgcacca ctgcccgatg ccagtttcta
     1741 aagcagccca gacggatttt tctcgcatga gcgatctcat aaataccaat ggttacgcag
     1801 taatgaggca tatcctcaag agctccgtta aaatctccga tggcattggc cgtccgtgtt
     1861 tcaggggtaa ttgcaagcac acgggtcttc tgagccagaa atccctcatt ggtcagcgac
     1921 gcatctgctt cattaaaagc cacatggccg ttaatgccga ttcctccaaa ggcaatatcc
     1981 acgcctccca gctcttcaat cagttttggg acccgttccg tatcaaaagg atccggaaat
     2041 acccgctgtt ctttgggcat cacaagctct gggcgtatcc tgtcatatac ggtacggttc
     2101 ataaagcccc gaaagctcag aggatgatcc tccggcaccc agcgcttctc atcatccaga
     2161 tattcgtcca tattgataaa ccatacattt ttaagactga tattctgctc atttaccaga
     2221 tccacaaaat acggatactg tcccaccggc cctaccggac agataaagac ggtgtgcttt
     2281 ccctctgcat tatgagcttt gatctcttct gccatctgct ctgccatctg tttaaataca
     2341 gcagcattat cctccaagca aaacagcctc atttttggat tttccaaaag ctgctctttt
     2401 gtatagctgt aatagtcgtg tttcataact gctcctttcc ctgtcacgcc ggcccttctg
     2461 ccatttttcc gccataaaga gagcgtctgg cgccccaacc ggcccccatg acgctccctg
     2521 caatgatttt tacttacctg cttcgatcat attgtcaagc attacataat ctgctacagg
     2581 aacttccttt tccacctgtc ctccggcaat gaaattctcc tgctggatct tgtagtaatt
     2641 ctctacggtt ccgtctgctg caccttctga tacttccttt ccggtcagcc agtctgcatc
     2701 gcctctctgg tcaaatacgc ttgcttcatc tgtctttgta atatcggcaa ctgcctttgc
     2761 agtctcctca tagtgatcgt ctgccgcata atccattcct ttaaagagcg cttttgtaaa
     2821 gcgaaggatg gtatcctgat tctcctctgc atactttgga gttacgatcc agcttgccag
     2881 agaaacggta gtatccttaa aggtcatatt atcagccagc ttggttgcct ccggaatctc
     2941 ttccatgatc tttaagctgt ttggagacca ggtagcacag gcatctacgc cgcctgaaat
     3001 cattgccgta acaataccgg atgcgtccat atcaacggcc tggatatcgc tcatggtcat
     3061 accggccttt gtaagtgtat tatttaaaat atcctcactg gatgtaccgg aggaataagc
     3121 caccttctta ccctttaaat cttccatggt cttcacactt gatccgccga tcacagcgtc
     3181 gccgttggaa atatgggaca atgcaaaaat cttggcctgg ccgttgacac aaagcttgtg
     3241 tgcgccctgt ccgatatagc cgatatcaat actgccggac tccatcgccg caatgatggt
     3301 agggccatct gcaaaggata ccagattcag cttaattcct tcttcttcca tgaatccctg
     3361 agcctgtgct gccgccacag accaaaggct tccatagttt ggcatatatg caatatttaa
     3421 tgtaatgggc tctgcctctg tcttttcctc ttctgccttt gtctcctttt cagtttcagc
     3481 cttggacgtt tcctctgcct tggatgcctc cgcagactcc ggagcctttg tctcaggagc
     3541 agaacttccg ccggagcagg ctgtcacgga caatgccatt actgctgcca ttgctgctgc
     3601 taaaaaacgt tttttcatag atttgtatcc tccttaaaat aaatgatata gttttgaaac
     3661 acttaccatt ttgctcctat tactttctta cttccagata ctccttatat acttcacccc
     3721 agatatacgt cttaagatcc aggaacggaa gctccatctt catttcctgt gttctcggat
     3781 acggaaggtt gatatctacg atctttttga tacggcccgg ccttgcactc ataataataa
     3841 ccttggttgc aaggataaca gcctcctcta catcatgtgt aataaagaaa caggtctttt
     3901 tctcctcctg ccacgttttg atcagctctg actgaagctg cgtccttgtc tgggcgtcta
     3961 aggctccgaa gggttcatcc atcaaaagca cctccggctg taccgcatag gctctggcaa
     4021 tagctaccct ctgcttcatg ccgccggaaa gttcctttgg ataagcatcc acaaaatcct
     4081 ccagctgtac catctttatg tacttcatgg caacctctct tgcctcgtca tc
//
LOCUS       DBMDUH010000186         4107 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_7876_length_4107_cov_6.710185, whole genome shotgun sequence.
ACCESSION   DBMDUH010000186 DBMDUH010000000
VERSION     DBMDUH010000186.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4107)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7876_length_4107_cov_6.710185"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            241..1758
                     /gene="yfcC"
                     /locus_tag="ACZPCG_12090"
     CDS             241..1758
                     /gene="yfcC"
                     /locus_tag="ACZPCG_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003506942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HIR1285823.1"
                     /translation="MEKKKRSFQMPHTYIIIFGVILVAAILTLFIPLGQYDTKAITYT
                     VNGEEKTRTVVDPDSFRYVLDENGDKVTQAAPLFGTESVGGRGGILNYVFEGLTTGDK
                     SSGAVGIIAFILVVGGAFGVVLRTGAIESGIMRVIDLTNGKEILLIPILITLFSLGGA
                     VFGMGEEAIPFVMILVPMFIAMGYDAIVGIMCSYVATQIGFGSSWQNPFGLAVAQGIA
                     GVPVMSGAWFRIPLWIFFTVLTIVFTMRYAARIKKNPKLSAAYESDQEYREEFAKRGR
                     DELPFTLGHKLVILDVFLCIIWTIWGVVEKGYYIPEIASQFFVMGLAAGIIGVAFHLN
                     DMRLNDIPRAFDHGAADLLGAALCVGMAQGIIIILGGTDPSQGTVLNTILHTLSDGMK
                     NFPPVISAWFMYLFQSVFNFFVVSGSGQAALTMPIMAPLADLVGVERQVAVVAFQLGD
                     AFTNFIVPTSGCLLGALAAARLEWGQWAKFQIRFQALLAACGSAAVILGVLTGLS"
     gene            1780..2100
                     /locus_tag="ACZPCG_12095"
     CDS             1780..2100
                     /locus_tag="ACZPCG_12095"
                     /inference="COORDINATES: protein motif:HMM:NF017920.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HIR1285824.1"
                     /translation="MELLIIIVQELDFPRLSSAFIHNHVLATKFCTEGLYLNKRNITL
                     MVCIPKEREEEILGYIRSSCIEREEERLVSEFNGMTMVDVTKGVKVGGATVFFIDIDR
                     MQKF"
     gene            2172..3224
                     /locus_tag="ACZPCG_12100"
     CDS             2172..3224
                     /locus_tag="ACZPCG_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003509988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HIR1285825.1"
                     /translation="MDLSFAFFWKQVLGSPALMITVFLTLCVIFVNGWTDAPNAIATC
                     VSTRSMDVELAIIMAAVCNFAGVMVMTMVNSTVAMTITNMVNFGGDNHRALIALCAAL
                     FAIVAWAVLAWYFGIPTSESHALIAGLSGAAIAMQGGIWGINGSEWIKVIYGLGLSTV
                     LGFVLGFFVCKLVSVLCGRFDRRRTDGFFKGAQIGGAAAMAFMHGAQDGQKFLGVLLL
                     GICLSGGNDNAAGIRLPVWMMLLCSLIMGTGTSIGGKKIIKSVGMDMVKLEKYQGFSA
                     DLAGALCLLLSSVFGIPVSTTHTKTTAVMGVGAVKRLSAVNFQVVREMMLTWVLTFPG
                     CGVIGFLMAKLFMVLF"
     gene            3251..3874
                     /locus_tag="ACZPCG_12105"
     CDS             3251..3874
                     /locus_tag="ACZPCG_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008720979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HIR1285826.1"
                     /translation="MSKKSDNYYFKNFVICAEYGEEAARMLERNLKEFDVTHLSERLD
                     EIHGIEHAADQKKHVMLAALVKEFITPIEREDIILLSEAIDEVTDTIEDVMIRLYINN
                     IRMIRPDAEAFAHLITRCCKTLSRLMEEFKNFKKSKKLYGLMVELHTLEGEGDRLFIQ
                     AMRRLHTEGSDSLEVIVWREIYIYLEKCCDACEHTANAAERVILKNT"
BASE COUNT         1043 a          749 c         1164 g         1151 t
ORIGIN      
        1 aatgcatgga gctgactgat actaatcggt cgagggcttg accagaaggt ttagggaaaa
       61 cggttgtggc ttggttgttc agtatgtggt tttgaaggta tgtatgaatg agtgtgagac
      121 cgcctgaaag ggcggttttt tcgtgtgtaa aaacatataa acagaaaatg ctccttgatg
      181 aaataaggcg ttgagtgata taattgttac tgctgaaaat gaaatttatg gaggaaataa
      241 atggagaaaa agaaacgatc ttttcagatg ccacacacct atattattat ttttggggtg
      301 attcttgtgg cggcaatttt gacgcttttt atccctttgg gacagtatga cacaaaggcg
      361 attacctaca ctgtgaatgg ggaggaaaag acccgaacag tagtggatcc ggacagcttc
      421 cgttatgtac tggatgaaaa cggagataaa gtcacccagg cagcacctct ttttggcaca
      481 gagagtgtag gaggccgggg aggtatcctg aactatgttt ttgaaggcct taccacagga
      541 gataagagtt ccggcgctgt gggaatcatc gcatttatcc tggtggtagg aggtgccttt
      601 ggcgttgttc tgcggacagg tgccattgaa agcggcatta tgcgtgtgat cgatctgacg
      661 aatggaaaag aaattcttct gattccgatt ctgattaccc tgttttcact gggaggagca
      721 gtttttggaa tgggagaaga ggcaattccc tttgtaatga tcctagttcc catgtttatt
      781 gctatgggat atgatgcgat cgtaggcatc atgtgcagct atgtggcaac tcagattggc
      841 tttggcagct cctggcagaa tccatttggt ctggctgtgg cacagggaat tgccggagtt
      901 ccggttatgt ccggcgcatg gtttcgtatc ccgctgtgga tcttttttac agtccttaca
      961 attgtattta ccatgcggta cgcggcaagg ataaagaaaa atccgaaatt atctgcagcc
     1021 tatgagtccg atcaggagta tcgcgaggaa tttgcaaaac ggggcagaga tgagctgccc
     1081 ttcactctgg ggcacaagct ggtcatcctg gatgtatttt tatgtattat ctggaccatc
     1141 tggggcgtag tggaaaaggg gtattatatt ccggagatag cctcccagtt ttttgtaatg
     1201 ggccttgcgg caggcatcat tggcgttgca tttcacttaa atgatatgag gctgaatgat
     1261 attccaagag cctttgacca tggagcggca gatcttctgg gagcggctct ttgtgtaggt
     1321 atggcgcagg ggatcattat tattctgggt ggtacagatc cgtctcaggg aaccgttctc
     1381 aatactattc ttcatacgct cagtgatggg atgaagaatt tcccgccggt gatttctgcc
     1441 tggtttatgt atctgttcca gtcggtgttc aacttctttg ttgtttccgg ttccgggcag
     1501 gctgcgctca ctatgccgat catggcgcca ttagcagatc tggtaggcgt ggagcgtcag
     1561 gtggcagtcg ttgccttcca gctgggcgat gcctttacga attttattgt tccgacatcg
     1621 ggctgtctgt tgggagccct tgccgcagcc aggctggaat ggggccagtg ggcgaaattt
     1681 cagatccgtt ttcaggcgtt gcttgcagcc tgcggttctg cagctgtgat tcttggtgtg
     1741 cttacaggtc tgtcataaga cagggaaagg agagcgggta tggagctttt gatcattatt
     1801 gttcaggaat tggattttcc caggctgtcc agtgcattta tccacaatca tgtactggca
     1861 actaaattct gtacagaagg actgtatctg aataaacgaa atattacttt gatggtatgt
     1921 attccaaaag aaagagaaga ggaaatactg ggctatatcc gcagcagctg tatcgagcgg
     1981 gaagaggagc gcctggtcag tgaatttaac ggtatgacaa tggtggatgt gacaaaagga
     2041 gtaaaggtgg gcggagctac cgtatttttt atagatattg accggatgca gaagttttaa
     2101 tctccgtgcc tttacatcat atgcaggtcg tgataagatg aaactgaaat gtgtaaaagg
     2161 atggaactta catggatctt tcattcgcct ttttctggaa acaggttctt ggaagtccgg
     2221 ctttaatgat cactgttttt ctgaccctgt gtgtgatctt cgtaaatggg tggacggatg
     2281 cccccaatgc cattgcgacc tgtgtttcta cacggtctat ggatgtggag ctggcgatca
     2341 tcatggcagc tgtctgcaat tttgccggag taatggtgat gactatggta aattctaccg
     2401 tggccatgac cattacaaat atggtaaatt ttgggggaga taatcatcgg gctcttatag
     2461 ctctctgcgc ggctctgttt gccattgtgg catgggcggt gctggcctgg tatttcggca
     2521 ttcctaccag tgagagccac gctttgatcg cagggctctc aggcgctgcc attgcaatgc
     2581 agggcggcat ttggggcatc aatggcagtg aatggatcaa ggtcatctac ggactgggac
     2641 tttcgacggt tttggggttt gttctgggct tttttgtctg caagctggta tccgttttgt
     2701 gcgggagatt cgaccggaga aggacagacg gtttttttaa gggagctcag atcggaggag
     2761 cggcagctat ggcttttatg catggcgctc aggatgggca gaagtttctg ggggtactgc
     2821 ttcttggaat ctgtctgtcc ggaggaaatg acaatgcagc agggatccgg cttcctgtat
     2881 ggatgatgct cctctgttct ctgatcatgg ggacaggaac ctcgattggg ggaaaaaaga
     2941 ttattaaatc agtagggatg gatatggtaa agctggaaaa atatcaggga ttttctgctg
     3001 atctggctgg agcgctgtgc ctgcttttat cctctgtgtt tgggattcct gtgagtacca
     3061 ctcatacaaa gacaacggct gtcatgggcg tgggagcggt aaagcggctg tcggctgtca
     3121 attttcaggt cgtaagggaa atgatgctga cgtgggtact gacctttcca ggctgcggag
     3181 tgatcgggtt tctgatggcg aagcttttta tggtattgtt ttaggaaaag cagaatgctt
     3241 gggagggaaa atgtcaaaaa aaagcgacaa ttattatttt aagaatttcg tgatctgtgc
     3301 agagtacggg gaggaggctg cccgtatgct ggaacggaat ttaaaagagt ttgacgtgac
     3361 ccatttatca gaaaggctgg atgaaatcca tggaatcgaa catgccgcag atcagaaaaa
     3421 gcatgtgatg ctggcggccc tggtaaagga atttatcaca ccgatcgaac gggaggatat
     3481 tatcctgctc agtgaggcaa tcgatgaggt gaccgatacg attgaggatg tgatgatccg
     3541 gctttatatc aacaatatcc gtatgatccg tccggatgcg gaagcatttg cccatttgat
     3601 cacacgctgc tgcaaaacct taagcaggct tatggaggaa tttaaaaact ttaaaaagtc
     3661 aaaaaagctt tatggactta tggtggaact ccatacactg gaaggagagg gagaccgcct
     3721 ttttatacag gctatgcgca ggctgcatac agagggttcg gattctctgg aggtaattgt
     3781 ctggagagaa atctatattt atctggaaaa atgctgtgat gcctgtgaac atacggcaaa
     3841 tgcggcagaa cgtgtcatac tgaagaacac ctgatacacg gcagaaagag cagaagcgga
     3901 agtttggtaa aatttactta agagagagaa atatttggta aacggatttg aacggaggaa
     3961 aaaatgcctg ccggtcagat ccgttttttg ctgttatata ttggaaaagc gtagaaaata
     4021 gtcggatttt tgaaatgacc gcggggataa caaaagaagg taaacacaaa acctggaaga
     4081 tgaatggtaa accaatgggg agaaatt
//
LOCUS       DBMDUH010000187         4037 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_8024_length_4037_cov_4.084604, whole genome shotgun sequence.
ACCESSION   DBMDUH010000187 DBMDUH010000000
VERSION     DBMDUH010000187.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4037)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8024_length_4037_cov_4.084604"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            155..2023
                     /locus_tag="ACZPCG_12110"
     CDS             155..2023
                     /locus_tag="ACZPCG_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285827.1"
                     /translation="MGNFGWHTESDHGRVYSLKDLEMTSYKREGRTFRYPVKKTEKNE
                     KVYDWLRKNPHKINLYRLGFRVNGRRISSGQITEVHQELDLYTGILNSSFQVDKEAVK
                     VQTVCSGNSNRLGLRIEAGESLKKNMSLELEFPYGSPDITGSDWTSPSRHGSYLVSLG
                     EKRYLINRVMDDLLYQVMIELDGAQMIQEEEHRFSIRFDAAKAEITILTDRAVPRQFA
                     TFNKCCEESEVFWRDYWENTGLIDFGETQDTRAHELERREILSLYLLRLQCAGSLPPA
                     ETGLSCNSWYGKFHLEMHFWHSAFLPAYSQGRLLEKSFEWYIEHRDEAKENAERNGYK
                     GIRWPKMVGCDGEDSPSPIAPLLIWQQPHIIFMLELLYQSEPSEELVEKYYGIVRETA
                     DFMADFAEWNSEKNVYELRPPLIPVQETHDPETTLNPVFELEYWREALNIADEWAKRS
                     GRKGNSRWGMVSEAMALPASAEGVYLTHENGADTYINHAKDHPSMLMACGILRGDRLD
                     RETVERTLQKVLEVWDFNTAWGWDFAVMAMTAVRLDLGDLAVDILLKDTQKNEYVLNG
                     HNKQGYRKDLPLYLPGNGSFLYALAMMAAGYRGCGKKMPGFPENWTVRAEGIKALY"
     gene            2072..3448
                     /locus_tag="ACZPCG_12115"
     CDS             2072..3448
                     /locus_tag="ACZPCG_12115"
                     /inference="COORDINATES: protein motif:HMM:NF024808.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1285828.1"
                     /translation="MRKLLKKSMAAVLTASMAMSMTACAGKAVKPGSEESEAKTSEAA
                     AANVETSEVAGEEGLTTEDITLTISWWGGESRHEATQKAIEAFMKKYPNIKVESSFGG
                     WTGWESKMGLAFSQGKADDIVQMGPFWHTMFGGDGEVFYDLNQLSDYLDLSQFENLEP
                     YTVDGELRAVPVSMAARVMFWNKDTFDQAGVPIPKTWDDLLASGAAFKEKLGDEYYPM
                     MLNTLDRSLFMVWYLESKYGKEWVTGNQCNYTEEEIAEGFEMIQSLEEAHVIPTLQMI
                     ADNAANPVEQSPVWTGGKWAGVYTWNTSSANPKAALPNPEAMVTSHMFEGFPHKGGMF
                     RASMTFGITKTCKYPKEAAALLEFMLNDPEGVALMANERGIPYSKAGLEALEASGNVD
                     ELTKMATEYALEDNTFPMDEYFECSEYKATTEGLYDKVFNAFAYGETDAKGAAKMLLD
                     GMNAKMSK"
     gene            3689..>4037
                     /locus_tag="ACZPCG_12120"
     CDS             3689..>4037
                     /locus_tag="ACZPCG_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008755321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1285829.1"
                     /translation="MNLQKSSYWYTLDNYRQIFTDPQMLKVLTITFKYAFLTVPLKLT
                     AALFIAYILCFNLKGVRFFRTAYYIPSVLGGSIAVAVLWKALFQKQGVINNLLMVLGI
                     EGPSWLGDPGMLGR"
BASE COUNT         1190 a          714 c         1086 g         1047 t
ORIGIN      
        1 atataaattg ataaaaaaac acaacataat attgtacgaa ccagcatatg attctccgct
       61 ttccgtgggg aatggagagt tcgtttttac agcggatgta acgggagttc aaacattgta
      121 tgaagagtat agagatgcat ttcctctctg tacaatggga aattttggat ggcatacaga
      181 gagtgaccat ggacgtgtgt acagtcttaa agatctggag atgacttcct ataaacgcga
      241 aggacgtaca tttcgctatc ctgtgaaaaa aacggagaag aacgagaagg tttatgactg
      301 gctgagaaaa aatcctcata agataaatct ttaccgtttg gggttccggg taaatggacg
      361 aaggatttcc tcggggcaga ttacagaggt ccatcaggaa ctggatttgt atacgggtat
      421 tttaaacagt tcgtttcaag ttgataagga agcggtaaag gtacagactg tatgcagcgg
      481 aaacagcaac cggcttggac tgaggataga agcaggggaa tcgttgaaaa aaaatatgag
      541 cctggaactg gaatttccct acggttctcc ggatataacc ggttcggact ggacttctcc
      601 atccagacat ggaagctatc tggtttctct tggagaaaaa cggtatctga taaatcgtgt
      661 tatggatgat ttactgtatc aggttatgat agaactggat ggagcgcaga tgatacagga
      721 ggaggaacat cgtttctcaa ttcgatttga tgcagcaaaa gcggagatca ctattttaac
      781 tgaccgcgct gtgcccaggc agttcgcgac atttaataag tgctgtgaag aaagcgaagt
      841 gttttggagg gattactggg aaaataccgg cctgattgat tttggggaaa cacaggatac
      901 cagagctcat gaactggagc gcagggaaat tctttcattg tatcttcttc ggctgcaatg
      961 cgcaggaagc cttccgccgg ctgaaacggg actgagctgc aacagctggt acggtaagtt
     1021 tcatcttgag atgcattttt ggcatagcgc ttttctcccg gcctattctc aggggagact
     1081 gcttgaaaaa tcctttgaat ggtacataga gcaccgggat gaagcaaaag aaaatgcgga
     1141 gagaaacgga tataagggga tacgctggcc caagatggta ggctgtgatg gagaagattc
     1201 gccatctccg attgctccgc tgttgatctg gcagcagccc cacattattt ttatgctgga
     1261 attgctttat cagtcagaac catctgaaga gctggtggaa aaatattatg gaattgtcag
     1321 agaaaccgct gattttatgg cagattttgc agaatggaac agtgagaaaa acgtatatga
     1381 gctgcggcct ccgctgattc cggtacagga aactcacgat ccggaaacaa ctcttaatcc
     1441 ggtatttgaa ttggaatact ggagagaagc tttgaatatt gctgatgaat gggcaaagcg
     1501 ttctggaaga aaggggaaca gcagatgggg gatggtaagc gaggctatgg cgcttccggc
     1561 atctgcggag ggagtttatc ttacccatga gaatggtgcc gatacttata taaatcacgc
     1621 aaaggatcat ccgtccatgc tgatggcctg tggaatactc aggggcgacc gcctggatcg
     1681 ggaaacggta gaacggacgc ttcagaaggt tctcgaggtc tgggatttta acactgcctg
     1741 ggggtgggat tttgccgtaa tggcaatgac agccgttcgt ctggatctgg gggatctggc
     1801 tgtagatatt cttttgaaag atacacagaa aaatgaatat gtattaaacg gtcacaataa
     1861 acagggatac aggaaggatc ttcctttgta ccttccgggt aatggatcat ttctctatgc
     1921 tctggccatg atggcagccg gttacagagg ctgtggaaaa aaaatgccgg gttttcctga
     1981 aaactggaca gtacgggcag aagggattaa agcgttgtat taaatgcgga atcagaatgt
     2041 ccgcaaagag ataaagcagg aggttatttc aatgagaaaa ttattaaaga aatctatggc
     2101 agcagtatta actgcatcaa tggcaatgtc tatgacagcc tgtgccggta aggcggtaaa
     2161 gccgggaagc gaagagagtg aggcaaagac cagtgaggct gcggcagcaa atgtggagac
     2221 gtcggaagta gcaggggaag agggacttac cacagaggac ataaccctta ctatcagctg
     2281 gtggggagga gaaagccgtc atgaagctac gcagaaggca atcgaggcat tcatgaaaaa
     2341 atatcccaat attaaggtgg aatcaagctt tggcggatgg actggttggg aatctaaaat
     2401 gggactggcg ttttctcagg gaaaggcaga cgatattgtg cagatggggc cgttctggca
     2461 tactatgttt ggcggagatg gagaagtttt ctacgactta aatcagctgt ccgattatct
     2521 ggatttgagc cagtttgaaa accttgaacc ctatacagta gacggggaac tgagggcggt
     2581 tccggtttcc atggcagcca gagtgatgtt ctggaataaa gatacgtttg atcaggcagg
     2641 agttcctatt ccgaaaacat gggatgatct tttggcatcc ggcgctgcct ttaaagaaaa
     2701 gctgggtgat gaatattatc caatgatgct taatacgttg gatcgctctt tattcatggt
     2761 atggtatctt gagtcaaagt atggaaaaga gtgggtaaca ggcaatcagt gcaattatac
     2821 cgaggaggaa attgcagaag gctttgagat gattcagtct ctggaggaag cccatgtaat
     2881 tcccactctg cagatgattg cagataatgc agccaatcct gttgagcagt cgccagtatg
     2941 gacaggggga aaatgggcag gcgtatacac atggaataca tcctctgcaa atcccaaggc
     3001 tgctcttcct aatccggagg ctatggtaac ttcccatatg tttgaaggat tcccgcataa
     3061 aggcggtatg ttcagagcat ctatgacatt cggaattacc aaaacctgta aatatcccaa
     3121 ggaagcagcc gctcttcttg agttcatgtt aaacgatcct gagggtgtag ctcttatggc
     3181 aaatgaaaga ggaatccctt attcgaaagc aggtctggag gctttggagg catctggaaa
     3241 tgtggatgag ctgactaaaa tggcaacaga gtatgctctt gaagataata cattcccgat
     3301 ggatgagtat ttcgagtgca gcgagtacaa ggcgacaaca gaaggcttat acgataaagt
     3361 atttaatgca tttgcatatg gagagacaga tgctaagggg gcagctaaaa tgcttctgga
     3421 tggcatgaat gccaaaatgt ccaaatagta cagaataaaa ccgggtatta tgcaggtgtt
     3481 ggtggcacat caatcatatg atgtgccacc atgcataatt aagaggagga tcggtgtgaa
     3541 aatacagaaa cagaacaaaa gtttgtattc tgccagggaa agaagaagga tgaggatcgg
     3601 atttttgtat gcagtcccct ggctgattgg ttttttggtg tttaagggat atccgttttt
     3661 atcatcttta tattacagtt ttacagatat gaatttgcag aaaagctcat attggtacac
     3721 tctggacaat tacaggcaga tatttacaga ccctcagatg ctgaaggttt taaccataac
     3781 ctttaaatat gcatttttga cagtgccatt aaaactgacg gcagcattgt ttatagcgta
     3841 tattctttgc ttcaatttaa aaggggtaag attttttcga accgcttact atattccgtc
     3901 tgttttaggc ggttctattg cagtagctgt attatggaaa gcactgtttc agaaacaggg
     3961 cgtaattaat aatctgctga tggttttggg gattgaaggg ccatcatggc tgggagatcc
     4021 gggaatgctg ggccgtc
//
LOCUS       DBMDUH010000188         4000 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_8113_length_4000_cov_6.452937, whole genome shotgun sequence.
ACCESSION   DBMDUH010000188 DBMDUH010000000
VERSION     DBMDUH010000188.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4000)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8113_length_4000_cov_6.452937"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="ACZPCG_12125"
     CDS             <1..914
                     /locus_tag="ACZPCG_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HIR1285830.1"
                     /translation="HPAVLSYLDIRPQYFCQVETTVDGKRFVTPRGWEDLSQFITVYE
                     NLDKRADREVAGQYLQHPAVAKDFANYLELYYKYQNEYQVEEILKGVIREEVCTRLER
                     APFDERLSVVDLLLAKLTDGFRELYENKAEMELLMARLKEFKRELDCSQDVVPAVRLA
                     SVAEETELTRIHRKDSGLSDRREDRRYRRVAARLESWVQEMKGASLNEAEAAWQWTRE
                     QFMERSDSYENQKEACAKALEHAFDFMETAFGNGQEMVIFITSLNTGSCSIEFLKEYE
                     CERYYHYNKELLFDEQEADVRRLLEKK"
     gene            932..2611
                     /locus_tag="ACZPCG_12130"
     CDS             932..2611
                     /locus_tag="ACZPCG_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1285831.1"
                     /translation="MKVLIVGGVAGGAGTAARLRRNDENAQIIMFERGQYISYANCGL
                     PYYVGGVITDKGALQLQTPERFHQRFNVDVRTESEVTAVDTAGKTVTVRHGEETYKES
                     YDRLVLSPGAAPIRPAIPGIEENKVFTLRNIPDTYRIYDHIKETAPRSCAVIGAGFIG
                     LEMAENLAERGVRVTVVEGASHVMPPIDMDMAHQVHNYIRAQGIDLYLGQTCTAMDKD
                     GVILKDGRKIPADMVILSIGVRPDTGFLKDSGIELGARGEILVNSYMETSAPDVYALG
                     DAASVRHIVSGKQVLIPLASPANKQGRIVGDNLCGRKTAYKGSQGTSIMKFFGLTVAV
                     TGEKEESLQAQGRAFCKVITTSASQAGYYPGGEMMTVKTLFEPDTGRILGAQIVGGKG
                     VDKRIDDMANAVRFGLTGFDLQEMELAYAPPFSSAKDPVNMAGYVIGNVIESLMKPFY
                     VEDLDQIPEDAVRLDVRTPEECQRGMIPGFINIPLDSLRERMGELDREKDIYITCQIG
                     LRGYLAQRILDQAGFKTWNLSGGYRLYEMVKKDEAAMAAVAGMCTECGKEL"
     gene            2706..3422
                     /locus_tag="ACZPCG_12135"
     CDS             2706..3422
                     /locus_tag="ACZPCG_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR1285832.1"
                     /translation="MKITYIHHSSFLVEMEQIALLFDYFEGEIPEIRQDQLLIVFASH
                     RHGDHFSPGIFDLAENHPKIWFVLSDDIWRKRVPEEFLCRTSFLGPGEEFSFQKETGE
                     KVPVTVKAFKSTDEGVAFLIEAEGKVLYHAGDLNNWVWEGEPEEYNCRMSEAFHSELL
                     KIAGNHIDVAFMLLDPRQGKDFYLGMDDFMHMVGADIVFPMHFWGDFGVTERFKTLPC
                     AADYAEKIMEIRCQGESFQL"
     gene            3458..>4000
                     /locus_tag="ACZPCG_12140"
     CDS             3458..>4000
                     /locus_tag="ACZPCG_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR1285833.1"
                     /translation="MILIKGSRVLDPAGKTDSRADLVIEDGIIREICPPDTLGGRTEA
                     EYEQIIDGTGLVTAPGLVDVHVHFRDPGLTYKEDIHTGAAAAAKGGFTTVVCMANTKP
                     LVDNEETLRYVLEEGKKTGIHVLSCAAVSKGFKGEELTDMEGLLAAGAAGFTDDGIPL
                     MDGPFVLKAMEEAARLDTVIS"
BASE COUNT         1106 a          737 c         1179 g          978 t
ORIGIN      
        1 tccatccggc cgttttatcc tatctggaca tccgtcccca gtatttctgc caggtagaga
       61 ccaccgtgga tggaaagcgg tttgttacgc ccagaggctg ggaagatctg tcccagttca
      121 ttacagtcta tgaaaatctg gataaaaggg cagatcggga agtggcaggg cagtatctcc
      181 agcatccggc ggttgcaaag gattttgcca attacctgga actgtattat aaatatcaga
      241 atgagtatca ggtagaggaa atcttaaaag gagtgatccg ggaagaggta tgtacaaggc
      301 tggagcgggc gccttttgat gagcgcctca gcgttgtgga tctgctcctt gcaaagctga
      361 cggacggttt ccgggagctt tatgaaaata aggcggaaat ggaacttttg atggcccgtt
      421 taaaggaatt taaacgggag ttggattgtt ctcaggatgt agtcccggct gtgcgtctgg
      481 ccagtgtggc ggaagagaca gagcttacca gaatccacag aaaggacagc gggctgtcag
      541 accggagaga ggacaggcgg taccgcagag tggctgcccg gttagaaagc tgggttcagg
      601 agatgaaggg agcttcttta aatgaggcag aagcggcctg gcagtggacc agagagcagt
      661 ttatggagcg cagcgacagc tatgagaatc agaaggaggc ctgcgcgaaa gcattggaac
      721 acgcatttga ctttatggaa acggcctttg gaaatggtca ggagatggtt atttttatta
      781 cctcattaaa tacaggttct tgcagcatag agtttttaaa ggaatatgag tgcgaacggt
      841 attaccatta taataaagaa ctgttgtttg atgaacagga ggcagacgtg agacggcttt
      901 tggagaaaaa ataaaaaagg aggtattcta tatgaaggta ttgatcgtag gaggcgtggc
      961 aggaggagca ggcacagcag ccagacttcg cagaaatgat gaaaacgcac agattatcat
     1021 gtttgaacgg gggcagtata tttcctatgc gaactgtggc cttccttact atgtgggagg
     1081 agtgatcaca gataaggggg ctttgcagct gcagacaccg gagcgttttc atcagagatt
     1141 taatgtggac gtgaggacag agagtgaggt tacagcggta gatacagcag gtaagacggt
     1201 aacagtacgt catggggagg aaacctataa ggaaagctat gaccgtctgg tgctgtctcc
     1261 cggtgcagct cccatccgtc ctgcaattcc cggaatcgag gaaaataagg tatttaccct
     1321 gagaaatatt cccgatacct atcgtatcta cgaccacata aaggaaactg cgcccagaag
     1381 ctgtgcggtg atcggagcag gctttatcgg tctggaaatg gcagaaaatc ttgcagaacg
     1441 aggtgtgcgt gtaacagttg tagagggtgc ttctcatgtg atgcccccca ttgatatgga
     1501 tatggctcat caggtgcata attatatccg cgcgcaggga attgacctct atctgggcca
     1561 gacctgtact gctatggaca aggatggagt aatcttaaag gacggaagga aaatcccggc
     1621 tgatatggtt atcttaagta taggagtacg tcctgatacc ggatttttaa aggacagcgg
     1681 aattgaattg ggagccagag gggaaatcct ggtaaattcc tacatggaaa ccagtgcgcc
     1741 tgatgtctat gctctgggtg atgcagccag tgtacgtcat atcgtaagcg gaaaacaggt
     1801 cctgattccg ctggcttccc cggccaataa gcaggggcgt attgttggag ataatctctg
     1861 tggaagaaaa acggcttata agggcagtca gggtacctcc atcatgaaat ttttcggtct
     1921 tacggtggct gtgacaggtg aaaaggaaga aagcctgcag gctcagggac gtgctttctg
     1981 caaggtaatc accacctctg cttctcaggc tggatattat cccggcggtg agatgatgac
     2041 agtaaagacc ttgtttgagc cggatacagg ccgtattctg ggtgcacaga ttgtaggcgg
     2101 aaagggtgta gacaaacgca ttgatgatat ggcaaatgca gttcgctttg gccttacagg
     2161 ctttgatctg caggagatgg agttagcgta tgctccgcca ttctcatcag caaaggatcc
     2221 ggtaaatatg gctggatatg taattggaaa cgtaatagag agccttatga aaccgttcta
     2281 tgtggaagat ctggatcaga tcccggaaga tgcagtgcgc ctggatgtgc gtaccccgga
     2341 agagtgccag agaggtatga taccagggtt tatcaatatt cctctggatt ccttaagaga
     2401 acgcatgggt gagcttgatc gggaaaagga tatatatatc acctgccaga tcggcttaag
     2461 aggttatctg gcccagagaa ttctggatca ggctgggttt aagacctgga atttaagcgg
     2521 cggttaccgg ctttatgaaa tggtaaagaa ggatgaggca gctatggcgg cagtagccgg
     2581 catgtgtacg gagtgcggaa aggaactgta ataaagtatt gacgcggaat gtgtgttgcg
     2641 gtaggcttgt aatgagacac attccgcatt tttatgtgaa ataatccgaa aggagaaggc
     2701 agaatatgaa gattacctat atccaccaca gcagttttct ggtggaaatg gagcagattg
     2761 cgttgctctt tgattatttt gagggagaga tcccagaaat ccgacaagat cagctgctga
     2821 ttgtgtttgc cagccatcgt catggagatc atttctcgcc tggaattttt gatctggcag
     2881 agaatcatcc caaaatctgg tttgtccttt cggacgatat ctggagaaaa agagttccgg
     2941 aggaattttt gtgtcggacc agttttttgg ggccgggaga ggaattttct tttcagaagg
     3001 agacaggaga aaaggtgcct gtaacggtca aagcatttaa atctacagat gagggtgtgg
     3061 cgtttttgat tgaggcagag ggaaaggtgc tttatcacgc aggagatctg aataactggg
     3121 tctgggaggg tgaaccggag gaatataact gccgaatgag cgaagcattt cacagtgaac
     3181 ttctgaaaat agctggaaat catattgatg tggcttttat gctgctggat ccccggcagg
     3241 gaaaggactt ttatctggga atggatgatt ttatgcatat ggtgggagct gatattgtgt
     3301 ttcccatgca tttctgggga gattttggcg tgacagagcg ttttaagact ctgccctgtg
     3361 cagctgacta tgcagaaaag attatggaaa ttcgttgtca gggggaaagc tttcagttat
     3421 agtataaatt gcggaaaaga aaggaagaaa cagttatatg atacttatca agggaagccg
     3481 ggtactggat ccggcgggaa agacagacag cagagcagat cttgtgattg aggatggaat
     3541 catcagagaa atctgccctc cggatacgct gggaggaaga acagaagcgg aatatgaaca
     3601 gataatcgat ggaacgggtc tggtgacggc tccgggtctg gtggatgttc atgtccattt
     3661 cagagatccg ggcctgacct ataaagagga tattcataca ggagcggctg cagctgccaa
     3721 aggaggcttt actactgtag tatgtatggc aaatacaaag ccgttagttg ataatgagga
     3781 aacgctgcgc tatgttcttg aagaggggaa aaagacagga atccatgtac tcagctgtgc
     3841 cgctgtgtca aagggattta agggcgaaga actgaccgat atggagggcc tgctggctgc
     3901 aggagccgcc ggctttacag atgacggaat ccccttaatg gacggcccct ttgtgcttaa
     3961 ggctatggag gaggccgcca gactggatac agtgatcagc
//
LOCUS       DBMDUH010000189         3971 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_8180_length_3971_cov_5.184755, whole genome shotgun sequence.
ACCESSION   DBMDUH010000189 DBMDUH010000000
VERSION     DBMDUH010000189.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3971)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3971)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3971
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8180_length_3971_cov_5.184755"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="ACZPCG_12145"
     CDS             complement(<1..315)
                     /locus_tag="ACZPCG_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HIR1285834.1"
                     /translation="MKIRHLLTGLAIMVSFMAASAFTALAAPAKGRFEEVTEHAITGW
                     AYDSDEPDEALNVNITIKNEETGKEVLNQSVKAGEFRQELLDDDKGNGNHGFTLNVDW
                     PSF"
     gene            549..620
                     /locus_tag="ACZPCG_12150"
     tRNA            549..620
                     /locus_tag="ACZPCG_12150"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:582..584,aa:Arg,seq:ccg)
     gene            759..1781
                     /locus_tag="ACZPCG_12155"
     CDS             759..1781
                     /locus_tag="ACZPCG_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285835.1"
                     /translation="MKRRVKWKSSYKGPGDNKSRYVKLGAAAAAVLAVLAAGSLMISH
                     FAGKDGTHQDGVIEILGEDVSHGTEEQTSEAAEADEPSEASLAEENGSEAETVTDFSA
                     YDLEKDAYPQVNEVVGAYFQAKIDQDPAALYQVFGKADDGNMDYYKKALKEEAVYVQD
                     YQDVTCYTRKGLTEDSFVVYVTYDVKFNRVDTLAPGIMWCYVAKDESGSYKIRENVIG
                     EEADYVAEQNRTEGVRLLNRQVKAKLKEAIESDTLLAGVYKGLSNGAIVTGDDSAQEG
                     SDSQVQILEGGISTDSPESQEAAEEAGSGEAEAEADTQSSGEETAPEQTVQESQSESE
                     ASVSIE"
     gene            1822..2859
                     /gene="queA"
                     /locus_tag="ACZPCG_12160"
     CDS             1822..2859
                     /gene="queA"
                     /locus_tag="ACZPCG_12160"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003501566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HIR1285836.1"
                     /translation="MDVKDFYFDLPQELIAQDPLEDRSSSRLLVLDKNTGAIEHRVFK
                     DIVNYLRPGDCLVVNDTKVIPARLFGVKKDTGAKIELLLLKRRENDIWETLVKPGKKA
                     KPGTVIDFGDGLLTGTVLETVDDGNRLIQFSYEGIFEEILDQLGQMPLPPYITHQLKD
                     KNRYQTVYAVHEGSAAAPTAGLHFTKELLKQIEDMGVKIAHVTLHVGLGTFRPVKVEN
                     VLDHHMHSEFYVVEESEAKKVNDTKAAGGRVICVGTTSCRTVESASTEDGILQAKTGW
                     TDIFIYPGYRFKILDALITNFHLPESTLVMLVSALAGREHILNAYEEAIRERYRFFSF
                     GDAMFIVNGQE"
     gene            3261..>3971
                     /locus_tag="ACZPCG_12165"
     CDS             3261..>3971
                     /locus_tag="ACZPCG_12165"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002573601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIR1285837.1"
                     /translation="MPVEERLNKWLSRMGVCSRREADRLIEEGKILVDGRPAVTGQKV
                     SEGQRVVCGGRIVGKAGEKEKTPRQVLLAVNKPRGIVCTTSDKDRAQNIVEFLAYPER
                     IYPIGRLDKDSQGLLLMTNQGDLVNKIMRCGNGHEKEYEVTIDRPVTEEFLKKMASGL
                     WLAELERKTLPCKVRKTGDKSFSIILTQGLNRQIRRMCEACGCYVRTLKRVRIMNIHL
                     GNLKEGSFREITGEEYRTL"
BASE COUNT         1123 a          779 c         1099 g          970 t
ORIGIN      
        1 gaaggagggc cagtccacat tcagagtaaa gccatgattt ccgttgcctt tatcgtcatc
       61 gagcagctcc tggcggaatt ctccggcctt tacggattga tttaagactt cttttcctgt
      121 ttcttcattt ttgatcgtaa tatttacatt cagcgcctca tctggctcat cgctgtcata
      181 ggcccagccg gtgatggcgt gctcagtcac ttcttcaaag cggccctttg caggagctgc
      241 cagagcagtg aaggcagagg cagccataaa ggaaaccatt attgccagtc cggtgagtaa
      301 atgtcgtatc ttcattataa aataacctcc atgcgttcat aattgcaatc aatgcttaac
      361 acaataataa taatatttaa cacttttgta atatatgagc aacaaatgcc attttagcat
      421 aattttttcg tatgtcaagg aaaaaaatgg taattcattg gaaaagagat aaaaaagatc
      481 ggaaaacaag attggaaata aaaaaagtag ttgcagtttt ttaaaaagta tagtaatata
      541 atagagatgc gtctgtagct cagtggatag agcagtggcc tccggagccg cgtgcgtagg
      601 ttcgattcct atcagacgca tttttgatac cttattctgc attacacaga gaaaccaggg
      661 ggcgtgtcct cctggttttt ctgttgcaat ttgaatccct ttttgctaga atagagcgga
      721 gatacggcct ttatggggcc tgaaacggag gtcagaggat gaagcgcagg gttaaatgga
      781 aatcaagcta taaagggccc ggagataata aaagcagata tgtaaagctg ggagctgctg
      841 cagctgcggt tttggcagtc ctggctgcag gttctcttat gatatcccat tttgcgggga
      901 aggacggcac acaccaggat ggcgtaattg agattctggg cgaggatgtg agtcacggca
      961 cagaggagca gacctctgag gcagcagagg cggatgagcc ttcagaagct tcattggcag
     1021 aagaaaacgg ctcagaagca gagacagtga cggatttttc tgcgtatgat ctggaaaagg
     1081 atgcctatcc gcaggtaaac gaagtagtag gagcctattt tcaggcaaag atcgatcagg
     1141 atccggctgc gctctatcag gtttttggaa aggccgatga cggaaacatg gactattata
     1201 aaaaggcctt gaaggaagaa gccgtatatg tgcaggatta tcaggatgtg acctgttata
     1261 ccagaaaggg gcttacagag gattcctttg tggtttatgt gacctatgat gtgaagttta
     1321 accgggttga taccctggct ccgggcatca tgtggtgcta cgtggcaaag gatgagagcg
     1381 gaagctataa aatccgggaa aatgtcattg gcgaggaagc cgattatgtg gcagagcaga
     1441 accggactga gggggtacgg ctcctcaatc ggcaggtaaa ggcaaaatta aaagaggcca
     1501 tagagtccga tacgctttta gcaggcgttt ataagggctt aagcaacggc gcgatcgtca
     1561 caggagatga cagcgcacag gaaggctctg acagccaggt acagattttg gagggaggaa
     1621 tttccacgga cagtccggaa tctcaggaag cagcggaaga agccggatca ggagaagctg
     1681 aagcagaggc agatacacag agttcaggtg aagaaacagc accggagcag acggttcagg
     1741 aaagccagag cgagtccgag gcttcagtca gtatagaata aaacacaggc gcatactgcg
     1801 ccgtttggag gaaaaacgat catggatgta aaggattttt attttgatct gcctcaggag
     1861 ctgatcgctc aggatccctt agaggacaga tcttcatcaa ggcttctggt gctggataag
     1921 aacacagggg cgattgaaca cagagtattt aaggatattg taaattatct ccgtcccgga
     1981 gactgtctgg tggtcaatga caccaaggtt attcctgccc gattgtttgg cgtaaagaag
     2041 gatacggggg caaaaataga acttcttctg ttaaaaagaa gggagaatga tatttgggaa
     2101 actctggtaa aaccggggaa gaaggcaaag ccgggaacgg tcattgactt tggagacggg
     2161 cttcttacag gaacggtttt ggagactgta gatgacggaa accgcctgat tcagttttcc
     2221 tatgagggca tttttgagga aattttagac cagctgggac agatgcccct tccgccctat
     2281 atcacccatc agttaaagga taaaaaccgc tatcagacgg tatatgcagt tcacgagggt
     2341 tctgcggcgg ctcccacagc aggactccat tttacaaagg agctgttaaa gcagattgag
     2401 gatatgggag ttaagattgc tcatgtgacc cttcatgtgg gccttggcac cttccgtccg
     2461 gttaaggtgg aaaatgtcct tgatcaccat atgcattctg agttttatgt ggtagaggaa
     2521 tcagaggcta aaaaggtgaa tgacactaaa gcagcgggag gaagagtgat ctgcgtgggc
     2581 actaccagct gccgtaccgt ggaatctgct tctaccgagg acggcatttt gcaggcaaaa
     2641 acaggctgga ccgatatttt catttatccg ggctaccgtt ttaaaatcct ggatgccctg
     2701 atcaccaatt tccatctgcc ggaatctact ttggtaatgc tggtcagcgc tctggccgga
     2761 agagagcata tcctgaatgc ttatgaggag gccatccggg agcggtatcg tttcttctcc
     2821 tttggggatg cgatgtttat tgtgaatggt caggagtaag gaaaaaacgg ccgggatgaa
     2881 agaaagttaa gcgaaaaagc gtcaggatac gtttgaccgg acgctttttc gcttattgca
     2941 tttgcgggtt ttacgctata cagatcccat gtacagtacg gttttgttcc ccacatgaac
     3001 cgctccgttg tgaaaatgcc ccggctgaat ccttgaaagc tgctgcaata cctgtggaaa
     3061 atctgttcgc ctgatccatt tacaagcatt tttcgttcaa aaccggctaa ttggatctgc
     3121 gcctgttttc tcatatcctt atttggctct gccgcagacg gcaaatacag aagcaaaggc
     3181 cttttgttgc ctgaatgtgg aattgatgat atactgatgg aacatatgat agttgtgaaa
     3241 gttttgatga aaagagaggg atgcctgtgg aagaacgttt aaataaatgg ctgagccgca
     3301 tgggcgtctg ttccagacgg gaagcagatc gtctgattga ggaaggaaag atcctggtgg
     3361 acggaagacc ggctgtaaca ggccagaagg tgtctgaggg ccagcgtgtg gtctgcggcg
     3421 gccggattgt gggaaaagct ggggaaaagg aaaaaactcc cagacaggtg cttttagcgg
     3481 taaataagcc cagagggatt gtatgcacta cctctgataa agacagggca caaaacattg
     3541 tggaatttct ggcatatccg gagcggattt atcccatcgg ccgtctggat aaggactctc
     3601 agggcctttt actgatgacc aatcaggggg atctggtaaa taaaatcatg cgctgcggca
     3661 atgggcatga aaaggagtat gaggtaacca tagaccgccc ggttacggag gagtttctga
     3721 aaaaaatggc gtccggcctg tggctggctg agctggaacg aaagacactg ccctgtaagg
     3781 taagaaaaac aggagataaa agtttttcta tcatactgac tcagggctta aaccggcaga
     3841 tccggagaat gtgtgaggcc tgcggctgct atgtgcgcac tctgaaacgg gtgcggatta
     3901 tgaatattca tctggggaac ttaaaggaag gctctttcag ggaaataaca ggagaggaat
     3961 acaggacgct t
//
LOCUS       DBMDUH010000190         3966 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_8192_length_3966_cov_5.120828, whole genome shotgun sequence.
ACCESSION   DBMDUH010000190 DBMDUH010000000
VERSION     DBMDUH010000190.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8192_length_3966_cov_5.120828"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /locus_tag="ACZPCG_12170"
     CDS             complement(<1..1178)
                     /locus_tag="ACZPCG_12170"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HIR1285838.1"
                     /translation="MIPNTNYNNLKESYLFYHIAQKTKLYLEEHPEAHLYRMGIGDVS
                     LPLCGEVIKALHKAVDDQGEKSRFHGYMPECGDPELRKIIAEYYKKRGVGLSQEEVFI
                     SSGASDELGDILDLFGRDKTVMIMEPAYPAYVDANIITGNKIIHIPSGEKEGFLPLPD
                     ESVTADVIYICSPNNPTGAVFNYDKLKQWVDYANRTDAVLLFDAAYEAFIEDEDVPHS
                     IFEIEGAKNCAIEICSLSKTAGFTGTRLGYTVIPGALKRNGMSLNEMWIRNRTTKTNG
                     VSYIIQKGGAAVFTEEGQRQIRERIQVYKNNAKVLMDALDQLGIWYCGGKNAPYIWMK
                     CPNGMGSWEFFDYLLHEIQVVGTPGEGFGACGEGYFRFSTFGSPEDTKEAAGRLVKLL
                     A"
     gene            complement(1194..2618)
                     /gene="purF"
                     /locus_tag="ACZPCG_12175"
     CDS             complement(1194..2618)
                     /gene="purF"
                     /locus_tag="ACZPCG_12175"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004071649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HIR1285839.1"
                     /translation="MGIHEECGVFGVMNKKKEPVAELVYYGLYALQHRGQESCGIVVN
                     DDGVFSSYKDLGLVSEVFSKDTLSHLPEGTIAVGHVRYGTTGGNTRSNCQPIEVNHQK
                     GKMALAHNGNLSNALELRDRLELSGAIFHTTSDTETIAYIITRERLKTPSIEEAVSNA
                     MESLEGAYSLVLMSSTKMIAARDPYGFRPLCYGKMPDGAYVVASESCALSAVGAEFIR
                     DVLPGEILVFHPDGVESRREHCGKQKKKTCIFEYIYFARPDSVIDGVSVHESRIRAGE
                     LLAETYPADADIVIGVPDSGLDAALGFAKKSGIPYGIGLIKNKYIGRTFISPGQSERL
                     DQVKIKLSPVKSVIEGKNVVLIDDSIVRGTTSRRIVKLLREAGAKEIHMRISAPPFLH
                     PCYYGTDIDSEENLIASHHSTAEIAKLIGVDSLGYLQVDQLNRLIDSNDYCAACFHGQ
                     YPTKIPTDLRKDRFERKLSERNNQ"
     gene            complement(2697..2804)
                     /locus_tag="ACZPCG_12180"
                     /pseudo
     CDS             complement(2697..2804)
                     /locus_tag="ACZPCG_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003523395.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine--fructose-6-phosphate
                     aminotransferase"
     gene            complement(2815..>3966)
                     /locus_tag="ACZPCG_12185"
     CDS             complement(2815..>3966)
                     /locus_tag="ACZPCG_12185"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HIR1285840.1"
                     /translation="PFLEAVRQISLEVGQENSLFIHVTLVPYLSGSEEHKSKPTQHSV
                     KELRGMGINPGIIVLRSDRPLEESVFRKISLFCNVREDCVIENITLPNLYEAPLMLEK
                     SGFSDVVCRELHIEAPEPDLTDWTAMVERIHNRSEEVHIGLVGKYVKLHDAYLSVAEA
                     MNHAGYEENAFVKIHWIDSEGITRETVNDVLHGLDGIIVPGGFGSRGIEGMILSAKYA
                     RENRIPYFGICLGMQIAVIEFARHVLGLTDAHSGEFDEQCAHRVIDFMPGQSGEIDKG
                     GTLRLGSYPCCIKAGTKMEECYGSEQIHERHRHRYEFNNEYRGELEKAGLVISGTSPD
                     GRLVETIELPEHPFFIGVQFHPEFKSRPNQPHPLFKGFISAALKQTEEK"
BASE COUNT          952 a         1095 c          797 g         1122 t
ORIGIN      
        1 gcaagcagct tcacaagccg cccggctgcc tctttcgtat cctccggcga gccaaaggta
       61 gaaaaacgga aatatccctc gccgcaggct ccaaagcctt ctcccggagt tcccaccacc
      121 tggatttcat gaagcagata atcgaaaaat tcccagcttc ccattccatt cggacacttc
      181 atccagatat aaggagcatt ttttccgccg caataccaga ttcccagctg atcaagggca
      241 tccatcaata ccttcgcatt atttttataa acctgtattc tttcacggat ctgcctctgt
      301 ccttcttccg taaaaacagc ggctcctccc ttttggataa tgtaagatac cccgttggtt
      361 ttcgttgtac ggttgcgtat ccacatctca tttaagctca tcccgtttcg tttcagcgct
      421 ccgggaatca ctgtatagcc caatctggtg ccagtgaagc ccgctgtttt agacagggaa
      481 catatttcga tcgcacagtt ttttgccccc tctatttcaa aaatactgtg gggaacatcc
      541 tcatcttcaa taaacgcctc atatgcggcg tcaaacaaaa ggacagcgtc cgtgcggttg
      601 gcataatcta cccactgctt taatttgtca taattgaaaa cggctcctgt agggttattg
      661 ggggagcaga tgtaaatgac atccgctgta acgctttcat ccggcagcgg aagaaatccc
      721 tccttctctc ccgatgggat gtgaatgatt ttattccctg tgattatatt ggcgtctaca
      781 taagccggat aggccggttc catgatcata accgttttat ccctgccaaa cagatcgaga
      841 atatctccca gctcgtcact tgccccgctg gaaataaata cttcctcctg tgaaagccct
      901 acgcctcttt ttttataata ttcggcaatt atctttctca gttcagggtc tccgcactcc
      961 ggcatatagc cgtgaaaacg gctcttttcc ccctgatcgt cgactgcttt gtgaagcgct
     1021 ttgatcacct cgccgcagag aggaagggat acgtctccga ttcccatacg atacagatgc
     1081 gcctccggat gctcttccag ataaagcttt gttttctggg caatatgata aaacagatag
     1141 gattccttca aattattgta attggtattc ggtatcatag attttattcc tttctactga
     1201 ttgtttctct cagacagctt tctttcaaat cggtctttcc gaagatccgt tggtattttc
     1261 gtaggatatt gtccatgaaa acaggctgcg caatagtcat tgctgtcaat cagcctgttc
     1321 agctgatcca cctgcagata tcccagagaa tcgactccga tcaacttcgc gatttccgct
     1381 gtactgtgat ggctggcaat cagattttct tctgaatcaa tatctgttcc atagtagcag
     1441 ggatgcagga agggaggcgc ggaaatcctc atatggatct cctttgctcc cgcttcgcgc
     1501 aaaagcttta cgatacgcct gctggtcgtt cctctgacaa tggaatcatc gatcagaacc
     1561 acatttttcc cctcaatcac actttttaca ggagaaagct tgatttttac ctgatccaga
     1621 cgttcgctct gccccggaga tataaacgtc cgtccaatat atttgttttt aatcagtcct
     1681 atgccatagg gaatgccgga ttttttcgca aagcccaggg cagcgtccag accgctgtcc
     1741 ggaactccga tcacaatatc cgcgtctgcc ggataggtct ccgccagcag ttccccagcc
     1801 cttatccgcg attcatggac agatactccg tctatcacag aatccggtct ggcaaaatag
     1861 atatactcaa aaatacaggt ttttttcttc tgttttccgc aatgctctct tctggattcc
     1921 acaccatccg gatgaaaaac caggatttct cccggcagta cgtctctgat aaactctgcc
     1981 cccactgctg acaatgcgca gctttctgaa gccacaacat aagcgccgtc cggcatcttt
     2041 ccatagcaca ggggtctgaa gccatagggg tcgcgggcgg cgatcatctt cgtggaagac
     2101 ataagaacca gggaataggc cccttccagc gactccatcg cattgcttac agcttcttca
     2161 atactgggtg tcttcagccg ttctctggta ataatatatg cgatggtttc cgtatcgctg
     2221 gtggtatgaa aaatagctcc tgacaactcc agcctgtcgc gaagctccag agcattgctc
     2281 agatttccat tgtgagccag agccattttc cccttctggt gattgacttc aatgggctgg
     2341 cagttgcttc tcgtgtttcc tcctgtggtt ccatatctta catgtcccac agctatggtt
     2401 ccctccggca gatgggatag tgtatcttta gaaaatactt cgcttacaag cccaaggtct
     2461 ttataggaag aaaatactcc gtcgtcattg acaacgatcc cgcagctttc ctgtcctctg
     2521 tgctgcaatg catagagtcc gtaataaacc agctctgcca caggctcctt ttttttgttc
     2581 attactccaa atacgccgca ttcctcatga attcccatcc cgttatttcc tcctactgat
     2641 ctttccttac tctaccgtaa ccgattttgc cagatttctc ggtttatcaa tatcacaacc
     2701 gcaacgcctg ccgaatcata tcctctgtat tccagccgtt ttaaaccgtc caacagtacg
     2761 gaaaccgcct ctgtatttcc tgcgaatccg ataattccac acatatttct actcctattt
     2821 ttcttctgtc tgctttaatg ccgcagaaat aaagccttta aacaggggat gaggctgatt
     2881 ggggcggctc ttaaattcgg gatgaaactg tacgcctata aaaaatggat gctccggcag
     2941 ctctatggtt tcaaccagcc tgccgtctgg agaagttccg cttatgacca gtccagcttt
     3001 ctccagctcc ccgcgatatt cattattaaa ctcataacgg tggcggtgac gctcatggat
     3061 ctgttctgat ccgtagcact cttccatttt cgtcccggcc ttgatgcagc agggatagct
     3121 tcccagacgc agcgtgcctc ccttatcaat ctccccgctc tgtcccggca taaaatcaat
     3181 taccctgtgg gcgcactgct cgtcaaattc tcctgaatgg gcatccgtaa gccccagaac
     3241 atgccgtgca aattcgataa cagcaatctg cattccaagg cagatgccaa aataaggtat
     3301 cctgttttca cgggcatact ttgcagacag gatcatgcct tcaattcccc ggcttccaaa
     3361 accgccggga acgataattc catccagtcc atgcagaaca tcatttacag tttccctggt
     3421 aatcccttcg gaatcgatcc agtggatttt tacaaatgca ttctcttcat atccggcatg
     3481 attcattgct tctgctacag acagataggc gtcgtgaagc tttacatact ttccaaccag
     3541 gccaatgtgt acttcttcag aacgattgtg gatccgttct accatcgccg tccagtctgt
     3601 cagatccggc tctggcgcct caatgtgaag ctccctgcac accacatcgg aaaaaccgga
     3661 tttttccagc atcagcggcg cttcatagag atttggcagc gttatgtttt caatcacaca
     3721 gtcttccctg acattgcaga acagggaaat cttccgaaaa acagattcct ctaatggcct
     3781 gtcagaacga aggacaataa taccgggatt gatccccatt ccgcgaagct ccttaactga
     3841 gtgctgtgtc ggctttgact tatgctcctc tgaaccgctc agataaggaa caagcgtcac
     3901 atggataaac aggctgtttt cctgtccaac ctccagcgaa atctggcgta ccgcctctaa
     3961 aaacgg
//
LOCUS       DBMDUH010000191         3943 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_8244_length_3943_cov_7.801406, whole genome shotgun sequence.
ACCESSION   DBMDUH010000191 DBMDUH010000000
VERSION     DBMDUH010000191.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3943)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3943)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3943
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8244_length_3943_cov_7.801406"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            25..909
                     /gene="lsrF"
                     /locus_tag="ACZPCG_12190"
     CDS             25..909
                     /gene="lsrF"
                     /locus_tag="ACZPCG_12190"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HIR1285841.1"
                     /translation="MADLEGVKVAKNYHLDAPFANQEGFYVKGANALDWGMKKHMSNI
                     FDAKSGNTVMFAFDHGYFMGSTAGLERLDLIIPKLIPNIDVLMGTRGALRSCVDPALI
                     GGKGVALRATSGSSMLNDDLSHEVLAVDAAEACRMNADCLAIQTFVGADGQLSSLDNL
                     SKAVNLGQQYSIPVMGVVAVGKQMERTDRFFKLATRMVAELGANIVKSYYCDNFEEVV
                     AACPVPIVVAGGKKLPEDEALTLAYRSIQGGAKGLDMGRNIFQSNHPVEMSAAIRKIV
                     HEGFTDKEAYEFYQDAIH"
     gene            complement(995..2341)
                     /locus_tag="ACZPCG_12195"
     CDS             complement(995..2341)
                     /locus_tag="ACZPCG_12195"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HIR1285842.1"
                     /translation="MMADIWDFFENMDELVYVADMDTYDMVYMNRRAMAQYALTSPEQ
                     YQNRKCYELLQSCSSPCAMCTNSKLCPGEFFEWSRYNPIVKRAYILKDTMVIRDGRRL
                     RIEITIDMNVHQREKLTIEDQTDTEALINEGLRFALAAETPIRSIDILLQYMGQSFSG
                     ERAYIFERNSQNSFDNTFEWCSDQVLPQKNILINIPEQDLNSWVQAFNRNENVLITDI
                     EAIRTADPDVYSLLKPQNIHSLVVSPIMDGRRLIGFYGIDNPPLELMSHISFTLQLFG
                     HFISSILKRRDLVERLENLSFYDQLTGAKNRHAMNEFLSVLPIKTSLGILYADVMGLK
                     QINDTQGHQAGDQTLIRCCRCLMEHFPKNTVFRVGGDEFLILCENWSQMEFEHAVNCL
                     KNDMIEGVSFMAVGSVWLPCCDENVEKYISDADSRMYENKRAYYQSKGHDRRKRME"
     gene            2578..3516
                     /gene="cysK"
                     /locus_tag="ACZPCG_12200"
     CDS             2578..3516
                     /gene="cysK"
                     /locus_tag="ACZPCG_12200"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HIR1285843.1"
                     /translation="MAGILNGITELVGNTPLMEAVNLEKKEGLKARLLLKLEYLNPAG
                     SVKDRVAKAMIEDAEAKGLLKEGSVIIEPTSGNTGIGLAAIAASKGYQVILTMPDTMS
                     EERRNILKAYGAKLVLTPGAKGMSGAIAKAEELAKEIPQSMIAGQFVNPVNPKAHRES
                     TGPEIWRDTEGQVDIFVASVGTGGTITGTGGYLKEKNPHIQVIAVEPAASPVLSGGTA
                     GAHKIQGIGAGFVPEVLDTEVYDEIIAVDNEIPFETARMLAKTEGIQTGISSGAALYA
                     ALQAAKRPENEGKTIVVLLPDSGDRYYSTPLFQAGE"
     gene            3603..>3943
                     /locus_tag="ACZPCG_12205"
     CDS             3603..>3943
                     /locus_tag="ACZPCG_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HIR1285844.1"
                     /translation="MSFLLFLSDAMVPLMIFYIVGFGILSGRPVLDDFIRGVKDGVKT
                     VGGIFPTLIGLMVSVGILRSSGLLDAFGELLKAPASFLGLPHAVIPTALVRLVSNSAA
                     TGLVLDLFKEYG"
BASE COUNT         1050 a          838 c         1005 g         1050 t
ORIGIN      
        1 gaacaaagac aggagagtaa tattatggca gatcttgaag gtgtaaaagt tgcaaaaaat
       61 tatcatctgg acgctccatt tgccaaccag gaaggatttt atgtaaaagg tgcaaatgcg
      121 ctggactggg gcatgaaaaa acatatgtcc aatatttttg atgcgaagag cggcaatacg
      181 gtaatgttcg catttgacca tggctatttt atgggttcca cagcaggcct ggagagactg
      241 gatcttatca ttccaaagct cattcctaac attgatgtgc tgatgggtac cagaggggcg
      301 ctcagaagct gcgtagatcc ggcgctgatc ggcggaaagg gtgttgcact cagagctacc
      361 tccggttcct ctatgctgaa tgatgacctt tctcatgagg tactggctgt agatgctgca
      421 gaggcctgca gaatgaacgc agactgtctg gcaatccaga ccttcgtagg cgcagacgga
      481 cagctgtcca gtctggacaa cctttccaaa gcggtaaatc tgggccagca gtattcaatc
      541 cctgtgatgg gcgttgttgc agtaggcaag cagatggagc gtacagatcg tttctttaag
      601 ctggctaccc gtatggtggc tgaactgggc gccaatattg taaaatccta ttactgcgat
      661 aactttgaag aagttgtagc tgcctgccct gttccgatcg tggttgcagg aggaaagaag
      721 cttcctgagg atgaagctct gacactggct tacagatcca ttcagggcgg agcaaaaggt
      781 cttgacatgg gaagaaatat cttccagtcc aatcatccag tagagatgag tgcagcgatt
      841 cgtaagatcg ttcatgaagg ctttaccgat aaagaggcat atgagttcta ccaggatgcg
      901 atccactaag cgcgcagcct gacgtgttta ctgttatatt ataaaaaagc cgctgcggag
      961 attatttttc cggcagcggc tcatttttta tgccttactc catgcgcttt ctgcggtcgt
     1021 gacccttgga ctggtagtag gctcttttat tttcatacat ccggctgtcc gcatctgaaa
     1081 tgtatttttc tacattttca tcacagcatg gaagccacac ggagccgaca gccataaagg
     1141 acacgccctc tatcatatca tttttcaggc agtttactgc gtgttcaaat tccatctgag
     1201 accagttctc acacaggatc agaaattcat cgcctccaac gcgaaagacg gtatttttag
     1261 gaaaatgctc catcagacag cggcagcatc ggatcagcgt ctgatcgccg gcttgatgcc
     1321 cctgggtgtc attgatctgt tttaggccca ttacatcggc gtacagaata cccaggcttg
     1381 tcttgattgg gaggacgctt aaaaactcgt tcatggcatg acggtttttg gcgccggtga
     1441 gctgatcata aaagctcagg ttttcaagcc gctccaccag atcccgccgc tttagaatag
     1501 aagaaataaa atggccgaag agctgcagtg taaaggaaat atggctcatc agctccagcg
     1561 gtggattgtc aattccataa aaaccaatca gccggcgtcc atccattatg ggactgacaa
     1621 ccagagagtg tatgttttgc ggctttagca gagaataaac atctggatcc gctgtacgga
     1681 ttgcttcaat atctgtaatc aggacatttt catttcgatt aaaggcctgt acccaactgt
     1741 ttaagtcctg ttcaggaata tttatgagga tatttttctg aggcagcacc tggtcagaac
     1801 accattcaaa ggtattgtca aaggaattct ggctgttccg ctcaaagatg taggcccgtt
     1861 ctccgctgaa gctctgtccc atatattgaa gcaggatatc aatagaacgg atcggtgttt
     1921 ctgcggctaa cgcaaagcga agcccctcgt tgatcagcgc ttctgtatcg gtttgatcct
     1981 caattgtcag cttttcccgc tggtggacgt tcatatcgat cgtaatttca atccgtaatc
     2041 ttctgccgtc ccggattacc atggtatctt tcaggatata agctcttttt acaatgggat
     2101 tataccggga ccattcaaaa aattctccgg ggcagagctt gctgttggta cacatggcac
     2161 agggagaaga acagctctgg agcagttcgt agcatttgcg gttctgatac tgttcaggtg
     2221 atgtaagtgc gtattgtgcc attgcccgcc ggttcatata taccatatcg taggtatcca
     2281 tatcggctac ataaacaagt tcatccatat tttcaaaaaa atcccatata tctgccatca
     2341 tacaactccc ctccattatg attttttgta catggaagaa tttttctata ttatatcgga
     2401 tttttcggta aaatggcatc tttttttgat ttttgttaaa aacattggag aataaatatt
     2461 ccgcttcaaa cggatcggtt cacgtatgcc ttgacaaaag gaatggtggg ttctataatc
     2521 caaccaaatc agactggcac caaacgatgg aacgccgaaa aggaaaggat gaggtttatg
     2581 gcaggtatat taaacggaat cacggagttg gtgggaaata cgcctttgat ggaggccgta
     2641 aatctggaaa agaaggaagg attaaaagca aggctccttc tgaagctgga gtatttaaat
     2701 ccagcaggaa gtgtaaagga tcgtgttgca aaggccatga tagaggatgc agaggcaaag
     2761 ggattattaa aagaaggttc tgttatcatt gaacctacct caggaaatac agggattggt
     2821 ctggcagcga ttgcggcctc gaagggatat caggtgatcc taaccatgcc cgataccatg
     2881 agtgaggaac gcagaaatat attaaaggca tatggggcaa agcttgttct tacgccaggc
     2941 gccaagggaa tgagcggagc cattgcaaag gcagaggagc tggcaaagga aattccccaa
     3001 agtatgattg caggccagtt tgtaaaccct gtaaatccca aagcccacag agagtctacg
     3061 gggcctgaga tctggagaga cacagaggga caggtggata tttttgtggc ctctgtagga
     3121 actgggggca cgattacagg aaccggagga tatttaaagg aaaagaatcc gcacattcag
     3181 gtaatagcag tggaaccggc tgcttcacca gtgctgtcag gcggtactgc cggagcgcat
     3241 aagattcagg ggatcggagc gggatttgtg ccagaggtgc tggatacaga ggtatatgat
     3301 gagatcattg ccgtggataa tgaaattccc tttgaaacag cccgtatgct ggctaaaaca
     3361 gaggggatcc agacgggtat ttcttccggc gcagcgctgt atgcagcctt acaggctgcc
     3421 aagcggcctg aaaatgaagg aaaaaccatt gttgtactgc tgccggacag cggagaccgg
     3481 tattattcta cgccgctttt tcaggcaggt gaataatacc aggcattggg agacagccgt
     3541 catataacat aatcaccgcc tcacaggaat aagctgtata aaaatcctgt ggggcggttt
     3601 ttatgtcttt tctgcttttt ctttcagatg ctatggtgcc tcttatgatc ttctatatcg
     3661 taggctttgg tattttatcc ggacgtcctg tgctggatga ttttatccgg ggagtaaagg
     3721 atggggtgaa aacagtaggg gggatcttcc ctacactgat cggcctgatg gtatcggtag
     3781 gtatattgcg ttcctccggc cttttggatg cgtttggaga actgttaaag gcgccggctt
     3841 cttttctggg actgcctcat gccgttatcc ccacagcgct ggtacgtctg gtgtcaaatt
     3901 ctgcggcaac aggcctggtt ttggatcttt ttaaggaata cgg
//
LOCUS       DBMDUH010000192         3910 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_8309_length_3910_cov_5.532948, whole genome shotgun sequence.
ACCESSION   DBMDUH010000192 DBMDUH010000000
VERSION     DBMDUH010000192.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3910)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8309_length_3910_cov_5.532948"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            258..341
                     /locus_tag="ACZPCG_12210"
     tRNA            258..341
                     /locus_tag="ACZPCG_12210"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:292..294,aa:Leu,seq:cag)
     gene            536..1273
                     /locus_tag="ACZPCG_12215"
     CDS             536..1273
                     /locus_tag="ACZPCG_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1285845.1"
                     /translation="MLRLENLSFEVNDDKGEKGIIKDISLTVDDGKFVVITGPNGGGK
                     STTAKLIMGIEHPTGGKIWFDGEDITEKSITDRAKMGISFAFQQPVRFKGVQVIDLLR
                     LASGKKLTVSEACQYLSEVGLCAKDYINREVNGSLSGGELKRIEIATILARGAKLSVF
                     DEPEAGIDLWSFQNLIQVFERMRQNTKGSILIISHQERILNIADEIVVIADGKIVNQG
                     TRDEIMPKIMGTASAVDACSGFLDSVQ"
     gene            1288..2205
                     /locus_tag="ACZPCG_12220"
     CDS             1288..2205
                     /locus_tag="ACZPCG_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufB/SufD family protein"
                     /protein_id="HIR1285846.1"
                     /translation="MDQIEERLLEEVADLHGVPEGAYNIRANGQMAGRNTTANIDITT
                     KTDKPGIDIRIKAGTKHESVHIPVVLSASGLKEVVYNDFYIGDDADVVIVAGCGIHNC
                     GVQDSEHDGIHRFFVGKNAKVKYVEKHYGEGDGEGKRLLNPVSEVYMEENSLMEMEMV
                     QIKGVDSTNRTNKAVLAAGAKLLVRERLLTHGDQVAESTYDVELNGDGSSADVVSRSV
                     AKDHSKQIFKSNLIGNAKCSGHTECDAIIMDDAKIVAVPGLVADNIDAALIHEAAIGK
                     IAGEQIVKLMTLGLTEAEAEAQIVNGFLK"
     gene            complement(2229..3209)
                     /locus_tag="ACZPCG_12225"
     CDS             complement(2229..3209)
                     /locus_tag="ACZPCG_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018595103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIR1285847.1"
                     /translation="MNERLLRELSHITEEEQDILDGKTGIDRTLYSEGSDFVITSEKM
                     LKEGRLIQFRPHTRFVYFPRHTHNYVEMIYMCSGSTTHIINGTELILQRGELLILNQH
                     ASQEILPAGKEDVAVNFIILPEFFDQTLAMLGSEQSMLRDFVVGCLQTADSPLSYLHF
                     KVSDILPVQNLLENLLWTLSHELQNKRLMNQITMGLLFLQLLNYTDRIQAGRTSFEQE
                     ILMQVYQYVEEHYKDGELTSLAARLHCDLTWLSRAIRRLSGCTYTELVQKKRLNQACF
                     LLRSTALSVLDISMAVGYENFSYFHRIFKQTFGQTPRQYRMALKKEPEEG"
     gene            3381..>3910
                     /locus_tag="ACZPCG_12230"
     CDS             3381..>3910
                     /locus_tag="ACZPCG_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004841518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HIR1285848.1"
                     /translation="MERYYLAIDIGASSGRHMLGHIENGKMILEEIWRFENGMKDREG
                     SLCWDLDALFEEIKTGMKRCKELGKIPVSMGIDTWAVDYVLLDEKDQVLGKTYGYRDG
                     RTQGVDEDVYKIISEEELYSRTGIQKQSFNTIYQLMAVKKNNPEHLKAAKNFLMLPDY
                     FHFLLTGQKCSEYTNAT"
BASE COUNT         1132 a          793 c         1062 g          923 t
ORIGIN      
        1 catggcatac agtttaaagg caatggagat taccagagag agggtctatg agctgatgga
       61 gctgttggat tgatggcttg caatgttaat tacggagcat ggaagataat aaaggctaaa
      121 aagcggcaaa aaatctgtta aaaaatggcg caaaaaagtt aaaaaagtcg ttgacaaatc
      181 cgctgctatc cgatataata gtcttcgttg ctgagacagc cagtaagaat tactgctaaa
      241 cagcaaaaac agaaaatgca gaagtggcgg aattggcaga cgcgcacggt tcaggtccgt
      301 gtggtagtaa taccttgagg gttcgactcc cttcttctgc atgatatatg gcaggaagtc
      361 aggaaactgg cttcctgttt tgctatgtga caaatgcagc agacattttt tgccctaaga
      421 ggtaaattat aatagtaatt tgtccataaa tagtatatac tgtaagttgt gtggccattt
      481 gccgcagatg cggctggcct taaaacggga cgaaaagaga ggtaggagtg tcattatgct
      541 tagattggaa aatctctcct ttgaagtaaa tgacgacaaa ggagaaaagg gcattatcaa
      601 ggatataagc ctgacagtag atgatgggaa atttgtggtg atcacaggac ccaacggcgg
      661 cggtaaatcc accacggcta aactgatcat gggaatcgag catcccacag ggggaaagat
      721 ctggtttgac ggggaggaca ttacagaaaa gagcattaca gaccgtgcaa agatgggtat
      781 cagttttgca ttccagcagc cggttcgctt taagggagtt caggtgattg atcttctacg
      841 tctggcttcc ggcaagaagc ttaccgtgtc cgaggcctgt cagtatttat ccgaggtagg
      901 actctgtgcc aaggattata ttaaccgtga ggtcaatggc agcctgtcag gaggcgaatt
      961 aaagcgcatt gagattgcca ccattcttgc aagaggggca aagctgtccg tatttgacga
     1021 gcctgaggcg ggaatcgacc tttggagctt ccagaacctg attcaggtat ttgagcgcat
     1081 gcgccagaat acaaagggtt ccatcctgat catttcccat caggagcgta ttttaaatat
     1141 tgccgatgag atcgttgtga tcgcagacgg aaaaatcgta aatcagggta caagggatga
     1201 gatcatgcca aagatcatgg gaacggcttc tgcagtggat gcctgctccg gtttccttga
     1261 ttccgtgcag taagggagga catgaggatg gatcagatcg aagagagatt attagaggaa
     1321 gtggctgacc ttcacggagt gccggaggga gcctacaata tccgtgccaa cggccagatg
     1381 gcaggaagaa atacaaccgc caatattgac attaccacaa agacggacaa gccgggaatt
     1441 gacatccgca ttaaggcggg tacaaagcat gagagcgtgc atattccggt ggtattaagc
     1501 gccagcggct taaaggaagt ggtttacaat gacttctata tcggggatga tgctgatgtg
     1561 gtgatcgtgg caggctgcgg catccacaac tgcggagtcc aggattcgga gcatgacggt
     1621 atacaccgct tctttgtagg taaaaatgct aaggttaaat acgtagaaaa gcactatggc
     1681 gagggagacg gggaaggaaa acgtttgtta aatcccgttt ctgaagtata tatggaagaa
     1741 aacagcctga tggaaatgga aatggttcag atcaagggcg tagattccac caaccgcact
     1801 aacaaggccg tactggcagc aggtgcaaag cttctggttc gcgagcgcct tttaacccat
     1861 ggagatcagg tggcagaaag tacctatgat gtggaattaa acggagacgg ctccagcgca
     1921 gacgtggttt cacgttctgt tgcaaaggat cactcaaaac agatattcaa gtccaatctg
     1981 atcggaaatg caaagtgcag cggccataca gagtgtgacg ccattatcat ggacgatgcc
     2041 aaaatcgtag cagttcccgg tctggtggct gataatattg atgcggccct gatccatgag
     2101 gctgccatcg gaaagatcgc aggcgagcag atcgtaaagc ttatgactct gggactgaca
     2161 gaggcagagg cagaggccca gatcgtaaat ggcttcctga aataaagaga aagagccggg
     2221 gaagggattt aaccctcctc cggttctttt tttaacgcca tccggtattg tctgggagtc
     2281 tggccaaagg tctgcttaaa aatccggtgg aaatagctga aattttcata ccccacagcc
     2341 atgctgatat ccagcacgga cagagcggtg gaacgcagga gaaagcaggc ctggttcagc
     2401 cgcttttttt gaaccagctc tgtgtaggtg cagccggaga gacggcggat ggcccgggac
     2461 agccaggtta agtcgcagtg aagccgtgcg gcaagagaag tcagctctcc gtccttatag
     2521 tgctcctcca catactggta gacctgcatg agaatttcct gctcaaatga ggtgcgtccg
     2581 gcctgaatac ggtcagtgta atttaaaagc tgcagaaaga gaagccccat ggtgatctgg
     2641 ttcataagcc gtttattctg aagctcatgg gaaagggtcc agagcagatt ttccagcaga
     2701 ttctggacgg ggaggatgtc ggaaacctta aaatgcagat aggacagggg agagtctgca
     2761 gtctggagac agcctaccac aaaatccctc agcatactct gttcggagcc aagcattgca
     2821 agggtttgat caaaaaattc cggcagaatg atgaaattga cggccacatc ctctttcccg
     2881 gcaggaagga tttcctgaga ggcatgctga tttaaaatca gaagctcccc tctttgaagg
     2941 ataagttctg tgccatttat gatatgagtg gtagaaccgg aacacatata gatcatctcc
     3001 acgtagttgt gagtgtgtct gggaaaatag acaaagcggg tgtgggggcg gaactggatc
     3061 agccgcccct ctttgagcat cttttcgctg gtgatcacaa aatcagagcc ttctgaataa
     3121 agggttcggt ctatgcctgt ttttccatca aggatatcct gttcttcttc tgtaatatgg
     3181 ctcagttccc tcaaaagccg ttcattcata aatactcccc ttatctgtct gcagaaggca
     3241 tttttgctgt tcacagtata ccacaactct gcaaataagg acagtttttt agccaaaaac
     3301 cgttcttttt ttgcccgctg ccttcaggta caataaagcc atgacaaaac gtgtgatgaa
     3361 acggtaaaag gaggagggct atggaacgct attatctggc catagacatc ggagcttcca
     3421 gtggccgtca tatgcttggg catatagaga acgggaaaat gatcctggag gaaatctggc
     3481 gctttgaaaa cggcatgaag gaccgggaag gaagcctgtg ctgggatctg gatgcgctgt
     3541 ttgaggaaat caagacagga atgaagcgat gtaaggaact gggaaagatc cctgtatcta
     3601 tgggaattga cacctgggcc gtggattatg tgcttttgga tgaaaaggat caggtactgg
     3661 gaaaaaccta tggataccgt gacggccgca ctcagggagt ggatgaggat gtatataaga
     3721 tcatttcgga agaagagctt tacagccgca ccggaatcca gaagcagagc tttaatacca
     3781 tttatcagct gatggcagta aagaaaaata atccggagca cctgaaggcg gcaaaaaact
     3841 ttttaatgct gccggactat tttcattttc tgctcacagg acagaaatgt tcggagtaca
     3901 ccaatgcaac
//
LOCUS       DBMDUH010000193         3898 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_8351_length_3898_cov_7.582302, whole genome shotgun sequence.
ACCESSION   DBMDUH010000193 DBMDUH010000000
VERSION     DBMDUH010000193.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3898)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8351_length_3898_cov_7.582302"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1354)
                     /gene="brnQ"
                     /locus_tag="ACZPCG_12235"
     CDS             complement(<1..1354)
                     /gene="brnQ"
                     /locus_tag="ACZPCG_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HIR1285849.1"
                     /translation="MTIQKLSIQNLLLVGFTLFSMFFGAGNLIFPPDLAARAGAQVIP
                     AMAGFLISAVGLPVLGVAAVAVSGGLEKLAGKVGGRFSLLFTLTAYLAIGPFLAIPRT
                     ASTSFEMTLLPVLSGAGVQLGDYLGHTGMTVQFWAQLGYSFVFFAIALLLSVHPETLS
                     ERLGKVTCPALLILIAVLFTGRLIWPMEGVGETVAEFAAGASVRGFMEGYQTMDTLAA
                     LNFGIIISLNIRNKGISREDSVIRETIKAGLMAGILLSLVYGALAYIGGPVGQQAGAG
                     TNGARILTFVAGDLFGISGMVILGIIFFIACLNTCVGLICCCSQFFSTLAPCVGYRIW
                     TVVFAVISLLISSAGLDRILKYSVPVLNAVYPIAIVLIALAFMERFTGRRIWVYRLSI
                     LFTAAVSVVWTMDQAALAIPGITEGMRHLPGYAPGLGWLLPAVIGGLLGRMIPGGNET
                     G"
     gene            1579..2040
                     /locus_tag="ACZPCG_12240"
     CDS             1579..2040
                     /locus_tag="ACZPCG_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HIR1285850.1"
                     /translation="MTNEQYYSLIQSYEDARKILISRLEILDHSLYGNHTESGPVHTI
                     QSRIKSKDSIEEKLKRSGKTNSIANARDYLLDIAGIPVYCLDTMEYFPVEVQLRTMSM
                     DFWASMEHRICYKKNPHGREQLSRDFKIYADILEEIERQLEGYSDVKKKIE"
     gene            complement(2085..2158)
                     /locus_tag="ACZPCG_12245"
     tRNA            complement(2085..2158)
                     /locus_tag="ACZPCG_12245"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2122..2124),aa:Arg,seq:acg)
     gene            2421..3848
                     /locus_tag="ACZPCG_12250"
     CDS             2421..3848
                     /locus_tag="ACZPCG_12250"
                     /inference="COORDINATES: protein motif:HMM:NF012686.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HIR1285851.1"
                     /translation="MVLQTQALIIVAAYLIGMLLVGFIVGKLKIKDSSDYMLAGRRMG
                     LFMVAFSLSANNIGGGSTTGLAQKAYGEWGMSAIWYVLTASIAMIPLAYFAPKIRKTM
                     AVTIPEVVGRRFGKTSSTLTAVLSILSLFCLTSSQIAASGTVVASLTGIPTNVALLIA
                     GAVVILYTTFGGMIADQISDLVQFGIILIGLAVATPIVIHGCGGWDAISAALPPAHLS
                     FTKIGWVTIIGYIFNYFCTFLAGPEMVSRFETAKSEKVAKEAAWLSAVLMAAMSIFPT
                     LLGLAALATMPELADGGKNAMMAVTSAHAPGAIVGIISAAIICATMSSADSNLLCMST
                     MLMNDLYLKYGGKQLKDKQVIAYTRTCNVVAAIIAMIISMAGIPIVTMNTFAFGIRCA
                     GPFAAYGLGLIVPKATKNSGILSLIGGTIGFCFWQYLSGGGFKFGILPVVFGCAVSVA
                     VFFLVNAIEWSRGVEPAPSAYMDEK"
BASE COUNT         1045 a         1030 c          846 g          977 t
ORIGIN      
        1 cccccgtttc atttcctccg ggaatcatcc ttccaagcag cccgccaatc acagccggca
       61 gcagccatcc aagaccggga gcatagcctg gaagatgcct cataccttcc gttatgcccg
      121 gaatcgcaag agctgcctga tccatggtcc agacaacgct taccgccgcc gtaaacagga
      181 tcgacagacg gtacacccag atcctgcgcc ctgtaaaccg ctccataaaa gctagagcaa
      241 tgagaacaat cgcgatcgga taaacggcat ttaaaacagg aacggaatat ttcaaaatac
      301 ggtccagtcc cgcgctggaa atcaaaaggc tgataactgc aaaaaccacc gtccatatcc
      361 gatatccaac gcatggcgcc agagtagaaa aaaactggct gcagcagcag atcagtccca
      421 cacaggtatt caggcaggcg ataaaaaaga taatccccaa aatcaccatt cccgaaatgc
      481 caaaaagatc tccggccaca aaggtcagga ttctggctcc gttggtgcct gctcccgcct
      541 gctgtcctac agggcctcca atataggcca aagcaccata gaccagagat aaaaggatcc
      601 ccgccataag tccggccttt atcgtttccc ggattacgga atcctctctg gaaatcccct
      661 tatttcttat attaagagaa atgataatgc caaaatttaa agccgcaagc gtatccatgg
      721 tctgataccc ttccataaag cctctcaccg aagctcctgc cgcaaattct gccactgtct
      781 cgcccactcc ctccatgggc cagatcagcc ttcccgtaaa taatactgca atcaggatca
      841 acagtgccgg acaggtcacc ttgcccaaac gttctgaaag ggtttccgga tgaacggaca
      901 aaagaagggc aatggcaaaa aacacaaagg aatatcccaa ctgagcccaa aactgaaccg
      961 tcatgcccgt atggcccaga taatctccca gctggactcc cgcaccgctg agaacaggaa
     1021 gcagcgtcat ctcaaaggaa gtgcttgcag ttctgggaat cgccaggaag gggccgatcg
     1081 ccagatacgc ggttaaagta aataaaagcg aaaatctccc gcccactttt cccgccagct
     1141 tttcaagtcc tcctgaaaca gccaccgcag ccactcccag aaccggaagc cccactgcgc
     1201 tgatcaaaaa gccggccata gccggaatca cctgagcgcc tgcccgtgca gccagatccg
     1261 gaggaaagat cagatttccc gcaccgaaaa acatcgaaaa taaagtaaat cctaccagaa
     1321 gcagattctg gatggaaagc ttttgtattg tcataaatct ccacctgttc tgtatccgat
     1381 tgcgtcattt tttatcgccg ctgcctgcaa aaatatctgc agagcttctt atcatcatac
     1441 catattccgg ggcaggtttc aactgcggca aaattcctgc aggtaaaatt cctacacagt
     1501 ttttacacaa ttctgtcaca gtttaatcgt attctattct ggtatatgaa ctacattaaa
     1561 gtgagggaac accagattat gaccaatgaa cagtattaca gcctgatcca gtcctatgag
     1621 gatgccagaa aaatcctgat ttcccgactg gaaatactgg atcacagcct gtatgggaac
     1681 cataccgaaa gcgggcctgt ccataccatc caaagccgga tcaagtcaaa agacagtatt
     1741 gaggaaaagc ttaagcgctc gggaaaaaca aacagcattg ccaatgccag agattatctg
     1801 ttagatatcg ccggaatccc ggtttattgc cttgatacaa tggaatattt tcccgtagag
     1861 gttcagctgc gcaccatgtc catggacttc tgggccagta tggaacaccg tatctgttac
     1921 aaaaagaatc ctcatggccg ggaacagctg agccgggatt ttaaaattta tgcggatatt
     1981 ttggaagaaa tcgaacgaca gctggaaggc tacagtgatg taaagaaaaa gatagaataa
     2041 ttgcaaaaaa cctcgtaagc aaaacagcta cgaggttttt atcatgcgcc agagaagatt
     2101 cgaactcccg acactacggt ccgtagccgt atgctctatc cagctgagct actgacgcat
     2161 ctttcattac tttggcttgt ctgcctcagc aacgtagatt attatatgcg ataaaactca
     2221 aaatgtcaac tacttttttc atttatttta ttttttgttt tttgtcagtt ttacatctcc
     2281 ataaaaagtt gttttccgac agcttcagca gatattttct ttcatttgat ttattttgac
     2341 acagaatctt tatttacatt cttttctgaa tcatttataa tttatttata ttttttttaa
     2401 tattttaagg agggtttctt atggtactgc aaacgcaagc tcttattatc gtagctgcct
     2461 atctgatcgg catgctgttg gttggcttca tcgtggggaa actgaagatc aaagacagca
     2521 gcgactatat gctggccgga cggcgtatgg gactttttat ggtggcattc tcattgtcag
     2581 ccaacaatat cggcggcggt tctactacag gactggcgca gaaagcctac ggcgaatggg
     2641 gaatgagtgc gatctggtat gtactgaccg catccatcgc tatgatccca ttggcttatt
     2701 tcgccccgaa gatccgcaaa accatggctg tgaccattcc tgaggttgta ggacgcagat
     2761 tcggaaaaac atccagtact ctgactgcgg ttttgagtat cctgtccctt ttctgcctta
     2821 cctcatccca gattgcagcc tccggcactg tagtagccag cctgaccgga attcctacca
     2881 atgtagctct gctgatcgca ggcgctgtag tgattctcta tactaccttt ggcggcatga
     2941 tcgcagacca gatttcagat ttggtccagt tcggcatcat actgatcggt cttgctgttg
     3001 ccacccccat cgtaatccac ggatgcggcg gatgggacgc catttctgcg gctcttcctc
     3061 ctgctcattt aagctttacg aaaatcggct gggtaacgat catcggatac atcttcaact
     3121 atttctgcac cttcctcgca ggccctgaga tggtatcacg ttttgaaact gcaaaaagtg
     3181 aaaaggtcgc aaaggaagcc gcctggcttt ctgctgtact gatggcggcc atgtccattt
     3241 tcccgacact tctgggcctg gcagctctgg ctaccatgcc ggaactggcc gacggcggaa
     3301 aaaatgccat gatggccgtt acctccgccc atgctcccgg agccatcgtc ggcatcatct
     3361 ctgccgctat catctgcgcc accatgtcct ctgcggactc taatctgtta tgtatgtcca
     3421 ccatgctgat gaatgacctg tatctgaaat acggcggcaa acagcttaag gataagcagg
     3481 ttatcgccta cacccgtacc tgcaacgttg tggcagcaat cattgccatg atcatctcca
     3541 tggcaggaat ccccatcgta actatgaaca cctttgcctt tggcatccgg tgcgcgggcc
     3601 cctttgcagc ctacggactt ggcctgatcg taccaaaggc gactaaaaat tccggtattc
     3661 tctctttgat cggcggaacg atcggtttct gcttctggca gtatctcagc ggcggaggct
     3721 ttaaatttgg cattcttcct gtggtattcg gctgtgcagt cagcgtagct gtatttttcc
     3781 ttgtaaatgc catcgaatgg agccgcggtg tagaacccgc cccctctgcc tatatggatg
     3841 agaaataatc tgttctgaaa ccagaattac aaaaagcgcc gtcgcttcct atctgcta
//
LOCUS       DBMDUH010000194         3886 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_8377_length_3886_cov_4.891413, whole genome shotgun sequence.
ACCESSION   DBMDUH010000194 DBMDUH010000000
VERSION     DBMDUH010000194.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3886)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8377_length_3886_cov_4.891413"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..2029
                     /locus_tag="ACZPCG_12255"
     CDS             <1..2029
                     /locus_tag="ACZPCG_12255"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HIR1285852.1"
                     /translation="QQFADALKIRGRRGDGYIESEDAIVTWCVGHLVTMSYPDAYDMK
                     FKRWSLQTLPFLPKEFKYEIIPGVARQFAVVKGLLNRPDVDTIYVCTDSGREGEYIYR
                     LVAQMAGVKDKCQKRVWIDSQTEEEILRGIREAKDESEYDNLSASAYLRAKEDYLMGI
                     NFSRVLTLRYGPAMSNYLGTKFTVLSVGRVMTCVLGMVVRREREIRSFVKTPFYRVIG
                     TFDAVAKDGRALPLEAEWKAVEGSRYFGTPCLYKDNGFKEEERARELIAYLKEGEPLT
                     AAVVSKEKKKETKNPPLLYNLAELQNDCSKMFKISPDEALKVVQELYEKKMVTYPRTD
                     ARVLSTAVAKEIRKNISGLSGYSPMRAFVQEILQGSSWQNLAKTRYVNDKQITDHYAI
                     VPTGQGLGSLRQLTPLQEKVYQVICRRFLSIFYPPAVYQKYTLELERKKEHFFAGFKV
                     LSEPGYLKVADVNLAKKPGVEETFEDQKEENPDKNNTESPKVDRTELLQVLADLKKND
                     ILTVADLRVKEGETSPPKRYTSGSMILAMENAGQLIEDEELRAQIKGSGIGTSATRAE
                     ILKKLVSIQYLSLNGKTQVITPSLLGEMVYDVVDHSIRQLLNPELTASWEKGLTYVAE
                     GFITSGEYMEKLERFVAGRTVNVIRLNNQYDLRGLFDAAAVFYKQKRRTDQ"
     gene            2026..2817
                     /locus_tag="ACZPCG_12260"
     CDS             2026..2817
                     /locus_tag="ACZPCG_12260"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HIR1285853.1"
                     /translation="MKLEEMKMKELFDMSHTIAERIFDNHAYPWEVLPEIGDFIRSLG
                     SLLPENEYRKIGKDIWIHKTAKVAPTIMMTGPMIICAGANVRHNAFLRGNVIIGEKAV
                     VGNSCELKNALLFDEVQVPHFNYVGDSILGYKAHMGAGAVTSNVKSDKSLVKVHAEDG
                     DVTTGFKKFGAILGDCVEVGCNSVLNPGTVIGKNSVVYPLSSVRGCVAANSIYKNQEN
                     VIVKENRDAEAEAVKAEAEEPAPKPKRTRAKKSTAASSSTVKVVK"
     gene            complement(2917..3546)
                     /gene="upp"
                     /locus_tag="ACZPCG_12265"
     CDS             complement(2917..3546)
                     /gene="upp"
                     /locus_tag="ACZPCG_12265"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HIR1285854.1"
                     /translation="MEQIMVFNHPLIQHKISILRNKSTGTNEFRALIEEIAMLMGYEA
                     LRDLPLEDVEVETPLETCMTPMIAGRKLAVVPILRAGLGMVNGILALVPSAKIGHIGL
                     YRDEVTHEPHEYYCKLPSPIEERTIIVTDPMLATGGSAVSAVDFIKQHGGKKIKFMAI
                     IAAPEGLERLHKAHPDIQIYVGHLDRCLNEDAYICPGLGDAGDRIFGTK"
     gene            complement(3645..>3886)
                     /locus_tag="ACZPCG_12270"
     CDS             complement(3645..>3886)
                     /locus_tag="ACZPCG_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285855.1"
                     /translation="DVRFPKLNAEETSAALEYRSTQSMEALTAVFENTDFPLSGKETW
                     PESFSYEKQGRIYFSLPIPHALGDYVIVEFDPATK"
BASE COUNT         1094 a          827 c         1049 g          916 t
ORIGIN      
        1 ccagcagttt gcagatgcat taaagatccg gggacggcgg ggagacggat atattgaatc
       61 cgaggacgcc attgtaacct ggtgtgtggg ccatctggtg acgatgagct atccggatgc
      121 ctatgacatg aaatttaaac gctggagcct gcagaccctg ccgtttctgc cgaaggaatt
      181 caaatacgag attattcccg gcgtagccag gcagtttgca gtggtaaagg ggcttttaaa
      241 ccgtccggat gtggatacga tctatgtctg cacggactcc gggcgggaag gagaatatat
      301 ctaccgtctg gtggctcaga tggcaggagt aaaagacaag tgccagaaac gtgtgtggat
      361 cgattcccag acagaggagg agatcctcag gggaatccgg gaagcaaagg atgagtcgga
      421 atatgataat ctttctgctt ctgcctatct gcgcgcaaag gaagattacc tgatggggat
      481 caacttctcg cgggtgctta ccctgcggta cggtcctgcc atgtcaaact atctgggaac
      541 caaatttacg gttctttccg taggtcgtgt gatgacctgt gtgctgggaa tggttgtccg
      601 cagggagagg gagatacgaa gctttgtaaa gactcccttt tatcgtgtga tcggaacctt
      661 tgacgcagtg gcaaaggatg gccgagccct tccgctggag gctgaatgga aggcggtgga
      721 aggttccaga tattttggaa cgccctgcct ttataaggac aacggcttta aggaggaaga
      781 acgggccaga gaactgatcg catatctaaa ggaaggggaa ccgcttacag ctgccgttgt
      841 ttcaaaggaa aagaaaaagg aaacaaaaaa tccgccgctt ctttacaatc tggcagagct
      901 tcagaatgac tgctcaaaaa tgtttaagat cagcccggat gaagcgctga aggtcgttca
      961 ggagctgtat gaaaaaaaga tggttaccta tccccgtacg gatgccaggg ttttatccac
     1021 ggcggtagca aaagagatcc gaaaaaacat aagcggactc tccggctaca gccccatgcg
     1081 tgcctttgtt caggagattt tgcagggaag cagctggcag aacctggcaa aaaccaggta
     1141 tgtcaacgac aaacagatca cagaccacta tgcgatcgtt cctacgggcc agggactggg
     1201 cagcttaaga cagcttacac ccttgcagga aaaggtctat caggtgatct gccgccgttt
     1261 tttaagtatt ttttatccgc ctgccgttta ccagaagtat accctggagc tggagcggaa
     1321 aaaggagcat ttttttgcgg gatttaaggt gctttccgag cccggatatt tgaaggtggc
     1381 ggatgtgaat ctggcaaaaa aaccaggggt tgaagagacc tttgaggacc agaaagagga
     1441 gaatcccgat aaaaataata cagaatcccc aaaagttgac agaacagaac tgttacaggt
     1501 gcttgcagat ttgaaaaaaa atgatatact tactgtagcg gacctgcggg tgaaggaagg
     1561 ggaaacctca cctcccaagc gatatacctc aggctctatg atcctggcga tggaaaatgc
     1621 gggccagctc attgaggatg aggagctgcg ggcccagatc aagggaagcg gtatcggtac
     1681 cagcgccacc cgcgcggaga ttttaaaaaa actggtgagt atccagtatc tttctttaaa
     1741 tggaaagaca caggtgatca cgccgtccct tttaggggaa atggtgtatg atgtggtgga
     1801 tcattccatc cgccagcttt taaatccgga gcttacagcc agctgggaaa agggattgac
     1861 ctatgtagcc gagggtttca ttacctccgg cgaatatatg gaaaagctgg aacgctttgt
     1921 agcaggaaga accgtcaatg tgatccgcct gaacaatcag tacgatctga gaggactttt
     1981 tgacgcggct gctgtatttt ataaacaaaa aaggagaact gatcaatgaa attggaagaa
     2041 atgaagatga aagagctttt tgacatgagc cataccattg cagaaaggat ctttgacaat
     2101 cacgcatatc catgggaggt actgccggag atcggcgatt tcatccgttc tttgggatcc
     2161 cttcttcctg aaaatgagta tcggaagatc ggtaaagata tctggatcca taaaactgca
     2221 aaggttgcgc ctacgattat gatgacaggc cctatgatca tatgtgcggg agccaatgtg
     2281 cgccacaacg catttttaag aggcaatgta atcatcggtg aaaaggctgt agtaggaaac
     2341 tcctgtgagt taaagaatgc cctgctgttt gacgaggttc aggtacctca cttcaactat
     2401 gtaggcgatt ccatcctggg ctacaaggca catatgggcg caggcgcagt gacctctaat
     2461 gtaaagtctg acaagagcct ggtaaaggtt cacgcagagg acggcgatgt gacgactgga
     2521 tttaagaaat tcggcgcaat cttaggagac tgtgttgaag ttggctgcaa cagcgtatta
     2581 aatccgggta ccgtaatcgg aaaaaacagc gttgtatatc ctctttccag tgtaagaggc
     2641 tgtgtagcgg caaattccat ttacaagaat caggaaaatg tgatcgtaaa ggaaaacaga
     2701 gatgcagaag ctgaagccgt taaggcagag gcagaagagc ctgctccaaa gccaaagaga
     2761 accagagcaa agaagagcac agcagcttcc agcagcactg taaaggtagt aaagtaaata
     2821 gaagataaga tttaacgcat gatgcgcata aaaagacctc ctctcaatcc gggaaaacct
     2881 gtgaaagaga ggaggttttt tatgatggga acgggattat tttgtgccga aaatacggtc
     2941 gcctgcatcg cccagacccg gacagatata ggcatcttca ttcaggcagc gatccagatg
     3001 gcctacatag atctggatat ctggatgtgc cttgtgaagg cgctctaagc cctcgggagc
     3061 agcgatgatc gccataaatt tgattttttt cccgccatgc tgtttgatga aatcaacagc
     3121 agacacggcg gagcctccgg ttgccagcat gggatcggtg acaatgatgg tgcgctcctc
     3181 aatggggctt ggaagcttgc agtaatactc atgaggctca tgggttacct cgtcgcggta
     3241 aaggccgata tggccgatct ttgcgctggg aaccagggcc agaatgccgt ttaccatgcc
     3301 aaggccagcg cgcaggatgg gaactacagc cagcttgcgt ccggcgatca taggcgtcat
     3361 gcaggtctct aaaggtgtct ctacctcaac atcctctaaa ggaagatctc ttaaagcctc
     3421 gtatcccata agcatggcaa tctcttcgat cagggcgcgg aattcattcg ttccggtact
     3481 cttgttgcgc aggattgaaa ttttgtgctg aatcagcgga tgattaaaaa ccattatctg
     3541 ttccataaaa gcagtgctcc tttgtatatt ttttaacgta cattcattat actggataaa
     3601 aggccggggt gcaatggaaa actcaaggaa ttatcgttta acgcttattt ggtggccgga
     3661 tcaaattcta cgatcacata gtctcccaga gcatggggga tgggaagaga gaagtaaatc
     3721 cggccctgtt tttcatagct gaaggattcc ggccaggttt ctttcccgga aagcgggaaa
     3781 tctgtatttt caaaaacagc ggtcaatgct tccatagact gtgtgctccg gtattccaag
     3841 gcagcgcttg tttcctcggc attcagcttt ggaaaacgta catctt
//
LOCUS       DBMDUH010000195         3858 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_8451_length_3858_cov_5.945169, whole genome shotgun sequence.
ACCESSION   DBMDUH010000195 DBMDUH010000000
VERSION     DBMDUH010000195.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8451_length_3858_cov_5.945169"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="ACZPCG_12275"
     CDS             complement(<1..248)
                     /locus_tag="ACZPCG_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIR1285856.1"
                     /translation="MINSTSNKQVKRVVNLQAKAKARREEGVYVAEGLRMCREFTPKD
                     VEALYVTPSFESQKENREWLHSYSYEIVTEEVMKAMADT"
     gene            complement(245..2221)
                     /locus_tag="ACZPCG_12280"
     CDS             complement(245..2221)
                     /locus_tag="ACZPCG_12280"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006780948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HIR1285857.1"
                     /translation="MKDGFLRTAAATPKIRVADPQYNAKQIIELMEQGESRGVKVMVF
                     PELCLTGYTCSDLFLQASLLESVKRELKHIVEASKKLHMIAFVGLPWEHEGKLYNAAA
                     AISGGRILGLVPKKNLPNYSEFYELRHFCPGQETVRWIKIDGERVPFGMNLLFSCEDI
                     PGLVLAAEICEDVWVPCPPSISHAMAGATVIANCSASDETTGKDAYRRSLICGQSARL
                     VCGYIYANAGEGESTQDLVFGGQNILAENGTCLMESRRFSNECICADMDLERITAERR
                     RMTTFPAADPYQEKGGYVTVPFELERGAKEAEGSILRYVDAAPFVPADPVQRERRCEE
                     ILSIQAMGLKKRLEHTGCKNAVIGLSGGLDSTLALLVTVRAFDLLNLPREQIHCITMP
                     CFGTTDRTYTNACVMAKKVGASLKEINIRKSVTSHFEDIGQDVNCHDVTYENGQARER
                     TQILMDISNQVGGLVIGTGDMSELALGWATYNGDHMSMYGVNASVPKTLVRHLVRYYA
                     DTCGEKELSDVLLDVLDTPVSPELLPPEDGKISQKTEDLVGPYELHDFYMYYVLRFGF
                     RPGKIYRLALCAFEGQYDRETILKWLTTFYRRFFSQQFKRSCLPDGPKVGSVAVSPRG
                     DLRMPSDAVSRLWLDELKELELSGTGNGKGARAK"
     gene            2444..3217
                     /locus_tag="ACZPCG_12285"
     CDS             2444..3217
                     /locus_tag="ACZPCG_12285"
                     /EC_number="6.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009460569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_function: GO:0061503 - tRNA threonylcarbamoyladenosine
                     dehydratase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HIR1285858.1"
                     /translation="MITEFSRTEMLIGPKALNALKGSCVVVFGAGGVGSHAIEALARS
                     GIGHLILIDSDTVSLTNINRQSIAYHSTVGQYKTRVMADRIRDICPDTRTDTFEEFVL
                     PDTMETLFCRINALLEEEHIKKSQISSSGSSSITYILDAIDTVSAKIALAAYADEHSI
                     PLISSMGTGNKLHPELFRISDLKDTSVCPLCRVMRKELKTRGIQSLKVCWSPEKPLTP
                     APAEEDTGSRRSTPGSISFVPPAAGLMIAGEIVRNICGL"
     gene            complement(3224..3643)
                     /locus_tag="ACZPCG_12290"
     CDS             complement(3224..3643)
                     /locus_tag="ACZPCG_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HIR1285859.1"
                     /translation="MLVSTKGRYALRTMIDLALHGSGEPVKIKDIARRQDISGKYLEQ
                     IISILSRAGCVRSVRGNQGGYYLTRPASEYTVGMILRITEGNLAPVDCLAGEDNPCQR
                     RDDCVTLRLWQELDDAIRGVVDQYTIEDLARWQEAAK"
BASE COUNT          932 a         1099 c          842 g          985 t
ORIGIN      
        1 gtatccgcca tggccttcat aacttcttcg gtgacaattt cgtaggaata ggagtgaagc
       61 cactccctgt tttccttctg gctctcaaag gacggcgtaa cataaagagc ctctacgtct
      121 tttggtgtaa attcccggca catccgcagt ccttctgcca catagacgcc ctcttcccgc
      181 ctggcttttg cctttgcctg aagatttaca acccgcttta cctgcttatt tgaagtgctg
      241 ttgatcattt tgctctggct cctttcccgt ttccggtccc ggacagttcc aactccttaa
      301 gttcatccag ccacagacgg cttacggcat cgcttggcat acgcagatct cctctgggag
      361 atacggccac agagcccacc ttcggtccat ccggcagaca ggagcgctta aactgctggc
      421 tgaaaaatct tcggtaaaag gtggtaagcc attttaaaat agtctcccta tcatactgtc
      481 cttcaaacgc gcaaagagcc agacggtaaa ttttacccgg acgaaagccg aaacgcagta
      541 catagtacat ataaaaatcg tgaagctcat aagggcctac caggtcctcg gtcttttggg
      601 atattttccc atcctcaggc ggaagaagct ccggactcac aggcgtatca agtacatcca
      661 ggagcacatc tgaaagctct ttttccccgc aggtatctgc ataatagcgg accagatggc
      721 ggaccagtgt cttgggaaca gaagcattga ccccgtacat ggacatgtgg tctccgttat
      781 aagtggccca gcccaaagca agctcggaca tatctccggt tccgatcaca agccctccta
      841 cctgattgga aatatccatc aggatctggg tacgctctct ggcctgtccg ttttcgtagg
      901 ttacatcatg gcagtttacg tcctgtccaa tatcctcaaa atggcttgta acggacttgc
      961 ggatattgat ttccttaagg gatgccccta ccttctttgc catcacgcag gcgttggtat
     1021 aggtacggtc cgtagtgccg aagcagggca tggtaatgca gtggatctgt tctctgggaa
     1081 gatttaaaag gtcaaaggcc cgaaccgtca ccaaaagagc cagcgtggaa tccagaccgc
     1141 cggaaaggcc gatgaccgca tttttacagc ccgtatgctc cagacgcttc tttaagccca
     1201 tagcctgaat ggacagaatc tcctcacagc ggcgttctct ctggacagga tccgcaggca
     1261 caaagggagc cgcatctaca tagcgcagaa tggagccctc cgcttctttc gctccccgct
     1321 ccagctcaaa cggcacagtc acatagccgc ctttttcctg atacggatct gctgccggga
     1381 aggttgtcat cctgcggcgc tccgctgtga tccgttccag atccatatcc gcacagatac
     1441 attcattgga aaagcgccgc gattccatca ggcaggttcc attttccgcc aggatattct
     1501 gtccgccgaa taccagatcc tgggtggatt ccccttctcc tgcattggca tagatatatc
     1561 cgcagaccag acgggccgac tggccacaga tcaggctcct gcggtaagca tcctttcctg
     1621 tggtttcatc gctggcagag cagtttgcga tcaccgtggc tcctgccata gcatgagaaa
     1681 tgctcggagg gcagggaacc cacacgtcct cgcagatttc cgctgccagc acaagtcccg
     1741 gaatatcctc acaggaaaac agaaggttca tgccaaaagg aaccctttcc ccgtcaatct
     1801 ttatccagcg taccgtttcc tgtccgggac agaaatgacg cagctcataa aattccgaat
     1861 agttgggcag attctttttg ggaaccagtc ccaggatcct tcctccgctt atggcagctg
     1921 ccgcattgta aagctttccc tcatgctccc agggaagacc cacaaacgcg atcatatgca
     1981 gcttctttga tgcttctacg atatgtttca gttccctttt tactgattcc aaaagggaag
     2041 cctgcaaaaa aagatccgaa caggtgtagc ctgtaagaca cagttccgga aataccatca
     2101 cctttactcc ccggctctct ccctgttcca tcagctctat gatctgtttt gcattatact
     2161 gcggatctgc caccctgatt ttaggagtag cagctgcagt ccttaaaaat ccgtctttca
     2221 tcttgacctc cagctcttat gcatagaaat tttctgaccg tagtatagca tagatccgca
     2281 taaaatccca gagtgaattt tctctgttct tcccttgact attcataaaa tctgctgtat
     2341 acttaggctg ttcagaacgc ctgttctgta tgttttataa agccctgcac cactactacc
     2401 acacttgcca gggctttatt tttttaccaa aaggaggttt tccatgatca ctgaattttc
     2461 acgcacggaa atgctgatcg gccccaaggc cctgaatgcc ttaaaaggat cctgtgtggt
     2521 tgttttcgga gcaggaggcg taggctctca tgccattgaa gccctggcca ggtctggtat
     2581 cggccatctg atccttatag acagcgatac ggtttccctg acaaatataa accgccaaag
     2641 cattgcttat cacagcactg tgggccagta taaaaccaga gtaatggcag accggatacg
     2701 ggatatctgc cctgatacaa ggacagatac ctttgaagaa tttgtgctcc ctgatacaat
     2761 ggagacgctt ttttgccgga tcaatgccct tttagaagaa gaacatatta aaaaaagcca
     2821 gatttcttcc tccggctctt cttccattac ctatatttta gatgccattg acaccgtttc
     2881 cgccaagatt gcacttgctg cttatgcaga cgagcactcc atcccactga tctcatccat
     2941 ggggacaggg aacaagcttc accccgaact gtttcgtatc tctgatttaa aagacacttc
     3001 ggtctgcccc ctgtgccgtg tgatgcgcaa ggagctgaag acaagaggca ttcaaagcct
     3061 gaaagtctgc tggtccccgg aaaagcctct gacgcccgcg cctgctgagg aagatacagg
     3121 cagccgccgc tccacccccg gaagcatatc ctttgtgcct cccgcagcag gccttatgat
     3181 cgccggagaa atcgtaagaa atatctgcgg cctctaacaa aacctacttt gcagcctcct
     3241 gccaccgggc cagatcctca atggtatact ggtctaccac accccggatt gcatcatcca
     3301 gctcctgcca cagccgcagg gtgacgcagt catcccttcg ctggcagggg ttgtcctcac
     3361 cggccagaca gtctaccgga gccagatttc cttctgtgat ccgcaggatc atccctacgg
     3421 tatactcgct ggccggccgg gtcaaataat agcctccctg atttccacgg acacttctca
     3481 cacagccggc ccgggacagg atcgatatga tctgctccag atactttcct gaaatatcct
     3541 gacgccttgc aatatctttt attttcaccg gctccccact cccatgaaga gccagatcga
     3601 tcatcgttct taacgcatac cgcccttttg tggatacaag catataaaaa cagtcctttt
     3661 ctttttattt gggtttgggc atctggcagt atgcccaaac ccatatttga ttcatgccct
     3721 tatcatacta ttcctgtagg aaaaatgtca atagtatttt ccggaaatac aagaagagct
     3781 gcccttcccc cacagaaggc agctctcagt cacgatcaaa aaccgtcaca ctataagttg
     3841 tgctggcatc cccatacc
//
LOCUS       DBMDUH010000196         3801 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_8600_length_3801_cov_4.661622, whole genome shotgun sequence.
ACCESSION   DBMDUH010000196 DBMDUH010000000
VERSION     DBMDUH010000196.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3801)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8600_length_3801_cov_4.661622"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1211
                     /locus_tag="ACZPCG_12295"
     CDS             <1..1211
                     /locus_tag="ACZPCG_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006780534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HIR1285860.1"
                     /translation="PNNSHAKEKIRKQVVADMDSVGYIEKPLVPYIKVTQDRVVLEIQ
                     RGCIRGCRFCQAGNVYRPLREHSLEYLKHYAYDMLKSTGHEEISLSSLSSSDYTYLEG
                     LVNFLIDEFKGKGVNISLPSLRIDAFSLDVMSKVQDIKKSSLTFAPEAGSQRLRDVIN
                     KGLTEEVILKGASEAFQAGWNRVKLYFMLGQPTETVEDMEGIALLSEKIAEAYYDIPK
                     DQRNGKVQVVASSSFFVPKPFTPFQWARMCTKEEFLERANIVRGKFREMKNFKSLKYN
                     WHEAELTVLEGVLARGDRRVGPVIEEAYKNGAIFDSWSEFFKNEIWMDAFKTCGVDID
                     FYTTRERGLDELFPWDFIDAGVTKEFLKREWQQALNGEVTPNCRQKCSGCGAMGFGGG
                     VCFETRGKNQ"
     gene            1208..1957
                     /locus_tag="ACZPCG_12300"
     CDS             1208..1957
                     /locus_tag="ACZPCG_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008398297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HIR1285861.1"
                     /translation="MKIRIKFRKTGIMKFIGHLDVMRYFQKAIRRADVEICYSGGFSP
                     HQIMSFAAPLGVGITSNGEYVDIEVHSTGTTAEMLERLNAVMAEGFEIAEYKLLPDTA
                     ANAMSSVAAADYTLTFRPGYEPEEESAEEWFKKLTAFFDQPQVMVLKKTKKGEKEMDL
                     KPLIYDLGVIAGNDAAQSQLFMKISTGSASNIKPELLLDAYYEALGKERSPFAFMVQR
                     EEVYADQASEHEREQGIHSFIPLGALGQDIE"
     gene            1957..3168
                     /locus_tag="ACZPCG_12305"
     CDS             1957..3168
                     /locus_tag="ACZPCG_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HIR1285862.1"
                     /translation="MEKRIITWLRGRICTAFIQDGSLVELTLEDEDCSVLNRIYVGRV
                     QKVVPGINAAFIDTGDGVGYYSLTENRSHLFTDRNRKEGPLRQGDEILVQISREGVKT
                     KAPVLTANLSLPGRLCVVTAGRTGIGFSNKISDSLFKKEAKACLEEAPLSGFGVIVRT
                     NAYQAGMDALIKECILLKERLEALIKDAACRVSGSCLYQPLPSYITGVRDAYEGRLSS
                     IVTDDRGLYDQLKAYLKEEQPEDLEKLEFYDDSLLPLYKLYGLETVLDRALGKRVWLK
                     SGGYLVIEPTEAMTVIDVNTGKYEGRKKMADTIRKINNEAAFTIAAQLRLRNLSGIIM
                     VDFIDMEQEEDKKALLDCLKQAVSTDPVRTVVVDMTPLGLVELTRKKVRKPLYEQAGQ
                     TYDGMRGDKNL"
     gene            3165..>3801
                     /locus_tag="ACZPCG_12310"
     CDS             3165..>3801
                     /locus_tag="ACZPCG_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HIR1285863.1"
                     /translation="MKIIIVGCGKVGTTLAEQLNRENHDITLIDTNEEAIQNISDSAD
                     VMGVTGNGAVYQVQMEAGIQDADLLIATTNSDELNMLCCLIAKKAGNCHTIARIRNPE
                     YSSEIRYIREELNLSLAINPELAAAREIARLLRFPSAIKIEPFAKGRIELLKFLIPEH
                     SLLNDMRVMDVVNRLKSNVLICVVERGNDVVIPDGNFVMKKGDKISFIASHQ"
BASE COUNT         1030 a          831 c         1030 g          910 t
ORIGIN      
        1 cccccaacaa cagccacgca aaagagaaga tccgcaagca ggtcgttgcc gatatggaca
       61 gtgtgggtta tatcgaaaag cctctggttc cctatataaa ggtcactcag gaccgcgtgg
      121 ttctggagat tcagagaggc tgcatccggg gctgccgttt ctgccaggcc ggaaacgtat
      181 accgtccctt aagagagcac agcctggaat atttaaagca ttatgcctac gatatgctta
      241 agagtacagg ccatgaggaa atttctttaa gctccttaag ctccagcgac tatacctatc
      301 tggagggcct tgtgaatttc ctcattgacg agtttaaggg gaaaggggtc aatatttccc
      361 tgccttcctt acgaatcgac gccttctccc tggatgtcat gagcaaggtt caggatatca
      421 aaaagagcag ccttaccttt gcgcctgagg ccggctctca gcgtctgcgg gacgtaatca
      481 ataagggact gaccgaagag gtgatcttaa agggcgcttc cgaagccttc caggccggat
      541 ggaaccgggt aaagctttac tttatgctgg gacagcctac agagaccgta gaagatatgg
      601 agggaattgc cctgctgtct gaaaagatcg cagaagccta ttatgacatt ccaaaggatc
      661 agagaaacgg aaaagtccag gtagttgcca gttcctcctt ctttgttcca aagccattta
      721 cccccttcca gtgggccaga atgtgcacga aagaggagtt tttagagagg gccaatattg
      781 tccgtggcaa attccgtgaa atgaaaaatt tcaaaagctt aaaatacaac tggcatgagg
      841 cagagctgac tgttcttgag ggcgtccttg ccagaggcga ccgccgggta ggccctgtga
      901 tcgaggaggc ctacaaaaac ggtgccatct ttgattcctg gtccgagttc tttaaaaatg
      961 agatctggat ggatgcattt aagacctgcg gcgtggacat tgatttttac accaccagag
     1021 agcgcggcct ggatgaactg ttcccctggg attttattga tgcaggcgtg acaaaggaat
     1081 ttttaaagag agaatggcag caggctttaa atggtgaggt aacgcccaac tgccgccaga
     1141 aatgttccgg ctgcggagcc atgggctttg gtggcggcgt atgttttgaa accaggggga
     1201 aaaaccaatg aaaatccgta ttaagttcag aaaaacaggt attatgaaat tcatcggcca
     1261 cctggatgtg atgcgctatt tccagaaggc catccgaaga gccgatgtgg agatctgcta
     1321 cagcgggggt ttcagccctc atcagatcat gtcctttgca gcgcctttag gcgtgggaat
     1381 caccagcaac ggcgaatatg tggatattga agtacactcc accggcacca cggctgagat
     1441 gttagagcgt ttaaatgccg ttatggccga gggctttgag atcgcagagt acaagcttct
     1501 tcctgatacc gctgccaacg ccatgtcaag tgtggctgcc gcagattaca ccttaacctt
     1561 ccgtcccggc tatgagccgg aggaggaaag cgcagaagag tggtttaaaa agcttactgc
     1621 cttttttgac cagcctcagg ttatggtcct taaaaagacg aaaaagggcg aaaaggaaat
     1681 ggaccttaag cctcttatct atgaccttgg cgtgattgcc ggaaatgatg cagcacagtc
     1741 acagctcttt atgaagattt ccaccggaag cgcatccaat attaagccgg agcttttgtt
     1801 ggatgcttat tatgaagcac tgggcaagga acgttctccc tttgccttta tggttcagag
     1861 agaggaagta tacgcagatc aggcatcaga gcatgagaga gagcagggaa ttcattcttt
     1921 tattcctctt ggagctttgg gacaggacat tgagtgatgg aaaaacggat cattacatgg
     1981 cttaggggca ggatctgtac tgcttttatc caggacggct ctttggtgga gttgactctg
     2041 gaggacgagg attgctctgt tttaaaccgt atttatgtgg gacgggtaca gaaggtagta
     2101 ccgggcatca acgcggcatt tatcgacacg ggagacggtg taggctatta cagtcttaca
     2161 gaaaaccgca gccatctgtt taccgacagg aaccggaaag aggggccttt gcgccaggga
     2221 gatgaaatcc tggtgcagat cagccgggag ggcgtaaaga caaaggctcc ggtgctgaca
     2281 gcaaacctgt cccttcccgg ccgtctctgt gtggttaccg caggcagaac aggcattggc
     2341 ttttccaata agatcagcga cagcctgttt aaaaaggaag caaaggcctg tctggaggaa
     2401 gctcccttaa gtgggtttgg cgtgatcgta cggacgaatg cctaccaggc cggtatggat
     2461 gcgcttataa aagaatgtat ccttttaaaa gaacgtctgg aagccttaat aaaagacgcg
     2521 gcctgcagag tgagcggttc ctgcctgtac cagcctctgc cgtcctatat tacaggggtg
     2581 cgggatgcct atgaaggccg gctttcctct attgtaaccg atgacagggg gctgtatgac
     2641 cagcttaagg cctatctgaa ggaagagcag cctgaggatc tggaaaagct tgaattttat
     2701 gacgacagcc ttctgccttt atataagctt tacggcctgg aaactgtact ggatcgtgct
     2761 cttggaaaac gtgtctggct taaatccggc ggctatctgg ttattgagcc tacagaggct
     2821 atgacggtga tcgatgtaaa cactggaaag tatgaaggca gaaagaaaat ggccgacacc
     2881 atccgaaaaa tcaataacga ggcggctttt accattgcag cccagctgcg tttaaggaat
     2941 ctttcaggca tcattatggt agattttatt gatatggagc aggaggagga caaaaaggcc
     3001 cttttagact gcttaaaaca ggctgtgtca acggatccgg tccgtacggt ggttgtggat
     3061 atgacgcctc tggggctggt ggaactgaca agaaaaaagg taagaaaacc gctttatgaa
     3121 caggcgggac aaacctatga cgggatgcgg ggggataaaa acctatgaag atcataattg
     3181 ttggctgcgg taaggtggga accaccctgg ctgaacagct gaacagagaa aatcatgata
     3241 tcacactcat tgatacaaat gaggaagcca tacaaaacat ttccgacagc gcggatgtaa
     3301 tgggcgtgac gggaaatggt gcagtttatc aggtacagat ggaggcaggg atccaggatg
     3361 cagatcttct gatcgctacc acaaattcag atgaattaaa tatgctctgc tgtctgatcg
     3421 caaaaaaggc cggaaactgc cacaccatcg cccgtatccg aaatccggaa tattcatccg
     3481 agatccgtta tatcagagaa gagctgaatc tgtctctggc tatcaatccg gagctggcag
     3541 ccgccagaga aatcgcccgc cttcttcgtt tcccctctgc aattaagatc gagccttttg
     3601 caaaggggcg gatcgagctt ttaaaattcc tgatcccgga gcattctctg ctgaatgata
     3661 tgcgggtcat ggatgttgta aaccgtctaa aaagcaatgt tctgatctgt gtggtggagc
     3721 gcggaaatga cgttgtgata ccggacggta attttgtgat gaaaaaggga gataaaattt
     3781 cctttattgc ctctcatcag g
//
LOCUS       DBMDUH010000197         3779 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_8646_length_3779_cov_6.134584, whole genome shotgun sequence.
ACCESSION   DBMDUH010000197 DBMDUH010000000
VERSION     DBMDUH010000197.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3779)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8646_length_3779_cov_6.134584"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(102..1160)
                     /locus_tag="ACZPCG_12315"
     CDS             complement(102..1160)
                     /locus_tag="ACZPCG_12315"
                     /inference="COORDINATES: protein motif:HMM:NF012742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR1285864.1"
                     /translation="MKSKKITIKDVAKEAGVSVATVSRALNGLGGIGKETRDHVLEIC
                     ERMAYVPSTLASGLTMRHTRTLGIVVPDITSPFHAQLMVLLTSEAQQYGYQVLLCNSF
                     RDYETEMEYFKLLISNQVEGILFYPVGDMSSKNLYQYMRYVPIVSLNEMPDNSPIPYV
                     CGDERKGGSLAAERLIRGGCKKILCIGIKEDRLVHRYRVEGIMDEARQWGAEIEVYES
                     DKNYKSSFDRGYGQASELIRESLRPGGEPLPDGIIAASDATANGVIKACMEYGIAIPD
                     QMSVIGFDDINGAVPAVELTSVSISHKLHVKRAINLLIRMKRERTLKEQERGIKLRPA
                     LVERSSCQAVRGSGIYKQ"
     gene            complement(1593..2960)
                     /locus_tag="ACZPCG_12320"
     CDS             complement(1593..2960)
                     /locus_tag="ACZPCG_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008374125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1285865.1"
                     /translation="MEQDMTKGSPFRVLIMFTLPLAIGNIFQQLYNMADTIIVGRYVG
                     ADALAAVGSTGTVMFLLNGFAQGITAGFAVLTSQRYGAKKMDGVRQSVSNGILLSLIG
                     AIGFTAASLLFMRPLLHLMNTPENIFAQAYEYISLISLGMVANVFYNLFSSYLRAVGN
                     SRMPLVFLVLSACLNVVLDLILIINFKMGVAGAAWATNLSQAISAVLCAVYIYKKVPA
                     LMPEKRHWRFHKGDTRYQLATGIPMALQFAITSSGTMVMQSAINLFGSEAVAAYTAAG
                     KIHGLLTQGMVAMGQTMAVYSGQNYGKGDAGRIRQGVRAALIIEVIYSVASAVVVSAA
                     LGPSLGLFFSGEVDLAAMMPWARTYTTICTLFYVPLCTIFIFRNTMQGCGYGFLPMMG
                     GVVELAARLIVAVIAMDIHSYWLACACDPAAWVGAGLFTGISYLYVIKKIEKNLGKGT
                     AVHKS"
     gene            complement(3115..3738)
                     /locus_tag="ACZPCG_12325"
     CDS             complement(3115..3738)
                     /locus_tag="ACZPCG_12325"
                     /inference="COORDINATES: protein motif:HMM:NF016214.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HIR1285866.1"
                     /translation="MMGKTHMIAGIGAAVLISPPDTIGGCLQALLGGALGGLICDLDV
                     QSSTYCRDARVVRNTALLLGAVILFLDWKWSTGIWDHIGSHLGSRQLLGLGIFLVITI
                     AGKLSSHRSFTHSFLALGFLMAAFYFLYAPLIPYVAAGFLSHMFLDLLNKKPVEIFYP
                     YKKGVCLRLFSADGLANRLICTVSLVFVVVQMWDIMWKLVLGAAGRF"
BASE COUNT         1018 a         1022 c          756 g          983 t
ORIGIN      
        1 tacccaacgg atatcttcat ctgacagata gaatggtttc tgtttcagat caaccatata
       61 ctcgtacctc cttgtgatgt atgtctgcag ttccccaaaa actattgttt atatatgcca
      121 ctgcctctga ccgcctgaca ggaacttcgt tctaccaaag ctggtcgaag ttttatcccc
      181 cgttcctgtt ccttcagggt acgttcccgt ttcatacgga tcaaaagatt aattgcacgc
      241 ttcacatgaa gcttgtgtga aatcgacact gacgtcagtt ccactgcagg aaccgcaccg
      301 ttgatatcat caaaacctat cactgacatc tggtcaggaa tcgctattcc atattccata
      361 catgccttga ttactccgtt ggctgttgca tcactggccg caatgatgcc atccggaagc
      421 ggctctcctc ccggcctgag gctttcccgt atcagttcac ttgcctgtcc atatccacga
      481 tcaaaactgc ttttatagtt tttatcactc tcgtagacct caatctctgc tccccactgt
      541 ctagcctcat ccataatccc ttccacccgg taacgatgaa ccagacggtc ttccttgata
      601 ccaatgcaga gaattttttt acatcctccc cgaatcaggc gttcggctgc cagacttcca
      661 cccttgcgct catcaccgca cacataggga atcgggctgt tatccggcat ttcatttaat
      721 gaaacgatcg gaacgtaccg catatactga tacagatttt tggagctcat atctcccaca
      781 ggataaaaca ggatcccctc cacctgatta ctgatcagaa gcttgaaata ttccatctct
      841 gtttcataat cccgaaaact attgcagaga agcacctgat aaccatactg ctgtgcctcg
      901 ctggtaagaa gcaccatcag ctgtgcatga aacggactgg taatatccgg caccacaatc
      961 cccagcgttc ttgtatgacg catcgtcaat ccgctggcga gtgtactggg tacataggcc
     1021 atgcgttcgc aaatttccaa tacatgatct ctggtttcct tcccaattcc acccaagcca
     1081 ttaagcgctc tggatacagt tgcaactgaa acgcctgcct cttttgcaac atctttgatt
     1141 gtgatttttt ttgacttcat cggctgtctt tccactccgt tcgtaaacat ttccctcgtc
     1201 atatataaag tataacataa cctttcatcc gaaactatcc gactatttta cgatacgttt
     1261 tgcgtaactt tgtaagaagt gcacaaattt tatgcatatt ttttatcgct ttcgtcgaaa
     1321 ctatttttga atttccttgt gttttacatt tatttttatt agttttatct tttttatatg
     1381 tcttcaaaaa cagcagaagg tcattttgca catttcgcaa tgtgatgcac agcttattct
     1441 tccgaacgcg ccggcaaaag aactgcggcg ttatgttcaa ggtgaatata tctatgtccc
     1501 tctcaaactg gaaaacggat gcagcagcca tgtgatcaca gccgccgcgt ccgttttttg
     1561 caggtgcaga tattcttaaa gttatcttac ttttatgatt tatgaactgc cgtccccttc
     1621 cccagatttt tctcaatctt ctttattaca tacagatagg aaatgcctgt aaacagaccg
     1681 gctcccaccc aggcggcagg atcgcaggcg caggccagcc aatagctgtg aatatccata
     1741 gcgatcactg ctacgatcag acgggctgcc agctctacca ctccgcccat cataggcaga
     1801 aagccgtatc cgcagccctg catggtattt cggaagataa aaatagtaca taaaggcaca
     1861 taaaacaggg tacagatggt tgtatacgtt ctggcccagg gcatcatagc cgccagatct
     1921 acctcccctg agaagaacag acccagactt ggccccagag ctgccgatac gaccactgca
     1981 gatgccaccg aatagattac ctcaatgatc aaagccgcac ggacaccctg gcgaattcgt
     2041 cctgcatcac cttttccgta attctggccg ctgtatacag ccatcgtctg ccccatagct
     2101 accattccct gggtcagcag gccatggatt tttccggctg ccgtgtaggc ggccaccgcc
     2161 tcagagccaa acagattaat cgctgactgc atcaccatgg ttcccgagga ggtaatggca
     2221 aactgcagcg ccatgggaat ccccgtagcc agctgatagc gggtatcgcc cttgtggaat
     2281 cgccagtggc gcttttccgg catcagggcc ggcacctttt tatagatgta gactgcacat
     2341 aatacagcag aaattgcctg agacaggtta gtcgcccagg cagctccggc tactcccatt
     2401 ttaaagttga tgatcaggat cagatccagc actacgttta aacaggccga cagcaccaga
     2461 aaaacaagag gcatacggct gtttcccaca gctctcagat aggaagaaaa caggttataa
     2521 aatacattgg ccaccattcc caggctgata agggaaatat actcataagc ctgcgcaaaa
     2581 atgttttccg gcgtattcat cagatgcagc aagggacgca taaataaaag gctggctgcc
     2641 gtaaagccaa tggcgccgat cagagaaaga aggataccgt tcgatacgct ctgtcttacc
     2701 ccgtccatct tttttgcccc gtagcgctgg gaggtcagca cggcaaatcc ggctgtaatg
     2761 ccctgtgcaa agccgtttaa taaaaacatc acggttcccg ttgatcctac tgctgcaagc
     2821 gcatccgcac ccacatagcg ccccacaatg atggtatctg ccatgttgta aagctgctgg
     2881 aaaatatttc caattgcaag aggcagtgta aacatgatca gaacccgaaa agggcttcct
     2941 tttgtcatat cctgttccat tgcagatttc ctttccattc ttacagattt cagttatgac
     3001 attgtagcac aggtatgaat cattgtcaca catcaaatta aacgaaattt attttctaac
     3061 gcattaaaat ccgtaatctc atacaatcat accatccatg gataagccca gagtttaaaa
     3121 acggcctgcc gcgcccagaa ccagcttcca cataatatcc cacatctgaa ccacaacaaa
     3181 aacaaggctt acggtgcaga tcaacctgtt tgccagccca tccgcagaaa acagccgcag
     3241 gcaaacacct tttttatatg gataaaaaat ttctaccggc tttttattta acagatccaa
     3301 aaacatgtgg gaaagaaagc ctgctgccac ataggggatc agcggagcat acagaaagta
     3361 aaacgcagcc atcaaaaacc ccagtgccag gaaagaatgg gtaaagcttc tgtgggagga
     3421 cagctttcct gcaatggtta tcaccagaaa aatcccaagt ccaagaagct gcctgctccc
     3481 cagatgggaa ccgatatgat cccagattcc ggtgctccat ttccagtcta aaaataagat
     3541 cacagcccct aaaagcaggg ctgtatttct cacgacccgc gcatcccggc agtaagtgct
     3601 ggactgtacg tccagatcgc agatcagtcc acccagagct cctcctaaaa gagcctgaag
     3661 gcagccgccg atagtatcag ggggcgagat cagcacagcg gccccgatcc cggctatcat
     3721 atgtgttttt cccatcatgt cgtatcatct cccgtcttgt cttttgtcta aaaatggga
//
LOCUS       DBMDUH010000198         3771 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_8668_length_3771_cov_6.640872, whole genome shotgun sequence.
ACCESSION   DBMDUH010000198 DBMDUH010000000
VERSION     DBMDUH010000198.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3771)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8668_length_3771_cov_6.640872"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(12..1043)
                     /locus_tag="ACZPCG_12330"
     CDS             complement(12..1043)
                     /locus_tag="ACZPCG_12330"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008721617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIR1285867.1"
                     /translation="MGFTLVNELPTPAEILEQFPLADHLAALKKERDEEIKKVITGQS
                     NKFLVIIGPCSADNEDAVCDYANRLAKVQEATKDQLIIIPRVYTNKPRTTGEGYMGML
                     HQPDPEKKPDMLQGILAIRHMHMRVLQETGMSTADEMLYPDNLQYLSDIMSYIAVGAR
                     SVENQYHRLVASGCDVPVGMKNPTSGDMTVMLNSVVAAQLPHDFIFRGDEVSCPGNPL
                     THVILRGAVNKRGQCSPNYHYEDLRLLYDLYSQRELKNPACIVDTNHSNSNKQYKEQV
                     RIAKEVLHSRRYSNDIRHLVKGLMIESYIEPGSHKIGQHCYGQSITDPCLGWEESERL
                     IYEIADMVE"
     gene            1626..2852
                     /gene="tyrS"
                     /locus_tag="ACZPCG_12335"
     CDS             1626..2852
                     /gene="tyrS"
                     /locus_tag="ACZPCG_12335"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HIR1285868.1"
                     /translation="MQIYEELIARGLIAQVTNEEEIKKMVNEGKAVFYIGFDPTADSL
                     HVGHFMALCLMKRLQMAGNKPIALIGGGTGMVGDPSGRTDMRTMMTPEIIQHNCDCFK
                     KQMSRFIDFSEGKAMMVNNAEWLMDLNYIEFLREIGPHFSVNNMLRAECYKQRMEKGL
                     SFLEFNYMLMQSYDFYELFQRYGCNMQFGGDDQWSNMLGGTELIRRKLGKDAHAMTIT
                     LLLNSEGKKMGKTQSGAVWLDPNKTSPFDFYQYWRNVADADVLKCLRMLTFLPLEQID
                     EMDKWEGSQLNQAKEILAFELTKLVHGEEEAAKAQEGARALFSAGGSTINMPTSELTE
                     EDFKENAMDILTILTKSGLASSRSEARRNVEQGGVSVDGETVKDIHAAFEKDRFAGEG
                     LVVKRGKKNFRKVILK"
     gene            2878..3621
                     /locus_tag="ACZPCG_12340"
     CDS             2878..3621
                     /locus_tag="ACZPCG_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285869.1"
                     /translation="MKKAGILRCMKALAAAATLTIVGAFTALAGSAGKTPVPVTLHLD
                     VNNRQTAIYDGYTSYVMGNRVSDKDTFTITKDRNDVNLDEIVLNYYLITYEGDTQKSL
                     ECRVYGLEEGEHYPVVRPETLAKESESRLYDSLERCYVLEFVRGDERRSYYFMLLPEA
                     EMPSYRNIHLGSWEKDKKGWRYLYQENYLTSWALINDSWYFFGMDGYMKTGWQEYKEN
                     WYYLNPDNGVMMTNCTIDGYQVDGSGVRI"
BASE COUNT         1037 a          797 c          998 g          939 t
ORIGIN      
        1 ttttttattt attattcaac catatcggca atctcataga tcaggcgttc gctttcctcc
       61 cagccaagac agggatcggt gatggactgt ccgtagcagt gctgtccgat cttatggctg
      121 ccgggctcga tgtagctttc aatcatcagg ccctttacca gatggcggat atcgttggag
      181 tagcggcggc tgtgaagtac ctcttttgca atgcgcacct gctctttgta ctgtttgttg
      241 gagttggaat gattggtgtc cacgatgcag gccggatttt taagctctct ctgggaatag
      301 agatcataga gcagccttaa atcctcatag tgatagttgg ggctgcactg accgcgctta
      361 ttaactgcgc cgcgcaggat cacatgggtg agaggattgc cggggcagct tacctcatct
      421 cctcggaaga taaagtcatg gggaagctgg gctgcaacaa cagagtttaa cataacagtc
      481 atgtcgccgc tggtagggtt tttcatacca acaggtacgt cgcagccgct ggctaccagc
      541 cgatggtatt ggttttcaac cgagcgggcg ccgacggcaa tataggacat gatatcggac
      601 agatactgca ggttgtccgg ataaagcatc tcatcggctg tagacatacc ggtttcctgg
      661 agcacacgca tatgcatatg gcggatggca aggatcccct gaagcatatc cggctttttt
      721 tcgggatccg gttgatggag catacccata taaccttctc cagtggtccg cggcttattc
      781 gtatagactc tggggatgat gatcagctga tcctttgtag cctcctgaac ctttgcaagg
      841 cggttcgcat agtcgcagac cgcatcctca ttatcagcgg agcagggacc gatgatcacc
      901 aggaatttat tactctggcc ggtaatgacc tttttaattt cttcatctct ttcttttttt
      961 aaagctgcca gatgatccgc aagaggaaac tgttcaagaa tctctgcggg agttggcagt
     1021 tcatttacaa gtgtaaatcc catctcatat tcctcccttt gtgtgtaaat tcgataatgc
     1081 aacattataa tatacaaaaa cagattcgta aagcaaaagt gtactgtctt gacaaatcta
     1141 ttttacctgc ctataatagt gataatttca tgaaatggct atgacgggaa aaagtagtat
     1201 tctgcacaag catacagaaa gcagccgggg gatgagaggc gcatggaaag caggatatga
     1261 atacatctct gagcttcgca ccaaacggac ggaatctgca caggtttttc cggctcagta
     1321 ggctgcgacg gatccggcac acgttatcgt gcctgagtat ctgccggctg gaaagcggtt
     1381 tatttatttt acggcatgta cttaaggagg cagtcagtgc gagctggctg taaacatcag
     1441 gtggtaacac gggctttctg ctccgtcctt ttgaacaggg gacggagctt ttttagttcc
     1501 atagagaagc gaggcttatg aaataaaaac gcttcccagc ctgaagccgc cgaaactgta
     1561 agaaatcagg cctttgcatg aaaatagaaa tatggtaaaa taaaaacagt aaaggagaga
     1621 agacaatgca gatctacgaa gaactgattg ccagaggctt aattgcccag gtaacaaacg
     1681 aagaagaaat caaaaaaatg gtgaatgagg gaaaggccgt attctatatt ggatttgacc
     1741 cgaccgcaga cagccttcat gtaggccact ttatggctct ctgtctgatg aagcgccttc
     1801 agatggcagg aaataagcct attgccctga tcggaggcgg caccggtatg gtaggagatc
     1861 cttccggccg tacggatatg cgtactatga tgacaccaga gatcatccag cacaactgcg
     1921 actgctttaa aaagcagatg agccgtttca tcgacttttc cgaaggaaag gccatgatgg
     1981 taaacaatgc tgaatggctg atggatttaa attacattga gtttttacgg gagatcggtc
     2041 ctcatttctc tgtaaataat atgttaaggg ctgagtgcta taagcagcgg atggaaaagg
     2101 gcttaagctt ccttgagttc aactacatgc tcatgcagag ttatgacttc tatgagctgt
     2161 tccagcgcta cggctgcaat atgcagttcg gcggcgacga tcagtggagc aatatgctgg
     2221 gaggtacaga gcttatccgc cgcaagctgg gaaaagacgc ccacgccatg accattaccc
     2281 ttctgttaaa ctcagaggga aaaaagatgg ggaagaccca gtccggcgcc gtatggctgg
     2341 atccaaacaa gacttcccca ttcgatttct atcagtactg gagaaatgtg gcagacgccg
     2401 atgtgcttaa gtgcctgcgt atgcttacat tccttccgtt agagcagatt gacgagatgg
     2461 acaaatggga aggcagccag ttaaatcagg caaaggaaat cctggcattt gagcttacca
     2521 agctggtgca cggcgaggaa gaggctgcaa aggcacagga gggcgcaaga gcgctgtttt
     2581 cagctggagg aagcaccatc aatatgccta ccagcgagct gacggaagag gattttaaag
     2641 aaaatgccat ggatatcctg acgatcctga cgaaatcagg actggcttct tcacgttctg
     2701 aggcaagacg aaatgtagag cagggcggcg tatctgtaga cggagagact gtaaaggata
     2761 ttcacgctgc ctttgaaaag gaccgttttg ctggagaggg ccttgtagta aagagaggca
     2821 aaaagaattt cagaaaagtg atattaaagt aatgaagcag tgggaggagg tggccggatg
     2881 aaaaaggcag ggattctgcg ttgcatgaaa gcgctggctg cggcagcaac cttaaccatc
     2941 gtgggcgcct ttaccgccct ggcgggttct gccggaaaaa cgccggttcc ggtaacgctc
     3001 catctggatg taaataaccg gcagacggcc atctatgacg gctatacgtc ctatgtaatg
     3061 ggaaaccgcg tatcggataa ggataccttt accattacaa aggacagaaa tgatgtaaat
     3121 ctggatgaga ttgttttaaa ttattatctc atcacctacg agggagatac tcaaaagagc
     3181 ctggagtgcc gggtgtacgg attggaggag ggagagcatt atccggtcgt acgcccggaa
     3241 acactggcaa aggaaagcga aagccggctc tatgattctc tggagcggtg ctatgttctt
     3301 gaatttgtca gaggagatga gcgaagatcc tattatttta tgcttcttcc cgaggcagag
     3361 atgccttcct atcggaatat ccatctagga agctgggaaa aggataaaaa gggctggaga
     3421 tatctttatc aggaaaatta tcttacctcc tgggccttga tcaatgacag ctggtatttt
     3481 ttcggtatgg acggttatat gaaaaccggc tggcaggagt acaaagaaaa ttggtattat
     3541 ctgaatcctg ataatggcgt tatgatgacg aactgtacta tagatggata tcaggtggat
     3601 ggaagcggtg tgagaatctg accagttaca ttaaaaaagc atccgggcag tccgggaacg
     3661 gtatcgttct ggaatgtccg gatgcttttt taaatctctg ttttatccca gaaagcccag
     3721 atgctccaaa aggattttaa gccccatgag gatcaggatc aggccgccgg c
//
LOCUS       DBMDUH010000199         3737 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_8752_length_3737_cov_6.030253, whole genome shotgun sequence.
ACCESSION   DBMDUH010000199 DBMDUH010000000
VERSION     DBMDUH010000199.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3737)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8752_length_3737_cov_6.030253"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="ACZPCG_12345"
     CDS             <1..651
                     /locus_tag="ACZPCG_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HIR1285870.1"
                     /translation="REEKAFFMLNTSGYNIGNFTMPFAQSFLGPAGVITTSLFDTGNS
                     FICLGGAFSAAKMVKEGEGKISLIPIIKTLVRSVPFDAYITMTALSLLHISLPAPVIT
                     FSEVVSGANAFIAMLMMGVGFKISGDTSQKGKIVKILAVRYGVSIAVAAALYFFMPLP
                     LEYRQALAILPLSPIASAAPAFTADLKGDFGLASAVNSISIVISIVLITATLLIIL"
     gene            complement(775..1716)
                     /locus_tag="ACZPCG_12350"
     CDS             complement(775..1716)
                     /locus_tag="ACZPCG_12350"
                     /inference="COORDINATES: protein motif:HMM:NF014376.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1285871.1"
                     /translation="MKLSEMQKYKELVSAHKRSEISNEELQKTFITDGAILSNLYQEM
                     EMDSDSVDCHDDVNYTKDSIQLHSHTFYEMLYCRSGNVQYLLGAERYRVRRGDIIFIP
                     PGISHRPLYLDQLAEPYSRYVIWIHPEFMKNIQQFLGFIPLSDPVLLRTQDSGWEYME
                     RYFQSACREAKIRQEGYEACISSITVQLLIHLTRACTHSKATEPLTEKKELLDELIRY
                     IEDHLAEKITLKSAATDFYISERAISRLFSEKMDVSFYRYVTQRRLIAAKTRIINGDS
                     LETIGEQVGFSDYSTFYRAFKQAFSISPQQFKKLQKA"
     gene            1886..3265
                     /locus_tag="ACZPCG_12355"
     CDS             1886..3265
                     /locus_tag="ACZPCG_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006774766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1285872.1"
                     /translation="MNDKSLRPFGVKDQIGYVFGDMAGSFVNLFVDAYFLTFCTYVLG
                     ISPGWMAGLFLAARLWDAINDPIMGSFPDRWRLGKSGDKFKPWIKLFMIPLALSGILC
                     FANVPFEGIALHAWVAFAYVFYGMSYTGTSMPFGAMASVVSTDPIDRSKLSRARSIGG
                     TVVGIGALSVVPILCFDKDSNIIPERFGLIAVIFGILCVISYLLLCNLTIERVRQPKQ
                     KGEKFNYGNVLKAAVKNRPLIGVMVATIGSMLYITGSGQLKSYIYKEFYQDTSLMTLA
                     SIGTLPVMIICFILVPKIVQKFGKRNVILVMSTYNLLLSTYILLFPIQSALLYTILNL
                     LATIGQTAFTILIWALVTDCLDYSEWKMNFRADGSMYSLYTFSRKIGSTIASTGAASA
                     LAAIGYVSGVGAVQSAATINNIYYLVNAVPVITCILELIGIGLIFNLNQKKTAEMYAA
                     LAERRNSQN"
     gene            3310..>3737
                     /locus_tag="ACZPCG_12360"
     CDS             3310..>3737
                     /locus_tag="ACZPCG_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR1285873.1"
                     /translation="MKKIDLHLHLSFDPVPKSDTLLVSTYKEMLPHLEELGIGKGVLM
                     SSGERNFSMPMGSNEENCRIAAADPEHYAWMCNLDYDGDPKTVYDRLSVCKEKGAVGI
                     GELMINRRLDDPFLNAVFEAAGKLNLPVTFHMSPETGYSYG"
BASE COUNT          951 a          846 c          851 g         1089 t
ORIGIN      
        1 cgtgaggaaa aagcattttt catgttgaac acctcgggct ataatatagg aaacttcact
       61 atgccctttg cccagagctt tctagggcca gccggggtaa tcacaaccag cctttttgat
      121 acgggaaatt cctttatctg tctgggcgga gcctttagtg cggcaaagat ggtaaaggag
      181 ggagagggaa agatatccct gatccctatt ataaaaaccc tggtgcgttc cgtacccttt
      241 gacgcttata taaccatgac ggcgctgtcc ctgctccaca tcagccttcc ggctccggtg
      301 atcacatttt cagaggtggt ttcaggggcg aatgccttta tcgccatgct gatgatggga
      361 gtaggcttta aaatttccgg agataccagc cagaagggaa aaatcgtaaa gatcctggca
      421 gtgcgttacg gcgtctcgat cgcagtggcg gcggccctct atttttttat gcctcttcct
      481 ctggaatacc gtcaggctct ggcaatcctg cccttgagcc ctattgcgtc tgcagcgccg
      541 gcctttacgg cagatttaaa gggcgacttt ggacttgcca gtgcagtcaa ttccatttcc
      601 attgtaatca gtattgtact gatcacggca actctcctga taattctttg atacggagtg
      661 tataactttg acagaagcgg ggataatgag attatcacaa aagagaaaat aaatacttat
      721 cctccccttt ttaatacctc gtttattggc cttgtgcagt agacggggta ttttttatgc
      781 cttctgcaat ttcttaaact gctggggcga tatagaaaag gcctgtttaa acgccctgta
      841 aaaggtggag tagtcggaaa accctacctg ttctcctatg gtttccaggg aatctccatt
      901 gataatcctg gtttttgcgg caatcagccg gcgctgggtt acgtaccggt aaaagctgac
      961 gtccattttt tcgctgaaca ggcgcgatat ggcccgttca ctgatgtaaa aatcagtagc
     1021 cgctgatttt aaggttattt tttcagccag gtggtcttct atatagcgga tcagctcatc
     1081 cagcagctct tttttctcag ttagcggttc cgtagccttg ctgtgggtac acgccctggt
     1141 caggtggatt aaaagctgca ccgttatgga agaaatacag gcttcatatc cttcctgtct
     1201 gattttagcc tccctgcagg cagattgaaa atatctttcc atgtactccc acccgctgtc
     1261 ctgggttctt aacagaaccg gatccgataa tgggataaaa cctaaaaact gctggatatt
     1321 cttcataaat tccgggtgga tccatatcac atagcgggaa tagggttctg caagctgatc
     1381 taagtacagg ggcctgtggc tgatcccggg cggtatgaaa ataatatctc ccctgcggac
     1441 ccgataccgt tctgctccca gaagatattg tacatttccg ctgcggcaat ataacatctc
     1501 ataaaacgta tgactgtgaa gctggatgct gtctttcgta taattcacat catcatggca
     1561 gtctacagaa tctgaatcca tttccatttc ttgataaaga ttggataaaa ttgccccgtc
     1621 ggtaataaac gttttctgaa gttcttcatt gctgatttcc gatctcttat gcgcagaaac
     1681 cagttcttta tatttttgca tttctgataa tttcattgct tttcctgttc cttcctcctt
     1741 cttatcataa tctaatttgg cttaatttgc aatatattat gcgatatttg caatagtaat
     1801 tgcaatagca gtatagtatt cttaaaacac aataagttaa tggtctgcgg ttatctgccg
     1861 cagcaaagac aaggaggaac atgatatgaa tgacaaatct ttacgcccct ttggagtaaa
     1921 ggatcagatc ggttatgtct tcggggatat ggccggaagc tttgtaaact tgttcgtaga
     1981 tgcctatttt cttacattct gtacctatgt tctgggaatt tcgccaggat ggatggcggg
     2041 actgttttta gccgcccgac tctgggacgc catcaacgac ccgatcatgg gttctttccc
     2101 agaccgctgg agacttggca aaagcggcga caagttcaaa ccatggataa aactgtttat
     2161 gattcctctg gccctttccg gtattttatg ttttgccaac gttccatttg aaggaattgc
     2221 ccttcatgca tgggtagcgt ttgcttatgt attctatgga atgtcctata ccggaaccag
     2281 tatgccattc ggcgctatgg caagcgttgt ttctacggat cccattgacc gttccaagct
     2341 ttccagagcc agaagtatcg gcggtacggt agtcgggatc ggcgcgcttt ccgttgttcc
     2401 gattttatgt tttgacaagg attccaatat tattccggag cgttttggcc tgatcgcagt
     2461 aatattcggc attctgtgtg ttatttctta ccttctgtta tgtaacctga ccatagagcg
     2521 tgtccgccag ccaaaacaga aaggggaaaa attcaactat ggaaatgtat taaaagcagc
     2581 tgtcaagaac cgtccgttaa tcggcgttat ggttgcaacc atcggaagca tgctttacat
     2641 tactggaagc ggacagttaa aaagttatat ttacaaagaa ttctatcagg ataccagcct
     2701 aatgaccctg gcaagtatcg gaacccttcc ggttatgatt atctgcttta tcctggtccc
     2761 taagattgtg cagaaatttg gaaagagaaa tgtaatcctg gttatgagta cctataacct
     2821 gctgctttcc acttatattt tactgttccc gattcagagc gcgctgcttt acacaatttt
     2881 aaacctgtta gctacgatcg gacagaccgc ttttaccatt ttgatctggg ctctggttac
     2941 cgactgtctg gactacagcg aatggaaaat gaatttccgc gcagacggaa gcatgtacag
     3001 cctctatacg ttcagccgta agattggttc taccatcgct tctaccggcg ctgcatccgc
     3061 actggctgcg atcggctacg tttccggcgt aggcgctgta cagagcgcgg caacgatcaa
     3121 taacatttac tacctggtaa atgcagttcc ggttattacc tgtatcttag aactcatcgg
     3181 aatcggtctg atcttcaacc tgaatcagaa aaagaccgct gaaatgtatg cagccctggc
     3241 ggaacgccgg aattcccaga actgatcaag aaccatttgt gcgtacccga aaccggagga
     3301 ataaaatcta tgaaaaaaat tgatctgcat ctgcatttaa gctttgatcc tgttcccaaa
     3361 agcgatactc ttctagtaag tacctacaaa gaaatgctgc ctcatttaga agaactgggg
     3421 atcggaaaag gcgtgctcat gtccagcgga gaacggaatt tctccatgcc tatggggtcc
     3481 aatgaagaaa actgccggat tgctgcagcg gatccggagc attatgcctg gatgtgcaat
     3541 ctggattatg acggagatcc caaaactgtt tatgaccggc tgtctgtctg caaagaaaaa
     3601 ggcgctgtcg gcatcgggga actgatgata aaccgaaggc tggatgatcc ctttttaaac
     3661 gccgtatttg aagcggctgg aaaattaaat cttccggtta cttttcatat gagtccggaa
     3721 actggctaca gctacgg
//
LOCUS       DBMDUH010000200         3710 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_8824_length_3710_cov_7.478526, whole genome shotgun sequence.
ACCESSION   DBMDUH010000200 DBMDUH010000000
VERSION     DBMDUH010000200.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8824_length_3710_cov_7.478526"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="ACZPCG_12365"
     CDS             <1..306
                     /locus_tag="ACZPCG_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR1285874.1"
                     /translation="AKAISRAPGIGPKTAQRLILDLKDKVDLEEVISGFGGGNGSAVR
                     TEELSGLASAAKEAVEALTALGYTGAEASRAVKRVEMTEGMNSEDVLKASLKFLTFL"
     gene            350..1345
                     /gene="ruvB"
                     /locus_tag="ACZPCG_12370"
     CDS             350..1345
                     /gene="ruvB"
                     /locus_tag="ACZPCG_12370"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HIR1285875.1"
                     /translation="MERRIITTEATEEDVRIETTLRPQCLEDYIGQEKIKSTLKIYIE
                     AAKSRGEALDHVLFYGPPGLGKTTLSGIIANEMGTKMKVTSGPAIEKPGEMAAILNNL
                     QEGDVLFVDEIHRLNRQVEEVLYPAMEDFAIDIMLGKDSSARSIRLELPHFTLVGATT
                     RAGLLTAPLRDRFGVVQRLEFYTPQELKTIIIHSAGVLGVEIDPEGALELARRSRGTP
                     RLANRLLKRVRDFAQVKYDGAITKEVTDFALDIMDVDKLGLDQNDRNILLMIIEKFSG
                     GPVGLDTLAAALGEDAGTLEDVYEPYLLMNGLINRTPRGRMATESAYRHLGLEMG"
     gene            1413..2171
                     /locus_tag="ACZPCG_12375"
     CDS             1413..2171
                     /locus_tag="ACZPCG_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HIR1285876.1"
                     /translation="METKNYTQVLIDGKIYTLGGSEDEAYLQRTASYVNEKNMQLRGI
                     PGFSKQSADYQQVMIQLNIADDYFKALDHSEQMEEERNRMEKESYSLKHELVSTQMKL
                     EAVLKDLEERQVQLEETASHLAKLERENRNLTRQNERLSQERAEAVTEAEQMKALMLH
                     QENELRAVGEAAASEEEPVVEENPVLEEEPEVGKETGEETAEEEAAEVSNEEAEVSEP
                     AAAPEEEVRPVSDEELARRALQAARKAGFHRGRR"
     gene            2305..>3710
                     /locus_tag="ACZPCG_12380"
     CDS             2305..>3710
                     /locus_tag="ACZPCG_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002603760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HIR1285877.1"
                     /translation="MKEKKRAELLAPAGSFASLKAAVAAGADAVYMGGARFGARAYAQ
                     NADQDEMIAAIEYAHLHGCRLYMTVNTLFKENELGELYEYLLPYYKAGLDGVIVQDLG
                     ALSFIREHFPGIELHASTQMTITSVYGAKELKRLGCCRVVPAREVSLEEIRRIYDETG
                     MDIETFVHGALCYCYSGQCLMSSLIGGRSGNRGRCAQPCRLPYRVYEQENGTTVNKED
                     QKCVLSMKDLCTLDILPQILEAGVFSLKIEGRMKSPRYTAGVVRIYRKYLDRYLEYGS
                     EGYYVEPEDKKELLDLFDRGGFTSGYYTRHNGRDMIALKEKPEFRETNKELFDFLDRE
                     YVETEKKEPVEGYAYLAEGLPSVLTLTCGDISVTVSGQEPQAAKNQPMTREKVIRQLG
                     KTGATAFEFTELEAEVCGALFLPIQALNELRREGFEALTEAIHNQWRRKAPEAGEVQN
                     GADSGEKSSRAAGCAGPVP"
BASE COUNT         1007 a          761 c         1156 g          786 t
ORIGIN      
        1 gccaaggcca tttccagggc acccggtatc ggccccaaaa cggcccagag gctgattttg
       61 gatttaaagg ataaggtgga tctggaagag gtgatttccg gatttggggg cggaaacggc
      121 agtgcagtcc ggaccgagga actttccggg ctggcatcag ctgcaaaaga agctgtggag
      181 gctcttacgg cccttggcta tacgggggca gaggcttcca gggcggtaaa gcgtgtggaa
      241 atgacagagg gaatgaattc agaggatgtg cttaaggctt ctttaaaatt tctgaccttt
      301 ctgtaaggga agaacttaaa ggaaataatt ggaaaatgat gagggactta tggaacgcag
      361 gatcataaca acagaggcaa cagaggagga tgtgcgtatt gagactacgc tgcgtcctca
      421 gtgccttgag gactatattg ggcaggagaa gattaaatcc actctgaaaa tctatattga
      481 ggcagccaaa tccagagggg aagcgctgga tcatgtgctg ttttacggcc ctcctggcct
      541 tggaaagacc accctttccg ggattattgc aaatgagatg ggaacgaaga tgaaggtaac
      601 cagcggcccg gccattgaaa agcctgggga gatggctgcg atcttaaata accttcagga
      661 gggggatgtg ctctttgtgg atgagatcca ccgtttaaac cgtcaggtgg aggaggtact
      721 ttatccggct atggaggact ttgccataga tattatgctg ggaaaggatt cttcggcccg
      781 ctccatccgt ctggagctgc cccattttac tctggtgggc gctactacca gagccggcct
      841 tcttacggct cctttaagag acaggttcgg agtggttcag cgtctggaat tttatacgcc
      901 ccaggagctt aagaccatta tcatacattc cgcaggggtg ctgggagtgg agatcgatcc
      961 ggaaggggca ttagagcttg ccagacggtc cagaggtacg cccagactgg caaaccgcct
     1021 tttaaaaagg gtaagagact tcgctcaggt aaaatatgac ggagcgatca cgaaagaagt
     1081 gacggatttt gctctggata tcatggatgt ggacaagctg ggactggatc agaatgacag
     1141 aaatatcctg cttatgatca ttgagaagtt ttccggcggc cctgtagggc ttgatacgct
     1201 ggctgcggct ctgggggaag acgccggaac cttagaggat gtgtacgaac cctatcttct
     1261 gatgaacggc ctgatcaacc gcacgcccag aggaaggatg gccacagaga gcgcttatcg
     1321 tcatttgggc ttggaaatgg gatgaaaatg tggtaagctt ctgttagagg gaaagaacgt
     1381 ttccccggat cagacaggag aatgagtgaa gtatggaaac aaagaattat acacaggttt
     1441 taattgacgg aaaaatatat actctgggcg gaagcgagga cgaggcctat cttcagcgga
     1501 cggccagcta tgtaaatgaa aagaatatgc agctgcgtgg gattccgggt ttctccaagc
     1561 agagtgcgga ttatcagcag gttatgatcc agttaaatat tgcagacgat tattttaagg
     1621 cgctggatca ttcagaacag atggaagagg aacgcaaccg gatggaaaag gagagctaca
     1681 gcttaaagca tgagctggta agcactcaga tgaagctgga agctgtgctt aaggatctgg
     1741 aggagcgcca ggtgcagctg gaggagacgg cttcacattt agcgaagctg gaacgggaaa
     1801 acagaaatct tacccgccag aatgaaaggc tgtctcagga gcgggctgag gcagtgacag
     1861 aggcggagca gatgaaagcc cttatgcttc atcaggagaa cgaactccgg gcagtgggag
     1921 aagcggctgc ttctgaggaa gagcctgttg ttgaggagaa tcctgttttg gaggaagaac
     1981 ctgaggttgg gaaagagaca ggagaagaga ccgcagaaga ggaagcggca gaggtttcga
     2041 atgaagaagc ggaggtttct gaaccggcgg ctgctcctga agaagaggtt cggcctgttt
     2101 ccgatgaaga gctggcaaga agagcgcttc aggcagccag aaaggcaggc tttcacagag
     2161 gccgcagata gaaggataca aaaaagacag catcgggggg atgtctcatg gattcgtatg
     2221 gggcatcctt tcgttatccc tgaagagaac gggggagaaa tgtggttttg gatccgttaa
     2281 aagaccagga aaggcggaaa taacatgaaa gagaaaaaaa gagcagagct gcttgctccg
     2341 gcaggctcct ttgcaagctt aaaggcagca gtggcagcgg gcgctgacgc tgtatatatg
     2401 ggaggagcgc ggtttggagc cagagcctat gctcaaaatg cagatcagga tgagatgatc
     2461 gcagccattg aatacgccca tctccatggc tgccgccttt atatgaccgt aaatacgctc
     2521 tttaaggaga atgagctggg agaattatat gagtatctgc ttccctatta taaagccgga
     2581 ctggacggtg tgatcgttca ggacctggga gccctgtcct ttattcggga gcattttccg
     2641 ggaatagaac ttcacgccag cacccagatg accattacca gtgtttacgg ggcaaaggag
     2701 ctgaagcgtc tgggctgctg ccgggtagtg ccggccagag aggtatcctt agaggagatc
     2761 cgccggatct atgatgagac gggaatggat attgaaacct ttgtccacgg ggcgctgtgc
     2821 tactgctatt ccggacagtg ccttatgagc agcctgatcg ggggcagaag cggaaacagg
     2881 ggccggtgcg cccagccctg ccgtcttccc taccgggtat atgagcagga aaacggaaca
     2941 actgtcaata aagaggatca gaaatgtgtc ctcagcatga aggatctgtg cacgctggat
     3001 attttgcccc agattctgga agcaggtgta ttttcactga agattgaagg aaggatgaag
     3061 agccccagat atacggccgg ggtagtgcgg atttaccgga agtatctgga ccggtatctg
     3121 gaatacggtt cagaagggta ttatgtggag ccggaggata agaaagagct tctggatctg
     3181 tttgaccggg gaggctttac ctccggctac tacaccagac ataatggccg cgacatgatc
     3241 gctttaaagg aaaagccgga atttcgggaa acgaacaaag aactctttga ctttctggac
     3301 cgggaatatg tggaaacaga gaagaaagag cctgtggagg ggtatgcata tctggcggag
     3361 ggacttccgt ccgtgcttac gctgacctgc ggggatattt ctgtgacggt ttccggacag
     3421 gagcctcagg ctgcgaaaaa ccagccaatg accagagaaa aggtgatccg ccagctgggg
     3481 aaaacagggg ctacggcttt tgaatttaca gagcttgagg cagaggtatg cggagcgctg
     3541 tttcttccca ttcaggcatt aaatgaactg cgcagagagg gctttgaggc gcttacagag
     3601 gcgatccaca atcagtggag aagaaaggcg ccggaggcgg gagaagttca gaacggtgca
     3661 gattccggtg agaaaagcag ccgggcggct ggatgcgccg ggcctgtccc
//
LOCUS       DBMDUH010000201         3706 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_8837_length_3706_cov_5.473509, whole genome shotgun sequence.
ACCESSION   DBMDUH010000201 DBMDUH010000000
VERSION     DBMDUH010000201.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3706)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3706)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3706
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8837_length_3706_cov_5.473509"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            311..1720
                     /locus_tag="ACZPCG_12385"
     CDS             311..1720
                     /locus_tag="ACZPCG_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008722785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HIR1285878.1"
                     /translation="MKFSQDLQETMKYLNNELHTDRNFDVVYRVLTIGGKNACLYFID
                     GFTKDDTLLKLLQALSSIQADRMPKDAHSFSKQYLPYGEIGLIHESSEMVLQLLSGVS
                     CLFIDGYDACLTIDCRTYPARGVSEPEKDKVMRGSRDGFVETLVFNTALIRRRIRDPK
                     LSIEIMTAGESSHTDIAICYMENRVDQKLLSTIKKRIRDLEVDALTMNQESLAECLFP
                     YKWFNPFPKFKFSERPDSSAASLLEGNIVILVDTSPSAMILPSSVFDIIEEADDYYFP
                     PITGTYLRLTRMTISALSLFLTPTWLMFMQNTELIPDWLAFIRLSDPYNVPLIWQLLI
                     LEFAIDGLRLAAVNTPSMLTTPLSVIAGIVLGEYAVKSGWFNSETMLYMAFVTVANYS
                     QASFELGYAMKFMRVIILILTALFNLWGYAAGIVFTICAIVFNRTIAGKSYIYPLIPF
                     HWSELRKRFFRGRLPHREK"
     gene            complement(1727..2059)
                     /locus_tag="ACZPCG_12390"
     CDS             complement(1727..2059)
                     /locus_tag="ACZPCG_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR1285879.1"
                     /translation="MLDERWVFHENAKAEPAGEGVVRRVLAYSKDLMCVENTFETGAE
                     GKLHHHPHTQITYVVSGVFEFNIDGEIKTVKAGDTMLKLNGVEHGCVCKERGILLDIF
                     NPMREDFV"
     gene            complement(2090..3610)
                     /gene="galT"
                     /locus_tag="ACZPCG_12395"
     CDS             complement(2090..3610)
                     /gene="galT"
                     /locus_tag="ACZPCG_12395"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002603910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HIR1285880.1"
                     /translation="MISKAVKDLVSYGMKTGLLKEEDAVYARNQILDVLRINEYEEPK
                     AEGEEPELEEILKTLLDYAAEKGLLGGNSVVYKDLFDTKLMNCLMPRPSEVTAEFWKH
                     YEKSPQAATDYYYKLSQDSDYIRRYRIAKDMKWTAQTRYGTLDITINLSKPEKDPKAI
                     AAARKAKQSGYPKCQLCMENVGYAGRADHPARNNHRVIPIRVNDSQWGFQYSPYVYYN
                     EHCIVFNGEHVPMKIEKATFKKLFDFIRQFPHYFLGSNADLPIVGGSILSHDHFQGGH
                     YTFAMAKAPIERHFTMEGYEDVEAGIVFWPMSVLRLRCMDSDRLIELGDHVLKMWRSY
                     TDEAAFVFAEAEGEPHNTITPIARKNGNMYELDLVLRNNITTEEFPLGVYHPHQELHH
                     IKKENIGLIEVMGLAVLPSRLKSELAELGEYMAEGKDIRSNDAISKHADWVEEFLPKY
                     ESRGIRITADNAEEILKEEVGLVFARVLEDAGVYKCTEEGREAFGRFLKAAGFKEA"
BASE COUNT          902 a          991 c          757 g         1056 t
ORIGIN      
        1 cagaaggcag ctcttagtca cgatcaaaaa ccgtcacact ataaaatatt gctggcatcc
       61 ccataccaaa caaaaacccg taaggcacat ctctgtactt cttacgaggg atactatacc
      121 acctgtgtca atttctgtca actatttttg tgcaatttaa catatgcttt tccataatac
      181 ggaaattcta tatccatttc aacaatttta tttttctgtt ccccaaaaca gtattgacat
      241 atttttcaga aatcaggtca tactattgcc tgtcttactg ctatatgcaa agacagaaag
      301 gatccttatt atgaaatttt ctcaggattt acaggaaaca atgaaatatc tgaacaacga
      361 gcttcatact gatcgaaact ttgatgtggt gtaccgcgtc ctcactatcg gaggaaaaaa
      421 tgcctgtctc tattttatcg acggctttac aaaggatgat acgcttttaa agctcctcca
      481 ggcgctttcc tccatccagg cagaccggat gccaaaggat gcacacagct tttccaagca
      541 gtaccttccc tatggagaga taggcctgat ccatgaaagc agcgaaatgg tcctccagct
      601 tctctctggc gtatcctgtc tctttatcga cggctatgac gcctgtctta ccatcgactg
      661 ccgcacctat ccggcaagag gggtcagtga accggaaaag gataaggtaa tgcggggttc
      721 cagagacgga tttgtggaaa cccttgtatt taatacggcg ctgatccgcc gaaggatccg
      781 cgatccaaag ctttccattg aaatcatgac agccggtgaa agctcccata cggatatcgc
      841 catctgctat atggaaaacc gggtggatca aaagctgctt tccaccataa aaaaaagaat
      901 ccgggatctg gaggtcgatg ctctgaccat gaaccaggaa agtctggcag aatgcctctt
      961 tccctataag tggttcaatc catttcccaa attcaaattt tccgagcggc ccgattcctc
     1021 tgcagcctct cttttagagg ggaatatcgt aattctggtg gatacctctc cctccgccat
     1081 gattcttcca tcctctgtct ttgatatcat cgaggaggcg gacgactact attttcctcc
     1141 cattaccgga acgtacctca ggcttacccg gatgaccatt tccgcgttat ctctttttct
     1201 tacgcctacc tggctgatgt ttatgcagaa tacggagctg attcccgact ggctggcctt
     1261 tatccgtctg tccgatccct acaacgtgcc tttaatctgg cagcttctga tcctggaatt
     1321 tgccattgac ggcctgcggc tggcagctgt caataccccc agcatgctga ccaccccgct
     1381 cagtgtgatc gccggtatcg tgctgggcga atacgccgta aaatcggggt ggtttaacag
     1441 tgaaaccatg ctgtatatgg cctttgtaac cgttgccaat tattcccagg ccagctttga
     1501 attaggatac gccatgaagt tcatgcgtgt gatcatcctg atcctcacag ccctgtttaa
     1561 cctctgggga tatgcggcag gcattgtatt taccatctgc gccatcgtat ttaacagaac
     1621 catcgcggga aagagctata tttatcctct gatccccttc cactggagtg agctgcgcaa
     1681 acgtttcttc cgcggacgcc tgccccacag ggaaaaataa acgtttttat acgaaatctt
     1741 cccgcatggg attaaagata tctaaaagga tgcctctctc tttgcagaca cagccgtgct
     1801 ccacgccgtt taactttaac atggtatctc cggcttttac cgtctttatc tcgccgtcaa
     1861 tattaaactc aaacacaccg cttacaacat aggtgatctg agtatggggg tggtggtgaa
     1921 gttttccctc tgcccctgtc tcaaaggtgt tttccacaca cataagatcc ttgctataag
     1981 ccagcactct gcgcactacg ccttctccgg caggctctgc ctttgcattt tcatgaaata
     2041 cccatctttc atccaacatc tgtaaatcct cctgattctt tatgattatt tatgcttctt
     2101 taaagcctgc ggcctttaag aaacggccaa aagcttctct gccctcttca gtgcatttgt
     2161 atacgcccgc atcctccaga actctggcaa atacaagacc cacctcctcc ttgaggatct
     2221 cctctgcatt atcagccgtg atccggattc cccggctctc atacttggga agaaattcct
     2281 ctacccagtc tgcatgcttg cttatggcat cgttagaacg gatatccttt ccctctgcca
     2341 tgtattctcc cagctctgcc agctcgctct ttaatcggga aggcagcact gccagaccca
     2401 taacctcgat caggccgata ttttcctttt taatatggtg cagctcctga tggggatgat
     2461 atacgcccag aggaaattcc tctgtggtaa tgttattgcg cagcaccaga tccagctcat
     2521 acatattccc gtttttgcgg gcaatgggcg taatggtatt gtgaggctct ccctctgctt
     2581 ctgcgaatac aaaggccgcc tcatcggtat agctgcgcca catcttcagc acatggtcac
     2641 ccaactcaat gagcctgtcg ctgtccatac accgcagacg cagcacagac atgggccaga
     2701 atacgattcc cgcctctaca tcctcatagc cttccatggt gaaatgccgc tcaatgggcg
     2761 cctttgccat ggcaaaagta tagtgaccgc cctgaaaatg gtcatggctt aaaatagagc
     2821 cgcccacaat gggaagatcc gcgttggaac ccagaaagta atgggggaac tggcggataa
     2881 aatcaaacag cttcttaaag gtggcttttt caatcttcat gggaacgtgc tcgccattaa
     2941 acacgatgca gtgttcattg taatacacat aaggagaata ctggaagccc cactggctgt
     3001 cattgacccg gatcgggatc acacggtggt tattgcgggc cggatggtca gcccgtcctg
     3061 catatcccac attttccatg cacagctggc atttgggata accgctctgt tttgcctttc
     3121 ttgctgctgc aatggccttt ggatcctttt caggctttga cagattgatc gtaatatcaa
     3181 gagtgccgta gcgtgtttgt gccgtccatt tcatatcctt tgcgatgcgg tatctgcgga
     3241 tataatcgga atcctgactc agcttataat agtaatccgt agcagcctgg ggagattttt
     3301 catagtgttt ccagaactct gccgtcactt cgctgggtct tggcatcaga cagttcataa
     3361 gctttgtatc aaaaagatct ttgtatacaa cgctgttccc tcctaaaagc cccttttctg
     3421 ccgcataatc caaaagagtt tttaaaattt cctccagctc cggttcttct ccctctgcct
     3481 tcggctcttc atattcattt atcctcagca catccaggat ctggtttctg gcataaaccg
     3541 catcctcctc cttcagaagt cccgttttca tcccatagct taccagatct ttgactgctt
     3601 tactgatcat gtctgtaacc tccctgtgat tttttattga aaagacggct tttttaacca
     3661 gaaaaccgtc ctgactttat acctctatta taaaaggagc gcttcg
//
LOCUS       DBMDUH010000202         3686 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_8897_length_3686_cov_5.478940, whole genome shotgun sequence.
ACCESSION   DBMDUH010000202 DBMDUH010000000
VERSION     DBMDUH010000202.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3686)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3686)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3686
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8897_length_3686_cov_5.478940"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="ACZPCG_12400"
     CDS             <1..312
                     /locus_tag="ACZPCG_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007714798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HIR1285881.1"
                     /translation="TGIDTAMMAGYGDYRFLSIFLGVLTGVGGGVLRDIMAGQTPYIL
                     KKHVYACASLAGALFYVYLSDFLGESISMVAAAFLVVLIRLLATRYCWDLPKATTDEI
                     G"
     gene            complement(420..2090)
                     /locus_tag="ACZPCG_12405"
     CDS             complement(420..2090)
                     /locus_tag="ACZPCG_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HIR1285882.1"
                     /translation="MGFELLLGLLGGLALFLYGMQMMSTNLEAAAGNKMKQILERLTS
                     NRFLGVLVGAVITALIQSSSATTVMVVGFVNSGLMTLNQAVWVIMGANIGTTITGQLI
                     ALDVGALAPLIAFVGVAILVFVKNKKIQHIAGIVAGIGVLFIGMDMMSSAMSPLREEP
                     QFVEMMTKFSNPLLGILVGAGFTALIQSSSASVGILQALAMSGVIDLHSAVFVLFGQN
                     IGTCITAVLASIGTTRNAKRATAIHLLFNLIGTVVFVVLSLVTPFTDVMISLAPGNPM
                     AQIANVHTVFNLGTSLLLLPFGSVLVKIVERILPDTEVKVMDEDHWLQGLMASKHHLG
                     VSTIAISQIHEEIRDMLSAASKNVDASFLAVEQGPGEDGIGEIENREEEIDLANVRLS
                     KKISRVLVLDQTPKDIETLNKMYSILGNIERIGDHAMNLAEYAQTILEKDLSFSEYAQ
                     SEFSVMADSCSKGMKLLEQAAAGEKKFELAKVQEIEQKIDDITRKFRQNQIDRMRAGS
                     CNVEASILYSEMLTDYERIGDHMLNIAEAYDVINKNDEGISDTQPANA"
     gene            complement(2404..3552)
                     /gene="fucO"
                     /locus_tag="ACZPCG_12410"
     CDS             complement(2404..3552)
                     /gene="fucO"
                     /locus_tag="ACZPCG_12410"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HIR1285883.1"
                     /translation="MANRIVLNETSYHGAGAIAEIANEAKARAFKKAFVCSDPDLIRF
                     NVTKKVTDVLDEAGLSYEIYSNIKPNPTIENVQTGVAAFKASGADYLIAIGGGSSMDT
                     AKAIGIIIANPEFEDVRSLEGVAPTKNPSVPIIAVPTTAGTAAEVTINYVITDVEKKR
                     KFVCVDTHDIPVVAVIDPDMMASMPKGLTASTGMDALTHAIEGYTTKGAWEMTDMFHL
                     KAIELISKYLRGACENTKEGREGMALGQYIAGMGFSNVGLGIVHSMAHALGAVYDTPH
                     GVANAILLPTVMEYNAPCTGTKYRDIAIAMGVEGAENMSQEEYRKAAVEAVKKLSADV
                     GIPADLKEIVKPEDVDFLAQSAMDDACRPGNPKDPTFEDIKALYQSLM"
BASE COUNT          931 a          941 c          789 g         1025 t
ORIGIN      
        1 acaggcatcg acactgccat gatggcaggc tatggcgatt atcgtttcct gtctatcttt
       61 ctgggagttc ttaccggagt aggcggcggc gttctcagag acatcatggc cggacagacg
      121 ccctatatcc tgaaaaagca tgtgtatgcc tgtgcatctt tagccggggc tctgttttat
      181 gtatatctgt ccgacttcct gggagaaagt atctccatgg tagcagctgc attcctggtg
      241 gttctgatcc gcctgttagc tacccgctac tgctgggatc tccccaaggc tacaacggat
      301 gagatcggat agatgcatta aaaacgcata tctgtcaaat gcatcaatca aaaatgcgtt
      361 tccctcagga agaaactcct cccacgggaa acgcattttt atatttcagt atttacattt
      421 caggcattgg ccggctgtgt gtcggaaatc ccttcgtcat ttttgttgat cacatcataa
      481 gcctctgcaa tatttaacat atgatcgccg atacgctcat aatctgtaag catctcagaa
      541 taaagaatcg acgcctcaac attacagctt cccgctctca tgcggtcaat ctgattctgg
      601 cggaatttac gggtaatatc atcaatcttc tgttcgattt cctgtacctt cgccaattca
      661 aacttcttct ctcctgcagc cgcctgctcc agaagcttca ttcccttgga gcagctgtcc
      721 gccataacgg aaaactcgct ctgggcatac tcggaaaagg acagatcctt ttctaaaatg
      781 gtctgggcat actcagccag gttcatggcg tgatctccga tacgctcaat atttcccaga
      841 atagaataca tcttattcag tgtttcaata tcctttggcg tctggtccaa aacaagaaca
      901 cgggatatct ttttggaaag acggacattt gcaagatcaa tctcctcttc tctgttttca
      961 atctctccga tcccgtcttc gccgggtccc tgctctactg ccaggaagct ggcgtctacg
     1021 tttttagaag ccgcagacag catatcacgg atttcttcat ggatctggct gatggcaatg
     1081 gtggatactc caaggtgatg cttagaagcc ataagtccct gaagccagtg atcttcgtcc
     1141 ataaccttaa cctcggtatc aggaagaatc cgctctacga tcttaaccag cacactgccg
     1201 aagggaagca aaagcaggga cgttcccaga ttgaataccg tatggacatt tgcaatctgc
     1261 gccatgggat ttcccggagc caggctgatc atcacatctg taaatggagt tacaaggctt
     1321 aatacaacaa ataccactgt accgatcagg ttaaacagca gatggatcgc tgtagcgcgc
     1381 tttgcgttgc gggtcgttcc aatggaagcc agaactgccg taatacaggt accgatgttc
     1441 tgtccgaaca gaacgaatac cgcgctgtgc agatcaatga cgccgctcat ggccagagcc
     1501 tgtaagatac cgacagacgc ggaggaggac tggatcagcg ccgtgaagcc cgcgcctacc
     1561 agaatgccca gaagcggatt gctgaatttc gtcatcattt ccacaaactg cggttcttct
     1621 cttaaaggag acattgcaga gctcatcata tccataccaa taaagagtac gccgatgcct
     1681 gcaacaatac cggcaatatg ctggatcttc ttattcttta caaatacaag gatggctact
     1741 ccgacaaagg cgatcagagg agccagagct ccaacatcca gagcgatcag ctgacctgtg
     1801 atggtcgtac cgatattggc gcccatgata acccatacag cctgatttaa agtcatcaga
     1861 ccggagttaa caaagcccac taccataacc gtggtagcgg atgaggactg gatcagcgcc
     1921 gtgatcacag ctcccactaa aacgcccaga aaacggttgg aggtcagacg ctccaagatc
     1981 tgcttcatct tatttccggc tgccgcttcc agattggtac tcatcatctg cattccgtaa
     2041 aggaacagtg ccaggccgcc taaaagtcct aaaagcaatt caaatcccat gatttctcct
     2101 ttttggttca gattcagaga catttgatat cgtttttcgg ataatatcca tttatccatc
     2161 aaaacaggca caaaatataa gacatagcct gtataccgca gcagatacgc tccaccatgt
     2221 ctctgtcctc ttttcccttt ttttacattt tctttacctt ttgtttacgc attctcatta
     2281 tacgggatat ttcatttcta gtcaatatag tttccctgag gtaatgtaaa gtctgtgtaa
     2341 attatatgta aaaaggctcc ggtttccttc tgaaaaccag agcccctttc tgtgatatga
     2401 tcgttacatc agtgactgat acagcgcctt gatatcctca aaggtcggat cctttggatt
     2461 gccgggacgg caggcatcat ccattgcaga ctgcgccagg aaatctacat cctcaggctt
     2521 aacgatttcc tttaagtcag cgggaatacc tacatctgca gacagcttct ttacagcctc
     2581 tacggcagcc ttccggtatt cctcctggct catgttctct gctccttcta cgcccatggc
     2641 aatagcgatg tccctgtact tggttcccgt acagggggcg ttatactcca taacagtcgg
     2701 cagaaggatg gcatttgcaa cgccgtgagg tgtatcatat acagcgccca gagcatgcgc
     2761 catagagtgg acaataccaa gtcctacatt ggaaaagccc attccggcga tgtactggcc
     2821 cagagccata ccctcgcggc cttcctttgt attttcgcag gcgcctctta aatattttga
     2881 gatcagctcg attgccttta agtggaacat atcggtcatc tcccatgcgc cctttgttgt
     2941 gtatccctca atggcgtggg ttaaagcgtc cataccggta gatgccgtca gaccctttgg
     3001 catggaagcc atcatatccg ggtcaattac tgctactaca gggatatcat gtgtatctac
     3061 gcatacgaat ttgcgctttt tctccacatc tgtaatgaca tagttgatcg taacctctgc
     3121 tgcggttcct gctgtagtgg gaactgcaat gatcggaaca gacgggttct tggtaggagc
     3181 aacaccctct aagcttctta cgtcctcaaa ctcaggattt gcaatgatga ttccaatggc
     3241 cttggccgta tccatggagg atcctccgcc gatggcgatc agataatcag cgccggatgc
     3301 cttaaaagcg gctacgccgg tctgtacatt ttcaatcgta ggattcggct ttatattgga
     3361 gtaaatctca taggacaggc ctgcttcatc cagaacatct gtcacttttt ttgtcacgtt
     3421 aaaacggatc aaatcaggat ccgagcatac gaatgctttc ttaaaggctc ttgcctttgc
     3481 ttcatttgca atctctgcaa ttgcacctgc accgtgataa gaagtttcat ttaatacaat
     3541 tctgtttgcc atggttcctc ttctcctttt ctttctcagg atcttccctg tttgttaata
     3601 ttataacagc gcctgaaaaa aataaaagtg acaaacaggg tagtctgtca ctttctatgt
     3661 cttttttgtg aattgtcaca aaatgg
//
LOCUS       DBMDUH010000203         3621 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_9072_length_3621_cov_6.594602, whole genome shotgun sequence.
ACCESSION   DBMDUH010000203 DBMDUH010000000
VERSION     DBMDUH010000203.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9072_length_3621_cov_6.594602"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="ACZPCG_12415"
     CDS             <1..461
                     /locus_tag="ACZPCG_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007715779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR1285884.1"
                     /translation="LGGTIDLTCEDFPHGLIEDGKDPVIGLLEEARRAGVPMERMTLS
                     SDGQGSWSNYDEYGNLVEMGVTDVGNMYAQLRSLVLNGGFVLSEALSFFTSNPAKALE
                     LFPKKGCIAPGADADLLLMGEALALDTVIARGRVMMEAGELLVKGTYETF"
     gene            631..1509
                     /locus_tag="ACZPCG_12420"
     CDS             631..1509
                     /locus_tag="ACZPCG_12420"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006781199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR1285885.1"
                     /translation="MNMWTEDLVLPEELRKERLQKEPASYTAWILGKSGKTQEKKRKA
                     VIICPGGGYEHLSPREGEPIAMKYLAMGYHVFVLHYSLAPERFPTALRELALLTAKIR
                     EHADEWRIDSEGIIVSGFSAGGHLALSLGVHWDKEWLSGPLGLTPEQIRPNGLILCYP
                     VVTAGEGCHRGSVERLLWGQMLTEEEQMIPERGTEEKEMLRELISLEKQVGSHTPPVF
                     LWHTVTDQTVPVRNALILAEALIKSGVSLELHLYPVGRHGLALATEETADGQESNIEP
                     QCQSWIVLAEKWLEHF"
     gene            1556..>3621
                     /locus_tag="ACZPCG_12425"
     CDS             1556..>3621
                     /locus_tag="ACZPCG_12425"
                     /inference="COORDINATES: protein motif:HMM:NF046935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HIR1285886.1"
                     /translation="MKRRDIGKLLLIGLAAAGLQGCSQTAETTAADVEKPQVQTEAET
                     DAASETKTAEEHTWQFTYFGTSTNEKSNTMAEGGSLKNGISLTSCTVKEDGSIDKKGG
                     KFAAGEGYDGISFYYTVIDPETENFRLKADVTIDYINPKPDGQEGVAIIARDSIGEDT
                     VADCSFFTNSAAVIGSSLSYYDEDGTKTSMKDGIGYRMITGVESDTTPPEAGKLTLDS
                     EAFDKETLLTAGETYTMELRKTNTGYEAAYYDKEGNEYSRTLYGAENLNVIDNSVYAG
                     FAVARGCNATFNNIEFEVTDPAKDAPAEERPVEKENVSFTFLSSNTSGSSEYPLSFKT
                     NADGTAVISDKEGNALDTLEVKAEVPVKASYPVKEGENHFIIAFTPKEGYKINEYTEL
                     ANYGTVSFDETVSCRILSGDTIFISKDGTPDGTGTKEAPIDLATAFRYGAPGQSFVME
                     GGTYTFKEGLTVLRGVDGTEDAPIVLAPAEGERAVLDFTKEGTGLQLWGDYWHIKNID
                     VCNASDGNCGIRLSGHHNILEGIKTYNNGNTGLQVSGISEETIDMWPSDNLILNCTSY
                     NNCDEAMEDADGFAAKLTCGEGNVFRGCIAAYNADDGWDLFAKIATGKIGAVTIEDCV
                     AFKNGYVYDESGSVIDAGNGNGFKMGGSGISGHHVLKNSITYENKAKGIDSNSCPDIE
                     IYNNVS"
BASE COUNT         1049 a          764 c         1001 g          807 t
ORIGIN      
        1 agcttggggg aaccattgat ctgacctgtg aggatttccc tcatggtttg atcgaagacg
       61 gaaaggatcc tgtgatcggc cttttggagg aagcccgccg ggccggtgtt cccatggagc
      121 ggatgacttt aagctcagac ggacagggaa gttggtccaa ttatgacgag tatggaaacc
      181 tggtggaaat gggggttacg gatgtgggaa atatgtatgc ccagcttcgt tcgctggtgt
      241 taaacggagg gtttgtactt tcagaggctc tctccttttt cacttccaat cctgcaaagg
      301 ccctggagct gttcccgaaa aaaggctgta tcgcgccggg agcggacgca gatctgcttt
      361 taatgggaga agcccttgct ctggatacgg tgattgccag agggcgggtg atgatggagg
      421 caggagaact gcttgtaaag ggaacgtatg aaacatttta atctctaaag tcaagtcttt
      481 tcttaccggc aaaatactca aaaaacagaa catatctttg tgcaggatac cagtttcctt
      541 tttatgtaaa aaaatataag atagttcagg taagcgctta caatacagaa tatttagaaa
      601 acactgaatc catacacagg aggtacaccc atgaacatgt ggacagagga tctggtactg
      661 ccggaagaac tgcggaagga acgtctgcag aaggaaccgg ccagctacac agcctggatt
      721 ctgggaaaat ccggaaaaac acaggaaaag aagagaaaag cagtgataat ctgtccggga
      781 ggcggatatg agcatctttc tcccagagag ggagaaccca ttgccatgaa atatctggcc
      841 atgggctatc atgtgtttgt gcttcactat agtctggctc cggagcgatt ccccacagcc
      901 ttaagggagc tggctctgct tacggcaaag atacgggagc atgcagacga atggaggatt
      961 gattcagagg gaatcatcgt atctggcttt tctgccggag gccatctggc tttgagcctg
     1021 ggggttcact gggataagga atggctgtca gggcctctgg gattaacgcc tgagcagatc
     1081 cgccctaatg ggcttatcct ctgctatccg gtagtgacgg caggagaagg ctgccacaga
     1141 ggctcggtgg aacgtctctt gtggggacag atgcttacgg aagaggagca gatgatcccg
     1201 gagcgcggga cggaagaaaa ggaaatgctg agagagttga tttctcttga aaagcaggtc
     1261 ggttcccata cgcctcctgt gtttttgtgg cataccgtaa cagaccagac ggttccggtg
     1321 agaaacgctc tgattttggc agaggccctg ataaagagcg gcgtttcttt ggagcttcac
     1381 ctttatcccg ttggacgcca cggacttgcc ctggcgacgg aggaaacggc agacgggcag
     1441 gagagcaata tagagccaca gtgccagagc tggatcgttc tggcggaaaa atggctggag
     1501 catttttaga acaggataaa taaaacagga catcagggta cgaggaaaac aggacatgaa
     1561 aagacgagac ataggaaagc ttctactcat aggtctggca gctgcgggcc ttcagggctg
     1621 cagccagacg gcggaaacaa cagcggcaga cgtagagaaa ccacaggtac agacagaggc
     1681 agaaacggac gcagcttctg aaacgaaaac agcagaagag cacacctggc agtttacata
     1741 tttcggcaca tctaccaacg aaaagtcaaa taccatggca gagggaggaa gcctgaaaaa
     1801 tgggatttct cttacctcct gtacggtaaa ggaagacgga agcattgaca aaaaaggcgg
     1861 aaaatttgca gcaggagaag gctatgacgg catttccttt tattatacag tcattgaccc
     1921 tgaaacagag aatttccgtt taaaggcaga cgtaaccatt gactatatca atcccaagcc
     1981 ggacggccag gagggagtag ccattattgc cagagacagt attggtgagg atacagtggc
     2041 agactgctcc ttttttacaa attccgcggc agtcatcgga agcagtttaa gctattatga
     2101 tgaggacggc acaaagacct ccatgaagga cggcatcgga taccggatga tcacaggagt
     2161 agaatcggac actacgccgc cggaggcagg aaagctgact ctggattcag aggcatttga
     2221 caaggaaaca ctgctgacag cgggagaaac ctacaccatg gagctgcgaa agaccaatac
     2281 aggctatgag gcagcttatt atgacaaaga gggaaatgaa tattcccgca ccctgtatgg
     2341 tgctgagaac ctgaatgtaa ttgataattc agtttatgca ggatttgctg tagccagagg
     2401 ctgcaatgcc acttttaaca acattgaatt tgaggtaaca gacccggcaa aagatgcgcc
     2461 tgcagaggaa cgtcctgtag aaaaggaaaa tgtaagcttt accttccttt cctccaatac
     2521 gtccggttcc agcgagtatc ccctttcctt taagacgaat gcagacggta cggctgtgat
     2581 ctccgataaa gaaggaaatg ctttggatac tctggaagta aaggcagagg ttccggtaaa
     2641 ggcttcctat ccggtaaaag agggagaaaa tcattttatc atcgcattta ctcccaaaga
     2701 gggatataag atcaatgaat atacagagct tgcaaattac ggaactgttt cttttgatga
     2761 gacagtaagc tgccgtatct tatccggtga taccatcttt atttccaaag acggcacacc
     2821 cgatgggacg ggaacaaagg aagctcccat tgatctggca acggcgttcc ggtatggggc
     2881 tcccggacag agctttgtaa tggaaggagg tacctatacc tttaaggaag ggctgaccgt
     2941 tctgcgcggc gtagacggaa cagaggatgc gcccattgtt ctggctccgg cagagggaga
     3001 aagggcagtt ctggacttta caaaggaagg cacaggcctt cagctttggg gggattactg
     3061 gcatattaaa aatattgacg tatgcaatgc ctccgacgga aattgtggaa tccgcctttc
     3121 aggccatcac aatattttag agggaattaa gacctataat aacggaaaca ccggccttca
     3181 ggtcagcgga atatccgagg agaccattga tatgtggcct tcggacaacc tgatcttaaa
     3241 ctgtacctcc tataataact gcgatgaagc catggaggat gcagatggct ttgcagccaa
     3301 gctgacctgc ggagaaggaa atgtgttccg gggctgcatt gctgcctata atgcggatga
     3361 cggctgggat ctctttgcca agatcgccac aggaaaaatc ggagcagtga ccattgagga
     3421 ctgcgtggca tttaaaaacg gttatgtgta tgatgaatcc ggcagcgtga tcgatgctgg
     3481 aaacggaaac ggatttaaaa tgggaggcag cggcatcagt ggccatcatg tgttaaaaaa
     3541 cagcattacc tacgaaaaca aagcaaaggg cattgattcc aacagctgtc cggatattga
     3601 aatctacaat aatgtttctt t
//
LOCUS       DBMDUH010000204         3563 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_9248_length_3563_cov_6.409301, whole genome shotgun sequence.
ACCESSION   DBMDUH010000204 DBMDUH010000000
VERSION     DBMDUH010000204.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3563)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9248_length_3563_cov_6.409301"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="ACZPCG_12430"
     CDS             <1..543
                     /locus_tag="ACZPCG_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR1285887.1"
                     /translation="RLDDPFLNAVFEAAGKLNLPVTFHMSPETGYSYGVVDDPGLPLL
                     EACLKRHPHTQFLGHSQTFWIEMSADAPTDKESRNQWGKGPVIPGGRVPELFKKYPNL
                     YGDLSANSAGCAIMRDPAFGLEFLETYADRLFFATDMVNTEMVFPLGQWLDEQAANGA
                     LSRRAYEKICFKNAQTVFGL"
     gene            576..1424
                     /locus_tag="ACZPCG_12435"
     CDS             576..1424
                     /locus_tag="ACZPCG_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006781397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HIR1285888.1"
                     /translation="MELRTRILPRMLNSEVEAYLKENDIIIVPVGTVEMHGGMPLDSE
                     TVISEAYALKMAEACNGLVLTGLPYFYAGATASGRGTVQVSIRQGIDYLSAIAHSLLR
                     QGFKRQIYISFHGPAHMTCSPMVRDFFDETGVPILYMDLTMQMLKNAKDLFKSMDSFH
                     AITVGAYQIMNRLEDVPLTTGYYHQNPQTCKPFDDIFGLAYQSAAIGYCFGTPEDHMS
                     TTAIPDKETRKRLADEGQELIETLVNRMDLPHIAEQMRSLETYNHANEEKYPWMPSAF
                     RKNFRK"
     gene            1588..2550
                     /locus_tag="ACZPCG_12440"
     CDS             1588..2550
                     /locus_tag="ACZPCG_12440"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016359752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HIR1285889.1"
                     /translation="MKFYLAPMEGVTGYIYRNAYHAHFRPMDKYFTPFLAPKHNGGFS
                     HREINDILPEHNEGMDTVPQLLTGNSEDFLRAARELKEYGYKEVNLNLGCPSGTVAAK
                     GKGAGFLRDPEGLELFLEQVCEGMERLGLELSVKTRLGIEEPEEFDRLLSIYNRFPLK
                     ELIIHPRVLKDFYKYTPRMEAFRNAFANSAAPVCYNGDIVDRESYERLTAAYPALDAV
                     MIGRGLLADPFLIETLSGQAGTERRRTQIERLSAFHQQILEGYCQTMSGDKNVLFKMK
                     ELWAHLIHFDPEWEPYIKKIRKAQRLGEYKIITGQMFRERKAAL"
     gene            2804..3307
                     /locus_tag="ACZPCG_12445"
     CDS             2804..3307
                     /locus_tag="ACZPCG_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HIR1285890.1"
                     /translation="MFDNLTKSDIEKMQEEIEYRKLVVRKEALEAVKEARAHGDLSEN
                     FEYKAAKQDKNRNESRIRYLEKMIKTARIISDESKEDEVGLGDRVELYIPEDDETETY
                     KLVTTVRGNSLKGLISIDSPLGKAIRGHRVGERVYIKVNSDFGYEAEIRAIDKTAEAE
                     EDTLRSF"
     gene            3345..>3563
                     /locus_tag="ACZPCG_12450"
     CDS             3345..>3563
                     /locus_tag="ACZPCG_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AfsR/SARP family transcriptional regulator"
                     /protein_id="HIR1285891.1"
                     /translation="MSEEAQLKIHMMGKISAEYNGKSLPVGTALSGRVLQLFLILLYY
                     HDSGISREELLDMMYGSGEYANPAGSLRA"
BASE COUNT         1039 a          744 c          934 g          846 t
ORIGIN      
        1 aggctggatg atcccttttt aaacgccgta tttgaagcgg ctggaaaatt aaatcttccg
       61 gttacttttc atatgagtcc ggaaactggc tacagctacg gcgttgtaga cgaccctggt
      121 cttcctcttt tggaagcctg cctgaaacgc caccctcaca cgcaatttct tggtcacagc
      181 cagaccttct ggattgaaat gagcgcagat gcacctacgg acaaagagag ccggaatcag
      241 tggggaaaag gccccgttat cccaggcgga cgggttccgg aactatttaa aaaatatccc
      301 aacctatacg gagacctaag cgccaacagc gccggatgcg ccattatgcg ggatcctgcc
      361 tttggcctgg aattcttaga aacttatgca gaccggctgt tctttgccac agatatggta
      421 aataccgaaa tggtctttcc cctgggacaa tggctggacg aacaggcagc aaacggcgcc
      481 ttaagccgcc gggcctatga aaaaatctgc tttaaaaatg cccagacagt ctttggactg
      541 taagaaaacc tgttgttaaa tgaaaggaga aacatatgga acttcgcaca cggattcttc
      601 cacgtatgtt aaacagtgaa gtagaagcgt atttaaaaga aaacgacatt attatcgtcc
      661 ctgtaggcac tgttgaaatg cacggtggaa tgcctttaga cagtgaaact gtaatcagcg
      721 aggcttatgc cttaaaaatg gcggaagcct gcaacggatt agttctgact ggactgcctt
      781 atttctatgc cggcgcaact gcttccggcc gcggcacggt acaggtgtct atccgtcagg
      841 gtatcgatta tttaagcgca attgcccaca gcctgctgcg tcagggcttt aaacgccaga
      901 tttacatcag ctttcatggc cctgcccata tgacctgcag tcctatggta agagatttct
      961 ttgatgaaac cggagtcccc attctttaca tggatctgac catgcagatg ttaaagaacg
     1021 cgaaagattt atttaaaagc atggacagct tccacgccat tactgtaggc gcctaccaga
     1081 ttatgaaccg tctggaagat gtgccgctga ccaccggata ttaccatcag aatcctcaga
     1141 cctgcaagcc ttttgatgat attttcggac tggcttatca gagcgctgcc attggctact
     1201 gctttggaac tccggaagac cacatgagca ccactgccat tccggataag gaaacccgca
     1261 aacgtcttgc cgacgaaggc caggagttga tcgagaccct ggtaaaccgt atggatctgc
     1321 ctcacattgc agagcagatg cgttccctgg aaacttataa tcatgctaac gaggaaaaat
     1381 atccctggat gcccagtgca ttccggaaaa atttccggaa ataaaccata acgttatgcc
     1441 gcctgctgaa actggcttct gctgttttgg ctggcggcat tttctgtgtc tgtctatcct
     1501 gcgcagcgga caggcctgga tattcttgac tttttatctg gcgtgattta agatactgag
     1561 agcataaaaa gagaaggaga acacagtatg aaattttacc ttgctcctat ggaaggcgtg
     1621 acgggatata tatacagaaa tgcttaccat gcccatttcc gtcccatgga taaatacttt
     1681 accccctttc tggcacccaa acacaatggc gggttcagcc atagagagat aaatgatatt
     1741 ttgccggagc ataatgaggg aatggatacg gtgcctcagc ttctgactgg aaacagtgag
     1801 gattttctgc gggcagcgcg ggaattgaag gaatacggct ataaggaagt gaatttaaat
     1861 ctgggctgtc cctccggtac ggtagctgcc aagggaaagg gagcaggatt tttaagagac
     1921 cctgagggac tggagctgtt tttagagcag gtctgcgagg ggatggagcg tctggggctg
     1981 gagctttcgg tgaaaacaag actgggaatc gaagaaccgg aggaatttga cagacttctt
     2041 tctatttata atcggttccc cttaaaagag ctgatcatcc atccaagggt tttgaaggat
     2101 ttttataaat atacgcccag aatggaggcg tttagaaatg cctttgcaaa cagtgcggct
     2161 ccagtgtgtt ataatggcga tattgttgac cgggagtcct atgaacgact tacagccgcg
     2221 tatcctgcgc tggatgcagt aatgatcggc agaggccttt tagcagatcc atttctcatc
     2281 gaaacactgt cggggcaggc aggcacagag agaaggagaa cgcagataga acgtctgagt
     2341 gcattccatc agcagatttt ggaaggatac tgccaaacga tgtcagggga taagaatgta
     2401 ctctttaaaa tgaaggaact gtgggctcat ttgatccatt ttgatccgga atgggagcct
     2461 tatataaaga aaatccgcaa ggcacagcgt ctgggagagt ataagatcat aaccggtcag
     2521 atgttcagag aaaggaaagc agcactttag gctttcctat aaaatggagg gagccggcag
     2581 aaaaaggggg gaatctgccg gcttaggtat gaaaaagtgt cttcctgtat ggtaaaaggt
     2641 ctcatcaacc ttacaatcat aatatacagg ataaatgtgg acaaagtttg atcagaggct
     2701 aaataaatca taaaattatt aaagggaaaa tgaagagaat cttaagcaat tttgtggtag
     2761 aatagatatg tcagccaaag gacatataaa aggagtaaat accatgtttg ataatcttac
     2821 aaaaagcgat atagaaaaga tgcaggagga aatcgagtac cgtaagctgg ttgtccgcaa
     2881 ggaggcctta gaggcggtaa aggaagcgag agcacacggg gatctgagtg aaaattttga
     2941 atacaaagca gcaaagcagg ataagaaccg caatgaaagc cgtatccgtt atctggaaaa
     3001 gatgataaag acggccagga tcatttccga tgaatcaaag gaggacgagg taggactggg
     3061 agaccgggtg gagctgtaca tcccggagga tgatgaaaca gaaacctaca agctggttac
     3121 aacggttcgg ggaaattccc taaagggact gatcagcatt gattctccgc tgggaaaggc
     3181 catccgcggc catagagtgg gggagcgggt ttatataaag gtaaattcgg actttggata
     3241 tgaggctgag atacgggcca tagataagac ggcagaggcc gaggaggaca ccctccgaag
     3301 tttttaatct gcagacggcc ggatttacag aggggaaatg agatatgagc gaagaggcac
     3361 agttaaagat acatatgatg ggaaaaattt ctgcagagta caatgggaaa tcccttcccg
     3421 taggcacagc cctttcaggg cgtgtcctgc agttgttttt gatcctgctg tattatcacg
     3481 acagcggcat cagccgggag gaactccttg atatgatgta cgggagtggg gaatatgcca
     3541 atccggcggg gagtctcaga gcg
//
LOCUS       DBMDUH010000205         3549 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_9293_length_3549_cov_7.979118, whole genome shotgun sequence.
ACCESSION   DBMDUH010000205 DBMDUH010000000
VERSION     DBMDUH010000205.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9293_length_3549_cov_7.979118"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="ACZPCG_12455"
     CDS             <1..274
                     /locus_tag="ACZPCG_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016294614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1285892.1"
                     /translation="RNFDLAVSQADHPELFGCVRSESEGIRLVKSTARFLIRRGKPWL
                     VPGLVVKSGFKFLGYRAGKCYRLLPKWLILKLTMNREYWKKACDRA"
     gene            312..716
                     /locus_tag="ACZPCG_12460"
     CDS             312..716
                     /locus_tag="ACZPCG_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HIR1285893.1"
                     /translation="MMTVMLRVALIFVSVFTMTFMMRKIRQAKMEIEAALFWVIMALM
                     LVVFAVFPSVADFCARLLGIYATTNFLFLFMIFLLIVKLFSMTVHISQLESRQRELIQ
                     KMALEKRFKEEEEEMKDGDERRTAGTVSEQVQ"
     gene            718..2376
                     /locus_tag="ACZPCG_12465"
     CDS             718..2376
                     /locus_tag="ACZPCG_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HIR1285894.1"
                     /translation="MEKKGNILGHKWAAGPLALLFFLLWHSLDLKASVTETGNTWLFG
                     WYAGLGALVLAAAVFLGDELFIRRKMTLEALFAVSVLCLGGIYSLVLPPLSAPDEVSH
                     YISAYECSNRLMGLPSVNEKGLIQIRPEDAWLEDVADVLADDGSAEGGDEKPVILGQS
                     LTEETYRRIHEERSGENEAVHRENVQSSSSQGAGQMASSYQFSVRTTPLAYWPQALGF
                     VLARLLGLGSLVLLYLGRLFNLLFFAATGFLTLRRLPFGKTAVFAVYLLPMNLHLVSS
                     LSYDVLITAFCGYFTAVCMDLAYKADRVKWKDVVLLAFLLAVMGPCKMVYGVIAGLCL
                     LIPVKKFGGWGRWTASAAGVLGAFAAAMAAVNLGTVSMYTQAEDSYIAWAGETGYTFA
                     ELLHRPLHVLKMCYDTLAWKGTQLFEGMIGGSLGNQDPVLNTPTVIILALCTILVVLA
                     LKKPGEEVMFSTGNRLWIWFLALLLLGALMFSMLLAWTPRSASMIEGVQGRYLLPFLP
                     AVLLTFRSSRVVRTGSDDRGLLYAVMMMDLYVAVRIFAVVCLRL"
     gene            2499..3053
                     /gene="rfbC"
                     /locus_tag="ACZPCG_12470"
     CDS             2499..3053
                     /gene="rfbC"
                     /locus_tag="ACZPCG_12470"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HIR1285895.1"
                     /translation="MGKIKVTTCEIEGLYVIEPTVFKDERGYFMETYNQNDFKEAGLN
                     MTFVQDNQSMSVKGVLRGLHFQKQYPQGKLVRAVRGTVFDVAVDLRSDSKTYGKWFGV
                     TLSAENKKQFYIPEGFAHGFLVLSDEAEFAYKCTDFYHPGDEGGLLWSDPEIGVEWPI
                     EPGMELIISEKDKKWSGLKDTFKF"
     gene            3072..>3549
                     /locus_tag="ACZPCG_12475"
     CDS             3072..>3549
                     /locus_tag="ACZPCG_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1285896.1"
                     /translation="MNYAVSLLKEIITKRKLIWDLGKADFRKRFVGSYFGIVWMFIQP
                     IVTVVIYAVIFGIGFKSPPPVPGVIYVAWLVPGIVPWFFFSEALNTGTGCLQEYSYLV
                     KKVVFKVEILPIIKLISCLLVHAFFVLIMAGMYLIAGMPVRVTWIQVLYYTFAASVL"
BASE COUNT          786 a          728 c         1061 g          974 t
ORIGIN      
        1 acgcaatttc gatctggcgg tgtctcaggc agaccatccg gagctctttg gctgcgtgcg
       61 gtcggaaagc gagggaatcc gtctggtgaa atccacagcc cgttttctga tccgccgggg
      121 aaagccctgg ctggtgccgg gactggtggt aaagagcggt tttaaatttc tgggttaccg
      181 ggcaggaaaa tgttaccggc tgctgccaaa gtggctgatc ttaaagctta ccatgaaccg
      241 ggaatactgg aaaaaggcct gtgacagagc gtaaaagggc gtgaggacag gcctggaaag
      301 cggggttttt gatgatgaca gttatgcttc gtgttgcatt gatttttgta tcagtattta
      361 ccatgacatt tatgatgagg aagatacggc aggcaaaaat ggagattgaa gctgcccttt
      421 tctgggtgat catggctctt atgctggtgg ttttcgccgt ttttccctct gtggcagatt
      481 tctgcgccag acttttggga atctatgcca caacgaattt tctgtttctg tttatgatct
      541 ttctgctgat cgtaaagctt ttcagtatga cggtccatat ttcccagctg gaaagccgcc
      601 agagggagtt gatacagaag atggctcttg aaaagcggtt caaagaggag gaagaggaaa
      661 tgaaggacgg agatgaaagg cggacggccg ggactgtttc ggagcaggtg cagtaatatg
      721 gaaaaaaaag ggaatatttt ggggcataag tgggcggccg gacctttggc gctgctgttt
      781 ttcctgctgt ggcacagcct ggatctgaaa gcctctgtga cagaaacagg aaatacctgg
      841 ctgtttggct ggtatgcagg gcttggggct ttggtgcttg cagcggcggt ttttctgggg
      901 gatgagctgt ttatccgcag gaaaatgacg ctggaagccc tgtttgcggt ttcggtgctg
      961 tgtctgggcg ggatttattc cctagtgctc ccgcctcttt ctgcgcccga tgaggtaagc
     1021 cactatatca gcgcctatga gtgttccaat cgtctgatgg ggcttccttc ggtcaatgaa
     1081 aaggggctga tccagatccg gccggaggac gcctggcttg aagatgtggc ggatgtgctg
     1141 gcagatgacg gaagtgctga gggcggggat gaaaagcccg tgattttagg acagagcttg
     1201 acagaagaaa cctacaggcg gatccatgaa gaacgttccg gtgaaaacga agctgttcat
     1261 cgggagaacg ttcaaagttc ttcttctcag ggcgcgggtc agatggcaag ctcctatcag
     1321 ttttctgtgc ggaccactcc gctggcctat tggcctcagg ctcttggttt tgtccttgca
     1381 aggcttctgg gacttggaag cctggtcctt ttgtatctgg gacgtctgtt taatctgctc
     1441 ttttttgcag ccacaggctt tttgacctta aggcggcttc cctttggaaa gacagccgta
     1501 tttgccgtat atctgctgcc catgaacctc catctggtgt cttctttatc gtatgatgtg
     1561 ctgattacgg ccttctgcgg gtattttacg gcagtctgca tggatttagc ctataaggcg
     1621 gatagggtaa aatggaaaga tgtggttctt ctggcatttt tactggcagt catgggaccc
     1681 tgcaagatgg tatacggagt gatcgcgggg ctgtgccttc tgatcccggt gaaaaagttt
     1741 gggggctggg gccggtggac ggcttctgcg gcaggggtgc tgggagcctt tgcggcagcc
     1801 atggctgcag ttaatctggg aaccgtttcc atgtatactc aggcagagga cagctatatt
     1861 gcctgggccg gagagacggg atataccttt gcggagctgc tccaccgtcc tcttcatgtg
     1921 ctgaaaatgt gttatgacac gctggcgtgg aagggaacgc agctgtttga aggaatgatc
     1981 ggcggctctc tgggaaatca ggatcctgtt ttaaatacac cgaccgtgat catattggct
     2041 ctgtgtacca ttttagtggt gctggcgtta aaaaagccgg gagaagaagt gatgttttcc
     2101 acaggaaaca ggctgtggat ctggtttctg gcgctgctgc ttttaggggc gctgatgttt
     2161 tccatgctgt tagcctggac tcccagaagc gcgtccatga ttgaaggcgt tcagggaaga
     2221 tatctgctgc ccttccttcc ggcggttctg cttacattcc gcagcagccg ggtggtacgg
     2281 acgggaagcg acgacagagg gctgctttat gcggtgatga tgatggatct gtatgtggcg
     2341 gtgcggattt ttgcggtggt gtgcctgcgg ctttagagag aagcagcata aacagttgca
     2401 cccttgtgga taacgtggta aaatttcctg atagatgtgg aaaagcggct ttcgggctgc
     2461 tgaatgactc aggatggatg aaagaaggag aaagaatcat gggaaagatc aaagtgacaa
     2521 catgtgaaat tgaaggactt tatgtaattg agcctacggt ttttaaggat gagagaggct
     2581 attttatgga aacctataac cagaatgact ttaaggaagc aggccttaat atgacctttg
     2641 tacaggacaa tcagtccatg tccgtaaaag gcgttttaag gggccttcat ttccagaagc
     2701 agtatcctca gggcaagctg gtacgtgcgg taaggggaac ggtctttgac gttgccgtag
     2761 acctgcgttc cgattccaaa acctatggaa agtggttcgg cgtgacctta tctgctgaaa
     2821 acaagaagca gttttacatt ccggagggct ttgcacacgg ttttctggtt ctctccgatg
     2881 aggcggagtt tgcctacaag tgcacagact tttatcatcc gggcgatgag ggcggccttc
     2941 tctggagcga ccctgagatc ggcgtagagt ggcctatcga gccgggaatg gagctgatca
     3001 tttccgagaa ggataagaaa tggagcggtc ttaaggatac ctttaaattc tgacagtgaa
     3061 ggatacggat tatgaattat gcagtttctc tgttaaagga gattattaca aagcgaaagc
     3121 tcatatggga tcttggaaag gctgatttca gaaaacggtt cgtgggttct tacttcggta
     3181 ttgtatggat gtttattcag cccattgtga ccgtagtcat ttatgcggtg attttcggaa
     3241 tcggttttaa gtctccgccg cctgttcctg gagtcatcta tgtggcatgg ctggttccgg
     3301 gcattgtacc ttggttcttt ttcagcgagg cgttaaacac aggaacgggc tgtcttcagg
     3361 aatacagcta tctggtcaaa aaggtggttt tcaaagtaga gatcctgccg atcatcaagc
     3421 tgatttcctg tctgctggtc catgcctttt tcgtgctgat catggcggga atgtacctga
     3481 tcgccgggat gccggtgcgc gttacctgga tccaggttct ctattatacc tttgcagcct
     3541 ctgtgctgg
//
LOCUS       DBMDUH010000206         3452 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_9546_length_3452_cov_6.316025, whole genome shotgun sequence.
ACCESSION   DBMDUH010000206 DBMDUH010000000
VERSION     DBMDUH010000206.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9546_length_3452_cov_6.316025"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            104..436
                     /locus_tag="ACZPCG_12480"
     CDS             104..436
                     /locus_tag="ACZPCG_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009215075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR1285897.1"
                     /translation="MARKYDQEYKVQAVKLAKEIGGTKAAKELGIPEGTIHTWLKAVR
                     NGKLDIGEGSHTPASAMSLSEEITMLRKRVKEQDKEIRRLKEENEFLEEASAFFAASR
                     RKSAKTRE"
     gene            433..1341
                     /locus_tag="ACZPCG_12485"
     CDS             433..1341
                     /locus_tag="ACZPCG_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HIR1285898.1"
                     /translation="MMFLALKTEDGRITGKIAFYCRMLGISRQGFYKYLANKDRPWKY
                     QDLAQAMEEIVSEDECNDTYGRSRMYQALLLKQPEGVRIPSERTVYRVMNQMGLSHRP
                     KRKPNGITKADRESMKSEDLLKRDFHANAPLEKCITDITEIPASNGKLYVSAIFDCFD
                     LSVLGLAMETTMKADLCVHTLENALTAYPALEGAVIHSDRGTQYTSETYRQAIWENHI
                     HQSMNSAGGRCHDNARCESMWARMKTELLYNRYDTKQMTVEDLKELIWRYFFSYWNNR
                     RICSANGVLPPMIKRRQYYEALEMAA"
     gene            1549..2073
                     /locus_tag="ACZPCG_12490"
     CDS             1549..2073
                     /locus_tag="ACZPCG_12490"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HIR1285899.1"
                     /translation="MFTIILGDITKEPVDAIVNAANTSLLGGGGVDGAIHRAAGPELL
                     AECRTLHGCETGQAKITKGYRLPAKYVIHTPGPIWRGGIMGEEQLLQSCYENCLRLAV
                     ENGCRSVAFPSISTGVYRFPLETASEIAVRTILEFLEAHPDMEVRMICFDQETLGYYE
                     SALNEQTKIANEED"
     gene            2079..2639
                     /locus_tag="ACZPCG_12495"
     CDS             2079..2639
                     /locus_tag="ACZPCG_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009295913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIR1285900.1"
                     /translation="MRKNFGAKPWTYPQPVLMIATYGEDGTPDVMNAAWGGISDDTQI
                     SICVSADHKTTENILARKAFTVSMAEYAYIKECDYVGIVSGNDVPDKFAKAGFHAEKS
                     KFVDAPIIKELRMALECKLVSYDPETCRLIGEIVNVSADESVLDKDGKIDPDRLQPIT
                     FDPVHHEYRILGERVGRAFRDGLELK"
     gene            complement(2930..3244)
                     /locus_tag="ACZPCG_12500"
     CDS             complement(2930..3244)
                     /locus_tag="ACZPCG_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007157196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HIR1285901.1"
                     /translation="MISRKPTHPGVVLLEDVIKPLGLTITDAARMLGVSRKALSEFVN
                     EKAILSPDMAIRIAKATNTSPESWMNMQQKLTLWEAEQHLPKNVIPFRSVRSEEAAAL
                     QM"
BASE COUNT         1023 a          708 c          881 g          840 t
ORIGIN      
        1 caatcgtata gtcgctttca aacaatgatg tggtataaaa aaagttgaca gcttgttctc
       61 aaggatttca taataaaatc aagagaacaa ggaggtattc aaagtggcac gtaaatatga
      121 ccaggaatac aaagtacagg cagtaaaact tgccaaagaa atcggtggca ctaaagccgc
      181 gaaagaatta ggaatccctg aaggaaccat ccacacatgg ttaaaagccg taagaaacgg
      241 taaactggat atcggggaag gttcccatac ccctgccagt gcaatgagtc tttcagaaga
      301 aattaccatg ctgcgtaagc gggttaagga gcaggacaaa gaaatccgac gtctgaagga
      361 ggaaaacgaa tttttggagg aagccagcgc ttttttcgcc gccagccgtc ggaagtcagc
      421 aaaaaccaga gaatgatgtt tctggctttg aaaacagagg acggcaggat taccggaaaa
      481 atcgcatttt attgccgaat gcttggtatc agccgacaag gcttctataa atacctcgct
      541 aacaaagacc gtccctggaa gtatcaggat ctggcacagg ccatggaaga aatcgtcagt
      601 gaggacgaat gtaacgatac ttatggacgg agccgtatgt atcaggcgct gctgctgaaa
      661 cagccagagg gagtgcgtat tcccagtgag cggaccgtat accgggttat gaaccagatg
      721 ggtctaagcc atcggccaaa gagaaagccg aatgggatca caaaagcaga tcgggaatcc
      781 atgaaatcgg aggatctgct gaaacgggat ttccatgcaa atgccccatt ggaaaaatgt
      841 attacagaca tcacggagat cccggcgtcc aatggaaaac tgtatgtatc tgcgattttc
      901 gactgctttg atcttagcgt ccttggactg gcaatggaaa caactatgaa agcagatctg
      961 tgtgtccata cactggaaaa tgctttgact gcttatcccg cactggaagg tgcagtcatc
     1021 cacagtgacc gtggaacgca gtataccagc gagacctacc gtcaggccat ctgggagaac
     1081 cacatccatc aaagcatgaa cagtgccgga ggacgctgcc atgataatgc acggtgtgaa
     1141 agtatgtggg ctagaatgaa gacggagctt ctctataacc gctatgacac aaagcagatg
     1201 acagtagagg atttaaagga gcttatctgg agatacttct tcagttactg gaataaccgg
     1261 aggatctgct cagccaatgg cgtccttcct cctatgataa aacgcaggca gtattatgag
     1321 gctttggaga tggcggcata gtcagtgata tccttgagga aaatgtgtca actaatattg
     1381 acaatatcac aatgaaaaga ttcgactttt tggaagcgaa agtatttgaa agaaaattta
     1441 gctgcgtttg ctgtgctccg cagctgagag tgttctttcc tattaaaatg tggtatactg
     1501 acagaaaacg gaagaatcga aaacaatcta taataagaag gtgatccaat gtttacaatc
     1561 attttgggag atattacgaa agagccagtg gatgccattg taaacgccgc caacaccagc
     1621 cttctgggcg gcggaggcgt ggacggcgcc attcatcggg ccgcagggcc ggaacttctg
     1681 gcagagtgcc ggacgctcca cggctgtgaa acgggacaag ccaaaataac aaagggatac
     1741 cgccttccgg caaagtatgt gatccacacg cctggaccga tttggagagg cggtatcatg
     1801 ggcgaggaac agcttttgca gagctgctac gaaaactgcc tgaggcttgc agtggaaaac
     1861 ggatgccgga gtgtggcatt tccgtctatc agtacgggag tttaccgatt ccctttggaa
     1921 acggcttctg aaattgccgt caggacgatt cttgagttcc tggaagccca tccggacatg
     1981 gaagtgcgga tgatatgctt tgaccaggag acgcttgggt attatgagag tgctttgaat
     2041 gagcagacta agattgctaa tgaggaggat tgatttcgat gagaaagaat tttggagcaa
     2101 aaccatggac ttacccgcag ccggtgttga tgatcgcaac ctacggggag gacggcacgc
     2161 cggatgtgat gaatgcagcc tggggcggca tcagtgatga tacgcagatt tccatctgtg
     2221 tcagtgcgga tcataaaacc acagagaata ttcttgcaag aaaggcgttt accgtcagca
     2281 tggcagagta tgcgtatatt aaggaatgtg actatgtggg gattgtgtcc ggaaacgatg
     2341 tgccggacaa gtttgcaaaa gccggctttc atgcagagaa atcgaaattc gtggacgctc
     2401 cgatcatcaa ggagcttcgc atggcgctgg aatgtaaact ggtcagctac gatccggaaa
     2461 cctgccggct gattggcgag atcgtcaatg tcagtgcgga cgagagtgtt ctggataagg
     2521 acggaaagat tgatcccgac agactgcagc ccattacctt tgatccggta catcatgagt
     2581 acagaattct tggagaacgg gtaggcaggg cgtttcggga cggattggag ttaaaatagc
     2641 agaatgcgcc gatcatttaa cgagggatcg cttggcagga gatacgctga accggtctct
     2701 tgttttttac caataaacgc aaaagatttc cataattaaa acagaaggat tgtatatgac
     2761 ttcaagtagc ggtaagcatt tcttctataa ataagaagaa acacgaaaaa aaacaccaat
     2821 ttatatgaaa tagttagaca atccatacca attttgttat aattgcaaca tgaggatcag
     2881 aagaagctgt tttggaacag ggggctccat caatatggtg gagccatttt tacatctgta
     2941 gcgcagcagc ctcttcagac cgtacggatc gaaatgggat aacattcttt ggaagatgct
     3001 gttctgcttc ccagagtgtc agtttctgct gcatattcat ccagctttcc ggcgatgtgt
     3061 ttgtcgcttt tgcaatgcga attgccatat caggacttaa tatcgctttt tcatttacaa
     3121 attctgataa tgctttccgg cttacaccaa gcattctggc tgcatcggta atggtaagcc
     3181 ctagtggttt tataacatct tccaagagga caactcccgg atgagttggt tttctgctta
     3241 tcatacatgt tcctccttct aatgatagtc ctggtaatcc acgagatgcg catcctgtcc
     3301 ttcgaaataa aatgttaatc gccaattgcc attctgctca atcttctgga aagatattca
     3361 agtgaagtgc aaatatagta aatattatcc ttttctgttt ctgtcataaa tgaaagagct
     3421 aatcccccag ctatgctggg gcgaatagtg ta
//
LOCUS       DBMDUH010000207         3437 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_9593_length_3437_cov_4.133393, whole genome shotgun sequence.
ACCESSION   DBMDUH010000207 DBMDUH010000000
VERSION     DBMDUH010000207.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3437)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9593_length_3437_cov_4.133393"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="ACZPCG_12505"
     CDS             <1..687
                     /locus_tag="ACZPCG_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HIR1285902.1"
                     /translation="NAVPVQEVVFVMDRFGYCKLLDKSTYERNQETVDTEQVYVKRCL
                     NTDKICLFTASGTLHQIKALDIPSGKLRDKGVPIENLSKYDGREDRICLLTCAQELKG
                     RKLVFATKLAMVKQVPGEEFETNNRMVAATKLQEGDEVLSVALVEEQTATVVLQTTGG
                     YFLRFPLEDISVLKKASRGIRGIKLSKNEELEQLYLLGDEPYAITYKDREINLSGMRI
                     TKRDAKGTKK"
     gene            842..1927
                     /gene="asd"
                     /locus_tag="ACZPCG_12510"
     CDS             842..1927
                     /gene="asd"
                     /locus_tag="ACZPCG_12510"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HIR1285903.1"
                     /translation="MERKLRVGILGATGMVGQRFIALLENHPWYEVVTVAASPRSAGK
                     TYEEAVGGRWKMTTPMPEAVKNLIVMNVNEVEKVAAGVDFVFSAVDMTKEEIRAIEEA
                     YAKTETPVVSNNSAHRWTPDVPMVIPEVNPEHFDVIEFQKKRLGTTRGFIAVKPNCSI
                     QSYAPVLTAWKEFEPYEVVATTYQAISGAGKTFKDWPEMEGNIIPYIGGEEEKSEQEP
                     LRIWGHIEDGVIVKAQSPVITCQCIRVPVLNGHTAAVFVKFKKKVTKEELIEKLESFK
                     GLPQELKLPSAPAQFIRYMEEDNRPQVSLDVDFENGMGISVGRLREDTVYDYKFVGLS
                     HNTVRGAAGGAVLCAELLTAKGYIKEK"
     gene            1972..3036
                     /locus_tag="ACZPCG_12515"
     CDS             1972..3036
                     /locus_tag="ACZPCG_12515"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HIR1285904.1"
                     /translation="MAITKQAFGVMPSGETVYKYTLTNHHGVSADFITLGGTWVTMNV
                     PDKKGKMADVILGYDNLEGYLSNNPHFGAIIGRNANRIGGAVITIDGKDYKLEANNGP
                     NNLHSAPDLYHRRLWDCEAAETDEGSCLTFSLKSPDGDQGYPGNASIQVSYTLTEDDS
                     IRIHYHMICDADTIANFTNHCYFNLAGHDGGSVMDQEVWLAASKFTPADEVSIPTGEI
                     LPVEGTPMDFTEMKPLGRDIDEPYEALILGHGYDHNWVLDHDPGILSLAARAKDPKSG
                     RMMETYTDLPGIQLYTANFLNDPVPGKGGVRYEGHHAFCFETQHFPDACHKPEFPSPI
                     LKAGEVYDTTTVYRFYTETE"
     gene            3296..>3437
                     /locus_tag="ACZPCG_12520"
     CDS             3296..>3437
                     /locus_tag="ACZPCG_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HIR1285905.1"
                     /translation="MSKSLYIAEKPSVAQQFADALKIRGRRGDGYIESEDAIVTWCVG
                     HLV"
BASE COUNT         1004 a          691 c          925 g          817 t
ORIGIN      
        1 aatgcagttc cggttcagga ggttgttttt gtgatggatc gtttcggcta ttgtaagctt
       61 ttggataagt ctacttacga acggaatcag gaaacggtgg atacggagca ggtatatgta
      121 aagcgctgcc tgaatacgga taagatctgc ctgtttacgg cctcaggaac actgcatcag
      181 atcaaggcat tggatatccc ctcaggaaag ctgagagata agggtgtgcc cattgaaaac
      241 ttgagcaaat atgatggaag agaagaccgg atctgtcttc ttacctgcgc ccaggagctg
      301 aaaggaagaa agctcgtgtt tgccacaaag cttgctatgg taaagcaggt gccgggagaa
      361 gagtttgaga ctaataaccg catggtggca gctaccaagc ttcaggaagg ggacgaagtt
      421 ctgtctgtag cgctggtaga ggagcagaca gctaccgtcg tgcttcagac cacgggggga
      481 tatttcctga ggttcccgtt ggaggacatt tccgtactta aaaaggcatc cagaggtatc
      541 agaggcataa agctttccaa aaatgaagag ctggagcagc tttatctgct gggcgatgag
      601 ccgtatgcca ttacctataa agacagggag atcaatttga gcggaatgcg gatcactaag
      661 cgggatgcga aaggcacaaa gaagtaaaaa gtacgccata gaaatacaaa tgtgcgcaaa
      721 acacgaattt ctggttgcat ttttctttac tttggtatag gataggaaat aatcctacag
      781 gggaggcttc ccatatcatc agcggaagga ttatgaaaaa gaaagacgag gagagcgaga
      841 catggaaaga aaattaagag tgggcatatt aggcgcaaca ggtatggtag ggcagaggtt
      901 tatcgccctg ctggaaaatc atccatggta tgaggtagtg acagtggctg ccagccccag
      961 atctgccgga aagacttacg aagaggctgt aggaggccgc tggaagatga caactcctat
     1021 gcctgaggcg gtaaagaacc tgatcgtaat gaacgtaaac gaggtagaaa aggtagctgc
     1081 aggcgttgat ttcgtattca gtgcagtgga tatgacaaag gaagagatcc gtgccattga
     1141 ggaggcttat gccaaaacgg agacacctgt ggtttccaac aacagcgctc accgctggac
     1201 gccggacgtt cctatggtaa tcccggaggt aaatccggag cactttgatg tgatcgaatt
     1261 tcagaaaaag cgtctcggca ccaccagagg ctttattgcc gttaagccca actgttccat
     1321 tcagagctat gctcccgtac tgactgcctg gaaggaattt gagccttatg aggtagtggc
     1381 tactacatat caggcgattt ccggcgccgg aaagactttt aaggactggc cggagatgga
     1441 aggaaatatt atcccctata tcggcggaga ggaagaaaag agcgagcagg agcctctgcg
     1501 tatctggggc catattgaag acggtgtgat cgtaaaggcc cagtctcctg tgatcacctg
     1561 ccagtgcatc cgcgtgcctg tattaaacgg ccacacagcg gctgtgtttg taaagtttaa
     1621 aaagaaggtt acaaaggaag aactgatcga aaagctggaa agctttaagg gccttcctca
     1681 ggaattaaag cttccaagtg cccctgctca gtttatccgt tatatggaag aggataaccg
     1741 tcctcaggta agcctggatg tggactttga aaacggaatg ggaatttctg tgggacgttt
     1801 gagagaggat acggtttacg attataaatt tgtaggactt tcccataata ctgtccgcgg
     1861 cgctgccggc ggagccgttc tctgcgcaga gcttctgact gcaaagggat atattaagga
     1921 aaaataagca aaggacgcgc tttgcataaa cggaaggggt aaatagcaat tatggcaatt
     1981 acaaaacagg cattcggtgt gatgccgtcc ggagaaacgg tttataagta tacgctgacg
     2041 aatcatcacg gagtcagcgc agactttatc acccttggag gaacctgggt gaccatgaat
     2101 gtgccggata aaaagggaaa aatggcagat gtgatcctgg gatatgataa cctggagggg
     2161 tatctgtcca acaatcctca ttttggcgca attatcggaa gaaacgccaa ccgtatcggg
     2221 ggggcagtga ttaccattga cggaaaggac tataagctgg aagcgaacaa cgggccaaat
     2281 aatcttcaca gtgctccgga tctctatcac agacgccttt gggactgtga ggccgcagaa
     2341 accgacgagg gaagctgcct tactttttct ttaaagagcc cggacggaga tcagggatat
     2401 ccgggaaacg catccatcca ggtaagctat acgctgacag aggatgacag catccggatc
     2461 cattaccata tgatctgtga tgcagatacc attgccaact ttacaaacca ctgctacttt
     2521 aatctggcgg gccatgatgg cggttctgtt atggatcaag aggtatggct ggctgcttct
     2581 aagtttactc ctgcagatga ggtatccatt cctaccggag agattcttcc ggtagaggga
     2641 acccctatgg actttacgga aatgaagcct ctgggccgtg atattgatga gccatatgaa
     2701 gcattgatcc tgggacatgg ttatgaccat aactgggtat tagatcacga tccggggatt
     2761 ttatctcttg cagcaagagc gaaggatcca aagagcggac gtatgatgga gacgtatacg
     2821 gatcttcccg gtatccagct ttatacagcc aatttcctga atgatccggt tcccggaaaa
     2881 ggcggtgtcc gctatgaggg acaccatgca ttctgttttg aaacccagca tttcccggat
     2941 gcctgtcata aacctgagtt tccgtccccg atcttaaagg caggagaggt gtacgataca
     3001 acgacggttt ataggttcta tacagaaacg gaataaaaag gcagatatta aaattcacat
     3061 agaaaagggc tttttgatga gataaaaact cacaaagggc tctttttatt tgtgtggatt
     3121 ccatcaaaga taaaaaaatc gcgagatttt atctcgcaaa ctgcaaaact acaaagaatg
     3181 gttgaattta acatgagatt tttactcaat tattgctata ttttctattt aatggtatac
     3241 tagctaaata tgcctgcatg caggcattta tattaaaagg aaaatgaggg atcgcatgtc
     3301 aaaatcattg tacatcgcgg aaaagcccag tgtagcccag cagtttgcag atgcattaaa
     3361 gatccgggga cggcggggag acggatatat tgaatccgag gacgccattg taacctggtg
     3421 tgtgggccat ctggtga
//
LOCUS       DBMDUH010000208         3432 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_9607_length_3432_cov_4.153708, whole genome shotgun sequence.
ACCESSION   DBMDUH010000208 DBMDUH010000000
VERSION     DBMDUH010000208.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3432)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3432)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3432
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9607_length_3432_cov_4.153708"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="ACZPCG_12525"
     CDS             <1..384
                     /locus_tag="ACZPCG_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007707192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation T C-terminal
                     domain-containing protein"
                     /protein_id="HIR1285906.1"
                     /translation="TAFAGQYADAMAQSTGLIVCITDRDQVVAVAGGSRKELLQKTIS
                     RSLEQAINDRITYQAGKDEKNVIQLVDEELEGITAQVIAPIICEGDAIGSVSLMSREP
                     KARFGEMEMKLAVTAAGFLGRQMES"
     gene            943..2055
                     /locus_tag="ACZPCG_12530"
     CDS             943..2055
                     /locus_tag="ACZPCG_12530"
                     /inference="COORDINATES: protein motif:HMM:NF024799.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR1285907.1"
                     /translation="MSRSTTTLKQIAETAGVSITTVHRVLNGKEGCGEQLRTRIMEIA
                     KQQGYEINYVASSLRKKPIHIAVIFPKSDADSHLYVQEILNGYFQEREEVEPYNIIFQ
                     EYYYPAYDLESMVFLSCLNNIYQERPFRYDGVIVYKEDLGDDRRYTAILNRIMGKRIP
                     VVILQKCRDDTQYSCLVGPDEELAGKMAAELMDKMTVGEGNIKIFSQDLPFADKNAAV
                     FAEELKCRRPELRCSITALKLNEEQSERIARELSEGEYPSGIYFTCARHTAAFLRIDP
                     KIRRNAGTVIGSELFEESCQALQTGALDAVIDKKPFSVGYQALKLLFNNIVKNEKLPV
                     RYNVLPRIIIQSNSYVYYRRRKEKYGSSEETEYSIY"
     gene            2018..>3432
                     /locus_tag="ACZPCG_12535"
     CDS             2018..>3432
                     /locus_tag="ACZPCG_12535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1285908.1"
                     /translation="MEAAKKRNIAYIENVKNAPAFGLRDKIGYLCGDLGFNSLQVIVN
                     SYLMLFCVNILGMNAVHFAGIVFICKALDALNDTFIGRTVDRRAPAKNGKMKPYLLWF
                     AVPYAIFTLILFMNVHAMPYAAKIAWVLIIYFFWGIIGTFINVPYGAMSNIITTNQIE
                     RTELSNFRSIGSFAASMGTTTIAPLLLFDQNNDPIAGRFILLSVILGVFCTVCLFLAH
                     TLIHERVLVEEVKVNENGEKINYLQVAKSFLHNRVMIAVVASYVIVKLFIQPVSTLNQ
                     YVFMTYFQDTSSLAMASLTTMGPMLLGMVFLKPLVKRFGKKALVTWPLLGSALMHGAN
                     MLLPLGPKEWIFCQLMASAFITCLNLLLWSLIADAVDYQAYLTGQRNDGTVYATITFI
                     VFFAASASTSIIALLLEAVGYDPTLGSMGQLAGVPEKIKMLGGAWPMIGALLSFVCFK
                     FIYNVSDARMREISEELRRKS"
BASE COUNT         1036 a          605 c          851 g          940 t
ORIGIN      
        1 actgcttttg caggccagta tgcagatgcc atggcccagt ctaccggact gatcgtctgt
       61 attacagacc gggatcaggt agtggctgtg gcgggcggct ccagaaagga gctgctgcaa
      121 aagactataa gccgttctct ggagcaggcc atcaacgacc gtatcaccta tcaggccgga
      181 aaggatgaaa aaaatgtgat tcagctggta gatgaagagc tggagggcat taccgcccag
      241 gtgattgctc ctattatctg tgaaggggac gccattggtt ctgtatccct tatgagccgg
      301 gaaccaaagg cacggtttgg cgagatggaa atgaagctgg cagtcactgc cgccggattt
      361 ctgggacggc agatggaaag ttaagaaatt aagaaaggaa agacgatgaa tgttcgcctt
      421 tccttttttt attccccttg atcattcgga gaatgaagct tgtgtttttt cctgtattgc
      481 aggggactgc agtcggaaag ggctttaaag gttctggaaa ataagtttga tatgaacaac
      541 cgtttcaagg tcattctggc aggagggagg cagtatggag gcttctaaaa cggatgtaag
      601 aaacaaaaag atgagaacac atttttgagt aaatttttcg gaaaaaaaag aaagggaaaa
      661 atgggaacga agcggatcgt ccggacagaa ataggagaag gatgtgcgat gagggaaaga
      721 atgtttccga aagattgaaa aaacaggata aagaagggga aaatacggaa aaaattgtat
      781 ataatgcaca aaaacgatgt aaaattattg tggaaaaata cgtttacatt cccatatata
      841 acaggtaaca tagaaattgt aacccaaaag taaacgataa cgaaaacatt tacgttaacg
      901 tttacttaac agttttcgct ggccagcaaa ggaactgaaa aaatgagcag atcaacaacc
      961 acactgaagc aaattgcaga aacagcaggc gtttctatca caactgtaca ccgggtgctg
     1021 aatggcaagg aaggctgcgg cgaacagctg cggacacgca ttatggaaat tgcaaaacag
     1081 cagggatacg agattaatta tgtagcgtct tcccttcgaa aaaaaccaat ccatattgcg
     1141 gtgatttttc caaaaagcga tgcagacagc catctgtatg ttcaggagat tctgaatgga
     1201 tattttcagg aaagagaaga ggttgaaccc tataatatta tttttcagga atattattat
     1261 ccggcttatg atttggagag tatggtattt ttatcctgtc tgaataatat ttatcaggaa
     1321 cggccatttc gctatgatgg agtaatcgtt tataaagagg atctgggaga tgacaggcgg
     1381 tataccgcta ttttgaaccg tattatggga aaacggattc ctgttgtcat tctgcagaaa
     1441 tgccgggatg atacgcaata ctcctgtctg gtaggtccgg atgaagagct tgcaggtaaa
     1501 atggccgcag agcttatgga caagatgaca gttggggagg gaaacataaa gatattcagt
     1561 caggatcttc cgtttgcaga taagaatgca gctgtatttg cagaagaatt aaaatgcaga
     1621 cgtccggaac tgaggtgcag tataactgca ctgaaactga atgaagagca gtcagaacga
     1681 atcgcccggg agctgtcaga aggagagtac ccgtcaggca tatactttac atgtgcccgt
     1741 catacagcgg catttctcag aattgatccg aagatacgaa gaaatgcagg aacggtgatt
     1801 ggaagcgaac tgtttgagga aagctgtcag gcattgcaga caggcgcgct ggacgctgtg
     1861 attgataaaa aacccttcag tgtaggatat caggctctga aactgctgtt taataatatc
     1921 gttaagaatg aaaagcttcc tgtaaggtat aatgttcttc cgcgtattat tattcagtcc
     1981 aacagttatg tctactacag aagaaggaaa gaaaagtatg gaagcagcga agaaacggaa
     2041 tatagcatat attgaaaatg tgaaaaatgc tccggcattt ggactgagag ataagatagg
     2101 atacctgtgc ggcgaccttg gctttaacag cctgcaggtt attgtaaaca gttatctgat
     2161 gttgttctgt gttaatattt taggaatgaa tgcagttcat tttgcaggca ttgtgtttat
     2221 atgcaaggcg ttggatgcgt taaatgacac atttatcggg agaacggtgg atcgccgtgc
     2281 acctgctaaa aatggaaaaa tgaagccgta tctgctgtgg tttgcagttc cgtatgccat
     2341 atttacgctg atactattta tgaatgttca cgctatgccg tatgcagcaa aaatagcatg
     2401 ggtattgatc atctattttt tctggggtat tataggtacc tttatcaatg ttccttatgg
     2461 agcaatgtcc aatattatta ctacgaatca gattgagaga acggagcttt ccaatttccg
     2521 aagcatagga agttttgcag ccagcatggg aacgacaacc attgcgcctc tcctgctgtt
     2581 tgaccagaat aatgatccaa ttgccggaag atttattctg ctgtctgtta ttttaggagt
     2641 gttctgcaca gtatgtctgt ttttggcaca taccctgatt catgaacgcg ttctggtgga
     2701 agaagttaag gttaatgaaa atggtgagaa gattaactat cttcaggtag caaaatcctt
     2761 tctgcataat cgggttatga ttgcggtagt tgcatcatat gtgatcgtta aattatttat
     2821 acagccggta tctactttaa accagtatgt atttatgaca tattttcagg acacatccag
     2881 tcttgctatg gcatctctta ctacaatggg gccaatgctt ttgggaatgg tatttttgaa
     2941 gcctcttgta aaacgctttg gaaaaaaggc gcttgttaca tggccgcttc tgggatcggc
     3001 acttatgcat ggagccaata tgcttcttcc tcttgggccg aaagaatgga ttttctgtca
     3061 gcttatggca tctgcattta ttacctgcct gaatctttta ttatggtctc tgatcgcaga
     3121 cgcagtagat tatcaggcgt atctgaccgg acagagaaat gatggaacgg tctacgcaac
     3181 gattacattt attgtatttt ttgctgcaag tgccagtaca tcgatcattg ctcttctttt
     3241 ggaggcagtt ggatatgatc cgactctggg ttccatggga cagctggcag gcgtaccaga
     3301 aaaaatcaaa atgctgggag gcgcgtggcc tatgattggc gctcttcttt catttgtctg
     3361 ctttaaattt atttacaatg taagcgatgc acgaatgaga gaaatcagcg aggaacttcg
     3421 cagaaaaagc ga
//
LOCUS       DBMDUH010000209         3428 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_9621_length_3428_cov_6.068530, whole genome shotgun sequence.
ACCESSION   DBMDUH010000209 DBMDUH010000000
VERSION     DBMDUH010000209.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9621_length_3428_cov_6.068530"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            169..327
                     /locus_tag="ACZPCG_12540"
     CDS             169..327
                     /locus_tag="ACZPCG_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HIR1285909.1"
                     /translation="MTCVIVTHDMNLARTAGHIIVLDRGTVCGEGTHDTLYESCSVYR
                     NLYDKAIS"
     gene            complement(379..1158)
                     /locus_tag="ACZPCG_12545"
     CDS             complement(379..1158)
                     /locus_tag="ACZPCG_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012738607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIR1285910.1"
                     /translation="MIKIAFFDIDGTLLRFRHNDLSEPVRSALKQLQKQGILLCMATG
                     RSYPFIPRFEGIEFDVILSFNGSYVRNREEVIRKEPLTREDVNCIIGNLNKKKRAIAI
                     SNEAVVVTNGTDPDLEQYFAFGNEEMIISEKFNDISREDIYQIMCSCTKDEYEEILEG
                     TKRAQITSWWDRAADIIPSNSGKGIAVMDVLDYYGISREKSIAFGDGKNDIEMLRAVG
                     IGVAMGNSCTEVKDSADESCLSVEEDGIYHYFLERNMIEAI"
     gene            complement(1244..2044)
                     /locus_tag="ACZPCG_12550"
     CDS             complement(1244..2044)
                     /locus_tag="ACZPCG_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005541649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HIR1285911.1"
                     /translation="MNIMAIVMAAAVIAVVGIVVGIGLGLFGEKFKVEVDEKELAIRE
                     ELPGNNCGGCGFAGCDALAKAIANGQAPANACPVGGAPVGEKIASILGVDAGAADKKV
                     AFVRCKGTCDKAKTLYTYTGIEDCAKATVVPGGSPKACSYGCMGFGSCVKACQFDAIH
                     VIDGVAVVDKEKCVACGKCVAACPKGLIQLVPYSAKHLVQCSSHDKGKDVKAACQAGC
                     IGCTLCTRQCESDAIHMDNNLAVIDYDKCTNCGKCAEKCPAKVILEQE"
     gene            complement(2060..2635)
                     /locus_tag="ACZPCG_12555"
     CDS             complement(2060..2635)
                     /locus_tag="ACZPCG_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002600142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex protein RnfA"
                     /protein_id="HIR1285912.1"
                     /translation="MKELLVIAVSSAFVSNVVLSQFLGICSFVGVSKKVETAAGMGGA
                     VIFVITIASIAASLLYKLILQPLGLDYLKTIVFILVIAALVQIVEMFLKKCMVSLYNA
                     LGVFLPLITTNCAVLGVALTNVQNDYTIIESVVNGVATAFGYTVAIVLLAGIRERIEG
                     NDIPYNFQGSPIVLVTAGLMAIAFFGFSGLL"
     gene            complement(2649..3326)
                     /gene="rsxE"
                     /locus_tag="ACZPCG_12560"
     CDS             complement(2649..3326)
                     /gene="rsxE"
                     /locus_tag="ACZPCG_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007157840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HIR1285913.1"
                     /translation="MNKYTERIYNGVWKENPILIQMLGLCPTLAVTTSATNGAGMGLS
                     TTAVLVAANIVISALRNIIPARVRLPGEIVVVASLVTIVDMLMEGFTPSLYSALGIYI
                     PLIVVNCIILGRAEAFAMKNKPLISAFDGLGMGLGFTIALILIGGARELLGAGTVFGI
                     QVMPASYEPAAIFILAPGAFFVLACLTALQNKLKLPCATNGSVKDKKKRKGCSGDCAS
                     CKEKTEE"
     gene            complement(3339..3425)
                     /locus_tag="ACZPCG_12565"
     CDS             complement(3339..3425)
                     /locus_tag="ACZPCG_12565"
                     /inference="COORDINATES: protein motif:HMM:NF016121.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285914.1"
                     /translation="MSGATYTSNAVTGAANAAIYFVENCIGE"
BASE COUNT          847 a          946 c          743 g          892 t
ORIGIN      
        1 catcacacag gatacctccg ggatcagcta cggcatttcc agctgcattg gtgtggttgt
       61 gccgtggctg attctggtat cccttgtaac tggccgattt gcttttcggt tctccgccgg
      121 agaacggcaa agactggctc ttgcaaggac gctggtccgg gatccccagt gacttgtgtc
      181 atcgtcaccc atgatatgaa tctggcccgg acagccggac atattatcgt tctggaccgg
      241 ggcactgtct gcggagaggg gacccacgat acgctctatg aatcctgcag cgtataccgg
      301 aatctctatg acaaagccat ttcctaaaaa gtaaaccagc aggacgtctc tatcgatatc
      361 ccgctggttt acttattttt aaattgcctc aatcatattt cgctctaaaa agtaatggta
      421 aattccgtcc tcctcaaccg acaggcagct ttcatctgcg ctgtccttta cctctgtaca
      481 ggaatttccc atcgctacac caatccccac tgccctcagc atctcgatgt cattcttccc
      541 gtctccgaac gctatggatt tctcccttga aatcccatag taatccaata catccattac
      601 ggcaattccc tttccgctgt ttgacggaat aatatccgcc gccctgtccc accaggaagt
      661 gatctgtgca cgtttagttc cttcaagaat ttcctcgtat tcatcctttg tgcagctgca
      721 cataatctga taaatgtctt ccctggaaat gtcattgaat ttttcggaaa ttatcatttc
      781 ttcatttccg aatgcaaagt actgttccag atccggatcg gttccatttg tgaccaccac
      841 agcttcattg ctgattgcaa ttgcccgttt cttcttattt agattgccta taatacagtt
      901 tacatcctct cttgtaagag gctctttgcg gatcacttcc tcccgatttc tgacataaga
      961 gccgttaaat gacagaataa catcaaattc aattccttca aagcgcggta taaacgggta
     1021 gcttcttccc gttgccatac ataaaagaat cccctgcttc tgtaactgtt ttaacgcgga
     1081 tcttaccggt tctgacagat cattgtgcct gaaccggagc agtgttccgt ctatatcaaa
     1141 aaatgcaatt ttaatcataa taactccttc ttttccctat aacaaaagac cggagctctc
     1201 gcccggcctt ctgcctatac gtttctcaat acatccgtaa tttttactcc tgctccagaa
     1261 tcaccttagc aggacacttt tctgcacatt tgccgcaatt agtacacttg tcatagtcaa
     1321 tcacagccag attgttgtcc atatggatgg catctgactc acactgtctg gtacacaggg
     1381 tacagccgat acagccggcc tggcaggctg cctttacatc cttgcccttg tcgtggctgc
     1441 tgcactgtac cagatgcttt gcgctgtaag gcacaagctg gatcagtccc tttggacagg
     1501 cagccacaca tttaccgcag gccacacatt tttccttgtc aaccactgcc acgccgtcaa
     1561 tcacgtggat ggcatcaaac tgacaggcct ttacacagga accaaatccc atacagccgt
     1621 agctgcaggc cttggggctg ccgccgggaa caacggtggc tttggcgcag tcctcgattc
     1681 ctgtataggt gtacagggtt tttgccttat cacaggttcc cttgcagcgt acaaaggcta
     1741 ccttcttatc tgcagcccct gcgtctacac ccaggatgga agcgatcttc tctcctacgg
     1801 gagcgccgcc tacgggacag gcatttgcgg gagcctgacc gtttgcaatg gcctttgcca
     1861 gagcatcaca gcctgcgaag ccgcagccgc cgcagttgtt gcctggaagc tcctcccgga
     1921 tcgccagctc tttttcatcc acttcaacct tgaacttctc gccaaagagg cccaggccga
     1981 taccaactac aatacctacc accgcaatga cagcggcagc cataacaatt gccataatat
     2041 tcatcttcgt ctcctccttc tacagcagtc cggaaaatcc gaaaaatgcg atagccatca
     2101 gtcctgctgt tacaaggaca atgggagatc cctggaagtt atatgggata tcatttccct
     2161 caatacgctc acggataccg gctaacagca cgatggctac ggtatatcca aatgcggttg
     2221 caacaccatt taccacactc tcaatgatgg tgtagtcatt ctgcacattg gttaaagcca
     2281 cgcccagtac ggcgcagttt gtggtaatca gaggcaggaa cacacccaga gcgttataca
     2341 gggataccat gcatttcttt aagaacattt ccacgatctg caccagggct gcgattacca
     2401 gaataaacac aatggttttt aagtaatcaa ggcccagagg ctgcaggatc agcttataca
     2461 gcaggctggc agcaatggat gcgatggtga taacgaagat aaccgcacct cccataccgg
     2521 ctgctgtctc taccttcttg gaaacaccga cgaaggaaca gatgccaagg aactggctta
     2581 acaccacgtt gctgacgaac gcggagctga cggcaattac aagtaattct ttcatctact
     2641 ctttcctcct attcttccgt cttctctttg caggaagcgc agtcgccgct gcagcccttg
     2701 cgtttcttct tatcctttac agagccgttg gtagcacatg gcagctttaa cttattctga
     2761 agtgctgtca ggcaggccag aacaaagaag gcgccgggag ccaggataaa gatagcagca
     2821 ggctcatagg aagcaggcat tacctggatg ccgaaaaccg ttccggcccc taaaagctct
     2881 cttgctccgc cgataaggat cagcgcgatg gtaaagccaa gacccattcc aagaccgtca
     2941 aaggcagaaa tgaggggctt gttcttcatt gcaaaggcct ctgcacggcc caggatgata
     3001 cagtttacaa caatcagggg aatatagatt cccagagcac tgtaaaggga aggggtaaag
     3061 ccttccatca gcatatctac aatggtaacc agagaagcta ccactacgat ctcgcctgga
     3121 agccgcacac gggcgggaat aatatttctc agggcggaga tcacaatatt tgccgcaacc
     3181 agaaccgccg tggtggaaag tcccataccc gcaccgttgg tagcggaggt ggttactgct
     3241 aacgtaggac aaaggcccag catctggatt aagatggggt tttccttcca tacgccgtta
     3301 taaattcgtt ccgtatattt attcatttct tacgcctcct actctccgat gcagttttct
     3361 acaaaataga tggccgcatt tgccgctccg gttactgcat tggatgtata agttgcgccg
     3421 ctgatggc
//
LOCUS       DBMDUH010000210         3367 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_9830_length_3367_cov_7.677587, whole genome shotgun sequence.
ACCESSION   DBMDUH010000210 DBMDUH010000000
VERSION     DBMDUH010000210.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9830_length_3367_cov_7.677587"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1052)
                     /locus_tag="ACZPCG_12570"
     CDS             complement(<1..1052)
                     /locus_tag="ACZPCG_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006779863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HIR1285915.1"
                     /translation="MAKKGKKQTGESPANRSILRITYGIALLFLALILYVGYFIQIQG
                     QEVINNSYNARLDRFSDRVHRGKILASNGTVLAHNEEDEDGNELRVYDHGDVFAHVVG
                     YSTKGKTGIESLANFYLLTSHVNLIEQAVNELSGRKNLGDDVVTTLDINLQQTAYSAL
                     GDRRGAVIAMEPDTGKILAMVSKPDYDPNTLLTDWAALTDSANTQSQLLNRAAQGLYA
                     PGSTFKILTALEYIREHPDNYKDFHFDCNGVYHNGEYSIKCFHSEAHGSQDFVKAFAN
                     SCNGAFSSMGLELDLNRFAATAEELLYNRPLPFSGFPYKESSFNMKAGADIWEILQTS
                     IGQGTTLITPLHSVLIT"
     gene            complement(1052..2410)
                     /locus_tag="ACZPCG_12575"
     CDS             complement(1052..2410)
                     /locus_tag="ACZPCG_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007716693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIR1285916.1"
                     /translation="MVNLILELSKYVMIFLMAVYTWANFRYFSFPDRERKLRLCARQN
                     HVMFFMHFLAYMTLFLKAEDEKTSGLLAAFYAAQAVFFLVYIFLTRRFYRNVSRILVN
                     NTCMLLAVGLIMLSRLSMFRADPLDKALKQFVIICGAAVVSWLIPWIMERFWQIYKFQ
                     WIYAAVGILALLAVWIGGDESFGAQLSFTIGGISIQPSEFVKLTFVLFTASMFYQSTE
                     LKQVCITTAAAAAHVLILVLSKDLGGALILFVSYVLMLFIATGKKLYLFGGIGLGCGA
                     AAAAFGLFDHVKRRVAAWKNPWADIDNTGYQITQSLFAIGTGGWFGMGLCQGMPGKIP
                     VVEKDFIFAAIGEEFGGIFAVCLLLICLGCFIQFMMIASRMQAMFYKLIAFGLGIQYV
                     TQVFLTVGGVTKFIPSTGVTLPFVSYGGSSIVSTFMLFAMIQGLYIIKRNDEEEMGVA
                     ED"
     gene            complement(2414..>3367)
                     /locus_tag="ACZPCG_12580"
     CDS             complement(2414..>3367)
                     /locus_tag="ACZPCG_12580"
                     /inference="COORDINATES: protein motif:HMM:NF013316.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HIR1285917.1"
                     /translation="NQWRRKAPEAGEVQNGADSGEKSSRAAGCAGPVPDESAGKRPMY
                     LTVSAETGDQLSAALAVPEVRRICLDASSFQPERWAEFVQLIHQAGKECYLTLPHIFR
                     THAIGFFRTYRSCLEQAGFDGLLIRAFEEIQWMREEQISLSASFDASVYAWNHGAVHT
                     LKEEQAAFITTPWELNSRELEPVFEACRREGLPAELIVYGRAPMMVSAQCITKTVKGC
                     SKCPSLLWMKDRTGARLPVQNHCAFCYNTILNPLPVSLHGCADSVKRLAPEGLRLCFT
                     IETGEETKAVLNAFAAEFIRGENAEPPFTEFTRGHFRRGVE"
BASE COUNT          892 a          925 c          758 g          792 t
ORIGIN      
        1 gtgatcagga cactgtgcag gggcgtgatc agggtagttc cctgtccgat ggaggtctgc
       61 aaaatctccc agatatcggc tccggctttc atattgaagc tgctctcttt ataaggaaag
      121 ccggaaaatg gcagcggccg gttatataag agttcctctg ccgtagctgc aaaccggttt
      181 aaatccagtt ccagacccat gcttgaaaat gctccgttgc aggaattggc aaaggccttt
      241 acaaaatcct ggctgccatg agcctcgctg tgaaagcatt tgatggaata ctctccattg
      301 tgatatacgc cattgcagtc aaaatgaaag tctttgtaat tgtccggatg ctccctgata
      361 tattccaggg cagttaggat cttaaaggta gaaccgggag catacagccc ctgggccgcg
      421 cggtttaaaa gctggctttg ggtattggcg ctgtctgtga gagcagccca gtccgtaagc
      481 agcgtattgg gatcatagtc cggctttgac accatcgcta aaatctttcc cgtgtccggc
      541 tccatggcga tcaccgctcc tcttcggtct cccaaagcgg aataggcggt ctgctgaaga
      601 ttgatgtcca gagtggttac cacatcatct cccagatttt tccttccgct taattcattt
      661 accgcctgct cgatgagatt tacatgggat gtaaggagat aaaaatttgc cagagactca
      721 attcctgtct ttcccttggt ggaataccct actacatgtg caaatacatc cccatggtcg
      781 taaacccgca gctcatttcc atcctcatcc tcctcattgt gagccagaac cgtaccgtta
      841 gacgccagga tcttccccct gtgaacccgg tcggaaaaac ggtccagacg ggcattgtag
      901 gaattattga tcacctcctg tccttgtatc tgtataaaat accctacata taaaataagt
      961 gccagaaaca gcagggcaat tccataggta atcctgagga tactgcggtt tgcaggagat
     1021 tcccctgtct gtttttttcc tttttttgcc atcagtcctc agccaccccc atctcttctt
     1081 cgtcatttcg tttaataata tacagtccct ggatcatggc aaagagcata aaggtgctca
     1141 caatggaact gccgccatag cttacaaagg gaagagtcac gccggtagac gggataaact
     1201 tcgtcacgcc gcctacggtt aaaaatacct gcgtcacata ctggattccc agcccaaagg
     1261 caatcagctt gtaaaacatg gcctgcatcc gggaagcgat catcataaac tggataaaac
     1321 agcccagaca gattaaaagc aggcagacgg caaagatgcc tccaaactcc tctcctatgg
     1381 ccgcaaagat aaaatccttt tccaccactg ggatttttcc cggcattccc tggcacagcc
     1441 ccatgccaaa ccaccctcct gtaccgatgg caaacagcga ctgggtgatc tgataacccg
     1501 tattgtcaat atccgcccag ggatttttcc aggcagccac tctgcgcttt acatggtcaa
     1561 acaggccaaa ggcagcagcg gccgctccgc agccaagtcc aatcccgcca aacagataca
     1621 gcttctttcc cgtggcgata aaaagcataa gcacatagct tacaaacagg atcaatgctc
     1681 ctcccagatc ctttgacaga accaggatca gcacatgggc cgctgcggcg gcagttgtaa
     1741 tacagacttg tttcaattcc gtggactggt aaaacatgga agccgtaaag agcacaaagg
     1801 tcagctttac aaattccgag ggctggatgg aaattcctcc aatggtaaag gaaagctgcg
     1861 ctccaaagct ctcatcccct ccgatccaga ccgcaagaag agccaggatc cccaccgcgg
     1921 cgtaaatcca ctggaattta taaatctgcc agaaccgctc catgatccac gggatcagcc
     1981 aggaaacaac ggctgccccg cagataatta caaactgctt tagagcctta tctaagggat
     2041 ctgcacgaaa catggacaaa cggctcagca taatcaggcc tacggccaga agcatacagg
     2101 tgttatttac cagaatccgt gaaacattcc ggtaaaaacg ccgcgtaagg aaaatataaa
     2161 ccaggaaaaa cacagcctgg gctgcataaa aggcggctaa aagccccgac gtcttttcat
     2221 cctctgcctt taaaaacaat gtcatatagg ccagaaaatg cataaaaaac attacatggt
     2281 tctgtctggc gcagagcctt agcttccgtt cccgatccgg gaaggaaaaa tagcggaaat
     2341 tcgcccaggt gtagacagcc atgagaaaaa tcatcacata tttggacagt tccaggatca
     2401 gattaaccat accctattct actccccgcc tgaaatggcc tctggtaaat tctgtaaatg
     2461 gaggctctgc attttctcct ctgataaact ctgctgcaaa ggcatttaat actgcctttg
     2521 tctcctcccc tgtttctatt gtaaagcaca ggcgcagtcc ctcaggagcc agacgcttta
     2581 cagaatctgc gcagccgtga agggacacgg gaagaggatt taaaatcgta ttataacaga
     2641 acgcacagtg attctgaacc ggcagccgtg ctccggttct gtccttcatc cacaaaagag
     2701 agggacattt actgcagcct tttacggtct ttgtaatgca ctgggccgat accatcatgg
     2761 gcgcccggcc ataaacaatc agctctgccg gaagcccctc tctgcggcag gcctcaaaaa
     2821 caggctccag ctctctgctg ttcagttccc atggcgtagt gataaaagca gcctgctcct
     2881 ccttcagtgt gtgtactgcc ccatggttcc atgcatagac ggaggcgtca aaggaagcag
     2941 aaagggaaat ctgctcctct ctcatccact gtatttcttc aaaggcacgg attaaaaggc
     3001 cgtcaaaacc tgcctgctcc agacaggaac ggtatgttcg gaaaaatcct atggcatggg
     3061 tcctgaaaat gtgcgggagg gtaagataac actcctttcc ggcctgatgg atcagctgta
     3121 caaattctgc ccagcgctcc ggctgaaagg atgaagcatc cagacagatc ctcctgacct
     3181 ccggcacggc aagcgctgca gacagctgat ccccggtttc tgcggatacc gtaagataca
     3241 tcggtctttt acctgcactc tcatccggga caggcccggc gcatccagcc gcccggctgc
     3301 ttttctcacc ggaatctgca ccgttctgaa cttctcccgc ctccggcgcc tttcttctcc
     3361 actgatt
//
LOCUS       DBMDUH010000211         3343 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_9929_length_3343_cov_5.028069, whole genome shotgun sequence.
ACCESSION   DBMDUH010000211 DBMDUH010000000
VERSION     DBMDUH010000211.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9929_length_3343_cov_5.028069"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            28..363
                     /locus_tag="ACZPCG_12585"
     CDS             28..363
                     /locus_tag="ACZPCG_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285918.1"
                     /translation="MINPYAVMYDARMTVKRWQEVEKDGYTRNELEIVAQDRPCRYSS
                     SGQASIGAPNPSVQNSHKLFCGLEEDVREGDRIVVTLRTEKTVELTLGECHPYTYQWQ
                     CEVKRDDNA"
     gene            360..866
                     /locus_tag="ACZPCG_12590"
     CDS             360..866
                     /locus_tag="ACZPCG_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HIR1285919.1"
                     /translation="MSSSNYRRNEAAIRKFRKELMDMVDDIREIDKRVLNQAVNEGVA
                     YAKRRTPVGAHPNPVVFTIRNGPKVGEVVSFKVSSPGVGGKLRESWHKLPTRRTAGTL
                     ETEMVNNMEYASYWNYGHRIVTKKDGPTKGFVKGTHVLEKTQNYVSKRMIALFKKEVE
                     AVQNRHDQ"
     gene            814..1269
                     /locus_tag="ACZPCG_12595"
     CDS             814..1269
                     /locus_tag="ACZPCG_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006774103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator family protein"
                     /protein_id="HIR1285920.1"
                     /translation="MHYSKRKWRRCRIDTINELYKAVAEGLKDVKKCKVYREDVPQNF
                     TVPCFMVTLYDQNPSRGINGRLKNTVNLDVLYFPENRNQAEQQEECWSVGQELAREFA
                     APGFKIKNRNLKIEDKVLHFMFDVDYREYLEDSTPQMQSIIQNTDVKED"
     gene            1273..2322
                     /locus_tag="ACZPCG_12600"
     CDS             1273..2322
                     /locus_tag="ACZPCG_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002603332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HIR1285921.1"
                     /translation="MAGTWETQDKVLPGAYINIRTNEPLSITPGDRGIAVILQEMFVG
                     EDGQIYTITATEQAYPEKATAEDKKLVAEALKKAKTVLVYKLPETHDADAVNAALKKL
                     KTVQFNTLCYPYDTEPEAASSNKTVITEWIKSMREEEGVKCQAVLANHEADSEGIINV
                     VQGVILTDGTRLTAAETTAWVAGATAGAGITTSNTGMKYMGAVDVSPRMTKTEMETAV
                     KAGKLIFKVDSAQNVTIVSDINSLTTTMPEKGKFFTKNRVIRTLDNLANDISLIFESN
                     YVGQANNTEDGRALLRAGLVDYLTVLQNMAAIQNFETDDVTVKSGNDIDAVVVEVWVQ
                     PVDSVEKIYTTVNLS"
     gene            2335..2814
                     /locus_tag="ACZPCG_12605"
     CDS             2335..2814
                     /locus_tag="ACZPCG_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HIR1285922.1"
                     /translation="MAGKNYTKIKDLVTGSEGSAYITIDGQNRYFFELSKIEANIEFT
                     VIAKKLLGHRMKQHKVVAAEGKGSITMYNVSPVASAIYQQYIKEGKTPTISIQTTNED
                     PASTIGRRVIVMRDCILAKAPVAYLEDGSEDLNTTDSDFTFDDLDDLESYTLPENMR"
     gene            2834..3238
                     /locus_tag="ACZPCG_12610"
     CDS             2834..3238
                     /locus_tag="ACZPCG_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002603335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HIR1285923.1"
                     /translation="MSSLSAFLNPVQAENKEVIVSERFRENGKTVPFVIRPITQQENE
                     TLMKKHRSVDKKSGVEQFNRISYNRELTAMAVVEPDLNNAELQKRYGVLGADKVLSAM
                     LYVGEYGTLMEAVQELSGLDQDINEDMDEAKN"
BASE COUNT         1021 a          642 c          935 g          745 t
ORIGIN      
        1 tgcgccgcca caggaggttg agaggttatg attaacccat atgcagtgat gtatgatgcc
       61 agaatgacag tgaagcgctg gcaggaggta gaaaaggacg ggtatacccg gaatgaattg
      121 gagattgtgg cacaggatcg tccttgccgg tacagctctt ccgggcaggc ctcgatcggc
      181 gccccaaatc cctctgtcca gaacagccat aaattgtttt gtgggctgga ggaggatgtt
      241 cgggagggag accggatcgt ggttactctg cgtacagaaa agacggtaga gcttaccctg
      301 ggagagtgcc atccttatac ttatcagtgg cagtgtgaag taaagaggga tgataatgca
      361 tgagcagcag taattaccgc cggaacgaag ccgccatcag gaagttccga aaggaactta
      421 tggatatggt agatgatatc cgggaaattg acaagagggt gcttaaccag gctgttaatg
      481 agggagttgc ttatgctaaa cgaaggacgc cggtaggagc gcacccgaac ccggtagtct
      541 ttaccatcag gaacggccct aaagtgggtg aggttgtcag ctttaaggtt tctagccctg
      601 gtgtgggagg aaaactccgg gaaagctggc ataagctgcc gaccagacgc actgcaggca
      661 ccctggagac ggagatggtc aataacatgg agtatgcgtc ctattggaat tatggacaca
      721 ggattgttac gaaaaaggac ggcccgacaa aaggttttgt aaaaggaact catgtgcttg
      781 aaaaaacaca gaattatgta tctaaacgca tgattgcatt attcaaaaag gaagtggagg
      841 cggtgcagaa tcgacacgat caatgagtta tataaggcgg tagcagaagg tctgaaagac
      901 gtgaaaaaat gtaaagtata tcgcgaagat gtaccgcaga atttcacggt tccctgcttc
      961 atggtgacat tgtatgacca gaatccttcc cgcggcatca atggacggct gaaaaatacg
     1021 gtgaatctgg atgtcctgta ttttccggag aatcgaaatc aggcagagca gcaggaagaa
     1081 tgctggagcg taggccagga gctggcaaga gaatttgcgg cccctggctt taaaattaag
     1141 aacaggaact tgaaaatcga ggacaaggta ctgcatttta tgtttgatgt agactatcgg
     1201 gaatacctgg aagactccac gccgcagatg cagtctatca ttcaaaatac agatgtaaag
     1261 gaggattaac ctatggcagg tacatgggaa actcaggaca aggtacttcc tggggcatat
     1321 atcaatatcc ggacaaatga acctctgtcc atcacaccgg gggatcgtgg tattgctgtg
     1381 attcttcagg agatgttcgt gggggaggat ggacagatct atacaattac agccacagag
     1441 caggcgtatc cggagaaagc aacggctgag gataagaagc tggttgccga ggcgcttaaa
     1501 aaggccaaaa cagtattggt ctataagttg ccagaaacac atgatgcaga tgcggtcaac
     1561 gcagcactga aaaagctgaa aacggtgcag ttcaatacgc tgtgctatcc ttacgataca
     1621 gagccagagg ccgcatcttc taataagact gtgattacag aatggattaa gtccatgaga
     1681 gaggaggagg gggttaagtg tcaggcggtg ctggcaaacc atgaggcaga ttcagaggga
     1741 atcatcaatg tggtgcaggg ggttattctg acagatggta caaggttaac tgccgcagaa
     1801 actactgcat gggtagccgg agcaacagcc ggggctggta ttaccacttc taataccggc
     1861 atgaagtata tgggggctgt ggacgtatct cccagaatga ccaaaactga aatggaaaca
     1921 gcggtcaagg caggtaagct gatttttaaa gtggacagcg ctcagaatgt tacaattgtt
     1981 tctgacatca actcccttac aaccacgatg ccggaaaagg gaaagttttt tacaaaaaac
     2041 agggttatca gaacattgga taatttggca aatgacattt ctttgatttt tgaaagcaat
     2101 tatgtagggc aagcgaataa taccgaagat ggaagagctc ttcttagagc tggactggta
     2161 gattatttaa cagttttaca gaatatggct gccatccaga attttgaaac agacgatgtc
     2221 acggtgaaat caggaaacga tatcgacgcc gtggtagtag aagtgtgggt acagccggta
     2281 gacagtgtag agaagattta cactactgta aatttatcgt aggaggtgtg agagatggca
     2341 ggaaaaaact acaccaaaat caaagatctt gtgactggca gtgagggcag tgcttatatc
     2401 acaatcgacg gtcagaaccg ctatttcttt gaactgtcca agattgaagc aaatatcgag
     2461 tttacagtga tcgccaagaa gcttttgggc caccggatga agcagcataa ggtagttgca
     2521 gcagaaggaa aaggaagcat caccatgtac aatgtaagcc ctgtggcatc ggctatttat
     2581 cagcagtaca tcaaagaggg gaagacaccc acgatcagca ttcagactac caacgaggat
     2641 ccggcgtcta ctattggtcg acgagtaatc gtgatgaggg actgcattct ggcaaaagca
     2701 ccagtggcgt atctggaaga cggaagtgag gatctgaaca ccactgattc agattttact
     2761 tttgatgatt tggacgatct ggagagctat acacttccgg agaatatgag atagaaagtg
     2821 aggaaaaaag actatgagta gcttaagtgc atttttaaat ccagtacagg cagaaaacaa
     2881 agaggtcatt gtatcagagc gctttcggga gaatggaaag acggttccct ttgtgatccg
     2941 gcctattacc cagcaggaaa atgaaacctt gatgaagaag caccgcagtg tagataaaaa
     3001 aagcggcgtt gagcagttca accgtatcag ttacaaccgt gagctgaccg ctatggccgt
     3061 agtagagccg gatttgaaca atgcagaact gcagaaacgg tatggcgtct taggagcaga
     3121 caaggtattg tccgccatgc tgtatgtggg agagtacgga acactcatgg aggctgtaca
     3181 ggaactgtct ggactcgatc aggatatcaa cgaggatatg gatgaggcaa aaaactgatc
     3241 gagcagggcg atcctgagct atgttacgca cacttcgccc tgcaaagact tcatatcagg
     3301 cctggggtac tggccggtat ctgcggggct aatgatccgg tgg
//
LOCUS       DBMDUH010000212         3321 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_9994_length_3321_cov_4.794410, whole genome shotgun sequence.
ACCESSION   DBMDUH010000212 DBMDUH010000000
VERSION     DBMDUH010000212.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3321)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9994_length_3321_cov_4.794410"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="ACZPCG_12615"
     CDS             complement(<1..356)
                     /locus_tag="ACZPCG_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR1285924.1"
                     /translation="MMTIILGILEEGLVYAIMALGVYITYKILDFPDLSVDGTFPLGA
                     AVTAAGIAGGFLSPVAALFVSFLAGALAGCVTGLIHVKLKVRDLLSGIIVMTALYSIN
                     LRITGKANLPIFSKET"
     gene            complement(368..1453)
                     /locus_tag="ACZPCG_12620"
     CDS             complement(368..1453)
                     /locus_tag="ACZPCG_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003525320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1285925.1"
                     /translation="MRKSMAILLTAAMSASLLAGCGSSSQSSSSAPAEKTTADSSSSS
                     KENADAAPEEGADASYTIGIGQFAEHGSLDNCREGFIQGLAEAGIEEGTNLTILYSNA
                     QADGGTASQIANTFAGKDVDLMCGIATPMAQAQYSLAMKTDIPVIFTAVTDPVAAELA
                     NADGTPVGEITGTSDKLPIEQQLQMIREILPDAKNIGIMYTTSEVNSESAIAEYKELA
                     PKYGFEIIDSGISSSADIPLAADTLIGKVDCITNLTDNTVVASLPVILSKASAKNIPV
                     FGSEIEQVKIGCLAAMGLDYIELGKQTGQMAAKVLKGEAKASEMNYETIKEAAFYGNT
                     EVAENLGITLPESLTSSAVEMFDTIAQ"
     misc_binding    complement(1511..1768)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1916..2428
                     /locus_tag="ACZPCG_12625"
     CDS             1916..2428
                     /locus_tag="ACZPCG_12625"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HIR1285926.1"
                     /translation="MKKLLVVVDMQKDFIDGALGTLEAQQIVPAVRKRICEYREAGDE
                     VVFTLDTHGPDYMDTMEGEKLPVPHCLKGTEGWELDAGLREFDGKRFEKVTFGSPELA
                     EYAAAGGYERIELLGLCTDICVISNALLIKAKTPEAEICVKADCCAGVTPQSHENALN
                     AMKMCHIDVI"
     gene            2451..3089
                     /locus_tag="ACZPCG_12630"
     CDS             2451..3089
                     /locus_tag="ACZPCG_12630"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009536080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HIR1285927.1"
                     /translation="MSSKHFVITVERQYGSGGRLTSERLAKELGIHFYDEEILKMTSE
                     TSAIGEQYFRLADEKAGNNLLYRIVTNIKPELSEPAQNGHKITSPENLFRFQSSVIRK
                     LAASENCIIVGRCGNYVLQDQLDDVIRIFVYADTVTRVRRIMDVDKVDEAEALRRMKR
                     IDRTRTEYHRYFTGRNWMDMENYDLPINASRIDYDQMIVLIKDYMRLRGFLE"
BASE COUNT          877 a          755 c          833 g          856 t
ORIGIN      
        1 atagtttcct tgctgaaaat cggaagattc gcttttcccg tgatcctcag attgatggaa
       61 tagagggctg tcatgacaat aatgccggag agaagatcgc ggaccttaag cttcacatgg
      121 atcaggccgg tgacgcatcc ggccagagcg ccggctaaaa aggatacgaa aagggcggcc
      181 acaggagaga ggaagcctcc tgcaatgcct gcggctgtga cggcagctcc cagaggaaag
      241 gtgccgtcaa cggaaaggtc cgggaaatcc aggatcttat aggtaatgta aacgcccagg
      301 gccataatgg cgtaaaccag gccttcttca agaataccca agatgattgt catgatcgtc
      361 ctccaaatta ttgtgcaata gtgtcaaaca tttcaacggc agagctggtg agcgattcgg
      421 gaagagtgat tcccaggttt tccgctactt ctgtgttgcc gtaaaaagct gcttccttga
      481 tggtttcgta attcatttca ctggcttttg cctcgccctt taataccttt gcagccatct
      541 gtccggtctg ctttccaagc tcaatatagt ccagtcccat agctgccaga cagccgatct
      601 ttacctgttc aatctcactt ccgaatacag ggatattttt ggcggaagcc ttgcttaaga
      661 taacgggaag ggaagcaacc accgtattgt ctgtcagatt agtaatacag tccacctttc
      721 cgataagtgt atcggcagca agcgggatat cagcagagga agagataccg gaatctatga
      781 tctcaaagcc gtattttggc gccagttcct tatactcagc aatggctgat tcggaattta
      841 cctcgctggt ggtgtacatg attccgatgt tctttgcatc gggaagaatt tcacggatca
      901 tctgaagctg ctgttcaatg gggagcttat cactggttcc ggtgatttct cctacaggag
      961 ttccgtccgc atttgccagc tctgctgcaa cgggatcggt tactgcggtg aagataacgg
     1021 ggatatctgt tttcattgcc aggctgtact gtgcctgtgc catgggcgtt gcaatgccgc
     1081 acattaaatc aacatctttc cctgcaaagg tattggcaat ctggctggct gtaccgccgt
     1141 ctgcctgagc attgctgtag aggatggtaa gattggttcc ttcctcaatt cctgcctccg
     1201 ccagtccctg gataaagccc tctcggcagt tatcaagaga gccgtgctct gcaaactgtc
     1261 cgatgccgat ggtgtaggat gcgtctgcgc cttcttccgg agcagcatct gcattttctt
     1321 tggaagagga gctgctgtcc gcggttgttt tttctgccgg ggcagaggat gaactctggg
     1381 aagaggagcc gcagcctgcc aacagggatg cgctcatagc tgctgttaaa aggattgcca
     1441 ttgattttct cataatattt tcctccttga tcatgaaaat aaaaaaacgt cccaattaca
     1501 acctggttgt aattgagacg aatcgaatcc gcggtgccac tcaaattgac tgaaatagtc
     1561 cactctcaca tactatcata tgcgctgcat gcataacggg tgcagaatcc gtcagtccct
     1621 acatcgactg ccgtaaccgg tatgtctgta tcgttcagga ccgccctcga aagtccattc
     1681 agctgccagc ctagtaccgc gatcccacca cccgcggctc tctgtgaaaa tgctttggaa
     1741 gcttactcct ctttctcaat ggtttggctt gttgttttgt atactaatac aggaactgcc
     1801 atttgtcaac tactttttgt gtttttattt ttatattccc caagatgtaa atttgtggta
     1861 ttattatagt acaaaatgaa agtttacaca caagatcatc gggaggagca gaatcgtgaa
     1921 gaaactttta gtggttgtag atatgcagaa ggacttcata gacggagcat tgggtacatt
     1981 ggaagcacag cagattgttc cggctgtaag gaaacggatc tgtgaataca gggaagcagg
     2041 ggatgaggtt gtatttaccc tggacaccca tggtcctgat tacatggata ccatggaggg
     2101 ggaaaagctt cctgttcccc actgtctgaa gggaacagaa ggctgggagc tggatgcagg
     2161 cctcagggag tttgacggaa agcgctttga gaaagttacc tttggaagcc ccgaactggc
     2221 agagtatgca gcagcaggcg gatatgaaag aattgagctt cttggactgt gtaccgatat
     2281 ctgtgtgatc tcaaatgccc tgctgatcaa ggcaaagacg ccagaggcgg aaatctgcgt
     2341 aaaggcggac tgctgtgccg gcgtaactcc acaaagccac gaaaatgctt taaacgctat
     2401 gaaaatgtgt catattgatg tgatctaacc gaaaaaggag gcataaatct atgagcagca
     2461 aacattttgt aattacggta gaacgtcagt acggaagcgg aggacgctta accagcgaac
     2521 gtctggcaaa ggaactggga atccatttct atgatgaaga aatcctgaag atgacctcag
     2581 agaccagcgc catcggggag cagtatttcc gtctggcaga tgaaaaggcg ggaaataacc
     2641 tgctgtaccg cattgtgact aatataaagc cggagctgtc agaacccgcc cagaacggcc
     2701 ataaaatcac ttctccggaa aatctgttcc gtttccagtc ctctgtcatc cgcaagctgg
     2761 ctgcatcaga aaactgcatc attgtgggac gctgcggcaa ctatgtgctt caggatcagt
     2821 tggatgatgt gatccgtatt tttgtatatg cggataccgt taccagagtc cgccgcatta
     2881 tggatgtgga taaggtagac gaggcagagg ccctgcgccg gatgaagcgc attgaccgta
     2941 cccgtaccga gtaccaccgc tactttactg gccgcaactg gatggacatg gaaaactatg
     3001 acctgcccat caacgccagc cgcattgact atgaccagat gatcgtcctg atcaaggact
     3061 atatgagact tcggggattt ttggagtaga cagcatgata tgatagacat tgggagaatc
     3121 tgcctggaga tgggcagatt cttttcaatt tgacagagag tattataagg ctagcaagga
     3181 gataggatta tgaaacgatt ttttgcactc attattgatt taacctaaat tattcacgga
     3241 acagtatctg aattgataac agtaccatga atctgaaaat tgatccatgc agccataaca
     3301 tcactcaatt aacccgtcaa a
//
LOCUS       DBMDUH010000213         3319 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_10002_length_3319_cov_4.670914, whole genome shotgun sequence.
ACCESSION   DBMDUH010000213 DBMDUH010000000
VERSION     DBMDUH010000213.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3319)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10002_length_3319_cov_4.670914"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="ACZPCG_12635"
     CDS             complement(<1..407)
                     /locus_tag="ACZPCG_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HIR1285928.1"
                     /translation="MTKYIFVTGGVVSGLGKGITAASLGRLLKARGLKVAAQKLDPYI
                     NVDPGTMSPYQHGEVYVTEDGAETDLDLGHYERFIDEDLNQYSNLTTGKVYWNVLNKE
                     RRGEYLGSTVQVIPHITNEIKEFVYRVGKKTDA"
     gene            complement(757..2337)
                     /gene="asnB"
                     /locus_tag="ACZPCG_12640"
     CDS             complement(757..2337)
                     /gene="asnB"
                     /locus_tag="ACZPCG_12640"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004065131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HIR1285929.1"
                     /translation="MCSIMGYCSRNAVLSSFMEGFQKTISRGPDDSRVVETGDGILGF
                     HRLAIMGLTPSGMQPFRLGNSYVVCNGEIYGFEKLKKELSVKYTFESNSDCEILLPMY
                     REYGTDMFAMLDAEFACILYDGEAGEFIAARDPIGIRPLYYGYDKDHAIIFASEPKNL
                     TGLTDRIMPFPPGHYYKKGQFICYCDIAKVHRVCYDDLETACGKIHDKLVAGVEKRLI
                     ADAKVGFLLSGGLDSSLVCAIAAKKSSEPIKTFAIGMSEDAIDLKYARQVADYIGSDH
                     TEVYMTPDEVLSSLKTVVQLLGTYDITTIRASIGMYLVCKAIHNQTDIRVLLTGEISD
                     ELFGYKYTDFAPDGKAFQEESQKRIHELHMYDVLRADRCISVNSLEARVPFGDLDFVK
                     YVMALDPELKLNTYGKGKYLLRHAFEQGGYLPDTILWREKAAFSDAVGHSMVDYLKEY
                     AESQYTDEEFKSRCERYTYARPFTKESLLYREIFETFYPGQAEMVVDFWMPNKTWKGC
                     NVNDPSARVLSNYGDSGK"
     gene            complement(2372..>3319)
                     /locus_tag="ACZPCG_12645"
     CDS             complement(2372..>3319)
                     /locus_tag="ACZPCG_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HIR1285930.1"
                     /translation="WIRYKKPDVSMTYNAALAGLVGITAGCDAVSPVGAAVIGIICGI
                     LIVLAVEFFDKIAKIDDPVGAVSVHCVCGATGTILTGLFATGVTTEAGLFYGGGVHLL
                     AVQAAGVVSVAVYVAVVITIVFLAIKYTIGLRADAEDEIAGLDVSEHGLLTAYAGFAM
                     QPDIAAEGEAVPAAVTGDVPAAEAIPVKKVPSFEEGTPKFTKIEIVCRESKFEALKNA
                     MMKLGITGMTVSHVLGCGAQKGKPEYYRGVQVEASLLPKVQVEIVVSAVPVRKVIETA
                     KKVLYTGHIGDGKIFVYDVENVVKVRTGEEGFDALQDVE"
BASE COUNT          838 a          907 c          634 g          940 t
ORIGIN      
        1 tccgcatccg tcttttttcc tacgcggtac acaaattctt taatctcatt ggtaatatga
       61 gggatcacct gaaccgtcga tcccagatat tctcctcgcc gctccttatt cagaacgttc
      121 cagtacacct ttcctgttgt cagattggaa tattgattca gatcttcatc aatgaaccgc
      181 tcataatgtc ccagatccag atcggtttcc gccccgtctt ccgtcacata cacttcccca
      241 tgctgataag ggctcatcgt accgggatct acgttaatat agggatccag cttctgggct
      301 gcaaccttta acccccttgc ctttaaaagc cgtccaagag aagcagctgt aattcctttt
      361 cccaggccgg acaccactcc tccggtcaca aaaatgtatt ttgtcattgt ctttcctcca
      421 tttttgaacg caaaaagggc aaagaggcct ttaagtttat ctaaacttaa agacgccttt
      481 gcctttatgg acgctattct acctcttaag ccctatcttg tcaatccgtt ttttctgaaa
      541 ttcgctcagc cctatcgctt cggatattct gtcccatcag gtctgaaaga gtaatatgat
      601 caacgtagtc atttgccata tggtaaaact ctttccacat atctccttat cctattgtac
      661 tgcgtgacgc tccagccaca gggacagggt ttcgaatctg tattttaata ataattacag
      721 aactttagcg ataatacaga aaaaagagtg gttattttat tttccgctgt ctccgtagtt
      781 agaaagcaca cgcgcagatg ggtcatttac gttacatccc ttccacgttt tgtttggcat
      841 ccaaaaatca actaccatct ctgcctgtcc cggatagaaa gtttcaaaaa tctccctgta
      901 taataatgac tcttttgtaa aaggacgggc atacgtataa cgttcacatc tgcttttaaa
      961 ttcttcatcc gtatattggc tttctgcata ttccttcaga taatcaacca tggaatggcc
     1021 tactgcatct gaaaaggcag ctttctctct ccaaaggatt gtgtccggaa gataaccgcc
     1081 ctgttcgaag gcatggcgga gcagatattt tccctttccg taggtattca gtttcaattc
     1141 cggatccagc gccatcacat actttacgaa atcaagatct ccaaaaggaa cccttgcctc
     1201 cagggagttt acggaaatac agcggtctgc acgcagtaca tcgtacatat gcagttcatg
     1261 gatccgtttc tgggattctt cctgaaaagc tttcccgtcc ggtgcaaagt ccgtatattt
     1321 atagccgaac agctcgtctg agatctctcc agttaaaaga actcggatat ctgtctggtt
     1381 atggattgcc ttacacacta gatacattcc tatggatgcg cggattgtgg ttatatcata
     1441 ggttcctaac agctgaacca cagtcttcag agaggacagt acctcatccg gcgtcatgta
     1501 aacctctgta tggtcgcttc ctatataatc tgccacctgc cttgcatatt ttaagtcgat
     1561 ggcatcttcg ctcattccga tggcaaaggt cttgatcggc tcgctgcttt tctttgctgc
     1621 aatcgcacac accaaggacg aatccaggcc gccggaaagc aggaatccca cctttgcatc
     1681 tgctatcaga cgtttctcta cgcctgccac cagcttatca tgaatcttcc cgcaggcagt
     1741 ttccagatcg tcataacaga ccctatggac tttggctata tcgcagtaac agataaactg
     1801 tcccttttta taataatgtc ccggaggaaa cggcatgatc ctgtccgtaa gccctgtcag
     1861 atttttgggt tcactggcaa atatgatcgc atgatcttta tcatatccat aatacagtgg
     1921 gcggattcca atgggatctc ttgctgcgat aaactcccct gcttccccgt cgtaaaggat
     1981 gcaggcaaat tcggcatcca gcatggcaaa catatctgtg ccgtattccc gatacattgg
     2041 cagcagaatt tcacaatccg aattgctttc aaacgtatat tttactgaca gctctttttt
     2101 cagcttctca aatccataaa tttctccatt acagactaca taactgtttc cgagccggaa
     2161 gggctgcatt cccgacggag tcaggcccat aatggctaat cggtggaatc caagaattcc
     2221 atcgcccgtt tcaaccactc tgctgtcatc cggccccctt gatatggttt tttgaaatcc
     2281 ttccataaaa ctgctcagaa ccgcattgcg gctgcaataa cccatgatgg aacacatagg
     2341 aacatacctc caaaatctta agtttccttt tttattctac gtcctgcagg gcgtcaaagc
     2401 cctcttctcc ggttcttacc tttactacat tttccacatc ataaacaaaa atcttgccgt
     2461 ctccaatatg gccggtataa agaaccttct ttgccgtttc aatcacctta cgaaccggaa
     2521 ccgcactgac tacgatctcc acctgtacct tcgggagcag gctggcttct acctgtactc
     2581 cacggtaata ctccggtttc cctttctgtg cgccgcagcc taatacatgt gatacggtca
     2641 taccggtaat accgagtttc atcattgcat tcttcagcgc ttcaaatttg gattccctgc
     2701 atacgatctc aattttcgta aatttagggg ttccttcttc aaaagacggc acttttttaa
     2761 ccggaatcgc ttctgcagcc ggaacatctc cggttactgc agccggaaca gcctcaccct
     2821 ctgccgcaat atccggctgc attgcaaagc ctgcataggc ggttaaaaga ccgtgttctg
     2881 atacgtccag tccggcaatc tcatcctctg cgtcggcccg gagcccaatg gtatatttga
     2941 tcgccagaaa tacaatcgta ataacaaccg ccacatatac cgccacagag acgacgcctg
     3001 ctgcctgaac tgccagcaga tggactccgc cgccataaaa aagacctgct tctgtcgtta
     3061 cgcctgttgc aaacaggccg gtgagtatgg ttcctgtcgc gccgcacacg cagtgaacgg
     3121 acacagcgcc cacagggtcg tcaattttgg cgatcttatc aaaaaactct actgccagaa
     3181 caataaggat tccgcatatg atcccaataa cagctgcccc aaccggactt accgcgtcac
     3241 atccggccgt aattccgaca agacctgcca gcgccgcgtt atacgtcatg gaaacatccg
     3301 gctttttata acggatcca
//
LOCUS       DBMDUH010000214         3311 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_10031_length_3311_cov_3.783178, whole genome shotgun sequence.
ACCESSION   DBMDUH010000214 DBMDUH010000000
VERSION     DBMDUH010000214.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10031_length_3311_cov_3.783178"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="ACZPCG_12650"
     CDS             complement(<1..572)
                     /locus_tag="ACZPCG_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006864339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HIR1285931.1"
                     /translation="MDGADAAIRSGEMDAIKQISYTIKDIYNLPEGKRAELINGQIYM
                     MAPPSTKHQKLISALHGKIYNYIESHNGNCEIIPAPFAVFLKDNDENESYVEPDISVI
                     CDPDKIDDKGCHGSPDWIIEIVSSSSRKMDYSRKQFLYLEAGVLEYWIVDPIKETILI
                     YRQSEDWVPLFARFGEKVKSGIYDDLEIVI"
     gene            complement(893..2281)
                     /gene="radA"
                     /locus_tag="ACZPCG_12655"
     CDS             complement(893..2281)
                     /gene="radA"
                     /locus_tag="ACZPCG_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HIR1285932.1"
                     /translation="MAKAKTTAFFCSECGYESSKWMGQCPACKAWNTLVEEPTAGSAR
                     SASPSGRLSAVSGIGAGKREKAALLSEISLEEEDRISTGFKELDRVLGDGIVAGSLIL
                     VGGDPGIGKSTLLLQVCRQLAADGKKVLYISGEESLKQIKMRACRIGSVTGDLRFLCE
                     TNLDRISQAIDLEKPQIVVIDSIQTMYREEISSAPGSVSQVKESTGLLMQIAKSRGIA
                     IFVVGHVTKEGVVAGPRVLEHMVDTVLYFEGDRNAVYRVLRSVKNRFGSTNEIGVFEM
                     REEGLMEVENPSEYMLDGRPENASGAVVSCSLEGSRPILLEVQALVAETNFGMPRRAA
                     AGTDYNRVNLLMAVLEKRCRYEMSRLDAYVNIAGGMRMNEPALDLAIVMALMSSYKDR
                     PVDPGMLIFGEVGLSGEVRAVSQADKRAAEAARMGFTSCVIPKVCLDKMKPIKGLKMI
                     GVSNVREAIGLL"
     gene            complement(2387..2803)
                     /locus_tag="ACZPCG_12660"
     CDS             complement(2387..2803)
                     /locus_tag="ACZPCG_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endosialidase"
                     /protein_id="HIR1285933.1"
                     /translation="MPVVEELIRTEQDGTISFGNYTLGQKAKKSDFEYQGDVYKVKTF
                     YEITKLERNDLFVYESVPGTVAEHFLVSDEGVEFSVEGAKDAQITVQLEDDTDYEVYV
                     DGVAVGSMKTNMSGKLSVSVELEEGTAVKVKAVKRA"
     gene            complement(2932..>3311)
                     /locus_tag="ACZPCG_12665"
     CDS             complement(2932..>3311)
                     /locus_tag="ACZPCG_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpC"
                     /protein_id="HIR1285934.1"
                     /translation="EIIVFHQLNREHMKGIADIMLQGIIKRSREQLGITLSVDTAARD
                     YLIDKGYDDKYGARPLRRTIQNLLEDKLAEAVLDHKVRKNALVEVGLEGEDLSFSVKK
                     KAVPRKKSASGEIEKQVLENKAK"
BASE COUNT          828 a          936 c          587 g          960 t
ORIGIN      
        1 tcgattacta tttccagatc atcataaatt ccggacttta ctttctcgcc aaacctggca
       61 aataacggaa cccaatcctc agattgcctg taaattaaaa tagtttcttt tataggatca
      121 acaatccaat attccaggac acctgcttcc aaatataaaa attgctttct ggaatagtcc
      181 attttcctgc ttgaagatga aactatttct ataatccagt ccggcgatcc atgacagcct
      241 ttatcatcta tcttatcagg atcacagatt acagatatat ccggctctac ataggattca
      301 ttttcatcat tgtcttttag aaacacagca aatggcgcag gaataatttc acagtttcca
      361 ttatgtgatt caatataatt ataaattttc ccatgcaaag cacttataag tttttgatgt
      421 tttgtgcttg gtggagccat catataaatc tgtccgttaa ttaactctgc ccgtttaccc
      481 tctggaagat tgtagatatc ctttattgta taggatattt gttttattgc gtccatttca
      541 ccagaacgaa ttgccgcatc tgcgccatcc atcaaacaca acgtaccctc tccgataatg
      601 agcatcagat tgtgctaata ctcagctcat tcattccaag cctccaatcg ggaaattttc
      661 tatatttata ctaatctgat tcttcccatc ggacatcttc ttccattcct ttctgataaa
      721 catagtttac cagaacaaaa ccaaaaagaa aaccatcctt taaaataggt tcgtcaaaat
      781 aacgttggat tattttgtgt attttgggga tctgtaaaat gtcggttagc aacgtatgaa
      841 aaagaagtct gaatcagaat ttcgttcgaa cgttatccat atgatttcac ccctacaaaa
      901 gcccgatcgc ctcccgcaca ttggacaccc cgatcatctt aagccctttt atgggcttca
      961 tcttatcgag acaaaccttt ggaatcacac aggaagtaaa gcccatcctg gctgcctcgg
     1021 ctgcccgttt gtccgcctgt gaaaccgccc gaacctcccc ggaaagcccc acttctccaa
     1081 atataagcat acccggatcc accggccggt ccttatagct ggacataaga gccattacaa
     1141 tggcaagatc taacgccggc tcattcatcc gcataccgcc tgcgatattg acataggcgt
     1201 ccagacggga catctcgtat cggcaccgtt tctcaagcac tgccatgaga agattcacac
     1261 ggttatagtc cgttcctgcc gccgcgcgtc ttggcatgcc gaaatttgtt tcagctacca
     1321 gagcctgaac ctctaaaagg atgggacggc ttccttccaa agagcaggaa accactgccc
     1381 cggaagcatt ttccggccgg ccgtccaaca tatattctga aggattctcc acctccatga
     1441 ggccttcctc ccgcatctca aatacaccga tttcattggt agaaccaaaa cggttcttta
     1501 cacttcttaa aacccgatag actgcgttcc ggtctccttc aaaatagagc accgtatcca
     1561 ccatatgctc caacactcta gggcctgcca ccaccccctc ctttgtcaca tggcctacta
     1621 caaagatggc aatgccccgg ctctttgcaa tctgcatcaa aaggcctgtg gattctttta
     1681 cctggctgac gcttccggga gcagaggaaa tctcctcccg gtacatagtc tggatggaat
     1741 cgatcaccac aatctggggc ttttccagat caatggcctg actgatccgg tccagatttg
     1801 tctcgcacaa aaaacgcaga tctcctgtta cgcttccgat ccggcaggcc cgcatcttga
     1861 tctgctttaa ggactcttct cctgatatat agagcacttt ttttccatct gctgccagct
     1921 gacggcagac ctgtaataaa agagtggatt tcccgattcc cggatctcct cccaccagta
     1981 tcagagaacc ggctacgatc ccgtctccca gcacacgatc cagctcctta aagcctgtgg
     2041 aaatccgatc ctcttcttct aaggatatct ctgataagag ggcagctttc tccctcttgc
     2101 ctgcaccgat cccgcttacg gcagacagac ggccagaggg ggatgcagac cgtgctgatc
     2161 ctgccgtcgg ttcctccacc aaggtattcc atgccttaca agccggacac tgacccatcc
     2221 atttggaaga ttcatatccg cattcactgc agaaaaatgc agttgtcttt gcttttgcca
     2281 tttgtttact ccctgttctt tgatccgctg acagcggaat tctgaaaaaa ggaggccgtc
     2341 tccggcctcc taatgttccg tctgttttct cagacagaat ccatccttac gctctcttta
     2401 cggcctttac ctttacagcc gttccctctt ccaactctac actgacactg agctttccgc
     2461 tcatgttggt cttcatgctg cctacagcca ctccgtctac atacacctcg tagtctgtgt
     2521 catcctccag ctgtacagtg atctgggcat ccttagcgcc ctctaccgaa aactccacac
     2581 cttcatctga cacaaggaaa tgttctgcaa ctgtacccgg aacggattca tatacaaaca
     2641 gatcattgcg ctccagcttt gtgatctcat agaaggtctt taccttgtaa acatctccct
     2701 gatattcaaa atcggacttc ttcgctttct gtcccaatgt gtaattacca aaactgatgg
     2761 tgccatcctg ctctgtgcgg atcagttctt caacaaccgg catcttttta tcctcctgtt
     2821 cattcagtcc cgtcccataa ggcggactgc cattctccac tccctgtctc acagggatat
     2881 tctgtactcc gattatacaa caaccggttc aaaataacca gttatttttt cttactttgc
     2941 cttattttct aaaacctgct tttctatttc gccggaagcg gactttttcc gcggtacagc
     3001 tttctttttt acggaaaagc tcagatcctc tccctcaagt cctacctcta ccagagcatt
     3061 ttttcgcacc ttatgatcca gcacagcctc tgccagctta tcctcaagaa ggttctggat
     3121 cgtacgtctt aaaggccttg cgccgtattt gtcatcatag cccttatcga tcagataatc
     3181 tctggccgcc gtatctacag acagagtaat ccccagctgt tctctgctgc gctttataat
     3241 accctgcagc ataatatctg caatcccctt catatgttct ctgtttaact ggtggaacac
     3301 gatgatctca t
//
LOCUS       DBMDUH010000215         3308 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_10042_length_3308_cov_6.835672, whole genome shotgun sequence.
ACCESSION   DBMDUH010000215 DBMDUH010000000
VERSION     DBMDUH010000215.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10042_length_3308_cov_6.835672"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..151
                     /locus_tag="ACZPCG_12670"
     CDS             <1..151
                     /locus_tag="ACZPCG_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HIR1285935.1"
                     /translation="VWIGGGAIIMPGVTIGNSIVIGAGSVVTKDIPDNVIAYGNPCKV
                     VRENK"
     gene            complement(521..1177)
                     /locus_tag="ACZPCG_12675"
     CDS             complement(521..1177)
                     /locus_tag="ACZPCG_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HIR1285936.1"
                     /translation="MHTGHIQLEQVLPAEIENRSFEIIQDELAAMGKVLDPVQDLLIR
                     RAIHTTADFDYADNLVFSEGAVEAGLNALREGAVIITDTNMAWSGINKKKLANLGGEA
                     VCFMADESVAARAAASGSTRAVASMEKAAELYGNASRPCIFAIGNAPTALVRLYELVQ
                     EGRISPALIIGAPVGFVNVVPSKELILTLPRTPYIVARGRKGGSNVAAALCNALLYQV
                     "
     gene            complement(1213..2787)
                     /locus_tag="ACZPCG_12680"
     CDS             complement(1213..2787)
                     /locus_tag="ACZPCG_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HIR1285937.1"
                     /translation="MAKCIMIQGTMSNAGKSLLAAGLCRIFAQDGYRVAPFKSQNMAL
                     NSYITADGLEMGRAQAVQAEAAGTEPRADMNPILLKPTTDIGSQVIVNGISRGNMRAK
                     DYFAYKKELIPEILAAYNRLCGDYDIIVIEGAGSPAEINLKKDDIVNMGLAAMVDAPV
                     LLAGDIDRGGVFAQLYGTVALLEESERKRICGLIVNKFRGDSSILEPGIRQLEELCGI
                     PVAGVVPYMDVDIEDEDSLSEKLGQREKGLVDLAVIRFPKISNFTDLDVFVRIPGVTV
                     RYVTKVSELGTPDLVILPGTKNTISDLLWMRQNGLEGAILKLAGRNVPVWGICGGYQM
                     MGEEVIDEEGAESGECRRIETMGLLPLRTVFEKEKIRTRSEGSLAELDGVFAPLSGKC
                     IFGYEIHMGRTEIACPEGPAGTARLVPDRKEPEICRPMCYLFKGDTKQVQPDGWNRGN
                     CYGAYVHGIFDAPGVADTLVNALAEKKGIKLETNLKEEYDAYRNRQYDKLAGELRKAL
                     NMELIYKVLGLEGRKE"
     gene            complement(2819..>3308)
                     /locus_tag="ACZPCG_12685"
     CDS             complement(2819..>3308)
                     /locus_tag="ACZPCG_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR1285938.1"
                     /translation="IPKLKDLDHVIVLKAFTKIYAMAGLRLGYSLCADQELNIRLETV
                     RQPWSVSGPAQRAGIAALKERDYLEATKRLIGPERERMKGKLVELGFQVYPSQANYIF
                     FRDPVWKEGDSLYDRLLRQGVLIRNCGNYPGLDGSYYRICVKTAEENDCFLRYLTEAV
                     GR"
BASE COUNT          794 a          963 c          686 g          865 t
ORIGIN      
        1 tgtatggatt ggtggcggag ctattatcat gcctggtgtt acaatcggaa atagtattgt
       61 cattggagca ggaagtgttg tgacaaagga cattcctgat aatgttatag cttatgggaa
      121 tccctgtaaa gtagtaagag agaataaata aacttccagt ttatcagtga attaagcgaa
      181 acagtaacct gcaccgatta caaagacaag atttgaaaat tgatatgtca tatgcagaaa
      241 agcactgaaa aaaagatccc acatggctgc attccggatt cggaccggac ccttccgggc
      301 tacgaacccg gcgtccccag gacccttgtc catgtggcga tctgtttcta tcttaacata
      361 atcgatacct atttacaatc gcttctctca gaccattttt ttcatatctg ccaccagggc
      421 aaactgtgtt ctgcagcgat ccagattgtg attttcacgg tgaatcttcc tgctcctccc
      481 aatctctttc cggatccaat ttccgttttt tatcccctcc tcacacctga tacagcagcg
      541 cattgcacag cgcagccgcc acgttgctcc cgccttttct tcccctggct acaatataag
      601 gcgtacgggg aagcgtcaaa atcagttcct ttgagggaac cacatttaca aatcccaccg
      661 gagcgccgat gataagggcg ggagaaatcc tcccctcctg caccagctca tagagacgca
      721 ccagggccgt aggggcattt ccaatggcga agatacaggg acgggaagca tttccataaa
      781 gctctgccgc cttttccatg ctggcaacag ccctggtgga accggatgcg gctgcccggg
      841 ctgctacgga ctcatctgcc ataaagcaca ctgcttctcc tccaaggttt gccagctttt
      901 tcttattgat tcccgaccag gccatatttg tgtccgtaat gatcacagcc ccctcccgca
      961 gtgcgttcag ccctgcttcc acggctcctt cggaaaatac cagattgtcc gcatagtcaa
     1021 aatctgccgt agtatggatc gccctgcgga tcaaaagatc ctgcaccgga tccaggacct
     1081 ttcccatggc agccagctca tcctgaatga tctcaaagct tctgttctca atctcagccg
     1141 gaagtacctg ctccagctgg atatgtcctg tatgcatctg atattctgta tttcttttat
     1201 cttccaaaaa cctcactcct tccggccctc aagtcctaaa accttataaa tcaattccat
     1261 atttaaagcc tttctcagtt ccccggccag cttgtcatac tggcggtttc ggtaggcgtc
     1321 atactcttct ttcagatttg tttccagctt aatgcccttt ttctcagcca gagcatttac
     1381 cagcgtatcc gccacacccg gagcatcaaa tatgccatgg acataagctc cgtaacagtt
     1441 tcctctattc cagccatccg gctgcacctg ctttgtatcc cctttaaaaa gatagcacat
     1501 gggacggcag atttccggct ctttcctgtc cggaaccagc cttgcggttc cggcaggccc
     1561 ctccggacat gcaatttccg tccgccccat atgaatctca taaccaaata tgcatttgcc
     1621 ggacaagggc gcaaacacac cgtccagctc tgccagcgag ccctctgagc gggtacggat
     1681 tttttctttt tcaaaaaccg tccgcagagg aagaaggccc atagtttcaa tccggcggca
     1741 ttccccggat tcggctcctt cctcatcgat cacctcttcc cccatcatct ggtatcctcc
     1801 gcagattccc catacaggca cattccgtcc ggccagttta agaatggctc cttccagacc
     1861 attctggcgc atccacaaaa gatcgcttat ggtatttttc gttccgggaa ggatcaccag
     1921 atccggtgtg ccaagctcgg acaccttcgt cacataccgc accgtcacgc cgggaatcct
     1981 cacaaataca tccagatctg taaaattgga aattttaggg aatcggatga ccgccagatc
     2041 caccagtccc ttttcccttt gaccaagctt ttcgctcagg ctgtcctcat cctcaatatc
     2101 tacatccata tagggaacta ctcccgctac cgggataccg caaagctcct ccaactgccg
     2161 aatccccggc tctaaaatag aggagtctcc cctgaattta ttcacgatca ggccgcagat
     2221 ccgttttctt tcactttctt ccaaaagagc cacggttccg taaagctgcg caaatacgcc
     2281 gccccggtca atatctcctg ccaaaagcac cggagcatcc accatggcag ccagacccat
     2341 atttacaata tcatcctttt ttaaattgat ctctgccgga cttcctgctc cctctatgac
     2401 aatgatatca taatccccgc acagacggtt ataagccgcc aaaatctccg gaatcagctc
     2461 ttttttatag gcaaagtaat ccttcgcccg catattccct ctggaaatac cgtttacgat
     2521 cacctgagag cctatgtctg tggtgggttt taaaaggatg gggttcatat ctgcccgcgg
     2581 ctctgtccca gccgcctctg cctgaaccgc ctgagcccgc cccatttcca gaccgtctgc
     2641 ggtgatatag gagttaagag ccatattttg ggatttaaag ggagcgaccc ggtagccgtc
     2701 ctgggcaaaa atccggcaga ggccggcagc caaaaggctt tttcccgcat tggacatggt
     2761 tccctgtatc atgatgcatt ttgccattat tcttctcctt atagcgtttt gagggaaatc
     2821 atcttcccac agcctctgta agatatcgga ggaaacagtc attttcttcc gctgtcttta
     2881 cgcagatccg gtaataagag ccgtctaatc cgggataatt gccgcagttt ctgatcagca
     2941 cgccctggcg cagcagtctg tcataaaggg aatccccttc cttccagaca ggatctctga
     3001 aaaaaatata attggcctgg gagggataaa cctgaaagcc aagctctaca agcttcccct
     3061 tcatccgttc ccgctccggc ccaatgagcc tttttgtagc ttccagataa tccctttctt
     3121 taagtgctgc aataccggcc ctctgagccg gtccggaaac agaccagggc tgacggactg
     3181 tttccaggcg gatgtttaat tcctgatctg cacagaggct gtagcccagc cgcagcccgg
     3241 ccatggcata aattttggta aatgccttta agacaatcac atgatccaga tcctttaatt
     3301 tagggatt
//
LOCUS       DBMDUH010000216         3276 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_10155_length_3276_cov_4.222362, whole genome shotgun sequence.
ACCESSION   DBMDUH010000216 DBMDUH010000000
VERSION     DBMDUH010000216.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10155_length_3276_cov_4.222362"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(165..695)
                     /locus_tag="ACZPCG_12690"
     CDS             complement(165..695)
                     /locus_tag="ACZPCG_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007887978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIR1285939.1"
                     /translation="MTERFSTKNMVTAAVFLALGMVLPFFTGQIPTIGSMLLPMHIPV
                     LICGYVCGWQWGLLVGLIVPILRSMTLGMPPMMPVAAAMAFELAAYGASTGLLYEKLE
                     KNTISIYISLIGAMIFGRIVWGLVSMVLYGIMGNAFSVQIFLSGALLKAVPGIILQLV
                     LIPVIIITLRKAKVIE"
     gene            complement(1001..2440)
                     /locus_tag="ACZPCG_12695"
     CDS             complement(1001..2440)
                     /locus_tag="ACZPCG_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008725613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HIR1285940.1"
                     /translation="MNRSIIIYMLGWIMNIEAVLLFLPIITAAVYRESVITFYLVVSC
                     ICGVLGFLCTRKKPKVKMFFAKEGFVLVSLGWIVLSFFGCMPFWLSGEIPHFIDALFE
                     IVSGFTTTGASIVPKVEELSKATLMWRSFSHWIGGMGILVFILSILPMTGDYNMHIMR
                     AESPGPSVGKLVPKIRITAKLLYQIYFFMTIATMGFLLIGGMPVFDTICMSLGAAGTG
                     GFACRNSGQADYTLYQQAVLSIAMILFGVNFNVYYLLLIKKPKDAFRCEEMRGYLAVI
                     LGAVILITLNVRHLFPSLFLAVHHVLFQVASIITTTGYATVDFNQWPEFSKCIVLLLM
                     FIGACAGSTGGGIKVSRIMMAFKEAKKEMAAVIHPRSVKVIKYEGKVLGHPVLRSLNA
                     YLILYALIFAVSVLLVSLDNFDFATSFSAVAANFNNIGPGLSTVGPSSNFSMLSDLSK
                     LVLIFDMLAGRLELFPILVLFSPGTWKKG"
     gene            complement(2454..>3276)
                     /locus_tag="ACZPCG_12700"
     CDS             complement(2454..>3276)
                     /locus_tag="ACZPCG_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007718125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HIR1285941.1"
                     /translation="LICVVERGNDVVIPDGNFVMKKGDKISFIASHQGSADFFKKAGV
                     DNNIVKSAMFVGGGKLTHYLCRLLEDTKIKIKIIERDEERCRQLSELLPKAMIIHGDG
                     TDEQLLLEEGIRQTEAFASLTGFDEENIMLSLYASSQSKAKLITKVNKIAFENVINSL
                     NIGSLIQPKMLTAEIILQYVRAMQNSMGSSNIETLYKIAADKAEALEFRVKEGSPILG
                     IPLEKLKLIDNLLVACINRGGTIITPRGKDTVEAGDTVIVVTTHTGLNDLTDILR"
BASE COUNT          951 a          789 c          689 g          847 t
ORIGIN      
        1 cacttaaagc ttttgtactc ttctttcaga cgatccaggc ttttttcagg actggtgatc
       61 atatggccgc cgccaaattc gctctgatac agcagcttta ccacatcctg gagctgcatc
      121 tggggatatt gttttaaatg cgcaagcagt atttttttca tattttattc gattaccttt
      181 gcttttctca atgttataat aataacagga atcaggacaa gctgcaagat aattcccggg
      241 actgccttta aaagggctcc gcttaaaaaa atctgcacag aaaaggcatt tcccataatt
      301 ccataaagga ccatgcttac aagtccccat acgatacgcc cgaaaatcat ggcaccgatc
      361 agactgatat aaatggaaat ggtatttttc tccagttttt catagagaag ccctgtagaa
      421 gctccgtaag ctgcaagttc aaatgccata gcagctgcta cgggcatcat aggaggcatt
      481 ccaagagtca tgctgcgcag aatgggaaca atcaggccga ctaaaagccc ccactgccag
      541 ccgcagacat agccgcagat caaaaccgga atgtgcatgg gaagaagcat acttccaata
      601 gttggaatct gaccggtgaa gaagggaagc accatgccaa gggccaaaaa tacagctgct
      661 gttaccatgt tttttgttga aaatcgttct gtcatacctt actctccttt ttgcacaaaa
      721 aataccagaa atgtacctat cctgtcggat ttttgtacct tcctggtatc aattacgcat
      781 gtttattttt aaatcttccg cttttgcggg aacggcttca gccgggaacg ctggcttgtg
      841 ccagctgctt tatttattat aacggacaag gggcactacg tcaagaaaat atgaaaatcc
      901 aaggatggcg ttattttacg atgttttgcg ataaaagaag gataacataa aaacagcgcc
      961 tgctcccttc acattcctga gaacagacgc tgtctttcag ttatcctttc ttccacgttc
     1021 ccggcgaaaa cagcaccagg atcgggaaaa gctccagccg tccggccagc atatcaaaga
     1081 tcaataccag ctttgataaa tcagacagca tggaaaaatt agagcttggt ccgacggtcg
     1141 ataaaccggg cccaatgttg ttaaagtttg cagccaccgc agaaaaactt gtggcaaaat
     1201 caaagttatc cagactgacc agcagcacag acactgcaaa aatcagggca taaagaatca
     1261 gatatgcatt cagcgaacgc aggacggggt gtcccagcac ttttccctcg tatttaatca
     1321 ccttgacgct gcggggatgg atcactgctg ccatttcctt tttggcctcc ttgaaggcca
     1381 tcataatgcg tgataccttg atgccgcctc cggtgcttcc tgcacaggcg ccgataaaca
     1441 tcaaaagcag aacgatgcat ttggaaaatt ccggccactg gttaaaatcc actgtcgcat
     1501 agcctgtagt tgtgataata gatgctacct ggaacagaac atggtgaact gccagaaaca
     1561 gggatggaaa caggtggcgt acattcagag taatcaggat tactgctccc agaatgacgg
     1621 ccagatagcc gcgcatttct tcacagcgga aggcatcctt gggttttttg atcaaaagca
     1681 ggtaataaac attaaaattc acgccaaaaa ggatcattgc gatacttaaa acggcctgct
     1741 gatacaaggt ataatctgcc tggccgctgt ttctgcaggc aaaaccgccg gttcctgctg
     1801 caccgagact catgcagatg gtatcaaata caggcattcc gccaatgagc agaaagccca
     1861 tggtagcaat ggtcataaaa aagtagatct ggtacagaag ctttgctgta atgcggattt
     1921 ttggcaccag ctttcctaca gaagggccgg ggctttctgc acgcatgata tgcatattgt
     1981 aatccccggt catgggcagg atggaaagga taaagactaa aatccccata ccgccgatcc
     2041 agtgggaaaa gctgcgccac atcaaagtag ccttggaaag ctcttctacc tttggcacaa
     2101 tactggctcc ggtagttgta aaaccggaaa caatttcaaa cagcgcatca ataaaatgag
     2161 gaatttcgcc gctgagccaa aagggcatac agccaaaaaa gctgagtacg atccagccta
     2221 aggacaccag tacaaagcct tcttttgcaa aaaacatttt tactttgggc tttttcctgg
     2281 tacacaaaaa gcccaaaact ccgcagatgc aggaaacaac cagatagaag gtgattacgg
     2341 attcccggta aacggctgcc gtaataatag gcagaaacag caagacggct tcaatgttca
     2401 taatccagcc aagcatatag ataataatac ttcgattcat ctctctcccg cctttacctt
     2461 aaaatatctg taagatcgtt taatcctgta tgagtcgtaa cgacaataac cgtatctcca
     2521 gcttcaaccg tatctttgcc tcgtggagtg atgatcgttc cgccccggtt gatgcaggcc
     2581 actaaaagat tatcgatcag cttcagtttt tccaaaggaa tgccaagaat cggcgatccc
     2641 tcctttacgc gaaattccag cgcctccgct ttatctgcgg caattttata aagagtctca
     2701 atattgctgc ttcccatgga attctgcata gcacggacat actggaggat aatctcagca
     2761 gtgagcatct tgggctggat cagacttccg atatttaaag aattaataac attttcaaaa
     2821 gcaatcttat tgacttttgt gataagtttt gctttcgact ggctggaggc atagagggac
     2881 agcatgatat tttcctcatc aaatccggta agactggcaa acgcctctgt ctgtcggatc
     2941 ccttcctcca aaagaagctg ttcatccgta ccgtcgccgt ggatgatcat agctttggga
     3001 agaagttcgc ttaactggcg gcacctctct tcgtctcttt cgatgatttt aatcttgatt
     3061 tttgtgtcct ccagcagcct gcacagataa tgggtcagct ttccgccgcc cacaaacatg
     3121 gcgcttttga caatattatt atcaactccc gcttttttaa agaaatccgc acttccctga
     3181 tgagaggcaa taaaggaaat tttatctccc tttttcatca caaaattacc gtccggtatc
     3241 acaacgtcat ttccgcgctc caccacacag atcaga
//
LOCUS       DBMDUH010000217         3219 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_10381_length_3219_cov_5.663887, whole genome shotgun sequence.
ACCESSION   DBMDUH010000217 DBMDUH010000000
VERSION     DBMDUH010000217.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10381_length_3219_cov_5.663887"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="ACZPCG_12705"
     CDS             complement(<1..688)
                     /locus_tag="ACZPCG_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285942.1"
                     /translation="MENFKSMYPREIRMIQSYVSEACDRMDYQNSPMYDEWPEQRMVD
                     QMCNSVCEAVSTSGDMGILREAWEMQEKKTIERMSVEEEQEEEVVDQEKKRVEKGTDR
                     EKEKVETEELNDEENKKNDGKKKTMDERGEENIKEGAEENKNPMESDMEENIYQVTAE
                     ADLREHNRIIESQEMRPPLRNWWNGWGINGIRPDVQGMAWGMGSMYAPVVPVETENGP
                     VLLGQELRRPP"
     gene            complement(805..1317)
                     /locus_tag="ACZPCG_12710"
     CDS             complement(805..1317)
                     /locus_tag="ACZPCG_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002573076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HIR1285943.1"
                     /translation="MENSMLFELPFDPAYIIVILSGLVVILLILVIVAMAKLKKLSRK
                     YDYFMRGKDAETLEDVILDQLEEIRILKAEDRAAKDSIRTLNRNFRSSFQKFGMVRYN
                     AFPGMGGTLSFVIAVLDYTNSGFILNCIHSKEGCYLYMKEVDAGKTEKVLGNEEQEAL
                     EQALGYVKKN"
     gene            complement(1373..2269)
                     /locus_tag="ACZPCG_12715"
     CDS             complement(1373..2269)
                     /locus_tag="ACZPCG_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006778085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIR1285944.1"
                     /translation="MAAKRGLGKGLGAIFGEEVIKEQKEKIAMEDGKGKELMVKLSLI
                     QPNKEQPRKTFDQEKLKELSASIKRYGILQPLLVKKNGNFYEIIAGERRWRAAQMAGL
                     KEAPVVLKEYSRQEAMEISLIENVQRADLNPIEEAKAYQQLIREFNLTQEEIAERVAK
                     NRATITNTMRLLKLDEQIQEMLIQGIISSGHARALLSLDGGGLQLKAAKEIIEKSLSV
                     RETERLVKRMAKEAAGEEKKEKKADDALMLIYQNLEDRMKTIMGTKVSIHNKDKNKGR
                     IEIEYYSEAELERIVELIESIH"
     gene            complement(2269..3036)
                     /locus_tag="ACZPCG_12720"
     CDS             complement(2269..3036)
                     /locus_tag="ACZPCG_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR1285945.1"
                     /translation="MGRIITIANQKGGVGKTTTAINLAACLAEAGQHVMLVDFDPQGN
                     ASSGLGLEEKDFEQTVYDMMAGEASIKECITKEVQENLDVLPSDMNLAGAEIEFQELD
                     EKEILLKKSLDMVRDEYDFILIDCPPSLNILTINALTAADTVLVPIQCEYYALEGLNQ
                     MLKTVDLVKRKLNPSLELEGVVFTMYDARTNLSLQVVESVKSNLNQTIYKTIIPRNVR
                     LAEAPSHGMSINHYSSRSTGAESYRMLAAEVISRGEV"
BASE COUNT          764 a          890 c          470 g         1095 t
ORIGIN      
        1 ggggcggacg tctgagttcc tgtcccagta gcaccgggcc attttcagtt tccacaggaa
       61 caaccggggc atacatggat cccatccccc aagccattcc ctgcacgtcc ggcctaatcc
      121 cattgatccc ccagccgttc caccaattcc tcaatggagg tctcatttcc tggctttcaa
      181 ttattctgtt atgttctctt aaatccgcct ctgctgttac ctgatagata ttttcttcca
      241 tgtcactttc catgggattc ttattttctt ctgccccttc ttttatattc tcctctcccc
      301 tctcatccat agtctttttt ttaccatcat ttttcttgtt ttcttcatca tttaactcct
      361 ctgtttcaac cttttctttt tcccgatctg tacctttttc cacccttttt ttttcctgat
      421 ccactacttc ctcttcctgt tcttcttcca cgctcattct ttcaatcgtc tttttctcct
      481 gcatctccca cgcctctctt aaaatcccca tgtctccgga tgtactcaca gcctcacaaa
      541 cggaattgca catttgatct accattctct gttctggcca ctcatcatac atagggctgt
      601 tttgataatc cattctgtca catgcttccg atacatagct ctgtatcatc cggatttctc
      661 tgggatacat acttttaaaa ttttccattc atctgtcctt acccatatct ttcactttta
      721 tcatatgcag aaaccataaa aaatgtttca cgtgaaacat aaaaaaggat atctgagttt
      781 atattcccag atatccctgc atcatcaatt tttctttaca tatcccaatg cctgctccag
      841 agcttcctgc tcctcatttc ccagcacctt ttcagtcttg cctgcatcta cttccttcat
      901 atataaatag cagccttcct tactgtgaat acaattcaaa ataaagccac tgttcgtata
      961 atccaaaact gcaattacaa agctcagtgt tcctcccatt ccaggaaatg cattatatcg
     1021 aaccattcca aatttttgaa aggaagaacg gaagtttcga ttcaacgttc gaatcgagtc
     1081 cttggctgcc cgatcctctg ccttcaaaat acgaatttcc tccagctgat ccagtatcac
     1141 atcctccagt gtttccgcat cctttccccg cataaaataa tcatatttcc gactcaattt
     1201 tttcagcttt gccatagcaa caattacaag aatcaataga atcactacca agcctgacaa
     1261 tattacaata atatatgccg gatcaaatgg aagttcaaat agcatactgt tttccattct
     1321 tatctaatct ctccttactc ttatccgaat gttagataca gaatctttat ttctagtgga
     1381 tggactcaat caattccaca attcgttcca actctgcctc tgaatagtat tcgatttcta
     1441 tacggccttt gttcttatcc ttattatgta tgctgacctt tgttcccata atggttttca
     1501 ttctgtcttc cagattttga taaatcagca tcagggcatc atccgctttc ttttccttct
     1561 tttcttctcc agcagcttcc ttggccatcc gttttaccaa ccgttctgtt tcccggacac
     1621 ttaagctttt ttctataatt tcctttgccg cctttaactg cagcccccca ccgtccagag
     1681 aaagaagcgc tctggcatga ccgctgctaa taataccctg gatgagcatt tcctgaatct
     1741 gctcatccag ctttaaaaga cgcatggtat tcgtaatggt cgctctgttt tttgctacac
     1801 gctctgcaat ttcctcctgc gtgagattaa actcacgaat cagctgctga tacgccttcg
     1861 cctcttcaat aggattcaga tctgcccgct gaacattttc aatcagagaa atctccattg
     1921 cttcctgtcg gctgtactcc tttaaaacca ccggcgcttc cttgagtccc gccatctggg
     1981 cagctctcca tcttctttct ccggcaataa tctcgtaaaa gttcccattt ttctttacaa
     2041 gaagcggctg aagaatcccg taccgtttga ttgaggcaga taactcctta agcttttcct
     2101 gatcaaatgt ctttcttggc tgctccttat taggctgaat cagagaaagc tttaccatca
     2161 gttcctttcc ttttccatct tccatcgcaa tcttttcttt ctgttcctta atcacttctt
     2221 ccccaaagat tgcgcccaaa ccttttccca aacctctttt agccgccatt atacctctcc
     2281 tctgctaatc acttctgctg ccagcatacg atagctctcc gcacccgtag aacgggacga
     2341 atagtgattg atggacattc catgacttgg agcttctgca agccgcacat ttcttggaat
     2401 gattgtttta tagattgtct gattcagatt actcttcaca ctctccacta cctgaagaga
     2461 cagattggtt ctggcatcat acatagtaaa tactacgcct tccagttcca atgacgggtt
     2521 cagctttctc ttcaccaaat ctaccgtctt taacatctga ttcaatccct ccaatgcgta
     2581 atactcacac tggattggca ccagcacggt atcagctgct gtcaaagcat taatcgtaag
     2641 tatatttaat gaaggcggac aatcaattaa aatgaaatca tactcatccc gtaccatgtc
     2701 taacgatttc ttaagcagaa tctctttctc gtccaattcc tgaaattcaa tctccgcacc
     2761 cgccagattc atatccgaag gaagtacatc cagattttcc tgaacctctt ttgtgatgca
     2821 ctcctttata gaagcctctc cagccatcat atcataaacg gtctgttcaa aatccttttc
     2881 ttccaggccc aggccgctgc tggcatttcc ctgtgggtca aaatccacca gcattacatg
     2941 ctgtcctgct tctgcaaggc aggctgccag attgatcgcc gtggttgttt ttcctactcc
     3001 gcccttctga tttgctattg taataatccg tcccatatcc gtttcctcac gttcgttttc
     3061 gatgtaccag acgattataa cacaactttt ctgcttttcc tatgaaaaac tctgcatttg
     3121 tatttatttt tctattttat acaaaatatt acatgagtaa tgtttcacgt gaaacattac
     3181 catctgtaga caagggagca ctgtgttctg gtgtttagg
//
LOCUS       DBMDUH010000218         3195 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_10472_length_3195_cov_2.928571, whole genome shotgun sequence.
ACCESSION   DBMDUH010000218 DBMDUH010000000
VERSION     DBMDUH010000218.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10472_length_3195_cov_2.928571"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            98..1336
                     /locus_tag="ACZPCG_12725"
     CDS             98..1336
                     /locus_tag="ACZPCG_12725"
                     /inference="COORDINATES: protein motif:HMM:NF019604.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1285946.1"
                     /translation="MTRGYSDETQQGYTMDTVMEEAQRCLLCLDAPCSAACPAGTDPA
                     RFIRSVRFRNLKGAAETIRENNAIGGVCARVCPTERYCQQACSRCGIDRPIDIGGIQR
                     FVTDFEETVGMEILKTGAANGKKIGIIGSGPSGLQAAAALLGLGYEVHIYEKSEKAGG
                     YLRYGIPEYRLPERVVDREIEKIEKMGAEFHCGTDIGTDCTMDELKETYDAVLVAAGA
                     SVGKWLPMFEGNDRTETAVEFLARVKAKGGKEEVPDHVVVIGGGDVAMDAATTLKRLG
                     AKRVTDVAYEELCEFLASEKELEGARQAGVTIIDGYIPEQVDGNTVTFKHRHMDARLC
                     LGADKIILAVGQKPDVKSLGLEMEGNEAKADGYRTADPEVFVTGDLAEGDKTVVWAVR
                     KGKEAAQAIHESLQERGSRL"
     gene            1333..2580
                     /gene="preA"
                     /locus_tag="ACZPCG_12730"
     CDS             1333..2580
                     /gene="preA"
                     /locus_tag="ACZPCG_12730"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HIR1285947.1"
                     /translation="MISKDLSIDFLGVHFENPFCLSSSPVGNCYDMCKKAYDTGWGGV
                     VFKTIGPKHFLVDEVSPRFAALTKEGTPFVGFKNMEQIAEHPLEENLADVKRLKEEYP
                     DKVLIASIMGTNEEDWEELARLVTEAGADMIEMNLSCPQMTSHAMGSDVGTNPELCRT
                     YCAAVKRGSSLPILAKMTPNITDMVGVAKACLEGGADGISLINTVKSITDVDLERKIG
                     MPVVNGKSSISGYSGKAVKPIALRFIQQLRTAEGLENLPISGIGGIETWEDAAEFILL
                     GSSTLQVTTAIMQYGYRIVEDMKNGLMHYMEEQGVDHLSELVGLANQNIIPAESLDRD
                     YIVYPKIDEDTCIGCGRCYISCYDGAHQAMEWNAETRIPKCNTDKCVGCHLCALICPV
                     HAISKGEIVIKPGRKGEPQDKKV"
     gene            2585..3055
                     /locus_tag="ACZPCG_12735"
     CDS             2585..3055
                     /locus_tag="ACZPCG_12735"
                     /inference="COORDINATES: protein motif:HMM:NF018232.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HIR1285948.1"
                     /translation="MRKDPYHCSERCRCAFATAFLCMIWMLAASFFGLPGWAGFTGCT
                     AYFAAPGKGVKSLPSTFACVGSGIAYALVSMAVSQGGNGPAAGLVMTFLTTFLMCAGS
                     GNRILGFVPGAFMGSFSMFAAGGDLKAAAAILIGVLLGLFCDMFGKFLCSMKKE"
BASE COUNT          869 a          604 c          941 g          781 t
ORIGIN      
        1 gagtaaacag aggacgctct aagggaacag tggcagaata gctgcaggat tttgccataa
       61 gtgcttcaac agctgttgtt gaggaaagga gataggaatg accagaggat attcagatga
      121 gacacaacag gggtatacaa tggatacggt catggaggag gcacagcggt gtctgctttg
      181 ccttgatgcg ccatgttctg cggcatgtcc ggcgggaacg gatccggcca gatttatcag
      241 aagcgtacgg tttcgcaatc tgaaaggagc cgctgagacg atccgcgaaa acaatgcaat
      301 aggcggcgta tgtgcgagag tctgtccgac ggaacgttac tgccagcagg cgtgctcccg
      361 ctgcggaatt gaccgcccga ttgatattgg aggcatccag cggtttgtaa cggattttga
      421 agagacggtt ggaatggaaa tcttaaaaac gggtgctgcg aacggaaaaa agattggcat
      481 tattggaagc gggccttccg ggcttcaggc ggcagctgcg cttttggggc ttggatatga
      541 agttcatatt tatgagaaat ctgaaaaagc cggcggatat cttcgctatg ggattcctga
      601 ataccgtctt ccggagcgtg tggttgaccg tgagatcgag aagattgaaa aaatgggagc
      661 agagtttcac tgcggtacgg atataggaac ggactgcacg atggatgagc tgaaagaaac
      721 gtacgatgcg gttctggttg ccgcaggagc cagtgtcgga aaatggcttc cgatgtttga
      781 gggcaatgac cggaccgaga ctgctgtgga atttctggca agagtaaagg caaagggcgg
      841 aaaggaagaa gtaccggatc atgtagtcgt tattggcggc ggcgatgtgg ctatggatgc
      901 ggcaacaacg ctgaaacgtc ttggggcaaa gagagtcacg gatgtagcat atgaggagct
      961 ttgtgaattt ttagcttctg aaaaagaact ggaaggcgca aggcaggcag gagttaccat
     1021 tattgacgga tatattccgg aacaggtgga tggaaataca gtaactttta agcaccgcca
     1081 tatggatgcc agactttgcc ttggtgcaga taagattatt ttagctgtag ggcagaaacc
     1141 ggacgtcaaa tcccttggat tggaaatgga agggaatgaa gcgaaagcag acggatatcg
     1201 gaccgccgat cccgaggtat ttgttaccgg agatctggca gagggagata agacggttgt
     1261 ttgggccgta aggaaaggaa aagaagcagc acaggcaatc catgagagcc ttcaggaaag
     1321 ggggagccgt ttatgatcag taaggattta tctattgatt ttttgggagt tcactttgaa
     1381 aatccatttt gtttatcctc ttctccggta ggaaattgtt atgatatgtg caaaaaagca
     1441 tatgataccg gatggggcgg cgtagtattt aaaaccattg ggccaaaaca cttcctggtt
     1501 gatgaggtat cgccgcggtt tgctgcattg acaaaggaag gaacaccgtt tgtaggtttt
     1561 aagaacatgg agcagattgc agaacatccg ctggaagaaa atctggcaga tgtaaagcgg
     1621 ctgaaggaag aataccccga taaagttctg attgcttcca ttatgggaac aaatgaagag
     1681 gactgggaag agctggcacg gcttgttaca gaagccgggg cagatatgat tgagatgaac
     1741 ctttcctgcc ctcagatgac atcacatgcg atgggctccg atgtgggaac aaatccggaa
     1801 ctgtgccgta catattgcgc agcagtaaaa agaggttcct cccttcctat tctggcaaag
     1861 atgacaccga atattacgga tatggtaggc gtcgccaaag cgtgcctgga aggaggggca
     1921 gacggtattt ctctgatcaa tacagtcaaa tcgattacag atgttgatct ggagcgaaag
     1981 attggtatgc cggttgtcaa cggaaaatca tccatttccg gttattcagg aaaggcggta
     2041 aaaccgatcg ccctgcgttt tatccagcag ctgagaacgg cagaaggatt ggagaacctt
     2101 ccgatttccg gaattggagg aattgaaacc tgggaggatg cggcggaatt tattctttta
     2161 ggatcgtcaa cccttcaggt aacaacagca attatgcagt atggatacag aattgttgag
     2221 gatatgaaga acggtctgat gcattatatg gaggagcagg gagtcgatca tttaagcgaa
     2281 ctggttggat tggcaaatca gaatattatc ccggcagaat ctctggaccg cgattatatt
     2341 gtttatccaa agatagatga agacacctgt atcggatgcg gacgctgtta tatttcctgc
     2401 tatgacggag cgcatcaggc gatggaatgg aatgcagaga cgcgtatacc gaaatgtaat
     2461 acggataagt gtgtaggatg ccatctctgc gcgctgatct gtccggttca cgcaatttcc
     2521 aagggtgaga ttgtaattaa gccgggaagg aaaggagagc ctcaggacaa gaaagtgtga
     2581 tgttatgagg aaagatccgt atcactgcag cgaacgctgt cgctgtgcgt ttgcaacagc
     2641 ctttctttgt atgatctgga tgcttgcagc ctcctttttc ggactgcctg gctgggctgg
     2701 ctttaccggc tgcaccgctt attttgcggc gccgggaaag ggggtaaaga gccttccgtc
     2761 aacctttgca tgtgtgggga gcgggattgc atatgcgctt gtttccatgg ccgtgagtca
     2821 gggggggaat ggcccggcag cagggcttgt gatgaccttt ttaactacct ttttgatgtg
     2881 tgcgggcagc ggaaaccgta ttttgggatt cgttccgggt gcttttatgg gatccttttc
     2941 catgtttgct gccggaggtg acttaaaggc agcagcagct atcctgatcg gcgttcttct
     3001 tggacttttc tgtgatatgt ttggaaaatt cctgtgcagc atgaaaaaag aataaaggat
     3061 tcccgatgca aagcggtttt ctgacggaat gatccgtaaa aagggacaac accggctgta
     3121 tataacggtg ttgtcccttt taaaaacgat tagtagaata aattgaccaa tccggtcgaa
     3181 aataccggaa cgtat
//
LOCUS       DBMDUH010000219         3154 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_10646_length_3154_cov_5.572879, whole genome shotgun sequence.
ACCESSION   DBMDUH010000219 DBMDUH010000000
VERSION     DBMDUH010000219.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10646_length_3154_cov_5.572879"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1098
                     /locus_tag="ACZPCG_12740"
     CDS             <1..1098
                     /locus_tag="ACZPCG_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002603038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase family protein P"
                     /protein_id="HIR1285949.1"
                     /translation="AVLNDEICGYLESMGVVILPYNDIYKEAGALQGEKVLFEREKGN
                     YALYQAMAAQNEIIDRMNPTSEAKAKKNPVEVENEKKAHIKDGIAVTRFMYWLKHQIG
                     KETITEMSASEKLEGFRKEQEGYIEPSFNTISAYGPNAAMCHYSATEESNAVLDAKSF
                     YLVDSGGQYYEGTTDITRTIALGELTAEEKEHFTLVLISMLRLQAVRFLYGCRGLSLD
                     YVAREPFWSRGLNFDHGTGHGVGYLSSVHERPNGIRFKMVPERCDNGILEEGMITSDE
                     PGLYIEGKHGIRTENLILCKKDEKNEYGQFMKFEFLTFVPIDVDAVDKNLMTERDVEL
                     FNAYHKEVYEKISPYLEGDEKEWLKKVTAAI"
     gene            complement(1233..1823)
                     /locus_tag="ACZPCG_12745"
     CDS             complement(1233..1823)
                     /locus_tag="ACZPCG_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HIR1285950.1"
                     /translation="MTNQLQPPLSYHIDPTIYARRPADSSGRLPKELRTYDLLDSLHI
                     SYERVDHDALPTIEACRQVDEILGTPMCKNLFLRNAQKTAFYLLLLPGEKKFKTAALS
                     KQIGSARLSFGEPEFMEQLMDLTPGSVTVLGLMNDAENKIRLLIDRDVIENHAFIGCH
                     PCINTSSLKLKTQDLITKILPAIHHDYTLVDLPDVK"
     gene            2013..>3154
                     /gene="rlmD"
                     /locus_tag="ACZPCG_12750"
     CDS             2013..>3154
                     /gene="rlmD"
                     /locus_tag="ACZPCG_12750"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HIR1285951.1"
                     /translation="MEKREANERIKRTDAGKNKKDVRPDGKKKDAGRAGVPERKQKSL
                     CPVSKKCGGCQLLDMPYSQQLTLKKKQLEETLKGICKVQTVIGMEQPFHYRNKVHAVF
                     DRDRRGNIISGIYQENTHIVVPVEKCLIEDEKADEIIGTIRGMLKSFKIRIYDEDTGY
                     GLLRHVLIRKGFQSGEIMVVLVTASPVFPSKNNFIKALRENHPEITTIVQNINGRGTS
                     MVLGDREQVLYGKGYITDQLCGCTFRISSKSFYQVNPVQTEKLYNLAIELAGLTGKET
                     VLDAYCGIGTIGIIASRKAGQVIGVELNQDAVRDAVNNAKLNQISNIRFYCNDAGRFM
                     VNMAERGDRADVVIMDPPRSGSTEEFMDSIKKMGADKLVYISCNPET"
BASE COUNT          935 a          564 c          884 g          771 t
ORIGIN      
        1 gctgttctta atgatgagat ttgtggatac ttggagtcta tgggtgttgt gattcttcca
       61 tataatgata tctataagga agcaggagcg cttcagggag aaaaggtgct gtttgagcgg
      121 gagaagggaa attatgccct gtatcaggct atggcagcgc agaatgagat cattgaccgg
      181 atgaacccta cgtcagaggc aaaggccaaa aagaatccgg tggaagtgga gaatgagaag
      241 aaggcccata ttaaggacgg cattgcagta accaggttta tgtactggtt aaagcatcag
      301 attggaaaag aaaccattac agagatgagt gcgtctgaaa agctggaggg cttcagaaag
      361 gagcaggagg gatatatcga gcccagcttc aataccattt ctgcctacgg ccccaatgcg
      421 gccatgtgcc attattccgc tacagaggaa agcaatgcag ttttagatgc aaaaagcttt
      481 tatctggtgg attccggcgg ccagtactat gagggaacca cagacatcac aagaaccatt
      541 gcactgggag aactgacagc agaagaaaag gagcatttta ccctggttct catcagtatg
      601 ctccgccttc aggcagtacg gtttttatac ggctgcagag gcttaagcct ggattatgtg
      661 gcaagagagc cattctggag caggggactg aattttgacc acggaaccgg acatggtgtg
      721 ggatatcttt caagtgtcca tgagcgcccc aacggaatcc gtttcaaaat ggttccggag
      781 cgctgtgaca acggaatatt ggaggaggga atgattacct cggacgagcc gggactgtat
      841 atcgaaggaa agcatggaat ccgtacggaa aatctgattc tctgcaaaaa ggatgagaag
      901 aatgaatacg gtcagtttat gaaatttgaa ttcctgacct ttgtccctat tgatgtggac
      961 gcagtggaca agaacctgat gacagagcgg gatgtggagc tgtttaatgc atatcataaa
     1021 gaggtttatg agaagatcag tccgtatctg gaaggggacg agaaggagtg gcttaagaag
     1081 gtaacggctg cgatttaata caggaggaat agagatttgg aagcatcttc catattttcg
     1141 gagcattttg taatgtaact gtgtaaatag aaagagacgt cgtttcacgc tgtttaggcg
     1201 aggaatgcgt ctctttttta tggataacag acttatttta catccggaag gtctaccaga
     1261 gtatagtcgt gatggatggc aggcagaatt tttgtaatca gatcttgtgt ttttagttta
     1321 aggctggagg tgttaataca ggggtgacag ccgataaaag catgattttc tattacgtcc
     1381 cggtcaatga gaaggcggat cttattttcg gcatcattca taagccccag aaccgtaacc
     1441 gaaccgggag tcagatccat cagctgttcc ataaattccg gttctccaaa ggacaggcgg
     1501 gcgcttccaa tctgcttgga cagggcggct gtcttaaact ttttctcacc gggaaggaga
     1561 agaagataaa aggcagtttt ctgggcattg cgcagaaaca ggtttttgca catgggggtt
     1621 cccagaattt catccacctg tctgcaggct tcaatggtgg gaagggcgtc gtgatctaca
     1681 cgctcgtagg aaatatgcag ggaatccaga aggtcatagg ttctcagttc cttgggaagg
     1741 cgtccggagg aatcggcggg gcgtctggca tagatggtgg gatcaatatg gtaagataac
     1801 ggtggttgta attggtttgt cataggaata cctcgtattt ttgcagtgtc tgtgcggcag
     1861 aattaaatgc agtgaaggaa taaccgtgcg gttggggcag ctgtcggata tacagcggtt
     1921 ttgttacaca ttataaggaa tctgtgataa aatgtcgata gcagataaga aagaatataa
     1981 tagatttatg aaaaaggaga gtttgtaaat atatggaaaa gagagaggcg aatgaacgaa
     2041 ttaagagaac agatgcagga aagaataaaa aagatgtaag gccagatgga aaaaagaagg
     2101 atgcaggaag agccggcgta ccggaaagaa aacagaaaag tctctgtcct gtaagcaaaa
     2161 aatgtggtgg ctgtcagctg cttgatatgc cctacagcca gcagctgaca ttaaagaaaa
     2221 agcagctgga agagacatta aagggaatct gcaaggttca gactgtgatt ggaatggaac
     2281 agccatttca ttacagaaat aaggttcatg cggtgtttga ccgcgaccgc agaggaaata
     2341 ttatttccgg gatttatcag gaaaataccc atatcgtagt gcctgtggaa aaatgcctga
     2401 tcgaggatga aaaggcggat gaaattatcg ggactatccg gggaatgctt aaatccttta
     2461 agatccggat ctatgatgag gatacgggat acggccttct gcgccatgtg ctgatccgga
     2521 aaggatttca gtcaggggag atcatggtgg tactggttac tgcatcaccg gtatttcctt
     2581 caaagaacaa ttttataaag gcacttcggg aaaatcatcc ggagatcaca accattgtac
     2641 agaatatcaa tggccgggga accagtatgg tattgggaga ccgggagcag gttctttatg
     2701 gaaagggata tatcacggac cagctctgtg gctgtacctt ccgtatttct tcaaaatcct
     2761 tttatcaggt gaatccggta cagacagaga agctctataa tctggccatt gagttggctg
     2821 ggcttacagg aaaggaaaca gtgctggatg cctactgcgg gatcggaacc attggcatca
     2881 tagccagccg caaagccggt caggtgatcg gagtggagtt aaatcaggat gcagtccggg
     2941 atgctgtaaa taacgcaaag ctgaatcaga tttccaatat ccgtttttac tgcaatgatg
     3001 ccggccggtt tatggtaaat atggcggagc ggggagaccg ggccgatgta gtcatcatgg
     3061 atccgcccag aagcggaagc acagaggaat ttatggactc cattaagaaa atgggggcgg
     3121 ataagctggt gtatatttca tgtaatcctg agac
//
LOCUS       DBMDUH010000220         3092 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_10932_length_3092_cov_7.212304, whole genome shotgun sequence.
ACCESSION   DBMDUH010000220 DBMDUH010000000
VERSION     DBMDUH010000220.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10932_length_3092_cov_7.212304"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="ACZPCG_12755"
     CDS             complement(<1..561)
                     /locus_tag="ACZPCG_12755"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015574089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HIR1285952.1"
                     /translation="MKLLYSCIIAISMYSRIPVPQIQWTKERMKHALCFFPLVGLIQG
                     LFLALWLWLAEEVFRLSPAMTGLWAAALPVLITGGIHMDGFLDTMDGVCSYGDREKKL
                     EILKDPHLGAFAVIYTFAYMLFYAGASCEFAARAEGSARLLPVLFMTMERALSGLSVL
                     SFPSAKKDGLAASFAQAAEKKTDRMGM"
     gene            complement(558..1097)
                     /locus_tag="ACZPCG_12760"
     CDS             complement(558..1097)
                     /locus_tag="ACZPCG_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HIR1285953.1"
                     /translation="MILITGGSGSGKSEYGEYRVMEEQMPLRFYVACMEVYGEEGRRK
                     VERHRALRAGKGFVTLERPRGLSGLSLKGEGKKAVILECVSNLAANAMFEKEGIQPDT
                     EELAEGLFDDILYLDSQADFLAVITNEVGADGEDYTPETMEYIRLMGRLNCLLAERAS
                     EVVEVVYGIPVVLKGEKGK"
     gene            complement(1094..2167)
                     /gene="cobT"
                     /locus_tag="ACZPCG_12765"
     CDS             complement(1094..2167)
                     /gene="cobT"
                     /locus_tag="ACZPCG_12765"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HIR1285954.1"
                     /translation="MTLKKALQQIRPVSSETKKAARLRWDQVAKPLNSLGILEEDLVR
                     IAGAQNSEAVCLDQKWIAVMCADNGIVEEGVTQTGQEVTAVVAANMARGASCVCLMAA
                     KAGAKVLPVDVGIASDMEGTGILNRKIRYGTRNFLKEPAMTREETVKAMETGMEAAGI
                     LKEKGCRLAGCGEMGIGNTTTSSAVLSVLLGKEPEEVTGRGAGLSDEALNRKIRVIRE
                     GIMLCHPDPEDAIDVLSKVGGLDLAALAGFYIGCACYRIPVVLDGLITAAAALAAVKL
                     SPTVRDYLLASHVSAEPAGAMALEALGLKAAIHGEMCLGEGTGAAALFPLLDMAAAVY
                     GQMESFEEIHVEQYRRFGKEEEE"
     gene            complement(2188..>3092)
                     /locus_tag="ACZPCG_12770"
     CDS             complement(2188..>3092)
                     /locus_tag="ACZPCG_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cobyrinic acid a,c-diamide synthase"
                     /protein_id="HIR1285955.1"
                     /translation="VERETGIRAAGFVPELDFLKVGSRHLGLVLPEEIAGLREQMNRL
                     GKCLEETIDWEFLEELGAEKEGRDALEEENTEASCTAAFSFRLGIAMDEAFCFYYQDN
                     LRLLERLGGELVYFSPIHDRSLPEQLDGLILGGGYPELYCEALSLNESMRESVKKAAE
                     GGLPVLGECGGYLYLLEELEAEDGRIWPMTGVLKGKGYKKGKNSRFGYIGVEAEKDSL
                     YLKPGEQIRGHEFHYWDCEVLEEEWVMRAKKPVGNRSWPCIRIKNQVMAGFPHLFYPS
                     CPAFAVRFAKACVRYKRKRDEAEE"
BASE COUNT          694 a          999 c          639 g          760 t
ORIGIN      
        1 cattcccata cggtctgttt tcttctctgc agcctgtgca aaagaggctg ccaggccgtc
       61 ttttttggca gaaggaaagg aaagcaccga caggccgctg agggcccgct ccatggtcat
      121 aaaaagcaca gggagcagcc gtgcgcttcc ctctgccctt gctgcaaatt cgcaggaggc
      181 tccggcataa aagagcatat atgcaaacgt atagataacc gcaaaagccc ccagatgggg
      241 gtcctttaaa atttccagct ttttttcccg atccccatag gagcacactc cgtccatggt
      301 atccagaaag ccgtccatat gaatccctcc cgtgataagc acaggaagag ccgccgccca
      361 caggccggtc atagcaggag acaaacgaaa gacctcctct gccagccaca gccacagcgc
      421 caggaacagg ccctggatca gacccaccag aggaaaaaag cataatgcat gcttcatccg
      481 ctccttcgtc cattggatct gaggcacagg aatcctggag tacatggaaa ttgctatgat
      541 acaggaatac agaagcttca ttttcccttc tctcctttca ggacaaccgg aatcccataa
      601 accacctcaa ctacttcact ggccctttca gccagcaggc agttaagccg ccccataagg
      661 cggatatact ccatggtttc cggcgtatag tcctccccgt ctgctcccac ctcatttgtg
      721 ataaccgcca gaaaatcagc ctgggaatcc agataaagaa tatcgtcaaa gagcccttct
      781 gccagttcct ctgtatccgg ctgtatgccc tccttttcaa acatggcgtt ggcagccaga
      841 ttggacacgc actctaaaat gacagccttt tttccttctc cttttaagct tagtccggac
      901 aggcctctgg gcctttcaag ggtcacaaag ccttttccgg ctctgagagc cctgtggcgt
      961 tctaccttcc tgcgcccctc ttccccgtaa acctccatgc aggccacata gaagcgaagg
     1021 ggcatctgct cctccatcac cctatattcc ccatattcgg attttccgct gccgctgccc
     1081 cctgtgatca ggatcattct tcctcttcct ttccgaaacg gcggtactgc tccacatgga
     1141 tttcctcaaa tgactccatc tgtccgtaca cagccgcagc catgtcaaga aggggaaaca
     1201 gcgccgcggc tcccgttccc tctcccagac acatttctcc gtggatcgca gcctttaagc
     1261 ccagcgcctc taaggccata gctcccgcag gctctgcgga tacgtgagaa gccagcagat
     1321 aatcccttac cgtcggagaa agcttcacag ccgccagagc agccgcagcc gtaatcaatc
     1381 cgtccagcac caccgggatc cgataacagg cacagccgat ataaaaaccg gccagagcag
     1441 ccagatcaag gccgccaacc ttggaaagca cgtcgatagc gtcctccgga tccggatggc
     1501 aaagcatgat tccctcacga atgacacgga ttttccggtt cagcgcctca tccgaaagtc
     1561 ctgccccccg gccagtcacc tcttccggct cctttcccaa aaggacagaa aggacagcgc
     1621 tggacgtagt tgtatttcca attcccattt caccgcagcc tgccagccgg cagccctttt
     1681 cttttagaat ccctgccgct tccatccctg tttccatggc ttttaccgtt tcctcccgcg
     1741 tcatggcagg ctctttcagg aaattccggg ttccatagcg tattttccgg tttaaaatcc
     1801 ccgtcccttc catatctgag gcaattccca catccacagg aagtaccttt gcccctgctt
     1861 tcgcagccat caaacagacg caggaggccc ctcttgccat atttgccgct actacggctg
     1921 ttacctcctg tccggtctgg gtaacgcctt cctccacaat gccgttgtct gcgcacatga
     1981 cggcaatcca cttctgatcc agacagaccg cctcgctgtt ctgagccccg gcaatgcgta
     2041 ccagatcctc ttccaggatc cccaagctgt ttaagggctt tgccacctgg tcccagcgaa
     2101 gccgggccgc cttctttgtc tcactgctta ccggacgtat ctgctgcagc gcttttttta
     2161 gcgtcatttt tccttctgta ttttgtatca ttcttctgcc tcatctcttt tccgtttgta
     2221 tcttacacag gctttggcaa aacggacggc aaaagccggg caggaggggt aaaataaatg
     2281 aggaaaaccg gccatcacct gattctttat gcgtatacag ggccagcttc tgtttcctac
     2341 cggctttttt gctctcatta cccattcttc ttccaaaacc tcgcaatccc agtaatgaaa
     2401 ttcatgaccc ctgatctgtt ctcccggctt taaatacagg ctgtcttttt ccgcttccac
     2461 tccaatgtag ccaaaacggc tatttttccc ctttttatat ccttttccct tcaaaacgcc
     2521 tgtcatgggc cagatccgtc catcttccgc ctccagctcc tccaaaagat agagatatcc
     2581 tccgcactct cccagaacag gaaggccccc ttctgccgcc tttttcacag attcccgcat
     2641 ggattcattc agcgaaagcg cctcgcagta aagctccgga tacccgcctc ctaagatcag
     2701 gccgtccagc tgttcgggaa ggctcctgtc atgaatgggg ctaaagtata ccagttctcc
     2761 tcccagacgc tctaaaagcc gcagattatc ctgataataa aagcagaatg cctcgtccat
     2821 ggcaattccc agacggaagc tgaaagccgc tgtacaggaa gcttctgtgt tttcttcctc
     2881 taaagcatcc cttccttctt tttctgctcc cagttcttcc agaaattccc agtctatcgt
     2941 ttcctccaga cattttccaa ggcggttcat ctgctctctt aatccggcaa tttcctctgg
     3001 cagcaccagt cccagatgac ggcttcccac cttgagaaaa tccagctccg gcacaaaacc
     3061 ggctgcccgg atgccggttt cccgttccac ca
//
LOCUS       DBMDUH010000221         3079 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_10986_length_3079_cov_5.678643, whole genome shotgun sequence.
ACCESSION   DBMDUH010000221 DBMDUH010000000
VERSION     DBMDUH010000221.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10986_length_3079_cov_5.678643"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            55..840
                     /locus_tag="ACZPCG_12775"
     CDS             55..840
                     /locus_tag="ACZPCG_12775"
                     /inference="COORDINATES: protein motif:HMM:NF019887.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HIR1285956.1"
                     /translation="MSDIDGTLIRRGEGFPKEVEQAAAELKKRGILFTFASGRLPYMI
                     TPFAESLGIEIPVCACNGTLLYRKDEILEKHPLCLLELRPLIEAALLREMTVLYSLNG
                     TEYCMRENEAVRRKRAERGSYHPIRPVREEEWENLWADKVNISDEQGEISSLLEWEQP
                     LEGSCEITHYGNQGLEIVRAGFGKAYGVRQICAGLGISMEQVLAIGDNENDNEMLTQA
                     GIGAAVGNAETATKACADLTARKESGAGATEIIRRICLEENEK"
     gene            837..1724
                     /locus_tag="ACZPCG_12780"
     CDS             837..1724
                     /locus_tag="ACZPCG_12780"
                     /inference="COORDINATES: protein motif:HMM:NF013429.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR1285957.1"
                     /translation="MKLSTSTNMVFERINMEPISQERGILLCVQAGYRVFDFCFHDLI
                     TFKSPFLDERWEAYTEKMCALKEEHGLSYEQGHANVYDFLNPKADHEFHQTIMERCVL
                     ASEKMGIPWLVVHPSTAFSADAVYAASRSGNTEYFKRLCEFAAKHGVGIAVENMWDLH
                     IAPKRYYADHAEELCELTDAVGAENIGICWDLEHASIMGQDQKKSLKIIGNRLKVTHV
                     SDQTGVDNIHVMPFQGKTDWKAAMEGLAEIGYEGNLNFEAQWFLNKVPEALLLPSLRY
                     SREVGAYLIGLYEEKRRLK"
     gene            complement(1733..2266)
                     /locus_tag="ACZPCG_12785"
     CDS             complement(1733..2266)
                     /locus_tag="ACZPCG_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005426074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HIR1285958.1"
                     /translation="MKNVLIISSSPRKNGNSQMLCEAFKKGVEESGNFAKLIRLAEKN
                     IGYCKGCDFCMKNDGVCVQKDDMSEILDAYKKADVLVLATPVYFHGVCAQMKTFIDRT
                     YPIWQNLGKKEIYYIVTAGLGRDIIECSLGDLDGFIFHLEKHEIKGRIYATDVTDVGA
                     VSKTPFINMAYQMGYCV"
     gene            complement(2378..>3079)
                     /locus_tag="ACZPCG_12790"
     CDS             complement(2378..>3079)
                     /locus_tag="ACZPCG_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1285959.1"
                     /translation="ENQPVYVANYTRIRPHMPLIRRILHIGIPAGIENSMFQFGKLAI
                     QSSVSTLGVTAMAAHAMASNFESFNGIAGIGVGIGLMTVTGHCLGAGRKDEAVYYVKK
                     FCVAAEIAVAGSCILLFLLARPIVTLSGMEPESMRLFLGLLTFITLVKPIIWVPSFIP
                     AYGLRAAGDVKYTMVVSCLTMWLCRVSLCIFLIRGLGVGPIAVWIGMFSDWIVRGIFF
                     TRRYMSRKWLEFHVL"
BASE COUNT          846 a          697 c          797 g          739 t
ORIGIN      
        1 ctaacgacta ggaaaaatat gacagaaaaa agaaaaaaag acatacgtct tgttgtgagc
       61 gacattgacg gaaccctgat ccgcagagga gaagggttcc ctaaagaggt ggagcaggcg
      121 gcggcagagt taaaaaagag ggggatcctc tttacctttg cctccggccg gctgccctat
      181 atgattacgc cttttgcaga gtcacttgga atcgaaatcc ccgtatgcgc ctgcaatggc
      241 acgcttcttt accggaagga tgagatactg gaaaaacatc ccctttgcct tttagagctt
      301 cgtcctctga tcgaggcggc gcttttaaga gaaatgaccg tactgtattc cttaaacgga
      361 acagaatact gtatgcggga aaatgaagcg gttcggcgca agcgggcgga gcgggggagc
      421 taccatccaa tccgcccggt gagggaagag gaatgggaaa acctttgggc ggataaggta
      481 aatatttccg atgaacaggg cgaaatttcc agccttctgg aatgggagca gcctcttgag
      541 ggaagctgtg agattaccca ttacggaaac cagggccttg agattgtacg ggccggcttt
      601 ggaaaggcct acggcgtaag gcagatctgc gccgggcttg ggatttccat ggagcaggtt
      661 ctggcaatcg gggataatga aaatgacaat gagatgctga cgcaagccgg tatcggagcc
      721 gcagtgggaa atgcagagac agctaccaaa gcctgtgcag acctgactgc cagaaaggaa
      781 agcggagccg gggcgacaga gatcatccgc cggatctgtc ttgaggagaa tgagaaatga
      841 aactgtctac ttcaaccaat atggtgtttg aacgcatcaa tatggagcct atttcccagg
      901 agcgcggaat ccttctctgt gtccaggcgg gatacagggt attcgatttc tgcttccatg
      961 acctgattac ctttaaaagc ccttttttag atgaacgctg ggaagcatat acggaaaaaa
     1021 tgtgcgcgct gaaggaggaa catggcctct cttacgaaca gggccatgcc aatgtatatg
     1081 attttttgaa tcccaaagcc gatcatgagt ttcaccagac catcatggag cgctgtgtcc
     1141 tggcctctga gaaaatgggg attccctggc ttgtggttca tccctctacg gccttttcgg
     1201 cagatgcagt ttatgcagcc tcccggtccg gcaatacaga gtattttaag cgtctctgcg
     1261 agtttgccgc aaagcacggc gtgggaatcg cggtggaaaa tatgtgggat ctgcatattg
     1321 cgcctaaacg ctattatgcg gatcatgcag aggagctgtg tgagctcaca gacgcggtgg
     1381 gcgcagaaaa tatcggcatc tgctgggact tggagcatgc cagcatcatg gggcaggatc
     1441 agaaaaaatc tctgaaaatc atcggaaacc gcctgaaggt cacccatgtg tccgaccaga
     1501 ccggcgtgga caatatccat gtaatgcctt tccagggaaa aaccgactgg aaggctgcca
     1561 tggaaggact ggcagagatt ggatatgaag ggaatctgaa ttttgaggcc cagtggtttt
     1621 taaataaagt tcctgaggcg cttttactcc cctccctgcg ctacagcaga gaggtgggag
     1681 cgtatctgat cgggctttat gaggaaaaaa gaaggcttaa ataatcggag gcttaaacgc
     1741 agtatcccat ttgataagcc atattgataa agggcgtttt gctgacagca ccgacatccg
     1801 taacgtctgt cgcatagata cgccctttta tttcgtgctt ttccagatga aaaataaaac
     1861 cgtccagatc tcccagagag cactcgatga tatcgcggcc taaaccggct gtaacaatat
     1921 aatagatttc tttttttcct aaattctgcc atatgggata ggtacggtcg ataaaggttt
     1981 tcatctgggc gcaaacgccg tggaagtata cgggcgttgc caataccaga acgtctgctt
     2041 ttttatatgc atctaggatt tcagacatat catctttttg gacacagact ccatcatttt
     2101 tcatgcaaaa atcacagcct ttgcagtatc caatgttctt ttctgccaga cggatcagtt
     2161 ttgcgaaatt tccgctttct tccacccctt ttttaaaagc ttcacacaac atctgagaat
     2221 tcccattttt tctggggctg cttgaaataa taagtacatt tttcatttgg tacctcctga
     2281 taaagcgttt ttgtttgata ttaccataaa atacggagat tttctatctt aaattataaa
     2341 aaatgaaaaa gagagagggg aaacggcagg ggttatgtta aagcacatga aactccagcc
     2401 attttctgct catataccgt cttgtaaaga agattcccct cacgatccag tcggagaaca
     2461 taccgatcca cacggcgata gggcccacgc caaggccgcg gatgagaaaa atacataagg
     2521 acacccggca cagccacatg gtaagacagg aaacgaccat ggtatatttt acatctcctg
     2581 cggccctcag gccataagcg ggtatgaagg acggtaccca gatgatcggt tttacaaggg
     2641 tgatgaaggt caaaagtccc agaaacagac gcatgctttc gggctccatg cctgaaaggg
     2701 tgacaatggg gcgggctaaa aggaaaagaa ggatacagct tccggccacg gcgatttctg
     2761 ctgccacgca gaatttcttt acataataga ccgcttcgtc ctttcttccg gctcccagac
     2821 agtgtccggt aacggtcata aggccgattc ccacgccaat gcctgcgatc ccgttaaagc
     2881 tttcaaaatt ggaggccatt gcatgggctg ccatggcggt aacgccaagg gtagacacac
     2941 tggactggat agcaagcttt ccaaactgga acatactgtt ttcaatgcct gcgggaatac
     3001 cgatatggag aatccgccgg atcaaaggca tatgggggcg tatgcgggta taattggcta
     3061 catagaccgg ctgattttc
//
LOCUS       DBMDUH010000222         3001 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_11350_length_3001_cov_4.648621, whole genome shotgun sequence.
ACCESSION   DBMDUH010000222 DBMDUH010000000
VERSION     DBMDUH010000222.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11350_length_3001_cov_4.648621"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /gene="obgE"
                     /locus_tag="ACZPCG_12795"
     CDS             complement(<1..774)
                     /gene="obgE"
                     /locus_tag="ACZPCG_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HIR1285960.1"
                     /translation="MFADRAKVFIKSGKGGDGHVSFRRELYVPNGGPDGGDGGRGGDV
                     IFQVDTGKNTLIDFRHIHKYIAKDGQEGGKRRCHGADADDLIVKVPEGTVIKDFESGK
                     VIADMSGDNKREVILKGGKGGLGNMHFATSTMQVPKYAQPGQPGQELWVQLELKVIAD
                     VGLVGYPNVGKSTLLSVVSNAKPEIANYHFTTLNPHLGVVDLGDGAGFVMADIPGLIE
                     GASEGVGLGHSFLRHIERTKVLVHVVDAASVEGRDPLEDI"
     gene            complement(905..1195)
                     /gene="rpmA"
                     /locus_tag="ACZPCG_12800"
     CDS             complement(905..1195)
                     /gene="rpmA"
                     /locus_tag="ACZPCG_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HIR1285961.1"
                     /translation="MLKMNLQLFAHKKGVGSTKNGRDSESKRLGAKRADGQFVLAGNI
                     LYRQRGTHIHPGNNVGKGGDDTLFALVDGVVKFERKGRDRKQVSVYPRAINE"
     gene            complement(1199..1540)
                     /locus_tag="ACZPCG_12805"
     CDS             complement(1199..1540)
                     /locus_tag="ACZPCG_12805"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HIR1285962.1"
                     /translation="MIQATVLKDSRDSGNVLYRGIVIEGHAGYAEEGEDIICAAVSAL
                     AINFYNSVETFTEDPFEGAAGQEDVQFDFRFTSDISPESQLLMNSLILGLQNIEKDYG
                     RTYIIIRFKEV"
     gene            complement(1552..1860)
                     /gene="rplU"
                     /locus_tag="ACZPCG_12810"
     CDS             complement(1552..1860)
                     /gene="rplU"
                     /locus_tag="ACZPCG_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007718128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HIR1285963.1"
                     /translation="MYAIIATGGKQYKVAEGDVIKVERLGAGAGETVTFDQVLAVNNG
                     ELQIGCPTVAGATVTATVEKEGKAKKVIVYKYKRKTGYHKKNGHRQLYTQVKIEKINA
                     "
     gene            complement(1979..>3001)
                     /locus_tag="ACZPCG_12815"
     CDS             complement(1979..>3001)
                     /locus_tag="ACZPCG_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285964.1"
                     /translation="VKLLYQSEFGGGHMITSPEKSLDRLKEEYKSFKWEYSPIICEPI
                     GGEMYRIYLSALEDGLSEETLNRMFTETAARASGTREGFEEKLRCLLQCCRSGELPFT
                     LAQAEAFLDTYRSQGYPAVRHSSCYRSAYHPAYRIVSASYARYYEAFIRIDRALRERM
                     QVQIAIDGMCAAGKSTMGRILQSIYSCRLFHMDDFFLQPYQRTADRLSQIGGNVDYER
                     FKKEILDHLNDREGLEYQIYSCKAQRLDCRIQTDWTPLSIIEGSYSQHPFFGDIWDLR
                     FFCEIDPAEQLNRIRIRNGEAMCERFKQEWIPKENAYFDTFGIREKSICLTDGKTKNS
                     KNILTD"
BASE COUNT          689 a          820 c          631 g          861 t
ORIGIN      
        1 gatgtcctcc aatgggtcac ggccctccac agaggcagcg tctaccacat gaaccagcac
       61 cttggtacgc tcaatatggc gcaggaagga gtggcccagc cctacgcctt cagaggctcc
      121 ctcgatcagt ccggggatat cggccataac aaaaccggct ccgtctccta agtccactac
      181 acccagatgg ggatttaatg tagtaaaatg ataattggca atttcgggct ttgcattgct
      241 gacaacggaa agcagggtgg attttcctac atttggataa cctacaaggc ctacatctgc
      301 aatgactttg agctccagct gtacccacag ctcctgtccg ggttgtccgg gctgggcata
      361 ctttggcacc tgcatggtag aggtggcaaa gtgcatattt ccaagtccgc ccttgccgcc
      421 ttttaggatt acttcccgtt tgttgtctcc tgacatatcg gcaatcacct tgccggattc
      481 aaaatctttg atcacagttc cttctggaac tttaacgatc agatcatcgg catctgctcc
      541 gtggcagcgg cgttttccgc cttcctgtcc atcctttgcg atgtatttgt gtatatgacg
      601 gaaatcgatc agtgtatttt ttcctgtatc aacctgaaaa atcacatcgc cgcctcttcc
      661 gccgtcaccg ccgtcaggac cgccgttagg aacataaagc tcccgtctga aggacacatg
      721 gccgtcgccg cccttaccgg acttgataaa tacttttgca cgatctgcaa acatataaca
      781 gcacctcctg tatggtgtgg cacacgctcc gtcatccgga acgcatggtt tttgtatgcc
      841 ccgtcttttc gggtacactg tctaaaaaaa gggctccata caccaggtat ggggcccctc
      901 agaattattc gttgattgct cttgggtaaa cagaaacctg ctttctgtct ctgccctttc
      961 tttcaaactt aacaactccg tctactaatg caaataaggt atcgtcgccg cctttgccta
     1021 cgttgttgcc aggatgaatg tgagtaccgc gctgtctgta caggatgttt cctgccagta
     1081 cgaactgtcc atcagctctc ttggcgccca gtctcttgga ctcggaatca cggccgttct
     1141 tagtagaacc aacacccttt ttatgagcga ataactgaag gttcatcttt aacatgtctt
     1201 acacctcctt gaatcggatg ataatatatg ttctcccata atccttctca atattctgaa
     1261 gtcccagaat aagagaattc ataaggagct gcgactcagg acttatatct gaggtaaacc
     1321 gaaagtcaaa ctgaacgtcc tcctgtccgg cagccccctc aaatgggtct tccgtaaatg
     1381 tctctaccga attataaaaa ttgatcgcca gcgccgatac agctgcgcag atgatatcct
     1441 caccctcttc agcatatcca gcatgacctt caatcacaat cccccggtaa agcacattgc
     1501 ctgaatctct ggaatctttt aatacagttg cctgaatcat atcatttaca attaggcatt
     1561 gatcttttcg atcttaacct gtgtatagag ctgtctgtgg ccgttcttct tgtgatagcc
     1621 agtctttctc ttatacttgt aaacgatcac tttcttagcc ttaccttcct tctcaactgt
     1681 tgctgtaaca gttgctccgg ctacggttgg gcatccgatc tgcagttcgc cgttgtttac
     1741 agccagcacc tggtcaaatg ttacggtctc gcctgcgcct gcgcccagtc tttctacctt
     1801 aatgacatcg ccttctgcta ccttgtactg ctttccgcct gttgcaataa tcgcgtacat
     1861 aaaaggcacc tcctatgttc attactcgcc agcttcggtg ccggttttct ggatgcatcc
     1921 attctcccgg acttttaccc cgctgtgcgg catactcgta tactatagca ttcctgtttt
     1981 aatctgtcaa tatgttttta gaattttttg tttttccgtc cgtcaggcag atactttttt
     2041 cacggatgcc gaacgtatca aaatatgcgt tttctttcgg gatccattcc tgcttaaaac
     2101 gttcgcacat tgcctcgccg tttctgatcc ggattctgtt taactgctct gcgggatcaa
     2161 tttcacagaa aaacctcaga tcccagatgt ctccaaagaa ggggtgctgg ctgtaggagc
     2221 cttcaatgat gcttaaggga gtccagtccg tttgtattcg gcagtccagc ctctgagcct
     2281 tacagctgta gatctgatat tccagtccct ctctgtcatt taagtgatct aaaatctctt
     2341 ttttaaaacg ttcataatct acattcccgc cgatctggga cagccggtcg gcggtgcgct
     2401 gataaggctg taaaaaaaag tcatccatat ggaacagccg gcaggagtaa atactttgaa
     2461 gaatccgccc catggtactc ttccctgcgg cgcacatccc gtcaatggca atctgtacct
     2521 gcatcctctc tctcaaagcc ctgtcaatcc ttatgaaagc ttcataatag cgtgcatagc
     2581 tggcggaaac gatccgatag gccggatgat aggcactgcg gtaacagcta ctgtgacgga
     2641 cggcgggata tccctggctg cggtatgtat ccagaaatgc ttctgcctga gccaaagtga
     2701 acggaagctc tcccgaccgg cagcactgga gcagacacct tagtttctcc tcaaagccct
     2761 cccttgttcc ggatgctctg gctgcagttt ctgtaaacat ccggtttaaa gtttcttcgg
     2821 acagtccgtc ctccagagca gacagataaa tccggtacat ttctcccccg attggttcgc
     2881 atataatggg agaatactcc cacttaaagc ttttgtactc ttctttcaga cgatccaggc
     2941 ttttttcagg actggtgatc atatggccgc cgccaaattc gctctgatac agcagcttta
     3001 c
//
LOCUS       DBMDUH010000223         2996 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_11379_length_2996_cov_8.841451, whole genome shotgun sequence.
ACCESSION   DBMDUH010000223 DBMDUH010000000
VERSION     DBMDUH010000223.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11379_length_2996_cov_8.841451"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1350
                     /gene="tilS"
                     /locus_tag="ACZPCG_12820"
     CDS             <1..1350
                     /gene="tilS"
                     /locus_tag="ACZPCG_12820"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HIR1285965.1"
                     /translation="ADSVCLLRILVQLRDILGIRLRAVHVHHGLRGEEADRDSRFAEE
                     LCRELSVPFSLIYADVRGLADREGISEEEAGRILRYESFESEGKKWESEEPGAGTSSV
                     KIAVAHHSGDQAETILHNLFRGSGLGGLKGMPYVRGNVIRPLLDVSGEEIREYLRERG
                     FSWKEDSTNHTDHYTRNRIRREILPAVEKRINPGAGANILRMGKLAAMADSYLRQQAG
                     RWLEIWALDENNGTRSIFLPEKPFKEADAILQLYAVMEVLKELAGSSKDLGLVHGQAA
                     AELFDRQPGRRIDLPYGLEARREYGGIRIGGIKAFSGKNREKEGASEGLDFLPKPVFT
                     VFSYKKGLSIPKNMYTKWFDCDKIKGTPVIRTRQPGDELALSPGVHKPLRRYMIDEKI
                     PSELRDRIPVLADGNRVMWVIGYRISSDYKIDEATKRVFQAELPDSEERKLPAKRKD"
     gene            1368..1898
                     /gene="hpt"
                     /locus_tag="ACZPCG_12825"
     CDS             1368..1898
                     /gene="hpt"
                     /locus_tag="ACZPCG_12825"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HIR1285966.1"
                     /translation="MADKIRVLLTEKEVDERINEVAELINRDYEGKHVHLICILKGGV
                     FFTCELAKRLTVPVSMDFMSVSSYGSGTVSSGIVRIIKDLDEPLEGKHVLIVEDIIDS
                     GQTLAYLIEVLKQRGPADIRLCTLLDKPERRVKKQVKVDYTCFTIPDEFVVGYGLDFD
                     QKYRNLPYIGVVEQED"
     gene            1951..>2996
                     /locus_tag="ACZPCG_12830"
     CDS             1951..>2996
                     /locus_tag="ACZPCG_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HIR1285967.1"
                     /translation="MRQQQTFRGFVFLIMMLVLLAFAFKFTSTAGAGDRLTYQEFLQV
                     LDTEKVEEAVISQNPQAPTGTVTLTLEGDRIARQNVSDVKEAEQLLRDRNIDYAVEGV
                     PQENTMLTVVMPFLLSIVVVIVIITMMNRGAAGNGANARMMNFGRSRARLSRDSKVNF
                     SRVAGLEEEKEELEEVVDFLKNPQKYTSVGARIPKGLLLVGPPGTGKTLLAKAVAGEA
                     GVPFFSISGSDFVEMFVGVGASRVRDLFEEAKKNAPCIVFIDEIDAVARRRGTGMGGG
                     HDEREQTLNQLLVEMDGFGINEGIIVMAATNRVDILDPAILRPGRFDRKVAVGRPDVK
                     GREEILKVHSKEKPL"
BASE COUNT          805 a          603 c          899 g          689 t
ORIGIN      
        1 gcagattcgg tatgcctgct gcggatcctg gttcagttga gagatattct gggaatccgg
       61 ctgagggccg ttcatgtcca tcatgggctg cggggagagg aggcggaccg ggacagccgg
      121 tttgcagagg aactctgccg ggagctttcc gtgccttttt cccttattta tgcagatgta
      181 aggggactgg cggacagaga ggggatttca gaagaggaag caggtcgtat cctgcggtat
      241 gaaagctttg agtcagaagg aaaaaagtgg gaatcagagg agccaggggc aggcacctcc
      301 tccgtgaaaa tagcagtggc ccatcacagc ggggatcagg cagaaaccat tttacataac
      361 ctgtttcgcg gcagcggcct gggagggtta aaggggatgc cttatgtgcg tggaaatgtg
      421 atccgtcctc ttttggatgt aagcggagaa gaaatacggg aatacctgag ggagagaggg
      481 ttttcatgga aggaggattc caccaatcat acagaccatt ataccagaaa ccgcatccgc
      541 agggagatcc ttccggcggt ggagaaacgg atcaatcccg gagccggggc caatatcctg
      601 cgcatgggaa agctggcggc aatggcggac agctatttaa ggcagcaggc aggacggtgg
      661 cttgagatat gggcgttgga tgagaataat ggaaccagga gcatttttct gccggaaaaa
      721 ccgttcaaag aggccgatgc cattttacag ctgtacgcag tgatggaggt tttaaaggag
      781 ctggctggtt catcaaagga tctgggactg gttcacggtc aggctgctgc ggagctgttt
      841 gaccggcagc caggacgccg gatcgatctt ccgtatggtc ttgaggccag gagagaatat
      901 ggggggatac ggattggggg aataaaggca ttttctggga agaacaggga gaaagagggg
      961 gcatcagaag ggctggattt tcttccaaag ccggttttta ctgtattttc ttacaaaaaa
     1021 ggcctttcaa ttcctaaaaa tatgtatacg aaatggtttg actgtgataa aataaagggt
     1081 acgcccgtca tcaggaccag acagccggga gatgagctgg ccctttcgcc aggggttcac
     1141 aagcctctca gaaggtatat gatcgatgaa aaaatccctt ctgagcttcg tgaccggatt
     1201 cctgttctgg cagatggaaa ccgtgtgatg tgggttatcg gataccgcat cagcagcgat
     1261 tacaagatag acgaagcgac aaaaagagta tttcaggcag aacttcccga cagtgaggaa
     1321 aggaagctgc ctgcaaaaag aaaagattaa aaacggagga taaaatcatg gcagataaga
     1381 tccgcgtatt gctcacagag aaggaagtag acgagagaat caatgaggta gcagagctta
     1441 ttaacagaga ttatgaggga aagcacgtcc atctgatctg tattttaaag ggaggcgtgt
     1501 tctttacctg tgaactggcc aagagactga ccgttcccgt atccatggac tttatgtctg
     1561 tatccagcta cggctcaggc actgtatcca gcggtatagt gcgtatcatc aaggatctgg
     1621 atgagccgct ggaggggaaa catgttctca ttgtggagga tattattgat tccggccaga
     1681 cactggcata tctgatcgag gtattaaaac agagaggccc ggcggatatc cgtctgtgca
     1741 cccttcttga caagccggag cgacgtgtga agaagcaggt aaaggtagac tatacctgtt
     1801 ttaccattcc ggatgagttc gtagtgggat acggtctgga ttttgaccag aagtacagaa
     1861 atctgcccta catcggagta gtagagcagg aggactgatg tcctttggta tgtacagagt
     1921 attacatcat ttgattgaag gaggcagaca ttgagacagc agcagacatt cagaggattt
     1981 gtatttctca ttatgatgct ggtactgctg gcctttgcgt ttaaatttac gtctacggca
     2041 ggggcaggag acaggcttac ttatcaggag tttttacagg ttctcgatac ggaaaaggta
     2101 gaggaggcgg taatcagcca gaatccccag gcacctacgg gtacagtaac cctgactctg
     2161 gagggagacc ggatcgcccg tcagaacgtt tcggatgtaa aggaagcaga acagctgctc
     2221 agggaccgca acattgatta tgcagtagaa ggcgtaccgc aggaaaatac catgcttact
     2281 gttgtgatgc cgtttctcct ttctattgtt gtggtaattg tgattattac gatgatgaac
     2341 cgcggggcgg ccggaaacgg agcaaatgcc agaatgatga atttcggcag aagccgcgca
     2401 aggctcagcc gtgacagcaa agtgaacttt tcacgggtag caggacttga ggaggagaag
     2461 gaagaactgg aagaagtagt tgatttcctg aagaatcctc agaaatatac cagcgtgggg
     2521 gccaggatcc ccaagggact tcttcttgta ggccctccgg gaacaggaaa aacccttctt
     2581 gccaaggcag tagccggaga agctggagtt ccgtttttct ctatttccgg ttctgatttt
     2641 gtggaaatgt ttgtaggtgt aggtgcatcc cgtgtccgcg acctttttga agaagctaag
     2701 aagaatgctc cctgtattgt atttatcgac gagatcgatg cagtagcccg acgcagagga
     2761 accggtatgg gcggaggcca tgatgagaga gagcagacct taaaccagct tctggtagag
     2821 atggacggtt ttgggatcaa cgagggaatt attgtcatgg cggctaccaa ccgtgtagat
     2881 atcctggatc ctgcgatcct gcgtcccggc cggtttgacc gcaaggttgc tgtgggacgg
     2941 ccggatgtaa agggaaggga agagatctta aaggttcatt ccaaggaaaa gcccct
//
LOCUS       DBMDUH010000224         2989 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_11414_length_2989_cov_6.791551, whole genome shotgun sequence.
ACCESSION   DBMDUH010000224 DBMDUH010000000
VERSION     DBMDUH010000224.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11414_length_2989_cov_6.791551"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="ACZPCG_12835"
     CDS             complement(<1..542)
                     /locus_tag="ACZPCG_12835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285968.1"
                     /translation="MNTGRKWFIKAAALACAAMLMTPLTAFAGWQKEGDTYAYYEEDG
                     TRVVNDFRRSGNQWFYLDENGHILKNCQREINGKLYTFNERGAMDSSPSRRSGEGSKS
                     SVSGSSFTAISSRQQYLNKTANPYMNNQTVQWFNAACAILTRHNAGNIRAFGGGLKLA
                     GKEENGEEMDRAAKDQNRQM"
     gene            complement(669..1817)
                     /gene="nagA"
                     /locus_tag="ACZPCG_12840"
     CDS             complement(669..1817)
                     /gene="nagA"
                     /locus_tag="ACZPCG_12840"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006905249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HIR1285969.1"
                     /translation="MSRQLFYNGRVYRDHRFETLCVLSENGVITEIGEWIYAPEAERI
                     DLKGRRLVPGFLDIHTHGAAGVDVNSADPDGYEKISRFMASQGTTGWLGSVLTDTREQ
                     TLTYIEAYKQWKDMEHKGAAMMGIHLEGPFLSPEYKGAMPEHLLQSKNIPLIEEYQKA
                     AQGEIRYITVSPEVDGMVEDIPKLKALGIQVAIGHSGADYDTARAAVANGAMASTHTG
                     NAMKLLHQHFPAIWGAVLEDDRVYCEVICDGRHLHPGTVRLILKTKGLERVVAVTDSI
                     MAAGLPDGEYRLGVNEVVVIDGDAKLKENGTRAGSTLTSGKALKNLIAFTGRPAEELI
                     PLLTENPAKLLGMDGQRGFIRQGLFADFVVLDENDGVEETYVQGRRVF"
     gene            2031..>2989
                     /locus_tag="ACZPCG_12845"
     CDS             2031..>2989
                     /locus_tag="ACZPCG_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012740131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1285970.1"
                     /translation="MALHVMDEASRCLGCKVPQCQKGCPISTPIPQVIRLLKEGKLDE
                     AGQMLFENNPLTTVCSLVCNHENQCEGHCVLGRKGAPVHFSTIESYISTTYANKMTKG
                     PAPSNGIRAAIVGSGPAGLTIAVILARYGYDVTIFEGKDQIGGVLRYGIPEFRLPKSV
                     LDDFKYRHLDLKGIKFRPNTHIGGAIGIDDLFRDGYKAIFVGTGVWKPNALHIKGETL
                     GHVHFGINYLNNPDSYCLGERVIVIGAGNAAMDVARTAIRKGVRYLTCFSITKKVAAS
                     HHEFSYAQLEGVQFEYNKAPVEITDEGVIFKDMAEDEEGRLTE"
BASE COUNT          746 a          808 c          646 g          789 t
ORIGIN      
        1 aacatctgac gattctgatc ttttgccgcc ctatccatct cctcgccgtt ctcttccttt
       61 cccgcaagct ttaagccacc gccaaaggca cggatattgc cggcattgtg ccgggtaaga
      121 atcgcacagg cagcattaaa ccactgaact gtctgattgt tcatataggg atttgctgtt
      181 ttgtttaaat actgctgccg gctgctgata gctgtaaaag aagagcctga tacactgctt
      241 ttactccctt cccctgatct gcgggaagga cttgagtcca tagcgcctct ttcattgaat
      301 gtataaagct ttccgttgat ttccctttga cagttcttta aaatatggcc gttttcatcc
      361 aggtaaaacc actgattgcc tgatctacgg aagtcattga ccacccgagt gccatcctct
      421 tcataatacg cataggtatc tccctctttc tgccagcctg caaaggctgt aaggggagtc
      481 ataagcatcg ccgcgcaggc aagagctgct gcttttataa accattttcg tcctgtattc
      541 atccgaatca tcctctcacc tatctctgct gtgcaccttc tgtctgagca catttacagt
      601 atagcacagg cgtttcggat tgccaactta cacatttctg acgaaattat aataaatgtc
      661 aggaaatttt aaaaaacacg ccgtccctgt acataagttt cttctacccc atcattctca
      721 tccaacacca caaagtctgc gaaaagtccc tggcggataa atcccctctg gccatccatg
      781 cccagaagct ttgccggatt ttctgtgaga agcggaataa gctcctctgc cggccgccct
      841 gtaaaggcaa tcagattttt cagtgctttt cccgaagtca aggtacttcc tgcccgtgta
      901 ccattttctt tcagttttgc atccccgtca atgaccacaa cctcattaac gcccaggcga
      961 tactctccat ccggcagacc cgcagccatg atcgaatcag tcacagctac tacccgctca
     1021 agcccctttg tctttaagat caagcgcacc gttcccggat gcagatggcg gccgtcgcag
     1081 atcacctcgc agtaaacacg gtcatcctcc agcaccgctc cccagatggc aggaaaatgc
     1141 tggtggagca gcttcattgc atttcctgta tgggtcgacg ccattgctcc atttgcaacg
     1201 gccgctctgg ccgtatcata atctgcccca gaatggccga tggctacctg gatgccaaga
     1261 gcctttaatt tggggatatc ctctaccatg ccgtctacct caggcgaaac cgtaatgtaa
     1321 cgtatttccc cctgggcagc cttctggtat tcctcgatca aaggaatatt tttactctgc
     1381 agcagatgct ccggcatagc gcccttatac tctgggctta aaaatggacc ctccagatgg
     1441 atccccatca tggcggcgcc tttgtgttcc atgtccttcc actgtttata tgcttcaata
     1501 taggtcagtg tctgctccct cgtatctgtg agcacagaac ccagccagcc agttgtaccc
     1561 tgagatgcca taaagcggct gatcttctca tatccatcag ggtctgcgct gtttacatcc
     1621 accccagcgg caccatgggt atggatatct aaaaatcccg gcaccagacg gcgtcccttt
     1681 aaatcaatgc gctctgcctc aggagcataa atccattctc cgatctcggt gatcacacca
     1741 ttttcagaaa gaacacagag cgtttcaaac cgatgatccc tgtatacacg tccattgtaa
     1801 aataactgtc tgctcataat gatacctctc ccgttcttat atttttattt ctgtcttcat
     1861 agtaacacag accggggata ctttgtgaaa cttttaccca aacgtttgtg tcatgctttt
     1921 aagattgtgc attttttatc attcgtatca gaatcagata catgccatta tgcactggca
     1981 acagattttc ttttatgata agatagaaaa aaagtagaaa ggaaatcaat atggcattac
     2041 atgttatgga tgaagccagc cgctgccttg gctgcaaagt tccccagtgt caaaagggct
     2101 gccccatcag cactccgatt ccccaggtga tccgcctctt aaaggaaggg aaattagatg
     2161 aggccgggca gatgctcttt gaaaataatc cgctgaccac tgtgtgcagt ctggtctgca
     2221 accatgaaaa tcagtgcgag ggccactgcg ttttgggacg caagggtgct ccggtccatt
     2281 tttcaaccat tgaaagctat atttccacca cctatgccaa caaaatgaca aaggggccgg
     2341 ctccatcaaa cggcatccgt gcagcgatcg tcggctccgg ccctgccggc ctgaccatcg
     2401 cagtgatttt ggcacggtac ggctatgatg tgactatctt tgagggtaag gaccagatcg
     2461 gaggcgtact gcgctacggc attcctgaat tccgccttcc aaagtctgtt ctggacgatt
     2521 tcaaataccg ccatctggat ctgaaaggca tcaaattccg gccaaacacc catatcggag
     2581 gggcaatcgg tattgacgac ctcttccgcg acggctataa agctattttc gtaggaaccg
     2641 gtgtatggaa gcccaatgcc cttcatatca aaggcgaaac gctgggacat gtgcactttg
     2701 gaatcaatta tctgaataat cctgacagtt actgcctggg cgaacgggtt attgtgatcg
     2761 gagcggggaa tgccgctatg gatgtggcaa gaaccgccat ccgaaaaggc gtacgctatc
     2821 tgacctgctt ttccatcaca aaaaaggtgg cagccagcca ccatgaattc agctatgccc
     2881 agctggaagg agttcagttt gaatacaaca aggctcctgt ggaaatcaca gatgaaggag
     2941 taatctttaa ggatatggcc gaggatgaag aaggccgtct caccgaaat
//
LOCUS       DBMDUH010000225         2978 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_11477_length_2978_cov_4.292318, whole genome shotgun sequence.
ACCESSION   DBMDUH010000225 DBMDUH010000000
VERSION     DBMDUH010000225.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11477_length_2978_cov_4.292318"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            55..399
                     /locus_tag="ACZPCG_12850"
     CDS             55..399
                     /locus_tag="ACZPCG_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285971.1"
                     /translation="MELFYHKDQQLIWQNHKLAMIAGIYEEIEKDFHKSYDRKDLQMK
                     AYMDMGVRSRLFETYQNHPGVFTLDQYCYYHIYLLGILCKLDEMTIQENIKDAFAFAD
                     SHPLDIRSCIFE"
     gene            complement(440..1690)
                     /gene="pepT"
                     /locus_tag="ACZPCG_12855"
     CDS             complement(440..1690)
                     /gene="pepT"
                     /locus_tag="ACZPCG_12855"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007037283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HIR1285972.1"
                     /translation="MVQNRSQVTERFLRYAAVDTQSADDMEQMPSTEKQRVLGHMLAE
                     ELKELGVSDVREDENGYVYGVIPSNLEEGHQAPALGFISHMDTAPAFSDTNVKPQIIE
                     NYDGEDICLNKDASTWLSANDFPEMKNYKGKTLITTDGTTLLGADDKAGVAEIMTMAA
                     WFLSHPEEKHGKICIAFTPDEEVGRGADRFDVNGFGADVAYTVDGGAIGELEYENFNA
                     ASGRVKIHGASIHPGSAKGKMKNALLMGMEFQSLLPVFENPMYTEEYEGFFHLDRMEG
                     SVEEASLEYIIRDHNKQKFEDKKELFKEAAAYLNKKYGSGSIEVQVKDSYYNMKEKIE
                     PYMYLIDEAKAAMEELGITPVIVPIRGGTDGARLSYMGLPCPNLCTGGHNFHGKYEYI
                     CAESMETITELLIRIAKRFGEMDR"
     gene            complement(1790..2344)
                     /locus_tag="ACZPCG_12860"
     CDS             complement(1790..2344)
                     /locus_tag="ACZPCG_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009214715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HIR1285973.1"
                     /translation="MGTWNTRGLRGSTLEDLINRTNDSYREKKLALIQKIPTPITPIQ
                     IDKSSRHITLAYFDQKSTVDYIGAVQGIPVCFDAKECSASTFPLQNIHAHQIRFMEEF
                     EAQGGIAFIVLYYTGMDEIYYVPFRTIKHFWDRMEAGGRKSFTYDEIDHSWRIRAHRD
                     MLVHYLEMIARDLELREGLEDAET"
     gene            complement(2492..>2978)
                     /locus_tag="ACZPCG_12865"
     CDS             complement(2492..>2978)
                     /locus_tag="ACZPCG_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285974.1"
                     /translation="IKIVALNPYFRGRSVLAPSQDESYDADSINDIATYTSVIDEDRA
                     EWTCDTVGNKSTLHPSTSSLVFEIQGDLNTIVYFKINHKEYKASIKDLLEYGYVTEME
                     YYHSQAFKIHPALPCTRYQFEGEIEDNVPQLSWDVYHMEVCQKNRQWAYVSPVYVKNN
                     E"
BASE COUNT          769 a          746 c          615 g          848 t
ORIGIN      
        1 cactgcataa tctatatcta cgcctacaga aagataatga gcacgcctaa aaacatggaa
       61 ttattttatc ataaggatca gcagctgatc tggcagaatc ataagctggc catgatcgcc
      121 ggaatctatg aagagattga aaaggacttc cacaaatcct atgaccgaaa agatctgcag
      181 atgaaggcat acatggatat gggggttcga tccagactgt ttgagaccta ccagaatcat
      241 cccggcgtat ttaccctgga tcaatactgc tactatcata tctatctgtt gggcatttta
      301 tgcaagctgg atgaaatgac catccaggaa aacataaaag acgcctttgc ctttgccgat
      361 tcccatcctc tcgatatacg gtcatgtatt tttgaataaa gcgccccatt ccgaagatcc
      421 ggaagggacg catttgtttt tatcgatcca tttccccgaa tcgctttgca atccggatca
      481 gaagctctgt aatggtttcc atagattctg cacagatata ctcatacttc ccatggaaat
      541 tgtggcctcc ggtacacagg ttggggcagg gaaggcccat ataggaaagc cttgctccgt
      601 ctgtgccgcc gcggatgggt acgatcaccg gagtgatacc cagctcttcc atagctgcct
      661 ttgcctcgtc gatcaggtac atatagggct cgatcttctc tttcatgttg tagtaggaat
      721 cttttacctg tacctctata gaaccagagc cgtatttttt gttcagatag gcagctgctt
      781 ccttaaacag ttccttttta tcctcaaatt tctgcttatt gtggtcacgg ataatgtatt
      841 ccagagaagc ctcctctaca ctgccctcca tccggtccag atggaagaag ccttcatact
      901 cctctgtata catgggattt tcaaagaccg gcagcaggga ctgaaattcc attcccatta
      961 aaagggcgtt tttcatcttt cccttagcgc tgccgggatg gatgctggct ccatggatct
     1021 ttacccgccc ggatgccgca ttaaaattct catattccag ttctccgatc gctccgccgt
     1081 ccacggtgta ggccacatct gctccaaagc catttacatc aaagcggtca gcgccccgtc
     1141 caacctcttc atccggggta aaggcgatac agatcttgcc atgcttttcc tccggatggg
     1201 ataaaaacca ggctgccatg gtcatgatct ctgccacacc cgccttatca tctgccccta
     1261 aaagggtggt gccgtcagtg gtgataagcg tctttccctt ataatttttc atttccggaa
     1321 agtcatttgc ggaaagccag gtggaggcgt ccttatttaa acagatatcc tctccatcgt
     1381 aattttcaat aatctgaggc tttacgtttg tgtctgaaaa tgcaggcgct gtatccatat
     1441 gggaaataaa acccagagca ggtgcctgat ggccttcctc cagatttgag gggatcacgc
     1501 cgtatacata gccgttttcg tcctcccgca catccgatac gcccagctct ttcagttcct
     1561 ctgccagcat atggcctaaa accctttgct tttctgtact tggcatctgc tccatatcat
     1621 ccgcagactg ggtgtctacg gctgcatagc gcagaaagcg ctctgttacc tgtgatcgat
     1681 tctgtaccat tgtgtttcct ccttaaatgt accattttca caaagttcag tttctaaaac
     1741 tgtaaataag tatatcacag gaaccctatc ctgtccaatc cgctttagct tacgtctctg
     1801 catcctccaa accctctctc agctccagat cccttgcgat catttccaga taatgcacca
     1861 gcatatccct gtgagctcgg atccgccagg aatggtcaat ttcatcatag gtaaagctct
     1921 tgcgccctcc ggcttccatc cggtcccaaa agtgctttat ggtacggaaa ggcacataat
     1981 atatctcatc catccccgta tagtacagaa caataaaggc aatccccccc tgagcctcaa
     2041 actcctccat aaaacggatc tggtgagcat ggatgttctg cagaggaaaa gtagatgcac
     2101 tgcattcctt ggcatcaaag catacgggaa tcccctgaac cgctccgata tagtccacgg
     2161 tactcttctg gtcaaaataa gccagagtga tgtgccggct ggacttatca atctggatcg
     2221 gtgtgatggg cgtgggtatt ttctgtatca gggcaagctt tttctccctg tagctgtcat
     2281 tggtgcggtt aatcaaatcc tcaagggtag agccccgaag gcctctggtg ttccaggttc
     2341 ccatcgtgtg tcattttcct ttctgatgtc atgatgctga tgatacacat aaaagtataa
     2401 caggagaatc cactaagagc aaggcgcaaa aaaagcgctt tttcctatag ataatcctgc
     2461 ggaaaaagca ctttttattc atagcaaaca gttattcatt atttttgaca tagaccggtg
     2521 aaacataggc ccattgccga tttttctgac acacttccat atgatataca tcccaggaaa
     2581 gctgtggaac gttatcttca atttccccct caaactggta acgggtacat ggaagagcag
     2641 gatggatttt aaatgcctga gaatgatagt attccatttc tgtcacatag ccatattcca
     2701 gaagatcttt gatagaagct ttatactcct tgtgattgat cttaaaataa acaatcgtgt
     2761 taagatctcc ctgtatctca aacaccagag aagaagtaga gggatggagt gtagatttat
     2821 ttcctaccgt atcacaagtc cattctgccc tgtcttcatc tattacagag gtatatgttg
     2881 ctatatcatt gatactgtca gcatcatagg attcatcctg agaaggcgcc agtacgcttc
     2941 ttccgcggaa atatgggttc agagcaacga tttttatt
//
LOCUS       DBMDUH010000226         2960 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_11559_length_2960_cov_4.720532, whole genome shotgun sequence.
ACCESSION   DBMDUH010000226 DBMDUH010000000
VERSION     DBMDUH010000226.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11559_length_2960_cov_4.720532"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1394)
                     /gene="mutL"
                     /locus_tag="ACZPCG_12870"
     CDS             complement(<1..1394)
                     /gene="mutL"
                     /locus_tag="ACZPCG_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HIR1285975.1"
                     /translation="MAVISVLDQTTINQIAAGEVIERPASVVKELLENAIDAQATAVT
                     VEIREGGCSLIRITDNGCGIPKEEIPTAFLRHATSKIRSADDLTTVASLGFRGEALAS
                     IAAVSQVEMISKTADRLTGTRYQIEGGTERGIEEVGAPEGTTIIARNLFFNTPVRKKF
                     LKTPMTEGTHVAAVVEKIALSHPDISIRLIQNNQNKLYTSGNNNLKDLIYTVFGREIT
                     SNLLSVDAGYEGIHITGFIGKPVIARSNRNYENYFVNGRYVRSNIISKAIEEAYKPYM
                     MQHKYPFTMLHLSIDPETLDVNVHPTKMELRFSDGEYVFNRTLEAVGEALAHKELIPQ
                     VTLDEAAKKRQEDAKKRREERQPRQPEPFEIRRQSSLDAGNLSSGNRSSSSGSNPVFQ
                     ETASAQRSGVSRPSLLCEEAPGWKHVDKKDLPANESAEGEKKTQTVFQTKQTEDAGRA
                     LNPPAAAAPISRSSV"
     gene            complement(1409..1918)
                     /gene="rimM"
                     /locus_tag="ACZPCG_12875"
     CDS             complement(1409..1918)
                     /gene="rimM"
                     /locus_tag="ACZPCG_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567739.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HIR1285976.1"
                     /translation="MEQMLRVGVITSTHGVRGEVKVFPTTDDAKRFKTLKKVILDGRE
                     PLELSIEQVKFFKNMVILKFKGYDNINDVETWRQRDLLITRDQAVELKEDEYFITDLI
                     GLTVVNEEEAVLGRVKDVLETGANDVYVVELTGGKELLLPAIKDCILNVDLEGGRMKV
                     HVLDGLMDL"
     gene            complement(1991..2218)
                     /locus_tag="ACZPCG_12880"
     CDS             complement(1991..2218)
                     /locus_tag="ACZPCG_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HIR1285977.1"
                     /translation="MKELVEVIAKALVDNPDEVVVTESMKGEDTLIELKVAPADMGKV
                     IGKQGRIAKAIRSVVKAAASKEDRKVIVEIQ"
     gene            complement(2247..2489)
                     /gene="rpsP"
                     /locus_tag="ACZPCG_12885"
     CDS             complement(2247..2489)
                     /gene="rpsP"
                     /locus_tag="ACZPCG_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015554336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HIR1285978.1"
                     /translation="MAVKMRLRRMGQKKAPFYRIVVADSRSPRDGRFIEEVGYYDSTK
                     DPSVIKFDEEAAKKWLANGAQPTETVAKLLKIAGIQ"
     gene            complement(2578..>2960)
                     /locus_tag="ACZPCG_12890"
     CDS             complement(2578..>2960)
                     /locus_tag="ACZPCG_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583196.1"
                     /note="with 4.5S RNA forms a signal recognition particle
                     involved in targeting and integration of inner membrane
                     proteins; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HIR1285979.1"
                     /translation="NDFLTQMQQIKKMGGMGSILSMMPGVGSQLSGIDMDEGERSLRR
                     VESIILSMTKEERANPGLINPSRKQRIAKGAGVDVSEVNRLVKQFDQMKKMMKQMPGL
                     MGGGKRKGLGGLGGLMGGKLKLPF"
BASE COUNT          667 a          806 c          617 g          870 t
ORIGIN      
        1 acagaagatc gggagatcgg agcagctgcg gcagggggat tcaaagcccg cccagcatct
       61 tctgtctgtt tcgtttgaaa cactgtctga gtttttttct caccttcagc tgattcattt
      121 gcaggcagat cctttttatc aacatgcttc cagccgggag cttcctcgca gagaagagat
      181 ggccgggaga caccggatct ctgtgcagac gctgtctctt ggaacacagg attagaaccg
      241 gaagatgacg acctgtttcc ggaagacaaa tttcccgcgt ccagactgct ctgccggcgg
      301 atctcaaagg gctccggctg tctgggctgg cgctcctctc tccgcttttt ggcatcctcc
      361 tgccgctttt tggctgcttc atccagcgtc acctgaggaa tcagttcctt gtgtgccagc
      421 gcttctccca ctgcctctaa cgttcggtta aacacatatt ccccatcgct gaagcgcagc
      481 tccatcttgg tggggtgtac atttacatcc agggtttcag gatcgataga aaggtgcagc
      541 atggtaaaag gatatttgtg ctgcatcata tagggcttat aggcctcttc aatggctttt
      601 gaaataatat tgctgcgcac atacctgccg ttgacaaagt aattttcata attccggttg
      661 gatcttgcga tcaccggctt tccgataaag cctgtaatat ggattccctc ataacccgca
      721 tcgacagaaa gcaggttgga agtgatctca cggccaaaca cggtgtagat cagatctttc
      781 agattgttat ttccggaggt atacagctta ttctggttat tttggatcag acggatggat
      841 atatccggat gggaaagagc aatcttttct accactgctg ccacgtgtgt cccctctgtc
      901 attggggttt ttaagaattt tttccgtaca ggcgtattaa aaaagagatt tctggcaatg
      961 atcgtagttc cttccggggc ccccacttct tctatcccgc gctccgtccc gccttcgatc
     1021 tgatatcggg ttccggtaag tctgtctgct gttttggaga tcatctctac ctgggatacg
     1081 gcagcaatac tggccagcgc ctctccacga aagccaaggg acgcaacggt ggtaagatca
     1141 tcggccgaac gtatcttgct ggttgcgtgg cgcagaaagg cggtgggaat ctcctccttt
     1201 gggataccgc agccattgtc cgtaatgcgg atcaggctgc agccgccctc ccggatctct
     1261 acggtcactg ccgtagcctg agcatcaatg gcattttcca aaagctcttt taccacagaa
     1321 gccggtctct cgatcacctc gcctgcggcg atctgattaa tagttgtctg atccagaact
     1381 gaaatcacag ccatatagca aacctccttt acagatccat caagccgtcc agcacatgaa
     1441 ccttcatgcg tccgccttcc agatccacat tcaggatgca gtccttgatt gcaggaagaa
     1501 gaagttcctt tccgccggta agttcaacta catatacgtc atttgcccct gtttccagta
     1561 catccttcac tctgccaaga accgcttctt cttcatttac aaccgtaagg ccgatcagat
     1621 ctgtgataaa gtattcatcc tcttttaatt caaccgcctg atctctggtg atcaaaaggt
     1681 ctctctgtct ccatgtttcc acatcattga tattgtcata tcccttaaat tttaagatta
     1741 ccatattttt aaagaatttt acctgttcga tggaaagctc caaaggctct cttccatcca
     1801 gaattacctt ttttaatgtt ttaaagcgct tggcatcatc cgttgtaggg aataccttta
     1861 cttccccccg tacgccgtgg gtggaagtaa ttacgcctac tcgcagcatc tgttccatgt
     1921 ctgaacactc actttctctt tatctttaac gaaacagacc gccggccaaa cagccgacgg
     1981 tccgtcctct ttactggatc tcaacaatta ctttcctgtc ttccttagag gcagcggcct
     2041 tgactacgga acggattgcc ttagcgatcc tgccttgctt gccaatgacc ttgcccatat
     2101 cggcaggagc taccttgagt tcgatcaatg tatcttcgcc tttcattgac tcggtaacga
     2161 ctacttcgtc cggattatcg accaatgcct ttgcgatcac ttcgactaat tccttcatct
     2221 ctccacctcg tctgcaaaga ataatattac tgaataccag cgatctttaa cagctttgca
     2281 actgtctcag ttggctgagc gccgtttgcc agccacttct tagcagcttc ctcatcaaac
     2341 ttgatcacgc ttggatcttt ggtagaatca tagtatccaa cttcctcgat aaatcttccg
     2401 tctcttgggg atctggaatc tgctactacg attctataaa aaggagcctt cttctgtccc
     2461 attcttctta atctcatctt tactgccatt tttcttacct ccgtaaaatc attttctatt
     2521 gattgtttta tttttctata ttagacatac aggagccttc ctgtgtgact agcttattta
     2581 aaacggaagc ttaagcttcc cgcccataag gccaccaaga cctcctaagc cctttcgctt
     2641 tccgccgccc ataaggcccg gcatctgctt catcatcttc ttcatctggt caaactgctt
     2701 taccagacgg ttcacttcgc tgacatctac tccggcaccc tttgcgatgc gctgtttcct
     2761 agaaggattt ataaggcccg gattggcccg ctcctctttt gtcatggaga ggataatgga
     2821 ttccacacgg cgcagggagc gctcgccttc atccatatca atgccggaaa gctggcttcc
     2881 aacaccgggc atcatggaaa gaatacttcc cataccgccc atctttttaa tctgctgcat
     2941 ctgggttaag aagtcattgt
//
LOCUS       DBMDUH010000227         2800 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_12345_length_2800_cov_5.299741, whole genome shotgun sequence.
ACCESSION   DBMDUH010000227 DBMDUH010000000
VERSION     DBMDUH010000227.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12345_length_2800_cov_5.299741"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2457)
                     /locus_tag="ACZPCG_12895"
     CDS             complement(<1..2457)
                     /locus_tag="ACZPCG_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR1285980.1"
                     /translation="MIQPKRTYGDKIMETLEKIGLSEQEREKIFSYVSGERGEEALQG
                     LTARDFYQLAETQLEAAAALFSHFCDRHSEEAKKMLRVLFTLGQSTCGALERGNYAWR
                     YSFMREGKDFWGLKPEEWLSVVAEIAGRNNDTRFSYLMDQMIKQTKTPEYLLSAMKYC
                     IGDMANGRLILLSLYFLRKYPDKKLEEPKEGAAGAKGILNRLVSAFGNGNGLSGEDAE
                     LMRQYETIVCNTIEDALPSSVSKASRFQIARALKEGSMTDEIRSLAGPLAQNNLITWL
                     ICGTAFMNMALSPCLMRTVDLYLASDMNCCLPIMEQLDFRRDLHERGGSFDELFHLDS
                     KSYISWAAIRKQGKILCSQLKKNKELYLECLKEADLEQMNYMMNVVRSADPKLYKTLQ
                     SSAGMRRRDAVIKVLTEEKGPETQTLIDYLYGRVPFSALYPYEEQMAKNSHFYRGYRL
                     RNLLDGFPAKDEDREFFNRCQACTFLRQEGYFMSSGKNPFYQPKEEAFAGFFKDMEEA
                     GMDEAHLLKTFILNVKSLYLDSDKERCFELAARRVFLYKLRANPEAVMEVFRESEAEG
                     RRLGLMTMALMAKEYKKEILSYAKDTAKSVREELLKILEEQKDWKDDILVLLSDKKAG
                     PREAAVYVLAAWNDVSDRPALEAALEKEKNAKVRRLLTQVLGVKEEENESPSGVLKRE
                     DLVKALHKGGKSRTLSWAFQTPFPAVHFENGEEASVEYLQAILLCYAGAGTSPGVNKD
                     AEFLAGDLKKEEFSLYVNELFDRFMEQGAEAKKKWVLYAASIHGGNLMVKKLYRQIQE
                     WPANARGAMACEAVNALALSP"
     gene            complement(2501..>2800)
                     /locus_tag="ACZPCG_12900"
     CDS             complement(2501..>2800)
                     /locus_tag="ACZPCG_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285981.1"
                     /translation="YRLLEEVSQQIEDLYQSGFDAVHDSTLGMLRQWAERAGESGLAF
                     LGIQLGKLCREIEGCRHSLHPVHGAELEIYVNIAEYLWLARRKTEFDLAEAYYTQ"
BASE COUNT          647 a          802 c          594 g          757 t
ORIGIN      
        1 agggcttaag gccagtgcgt tgaccgcctc acaggccatg gcgcctctgg catttgccgg
       61 ccattcctgg atctgacggt acagcttttt aaccatcaga ttgcctccgt ggatggacgc
      121 tgcataaaga acccatttct tctttgcttc tgcgccctgc tccataaaac ggtcaaagag
      181 ttcatttaca taaagagaaa actcctcttt cttaaggtct ccggccagaa attcagcgtc
      241 tttatttacg cccggactgg ttcctgctcc ggcataacac aggagaatgg cctgaaggta
      301 ttcaacggac gcctcctccc cgttttcaaa atgaaccgcg gggaaaggag tctgaaaggc
      361 ccaggacagc gtgcggctct ttcctccttt atgaagggct tttaccagat cctccctctt
      421 taaaacgccg gaggggcttt cattttcttc ctcctttacc cccagcacct gggttaaaag
      481 ccggcgcacc ttggcatttt tttccttttc aagagctgcc tcaagagccg ggcggtcgga
      541 aacgtcattc catgcagcca aaacatatac cgcagcttcc ctggggcctg cctttttatc
      601 gcttaaaaga acaagtatat catccttcca gtccttctgc tcctctaaaa tttttaaaag
      661 ctcctcccgg acggatttag ccgtatcctt tgcataggaa agaatttctt ttttgtactc
      721 ttttgccatc agagccatgg tcataagccc cagacggcgg ccttctgcct cagactcacg
      781 aaacacttcc atcactgctt ccggattggc tctgagctta tacagaaata ctcttctggc
      841 tgccagctca aagcagcgtt ccttatccga atcaaggtat aaggatttta cattcaggat
      901 aaaggtcttt aacagatgcg cctcatccat tcccgcttct tccatgtctt taaaaaatcc
      961 tgcaaaggct tcttccttcg gctggtagaa cggatttttt cccgaggaca taaagtaccc
     1021 ctcctgccga agaaaggtac aggcctggca gcggttaaaa aattcccggt cctcatcctt
     1081 agcaggaaag ccgtccagca gattacgcag tctatagccc ctgtaaaaat ggctgttttt
     1141 tgccatctgt tcctcatatg gatacagagc gctgaaagga acccgtccat acaggtaatc
     1201 aattaacgtc tgggtttccg ggcctttttc ctctgtcagt acctttataa ccgcatctct
     1261 tctgcgcatg ccggcagagg actgaagggt tttataaagc ttaggatctg cgctgcgaac
     1321 cacattcatc atgtaattca tctgctccag atctgcctcc tttaggcatt ccagatacag
     1381 ctccttattc tttttaagct ggctgcaaag aatcttcccc tgctttctga tggctgccca
     1441 ggaaatgtag gacttgctgt ccagatggaa cagctcatca aagcttcctc cccgttcgtg
     1501 aagatccctg cggaaatcca gctgctccat aataggaagg cagcagttca tatcagaagc
     1561 cagatacaga tccactgtgc gcatcagaca gggggacagg gccatattca taaaagcggt
     1621 tccgcatatg agccatgtaa taagattatt ctgggccaga ggacctgcca gagaacggat
     1681 ctcatccgtc atggatcctt cctttagcgc acgggcgatc tgaaaccggc tcgccttgga
     1741 aacagaggac ggcagcgcgt cttcaatggt gttgcataca atcgtttcat attgcctcat
     1801 aagctccgcg tcttcaccgg aaagcccgtt tccatttcca aacgctgaaa caagccggtt
     1861 caaaataccc tttgcacccg cagccccctc tttgggttct tccagctttt tatccgggta
     1921 tttccggaga aaatagaggg aaagaaggat cagcctgcca ttagccatat ccccgatgca
     1981 atacttcatg gctgacagca ggtattccgg agtcttagtc tgttttatca tctgatccat
     2041 cagatagctg aaacgtgtat cattgtttct gcccgcgatc tctgccacaa ccgaaagcca
     2101 ctcttccggc ttcagtcccc agaagtcctt cccctctctc ataaagctgt aacgccatgc
     2161 ataattcccc cgttccagcg cgccgcaggt tgactgtccc agtgtaaaaa gcacccggag
     2221 catttttttt gcttcctctg aatgccggtc gcagaaatgg ctaaacagag ctgccgcagc
     2281 ctccagctgc gtctcggcaa gctggtaaaa gtcccttgcc gttaagccct gtaaggcttc
     2341 ctctccccgt tcgcctgata cataggaaaa aatcttctcc cgttcctgtt cagacagacc
     2401 gattttctcc agagtttcca ttattttatc cccatatgtt ctctttggct gtatcatacg
     2461 ccttctcctc ctttttaccg gatttttgct gtatcgtcta tcactgtgta tagtaggctt
     2521 ctgccagatc aaactccgtc tttcgtctgg ccagccacag gtattccgct atatttacat
     2581 aaatttccag ttctgcccca tgaacgggat gcaggctgtg acggcagcct tcaatctccc
     2641 ggcacagctt cccaagctgt atgccaagaa aggcaagccc gctctctcct gcacgttccg
     2701 cccattgccg aagcatccca agggtgctgt catgaacggc gtcaaacccg ctctggtaca
     2761 gatcctcgat ctgctggctg acctcttcca gaaggcggta
//
LOCUS       DBMDUH010000228         2669 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_13083_length_2669_cov_4.846573, whole genome shotgun sequence.
ACCESSION   DBMDUH010000228 DBMDUH010000000
VERSION     DBMDUH010000228.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13083_length_2669_cov_4.846573"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            18..113
                     /locus_tag="ACZPCG_12905"
     CDS             18..113
                     /locus_tag="ACZPCG_12905"
                     /inference="COORDINATES: protein motif:HMM:NF019371.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HIR1285982.1"
                     /translation="MKPDELKTILQVLTLYRNACAHDERLYNVWS"
     gene            385..1014
                     /locus_tag="ACZPCG_12910"
     CDS             385..1014
                     /locus_tag="ACZPCG_12910"
                     /inference="COORDINATES: protein motif:HMM:NF016763.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HIR1285983.1"
                     /translation="MDQLYCIYRSIEKSICSTISASFPSACIVSFLAFISYMVFRLFF
                     SLHKNRSKNFSGVSDMVYQGVWTAIFIFYTYMVLFRTWIARKRWEDPLDKIFGGWGLY
                     TEKGNLTTEAIENVMLLFPFTFLLGILLRKRWKKYSPGSAFRYLALSGFFMSLTIETV
                     QLLLKRGTFQFSDLFYNTTGGILGGAFYLTIRRLRFKICACKWQSKHKK"
     gene            1057..>2669
                     /gene="glmS"
                     /locus_tag="ACZPCG_12915"
     CDS             1057..>2669
                     /gene="glmS"
                     /locus_tag="ACZPCG_12915"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HIR1285984.1"
                     /translation="MCGIVGFIGNHQAAPILLDGLSKLEYRGYDSAGLAVRDGDAPVQ
                     IVKAKGRLKALAEKTNDGEALKGTCGIGHTRWATHGEPSETNAHPHCSDDGNVVGVHN
                     GIIENYQELRDKLVKKGYTFYSSTDTEVAVKLIDYYYKKYEGTPVDAINHSMVRIRGS
                     YALAVMFKEYPGEIYVARKDSPMILGVTDGECYVASDVPAILKYTRNVYYIGNMEMAR
                     LKEGKITFYNLDGDEITREMTQIEWNAEAAEKGGFEHFMMKEIHEQPKAVSDTLNSVL
                     KNGRLDLTEMGLTEEEICAISQIYIVACGSAYHVGVVAQYVMEDLAEIPVRVDVASEF
                     RYRKPLLDKNGLVIVVSQSGETADSLAALREAKEYGVKTLAIVNVIGSSIAREADHVF
                     YTMAGPEIAVATTKAYSTQLIAVYCLALQFALVRKKLSEEKCAALIAELQSLPGKMEK
                     ILEDKERIQWFASKYAMAKDAFFIGRGIDYAVSLEGSLKLKEISYIHSEAYAAGELKH
                     GTISLIEDGTLVIGVLTQSQLYEKTLSNMA"
BASE COUNT          763 a          493 c          712 g          701 t
ORIGIN      
        1 tcggtcgttt cttttctttg aagccagatg aactgaaaac tatattacag gtgttgacac
       61 tctaccgtaa cgcatgtgct catgacgaac gtctctacaa tgtgtggtct tagcccattt
      121 tgctgcctgc tatgtagcaa aacatgaaat gtgctacatg ataagcagca gtcaaatcgt
      181 cacgcatact ctataatata aatgtagaaa aaactgcaca tggaaaggaa cgatgatgac
      241 gagcaaagga gaaaaaagtt agaaaaagga gtgtacagag ctgatttatt ttccgacttg
      301 tgatatattg aatctgttct gcgtatattt tatgagaaaa agaagaaatt cagcagaaat
      361 atttttacaa gggatcatac atggatggat caactatact gtatttaccg aagcattgaa
      421 aaaagtattt gctcaacaat ttctgcatcc tttccatcgg catgtattgt ttcttttctg
      481 gcatttataa gctatatggt gttccggctg ttttttagtc tgcataaaaa cagatcaaaa
      541 aatttttcgg gtgtttccga tatggtttat cagggcgttt ggactgcgat atttatcttt
      601 tacacatata tggtattgtt tcgtacctgg attgcaagga aacgttggga agatccgtta
      661 gacaagatat ttggaggctg gggcttatat accgaaaaag gaaaccttac cacagaggcg
      721 attgaaaatg tgatgcttct tttcccgttt acgtttcttt tggggatact tttgagaaaa
      781 cggtggaaaa agtattcgcc gggatcagct ttccggtatt tggcactcag cggtttcttc
      841 atgtctttga ccattgaaac agtgcagctg ttactgaaac gcggaacatt ccagttttca
      901 gatcttttct ataatacgac aggtgggata ttaggaggag ctttctattt gacaatacga
      961 aggctgaggt ttaaaatatg cgcatgcaaa tggcagtcaa aacataaaaa atgaaagctc
     1021 aggatgcttt tcagtgtcaa ttaaggagaa agaaatatgt gtggaatcgt tggatttata
     1081 ggaaatcatc aggcggcccc gatccttctg gatggattat caaaactgga atacagaggc
     1141 tatgattcgg caggtctggc tgtccgggat ggagacgccc cggttcagat cgtaaaagca
     1201 aaagggcgcc tgaaggcgct ggcagagaag acgaatgacg gagaggcctt aaagggcacc
     1261 tgcggtatcg ggcacaccag atgggcgact catggagagc cttcggaaac caacgctcat
     1321 cctcactgct cggatgatgg aaatgtagta ggcgtccaca acggaattat tgagaattat
     1381 caggaactgc gtgataagct ggtaaaaaaa ggatatacct tttattcttc aaccgatacg
     1441 gaggtcgctg taaagctgat tgactattac tataaaaagt atgagggaac tcccgttgat
     1501 gcgatcaatc attctatggt gcggattcgg ggatcctatg ctctggcagt gatgtttaag
     1561 gagtatccgg gagaaattta tgtggccaga aaggacagcc ccatgatcct gggagtcacg
     1621 gacggcgagt gctatgtggc ttctgatgtg cctgcgattt taaaatatac gagaaatgta
     1681 tattatatcg gaaatatgga gatggcccgc ctgaaggaag ggaagattac cttttataac
     1741 ctggatggag atgagataac cagggagatg acgcagatcg aatggaatgc agaagcggct
     1801 gaaaagggcg gttttgagca tttcatgatg aaggagatcc atgagcagcc gaaagcagtt
     1861 tcagataccc tgaattctgt tttgaaaaac ggccgtctgg atctgacgga gatggggctg
     1921 acagaagaag agatctgcgc catatcccag atttatattg tggcctgcgg ctctgcgtat
     1981 catgtggggg tggtggctca gtatgtgatg gaggatctgg cagagatccc ggtccgtgtg
     2041 gatgtggctt ccgaattccg ttacagaaaa cctcttctgg ataaaaatgg gctggtgatc
     2101 gtagtgagcc agtccggaga gacggcggac agtctggccg ctctcaggga agcgaaggaa
     2161 tatggggtaa agacccttgc gattgtaaat gtgatcggtt cttccattgc cagagaggca
     2221 gaccatgtgt tttacaccat ggcgggaccg gagattgccg ttgcaacgac aaaggcctac
     2281 agcacccagc tgatcgccgt ctactgtctg gctctccagt ttgccctggt gaggaaaaag
     2341 ctttcagagg aaaagtgtgc agcgctgatc gcagagctgc agtctctgcc tgggaagatg
     2401 gaaaaaatcc tggaggataa ggaacggatc cagtggtttg catcgaaata cgcaatggca
     2461 aaggatgcgt tctttattgg aagagggatt gattacgcag tttctctgga gggaagcctg
     2521 aagctgaagg agatttccta tattcattcg gaggcttatg cagcagggga gttaaagcac
     2581 ggaaccataa gcctgatcga ggacggtacg ctggtgatcg gagtgctcac acagagccag
     2641 ctttatgaaa aaacgctgtc caatatggc
//
LOCUS       DBMDUH010000229         2551 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_13848_length_2551_cov_6.397959, whole genome shotgun sequence.
ACCESSION   DBMDUH010000229 DBMDUH010000000
VERSION     DBMDUH010000229.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13848_length_2551_cov_6.397959"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="ACZPCG_12920"
     CDS             complement(<1..700)
                     /locus_tag="ACZPCG_12920"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR1285985.1"
                     /translation="MEIPYTHGGDIYSRPIKMDYSANINPLGLPEGVKRELLCCVKEN
                     VCCIYPDSACRSLKMALSEIHHVPKEWISCGNGAADLIFALVAALRPRHALLLAPSFL
                     EYSQALHLWGCETDSFMLREDEDFSLDPFRLKEALFRARAEKQPYDILFLCNPNNPTG
                     LPVKKEIVEEIGVVCKETGTWLAVDECFCDFLDEPEAYSAIPKLKDLDHVIVLKAFTK
                     IYAMAGLRLGYSLCA"
     gene            complement(709..1707)
                     /gene="cbiB"
                     /locus_tag="ACZPCG_12925"
     CDS             complement(709..1707)
                     /gene="cbiB"
                     /locus_tag="ACZPCG_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HIR1285986.1"
                     /translation="MSLFHLWKFHSMSLIAGCILDWLMGDPYWLFHPVRFMGNMISAL
                     EARLRRLFPEHLLLAGGVLAGFMCLFWTLIPVLGFLGIYRGLLSFGIQRHWIYICLFV
                     LESFFCGQLLAARSLQTESMKVCSALKEGDIEKARKAVSMIVGRDTAVLDRDGIARAA
                     VETVAENTSDGVIAPFFFMAVFGPAGGFFYKAVNTMDSMVGYKNETYLLFGRAAAKLD
                     DAVNWLPARLSGMLLTAAAWLLPGMDGKNAWRIFKRDRFNHASPNSAQGEAACAGALH
                     LRLAGDAWYFGTLYKKPYIGDDDRPIRPDDIKGVCSLMFGAQGLLMAGLVILLLLL"
     gene            complement(1736..2200)
                     /locus_tag="ACZPCG_12930"
     CDS             complement(1736..2200)
                     /locus_tag="ACZPCG_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006426280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HIR1285987.1"
                     /translation="MILITGGRAQGKRAFWEKNIASGEGEPSEISGLWIRGGQTSFDE
                     CLDSPYVCEFHLFIRRLLLGEPSLNAPDWVYGTMEKRNGCRLPDREALTERLFKACPG
                     RVLVTDEIGLGIVPLDPFEREYREETGRICCLLAARSEQVWRVACGLGQRLK"
     gene            complement(2205..>2551)
                     /locus_tag="ACZPCG_12935"
     CDS             complement(2205..>2551)
                     /locus_tag="ACZPCG_12935"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HIR1285988.1"
                     /translation="SGLSVLSFPSAKKDGLAASFAQAAEKKTDRMGMMLWPLATGAVS
                     WLLGGLSWLLAAAVFFVLLLILFVWFYRWSKKQFGGITGDLAGFYLQAGELVCLIAAL
                     VLAGWVSNNVGG"
BASE COUNT          668 a          743 c          553 g          587 t
ORIGIN      
        1 ctgcacagag gctgtagccc agccgcagcc cggccatggc ataaattttg gtaaatgcct
       61 ttaagacaat cacatgatcc agatccttta atttagggat tgctgaatat gcctcaggct
      121 catccagaaa atcacagaag cattcatcca cagccagcca ggttcctgtc tctttgcaca
      181 ccactccgat ttcttccact atttcttttt ttacaggaag ccccgtaggg ttattgggat
      241 tgcataaaaa caggatatcg tatggctgtt tctctgccct ggcgcgaaac agcgcttcct
      301 tcaacctgaa tgggtccagc gaaaagtcct catcttccct cagcataaat gagtctgttt
      361 cacaccccca cagatgaagg gcctggctgt attccagaaa agagggcgct aaaagcagcg
      421 cgtgcctggg ccttaaggct gccaccagtg caaaaatcag atcggccgcc ccgtttccgc
      481 agctaatcca ctcctttgga acatgatgta tttctgataa agccattttt aagcttcggc
      541 aggcgctgtc cggataaatg cagcatacat tttccttcac gcagcaaagc agctctctct
      601 ttaccccttc cggaagaccc agagggttaa tgtttgcaga ataatccatt tttatggggc
      661 ggctgtagat atcgccccca tgagtataag gaatttccat aagctctcct agagtaacaa
      721 aagtaatatc acaaggcctg ccatcaaaag tccctgagct ccaaacataa ggctgcagac
      781 tcctttgatg tcgtctggac ggataggccg gtcatcatcg ccgatataag gctttttata
      841 aagagttcca aagtaccagg cgtccccggc cagacgcaga tgaagagctc cggcacaggc
      901 cgcctctccc tgggcggaat tggggctggc atggttaaaa cggtcccgtt taaagattct
      961 ccaggcattt ttcccatcca ttcccggaag aagccaggcg gcggcggtaa ggagcatccc
     1021 ggaaaggcgg gctggaagcc agtttacagc gtcatccagc tttgccgccg cccttccgaa
     1081 caaaaggtac gtttcatttt tatatcccac catggaatcc atggtattga ctgctttata
     1141 gaaaaatcct cctgccgggc caaataccgc cataaagaaa aaaggcgcaa tcactccgtc
     1201 cgaagtattt tctgccaccg tctccaccgc cgctctggcg attccgtccc ggtcaagaac
     1261 cgctgtatcc cttcctacga tcatggatac ggcttttctg gctttttcta tatccccttc
     1321 tttcagagcc gaacagacct tcatgctctc agtctgtaag cttcgcgccg ccagaagctg
     1381 tccgcaaaaa aagctttcca gaacaaacag gcagatatag atccagtgtc tctgaatgcc
     1441 aaaggacaga agacctctgt atatccccaa aaagcccagt acaggaatca gagtccagaa
     1501 caggcacatg aagccagcaa gcacccctcc tgcaagcaaa agatgctcag gaaacaagcg
     1561 ccgaagcctc gcttcaagcg cgcttatcat atttcccata aaccggaccg ggtgaaacag
     1621 ccagtaggga tctcccatca gccagtctaa aatacagcct gcaatgagag acatgctgtg
     1681 aaatttccat aggtggaaca aactcatttc ttaacattcc tctttctgtt catcgttatt
     1741 tcagcctctg tcccaggccg caggccacgc gccatacctg ctcagaccgg gcagccaaaa
     1801 gacagcagat ccgcccggtt tcctccctgt attccctttc aaagggatct aagggtacaa
     1861 tgccaaggcc gatctcatct gttaccagaa cacggccggg acaggcttta aaaagacgtt
     1921 ccgtcaacgc ctcccgatcc ggcagccggc agccgtttcg tttttccata gtgccgtaca
     1981 cccagtcggg tgcatttaaa gaaggctctc ccaacagcag cctgcggata aaaagatgga
     2041 attcgcagac ataggggctg tccaaacact catcaaaact ggtctgcccc cctcggatcc
     2101 agagtcctga gatttctgat ggttctcctt ccccagaagc tatgtttttc tcccaaaagg
     2161 cccgttttcc ctgagcccgt cctcctgtaa tcaggatcat tttctcatcc tcccacatta
     2221 tttgaaaccc agccggcgag aaccagtgct gcgatcagac agaccagctc tcctgcctga
     2281 aggtaaaacc cggccagatc tcccgtaatc cctccaaact gctttttgct ccaccggtaa
     2341 aaccagacaa acaggatcaa aagcagtaca aaaaatacgg ctgctgccaa aagccagctt
     2401 aaacctccca aaagccagga cactgcacct gtggctaacg gccacagcat cattcccata
     2461 cggtctgttt tcttctctgc agcctgtgca aaagaggctg ccaggccgtc ttttttggca
     2521 gaaggaaagg aaagcaccga caggccgctg a
//
LOCUS       DBMDUH010000230         2472 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_14453_length_2472_cov_4.527204, whole genome shotgun sequence.
ACCESSION   DBMDUH010000230 DBMDUH010000000
VERSION     DBMDUH010000230.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14453_length_2472_cov_4.527204"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1278
                     /locus_tag="ACZPCG_12940"
     CDS             <1..1278
                     /locus_tag="ACZPCG_12940"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase family protein"
                     /protein_id="HIR1285989.1"
                     /translation="EEYVNRKIPIQKRSVSTDDAIELFHDHHMYDKERLFRYRRVSRV
                     NMYSIDGFEDYYYGYMVPDTGYLKYFDLKRYQYGFVLLLPTMGKPKELPEFVPQDKLF
                     ATLAESARWGRGTDLETVGALNDTIAAGGMAHLILIQEALQEKKIAEIAAQIAEKKTA
                     KFVMIAGPSSSGKTTFSHRLSIQLEAAGLRPHPIAVDNYFVNRADSPRDAEGNYDYES
                     IKCLDVEQFNRDMTALMAGERVELPYYNFKKGIREYKGDFLQLGKDDILVIEGIHCLN
                     DELSYALPRESKFKIYISALTQLNIDEHNRIPTTDGRLIRRMVRDARTRGSSARETIR
                     MWPSVRRGEEQNIFPYQEEADAMFNSALIYELAVLKQYVEPLLFGIPRDCDEYVEAKR
                     LLKFLDYFIGVSSEDIPKNSILREFIGGSCLDV"
     gene            1356..2228
                     /gene="lgt"
                     /locus_tag="ACZPCG_12945"
     CDS             1356..2228
                     /gene="lgt"
                     /locus_tag="ACZPCG_12945"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HIR1285990.1"
                     /translation="MEAIGADLSFVNLGITFTHLPNSISVFGFRIAFYGVIIGLGMLA
                     GMAVAFSDAKRRGQDPDLYLDFALYAIVFSIIGARLYYVAFEWDNYKNDLLQIFNLRA
                     GGLAIYGGVIGAVITLIVFTKVRHQSFFSMADTGVLGLLTGQIIGRWGNFFNCEAFGG
                     YTDSLFAMRIKESIVNPSMISQELIDHRIVENGISYIQVHPTFLYESVWNLSVLLFML
                     WYRKRKRFDGEMLWIYFLGYGVGRFWIESLRTDQLKVPGTALAVSQLLSAALVGAAVG
                     ALTYHYKKISKKEI"
BASE COUNT          641 a          507 c          666 g          658 t
ORIGIN      
        1 gaagaatatg taaatcggaa aattcccatc caaaagcgca gtgtatccac ggatgatgcg
       61 atcgagctgt ttcacgatca tcatatgtat gataaagagc gactgttccg ttaccgccgt
      121 gtctcccgtg tgaacatgta cagcattgac ggctttgagg attactatta cgggtatatg
      181 gtgccggata cgggatatct gaaatatttt gatttaaagc ggtatcagta tggctttgtc
      241 ctccttcttc cgacaatggg aaaaccaaaa gaactgcctg agtttgtacc ccaggataag
      301 ctgtttgcca cgctggccga gtccgcccgg tggggcaggg gaacggatct ggagaccgta
      361 ggagctttaa atgataccat cgccgcagga gggatggctc atctgatttt aattcaggag
      421 gcgcttcagg aaaagaagat cgcggagatc gcggcgcaga tcgcggagaa gaaaactgcg
      481 aaatttgtta tgatcgcagg cccgtcctct tctggaaaga ccaccttttc ccatcgtttg
      541 tctattcagc ttgaggctgc cgggctgcgt ccccatccca ttgcggtaga caattatttt
      601 gtaaaccggg cagacagtcc aagggatgcg gagggaaatt atgactatga gagcattaaa
      661 tgcctggatg tagagcagtt taaccgggat atgacggctc tgatggcagg agaacgggta
      721 gaacttccgt attataattt caaaaagggc atacgggagt ataagggtga ttttctgcag
      781 ctgggaaagg acgatatcct tgtgatcgag ggcatccact gcctcaatga cgagctgtcc
      841 tatgcgcttc cgagagaaag caaatttaag atttatatca gtgcattaac ccagttaaat
      901 attgacgagc acaaccggat tcctaccacg gacgggcggc tgatccgccg catggtccgg
      961 gatgccagga cacggggctc ttctgccaga gaaaccatcc gtatgtggcc ttctgttcgc
     1021 agaggagagg aacagaacat tttcccctat caggaggagg cggatgccat gtttaactcc
     1081 gctcttatct atgaactggc agtattaaag cagtatgtgg agccgctgct ttttggaatc
     1141 ccaagagact gtgatgagta tgtggaagca aaacggctgc tgaaatttct ggattatttt
     1201 atcggggtca gcagtgagga tataccgaaa aattccattc ttcgggaatt tatcggggga
     1261 agctgtctgg atgtataagg ggtacggaca gaatatgaca tcagagggca gaaaaaatac
     1321 agacttgtag ctctgccggg aaggaagtat ataatatgga agcaattgga gcagatctca
     1381 gctttgtaaa ccttgggatc acctttaccc acctgccaaa cagcatttcc gtttttggat
     1441 ttcggattgc tttttacggg gtcatcatcg ggcttgggat gttagccggc atggcggtgg
     1501 cattttcaga tgcaaagcgc agaggacagg atccggatct ctatctggat tttgcccttt
     1561 acgccattgt gttttccatt ataggggcca gactttacta tgtggccttt gagtgggaca
     1621 actataaaaa tgatcttctt cagatcttta atcttcgggc aggaggtctg gcaatctacg
     1681 gcggagtgat tggtgcggta attacgctta ttgtatttac caaagtacgc catcagtcct
     1741 ttttctccat ggcagatacg ggagttttag gactgctgac aggacagatt atcggccggt
     1801 gggggaattt ctttaactgc gaggcctttg gaggatatac agacagtctg tttgccatgc
     1861 gcattaagga aagcatcgta aacccgtcca tgatctcaca ggagctgatc gaccacagga
     1921 ttgtggaaaa tgggatatcc tatatccagg tacatcccac ctttttatac gaatcggtgt
     1981 ggaatctgag cgtccttctc tttatgctat ggtacagaaa acgcaagcgt tttgacgggg
     2041 aaatgctctg gatctacttt ttgggctacg gcgtgggacg tttctggatc gagagcctgc
     2101 ggacagatca gctgaaggtt ccgggaaccg ctctggctgt gtcgcagctt ctttccgctg
     2161 ctttggtggg agcggcagtg ggagctttga cctatcatta taaaaaaata agcaaaaaag
     2221 aaatataaaa tccatatatg gatcaaaaga ggcagacagg aggcgaaagc cgttgtctgt
     2281 ctctcgtttt ttccagaaaa ttctttctct tttttgaaag aaaaacgtaa aaacttcttg
     2341 ccaattccca gtttctgtac tattatggac atacaagtgg tagaaagtgg tgcaaagtgg
     2401 tagaggatgg ttgttgcgtg gatgaacggg ggatttggga ttttcctccg gccgcgctga
     2461 tttgccatgg ga
//
LOCUS       DBMDUH010000231         2415 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_14932_length_2415_cov_7.133103, whole genome shotgun sequence.
ACCESSION   DBMDUH010000231 DBMDUH010000000
VERSION     DBMDUH010000231.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14932_length_2415_cov_7.133103"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1169)
                     /gene="glmM"
                     /locus_tag="ACZPCG_12950"
     CDS             complement(<1..1169)
                     /gene="glmM"
                     /locus_tag="ACZPCG_12950"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HIR1285991.1"
                     /translation="MGKYFGTDGFRGEANVDLTVEHAYKVGRFLGWYYGQKNPGERCR
                     VVIGKDTRRSSYMFEYSLVSGLTASGADVFLLHVTTTPSVSYVVRTEGFHCGIMISAS
                     HNPYYDNGIKVINEKGEKLEEAVITEIERYLDGKMEELPLAKRDMIGRTVDFAAGRNR
                     YIGYLISIATRSFKNRKVALDCANGSASAIAKNVFDALGAQTFVINNEPNGLNINTDC
                     GSTHIEHLQKFVAEQGCDAGFAYDGDADRCIAVDNEGNVVDGDRIMYICGKYMKEQGS
                     LFNNTVVTTVMSNFGLYKAFDREGISYEKTAVGDKYVYENMAATGNCLGGEQSGHIIF
                     SKHATTGDGILTSLKVMEVMLEKKQSLAKLASEVEIYPQVLKNVRVKDKIAAQED"
     gene            complement(1231..2091)
                     /gene="rfbD"
                     /locus_tag="ACZPCG_12955"
     CDS             complement(1231..2091)
                     /gene="rfbD"
                     /locus_tag="ACZPCG_12955"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HIR1285992.1"
                     /translation="MRVLVTGVKGQLGHDVMDELAAKGIEGIGVDVEEMDITDADACQ
                     RVIKEAKPDAVIHCAAYTAVDAAEDNVELCRKVNADGTRNIAKVCKELDIKMMYISTD
                     YVFNGQGERPWEPDDHREPLNVYGLTKYEGEIAVEQNVEKFFTVRIAWVFGINGKNFI
                     KTMLRLGKERGAVSVVDDQIGSPTYTYDLAKLLVEMIQTDRYGRYHATNEGLCSWYEF
                     ACEIFKQAGMDVKVSPVDSSSFPAKAKRPSNSRMSKEKLTENGFDRLPSWQDALGRYL
                     KELKKEGQEI"
     gene            complement(2206..>2415)
                     /locus_tag="ACZPCG_12960"
     CDS             complement(2206..>2415)
                     /locus_tag="ACZPCG_12960"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HIR1285993.1"
                     /translation="FVTDRPGHDMRYAIDPTKIETELGWKPKYNFDTGIEQTIQWYLD
                     NQDWWKNILSGEYSSYFDKMYGNRL"
BASE COUNT          550 a          676 c          468 g          721 t
ORIGIN      
        1 tcatcctcct gagccgcaat tttgtccttt actcttacat ttttaagaac ctgaggatag
       61 atttccacct cagaagccag ctttgccaga gactgcttct tttccagcat gacttccatt
      121 acctttaagg atgtcaggat accgtctcct gtggtcgcat gcttgctgaa aataatatgg
      181 ccggactgtt cgcctcccag gcagtttccc gtagccgcca tgttttcata cacatactta
      241 tcgcctactg ctgtcttttc ataggaaatc ccttctctgt caaaggcttt gtacagtccg
      301 aaattagaca taacggtcgt aacaaccgta ttgttaaaca ggcttccctg ctctttcatg
      361 tattttccgc aaatatacat aatgcggtcg ccgtctacta catttccctc attgtccaca
      421 gcaatacagc ggtccgcatc accgtcatag gcaaaaccgg catcgcagcc ctgctctgct
      481 acaaattttt gaaggtgttc aatatgggta gagccgcagt ctgtgttgat gttcaggcca
      541 ttgggctcat tgtttatcac aaaggtctga gcgcccagag cgtcaaatac attctttgca
      601 atggcagaag cgcttccgtt ggcacagtcc aatgccacct tcctgttttt aaaggaacgt
      661 gttgcaatcg agatcagata tccgatatag cggtttcttc cggctgcaaa atccacagta
      721 cgtccaatca tatcccgctt tgcaagagga agctcctcca tcttcccgtc cagatagcgc
      781 tcaatctccg taatcactgc ttcttccagc ttctctccct tttcattgat gaccttaata
      841 ccattgtcat aataaggatt gtgactggca gaaatcatga tgccgcagtg aaagccctct
      901 gtgcgcacca cataggaaac gcttggagtc gttgttacat gaaggagaaa tacgtctgct
      961 ccggaagctg taagaccgga taccagagag tattcaaaca tataactgct tctgcgggta
     1021 tcttttccga tcacaacccg gcagcgctct cccggatttt tctgtccgta gtaccagcct
     1081 aaaaagcggc ccaccttata tgcgtgctct actgtaagat ccacattggc ttcgcctcga
     1141 aagccatctg ttccaaaata ttttcccatt tcttttatgt cctctctgtc tcagattttc
     1201 cctgcatcat gcaggctgcc ttccccgata ctaaatctcc tgtccttcct tcttaagctc
     1261 ctttaaatat cgtcccagag catcctgcca ggagggaagg cggtcaaagc cattctctgt
     1321 gagcttttct ttgctcatac ggctgtttga tggtctcttt gccttagccg gaaagctgga
     1381 ggaatccacc ggacttacct ttacatccat accagcctgc ttaaagatct cgcaggcaaa
     1441 ttcataccag gaacacagtc cctcattggt agcatggtag cggccgtaac ggtcagtctg
     1501 tatcatttcc accagaagct ttgcaagatc ataggtatag gtaggagaac cgatctggtc
     1561 gtccactaca ctcaccgctc cccgttcctt tcccaggcgc agcatggttt tgatgaaatt
     1621 ctttccgtta atgccgaata cccaggcaat gcggacagta aagaatttct ccacattctg
     1681 ctctactgca atctcaccct catattttgt cagtccatag acatttaaag gctctctgtg
     1741 gtcatccggc tcccagggcc gttctccctg tccgttaaac acataatctg tactgatgta
     1801 catcatcttg atatccagct ctttgcagac ctttgcaata tttctggttc cgtctgcatt
     1861 gaccttccgg cacagctcca cattgtcttc cgccgcatct actgcagtat atgctgcaca
     1921 gtggatcact gcgtcaggtt ttgcttcctt tatcactctc tggcaggcat ctgcgtctgt
     1981 gatatccatt tcctccacat ccacgccaat gccttcgatt ccctttgctg ccagctcatc
     2041 catcacatca tggcctaact gtccctttac acctgtcacc agaactctca tatacttaac
     2101 tcctcattct tttagaatct ctactatggc acaaaggctg gcagcggcaa atgtactctc
     2161 tttaagtgcc tttgccgccg tcagccatac gtttgtttct cttatttaca ggcggtttcc
     2221 gtacatttta tcaaaatagc tgctgtactc gccgcttaaa atatttttcc accagtcctg
     2281 attgtccagg taccactgga tggtctgttc aatgcctgtg tcaaagttat actttggctt
     2341 ccagccaagc tctgtctcga tcttggtggg gtcaatggca tagcgcatat cgtgacccgg
     2401 acggtctgta acgaa
//
LOCUS       DBMDUH010000232         2397 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_15096_length_2397_cov_4.736063, whole genome shotgun sequence.
ACCESSION   DBMDUH010000232 DBMDUH010000000
VERSION     DBMDUH010000232.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15096_length_2397_cov_4.736063"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="ACZPCG_12965"
     CDS             <1..444
                     /locus_tag="ACZPCG_12965"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF015384.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HIR1285994.1"
                     /translation="GALMLLGGTASVFGAVCWLRGLTASLLQRKALCRVTLRQGSRMY
                     VAAGLIDTGNRLKEPVSGRPVHVAVQEVIEAVSPRVRGVTYIPYRSVGGEGFLPAVTL
                     DEIKVEQQGNCYTLTRPLVAVVKQPLSPKGEYEVLIQEFDGGAGR"
     gene            481..1221
                     /gene="sigE"
                     /locus_tag="ACZPCG_12970"
     CDS             481..1221
                     /gene="sigE"
                     /locus_tag="ACZPCG_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HIR1285995.1"
                     /translation="MIVKVSIPNRFQFKTAPGFRTMLMQKQGEVHYIGGADILPPPLE
                     TEEEGRMIGLLGSSEDHQARSCLIEHNLRLVVYIAKKFDNTSVGVEDLISIGTIGLIK
                     AINTFKPDKNIKLATYASRCIENEILMYLRRNSKTRLEVSIDEPLNVDWDGNELLLSD
                     ILGTDEDVICQGIEDEVEKDLLNTAISHLEPRERKIVELRYGLADEDGEEMTQKEVAD
                     LLGISQSYISRLEKKIMKRLKKEIVRFE"
     gene            complement(1308..2291)
                     /locus_tag="ACZPCG_12975"
     CDS             complement(1308..2291)
                     /locus_tag="ACZPCG_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HIR1285996.1"
                     /translation="MEQNERRFAVLIDADNVSPKYIKYILDEVSDIGIATYKRIYGDW
                     TDNEKRAWKNVLLDWSVNPIQQYSYTTGKNATDSAMIIDAMDILYSGNVDGFCLVSSD
                     SDFTKLAQRLREAGMFVMGIGEQKTPKPFRAACDTFKLLEIISSEDAAETAPTNKGRA
                     NALTTSKTEVPSIDEIQKTITGIDEIQKAITKLLIENNSQNQPIILARVGNFLTKRFS
                     DFDVRNYGYSKLSTFLESLNNSDFQVVKLHGGYFVQEKSASTSKADIEKEIIRLIQNN
                     KGHVDNLSIIHEELKKKFPDFDVKQYGFSRISSFIRSFGTFKIKDNMVQLR"
BASE COUNT          630 a          499 c          641 g          627 t
ORIGIN      
        1 ggagcgctta tgctccttgg aggcacagca tccgtttttg gggcggtctg ctggcttaga
       61 ggacttacag cgtccctgct gcaaagaaag gccctgtgca gagtaaccct gcgccagggc
      121 agcaggatgt atgtggcggc ggggctgatc gacacgggaa accggctgaa ggagccggta
      181 agcggcaggc cggttcatgt agctgttcag gaagtgatcg aagccgtatc gcccagggta
      241 cggggagtga cttatatacc ctaccgcagt gtgggaggag aagggttcct tccggcagtg
      301 acattagatg aaataaaggt ggagcagcag gggaactgct atacacttac gaggcctttg
      361 gtggctgtgg taaaacagcc tctttctcct aaaggagaat atgaggtcct gatacaggag
      421 tttgacggcg gcgccggaag ataggggaat cgcggcgcca taaaaacagg aggaagtaaa
      481 atgattgtta aagtatccat acccaaccgt tttcagttta aaacggctcc gggttttcgc
      541 acgatgctga tgcagaagca gggggaggtt cattatatcg gaggcgctga tattcttccg
      601 ccgcctcttg agacagagga agaagggcgc atgatcggtc ttttgggaag ctcagaagat
      661 catcaggccc gatcctgtct gatcgaacac aatctgcgtc tggtggtata tattgcaaag
      721 aaatttgata ataccagcgt aggagtggag gatctgattt ccattggcac aatcggcctt
      781 ataaaggcga tcaatacctt taagccggat aaaaatataa agctggctac ctatgcttcc
      841 cgatgtattg aaaatgagat ccttatgtac ctaaggcgca acagcaagac gcgtctggag
      901 gtttccatcg atgagccgtt aaatgtagac tgggatggaa atgaacttct gctctcagat
      961 atccttggaa cggatgaaga tgtgatctgc cagggaattg aggatgaggt ggagaaggat
     1021 cttttaaata cagcgatcag ccatttagag cccagagaac gcaaaattgt agaactgcgt
     1081 tacggcctgg cagatgagga cggcgaagaa atgactcaga aagaagtggc cgatcttctc
     1141 ggcatctctc agtcctacat atccagactg gagaaaaaaa tcatgaagag actgaaaaag
     1201 gagattgtcc gtttcgagta gatgactgga gaactgaatg aatataaaaa tacgccttag
     1261 attccgtaaa aagggaaact aaggcgtatt tttcactgta aaggcatcta ccgcagctgt
     1321 accatattat ccttgatctt aaaggttcca aagcttcgga taaagctgga aatccggctg
     1381 aatccatact gttttacatc aaaatccggg aatttctttt tcagctcttc gtggatgatg
     1441 ctcagattat ctacatgccc tttgttgttc tggatcagac ggatgatctc tttttcgata
     1501 tcagccttgg aggtagaggc ggatttttcc tggacaaagt agccgccgtg gagctttacc
     1561 acctgaaaat cactgttatt tagactttct aagaaggtgg aaagtttgga atatccataa
     1621 ttgcgcacat caaaatcaga gaagcgtttg gtgaggaaat ttcctacccg tgccagaatg
     1681 ataggctgat tctggctgtt gttttcaatc agaagttttg taatggcttt ttggatttca
     1741 tcaataccgg tgatggtttt ctggatctca tcaatggagg gaacctcggt tttggaagtt
     1801 gtaagggcat ttgcacgtcc tttgtttgtc ggagcagtct ccgctgcatc ttccgaagaa
     1861 atgatttcca gaagcttaaa ggtatcgcag gcggcccgga agggctttgg ggttttctgc
     1921 tccccgatgc ccatgacaaa cataccggcc tctctgagcc tctgggccag cttggtaaag
     1981 tccgaatcgc tggataccag gcagaagccg tctacatttc ccgaatacag aatatccatg
     2041 gcgtcgatga tcatggcgga atcggtggca tttttgccgg tggtgtagct gtactgctgg
     2101 atgggattta cggaccagtc caggagcaca tttttccacg cccgtttttc attgtccgtc
     2161 cagtcgccgt aaatgcgttt ataggtggcg ataccaatat cggatacctc atccagaata
     2221 tatttgatat atttgggaga tacgttgtct gcgtcgatca atactgcaaa tctgcgttca
     2281 ttctgttcca tatgcttgtg ttctgtcgct ttcctagaat tatcccaagg gggactgtta
     2341 aacctgcaat cagtgtatca tattcgggca gcaggcacaa gggggacaga agtttca
//
LOCUS       DBMDUH010000233         2394 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_15120_length_2394_cov_8.645443, whole genome shotgun sequence.
ACCESSION   DBMDUH010000233 DBMDUH010000000
VERSION     DBMDUH010000233.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15120_length_2394_cov_8.645443"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            744..1823
                     /locus_tag="ACZPCG_12980"
     CDS             744..1823
                     /locus_tag="ACZPCG_12980"
                     /inference="COORDINATES: protein motif:HMM:NF012307.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HIR1285997.1"
                     /translation="MRKGKTKRKDFQMAEENLDAWVKKIQEIILNTKPDGWQTDPQKR
                     FKPVKHNPVIIKEFGKTRVVYVPTMVELWIQHVIVMILEPIIAGSSYPMSFSSFPGRG
                     SLKGQRAIRRWIESGKGIRNFAQADIRHFYSHIQYKIVRKKLERRVKDNFFLHLIDVC
                     MTYFPKEMPLGFYLSQWLANFMLQELDYDIKCKLKIAHHVRYMDNYTLADDNKKKLHQ
                     ALLYIRQVLGKMRLRMKSDWQVFRFEYTKKNGKKTGRCVSAMGWLFYRSKVLIRKRIL
                     LHVERIARKLHKKEENGQRFPLGLCRGFVSLLGWITHSETYDWYLIHIKELVNVRKIK
                     RIISKMTREVNRHAGMEKRTLQRAA"
     gene            1783..2115
                     /locus_tag="ACZPCG_12985"
     CDS             1783..2115
                     /locus_tag="ACZPCG_12985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1285998.1"
                     /translation="MQEWKKELCREQPETLQELGKGTYIQRRNITPYERKDGNGEADK
                     GYSCEYRILTKEEYFAALEQENSNKNMLTSMAAQADIYEKLIETEKNQLVIMQAIADL
                     YEKENGGV"
     gene            2117..2230
                     /locus_tag="ACZPCG_12990"
     CDS             2117..2230
                     /locus_tag="ACZPCG_12990"
                     /inference="COORDINATES: protein motif:HMM:NF040910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1375 family protein"
                     /protein_id="HIR1285999.1"
                     /translation="MLELYVTLVKAEIRTLDSVPAAFREKVKAAIEAEKQE"
BASE COUNT          727 a          456 c          632 g          579 t
ORIGIN      
        1 tttggagcga ttccgtcaga ggaaattgag ggaacttcgg caactggcta ttgtgatcat
       61 acttgggtaa acgccacaat cacggcggtt tcgctacggt ttggcggttg caatgacggt
      121 cttcgtgacg gcctctgggc ccgcacgctg aacgatgtgg cggctcacgc ctggtggcac
      181 tacggcgcgt ccttacttct tccagcacct gcagcagcgt gagctgctgt tagggggtat
      241 gggggtcttc ccccataaag aaaatagggg gttcggggga tttcccccga tgcccttgca
      301 gggaataaac gaattttagg ggttaccgga acaccctttc cggcggtttc gctacggttc
      361 ggcaattgca atgacggtct taatgacggc ctctgggccc gcacactgaa caatgtggct
      421 gctaacgcct ggtggaacta cggcgcgtcc tttttctatc aattttagaa cgacaaccaa
      481 aatgttcccg gtaatcctac accgcagacg attgaaacat cgttatatcc gcccttatgg
      541 gttttggtga gtggaaatag ttccggtcag gagctgcaag tagcaggaaa tgtccgaaag
      601 cggcgaggag atagaagatc atgcgtatat tgaaatacga tattgaaaag gtgaccggga
      661 ttccctggaa gaaaaagaaa agttatagat acctgtatcg gctggcctgc cgggaagatg
      721 tgattaaaaa ggcttttaaa cgtatgagaa aagggaaaac caagagaaaa gactttcaaa
      781 tggcggagga aaatctggat gcctgggtta agaaaatcca ggaaattatc ctgaatacga
      841 aaccggatgg atggcagact gatccccaaa aacgatttaa gccagtaaag cataacccgg
      901 taattattaa agagtttgga aagacgaggg ttgtttatgt accgactatg gtggaactct
      961 ggattcagca tgttattgta atgatcctgg agccgatcat agcgggaagc agttatccga
     1021 tgagcttctc atcgtttccc ggaaggggtt ccctgaaggg gcagcgagct atcagacgtt
     1081 ggatcgaaag tggaaaggga atcaggaact ttgcacaggc agatatccga catttttata
     1141 gccatattca gtataaaatt gttcggaaaa aattagagcg tagagttaaa gataactttt
     1201 tcctacatct gattgatgtc tgcatgacat attttcctaa agaaatgcct ttaggtttct
     1261 atctttctca atggcttgcg aatttcatgc tccaggaact ggattatgac attaaatgta
     1321 agctgaaaat agcacatcat gtccggtata tggataatta tacgctggct gatgataaca
     1381 aaaagaagct gcaccaggca ctgctttaca ttcggcaggt gcttggaaag atgagactac
     1441 gcatgaagag tgactggcag gtgttccgat ttgaatacac caagaaaaat gggaagaaaa
     1501 cggggcgctg cgtgtcggcc atgggctggc tgttttaccg gtcaaaagtg ttgatccgga
     1561 agcgcatatt acttcatgta gagcgcatag cccgaaaatt gcataaaaag gaagaaaacg
     1621 gccagcggtt tccactgggg ctttgccgtg gttttgtatc gttgctgggg tggatcacac
     1681 attcagaaac gtatgactgg tatttgatac atattaagga attggtaaac gtccgtaaaa
     1741 tcaaacggat tatttcaaag atgacaaggg aggtaaatcg tcatgcagga atggaaaaaa
     1801 gaactctgca gagagcagcc tgaaaccctt caggagcttg gaaaaggtac ttacattcag
     1861 agacgcaata tcactccgta tgaaagaaaa gacgggaacg gagaagctga taagggttat
     1921 tcatgtgagt acaggattct tacaaaagaa gaatattttg cagccctgga gcaggaaaac
     1981 agcaataaaa atatgctgac ttccatggcc gctcaggcag atatctatga gaaacttatt
     2041 gagacagaaa aaaatcagtt agtcatcatg caggcaattg cagatctgta tgaaaaagag
     2101 aatggaggcg tgtaatatgt tagaacttta tgtgacctta gttaaagctg aaatcagaac
     2161 ccttgacagc gtaccggcgg ccttccggga aaaggtgaaa gcggctattg aagcggaaaa
     2221 acaggaatga taggaaggga gaagaaacat gactctgctt catattatag acacgctttg
     2281 cgatgtgaca accaagcagg cagatctcct ccgggagctg gtgacagatc tggaacatat
     2341 gaaccaggtt tccgaagaag taaagaatta ttatcgggag aagctggatt ctat
//
LOCUS       DBMDUH010000234         2294 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_15963_length_2294_cov_5.201094, whole genome shotgun sequence.
ACCESSION   DBMDUH010000234 DBMDUH010000000
VERSION     DBMDUH010000234.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15963_length_2294_cov_5.201094"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            158..658
                     /locus_tag="ACZPCG_12995"
     CDS             158..658
                     /locus_tag="ACZPCG_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008722966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIR1286000.1"
                     /translation="MRNISVKKLVTAALFAALICVFTMSIRIPTPGTGGYIHPGDAVV
                     ILSGIFLDPVTAFLAAGIGSCMADLLGGYFIYVPITFAVKGLVAAVTSVVYCHVVSKG
                     KSRKLAVLYGGIADILLVAGGYFLFESVLYGPATALLSLSANGIQGISGLVIAGALYP
                     GLERLL"
     gene            1059..>2294
                     /locus_tag="ACZPCG_13000"
     CDS             1059..>2294
                     /locus_tag="ACZPCG_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002603643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HIR1286001.1"
                     /translation="MKEYLSSIQDVLKEQNTSEEGLSSQEAAGRLEKFGHNKLKEGKK
                     DSLLKKFLDELADPMIIILIVAAVISGITAYYEGESFADVIIIMAVVIINAVLGVVQE
                     SKAEAAIAALQEIAAATSKVLRDGKLVTLKSEELVPGDVVILEAGDSVPADGRIIECA
                     SMKIEEAALTGESVPVDKIIQVLNLGGEKDVPLGDRKNMVYMGSTVVYGRGKAVITAT
                     GMDTEMGKIADALAQAKDEETPLQIKLNQLSKFLTMLVVGICVVIFAVGLFRAQGSAE
                     GITGDTILSTFMVAVSLAVAAIPEGLAAVVTIVLSIGVTNMSRRNAIIRKLTAVETLG
                     CTQIICSDKTGTLTQNKMTVVEHVADDEKLLETAMALCSDASLSPDSGEAVGEPTECA
                     LVNDAAKNGLPKDQLEKEYV"
BASE COUNT          623 a          441 c          649 g          581 t
ORIGIN      
        1 tgtatggaca ggataaaaga catggcaaag acaggaatga aaagataaca ggaaacaaag
       61 aactggactg gtttaaaata acagaaatgg atattttaac cggtccataa ttctgctata
      121 ctacgggaaa ccgtgaaaaa tcaaggagga acgtatgatg cggaatattt ctgtaaaaaa
      181 actggtaaca gcagccctgt ttgcagccct gatctgtgtg tttaccatgt caatccggat
      241 tccaacacca ggtaccggag gatatattca tccgggtgat gccgtggtta ttctgtccgg
      301 gatttttctg gatccggtaa ctgcatttct ggcagccgga attggctcct gtatggcgga
      361 tttactgggc ggatatttta tctatgtacc gattacattt gcagtcaaag gccttgtggc
      421 agctgtcaca tctgtagtat actgtcatgt agtgagtaaa ggaaaaagca ggaagctggc
      481 agttttatat ggtggtatag ctgatatttt gctggttgca ggaggatact ttctttttga
      541 aagcgttctc tatggcccgg caaccgctct tttaagtctc tctgccaatg ggatccaggg
      601 gatcagcgga ctggtgatag cgggagcgct gtatccgggg ttggagaggc tgctttaaat
      661 acagtgagag aacttccgat ggatacggaa tatatggcaa tacgcgggcc attttttaga
      721 ggaacataaa aactcctgaa aatagaatct tgacagtttc ccctgtccgt gatagcatta
      781 tgggcagtaa gggagtagtc agcgcagttg accggcagtt ttcaggccgg gaaccgtgta
      841 gcgaagtcaa cagcatcgga gcgtgtccgt ggcttggttt taaatgacga gacttatcgg
      901 aagtcttctt caatggaagg ggcttctggt aggtctcgtt tttgtttgta ccaatacagg
      961 aaatacgcca gatgtacctg ccggaagatg tatagctgag gaagcgggcg ggcatgatga
     1021 agaaagacac ttccagataa taaaaggagg aaaaagcaat gaaagagtac ctgtccagta
     1081 ttcaggatgt gctaaaggag cagaatacct cagaagaagg actttcttct caggaagctg
     1141 caggccgtct ggaaaaattc gggcataaca agctgaagga ggggaaaaag gattccctgc
     1201 ttaagaagtt tcttgatgaa ctggcagatc ccatgatcat tattctgatc gtggcggctg
     1261 ttatttcagg tattaccgct tactatgagg gcgagtcctt tgcagatgtg atcatcatca
     1321 tggcggtagt tattatcaat gcagttctgg gcgttgttca ggagagcaag gcagaggcgg
     1381 cgattgcagc cctgcaggag attgctgcag ctactagtaa ggtgctgcgc gacggaaagc
     1441 ttgttacatt aaagagtgag gagctggtac ccggtgatgt ggtcatcctg gaagcgggag
     1501 attccgtacc ggcagacgga cgaatcatcg agtgcgccag catgaagatc gaggaagcgg
     1561 ctctgaccgg agagtctgtt ccggtagaca agatcatcca ggtgttaaat ctgggcggag
     1621 aaaaggatgt acctttaggc gatcgtaaaa acatggtata catgggatca accgttgtat
     1681 atggtcgtgg aaaagctgtc atcacagcga caggtatgga tacagaaatg ggtaagattg
     1741 cagatgctct tgctcaggct aaggatgagg aaacgcctct tcagatcaag ctgaatcagc
     1801 tgagtaagtt cctgaccatg ctggtggtgg ggatctgtgt ggttatcttt gcagtaggcc
     1861 tgttccgtgc tcagggaagc gcagaaggca ttacaggcga tacgatctta agtacattta
     1921 tggtagctgt ttctctggca gtagctgcga ttcctgaggg tctggcagcg gttgtcacca
     1981 tcgtgctttc cattggcgtt acaaatatgt cccgcagaaa tgctattatc cgtaagctga
     2041 cagcagtgga aactctggga tgtactcaga ttatctgctc cgacaagacg ggtactctga
     2101 ctcagaataa gatgaccgtt gtggagcatg tggcagatga tgagaagctc ttagaaactg
     2161 ccatggcact ctgctccgat gcttccttaa gccctgacag cggcgaggca gtgggagaac
     2221 ctaccgagtg tgctcttgta aatgatgctg caaagaatgg acttccaaag gatcagctgg
     2281 aaaaggaata tgtc
//
LOCUS       DBMDUH010000235         2236 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_16533_length_2236_cov_5.255738, whole genome shotgun sequence.
ACCESSION   DBMDUH010000235 DBMDUH010000000
VERSION     DBMDUH010000235.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16533_length_2236_cov_5.255738"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            51..1283
                     /locus_tag="ACZPCG_13005"
     CDS             51..1283
                     /locus_tag="ACZPCG_13005"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR1286002.1"
                     /translation="MYTAASGGAAASYEAAAASYEAAALPKEQNDPKLSLHAQSAVLM
                     DGLTGRILYGKNENDIRPMASTTKIMTCILALEAGGLDEFAEASSNAAAQPQVRLGVK
                     KGERYQIRDLLYALMLESYNDAAVMIAEKTAGSTEEFSRRMNEKAESLGCRDTWFITP
                     NGLDKKEEDENGTERIHSTTAADLAQILRYCITQSPQKEEFLKITRTSDYYFTDESGK
                     RAFSCRNHNALLTMMDGALTGKTGFTGGAGYCYTGALFRDGRLYIISLLGCGWPPHKT
                     YKWADARRLFSYGLEQYELTDIFKPEPEHTICTEGGICWGENPEGVLHAVMKREETDS
                     RLEILLNKEEKEQIRREVMLPERIFAPVKKGELLGNIQYSLGDHKLGEYGLYADRDIE
                     ALTVPAAWKHICGMFFIF"
     gene            1432..>2236
                     /locus_tag="ACZPCG_13010"
     CDS             1432..>2236
                     /locus_tag="ACZPCG_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR1286003.1"
                     /translation="MKVKKYKRGLAVMLGLALAVQLPGAVPFHPMDTYAYTGAATVKA
                     SSLNVRSGAGTGYRAIGRLAAGASIQVLGEQRGTDGKTWYQIRFSGTGGTEQTGYVSA
                     EYVRLPVSYTTDSDFEAKLSAEGFPESYKNGLRQLHAQYPNWVFKAKKTGLDWNTVIE
                     NEALLGRNLVSSGSISSWKSVESGAYNWDNSTWTGFDGSNWVAASEDIIRYYMDPRNF
                     LDDTYVFQFLSHEYNSSTQTRDGLAKMVEGSFLSGTTDSTGTGSSTGSTG"
BASE COUNT          619 a          475 c          621 g          521 t
ORIGIN      
        1 cggaaatcgg gcagatacag cggtctggga tcatacagga aaaaggggtt atgtatacgg
       61 ctgcatccgg tggggcagcg gcgtcatatg aggcagcagc ggcgtcatat gaggcagcag
      121 cgctgccaaa agagcagaac gatccaaagc tttccctgca cgcgcagtct gctgttctca
      181 tggacggcct taccggacgg atcctttatg ggaaaaatga gaatgatatc cgccctatgg
      241 ccagtacgac taagattatg acctgtattc tggcattgga ggccggagga ctggatgagt
      301 ttgcagaagc ttcgtctaat gctgcagccc agccacaggt cagactgggg gtaaaaaagg
      361 gggagcgcta tcagatcaga gatctccttt atgctctgat gctggaatcc tataatgatg
      421 cggcagtgat gatcgcagaa aaaacagcag gctccacaga ggaattcagc cgcagaatga
      481 atgaaaaagc tgagagctta ggatgcaggg atacctggtt tatcactccc aatggtcttg
      541 ataaaaagga agaggatgaa aacggtacag agcggatcca ttctaccaca gcggcagacc
      601 tggctcagat cctgcgttac tgcattacac agtctcccca aaaagaggag tttttaaaga
      661 ttaccaggac gtcagattat tattttacag atgaatcagg gaaaagggcc ttttcctgca
      721 gaaaccacaa tgcccttctt accatgatgg acggtgcgct cacaggaaag accggattta
      781 caggaggcgc aggatattgc tatacagggg ctctttttcg ggacggacgc ttgtatatca
      841 tctcgctttt aggttgcggc tggccgcccc ataaaacata taagtgggca gatgccagaa
      901 ggctgttttc atatggtctg gagcaatatg agcttacgga tatttttaaa ccggaaccgg
      961 agcatacgat ctgtacagag ggcgggatct gctggggtga aaatcccgaa ggagttcttc
     1021 atgctgtgat gaagcgggag gaaacggaca gccgtcttga aatcctatta aataaggaag
     1081 aaaaggaaca gatcagacgg gaagttatgc ttccagaacg tatctttgcg cctgtaaaga
     1141 agggagaact cctgggaaac attcagtaca gtctgggtga ccacaagctt ggggagtacg
     1201 gtctgtatgc agacagggat atagaagcgc ttacagtgcc ggcagcctgg aaacatattt
     1261 gcgggatgtt ttttattttt taaacttttc ttacaattca gctgcattat gtgaagtttt
     1321 tgcgttgcag ttgacataga gcaatatttg tcattataat aaagaaaatg ccgtaaaagg
     1381 ggttcttgac acagtctttt atgaaaaaca ggacaggaga tacatataca catgaaggtt
     1441 aaaaaataca aaaggggtct tgcggtaatg ctgggtctgg cacttgctgt ccagcttccg
     1501 ggagcagttc cgtttcatcc gatggatacc tatgcttata cgggagcagc cacagtgaag
     1561 gcctcctctt taaacgttcg cagcggggcc ggaaccggtt atcgggccat aggccgtctg
     1621 gctgcgggag catccattca ggttttggga gaacagcggg ggacggatgg gaagacctgg
     1681 tatcagatcc gtttttccgg taccggaggt acagaacaga cgggatatgt atctgctgaa
     1741 tatgtgcgtc tgcctgtatc ctatacgacg gattcggact ttgaagcaaa actgagcgcg
     1801 gagggctttc cggaaagcta taaaaatggc ctgcgccagc tccatgccca gtatccgaac
     1861 tgggtgttca aggccaaaaa aacagggctg gactggaaca cggtaattga aaatgaagcc
     1921 cttctgggcc ggaatctggt ttcatccggg agcatttcct cctggaaatc cgtagagagc
     1981 ggggcctata actgggataa cagtacatgg accggctttg acggcagcaa ctgggtggca
     2041 gcttctgagg atatcatcag gtactatatg gatcccagaa actttctgga cgatacctat
     2101 gtattccagt tcctttccca tgaatataac tcctctactc agacaagaga cgggcttgcg
     2161 aaaatggtag agggcagctt tctttccggt actacggatt ccacaggaac aggaagcagc
     2221 acagggagta caggcg
//
LOCUS       DBMDUH010000236         2204 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_16848_length_2204_cov_5.278650, whole genome shotgun sequence.
ACCESSION   DBMDUH010000236 DBMDUH010000000
VERSION     DBMDUH010000236.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16848_length_2204_cov_5.278650"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1290
                     /locus_tag="ACZPCG_13015"
     CDS             <1..1290
                     /locus_tag="ACZPCG_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009245190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HIR1286004.1"
                     /translation="ILLPGERESCVGRQNRLENEAAVLQDKIQENEKKKEQLSDKKET
                     YGTVFKLEYGLGYTEDVIEETDDMDGLAKAVCGQLSGSCGNKKQEDVFGSLQEVYHQN
                     RGFLLDYQITLSSLFEDLNDENSFLDMSAKRIDISAKYRGTAVKFKELLQKLCEDADG
                     LAKVLSERDRELFEDILANTISKKIRARIQASKRWVEKMNGLMESMQTSSGLRLSLRW
                     KNKRAEKEEQLDTRALVELLQKDAEIMRPEEIDKISHHFRSKIEEARKLAGDTGAVQS
                     FHATMREVLDYRKWFEFQLECQKTGEKKKELTDRVFFTFSGGEKAMSMYVPLFSAVAA
                     KYAGAREDAPKMISLDEAFAGVDEMNIRDMFRLIVEFEFNFMINSQILWGDYDTVPAL
                     AIYQLIRPENARFVSVIRYLWNGKKKVMVTDQMAQKG"
     gene            1294..>2204
                     /locus_tag="ACZPCG_13020"
     CDS             1294..>2204
                     /locus_tag="ACZPCG_13020"
                     /inference="COORDINATES: protein motif:HMM:NF023224.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02679 domain-containing protein"
                     /protein_id="HIR1286005.1"
                     /translation="MDETGQKKLNECLDYFRQRSVYEKLFQKFKEKYASLGHMGGKAV
                     LSGLTLAEKQDLSGFLQKDYTENKTVTVSAGLLEKCLAESRFSGISGEMLLEAYFGEK
                     LTVKKEEKEKEEKKRKDFFAGLLDGKEEDPVGMWLKEVLENHGNGYEILMQQYRENPE
                     GLRDILKNIMKAYQQICIIRKKQLLAVFAAQVTGNPHYFDAGKTAERLLILFLKDQFK
                     SSFSDEMFPAEEKSCLLYQAGILKDDLSNETLAYGLHAWKQDGCIHKGIEGFLKEKEP
                     VRLTLRTLGELGKVTAQKREVYVVENPA"
BASE COUNT          721 a          369 c          601 g          513 t
ORIGIN      
        1 attcttctgc ccggtgagag agaaagctgt gtaggaaggc agaatcgatt ggaaaatgaa
       61 gcagctgtac tgcaggataa aatacaggaa aatgaaaaga aaaaagaaca gctttcagat
      121 aaaaaagaaa catacggaac cgtgtttaag ctggaatatg gtttgggata tacggaagat
      181 gtaattgagg aaaccgacga tatggacgga ttggcaaagg cagtctgcgg acagctttca
      241 ggaagctgcg gaaataaaaa gcaggaggat gtttttggca gtttgcagga ggtttatcat
      301 cagaaccgtg gatttcttct ggattatcag attacactgt cctctctttt tgaagacctg
      361 aatgatgaaa attccttcct ggatatgagc gcaaagagaa ttgatatttc agcgaaatac
      421 aggggaaccg cagtaaaatt taaggagttg ctgcaaaaac tctgtgaaga tgcggatggg
      481 ctggccaagg ttttaagcga aagagaccgg gaactgtttg aggatattct ggcgaatacc
      541 atcagtaaaa aaatacgtgc ccgaattcag gcaagcaaac gctgggtaga gaaaatgaac
      601 ggactgatgg agtccatgca gacctccagc ggattgagac tgagcctgcg gtggaaaaat
      661 aaacgggcag aaaaagaaga acagctggac accagggctt tggtagaatt actgcaaaaa
      721 gacgccgaaa tcatgcgtcc ggaagaaata gacaaaattt cgcatcattt ccgttccaag
      781 attgaggaag caaggaagct tgcgggagat acaggagcag tgcagtcctt ccatgcgacg
      841 atgcgggagg ttctggacta tcggaaatgg tttgaattcc agctggagtg tcagaaaaca
      901 ggagagaaga agaaagaact tacagaccgg gtgttcttta cgttcagcgg aggcgaaaaa
      961 gccatgtcca tgtatgtacc gctgttttcc gcagtggcag ccaaatatgc gggagcccgt
     1021 gaagacgcgc cgaaaatgat ttctctggat gaggcctttg ccggtgtgga tgaaatgaat
     1081 atccgcgata tgttccgcct tatagtagag tttgaattta attttatgat caattctcag
     1141 attttatggg gggattatga taccgttccc gcattggcaa tctatcagct catccggccg
     1201 gaaaatgcca gatttgtttc tgtgatccgc tatctgtgga atggaaagaa gaaggtaatg
     1261 gtaacagatc agatggctca gaaaggataa aatatggatg aaaccggaca gaaaaagctg
     1321 aacgaatgcc tggattattt ccggcagcga tctgtttatg aaaagctctt tcaaaaattt
     1381 aaagaaaaat atgccagcct gggacatatg ggaggaaaag cagttctgtc cgggcttact
     1441 ttggcagaaa agcaggactt aagcggattc ttacaaaagg attatacaga aaacaaaaca
     1501 gtaaccgttt ccgccggatt gctggaaaaa tgtctcgctg aaagccggtt ttccgggatt
     1561 tccggggaaa tgctccttga ggcttatttt ggagaaaaat tgacggtaaa gaaagaggaa
     1621 aaagagaagg aagagaaaaa aagaaaggat ttctttgccg ggctgctgga tggaaaggaa
     1681 gaagatccgg tgggaatgtg gctgaaagag gttctggaaa atcatggaaa cggctatgaa
     1741 attctgatgc agcagtatcg ggaaaatccg gaaggactga gggatattct gaaaaatatt
     1801 atgaaagcct atcagcagat ctgcattata agaaaaaaac agcttctggc ggtgtttgca
     1861 gcgcaggtaa caggaaatcc ccattatttt gacgccggga aaacagcgga aagacttctg
     1921 attttatttc tgaaagacca gtttaaaagc agcttcagtg atgagatgtt tccggcagaa
     1981 gagaagagct gcctgctgta tcaggcaggt attttaaagg atgatctgtc taatgaaaca
     2041 ctggcttatg gtttgcacgc ctggaaacag gatggatgca tacataaagg aattgaagga
     2101 tttttaaaag agaaggagcc tgttcgattg accctgcgta cccttggaga actgggtaaa
     2161 gttactgcac agaagcggga agtatatgtg gtagaaaatc cggc
//
LOCUS       DBMDUH010000237         2139 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_17496_length_2139_cov_8.177625, whole genome shotgun sequence.
ACCESSION   DBMDUH010000237 DBMDUH010000000
VERSION     DBMDUH010000237.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17496_length_2139_cov_8.177625"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            212..487
                     /locus_tag="ACZPCG_13025"
     CDS             212..487
                     /locus_tag="ACZPCG_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003524638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1286006.1"
                     /translation="MKLKTDDPFVPEDIRAKINGLQSLLDHAADLYSDIYSWYDSELK
                     SYDPKATADDELFDPGTGTVVEGIDYLSIMESLSELQTANECKMQRD"
     gene            complement(484..696)
                     /locus_tag="ACZPCG_13030"
     CDS             complement(484..696)
                     /locus_tag="ACZPCG_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1286007.1"
                     /translation="MYGFSKYDAQDRYDSYNTVQVKLKLNMKTDADILAWVRQHKYGR
                     DTSVQGAIKALIREDIARGQADGTQG"
     gene            complement(742..1947)
                     /locus_tag="ACZPCG_13035"
     CDS             complement(742..1947)
                     /locus_tag="ACZPCG_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_115623313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HIR1286008.1"
                     /translation="MTEGKRNIVHQLLEEYDIQTAEDIQEALKDLLGSTLKEMMEAEM
                     DEHLGYGRSERSDSDDYRNGYKPKRINSSFGSMDIQVPQDRKSTFEPQVVRKRQKDIS
                     GIDQKIISMYAKGMTTRQISDTLMDIYGFEASEGFISDVTDKILPQIEDWQNRPLEEV
                     YPVVYIDAIHYSVREDGVIRKLAAYVILGLTLEGKKEVLSIQIGENESSKYWLCVLNE
                     LKNRGVKDILILCSDGLTGIKEAITAAFPKTEQQRCIVHMVRNTLKYVSDKDRKAFAT
                     DLKTIYHAANEEKALEALEKVTEKWTPKYPNSMKRWHDNWDVVSPIFKFSMEVRKVIY
                     TTNAIESLNSTYRKLNRQRSVFPGSTSLLKALYLATFEATKKWTMPIRNWGQVYGELS
                     IMYEGRLPE"
BASE COUNT          466 a          565 c          517 g          591 t
ORIGIN      
        1 gcttcgggca ggtcaattct gccgacaaag cgagagtatg aaaaaaagtc tgtaaattga
       61 gatcttctat gataagttaa tgagaaaggt aggtggatgt cgtgaccacc tacctttact
      121 atacggatgc ttttggattc tgtcaagatg tattggaaaa tcccatcaaa ttatggtaca
      181 cttcttacag gaaaacatca ggaggtgtgc catgaagcta aaaacagacg atccctttgt
      241 acctgaagat atcagggcaa agatcaatgg actgcagtcc ctgctggatc atgcagccga
      301 cttatacagt gatatctata gctggtatga cagtgaactg aaatcctatg acccgaaagc
      361 caccgcagac gatgaactgt ttgaccctgg cacagggact gttgtggagg ggatcgatta
      421 cctgtctatc atggaatccc tgtcagagct gcagacagcc aacgaatgta aaatgcagag
      481 ggattaacct tgtgtcccat cggcctggcc tctggcgatg tcttcccgta tcagggcctt
      541 gatggcccct tggacggaag tgtccctgcc atatttgtgc tgtctgaccc aggccaggat
      601 atctgcatct gtcttcatat tcagttttaa cttcacctgt acagtattat agctgtcgta
      661 cctgtcctga gcatcgtatt ttgaaaatcc atacatggaa agctcctttc cggataggtg
      721 tacacctatg cctgtgcgct cttattccgg cagtctgcct tcgtacataa tggacagttc
      781 cccatagacc tgcccccagt tgcggatggg catggtccac ttcttcgtcg cctcgaacgt
      841 ggccaggtac agagccttta agagcgacgt gctgcccggg aacacgctcc gctggcggtt
      901 gagcttccgg taggtagagt tgaggctctc gatcgcgttg gtagtataga tcaccttcct
      961 gacctccatg gaaaacttga agatggggga taccacatcc cagttgtcat gccagcgttt
     1021 cattgagttg gggtactttg gcgtccactt ttccgtcacc ttctccagcg cctccagcgc
     1081 cttttcctcg tttgcggcat ggtagatggt tttcaggtcc gtggcaaatg ctttccggtc
     1141 cttgtcggag acgtatttca gcgtgttccg taccatatgt acgatgcagc gctgctgctc
     1201 cgtcttcggg aatgccgccg ttatggcttc cttaatgccc gtgagcccgt ccgagcagag
     1261 aatgaggata tccttcacac cgcggttctt caactcgttc agaacgcaga gccagtactt
     1321 ggagctttca ttctccccga tctgtatgct caggacttcc tttttacctt ccagcgtaag
     1381 gccgaggatg acataggcgg caagcttgcg gattaccccg tcctcccgca cggaataatg
     1441 gattgcgtcg atgtagacca cgggatagac ttcctccagc gggcggttct gccagtcttc
     1501 aatctgcggc agtatcttgt ccgtcacgtc cgagatgaac ccttccgaag cctcaaaacc
     1561 ataaatgtcc atcaaggtat ccgagatttg gcgggttgtc atgccttttg catacatgga
     1621 tatgatcttc tggtcaatgc cggaaatgtc cttctggcgc tttcttacca cctggggttc
     1681 aaacgtggac ttcctgtcct ggggcacctg gatgtccatg ctcccgaagc tgctgttgat
     1741 ccgcttgggc ttgtagccat tgcggtaatc atccgaatcc gaccgttctg acctcccata
     1801 gccgagatgc tcatccatct ccgcttccat catctccttc agggtgctcc ccagcaggtc
     1861 tttgagggct tcctggatat cctccgcagt ctggatgtca tactcttcca ggagctgatg
     1921 gacgatgttc cttttcccct ccgtcatgac tactttgtgt acgggctgtt tttctcttct
     1981 tgccataata ataggcctcc ttatgataat agattttatc atagaagacc taatgcttaa
     2041 tagctattta cagaaaaact ttcatactct caaagccgga ggcttcccct ggctcgcggg
     2101 cgttcgccct atgaaaaaac gcagccggcg atctgcttt
//
LOCUS       DBMDUH010000238         2100 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_17892_length_2100_cov_4.367184, whole genome shotgun sequence.
ACCESSION   DBMDUH010000238 DBMDUH010000000
VERSION     DBMDUH010000238.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17892_length_2100_cov_4.367184"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1272
                     /locus_tag="ACZPCG_13040"
     CDS             <1..1272
                     /locus_tag="ACZPCG_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HIR1286009.1"
                     /translation="AKEEKEIAAFAGFIGYYTFLVASSCMIGSGFMDFSALKISAILG
                     VETLDMGAVAGIISGLVTAKIHNKYHKVQFPVAISFYGGKRFVAIAVIMAMAAAGLIA
                     PLVWKPISAAIDGLGGLISATGLAGVFTYGFLERLLIPTGLHHVLNGLFRTTSLGGVY
                     EGVEGCLNIFLQFIDKVDINELAPFTVFLGQGKMPMMMFGLSGAALAIYRTSPEEKKG
                     KVKALMIAGVAASVVSGITEPLEFSFMFIAPALFVFHAVMGGLSFMLMAALHVIIGNT
                     GGGLIDYLIWGVFQPGSNWYWVLVVGPVFFVVYYRVFKWYLTKKKLSIDVADEEETDG
                     EQGVSMDEQQKAKAVKIIEGLGGFGNIGVVNNCLTRLRVDIKDMALVDEDILKKTGAM
                     GFVRSSDTHIQVIYGPKVERIASDVREVLKY"
     gene            complement(1309..2067)
                     /locus_tag="ACZPCG_13045"
     CDS             complement(1309..2067)
                     /locus_tag="ACZPCG_13045"
                     /inference="COORDINATES: protein motif:HMM:NF013539.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HIR1286010.1"
                     /translation="MIPLFEKAHRMQLTETEKSILDYFEKNLPLSVHTHLNDLSSALY
                     TSNATIVRFCQKLGLNGYNEFKYQLRHELKQMKEPVFSPNDLISHSLALFRDNMEAVD
                     ADKLKRIADYLTGDNPVYIYGSNLSSVPAKYLQVVLNTLDHPSILIEWHRLLAGLIHE
                     ITSDSVLFMISAHGDSQRYLPIFECAKKRGITTILLTSEKNSPLLSLSTIWMCSNDQN
                     EECHHVDINSRLGIITLIQLIIELAAQGTIKENS"
BASE COUNT          560 a          380 c          543 g          617 t
ORIGIN      
        1 gcaaaggaag aaaaggaaat agcggcattt gcaggcttta ttggctacta tacctttttg
       61 gtggcatcgt cctgtatgat aggatccggg ttcatggatt tcagcgcgtt gaagatttcc
      121 gcgattcttg gtgtggaaac actggacatg ggagcggtag caggtattat ttccggttta
      181 gttactgcaa agattcataa caaatatcat aaagtacagt ttcccgtagc aatttccttt
      241 tatggaggaa aacgattcgt agcgattgcc gtaattatgg caatggcagc agccggtctg
      301 atcgctcctc tggtatggaa gccgatttct gcagccattg acgggctggg cggccttatt
      361 tccgcaaccg gtctggcagg cgtatttacc tatggttttt tggaacgcct tctgattccg
      421 acaggacttc accatgtgct gaacggtctt ttcagaacca catctcttgg gggtgtttat
      481 gagggcgtag aaggctgtct gaatattttc ctgcagttta ttgataaggt tgatatcaat
      541 gaactggctc cgtttacggt attccttgga cagggtaaaa tgccgatgat gatgtttgga
      601 ctttccggcg cggctctcgc catttaccgt acttctccgg aagaaaagaa gggaaaggta
      661 aaagccctga tgatcgcagg cgtggcagcc agcgttgttt ccggtattac tgagcctctg
      721 gaattttcat ttatgtttat tgcacctgca ttgtttgtat ttcatgctgt tatgggcggt
      781 ctttcgttca tgctgatggc agcgcttcat gtgattatcg gaaatactgg aggcggattg
      841 atcgactacc tgatctgggg tgtgttccag cccggatcca actggtactg ggtacttgta
      901 gtaggacccg tattctttgt tgtctactat cgggtgttta aatggtatct gacaaaaaag
      961 aagctttcaa tcgatgtggc agatgaagag gaaacagacg gggagcaggg tgtttccatg
     1021 gatgagcagc agaaagcaaa agcggtaaag attattgaag gacttggcgg ctttggcaac
     1081 atcggtgtgg taaataactg tctgacaaga cttcgtgtgg atatcaagga tatggctctt
     1141 gttgatgagg atattctcaa gaagacaggg gcaatgggat ttgtacgatc gagtgatacg
     1201 catattcagg ttatttacgg cccgaaggta gagcggatcg catccgatgt gagggaagta
     1261 ttaaaatatt gatcaataaa aagagaactt ctgatttgaa aacttggatc aggagttctc
     1321 ttttattgta ccctgtgctg ccagttcaat aatcagctgg atcagggtga tgatgccgag
     1381 acgggagttg atatctacat gatgacattc ttcattctga tcattgctgc acatccagat
     1441 ggtgcttaaa gaaagcagag gactgttttt ttcactggta aggagaatag tggtaattcc
     1501 tctttttttg gcacattcaa aaatcggaag atagcgctga gaatctccgt gggcggaaat
     1561 cataaataaa acagaatccg aggtgatctc atggatcagt ccagccagaa ggcggtgcca
     1621 ttcgataaga atcgatgggt gatccaatgt atttaaaacg acctgaagat attttgccgg
     1681 gacagagctt aaattggaac catagatata tacgggattg tcgccggtaa gatagtcggc
     1741 aatgcgtttt aatttgtctg catcgacagc ttccatatta tctctaaaca gagccaggga
     1801 atgggaaatc agatcattag gagaaaaaac gggttccttc atctgtttta actcatgacg
     1861 gagctggtat ttaaattcat tatatccatt taagcccagt ttttgacaaa aacgaacgat
     1921 ggtagcgttt gaggtataaa gagcagaact caggtcattt aaatgagtgt ggacagagag
     1981 gggaaggttt ttttcaaaat aatccagtat gctcttttct gtttccgtaa gctgcatacg
     2041 gtgcgctttt tcaaataatg gaatcatggg tatctcctaa acgtttgtat tccagatagc
//
LOCUS       DBMDUH010000239         2086 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_18057_length_2086_cov_6.655416, whole genome shotgun sequence.
ACCESSION   DBMDUH010000239 DBMDUH010000000
VERSION     DBMDUH010000239.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18057_length_2086_cov_6.655416"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="ACZPCG_13050"
     CDS             complement(<1..602)
                     /locus_tag="ACZPCG_13050"
                     /inference="COORDINATES: protein motif:HMM:NF023650.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan binding domain-containing protein"
                     /protein_id="HIR1286011.1"
                     /translation="MRKNKTALFLAAVSLSAFFAVSSPGAQEDASRFVDGTRINSVGV
                     GGLTPEEARERIQGFYAGEYELSVIKKDGSREVIRGEAIDYQVALTDDLDAILKAQNE
                     GGRQSGPSVDNSHQAALVPSYSQEKLDQAIEALSVLNSSAVTVTKDASISPYEEGKPF
                     SIVPAVQGNDVDREKTILAVNEAVKAGRNELDLEAEGCYRT"
     gene            complement(741..1484)
                     /locus_tag="ACZPCG_13055"
     CDS             complement(741..1484)
                     /locus_tag="ACZPCG_13055"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003524494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR1286012.1"
                     /translation="MKKWMTVCLAAVTAAVFMAGCGSTSDAKETAKETAAASSEAETT
                     KAETAAESEKETAAEDGTAQADEKSTAGHKESLDLQAAAGKHHIEINVKDYGTIFVEL
                     NGDSAPITVANFMDLAEQGFYDGLTFHRIISGFMIQGGDPMGNGMGGSEREIKGEFSS
                     NGVENDLSHTRGAISMARSQMKDSASSQFFIVHEDSTFLDGEYACFGYVTDGMEVVDA
                     ICKDTQVEDNNGTVAKDNQPVIDSIKVID"
     gene            complement(1631..>2086)
                     /gene="thiD"
                     /locus_tag="ACZPCG_13060"
     CDS             complement(1631..>2086)
                     /gene="thiD"
                     /locus_tag="ACZPCG_13060"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0042724 - thiamine-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HIR1286013.1"
                     /translation="RLIGEDAVEMLKERLLPLAALATPNIPEAEVLSGCEIRSEEDME
                     TAARIISQTYGCSVLLKGGHRINDANDYLYRMGHGIWLYGKRINNPNTHGTGCTLSSA
                     IASNLAKGMELEAAVAQAKRYLSAALEAQLDLGRGSGPMDHGFAVRGEY"
BASE COUNT          469 a          595 c          412 g          610 t
ORIGIN      
        1 gttcggtagc agccttctgc ctccagatcc aattcatttc ttcctgcctt tacagcctca
       61 tttactgcca aaatcgtttt ttcccgatcc acatcatttc cctgtactgc gggaacaatg
      121 gaaaatggct ttccttcctc atagggtgaa atcgaagcat cctttgtaac tgtaacagcg
      181 ctgctgttta atacggaaag tgcttcaatg gcctgatcca gcttttcctg gctgtaggag
      241 ggaacaagcg ctgcctgatg tgaattgtct accgaggggc cggattgcct ccccccttcg
      301 ttctgagcct tcaggatagc atccagatca tctgtcaacg caacctgata atcaattgct
      361 tctccccgga tcacctccct gctcccgtcc ttttttatga cagaaagctc gtattcgccg
      421 gcataaaatc cctggatccg ttctctggcc tcctccggtg tgagtcctcc cacacctacg
      481 ctgttgatcc tggtaccgtc cacaaaccgc gaagcgtctt cctgagctcc cggggaagaa
      541 acggcaaaaa aagcagacaa agaaacggct gccagaaaaa gagcagtttt atttttacgc
      601 ataaaaacat cctttctgat ataaaatctc atattttatt ctagcatact tttccttctg
      661 tgcatcttac aattgcctac aatttttatt aaaaaatgta aaagagaccc cccttcccga
      721 aagatctcct ttgaacttga tcagtcaatg accttaatgc tgtcgatcac cggctgatta
      781 tcctttgcaa ctgtaccatt attatcctct acctgagtat ccttacagat tgcgtccaca
      841 acctccatac catcggttac atagccaaag caggcgtatt caccgtccag gaacgtactg
      901 tcctcatgta caataaaaaa ctgggagctt gcactgtcct tcatctggga acgtgccatg
      961 gaaatggcgc ctcttgtgtg agataagtca ttctctacac cattggagga aaattcgcct
     1021 ttgatttccc gttctgagcc tcccatacca tttcccatgg gatctcctcc ctgaatcata
     1081 aatccgctga tgatccgatg gaaggtaagt ccgtcataaa atccctgttc tgccagatcc
     1141 atgaaatttg ccactgtgat cggggcgctg tctccgttca gctctacgaa aatggtgccg
     1201 taatccttta cattgatctc tatatggtgc tttccggctg cagcctgcag atccagactc
     1261 tccttatgac ctgctgtact cttttcatct gcctgggctg ttccgtcttc cgctgctgtc
     1321 tctttctcag actctgctgc tgtttctgcc tttgtagttt ctgcctctga agaagccgct
     1381 gctgtctcct ttgctgtttc ctttgcatct gatgtactgc cgcatccggc cataaatact
     1441 gctgctgtca ctgcggccag gcagactgtc atccattttt tcatcctgta ttccttcctt
     1501 tcccgtccgt tcatctgccc cttttttctg ataagaggca gccagatcat tgtaccattc
     1561 cggatatcca caagtaaagg actttataaa aatatcacag tttagtcaca atttgcctaa
     1621 agcactgtgc ttaatattct cccctgacag caaagccgtg atccatgggg ccgcttcccc
     1681 ttcccagatc aagctgtgct tccagggcag cagaaagata ccgcttcgcc tgagcgactg
     1741 cagcctccag ttccattccc tttgccaggt tggaagcaat ggcgctggac agggtacagc
     1801 cggtaccatg ggtattggga ttgttgatcc tttttccgta aagccagatt ccatggccca
     1861 ttctgtataa ataatcatta gcatcattga tccggtgccc tccctttaag agcacactgc
     1921 agccatatgt ctgtgaaatg atcctggccg ctgtttccat gtcctcttca gaccggattt
     1981 cacagccgga aagcacctct gcctccggta tattgggcgt cgccaaagct gccagaggca
     2041 gcagccgctc tttcagcatc tccaccgcat cttctcctat gagtct
//
LOCUS       DBMDUH010000240         2071 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_18238_length_2071_cov_7.180203, whole genome shotgun sequence.
ACCESSION   DBMDUH010000240 DBMDUH010000000
VERSION     DBMDUH010000240.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18238_length_2071_cov_7.180203"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="ACZPCG_13065"
     CDS             <1..517
                     /locus_tag="ACZPCG_13065"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR1286014.1"
                     /translation="SLALEIKTDGQVIIRAPEGISEGVLMEFVRTRQEWILKKWFDSE
                     TKRRNLAQVFPHDYEEDPKLEAAYRRKARERIGQRTAYFAALMGVTYGRISIRSAKTR
                     WGSCSSEGNLNFHWKLVLMPPEVLDYVVVHELAHRKEMNHSRKFWAEVKNVLPDYEKR
                     RKWLKEFGGYV"
     gene            complement(616..975)
                     /gene="trxA"
                     /locus_tag="ACZPCG_13070"
     CDS             complement(616..975)
                     /gene="trxA"
                     /locus_tag="ACZPCG_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HIR1286015.1"
                     /translation="MQQKTNSHLQEVIYMALSHLTSADFDQAVNAGDDVVVVDFFATW
                     CGPCKMLAPAVERMAEVHPEVHFYKVDIDEEMDLASRFGVMSVPTLLYFKRGEIVNKT
                     VGLISPADLENAIAKSK"
     gene            complement(1036..1620)
                     /locus_tag="ACZPCG_13075"
     CDS             complement(1036..1620)
                     /locus_tag="ACZPCG_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HIR1286016.1"
                     /translation="MIYLQLFLSFLQIGAFSFGGGYAAMPLIQNQVVQLHPWLSQSEF
                     TDLITISQMTPGPIAVNSATFVGTRIAGVPGALAATIGCVLPSCILVTILAKIYLKYR
                     SLSLLQGILKSLRPAVIAMIAAAGVSILVTAFWGNDISSLHLDAILSSTNIRAVGIFL
                     LSLILLAKFKMDPIHVMLLSGGAEVVMQLIMKLL"
     gene            complement(1617..>2071)
                     /locus_tag="ACZPCG_13080"
     CDS             complement(1617..>2071)
                     /locus_tag="ACZPCG_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HIR1286017.1"
                     /translation="HWIDEEEMLDRVAIAQSCPGAIAVNGAIVVGYKLAGLPGVMTSV
                     LATVIPPFSILSLISLCYAAFRSNQYVGWMLNGMQAGVGAVIMEVVWEMSSGIIKEKQ
                     TVPLLVMAAAFIANYFFGVSAIIIILLCIGLGIVTTLSREKQKKGGSL"
BASE COUNT          568 a          441 c          580 g          482 t
ORIGIN      
        1 cagtctggca ctggagataa agacggatgg tcaggtgatc atccgtgcgc cggagggcat
       61 ttcggaagga gtgctgatgg agtttgtacg aacccggcag gaatggattc tgaagaaatg
      121 gttcgattcg gaaacaaaga gacgaaatct ggctcaggta tttccacatg actatgagga
      181 agatccaaag cttgaggcgg cttaccgcag gaaggcacgg gagcggattg ggcagaggac
      241 tgcgtatttt gcggctttga tgggagtaac ctatgggcgg atttccatcc gcagcgccaa
      301 aacacgctgg ggaagctgca gcagtgaggg gaatttaaat ttccactgga agctggtgct
      361 gatgccgccg gaggttctgg attatgtggt ggtccatgag ctggctcacc gaaaggagat
      421 gaaccattcc cggaagtttt gggcagaagt aaagaatgtg cttcccgatt atgagaagag
      481 aagaaagtgg ctgaaagaat ttggaggata cgtgtaggta ccctgatttc cggttgcagg
      541 acaataaaag aggattgaat gcttcgtttg acagatatcg cagcgaaggg ttcaatcctc
      601 ttatgtgttt atggtttatt tggatttggc aatggcattt tccagatcag caggagaaat
      661 gaggcctacg gttttattta cgatctctcc tctcttgaag tagagaaggg tgggaacgct
      721 catgactccg aaacgggatg ccagatccat ttcttcatca atgtctacct tgtagaaatg
      781 tacctccgga tgaacctctg ccatgcgttc tactgcggga gccagcattt tgcagggacc
      841 gcaccaggtt gcaaagaaat caacgactac tacgtcgtca ccggcattta ctgcctggtc
      901 gaaatcagcg gatgtgagat gtgaaagtgc catatatatt acctcctgta aatgtgaatt
      961 cgttttctgt tgtatatagg ataccacagc cgcccctctt tttatgtgat aaaagtcacg
     1021 aaacagaaaa aatatttaca gaagcttcat aatgagctgc atgacaactt ccgcccctcc
     1081 ggaaagaagc atcacatgga tgggatccat tttgaatttt gcaagaagaa tcagggaaag
     1141 cagaaagatt cccacagctc ttatatttgt actggagaga atggcatcca gatggaggga
     1201 ggaaatgtca tttccccaga atgcggtaac cagaatgctg actccggcag ctgcgatcat
     1261 ggcgattacg gcaggacgga gggattttaa aatgccttga agaaggctta aacttcggta
     1321 ttttaagtaa atttttgcca gaatcgttac aagaatgcag gaagggagca cacagccgat
     1381 ggtagctgcc agcgctccgg gaactccggc aatgcgggtg cctacaaagg ttgcggaatt
     1441 gactgcgatc gggccggggg tcatctggga aatggtgatc agatccgtaa attcggactg
     1501 ggaaagccag ggatgaagct gtactacctg attctggatc agaggcattg ccgcatagcc
     1561 gccgccgaag ctgaaggcgc caatctgcag aaagcttaaa aagagctgta aatagatcat
     1621 aaggaaccgc ctttcttctg tttttcacgg cttaaggtag tgacgatacc aaggccgatg
     1681 cacagcagga taatgataat ggcgctgacg ccgaagaagt agttggcaat aaaggccgcg
     1741 gccatgacaa gaaggggaac tgtctgcttt tcttttataa tgccagagga catttcccag
     1801 actacctcca tgattacggc tcctactccg gcctgcattc cgtttaacat ccagcccaca
     1861 tactgattgg agcggaaggc ggcatagcag agtgaaatca gagaaaggat ggaaaaggga
     1921 gggatgaccg ttgcaagaac ggaggtcatg actccgggaa ggcctgccag cttgtagcct
     1981 accacgatag ccccgttgac tgcgatggca ccggggcagg actgggcaat ggctacccga
     2041 tccagcatct cttcctcatc gatccagtgg t
//
LOCUS       DBMDUH010000241         2064 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_18319_length_2064_cov_4.431482, whole genome shotgun sequence.
ACCESSION   DBMDUH010000241 DBMDUH010000000
VERSION     DBMDUH010000241.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18319_length_2064_cov_4.431482"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..135
                     /locus_tag="ACZPCG_13085"
     CDS             <1..135
                     /locus_tag="ACZPCG_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003498656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGGtGRT protein"
                     /protein_id="HIR1286018.1"
                     /translation="FAGSSSVPAHVEMMGLIGMGNNPMVGATVAVAVSIEEAAKEGKF
                     "
     gene            372..752
                     /locus_tag="ACZPCG_13090"
     CDS             372..752
                     /locus_tag="ACZPCG_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HIR1286019.1"
                     /translation="MSEENRGTHKLYEKDRIGEVQIADEVVAIIAGLAATEVEGVDSM
                     AGNITNELVGKLGMKNLSKGVKVDVTENHVSVDLSLNIRYGFNIPSVSEQVQEKVSAA
                     IENMTGLTVLDVNIRIAGVNMEEN"
     gene            922..1440
                     /gene="nusB"
                     /locus_tag="ACZPCG_13095"
     CDS             922..1440
                     /gene="nusB"
                     /locus_tag="ACZPCG_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007719970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HIR1286020.1"
                     /translation="MTRRELREHCFKMLFSADFYKTTEEAKEQMWQYFQSPQEYETND
                     EGSLTILHKVELTDKDMEYLQTKTGNIVDKIQEIDSRIDEVAAGWKTRRMGKVELTIL
                     RLALYEMDYDDTVPAKVAVNEAVELAKKFGGSDSPAFVNGVLAKFIRKEETTTETEPA
                     NEKTAQAQETEA"
     gene            1462..>2064
                     /gene="xseA"
                     /locus_tag="ACZPCG_13100"
     CDS             1462..>2064
                     /gene="xseA"
                     /locus_tag="ACZPCG_13100"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HIR1286021.1"
                     /translation="MASVFTVSQVTSYIKNMFTQDFALNRISIRGEVSNCKYHTSGHI
                     YFTLKDGGAQIAAVMFAGQRKGLSFTLQEGQEVVVKGTVDVYERDGRYQLYAREITRE
                     GIGDLFARYEKLRTELEEMGMFDSCYKRPIPRYARTIGIVTARTGAAIRDIMNIAARR
                     NPYVQLVLYPALVQGEQAKYSIAKGIETLDRMGLDLLIVGR"
BASE COUNT          613 a          376 c          567 g          508 t
ORIGIN      
        1 ttcgcaggtt cttcctccgt tccggctcac gttgagatga tgggtctgat cggtatgggt
       61 aataacccaa tggttggcgc tactgtagct gtagctgttt cgattgagga agctgctaag
      121 gaaggcaagt tctaataaaa aaccttggat ttacaaggaa aaatgaacct ttaaacaaag
      181 aggggaatcg tcttttgacg gttcctcttt tgtggctttt gggatataga tgcggttttg
      241 tctaccattt ggacttctta cgggaggaat tgtcgcctat tcaaagagaa atccccctat
      301 acagatttcc cacgatttgt tataataaag agtaataaaa atcaagcttc aggaacagga
      361 ggtaaagagg attgtctgag gaaaatcgcg gtacccataa attatatgaa aaagacagga
      421 tcggagaggt tcagatcgca gatgaggtgg tagccattat tgcagggctg gcagctacag
      481 aggttgaggg agtagactct atggcaggca atattaccaa tgagctggta ggaaagcttg
      541 gcatgaagaa tcttagcaag ggagtaaagg tggatgtgac agagaatcat gtatctgtgg
      601 atctttcctt aaatatccgt tatggcttca atatcccttc tgtcagcgag caggtgcagg
      661 aaaaggtcag tgctgccatc gaaaatatga cggggcttac agtgctggat gtgaatatcc
      721 ggattgcagg cgtgaatatg gaagaaaact gacagaaagt ttaatgcgtt tgggtgctca
      781 ggtccgaata agggtctgca gcaccttttg atatttttta ttgtgtttat ccggcgcctt
      841 tggtataatc acagttagcg gatcagaata gagaaaggaa aatgtattcg agagcccagg
      901 cttttcgtac aagcagaaga tatgaccaga agagaattaa gagaacactg ttttaaaatg
      961 ctgttctccg cggattttta taaaaccaca gaggaagcaa aggagcagat gtggcagtat
     1021 ttccagtctc ctcaggaata tgagacaaat gatgagggaa gccttacgat ccttcataaa
     1081 gtagagctta cagataagga tatggaatat ctgcagacaa aaacaggaaa tatagtagat
     1141 aagatccagg aaatcgacag ccggatcgat gaggtggctg caggctggaa gacaagacgt
     1201 atgggaaagg tggagcttac tattcttcgt ctggcccttt acgagatgga ctatgatgat
     1261 acggttcctg caaaggtagc ggtgaacgag gcggtggaac ttgctaagaa gtttggagga
     1321 agtgactctc cggcttttgt aaatggtgta cttgcaaagt ttatccgcaa agaagagaca
     1381 acgacagaga ctgaaccggc gaatgagaag actgcgcagg ctcaggaaac agaagcgtag
     1441 gaaagaaagc agagggagca gatggcaagt gtatttaccg tatcccaggt gacctcatat
     1501 atcaaaaata tgtttaccca ggattttgcc ctgaaccgga tctccatccg gggcgaggtt
     1561 tccaactgta aataccatac ctccggtcat atatatttta cgttaaagga cggcggcgct
     1621 cagattgccg ccgtcatgtt tgcaggccag agaaagggac tttcttttac actgcaggag
     1681 ggccaggagg tagtggtaaa gggaacggta gacgtatatg aacgggacgg ccgctatcag
     1741 ctctatgcca gagagattac aagagaagga atcggtgatc tttttgccag atatgaaaag
     1801 ctgcgcacgg aattagaaga aatggggatg tttgattcct gttataagcg tcccattccc
     1861 agatatgcca gaacgattgg aatcgtgaca gccagaaccg gagctgccat ccgggatatt
     1921 atgaatattg cggccagacg caatccctat gtacagctgg tgctgtatcc tgctctggtg
     1981 cagggagagc aggccaaata cagtattgca aagggaatcg agactctgga ccggatggga
     2041 ctggatcttc tgatcgtagg ccgg
//
LOCUS       DBMDUH010000242         1962 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_19593_length_1962_cov_4.665234, whole genome shotgun sequence.
ACCESSION   DBMDUH010000242 DBMDUH010000000
VERSION     DBMDUH010000242.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19593_length_1962_cov_4.665234"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1314
                     /locus_tag="ACZPCG_13105"
     CDS             <1..1314
                     /locus_tag="ACZPCG_13105"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012200748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HIR1286022.1"
                     /translation="RRFGARGDGKHDDTVAIQAAVSSCPEHGRVYIPKGVYKVSSIFL
                     KSHMTLDIGEGAVISAYTEREKFPILPGLIESYDETDEYNLGTWEGNPLDMFGSILTG
                     IHVSDVVITGEGTLDGNASYENWWKGEGREKIGGAFRPRMIFLNHCENVTVQGLTVQN
                     SPAWNLHPYFSNHTRWIDLKVLNPKQSPNTDGMDPESVDGLEVVGVYFSLGDDCIAMK
                     SGKFYMGHKYKVSSRNVDIRQCYMRHGHGAVTLGSEIAAGVRHLSCKKCIFEDTDRGL
                     RVKTRRGRGEDSVVEDILFEDIKMDGVLTPFVVNSYYWCCDPDGHSTYVSTKEPLPVD
                     ERTPRIKELAFRNIEAHNCHVAGTFIYGLPEAKIEKVTMENIEIDFAENPTPEYPAMM
                     AEVEPCTNRGVFINNVKELTLKNVKVHGTAGEPFEIENVDCVEKE"
     gene            1318..>1962
                     /locus_tag="ACZPCG_13110"
     CDS             1318..>1962
                     /locus_tag="ACZPCG_13110"
                     /inference="COORDINATES: protein motif:HMM:NF013429.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HIR1286023.1"
                     /translation="MELAITSTSMKLRDQAPVVFRGEDNWCFEQIRKIGYDGVELHIH
                     DSSQLDRKQLKEELDQFGLSLTSIGTGSAYGKDRVFLSSDDAAVRQAAIERIKGHILT
                     ASDYPHAVVIIGLIKGKVSDCESRESYFSNLISALQECAEEAGKYQVYLGLEVINRYE
                     SDVINTVEEGLELLELVGSPWLQLHLDTYHMNIEESDIKKSIQSAKGKICHVHVA"
BASE COUNT          584 a          343 c          528 g          507 t
ORIGIN      
        1 cgccgtttcg gagcgagagg cgatggaaag catgatgata cagttgctat tcaggcggcg
       61 gtgtcatcct gtccggagca cggaagagtg tatattccca aaggagttta taaagtatcc
      121 agtatctttc tgaagagcca tatgacactg gatattgggg aaggagcagt gatttctgct
      181 tatacggaac gggagaaatt tcccatttta ccgggactga ttgagagcta tgatgagacg
      241 gatgaatata atctgggaac atgggaggga aatcctctgg atatgttcgg atctattctt
      301 acgggtattc atgtttcaga tgtggtaatt accggagaag gaactctgga tggaaatgcc
      361 agctatgaaa actggtggaa gggggagggc cgtgaaaaaa tcggaggagc cttccgccca
      421 agaatgattt ttttgaatca ctgtgaaaat gttactgttc agggactgac tgttcagaac
      481 agtccggcct ggaaccttca cccatatttt tccaatcata cgcggtggat tgatttgaag
      541 gtactcaatc ccaagcagtc cccgaatacg gatggaatgg accctgaatc agtagacggt
      601 ctggaagtag taggcgtata tttctcctta ggtgacgact gtattgccat gaagtccggt
      661 aagttctata tgggacataa atataaggtt tcatcaagaa atgttgatat tcgacagtgc
      721 tatatgagac atggacacgg agcggtaact ctgggcagcg aaattgctgc cggggtgaga
      781 catttgtcct gtaagaagtg tattttcgaa gatacggacc gcggattgcg cgtaaagacc
      841 agaagaggcc gtggagaaga ttctgtagtt gaggatattc tgtttgaaga tattaagatg
      901 gatggagttt taacaccctt tgtagtaaat tcctactact ggtgctgtga tcctgacgga
      961 cattctacct atgtaagcac aaaggaaccg cttccggttg atgaacggac gcccagaata
     1021 aaagaactgg ccttccgcaa cattgaagct cataattgtc atgtggcagg aacctttata
     1081 tatggacttc cggaagccaa aatagaaaaa gtgacaatgg aaaacataga gattgatttt
     1141 gcggaaaatc caacgccgga gtatcccgca atgatggctg aggttgaacc ctgtacaaac
     1201 cgaggcgtgt ttatcaataa tgtaaaggaa ctcaccttaa agaatgtaaa ggttcacggc
     1261 acagcaggag aaccctttga aattgaaaat gtagattgtg tggaaaagga gtaaacgatg
     1321 gagttagcga taacaagtac cagcatgaag ctgagagatc aggccccggt ggtttttcgg
     1381 ggtgaagata actggtgctt tgaacagatc cgtaaaatag ggtatgatgg cgtagagctt
     1441 catatccatg attcctctca gcttgacagg aagcagttaa aggaggaatt ggatcagttt
     1501 ggcctgagtt tgacttcgat tggaacagga tctgcctatg gaaaagacag ggtgttttta
     1561 tccagcgacg atgcggctgt aagacaggct gcaattgaac gtattaaggg acatatcctt
     1621 acggcatctg attatcctca tgctgtggtg attatcgggc tgattaaggg aaaggtatca
     1681 gactgcgaaa gcagagaaag ctatttttcc aatttaattt cagccttgca ggaatgtgca
     1741 gaagaagcgg gaaaatatca ggtatacctt gggcttgagg ttatcaacag gtatgaaagc
     1801 gatgtgatta ataccgtaga ggaagggctt gagcttctgg aactggttgg gtctccatgg
     1861 ctgcagctgc atctggatac ataccatatg aatattgaag agagtgatat taaaaaatca
     1921 attcaatcag ctaaggggaa aatctgtcat gtacacgttg ca
//
LOCUS       DBMDUH010000243         1830 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_21457_length_1830_cov_5.209370, whole genome shotgun sequence.
ACCESSION   DBMDUH010000243 DBMDUH010000000
VERSION     DBMDUH010000243.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21457_length_1830_cov_5.209370"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            287..712
                     /locus_tag="ACZPCG_13115"
     CDS             287..712
                     /locus_tag="ACZPCG_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007712399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1286024.1"
                     /translation="MIVLDYRDRRPLYEQVTERLEELMFQEILTENTKLPSVRSLATE
                     FSINPNTIQRAYAELEQRGYIYSVKGKGSFVAEGSRLRSGRKKAWEASFDRAVKDGIQ
                     IGISPGEMTERLERMLNLEKNKEEGGDDHDSGSEADETV"
     gene            678..1577
                     /locus_tag="ACZPCG_13120"
     CDS             678..1577
                     /locus_tag="ACZPCG_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009298092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1286025.1"
                     /translation="MIQAVKLTKRFEDITAVDHIDAAIREGSVFGLIGTNGAGKSTFL
                     RLASGILKPDEGHITIDGQEVFENPAAKRMVFYISDEAYFFSNASPLDMMAYYSRVYE
                     RFDKERFRRLLKSFGLDEKRRIHTFSKGMKKQVSVICGICANTKYLFCDETFDGLDPV
                     MRQAVKSLFAADMEDRNLTPIIASHNLRELEDICDHVGLLHKGGILLSKDLDDMKLNI
                     HKIQCVLQPGMEPEDLVSLEVLSTECRGRLLTLTVRGTREEIEAKLGACDPVFFECIP
                     LTLEEIFISETEVAGYDVKKLVF"
     gene            1552..>1830
                     /locus_tag="ACZPCG_13125"
     CDS             1552..>1830
                     /locus_tag="ACZPCG_13125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1286026.1"
                     /translation="MTSKNLFSEKKVCGRIWGLMKEDIKRRIWVLALMFLAFFFALPV
                     KLALVMENARRMEFRSYNDYEPIEKLLPEHFTAKQYEMMTAFFKREVVL"
BASE COUNT          510 a          347 c          495 g          478 t
ORIGIN      
        1 tgcagcattc cactgctgta tccctttttc gtctatccac tccatccgct taaggatcat
       61 tgaaaacatc tggggaaccc ctgattttgt gatttctctg aattgatatc tgctttgcat
      121 ggggagacct cctgaatctg atgctgtttg aatgaattgt accacaaact gtcaagaatc
      181 gtaagaaaat tgttattgta tttcagactg gttcatggta ttgttaaatc aggataactg
      241 tattaagaga attagtacag ttaaaacaga gaaaggagag attccaatga tcgtactgga
      301 ttacagggat cggaggcctc tgtatgaaca ggtcacagaa cggttggagg agctgatgtt
      361 tcaggaaatc ctgacggaaa atacaaagct tccctcagtc cgcagccttg cgacggagtt
      421 ttccatcaat cccaatacca tccagagagc gtatgcagaa ctggagcaaa gaggctatat
      481 ttattctgtt aagggaaagg gcagcttcgt ggcggaaggg agccggttaa ggagcggaag
      541 aaaaaaagcc tgggaagcga gctttgaccg tgcggtgaag gatggtatac agatcggcat
      601 ttctccggga gaaatgacag agcgactgga acggatgctt aatcttgaga aaaataagga
      661 agaaggggga gacgaccatg attcaggcag tgaagctgac gaaacggttt gaggatatta
      721 cggcagttga tcatatagac gctgccatca gggaaggaag cgtgtttgga ctgatcggta
      781 ccaatggtgc aggaaaatct acttttttac gtcttgcaag cggcatttta aagccggatg
      841 agggacacat taccattgac ggccaggagg tatttgaaaa tccggcagct aaaagaatgg
      901 ttttttatat atccgatgag gcatattttt tcagcaatgc atcgcccctt gacatgatgg
      961 cttattattc ccgtgtgtac gagcgttttg acaaggagag gtttcgccgc ctgttaaaaa
     1021 gttttggact ggatgaaaag cgcaggatcc acactttttc caagggaatg aaaaaacagg
     1081 tgtccgtgat ctgcgggatt tgtgctaata caaagtatct gttctgcgac gagacatttg
     1141 acggactgga tccggtcatg cgccaggcag taaagagcct gtttgccgcc gatatggaag
     1201 accggaatct gacgcctatc atcgcatccc acaatctcag ggagctggaa gatatctgcg
     1261 accatgtagg cctgcttcat aagggaggaa tcctcctttc aaaggatctg gatgatatga
     1321 agctgaatat tcacaagatc cagtgtgtac tgcagcccgg aatggagccg gaggatctgg
     1381 taagtcttga agttctgtcc acagagtgca gaggaaggct tcttacgctg accgtccggg
     1441 gaacgcggga agagatcgag gcaaagcttg gagcttgtga tcctgtattt tttgaatgta
     1501 ttcctctgac tctggaggaa atttttatca gtgaaacgga ggtggcagga tatgacgtca
     1561 aaaaacttgt tttctgaaaa aaaggtctgt ggcagaatat ggggactgat gaaagaggat
     1621 atcaaacggc gtatctgggt gctggcgctg atgtttctgg cttttttctt tgcccttccg
     1681 gtaaagctgg cattagttat ggaaaatgcc cggcgaatgg agtttcgctc ctataatgac
     1741 tatgagccga ttgaaaagct gcttccggag catttcactg ccaagcagta tgagatgatg
     1801 actgcatttt tcaagaggga agttgtactg
//
LOCUS       DBMDUH010000244         1760 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_22576_length_1760_cov_5.611814, whole genome shotgun sequence.
ACCESSION   DBMDUH010000244 DBMDUH010000000
VERSION     DBMDUH010000244.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22576_length_1760_cov_5.611814"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="ACZPCG_13130"
     CDS             complement(<1..881)
                     /locus_tag="ACZPCG_13130"
                     /inference="COORDINATES: protein motif:HMM:NF015557.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIR1286027.1"
                     /translation="MKLRVISFIKRETILTVSFFLALLSCFLVKPDREYLRYFTSNMG
                     TMIILFCLMAVVAGLGSLGVFRYLGERLLERIRSQGGIMLLLVFLCFFGSMLITNDVA
                     LITFVPFGIMILEMAAMREKICCTVTLMTIGANLGSMFTPMGNPQNLYLYGLSGMKLL
                     EFLFLMLPLTAAAALLLALSVVFYSKKGKIKLQMEEKTERPDLVRTAFYVFLFLLCLQ
                     TVNGTLPAEILLILILAAVAAGDKRLFAKVDYSLLLTFLCFFVFIGNINRFGPFRELA
                     VRVISGHELAASVIMSQV"
     gene            969..1244
                     /locus_tag="ACZPCG_13135"
     CDS             969..1244
                     /locus_tag="ACZPCG_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HIR1286028.1"
                     /translation="MTFTYPAVFTPHKEDKGFHAYFPDLECCEADGPDLEDAVENARD
                     AARDWITVELEDTCDIPPCSHADDIELKEGEFVKFIMVTIKLLPDND"
     gene            complement(1323..>1760)
                     /locus_tag="ACZPCG_13140"
     CDS             complement(1323..>1760)
                     /locus_tag="ACZPCG_13140"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009757242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HIR1286029.1"
                     /translation="DMKVSHETLLRALTLSNLTAIHQKTAIGRLSAYCGAVSAGAGAG
                     AGIAYLHGGDYKEIVHTVVNALAIVSGIVCDGAKASCAAKIASAVDAGILGYQMYIRG
                     QQFYGGDGIVTKGVEETLKNVGRLGKEGMKATNEEIIRIMIGE"
BASE COUNT          496 a          467 c          367 g          430 t
ORIGIN      
        1 acctggctca ttatgacaga ggctgccagc tcatgacctg agatcactct taccgccagt
       61 tcgcgaaaag ggccaaaccg gtttatattt ccaataaaca caaagaagca taaaaaggtc
      121 agcaaaaggg aatagtccac ctttgcaaac aaccgtttat cgcctgccgc cacagctgcc
      181 agtataagga tcagaagtat ctctgccgga agtgttccgt ttaccgtctg aaggcagagc
      241 aggaataaaa acacataaaa agccgtccgc accagatccg gcctttccgt cttttcttcc
      301 atctgaagct taattttccc tttctttgaa taaaatacca cactgagagc caaaagaaga
      361 gccgcagccg ccgtaagcgg aagcatcagg aaaagaaatt ccagcagctt catgccggat
      421 aagccataca ggtaaagatt ctgcggattt cccatagggg taaacatact gcccagattg
      481 gctccgattg tcatcagcgt aaccgtacag cagatttttt ctctcatggc cgccatttca
      541 aggatcatga tcccaaaggg aacaaaggtg atcagcgcca catcattggt aataagcata
      601 ctgccaaaaa aacacaaaaa caccagaaga agcatgatcc ctccctgaga ccggatccgt
      661 tccaaaagcc gctctcccag ataccggaac acgcccaggc ttccaagacc cgctaccact
      721 gccatcaggc agaacagaat gatcatcgtt cccatattag aagtaaaata cctcagatac
      781 tccctgtccg gctttaccag aaaacaggat aagagggcaa ggaaaaagga aacggtcagg
      841 atcgtttccc ttttgataaa tgatattaca cgcagcttca taattttatt tttcattcct
      901 ttctgttctg tacaaataac cagaaccgcg ctataatacg ttttatcaca aaccggaggt
      961 atttttcaat gacatttaca tatcctgctg tttttacccc tcacaaagag gataaaggct
     1021 ttcacgcata tttcccagat ctggaatgct gcgaagccga cggacccgat ctggaggacg
     1081 cagtagaaaa tgcaagagat gcggccagag actggatcac cgtagagctg gaggacacct
     1141 gcgacatccc gccctgcagc catgcagatg atattgaatt aaaagaagga gaatttgtca
     1201 aatttatcat ggtaacgatc aagcttctgc cggataatga ctgattgttt ccgcaaaaaa
     1261 gctctgcctt acgctgtgat ctgctttcac agcgtatgca gagccttttt tatttcctga
     1321 ttttattctc cgatcatgat ccggatgatt tcttcattcg tagccttcat tccttctttt
     1381 cccaggcggc ccacattttt aagcgtttcc tctactccct ttgtgacaat gccgtcaccg
     1441 ccgtaaaact gctggccgcg gatatacatc tgatatccca aaatgcctgc atctaccgca
     1501 gaagcaatct ttgccgcgca ggaagccttc gctccgtcgc agacaattcc ggacacaatg
     1561 gcaagggcgt ttaccaccgt atgtacgatt tctttgtaat ctccgccatg gagataagcg
     1621 attcctgccc ctgctcccgc tcccgcactg acagcaccac agtaggcaga aagccttccg
     1681 atggctgttt tctggtggat ggccgtcagg ttggaaaggg tcagcgcccg cagcagggtt
     1741 tcatggctta ccttcatatc
//
LOCUS       DBMDUH010000245         1584 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Enterocloster sp. isolate MAG1261
            NODE_25978_length_1584_cov_6.372893, whole genome shotgun sequence.
ACCESSION   DBMDUH010000245 DBMDUH010000000
VERSION     DBMDUH010000245.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508360
            Sequence Read Archive: ERR10114003
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Enterocloster sp. (human gut metagenome)
  ORGANISM  Enterocloster sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Enterocloster.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 5.9809x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:54:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,629
            CDSs (total)                      :: 2,584
            Genes (coding)                    :: 2,543
            CDSs (with protein)               :: 2,543
            Genes (RNA)                       :: 45
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 12 of 41
            Pseudo Genes (incomplete)         :: 26 of 41
            Pseudo Genes (internal stop)      :: 8 of 41
            Pseudo Genes (multiple problems)  :: 5 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Enterocloster sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25978_length_1584_cov_6.372893"
                     /isolate="MAG1261"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2719315"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            67..1494
                     /locus_tag="ACZPCG_13145"
     CDS             67..1494
                     /locus_tag="ACZPCG_13145"
                     /inference="COORDINATES: protein motif:HMM:NF024808.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR1286030.1"
                     /translation="MMKKKVIATTILSAAILGSLTGCGGVKTTDDSVVSTGAAEAATE
                     AKEETKATEKAAEGEKVVTFWHSLNGSAGEALETVISNYNEGQGKEKGIHVNLVFQGY
                     EGTDKAILAYQTDDRKNAPDINQGLTSTIPSMMDLSWTVDLTPWIEKEENAVKKDDFY
                     EPMLRSCTADGKLMAVPFANSNMLLYYNEDALKEAGFDAPPATWDELAQYTEALTKKG
                     ADGSVERYGFEAQIKRYHLVNYIVQQSEDSFVGDNEGGRAGEMTKLTIGEDGTLKTVL
                     EKVDALLKTGGYKYVEDSLNEEFANGLTAMCMMSSSRLATIDGLVGDSFQWKAAPIPK
                     ITEADGSGAALGGSCLTLFDLGDEKKVEAAWDVLQYCSSPEAQYILSTGSGYIPVNNQ
                     VEEMDEMKAYYEEHPEFKVPLDQMKSSSPLAQEPLDLVYNEINGVMTDLMLQFCEGSL
                     TIDETVDAIVGECNALLDEYHAAND"
BASE COUNT          461 a          340 c          437 g          346 t
ORIGIN      
        1 ggagctgtaa agggctgatg ctacagacgt aaacgtacat aatgacatgt aagaaaggaa
       61 tacaagatga tgaaaaagaa agtgattgct acaaccattc tctcagcggc aattcttggt
      121 tctctcaccg gctgcggagg cgtcaagaca acagatgaca gtgttgtgtc caccggggca
      181 gccgaggcgg caacagaagc gaaagaggaa acaaaggcca cagaaaaagc ggcagaggga
      241 gaaaaagttg ttaccttctg gcacagctta aacggttctg cgggagaggc gctggagact
      301 gtcatttcta attataatga ggggcaggga aaggaaaagg gcatccatgt aaacctggtg
      361 ttccagggct atgagggaac agataaggcg attttagcgt accagaccga tgaccggaaa
      421 aatgctcccg acatcaacca gggactgacc tctaccatcc ccagcatgat ggatttaagc
      481 tggaccgtcg atcttacccc gtggattgaa aaggaagaaa acgcagtaaa aaaagacgat
      541 ttctatgagc ctatgctccg ttcctgcaca gcagacggaa agctgatggc ggttcccttt
      601 gcaaactcca atatgcttct ttactacaat gaggatgcgt taaaggaggc gggctttgat
      661 gctccgcctg ctacctggga tgagctggca cagtatacgg aggcgcttac aaagaagggc
      721 gcagacggca gtgtagagcg ttacggcttt gaagctcaga ttaagagata ccatctggtg
      781 aactatattg tacagcagtc cgaggactcc tttgtgggtg ataatgaggg cggccgtgca
      841 ggagaaatga caaagcttac cattggtgag gacggcacct taaaaaccgt tctggaaaag
      901 gtagacgccc ttttaaagac aggcggttat aagtatgtgg aagacagtct gaatgaggaa
      961 tttgccaatg gccttaccgc catgtgcatg atgtcctcct cccgtcttgc caccattgac
     1021 ggcctggtag gcgattcctt ccagtggaag gcagctccta ttccaaagat caccgaagca
     1081 gacggaagcg gagcagctct gggcggttcc tgcctgacct tgtttgatct gggtgatgag
     1141 aaaaaggtag aggcagcctg ggatgtgctc cagtactgct cctctcctga ggcacagtat
     1201 atcctttcta ccggttccgg ctatattcct gtcaataacc aggtagaaga aatggatgag
     1261 atgaaagctt attatgagga gcatcctgag tttaaggttc ctctggatca gatgaagagc
     1321 tcttcccctc tggcacagga gcctctggat ctggtatata atgaaatcaa cggcgtaatg
     1381 acagacctga tgctgcagtt ctgcgaggga agtctgacca tagatgaaac tgtagatgca
     1441 atcgtaggag aatgcaacgc tctgttagac gagtatcatg ctgctaacga ctaggaaaaa
     1501 tatgacagaa aaaagaaaaa aagacatacg tcttgttgtg agcgacattg acggaaccct
     1561 gatccgcaga ggagaagggt tccc
//
